Document Qg41QK1v4EDwNb4BDbMn9b7gE

i .. i ` INTERNATIONAL LBAD ZINC RIBKARCH ORGANIZATION *i j! . ''i -vV* OHMOI P. PAOTM M MA0t0 *V|*Vl, dtW f, H. V. 0*0* l*tOH * 8epteeber 1, 1965 TOs All Mesher* of tbe UA-UlflO Load Health and Safety C--lttee nCHi Doo 0. Povltr StSJKTTi 1965 Budget Meeting of lead Health and Safety Co***Ittee; October 1, 1965i Sixty Em Clubi Bev York City The ImA Heelth and fiafety CcMalttee vlll bold lt I966 Budget Heeting at tbe location specified above 00 Prlday, October 1, at previously announced. Project SuaMarlet and Ertea*loo PTopoaal* to b* considered at tbe Meeting re eDcloeed cr vlll be leered m oao u poetlble. Tbe oev Project SuHnarlet replace the Catalog* of fteiearch Issued prior to previous budget Beet Inge. Ve tope you vlll find tbit nev foraet nor* eoovenlent for project rerlev. Pleate bring the encloturet to tbe Meeting. Project or *cal-annual report* are being forwarded to 700 at oon at they are received by XLZBO. Many have already been tent, and ve hope to be able to forward the reaalnder In tine for you to rerlev thee prior to tbe Meeting. An Agenda for tbe Meeting It attached. Ve look forward to teeing you on tbe let. eci Lead Technical Steering Coemlttee EnelI a. Agenda Including lilting of eooloeuret b. IlZfO Project Suanarle* (for all currently active projeete, active project* being ceosldered for extension and nev project* being reecBotaded). r 1966 BCCOET KEZTOa of rax t LIA-ILZflO LEAD HEALTH AID SABOT OOMaTTIX Datei frldayj October 1, 1965 Tinsj 9:30 a.. fUet Sixty ut Club, 60 But V2 Strest, Nv Tort City u. * I. Meeting Celled To Order z r. ,r;< H. Approval cf Mlautes of Previous Meeting * in. l**d Information Program. Report tal Budget for I966. rjr- IV. Q&lted States Public Health Service Symposium on Total Load Coctaalnet100 of the Envlroonent J v. Becent occurrences In the prM VI. Otbar business c. V- :Q vn. 1966 il z p o bu d g et F " 3 f55~~ Proposal or r Project Requested Work SUtenent Title and Contractor 1**75 135*000 Tss Bwu Expoeurv to Particulate Lead Ccopound* la the Air Ooder Experl* mental Conditions; University of Cincinnati feUi LH-79 (c m 10 Toxicity Testing for Organo*Lsed Ccmpowds; Industrial Bto-Tt Leb< oratories, lac. aod The Bine Lab* oratories, lac. Lead Olates for Dlnnej-warej Rutgers, The State University : Study of the Biological Basis for Lead Intoxication; Harvard Medical School \ Texlclty Study of Ingested Paint 1i- N 433.01 PROJtcT HO, La-73 ILZflO PROJECT SIHttKY Iiiut Eatej 8ept*aber 1, 1965 TITHE - Susan Exposure to Particulate Lead Conpound* la tii* Air Ubder Expertnental Conditions PpCTTptB ILB/IITICATIOW - LC-73 Hvan Exposure to AoMent Air Concentrations of k- Particulate Lead OCffTRACTOR - Cblvarelty of Cincinnati, Cincinnati, Ohio curaatT cotmcT - 1/1/65-12/31/65, $35,000, 1965 Budget j CUMULATIVE ECPPt3E3 (to JvM 30, 1965) $06,000 s 1966 PROPOSED OR COKTPJCT PRICE $33,000 1966 PROPOSED BUDGET $33,000 I *966 AUTBORgATTOB irM A Prior Budgets Ubccoaltted loot V- f I966 Projc*J Budget lfad*ilgrated Research rund $35,000 goo* 1966 Proposed or Contract Prlc* $35,665 APPUCATIK13 Direct to ^*olla* additive, end Indirectly to ao*t other usea of lead. CBVJU-T1VE - Defend gasoline additive earlet. j RESEARCH REYTPI <5 H Current Statm - Eat In*ted 00* Half to tvo thirds work acconpllshed. H ] 2. Projected Research - Continue needed to Insure this garket* L 1 3. Reports Issued (a) ihrogreas Reports l_-Rusher of progress reports Issued fron 1/1/63 to 8/19/65, three 2 - Last report go.' 3 - dates of period covered 6/1/64 to 6/1/65 (b) Proposal -(LH-75)PRCPOSAL TO UnTJaATTCKAL LEAD ZIBC 0mA5XE*nC po r t ee cosrruim.-^a, t h j c u o b t h e y ear 1966, cr t h e rnuoacia l s u ppo r t o p t h e PROCPAM OP RESEARCH OP THE KETTERBir) LABORATORY, El THE DEPARXXfJfT OP ET/IPC* KDfTAL HEALTH* OP TCZ COLLEGE OP KEDICDTE OP THE WIVERSITT OP CEUDHATI, <*" THE PKdKCT "HtVAil EXPOSURE TO PARTICULATE LEAD COHTOUOG IS THE AIR WEES KPERIRHfTAL CCUDmOID* ( Off lelally changed, as of July 1, I965, to that above.) (0) Wort Otsteeent * goo* E pgmoFwwr ppv t t w : ! FITotTFroductlcn - got applicable r~. 2. Coreuo*r Evaluatloo - got applicable Jf . Licensing Activities - got applicable . Patents - got applicable 5* Publications - got sppllcshl* el ILAB0 - Son* b) Dsvelopsent Association - goo* -UBf-""Jg! gy.W-V N 433.02 Sj igati vli^If f.'WrV..' * *.; y, .`.... if 2 naro p k u x c t auwmsf - ib t j ECCWOGC PtCPfm II Warint Goal* - Mtlatala world n*rk*t. 2. Appl|ctloc *od H*rt*t Volua* Dm m g**olla* *dditlv* 3- Blf of H*rkt pfDdlxm - 200,000 too* of l**d la O.8.A. *ad 51,000 too* la lurop*. Cccptltlr* Thrtat - Ovang* rafinl&j t*chalqu* to tlialnat* 1*4 addltlv**. >,.( v: ?> # mao f b o j b c t stMwer !a Datei 8*pteebr X, 1965 PBOJKT SO. - IB-T9 Trft> - Toxicity Testing for Orgaoo*Leed Compounds PRmooa ICBfrmcXTiai - LC-79 - Toxicity Testing for Organo-Iead Compound* COffTRACTOB - Industrial Bio-Test Laboratories, Zbs . The Bln* laboratories Id o . CUPJjyr OCBTPACT - Bo contract needed* ctKtmrivT txpjstsss (to j v m j o , 1965) 1,650 19& PROPOSED OR OCWTRACT PRICE - loo* X966 PROPOSES BUPOET 1966 kimppTZKTwa 196$4 Prior budgets Obcoatltted 1966 Proposed Budget On*igDat*d Research food 1966 Proposed or Contract Price 8,350 *5,000 BOO* *5,000 Hmxgf AFPIJC*TICg3 Lubricants, biocides, fungicides, and other* motioned for LC-lQ end LC-2S. OBJECTIVE - To provide toxic classification that vlll satisfy Itgel raqulxsmets for nev mteriele. RESEARCH CTVHV IT Current Btetue 8lx ccapouDde here been completed. * Projected Fbseesrch - To teet ee confound* becoM eveliable through MO eod Ratick. ' 3. Report* Issued W]ProgreesTWport* * Bern (b) Proposal - Boo* (c) Work Otataaeot - Bon* ptmopxgrr progv IT Pilot Productloo - Hot applicable 2, Ccnewer Evaluation Rot applicable 3. Licensing Activities - Rot applicable b. Patent# Rot applicable 5. Publication* Rot applicable (a) XLZ.nO Ron* (b) Dsvslopneot Assoclallon Ron* MtOoale Ch LC-18 and LC-26, 2. Application* and Market Voltes* 0*e LC-lfl and LC-26, 3< flUe of Market Defsndlng 8*e LC-lfl and LC-26. b. Coap*tjtive Threat 8#e LC-lfl and LC-2fl. ? l&ip % 'Hlit4EfjW'' ,.* * w; -1vwJiy-atna N 433.03  .. mi ii Ini. . I , - . . . , nZPO PBOJKCT StMWff liim Detet Beptexber 1, I969 WCJKT WO. - LH-&S TITLE - Lead Olaxea for DtaorrMZ* p r e h o o t HayrmcATtac - ULC-13 - Lead OUmi far DlaMtvare cqrrmcitm - Rutgere, Hm 8tete Cblvereity ctffiHBrr carmcr - 9/1/65-6/31/66# *7,178# 1965 Budget CQHULATIVg EEPgfSlS (to Ju m 30, 1965) 1966 ncPOSED OR COTTPACT PRICK - BOM *3,500 1966 PROPOSED BCDOg - Bo m 1966 AOTBORCATIOa 1965 fc Prior Budget# Obcamltted 1966 Proposed Budget UndealgMted Reeearch fuad 1966 Proposed or Contract Price Boo* Boo* >1,653 Bo m MARKET APPHCATI0W3 - All drillt<J countries use (1a ted dlnnervere. OBJECTIVE - To defend pretest mrket end expend leed uee by assurances of aefrty vith proper usage. RESEARCH manr Ti Current Statue - Balf of the proposed vork 1* coupleted ee of now. 2. Protected Research - Concluding peer of Wo peer project* 3. Reports Issued te> Progress report* 1 - Kinter of prc^mi reporte Issued troa to 6/1/65, Wo 2 - Lest report Bo. 2 - detee of period ccnrered 3/1/65 to 6/1/65 (b) Propoeel - not eppUceble (0) Wort StetcMnt - Bo m pevnopwar t o w n Pilot Product loo - Hot applicable 2. Cooeuner Evaluation Mot applicable J. LicensId * Activities - Not applicable . Patents - Bat applicable 3* Publications (a) tlZflO - Bo m (bj Development AaeoclatlOM Bo m - pxwoycc nctmynt 1. Mariet Quale - AH fisted china dlnnervere throughout the world, 2* Application# and >rVet Vpluae - Bo data 00 voridvid# uss*a. 3# Cite of Market Defending - 16,000 to 20,000 tone M lead In 0.9Jl, b, Coppetltlre Threat . Bon-laad conUlnln* flatee. Threat of *overMeot restriction la eeveral countrlss. i -i- -*-:4> i' - -- - - wi.miyJ^ji 'mill M -bmijiiI'lUJI *ewr' xl ,i , nWB,y.,w,,. i . n W ... , mm',' r 'fTiimp.iwfliw^ai mm w ptytg.' gia. j jap f w y y j f ^ *pg^ IIZBO PROJECT StWAfff Iaaua DtU| September 19^9 PBQJKT BO. - ia-9^ TITU - Stud/ at th# Biological tuli for Lead Intoxicatloo previOtB gJ.rffXi'ICATICW - ULC-16 - Stud/ of tba Biological Baal* for Land Intendcatloo CfltfTftACTOB - Harvard Hedleal School coranrr cotreucr - 1/1/65*12/31/65. 43*,28o.26, 1963 Bud*t CtMltATIVT rxpgces (to Jua* 30, 1965) - ia.y70.26 1966 PSOPOSTO OS COffTRACT PRICE $34,200.26 1966 PBQPQSED BUDGET 43^,260.26 1966 AOT30PJ7>TICa 1965 fc Prior Budget* Ubcoealttad 1966 Pro?o**d Budget tJnd**lgnatad A**aarcb Puod 1966 Propoaad or Cootract Prior boo * $34,260.26 nco* $34,6o.5' HtHOT APPUCATIOiO - To U l**d nartot* and uaat. OBJECTXVI - To provld* fuodaaaotal understanding of th* toxic aod* of actloo of load In biological i/stac*. m'tAxn vamm 1. dui-rcct Statu* * A atart baa been sad* In tba daralopoeot at tool* and tacbnlqua* of investigation. Application la balng cad* to blood, protals and ntya* systaaa. 2. Pro>ct*d ft**arch - Cootlnua as abeva. 3* Report* Iued ta) Prcgre** Baporta 1 -flierar of progr*** report* Issued frc*a 1/1/65 to 8/1/65, oo*. 2 - Laxt report Bo. 1 - data* of period covered 4/1/65 to 8/1/65 (4) Froppaal I4.94 Propoaal for Tba Study of Tba Biological Baa la for Lead let icgreC* StaUnant . Boo* PtmOPKCTT RTVTTM 1. Pilot {Yoductloo Hot appllcabla 2. Cocatsaar Ivaluation - Bot appllcabl* J. Licensing Activities - Bot appllcabl* . Patents - Bot appllcabl* 5. PvZlteattOM - Bot appllcabl* fa) J17JO - Boo* (b) Development Aaaoclatlooa - Boo* prewcafic nrrmyi 1. >ar..t Coala Da fan** of nerAats aucb a* Ttt, paint, can aoldar, prasanl1/ a* sal lad ty allegation* of toxlo reaction. Expand lead u*a Into area*, auca a* auto body painting, vMra allagatlooa of toxic batarda altlgate against use. iy n MW w.iw**i y af ij 11^14711/1. if'"1 ijift.-n'FMPJM.jjHJ %*qwgf4rv' - N 433.04 *"V ()ir^a8---'-^^MpVlSOTItVVriTfgi! urteximi ,*att-,v m.**,VjmiKtMam -Yrt^jhr-' -a- g**0 PROJECT KHWff - 2. Application* ad Htrtrt Voluna . All lead uaaa. 3 61f of Marfcat Dafeadlnjt - Applicative to all load rrtttt. k' Itmt . J>f*alv* agalaat corpatltlco and lagal raatrlctloo*. '::i , ^'w-w^M|-.ig(ll.!|mug. IL ;_-v. i is PPOJBCT 1W. - LH-IC* TITU - Toxicity Study of Ingested Point Pita mvnxJ3 IDOmnCATXCW - UtH-lJ - Toxicity Study of Ingested Point FUa CCTTRACTOH Bsileton laboratories, Incorporated omyarr c o t -p a c t - Scm CUMULATIVE PCPBngl (to Jvne 30, 1965) - loot 1965 PROPOSES OB COTTBACT PRICE $9,900 1966 PROPOSE) BUDGET - See* 1966 ATTBOPEATiai i:^5 1 Prior Budget* UQcemitted 1966 Pro^Ml Budget Ubdeslgneted Research fund 1966 Proposed cr Contract Price $9,900 Boo* Kern* 13^53 HtSXET APPUCATICB3 - Point pimento. oajjrrm - To raise fran li total load the present restriction 00 point for Interior u m. To help create o better c Haste of opinion for lead point* in general. RESEARCH mvro 1. Current Statue - Contract not signed. 2. Projected Research - Joint sponsorship vith Rational Point, Tarnish and lacquer Association and acrlce fron several governs*nt agencies. 3. Reports Issued () Progress Reports - Soc* (b) Proposal - Sons to be considered.' ' - (c) Vort statement - Bot applicable PEvnflrxm nvrm 1. FTToTTrouJctlon * Sot applicable 2. Consumer Kvaliatloo - Sot applicable 3. Licensing Activities - Sot applicable b. Patents - Sot applicable J. Publication# Sot applicable (*) IUj O - Sooe (b) tvelopc*nt Assoc 1st lor* . Sons * troncMic nrrwrnvr 1. Hrt ft<*l Prlnaryi Interior paints, vith estimated potential of 10,000 tone nee le4*uee. Ceccodaryi Miscellaneous uses, such ae auto body finishes. ? 2. Apf-1 teat tens and H*r*et Volur* * Paint use Is Increasing vith present sales In excess of tvo billion dollars'per yoar, . oLi- hk* \c- &m**i*aFz**-' 'ipm- v SfPSW IN' . .iJL!li',HJ,j,n unwm'.wtJ N 433.05 ' @ ; nzpo PBDJBCT 8U*UKT - 3. S'.t* of >frrfa>t Defending - Preaent 0.8. about 50,000 too* par year. PROGRESS REPORT - PROJECT NO. LH-75 INVESTIGATION OF HUMAN EXPOSURE TO PARTICULATE LEAD COMPOUNDS IN THE AIR UNDER EXPERIMENTAL CONDITIONS The work outlined In previous reports of progress, the lest of which wee dated September 10, 1964, has bern directed primarily toward the determination of the maximum concentration of ideally respirable (that is, particles of lead too small to be deposited In the upper respiratory tract) inorganic lead in the atmos phere, that can be inhaled continuously throughout a lifetime by normal, healthy persons, without demonstrably progressive accumulation of lead in their bodily tissues. The approach to this problem is patterned on the results of balance experiments (intake of lead in food and beverages, and output in the feces and urine) carried out previously with human subjects under various conditions. In the present experiment, such subjects, after preliminary observation of their lead metabolism under normal conditions, are subjected to the inhalation of air con* taining lead in a known concentration over periods of time sufficient to demon strate the occurrence of non-occurrence of a physiological response in the form of an increase in the urinary output of lead, (This response can be detected before any other in the intact human organism. ) It is postulated, on good grounds, that the response of human beings to continuous exposure of this type can be determined with approximate accuracy by extrapolation from the results of rsgular incremental increases in the duration of intermittent exposure - that is, x hours of exposure per day for a suitable time, followed by } jx^and 4x hours of expoaure, for com parable periods of time, will yield a series of points on a cutve which can be tfmmm&mrm N 433.06 extended to 24 hour* per day, every day. . In a continuation of previoua experiment*, Subject* N. K. and S. 5., in A essentially parallel experiment*, have been subjected to exposure to lead sesqui- oxide dl*per**d In the air of the two reiplratory chamber* In particle* ranging from 0.01 to 0.18 micron In diameter (mean *lte 0,05 micron) in the concentra tion of approximately 10 mlcrogramf of lead per cubic meter. (After preliminary control observation*, over a period of month* prior to the year 1965), the** j subject* were Introduced into the chamber* for 3 hour* on every other day (10.5 fj- --i hour* per week) over the period of 12 week*. Subsequently the duration of the r,. I exposure per day wa# doubled, trebled, and further multiplied until, at present, August 18, 1965, one of the subject* (H. K. ) 1* spending 63,0 hour* per week, in the chamber, white the other (S.S., who started a bit later) i* spending 52.5 hours per week, both having passed through periods of approximately 16 weeks of exposure for 10.5, 21, 31.5 and 42 hour* per week, respectively, while Subject N. K. wa* subject to, additionally 52.5 hour* per week for 16 weeks, before under going the present phase of the experiment. There ha* been no demonstrable excretory response on the part of either subject to the experimental regimen. That 1* to *ay that the quantity of lead absorbed dsity In the lungs of these subjects has not been sufficient under these condition* lobe demonstrated in the face of the variability of the urinary excretion of lead from day to day Induced by the absorption from the alimentary tract. Or to be etlll more specific, iht quantities of lead absorbed from the alimentary tract from day to day are so much larger (they can be expectsd to be much more variable, ! aleo) than the quantities absorbed dally from the lung that ths latter cannot be f. detected. i i; --F. - It 1* necessary to obtain * response, and In fact to obtain a series of increasing excretory responses, In order to obtain the data for extrapolation from intermittent to continuous exposure. Therefore the experiment must be continued in increasing increments of severity (concentration of lead in air and duration of exposure) until such data have been obtained. One would wish to expedite the experimental procedure so as to obtain definitive results more promptly and at lesser cost. However, in view of the total lack of experimental precedent, and because of the risk of overshooting the rmrk (in terms of concentration of lead in air) and thus rendering the subjects unfit for further observations, it seems wise, at this time, to follow the original experimental design during the coming year. If this is done, and U it is necessary, as seems likely, to go beyond the level of 10 micrograms of lead per cubic meter of air in order to obtain an excretory response, the procedure will be that of beginning the new levet of exposure at a point, in terms of concentration and time, which is judged to be equivalent to the next serial increment of Increase in Inten sity. Ones a response has then been obtained and stabilised (other data indicate that a plateau of response will have been reached in 16 weeks, at most), the course Is set for the addition of further points in a curve which will yield the approximate value for continuous exposure. There does not appear, at this time, to be a short cut to this result, and in view of the Investment of time and money in this experi ment and in the metabolic evidence on which the experiment is foundedfit would seem unwise, now, to alter the design. However, at the end of this year (196)), the experimental data will be reviewed critically, as a means of checking the ex'perimentel design and of affecting such reduction of time, effort and monetary expenditure as may be feasible. Such modification of procedure as may seem feasible and wise will be communicated to the sponsors for comment and criticism. o * to achieve the maximum benefit from the collective critique. Considering the preeent situation of the principal Investigator (on leave In the service of Pan. American Health Organisation for the next three months) as well as the present stage of the experiment) it is believed that no useful purpose would be served at this time by assembling the data that would merely illustrate the negative results of the experiment as conducted up to this point. It should be emphasised, perhaps, that the very negativity of the results, in relation to the point of significance that remains to be established is an extremely Important finding, Robert AA. Kehoe, hi. O, Principal Investigator Date: August 18, 1965 ij I PROPOSAL TO INTERNATIONAL LEAD ZINC ORGANIZATION FOR THE CONTINUATION. THROUGH THE YEAR 1966. OF THE FINANCIAL SUPPORT OF THE PROGRAM OF RESEARCH OF THE KETTERING LABORATORY. IN THE DEPARTMENT OF ENVIRONMENTAL HEALTH* OF THE COLLEGE OF MEDICINE OF THE UNIVERSITY OF CINCINNATI, ON THE PROJECT "HUMAN EXPOSURE TO PARTICULATE LEAD COMPOUNDS IN THE AIR UNDER EXPERtMENTAL CONDITIONS" On October ), 1962, ths general experimental procedure of this project, together with the details of a epeclflc experiment to be carried out in 196). were described in detail. Further proposals, relating to the continuation of the work, were made on September 4, 196) and on September 10, 1964. The work has gone on in essential accordance with the purposes and procedure described in these proposals, and is to be continued in the year 1966 along similar lines indicated herein. The Immediate goal of the sxperimertal work in 1965 was that of defining, through human experience, the maximum concentration of ideally respirable and absorbable (i.e., pulmonary absorption, exclusively) particulate lead in the ambient atmosphere to which normal, healthy, human subjects might be subjected, con* tlnuously and for indefinitely prolonged periods of time, without incurring the potential risk of a demonstrable degree of accumulation of lead In their bodily - tissues. The experimental principle Involved the exposure of two human subjects, In parallel respiratory chambers, to air <ont.>lnlng lead (approximately 10 micro * Ofliclalty changed, as of July I, |9?5, to that above. K .i* N 433.07 gram* of Pb( a* toad seaquloxlde, par cubic meter) dispersed in minute particle* (ranging in diameter from !e* than 0,01 to 0, 18 micron*, with the mean diameter of 0.05 micron*), on an intermittent sebedul* of lncreating duration (3 hour* per dap every other day for approximately 16 week*, I. e., 10.5 hour* per week; 6 hour* per day every other day for approximately 16 week*, i. e., 21.0 hour* per week; and *o on to 31.5, 42.0 hour*, etc. ) until the effect of the expoture became evident through a (light (ststlstically demonitrable) increaie in the output or con* centratlon of tead in the urine, preiumabty (a* judged by earlier experiment*) without demonstrable increase in the concentration of lead in the blood, of the aubject*. During 1965, the** observation* have continued a* scheduled, except for necessary adaptation of the period* of exposure to the tolerance of the subject* for disruption* of their daily live*. That is, when the schedule reached the point of requiring the subject* to spend more than 12 hour* per day in the chamber (on alternate day*) it became necessary to shift to daily period* of exposure. Obvious* ly, al*o, the continuation of the famity life of the subject* required,at least, on* day per week of freedom from exposure. The result wa* a new schedule, involving the proper number of hour* of exposure per week subdivided into equivalent period* on six of the seven day* o/ each week. At this time (August 1965), on* of the subject* (N. K. ) I* spending 63 hour* per week In the chamber, white the other (?.S.) (who was taken on tat* a* an experimental subject, as reported prevloualy) is ipendlng 52.50 hours per week. Inasmuch a* neither of the** subjects has yielded the slightest response to th# graduated levels of experimental expoaure, the experiment la to continue a* long aa possible at th* present level of lesd concentration in air. It I* not anticipated *t E jyyfuijwgfwi i preaent that it can go Ur beyond 12 hour* per day on 6 day* per week, with due regard for the comfort and convenience of the aubjecta. If then no reaponae ahail have been obtained, the concentration of lead in the chamber will be increaeed by a email Increment, and the Incremental increaaea in the duration of expoaure will be reacheduled, beginning at a level of Intenaity (time x concentration) that will not duplicate that of previoua atepa. It will be necesaary, obvioualy, to obtain a reaponae, and auch reaponae muat be graduated from the leaat demonatrable intenaity through at leaat three tevela in exceea of the minimum. In order to provide data for extrapolation to the effecta of.continuoua expoaure (24 bourr per day on 7 daya per week). Accordingly, the experiment may be expected to continue through the entire year 1966. It la conceivable that the experimental regimen can be modified ao aa to ahorten the time required to obtain definitive reaulta. The meana of ao doing are under conaideration at prerent, and the entire eituation will be explored critically at the end of thia calendar year. There ia aome riak, however, in any radical alteration of the original deaign of the experiment, in that too rharpan increaae in the expoaure may yield an exceaaive reaponae, and threaten, thereby, the further uaefulneaa of the aubjecta. It waa for thia reaaon that the initial level of the con centration of lead in the chamber waa aet at 10 mlcrograma per cubic meter - a level which could be counted on (from Indirect but aatiafactory evidence) |o yield negative reaulta at fir at, with the poe.ibllity that there might be a poattlve reaponae aa the time factor progreaaed toward continuity. .. With the present facta and anticipation a In mind, the coat of the expert mental program in 1966 la expected to be almilar to that of 1964, Some economy rmy be affected, elnce it aeema unneceaaary, in future experlmenta, to analyae - . - -w-- duplicate samples of the food of the subjects, (The correlation between the lead in the food, day by day, with that in the fecee, day by day, ia o eatlafactory ae to enable the former item* of data to be eliminated, apparently without introducing an error that ie greater than the aampllng error. Further atatietical work wilt be neceeeary to aubetantiate the facta in thia regard with certainty, and thia feature of the dealgn will be examined critically at the end of the current year (1965). Only one other iaaue ariaea at thia time in the budgetary conaiderationa. The propoaal to the Public Health Service, involving their further participation in the aupport of thia project, hae been approved in principle, but ia dependent upon the availability of funda through Congreaaional appropriation, it ia expected that the funda will be made available, but ahould thia not be the caae, it will be necee* aary either to eubmlt a eupplementary propoaal to the other eponaora of the program, or to curtail the work. The following budget ia recommended for 1966, on the baaia of the expen* dlturea made ia 1964, and of thoae made to dale in 1965: For peraonnet and aervicea (direct and indirect coata) For apecial reagents, aupptlea, allowances for food, etc. Overhead (proportional share of cost of operating the Laboratory) $ 75,000.00 5,000.00 JO. 000. 00 Total $110,000.00 On the assumption that funda wilt be available from previous sources for the aupport of this work, the costa would be allocated in round numbers, as follow#: Ethyl Corporation E. 1. du Pont de Nemours and Company International Lead 7.Inc Research Organisation U. S. Public Health Service 115, 000, 00 lb, 000.00 >5,000.00 l4,000,00 Total $109,000.00 White the roughly estimated budget and the equally roughly estimated iH 'w&ST? a?*J ' -.'v'1 esawaaeaigM^iwisT.. amMSMe mb s c j w w ? , <* tgwsi*!-iaaw.t.w.-.1-wwawM !u.i>MWiM t L. ' can be held within tha estimate. ; Data: August 18, 1965 RoberbA. Kehoe Kettering Laboratory Principal Investigator a &J H Sa-^ ***** i 1.