Document QbNe03qKDn800ggdzbBNRGQR

A R 023-00 7^ LaboratoiyReport Analysis of Fluorochemicals in Wild Bird Livers Laboratoiy Report No. FACT-GEN-010 LRU2392 Testing Laboratory 3M Enviro3nMmEenntvailroTnemchennotalolgLyab&orSaatoferyty Services Fluorine Analytical Chemistry Team (FACT) S9t3. P5aBu2ul-,3sMhE-AN0v95e5n1u0e,6 Laboratory Contact SPthKPoanrPBuies.l:lO,HdM(g.a6B.nN52os1-ex53)n75E3,713-P3803h39-1.6-D30.31381 FAX: (651) 778-6176 Requester 000520 3MENVIRROENPOMRETNNTOAL. FLAACBTO-RGAETNO0R10Y Sixty liver samples collected from various species of birds were characterized with respect to specific fluorochemicals. The birds were classified according to specie and geographic location. Six species of birds are represented in this study, including Sandhill Cranes, Double-Crested Cormorants, White Pelicans, Brown Pelicans, Great Blue Herons, and Brandt's Cormorants. The bird were found at a variety of sites across the United States including Nebraska, New Mexico, Florida, Arizona, California, Louisiana, and Florida. In general, the cause of death to these birds is unknown. Details about the location each bird was found and about die cause of death (if known) are included in Appendix C of this report. Liver samples were homogenized and extracted using an ion-pairing extraction procedure. The extracts were quantitatively analyzed for PFOS and PFOSA using high-pressure liquid chromatography/electrospray tandem mass spectrometry (HLPC/ESMSMS), and evaluated versus a standard curve extracted from rabbit liver. Analytical details are available in the study binder maintained by FACT in the 3M Environmental Lab. The presence or absence of other known fluorochemical contaminants and metabolites (PFOSAA, M556, M570, POAA, M513, PFHS, and PFDS) was determined by inspection of HPLC/ESMSMS analysis of 2 bird livers within each group. A frill list of acronyms is attached in Appendix B. fWroimth tthhee leixvceerspotiofanlol ofmfthoestboirfdthsewSearenddheitlelrCmrianneedstaoncdoantsainingPleFBOrSowabnoPveelitchaenmfreotmhoCd alilmifoitronifaq,uthaenteixtattriaocnts (M DL=10ppb). Extracts from the livers ofthree of 15 Sandhill Cranes showed some PFOS; two ofthese extracts contained PFOS levels very near the MDL. EcsrehlxamotsrwsoaivecfidetcsdaPftaFrioonOmndSP(o>lFne2Ove0eSBxlsrflaeoinvnredtghltsr'eeswacCtoitoerhmxrimcnoeoarsarspanoantrftatftinhrcodoumslnaeerSadcraellantyesDrslmOiifeixicgnafoeot,diroCitnnhAaethrapeeneadelvixcoetanrrnaae)cgW.tesdIfhf,ritosthoememPseoeelttihgwceeaornnbeo,irufrardtlolsyomwibnsCigetharpvlioinapittnaihottrseniasas,rabeCmasAeed on the geographical location ofthe birds' habitatcan be made. With the exception fifths Sandhill Panes, in general, liver from birds collected from the western US (San Diego, CA, Calipatria, CA,) show relatively low levels ofPFOS, with an average ofless than 50 ppb. Livers fPwrFoeOmreSbdlieertdvesremlcso,inlalepedcptrteoodxcoiomnnattahteienlyEth1ae0st5hCipgophabes.sttoElefxvtthreaelscUtosSffPr(oMFmOiabSmi,radi,pNlpivraoeprxlseimsc,oaaltlneedlcytFe3dt0Lf0raopumpdbed.rideaClee,nFtLra)lsUhSow(LmAoadnedraNteV) It is unlikely thatthe differences associated with PFOS levels in the livers are purely a function of species. In mdcoifonfreteareitnhnaatnPnFoaOnveeSrianlgesvetaePnlsFc.eO,FSdoiceroesnxacamemneptsrlpaete,icloiivneesorofs5fcb8oilprldepcblti,evwdinhfgrioliemnlaiwvdehirfisfteecropelenlelticcetanenvdsifrirononmCmaWelinfthosirthneoiPawewelidecraseingdsneiitnfeirNcmaenivntlaeyddato showed average PFOS levels of over 350 ppb. Sandhill Cranes are an exceptionto this observation, as the livers from these birds were determined to be uniformly taw in PFOS, regardless ofthe geographic location ofthe birds. W ith the exception ofthe Sandhill Cranes, liver extracts from at least one ofthe two birds screened within edSaeactnehdrmhclialilnsPesdirfuitconaectioonnatreawripne. draeetsdemertmaelrilnmaemidnoetrdu>nctootnoctfoaPninFtaOainSsmPAFa.DllNSao.mPLoOuivnAetrAoef,xPMtrFa5Hc1tSs3.,frMNom5ea5sr6le,yvM2er05a%7l 0sop, oefctrhiPeesF,lOiivnSecArlueAdxitwnragacst1s were detected in the liver extracts from any ofthe birds. Appendix A contains these results. This data indicates thatpotential geographictrends associated withthe environmental distribution ofPFOS and other fluorochemicals maybe traceable by characterization oflow-level ofthese compounds in animal tissue samples. 000521 M ENVIRROEPNOMRETNNTOAL. FLAACBTO-GREANTOOJROY APrlothcoeduugrhersigwoerroeufsoqlluoawlietyd,cdoinetraoclqmuiesaitsiuorneos fatnhdis3dMatEa nwvaisronnomtneenctaelsLsaarbiloyractoolrleycStetdanadcacrodrdOinpgertaotianpgplicable phGrigooohddeuscLttqasbuuoaitrlaiatbtyoildriytaytPaoraravcstaaificleeatsyb..leTRahteitsshudilstasttiasmhiseo.nulodtbseuictaobnlseidfoerreddisttoribbeutsieomn tio-qaunayntgitoavtievren.mDeanttaapgreensceyntienddheeferensisetohfe LOanb5; /s5a/m98p,leJesawneBreurneicsehivoerdnf3roMzeOnc. cTuhpeatsioamnapllMesewdiecrieneordigeliinvaelrlyedsethnet tboirGdeliavreyrOsalsmenpl(e3sMtoOthcecEupnavtiiroonnaml ental Medicine) by ScottP. Hansen ofthe National Wildlife Health Centerin Madison, Wisconsin. There is no holding time criteria associated with these samplesfor LC/ESMSMS analysis. AnFSaAulryCftaiTcc-taaMlnm-ts1ef.0rtho,oEmdxsLtriavcetriofnoroAfPnoatlaysssisiuUmsiPnegrfHluPoLoCct/aEnSeMsuSlfonate or OtherAnionic Fluorochemical FACT-M-2.0, Analysis ofFluorochemicals in LiverExtractUsing HPLC-Electrospray/Mass Spectrometry SaAmnpalleypteriespeaxrtaraticotend-fraoqmueaosuasmspamle pmleastr,iHx wPLitCh /iEonSMpaSirMingSr:eiaogne-npta[itreitnragbeuxtytrlaacmtimononium hydrogen sulfate O(TnBcAe t)h] einanaipoHn--TcoBnAtroplaleirdisenfovrimroendm, tehnetaTnahleytceaitisotnraicnsrefeargreendtinsetoleactnivoenly-ptoalragreotsrgaannioicnsicoflvlueonrto(cehtheymlicals. acetate), dried, and reconstituted in methanolfor MS analysis. H PIcnLo lCHu/mPELnSCM,BaSansaeandlidqounHotPthoLefCaef/xfEitnrSaitMcyt oSisfMitnhSjee:caftnoeadrlydatneetdfaopilarestdhseeqdusttaahltriiotoauntgiavrhyeapwrheoavrsekersien pthheasceolliuqmuindrcehlartoivmeattoogthraeplhiqicu id mobile phase, the analyte is retained for a characteristic amount oftime. For example, in a standard saonlaulytitoenePxFtrOacStemdafryoemlultieveart i8n.0thmisinauntaelsy.siRs weteenretiomnatticmheesdboentwtheeeHnPaLstCansdyasrtedmPFtoOwSistholiunti1o%n.and the ForogllaonwicincgomHpPoLuCndsse,pianrcaltuiodnin,gESflMuoSroMchSepmroicvaildse. sEalercatproidspanradya,cacnuiroanteizmateioannstefcohrnainqauleyzuisnegdapwrimidaerrialynfgoerof detection ofmolecular ions, is generally operated at relatively mild temperatures. Molecules are ionized, and a primary ion, characteristic ofthe analyte, is selected This ion is bombarded wife high-energy gas; sduebtescetqeud.entcollisions create smaller secondary ionic fragments uniqueto theprimary ion, which are . For example, for PFOS (CeFnSOf) analysis, ion 499 is selected as the characteristic primary ion. This ion ithsefisaegsmeceonnteddariyntforaogtmheernitosniss sduectehcatesd8a0nadmcuan(cboerruessepdontodidnigffteoreSnOtisaOte, PanFdO2S3f0roammuo(thCe3rFc6oSm03p')o-uEndascthhoaft mseicgohntdhaarvyeiothnefrsaagmmeecnhtaartiaocntepraistttiecrn4s9.9 amu primary ion, butdifferentchemical compositions and 000522 3MENVIRROENPOMRETNNTOAL. FLAACBTO-RGAETNO0R10Y *** *w * -w t *A * % A * * v -*i 4 + <*. * f J s V t", ' v ' v * t X' , v . ' ,./; / m .r s - > U <. ' /m tftft i/j , t A ^ N %s ' is ' >v *< / v .. * %< ' S *** * > * .* > ^' * * ''** * *Y? * * * '"r * Y *&* i * , * > , V > A ^ w d* X - .^ y ^ * .& ^5 JS i< X * > /Xm\ .* r i 4 * w * w m A C a * aCSx i *>5,iy. >A W w ^ y A . +s" **+ 'r * > , , ? s > ' 5.1 Calibration iAnsmtruidm-leenvteall, derxitfrta. cCteadlimbraattriioxnccahliebcrkatsiotanncdhaerdcskasrteancdoamrdpilisaanntaiflyinzestdruemveernyta5l-r1e0spsoamnspeleisswtoimthoinn+it/o-r30% of the expected value. Quantitation ofthe target analytes is based on linear regression analysis, weighted 1/x, of two extracted wmsdpoaaeestcsrniinfxoioccttuduasradveuvedgsehfrbotserrearlPcyioFkanOeftfuSienscgditneetghtaeetcrhmhdeagimntraaoutquiluotpianpol;liefbtysrr.aeamscpkpoelnteissne.g-mQcauolainbnirttoaittraiiontingoncmhooefdcekeasocwhf taehnreeaialnyllstetcriousmmbpaelsnieatdnTti,nhseothsteherciesosenpxdocncluusesrviooenf a 5.2 Blanks TAwddoiteixontraalclyti,otnwbolasnakmsp,uletisliozfinbglawnkatmeraatrsixsu, urrsoegdafteormthaetriexx,tararecteexdtrcaaclitbedrawtioitnhceuarcvhesb,aatcreheoxftsraamctepdleasl.ong with each batch of samples and curves. Blanks are compliantif no target analyte is detected above the limitofdetection for a specific analyte. In this study, allblanks were compliant 5.3 Surrogates Tmeotrnai-tohryeddrotopceornfifuiromrogorcotsasnienssturulfmoneanttealiMs uusreed, iafsneacessusarrroyg. ate in this analysis. Surrogate response is 5.4 Matrix Spikes Dcruapnelicliavteerm. RaetrcioxvsepriikeesaonfaPlFysOeSs uatnildizPinFgOaSllAtawrgeerteawnaitlhyitnesthweearcecperpetpaabrleedraanngdeaonfa7ly0z-e1d30fr%om. Sandhill 5.5 Laboratory Control Samples Laboratory Control Samples are not a component ofthis study. 5.6 Sample Related Comments Matrix spikes were prepared for a single specie ofbird ft is assumedthat spike recoverieswouldbe sreimasiolanrafboler arelltesnpeticoinest.imNeoasntdanredaasrodnmabatleerdiaalufgohrtPerFiDonS sipseacvtaruilmab.leU. nCtiolmstapnoduanrddimdeantteirfiiaclaitsioanviasilbaabslee,d on identification ofthis analyte should be considered to be preliminary. QwausanotbitsaetrivoendfowrhPeFnOmSowniatsorcionngdthuect4e9d9by->m8o0ntirtaonrisnitgiothneintrathnesibtiiordnl4iv9e9r-m> a9t9ri.xS. oTmraenpsoitsiiotinvpeairnatmerfeeterersnfcoer the othertarget analytes are listed below. 000523 3M ENVIRROEPNOMRETNNTOAL. FLAACBTO-RGAETNO0R10Y Table 1: Analyte Transitions for HPLC-ESMSMS Analysis Compound Prim ary Ion T ran sitio n M onitored PFOS 499 99 PFOSA 498 78 PFOSAA 584 169 M556 556 169 M570 570 169. POAA 413 169 M513 469 169 PFHS 399 99 PFDS 599 99 With the exception ofmost ofthe Sandhill Cranes and a single Brown Pelican from California, the extracts f(erMxotmrDaLcthts=ec1loi0vnpetaprsibno)e.fdaEPlxlFtoOrafStchtlseefvbreoirlmdssvtwehreeyrlinevedeaerrstetohrmfetMhinreeDdeLtoo.fc1o5nStaainndPhFilOl CSraabnoevseshthoewmedetshoomdeliPmFiOt oSf;qtwuaonotiftathtieosne Extracts from one Brandt's Cormorantfrom San Diego, CA and one White Pelican, from Calipatria, CA schlaoswsiefidcaPtFioOnS(>le2v0exlsfoinrtghreeactoerxmcoersasnotfatnhdosneeadreltyerlmOxinfeodritnhethpeeelixctarna)c.tsIffrtohmeseotthweorboiurdtlsywinigthpionitnhtseasraeme . trheme goevoegdraanphdiPcaFlOloScaletivoenlsowftihtheinbiardpsa'rhtiacbuiltaartcclaanssbifeicmataiodneare averaged, some general observations based on With die exception ofthe Sandhill Cranes, in general, liverfrom birds collected from the western US (San DPfrFoieOmgSob,lierCdvAsel,csCo, laalpelicpptraeotdrxioiam,nCathtAeel,y)Es1ah0sot5wCpopreabls.attoEivfxetthlryaeclUotswSfr(loeMmvieablmsiroid,fNlPivFaepOrlseSsc,,owallnietdchtFeadnt LfarvaoeumrdaetghredeoaClfeel,enFstLsrat)lhsUahnSoSw(OLmAppoabdn.edrLaNtievVer)s were determined to containthe highestlevels ofPFOS, approximately 300 ppb. It is unlikely thatthe differences associated with PFOS levels in the livers are purely a function of species. In more than one instance, the same species ofbird living in a differentenvironment showed significantly dclsihoivfonefwetrasreiefndnroataPmnvFeatrOvhaeegSrseaelgePbveFierPOldsFSs.OwFleSeovrcreeoelsdnxecoatemfenorptmvrlaeeit,nriole3invd5e0otrofspt5pce8bou.lplnepScibfato,enwrddmhhfrliioylllemlColirwwvaenhirenistsPecaFoprlOeelelaiSccnta,enredexsgfciraneorpmdCtliaeoWlsnisfhtoooirtfntethihPaiesewlogiecberaoseengdrsrveaiatpnethirNomicnei,lvnoaaecsddatathtiooen ofthe birds. eWacithhcthlaesseixficceaptitoionnwoefrtehedeStearnmdhinileldCtroanceosn,tlaivinePr FexDtrSa.ctLsifvroermexattrlaecatsstforonme osfetvheeratwl sopbeciridess,sicnrceleundeindgw1ithin dSeatnedrmhiilnl Cedratonec,ownetareindaetsemrmalilnaemdotouncot onftaPinFOa SsmAalNl aomPoOunAtAof,PM1F5H1S3., MN5ea5r6l,yM205%70a, oftrhPeFlOivSeAr eAxtwraacsts were detected in the liver extracts from any ofthe birds. 000S24 3MENVIRROENPOMRETNNTOAL. FLAACBTO-RGAETNO0R10Y This data indicates that potential geographic trends associated with the environmental distribution ofPFOS atinssduoethsaemr fpluleosr.ochemicals may be traceable by characterization oflow-level of these compounds in animal This reportreflects the mostaccurate data available at this time. All numbers shouldbe considered to be semiquantitative. Samples and extracts willbe retained for future evaluation, if necessary. 9.1 A ttachm ent A: Tables of Qualitative and Quantitative Results from the Analysis o f Liver Samples 9.2 A ttachm ent B: Definitions o f Fluorochemical Acronyms 9.3 9.4 AImAbtattiatysacbcdhehamtmtareaienncnetdaDticbC:aleEt:ebxSsyttarhamcachtptaiploreoantcdeatenensrtdiiczarAaliptgnietoaionolygnctrifaaclpnaohldwdicsa-altteramevnepdllefsluaresoscrooeccipihatetedmdaitwcaailtshitnhaeneinmvairlotinsmsueenstaalmdpisletrsi.bution ofPFOS Harold Johnson, Analytical Chemist Kris*HHaannsseen, PhXDL., Principal Analytical Investigator Date D ate 000525 10/07 '99 08:43 ID:3M ET&S 42-2E FAX:612-778-4468 Analysis of Wild Bird Livers. U* not detected NA not analyzed x " detected LOQ" Limit of Quantitation PAGE Sam ple Species No. 1 Sandhill Crane 2 Sandhill Crane 3 Sandhill Crane 4 Sandhill Crani 5 Sandhi 1Crina 6 ' Sandhi 1Crani 7 .Sandhi 1Crani S Sandhi I Crane 9 Sandhi 1Crane 10 Sandhi i Crane 11 W hite Pelican 12 W hite Pelican 13 W h ite Pelican 14 W hite Pelican 13 W hite Pelican 16 Brandt's Corm orant 17 Brandt's Corm orant 18 Brandt's Corm orant 19 B randt's C orm orant 20 Brandt's Corm orant 21 Dbl. Crested Corm orant 22 Dbl. Created Cormorant 23 Dbl. Crested Cormorant 24 Dbl. Crested Corm orant 25 Dbl. Crested Cormorant 26 B ro w Pelican 27 Brown Pelican 28 Brown Pelican 29 Brown Pelican 30 Brown Pelican 31 Sandhill Crane 32 Sandhill Crane 33 Sandhill Crane 34 Sandhill Crane 35 Sandhill Crane 36 Dbl. Crested Cormorant 37 Dbl. Crested Corm orant 38 DbL Crested Cormorant 39 Dbl. Crested Cormorant 40 Dbl. Crested Cormorant 41 Brown Peliean 42 Brown Pelican 43 Brown Pelican 44 Brown Pelican 45 Brown Pelican 46 Great Blue Heron 47 Great Blue Heron 48 Great Blue Heron 49 Great Blue H en 50 Great Blue Heron 51 W hite Pelican 52 W hite Pelican 33 W hite Pelican 54 W hite Pelican SS W hite Pelican 56 Brown Pelican 57 Brown Pelican 58 Brown Pelican 59 Brown Pelican 60 ' Brown Pelican Location Keamcv. NE Kearney. ME Keamev. NE K earney. N E .... K eam ev. N E Chochise Co.. AZ Chochlee C o., AZ ^h ochlte Co.. AZ C hochise.Co.. A Z Calioatrie. CA Calipatria. CA Calipatria. CA Calipatria. CA Calioatria. CA San Dlexo. CA San Diexo. CA San Dlexo. Ca San Dlexo. CA San Dlexo. CA St. M artinville. LA St. M artinville. LA St. M artinville. LA St. M artinville. LA St. M artinville. LA M iam i. FL M iam i. FL M iami. FL M iam i. FL M iami. FL V alenica Co.. N M V alenlca Co.. N M Socorro Co.. N M Socorro Co.. N M V alenlca Co.. N M Naples. FL Naples. FL Naples. FL Naples, FL Naples. FL Calipatria. CA Calipatria. CA Calipatria. CA Calipatria. CA Calipatria. CA. St. M artinville. LA St. M aninvllle. LA St. M artinville, LA St. M artinville. LA St. M artinville. LA Fallon. NV Fallon. NV Fallon: NV Fallon. N V Fallon. NV Ft. Lauderdale. FL Ft. Lauderdale. FL Ft. Lauderdale. FL Ft. Lauderdale. FL Ft. Lauderdale. FL PFOS PFOSA PFHS rob 41 -<LOQ-- It <LQQ-- _<LOO NA <LQQ _<LOO <IQQ__ < 1 0 0 , NA <LQQ _<LOO NA <LOO _<LOQ 8 <LQQ_ <LOO I <LOO <LOO NA <LOO < 1 00 NA <LOQ-- <LOO NA 35 1293 _<LOO <LOO_ X X 29 _ <LOO NA 15 <LOO NA 153 <LOO NA S3 80 8 46 31 NA 46 34 NA 80 13 NA 2055" 94 X 59 20 145 7 X' 8 333 23 NA 76 <LOO NA 170 _<l o q N A 106 58 8 134 11 8 125 10 NA 159 47 NA 48 11 N A < < lLoO qO L <LOO <LOO 8 8 <LOO <LOO NA <LOQ <LOQ NA <LOO <LOO NA 212 9 8 10 < O S L . 8 PFDS 8 NA 8 NA NA NA NA NA NA NA X XN A NA NA X NA NA NA X X X NA NA NA X X NA NA NA 8 8 NA NA NA X 8 32 100 152 16 36 <LOQ 6 32 188 59 1061 261 173 141 362 927 133 291 194 75 71 31 91 . <LOO NA NA 35 NA NA 24 NA NA <LOQ X X <l o q T NA 1<LOQ NA NA _<LQQ_ Na NA <LOO <LOQ <LOO n-a----------- N A 8 X X 19 NA N A 8 NA NA 12 N A N A <100 8 X A <LOO <LQQ . <LOO 11 8 <LO0 <LOO 8 X NA NA NA X 8 NA NA NA X NA NA NA X X NA NA NA m m m m m m m m m m m m m m m m m 3M Environm ental Lab - Fluorine A nalytical C hem istry Team Semi-quantitative results of the analysis of wild bird livers SAMPLE NUMBER SPECIES 4291001 44229911000098 ssaannddhhiillll ccrraannee sandhill crane 44229911002518 ssaannddhhiillll ccrraannee 444222999666000010849 44229966001187 ssssaaaannnnddddhhhhiiiillllllll ccccrrrraaaannnneeee sandhill crane 44333377000013 wwhhiittee ppeelliiccaann 44333377001074 4337018 wwwhhhiiittteee pppeeellliiicccaaannn 4306020 44330066002212 bbbrrraaannndddttt'''sss cccooorrrmmmooorrraaannnttt 44330066005516 bbrraannddtt''ss ccoorrmmoorraanntt 4444333311112222000000001562 4312007 double-crested cormorant double-crested cormorant dddooouuubbbllleee---cccrrreeesssttteeeddd cccooorrrmmmooorrraaannnttt 4360020 brown pelican 44336600002212 4360023 4360024 brown pelican brown pelican brown pelican brown pelican 4295007 4295008 44229955001147 4295018 sssaaannndddhhhiiillllll cccrrraaannneee sandhill crane sandhill crane 444333999222000000354 44339922000076 ddddoooouuuubbbblllleeee----ccccrrrreeeesssstttteeeedddd ccccoooorrrrmmmmoooorrrraaaannnntttt double-crested cormorant 44333366000036 44333366001102 4336013 bbrroowwnn ppeelliiccaann brown pelican bbrroowwnn ppeelliiccaann 4311003 4311005 444333111111000000679 (treat blue heron great blue heron greatblue heron great blue heron great blue heron 44332288005037 444333222886000555647 white pelican white pelican white pelican white pelican white pelican * A 444333777888000110135 44337788001146 venge when outlien arc bbrroowwnn ppeelliiccaann brown pelican ebbxrroocwlwunndeppdeelliiccaann LOQ " limit of quantitation (approx. 6 ppb) LOCATION KKKKKeeeeeaaaaamrmmmneeeeevvyvv.,.,, NNNNNEEEEE CCCoooccchhhiiissseee CCCooo...,,, AAAZZZ Cochise Co., AZ Cochise Co., AZ CCCaaallliiipppaaatttrrriiiaaa,,, CCCAAA Calipatria, CA Calipatria, CA SSaann DDiieeggoo.. CCAA San Diego. CA SSaann DDiieeggoo,, CCAA SL Martirrville, LA SSSSLttt MMMMaaaarrrrttttiiiirrrnrrrvvvviiiilllllllleeee,,,, LLLLAAAA Miami. FL MMMiiiaaammmiii... FFFLLL Miami, FL VVaalleenniiccaa CCoo..,, NNMM Socorro Co., NM Socorro Co., NM Valenica Co., NM Naples. FL Naples. FL Naples. FL NNaapplleess., FFLL Calipstria, CA Calipatria. CA CCaalliippaattrriiaa,, CCAA Calipatria, CA St Martinville, LA SSLL MMaarrttiinnvviillllee., LLAA S t Martirrville, LA S t Martinville, LA Fallon. NV FFFFaaaalllllllloooonnnn,,., NNNNVVVV F t Lauderdale, FL F t Lauderdale. FL FFF ttt LLLaaauuudddeeerrrdddaaallleee,,. FFFLLL MMSS-DS-aSnadnhdihllilCl rCarnaene Liver Liver Avg. Avg. [PFOS] ppb [PFOSA] ppb [PFOS] ppb [PFOS]ppb* 41 <LOQ ... <<LLOOQQ <l o q __ <LOQ <LOQ <<<LLLOOOQOO <<LLOOQQ <<LLQOQQ <-00 <LOQ <<<LLLOOOQQQ 35 1293 29 <g<LL'OOOQOQ <<<LLLOOOOQQ <-00 <LOQ 15 <LOO 155 <l o q 305 58 53 80 46 31 46 34 80 13 2055 94 456 56 59 20 145 7 333 23 76 170 <<ll o o q q 157 157 106 58 134 11 125 10 159 47 48 11 115 115 <<LLOOQO <<LLOOQQ <-OQ <LOQ <<lLoOqQ <LOQ <LOQ <LOQ <LOQ 212 9 10 <LOQ 52 <LOQ 100 35 152 24 105 105 16 36 <LOQ 6 <<L-O0 O0 <<LLOOOQ 32 <LOQ < 22 22 188 59 1061 261 173 <<L-118O029O0 348 348 141 < -0 0 392129637123 <<<L--OO0QQ0 <-OQ 371 371 17954 181 71 <LOQ 31 91 < -80 0 92 92 77% 64% 96% 64% Qualitataive results of the analysis ofwild bird livers SAMPLE Liver NUMBER SPECIES LOCATION [PFOS] ppb 4291001 sandhill crane Kearney, NE 41 4291008 4291009 4291021 4291038 4296008 4296009 4296014 4296017 4296018 4337001 sandhill crane sandhill crane sandhill crane sandhill crane sandhill crane sandhill crane sandhill crane sandhill crane sandhill crane white pelican Kearney, NE Kearney, NE Kearney, NE Kearney, NE Cochise Co., AZ Cochise Co., AZ Cochise Co., AZ Cochise Co., AZ Coohise Co., AZ Calipatria, CA <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <LOQ <L35OQ 4337003 4337004 4337017 4337018 4306020 4306021 4306022 4306031 4306036 4312001 4312002 4312003 44331122000067 4360020 white pelican white pelican white pelioan white pelican branch's cormorant branch's cormorant branch's cormorant branch's cormorant branch's cormorant double-crested cormorant double-crested cormorant double-crested cormorant double-crested oormorant double-crested cormorant brown pelican Calipatria, CA Calipatria, CA Calipatria, CA Calipatria, CA San Diego. CA San Diego, CA San Diego, CA San Diego, CA San Diego, CA S t Martinville, LA S t Martinville, LA S t Martinville, LA S t Martinville, LA S t Martinville, LA Miami, FL 1358 30 17 167 57 4s6o 85 2176 61 149 336 78 178 115 4360021 4360022 4360023 4360024 4295007 4295008 4295017 429S018 4293014 4392003 4392004 4392005 4392006 4392007 4336003 4336006 4336010 4336012 4336013 4311003 4311005 4311006 4311007 4311009 4328007 4328053 4328054 4328056 4326057 4378005 4378011 4378013 44337788001164 brown pelican brown pelican brown pelioan bsaronwdhnilpleclriaonaen sandhill crane sandhill crane sandhill crane sandhill crane double-crested cormorant double-crested cormorant double-crested cormorant double-crested cormorant double-crested cormorant brown pelican brown pelican brown pelican brown pelican brown pelioan (treat blue heron (treat blue heron (treat blue heron (treat blue heron (treatblue heron white pelican white pelican white pelican white pelioan white pelioan brown pelican brown pelican brown pelican brown pelioan brown pelican Miami FL Miami, FL Miami. FL Miami FL Valenica Co., NM Valenica Co., NM Socorro Co., NM Valenica Co., NM Socorro Co., NM Naples. FL Naples, FL Naples, FL Naples, FL Naples, FL Calipatria, CA Calipatria, CA Calipatria, CA Calipatria, CA Calipatria, CA S t Martinville. LA S t Martinville. LA S t Martinville. LA S t Martinville, LA S t Martinville, LA Fallon, NV Fallon, NV Fallon, NV Fallon, NV Fallon. NV F t Lauderdale, FL F t Lauderdale, FL F t Lauderdale. FL F t Lauderdale, FL F t Lauderdale, FL 149 126 166 49 11 9 <LOQ <LOQ <LOQ 226 11 54 103 154 16 37 <LOQ 7 33 202 61 1143 274 178 146 368 941 141 294 212 76 72 32 94 X **detected ** ~ not detected PFOSAA, M556, M570, POAA, and M513 were not detected in the Ihrere of any bird. PFHS7 PFDS? X * not measured not measured ** ** not measured not measured not measured not measured * X * not measured not measured not measured X X not measured not measured not measured X not measured not measured not measured X X not measured not measured not measured ** not measured not measured not measured X X X not measured notmessured not measured X X ** not measured not measured not measured X notmessured not measured not measured ** ' X * X not measured not measured not measured notmessured not measured notmessured * ** ** ** not measured not measured not measured not measured not measured not measured ** X ** ** not measured notmessured not measured not measured not measured not measured XX ** X not measured notmessured not measured not measured not measured notmessured ** X ** X not measured not measured not measured notmessured not measured notmessured ** X XX not measured notmessured not measured notmessured not measured not measured XX ## X nnoott mmeeaassuurreedd notmessured notmessured notmessured notmessured 3M Environmental Lab - Fluorine Analytical Chemistry Team PFS's PFHS perfluorohexanesulfonate C6F13S03- PFOS perfluorooctanesulfonate C8F17S03- PFOSA C8F17S02NH2 PFOSAA C 8F 17S 0 2 N ((C H 2 C H 3 )(C H 2 C 0 0 ))- M556 C8F17S02N((H)(CH2C00))- M570 C8F17S02N((CH3)(CH2C00))- PFOSEA perfluorooctanesulfonylethyl amide C8F17S02N((CH2CH3)(H)) Et-FOSE-OH PFDS perfluorodecanesulfonate CC180FF1271SS0O2N3-(*(C* H2CH3)(CH2CH20H)) P F C A 's POAA M513 M613 M463 M563 M363 perfluorooctanoate perfluorodecanoate perfluorododecanoate C7F15COOC9F19COOC11F23COOC8F17COOC10F21COOC6F13COO- PFS = perfluorinated sulfoante PFCA = perfluorinated carboxylic acid ** no standard material available for confirmation; 000529