Document QXXOvOoQKk8wjvLVDE4kBkORo

Afc-o1 ACTIVATED SLUDGE RESPIRATION INHIBITION TEST SUBSTANCE Identity: Perfluorooctanesulfonylamido(ethyl)acetate; may also be referred to as PFOSAA, FC-128 or as the major component of FC-127, FC-129, FC-109, FC-109-X or R1904. (Glycine, Nethyl-N-[(heptadecafluorooctyl)sulfonyl]-, potassium salt, CAS # 2991-51-7) Remarks: The test sample is FC-129 referred to by the test laboratory as R1904. Current information indicates it is a mixture of 42% test substance, 32% water, 14% 2-butoxyethanol, 4% ethanol, and 8% C4-C7 fluoroalkyl carboxylate compounds. Test material was from 3M production lot 930014, mfg. date 3/97. The following summary is abbreviated because the test sample is a mixture of the test substance in a water/organic solvent solution. Data may not accurately relate toxicity of the test sample with that of the test substance. METHOD: Methods: OECD 209 Test type: Static acute GLP: No Year Completed: 1997 Test organism source: Activated sludge collected from the City of Superior, Wisconsin, Wastewater Treatment Plant. RESULTS Nominal concentrations: Bk control, Reference substance, 3.75, 7.5, 15.0, 30 mg/L Element values: 30-minute EC50 = >1000 mg/L 3-hour EC50 = >1000 mg/L (No inhibition exhibited) All element values based on nominal concentrations. Remarks: Testing was conducted on the mixture of the test substance as described in the test substance remarks field. The value reported applies to that mixture and not the test substance. No attempt was made to determine the impact of the presence of the organic solvents or what portion of the toxicity can be contributed to the Perfluorooctanesulfonylamido(ethyl)acetate. CONCLUSIONS The FC-129 3-hour EC50 for activated sludge respiration inhibition was determined to be >1000 mg/L. Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY ______________ Reliability: Klimisch ranking 2. Testing meets all criteria for quality testing, but lacks analytical confirmation of test substance concentrations. There is a lack of characterization of the test sample. REFERENCES This study was conducted at AScI Corporation of Duluth, Minnesota at the request of the 3M Company. OTHER Last changed: 5/17/00 000412 AScI Corporation/AScI-Duluth Environmental Testing Division AScI Study ID# 5010-068 STUDY TITLE INHIBITION OF RI904 FOR ACTIVATED SLUDGE RESPIRATION DATA STANDARD OECD GUIDELINE 209 STUDY COMPLETED JULY 10, 1997 TESTING FACILITY AScI Corporation AScI-Duluth Environmental Testing Division 4444 Airpark Boulevard Duluth, MN 55811 Tel. No. (218) 722-4040 PROJECT IDENTIFICATION NUMBERS AScI Study ID# 5010-068 3M Company Lab Request# R1904 Sponsor: 3M Company Sponsor Study ID# R1904 *1 000413 AScI Corporation/AScI-Duluth Environmental Testing Division AScI Study ID# 5010-068 1.0 GENERAL INFORMATION Study Title Data Standard Sponsor Sponsor's Representative Sponsor's Lab Request# Testing Facility Principal Investigator Project Director Testing Facility Director Definitive Test Date Data Validation and Report Review Inhibition of R1904 For Activated Sludge Respiration OECD Guideline 209. 3M Environmental Technology and Safety Services, Building 2-3E-09, 935 Bush Avenue, St. Paul, MN 55133; Tel No. (612) 778-7452. Susan Beach, 3M Environmental Technology and Safety Services, Building 2-3E-09, 935 Bush Avenue, St. Paul, MN 55133; Tel No. (612) 778-7452. R1904 AScI Corporation/AScI-Duluth Environmental Testing Division, 4444 Airpark Boulevard, Duluth, MN 55811; Tel. No. (218) 722-4040. Joe Dierkes Joe Amato Donald Mount July 1, 1997 Alan Mozol Retention of Raw Data and Final Report Location of Raw Data and Final Report Five years from the date study is completed. AScI Corporation/AScI-Duluth Environmental Testing Division, 4444 Airpark Boulevard, Duluth, MN 55811; Tel. No. (218) 722-4040. 2.0 OBJECTIVE To determine if possible, the 30-minute and 3-h (contact times) EC50's of the test substance R1904 for activated sludge respiration. This study was conducted according to the AScI study plan retained by the Sponsor and OECD Guideline 209 (OECD 1993). Sponsor: 3M Company Sponsor Study ID# R1904 2 000414 AScI Corporation/AScI-Duluth Environmental Testing Division AScI Study ID# 5010-068 3.0 TEST METHODS Test Substance Properties: R1904, yellow liquid, specific gravity ca. 1.3 (water = 1), stable, and completely soluble in water. Reference Substance Test concentrations: 0 and 1,000 mg/L prepared in duplicate by direct additions. Name: 3,5-dichlorophenol, purity 97%, Aldrich Chemicals, Lot# 02110JX. Test Organisms Test concentrations: 3.75, 7.5, 15.0, and 30 mg/L prepared by direct addition of 500 mg/L stock solution in Millipore water. Source: Activated sludge from the City of Superior (Wisconsin) Wastewater Treatment Plant receiving approximately 95 % domestic sewage. Synthetic Sewage Feed Use in test: (1) Centrifuged five minutes, three times (4C, 7500 rpm), (2) diluted the sludge pellet with carbon-treated water to selected volume, final total solids concentration of 1.6 g/L was obtained by further dilution, and (3) added 50 ml of synthetic sewage feed per L of the diluted sludge, and aerated at 20C until test initiation. The sludge was held for less than 24 hours between collection and test initiation. Composition: Chemical g dissolved/L of deionized water Deionized Water Bacto. peptone Beef extract Urea NaCl CaCl2.2H20 M g S 0 4.7H20 K2H P04 16.0 11.0 3.0 0.7 0.4 0.2 2.8 Deionized water used in the study was obtained from a Millipore Milli-Q System. The most recent chemical analysis is in Raw Data Package. Sponsor: 3M Company Sponsor Study ID# R1904 3 000415 AScI Corporation/AScI-Duluth Environmental Testing Division AScI Study ID# 5010-068 Test Setup Determination of Respiration Calculation of Respiration Rate Endpoint Calculation Exposure vessels: 8, 1-L borosilicate glass beakers; two inoculum controls, two test exposures, and four reference substance treatments. Order o fpreparation: At 10-minute time intervals: (1) first inoculum control, (2) test exposure concentration replicates, (3) from low to high reference substance exposure concentrations, and (4) second inoculum control. The final volume in each beaker was 800 ml. Preparation: (1) Added 284 ml of the sludge preparation in each beaker, (2) added 26 ml of synthetic sewage feed to each beaker, and (3) in test and reference exposures, added the required amounts of the test and reference substances, respectively, (4) and diluted each exposure to 800 ml with carbon-treated water. Incubation and aeration: 19.9 to 20.0C and vigorous aeration. Sequence: Same as exposures were prepared. Determination: At 30-min and 3-h contact times: (1) filled one 300ml BOD bottle with the exposure medium and continued to aerate the remaining medium, (2) using a YSI 54A oxygen meter with a self stirring probe, determined the DO concentration in the exposure medium every 0.5 minutes for up to 5.5 minutes (the first determination was made after 0.5 minutes), and (3) the sub-sample was properly disposed. DO meter calibration checks were made between the sets of measurements for each control or treatment. For each exposure, correlated DO depletion and time interval, and calculated the slope of the regression line as the respiration rate. EC50: Probit Analysis (EPA 1988). Sponsor: 3M Company Sponsor Study ID# R1904 000416 4 3.0 RESULTS Test Substance Toxicity (Pertinent data are in Table 1) Reference Substance Toxicity (Pertinent data are in Table 1) 30-minute EC50: > 1,000 mg/L. 3-hE C S0: >1,000 mg/L. 30-minute EC50: <3.75 mg/L. 3-h ECS0: <3.75 mg/L. AScI Corporation/AScI-Duluth Environmental Testing Division AScI Study ID# 5010-068 4.0 TEST QA/QC QA/QC Criteria Respiration rates of two inoculum controls must not vary by more than 15 %. 3-h EC50 of reference substance must be between 5-30 mg/L. Test duration must be 3 h. Data Variations after 30-minute and 3-h contact times were less than 3%. 3-h EC50 was <3.75 mg/L indicating the sludge had greater than normal sensitivity. The lack of effect due to R1904 is a valid result. The test duration was 3 h. 5.0 REFERENCES Organization for Economic Cooperation and Development (OECD). 1993. OECD Guidelines for Testing of Chemicals. OECD Publication Information Center, Washington, D.C. U.S. Environmental Protection Agency (EPA). 1988. Computer Program Probit. Biological Methods Branch, EMSL, U.S. EPA, Cincinnati, OH 45268. Sponsor: 3M Company Sponsor Study ID# R1904 000417 5 TABLE 1.0 OECD ACTIVATED SLUDGE RESPIRATION INHIBITION TEST #209 CALCULATION OF RESULTS Test Substance: R1904 stdy N 5010-068 Date: 7\1\97 Analyst: DIERKES 30-Minute Exposure________________ Dissolved Oxygen Concentrations, mg/L mg/L 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 5.0 5.5 Temp, *C R (1) Ra (2) % In h . (3 ) BK1 6.8 6.5 6.0 5.6 5.1 4.6 4.2 3.7 3.2 2.7 2.2 1000A 6.9 6.5 6.2 5.8 5.4 5.0 4.6 4.2 3.8 3.3 3.0 1000B 6.8 6.4 6.0 5.6 5.2 4.8 4.4 4.0 3.6 3.2 2.8 3.75ref 7.6 7.3 7.1 6.9 6.6 6.4 6.2 5.9 5.7 5.5 5.2 7.5ref 7.6 7.4 7.3 7.1 7.0 6.8 6.6 6.4 6.2 6.1 5.9 15ref 8.1 8.0 7.9 7.8 7.8 7.7 7.6 7.5 7.4 7.3 7.3 30ref 8.7 8.6 8.6 8.5 8.5 8.4 8.4 8.3 8.2 8.2 8.1 BK2 6.9 6.4 6.0 5.5 5.1 4.5 4.1 3.7 3.1 2.6 2.2 % diff in controls(4) 1.0 ECS0 195% Ch: >1000 ma/L (NC) 19.9 19.9 20.0 19.9 20.0 19.9 20.0 20.0 0.9503 0.9569 0.7976 0.8031 0.8000 0.8000 0.4655 0.4687 0.3442 0.3442 0.1636 0.1648 0.1139 0.1139 0.9479 0.9479 RaCm (5): 16 16 51 64 83 88.0 0.9524 3-Hour Exposure___________________ Dissolved Oxygen Concentrations, mg/L mg/L 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 5.0 5.5 Temp, *C R (1) Ra(2) % In h . (3 ) BK1 7.5 7.0 6.7 6.2 5.8 5.4 5.0 4.6 4.1 3.7 3.2 1000A 7.3 6.8 6.4 5.9 5.5 5.1 4.6 4.2 3.7 3.2 2.8 1000B 7.0 6.6 6.2 5.7 5.3 4.8 4.3 3.9 3.4 2.9 2.5 3.75ref 8.3 8.1 7.9 7.7 7.5 7.3 7.1 6.9 6.8 6.5 6.1 7.5ref 8.5 8.3 8.2 8.0 7.9 7.7 7.6 7.4 7.3 7.2 7.0 15ref 8.9 8.8 8.7 8.6 8.6 8.5 8.4 8.4 8.3 8.3 8.2 30ref 9.0 9.0 8.9 8.9 8.9 8.8 8.8 8.8 8.7 8.7 8.7 BK2 7.9 7.4 7.0 6.6 6.2 5.7 5.3 4.9 4.4 4.0 3.5 % diff in controls(4) 2.4 EC 50 (95% Cl): >1000 ma/L (NC) 20.0 20.0 19.9 20.0 19.9 20.0 20.0 20.0 0.8448 0.8448 0.8945 0.8945 0.9236 0.9301 0.4158 0.4158 0.2885 0.2905 0.1261 0.1261 0.0655 0.0655 0.8655 0.8655 RaCm (5): -5 -9 51 66 85 92.3 0.8552 (1 ) R= Oxygen uptake rate (mg 02/L -m in ) (2 ) R a R adjusted to 20*C = [R /e.E x] (3 ) % Inhibition [(R aC m )-R aS/(R aC m )]100 (4 ) % difference in controls: [IR aB k1-R aB k2l/R aC m ]100 (Tw o controls must be within 15% of each other.) (5 ) M ean adjusted R , controls [R aB k1+RaB k2]/2 0= 1.072 RaC m m ean adjusted R , controls Bk blank control, S sam ple Tobs sam ple tem p x= Tobs-20*C AScI Corporation/AScI-Duluth Environmental Testing Division AScI Study ID# 5010-068 Sponsor: 3M Company Sponsor Study ID# R1904 000418