Document QXVJmnO1rD4p1d7xdnYmMyqyL

ACUTE TOXICITY TO FISH (FATHEAD MINNOW) TEST SUBSTANCE Identity:A mixturecontainingperfluorooctanesulfonatweh,ich may alsobe referredtoas PFOS, FC-95, or as a component of FC-3017. (1Octanesulfoniaccid)(CAS # 2795-39-3). Remarks: The 3M productionlotnumber was not noted. The testsample isFC,3017. CurrentinformationindicatesFC-3017 isa mixtureof0.67% PFOS, 20% diethyleneglycolbutylether,55.41% water,21 % ethyleneglycol, 1.33% Hydroxy foamer, 1% sodium octylsulfate0,.5% polyoxyethylene monooctylphenylether,0.04% sodium laurylsulfatea,nd 0.05% tolyltraizole. The followingsummary applies to a mixture with incompletely characteilzedconcentrations of impurities.Data may not accurately reflecttoxicityof the fluorochemical component of the testsample. METHOD: Method: OECD Guideline203 Type: Acute static-renewal GLP: No Year completed: 1995 Species: Pimephalespromelas Supplier:Aquatc Biosystems,Inc.,FortCollinsC,O. Analyticalmonitoring:Temperature,pH, and DO. Exposure period: 96-hours Statisticamlethods: Not given. Test fishage: Juveniles(121- 132 days) Length and weight: Average length 23 mm Average weight 0.0908g (wet) Loading: 0.504g/L Pretreatment:None Test conditions: Dilutionwater: Carbon-filterweedllwater Dilutionwater chemistry: DissolvedOxygen: 8.4mg/L pH: 8.4 Lighting:Not given. Stock and testsolutionpreparation:The testmateriawlas added directly tothe dilutiownater on a weighvvolume basisand mixed wellwitha glass rod. Concentrations dosing rate: Once forthe control,1.0 and 10 mg/L exposures. Twice forthe 100 mg/L exposure,and threefiniesforthe 1000 mg/L exposure. Stabilityof the testchemical solutions: Not noted. Exposure vessels: 2.0L beakers containing1.8L testsolution. Number ofreplicates1:for1,10,and 100mg/Lexposuresc,ontroalnd 1000 mg/L exposure were duplicate. Number of fishper replicate:10. Number of concentrations: fourplusa blankcontrol Water chemistry during the study: PH range (0-96hours): 8.0- 8.4 (controlexposure) 7.7- 8.4 (1000 nig/Lexposure) Temperature range (0-96 houm): 17.8- 19.9 OC Dissolved oxygen mnge (0-96 houm): 6.5 - 8.4 mg/L (controlexposure) 0.7 - 8.4 rng/L(1000 mg/L exposure) RESULTS Nominal concentrations: Blank control,1, 10, 100, and 1000 mg/L Element values: 96-hour LCm = >1 000 nig/L Element values based on nominal concentrations Remarks: Tesfing was conducted on the mixtureas described in the Test Substance Remarks field.The values reportedapply to thatmixtureand not the fluorochemicalproportionalone. CONCLUSIONS The testsample 96-hour LC50 forfathead minnow was determined to be >1000 mg/L, the highestconcentrationtested.No mortalitywas observed at thisconcentration. Submitter 3M Company, Environmental Laboratory,P.O. Box 33331, St. Paul,Minnesota, 55133 DATA QUALITY Reliability:Klimischranking = 3. Testing approach inconsistentamong exposure concentrations,with a combinafion of staticand static-renewal techniques used. Sample puritywas not propedy charactedzed and the study lacksanalyticaclonfirmationofthe amount of fluorochemicalproportioninthe solution. REFERENCES This study was conducted by the 3M Company, Environmental Laboratory, St.Paul,MN, Lab Request number N3188,1995. OTHER Last changed: 6/26/00 Sample Desc.-ip*,ion Environmenlal Lab - Work Sheet Lat) Re@zues, Page Date: ot 7/ Analys*,: 4e_ Ile c, e -T T I w--F Ile @Z @,-i i C) C-)n C) tjC,,oS ri;-+t\t@ t,@ @a c L-@ea L, et-. -Pet;- Lt-c vi-, f-c- LL-@ I ki@6 cli Ck )U e.&,\jI @-cevt D c ry- I/ c -@ile_C,Ir-,- <'cLtrL-,,4, v c% Fr 4E .1 1 f- a C C,% i v iA ii L,),-4er L&-"CsCiLdieci @n 4((?I)il I)eckLe[s-6L e I 'S OA tpA eZ tS ci ti1-0 1 e t3L o<1 4-c (? c@C C! Ct 1'3 C- /,,_I cl, (@e rcli 9 e ti ke?l- 41 A t/ /0/'Y e(I 6 k,2 t.-@ r a, Q 5 @e I- AA- 4-lkpb v ("k s !s e t f c, Lc .2 c S C)6 L @e C4 Le s 12KW'tA@(Ik' lu@iclo fl?O Ie -in. @T@iu '166(') cm,@ IL c S A A!i 9 /2:g Iq y] D.'JC) A't @?,q.T).O, 3.7@4 IL c ItA4@- Ce,k t@6 '/A) I:t (L)6 Sample Description i-@le C Environmental Lab - Work Sheet La-- :-ie-ues'N,C. Page cf @7 Date: A.-.alys*: i'CL 4,) e c C- 0 it, C ez c /7 ol 7c i ENVIRONMENTAL LABORATORY LR N3188 TYPE TEST: 96-HR ACUTE TO)OCITY TO FATHEAD MINNOW, RUEPHAI-ES PROMELAS VI@y STATIC --X- STATIC RENE WAL TEST SUBSTANCE: FC,3017 DILUTION WATER: CARBON-FILTERED WELL WATER (3M WELL #2,ST. PAUL, MN) Temp. 1999 C.pH 8.4 . 0.0. 8.4 mg/L TEST FISH SOURCE: AOUA71C BIOSYSTEMS, INr- FORT COLLINS, CO. FISH AGE: 121-132days "G. WT.: , @'i DATE RECEIVED. 7/26/1995 "G. SIZE-- NO. OF FISWTEST CHAMBER: 10 LOADING RATE: L TEST SOLUTION VOLUME: 1.8 L SIZE OF TEST VESSELS: 2.0 L BEAKER DATE INITIATED: 9/25/1995 DATE TERMINATED, 12411 Z--i TEST INITIAL TOTAL VESSEL CONC. AMOUN1 24-HR pH mgA. ADDED* SKI CONTROL BK2 CONTROL c I-A 1 1.8 D.O. 48-HR mg/L. MORTALITY pH 0.0. 72-HR pH mg/L i D.O. WHR nvg& pH 0.0. mgAL I-B 10 18 I-C 100 180 I-D IOOD 18M 2-D 1000 18W 7 (S.C@ c S'@ -7 C -3 -7 r -.2 2,q C 2-, 2 1 TEMP. *C IDATE / ANALYST C,le-T -7 LCSO (95% C.I.) ICCC, OOM M ENTS -INDIVIDUAL WEIGHTS, mgA-. Tj, :r L@ L c SECONDARY -,rTi.@Ine- IL- r-e&;,@,ett ork@'4.t.--7L2c-'..Mae ea'*t ft,ti#-4eLi EFFECTS: S SwMc".-t Loss ofEquii'-Aum;0 OWescont R a EffatiScwimrring-H. Hemorrhaglr4 ANALYST.- Environmental Laboratory LR Date 96-Hour Acute ToxicityTo Fathead Minnow, Plmephales Promelas Loading Rate Data Sheet Test Substance- @)C)1-7 Minnow 1 2 3 4 5 6 7 8 Weight, (q)-Length. (cm) -- C, 12'--1', o (i@n c 1,@@.,!s (-1 c@-c @-qC@- 10 Mean Avg. c.o <1og 2 Loading Rate Calculation Mean Avg. wt.,(g)C.(,5C@x'lO=-L.@-c-S g. (n) g/l.OL =C-,ciC,cg3/l.BL (n) g= C,.!SC)L4 Loading Rate= g/L.