Document QQ0J8QDJbbRY9db7M0MOXpk

X flcaranun 7-1V A TECHNICAL REPORT SUMMARY "&/1 6 /7 8 TO: TECHNICAL COMMUNICATIONS CENTER 201-2CN .im p o rta n t - if report is printed on both sides o f paper, send two copies to T C C J D,U,S,rRT, - Analytical and Properties Research Laboratory O ept. N um b er 0502 P ro je ct Trace Fluorochemicals in the Environment-------- _-- ------- R eport T itle , IRDC 137-088: FM-3422/Monkey A000006 995 To J.E. Long ..... .-- -------- -| A u th o r(s) Jon Belisle_____ _____________ _____________________-------- N otebook R eference 88240 3 SECURITY C] Open C-k Closed 3M CH EM ICAL w; REGISTRY w New Chemicals Reported Ye* C X No KEYWORDS: (Select terms from 3M Thesaurus. Suggest other applicable terms.) CURRENT OBJECTIVE: Requestor:J.E. Long; Request #A69503; Project #9172110004 Central Research Analytical Repor Chemical/Analysii R EPO RT A B ST R A C T: (200-250 words! This abstract information is distributed by the Technical Communications Canter to alert 3M'ers to Company R&D. It is Company confidential material. CLOSED ABSTRACT ~" The level of FM-3422 was determined in the serum and livers of monkeys from the IRDC subacute study 137-088. Furthermore total fluorine content was determined in selected serum samples and the metabolism of FM--3422 characterized by F/NMR. In form atio n Liaison Soi 11..It pwO C E N T R A L A N A L Y T IC A L LABORATORY R eport No. 6995 D ate August 1 6 , 1978 ibject: IRDC 1 3 7 - 0 8 8 equestor: __i s.1 - . equest No. .^6 950 3-------- eport : FM-3422/Monkey Dept. N a m e _.Toxicology__ D ated _ _ - -------------------- Proj. No. 972.110.004 ^ i nftfl bv IRDC was a 90 day subacute Rhesus monkey toxicity R!ieS r Fi " 422 ^The^original study was set at the following dosage study of F 1000 mg/kg/day. The study was revised with levels: 30, 300 mg/kg/day. It appears (see Table bSlSw? t h a t 1 thefdosage levels were lowered again prior to the study. sirSi and livers were each individually submitted for analysis. Each group contained 4 monkeys, 2 male and 2 female as follows. Group I II III IV V Dosage Level 0 1 3 mg/kg/day 11 10 II 30 II Survival 4/4 4/4 4/4 4/4 3/4 SERUM ANALYSIS Monkey^ Dosage(2) 7470M 0 7493F 0 FM-3422 in Serum (ppm) 0 0 Total F in Serum (m) nm nm 7467M 7489F 1 1 0 0 nm nm 7469M 74 74M 7487F 3 3 3 0.1 nm 0.1 nm 75 nm v.U. #6995 Mcnk,eyQj 7475M 7498F Dosage*^ 10 10 7472M 7499F 30 30 2- - FM-3422 in Serum (ppm) nm 0.4 August 16, 1978 * Total F in Serum (ppm) 150 nm 0.5 375 0.7 nm pp Q-l = Male, F = Female n mg/kg/day m = not measured The analysis of the serum for FM-3422 indicated that this compound was present at a very low concentration. Total fluoride analysis indicated the presence of a significant level of fluoride. Therefore the serum of 7472M was prepared for F/NMR analysis. The NMR spectra indicated (Richard Newmark) the presence of. FC-95 with no other significant fluorine peak noted. A very high branched content was observed (80% branched/20% linear). The branched/linear ratio of FM-3422 used in the feeding study was found to be 40% branched/60% linear. Perhaps the branched isomer is retained to a greater extent than the linear. The fact that a high level of FC-95 was found in the serum and essentially no FM-3422, indicates that FM-3422 is metabolized to the sulfonic acid. Furthermore, the study would indicate that FM-3422 is cleared from the body rather rapidly, that is before it is completely converted to FC-95 since the monkey tolerates FM-3422 at concentrations approximately 10 times greater than FC-95; this suggests that FC-95 builds up slowly with time. LIVER ANALYSIS Monkey Dosage^ FM-3422 in Liver(yg) FM-3422 in Liver(ppm) 7469M 7487F 3 3 110 190 1.3 2.6 7472M 7499F 30 30 0 in mg/kg/day 1300 4600 14.3 59.2 A.U. #6995 3 August 16, 1978 The high values of FM-3422 found in the liver when compared to the low level in the blood may indicate conversion of FM-3422 to the sulfonic acid (see above F/NMR data on serum)in the liver. The data shows that the female accumulates and/or retains FM-3422 at a level 2 to 3 times higher than the male. In addition, the only animal to die during the study was a female, perhaps again suggesting a greater sensitivity of the female to this compound. n Belisle /jz