Document QMmLo96p2M1zqmpJJoJmerzE4

AA 6 - Oi3^ ACUTE TOXICITY TO FISH (FATHEAD MINNOW) TEST SUBSTANCE____________________________________________ Identity: A mixture containing perfluorooctanesulfonate, which may also be referred to as PFOS, FC-95, or as a component of FC-600. (1-Octanesulfonic acid, 1,1,2,2,3,3,4,4,5,5,6,6,7,7,8,8,8heptadecafluoro-, potassium salt, CAS # 2795-39-3) Remarks: The 3M production lot number was 2367. The test sample is FC-600 (Belgium). Current information indicates it is a mixture of 1.0% PFOS, 81.20% water, 12.00% diethylene glycol butyl ether, 1.00% sodium octyl sulfate, 2.00% propane Sultone foamer, 1.00% sodium decyl sulfate, 0.85% xanthan gum, 0.1% N-(3-chloroallyl) hexaminium chloride, 0.80% starch, and 0.05 % tolyltriazole. The following summary applies to a mixture with incompletely characterized concentration of impurities. Data may not accurately reflect the toxicity of the fluorochemical component of the test sample. METHOD:___________________________________________________ Method: Not noted. Test type: Acute static (Range-finding) GLP: No Year Completed: 1983 Species: Pimephales promelas Supplier: Commercial supplier in Brady, Nebraska Analytical monitoring: Temperature, pH, DO Exposure period: 96-hours Statistical methods: Not applicable. No mortality at highest concentration tested. Test fish age: Juvenile Average Length and weight: Length = 3.6 cm Weight = 0.39 grams Loading: 0.65 g/L Pretreatment: Not noted. Test conditions: Dilution water: Carbon-filtered well water, St. Paul, MN. Dilution water chemistry: pH: 8.2 Dissolved Oxygen: 9.6 mg/L Temperature range: 18C Lighting: Not noted 002028 Stock and test solution preparation: Test solutions were created by direct weights additions. Concentrations dosing rate: Once Stability of the test chemical solution: Not noted Exposure vessels: Wide-mouth one gallon glass jars containing 3 liters of test solution. Number of replicates: One Number offish per replicate: 5 Number of concentrations: Four plus two blank controls Water chemistry during the study: pH range (0 - 96 hours): 8.0 - 8.2 (control exposures) 7.7 - 8.2 (1,000 mg/L exposure) Dissolved Oxygen range (0 - 96 hours): 5.8 - 9.6 mg/L (control exposures^ 1.7 - 9.6 mg/L (1,000 mg/L exposure) Temperature range (0 - 96 hours): 18 - 21C RESULTS___________________________________________________ Nominal concentrations: 2 blank controls, 1,10,100, and 1,000 mg/L Element value: 96-hour LC5o= >1,000 mg/L Element value based on nominal concentrations. Remarks: Testing was conducted on the mixture as described in the Test Substance Remarks field. The value reported applies to that mixture and not the fluorochemical proportion alone. CONCLUSIONS______________________________________________ The FC-600 96-hour LC5ofor Pimephales promelas was determined to be >1,000 mg/L. Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY______________________________________________ Reliability: Klimisch ranking 2. The study meets all the criteria for quality testing. However, the sample purity was not properly characterized and the study lacks analytical confirmation of the amount of fluorochemical proportion in the solutions. 002029 REFERENCES This study was conducted by 3M Company, Environmental Laboratory, St. Paul, MN, Lab Request number 8875,1983. OTHER____________ Last changed: 6/28/00 BEST COPY AVAILABLE ENVIRONMENTAL LABORATORY AOUATIC TOXICITY DATA SHEET EXPLORATORY (RANGE-FINDING TEST) Type Test Material Tested LR f f t 'jT' AvS- w t- Source^ . grams Avg. S i zqJ ^ ^ C M No . Organisms per dose T est S o lu tio n Volume 3 L ite r s /A ll-G la ss Tank Tank Dimensions:WIDE-MOUTH 1 GALLON GLASS JAR Time Started '*/& $ / ^ D a t e S tarted Z /fa Date Completed Z / f C i M ^ D ilu tio n Water C a rb o n -filtered w e ll w ater (Temp. D.O. ppm. pH /? ,Z ^ (P u t ^ --------- " so (9S* C & ^ c . L i . l t s ) t i :-- iV Cone. _ * & / ; _______ total Amount Added C Control - 1 _ J ______ i _ L l __ - ^ Q . . \o o .s o n - 4 ,_ \,0 0 Q S o o n 24 1Hours Total D.O. No. Dead pH ppm 0 fir/ 0 a/ n. n fa t If o (e ff 48 flours -- t t t j ours 96 Hou rs Total D.O. Total D.O. T otal D.O. No.Dead pH PP No.Dead pH ppm No.Dead >H ppm n f,( //* S 0 h 0 i 10 0 l yy 0 Ff.f M - - - - - --1 \4 V A / f l 1 k O n i tT f,! O f t M tf :f t \ ( n O v a fa O 0 C SSL n \ ]( 0 t v /,* / V Z i* } 0 f f ZxL-, T est Temp. C. - I n L U . f l . l a g Date Comments ______ ........ s s r a ? / , U K ri^ H ts,. .n a '/ y X c - V ' X/A fA fl. E ffects other than M ortality EC50- Tank No. Symptoms or R eactions 24-Hr. Total No. A ffected 48 Hr. 72-Hr. Control f^kOvtvl -V -l cfJrZl. 4-^ n TC M * . /ruffC:. A/riJJCZ A/AJ.Py A /zj<> /lfiC4r/ A /fctP ',.. . 44- ______ 1 M m .1___ -S _ ______ 96- Ht . / ( 'J . - - . . A/fM/f?_____ ______ A /oaj 002081