Document QJaLvbYE8K34L8pg51e0KJwv7

: AR 126-0090 E-S AMCUUSTSEELT)OXICITY TO AQUATIC INVERTEBRATES (FRESHWATER TEST SUBSTANCE Identity: FPeCr-f9i5u.or(o1o-cOtacnteasnuelsfuolnfaotnei;c amcaidy,a1l,s1o,2b,e2,r3e,f3e,r4r,e4d,5t,o5,a6s,6P,7F,O7,S8.o8r,8- heptadecafluoro-, potassium salt, CAS # 2795-39-3) Remarks: The test substance is a white powder. Sample was taken from 3M lot number 217. Samp testing. Purity determined le to was stored be 90.49% un by der ambient condi LC/MS, 'H-HMR, tions "*F- prior to NMR and elemental analyses techniques. METHOD Method: The study was conducted using a protocol based on procedures outlined in U.S. Environmental Protection Agency Series 850 -- Ecological ETfofxeicctistyGTueisdte;lianneds,AOSPTPMTSStaNnudmabrderE782590-.8180a7,5 S; tOanEdCarDd2G0u3i:dFeisfho,r Acute Conducting Toxicity Tests with Fishes, Macroinvertebrates and Amphibians. GTeLsPt:tyYpees: Semi-static Renewal Year Completed: Study completed 1999. Species: Unio complamatus Report completed 2000 Analytical monitoring: Test substance concentrations measured by LCMS at 0, 48, 96-hours Statistical methods: LCs values calculated, when possible, by probit analysis, moving average method or binomial probability with non-linear Tinetsetrpoorlagtainonisumsisnogutrhcee:comOpbuttaeirnesdofftrwoamreCaorfolC.iEn.a BSitoelpohgaicna.l Supply `Company, Burlington, North Carolina. Carolina collected from the wild. Test organism age at study initiation: Unknown Test conditions DDiilluuttiioonn wwaatteerr:c0h.e4m5iustmryfil(tdeurerdinwgeltlhwea4te-rweek period immediately preceding the test): hardness: 126 (120-132) mg/L as CaCOs alkalinity: 174 (170-178) mg/L as CaCO3 TPHo:c: <81.30(8m.g1-l8.5) Conductivity: 321 (310-330) umhos/cm CalMg ratio: 35/135 000501 Na/K ratio: 21.3/6.62 Lighting: Colortone 50 fluorescent lights, intensity approximately 369 lux. Photoperiod of 16-hours light, 8-hours dark with a 30minute transition period. Stock and test solutions preparation: A primary stock solution was prepared in dilution approximately 24 hours water at 1 prior to use. mg/L. After It was mixed for mixing, the primary stock was proportionally diluted with `additional test concentrations. dilution Al test water to prepare the four solutions appeared clear and colorless. Exposure vessels: 25 liter polyethylene aquaria containing approximately 20L of solution was 23.2 cm. test solution. The approximate depth of test Number of replicates: two Number of test organisms per replicate: ten Number of concentrations: five pius a negative control Water chemistry during the study: Dissolved oxygen range (0 -- 96 hours): 5.88.5 mg/L (control exposure) pH 5.0-8.6 mg/L (79 range (0 - 96 hours) mglL exposure) 80-84 (control exposure) 7.9-85 (79 mg/L exposure) Test temperature range (0 ~ 96 hours) 21.4-21.8C (control exposure) 21.8-23.7C (79 mg/L exposure) Element Basis: Mortality. Mussels responding to gentle prodding were with open shells considered dead. and not The number of individuals exhibiting clinical signs abnormal behavior also were evaluated. of toxicity or Method of calculating arithmetic mean mean measured concentrations: RESULTS _ Nominal concentrations: <LOQ, 5.7, 11, 23, 46, 91 mg/L EMleeamseunrtevdalcuoen:cen9t6r-ahtoiuornLsC:so<L=O5Q9,m5.g3, 12, 20, (51-68 41, 79 mg/L) mg/L SwtearteisctailccaullEatveadluwahtieonn:poTshsieblLeCSb0y pvraolbuietsanaanldys9is5,%thceonmfoivdienngceavinetreargvaels method or binomial probability with non-linear computer softwareofC.E. Stephan. interpolation using the 0060802 Analytical Methodology: Analyses of test solutions were performed at Wildlife International Ltd. using high performance liquid chromatography with mass spectrometric detection (HPLC/MS). When determining the concentration of the test substance in the test solutions, the same and `most prominent peak response for perfluorooctanesulfonate was used. No attempt was made to quantify on the basis of individual isomeric components. The LOQ (limit of quantitation) was 0.115 mg/L in thisstudy. The mean percent recoveryofmatrix fortifications analyzed concurrently during sample analysis was 94.7%. Samples collected at test initiation had measured values from 73.7% to 96.0% of nominal. Measured values for samples taken at 48-hours ranged from 81.2 to 98.9% of nominal. Measured values for samples taken at 96-hours ranged from 88.5 to 130% of nominal. |mg [mergay []| `Summary of analytical chemistry data: [ec | wetcs | woo| | [or [omumsme|moowm|nwsn| [vw Teminze|son|swoe| [a[sowewr|mm| nooom| [wo |oamammm|somo|snws| Lo [eonnmma|m om|ewns| Biological observations after 96-hours: Mussels in the negative control, the 5.3, 12 and the 20 mg/L treatments appeared healthy and normal throughout the test with no mortality or overt clinical signs of toxicity. Five percent mortality was observed at 96-hours in the 41 mg/L treatment and 90% mortality was observed in the 79 mg/L treatment. No abnormal behavior was noted in these concentrations. ECEET Cumulative percent ee mortality: oe[or| [= | I rE---- os| Ln 0 P) 000503 Control response: Satisfactory cCoOnNcClLuUsSIiOoNnSs 000 The potassium perfluorooctanesulfonate 96-hour LC50 for the Freshwater Mussel, Unio complamatus was determined to be 59 mg/L with a 95% cwoansfi2d0enmcoelli.nterval of 51-68 mg/L. The 96-hour no `mortality concentration Submitter: 3M Corporation, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY Reliability: Klimisch ranking 1. REFERENCES This study was conducted at Wildlife International, Ltd. Easton, MD at the request of the 3M Company. SOTe HER -- Last changed: 5/3/00 000804