Document QJ0ZrBdexVdJyqx6Vrvb2wGb5

ARH2 6 _ 0 6 3 7 ACUTE TO XICITY TO AQUATIC INVERTEBRATES (COROPHIUM VOLUTATOR) TEST SUBSTANCE_______________________________________________ Identity: A mixture containing perfluorooctanesulfonate, which may also be referred to as PFOS, FC-95 or as a component of FC-201AF. (1Octanesulfonic acid) (CAS # 2795-39-3). Remarks: The 3M production lot number was not noted. The test sample is FC-201AF. Current information indicates it is a mixture of 3% PFOS, 18.59% diethylene glycol butyl ether, 37.81% water, 14% ethylene glycol, 8.25% Acrylic foamer, 9% sodium octyl sulfate, 0.3% sodium lauryl sulfate, 3% triethanolamine, 6% sulfonated alkyl ether salts, and 0.05% tolyltriazole. The following summary applies to a mixture with incompletely characterized concentrations o f impurities. Data may not accurately reflect the toxicity o f the fluorochemical component o f the test sample. METHOD:________________________________________________________ Method: ASTM E1367-90, Standard guide for conducting 10 day static sediment toxicity tests with marine and estuarine sediments. Type: Acute static GLP: No Year completed: 2000 Species: Corophium volutator Supplier: Field collected from an unpolluted site near Trondheim, Norway Analytical monitoring: pH and dissolved oxygen Exposure period: 10 days Test organism age: Adults (4-8 mm size) Statistical method: LC50 values calculated by PROBIT Analysis, National Swedish Environmental Protection Board (version 2.3 ,19 90). Calculation of NOEC by single-factor ANOVA followed by Bonferronis procedure. Test conditions: Dilution water: Natural seawater (34 ppt salinity) from an unpolluted site in Byfjorden outside of Stavanger, Norway. Sediment collected at an unpolluted site. The sediment is a muddy sand and had an organic fraction of 0.7% and a silt fraction of 0.8%. Dilution water chemistry: Not given. Lighting: Not given. Stock and test solution preparation: Each test concentration was prepared by adding test substance to 600 g damp sediment and 200 mL of seawater, then shaken for 3 hours. The sediment was split into three subsamples, with an overlay of 400 mL seawater added to each, and aerated overnight before study initiation. Exposure vessels: 600 mL glass beakers containing 200 g of test sediment and 400 mL of seawater, with continuous aeration. 000265 Number of replicates: 3 Number of animals per replicate: 10 Number of concentrations: four plus a blank control Water chemistry during the study: pH range (0-10 days): 8.0 - 8.1 (control exposure) 7.8 - 8.1 (3020 mg/kg exposure) Temperature range: 15 1 C Dissolved oxygen range (0-10 days): 91 - 94 % (control exposure) 49 - 97 % (3020 mg/kg exposure) RESULTS___________________________________________________ Nominal concentrations: Bk control, 92.4, 495,_1030, and 3020 mg/kg (dry weight). Reference substance (fluoranthene) at 20.3 mg/kg dry weight. Element values: 10-day LC50 = >3020 mg/kg (sediment dry weight) 10-day NOEC = 495 mg/kg (sediment dry weight) Element values based on nominal concentrations Remarks: Testing was conducted on a mixture as described in the Test Substance Remarks field. The values reported apply to that mixture and not the fluorochemical proportion alone. C O N C L U S IO N S ___________________________________________________ The test sample 10-day LC50 was determined to be >3020 mg/kg (sediment dry weight). The 10-day No Observed Effect Concentration was 495 mg/kg (sediment dry weight). Validity criteria fulfilled and reference substance was in the normal range. Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY___________________________________________________ Reliability: Klimisch ranking = 2. This study meets the criteria for quality testing. However, sample purity was not properly characterized and the study lacks analytical confirmation of the amount of fluorochemical proportion in the solution. REFERENCES____________________________________________________ Test was conducted by RF Rogaland Research, of Stavanger, Norway at the request of 3M Norge, Lab reference number 99312-1, 2000. 000266 OTHER Last changed: 6/26/00 000267 L99a3b1.r2e-f1.no.: Page 1 of31 (Q l NORSK AKKHEDITERING Nr. POI RROE SGEAALRACNHD "FC-201 AF Light Water Brand AFFF" Toxicity tests Report RF-2000/001 Lab. ref. no.: 99312-1 No. of pages: 31 ISBN: Author: Elin Horve Client(s): 3M Norge Distribution restriction: Version No. / date: Vers. 1/05.01 2000 Research Program: Open from (date): Summary: The following test results were obtained with "FC-201 AF Light Water Brand AFFF': Toxicity: Skeletonema costatum Acartia tonsa Corophium volutator 72 h EC* 48 h LCg, 10 d LCm (mg/l) : (mg/l) : -50 34 (mg/kg dry weight) : >3020 The results relate to the product as supplied by Novatech. Key-words: Toxicity, Skeletonema costatum, Acartia tonsa, Corophium volutator. No part of this document may b e reproduced in any form without permission in writing by RF - Rogaland Research . /5 [ Stud/director / Inger Lisa Andersen - K o , --- for RF - Rogaland Research Kre Netland 000268 L99a3b1.r2e-f1.no.: Page 2 o f31 CONTENTS: 1. Pr e fa c e ..................................................................................................................................................................... 3 2. TEST FOR TOXICITY WITH SKELETONEMA COSTATUM...... ........................................................................4 3. Test fo r toxicity with A cartia tonsa.................................................................................................. 10 4. Test fo r toxicity w ith Co ro ph iu m volutator................................................................................15 5. D a t a sheet(s) ...................................................................................................................................................... 20 000269 L9a9b31.r2e-f1.no.: Page 3 o f 31 PREFACE: Ecotoxicological tests were Novatech. The sample is a performed on TC-201AF concentrate that normally Light will bWeautesreBdriannad AFFF"in agreement 1% solution. Tests with were conducted in accordance with the "Harmonised Offshore Chemical Notification Format" (OSPARCOM1995 / SFT1998). pTehrefoprrmojeedctbwyaRsFc-arMriieljdoloaubt.aSt tRudFy-dRiroegcatolarnwdaRseSseenairocrhEfonrgi3nMeeNr Ionrggeer. LAisllatAhendteesrtssenw.ere The test results arepresented in separate sections. The results relate to theproduct as supplied by Novatech. This test report is issued by a laboratory accredited by Norwegian Accreditation (NA). The accreditation states that the laboratory meets the NA requirements concerning competence, equipment and workingproceduresfo r all the test methods contained in the accreditation. It also states that the laboratory has a satisfactory quality assurance system, which includes icnetretrifnieadl qruefaelriteynccoenmtraotlesrciahlesmaensd, pseacrotincdipaartyiornefeinreinncteermlaabtoerraiatolsr.y comparisons, and the use o f The testsperformed are covered by the accreditation. wThriistintegstbryeRpoFr-t Mmailyjenlaobt .be reproduced in anyform other than infull, without thepermission in 000270 L99a3b1.r2e-f1.no.: Page 4 o f31 Test for T oxicity Marine alga: Skeletonema costatum 000271 L9a9b31.r2e-f1.no.: Page 5 of31 TEST REPORT Grow alga tShkienlheitbointieomn atecstoswtiatthutmh e m a r in e fO l NORSK AKKRNEr.DPOITIERING RR OE SGEAALRACNHD TEST SUBSTANCE: "FC-201 AF Light Water Brand AFFF" TEST RESULT: The 72 hours EC50was calculated to ~50 mg/l. The test substance was supplied by Novatech, and the test results relate to this product. TEST DETAILS: METHOD: NwSit-hESNkeIlSeOton1e0m2a53c,o1s9t9a8tu:mWaantderPqhuaaeloitdya-cMtylaurminetraiclgoamlugtruomw.th inhibition test TEST ORGANISM: Skeletonema costatum, organism is kept at RF -stRraoignaNlaInVdARBesAeaCrc1h.. A stock culture of the test PREPARATIONS: Natural seawater (salinity: 34 %o) from an unpolluted site in Byfjord was used aansdbabsriiseffolyrhtheaetgerdotwothabmouetd7iu5mC. pTrhioerwtoatuersew. aTshfeiltseeraewdattherrowugahs aenGriFc/hCedfiwlteitrh, nutrients according to ISO 10253, and recommendations from the Paris Commission. Test solutions were prepared from a stock solution. A stock solution of 511.2 mg/l was prepared by stirring 0.5112 grams of the product in 1 litre of growth medium. Other concentrations were prepared from this stock solution. INCUBATION: ToTlarehtbseeitdttacetluoslsttahuwbarakoeusesrtro,u2f3n050ru0e0npmdclieelcrlwalscte/eomrsnefltoiifnnrrucoeuomabucashatnlecidgoehnxintcpe(o1n>n0ter50na0mttiipoalEnlfl.ly/antTgr-r-besosoewttktcio)numfglrtueoindrmeofsclfaulwuslukeormrseeoscincnueoalntcnuture. tubes of universal natural white type, at a temperature of 20 2C. The test was run for about 72 hours, and subsamples of 5 ml were taken from bthyeftleusotrfelascseknsceea. chpHdamy efaosrugrreomwetnhtsmweaesrue rpeemrfeonrtms. eAdlgasalingdriocwattehdwoans dmaetaassuhreeedt. 000272 L99a3b1.r2e-f1.no.: Page 6 of31 DATA ANALYSIS: CALCULATIONS: Growth rates were calculated by log linear regression on biomass (fluorescence) versus time, as described in NS-EN ISO 10253. ErCso: Tceofhfneecceitnnchtoribnaitctieoonrntyroaefftifaoenpctrwoodanustchctaeglcgivuriolnawtgethd50ob%yf Sirkneetdeluertpcotoinloaentmiooanfcgboresottwwatteuhemnra3itse2em(Exgpr/Crlesasons).eddTahsisthe lOOmg/1.. NOEC: CfaacltcourlaAtiNonOoVfANOfoEllCow(eNdobOybBsoernvfeerdrEonffisecptrCocoendcuernetrfaotriocno)mipsadroinnge beaycshingle- concentration mean with the control mean. TEST RESULTS: COMMENTS: VALIDATION: Table 1. EC and NOEC values obtained with Skeletonema costatum. Test substance "WFaCt-e2r0B1raAnFdLAiFghFtF" ErCio _ EiC5o EfCo -50 NOEC: 10 CVVraaalluupeehssPianarddeiIccnacStlectauctloa3nt.ec0de. nbtyraitnitoenrspogliavtiinogn.10N%O. E50C%araencda9lc0u%laitnehdibbyitiuosninogftghreocwothmprauttee.rpUronigtrsaimn mmgefl. Td(suhereeinrggersowuwlhttsihcahcruetrhbveaegsse)r.dowotnhgirsocwotnhsirdaetreesdfotrotbheeetximpoensepnatnia0l i-n7t2heh,ctohnetrpoelrcioudltures Tc(ceholelrrdveeasnlpisdoiitntydyiicnnrgcirtteeoraisaaegdgribovywentahifnaractttheoerotoefsfmtmoproreroettohtchaonaln0a,r19e6dfiun'1l)ft,hiltelhetede,csoitnnptterhroailotdpthHe control did not v3a,5r-ydbicyhmloroorpehtehnanol1w,0ausninitsthdeurrainnggethneotremstaallnydfothuend7.2 hours test results with LSaamb.prleef.rneoce.:ived.: Test period: Report date: 2909.30192.9-19 04-07.10.99 05.01.2000 Elin Horve AnalytEicnaglinteecehrnician SenQiAourgaRelieMtsyeoAalvrsceshursrSmacnyiceren_t'ist Enclosed: Data sheets Tfohrims toetshterrepthoarnt iisnisfsuulle,dwbityhaoulatbthoerapteorrmy iascsciorendiitnedwrbiytinNgobrwyeRgFia-nMAiclcjoreladbit.ation (NA). The report may not be reproduced in any 000273 L99a3b1.r2e-f1.no.: Page 7 o f31 PRIMARY DATA: Growth inhibition test with Skeletonema costatum Test substance: "FC-201 AF Light Water Brand AFFF" Lab. ref.: 99312-1 Start date: 04.10.99 Cone, unit: mg/l Measurement: The biomass was measured by fluorescence; values in fluorescence units Time: 04.10.99 05.10.99 06.10.99 07.10.99 Concentration 14:05 09:25 10:30 10:40 Growth PH pH rate Corre- at start at end day-1 lation of test of test 0 0.11 0.59 9.2 56 2.18 0.997 0 0.15 0.63 7.9 59 2.09 0.999 0 0.13 0.62 9 54 2.11 0.998 8.3 8.4 0 0.14 0.63 6.4 47 2.04 1.000 0 0.14 0.62 6.3 48 2.04 1.000 0 0.12 0.71 7.1 55 2.14 1.000 0.1 0.16 0.74 7.2 60 2.07 1.000 0.1 0.13 0.61 5.5 55 2.12 0.999 8.4 8.5 0.1 0.14 0.55 8.3 64 2.14 0.998 0.32 0.32 0.32 1 1 1 3.2 3.2 3.2 10 10 10 0.14 0.14 0.14 0.14 0.15 0.13 0.14 0.16 0.15 0.16 ' 0.15 0.16 0.74 0.68 0.68 0.83 0.88 0.93 0.86 0.76 0.63 0.45 0.46 0.46 8.7 8.6 9.2 8.2 9 8.9 9.2 8.9 7.3 5.5 5.2 6.2 58 63 62 59 61 61 67 73 63 57 52 49 2.11 0.999 2.14 0.999 2.13 0.999 2.11 1.000 2.10 0.999 2.15 0.999 2.16 0.999 2.14 0.999 2.11 0.999 2.06 0.994 2.05 0.996 2.00 0.995 8.4 8.4 8.4 8.4 8.4 8.4 8.5 8.5 32 0.14 0.4 3.4 25.5 1.82 0.996 32 0.15 0.3 2.7 17 1.66 0.991 8.4 8.4 32 0.13 0.4 4.4 33 1.94 0.997 100 0.15 0 0 0 100 0.18 0.01 0.03 0 100 0.13 0 0 0 0.00 0.00 0.00 8.4 8.2 Reference substance (3,5-dichlorophenol): 1.5 mg/l 0.14 0.47 1.85 1.5 mg/l 0.16 0.43 1.45 1.5 mo/l 0.16 0.45 1.45 4.2 3.3 2.9 1.19 0.992 1.06 0.996 1.01 0.992 8.3 8.2 Mean values: 0 0.13 0.6 8 53 0.1 0.14 0.6 7 60 0.32 0.14 0.7 9 61 1 0.14 0.9 9 60 3.2 0.15 0.8 8 68 10 0.16 0.5 6 53 32 0.14 0.4 4 25 4on 4r n r\ r\ n 2.10 2.11 2.13 2.12 2.14 2.04 1r>.8r\0n, 0.999 0.999 0.999 0.999 1.000 0.995 0.995 The result of the reference 0 0 0 2 7 4 L99ab3.1r2e-f1.no.: Page 8 o f31 GROWTH CURVES: Growth inhibition test with Skeletonema costatum Test substance: "FC-201 AF Light Water Brand AFFF" Lab. ref.: 99312-1 .t J test duration (hours) 7 100 m g/l 24 48 72 test duration (hours) L9a9b31.r2e-f1.no.: Page 9 o f31 Inhibition of the growth of Skeletonema costatum with: mg/1 ("FC-201 AF Light Water Brand AFFF") Experimental data: Concentration in Growth rate "FC-201 AF Light Water Brand AFFF" Control 1 Control 2 Control 3 Control 4 Control 5 Control 6 Control mean : 0.10 0.10 0.10 0.32 0.32 0.32 1.00 1.00 1.00 3.20 3.20 3.20 10.00 10.00 10.00 32.00 32.00 32.00 100.00 100.00 100.00 2.18 2.09 2.11 2.04 2.04 2.14 2.10 2.07 2.12 2.14 2.11 2.14 2.13 2.11 2.10 2.15 2.16 2.14 2.11 2.06 2.05 2.00 1.82 1.66 1.94 0.00 0.00 0.00 Inhibition in percent _ * - 0 1.4 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 1.9 2.4 4.8 13 3 p> . 21.0 X/4 7.6 100.0 100.0 100.0 000276 L99a3b1.r2e-f1.no.: Page 10 o f31 T est for T oxicity Marine herbivore: Acartia tonsa 000277 L99a3b1.r2e-f1.no.: Page II o f31 TEST REPORT AMCAURTINEETCOOXPICEIPTOYDTAEScTaWrtITitHt THE tOHSU (8 i NORSK AKKRNEr.OPoITiEl RING RR OE SGEAALRACNHD TEST SUBSTANCE: "FC-201 AF Light Water Brand AFFF" TEST RESULT: The 48 hours LC5owas calculated to 34 mg/1. The test substance was supplied by Novatech, and the test results relate to this product. TEST DETAILS: METHOD: ItoSOm/aDrIinSe1c4o6p6e9p-od1s99(C7:oWpeaptoedraq,uCarliutsyta-cDeae)t.ermination of acute lethal toxicity TEST ORGANISM: Acartia tonsa (Dana), supplied by the Marine Biological Laboratory, University of Copenhagen. Cultivation: The test organisms were supplied as eggs, and were cultivated in accordance with the "Paris Commission - Ring Test Protocol" (VKI). Large copepodites and adults were used in the test. PREPARATIONS: Nasatbuarsaisl prior to usfeosaerw.thaetedri(lusatiloinnitwy:at3e4r.%To)hferowmataernwunaps ofillltuetreeddstihteroinugBhyafjGorFd/Cwafisltuesred Tmdieglsu/t1tiswoonaluswtpiaortneeprs.awrOeedrthebepyrrcsetopinrarrcieendngtfr0rao.4tmi9o9na7sswtgorecarkme ssporoleufpttaihorened.prfAordosmutcottchkiniss0os.tl5uoctliikotrnseooolufft9io9n9..4 INCUBATION: The test was run in glass containers (28 ml rolled rim vials) each containing 25 ml of test solution and 5 animals, 4 replicates for each concentration. The vials were kept under a 16h/8h light/dark photoperiod at a temperature of 2d0ata s1heCet.. pH and oxygen measurements were performed as indicated on the 000278 J n o i I -----------------,, KWwm/ Tl+4751 8750 00.Fax:+4751 875200.Entorprijenumber.:950884800. L99a3b1.r2e-f1.no.: Page 12 o f 31 DATA ANALYSIS: CALCULATIONS: The effect on the survival of Acartia tonsa is expressed as the concentration of paeprrtoedsutcctolnectehnatlrtaoti5o0n%weorfethuesetdesftoarnciamlcaulsla(tLiCon50. )T. hTeh2e4cohmoubrisnaenddm4o8rthaoluitrys LstCa5n0davradlupersoacenddutrheeP9R5%OBcIoTnAfidneanlycseisli,mNiattwioenraelcSawlceudlaisthedEbnyviurosinnmgetnhteal Protection Board (Version 2.3,1990). This programme also calculate test concentrations lethal to 10% and 90% of the test animals. NOEC: Calculation of NOEC (No Observed Effect Concentration) is done by single factor ANOVA followed by Bonferronis procedure for comparing each concentration mean with the control mean. Test RESULTS: Table 1. LC and NOEC values obtained with:"FC-201 AF Light Water Brand AFFF" Acartia tonsa 24 hours 48 hours LC10 20 (10-29) 12 ( - 29) . LC5o 52 (37-71) 34 ( - ) LC90 130 (92-260) 93 (39- ) NOEC: 32 10 ctVhaaellcucueolsmaitpneuddtibecyraptPerRo1Og0r%Ba,ImT5m0aen%aGlayrnsaidps,9h0aP%naddl9Ien5thS%atalcittoy3n.a0fif.dteern2ce4ianntedr4va8lshoaurres.indUincaittsedin. mNgO/lE. CVaarlueecsaalcreulated by VALIDATION: The validity criteria given in the test protocol are fulfilled, in that the dissolved oxygen concentration at the end of the test was greater than or equal to 4 mg/l, the percentage mortality in the controls was less than 10%, and the 48 hours LC50 of 3,5-dichlorophenol (reference) was in the range normally found. Lab.ref.no.: TSRaeemsptopprleterdriaoetdce:e:ived.: 99312-1 0065-20.0081..01.2900..990990 EElinngHinoerevre Age Molversmyr Senior Research Scientist Analytical technician Quality Assurance Enclosed: Datasheets Tfohrims toetshterrepthoarnt iisnisfsuulle,dwbityhaoultabthoerapteorrmy iascsciorendiintewd rbiytinNgobrwyeRgFia-nMAiclcjareladbit.ation (NA). The report may not be reproduced in any 000279 L9a93b1.r2e-f1.no.: Page 13 o f 31 PRIMARY DATA: Acute toxicity test with Acariia tonsa Test substance: "FC-201 AF Light Water Brand AFFF" Lab. ref.: Start date: Measurement: 99312-1 06.10.99 Number of dead Concentration (mg/l) Control 10 32 100 320 999 Total number Number of specimen pr. vessel Specimen A C E pr. cone. B D F 5 55 30 5 5 5 5 55 20 5 5 55 20 5 555 20 5 5 55 20 5 5 55 20 5 ' NUMBER OF DEAD 24 hours 48 hours ACE BDF Total ACE BDF Total 0 00 0 01 -o o o 1 101 2 001 0 1 11 10 3 0 20 1 4 44 5 121 31 -5 5 55 17 5 20 555 5 5 55 20 5 20 5 55 5 55 5 20 5 20 - Reference substance (3,5-dichlorophenol): 1.0 mg/l 5 55 020 2 54 30 5 55 100 3 130 The result with the reference substance is within the range normally found. 15 000280 L99a3b1.r2e-f1.no.: Page 14 o f 31 Test substance: "FC-201 AF Light Water Brand AFFF" Lab. ref.: Start date: 99312-1 06.10.1999 Measurements of oxygen and pH. Start 24 hours 4E hours Concentration (mg/1) Oxygen (mg/1) pH Oxygen pH Oxygen (mg/1) (mg/1) Control 7.6 8.1 7.2 10 7.6 8.1 7.0 32 8.1 8.1 6.5 100 7.7 8.1 5.6 320 7.7 8.1 6.6 8.1 999 7.5 8.1 6.5 8.1 pH 8.1 8.1 8.1 8.0 Reference substance (3,5-dichloropheriol): 1.0 mg/l 7.7 8.1 7.3 8.2 000281 L99a3b1.r2e-f1.no.: Page 15 of 31 T est fo r toxicity Marine Amphipode: Corophium volutator 000282 L9a9b31.r2e-f1.no.: Page 16 oj 31 TEST REPORT ACUTE TOXICITY m arine am phipo SdEDCIMorEoNpThTEiuSTmWvIToHluTHtaEtor (S ) NORSK AKKREDITERING Nr. P01I RROE SGEAALRACNHD TEST SUBSTANCE: "FC-201 AF Light Water Brand AFFF" TEST RESULT: The lOd LC50 was calculated to >3020 mg kg'1dry weight sediment. The test substance was supplied by Novatech, and the test results relate to this product. TEST DETAILS: METHOD: ATSoxTiMcityET13es6t7s-9w0i,thStManadrainred aGnudidEestFuoarriCneonSdeudcimtinegnt1s.0 day Static Sediment TEST ORGANISM: Corophium volutator, from an unpolluted site near Trondheim. CULTIVATION: The test organisms were supplied as adults (4-8 mm in size) and were held in aerated seawater at 15C. A small amount of natural seaweed was used for feeding. Pr e p a r a t io n : NStaatvuarnalgesreawwaasteurse(sdalfionritdyi:lu3t4io%now) afrteorm. aSneduinmpeonlltucteodllescitteedinaBt aynfjourndpeonlluoutetdsidsiete was used for preparing test sediments. The sediment is a muddy sand and was analysed for organic content and proportion silt for each test. Each test concentration was prepared by adding the product in 600 grammes boufcdkaemtpwsaesdsihmaeknetninfoar p3lahsotuicrsb.uTckheet.se2d0im0 emnlt owfassedawivaidteerdwinatsoatdhdreeed baenadktehres, 400 ml of seawater was added to each, and the beakers were aerated over night. 10 animals were then randomly selected for each beaker. A sample of tchaelcdualamtepdsaeds immgenktgw'1assedainmaelynsteddrfyorwweiagthetr. content, and all concentrations are INCUBATION: The test was run in glass containers (600 ml) each containing 200 g of test sceodnicmenetnrta,taiopnp.. 4T0h0embelaokfesresawwearteerkaenpdt u1n0daenricmonaltsin, 3uoruesplaicearatetisofnorateaach tinemdipceartaetduorenothfe15data1shCe.etp.H and oxygen measurements were performed as 000283 _^_G__R_ Professor Olov Honssensvei 15, P.O.TBSo.-x--2--50-3--U--l-la.ndhausg&,w40F9W1 nSntnav. aNnogrewr,aNy.oTrewl.a:y+.4T7el5.:5+4574 5318 8570.5F0a0x0: +. 4F7ax5:5+45745318 867052 00. Enterprise number.: 950884800. L9a9b3.1r2e-f1.no.: Page 17 o f31 DATA ANALYSIS: CALCULATIONS: The effect on the survival of Corophium volutator is expressed as the concen tration of a product lethal to 50% of the test animals (LC50). The combined mortality per test concentration were used for calculation. The value and the 95% confidence limit are calculated by using the s1t0anddaayrdLC50 P2.R3O,1B9I9T0)A. nTahlyissips,rNogartaiomnmaleSawlseodcisahlcEulnavtierotensmt ceonntaclenPtrroatteicotniosnleBthoaalrtdo(V10e%rsiaonnd 90% of the test animals. NOEC: Calculation ofNOEC (No Observed Effect Concentration) is done by single factor ANOVA followed by Bonferronis procedure for comparing each concentration mean with the control mean. TEST RESULTS: Table 1. LC and NOEC values obtained with: "FC-201 AF Light Water Brand AFFF' Corophium volutator 10 days LCio 800 LC50 >3020 LC90 >3020 NOEC: 495 GVVaarllauupeehssPianarddeiIccnaaStlectau1tl0a3%t.e0.d. 5b0y%PRanOdB9I0T%anlaeltyhsailsi.tyNaOfteErC1a0rdeacyas.lcuUlanittesdibnymthgekcgo'1msepduitmerepnrtodgrryawmemigeht. VALIDATION: TpFelhureocevrnaantliatdgeinetyem(correritfteearrliieatnygciienv)etwhneaisncoitnnhtetrhoteelssrtawpnergroeetnoloecosrsml atahrlealynfuf1ol5fui%lnledad,n,bdiuntattthheaesttdtwhiseistohlved cooxnycgeenntrcaotniocnenst(r3a0t2io0nmwgasklge's1sdtrhyawneriegchotmsmedeinmdeendt)(6a0fte%r )fiivneodnaeyos fatnhdeitnesotne aocfctehpetreedplliimcaittessfoarfteorrgteannidcacyosn. teTnhtebsuetdtihmeesnilttufsreadctiiosnfoiusnrdectoombme ewnidtheidnhigher. Table 2. Characteristics ofthe sediment used for testing Organic content (%) 0.7 Silt fraction(%) 0.8 LTSaaebmst.pprleeefr.rionedco:e.:ived.: Report date: 99312-1 0035-12.0301..01.2920..990990 AnalyEtEliicnnaglHintoeercevhrenician SenQiAourgaRelieMtsyeoAalvrsceshrusrSmacnyicerentist Enclosed: Datasheets Tfohrims toetshterrepthoarnt iisnisfsuulle,dwbiythaoulatbthoeraptoerrymaiscsciorendiintedwbriytinNgobrwyeRgFia-nMAiclcjoreladbit.ation (NA). The report may not be reproduced in any 000284 L99a3b1.r2e-f1.no.: Page IS of 31 PRIMARY DATA: Acute toxicity test with Corophium volutator Test substance: "FC-201 AF Light Water Brand AFFF" Lab. ref.: Start date: Measurement: 99312-1 03.12.99 Number of dead Total number Number of specimen pr. vessel Concentration Specimen A C E (mg/kg dry weight) pr. cone. B D F Control 10 10 10 60 10 10 10 92.4 10 10 10 30 10 10 10 495 30 1030 10 10 10 30 3020 10 10 10 30 NUMBER OF DEAD 5 days 10 days ACE BDF Total ACE BDF Total 0 00 -- 000 0 100 1 0 11 2 12 1 4 122 5 - Reference substance (fluoranthene): 20.3 10 10 10 30 3 22 The result with the reference substance is within the range normally found. 7 000285 L99a3b1.r2e-f1.no.: Page 19 of31 Test substance: "FC-201 AF Light Water Brand AFFF" Lab. ref.: Start date: 99312-1 03.12.99 Measurements of oxygen and pH. Start Concentration Oxygen (mg/kg dry weight) (%) pH 5 days Oxygen (%) pH Control 94 8.1 91 8.1 92.4 94 8.1 76 8.1 495 94 8.1 91 8.1 1030 95 8.1 87 8.0 3020 97 8.1 49 7.8 10 days Oxygen (%) pH 91 8.0 88 8.1 89 8.1 89 8.1 77 8.0 Reference substance (fluoranthene): 20.3 95 8.1 90 8.1 90 8.1 000286 L99a3b1.r2e-f1.no.: Page 20 o f 31 D ata Sheets 000287 L9a9b3.1r2e-f1.no.: rU'gK A yj -fJ HMS-DATA3LAD (Heise-, Miljo- og Sikkerhetsdatablad) Dokumentr.: Erstattet : 10-4145-6 Utgitt : 11/11/98 Versjon - : 06.01 03/11/93 Skrevet ut : 04/01/99 Side 1 av 11 KMS-dacablacet er utarbeidet av Miljaavdelingen ved 3M Norge A/S. Telefon: S3 84 73 00 / Telefax: 63 84 17 88 1 IDENTIFIERSJON AV KJSMIKALIET OG ANSVARLIG FIRMA. Importer: 3M Norge A/S Har.de1.3navr. : FC-602 LIGHT WATER BRAND ATC PLUS Deklaras jonsnummer, Produktregisteret: ?-nr.: 035337. Telefon, Giftinformasjonssentralen: 22 59 13 00 j I 2 OPPLYSNINGER OM KJEMI5X SAMMENSETNING. Informasjon on enkeltkomponenter, faresymbol(er) og risikosetninger STOFFNAVN CAS-nr VEKT-PROSENT VANN 1,2-STANDIOL Faresymboler/risikosetninger: Xn; 2-{2-BUTOKSYSTQKSY)-ETANOL / (DIETYLENGLYX0LM0N03UTYLETER) Faresymboler/risikosetninger: Xi; FLUORALKYL OVERFLATEAKTIVE STOFFER SYNTETISKE OVERFLATEAKTIVE STOFFER MAGNESIUMSULFAT TYXNERE RESTER AV ORGANISEE FLUORKJEMIKALIER 7732-18-5 107-21-1 22 112-34-5 36 Trade Secret Trade Secret 7487-88-9 Trade Secret Trade Secret 58 - 63 = 20 = 12 1-5 1-5 =1 0.5 - 1.5 Ikke kjent i 00288 L9a9b31.r2e-f1.no.: Page 22 o f 31 FC-602 LIGHT WATER BRAND ATC PLUS 3 VIXTIGSTE FAREMOMENTZR. Vikzigsaa skadevirkninger Helsefare : Kan irritare oynene, huden og luftveier.e. Sikkerhet: Miljofare: Spesialavfall. 4 F O R S T :j z l ? s TAX. Tiitak evr.e: Skyll straks oyner.e med rikelige menge er vana. Kontakt lege umiddelbart. Tiitak had: Skyll huden med rikelige mengcer vann. Hvis irritasjonen vedvarer, konaakt lege. Tiitak inninding: Kvis symptomer oppstr, bring vedkommer.de bort fra eksponeringskilden..Sorg for frisk luft. Hvis symptomene vedvarer, kontakt lege. Tiitak svelging: Kontakt lege umiddelbart. Fremkail brekninger kun i samrad med medisinsk personell. Gi aldri noe gjennom muaaen til en bevisstlos person. 5 TILTAK VED BRANNSLUKNING. Egnet brannslukningsmiddel: Produktet er et brannslukkingsmiddel. Ikke egnet brannslukningsmiddel: Eksponeringsrisiko ved brann: L9a9b31.r2e-f1.no.: rage 3 oj 31 FC-602 LIGHT WATER BRAND ATC PLUS Forbrennir.gsprodukter ved brann: Prosedvrer ved slukking av brann: ii 6 TILTAK VED UTILSIKTET UTSLI??. Personlige vernetiltak: Se vernetiltak under andre punkter i dette EMS-databLadet. Tiitak ved utiisiktet utslipp: Ventiler omracet. Spill avgrenses umiddelbar^. Dekk til med et absorberende materiale. Spill samles opp umiddelbart. Rengjor omridet med var.n. Plasseres i en egnet beholder med lokk. Fjerning av kjemikaiieavfall: Se punkt 13, Fjerning av kjemikaiieavfall. i iI 7 HNDTERING 0C- 0PP3EVARING. j ___________ *______________________________________________ .______________ I Betingelser for sikkar oppbevaring: M ikke lagres i omrder hvor produktet kan komme i kontakt med mat eller legemidier. Lagres ved temperatur uncer 49 grader celsius. Lagres ved temperaturer over 0 grader celsius. Hold beholceren tett lukket nr produktet ikke er i bruk. j- Uforenelige materialer: Ventilasjon: Oppbevar emballasjen pk et godt ventilert sted. Sikkerhetstiltak for forhindre brann: Sikkerhetstiltak for forhindre eksplosjon: Ikkebrennbar. Sikkerhetstiltak for forhindre statisk elektrisitet: Forholdsregler ved bruk: Hold emballasjer. tett lukket. Rayking forbudt: Under bruk av produktet kan royking forrsake forurensning av tobakken og/eller rayken og farlige dekomponeringsprodukter kan dannes. Se punkt 10, stabilitet og reaktivitet, skadelige dekomponenter. 00290 L99a3b1.r2e-f1.no.: Page 24 o f31 FC-602 LIGHT WATER BRAND ATC PLUS 3 e x s ?o n e r :i:g s x o n t r o l l c g p e r s o n l i g v e r n s u t s t y r Qyevern: Unnga a fa damp, spray eller take i oynene. Bruk tette vernebriller med ventiler. Kindverr.: 3ruk egaede vernehansker av hensiktsmessig materiale ved hindtering av produktet. Veraehaasker av folgende materiale(r) anbefales: Butvlgummi. Aanet hudvern enn hindvern: Unr.gi hudkontakt. Andedrettsvern: Unngi ir.ninding av luftbiret materiale. Basert pit koaseatrasjonen av luftforurensainger i arbeidsatmosfarea velges er. av folgende type(r) godkjer.t incedrettsvern: halvmaske med filter mot orgar.iske damper og vasskeformige partikler: A/P2SL. Svelgiag: Det mi ikke spisas, drikkes eller roykes under arbeid med produktet. Vask utsatte omrider grundig med sipe og vann. Vask hendene etter hindtering og for spising. Anbefalt vaatilasjon: Kvis utsugningsventilasjon ikke er tilstrekkelig, mi egnet godkjent indedrettsvern benyttes. Sorg for tilstrekkelig veatilasjon slik at dampkonsentrasjonene holdes under anbefalte administrative normer og/eller kontroller spray eller tike. Adm. normer for forurensning i arbeidsatmosfare, Februar 1996: VANN (7732-18-5) Aamerkaiager: lagen adm. norm. 1,2-ETANblOL (107-21-1) 8-timers verdi ppm: - (stov) 8-timers verdi mg/m3: 10 (stov) Takverdi ppm: 25 (damp) Takverdi mg/m3: - (damp) 2-(2-BUTOKSYETOKSY)-ETANOL / (DIETYLENGLYKOLMONOBUTYLSTER) (112-34-5) 8-timers verdi ppm: 35 Anmerkainger: Amerikansk grenseverdi anbefalt av produsenten. (Ingen norsk adm. norm). FLUORALKYL OVERFLATEAXTIVE STOFFER (Trade Secret) Anmerkainger: Ingen adm. norm. SYNTETISXS OVERFLATEAXTIVE STOFFER (Trade Secret) Anmerkainger: Ingen adm. norm. 000291 L9a93b.ir2e-f1.no.: Page 25 of31 FC-602 LIGHT WATER BRAND ATC PLUS -- (jvj r-j ) Anmerkninger: Ingen adm. norm. TYXNERI (Trade Secret) Anmerkninger: Ingen adm. norm. b RESTER AV ORGANISXZ FLUORKJEMIXALIER {'Trade Secret) 8-rimers verdi mg/m3 : 0,1 Grenseverdi anbefalt av 3M. 9 FYSI5XE OG KJEMISXZ EGENSKAPSR. Tiistandsform, farge, lukt: Ugjennomsiktig, ravfarvet, viskos vaske. pH: ca. 8.5 Kokepunkt/-intervall: ca. 93.00 C (3egynnende) Smeltepunkt/-intervall: Ikke relevant Flammepunkt: Selvantennelighet: Eksplosjonsgrenser, nedre: Ingen (Setaflash CC) Ikke relevant Ikke relevant Eksplosjonsgrenser, ovre: Ikke relevant Damptrykk: ca. 16 mmHg (Kalkulert v/20 C) Laselighet i vann: 3landbar. Spesifikk vekt: ca. 1.05 (vann=l) Darapcetthet: ca. 0.85 (luft=l) (Xalkulert v/20 C) Flyktige organiske forbindelser (VOC): 339 g/1 SCAQMD (Kalkulert v/20 C) Fordampningshastighet: < 1 (butylacetat-1) Viskositet: Ikke kjent ! | 10 STA3ILITET OG REAXTIVITET. ( L9a93b1.r2e-f1.no.: Page 26 of 31 FC-502 LIGHT WATER BRAND ATC PLUS Forhoid som skai unngis : Materiaier som skai unngis: Ikke kjer.t. - Farlige spaitningsprodukter: Karbonnonoksid og karbor.dioksid. Hydrogenfluorid. Giftige damper, gasser eiler partikier. Termisk dekomponering av bruksferdig blandir.g forventes ikke representere noer. risiko. Stabilitei og reakrivitet: Stabili. Farlig polymerisasjon vii ikke forekomme. 11 OPPLYSJTiGsa OM EELSEFARE. Effekter ayne: Mild ayeirritasjon: symptomer kan vare redhet, hevelse, smerter og tirer Effekter huc: Mild hucirritasjon (etter langvarig eller gjentatt kontakt): symptomer kar. vare radhet, hevelse og klae. Effekter innanding: Er. enkelt overeksponering som overstiger ar.befaite administrative normer, kan fare til: Pvirkning av sentralnervesystemet: symptomer kan vare hodepine, arhet, savnighet, manglende koordinasjonsevne, nedsatt reaksjonsevne, slaret tale, svimmelhet og i ekstreme tilfeller bevisstlashet. Irritasjon (avre luftveier): symptomer kan vare srhet i nese og svelg, hosting og nysing. Effekter svelging: Dyreforsak utfart med organiske fluorkjemikalier, som finnes i dette produktet, viser effekter som leverforstyrrelser, vekttap, manglende appetitt, slavhet samt effekter som pvirker neurologi, bukspytt, binyre og hematologi. Det foreligger ingen kjente helseeffekter for mennesker fra eksponering overfor organiske fluorkjemikalier nr de brukes som forutsatt og i henhold til veiledning. Svelging kan fere til: Pivirkning av nyrene: symptomer kan vare redusert urinmengde, blod i urinen og ryggsmerter. Aspirasjonspneumoni (Lungebetennelse som skyldes at fremmedlegemer 930293kommer ned i lungene. En alminnelig irsak til tilstanden er L9M93o1.r2e-j1.no.: rage z / oj 31 FC-602 LIGHT WATER BRAND ATC PLUS oppkast.): symptomer kan vare hosting, pustevansker, gisping, hoste opp blod og lungebetennelse som kan vare livstruende. Informasjon om allergi, overfalsomhet: " Kreftf remkalier.de egenskaper : Arvestoffskadelige egenskaper: Reproduksjonsskadelige egenskaper: Andre skadevirkninger, helse: Dette produktet inneholder en elier fiere organiske fluorkjemikalier, som muligens kan absorberes og lagres i kroppen over ler.gre tic, er.ten som utgangsmolekylet elier som r.edbrytningsstof fer (metabo litter), og kan akkumuleres ved gjer.tatte ekspor.eringer. Det foreiigger ingen kjente helseeffekter for mennesker fra eksponering overfor organiske fluorkjemikalier r.r de brukes som forutsatt og i henhold til veiledning. Forekomster. av organiske fluorkjemikalier i blodet til den generelle befolkningen og underpopuasjoner som arbeidere, har vart p.ublisert siden 1970 arene. 3Ms epidemiologiske studier av egne arbeidere viser ingen skadelige effekter. Et resym' vedrarende produktets giftighet er tilgjengelig p foresparsei. 12 OPPLYSNINGSR OM MILJ0FARE. Persistens/3iologisk nedbrytbarhet : Dette produktet inneholder en elier flere organiske fluorkjemikalier som muligens ikke er nedbrytbare og forblir i miljeet (persistente). akotoksikologiske data: Et 3M milja-produktdatablad er tilgjengelig p foresparsei. Andre skadevirkninger, milja: Nedbrytbarhet: COD (Chemical Oxygen Demand): 0,552 g/g; BOD 5 dagers (Biochemical Oxygen Demand): 0,254 g/g; BOD 20 dagers: 0,422 g/g; 20 dagers BOD/COD: 0,76. Akvatisk giftighet: ______ 96 timers LC50 for fisk (Fathead minnow (Pimeohales promelas)): > L9a9b3.1r2e-f1.no.: Page 28 o f 31 FC-602 LIGHT WATER BRAND ATC PLUS 1000 mg/1; 48 timers EC50 for daphnia (Daphnia magna): 1000 mg/1; 5 roinutzers og 15 minutters EC50, Photobacterium phosphoreum (Microzox System): henholdsvis 210 mg/1 og 220 mg/1. * Sorg for avfallshr.dtering ihz. gjeldende forskrifter. 13 FJERNING AV XJEMIXALIEAVFALL. Metoder for fjerning av kjemikalieavfall : Spesialavfall. Lever avfall til en offentlig godkjent innsamler, rncttaks- eller behar.dlingsanlegg for spesialavfall. Ar.befalte avfallsgrupper : KOVSDXATEGORI 07: AVFALL FRA ORGANISEE PROSSSSER. NDERXATSGORI 07 06: Avfall fra P3DB av fettstoffer, sipe, rengjoringsmidler, desinieksjonsmidler og kosmetikk. EAX-koce 07 06 03: Halogenerte organiske lasemidler, vaskevaskef og mocerlut. Avfallstoffnummer: 7151 Organisk avfall med halogen. Spesielle kompor.enter: Etyler.glykol, fluorforbindelser. 14 OPPLYSNINGER OM TRANSPORT. ADR-klasse: sifter: bokstav: margr.ummer : CEFIC-kort: UN-nummer: ICAO-klasse: IMDG-klasse: IMDG-side: EKS-nr.: MF AG: 000295 L99a3b1.r2e-f1.no.: Page 29 of31 FC-602 LIGHT WATER BRAND ATC PLUS reviderze merkeforskrifter av januar 1993. Symbol: Ingen Ir.neholder: 1,2-etandi.ol, 2-( 2-butoksyetoksy)-etanol Kan irritere aynene, huden og luftveiene. Mi bare anvendes p godt ventilerte steder. Unng inninding av damp/spraytetike. Unngi kontakt med aynene og langvarig kontakt med huden. Fir man stoffet i aynene; skyll straks grundig med store mengder vann og kontakt lege. Etter hudkontakt, vask med sipe og vann. Mi ikke svelges. Ved svelging, drikk to glass vann og kontakt lege umiddelbart. Oppbevares utilgjengelig for barn. YL-gruppe: 00 L9a9bi l.r2e-f1.no.: Page 30 of 31 FC-602 LIGHT WATER BRAND ATC PLUS Spesielie lover og bestemmelser: 1. Forskrifc om ucarbeidelse og distribusjor. av Helse-, Miljo- og Sikkerhecsdacablad for farlige kjemikalier. 2. Forskrifc on klassifisering og merking av farlige k jestikalier. 3. Forskrifc ora klassifisering, merking m.v. av stoff son kan medfare miljafare. 4. Forskrifc ora scoffkartotek. 5. Forskrifc ora spesialavfail. 6. Ecc. 16 ANDRE OPPLYSNINGER AV 3ETYDNING FOR BRUKERNES SIKKEREET OG HELSE. Anvendesesomrder: Filmdannende skumkonsentrat for slukking av brann i hydrokarboner og polare lesemidier eller vasker. Vikcigsce kilde(r) ved ucarbeideiser. av HMS-databladet: Databiad fra 3M General Offices, St. Paul, Minnesota, USA. II I Ii II I !I i II I rsak til oppdatering: Hele dacabladet er revidert. Tilpasset nye merke- og HMS-databladforskrifter, fastsatt i januar 1998. Etc. Egr.e opplysninger: Inforraas jonen p dette .'KMS-databladet er i henhoid til vare nlvarende opplysninger og besce oppfatning angende riktig bruk og hndteringen av dette produktet under normale forhold. Enhver bruk av produktet som ikke er i samsvar med dette HMS-databladet, eller som medfarer bruk av produktet i kombinasjon med andre produkter eller enhver annen prosess er brukerens ansvar. ; j i j j. | | ! 000296 L9a9b3.1r2e-f1.no.: Page 31 o f31 FC-602 LIGHT WATER BRAND ATC PLUS F0LGENDE PROOUXTER DEXXES AV DETTE HMS-DATA3LADET : 3M ID XF-0008-0221-5 3M AFFF/ATC FC-602 Brannsluknin gsskum Produksjon 3H ID-r.r. : XF-0008-0100-1 3M Light Water3/6%AFFF/ATC Fire Fighting Foam, FC-602,1000 L 3M ID-r.r. 3'A ID-r.r. : XF-0008-0078-9 3 Light Water3/S%AFFF/ATC Fire Fighting Foam, FC-602, 20 L : XF-0008-0077-1 3M Light Water3/6%AFFF/ATC Fire Fighting Foam, FC-602, 200 L I i I I i t I j ! i ! !I i t OOl>297