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AR 226 . 0700 ACTIVATED SLUDGE RESPIRATION INHIBITION TEST EST SUBSTANCE Identity: ArefmeirxrteudretocaonstPaiFnOiSng, pFeCr-fi9u5orooroacstaanecsoulmfpoonnaeten,twohfiFcCh-m2a01yAaFl.so(b1-e Octanesulfonic acid) (CAS# 2795-39-3). iRseFmCa-r2k0s1:AF.TChuerr3eMntpirnofdourcmtaitoinonloitnnduicmabteesrwitaiss anmoitxntoutreed.ofT3h%e PteFsOtSs,ample 81.82.55%0%Acdriyeltihcyfloeanemegrl,yc9ol%bsutoydlieutmheorc,ty3l7s.u8l1fa%twea, t0e.r3,%1s4o%dieutmhyllaeurnyel gsluylcfoalt,e, 3% triethanolamine, 6% sulfonated alkyl ether salts, and 0.05% tolyltriazole. cThhaerafcotlelroiwziendg scounmcmeantrryataipoplnisoesf tiompaurmiitxiteusr.e Dwaittah imnacyomnpoltetaeclcyurately reflect the toxicityofthe fluorochemical componentofthe test sample. METHOD TMeestthotdyp:e:OEStCatDicGauciudetleine 209 YGeLaPr:CNomopleted: 1995 ASntaatliysttiiccaall mmoentihtoodrsi:ng:ResDuilstssolwveerdeodxeytgeernm,itneemdpebryatcaulrceu,laatnidonpoHf.the % inhibition in respiration rate. TMeetsrtooWragsatneiwsamtseoruTrrceea:tmAecnttivPaltaendt,sSltu.dPgaeulm,ixMeNd liquor collected from the Test condition: SDiylnutthieotniwcasteerw:agMei:llippeorreOMEiClDl-mQTMetwhaotder rReeffeerreenncceesuabnsdttaenscet,s3o,l5u-tdiiocnhiporreoppahreanotli,onw:asApsrteopcakrseodlubtyiodnisosfotlhveing w5a0t0erm,gthienn1b0mrLoTuNtgNo ahthOteH,podiinltuotfeditnoci3p0iemnLt pwrietchipMiitlaltiipoonrewiMtihl1iN-QHTMzSO4 wanads dmielautseudrteod1tLowbieth7.M3i.llT-eQsTMt swuatbesrt.anTcheewpaHsoafdtdheedrdeifreecrtelnycteostohleution TtNeeusstmtvbeveserssesolesfl.sc:oncNeonttgriavteino.ns: one plus blank control, in duplicate and 1 Treefmepreernacteusrueb:sta1n9c.e3-20.7C. fTionatla:l1s.u7sgp/Le)n, dineidtiasloplHid6s.0anadndpfHinoalnpdHay6.5o.f testing: 4.2 g/L (TSS in cEolnesmuemnpttiBoan.sis: Respiration inhibition as determined by oxygen 000308 Remarks: Uptake rates corrected to 20C for calculations. RESULTS Nominal concentrations: Blank controls and 1000 mg/L in duplicate plus one reference substance at 10 mg/L. Element values: 30-minute EC50 = >1000 mg/L 3-hour EC50 = >1000 mg/L Element values based on nominal concentrations. Remarks: Substance Testing was conducted on Remarks field. The values a mixture reported as described in the Test apply to that mixture and not the fluorochemical proportion alone. CONCLUSIONS The test substance 3-hour ECs; for activated sludge respiration inhibition was determined to be >1000 mg/L. Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY - tReesltiiabnigl.itHy:oweKvieirmi,sschamrpalnekipnugri=ty2.waTshinsotstpurdoypemreleytcshatrhaectcreirtiezrieadfaorndqutalhietsytudy lacks analytical confirmation of the amountoffluorochemical proportion in the solution. REFERENCES TSth.isPasutlu,dyMNw,asLacbonRdeuqcuteesdtbnyutmhbee3rMN2C9o6m4p,an1y99,5.Environmental Laboratory, OTHER eer ------ Last changed: 6/26/00 000309 : BEST COPY. AVAILABLE LA n A296: 4 ENVIRONMENTAL LABORATORY Analyst CALS EBis L ACTIVATED SLUDGEMIXEiyDLUOR Page [of 7 TypeTest OECD Ailinted. Spe fospies iio L[how Tec] #209 CIATED SUEWXEPLIQUOR % a ELE er le BEC eViDxatceaS ObLtiaaintedSus_p9e/n7d4ed7/S9o5lo~s ate (MLSS)_1.2 Used 7/274/35 3 Ipuedo Son Bio idssien finnal st sotwton__ LT on. SSEpRero gies 0 SEs dai : BU ial pi G0 (1st test soi. prepared) 1hpap C'S' (astsoln. prepared) PRs Commons "4 e Lobing sludee sole al Ll cud dediuted vol FILL be lager Pee Sn BE TTR Li Jofeostsnistance i G: #3 jon {Description FC = 2C) Afi i S ct e ke Ra a ci Era ra | SR cn RG Ea ES H D LN TTR ERT nen ald DRA ed JR]E : RR eEeGsCatnin A 0 RL e r e panel iJRE OsUr=CanomionsY Afe E; : Hip LA } Be iment 1 SR | Teme So GU]Se 4 ] Ee i eto RARE a 7g 000310 a pa 267 |- BEST COPY AVAILABLE ; ENVIRONMENTAL LABORATOR,Y r_N29GY. " (OHECTD ACTIVATETDesStLsUDoGuEmRoEnSPsIRPARTEIPOANRIANTHIIOBNITIONTEST#209 semenpoSlOeEPOB AE E: o E. Sample Description: Aub: {ug Te = 3s E E Aratysr _GIT 4 ; ; 3i E ou: aff a fo 5 tesco mpaided i mibolwai mol parS00 mli#perS00ml mio omage dma 70 ToalVoume fpeederS0a0l inocutum per 500mL EI ml i 00 HTS C Z0 0 BET oe TERT ps i cif. BEET pe Er ER TI zt oT esusesaas Se AMRAEI J Bless RRR a ADOBUR NFI i TAS 2%) s R ATS N N sveE TOMS B | : iisfson AAR RISN ES MS SSUAR04A ESAP SETERR S E SRS A S rA ti s A 7RI S D ORAS 5 BPA RAA DNR R 30% Ratd eR : EE ------ ] Se i Reo et | POE ER RETR = 000311 PM 3eF7 BEST COPY AVAILABLE La N2ICY. " ECD ACTIVATEENDVSILRUODNGMEENRTEASLPIRLAATBIOONRANTHOBRIYION TEST 1200 Bid RAW DATA ; E Test substance:CEG OIAE ei R b Source of Aviva, Sindon Moca vior:fe os [este [ih Fuca BltSIR)ron) "outa Recued: 2)iijas outa sec: 4 boa GD BIG aE TVi H : |: fo had soamnure exeostne AR Eh 2 DISSOLVEDOXYGENCONCENTRATIONS, mot. E \ TesrSbemnecte aa [107 TREMNUTES | ot[0 Toms astooC riloiz1iA aElAE sglia1sPF PlFFeRT dEaLl 103 -_=amc. coamEE |FBa] 1 l imr EYEE a 19.84 ETTeERE gm o" mmEEEEedaiE hh BE aal eonsoomm eeP11Y50 17050%.51 [7 =(Ooi OeRnO sa S OB 3775|cor [wre ica yeos sens | 20% He oRsAoian,rtomsOonSa |W G1i2s|i ||E5110 Roo | SOUWs =| 2A]|| 50) || NE | | |W 0es |F|GGonOsd |145S8> | 1747S 4 |S J FEP SREN SNiDU Ss1 | 5 0S5|SAO |S | 0 OS| BSi 0|Se1 EI P 0 P 0 OO OF TO li =a a Cr fats BS --_-- 000312 g HEH Si RT TTRI IRETSHA R LABCo LE iG } 252 8 Ee r RHEEpHi k TEER pIee: i#Fe 1iE Hgbeil | m ey Eo 0 iIPoEa [||H= HEBE 28 [eReEl g e Ath { ] EEEE: i ll he |om dllil Hliet i L4 ik8H. i FE J Sif = 4 . py S87 j - BEST COPY AVAILABLE : = ENVIRNOMENTAL LABORATORY wn Lracsfacsy 3 poes (OECD ACTIVTATeEsD tSsLoUlDuGEToRNESSPPIRREAPTAIROANTIIONHNIBITIONTEST #200 ton swuance: 35-Dublocoghunal Refeeence Subodance ) fo samo pescrpion: Alles, Chusniedl Co Red Label Let couronz. Eo ayeGWT SEH oun: _ajudfas sh ge eJrock m eSolutren gal Lal nC Bs (FAEEEEBTIn mu Se ui $s al=ien allied : FLies LO Et MlaStein wata tle pldbpe odntprDeaopibia Th be l Eames mtn rere is TTeeco e B8rdaele geait B Ho GT CBEe miLasiod mlLMaMl"))vwear| nso| rr ol Vtumo -- Ty 2st og ugmE sell 2Z0o6c ARO SSCeOs wl Erle CHEROIERIL ne 5 TR SCh o A seE on R eGSA eaD ee cT rRo Esi ox SERRA Ea aeRO ie NS TE AE ARN intCr RP A Emm. =osama] i a ed I ee i ucET O A Rs SE Rs Tio Yue I] || 000314 Css - nt OU AVAILAD 7a Gof 7 5 . ENVIRONMENTAL LABORATORY EEREE Fk E OECD ACTIVATED SLUDGE RESPIRATION INHIBITION TEST #209. ' RAW DATA Bhi rou svar: 3,5Decllecs drionl Ribioses Shue 00 k } ores of Acted use wines Uns dbl (0 Touran NSISLLY DateReceived: 9/4 Jos a brass Copa ate Usea: [14/75 an. E Eso Shen = umure exvosuie| 1 ose onancovcomanomst. (1 | rasa Ta a, [ETE i] Tew Tip neta et at JPR 5 a 1 FGAWo 173 ome a a Ae llel(e Sr s Epi .sammssBEE i esee os aTros, ro ROUE LE Substance S| 25 al Tra A AA a a BE | Temp =i Ci a5n0ppyA EEnD s7n 0 Ai 10T Fog Oi R OD |P5 w5 pennantCS a |se oe osmnws csi hoot oi SOS Gs | ol wer ELT F503 ies 0 GIST|S 0 0 70 ums A | AL CRO IE Tp stn SZ ] 000315 ee LLIB EE BEST COPY AVAILABLE iHnEa TSEITTTTECHEREY SGeTr TTHEEkEe:e | 5iadd HHHEEETIICTEEERR N{TEIREE SBS vo HETIIRREEEL EBTITUIEEEEEEL 3Fg | bPoOOWRITEEEERE REITTTREER Co do RIITEEEE RITTER RITRRE, BITTER TT) TTTPBR ITE: iby|ELITEREE al aed) BARE Le fe Eo ETE a ae a i LRILR TINE--. EEE TC o ANAS ER EET PRERaesoa BRC ade ) i 000316 MHHERE EERHT R 1 Ili4!N T CTTT e T e ee | i g; ES Te T [Berea REH E8 |8 ERT eee eer) EEE Ee - | 2 151s Z3o08 Re ER aE gi at Mg ERS 4 a 4 a & gel hie : Fd Fr