Document Q65nQXwwJeZMoON2z2JmDv0E

BACK TO MAIN ACUTE TOXICITY TO AQUATIC INVERTEBRATES (DAPHNIA MAGNA) TEST SUBSTANCE_________________________________________ Identity: Perfluorooctanoic acid, ammonium salt; may also be referred to as PFOA ammonium salt, Ammonium perfluorooctanoate, PFO, FC-116, FC-126, FC-169, or FC-143. (Octanoic acid, pentadecafluoro-, ammonium salt, CAS # 3825-26-1) Remarks: The 3M production lot number was 427. The test sample is FC-143, referred to by the test laboratory as N2803-4. The T.R. Wilbury study number is 895-TH. The purity of the sample was not sufficiently characterized, although current information indicates it is a mixture of 96.5 - 100% test substance and 0 - 3.5% C6 , C7, and C9 perfluoro analogue compounds. METHOD Method: U.S. EPA-TSCA Guideline 797.1300 Test type: Acute static GLP: Yes Year Completed: 1995 Species: Daphnia magna Supplier: Obtained from cultures maintained by T.R. Wilbury Laboratories Inc, Marblehead, MA from an original culture from Aquatic Research Organisms, Hampton, NH. Analytical monitoring: DO, conductivity, pH, and temperature Exposure period: 48-hours Test organism age: < 24-hours Statistical methods: Interpreted by standard statistical techniques. Computer methods (Stephan, 1983) were used to calculate LC50s and EC5os. Test conditions Dilution water: Deionized water adjusted to a hardness of 160-180 mg/L as CaCO3/L Dilution water chemistry: Hardness: 164 mg/L as CaCO3 Alkalinity: 106 mg/L as CaCO3 TOC: < 1.0 mg/L Residual chlorine: <0.1 mg/L Lighting: Cool-white fluorescent bulbs with an intensity of 110 ft-c. Photoperiod of 16-hours light, 8 -hours dark with a 15 minute transition period. BACK TO MAIN Stock and test solutions preparation: A 1,000 mg/L primary stock solution was prepared in dilution water. After mixing, the primary stock was proportionally diluted with dilution water to prepare the test concentrations. No insoluble material was noted during the test. Exposure vessels: 300 mL glass beakers containing 250 mL of test solution. The approximate depth of test solution was 9 cm. Number of replicates: 2 Number of daphnids per replicate: 10 Number of concentrations: five plus a negative control Water chemistry during the study: Dissolved oxygen range (0 - 48 hours): 8.4 - 8.7 mg/L (control exposure) 8.1 - 8.7 mg/L (1,000 mg/L exposure) Conductivity range (0 - 48 hours) 540 - 560 gmhos/cm (control exposure) 710 - 720 gmhos/cm (1,000 mg/L exposure) pH range (0 - 48 hours) 8.0 - 8.3 (control exposure) 8.0 - 8.2 (1,000 mg/L exposure) Test temperature range (0 - 48 hours) 19.1 - 20.5C (control exposure) 19.4 - 20.5C (1,000 mg/L exposure) RESULTS Element basis: mortality and immobilization Nominal concentrations: Blank control, 130, 220, 360, 600, 1,000 mg/L. Element value and 95% confidence interval: 24-hour EC50 = 780 (600 - 1,000) mg/L 24-hour LC50 = >1,000 mg/L (C.I. not calculable) 48-hour EC50 = 720 (600 - 1,000) mg/L 48-hour LC50 = 720 (600 - 1,000) mg/L 48-hour NOEC = 360 mg/L Element values based on nominal concentrations. BACK TO MAIN Cumulative percent immobilization (includes mortality) Nominal Test Conc., mg/L 24-hours 48-hours Control 130 220 360 600 1000 0 0 0 0 0 100 0 0 0 0 15 100 Note: At 1000 mg/L, all daphnids were immobilized at 24 hours and dead at 48 hours. Control response: satisfactory CONCLUSIONS The test substance 48-hour LC50 for Daphnia magna was determined to be 720 mg/L with a 95% confidence interval of 600 - 1,000 mg/L. The test substance 48-hour EC50 for Daphnia magna was also determined to be 720 mg/L with a 95% confidence interval of 600 - 1,000 mg/L. The 48hour no observed effect concentration (NOEC) was 360 mg/L. Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY Reliability: Klimisch ranking 2. The study lacks analytical measurement of test substance concentrations in the test solutions and sample purity is not sufficiently characterized. REFERENCES__________________________________________________ This study was conducted at T.R. Wilbury Laboratories, Inc., Marblehead, MA, at the request of the 3M Company, Lab Request number N2803-4 OTHER_________________________________________________________ Last changed: 4/01/01