Document Q0Ek3LD3pV8D5Y79q6dQdvD7

ACUTE TOXICITY TO AQUATIC INVERTEBRATES TEST SUBSTANCE (DAPHNIA MAGNA) Identity:A mixturecontainingperfluorooctanesulfonatweh,ich may alsobe referredto as PFOS, FC-95, or as a component of FC-206CE. (1Octanesulfoniaccid)(CAS # 2795-39-3). Remarks: The 3M productionlotnumber was 502. The testsample isFC206CE (2/16/89).Currentinformatioinndicatesitisa mixtureof0.92% PFOS, 15% diethyleneglycolbutylether,76.09% water,4% urea,1.28% Sultonefoamer, 1.55% sodium oetylsulfate,1.05% polyoxyethylene monooctylphenylether,0.06% sodium laurylsulfatea,nd 0.05% tolytriazole. The followingsummary appliesto a mixture with incompletely characterizedconcentrations of impurities.Data may not accurately reflectthe toxicityof the fluorochemical component of the testsample. METHOD: Method: OECD Method 202 and U.S. EPA Method EG-1 Type: Acute static GLP: No Year completed: 1989 Species: Daphnia magna Supplier:In-houseculturesI.nitibarlood stockfromAquaticBioSystems, Inc,FortCollins,CO. Analyticalmonitoring: Temperature, pH, and DO Exposure period: 48-hours Test organism age: <24 hours old Statisticamlethod: Not given. Test conditions: Dilutionwater: Carbon-filterewdellwater Dilutionwater chemistry: Temperature: Dissolved oxygen: pH: 20 OC 9.6 mg/L 8.0 Lighting: Not given. Stock and testsolution preparation: The testmaterialwas added directly tothedilutiownater on a weighvvolume basis. Exposure vessels: 250 mL Pyrex glassbeakers containing200 mL oftest solutionand a waterdepth of6 cm. Number of replicates:2 Number of daphnids per replicate:10 Number of concentrations: one plusa blankcontrol Water chemistry during the study: pH range(0-48hours): 8.0- 8.2(contreoxlposure) 7.9- 8.1(1000 mg/L exposure) Temperature range (0-48hours): 20 -21 *C Dissolved oxygen range (0-48hours): 8.5- 9.6mg/L (controelxposure) 4.5- 9.6mg/L (1000 mg/L exposure) RESULTS Nominal concentrations:Blankcontroland 1000 mg/L Element values: 48-hour EC50 = >1,000 mg/L Element valuesbased on nominalconcentrations Remarks: Testingwas conducted on a mixtureas describedinthe Test Substance Remarks field.The valuesreportedapplytothatmixtureand not the fluorochemicalproportionalone. CONCLUSIONS The FC-206CE 48-hour EC50 was determined to be >1000 mg/L. Submifter: 3M Company, EnvironmentalLaboratory,P.O. Box 33331, St. Paul,Minnesota,55133 DATA QUALITY ReliabilityK:limischranking2. Testingmeets the criterifaorqualitytesbng. However, sample puritywas notproperlycharacterizedand the studylacks analyticaclonfirmationofthe amount offluorochemigalproportioninthe solution. REFERENCES This studywas conducted by the 3M Company, EnvironmentalLaboratory, St.Paul,MN, Lab Request number Gl 358-2,1989. OTHER Last changed: 6/26/00 LR EWIR0,14ENTAL LABORATORY DAPHNIA TOXICITY DATA SHEE7 TEST TYPE-A_IR14_ &la?@. CHEMICAL TESTEI)-.@ Yl)lo ORGANI%F I ra--SaJRCE I'@ AGE OF TEST ORGANI,%fS:424 hrs_ old inal 54%AWSAr,LYA//,;W@ /7,DATE REC' (neonates),NO. ORGANI94S PER IMIHW_/o TEST VESSEL: 250 ml PylexR glass beake_r TEST SOUITION VOLTM: 200 ml WATER DEPITI:.A TIME DIU]TION IVATER Carbon-fil-t- DATE STARTM)--S,&,@*_ DATE C31PLETED r//,-3/x 9 well water (Temp._,Za__? C,, mg/L '- pfi Y,n ZU<_ ECSO(951 Confidence Limits) 169n digli Test Vessel No. Initial Conc. mg/L Total Amotumn Added 24 Hours Total No. pH Dead D.O. mg/L 48 Hours otal No, pH Dead D.O. mS/L -T Control rnL44m I IrAo Im 0 -Test Temp. OC Initials & Date Ccmments: U 3 -5 lyti+iAD, 4 4 inr)nmqii Test methodology conforms with the following two test protocols: (1) OECD #202 "Daphnia sp., Acute Imobilization Test and Reproduction Test". (2) US-EPA #BG-1 "Daphnid-Acute Toxicity Test".