Document OzVwXZQnNb2ZXp0n5zp9mRo8M
ACUTE TOXICITY TO AQUATIC INVERTEBRATES (E.G.,DAPHNIA)
TEST SUBSTANCE
Identity:Perfluorooctylsulfonadtied,ecyldimethylammonium salt;may alsobe referredtoas Fluoroalkyalmmonium derivative[.1Decaminium, N-decyl-N,N-dimethyl-s,altwith 1,1,2,2,3,3,4,4,5,5,6,6,7,7,8,8,8-heptadecafl-uoro-1 octanesulfoniaccid (1:1)C,AS # 251099-16-8]
Remarks: The 3M productionlotnumber was Lot 1. The testsample is L-14394 referredto by the testlaboratoryas P3025. The sample was labeledF-11615, Lot 1. The testsample isa mixtureofthe testsubstance inwater (approximately30-40% testsubstance,60-70% water,and 0-5% ofresidualperfluorochemicals)A.llvalues reportedrelateto thismixture. The testsample appears tobe a 2-phase dispersion(clearliquiwdith opaque solid)which rapidlyseparatesafteragitation.No calculations were made to adjustforthe actualconcentrationofthe testsubstance in the testsample.
METHOD
Method: OECD 202 Test type: Staticacute GLP: No Year Completed: 1996 Species: Daphnia magna Analyticalmonitoring:DO, pH, temperatureand conductivitwyere monitored daily. Statisticamlethods: EL5o values calculatedusing Trimmed SpearmanKarber method. NOEL value calculatedusing Steel'sMany-One Rank test. Test daphnid source: Obtained from culturesmaintained by AScl Corporation,Duluth,MN. Test daphnid age at study initiation<: 24-hours
Test conditions
Dilutionwater: DechlorinatedCityof Duluth,MN tap water. Water
was aeratedfor24-hourspriorto use inthe test.
Dilutionwater chemistry:
hardness: 44 mg/L as CaC03
pH:
8.04
LightingC:ool-whitfeluorescebnutlbs.Photopedodof16-hours light8,-hoursdark. No transitiopneriodnoted.
Stock and testsolutionspreparation: Water-accommodated fractionsT.est solutionswere prepared individualfloyreach concentrationby mass additionofvigorouslyshaken testsubstance in1 L ofdilutiownater. The solutionswere vigorouslystirrefdor23hours (vortex1/2to 1/3solutiondepth).The aqueous phase was siphoned from thevesselatmid-depth aftersettlinfgor1-hour. Exposure vessels:250 mL borosilicatgelassbeakers containing 200 mL oftestsolution.The solutionswere kept coveredduring the test. Number of replicates:Four Number of daphnids per replicate:Five Number of concentrations:fiveplusa negativecontrol Water chemistry during the study:
Dissolved oxygen range (0- 48 hours): 8.6- 9.1mg/L (controelxposure) 8.0- 8.8mg/L (50mgll exposure)
pH range (0- 48 hours) 8.04-8.11 (controelxposure) 7.92- 8.00 (50mg/L exposure)
Test temperature range (0- 48 hours) 20.9 - 21.OOC
Conductivity range (0- 48 hours): 142 - 155 pmhos/cm (controelxposure) 120 - 124 pmhos/cm (50 mg/L exposure)
Element basis:mortalitaynd immobilization
RESULTS
Nominal mg/L Element
loading concentrations: Bk control,3.13,6.25,12.5,25,50
value: 24-hour EL50 = 27.0 (18.7-39.0)mg/L 48-hour EL50 = 11.3(9.6-13.2m)g/L 48-hour NOEL = 6.25 mg/L
Allelement valuesbased on nominal concentrations.
StatisticaElvaluation:The EL50 valuesand 95% confidenceintervals were calculatedby the Trimmed Spearman-Karber method. The NOEL was calculatedusingSteel'sMany-One Rank testusingthe TOXSTAT statisticsaolftwareVersion 3.2,UniversitoyfWyoming.
BiologicaolbservationsD:aphnidsinthenegativceontroaln,d the3.13 and 6.25 mg/L treatmentsappeared healthyand normal throughoutthe testwithno mortalityi,mmobilitoyr overtclinicaslignsof toxicityT.he effectsnoted inthisstudywere mortalityn;o immobilizatiownas noted at any testconcentration.The number ofsurvivingorganisms were determined visuallaynd recordedinitialalnyd after24 and 48 hours.
Cumulative percent m rtality:
Nominal Loading Test Conc.,mg/L
24-hours
48-hours
Neg. Control
0
0
3.13
0
0
6.25
0
0
12.5
5
70
25
50
95
Control response: satisfactory
Remarks: Values reportedare forthe testsample. No calculationwsere made toadjustfortheconcentrationofthe testsubstance inthe test sample.
CONCLUSIONS
The testsubstance 48-hour EL50 forDaphnia magna was determined to be 11.3 mg/L witha 95% confidenceintervaolf9.6-13.2mg/L. The 48hour no observed effectlevel(NOEL) was 6.25 mg/L.
Submitter:3M Company, EnvironmentalLaboratory,P.O. Box 33331, St. Paul,Minnesota,55133
DATA QUALITY
ReliabilityK:limischranking2. The studylacksanalyticamleasurement oftestsubstanceconcentrationsinthe testsolutionsand sample purityis not sufficientclhyaracterizedA.dditionallyd,ata isfora mixtureand toxicitcyannot be positivelayttributetdodidecyldimethylammonium Perfluorooctylsulfonastaeltalone.
REFERENCES
This studywas conducted atAScl Corporation,EnvironmentalTesting DivisionD,uluth,MN, attherequestofthe 3M Company.
OTHER
Last changed: 5/24/00
ASciCorpomlion]AScl-Duluth EnvimntnertTteAsLtIinDgivision
ASciStudyID# 5010-069
STUI)Y TITLE
ACUTE TOXICITY OF
DEVELOPMENTAL
TO DAPHNL4 MAGN.4
MATERIAL
DATASTANDARD OECD GUIDELINF- 202
STUDY COh4ELETED January 10, 1997
TESTING FACILITY
ASCI Corporation/ASel-Duluth EnvironmentalTestingDivision
4444 AirparkBoulevard Duluth,MN 55811
Tel.No. (218)722-4040 Fax No. (218)722-2592
STUDY IDENTIFICATION NUMBERS ASel Study ID# 5010-068
3M Company Lab Request# P3025
Sponsor: 3M Company Sponsor StudyID# P3025
ASci Co rpomtion/AScl-Duluth Environmental TestingDivision
ASci Study ID# 5olo-o68
1.0 GENERAL MORMATION
StudyTitle Data Standard Sponsor
Sponsor'sRepresentative
Sponsor'sLab Request# TestingFacility
PrincipalInvestigator ProjectDirector TestingFacilitDyirector DefinitiveTest Dates Data Validationand ReportReview
AcuteToxicitoyf
Developmental
MaterialtoDaphnia magna.
OECD Guideline202.
3M EnvironmentalTechnology and Safety Services,Building2-3E-09,935 Bush Avenue, St.Paul,MN 55133.
Susan A. Beach, 3M Environmental Technology and SafetyServices,Building23E-09,935 Bush Avenue, St.Paul,MN 55133; Tel No. (612)778-7452.
P3025.
AScl Corporation/AScl-DulutEhnvironmenL-d TestingDivision,4444 AirparkBoulevard, Duluth,MN 55811;Tel.No. (218)722-4040.
Joe Dierkes.
Joe Amato.
Donald Mount.
December 11-13,1996.
Alan Mozol.
Re rtsignature:
Retentionof Raw Data and FinalReport Locationof Raw Data and FinalReport
Two yearsfrom thedatestudyiscompleted.
ASCI Corporation/AScl-DulutEhnvironmental TestingDivision,4444 AirparkBoulevard, Duluth,MN 55811;Tel.No. (218)722-4040.--Jl
Sponsor: 3M Company
Sponsor Study ID# P3025
2
ASci Corporation/ASIc-Duluth Environmental Testing Division
ASci Study ID# 5010-068
2.0OBJECTIVE(S)
To determintehe48-hELSO(effectliovaedinga)nd48-hNOEL (noobserveedffecltoadingo)f thetesstubstancLe-,14394DevelopmentMaalteriaflo,rDaphniamagna.
Thisstudywas conducteadccordintgotheASCIstudyplanretainbeyd theSponsoarndOECD Guideline203 (OECD 1993). The testsolutionwsere water-accommodatedfraction(sWAF) preparedfollowingGirlingW,hale, & Adema 1994.
3.0 TEST METHODS
TestSubstance Test Organisms DilutionWater
Propenies:f@c)@@ two-phaseliquidbeforeagitatedl,ightcolored,nearly odorlessslurryafteragitations,pecifigcravityca.1.2g/cc,and insoluble in water.
Testloadings:0 (dilutiownater control)3,.13 mg/L, 6.25 mg/L, 12.5 mg/L, 25 mg/L, and 50 mg/L. Testloadingsolutionwsere prepared individuallfyoreach concentration.The solutionwsere preparedby mass additionof vigorouslyshaken testsubstancein 1 L of dilutiownater. The solutionwsere vigorouslystirre(dvortex1/3to 1/2solutiodnepth)for 23 h. The aqueous phase was siphonedfrom thevesselatmid depth.
Source: .Healthy,lessthan 24-h old Daphnia magna neonatesobtained from AScl stockculturesmaintainedinwaterof testdilutiownaterquality.
Use intest:Gravidadultsisolatedin dilutiownater24 h beforethetest initiationU.sed 5 organisms/replicaatned 4 replicates/tceosntcentration. Handled witha smooth bore pipetwithan insidediameterof 6 mm. Did not feedduringthetest.
Source: DechlorinateCdityof Duluth,MN, tapwater. The most recent chemicalanalysisisinRaw Data Package.
CharacteristicsA:t testtime,the water had a hardnessof 44 mg/L (as CaC03) and a pH of 8.04. Aeratedfor24 h priortousinginthetes
Sponsor: 3M Company
SponsorStudy ID# P3025
3
ASel Corporation/AS[c-Duluth Environmental Testing Division
ASci Study [D# 5010-o6a
Exposure Chambers Incubation
Observations
Endpoint Calculations
7@pe:250-mlborosilicgaltaesbseakers.
Use intest: 200 ml of dilutionwater or testsolution/chamber.During the test,kept covered,exceptwhen experimentalobservationswere made. Duration:48 h.
Dailyphotoperiod: 16 h lightand 8 h dark,maintainedusingcool-white fluorescenbtulbs.
Temperature: 21*C. Biological:Daily- gentlyagitatedtheexposurechambers and thencounted immobilizedand dead testorganisms(immobilizedorganismswere those thatdid not swim within15 secondsafterexposurechambers were gently agitated).Did not remove any daphnidsfrom theexposurechambers at 24 h.
Water chemistry:(1)At testinitiatiotno-talhardnessand alkalinity(,2) daily-temperaturep,H, DO, and conductivityA.lldeterminationwsere made accordingtoAPHA methods (1995). ELSO: Trimmed Spearman-Karbermethod.
NOEL: Steel'sMany-One Rank Test (TOXSTAT statisticsaolftware, Version3.2,Universitoyf Wyoming).
Sponsor: 3M Company
Sponsor Study ID# P3025
4
ASciCorpomtion/ASci-Duluth EnvironmentTaclstinDgivision
AScl StudyID# 5010-06S
3.0RESULTS
TestSubstancTeoxicit(yPertinednattaarein Table 1)
Ranges of Water Chemistry Parameters
24-hEL50:27.0mg/L (18.7-39m.g0/L). 48-h EL50: 11.3 mg/L (9.6-13.2mg/L). 48-h NOEL: 6.25 mg/L. Totalhardness (asCaC03 mgll): 41-44. Alkalinity(asCaC03 mgll): 42-45. Temperature (*C): 20.9-21.0*C. DO (mglL): 7.9-9.1. pH: 7.92-8.11. Specificonducti@@ (,gmhoslcm):120-155.
4.0 TEST QA/QC
-QA/QC Criteria
Test organism dead,immobilizedor trappedin controlexposuremust not be more than 10%.
The lowestDO concentratioanttest terminatiomnust be atleast60% of theair saturatiovnalueat testtemperature.
Test durationmust be 48 h.
Referencesubstancetoxicittyest (thistestis optional).
Data 0% dead,immobilizedor trapped.
The lowestDO concentratiomneasured was 89% of theairsaturatiovnalueat21.0"C.
The testdurationwas 48 h. CurrentcontrolchartforD. magna acute testingisincludedin the raw datapackage.
Sponsor: 3M Company
Sponsor Study [D# P30,-15
5
ASci CorporationlAScl-Duluth EnvironmentTaelstinDgivision
ASciStudyID# 5010-06g
5.0REFERENCES
AmericanPublicHealthAssociati(oAnPHA).1995.StandarMdethodsfortheExaminationf Water and Wastewater.APHA, Washington,D.C.
Girling,A.E., G.F. Whale, and D.M.M. Adema. 1994.A GuidelineSupplementfor DeterminingtheAquaticToxicityof PoorlyWater-SolubleComplex MixturesUsing WaterAccommodated FractionsC.hemosphere 29(12):2645-2649.
Hamilton,M.A., R.C. Russo,and R.V. Thurston.1977.Trimmed Spearman-KarberMethod for EstimatingMedian LethalConcentrationisn ToxicityBioassays.Environ.Sci.Technol. 11(7):714-719C;orrectio1n2(4):417(1978).
OrganizationforEconomic Cooperationand Development (OECD). 1993.OECD Guidelinesfor Tes ng 0 Chemicals.OECD PublicatioInnformationCenter,Washington,D.C.
Universityof Wyoming. ToxstatVersion3.2.Cheyenne, Wyoming 82001.
Sponsor: 3M Company
Sponsor Study ID# P30'25
6
ASci Corporation/ASci-Duluth Environmental Testing Division
AScl Study ID# 5010-@8
TABLE 1. ACUTE TOXICITY OF P'@c4 DEVELOPMENTAL MAGNA
MATERIAL TO DAPHNIA
mg/L) 0
3.13 6.25 12.5 25
50
T
C=Wative' Number and Percentof AffectedOrganisn3s'
24. h
48 h
0(0) 0(0) 0(0) 1(5) 10(50) 15(75)
0(0) 0(0) 0(0) 14(70) 19(95) 20(100)
a Percentagesare in parenthesis.
EFFECT AND NO-EF7ECT LOADINGS WITH
CORRESPONDING 95% CONFIDENCE INTERVALS
on
24-h EL50 48-h F-Lso 48-h NOEL
lba't'i
27.0
11.3 6.25--
95 % Confidence Interval 18.7-39.0 9.6-13.2
Not Applicable
Sponsor: 3M Company
Sponsor Study ID# P3025
7