Document Oz7a2ZaY4jgxERDx312qZ8BDX
ACUTE TOXICITY TO FISH
TEST SUBSTANCE
Identity: Perfluorodecanesulfonate, ammonium salt; may also be referred to as PFDS, FC-121-X, or3M Sample E2112and E2566-1. (1Decanesulfonic acid, 1,1,2,2,3,3,4,4,5,5,6,6,7,7,8,8,9,9,10,10,10-heneicosafluoro-, ammonium salt, CAS # 67906-42-7)
Remarks: Test sample is a mixture of the test substance in water and 2propanol, 1-(1,1-dimethylethoxy). This mixture contains approximately 2226% test substance, 33-37% propanol, 1-(1,1-dimethylethoxy), 0.5-1.5% perfluorononanesulfonate, ammonium salt and 38-42% water). All values reported relate to this mixture. No calculations were made to adjust for the actual concentration of the test substance in the test sample. Test material was from 3M production lot 1.
Note: Only the summary pages of this report were available.
METHOD
Method: OECD 203 Type: Static acute GLP: Not known Year completed: 1988 Species: Pimephales promelas Supplier: ABC Laboratories In-house cultures, Columbia, MO Analytical monitoring: Dissolved oxygen, temperature and pH. Test substance concentration was not measured. Exposure period: 96-hours Statistical methods:Binomial method. Length and weight: Average length = 18 mm.
Average weight = 0.087 g
Test conditions: Dilution water chemistry: hardness: alkalinity: pH:
44 mg/L 30 mg/L 7.5
Stock and test solution preparation: Details not available. All test vessels containing FC-121 contained a thin layer of foam at 0-
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hours. The amount of foam increased with the concentration. No foam or film was present after 24-hours. Number of fish per concentration: ten Number of concentrations: five plus a negative control Water chemistry during the study: within acceptable limits throughout the study. Temperature during the study: 22 + 1C
RESULTS
Nominal concentrations: Bk control, 3.2, 5.6, 10, 18, and 32 mg/L Element value: 24-hour LC5o = 26 (18-32) mg/L
48-hour LC5o= 24 (18-32) mg/L 96-hour LC5o= 13 (10-18) mg/L 96-hour NOEL = 3.2 mg/L All element values based on nominal concentrations
Statistical Evaluation of Mortality: Binomial method used to calculate LC50 values and corresponding confidence intervals.
CONCLUSIONS
The test sample 96-hour LC50for fathead minnow was determined to be 13 mg/L with a 95% confidence interval of 10 - 18 mg/L.
Testing was conducted on the "as-sold" mixture of the subject material in water (-22-26% subject material. The values reported apply to that mixture and not directly to the subject material.
Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133
DATA QUALITY
Reliability: Klimisch ranking 4. A portion of the final report is missing. Because a mixture was tested, care must be taken when interpreting the results. The solvent in the mixture could have enhanced or restricted biological uptake and/or toxicity of the fluorochemical component. Characterization of the test substance purity and analytical measurement of tested concentrations was lacking.
REFERENCES
This study was conducted by ABC Laboratories, Columbia, MO on behalf of the 3M Company.
OTHER Last changed: 5/18/00
0001-84
SPONSOR
3M Company Attn: Mr. Mohamed Elnabaravy
EE & PC Environmental Labs Building 21-2W-05, 900 Bush Avenue
St. Paul, Minnesota 55106
STUDY TITLE
Acute Toxicity o: E2566-1 to Fathead Minnow (Picephales procelas)
DATA REQUIREMENT OECD Guideline *2('3
Ai"; noa
Jane l. Bowman Biologist II
STUDY COMPLETED ON January 15, 19BS
PERFORMING LABORATORY
Analytical Bio-Chemistry Laboratories, Inc, Aquatic Toxicology Division 7200 East ABC Lane P.0. Box 1097 Columbia, Missouri 65205
ABC LABORATORY PROJECT ID #36615
Page 1 of 62
BEST COPY AVAILABLE
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Submitted By:
Analytical Bio-Chemistry Laboratories, 7200 East ABC Lane P. 0. Box 1097 Columbia, Missouri 65205 (314) 474-8579
Inc.
Prepared By:
M >- JAne H. Bowman Biologist II/Study Director
ii Laura Mullen Biologist I
^ ' -',v' Date
Date
Approved By:
l-j. nes P. Swigert. Ph t. Supervisor, Fish Acute/ Chronic Investigations
Phillip M. Buckler Quality Assurance Supervisor
Date
0$
Date
:dab31
Carl M, Thompson'' Aquatic Toxicology Manager
0001.86
Date
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. *i ABC LABS #36615-2 of 62
FISH ACUTF TOXICITY COMPENDIUM
Subject: "Acute Toxicity ol EL5fe6-l to Fathead Minnow.' (Piaephales proaelaa) A B C Study Number 3t>615.
Sponsor: 3M Company
Testing Facility:
Analytical Bio-Chewistry Laboratories, Inc. Aquatic Toxicology Division P.0. Box 109? Columbia, Missouri 65205 3:4-474-8579)
Location of Original Raw Data anc Fin;; Report;
3M Company St. Paul, Minnesota SSJUf
Test Material: E2566-1
Test Concentrations: Control, 3.1, 5.6, 0. W and 32 ag.'I
Dilution Water: 44 mg/1 (hardness), 30 mg/1 (alka'iaitv), 7.5 (pH)
Test Dates:
Initiation - January li, 1988 Termination - January 35, 1988
Length oi Study: 96-hours Results: 96-hour LC5{) - 13 g/l (95X C.3. - 10-18 ag/l)
96-hour NOEL 3.2 mg/1 96-hour Slope of the Dose-Response Line was 32
, Teat Species: Fathead Minnow (Plctephales proaelas)
Source of Organisms:. ABC Laboratories in-house culture
`'jMean Weight: 0.087 g (10.027 g) -Mean Length: 18 nun (il.7 mo)
0 0 0 .1 B 7
ABC LABS *36615-6 of 62
SUMMARY
The .1.ute toxic ity of E2566-1 to fathead minnow (Pinephales prone la* ) was assessed using the methods outlined by the Committee on Methods tor Toxicity Tests with Aquatic Organisms. Water quality pataactr* of temperature, dissolved oxygen and pH were measured throughout the* rest and were within acceptable Unit s . Culture and icc lim.it tov records indicated the tish were in good condition for tent ing.
?;: tudv was conducted at the following nominal concentrations of 12 Vjh-;; j.2, 5.6, 10, 13 and 32 og/i. A thin layer of foam was present In all chambers at 0-hour. The amount cf foam increased with the c ncontratlon. No foam or film was present after 24 hours. Ten fish, with a mean weight of 0.087 (*0.027) g and a mean standard length
i 18 .1 1.\s mm, were exposed to each test concentration and control.
.;i results vt the four day static firh toxicity study using
> >V'- . ..i; uiraaarised below. The 24-, 48- ar.d 72-hour LC,{) values were
ill-, u.
red. The '36-hour slope of the dc.se response line was 32.
: ,-nrbund
--------A -- -- -- . . .
K2!>sh- i
bb-hcor 13 sg/l
n 0 - 1 5 ag/l)
Also, the results indicated a 9fc-hcur, r.o-cb served effect concen tration could be estimated at 3.2 mg/1, which was based cn the lack of mortality and abnormal effects. Abnormal effects of mortality, loss of equilibrium, light discoloration and/or fish on the bottom of test chamber were observed during the 96-hour exposure period in the 5.6, 18 and 32 mg/I test concentrations.
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TABLE 2
Acute Toxicity of E2566-1 to Fathead Minnow (Pinephales pronelas)
Compound E2566-13
in milligrams/liter (ppm> LC50
24 hours
48 hours
96 hours
26 '
24<l>
i3(l)
v18-32
(lS-22)b
(iu-;8)b
N * 10 fish per concentration.
a Bioa5say as conducted at 22C (1.0), mean fish weight and length, 0.087 (0.027) g and 18 (1.7) no.
b95Z confidence Units.
The 96-hour no-observed effect concentration could be estimated at 3.2 mg/1, based on the lack of mortality and abnormal effects at the lowest concentration tested.
M ' 1, ,,i t>
. iAc 1'b ' A. *'w C V ' f $
l l ' Si, }Sf
The slope of the 96-hour dose response curve as calculated by least squares regression analysis was 32.
All values were rounded to two significant figures following ABC S.O.P.
#8.7.
LCj q calculated using: /1\ Binomial Method
Moving Average Method
Probit Method
*1 ^ * i t'*J
t'
* *r f (<*
<-'00X^9
a 'CSSS''
J5J
ABC LABS #36615-12 of 62
