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PLAINTIFF'S EXHIBIT
(412) 607-2100
INDUSTRIAL HYGIENE FOUNDATION OF AMERICA. INC.
5231 CENTRE AVENUE
PITTSBURGH. PA. 15232
PROGRESS REPORT NO. 4 on
FIBROUS DUST STUDIES
for Johns-Manville Corporation Pittsburgh Corning Corporation
PPG Industries Owens-Corning Fiberglas Corporation
Raybestos-Manhattan. Inc.
by Paul Gross. M.D. Director of Research Laboratory
Robert T. P- deTreville. M. D.. D. Sc. President
December, 1968
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INDUSTRIAL HYGIENE FOUNDATION OF AMERICA. INC.
5231 CENTRE AVENUE
PITTSBURGH. PA. 15232
FIBROUS DUST STUDIES REPORT OF PROGRESS COVERING THE QUARTER ENDING DECEMBER. 1968
Experimental studies on rats and hamsters injected intratracheally with fibrous dusts were described in our proposal dated June 1, 1966, and progress reports dated February, 1967; June, 1967; and June, 1968, respectively, have been submitted to sponsors.
Cumulative mortality among a total of 485 rats injected with the types of dust and in the dosages shown, is indicated in Table 1, in the format normally used in technical publications.
A different form of tabulation in the case of hamsters to show percent of mortality by dust type dosage and exposure duration has been developed. It differentiates between cumulative mortality versus sacrifice of survivors at the end of the 24^nontha exposure period, and indicates stage of progress of animals which remain under observation.
In addition to the above studies, work on fibrous dusts sponsored jointly with the Mellon Institute; University of Pittsburgh, Graduate School of Public Health; the Department of Health, Education, and Welfare, Occupational Health
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INDUSTRIAL. HYOICNf FOUNDATION OF AMKRICA. INC.
2.
Program; and in collaboration with the University of Cambridge (British Asbestos Council) have been undertaken. These were described previously in 1HF Medical Series Bulletin. No. 11, "Asbestos Bioeffects Research For Industry." Progress has also been reported in papers before the Foundation's 31st and 32nd Annual Meetings and in publications ("Ferruginous Bodies in Human Lungs, Prevalence at Random Autopsies, " M. D. Utidjian, P. Gross,
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and R. T. P. deTreville, Arch. Environ. Health 17:327-333, Sept. 1968; and "Pulmonary Ferruginous Bodies, Development in Response to Filamentous Dusts and a Method of Isolation and Concentration, " P. Gross, R. T. P. deTreville, L. J. Cralley, J. M. G. Davis, Arch. Path. 85:539-546, May. 1968.)
In addition, the most current report of progress was given at the Foun dation's Fibrous Dust Seminar on November 22, 1968, at Mellon Institute (Enclosure). A report of the proceedings is now in preparation and should appear in 1969; however, it may be of interest to record here that 100% of the cores, of the 17 ferruginous bodies isolated by Dr. Michael D. Utidjian? from the 100
a
autopsy specimens in the Foundation's Laboratory when subjected to electron diffraction by Mellon Institute, were shown not to be chrysotile asbestos. They are crystalline and it is not possible to rule out some complex form of asbestos. However, they do not appear similar to diffraction patterns obtained on known samples of amosite and crocidolite provided to XHF's Research Laboratory by
Dr. James Leinweber, Johns-Manvilie Corporation, Research and Engineering Center, Manville, New Jersey.
Assistant Professor, Department of Epidemiology, University of Pittsburgh, Graduate School of Public Health (formerly IHF Fellow in Occupational Medicine).
INDUSTRIAL HYGIENE FOUNDATION OF AMERICA. INC.
3.
Further progress was reported by Dr. John M. G. Davis, Department
of Pathology, Cambridge University, Cambridge, United Kingdom, who had
presented earlier findings at the Foundation's 32nd Annual Meeting in 1967.
It now appears that the precipitation of a matrix and subsequent impregnation
by iron aound fibrous particles of respirable size is probably a function of scar
tissue formation and availability of iron in the form of red blood cell hematin
from capillaries. In dense scar tissue, every fiber was coated with a matrix
(acid mucopolysaccharide) but few had the invasion by iron granules typical of
ferruginous bodies.
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As the Mellon Institute and U. S. Public Health Service support for the
above studies is ending, a new proposal is being prepared by IHF for a more ex
panded study of fibers and ferruginous bodies from autopsied lungs. Obviously, the above results are on too small a population to allow us to reach any conclusions
but they do represent an area of considerable challenge for further study in our
Laboratory.
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Table 1
Mortality of Rata Injected Intratracheally with Fibroua Dusta
Fibroua Duat
1i
! Glaaa (Goraki)
{ Glaaa (Refraail)
Chryaotile (Elect. Precip.)
Synthetic Chryaotile (Mellon)
Synthetic Chryaotile and
Benzopyrene
Synthetic Chryaotile'and
Nickel
Synthetic Chryaotile and
Chromium
Glaaa and Phenol-Formaldehyde
Binder
Glaaa (uncoated)
Glaaa and Phenol-Formaldehyde
Binder
Brucite
Synthetic Chryaotile and
Nickel
Synthetic Chryaotile and
Chromium
Synthetic Chryaotile and Cobalt
Amorphoua MagneaiumSilicate
and Nickel
Amorphoua Magneaium Silicate
Synthetic Chryaotile and
Manganeae
Talc with low metal content
Talc with high metal content
Brake Drum Dust
Synthetic Chrysotlle (J-M)
Synthetic Chryaotile (J-M) and
Nickel
-
Fiberfrax
Fiberfrax (Hammer milled)
Black Lake Chryaotile (Hammer mill)
TOTAL NUMBER RATS
Doae mg
No. of Rata
Cumulative
Mortality Percenta 8e 6 mo. 12 mo. 18 mo. 24 mo.
3 x 3. 5 3 x 3.5 3 x 3.5 3 x 15
15 15 15 15
3x15 15
3x15 15
3x15 15
3 x 3.5 15 3 x 3.5 15
3 x 3.5 15 4x3.5 15
3x15 10
3x15 3x15
10 10
1 x 25 3 x 25
10 10
3x15 1 x 25 1 x 25 2x10 4x3.5
10 25 25 10 40
4 x 3.5 3 x 3.5 3x3.5 4 x 3.5
40 40 40 40
7 7 13 20
7
7
7
80 13
27 40
30
40 20
50 10
50 44 40
0 12
28 55 52 45
73 100 73 93 93 53 73 100 33 47 53 '
27 40 80
60 73 93
27 40 100
87 87 -- 67 80 --
67 _ mmm 47 47
40 --
50 -- -- 20 -- --
50 -- -- _ ----
50 -- -- ----
22 -- --
35 _ --
58 72 -- 70 85 -- 78 90 --
485
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INDUSTRIAL MTOIENE FOUNDATION OF AMERICA. INC.
Attachment to Table, Percent Mortality--Hamsters
The following dotages were used: A = 3 x 3. 5 mg B = 1 x 3. 5 mg C s 1 x 1.75 mg D = (two dosages were used)
12 = 1 x 3.5 mg 12 = 2 x 1.75 mg E = (two dosages were used) 4 : 3 x 3.5 mg 8 - 6 x 1.75 mg F : 2x 12.5 mg
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