Document OnkVyobq5dNoEb0yJRpgwD5e

Corpor Heath Pris Corporate Health Physics Corporate Occupations Medicioe Corporate Occupational Medicine CPea Corporate Product Responsibility Corporate Toxicology SM datDepartment 3M Medical Department pom 3MCenter,220-2E-02 FO Bor 3220 PO Box 33220 LP e530 St. PauI, MN55133-3220 651 733 III0 oT 3M ``SSCeerLruUumEm IFFIiluu(1oo9rr8oo9cc)hheeemmpiiicdceaamllittorrleeonngddisscooiffnvooeuusttt--ioogffa--tcciooounusnn.ttyy residents residents in in CLUE CLUE I I (1974) (1974) and and CLUE II (1989) epidemiologic investigations. "TThhiiss pprroojjeecctt iissaa joint joint collaboration collaboration between between 3M 3M and and Johns Johns Hopkins Hopkins University University School School of of Public Health researchers. The objtive ofthis study is toanalyze 358 adult sera Public Health researchers. The objective of this study is to analyze 358 adult sera samplesthatwere collected in 1974and 1989as partofthe CLUE I and CLUE Il studies samples that were collected in 1974 and 1989 as part of the CLUE I and CLUE II studies conducted in Washington County, Maryland. The sera samples represent out-of-county conducted in Washington County, Maryland. The sera samples represent out-of-county residents who participatedi the study. A totalof59 samples are from individualswho. residents who participated in the study. A total of 59 samples are from individuals who donated in both 1974 and 1989. An additional total of 120 samples will be analyzed in donated in both 1974 and 1989. An additional total of 120 samples will be analyzed in eeaacchh yyeeaarrooffcdiiffffeerreenntt iinnddiivviidduuaallss.. Fluorochemicaltso be measured include Fluorocherrficals to be measured include perfluorooctanesulfonate (PFO), perluorooctanoate (PFOA), perfluorchexanesulfonate, perfluorooctanesulfonate (PFOS), perfluorooctanoate (PFOA), perfluorohexanesulfonate, Neethyl perfluorooctanesulfonamidoacetate (PFOSAA), N-methyl N-ethyl perfluorooctanesulfonamidoacetate (PFOSAA), N-methyl perfluorooctancsulfonamidoscetate (M570), perluorooctanesalfonamidoacetate (MSS6), perfluorooctanesulfonamidoacetate 0V1570), perfluorooctanesulfonamldoacetate (M556), and perfluorooctanesulfonamide (PFOSA). The timeline for study completion, estimated and perfluorooctanesulfonamide (PFOSA). The thneline for study completion, estimated at January 1, 2001, is entirely dependent upon avalidated analytical methotdo analyze | at January 1, 2001, is entirely dependent upon a validated analytical method to analyze 1 mlofhuman serum for these fluorochemicals witha lowes limitof quantitationof < 10 ml of human serum for these fluorochemicals with a lower limit of quantitation of_< 10 ppb, based on high performance liquid chromatography electrospray tandem mass ppb, based on high performance liquid chromatography electrospray tandem mass spectrometry methods. spectrometry methods. 7172266..00000011 1726 S3MUMMNO0T1666611688833 TSTIeITrTuLLmEEfOOluFFoRrRoEcEhSeSmEEiAAcaRRlCCtHeHnPdPsRRoOOfPPoOuOtSS-oAAfL-Lcounty residents in CLUE 1 (1974) and CLUE Serum CLUE 111989) epidemiologic investigations fluorochemical trends of out-of-county [I (1989) epidemiologic investigations residents in CLUE I (t 974) and I. RREESSAEErAAeRRlCuCoHHrQoQcUhUEemESiSTcTaIlIOOsNNde::tectable in the serum samples of individuals who eddxootnneaanttAteerdddeo bbflllloueovoooeddrlosttcoohceaahmaccnooigcmmeammluosnvudeinertittyeiy-cm-btbaeaab?ssleeeddinsspptheececiismmeeerunnmbbaasnnakkmiipnnle11s99o77f44 and 1989 nd to individuals who and 1989 and to wwhhaatt extent do levels change over time? 2. RRAATT"IITOhOeNN3AAMLLCEEo//BmBApAaCCnKKyGG(RRspOOonUUsNoNrDDo:f: this proposal) manufactures widely-used produTchtse 3tMha Ccoonmtapiannfylu(scprooncshoermiocfatsh.isFpuroorpoocshale)mmicalnsufatrcatunrsefsorwmidmeetlyab-uosleidcally to products that contain fluorochemieals. Fluorochemicals transform metabolically to paenrdfflulusuoeordrooioonecttianandnueesstsuruillfafolonnaaantmdec((oPPmFFmOOeSSr))ciaassalaapnnreeonncddesssttsaaeggsee.mmPeetFatOabbSoloiclitteeo.. PnPFFcOOS$eiiissnnaattsshuuerrrffiaavaccettaartnanttned `auacunnundddmeeuurrlsggeaoodteesisienveneintntetedroruxooishhcteriepitpaayaltrtiaieccncadiclricircocnumulglaamtitniieoorlnnco..iwaelSSrupuebrbdocechsherersoorsnnueiimscc.tssoPtttuuFaddlOiiceeShssociilnonensrracattterssnolaatrnnalddteevppserrliiisnmmataahnttedeesslissvuueggrggaeenssdtt maectuamboulliactiwvaesttoixnigc.itTyhre~sseuletfifnegctisn mloawyebreeddsueerutomeftofetactl schoonleasnteyroalcliedvwealsnsapnodrt, metabolic wasting. These effects may be due to effects on fatty acid transport, m`meettaabboolliissmm,, mmeemmbbrraannee ffuunnccttiioonnss,, aanndd ppeerrooxxiissoommee pprroolliiffeerraattiioonn.. OceupEEaxxtppiooosnsuuarlreelyrreaaxtteepsstotosoettdhheienssdeeiccvhhieedmumiacilcasalhlssaavamemoboneneggtnthhmeeggeoenneenrraallipfpoooptrputulhoaleattpiirrooennaseaerrneedcueunnkoknfnoowwnn.. fOfllucuocoruropoccahhtieeommniiacclaallylss e((xOOpllsoseesnnedeettinaaldl))i..viAAdmumaoolnsnghgammvaeallbeeepperrnooddmuuocctntiiiotoonnreeedmmpfpolloroytyheeeeessprttehhseeemmneceaeannolfleevveellss ooffppeerrfflluuoorroooocettaanneessuullffoonnaattee((PPFFOOSS)) iinn 11999955 aanndd 11999977wweerree22..1199 ppppmm aanndd11..7755ppppmm,, rreessppeeccttiivveellyy.. NNoo ssuubbssttaannttiiaall cchhaannggeess iinn sseerruumm hheeppaattiicc eennzzyymmese,s,cchholoelsetseterrooll,, oorr lipoprotein levels were noted among individual with levels less than pp. Only 7 lipoprotein levels were noted among individuals with levels less than 6 ppm. Only 7 individuals in 1995 and in 1997 had levels in excess of6 pun no marked changes individuals in 1995 and 5 in 1997 had levels in excess of 6 ppm; no marked changes iinn lliippiidd oorr hheeppaattiicc eennzzyymmeess wweerree nnootteedd.. IItt iiss uunnkknnoowwnn ttoo wwhhaatt eexxtteenntt tthheessee lleevveellss can be detectedin the general population. Thisproposal isan explorastdoyrtyo can be detected in the general population. This proposal is an exploratory study to determine or the frst ime whether serum concenotfrflauotroichoeincasls among determine for the first time whether serum concentrations of fluorochemicals among anon-occupationally exposed population increase over timeand whether a non--occupationally exposed population increase over time and.whether concentrations vary by gender or age. concentrations vary by gender or age, 3. MMEETTFHHrOoOmDDASSugust through November, 1974, the Campaign against Cancer and From August through November, 1974, the Campaign against Cancer and `whom 20.305 were county residents. The brief history form was completed at the sSSlttorrogoakknee,ww"aaGssivcceoonnuddsuuaccttCeelddueiinntoWWaCasasnhhciiennrgg"tt)oo,nnaCCtoooutunanltotyyf,, 2MM5DD,.8.02RRepefefeerrrsrroeendds ttdoooaanssatCCeLLdUUblEEooIId((,ffrorofomm tthhee slogan, "Give us a Clue to Cancer"), a total of 25,802 persons donated blood, of imeof bood whom 20,305 time of blood collection (Appendix were county residents. collection (Appendix 1). The Campaign The brief history 1). The Campaign against Cancer and Heart form was completed at the against Cancer and Heart MDDiaissyeeaatssheer((oCuCgLLhUUOEEctIIoIIb))ewwra,ass1s9s6iim9m.iillaarrBrttoioeCCfLLhUiUsEtEorIIieiinsn m(mAoopssptterrneedssippxeecct1ts)s..nIIdtt wwbalasosoccdoonnprddeuusccsttueerddesffrrwooemmre tqMtaaukkeaeesyntn,i,thoaanrnnonddauig22rh00e Omm(Alclpotoofpbfebenblrdlo,oioo1xd9d81ww)9et.eroreeBcddorrrimeaapwfwnlhn.ei.sttaoPParairrethtsiiocc(miiAppeaapnnpattnessndwwdweieexrrreee1gg)aiiavsvnekednendbaaltoffooooroodeddpcffrurerreseqsqiuuureweenniscctyywahere qttooueeennsaatiiilol nccnlliiappiprpeiinn(ggAffpooprrettnrradacciexe mmIe)etttaoallcaaossmssaapyysls.e.teAAatttohottoaamll ooeffa33n22d,,88w99e88reppeearrssskooenndss tppoaarrrettiticuciripnpaatitteedwd ((ittthaabballee 1n.). 11772266..00000022 _Motes1ses 3M MN01661884 `WashFFioonrrgttthhoiinss Cpporrouojnjeetccytt wwreeesiwwdieilnllltsuussaeendssppweehccoiim,meetnnhsesrfefrfrooomrme,ppaaarrzttiiccniiopptaannittsstwwhehhooanaaalrreeytnnioocttcohort TWheassheinigndtoinviCduoaulsntayrererseifdeernrtesd atnod2wthhoe,"tohuetr-eoffo-rec,ouanrtey"nogtroiunp.theAantaotlyaltoicfc3o5h3ort. s"Tsaahmmepspoloeleefsiscnuowdwuiiilvnlllittdbybue"ealssgseerllaoeeruccettpeerdedgfaeffvoroerrrebaadlssssotoaaoyydassss((atsshmeeepecl"tteoaasubbltli-eenot1l1)')-.9.c7o_4uFFnaiitnffyttdy"y--1gnn9rii8onn9uee.pii.nTnddhAiievvtiioaddtguuaeaallldossifsiinn3tr5tti8hhbeeution oo"offutthth-eeossfee-c55o99uiinnntddyii"vvigiddruuoaaullpss giinnav11e99b77l44oowwdaasssaaamss pffoolelllsloowiwnss::19aa7gg4ee a22n55d--441449yy8ee9a.arrsT-s-he2288a;;gaaeggdeeis44t55r-i-b66u44tiyyoeenaarrss. -l-e22v00e;l;saaggoeed66e55t++eryymeeiaanrress c--h11a11.n.geWWieen wcwioilnlllcaeannnatalslyayztzieeoanalslllooovffertthhtiiissmggerrwooiuutpphiffnoorrisnsedeirruvuimdmufaflllusuo.orroAoecnhheemmiiceaall aledvdietlsiotnoaldestaemrmplieneofch1a2n0gienidnivciodnuaclesntfrraotimonCsLoUvEertLimanedwCiLthUinEinIfdiwviildlubaelss.elAecnted stratified by gender and age. additional sample of 120 individuals stratified by gender and age. from CLUE I and CLUE II will be selected Analyzed Table 1. Analyzed Demegraphic Distribution of Total Number (N = 358) of Serum Samples to be -_ inSSaammdeeFienimdailvevidsuailsbboodtthhvyueeaarrsas((nn=l= $599s/vmr)) MFaelmeasles* Males* NoNemuembeberrooff SSun1bbjee%cts 1974 1989 3z2n2 3z22 27 27 iDinDffifefdrereFenentmitailnedsvividuialscdeaacchhyuyeeaarra((119977l44,, 119s98899)) Females 0<40yes <40 yrs >406-06y0 myrs >60 yrs 2 2 20 20 2 20 20 20 2 2 20 20 MMaalleess<t0 yrs w<04080yyrsms 40-60 yrs >>6600 yyrrss 2 20 20 2 2 20 20 2200 2200 t*hAegteim"iAngeen t11h99i88s99prisoppporrsooavvliiddweeaddsbbweerlliotowtwen(()11997744 ddaataa annddd diistrisbutiionbbiyy aabggee bbtyyggeeinnddeeorrwwanass nnoottKknnoowwnn2at the time this proposal was written) hesAasAmsspsulumemsiiinnzgegottfhhee$cc9ooprrrrreoelvlaaittdiioeonnsbbeesttwseseeennctthhepttowwwooemrmeoaefsasu8ur5r%eemmeoendntetsstoeocbbtitan3ine1ed0d%.iidnni11f99e77e44naacnneddb1e199t88w99eei5sn00h8.8,e, tthweostaimmpelpeersiizoedso.f 59 provides a statistical power of 85% to detect a 10% difference between the two time periods. ArAsesssspuuemcmitniinvgegltythh,eenmmedaetanhnePPsFFtOOanSSdlaelrevdveeldlessviiinnatttihhoenaaggee1g3grrpoouupppf<<o44r00,c,a44c00h--66g0r0oauanpn,ddw>6e600easatreiem2a2t5,e,330102,,0aansndad3m3p6l6eppsop(bb4,,0 in eeareaamsccophhneaacgggtiaeevgeggelrryoog,ruuaoppnu))pdwwsti.hilllelTpphsrtiraoosnvvsdiiaddameerdpaldesestvasatiitiaizstsetitiowcincaialilsppool1ww2leepraroplobflfomfmowororfeorearecatthhhsaagannrtoi99us55ip%c%,awltooepddoeeeswttteieemrccottafaate22710082%%%0 ddstaoiifmfdffeeprtrleeeecnnstcc(ee40 in a2m0%ondgifafgeeregnrcoeubpes.twTeheinstswaompgrleouspizs~(ewg.illmaelsno and women). allow for a statistical power of 78% to detect a 20% difference between two groups (eg., men and women). As.As~aayy`NMMoeratehsawsueursrteemmBeieonantntsaslytical will analyzefor up to serum organofisorochemials Gncluding (PPHFROOPSLSN)C) o/uurEsstihiSnnwMggeShhsMiitggSBhh)ippoarraneenssdassluueyrrvteeiaclllaiuilqqauwutiiiddlvleccarhhnsrruaooslrmynatzaneotogefgrxotarrarpupahpchytyt/eo/ed/el8cleuescrcevrtrreouo,smsppBrroeaarcyygaauttnasaonendfdlgeueemomnreommrcaaahslsesspmossppipeueealcclattstrroi(oiommnnecetsltrureyydriunmg (HPLC/E,SMSMS) and evaluate versus an extracted curve. Because general population serum `ssaammpplleess rraannggee iinn tthhee aarreeaaoofftthhee lloowweerr lliimmiittooff qquuaannttiittaattiioonn ((LLLLOOQQ)) ffoorr PPFFOOSS tthhaatt iiss ccuurrrreennttllyy 11772266..00000033 SW_NoT6s1885 3M MN01661885 fsuosereddPFbbyOySNNo(or3rt1thhpwwpeebss)tt for PFOS (31 ppb) aBBtiiooNaaonrnatalhlyywtteiicscataBll,iossaaanmmapplllyeetssircfraoolmmcatthnhiisbsessttruuedddyyucwweilidlll at Northwest Bioanalytical can be reduced bbnnyyootatabppbepeparranoonxaxialimmlyyazazteeteeddllyyuunn1Ititooillnrddttheheeerr LLoofLfLOOQQ mmaaggnintituuddee.. TThhiiss vvaalliiddaattiioonn eeffffoorrtt iiss ccuunm'eenndtlyy uunnddeerrwwaayy.. 4 RISKSBENEFITS cppRrareeImSvvpiiKaooiuuFFSgsoso/nllrBryytEtthoiihnNneecoEaa11lss99Flss77eIee'4c4TsstsSasambnmnlededon/nioottdroioffinnfortth1a1he99e88spp99errroowwupphhmooeessnbneeaddttnhhbbkeeiiaoossnmmuuadbbarjjrskeekicecegtrtrssnsse,,vvdododlaalauutntnaanittaaenenneefddrroeerdbbdmlleottodooooddpcpaaowwrrnteteiisrcrceeeiipp.ccaaoottleelWlleiieennccttaceeaddnnot `cccTooahnmneccpepeairivivogeepnooofstfoeaadncnosyyullbppeosoctttteaenbnntlctioieaaolltdorifisbsokker maaatesattehshriuuismrtteiimdbmaeehnaeekvxxecacenenpdpotttsffibogoernreeppndoosasasssniiobbcillnieefaobbtrrremeedaaewccdihhteechssonoaofsdcfevocnenotrf.nisdfeWiednhe'teeniacaltialtanihltnityoy.t. iooTnuuhttteeccmopoamrmloeepdssooisisnneed)hhuuhsmmauasbansenstvas..enncTTeohhcitiocssubssrteuruedmddyyeiaaimssouttnhrhgee.dffiihnrrasostvtn-essottencepopcutttpoboaeddteeientoteenarrasmmlsiloinnyceeieaixiftfeepdexoxpswpoeiodstshuiurnardeedivv((eiaadrsssumemaleehsa.aessauulNrtrhoeedd bbyy iiiinnnnfdtfeoiorvrrnmimadaaluttadiilooonnisedwwe)inilhtllliafsbbieeeesvgg.eiinvveeoSnntcactttoouisriitrnneiddcdiaivvlaiimdddauuotaanallsgsw..iSSnleloerrnbaa-eowwpciricllelulspbbeaeenttiaaoessdnssaabalyyyleeygdderaaoxnnuppddossaa,enndwaaillitynyhzzdoeeiuvddtidwwiuinitatdhhlisoov.uiutdtNuaol iiidndeednnitvtiiifdfiiueerassl.. At this point, there is no individual identifiers. Statistical data will be At this point, there is no individual pbberenenesefefinittteaadnnddbyvviigrrrttouuaaullpllysy, no individual isk. without individual no individual risk. ``DcceoopmmaprpPuturtPmoteerertorneftfitceiltltceehstisaiiootinnnnairagrogoaaoialosmnimnossslstttouthunhcanaaktuateatuadrhtreheowlorholrioeiczzcnkeekndedeoaddactwccwihcenheesusesnssnwnewin.oloitAlltlicbiIncen~euoussobssebte,a,taatiaointnnnheddeeddisibnebyrya&kewkwecieeoinenprgpgidoinsnofggafttrtthhhehaeeeeHfuHfeootearrhamlmltsotshharanindodzneldy fohfDoarervspestatuusrdtdiemgennnetentssd,t,ateehmmcaoptpnliflsoioydyaeelenseetossialooolfifctkttyhehedplTTwerrdahagieeinn.niinnnggotCCiennuetesera.annnAddccccotolellslasabebtooorratahtrteoorsressorofefcaaopprdppsrrooavvreeeddausstttuhuddoiirecizssewwdhhooonly have signed a confidentiality pledge. 5. DDIISSCCLPLeOOrsSSoUUnsRRwEEe//rCeCOrOeNcNrSSuEiEtNNedTTin 1974and in 1989. Atth timesofblood donation, : papratritciicipPpaaenrntstsossnsisiggwnneeedrdeaargegecenrneureirataeldlccoionnns1see9nn7t4tffoaonrrdmm,i,naall1ll9oo8ww9iin.nggtAhthteeitihrrbebltlioomooeddssanondfddbdaloatotaadttodobobneeautusioseendd,ffoorr `raraeeppssppeeraraoorrvvcceehhddppbubuyryrptpothhoseeseSesScs..chFhoFoooorlor'le'sesaaCcCcohhomommofifmttthihtteeteseseeeecoocnanamFHupmurammpigaanannsR,Rtiethsehegseeeaccanroornccnsshhseea,aanttttftitfossorrJJmmaannauaunanarddryhy,h,2ii2s0t0o00sr00ymftmfeoeerot~mmitwirnnweggre.y.ere: 6. `C.CpOuOrNpNAFoslFAIeIlDlDlaEnppEdeNeNrrTmssTuoIosnInAAstsLwLswIhiIhTTgonoYYaaa/r/IrepIeNlpNePSedSerUgrmUmeRioRtiAftAttceeNNoddn_Cf1toEi~_hdS.he~anavtveieaalcaicctceyes.sssR0teocoooururdrrsreecfcooorrrdtdsismsmusustsuttdhhyaavtvheeataaclloeenggtiiattiiimnmaattee pHHppeeeurearrsalsploottonhnhsaDaeDlleiaepidndpaedeanrntrmtittmmifufieiseeentrnrtstsstaithagrharneatetakkiieseppapltaletlsidsionnogkerrkeoooepoopftmmtclsolosotnccthfkhkiaedaettdedanarwwrteiehahellleoinontcncykkn.oeeotddRtiweiwnnhcuhoeusresndneens..nTofoTohtrhiniitnsshuwuiwsisseinesnatguagnindidsdsyriritannharrae8aetlwlwcyyioiuunnnssgtgeeaoddiofbnftbytyhhee iannoiafnfnyofoyoorrormanmnlaetacotoioitntoohhtnneertrraththcathheataptamtnnmiievgeimeghmpshtolptbfolboeyheyececeooeonssnsrsoiofidftdemhterhereeeioddsTTscresrnoaeanidnisnneeisiiitdntniiisggvvnCeeCesasneuwutnccaethehryroataosrshdrdatHHatecteeaeaalnloottnfhhfolDtaDasbesetptepmamaiernrntettsmmnetesrenrtprnturat.ae.aillcPPppdeeeerrrwdssiiooootnnddha,a,oallaunntddauussee tkktohheefeeyyo..TTrTrarTaahlihicenneoikinnnkegtgeryyaCCicesieesnknptketeteierpvrp,t,entisnainanommreadehsdisoaifrfanfmfneeddroreenaanneddttsddrrrirooseeoosscmsmsoeefdssfreraadoorrmeminrrtteeahhmmeewofofviaivlelyeeedsds.t..h. aWWIIthnncheammenonnonrdrodeaetatttbtahaheaaaannirrnee33te66ttroopbyybereeeaeautrreusssdoseoefwddftoithothuheuteotssuiitddaeeooff eexxiisstteennccee ooff tthhee TTrraaiinniinngg CCeenntteerr,, wweekknnooww ooff nnoo bbrreeaacchhooff ccoonnffiiddeentnitailailtityy,. for PubT"lTihhcee HrreeesasplpotonhnssRiiebblsleeeappreecrrhss,oonnMsss.aarrSeeaDDnrd.r.raGGeCeo.orrgHgeoefWWf.m.aCCno,ommAsstsstotoc.ckkD,,irDDeicirrteeoccrt,too(rro3of0f1t-thh7eeT9r1T-ar3ia2nin3i0inn)ggaCCnedennDttree.rr Kathy J. Helzlsouer, Director ofthe Specimen Banks, 410.955.9727. for Public Health Research, Ms. Sandm C. Hoffman, Asst. Director, (301-791-3230) Kathy J. Helzlsouer, Director of the Specimen Banks, 410-955-9727. and Dr. 77.. CCOOLLLLAABBOORRAATTIIVVEE AAGGRREEEEMMEENNTTSS:: `and J"ToThhhniisss ppHrrooopppkooissnaalsl iiUssninnvooettrsbbiiitnnydd.iinngg uunnitillaa ssiiggnneedd aaggrreeeemmeenntt iiss eexxeeccuutteedd bbeettwweeeenn tthhee 33MM CCoommppaannyy and Johns Hopkins University. 11772266..00000044 33MM_MMNNO011E66611888856 38.. OOTTHHEERR I[RRBB AAPPPPRROOVVAALLSS NAN/A 9. SINGLE PROJECT ASSURANCE 9. SINGLE PROJECT ASSURANCE NaN/A 1100.. PUBLICATIONS PUBLICATIONS derived IrItosims 33tMhMi'ss siicnnuttedeynn.tt t1To0hcceoo--daaeuuttathihloosrrofiintnfhfooirrsmmawaittliiolonnbewwioittuhhtlJJioonhhenndssiHHnotophpekkipinnrsosjUeUcnntiivrveeersrsesiaittryychrreecssoeenaatrrrccahhcee,rrss dUfUrennorddimeveertdthtihhsfiirsssotamauggdarty-he~iseseommlseetenulntydt yJJfoo.ohrhTnnihtssseHHoodowpepntkakitiinlnessasocUUhfnintinhivgviesearrwsnsidiictlylyrebwwseoeouaourlulcddthlirrnpeeuettradapiioninnstethhtshe.eeifpiggrhohtjettcooptpuruebsbleliiassrhhchiinncffooonrrmtmraaatctiito.onn from this study solely for its ow~ teaching and research pttrpo~cs. 11. PROPRIETARY DATA II. PROPRIETARY DATA CompanPPyroraopnprdiriJtcaot~rhynyisinHnfofooprrkmmiatanitsioonUnnwiwivliellrlbsbieya.~ ddressediinnththeeffoianall aaggreremeeentssiiggnneeddbbeettwweeeenntthheeS3MM Company and Johns Hopkins University. 1122.. BBUUDDThGGeEEbTTudget for theworkcontributionofthe investigatorsfom Johas Hopkias University iiss aatatacchheeTddh..e budget for the work contribution of the investiffa~ors from Johns Hopkins University PPeerrssoonnnneell (OOnn--ccaammppuuss)) HNKKaaaatntmhh-yyeYaHHoeellHzzull.s~aoonuugeerr CoRPP-rroiiinlnneccviieoppsnaatlliPiigrnnaovvtejeoessrtctiitggaattoorr ~~ $$R11,a050t000e000/hr G`GHTlolaootnrar-iliYaaOaZZnoee-ppHCppaumanpgus Persounel Secretary Co-investigator Secretary 252000000 Total On-Campus Personnel $$334T005o,,000ta000l000* $9413858,.,000000000 $93,000 PPeerrssoonnnnSeeallnd((rOOafffHf--occfaafmmmppauunss)) Project coordinator JCCSulaldaanirrdtaahraKKBrHoruulomtmfofppmne~an LDLPaaarbtboooajerrmceartat.coonorraoyygreTTdereicnchahntnoiircciiaann TTJouotdtaaitllhOOBffoff-l-CtCoaanmmppuuss PPeerrssoonnnneell Data manager 5$ 443000000 223550000 5$ 14480,,00000000 $215785,,0.00000000000 $ 27,0O0 (SSMeearriuunmtmenBBaaannnckek,M Mealaiecinnttrtieecmniatmyn,ccreeepCC loasc~tesments) `Cost er Participant Sample (Maintenance, electricity, replacements) Cost Per Participant Sample $$222255.0000xx330000 ssaammpplleess == $ 6677,,550000 OOtthheerr EELxxapbpeoenrnassteeossry supplies (specimen tubes, pipettes) `SLCChaooibmmpopppruiautntteogerrrcySSoussutuppsppplp(liFileieeessdsE(xs,percyimiecne tubes, pipettes) shipping containe rs ) TTSoohtitapallpiOOnttghheecrorsEEtxxspp(eeFnnessdeeEssx, dry ice shipping containers) 5225.00 $122252000500...00000000 150.00 s$ 557155 `T*TooTttoaatllaPPlrrsoojjaeencctteCCstooissmttasste((oJJfoohhtnnismseHHtoooppckkoiimnnpssleUUtnnieivvweeorrsrsikit.tyy)) * Total is an estimate of time to complete work. $188,075 $188,075 11772266..00000055 3M_MNOt661887 3M MN01661887 Travel costs by Johns Hopkins University investigators willbe billed direcdly to IM. All ooTbttyrhhaIeevrrMe.lrreecssoeesaatrrscchbhysstt$uuoddhyynsccooHsstotsp(k(ei..n3g.s..,Uaannnaailvlyyettriiscciaatyll ccinoosvsttessstbbigyyaNNtooorrrstthhwwweielslsttbBBeiibooaialnlnaealdylytdtiiicrceaalcl)t)lywwitiolll 3bbMee p.paaAiiddll by 3M. 11772266..00000066 SW_NoT661588 3M MN01661888