Document ONd74mX8NNbR07mDarqvaBmv

Nadloal Department July 22, 1371 K. P. Wheeler X. B. Koiior - 1760 K. S. Tucker - 1760 W. B. Papageorge B2SX I rtctlvtd a copy of tha Bionomics propo^^ for flab work on tha naw aroclor produota from Or. Kan Macak today. X thought you would Ilka ay racoamandatlona for thla work ao you ean naka whatever plana ara nacaaaary for flnanelng aa aoon aa poaalbla If you agree. In aa affort to gat tha Boat uaaful data for tha laaat onay I would propose that wa do aa followai 1. lava dynanlo aeuta tonicity atudlaa parforuad on thraa (3) compounds on two (2) apaelaa aach (Bluaalll and Channal Catflah) for a total of alx (6) atudlaa -- total coat #3,600.00 2. Kara raalduo acounulatlon atudlaa dona on tha thraa (3) eoapounda at thraa (3) lavela on cha two (2) apaalas uantlonad above. Thaaa atudlaa ahould ba parforuad on bath tha aubjaot eanpounda In watar and In food. Thla would ba la aceordanea with Dr. Naaak'a flrot altaraata auggaatlon. Total coot #18,000.00 for four (4) waaka. Tha atudlaatto datormina bath raaldua acounulatlon and raalduaialaarflaaa after expoeure would probably run at laasl alght (8) waaka. Tha aaaond foar (4) waak parlad and any awbaaquant parlod wanld ooat #2,000.00 par attidy or a total additional coat of #12,000.00 far tha aacond four (4) waaka. Tha total aatlantad coat of tha atudy for eight (8) waoka would bo #33,600.00 ta Btanonles, Inc. Tha analyala of thaaa aanploa to bo dona by honaanta Organic Division Kaaoarch would rapraoant anothor appraalabla lncranant of cost. Tha saaplas would ba shipped by Bionomics ta St. Louis within 48 hours at sampling. Thaaa aanploa must than ba analyxad with ocnaldarabla alaorlty ta ba uaaful to tho study and to avoid unnocaaaary coats (about #600.00/day). An examination of Dr. Naaak'a first altaraata raccanandatlon will show that ho proposed to run one eight-unit MONS 099661 Mr. M. B. Papageorge July 22, 1971 Paga - 2 - ayatan for each compound and each epeelee. Since we hava thraa (3) conpounda and two (2) apaclaa ba coneequently propoaaa to run alx (6) auch eight-unit ayatana. Each eight-unit ayataa will hava two (2) control unlta and alx exparlaantal unlta. Thraa (3) of tha experimental unlta will ba for water-borna toxlcanta at thraa (3) lavela of expoaure and tha remaining thraa (3) for rood-borna toxicant atudlaa at thraa (3) levela of expoaura. If you coablna tha two control aanplaa In aach of tha alx axparlaental eight-unit ayatana, tha total flah aanple load would ba 42 aanplaa at aaoh aanpllng run. Dr. Macak propoaaa tan (10) aanpllng rune if tha axparlnant runa tha full eight (8) waalca. Thaaa aanple runa would bo on tha flrat (1), third (3), aewanth (7), fourteenth l4), twenty-flrat (21), twenty-eighth (28), twenty-ninth i29), thlrty-ftrat (31). thirty-fifth (35), forty-aacond 42, and flfty-alxth (56)day after the axparlnant atarta. Thla rapraaanta tan aanpllng runa with 42 aanplaa par run for a total of 420 aanplaa In alght (8) waaka. Thaaa 420 aanplaa only repreaent tha flab tlaaue aanplaa and not any water aanplaa. It appeara to me that tha Uniting factor In thla propoaal la analytical tlna. It la aaaantlal that we talk to tha analytical reaearch people (Dr'e. Keller and Tuekar) about thla problem and aoa If, In fact, tha whole effort nuat not ba United to our analytleal capability. Thla ahould ba dona aa aoon aa It appeara that nonay la available for thla type of reaearch. Vo ahould give Kan Macak aone kind of anawer aa aoon aa ponalbla. Bear in nlnd that wa can nan one (1) apeele at thraa (3) conoeatratlona on thraa (3) conpounda with tha reaultant analytical requirement of 210 aanplaa In alght (8) waaka, or one (l) apacla at thraa (3) conoantratlana in either tha water or food with tha production of 140 aanplaa. It la alao poaalbla to reduce the aanpllng runa to appreciably reduce tha analytical burden. /bka Jack T. Qarrett MUHS 099661