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AR226-3004 . DuPontHLR2C7-4 HASKELL LABORATORY FOR TOXICOLOGY AND INDUSTRIAL MEDICINE STATIC, ACUTE 96-HOUR SCREENING TEST OF H-20431 TO FATHEAD MINNOWS, P/mphte pfoimlu PURPOSE: This is a rangefinding study designed to provide an estimate of aquatic toxicrtyusing fixed fst concentrations and data measurements at predetermined Intervals. GENERAL INFORMATION, MR: HASKEU. NUMBER: TEST CODE: SPONSOR: NOTEBOOK ID: MATERIAL TESTED: SYNONYMS/OTHER CODES: 295 uPont Specialty < ^ CAS REGISTRY: PURITY: _~'_-9_9._5%_ Page 1 of 2 Company Sanitized. Doss not contain TSCA CBi 9. f HASKELL LABORATORY FOR TOXICOLOGY AND INDUSTRIAL MEDICINE DuPontHLRUT-84 STATIC. ACUTE 96-HOUR SCREENING TEST OF H-20431 TO FATHEAD MINNOWS. PImephulef promla. Continued TEST CONDITIONS Nominal concentration* tested: Dtesolved Oxygen and pH: 0. 0.5.1.0. 50. 500 and 5000 mg/L. Nominal Concentration (mg/L) At test initiation, 0 Hour* Dissolved Oxygen pH (mg/L) 0 0.5 1.0 50 500 5000 8.5 7.4 8.6 7.4 8.5 7.4 8.4 7.3 8.4 7.0 8.3 6.0 "Reac'Tigs taken at 24 hours due to total mortality. "Readings taken at approximately 4 hours due to lota; mortality. At test completion, 96 Hour* Dissolved Oxygen pH (mgrt.) 6.9 7.2 6.5 7.2 6.0 7.2 8.1" 7.1* 8.5" 8.31' 7.16.sf RESULTS Based on visual observations, all solutions were clear except 50. 500 and 5000 mg/L which were cloudy. All solutions had air bubbles collecting on the inside of 'est vessels. Mortalities were 0. 0,0,100,100, and 100%, respectively. All water quality parameters were wrt-.in acceptaole limits. Loading (0.08 g/L) was also within acceptable limits. CONCLUSIONS H-20431 exhibited moderate toxicity in a 96-hour. unaerated, static acute test using fathead minnows, Pimophales promelas. WORK BY: APPROVED BY: ^^ - Jcfrnes W. Wetzel <- ^ Toxicology Associate W -<AJ i-' // /.- (' / / , " / , . ; . ' ./ . ^icheDe R. Brown. B. A. ,' Study Director DISTRIBUTION: K. D. Daslur (1) E.L.Holloway(l) K.G.Rai(ord(1) M. R. Brown (1) ^ . ^ Mo/Day/Year Mo/Day/Year Company Sanitized. Does not contain TSCA CBI