Document OEy1ok2dZoRB8wKBQOG5BbbD1

AR226-3089 DuPont HL-1998-01379 HASKELL LABORATORY FOR TOXICOLOGY AND INDUSTRIAL MEDICINE H-23005: STATIC, ACUTE 48-HOUR SCREENING TEST TO Daphnia magna PURPOSE: This is a rangefinding study designed to provide an estimate of aquatic toxicity using fixed test concentrations and data measurements at predetermined intervals. GENERAL INFORMATION WORK REQUEST NUMBER: vmHASKELL NUMBER: TEST CODE: MATERIAL TESTED: flrSYNONYMS/CODES: 23005 SPONSOR: NOTEBOOK ID: S U B M IT T E R 'S NOTEBOOK NUMBER: COMPOSITION: DuPont Specialty Chemicals None available CONTAMINANTS; CAS REGISTRY: None available PURITY: Not supplied by sponsor. TEST CONDITIONS Nominal concentrations tested: 0,0.5, 1.0, 50, 500, and 5000 mg/L. Nominal Concentration (mg/L) 0 0.5 1.0 50 500 5000 Test Initiation (0 hours) Test Completion (48 hours) Dissolved Dissolved Oxygen Oxygen (mg/L) A* Bf ________ PH________ _______(mg/L)_______ ________ E l ________ A+ Bf A* Bf A* Bf 8.8 8.7 13 13 9.6 9.6 7.5 7.5 8.8 8.6 13 13 9.6 9.5 7.5 7.5 8.6 8.6 13 7.2 9.6 9.5 7.5 7.5 8.5 8.6 6.7 6.6 9.7 9.6 7.5 7.5 8.6 8.6 6.1 6.1 9.7 9.6 7.5 7.5 8.6 8.5 5.2 5.2 8.7a 8.1a 5.5a 5.5a *A and B represent replicate test chambers within a dose level concentration. a Measurement taken on day 1 (24 hours) due to total immobility. Company Sanitized. Does not contain TSCA CSf Page 1 o f2 DuPont HL-1998-01379 H-23005: STATIC, ACUTE 48-HOUR SCREENING TEST TO Daphnia magna, CONTINUED RESULTS - Based on visual observations, the water control solution and the 0.5,1.0,50, and 500 mg/L test solutions all were clear with no color. The 5000 mg/L test solution was cloudy yellow-white. After 24 and 48 hours undesolved test substance was found at the bottom of all test vessels of 500 and 5000 mg/L test solutions. Immobilities were 0 ,0 ,0 , 0,70, and 100% in the 0,0.5,1.0,50,500 and 5000 mg/L, respectively. The 48-hour EC50, based on nominal concentrations and immobility, was between 50 and 500 mg/L. All water quality parameters were within acceptable limits except the pH of 5000 mg/L was low. CONCLUSIONS ' * H-23005 exhibited slight toxicity in a 48-hour, unaerated, static acute test using Daphnia magna. Study Director w/tm Monm/Day/Year m gwr Monm/DawYear Campastf ' imaiitzeeh Does not c o t a i C A Page 2 of 2