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BIODEGRADATION (BOD/COD) TEST SUBSTANCE Identity:A mixturecontainingperfluorooctanesulfonatweh,ich may alsobe referredtoas PFOS, FC-95, or as a component of FC-3041. (1Octanesulfoniaccid)(CAS # 2795-39-3). Remarks: The 3M productionlotnumber was 2300. The testsample isFC3041 (cc809-1).Currentinformatioinndicatesitisa mixtureof6% PFOS, 36% diethyleneglycolbutylether,36.05% water,12% Sultonefoamer,9% sodium octylsulfate0,.55% sodium laurylsulfatea,nd 0.4% tolyltriazole. The followingsummary applies toa mixture with incompletely charactedzed concentrations of impuddes. Data may not accuratelyreflect the biodegradabilityof the tluorachomical component of the testsample. METHOD: Method: BOD ProtocolofEAR (2/29/80). Type: Aerobic GLP: No Year completed: -1981 Contact brrie:20 days lnoculum: Stalesewage, Chemolite,Coffage Grove, MN. RESULTS BiochemicalOxygen Demand (BOD): 5-Day: 12-Day: 20-Day: 164,000 - 329,000 mg/kg 645,000 mg/kg 642,000 mglkg CONCLUSIONS No conclusionsgiven. Submifter: 3M Company, EnvironmentalLaboratory,P.O. Box 33331, St.Paul, Minnesota,55133 DATA QUALITY Reliabilitryd:imischranking3. The study lacksdescriptonand recordsofthe method followed.The inoculumisnotproperlycharacterizedT.here isno characterizatioonfthe testsubstance puritya,nd the studylacksanalytical confirmatioonfthe amount offluorochemicaplroportioninsoluton.The testing was conducted on a mixtureofchemicalshavingvadng degrees of biodegradabilitlye,adingtopotentalmisinterpretatioofnthe results. REFERENCES Thisstudywas conducted by the 3M Company, EnvironmentalLaboratory,St. Paul,MN, Lab Request number 6508S, 1981. OTHER Lastchanged: 6/26/00 ANALYSIS FORM 1038A t4LT,ftlA-rEgoo COiief-AJMAr-zW 0,F 2 3 so rrte_ s 4 5 6 7 @m, a 9 41 10 11 12 13 14 15 16 17 is 19 20 21 22 23 24 25 26 27 211 1^9 30 31 ..32 23 L4 _ 35 rip A 0 E F i x FILE SEC DAT RiEsatr9 c, PR 'co e* A dLe-&4 LAT-4t6 Ar-coR-j)iAIC7?-0 3&7 2,F T-1 WAronnuntMLaborstorV BOD WorkShm Sompla Lab Req.No. Analyst 37@gi 600 Run No 9/- Arwtya -!Sr' -Day BOD Readings t Date hi Sample Dftcription Fe-30ill 4 o r 2 300, InitiaDlilution (ce gc>,?- of Sample dilutedto /00 mi with D Iwater InitiaDlilutionFactor (Dilutiofnactor= 1 ifundiluted) Botdo NO. fo B,g Cone.of Somplal Somple SIZ&2 Initial Pr@nt 0.0. IDI) D.O. ID2) 2.0 11 '1,0 x-80 -14 -8.0 'T,75*- 12 <O.r Ict < TotalD.O. Depletion IDI - C121 @i_lo DepletioDnue To Sompis ;L,80 g-l@ -Day 130D Avorep @5 Day SOD 3:L 4000 D4DO ttt5g -Y"lo B p T E -C, --IILTS 35 2..,8 0 -'E,i;6 6.6.7 4,:-0-.@ 5.5, oc?0 4 T,DEP. D D'3. BUD Note: I Conc. Concentratioonf sample(ml/I009 inSOD bottles. 2. Sample Size ml or g of sample or dilutedsample added to SOD Bottle. Comments A.? 2214 Li E,'OD :fE '3T 5. 4.-7 5 7,1--3 5 E.. U LT ql EDSP e- P-OD #-j,5- d v 8 -0 BUD T E':-:-'RT U L T.-. 8. ri 6. 15 TD-EP' 146"@r- D 1)E@. 210. 55)c@ I Form 19756 PWO-- 6*iran M"l Lobbratory .BOD Work Shut -UMPIN Lob Req. No. Date Stw"d 4. Au'*Nno.0 goo 'FtWings- sernple Dowiption olel ln!tw Dilution InitiaDlilutionFactor tbr 2300 of Semple dilutedto ..1494) mi with Di water Dilutionfactor I ifundiluted) settle Cone.of UMPISI !60 11 &00IV SN"Plo Siz@2 2-.0 lnithd P.O. (DI) Prumt 0-0- (D2) o2 d7'0 TotalaI.0. DWI*Tion DeplefianDue To So,mplo -Day SOD D" SOD BOD TE@'@T Conc. Concentratioonf sample(mlA or mgA) inBOD bottles. 2. Sample Size mi org of sampleor dilutedsample addedto SOD Battle. E;Mments l@4 T'IfE P@:: 0 D-- I..:,Fwm in*Md Dgution Fwtoo lootdo, Coom of -1@ O@ Gs ti@utod 7:r it 40 -3@il 7 m wit und* bay A vwago :Boo. Conc. Concentraotnio@f"sam pis(rnla.omrg A)@ih80 ti 2. Sample Size mi.or'gof sample or dilutedsampleadded to SOD Bottle. 2214 *wm 197M PM ribw"nmenteLl-aboratorv' SOD Wo* Shoot-BWnks, 'Controakr,WSundards LabRequat No. Analyst /,t,), S"d Type a Source Dow Ste 12-13-91 concentration Flunt4o. If .,I.I - AN*IM - Day SOD Readings NonseededDilu6m -Waur Blank lnhw D.O. (Al) 9Q Seeded Dilution-Waur Blank lopftm) Battle NO. lnithdD.O. (CI) go Seed Convois Botdo NO. Somple sit@ prowt 0.0. fA2) i@o Pruent D.O. (C2) InitiaDl.O. lei) D.O. Dq"tlm,' (Al -A2) qo D.O. Depletion (Cl -C2) o- 55 Pr@t D.O. IB2) Av- D.O. Depletion (A, X2) 0, 3E3 Avwop D.O. CWpWflon (El -E2) .61 0.0. D@Wation @60 ILO 1 Gluom -GlutemiAccidStandwd Bottle NO. sampi* size Inioel0.0. Prsmt D.O. ID21 13 12S o 7,0 <1,4 s- -3.s@o SA"4d inytow um Hyd.owo, Pa.ti.slawStffndord (updu.od) odttle NO. Swnpk Size InitiaDl.O. (DI) pl@ t D.O. (D2) "P",pt**13 el TotalD.O. DhW&don (Di -D2) D -45- TotalDa. Depletion (Di - D2@ ols 0,.r kt4444A,@, Oq"tion Due To gtwwwd 6.I-ey 300 pya DepletiontkW 4:. Day Ave op SOD -*'S- -Dy 00 >mg zx -Day INHD-- 2C33) ,Z Form 19755 PWO 6,446 ""TAY ltoi@@tM. Lob43tator k Shiist oft,and StmW6* Lob Request No. 0 Aknelyst Arco 3 v4moonva"n NonindDeidlutWiaotneB.rlank RotN"o*. lnh0w.0. 4A,) Dm Surted BOD'Ru'Nno@. DOY. 800 ReadinP -1 crz. pi 10.0. IA2) D.OD.eoWt"': (Al-A2) C) -1 Li Awwap D.O. Depletion tAl A2) I aQ S"ded Dilution - Water Blank (opdonw) so Prominit 0.0.' (C2) D.O. D%AMI" (Cl C2) o Awwap D.O. Depletion S-"d Controls.. Botoo.w.. Samoa sin od: Initial 0.0. lei) Pr@nt D.O. 1621 0.0. owletion GlucosGelufamAictidStendwd Bot"e No. SempleSize InitiDa.lO. IDI) Pt tD.O. (D2) .7,0 -,A ila@lmydiropi,P@,O,WatgS dl.d(apd..md) 00"10 NO. SONP'* InitialD.O. (Di,) Po@ t D.O. (D 2) -0,9*ro19755 PVWO TotalD.O. D@pWdon IDI -02) DepletioDnue'.. To Standwd Dy so 0 -70 Total DD. Dopkidon (Di D21 Of Flo 30 30 pwa H&08nZ" Deplation4W* soa#W Dy Sol).-- L r7lO A.&P 2f.33) 1(0 -imenimLaborstw 'I@o@t@o .-EindStiit@" .2-2 O,M @Lib'Request No. Date Started Seed Type Source &t:@, (j adnolo of18"d iconcentration 1 0/.0 wateerlm* SOD Run NO. Analyn Day BOD Readings Date &mle No. i.ih 0.0. (AlI p am t0.0. (A2) C3o D.O.Depletion (Al A2) go 'AlviiDr.iOw. Ow"on (Al A21 Seeded Dilution -Waftr Blank, (aption#A) -70. '767 sonm NO. kthw D.O. D.O. Depletion (C-1 C2) 36 Avwap D.O. PW*tion ILI -C2). @7, S"d Confiols Bottl* No@. SN"Ple size ID 60. InMal D.O.. Pramt D.O. (B2) ,:oore-). ------f D.O. Ddko4d*n IBI 82) Glt4cose - Glutemic Acid Stands So .itio@No. son*m .size 0.0. 13 7.0 WtialD.O. No. Swnpiesize.,. sq 19755PWO plow tD.O. 47.70 1 D.O. iotion D2) Total0.0. Dw"an Depletion Due To Stendard q. A3 -BW pay 41a 3 3'4(. -j Dopmtiongo* Day -T*4k@ndwd A--map Dev 40 2.C!3 Dw Z, og