Document OE7OVwYvMqQY63DX0redQX6V1

COVAH^ THE DEVELOPMENT SERVICES COMPANY Sponsor: 3M St. Paul, Minnesota FINAL REPORT Study Title: 104-Week Dietary Chronic Toxicity and Carcinogenicity Study with Perfluorooctane Sulfonic Acid Potassium Salt (PFOS; T-6295) in Rats Author: Peter J. Thomford, PhD Study Completion Date: January 2, 2002 Testing Facility: Covance Laboratories Inc. 3301 Kinsman Boulevard Madison, Wisconsin 53704-2595 Laboratory Study Identification: Covance 6329-183 Sponsor Study Identification: 3M T-6295 Volume I of IX Page 1 of 4068 Covance 6329-183 3M T-6295 COMPLIANCE STATEMENT 104-Week Dietary Chronic Toxicity and Carcinogenicity Study with Perfluorooctane Sulfonic Acid Potassium Salt (PFOS; T-6295) in Rats All aspects of this study were in accordance with the Good Laboratory Practice Regulations as set forth in Title 40 of the US Code of Federal Regulations Part 792, issued November 29, 1983 (effective December 29, 1983), and with any applicable amendments. 2 Covance 6329-183 3M T-6295 QUALITY ASSURANCE STATEMENT This report, with the exception of Appendices 7 and 8, has been reviewed by the Quality Assurance Unit of Covance Laboratories Inc., in accordance with the Environmental Protection Agency (EPA) Good Laboratory Practice Standards, 40 CFR 792 . The following inspections were conducted and findings reported to the study director and study director management. Inspection Dates_______ From To Phase 04/21/98 04/21/98 Protocol Review 05/11/98 05/11/98 Food Consumption 05/14/98 05/14/98 Test Article Preparation 07/20/98 07/20/98 Body Weight 07/20/98 07/20/98 Protocol Amendment Review 10/19/98 10/19/98 Test Article Administration 01/18/99 01/18/99 Animal Observation 04/20/00 04/20/99 Clinical Laboratory Inspection 06/02/99 06/04/99 Protocol Amendment Review 11/08/99 11/08/99 Test Article Administration 02/28/00 02/28/00 Protocol Amendment Review 04/19/00 04/19/00 Postlife 05/02/00 05/22/00 Data Review 09/20/00 10/12/00 Data Review 10/10/00 11/03/00 Report Review 05/15/01 05/17/01 Report Review 12/05/01 12/13/01 Report Review 01/02/02 01/02/02 Protocol Amendment Review 01/02/02 01/02/02 Protocol Amendment Review Date Reported to Study Director and Study Director Management 04/21/98 05/11/98 05/14/98 07/20/98 07/20/98 10/19/98 01/18/99 04/20/99 06/04/99 11/08/99 02/28/00 04/19/00 05/23/00 10/17/00 11/08/00 05/17/01 12/17/01 01/02/02 01/02/02 3 Covance 6329-183 3M T-6295 STUDY IDENTIFICATION 104-Week Dietary Chronic Toxicity and Carcinogenicity Study with Perfluorooctane Sulfonic Acid Potassium Salt (PFOS; T-6295) in Rats Test Material: Perfluorooctane sulfonic acid potassium salt (PFOS; T-6295) Sponsor: 3M Toxicology Services Building 220-2E-02, 3M Center St. Paul, Minnesota 55144-1000 Study Monitor: Andrew M. Seacat, PhD 3M 651.575.3161 Alternate Study Monitor: John Butenhoff, PhD, DABT 3M 651.733.5180 Study Location: Covance Laboratories Inc. 3301 Kinsman Boulevard Madison, Wisconsin 53704-2595 Study Director: Peter J. Thomford, PhD Covance Laboratories Inc. PO Box 7545 Madison, Wisconsin 53707-7545 608.241.7207 Study Timetable: Study Initiation Date: Experimental Start Date: Inlife End Date: Experimental Termination Date: Study Completion Date: April 15, 1998 April 20, 1998 April 24, 2000 December 21, 2001 January 2, 2002 4 Covance 6329-183 3M T-6295 KEY PERSONNEL 104-Week Dietary Chronic Toxicity and Carcinogenicity Study with Perfluorooctane Sulfonic Acid Potassium Salt (PFOS; T-6295) in Rats Study Director: Study Toxicologist: Study Coordinator: Supervisor, Small-Animal Toxicology: Supervisor, Dose Formulation: Supervisor, Chemistry/Tox Support: Associate Director, Laboratory Animal Medicine: Clinical Pathologist: Supervisor, Clinical Pathology: Anatomic Pathologist: Supervisor, Anatomic Pathology: Supervisor, Anatomic Pathology: Staff Scientist, Safety Assessment and Biostatistics: Principal Scientist and Biostatistician, Safety Assessment and Biostatistics: Anatomic Pathologist, Cell Proliferation: Anatomic Pathologist, Electron Microscopy: Peter J. Thomford, PhD Thomas E. Ryan, BS Nii van Bruce-Konuah Pamela D. Gabris, BS, LAT Dixie Bushee, BS, LATG Mark Hoffmann, BS, MBA Donna J. Clemons, DVM, MS Diplomate, ACLAM Robert L. Hall, DVM, PhD Diplomate, ACVP (Clinical Pathology) Ronald Markevitch, BS, MT (ASCP) Richard D. Alsaker, DVM, MS Diplomate, ACVP Diplomate, ABT Kimberly W. Durland, BS, HT Laurie J. Schuller, BA, LAT Arjun Roy, MA Ajit K. Thakur, PhD Sandra R. Eldridge, PhD Pathology Associates International James B. Nold, DVM, PhD Diplomate, ACVP Pathology Associates International 5 Covance 6329-183 3M T-6295 VOLUME I OF IX CONTENTS Page ABSTRACT.......................................................................................................................11 PURPOSE.......................................................................................................................... 17 REGULATORY COMPLIANCE..................................................................................... 17 TEST MATERIAL AND VEHICLE................................................................................ 17 Test Material..................................................................................................................17 Vehicle........................................................................................................................... 17 Carrier............................................................................................................................ 18 Reserve (Archive) Samples............................................................................................18 Disposition.....................................................................................................................18 TEST ANIMALS AND HUSBANDRY........................................................................... 18 Animals.......................................................................................................................... 18 Identification..................................................................................................................18 Justification....................................................................................................................19 Husbandry......................................................................................................................19 Acclimation....................................................................................................................19 PROCEDURES................................................................................................................. 20 Major Computer Systems............................................................................................. 20 Group Designations and Dose Levels...........................................................................20 Dosing Procedures.........................................................................................................21 Dose Analysis................................................................................................................ 23 Observation of Animals................................................................................................ 23 Clinical Pathology.........................................................................................................24 Serum PFOS Analyses...................................................................................................25 Anatomic Pathology......................................................................................................26 Interim Sacrifices..........................................................................................................26 Terminal Sacrifice.........................................................................................................26 Recovery Sacrifice........................................................................................................27 Statistical Evaluation.....................................................................................................30 Survival and Tumor Analysis....................................................................................... 30 Record Retention........................................................................................................... 33 RESULTS AND DISCUSSION....................................................................................... 33 Dose Analysis................................................................................................................ 33 Observation of Animals.................................................................................................34 Clinical Pathology.........................................................................................................36 Anatomic Pathology......................................................................................................37 Cell Proliferation Results.............................................................................................. 39 6 Covance 6329-183 3M T-6295 VOLUME I OF IX CONTENTS (Continued) Page RESULTS AND DISCUSSION (Continued) Electron Microscopy Results........................................................................................ 39 Characterization of Tumor Incidence Outcome............................................................ 40 CONCLUSION................................................................................................................. 41 SIGNATURES.................................................................................................................. 42 REFERENCES.................................................................................................................. 43 PATHOLOGY REPORT.................................................................................................. 44 PATHOLOGY PEER REVIEW STATEMENT.............................................................. 52 STATISTICAL REPORT OF SURVIVAL, NONNEOPLASTIC, AND NEOPLASTIC LESIONS................................................................................. 56 COMMENTS ON THE DATA........................................................................................ 93 CODES, ABBREVIATIONS, AND UNITS.................................................................... 96 General Codes and Abbreviations................................................................................. 97 Codes for Clinical Pathology........................................................................................ 98 Abbreviations and Units for Clinical Hematology.....................................................101 Abbreviations and Units for Clinical Chemistry........................................................ 103 Abbreviations and Units for Clinical Urinalysis......................................................... 105 Abbreviations and Units for Urine Chemistry.............................................................106 Codes for Anatomic Pathology................................................................................... 107 FIGURES 1 Survival Data - Males............................................................................................109 2 Survival Data - Females.........................................................................................110 3 Mean Body Weight Data (g) - Males....................................................................111 4 Mean Body Weight Data (g) - Females............................................................... 112 5 Mean Food Consumption Data (g) - M ales.......................................................... 113 6 Mean Food Consumption Data (g) - Females....................................................... 114 TABLES 1 Results of Homogeneity Analyses (ppm)..............................................................115 2 Results of Stability Analyses (ppm)...................................................................... 119 3 Results of Dose Preparation Analysis (ppm)........................................................ 121 4 Survival Data......................................................................................................... 132 5 Summary of Clinical Observations........................................................................ 150 6 Summary of Mass Observations............................................................................ 170 7 Covance 6329-183 3M T-6295 VOLUME I OF IX CONTENTS (Continued) Page TABLES 7 Summary of Body Weight Data (g)....................................................................... 176 8 Summary of Body Weight Change Data (g)......................................................... 188 9 Summary of Food Consumption Data (g)............................................................. 200 10 Summary of Test Material Consumption Data (mg/kg/day)................................. 212 11 Summary of Clinical Hematology Data - Week 4.................................................218 12 Summary of Clinical Hematology Data - Week 14............................................ 222 13 Summary of Clinical Hematology Data - Week 27............................................ 226 14 Summary of Clinical Hematology Data - Week 53............................................ 230 15 Summary of Clinical Chemistry Data - Week 4 ................................................... 234 16 Summary of Clinical Chemistry Data - Week 14(PCOAO)..................................240 17 Summary of Clinical Chemistry Data - Week 14.................................................. 242 18 Summary of Clinical Chemistry Data - Week 27.................................................. 246 19 Summary of Clinical Chemistry Data - Week 53.................................................. 250 20 Summary of Clinical Chemistry Data - Week 105................................................ 254 21 Summary of Clinical Chemistry Data - Day 719..................................................256 22 Summary of Clinical Urinalysis Data - Week 4....................................................257 23 Summary of Clinical Urinalysis Data - Week 14..................................................259 24 Summary of Clinical Urinalysis Data - Week 27..................................................261 25 Summary of Clinical Urinalysis Data - Week 53..................................................263 26 Summary of Urine Chemistry Data - Week 4 ....................................................... 265 27 Summary of Urine Chemistry Data - Week 14.....................................................267 28 Summary of Urine Chemistry Data - Week 27.....................................................269 29 Summary of Urine Chemistry Data - Week 53.....................................................271 30 Summary of Organ Weight Data - Week 14 Sacrifice....................................... 273 31Summary of Organ Weight Data - Week 53 Sacrifice............................................ 303 32 Incidence of Macroscopic Observations - Week 14 Sacrifice...............................318 33 Incidence of Macroscopic Observations - Week 53 Sacrifice...............................320 34 Incidence of Macroscopic Observations - All Animals........................................ 333 35 Incidence of Macroscopic Observations - Recovery Sacrifice............................. 357 36 Incidence of Microscopic Observations - Week 14 Interim Sacrifice................ 372 37 Incidence of Microscopic Observations - Week 53 Interim Sacrifice................ 385 38 Incidence of Microscopic Observations - Terminal Sacrifice............................... 401 39 Incidence of Microscopic Observations - Unscheduled Deaths and Sacrifices.... 425 40 Incidence of Microscopic Observations - All Animals........................................ 460 41 Incidence of Microscopic Observations - Recovery Sacrifice..............................497 APPENDIX 1.................................................................................................................. 514 Protocol Deviations.....................................................................................................515 8 Covance 6329-183 3M T-6295 VOLUME II OF IX CONTENTS (Continued) Page APPENDIX 2 .................................................................................................................. 520 Individual Animal Fate Data....................................................................................... 521 Individual Clinical Observations................................................................................. 543 VOLUME III OF IX APPENDIX 2 (Continued)..............................................................................................809 Individual Clinical Observations................................................................................. 810 VOLUME IV OF IX APPENDIX 3................................................................................................................ 1257 Individual Body Weight Data (g).............................................................................. 1258 APPENDIX 4 ................................................................................................................ 1345 Individual Food Consumption Data (g).....................................................................1346 Individual Test Material Consumption Data (mg/kg/day)........................................ 1432 APPENDIX 5................................................................................................................ 1512 Individual Clinical Hematology Data........................................................................1513 Individual Clinical Chemistry Data...........................................................................1585 Individual Clinical Urinalysis Data...........................................................................1652 Individual Urine Chemistry Data.............................................................................. 1724 VOLUME V OF IX APPENDIX 6................................................................................................................ 1750 Individual Anatomic Pathology Data........................................................................1751 9 Covance 6329-183 3M T-6295 VOLUME VI OF IX CONTENTS (Continued) Page APPENDIX 6 (Continued)........................................................................................... 2144 Individual Anatomic Pathology Data....................................................................... 2145 VOLUME VII OF IX APPENDIX 6 (Continued)........................................................................................... 2497 Individual Anatomic Pathology Data....................................................................... 2498 VOLUME VIII OF IX APPENDIX 6 (Continued)........................................................................................... 2855 Individual Anatomic Pathology Data....................................................................... 2856 VOLUME IX OF IX APPENDIX 6 (Continued)........................................................................................... 3455 Individual Anatomic Pathology Data....................................................................... 3456 APPENDIX 7................................................................................................................ 4004 Cell Proliferation Report.......................................................................................... 4005 APPENDIX 8................................................................................................................ 4029 Electron Microscopy Report..................................................................................... 4030 10 ABSTRACT Covance 6329-183 3M T-6295 The purpose of this study was to assess the chronic toxicity and carcinogenicity of Perfluorooctane sulfonic acid potassium salt (PFOS; T-6295), also referred to as T-6295, when administered in the diet to rats for at least 104 weeks. Due to excessive mortality, female animals in Group 3 were terminated during Week 103. Male and female Crl:CD(SD)IGS BR rats were assigned to six groups (70 animals/sex/group for Groups 1 and 5; 60 animals/sex/group for Groups 2, 3, and 4; and 40 animals/sex/group for Group 6). Each group received diets containing acetone, the vehicle (Group 1), 0.5 (Group 2), 2 (Group 3), 5 (Group 4), or 20 (Groups 5 and 6) ppm T-6295. Group 6 was placed on recovery after 52 weeks of treatment. Food was provided ad libitum, except when animals were fasted. Water was provided ad libitum. The animals were observed twice daily (a.m. and p.m.) for mortality and moribundity; findings were recorded as they were observed. At least once prior to treatment and weekly thereafter, each animal was removed from its cage and examined; abnormal findings or an indication of normal was recorded. Body weight data were collected weekly through Week 17, once every 4 weeks thereafter, and at Week 105. Food consumption data were collected weekly for the first 16 weeks and once every 4 weeks thereafter. During Weeks 4, 14, 27, and 53, blood and urine were collected for hematology, clinical chemistry, urinalysis, and urine chemistry tests from 10 animals/sex in Groups 1 through 5. Blood was collected for cholesterol and triglyceride determinations from all animals prior to the terminal sacrifice during Week 105 (Week 103 for females in Group 3) and the recovery sacrifice (Group 6) during Week 106. Blood films were also prepared for animals at the terminal and recovery sacrifices. Five animals/sex in Groups 1 through 5 were sacrificed during Week 4; livers were collected and weighed. [The liver samples were collected for PFOS analysis, mitochondrial activity, hepatocellular proliferation rate measurements by proliferation cell nuclear antigen (PCNA), and determination of palmitoyl-CoA oxidase activity. In addition, liver samples were collected for PFOS analysis (Weeks 14, 53, 103, 105, and 106), hepatocellular proliferation rate measurement [by PCNA at Week 14 and by bromodeoxyuridine (BrdU) immunohistochemistry at Week 53] and palmitoyl-CoA oxidase determination (Week 4 and 14).] A section of duodenum was also collected at Week 53 for BrdU immunohistochemistry. During Weeks 14 (Groups 1 through 5) and 53 (Groups 1 and 5), necropsies were performed on five and 10 animals/sex, 11 Covance 6329-183 3M T-6295 respectively. Surviving females in Group 3 were sacrificed and necropsied during Week 103 because of reduced survival. The remaining animals in Groups 1 through 6 were sacrificed and necropsied during Weeks 105 and 106. At necropsy, macroscopic observations were recorded, organ weights were obtained (Weeks 14 and 53), and tissues were placed in fixative as specified by the protocol. At the terminal and recovery sacrifices, sections of the heart and liver were preserved in 2.0% parafomaldehyde/2.5% gluteraldehyde in 0.1M phosphate buffer, processed, and embedded in epoxy resin for possible future electron microscopy from 10 animals/sex in Groups 1, 4, 5, and 6. Animals that died on test or were sacrificed at unscheduled intervals were also necropsied, but organ weights were not recorded. Microscopic examinations were done on selected tissues (adrenals, brain, eyes, kidney, liver, mesenteric lymph node, pancreas, spleen, testes, and ovaries) from the animals necropsied during Week 14 and on all tissues from the animals in Groups 1 and 5 that were necropsied during Weeks 53, 105, and 106. In addition, microscopic examinations were done on the tissues from animals that died or were sacrificed due to poor health. Macroscopic lesions, liver, lung, kidneys, pancreas, thyroid, testes, and mammary gland (females), and urinary bladder were examined for animals in Groups 2, 3, 4, and 6 that were sacrificed during Weeks 103, 105, and 106. Liver sections from five animals/sex/group from Groups 1 and 5 were stained with Oil Red "O" and examined microscopically. In addition, liver samples from five animals/sex/group were processed and evaluated by electron microscopy. Survival after 104 weeks of treatment was 22.0, 22.0, 34.0, 50.0, 45.0, and 27.0% for males in Groups 1, 2, 3, 4, 5, and 6, respectively, and 50.0, 30.0, 20.0 (after 102 weeks), 34.0, 52.0, and 48.0% (after 104 weeks) for females in Groups 1, 2, 3, 4, 5, and 6, respectively. There was a significant increased trend in survival that occurred in the males that was due to significant increases in survival in mid-high (5.0 ppm) and high-dose (20.0 ppm) groups as compared to that of the control group. None of the other treated groups in the males revealed any significant differences in survival. No significant trend was noted in survival in females. There was a significant decrease in survival in the mid-dose (2.0 ppm) group and not in the mid-high (5.0 ppm) and high-dose (20.0 ppm) groups as compared to that of the control. 12 Covance 6329-183 3M T-6295 There were no clinical observations attributed to administration of the test material. Males given 20 ppm (Groups 5 and 6) had significantly lower mean body weights compared to animals in the control group during Weeks 9 through 37. During recovery the mean body weights for Group 6 males were similar to mean body weights for Group 5 males. Females in Group 5 given 20 ppm had significantly lower body weights compared to animals in the control group during Weeks 3 through 101. Mean body weights for females in Group 6 given 20 ppm were statistically lower than mean body weights for control females during Weeks 3 through 61, but their mean body weights became nearer to the weights of the control females when placed on recovery. At Week 105, mean body weights for surviving males were 98, 108, 103, 101, and 96% of controls for the low- (0.5 ppm), mid- (2.0 ppm), and high-dose groups (20.0 ppm), respectively. At Week 105, mean body weights for surviving females were 101, 107 (Week 101), 100, 86, and 105% of controls, for the low- (0.5 ppm), mid- (2.0 ppm), and high-dose groups (20.0 ppm), respectively. Mean body weight change was statistically significantly lower for males given 20 ppm diets for Weeks 1 through 15 and Weeks 15 through 29. Mean body weight change was statistically significantly lower for females given 20 ppm for Weeks 1 through 15 and Weeks 29 through 57 (Group 5 only). Mean body weight changes for males and females in Group 6 were significantly increased during the several weeks of recovery. Animals at lower dose levels occasionally had statistically significantly decreases in body weight gain; however, these occurrences were inconsistent over time between sexes and were not clearly dose-related. Although not always statistically significant, males given 20 ppm tended to consume less food during Weeks 1 through 24. Food consumption was similar for males given 20 ppm compared to animals given the control material during Weeks 28 through 104. Statistically significantly lower food consumption was noted frequently for females given 20 ppm during Weeks 2 through 44. Food consumption for males and females was similar in all the other treated groups compared to animals given the control material. Dietary administration of PFOS for approximately 53 weeks was associated with mildly higher urea nitrogen for males and females fed 5 or 20 ppm; mildly lower glucose for males and females fed 20 ppm; mildly to moderately lower cholesterol for males and females fed 20 ppm; and mildly higher alanine aminotransferase for males fed 20 ppm. 13 Covance 6329-183 3M T-6295 None of these effects were considered adverse. The effect on cholesterol was no longer apparent after 104 weeks of treatment. There was no effect on hepatic palmitoyl-CoA oxidase activity. Terminal body weights in males and females at Week 14 were comparable between control and treated groups. At the Week 14 interim sacrifice, absolute and relative liver weights were significantly increased in the males given 20 ppm. In females given 20 ppm, only the liver-to-body weight percentage was significantly increased. Test material-related histomorphologic changes were limited to the liver in the males given 5 or 20 ppm and in the females given 20 ppm. The changes consisted of hypertrophy of hepatocytes in centrilobular areas in males and females, and midzonal to centrilobular hepatocytic vacuolation. The incidence and severity of the changes tended to be greater in the males. At the Week 53 interim sacrifice, terminal body weights were significantly decreased in the females given 20 ppm. In the males, absolute and relative liver weights were increased in the group receiving 20 ppm. In addition, absolute and relative spleen weights were decreased in males given 20 ppm. There were no clear or consistent gross observations at the Week 53 interim sacrifice that could be attributed to the administration of the test material. At the Week 53 interim sacrifice, centrilobular hepatocytic hypertrophy and vacuolation was increased in incidence and severity in the males given 20 ppm. Generally, in the females given 20 ppm, only centrilobular hypertrophy was seen, and the change was less severe than that noted in the males. In addition, minimal to slight centrilobular hepatocytic pigment was found in the females given 20 ppm. There were no other histomorphologic changes that could be associated with the administration of the test material. Liver findings in several unscheduled deaths given 20 ppm resembled those seen in animals sacrificed at Week 53. At the terminal sacrifice, the livers of animals given 5 or 20 ppm exhibited a slight increase in macroscopic findings, including enlarged, mottled, diffuse darkened, or focally lightened. Hepatotoxicity, characterized by centrilobular hypertrophy, centrilobular eosinophilic hepatocytic granules, centrilobular hepatocytic pigment, or centrilobular hepatocytic vacuolation was noted in the animals given 5 or 20 ppm. An increase in eosinophilic clear cell altered foci and cystic hepatocellular degeneration was noted in the males given 2, 5, or 20 ppm. 14 Covance 6329-183 3M T-6295 The data suggests that the hepatotoxicity did not persist in the recovery-sacrifice animals. In the unscheduled sacrifices between Weeks 54 and 105, animals given 20 ppm had increased hepatocellular centrilobular hypertrophy, eosinophilic hepatocytic granules, and centrilobular hepatocytic pigment were noted. Increased hepatocellular centrilobular hypertrophy was seen in animals given 5 ppm. A significant positive trend was noted in the incidences of liver hepatocellular adenoma for males. This was associated with a significant increase in the high-dose (20.0 ppm) group over the control. The high-dose recovery (20.0 ppm) group animals in this case exhibited a significantly decreased incidence when compared to that of the high-dose (20.0 ppm) group. Significantly increased incidences were observed for thyroid follicular cell adenoma in the high-dose recovery (20.0 ppm) group when compared to either the control or the high-dose (20.0 ppm) group. There was also a significant increase in the combined (adenoma and carcinoma) thyroid follicular cell tumors in the high-dose recovery (20.0 ppm) group animals as compared to that of the high-dose (20.0 ppm) group. None of these cases were associated with any significant trend. Thyroid follicular cell carcinoma did not show any significant effect. There were no other significant neoplastic findings in the males. Significant positive trends were observed in the incidences of hepatocellular adenoma and hepatocellular combined adenoma and carcinoma in the females. These cases were associated with significant increases in the high-dose (20.0 ppm) group as compared to the control. All these tumors were incidental and noted at the terminal sacrifice. A marginally significant increase for thyroid follicular cell adenoma and carcinoma combined was observed in the mid-high (5.0 ppm) group but not in the high-dose (20.0 ppm) group as compared to the control group. In fact, thyroid C-cell adenoma and thyroid C-cell combined adenoma and carcinoma actually showed significant decreases in the high-dose (20.0 ppm) group as compared to the control. There were significant negative trends in the incidences of mammary fibroadenoma and mammary combined adenoma and fibroadenoma. These cases were associated with significantly lower incidences in the respective high-dose (20.0 ppm) group when compared to that of the control. However, the low-dose (0.5 ppm) group animals for these cases exhibited significant increases over the control. A significant decreased incidence was found for the high-dose recovery (20.0 ppm) group in the incidences of mammary carcinoma as compared to the high-dose (20.0 ppm) group. There was no other significant group effect 15 Covance 6329-183 3M T-6295 in mammary carcinoma. There was a marginal significant negative trend for the mammary combined fibroadenoma/adenoma/carcinoma. However, the low- (0.5 ppm) and mid-high (5.0 ppm) groups for this case exhibited significantly increased incidences over the control. There were no other significant neoplastic findings in the females. There were no statistically significant increases in cell proliferation as measured by Proliferative cell nuclear antigen (PCNA) immunohistochemistry at weeks 4 and 14, or by bromodeoxyuridine (BrdU) immunohistochemistry at week 53. Liver samples were obtained from each of the 20 rats during necropsy at terminal sacrifice for examination by transmission electron microscopy (TEM). Liver samples were assessed for ultrastructural changes that may be related to chronic toxicity of the test material when administered in the diet. Electron microscopic evaluation identified mild to moderate changes related to treatment. Mild to moderate smooth endoplasmic reticulum hyperplasia and minimal to mild hepatocellular hypertrophy were the prominent features found to be different between the high dose group and the control animals. Other changes included a slight increase in the amount of glycogen in treated animals compared with controls. The chronic toxicity and carcinogenicity of the test material, Perfluorooctane sulfonic acid potassium salt (PFOS; T-6295) was assessed following dietary admixture to male and female Crl:CD(SD)IGS BR rats at dose levels of 0.5, 2, 5, or 20 ppm. Based on anatomic pathology findings in the liver, the no-observable-adverse-effect level for Perfluorooctane sulfonic acid potassium salt (PFOS; T-6295) is considered to be 2 ppm. 16 PURPOSE Covance 6329-183 3M T-6295 The purpose of this study was to assess the chronic toxicity and carcinogenicity of Perfluorooctane sulfonic acid potassium salt (PFOS; T-6295), when administered in the diet to rats for at least 104 weeks. Due to excessive mortality, female animals in Group 3 were terminated during Week 103. REGULATORY COMPLIANCE All aspects of this study were in accordance with the Good Laboratory Practice Regulations as set forth in Title 40 of the US Code of Federal Regulations Part 792, issued November 29, 1983 (effective December 29, 1983), and with any applicable amendments. TEST MATERIAL AND VEHICLE Test Material The test material, Perfluorooctane sulfonic acid potassium salt (PFOS; T-6295), Lot No. 217, is a light colored, free flowing powder and is 86.9% pure. It was received at Covance on June 4, 1996, and September 4, 1997. The test material was stored at room temperature. Information on synthesis methods, stability, composition, or other characteristics that define the test material was provided by the sponsor and is on file with Covance. Vehicle The vehicle, acetone (Spectrum Chemical Mfg. Corp., New Brunswick, New Jersey and Gardena, California), Lot Nos. LH0253 and NS0231 (expiration date: June and September 1, 2000, respectively). The shipments were received at Covance on June 23, 1997, and September 8, 1998, respectively. The vehicle was stored at room temperature. 17 Covance 6329-183 3M T-6295 Information on synthesis methods, purity, composition, or other characteristics that define the acetone is on file with the manufacturer. Carrier Certified rodent diet (#5002 meal, PMI Nutrition International) was used as supplied. The lot numbers are recorded in the data. The diet is routinely analyzed by the manufacturer for nutritional components and environmental contaminants. Reserve (Archive) Samples A reserve sample (5 g) of the test material was taken and stored at room temperature. This sample was transferred to the sponsor on June 12, 2000. Disposition Remaining test material was returned to the sponsor on June 12, 2000, after authorization from the sponsor. TEST ANIMALS AND HUSBANDRY Animals Male and female Crl:CD(SD)IGS BR rats were obtained from the Raleigh, North Carolina, facility of Charles River Laboratories on April 7, 1998. At initiation of treatment, the animals were approximately 41 days old; the males weighed from 135 to 226 g, and the females weighed from 128 to 182 g. Identification Each animal was assigned a temporary number upon arrival. Before initiation of treatment, a microchip identification device was implanted into each animal. After randomization for placement on test, each animal was assigned a permanent number, and the microchip was coded with the permanent number. All data for an animal are recorded under these numbers. 18 Covance 6329-183 3M T-6295 Justification Historically, rats have been used in safety evaluation studies and are recommended by appropriate regulatory agencies. Husbandry Animal were housed in Animal rooms 316, 332, or 345 for 13 days during acclimation and then were moved to rooms 3004 or 3005 for the duration of the study. Environmental controls for the animal rooms were set to maintain 18 to 26oC, a relative humidity of 30 to 70%, and a 12-hour light/12-hour dark cycle. The animals were housed individually (except for the first 6 days of acclimation when animals were group-housed) in stainless-steel cages (see Protocol Deviations for exceptions). Some animals were placed in polycarbonate cages during the study when health problems dictated. Certified rodent diet (#5002 meal, PMI Nutrition International) was provided ad libitum during acclimation (see Protocol Deviations for exceptions). Water was provided ad libitum (see Protocol Deviations for exceptions). Samples of the water are analyzed for specified microorganisms and environmental contaminants. The results are on file with Covance-Madison. There were no known contaminants in the diet or water at levels that would have interfered with this study. Animals not selected for the study (42 males and 40 females) were sacrificed and discarded. Acclimation Four hundred and two males and 400 females were received on April 7, 1998, and acclimated in Animal Rooms 316, 332, or 345 for 13 days then moved to rooms 3004 and 3005 before initiation of treatment. In general, animals in this shipment appeared 19 Covance 6329-183 3M T-6295 healthy. During acclimation, the animals were examined for abnormalities indicative of health problems, and body weights were recorded for all animals at randomization. PROCEDURES This study was conducted in accordance with the Protocol dated April 15, 1998, and with Protocol Amendment Nos. 1, 2, 3, 4, and 5. Protocol deviations are in Appendix 1. Major Computer Systems The Path/Tox application, supplied by Xybion Medical Systems Corporation, was used for the direct on-line capture of in-life toxicology and anatomic pathology data. The Randomization and Data Extension Systems (RADES) and Automatic Form and Label Generation System (AFLGS) applications were used in conjunction with the Path/Tox system to randomize animals and produce labels and forms, respectively. The Talisman application was used for the dose preparation information, and the Millennium system was used for the collection of dose analysis data, and the Clinical application was used for the collection of clinical pathology data. The Report Generation System application was used to pull the information from the Path/Tox and clinical applications into Word for reporting purposes. All version numbers of the applications can be found in the log book for the application. Group Designations and Dose Levels The animals were examined by a laboratory animal veterinarian on April 14, 1998, and were found to be suitable for study consideration. Selection of animals for the study was based on acclimation data. Animals were assigned to treatment groups using a computerized blocking procedure designed to achieve body weight balance with respect to treatment group. At the time of randomization, the weight variation of the animals did not exceed +2 standard deviations of the mean body weight for each sex. Group mean body weights were analyzed using Levene's test for homogeneity of variance at the 5.0% probability level and were found to be homogeneous. Animals were assigned to the study according to the following design. 20 Covance 6329-183 3M T-6295 Number of Animals Dietary Levels Group Male Female (ppm T-6295) 1 (Control)^b'c 70 70 0 2 (Low)b 60 60 0.5 3 (Mid)b 60 60 2.0 4 (Mid-High)b 60 60 5.0 5 (High)b,c 70 70 20.0 6 (High Recovery)d 40 40 20.0 a The control animals received the control diet (basal diet with acetone). b Five animals/sex in Groups 1 through 5 were sacrificed at Weeks 4 and 14 for hepatocellular proliferation rate measurements, biochemical analyses (palmitoyl-CoA oxidation), and histopathology (Week 14 only). c Ten animals/sex in Groups 1 and 5 were designated as interim sacrifice animals. These animals were sacrificed after at least 52 weeks of treatment. d Animals in Group 6 were treated for at least 52 weeks, then treatment was discontinued, and the animals were observed for reversibility, persistence, or delayed occurrence of toxic effects for at least 52 weeks posttreatment. During recovery, the animals received basal diet only. Dosing Procedures Dose Preparation. Dietary concentrations were based on the test material as supplied. Before initiation of treatment, diets of 0.5, 1, 2, and 20 ppm concentrations were prepared for stability and homogeneity analyses. Diets were prepared at least once every 4 weeks during the treatment phase of the study. Preparation of Diet for Group 1. The appropriate amount of certified diet was weighed and mixed with an appropriate volume of acetone. Preparation of Base Diet. A specified amount of certified diet was weighed into a labeled container. The required amount of test material was weighed and transferred into a labeled container and the appropriate volume of acetone was added to the container and mixed manually. Additional volume of acetone was added as necessary until the test material dissolved. To prepare a premix, the certified diet was transferred into a labeled Hobart mixing bowl. The test material and acetone were added to the mixing bowl, overlaid with a portion of diet from the mixing bowl, and thoroughly mixed. A portion of 21 Covance 6329-183 3M T-6295 diet from the mixing bowl was transferred to a second mixing bowl, mixed manually to recover residual test material, and returned to the first mixing bowl. The contents of the mixing bowl were thoroughly mixed. Preparation of Diets for Groups 2 through 4. Each dose level was prepared independently. A specified amount of certified diet was weighed into a labeled container and a specified amount of base diet was weighed for each dietary concentration. A pocket was formed in the feed in the mixing bowl and the amount of base diet was transferred to the pocket. The contents of the mixing bowl were thoroughly mixed for 15 minutes. Preparation of Diets for Groups 5 and 6. A specified amount of certified rodent diet (#5002) was weighed into a labeled Hobart mixing bowl and a specified amount of base diet was weighed for each diet. A pocket was formed in the feed in the mixing bowl and the amount of base diet was transferred to the pocket. The contents of the mixing bowl were thoroughly mixed for 15 minutes. Samples for dose analyses were taken directly from the mixing bowl. The diets were stored at room temperature in covered containers until dispensed into feeding jars. Method of Administration. Dietary admixture was used because the potential route of exposure in humans is oral. The dose preparations were administered ad libitum (see Protocol Deviations for exceptions). Animals in Groups 1, 2, 3 (males), 4, and 5 received the test diet for at least 104 weeks. Animals in Group 3 (females) received the test diet for at least 103 weeks. Animals in Group 6 received the test diet for 52 weeks. Retention Samples. Samples (approximately 100 g) were taken from each dose preparation during the inlife phase and stored at room temperature. These samples were discarded on June 15, 2000. 22 Covance 6329-183 3M T-6295 Dose Analysis Analyses for the concentration of test material in the dose preparations were done by Covance using an analytical method, MP-M383-MA, supplied by the sponsor and validated by Covance. Homogeneity was determined from the dose preparations mixed pretest and from preparations mixed for Week 1. One sample (approximately 100 g each) from the top, middle, and bottom of the dose preparations were collected, divided into three subsamples for extraction and analysis, and analyzed for test material content. All samples were stored at room temperature until analyzed within 7 days of mixing. To evaluate the stability of the test material in the carrier, one set of samples (approximately 100 g each) were taken from the preparations mixed pretest, stored at room temperature for 28 days, then analyzed. Homogeneity samples collected from the middle of the pretest dose preparations were analyzed within 2 days of mixing and used as the baseline value. An additional set of samples (approximately 100 g each) were taken from the preparations mixed for Week 1, stored at room temperature for 33 days, then analyzed. Homogeneity samples collected from the middle of the dose preparations for Week 1 were analyzed on the day of mixing and used as the baseline value. For dose confirmation, samples (approximately 100 g each) from all dose preparations were analyzed in duplicate. Homogeneity samples collected from the middle of the dose preparations for Week 1 were used for dose confirmation results. All samples were stored at room temperature until analyzed. Observation of Animals Clinical Observations. The animals were observed twice daily (a.m. and p.m.) for mortality and moribundity. Once prior to treatment and weekly thereafter, each animal was removed from its cage and examined; abnormal findings or an indication of normal was recorded. In addition, the time of onset, location, size, appearance, and progression of each grossly visible or palpable mass were recorded. Body Weights. Individual body weight data were recorded at the time of randomization, weekly for Weeks 1 through 17, once every 4 weeks thereafter, and at Week 105. 23 Covance 6329-183 3M T-6295 Food Consumption. Individual food consumption data were recorded weekly for Weeks 1 through 16 and once every 4 weeks thereafter. Administration of Bromodeoxyuridine. Seven days before the Week 53 interim sacrifice, osmotic pumps (ALZET Model 2ML1) were surgically implanted in five animals/sex/group from Groups 1 and 5. The osmotic pumps were preloaded with approximately 2 mL of bromodeoxyuridine (BrdU) at a concentration of 20 mg/mL. The animals were anesthetized by administration of acepromazine-ketamine-xylazine, and one pump/animal was aseptically inserted subcutaneously (dorsal surface). The incision was closed with wound clips, and the animals were monitored during clinical observations until the time of sacrifice to ensure that there were no clinical signs of infection. One Group 5 male implanted with the osmotic pump was sacrificed before the Week 53 sacrifice. Clinical Pathology Blood and urine samples were collected from 10 animals/sex/group in Groups 1 through 5 during Weeks 4, 14, 27, and 53. Blood films were made and held for possible future examination from animals sacrificed at unscheduled intervals and from animals at scheduled sacrifices after at least 52, 103 (Group 3 females), and 104 weeks of treatment. In addition, blood was collected for cholesterol and triglycerides from all remaining animals at the terminal and recovery sacrifices. Animals were fasted overnight, and urine was collected chilled overnight (approximately 16 hours) before blood sampling. Blood was collected from a jugular vein. The anticoagulant was potassium EDTA for hematology tests. The samples for clinical chemistry samples were collected without anticoagulant. Animals were bled in random order. The following were evaluated. Hematology red blood cell (erythrocyte) count white blood cell (leukocyte) count hemoglobin differential blood cell count hematocrit segmented neutrophil count mean corpuscular volume lymphocyte count mean corpuscular hemoglobin monocyte count mean corpuscular hemoglobin eosinophil count concentration basophil count platelet count blood cell morphology 24 Covance 6329-183 3M T-6295 Reticulocyte smears were made and held for possible future examination. glucose urea nitrogen creatinine total protein albumin globulin cholesterol total bilirubin appearance volume specific gravity pH protein urobilinogen sodium potassium Clinical Chemistry alanine aminotransferase gamma glutamyltransferase aspartate aminotransferase calcium inorganic phosphorus sodium potassium chloride Urinalysis glucose ketones bilirubin blood microscopic examination of Urine Chemistry 16 hour excretion of: sodium potassium Serum PFOS Analyses During Weeks 4 and 14, blood samples (approximately 2 mL) were collected for serum analyses from five animals/sex/group in Groups 1 through 5 (animals selected for hepatocellular proliferation and biochemical analyses). During Week 53, blood samples (approximately 2 mL) were collected from five animals/sex/group in Groups 1 and 5 (from animals selected for interim sacrifice) and from all remaining animals at terminal and recovery sacrifices. Animals were fasted overnight. Blood was collected from a jugular vein, allowed to clot at room temperature, and centrifuged. Samples were collected without anticoagulant. Serum was harvested and stored in a freezer, set to maintain -60 to -80oC, until sent to the sponsor for analyses of PFOS levels and metabolites. Analysis of these samples is the responsibility of the sponsor; results are not included in this report. 25 Covance 6329-183 3M T-6295 Anatomic Pathology Necropsy at Unscheduled Intervals. A necropsy was done on each animal that died or was sacrificed at an unscheduled interval. Animals sacrificed at unscheduled intervals were anesthetized with carbon dioxide, weighed, and exsanguinated (see Protocol Deviations for exceptions). A blood film was taken as part of the necropsy procedure for sacrificed animals. Interim Sacrifices During Weeks 4, five animals/sex/group/interval in Groups 1 through 5 were fasted overnight, bled for serum chemistry, anesthetized with carbon dioxide, weighed, and exsanguinated. The abdominal cavity of each animal was opened, the liver was removed and weighed. Liver samples were collected for palmitoyl-CoA, cell proliferation, and PFOS concentration analyses and for mitochondrial activity. Animals sacrificed during Week 4 were discarded after liver collection. During Week 14, five animals/sex/group/interval in Groups 1 through 5 were fasted overnight, bled for serum chemistry, anesthetized with carbon dioxide, weighed, and exsanguinated. Liver samples were collected for palmitoyl-CoA, cell proliferation, PFOS concentration analyses, and for histopathology. During Week 53, 10 animals/sex/group from Groups 1 and 5 were fasted overnight, bled for serum samples (five animals/sex/group), anesthetized with carbon dioxide, weighed, exsanguinated, and necropsied. The liver was weighed and liver samples were collected and frozen for analysis. Animals sacrificed during Weeks 14 and 53 had macroscopic observations recorded, selected organs weighed, and selected tissues collected and preserved. A blood film was taken for each animal sacrificed at the Week 53 interim sacrifice. Animals were necropsied in random order. Terminal Sacrifice After at least 104 weeks of treatment (Week 103 for females in Group 3), the remaining animals were fasted overnight, bled for serum samples (five animals/sex/group), anesthetized with carbon dioxide, weighed, exsanguinated, and necropsied. Animals were necropsied in random order. 26 Covance 6329-183 3M T-6295 Recovery Sacrifice After at least 52 weeks of treatment and 52 weeks without treatment, the remaining animals in Group 6 were fasted overnight, anesthetized with carbon dioxide, weighed, exsanguinated, and necropsied. A blood film was taken as part of the necropsy procedures at the terminal and recovery sacrifices. The necropsy included a macroscopic examination of the external features of the carcass; all external body orifices; the abdominal, thoracic, and cranial cavities; organs; and tissues. Cell Proliferation Tissue Collection and Immunohistochemical Evaluation. During the Week 4 and 14 tissue collection from animals in Groups 1 through 5 (five animals/sex/group), representative samples of the left lateral lobe and any macroscopic lesions of the liver were collected and preserved in zinc formalin. Liver samples were fixed and embedded in paraffin and blocks were sent to Pathology Associates International (PAI), PAI evaluated the samples (left lateral lobe only) for proliferation cell nuclear antigen (PCNA). In addition, liver sections were stained with hematoxylin and eosin and examined microscopically by PAI. The cell proliferation report is in Appendix 7. Palmitoyl-CoA (PCoA) Oxidase Analyses. During the Week 4 and 14 tissue collections from animals in Groups 1 through 5 (five animals/sex/group), a sample (approximately 500 mg) of the right lateral lobe of the liver was collected and flash-frozen in liquid nitrogen. The samples were stored in a freezer set to maintain -60 to -80oC, until analyzed by Covance for PCoA oxidase activity. Organ Weights. At the interim sacrifice during Weeks 14 and 53, the following organs (when present) were weighed; paired organs were weighed separately. adrenal (2) brain kidney (2) liver lung ovary (2) spleen testes thyroid (2) with parathyroid uterus with cervix 27 Covance 6329-183 3M T-6295 Organ-to-body weight percentages and organ-to-brain weight ratios were calculated. Liver PFOS Analysis. A portion of the liver was collected from five animals/sex/group from Groups 1 through 5 at the Week 4 and 14 interim sacrifices, from five animals/sex/group from Groups 1 and 5 at the Week 53 interim sacrifice, and from all remaining animals at the terminal and recovery sacrifice and flash-frozen in liquid nitrogen and stored in a freezer set to maintain -60 to -80oC, until packed on dry ice and shipped to the sponsor for PFOS and metabolites analyses. Analysis of these samples is the responsibility of the sponsor; results are not included in this report. Mitochondrial Analyses. During the Week 4 tissue collections for animals in Groups 1 through 5, liver samples (approximately 1.5 g) were collected and analyzed by the sponsor for mitochondrial activity. Analysis of these samples is the responsibility of the sponsor; results are not included in this report. Tissue Preservation. The following tissues were collected and preserved in 10% neutral-buffered formalin (see Protocol Deviations for exceptions). adrenal (2) brain cecum cervix colon duodenum epididymis (2) esophagus eye (2) femur with bone marrow (articular surface of the distal end) Harderian gland heart ileum jejunum kidney (2) lesions liver lung with mainstem bronchi lymph node (mesenteric) mammary gland (females only) ovary (2) pancreas pituitary prostate rectum salivary gland [mandibular (2)] sciatic nerve seminal vesicle (2) skeletal muscle (thigh) skin spinal cord (cervical, thoracic, and lumbar) spleen sternum with bone marrow stomach testis (2) thymus thyroid (2) with parathyroid trachea urinary bladder uterus vagina 28 Covance 6329-183 3M T-6295 Bone marrow smears from the femur of each animal at scheduled sacrifices were prepared, stained with Wright's stain, and retained for possible examination. In addition, at the terminal sacrifice, sections of the heart and liver were collected from 10 animals/sex in Groups 1, 4, 5, and 6 and preserved in 2.0% paraformaldehyde/2.5% glutaraldehyde in 0.1M phosphate buffer (see Protocol Deviations for exceptions). The tissues were processed and embedded in epoxy blocks for possible future examination by electron microscopy. Histopathology. Tissues (as appropriate) from each animal in Groups 1, 5, and 6 sacrificed at the Week 53 interim sacrifice were embedded in paraffin, sectioned, stained with hematoxylin and eosin, and examined microscopically. Adrenals, brain, eyes, kidneys, liver, mesenteric lymph node, pancreas, spleen, testes, and ovaries from the animals necropsied at the Week 14 interim sacrifice were also embedded in paraffin, sectioned, stained with hematoxylin and eosin, and examined microscopically. Tissues (as appropriate) from each animal in Groups 1 and 5 sacrificed at the terminal sacrifice and any animals that died on test or were sacrificed at unscheduled intervals were embedded in paraffin, sectioned, stained with hematoxylin and eosin, and examined microscopically. Lesions, liver, lungs, kidneys, pancreas, thyroid, testes, mammary glands (females), and urinary bladder from each animal in Groups 2, 3, 4, and 6 sacrificed at the terminal and recovery sacrifices were embedded in paraffin, sectioned, stained with hematoxylin and eosin, and examined microscopically (see Protocol Deviations for exceptions). Parathyroids were processed with the thyroids, but were not examined. At the Week 53 sacrifice, BrdU immunohistochemistry was performed by PAI on the livers and duodenums from five animals/sex/group in Groups 1 and 5 that received BrdU. In addition, sections of the liver and duodenums from these animals were stained with hematoxylin and eosin and examined microscopically. A pathology peer review was conducted by a pathologist designated by the sponsor. 29 Covance 6329-183 3M T-6295 Liver sections from five animals/sex/group from Groups 1 and 5 were stained with Oil Red "O" stain and examined microscopically. Liver sections in epoxy blocks for five animals/sex/group from Groups 1 and 5 were processed and evaluated by electron microscopy by Pathology Associates International (report included in Appendix 8). Slides of the pancreas from males found dead, sacrificed at an unscheduled interval, or sacrificed at the Week 14 and 53 interim sacrifices, terminal, and recovery sacrifices were sent to Tox Path Inc. for peer-review and results were provided to Covance for inclusion in the final report (see Protocol Deviations for exceptions). After examination, slides were returned to Covance. Statistical Evaluation Levene's test (Levene, 1960) was done to test for variance homogeneity. In the case of heterogeneity of variance at p < 0.05, transformations were used to stabilize the variance. One-way analysis of variance [ANOVA (Winer, 1971)] was used to analyze body weights, body weight changes, food consumption, continuous clinical pathology values, palmitoyl CoA oxidase activities, and organ weight data (Week14 and 53 interim sacrifices only). ANOVA was done on the homogeneous or transformed data. If the ANOVA was significant, Dunnett's t-test (Dunnett, 1964) was used for pairwise comparisons between treated and control groups. Group comparisons (Groups 2 through 6 versus Group 1) were evaluated at the 5.0%, two-tailed probability level. Only data collected on or after the first day of treatment were analyzed statistically. Survival and Tumor Analysis Survival. Evaluations of trend and heterogeneity of survival data were performed using the Cox-Tarone binary regression method using the National Cancer Institute (NCI) Life Table Package (Thomas, Breslow, and Gart, 1977). Week 105 was treated as the end of the study in the NCI package for both sexes. Animals that were sacrificed at scheduled 30 Covance 6329-183 3M T-6295 intervals were censored in the analysis. Continuity-corrected one-sided tail probabilities for trend and two-sided tail probabilities for group comparisons were evaluated at the 5.0% significance level. Nonneoplastic Lesions. Nonneoplastic lesions were chosen for statistical analyses if the incidence in at least one treated group (Groups 2 through 5) was increased or decreased by at least two occurrences over the control group (Group 1). The high recovery group (Group 6) was selected based on the incidence rate (%) if it was increased or decreased by at least two as compared to the control or high-dose (20.0 ppm) group. When there was only one incidence in the high-recovery and none in the other groups, that case was excluded from the statistical evaluation. These were analyzed by the Cochran-Armitage test for trend and the Fisher-Irwin exact test for control versus treatment comparisons (Thakur, Berry, and Mielke, 1985). One-sided tail probabilities for trend and group comparisons were evaluated at the 5.0% significance level and are reported in Text Tables 3 and 4 for males and females, respectively. Neoplastic Lesions. Neoplastic lesions were chosen for statistical analyses using the same criteria as in the case of nonneoplastic lesions. The incidental tumors (i.e., tumors that were not assigned to be the cause of death of the animals by the study pathologist) were analyzed by logistic regression of tumor prevalence (Dinse and Lagakos, 1983). The logistic regression model included linear and quadratic terms in age (week of death), but thequadratic term was eliminated if there was a lack of convergence. The fatal and palpable (superficial) tumors were analyzed by Cox-Tarone binary regression method using the death time or the first palpation time (as applicable) as a surrogate for the tumor onset time. In the case of any particular tumor type where the study pathologist assigned the tumor in question being the cause of death of a subset of the animals and the rest of the animals were assumed to be dead of other competing risks, IARC-type (Peto et al, 1980) cause of death analysis was performed. Specifically, the subset of the tumors which were assigned to be the cause of death by the study pathologist was analyzed by Cox-Tarone logistic regression under life table techniques. The subset, which was considered incidental by the pathologist, was analyzed by logistic regression of tumor prevalences. Tumor types whose cause of death was undetermined, were treated as incidental for statistical evaluation. The score statistics and their respective variances from the above tests were then used to compute the combined evidence as described by Gart et al. (1986). If one tumor in the group belonged to one of the two categories (fatal and incidental) in a test was combined with the other category for the purpose of statistical 31 Covance 6329-183 3M T-6295 analyses. In addition, in the cases where there was lack of convergence for the asymptotic test of the logistic regression method, the exact probability of the significance was obtained by using LogXact-Turbo (1993) and combined with the probability of Cox-Tarone test, if necessary. The benign and malignant neoplastic incidences were evaluated both separately and combined where appropriate. The criteria for combination were based on the work of McConnell et al. (1986). The incidences of multiple-organ findings such as hemangioma, hemangiosarcoma, lipoma, liposarcoma, fibroma, fibrosarcoma, leiomyoma, leiomyosarcoma, endometrial stromal polyp, and endometrial stromal sarcoma were counted by animal, not by tissue type. They were evaluated statistically if they met the selection criterion for the analysis. The statistical results for these cases may be biased because not all the animals were examined for every tissue. One-sided trend and pairwise group comparisons were evaluated at the 5.0% significance level and are shown in Text Tables 5 and 6 for males and females, respectively. In the nonneoplastic analyses, animals sacrificed early (Week 4) were excluded from the denominators. In the statistical evaluations of the neoplastic lesions, animals sacrificed early (during or before Week 14) were discounted from the number of animals at risk. In the neoplastic analyses, the low- (0.5 ppm), mid-(2.0 ppm), mid-high, and high recovery groups that did not have complete histopathology examinations were excluded from statistical analyses. In the text tables, the p-values under Group 1 (control) are for trend analysis and the p -values under Groups 2 (low), 3 (mid), 4 (mid-high), 5 (high) and 6 (high recovery) are for the comparison of that group versus the control group. Group 6 was also compared with Group 5, and the p-values for those comparisons are indicated in the text tables. Trend analysis includes Groups 1, 2, 3, 4, and 5 only. Ordinal dose levels 0, 1, 2, 3, and 4 were used in all analyses for Groups 1, 2, 3, 4, and 5, respectively. Continuity correction was done for all asymptotic tests. The following notations are used throughout this report designating direction and significance of effects. 32 + = Effect in the increased direction - = Effect in the decreased direction * = Significant at p < 0.05 ** = Significant at p < 0.01 E = Exact permutation test NA = Not analyzed Covance 6329-183 3M T-6295 Record Retention The raw data, documentation, records, specimens, protocol, and final report generated as a result of this study will be archived in the storage facilities of Covance-Madison. At least 1 year after submission of the final report, the Covance Archives staff will contact the sponsor. At that time, the sponsor may choose to have the aforementioned materials returned, archived for an additional period of time, or disposed of by Covance; a fee will be charged based on the archive disposition option selected by the sponsor. Raw data stored on magnetic media and the protocol, study correspondence, and the original final report will be retained by Covance. At least 1 year after submission of the final report, any frozen samples will be returned to the sponsor, retained by Covance, or disposed of, and a fee will be charged. Data generated by the sponsor will be archived in the sponsor's storage facilities. Specimens, data, and reports for PCNA evaluation, BrdU immunohistochemistry and electron microscopy will be retained by PAI. RESULTS AND DISCUSSION Dose Analysis Results of the homogeneity, stability, and dose preparation analyses are in Tables 1 through 3. Several homogeneity analyses were conducted on each of the several mixes in an attempt to resolve issues related to the inherent variability and lack of sufficient sensitivity in the analytical method for measuring Perfluorooctane sulfonic acid potassium salt (PFOS; T-6295). Mean values of the homogeneity analyses for the pre-dosing mix done on March 18, 1998, ranged from 119-135% and 107-118% of the theoretical concentrations for the diets containing 0.5 and 20 ppm (PFOS; T-6295), respectively. Mean values of 33 Covance 6329-183 3M T-6295 the homogeneity analyses for the mix done on April 16, 1998, ranged from 96.2-122%, 98.5-107%, 97.6-101%, and 90.0-101% of the theoretical concentrations for the diet containing 0.5, 2, 5, and 20 ppm (PFOS; T-6295), respectively. Mean values of the homogeneity analyses for the mix done on April 22, 1998, ranged from 93.5-103% of the theoretical concentrations for the diet containing 20 ppm (PFOS; T-6295). Mean values of the homogeneity analyses for the mix done on June 18, 1998, ranged from 91.0-175%, 111-130%, 99.6-102%, and 90.5-97.0% of the theoretical concentrations for the diet containing 0.5, 2, 5, and 20 ppm (PFOS; T-6295). These results indicate that the mixing procedure produced a homogeneous distribution of the test material in the dose preparations; although variability generally appeared slightly greater at the lower dietary concentrations. Stability analyses were conducted prestudy in an attempt to resolve issues related to the inherent variability and lack of sufficient sensitivity at levels in the analytical method for measuring (PFOS; T-6295). Results of stability analyses for the pre-dosing mix conducted on March 18, 1998, of samples stored for 28 days at room temperature indicated that the mean concentrations were 78.5, 76.7, 79.8, and 88.1% of the initial concentrations of 0.5, 1, 2, and 20 ppm, respectively. Results of stability analyses for the mix conducted on April 16, 1998, of samples stored for 33 days at room temperature indicated that the mean concentrations were 112.3, 119.6, 113.9, and 126.7% of the initial concentrations of 0.5, 2, 5, and 20 ppm, respectively. The mean concentrations of the dose preparation analyses for all levels ranged from 44.4-635% of the theoretical concentrations (including the reassay and retention sample analyses). The overall mean concentrations within each dose level ranged from 102.3 to 108.7. Inherent variability and lack of sufficient sensitivity at low levels in the analytical method for measuring (PFOS; T-6295) resulted in homogeneity, stability, and routine analysis data that were in many cases outside of the standard limits of +/- 15%. Observation of Animals Survival and Clinical Observations. Clinical observations including mass observations are summarized in Tables 5 and 6; individual data are in Appendix 2. Adjusted survival data are illustrated in Figures 1 and 2 and summarized in Table 4; individual animal fate data are in Appendix 2. 34 Covance 6329-183 3M T-6295 Survival after 104 weeks of treatment was 22.0, 22.0, 34.0, 50.0, 45.0, and 27.0% for males in Groups 1, 2, 3, 4, 5, and 6, respectively, and 50.0, 30.0, 20.0 (after 102 weeks), 34.0, 52.0, and 48.0% for females in Groups 1, 2, 3, 4, 5, and 6, respectively. There was a significant increased trend in survival that occurred in the males that and was due to significant increases in survival in mid-high (5.0 ppm) and high-dose (20.0 ppm) groups as compared to that of the control group. None of the other treated groups in the males revealed any significant differences in survival. No significant trend was noted in survival in females. There was a significant decrease in survival in the mid-dose (2.0 ppm) group and not in the mid-high (5.0 ppm) and high-dose (20.0 ppm) groups as compared to that of the control. There were no clinical observations attributed to administration of the test material. The types of clinical observations noted are commonly observed in laboratory rats as they age on long term safety studies. There was no effect of the test material on the incidence of palpable masses. Body Weights. Body weight and body weight change data are illustrated in Figures 3 and 4 and summarized in Tables 7 and 8; individual body weight data are in Appendix 3. Males given 20 ppm (Groups 5 and 6) had significantly lower mean body weights compared to animals in the control group during Weeks 9 through 37. During recovery the mean body weights for Group 6 males were similar to mean body weights for Group 5 males. Females in Group 5 given 20 ppm had significantly lower body weights compared to animals in the control group during Weeks 3 through 101. Mean body weights for females in Group 6 given 20 ppm were statistically lower than mean body weights for control females during Weeks 3 through 61, but their mean body weights became nearer to the weights of the control females when placed on recovery. At Week 105, mean body weights for surviving males were 98, 108, 103, 101, and 96% of controls for the low-, mid-, and high-dose groups (Groups 5 and 6), respectively. At Week 105, mean body weights for surviving females were 101, 107 (Week 101), 100, 86, and 105% of controls, for the low-, mid-, and high-dose groups (Groups 5 and 6), respectively. Overall mean body weight change was statistically significantly lower for males given 20 ppm diets for Weeks 1 through 15 and Weeks 15 through 29. Mean body weight change was statistically significantly lower for females given 20 ppm for Weeks 1 35 Covance 6329-183 3M T-6295 through 15 and Weeks 29 through 57 (Group 5 only). Mean body weight changes for males and females in Group 6 were significantly increased during the several weeks of recovery. Animals at lower dose levels occasionally had statistically significant decreases in body weight gain; however, these occurrences were inconsistent over time between sexes and were not clearly dose-related. Food Consumption. Food consumption data are illustrated in Figures 5 and 6 and summarized in Table 9; individual data are in Appendix 4. Although not always statistically significant, males given 20 ppm tended to consume less food during Weeks 1 through 24. Food consumption was similar for males given 20 ppm compared to animals given the control material during Weeks 28 through 104. Statistically significantly lower food consumption was noted for females given 20 ppm during Weeks 2 through 36. Food consumption for males and females was similar in all the other treated groups compared to animals given the control material. Test M aterial Consumption. Test material consumption data are summarized in Table 10. Individual test material consumption data are presented in Appendix 4. Animals were fed diets containing 0, 0.5, 2, 5, or 20 ppm T-6295. The amount of test material consumed by animals on a mg/kg of body weight/day basis was as follows. Group 2 3 4 5 6 Dietary Concentrations (ppm) 0.5 2.0 5.0 20.0 20.0 Mean Achieved Dose Levels - Range (mg/kg body weight/day) Males Females 0.015-0.057 0.015-0.052 0.064 - 0.226 0.073 -0.213 0.153 -0.570 0.186 -0.559 0.643 -2.205 0.838 -2.149 0.732- 2.336 1.047 -2.160 Clinical Pathology Hematology, coagulation, clinical chemistry, urinalysis, and urine chemistry data are summarized in Tables 11 through 29; individual data are in Appendix 5. The Pathology Report contains a discussion of the data. 36 Covance 6329-183 3M T-6295 Dietary administration of PFOS for approximately 53 weeks was associated with mildly higher urea nitrogen for males and females fed 5 or 20 ppm; mildly lower glucose for males and females fed 20 ppm; mildly to moderately lower cholesterol for males and females fed 20 ppm; and mildly higher alanine aminotransferase for males fed 20 ppm. None of these effects were considered adverse. The effect on cholesterol was no longer apparent after 104 weeks of treatment. There was no effect on hepatic palmitoyl-CoA oxidase activity. Anatomic Pathology Organ weight data are summarized in Tables 30 through 31; incidences of macroscopic and microscopic observations are summarized in Tables 32 through 41. Individual data are in Appendix 6. The Pathology Report contains a discussion of the data. Terminal body weights in males and females at Week 14 were comparable between control and treated groups. At the Week 14 interim sacrifice, absolute and relative liver weights were significantly increased in the males given 20 ppm. In females given 20 ppm, only the liver-to-body weight percentage was significantly increased. Test material-related histomorphologic changes were limited to the liver in the males given 5 or 20 ppm and in the females given 20 ppm. The changes consisted of hypertrophy of hepatocytes in centrilobular areas in males and females, and midzonal to centrilobular hepatocytic vacuolation. The incidence and severity of the changes tended to be greater in the males. At the Week 53 interim sacrifice, terminal body weights were significantly decreased in the females given 20 ppm. In the males, absolute and relative liver weights were increased in the group receiving 20 ppm. In addition, absolute and relative spleen weights were decreased in males given 20 ppm. There were no clear or consistent gross observations at the Week 53 interim sacrifice that could be attributed to the administration of the test material. At the Week 53 interim sacrifice, centrilobular hepatocytic hypertrophy and vacuolation was increased in incidence and severity in the males given 20 ppm. Generally, in the females given 20 ppm, only centrilobular hypertrophy was seen, and the change was less severe than that noted in the males. In addition, minimal to slight centrilobular hepatocytic pigment was found in the females given 20 ppm. There were no other histomorphologic changes that could be associated 37 Covance 6329-183 3M T-6295 with the administration of the test material. Liver findings in several unscheduled deaths given 20 ppm resembled those seen in animals sacrificed at Week 53. At the terminal sacrifice, the livers of animals given 5 or 20 ppm exhibited a slight increase in macroscopic findings, including enlarged, mottled, diffuse darkened, or focally lightened. Hepatotoxicity, characterized by centrilobular hypertrophy, centrilobular eosinophilic hepatocytic granules, centrilobular hepatocytic pigment, or centrilobular hepatocytic vacuolation was noted in the animals given 5 or 20 ppm. An increase in eosinophilic clear cell altered foci and cystic hepatocellular degeneration was noted in the males given 2, 5, or 20 ppm. The data suggests that the hepatotoxicity did not persist in the recovery-sacrifice animals. In the unscheduled sacrifices between Weeks 54 and 105, animals given 20 ppm had increased hepatocellular centrilobular hypertrophy, eosinophilic hepatocytic granules, and centrilobular hepatocytic pigment were noted. Increased hepatocellular centrilobular hypertrophy was seen in animals given 5 ppm. A significant positive trend was noted in the incidences of liver hepatocellular adenoma for males. This was associated with a significant increase in the high-dose (20.0 ppm) group over the control. The high-dose recovery (20.0 ppm) group animals in this case exhibited a significantly decreased incidence when compared to that of the high-dose (20.0 ppm) group. Significantly increased incidences were observed for thyroid follicular cell adenoma in the high-dose recovery (20.0 ppm) group when compared to either the control or the high-dose (20.0 ppm) group. There was also a significant increase in the combined (adenoma and carcinoma) thyroid follicular cell tumors in the high-dose recovery (20.0 ppm) group animals as compared to that of the high-dose (20.0 ppm) group. None of these cases were associated with any significant trend. Thyroid follicular cell carcinoma did not show any significant effect. There were no other significant neoplastic findings in the males. Significant positive trends were observed in the incidences of hepatocellular adenoma and hepatocellular combined adenoma and carcinoma in the females. These cases were associated with significant increases in the high-dose (20.0 ppm) group as compared to the control. All these tumors were incidental and noted at the terminal sacrifice. A 38 Covance 6329-183 3M T-6295 marginally significant increase for thyroid follicular cell adenoma and carcinoma combined was observed in the mid-high (5.0 ppm) group but not in the high-dose (20.0 ppm) group as compared to the control group. In fact, thyroid C-cell adenoma and thyroid C-cell combined adenoma and carcinoma actually showed significant decreases in the high-dose (20.0 ppm) group as compared to the control. There were significant negative trends in the incidences of mammary fibroadenoma and mammary combined adenoma and fibroadenoma. These cases were associated with significantly lower incidences in the respective high-dose (20.0 ppm) group when compared to that of the control. However, the low-dose (0.5 ppm) group animals for these cases exhibited significant increases over the control. A significant decreased incidence was found for the high-dose recovery (20.0 ppm) group in the incidences of mammary carcinoma as compared to the high-dose (20.0 ppm) group. There was no other significant group effect in mammary carcinoma. There was a marginal significant negative trend for the mammary combined fibroadenoma/adenoma/carcinoma. However, the low- (0.5 ppm) and mid-high (5.0 ppm) groups for this case exhibited significantly increased incidences over the control. There were no other significant neoplastic findings in the females. Cell Proliferation Results There were no statistically significant increases in cell proliferation as measured by PCNA at weeks 4 and 14, or by bromodeoxyuridine (BrdU) immunohistochemistry at week 53. However, two out of five females in each of the mid-high and high dose groups showed a very mild, but biologically significant response (i.e. had a proliferative index that was twice the mean of the controls and greater than that of the highest control) at week 4. The cell proliferation response at week four was transient in nature as it was not observed at week 14, and was considered to be test compound-related. Electron Microscopy Results Liver samples were obtained from each of the 20 rats during necropsy at terminal sacrifice for examination by transmission electron microscopy (TEM). Liver samples were assessed for ultrastructural changes that may be related to chronic toxicity of the test material when administered in the diet. Electron microscopic evaluation identified mild to moderate changes related to treatment. Mild to moderate smooth endoplasmic 39 Covance 6329-183 3M T-6295 reticulum hyperplasia and minimal to mild hepatocellular hypertrophy were the prominent features found to be different between the high dose group and the control animals. Other changes included a slight increase in the amount of glycogen in treated animals compared with controls. Characterization of Tumor Incidence Outcome In males, hepatocellular adenoma was increased for trend (p = 0.0276) and pair-wise against control for the 20 ppm dose group (7/60 20 ppm vs. 0/60 control, p = 0.0456). Hepatocellular adenoma was not observed in the 20 ppm recovery group, which was significant compared to the 20 ppm dose group (0/40 20 ppm recovery group vs. 7/60 200 ppm dose group, p = 0.0240). Overall, the response in terms of hepatocellular adenoma can be considered weak at the 20 ppm dose level. Thyroid follicular cell adenoma was increased pair-wise against control for the 20 ppm recovery group (9/39 20 ppm recovery group vs. 3/60 control, p = 0.0280). Although this finding is outside the range of recent historical control values in this laboratory, there were no other indications of thyroid abnormality. In females, hepatocellular adenoma was increased for trend (p = 0.0153) and pair-wise against control for the 20 ppm dose group (5/60 20 ppm dose group vs. 0/60 control, p = 0.0386). Overall, the response in terms of hepatocellular adenoma can be considered weak at the 20 ppm dose level. The only hepatocellular carcinoma observed in the study was at the high dose. Hepatocellular adenoma/carcinoma was increased for trend (p = 0.0057) and pair-wise against control for the 20 ppm dose group (6/60 20 ppm vs. 0/60 control, p = 0.0204). Mammary fibroadenoma was decreased for trend (p = 0.0152) and pair-wise against control for the 20 ppm dose group (11/60 20 ppm vs. 20/60 control, p = 0.0235). 40 CONCLUSION Covance 6329-183 3M T-6295 The chronic toxicity and carcinogenicity of the test material, Perfluorooctane sulfonic acid potassium salt (PFOS; T-6295) was assessed following dietary admixture to male and female Crl:CD(SD)IGS BR rats at dose levels of 0.5, 2, 5, or 20 ppm. Based on anatomic pathology findings in the liver, the no-observable-adverse-effect level for Perfluorooctane sulfonic acid potassium salt (PFOS; T-6295) is considered to be 2 ppm. 41 SIGNATURES Covance 6329-183 3M T-6295 42 Covance 6329-183 3M T-6295 REFERENCES Dunnett, C. W., "New Tables for Multiple Comparisons with a Control," Biometrics, 20:482-491 (1964). Levene, H., "Robust Tests for Equality of Variances," Contributions to Probability and Statistics, (eds.) I. Olkin et al., Ch. 25, pp. 278-292, Stanford University Press: Stanford, California (1960). Winer, B. J., "Design and Analysis of Single-Factor Experiments," Statistical Principles in Experimental Design, Second Ed., Ch. 3, pp. 149-260, McGraw-Hill: New York, New York (1971a). Winer, B. J., "Analysis of Covariance," Statistical Principles in Experimental Design, Second Ed., Ch. 10, pp. 752-812, McGraw-Hill: New York, New York (1971b). 43 PATHOLOGY REPORT Covance 6329-183 3M T-6295 SUMMARY The purpose of this study was to assess the chronic toxicity and carcinogenicity of the test material, Perfluorooctane Sulfuric Acid Potassium Salt (PFOS) when administered in the diet to rats for at least 104 weeks. The test material was administered at dose levels of 0.5, 2, 5, and 20 ppm. Dietary administration of PFOS for approximately 53 weeks was associated with mildly higher urea nitrogen for males and females fed 5 or 20 ppm; mildly lower glucose for males and females fed 20 ppm; mildly to moderately lower cholesterol for males and females fed 20 ppm; and mildly higher alanine aminotransferase for males fed 20 ppm. None of these effects were considered adverse. The effect on cholesterol was no longer apparent after 104 weeks of treatment. There was no effect on hepatic palmitoyl-CoA oxidase activity. Terminal body weights in males and females at Week 14 were comparable between control and treated groups. At the Week 14 interim sacrifice, absolute and relative liver weights were significantly increased in the males given 20 ppm. In females given 20 ppm, only the liver-to-body weight percentage was significantly increased. Test material-related histomorphologic changes were limited to the liver in the males given 5 or 20 ppm and in the females given 20 ppm. The changes consisted of hypertrophy of hepatocytes in centrilobular areas in males and females, and midzonal to centrilobular hepatocytic vacuolation. The incidence and severity of the changes tended to be greater in the males. At the Week 53 interim sacrifice, terminal body weights were significantly decreased in the females given 20 ppm. In the males, absolute and relative liver weights were increased in the group receiving 20 ppm. In addition, absolute and relative spleen weights were decreased in males given 20 ppm. There were no clear or consistent gross observations at the Week 53 interim sacrifice that could be attributed to the administration of the test material. At the Week 53 interim sacrifice, centrilobular hepatocytic hypertrophy and vacuolation was increased in incidence and severity in the males given 20 ppm. Generally, in the females given 20 ppm, only centrilobular 44 Covance 6329-183 3M T-6295 hypertrophy was seen, and the change was less severe than that noted in the males. In addition, minimal to slight centrilobular hepatocytic pigment was found in the females given 20 ppm. There were no other histomorphologic changes that could be associated with the administration of the test material. Liver findings in several unscheduled deaths given 20 ppm resembled those seen in animals sacrificed at Week 53. At the terminal sacrifice, the livers of animals given 5 or 20 ppm exhibited a slight increase in macroscopic findings, including enlarged, mottled, diffuse darkened, or focally lightened. Hepatotoxicity, characterized by centrilobular hypertrophy, centrilobular eosinophilic hepatocytic granules, centrilobular hepatocytic pigment, or centrilobular hepatocytic vacuolation was noted in the animals given 5 or 20 ppm. An increase in eosinophilic clear cell altered foci and cystic hepatocellular degeneration was noted in the males given 2, 5, or 20 ppm. The data suggests that the hepatotoxicity did not persist in the recovery-sacrifice animals. In the unscheduled sacrifices between Weeks 54 and 105, animals given 20 ppm had increased hepatocellular centrilobular hypertrophy, eosinophilic hepatocytic granules, and centrilobular hepatocytic pigment were noted. Increased hepatocellular centrilobular hypertrophy was seen in animals given 5 ppm. A complete discussion of statistically significant differences in the incidence of neoplasms may be found in the section entitles "Statistical Report of Survival, Nonneoplastic, and Neoplastic Lesions." METHODS Six groups of Crl:CD(SD)IGS BR rats were studied using the following study design. 45 Group 1 (Control) 2 (Low) 3 (Mid) 4 (Mid-High) 5 (High) 6 (High Recovery) Number of Animals Male Female 70 70 60 60 60 60 60 60 70 70 40 40 Covance 6329-183 3M T-6295 Dietary Levels (ppm T-6295) 0 0.5 2.0 5.0 20.0 20.0 The animals in Group 6 were designated as recovery animals and were treated for 52 weeks, then placed in recovery for an additional 52 weeks. During Weeks 4, 14, 27, and 53, blood and urine were collected for hematology, clinical chemistry, urinalysis, and urine chemistry tests from 10 animals/sex in Groups 1 through 5. Blood was collected for cholesterol and triglyceride determinations from all animals prior to the terminal sacrifice during Weeks 105 and 106 (Week 103 for females in Group 3) and the recovery sacrifice (Group 6) during Weeks 105 and 106. Blood films were also prepared for animals at the terminal and recovery sacrifices. Five animals/sex in Groups 1 through 5 were sacrificed during Week 4; livers were collected and weighed. A portion of the liver was shipped to the sponsor for PFOS analysis and mitochondrial activity, a portion was shipped to Pathology Associates International (PAI) for hepatocellular proliferation rate measurements by proliferation cell nuclear antigen (PCNA), and a third portion was used for determination of palmitoyl-CoA oxidase activity. During Weeks 14 and 53, necropsies were performed on five and 10 animals/sex, respectively, in Groups 1 through 5. Surviving females in Group 3 were sacrificed and necropsied during Week 103 because of reduced survival. The remaining animals in Groups 1 through 6 were sacrificed and necropsied during Weeks 105 and 106. At necropsy, macroscopic observations were recorded, organ weights were obtained (Weeks 14 and 53), and tissues were placed in fixative as specified by the protocol. In addition, liver samples were collected for PFOS analysis (Weeks 14, 53, 103, 105, and 106), hepatocellular proliferation rate measurement [by PCNA at Week 14 and by bromodeoxyuridine (BrdU) immunohistochemistry at Week 53], and palmitoyl-CoA oxidase determination (Week 14). A section of duodenum was also collected at Week 53 for BrdU immunohistochemistry. At the terminal and recovery sacrifices, sections of heart and liver from 10 animals/sex in Groups 1, 4, 5, and 6 were preserved in 2.0% parafomaldehyde/2.5% gluteraldehyde in 0.1M phosphate buffer, processed, and embedded in epoxy resin for possible future electron microscopy. 46 Covance 6329-183 3M T-6295 At unscheduled necropsies for animals that died or were sacrificed because of poor health, macroscopic observations were recorded, and tissues were placed in fixative, but organ weights were not obtained. Microscopic examinations were done on selected tissues (adrenals, brain, eyes, kidney, liver, mesenteric lymph node, pancreas, spleen, testes, and ovaries) from the animals necropsied during Week 14 and on all tissues from the animals in Groups 1 and 5 that were necropsied during Weeks 53, 105, and 106. In addition, microscopic examinations were done on the tissues from animals that died or were sacrificed due to poor health. Macroscopic lesions, liver, lung, kidneys, pancreas, thyroid, testes, and mammary gland (females) were examined for animals in Groups 2, 3, 4, and 6 that were sacrificed during Weeks 103, 105, and 106. Sections of liver were stained with Oil Red O and examined from five terminally sacrificed males and females in Groups 1 and 5. Also, urinary bladder was examined microscopically from all terminally sacrificed animals in Groups 2, 3, and 4, and from all animals at the recovery sacrifice. Statistically significant differences cited in the Results and Discussion section are based on comparisons between the control and treated groups. RESULTS AND DISCUSSION Clinical Pathology Weeks 4,14, 27, and 53. There were relatively few statistically significant or otherwise notable differences for clinical pathology results between the control and treated groups. Those differences that were the most consistent over time were considered to be associated with administration of the test material. These included mildly higher urea nitrogen for males and females fed 5 or 20 ppm; mildly lower glucose for males and females fed 20 ppm; mildly to moderately lower cholesterol for males and females fed 20 ppm; and mildly higher alanine aminotransferase for males fed 20 ppm. There were no correlative microscopic renal findings for the minor change in urea nitrogen. The findings for alanine aminotransferase, and possibly for glucose and cholesterol, were likely associated with the histopathological findings of hepatocellular hypertrophy and vacuolation. None of the clinical pathology effects were considered adverse. 47 Covance 6329-183 3M T-6295 Of uncertain relationship to the test material were statistically higher urea nitrogen for males fed 2 ppm and statistically lower glucose for females fed 2 or 5 ppm at Week 53. These findings were not as consistent over time as those considered to be effects of the test material, and the magnitudes of these differences were very small. All other statistically significant differences for clinical pathology results between the control and treated groups were considered incidental. Most of these differences did not affect animals fed the highest dose level, and none of these differences were consistent over time. Week 103 (Day 719; Group 3 Females) and Week 105. As expected for rats of this age, the cholesterol and triglyceride results were extremely variable, especially for the males. Because of this naturally-occurring variability, the effect on cholesterol observed at earlier intervals was no longer clearly apparent. Although males and females fed 20 ppm had lower mean cholesterol concentrations than those of the other groups, the marked individual variability made it impossible to definitively identify a test material-related effect on cholesterol at Week 105. The recovery males (Group 6) had a mean cholesterol concentration similar to that of the control males, but the recovery females had a mean cholesterol concentration that was similar to the females in Group 5. Once again, the marked individual variability for cholesterol obscured the significance of these findings. Anatomical Pathology Week 14, Terminal Body Weight and Organ Weights. Terminal body weights were comparable between control and treated groups. Absolute and relative liver weights were statistically significantly increased in the males given 20 ppm. In females given 20 ppm, only the liver-to-body weight percentage was significantly increased. The absolute spleen weight was significantly decreased in the females given 20 ppm, as was the absolute lung weight in females given 2, 5, or 20 ppm. Spurious, significant increases in left thyroid/parathyroid-to-body weight percentages were seen in females given 5 or 20 ppm. Macroscopic Observations. There were no macroscopic observations that could be attributed to the administration of the test material. 48 Covance 6329-183 3M T-6295 Microscopic Observations. Test material-related histomorphologic changes were limited to the liver in the males given 5 or 20 ppm and in the females given 20 ppm. The changes consisted of hypertrophy of hepatocytes in centrilobular areas and midzonal to centrilobular hepatocytic vacuolation. The incidence and severity of the changes tended to be greater in the males. There were no potentially test material-related lesions in the remaining tissues examined. Week 53 Interim Sacrifice, Body and Organ Weights. In the females, terminal body weights were significantly decreased in the group given 20 ppm. Terminal body weights in the males were comparable between the control and treated group. In the males, absolute and relative liver weights were increased in the group receiving 20 ppm. In addition, absolute and relative spleen weights were decreased in males given 20 ppm. Significantly decreased left thyroid/parathyroid weights were considered to be spurious due to the absence of a contralateral effect. In view of the significant decrease in body weight for females given 20 ppm, significant increases in organ-to-body weight percentages for brain, kidney, liver, and spleen may be of no toxicological importance. Decreased absolute weights in the left adrenal gland and bilateral adrenal-to-brain weight ratios may also represent changes secondary to the body weight loss. Macroscopic Observations. There were no clear or consistent gross observations at the Week 53 interim sacrifice that could be attributed to the administration of the test material. Microscopic Observations. Centrilobular hepatocytic hypertrophy and vacuolation were increased in incidence and severity in the males given 20 ppm. Generally, in the females given 20 ppm, only centrilobular hypertrophy was seen, and the change was less severe than that noted in the males. In addition, minimal to slight centrilobular hepatocytic pigment was found in the females given 20 ppm. There were no other histomorphologic changes that could be associated with the administration of the test material. 49 Covance 6329-183 3M T-6295 Unscheduled Deaths Through Week 53 Large, mottled, or diffusely dark livers were noted in 2/3 males and 1/1 females given 20 ppm. There were no other gross observations that could be attributed to the administration of the test material. Microscopically, several animals given 20 ppm exhibited liver changes that were similar or related to those seen at the Week 53 interim sacrifice. Terminal Sacrifice Macroscopic Observations. The livers of animals given 5 or 20 ppm exhibited a slight increase in macroscopic findings, including enlarged, mottled, diffuse darkened, or focally lightened. Microscopic Observations. Hepatotoxicity, characterized by centrilobular hypertrophy, centrilobular eosinophilic hepatocytic granules, centrilobular hepatocytic pigment, or centrilobular hepatocytic vacuolation was noted in animals given 5 or 20 ppm. An increase in eosinophilic clear cell altered foci and cystic hepatocellular degeneration was noted in the males given 2, 5, or 20 ppm. There were no clear differences in the Oil Red O stained sections between Groups 1 and 5. Recovery Sacrifice Macroscopic Observations. There were no consistent gross findings in the recovery sacrifice animals that could be attributed to the test material. Microscopic Observations. The data suggests that the hepatotoxicity did not persist in the recovery-sacrifice animals. Unscheduled Deaths Weeks 54-105 Macroscopic Observations. There were no consistent gross findings in the recovery sacrifice animals that could be attributed to the test material. Microscopic Observations. Liver findings of the died-on-test or moribund sacrifice animals were generally similar to those seen in the terminally-sacrificed animals. Increased hepatocellular centrilobular hypertrophy, eosinophilic hepatocytic granules, 50 Covance 6329-183 3M T-6295 and centrilobular hepatocytic pigment were noted in the animals given 20 ppm. Increased hepatocellular centrilobular hypertrophy was seen in animals given 5 ppm. Neoplasm Analysis - All Animals A complete discussion of statistically significant differences in the incidence of neoplasms may be found in the section entitles "Statistical Report of Survival, Nonneoplastic, and Neoplastic Lesions." 51 Covance 6329-183 52 Colorado Pathology Services, Inc. Covance 6329-183 3M T-6295 Project Number: P-1934 Introduction Apeer reviewofthe histopathology portion ofthe study entitled: 104-WeekDietaryChronic Toxicity and Carcinogenicity Study with Perfluorooctane Sulfonic Acid Potassium Salts (PFOS; T-6295) inRats was conducted at Covance, Madison, Wisconsin, By Donald N. Kitchen, D.V.M., PhD of Colorado Pathology Services, Inc., Collbran, Colorado. The tissues had been previously examined and the data tabulated by pathologist, Dr. Richard Alsacker, at Covance. Experimental Design A summary of the experimental design for the study is presented below: Group 1 (Control)3'b'c 2 (Low)b 3 (Mid)b 4 (Mid-High)b 5(High)b'c 6 (High Recovery)d Number ofAnimals Male 70 60 60 60 70 40 Female 70 60 60 60 70 40 Dietary Levels (ppm T-6295) 0 0.5 2.0 5.0 20.0 20.0 a. The control animals received the basal diet only. b. Five animals/sex in Groups 1 and 5 were sacrificed at Weeks 4 and 14 for hepatocellular proliferation rate measurements, biochemical analysis. c. Ten animals/sex in Groups 1 and 5 were designated as interim sacrifice animals. These animals were sacrificed after at least 52 weeks oftreatment. d. Animals in Group 6 were treated for at least 52 weeks, then treatment will be discontinued, and the animals were observed for reversibility, persistence, or delayed occurrence of toxic effects for at least 52 weeks posttreatment. During recovery, the animals received basal diet only. 2 53 Colorado Pathology Services, Inc. Covance 6329-183 3M T-6295 Project Number: P-1934 Methods The peer reviewas conducted consisted oftwo tasks. The first taskwas the complete review ofall slides and confirmation ofall diagnoses for 10%ofthe rats inthe control andhighdose (Groups 1and 5) includingthe recovery (Group 6) ofstudy 6329-183. The animals for complete reviewwere selected by Covance and included the following: Males Control Group 5 C92503 C92753 C92505 C92757 C92510 C92761 C92513 C92762 C92514 C92774 C92518 C92775 C92541 C92776 Main Study Females Control Group 5 C92866 C93113 C92867 C93115 C92868 C93124 C92869 C93125 C92871 C93133 C92873 C93135 C92874 C93137 -- Recovery Animals 10%-- Males Females C92821 C93181 C92823 C93182 C92827 C93183 C92833 C93184 54 Colorado Pathology Services, Inc. Covance 6329-183 3M T-6295 Project Number: P-1934 The second task consisted of a review of livers from all animals and thyroid glands and pancreas from all male animals, suspected target tissues. Results Allthematerialsnecessaryforthis reviewwerereadilyavailable andwell organized. Relevant in-life data and necropsy data were available to the reviewing pathologist. All slides required to be examined bythe reviewing pathologist, were present and were considered to be ofgood quality. The quality ofthe histotechnique and the hematoxylin and eosin (H&E) staining were also considered to be good. No tissue artifacts were present that interfered with the slide evaluation and the stain providedgood balancebetween nuclear and cytoplasmic staining. All slideswere adequatelylabeled. Dr. Richard Alsacker the study pathologist, and Marcia J. Holland, histology supervisor, were especially helpful in providing the reviewing pathologist with materials and information needed to complete the peer review. The differences noted during this review were examined and discussed by the study pathologist, the reviewing pathologist and the 3Mpathologist (Dr. Marvin Case) utilizing a multiple headed microscope. All differences noted duringthis reviewwere resolved withcomplete agreement concerning the final diagnoses. Summary The excellent organization of all materials prior to the arrival of the reviewing pathologist greatly aided and simplified this peer review. The slides were well identified, organized by task and ofexcellent quality. All differences noted during this reviewwere resolved with complete agreement concerning the final diagnoses. Donald N. Kitchen, D.V.M., Ph.D. Veterinary Pathologist Diplomate: ACVP 4 55 Covance 6329-183 3M T-6295 STATISTICAL REPORT OF SURVIVAL, NONNEOPLASTIC, AND NEOPLASTIC LESIONS Methods Survival. Evaluations of trend and heterogeneity of survival data were performed using the Cox-Tarone binary regression method using the National Cancer Institute (NCI) Life Table Package (Thomas, Breslow, and Gart, 1977). Week 105 was treated as the end of the study in the NCI package for both sexes. Animals that were sacrificed at scheduled intervals were censored in the analysis. Continuity-corrected one-sided tail probabilities for trend and two-sided tail probabilities for group comparisons were evaluated at the 5.0% significance level, as reported in Text Tables 1 and 2. Kaplan-Meier product limit survival curves are presented in Text Figures 1 and 2 for males and females, respectively. Nonneoplastic Lesions. Nonneoplastic lesions were chosen for statistical analyses if the incidence in at least one treated group (Groups 2 through 5) was increased or decreased by at least two occurrences over the control group (Group 1). The high recovery group (Group 6) was selected based on the incidence rate (%) if it was increased or decreased by at least two as compared to the control or high-dose (20.0 ppm) group. When there was only one incidence in the high-recovery and none in the other groups, that case was excluded from the statistical evaluation. These were analyzed by the Cochran-Armitage test for trend and the Fisher-Irwin exact test for control versus treatment comparisons (Thakur, Berry, and Mielke, 1985). One-sided tail probabilities for trend and group comparisons were evaluated at the 5.0% significance level and are reported in Text Tables 3 and 4 for males and females, respectively. Neoplastic Lesions. Neoplastic lesions were chosen for statistical analyses using the same criteria as in the case of nonneoplastic lesions. The incidental tumors (i.e., tumors that were not assigned to be the cause of death of the animals by the study pathologist) were analyzed by logistic regression of tumor prevalence (Dinse and Lagakos, 1983). The logistic regression model included linear and quadratic terms in age (week of death), but the quadratic term was eliminated if there was a lack of convergence. The fatal and palpable (superficial) tumors were analyzed by Cox-Tarone binary regression method using the death time or the first palpation time (as applicable) as a surrogate for the tumor onset time. In the case of any particular tumor type where the study pathologist assigned the tumor in question being the cause of death of a subset of the animals and the rest of the animals were assumed to be dead of other competing risks, IARC-type (Peto et al, 56 Covance 6329-183 3M T-6295 1980) cause of death analysis was performed. Specifically, the subset of the tumors which were assigned to be the cause of death by the study pathologist was analyzed by Cox-Tarone logistic regression under life table techniques. The subset, which was considered incidental by the pathologist, was analyzed by logistic regression of tumor prevalences. Tumor types whose cause of death was undetermined, were treated as incidental for statistical evaluation. The score statistics and their respective variances from the above tests were then used to compute the combined evidence as described by Gart et al. (1986). If one tumor in the group belonged to one of the two categories (fatal and incidental) in a test was combined with the other category for the purpose of statistical analyses. In addition, in the cases where there was lack of convergence for the asymptotic test of the logistic regression method, the exact probability of the significance was obtained by using LogXact-Turbo (1993) and combined with the probability of Cox-Tarone test, if necessary. The benign and malignant neoplastic incidences were evaluated both separately and combined where appropriate. The criteria for combination were based on the work of McConnell et al. (1986). The incidences of multiple-organ findings such as hemangioma, hemangiosarcoma, lipoma, liposarcoma, fibroma, fibrosarcoma, leiomyoma, leiomyosarcoma, endometrial stromal polyp, and endometrial stromal sarcoma were counted by animal, not by tissue type. They were evaluated statistically if they met the selection criterion for the analysis. The statistical results for these cases may be biased because not all the animals were examined for every tissue. One-sided trend and pairwise group comparisons were evaluated at the 5.0% significance level and are shown in Text Tables 5 and 6 for males and females, respectively. In the nonneoplastic analyses, animals sacrificed early (Week 4) were excluded from the denominators. In the statistical evaluations of the neoplastic lesions, animals sacrificed early (during or before Week 14) were discounted from the number of animals at risk. In the neoplastic analyses, the low- (0.5 ppm), mid-(2.0 ppm), mid-high (5.0 ppm), and high recovery (20 ppm) groups that did not have complete histopathology examinations were excluded from statistical analyses. In the text tables, the p-values under Group 1 (control) are for trend analysis and the p -values under Groups 2 (low), 3 (mid), 4 (mid-high), 5 (high) and 6 (high recovery) are for the comparison of that group versus the control group. Group 6 was also compared with Group 5, and the p-values for those comparisons are indicated in the text tables. 57 Covance 6329-183 3M T-6295 Trend analysis includes Groups 1, 2, 3, 4, and 5 only. Ordinal dose levels 0, 1, 2, 3, and 4 were used in all analyses for Groups 1, 2, 3, 4, and 5, respectively. Continuity correction was done for all asymptotic tests. The following notations are used throughout this report designating direction and significance of effects. + = Effect in the increased direction - = Effect in the decreased direction * = Significant at p < 0.05 ** = Significant at p < 0.01 E = Exact permutation test NA = Not analyzed Results and Discussion Survival. As indicated in Text Table 1, a significant negative trend (p = 0.0005) for decreased mortality occurred in for the males and was associated with significant decreases in mortality in mid-high- (5.0 ppm) and high-dose (20.0 ppm) groups as compared to that of the control group (p = 0.0115 and 0.0292, respectively). None of the other treated groups in the males revealed any significant differences in mortality. As indicated in Text Table 2, no significant trend was noted in mortalilty in females. There was a significant increase in mortality in the mid-dose (2.0 ppm) group (p = 0.0015) and not in the mid-high (5.0 ppm) and high-dose (20.0 ppm) groups as compared to that of the control. Nonneoplastic Lesions. The liver, pancreas, and thyroid in both sexes have been identified as appropriate to perform statistical analysis for nonneoplastic lesions. As indicated in Text Table 3, there were significant positive trends in the incidences of hepatocellular centrilobular hypertrophy, liver eosinophilic centrilobular granular cytoplasm, and hepatocellular midzonal/centrilobular vacuolation (p < 0.01 for all the cases) for males. These cases were associated with significant increases in the high-dose (20.0 ppm) group (p < 0.01 for all the cases) when compared to those of the control group. In addition, there were significantly increased incidences in the mid- (2.0 ppm) and mid-high-dose (5.0 ppm) groups for hepatocellular centrilobular hypertrophy 58 Covance 6329-183 3M T-6295 (p = 0.0415 and 0.0000, respectively) and the mid-high-dose (5.0 ppm) group for hepatocellular midzonal/centrilobular vacuolation (p = 0.0024) as compared to the control in this sex. However, the male high-dose recovery (20.0 ppm) group animals in all these cases exhibited significantly decreased incidences over the high-dose (20.0 ppm) group (p < 0.01 for all the cases), implying that the effect may be transient (reversible) in nature. Significant positive trends were also noted for hepatocellular centrilobular pigment and liver individual hepatocyte necrosis (p = 0.0006 and 0.0106, respectively) in association with significant increases in the respective high-dose (20.0 ppm) group over the control (p = 0.0139 and 0.0224, respectively). A significant positive trend was detected for cystic degeneration in association with significantly increased incidences in all the dose groups as compared to the control (p < 0.01 for Groups 2 through 6 vs. 1, respectively ). Furthermore, a significant positive trend without any significant group effect was noted for liver degeneration/necrosis centrilobular (p = 0.0132). Marginally significant increases over the control were observed in the mid-high-dose (5.0 ppm) group incidences for hepatocellular altered basophilic focus and liver coagulative necrosis (p = 0.0352 and 0.0351, respectively) and in the mid-dose (2.0 ppm) group for pancreatic interstitial fat infiltration (p = 0.0133) without any significant trends. As indicated in Text Table 4, there were significant positive trends in the incidences of hepatocellular centrilobular hypertrophy and liver eosinophilic centrilobular granular cytoplasm (p < 0.01 for both cases) for the females. These cases were associated with significant increases in respective mid-high (5.0 ppm) and high-dose (20.0 ppm) groups (p < 0.01 for all the cases) when compared to that of the control group. Significant positive trends were also noted for hepatocellular centrilobular pigment and liver individual hepatocyte necrosis (p = 0.0000 and 0.0359, respectively). These cases were associated with significant increases in the respective high-dose (20.0 ppm) group (p = 0.0000 and 0.0500, respectively) when compared to that of the control group. However, the high-dose recovery (20.0 ppm) group animals in all the above cases exhibited significantly decreased incidences over the high-dose (20.0 ppm) group (p < 0.01 for all the cases, except for liver individual hepatocyte necrosis (p = 0.0329) implying that the effect may be transient in nature. A significant positive trend in association with a significant increase in the high-dose (20.0 ppm) group was found for lymphohistiocytic infiltration in the liver (p = 0.0080 for trend and 0.0038 for high-dose versus control). A significant positive trend was also noted for cystic degeneration in the liver (p = 0.0187). However, no significant group comparison was detected. In addition, there was a significant positive trend in the incidences of pigmented macrophage 59 Covance 6329-183 3M T-6295 infiltration in the liver (p = 0.0000). This was associated with significant increases in the high-dose (20.0 ppm) and high-dose recovery (20.0 ppm) groups as compared to the control (p = 0.0000 and 0.0147, respectively). However, the high-dose recovery (20.0 ppm) group animals exhibited significantly decreased incidences over the high-dose (20.0 ppm) group (p = 0.0383). A marginally significant positive trend was noted for Zymogen decreased granules in the pancreas (p = 0.0488). This was associated with significant increases in all dosed groups (p = 0.0093, 0.0011, 0.0329, 0.0127, and 0.0030 for Group 2 through 6 versus 1, respectively) when compared to the control. Significant increases were also observed over the control in the mid-dose (2.0 ppm) incidences of hepatocellular periportal vacuolation (p = 0.0358) and the low-dose (0.5 ppm) incidences of liver hematopoiesis extramedullary (p = 0.0.0352). However, no significant trends were associated with them. A significant negative trend was observed for hepatocellular hypertrophy periportal (p = 0.0026). This was associated with a significant decrease in the high-dose (20.0 ppm) group (p = 0.0127) as compared to that of the control The high-dose recovery (20.0 ppm) animals in this case exhibited significantly increased incidences (p = 0.0344) when compared to the high-dose (20.0 ppm) group. Significant negative trends were also noted for angiectasis and congestion in the liver (p = 0.0231 and 0.0451, respectively) and islet cell hyperplasia in the pancreas (p=0.0372). However, no significant group comparisons were found for these cases. Neoplastic Lesions. As indicated in Text Table 5, a significant positive trend was noted in the incidences of liver hepatocellular adenoma for males (p = 0.0276). This was associated with a significant increase in the high-dose (20.0 ppm) group over the control (p = 0.0456). The high-dose recovery (20.0 ppm) group animals in this case exhibited a significantly decreased incidence when compared to that of the high-dose (20.0 ppm) group (p = 0.0240). Significantly increased incidences were observed for thyroid follicular cell adenoma in the high-dose recovery (20.0 ppm) group when compared to either the control or the high-dose (20.0 ppm) group (p= 0.0280 and 0.0121, respectively). There was also a significant increase in the combined (adenoma and carcinoma) thyroid follicular cell tumors in the high-dose recovery (20.0 ppm) group animals as compared to that of the high-dose (20.0 ppm) group (p = 0.0125). None of these cases were associated with any significant trend. Thyroid follicular cell carcinoma did not show any significant effect. There were no other significant neoplastic findings in the males. 60 Covance 6329-183 3M T-6295 As indicated in Text Table 6, significant positive trends were observed in the incidences of hepatocellular adenoma (p = 0.0153) and hepatocellular combined adenoma and carcinoma (p = 0.0057) in the females. These cases were associated with significant increases in the high-dose (20.0 ppm) group as compared to the control (p < 0.05 for both cases). All these tumors were incidental and noted at the terminal sacrifice. A marginally significant increase for thyroid follicular cell adenoma and carcinoma combined was observed in the mid-high (5.0 ppm) group (p = 0.0471) but not in the high-dose (20.0 ppm) group as compared to the control group. In fact, thyroid C-cell adenoma and thyroid C-cell combined adenoma and carcinoma actually showed significant decreases in the high-dose (20.0 ppm) group as compared to the control (p = 0.0336 for both cases). There were significant negative trends in the incidences of mammary fibroadenoma and mammary combined adenoma and fibroadenoma (p < 0.05 for both cases). These cases were associated with significantly lower incidences in the respective high-dose (20.0 ppm) group (p < 0.05 for both cases) when compared to that of the control. However, the low-dose (0.5 ppm) group animals for these cases exhibited significant increases over the control (p < 0.05 for both cases). A significant decreased incidence was found for the high-dose recovery (20.0 ppm) group in the incidences of mammary carcinoma (p = 0.0161) as compared to the high-dose (20.0 ppm) group. There was no other significant group effect in mammary carcinoma. There was a marginal significant negative trend for the mammary combined fibroadenoma/adenoma/carcinoma (p = 0.0482). However, the low- (0.5 ppm) and mid-high (5.0 ppm) groups for this case exhibited significantly increased incidences over the control (p = 0.0474 and 0.0066, respectively). There were no other significant neoplastic findings in the females. References Dinse, G.E. and Lagakos, S.W., "Regression analysis of tumor prevalence data," Journal o fRoyal Statistical Society Series C (Applied Statistics), 32: 236-248 (1983). Gart, J.J., Krewski, D., Lee, P.N., Tarone, R.E., and Wahrendorf, J., Statistical Methods in Cancer Research: The Design and Analysis o fLong-Term Animal Experiments, IARC Scientific Publications, Vol. 3, No. 79, Oxford University Press, New York (1986). LogXact-Turbo, Cytel Software Corporation, Version 1.1, Cambridge, Massachusetts, 1993. 61 Covance 6329-183 3M T-6295 McConnell, E.E., Solleveld, H.A., Swenberg, J.A., and Boorman, G.A., "Guidelines for combining neoplasms for evaluation of rodent carcinogenicity studies,"Journal of National Cancer Institute, 76:283-289 (1986). Peto, R., Pike, M.C., Day, N.E., Gray, R.G., Lee, P.N., Parish, S., Peto, J., Richards, S., and Wahrendorf, J., "Guidelinesfor simple, sensitive significance testsfor carcinogenic effects in long-term animal experiments, in Long-term and Short-term Screening Assays for Carcinogens: A Critical Appraisal," Lyon: International Agency for Cancer Research (IARC), 311-426 (1980). Thakur, A.K., K.J. Berry, and Mielke, P.W., Jr., "A FORTRAN program for testing trend and homogeneity in proportions," Computer Programs in Biomedicine, 19: 229-233 (1985). Thomas, D.G., Breslow, N., and Gart, J. J., "Trend and homogeneity analyses of proportions and life table data," Computers and Biomedical Research, 10:373-381 (1977). 62 Covance 6329-183 3M T-6295 63 Text Table 1 Results of Statistical Analyses of Survival Data for Male Rats Group Unadjusted Mortality (Rate) Adjusted Mortality: Kaplan-Meier Estimate (Final) Standard Error 1 Control 39/70 .557 .778 .059 2 Low 40/60 .667 .800 .057 3 Mid 33/60 .550 .660 .067 4 Mid-High 25/60 .417 5 High 29/70 .414 .500 .565 .071 .070 Covance 6329-183 3M T-6295 6 High Recovery 30/40 .750 .750 .068 Trend (Groups 1 - 5) p (one-sided) Groups 1 vs 2 p (two-sided) Groups 1 vs 3 p (two-sided) Groups 1 vs 4 p (two-sided) Groups 1 vs 5 p (two-sided) Groups 1 vs 6 p (two-sided) Groups 5 vs 6 p (two-sided) Cox-Tarone Test .0005 -** .9849 .1820 .0115 -* .0292 -* .7372 .0980 + 64 Text Table 2 Results of Statistical Analyses of Survival Data for Female Rats Group Unadjusted Mortality (Rate) Adjusted Mortality: Kaplan-Meier Estimate (Final) Standard Error 1 Control 26/70 .371 .520 .071 2 Low 35/60 .583 .700 .065 3 Mid 41/60 .683 .820 .054 4 Mid-High 35/60 .583 5 High 25/70 .357 .700 .498 .065 .071 Covance 6329-183 3M T-6295 6 High Recovery 23/40 .575 .575 .078 Trend (Groups 1 - 5) p (one-sided) Groups 1 vs 2 p (two-sided) Groups 1 vs 3 p (two-sided) Groups 1 vs 4 p (two-sided) Groups 1 vs 5 p (two-sided) Groups 1 vs 6 p (two-sided) Groups 5 vs 6 p (two-sided) Cox-Tarone Test .3394 .1700 + .0015 +** .2338 + .8644 .9407 + .6113 + 65 Covance 6329-183 3M T-6295 Text Table 3 Results of Statistical Analyses of Nonneoplastic Lesions in the Males 12 Group Control Low Liver - Infiltrate, Lymphohistiocytic 51/65 43/55 p-values .1836 - .5720 - 3 Mid 44/55 .5090 + 4 Mid-High 45/55 .4111 + 5 High 45/65 .1592 - 6 High Recovery Groups 5 vs. 6 31/40 NA .2450 + Liver - Hypertrophy, Hepatocellular Centrilobular 0/65 2/55 4/55 .0000 +** .2080 + .0415 +* 22/55 42/65 3/40 .0000 +** .0000 +** .0527 + .0000 -** Liver - Granular Cytoplasm, Eosinophilic, Centrilobular 0/65 0/55 0/55 0/55 .0000 +** NA NA NA 14/65 0/40 .0000 +** NA .0007 -** Liver - Pigment Hepatocellular, Centrilobular 0/65 0/55 0/55 0/55 6/65 0/40 .0006 +** NA NA NA .0139+* NA .0513 - Liver - Necrosis, Individual Hepatocyte 5/65 4/55 6/55 5/55 14/65 4/40 .0106 +* NA NA NA .0224 +* .4699 + .1024 - Liver - Vacuolation, Hepatocellular Midzonal / Centrilobular 3/65 3/55 6/55 13/55 .0000 +** NA .1696 + .0024 +** 19/65 .0001 +** 3/40 .4152 + .0060 -** 66 Covance 6329-183 3M T-6295 Text Table 3 (Continued) Results of Statistical Analyses of Nonneoplastic Lesions in the Males 12 3 4 Group Control Low Mid Mid-High Liver - Focus, Altered Hepatocellular, Clear / Eosinophilic Cell 20/65 21/55 23/55 24/55 p-values .1948 + NA .1431 + .1026 + 5 High 24/65 .2892+ 6 High Recovery Groups 5 vs. 6 16/40 .2243 + .4553 + Liver - Focus, Altered Hepatocellular, Basophilic 10/65 7/55 16/55 p-values .0663 + .4414 - .0556 + 17/55 .0352 +* 13/65 .3232+ 8/40 .3616+ NA Liver - Degeneration, Cystic 5/65 15/55 p-values .0007 +** .0041 +** 19/55 .0003 +** 17/55 .0011 +** 22/65 .0002 +** 15/40 .0002 +** .4306 + Liver - Necrosis, Coagulative 1/65 4/55 p-values .2563 + .1346 + 1/55 NA 6/55 .0351 +* 2/65 NA 4/40 .0683 + .1471 + Liver - Hematopoiesis, Extramedullary 4/65 3/55 8/55 p-values .2161 + NA .1111 + 3/55 NA 7/65 .2652+ 5/40 .2187+ NA Liver - Hyperplasia, Bile Duct 20/65 20/55 p-values .1351 + NA 25/55 .0713 + 24/55 .1026 + 25/65 .2305 + 21/40 .0224 +* .1141 + 67 Covance 6329-183 3M T-6295 Text Table 3 (Continued) Results of Statistical Analyses of Nonneoplastic Lesions in the Males 12 Group Control Low Liver - Fibrosis, Peribiliary 8/65 7/55 p-values .1057 + NA 3 Mid 11/55 .1842 + 4 Mid-High 7/55 NA 5 High 14/65 .1209 + 6 High Recovery Groups 5 vs. 6 4/40 .4901 - .1024 - Liver - Hypertrophy, Hepatocellular Periportal 4/65 1/55 7/55 p-values .3303 - .2387 - .1773 + 2/55 .4222 - 2/65 .3400 - 5/40 .2187+ .0719+ Liver - Infiltrate, Macrophage, Pigmented 6/65 5/55 3/55 p-values .3554 - NA .3357 - 4/55 .4813 - 5/65 NA 2/40 .3491 - .4588 - Liver - Vacuolation, Hepatocellular 6/65 2/55 p-values .4677 + .1976 - 3/55 .3357 - 1/55 .0881 - 7/65 NA 3/40 NA .4254 - Liver - Degeneration / Necrosis Centrilobular 1/65 0/55 0/55 1/55 5/65 1/40 p-values .0132+* NA NA NA .1039+ NA .2566 - Liver - Glycogen, Hepatocellular, Increased 1/65 3/55 0/55 0/55 1/65 0/40 p-values .2206 - .2491 + NA NA NA NA NA 68 Covance 6329-183 3M T-6295 Text Table 3 (Continued) Results of Statistical Analyses of Nonneoplastic Lesions in the Males 12 3 4 5 6 Group Control Low Mid Mid-High High High Recovery Groups 5 vs. 6 Liver - Angiectasis 1/65 1/55 2/55 0/55 2/65 0/40 p-values NA NA NA NA NA NA .3810 - Liver - Lipidosis Subcapsular 0/65 0/55 3/55 1/55 0/65 0/40 p-values .4317 + NA .0934+ NA NA NA NA Pancreas - Zymogen Granules, Decreased 11/65 15/54 11/55 p-values .2162 + .1145 + NA 14/55 .1785 + 16/65 .1937+ 8/40 .4406 + .3831 - Pancreas - Infiltrate, Lymphohistiocytic 2/65 2/54 1/55 p-values .4593 - NA NA 4/55 .2641 + 1/65 NA 1/40 NA NA Pancreas - Infiltrate, Macrophage, Pigmented 24/65 17/54 18/55 p-values .1172- .3350 - .3873 - 13/55 .0845 - 19/65 .2281 - 13/40 .4031 - .4437 + Pancreas - Atrophy, Acinar 7/65 7/54 8/55 9/55 7/65 3/40 p-values .4354 + NA NA .2642+ NA .4254 - .4254 - 69 Covance 6329-183 3M T-6295 Text Table 3 (Continued) Results of Statistical Analyses of Nonneoplastic Lesions in the Males 12 3 Group Control Low Mid Pancreas - Hypertrophy / Basophilia, Acinar Cells 2/65 3/54 0/55 p-values .4568 - NA .2913 - 4 Mid-High 1/55 NA 5 High 3/65 NA 6 High Recovery Groups 5 vs. 6 3/40 .2812+ .4152+ Pancreas - Inflammation, Vascular 2/65 1/54 p-values .4632 + NA 4/55 .2641 + 4/55 .2641 + 1/65 NA 4/40 .1471 + .0683 + Pancreas - Infiltration, Fat, Interstitial 11/65 16/54 p-values .4908 - .0768 + 20/55 .0133 +* 14/55 .1785 + 12/65 NA 6/40 NA .4302 - Pancreas - Thrombus 1/65 0/54 0/55 0/55 0/65 2/40 p-values NA NA NA NA NA .5112 - .1429 + Thyroid - Hyperplasia, C-Cell 9/60 8/49 p-values .4920 - NA 10/50 NA 7/49 .5687 - 9/59 NA 4/39 .3586 - .3467 - Thyroid - Dilatation / Cyst, Follicular 6/60 3/49 p-values .0966 + .3558 - 3/50 .3440 - 9/49 .1631 + 8/59 .3756 + 2/39 .3196 - .1568 - 70 Covance 6329-183 3M T-6295 Text Table 4 Results of Statistical Analyses of Nonneoplastic Lesions in the Females 12 Group Control Low Liver - Infiltrate, Lymphohistiocytic 42/65 42/55 p-values .0080 +** NA 3 Mid 38/55 .3738 + 4 Mid-High 41/55 NA 5 High 56/65 .0038 +** 6 High Recovery Groups 5 vs. 6 32/40 .0709 + .2852 - Liver - Hypertrophy, Hepatocellular, Centrilobular 2/65 1/55 4/55 .0000 +** NA .2641 + 16/55 52/65 2/40 .0001 +** .0000 +** NA .0000 -** Liver - Granular Cytoplasm, Eosinophilic, Centrilobular 0/65 0/55 0/55 7/55 .0000 +** NA NA .0034 +** 36/65 .0000 +** 1/40 .3810+ .0000 -** Liver - Pigment, Hepatocellular, Centrilobular 0/65 0/55 0/55 1/55 36/65 0/40 .0000 +** NA NA NA .0000 +** NA .0000 -** Liver - Necrosis, Individual Hepatocyte 7/65 6/55 6/55 6/55 15/65 3/40 .0359+* NA NA NA .0500 +* .4254 - .0329 -* Liver - Vacuolation, Hepatocellular, Midzonal / Centrilobular 1/65 1/55 0/55 1/55 .0753 + NA NA NA 4/65 .1826 + 1/40 NA .3660 - 71 Covance 6329-183 3M T-6295 Text Table 4 (Continued) Results of Statistical Analyses of Nonneoplastic Lesions in the Females 12 3 Group Control Low Mid Liver - Vacuolation, Hepatocellular, Periportal 15/65 15/55 22/55 p-values .1052 + NA .0358 +* 4 Mid-High 16/55 NA 5 High 22/65 .1217 + 6 High Recovery Groups 5 vs. 6 11/40 .3877 + .3235 - Liver - Focus, Altered Hepatocellular, Clear / Eosinophilic Cell 27/65 21/55 18/55 17/55 p-values .0985 - .4263 - .2109 - .1553 - 21/65 .1818 - 13/40 .2367 - NA Liver - Focus, Altered Hepatocellular, Basophilic 16/65 20/55 13/55 p-values .2314 + .1153 + .5367 - 21/55 .0801 + 20/65 .2785 + 11/40 .4573 + .4487 - Liver - Degeneration, Cystic 0/65 1/55 1/55 2/55 4/65 1/40 p-values .0187 +* NA NA .2080 + .0596 + .3810+ .3660 - Liver - Necrosis, Coagulative 3/65 2/55 4/55 2/55 2/65 2/40 p-values .3857 - NA NA NA NA NA NA Liver - Hematopoiesis, Extramedullary 10/65 17/55 15/55 p-values .0721 - .0352 +* .0851 + 8/55 .5528 - 7/65 .3020 - 5/40 .4579 - NA 72 Covance 6329-183 3M T-6295 Text Table 4 (Continued) Results of Statistical Analyses of Nonneoplastic Lesions in the Females 12 Group Control Low Liver - Hyperplasia, Bile Duct 23/65 25/55 p-values .3266 + .1749 + 3 Mid 19/55 .5389 - 4 Mid-High 17/55 .3738 - 5 High 30/65 .1421 + 6 High Recovery Groups 5 vs. 6 17/40 .2999 + .4358 - Liver - Fibrosis, Peribiliary 9/65 13/55 p-values .4341 - .1264 + 4/55 .1960 - 9/55 NA 10/65 NA 4/40 .3987 - .3168 - Liver - Hypertrophy, Hepatocellular, Periportal 12/65 10/55 9/55 p-values .0026 -** .5796 - .4778 - 4/55 .0614 - 3/65 .0127 -* 7/40 NA .0344 +* Liver - Infiltrate, Macrophage, Pigmented 2/65 3/55 5/55 p-values .0000 +** NA .1567+ 6/55 .0889 + 23/65 7/40 .0000 +** .0147 +* .0383 -* Liver - Vacuolation, Hepatocellular 3/65 2/55 0/55 3/55 3/65 2/40 p-values .4582 + NA .1555 - NA NA NA NA Liver - Cyst, Biliary 3/65 p-values .3708 + 1/55 .3749 - 1/55 .3749 - 1/55 .3749 - 4/65 NA 2/40 NA NA 73 Covance 6329-183 3M T-6295 Text Table 4 (Continued) Results of Statistical Analyses of Nonneoplastic Lesions in the Females 12 Group Control Low Liver - Inflammation, Subacute 2/65 0/55 p-values .2760 - .2913 - 3 Mid 0/55 .2913 - 4 Mid-High 0/55 .2913 - 5 High 1/65 NA 6 High Recovery Groups 5 vs. 6 0/40 .3810 - NA Liver - Angiectasis 6/65 p-values .0231 -* 1/55 .0881 - 2/55 .1976 - 1/55 .0881 - 1/65 .0574 - 2/40 .3491 - .3232 + Liver - Congestion 2/65 2/55 1/55 0/55 0/65 0/40 p-values .0451 -* NA NA .2913 - .2481 - .3810 - NA Pancreas - Zymogen Granules, Decreased 3/65 11/55 14/55 p-values .0488 +* .0093 +** .0011 +** 9/55 .0329 +* 12/65 .0127 +* 10/40 .0030 +** .2879 + Pancreas - Infiltrate, Lymphohistiocytic 3/65 0/55 2/55 3/55 3/65 1/40 p-values .2999 + .1555 - NA NA NA .5071 - .5071 - Pancreas - Hyperplasia, Islet Cells 2/65 1/55 p-values .0372 -* NA 0/55 .2913 - 0/55 .2913 - 0/65 .2481 - 0/40 .3810 - NA 74 Covance 6329-183 3M T-6295 Text Table 4 (Continued) Results of Statistical Analyses of Nonneoplastic Lesions in the Females 12 Group Control Low Pancreas - Atrophy, Acinar 2/65 3/55 p-values .3303 + NA 3 Mid 5/55 .1567 + 45 Mid-High High 2/55 4/65 NA .3400 + 6 High Recovery Groups 5 vs. 6 3/40 .2812+ NA Pancreas - Hypertrophy / Basophilia, Acinar Cells 1/65 5/55 2/55 p-values .1017- .0698 + NA 1/55 NA 0/65 1/40 NA NA .3810+ Pancreas - Infiltration, Fat, Interstitial 10/65 7/55 p-values .2520 - .4414 - 4/55 .1367 - 9/55 NA 6/65 .2120 - 7/40 .4888 + .1721 + Thyroid - Hyperplasia, C-Cell 20/60 19/50 p-values .3153 - NA 19/50 NA 15/50 .4342 - 19/60 NA 16/40 .3190 + .2598 + 75 Covance 6329-183 3M T-6295 Text Table 5 Results of Statistical Analyses of Neoplastic Lesions - Male Rats 12 34 5 Group Control Low Mid Mid-High High Brain - Astrocytoma, Malignant Fatal Incidence 1 0 Incidental Incidence 1 0 Total Incidence Rate 2/60 0/40 0/40 1/30 0/60 6 High Recovery Groups 5 vs 6 0/34 One-sided p-value .3955 - Liver - Hepatocellular, Adenoma Fatal Incidence 0 0 1 0 0 0 Incidental Incidence 0 3 2 1 7 0 Total Incidence Rate 0/60 3/50 3/50 1/50 7/60 0/40 One-sided p-value .0276 +* .1345 + .0689 + NA .0456 +*E NA .0240 -* Pancreas - Islet Cell, Adenoma Fatal Incidence 0 0 0 0 0 0 Incidental Incidence 4 3 4 4 4 1 Total Incidence Rate 4/60 3/49 4/50 4/50 4/60 1/40 One-sided p-value NA NA NA NA NA .2824 - .3021 - 76 Covance 6329-183 3M T-6295 Text Table 5 (Continued) Results of Statistical Analyses of Neoplastic Lesions - Male Rats 12 34 56 Group Control Low Mid Mid-High High High Recovery Groups 5 vs 6 Pancreas - Islet Cell, Carcinoma Fatal Incidence 0 10 0 00 Incidental Incidence 1 1 2 5 53 Total Incidence Rate 1/60 2/49 2/50 5/50 5/60 3/40 One-sided p-value .0681 + NA NA .2487 + .2435 + .2834+ .4972 - Pancreas - Islet Cell, Adenoma/Carcinoma Fatal Incidence 0 1 Incidental Incidence 5 4 Total Incidence Rate 5/60 5/49 0 6 6/50 0 8 8/50 00 94 9/60 4/40 One-sided p-value .1341 + NA NA .2904+ .2027 + NA .3709 - Adrenal Cortex - Adenoma Fatal Incidence 0 Incidental Incidence 3 Total Incidence Rate 3/60 0/42 1/33 2/26 0 1 1/60 0/30 One-sided p-value .2238 - 77 Covance 6329-183 3M T-6295 Text Table 5 (Continued) Results of Statistical Analyses of Neoplastic Lesions - Male Rats 12 3 Group Control Low Mid Adrenal Medulla - Pheochromocytoma, Benign Fatal Incidence 0 Incidental Incidence 3 Total Incidence Rate 3/60 6/42 5/33 45 Mid-High High 0 5 3/26 5/60 6 High Recovery Groups 5 vs 6 1/30 One-sided p-value .3519 + Adrenal Medulla - Pheochromocytoma, Malignant Fatal Incidence 0 Incidental Incidence 1 Total Incidence Rate 1/60 2/42 0/33 0/26 Note: Incidences across groups do not meet selection criterion. 0 1 1/60 0/30 Adrenal Medulla - Pheochromocytoma, Benign/Malignant Fatal Incidence 0 Incidental Incidence 3 Total Incidence Rate 3/60 0 6 6/60 1/30 One-sided p-value .4033 + 78 Covance 6329-183 3M T-6295 Text Table 5 (Continued) Results of Statistical Analyses of Neoplastic Lesions - Male Rats 12 34 Group Control Low Mid Mid-High Testis - Interstitial Cell Tumor, Benign Fatal Incidence 0 0 0 0 Incidental Incidence 1 1 2 2 Total Incidence Rate 1/60 1/50 2/50 2/50 Note: Incidences across groups do not meet selection criterion. 5 High 0 1 1/60 6 High Recovery Groups 5 vs 6 0 1 1/39 Testis - Interstitial Cell Tumor, Malignant Fatal Incidence 0 0 0 0 Incidental Incidence 0 0 0 1 Total Incidence Rate 0/60 0/50 0/50 1/50 Note: Incidences across groups do not meet selection criterion. 00 00 0/60 0/39 Testis - Interstitial Cell Tumor, Benign/Malignant Fatal Incidence 0 0 0 Incidental Incidence 1 1 2 Total Incidence Rate 1/60 1/50 2/50 0 3 3/50 00 11 1/60 1/39 One-sided p-value .4583 + NA NA .3760 + NA NA NA 79 Covance 6329-183 3M T-6295 Text Table 5 (Continued) Results of Statistical Analyses of Neoplastic Lesions - Male Rats 12 34 56 Group Control Low Mid Mid-High High High Recovery Groups 5 vs 6 Thyroid - C-Cell, Adenoma Fatal Incidence 0 0 0 0 00 Incidental Incidence 5 6 6 9 74 Total Incidence Rate 5/60 6/49 6/50 9/49 7/59 4/39 One-sided p-value .3844 + NA NA .2803 + .3745 + NA .3752 - Thyroid - C-Cell, Carcinoma Fatal Incidence 0 Incidental Incidence 1 Total Incidence Rate 0/60 0/49 1/50 0/49 Note: Incidences across groups do not meet selection criterion. 00 01 0/59 1/39 Thyroid - C-Cell, Adenoma/Carcinoma Fatal Incidence 0 0 0 0 Incidental Incidence 5 6 7 9 Total Incidence Rate 5/60 6/49 7/50 9/49 00 75 7/59 5/39 One-sided p-value .4037 + NA .3945 + .2803 + .3745 + .4858 + .4952 + 80 Covance 6329-183 3M T-6295 Text Table 5 (Continued) Results of Statistical Analyses of Neoplastic Lesions - Male Rats 12 34 56 Group Control Low Mid Mid-High High High Recovery Groups 5 vs 6 Thyroid - Follicular Cell, Adenoma Fatal Incidence 0 0 0 0 00 Incidental Incidence 3 5 4 4 49 Total Incidence Rate 3/60 5/49 4/50 4/49 4/59 9/39 One-sided p-value .4998 - .3967 + NA NA NA .0280 +* .0121 +* Thyroid - Follicular Cell, Carcinoma Fatal Incidence 0 0 0 0 Incidental Incidence 3 1 1 2 Total Incidence Rate 3/60 1/49 1/50 2/49 00 11 1/59 1/39 One-sided p-value .2210 - .3855 - .2448 - NA .1734 - .4280 - NA Thyroid - Follicular Cell, Adenoma/Carcinoma Fatal Incidence 0 0 0 Incidental Incidence 6 6 5 Total Incidence Rate 6/60 6/49 5/50 0 5 5/49 00 5 10 5/59 10/39 One-sided p-value .2520 - NA NA NA NA .0970 + .0125 +* 81 Covance 6329-183 3M T-6295 Text Table 5 (Continued) Results of Statistical Analyses of Neoplastic Lesions - Male Rats 12 3 Group Control Low Mid Muscle Skeletal - Sarcoma Undifferentiated, Malignant Fatal Incidence 1 Incidental Incidence 1 Total Incidence Rate 2/60 0/40 0/33 45 Mid-High High 0 0 0/25 0/60 6 High Recovery Groups 5 vs 6 0/30 One-sided p-value .2136 - Pituitary - Adenoma Fatal Incidence Incidental Incidence Total Incidence Rate 18 19 37/60 21 8 29/45 20 22 42/46 14 17 31/36 12 25 37/59 15 13 28/38 One-sided p-value NA NA NA NA NA .4448 + .1455 + Skin - Keratoacanthoma Total Incidence Rate 1/60 4/43 4/37 6/31 5/60 6/33 One-sided p-value NA NA NA NA .1612 + NA NA Multiple Tumor - Lipoma Total Incidence Rate 0/60 1/43 0/50 0/50 3/60 0/40 One-sided p-value NA NA NA NA .2088 + NA .2149 - 82 Covance 6329-183 3M T-6295 Text Table 5 (Continued) Results of Statistical Analyses of Neoplastic Lesions - Male Rats 12 34 Group Control Low Mid Mid-High Multiple Tumor - Liposarcoma Total Incidence Rate 0/60 0/43 0/50 0/50 Note: Incidences across groups do not meet selection criterion. 5 High 1/2 6 High Recovery Groups 5 vs 6 1/40 Multiple Tumor - Lipoma/Liposarcoma Total Incidence Rate 0/60 1/43 0/50 0/50 4/60 1/40 One-sided p-value NA NA NA NA .1143 + NA .3076 - 83 Covance 6329-183 3M T-6295 Text Table 6 Results of Statistical Analyses of Neoplastic Lesions - Female Rats 1 234 56 Group Control Low Mid Mid-High High High Recovery Groups 5 vs 6 Liver - Hepatocellular, Adenoma Fatal Incidence 0 000 00 Incidental Incidence 0 111 52 Total Incidence Rate 0/60 1/50 1/49 1/50 5/60 2/40 One-sided p-value .0153 +* NA NA NA .0386 +* .2040 + .3050 - Liver - Hepatocellular, Carcinoma Fatal Incidence 0 000 Incidental Incidence 0 000 Total Incidence Rate 0/60 0/50 0/49 0/50 Note: Incidences across groups do not meet selection criterion. 00 10 1/60 0/40 Liver - Hepatocellular, Adenoma/Carcinoma Fatal Incidence 0 0 Incidental Incidence 0 1 Total Incidence Rate 0/60 1/50 0 1 1/49 0 1 1/50 00 62 6/60 2/40 One-sided p-value .0057 +** NA NA NA .0204 +* .2040 + .2114 - 84 Covance 6329-183 3M T-6295 Text Table 6 (Continued) Results of Statistical Analyses of Neoplastic Lesions - Female Rats 12 34 56 Group Control Low Mid Mid-High High High Recovery Groups 5 vs 6 Pancreas - Islet Cell, Adenoma Fatal Incidence 0 0 0 0 00 Incidental Incidence 2 2 1 0 20 Total Incidence Rate 2/60 2/50 1/49 0/50 2/60 0/40 One-sided p-value .3126 - NA NA .3484 -E NA .2387 - .2502 - Pancreas - Islet Cell, Carcinoma Fatal Incidence 1 0 0 0 Incidental Incidence 0 0 1 0 Total Incidence Rate 1/60 0/50 1/49 0/50 Note: Incidences across groups do not meet selection criterion. 10 00 1/60 0/40 Pancreas - Islet Cell, Adenoma/Carcinoma Fatal Incidence 1 0 Incidental Incidence 2 2 Total Incidence Rate 3/60 2/50 0 2 2/49 0 0 0/50 10 20 3/60 0/40 One-sided p-value .3421 - NA NA .0513 - NA .1539 - .1750 - 85 Covance 6329-183 3M T-6295 Text Table 6 (Continued) Results of Statistical Analyses of Neoplastic Lesions - Female Rats 12 34 56 Group Control Low Mid Mid-High High High Recovery Groups 5 vs 6 Thyroid - Follicular Cell, Adenoma Fatal Incidence 0 0 0 0 00 Incidental Incidence 0 0 0 2 11 Total Incidence Rate 0/60 0/50 0/49 2/50 1/60 1/40 One-sided p-value .1002+ NA NA .1054+ NA .2387 - NA Thyroid - Follicular Cell, Carcinoma Fatal Incidence 0 0 0 0 Incidental Incidence 0 0 0 1 Total Incidence Rate 0/60 0/50 0/49 1/50 Note: Incidences across groups do not meet selection criterion. 00 00 0/60 0/40 Thyroid - Follicular Cell, Adenoma/Carcinoma Fatal Incidence 0 0 0 Incidental Incidence 0 0 0 Total Incidence Rate 0/60 0/50 0/49 0 3 3/50 00 11 1/60 1/40 One-sided p-value .0790 + NA NA .0471 +* NA .1539 - NA 86 Covance 6329-183 3M T-6295 Text Table 6 (Continued) Results of Statistical Analyses of Neoplastic Lesions - Female Rats 12 34 56 Group Control Low Mid Mid-High High High Recovery Groups 5 vs 6 Thyroid - C-Cell, Adenoma Fatal Incidence 0 0 0 0 00 Incidental Incidence 12 6 6 8 56 Total Incidence Rate 12/60 6/50 6/49 8/50 5/60 6/40 One-sided p-value .0703 - .1478 - .1705 - .2103 - .0336 -* .2392 - .3548 + Thyroid - C-Cell, Carcinoma Fatal Incidence 0 0 0 0 Incidental Incidence 0 10 0 Total Incidence Rate 0/60 1/50 0/49 0/50 Note: Incidences across groups do not meet selection criterion. 00 01 0/60 1/40 Thyroid - C-Cell, Adenoma/Carcinoma Fatal Incidence 0 0 0 0 Incidental Incidence 12 7 6 8 Total Incidence Rate 12/60 7/50 6/49 8/50 00 57 5/60 7/40 One-sided p-value .0560 - .2284 - .1705 - .2103 - .0336 -* .3380 - .2399 + 87 Covance 6329-183 3M T-6295 Text Table 6 (Continued) Results of Statistical Analyses of Neoplastic Lesions - Female Rats Group Pituitary - Adenoma Fatal Incidence Incidental Incidence Total Incidence Rate 1 Control 18 28 46/60 2 Low 23 18 41/50 3 Mid 32 10 42/46 45 Mid-High High 23 14 37/46 15 25 40/60 6 High Recovery Groups 5 vs 6 16 14 30/34 One-sided p-value NA .2409 + NA NA .1726 - NA NA Skin - Squamous Cell, Papilloma Total Incidence Rate 2/60 0/36 0/40 0/35 0/60 1/25 One-sided p-value NA NA NA NA .2209 - NA NA Skin - Squamous Cell, Carcinoma Total Incidence Rate 1/60 0/36 0/40 0/35 Note: Incidences across groups do not meet selection criterion. 0/60 0/25 Skin - Squamous Cell, Papilloma/Carcinoma Total Incidence Rate 3/60 0/36 0/40 0/35 0/60 1/25 One-sided p-value NA NA NA NA .1140 - NA NA 88 Covance 6329-183 3M T-6295 Text Table 6 (Continued) Results of Statistical Analyses of Neoplastic Lesions - Female Rats 1 Group Control Mammary Female- Fibroadenoma Total Incidence Rate 20/60 2 Low 27/50 3 Mid 19/48 45 Mid-High High 24/50 11/60 6 High Recovery Groups 5 vs 6 15/40 One-sided p-value .0152 -* .0337+* .1125 + .2521 + .0235 -* .4099 - .0573 + Mammary Female- Adenoma Total Incidence Rate 7/60 6/50 5/48 7/50 4/60 4/40 One-sided p-value .2359 - NA .4086 + NA .2315 - NA .5055 + Mammary Female- Fibroadenoma/Adenoma Total Incidence Rate 23/60 30/50 22/48 26/50 15/60 16/40 One-sided p-value .0239 -* .0318+* .0527 + .2753 + .0488 -* NA .1451 + Mammary Female- Carcinoma Total Incidence Rate 11/60 12/50 15/48 11/50 14/60 4/40 One-sided p-value .3569 .3996 + .0515 + NA .3373 + .0606 - .0161 -* 89 Covance 6329-183 3M T-6295 Text Table 6 (Continued) Results of Statistical Analyses of Neoplastic Lesions - Female Rats 12 3 Group Control Low Mid Mammary Female- Fibroadenoma/Adenoma/Carcinoma Total Incidence Rate 29/60 36/50 31/48 45 Mid-High High 29/50 24/60 6 High Recovery Groups 5 vs 6 17/40 One-sided p-value .0482 -* .0474 +* .0066 +** NA .1479 - .0598 - .3482 - 90 Covance 6329-183 3M T-6295 TEXT FIGURE 1 KAPLAN-MEIER PRODUCT LIMIT ESTIMATE CURVES OF SURVIVAL: MALE RATS 0 15 30 45 60 75 90 105 1 0+************************************** + 1 1 ***************** 3 *555555 + 1 1 1 1 1 **********22*22222 + *******05 22 + 4****** 222 .9+ ***1 *** 22 P+ 0*******33** R+ 0*04* * O+ 5*0***1* P+ O .8+ 500 41* 5500***33 R+ 5550 *23 T+ 500**** I+ 550**4**3 O+ 55**12*** N 7+ 05*122**444 + 0 51 2 3 4 S+ 0 51 2 3 4 U+ 0 5112233 444 R+ V 6+ 00*5* 223 4 00***2* 44 I+ 0 *5*2 4 V+ 0 1 *22 44 I+ 00 1 *3*33 4 N+ 00*155* 33*33 G 5+ 011 * 44*3 + 00* * 4 + * *22 44 + ***** 4 + 0** 4+ **2 + ***22 + **122 + 0** 22 + 0*55 3+ * 5 + ***5 + *5 + *55 + ** .2 + 1 + + 0 = Group 1 (Control) + 1 = Group 2 (Low) + 2 = Group 3 (Mid) 1 + 3 = Group 4 (Mid-High) + 4 = Group 5 (High) + 5 = Group 6 (High Recovery) + * = More Than One Data Point + 0+ 0 15 30 45 60 75 90 105 TIME (WEEKS) 91 Covance 6329-183 3M T-6295 TEXT FIGURE 2 KAPLAN-MEIER PRODUCT LIMIT ESTIMATE CURVES OF SURVIVAL: FEMALE RATS 0 15 30 45 60 75 90 105 ++++++++ 1 ^0+****************************00000000000 + 222222222222222********************555 + 1 1 1 * * 1 1 33*******3 + 22**************** + *** 5*55 .9+ 1*4 ***5 P+ ***44*****55 R+ 2 11 4* 0 5 O+ 222* *40 55 P+ O .8+ *1 3**0 5 2* 3**4** R+ ***11*** **4 T+ 22 ** 5444 I+ 222 0**** 4 O+ 2 01*54 N 7+ + 22 0***4 2 0*** S+ 22 *3** U+ 2 *15*4 R+ 2 0****4 V 6+ 2 *034444 I+ 2 ***5 4 V+ 22 0** 4 I+ 2 1*5 44 N+ 22 1******4 G 5+ 2 ** 00** + 2 * 5* + 2222 *3 5 + 22 13 5 + 2 1* 55 4+ 2 *3 + 2 1* + 2 **3 + 2 133 + 222 113 3+ 22 1 * + 22 +2 + 222 + 22 2+ 22 +2 + + 0 = Group 1 (Control) + 1 = Group 2 (Low) 1 + 2 = Group 3 (Mid) + 3 = Group 4 (Mid-High) + 4 = Group 5 (High) + 5 = Group 6 (High Recovery) + * = More Than One Data Point 0+ 0 15 30 45 60 75 90 105 TIME (WEEKS) 92 Covance 6329-183 3M T-6295 COMMENTS ON THE DATA Various models of calculators, computers, and computer programs were used to analyze data in this study. Because different models round off or truncate numbers differently, values in some tables (e.g., means, standard deviations, or individual values) may differ slightly from those in other tables, from individually calculated data, or from statistical analysis data. Neither the integrity nor the interpretation of the data was affected by these differences. The units for the dose levels in the inlife summary tables are ppm. The number of animals listed in the heading of the summary tables for clinical and mass observations reflects the number of animals assigned to each group at the start of the study. The summary tables for clinical observations indicate the number of animals for which a condition was observed without regard to the reversibility, number of incidences/animal, or the length of time the condition persisted. Only observations for each animal other than normal are indicated in the summary clinical observations tables. Each animal with observations recorded as "Normal" throughout the study has the comment "No remarkable observations" indicated in the individual clinical observations tables. The specific details for comments in the individual clinical observations tables that are indicated with a "C" can be found at the end of each group for each sex. The level of severity for some clinical observations was not specified at the time of the observation and is indicated as <UNSPECIFIED> in the summary and individual clinical observation tables. The day of initiation of treatment is "Day 1, Week 1." Body weight data are entered at the start of a study week (e.g., a body weight recorded on Day 1 is considered a Week 1 body weight, a body weight recorded on Day 8 is considered a Week 2 body weight). Body weight change data are calculated from the first day of the study week to the first day of the following study week (e.g., Week 1 values are calculated from Day 1 through 8). Weekly food consumption are calculated from the first day of the study week to the first day of the following study week (e.g., Week 1 values are calculated from Day 1 through 8). 93 Covance 6329-183 3M T-6295 COMMENTS ON THE DATA (Continued) The values for summary and individual test material consumption are reported with more significant figures than is appropriate for the data due to limitations of the data collection and reporting software. The comment "SPILLED" in individual food consumption data tables indicates that food consumption was not recorded due to spillage during the interval. The comment "CNTMD" in individual food consumption data tables indicates that food consumption was not recorded due to contamination of the diet with urine or feces in the food container. The comment "NOT TAKEN" in individual food consumption data tables indicates that the animal died before the end of the food consumption interval. The comment "TECH SPILL" in individual food (water) consumption data tables indicates that food consumption was not recorded due to food (water) spillage caused by technician error. Differences in the population size (N) in the summary tables for clinical and anatomic pathology are explained in the individual data tables or the code sheets. Results of clinical pathology samples collected for animals sacrificed at unscheduled intervals appear in the individual clinical pathology tables for the next scheduled interval. These results are not in the summary tables and are not included in the statistical analyses. Statistical analyses were not done when there were two or fewer values for a parameter. 94 Covance 6329-183 3M T-6295 COMMENTS ON THE DATA (Continued) The calculation for individual test material consumption is: Test material consumption = DietConcentrationxFoodConsumption FoodConsumptionIntervalx[First BodyWeight ofInterval+(DailyBodyWeight GainxDayFactor)] where: Daily Body Weight Gain = Last BodyWeight ofInterval- First BodyWeight ofInterval DayofLast BodyWeight ofInterval- DayofFirst BodyWeight ofInterval and: Day Factor = (FoodConsumptionStart Day-DayofFirst BodyWeight ofInterval)+ FoodConsum2ptionInterval and: Food Consumption Interval = FoodConsumptionEndDay-FoodConsumptionStartDay 95 CODES, ABBREVIATIONS, AND UNITS Covance 6329-183 3M T-6295 General Codes and Abbreviations Codes for Clinical Pathology Abbreviations and Units for Clinical Hematology Abbreviations and Units for Clinical Chemistry Abbreviations and Units for Clinical Urinalysis Abbreviations and Units for Urine Chemistry Codes for Anatomic Pathology Note: The following lists of codes, abbreviations, and units are used by Covance. Some, but not necessarily all, of this information may be needed for this report. 96 Covance 6329-183 3M T-6295 General Codes and Abbreviations WK N Mean; MEAN CAM SD; S.D.; STAND DEV; STANDARD DEV; sd * -; NA P C UNSCHED DISPATCH TBW # CO Week Number of measurements in a group Arithmetic mean Covariate-adjusted mean Standard deviation Group mean is significantly different from the mean of the control group (Group 1) at p < 0.05 No value; not applicable; not present Present Comment found at the end of each group for each sex Unscheduled Observations transferred from the inlife module of the data collection system to the necropsy module for reference during necropsy. Observations are duplicates of the last inlife observations. Terminal body weight Number Clinical observation Animal Death Codes 1 2 3 T D M U Interim sacrifice, Week 4 Interim sacrifice, Week 14 Interim sacrifice, Week 53 Terminal sacrifice Found dead Moribund First postrecovery sacrifice 97 NS QS/QNS NR FS SC SH H SL L SI I NF U DT/DOT DB TJ TE RE EE SE PC PD PI PL PA CO HB PLASMO NO AGG FR UTD NO COAG Covance 6329-183 3M T-6295 Codes for Clinical Pathology GENERAL CODES No sample Quantity not sufficient No repeat (sample volume not sufficient for repeat analysis) Fibrin strands Sample clotted Slightly hemolyzed Hemolyzed Slightly lipemic Lipemic Slightly icteric Icteric Animal not fasted Unscheduled/moribund bleed Animal died on test Died during bleeding Technician judgment to repeat test Technical error (instrument or technician error that results in unacceptable data, e.g., unacceptable instrument output, sample spilled, entry of invalid data) Recording error (recorded incorrect data, e.g., wrong number, spelling error, incorrect date) Entry error (incorrect keyboard entry) Sampling error Platelets clumped Platelets decreased Platelets increased Platelets large Platelets appear adequate Color interferes with test Heinz bodies observed Plasmodium No aggregation Fractious Unable to determine No coagulation 98 Covance 6329-183 3M T-6295 Codes for Clinical Pathology (Continued) RESULTS NOT INCLUDED IN STATISTICAL ANALYSES Hemolyzed clinical chemistry or coagulation samples Samples from animals at unscheduled intervals Prothrombin times (PT) greater than 50 seconds Activated partial thromboplastin times (PTT) greater than 110 seconds Bleed times (BLETIME) greater than 30 minutes CODES FOR BLOOD CELL MORPHOLOGY The following scale was used to measure the degree of anisocytosis (ANISO), poikilocytosis (POIK), polychromasia (POLY), hypochromasia (HYPO), or basophilic stippling (BASTIP) or the presence of Howell-Jolly bodies (HJBODY), toxic neutrophils (TOXNEUT), or atypical lymphocytes (ATYPLYM). Scale 1 2 3 4 Degree Normal for the species Slight Moderate Marked Not applicable Presence Not present Rare Few Moderate Many URINE APPEARANCE A Pale B Straw C Yellow D Dark yellow Color E Amber F Brown G Red H Green I Black P Blue/green Q Blue R Orange Clarity J Clear K Hazy L Cloudy Miscellaneous M Debris O Feces 99 Covance 6329-183 3M T-6295 Codes for Clinical Pathology (Continued) URINE CHEMISTRY MULTISTIX STRIP Urine Glucose + ++ +++ ++++ +++++ Negative 100 mg/dL 250 mg/dL 500 mg/dL 1,000 mg/dL >2,000 mg/dL Urine Ketone + ++ +++ ++++ +++++ Negative 5 mg/dL 15 mg/dL 40 mg/dL 80 mg/dL 160 mg/dL Urine Blood - Negative + Small ++ Moderate +++ Large Urine Urobilinogen + ++ +++ ++++ 0.2 mg/dL 1 mg/dL 2 mg/dL 4 mg/dL 8 mg/dL (1 mg = approximately 1 Ehrlich unit) Urine Bilirubin - Negative + Small ++ Moderate +++ Large URINE SEDIMENT Cells, Crystals, Casts, and Comments A Amorphous urates B Amorphous phosphates C Uric acid D Triple phosphates E Calcium oxalate F Calcium carbonate G Granular casts H Hyaline casts I Cellular casts J Waxy casts K Unknown crystal P Mucous threads Q Sperm R Fecal contamination S Pinworm ova found T Pinworm larvae found U Parasite ova found 0 Not present 1 1 -5 per field 2 6 - 1 0 per field 3 11-20 per field 4 >20 per field Bacteria 0 Not present 1 Few 2 Moderate 3 Many 100 Covance 6329-183 3M T-6295 Abbreviations and Units for Clinical Hematology Test Red blood cell count Hemoglobin Hematocrit Mean corpuscular volume Mean corpuscular hemoglobin Mean corpuscular hemoglobin concentration Platelet count Mean platelet volume Reticulocyte count Absolute reticulocyte count Heinz body count Erythrocyte sedimentation rate Prothrombin time Activated partial thromboplastin time Thrombin time Activated coagulation time Fibrinogen Fibrin/fibrinogen degradation products Platelet aggregation Collagen Adenosine diphosphate Alpha 2-antiplasmin Bleeding time Methemoglobin Plasma hemoglobin Myeloid/erythroid ratio Estimated myeloid/erythroid ratio White blood cell count Differential blood cell count Nucleated red blood cell count Corrected white blood cell count Segmented neutrophil count Band neutrophil count Lymphocyte count Monocyte count Eosinophil count Basophil count Anisocytosis Polychromasia Abbreviation (Units) RBC (E6/UL or X106/|iL) HGB (G/DL) HCT (%) MCV (FL) MCH (PG) MCHC (%) PLT (E3/UL or X103/|iL) MPV (FL) RETIC (%) RETIC (e 3/UL or X103/|iL) HEINZ (%) ESR (MM/HR) PT (SEC) PTT (SEC) TT (SEC) ACT (SEC) FBR (MG/DL) FDP (UG/ML) PAGG/COL (%) PAGG/ADP (%) ANTIPLAS (%) BLE TIME (SEC) METHGB (%) PLA HGB (MG/DL) M/E RATIO EST M/E RATIO WBC (E3/UL or X103/|iL) NRBC (/100 WBC) COR WBC (E3/UL or X103/|iL) N-SEG (E3/UL or X103/|iL) and % N-BAND (E3/UL or X103/|iL) and % LYMPH (E3/UL or X103/|iL) and % MONO (E3/UL or X103/|iL) and % EOSIN (E3/UL or X103/|iL) and % BASO (E3/UL or X103/|iL) and % ANISO (-,1,2,3) POLY (-,1,2,3) 101 Covance 6329-183 3M T-6295 Abbreviations and Units for Clinical Hematology (Continued) Test Poikilocytosis Hypochromasia Howell-Jolly bodies Basophilic stippling Toxic neutrophils Atypical lymphocytes Aqueous white blood cell count (right eye) Aqueous white blood cell count (left eye) Abbreviation (Units) POIK (-,1,2,3) HYPO (-,1,2,3) HJBODY (-,1,2,3,4) BASTIP (-,1,2,3) TOXNEUT (-,1,2,3,4) ATYPLYM (-,1,2,3,4) R EYE (WBC/UL) L EYE (WBC/UL) 102 Covance 6329-183 3M T-6295 Abbreviations and Units for Clinical Chemistry Test Glucose Urea nitrogen Urea Creatinine Total protein Albumin Globulin Albumin/globulin ratio Total bilirubin Direct bilirubin Indirect bilirubin Cholesterol Triglyceride Urea nitrogen/creatinine ratio Total lipids Phospholipids High-density lipoprotein cholesterol Low-density lipoprotein cholesterol Uric acid Aspartate aminotransferase Alanine aminotransferase Alkaline phosphatase Gamma glutamyltransferase Sorbitol dehydrogenase Lactate dehydrogenase Creatine kinase Amylase Lipase Palmitoyl CoA oxidase Calcium Ionized calcium Inorganic phosphorus Sodium Potassium Chloride Magnesium Zinc Strontium Iron Abbreviation (Units) GLU (MG/DL) UN (MG/DL) UREA (MG/DL) CREAT (MG/DL) T PRO (G/DL) ALB (G/DL) GLOB (G/DL) A/G RATIO T BILI (MG/DL) D BILI (MG/DL) I BILI (MG/DL) CHOL (MG/DL) TRIG (MG/DL) UN/CREAT (RATIO) T LIPIDS (MG/DL) P LIPIDS (MG/DL) HDL (MG/DL) LDL (MG/DL) UA (MG/DL) AST/SGOT (IU/L) ALT/SGPT (IU/L) ALK PHOS (IU/L) GGT (IU/L) SDH (IU/l ) LDH (IU/L) CK (IU/L) AMYLASE (IU/L) LIPASE (IU/L) PCOAO (IU/G) CA (MG/DL) ION CA (MG/DL) I PHOS (MG/DL) NA (MMOL/L) K (MMOL/L) CL (MMOL/L) MG (MEQ/L or MG/DL) ZN (MG/L or PPM) SR (MG/L or PPM) FE (UG/DL) 103 Covance 6329-183 3M T-6295 Abbreviations and Units for Clinical Chemistry (Continued) Test Excess iron Total iron binding capacity Unbound iron binding capacity Percent iron saturation Plasma cholinesterase Red blood cell cholinesterase Brain cholinesterase Caudate putamen Hippocampus Frontal cortex Cerebellum Bicarbonate Serum hemoglobin Serum bile acids Fecal bile acids Average fecal weight Fecal bile acids (calculation) Osmolality Electrophoresis Albumin Alpha-1-globulin Alpha-2-globulin Beta globulin Gamma globulin High-density lipoprotein Low-density lipoprotein Very-low-density lipoprotein Insulin Adrenocorticotropic hormone Cortisol Glucagon Triiodothyronine Thyroxine Creatine kinase isoenzymes BB MB MM Abbreviation (Units) EX FE (UG/DL) TIBC (UG/DL) UIBC (UG/DL) FE %SAT (%) CHEP (MU/ML) CHER (MU/ML) CHEB (MU/ML) CAUD PUT (UMOL/G) HIPPOCAM (UMOL/G) F CORTEX (UMOL/G) CEREBELL (UMOL/G) BICARB (MMOL/L) SER HGB (MG/DL) SBA (UMOL/L or MG/DL) FBA (UG/ML) FCC WGT (G) FBA (MG/Day) OSMO (MOSMTKG) E ALB (G/DL) E A-1 (G/DL) E A-2 (G/DL) E BETA (G/DL) E GAMMA (G/DL) E-HDL (%) E-LDL (%) E-VLDL (%) INSULIN (UU/ML) ACTH (PG/ML) CORTISOL (UG/ML) GLUCAGON (PG/ML) T3 (NG/DL) T4 (UG/DL) CK-BB (U/L) CK-MB (U/L) CK-MM (U/L) 104 Covance 6329-183 3M T-6295 Abbreviations and Units for Clinical Urinalysis Test Urine volume 8-hour urine volume Specific gravity Urine osmolality Quantitative urinary/cerebrospinal fluid protein Urine protein excretion Urine chemistry Multistix strip Urine pH Urine protein Urine glucose Urine ketones Urine bilirubin Urine blood Urine urobilinogen Urine reducing substances Microscopic examination of urine sediment Red blood cells per high-power field White blood cells per high-power field Epithelial cells per high-power field Bacteria per high-power field Casts per low-power field Crystals per low-power field Urine appearance Comments Abbreviation (Units) U VOL (ML) 8 HR VOL (ML) SPGR U OSMO (MOSM/KG) QUAN PRO (MG/DL) PRO EXC (MG) UPH U PRO (MG/DL) U GLU UKET UBILI U BLOOD UROBILI U RE SUB RBC (PER HPF) WBC (PER HPF) EPITH (PER HPF) BACT (PER HPF) CASTS (PER LPF) CRYSTALS (PERLPF1 or PER LPF2) URINE APPI or URINE APP2 COMMENTS 105 Covance 6329-183 3M T-6295 Abbreviations and Units for Urine Chemistry Test Urine volume Urine osmolality Urine creatinine Urine calcium Urine calcium/creatinine ratio Urine phosphorus Urine sodium Urine potassium Urine chloride Urine magnesium Urine zinc Urine strontium Urine hydroxyproline N-ACETYL-P-D-glucosaminedase Creatinine excretion Calcium excretion Phosphorus excretion Sodium excretion Potassium excretion Chloride excretion Calcium fractional clearance Phosphorus fractional clearance Sodium fractional clearance Potassium fractional clearance Chloride fractional clearance Creatinine clearance Quantitative urinary/cerebrospinal fluid protein Protein to urinary creatinine ratio Urine protein excretion Abbreviation (Units) U VOL (ML) U OSMO (MOSM/KG) U CREAT (MG/DL) U CA (MG/DL) CA/CREAT (RATIO) U PHOS (MG/DL) U NA (MMOL/L) U K (MMOL/L) U CL (MMOL/L) U MG (MG/L or PPM) U ZN (MG/L or PPM) U SR (MG/L or PPM) U HYP (MG/L) NAG (U/L) CREA EXC (MG) CA EXC (MG) P EXC (MG) NA EXC (MMOL) K EXC (MMOL) CL EXC (MMOL) CA CLEAR (%) P CLEAR (%) NA CLEAR (%) K CLEAR (%) CL CLEAR (%) CREAT CL (ML/MIN) QUAN PRO (MG/DL) PRO/CREA (RATIO) PRO EXC (MG) 106 Code 1-6 T U V A B D M P R O Codes for Anatomic Pathology Definition ANIMAL DEATH CODES Interim sacrifices 1 through 6 Terminal sacrifice First postrecovery sacrifice Second postrecovery sacrifice Accidental death Death or sacrifice postaccident Found dead Moribund Returned to stock Unscheduled recovery sacrifice Other type of unscheduled death Covance 6329-183 3M T-6295 EX NOT TAKEN MISSING UNSUITABLE AUTOLYTIC EXCLUDE MACROSCOPIC CODES Indicates that organ weight is excluded from calculations Organ weight not taken; explanation given in necropsy notes Organ missing or lost Organ technically unsuitable for weighing Organ autolyzed and could not be weighed Weight was taken, but was excluded from all calculations MICROSCOPIC CODES Codes Prefacing Neoplastic Findings B- Primary, benign neoplasm M- Primary, malignant neoplasm N- Metastatic neoplasm I- Locally invasive neoplasm X- Other neoplasm Distribution of Findings Focal Diffuse Multifocal 107 Covance 6329-183 3M T-6295 Codes for Anatomic Pathology (Continued) Grades for Severity or Amount 1 Minimal - the least amount of change that can be observed with the light microscope 2 Slight - less than average amount of change, but readily discernible as abnormal 3 Moderate - the average amount of change that is expected for a lesion 4 Moderately severe (marked) - a marked amount of change with possible loss of function of the affected cells or organs 5 Severe - a great amount of change with probable loss of function of the affected cell or organs and frequently involves large areas of the organ Other Microscopic Codes TL Total P Finding present - Finding not present MN Mean TISSUE ABBREVIATIONS Abbreviation LN GL STOMACH, GL STOMACH, NONGL SALIV GL, MANDIB LN, ANT MES/PANC AUDITORY SEB GL LACRIMAL GLAND, EX HEMATO NEOPLASIA LACRIMAL GL, INT CAVITY, ABDOM SALIV GL,PAROTID LN, TRACHEOBRON CATHETER EXIT CATHETER ENTRANC CATHETER TIP Definition Lymph node Gland Glandular stomach Nonglandular stomach Mandibular salivary gland Anterior mesenteric/pancreatic lymph node Auditory sebaceous gland Exorbital lacrimal gland Hematopoietic neoplasia Internal lacrimal gland Abdominal cavity Parotid salivary gland Tracheobronchial lymph node Catheterization site: exit site from the body Catheterization site: entrance site into the vessel Catheterization site: tissues (vascular or extravascular) associated with the catheter near its tip 108 Figure 1 Survival Data - Males Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS 109 100 90 80 70 60 50 40 30 20 10 0 17 Figure 2 Survival Data - Females 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS Covance 6329-183 3M T-6295 4 Group 1 - 0 ppm 100 Group 2 - 0.5 ppm 100 Group 3 - 2 ppm 100 Group 4 - 5 ppm 100 Group 5 - 20 ppm 100 Group 6 - 20 ppm 100 uI 13 19 25 31 37 43 49 55 61 67 73 79 85 91 97 103 Week % 110 Figure 3 Mean Body Weight Data (g) - Males Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS 900 800 700 600 500 400 Group 1 0 ppm Group 2 0.5 ppm Group 3 2 ppm 300 Group 4 5 ppm Group 5 20 ppm 200 Group 6 20 ppm 100 0 T-----------------T 1 7 13 19 25 31 37 43 49 55 61 67 73 79 85 91 97 103 Week (g) 111 Figure 4 Mean Body Weight Data (g) - Females 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS Covance 6329-183 3MT-6295 112 215 -, Figure 5 Mean Food Consumption Data (g) - Males 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS Covance 6329-183 3M T-6295 (g) 135 Group - 0 ppm Group 2 - 0.5 ppm Group 3 - 2 ppm Group 4 - 5 ppm Group 5 - 20 ppm 115 Group 6 - 20 ppm 95 / O 1 ---------- ----------- 1----------------------1----------- -----------1----------------------1----------------------1---------- ----------- 1----------------------1----------------------1----------------------1----------------------1---------- ----------- 1----------------------1----------------------1----------------------1----------------------1---------- ----------- 1---------- ------------1-- 1 7 13 19 25 31 37 43 49 55 61 67 73 79 85 91 97 103 Week 113 Figure 6 Mean Food Consumption Data (g) - Females 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS Covance 6329-183 3M T-6295 (g) 114 Table 1 Covance 6329-183 3M T-6295 Results of Homogeneity Analyses (ppm) Mixed 3/18/98 104-Week Dietary Chronic Toxicity And Carcinogenicity Study With Perfluorooctane Sulfonic Acid Potassium Salt (Pfos; T-6295) In Rats Samples Location Replicate 0.5 T-6295 (ppm) 20 Top 1 0.605 21.3 2 0.588 21.4 Mean 0.597 (119)a 21.4 (107) Middle Bottom 1 2 Mean 1 2 Mean 0.657 0.690 0.674 (135) 0.601 0.631 0.616 (123) 23.6 23.5 23.6 (118) 24.6 20.4 22.5 (113) a Each value in parentheses is the percent of theoretical. 115 Table 1 (Continued) Covance 6329-183 3M T-6295 Results of Homogeneity Analyses (ppm) Mixed 4/16/98 104-Week Dietary Chronic Toxicity And Carcinogenicity Study With Perfluorooctane Sulfonic Acid Potassium Salt (Pfos; T-6295) In Rats Sample Location Replicate 0.5 T-6295 (ppm) 25 20 Top 1 0.743 2.22 5.36 18.1 2 0.521 2.03 5.48 17.4 3 0.406 2.12 4.04 18.4 Mean 0.557 (111)a 2.12 (106) 4.96 (99.2) 18.0 (90.0) Middle Bottom 1 2 3 Mean 1 2 3 Mean 0.478 0.478 0.486 0.481 (96.2) 2.28 2.01 2.09 2.13 (107) 4.90 4.90 5.28 5.03 (101) 19.7 19.5 21.3 20.2 (101) 0.955 0.519 0.359 0.611 (122) 2.04 2.03 1.84 1.97 (98.5) 4.70 5.00 4.94 4.88 (97.6) 17.6 18.7 18.4 18.2 (91.0) a Each value in parentheses is the percent of theoretical. 116 Table 1 (Continued) Covance 6329-183 3M T-6295 Results of Homogeneity Analyses (ppm) Mixed 04/22/98 104-Week Dietary Chronic Toxicity And Carcinogenicity Study With Perfluorooctane Sulfonic Acid Potassium Salt (Pfos; T-6295) In Rats Sample Location Replicate T-6295 (ppm) 20 Top 1 18.9 2 18.4 Mean 18.7 (93.5)a Middle Bottom 1 2 Mean 1 2 Mean 20.1 19.6 19.9 (99.5) 21.1 20.1 20.6 (103) a Each value in parentheses is the percent of theoretical. 117 Covance 6329-183 3M T-6295 Table 1 (Continued) Results of Homogeneity Analyses (ppm) Mixed 6/18/98 104-Week Dietary Chronic Toxicity And Carcinogenicity Study With Perfluorooctane Sulfonic Acid Potassium Salt (Pfos; T-6295) In Rats _________________ T-6295 (ppm)_________ Sample Location Replicate 0.5 2 5 20 Top Topb Middle Bottom 1 2 3 Mean 1 2 3 Mean 1 2 3 Mean 1 2 3 Mean 0.566 1.10 0.955 0.874 (175) 2.38 2.71 2.23 2.44 (122) 4.87 5.18 4.90 4.98 (99.6) 20.3 18.7 18.6 19.2 (96.0) 0.483 0.480 0.401 0.455 (91.0) - - - 0.617 0.637 0.635 0.630 (126) 2.50 2.11 2.05 2.22 (111) 5.15 5.43 4.70 5.09 (102) 18.3 17.8 18.2 18.1 (90.5) 0.640 0.694 0.532 0.622 (124) 3.08 2.29 2.41 2.59 (130) 5.08 5.12 5.15 5.12 (102) 19.4 20.5 18.2 19.4 (97.0) a Each value in parentheses is the percent of theoretical. b Reassay. 118 Covance 6329-183 3M T-6295 Table 2 Results of Stability Analyses (ppm) Mixed 3/18/98 104-Week Dietary Chronic Toxicity And Carcinogenicity Study With Perfluorooctane Sulfonic Acid Potassium Salt (Pfos; T-6295) In Rats Storage Conditions Replicate 0.5 T6295 (ppm) 1.0 2.0 20 Initiala 1 2 Mean 0.657 1.15 2.29 23.6 0.690 1.51 2.67 23.5 0.674 (135)b 1.33 (133) 2.48 (124) 23.6 (118) 28 days, room temperature 1 2 Mean 0.507 1.11 1.94 20.7 0.554 0.937 2.01 20.8 0.531 (106) 1.02 (102) 1.98 (99.0) 20.8 (104) a Results for the 0.5- and 20- levels were derived from the sample collected from the middle of the dose preparations for homogeneity analysis. b Each value in the parenthesis is the percent of theoretical. 119 Covance 6329-183 3M T-6295 Table 2 (Continued) Results of Stability Analyses (ppm) Mixed 4/16/98 104-Week Dietary Chronic Toxicity And Carcinogenicity Study With Perfluorooctane Sulfonic Acid Potassium Salt (Pfos; T-6295) In Rats Storage Conditions Replicate 0.5 T6295 (ppm) 2.0 5.0 20 Initiala 1 2 3 Mean 0.478 0.478 0.486 0.481 (96.2)b 2.28 2.01 2.09 2.13 (107) 4.90 4.90 5.28 5.03 (101) 19.7 19.5 21.3 20.2 (101) 33 days, room temperature 1 2 Mean 0.527 0.553 0.540 (108) 2.57 5.64 28.4 2.54 5.83 22.1 2.56 (128) 5.74 (115) 25.6 (128) a Results were derived from the sample collected from the middle of the dose preparations for homogeneity analysis b Each value in the parenthesis is the percent of theoretical. 120 Covance 6329-183 3M T-6295 Table 3 Results of Dose Preparation Analysis (ppm) 104-Week Dietary Chronic Toxicity And Carcinogenicity Study With Perfluorooctane Sulfonic Acid Potassium Salt (Pfos; T-6295) In Rats Mix Date Replicate 0.5 T-6295 (ppm) 12 20 3/18/98 1 2 Mean 0.657 0.690 0.674 (135)b 1.15 1.51 1.33 (133) 2.29 23.6 2.67 23.5 2.48 (124) 23.6 (118) a Results for the 0.5- and 20- levels were derived from the sample collected from the middle of the dose preparations for homogeneity analysis. b Each value in the parentheses is the percent of theoretical. 121 Covance 6329-183 3M T-6295 Table 3 (Continued) Results of Dose Preparation Analysis (ppm) 104-Week Dietary Chronic Toxicity And Carcinogenicity Study With Perfluorooctane Sulfonic Acid Potassium Salt (Pfos; T-6295) In Rats Mix Date Replicate 0 0.5 T-6295 (ppm) 25 20 4/16/98a 1 2 3 Mean b b - - 0.478 2.28 0.478 2.01 0.486 2.09 0.481 (96.2)c 2.13 (107) 4.90 4.90 5.28 5.03 (101) 19.7 19.5 21.3 20.2 (101) 4 23 98 4/23/98d 4/23/98e 1 2 Mean 1 2 Mean 1 2 Mean b b - - - 0.375 2.07 6.54 19.9 0.379 1.76 4.92 21.3 0.377 (75.4) 1.92 (96.0) 5.73 (115) 20.6 (103) 0.674 0.535 0.605 (121) - 0.780 4.78 2.78 (55.6) - 0.517 0.406 0.462 (92.4) - 4.55 6.12 5.34 (107) - 4/30/98 4/30/98d 1 2 Mean 1 2 Mean b b - - 0.551 0.478 0.515 (103) - 2.12 2.11 2.12 (106) - 5.55 6.03 5.79 (116) 5.24 5.24 5.24(105) 22.6 22.1 22.4 (112) - a Results for the 0.5-, 2.0-, 5.0- and 20-ppm levels were derived from the sample collected from the middle of the dose preparations for homogeneity analysis. b Below the limit of quantitation (<0.4 ppm). c Each value in the parentheses is the percent of theoretical. d Reassay. e Retention. 122 Covance 6329-183 3M T-6295 Table 3 (Continued) Results of Dose Preparation Analysis (ppm) 104-Week Dietary Chronic Toxicity And Carcinogenicity Study With Perfluorooctane Sulfonic Acid Potassium Salt (Pfos; T-6295) In Rats Mix Date Replicate 0 0.5 T-6295 (ppm) 25 20 5/7/98 1 2 Mean 0.962 0.406 1.73 4.37 18.2 0.942 0.390 1.74 4.31 18.2 0.952 0.398 (79.6)c 1.74 (87.0) 4.34 (86.8) 18.2 (91.0) 5/7/98d 1 2 Mean 1.11 0.202 1.12 0.557 1.12 0.380 (76.0) - - - 5/7/98e 1 2 Mean b b - 0.476 0.347 0.412 (82.4) - - - 5/14/98 1 2 Mean b b - 0.474 0.402 0.438 (87.6) 2.52 2.54 2.53 (127) 5.48 5.06 5.27 (105) 19.8 21.5 20.7 (104) 5/21/98f 1 2 Mean <0.75 0.157 <0.75 0.349 - 0.253 (50.6) 1.60 4.98 1.86 6.27 1.73 (86.5) 5.63 (113) 19.6 20.9 20.3 (102) 5/21/98d 1 2 Mean b b - 0.248 0.326 0.287 (57.4) - - - 5/28/98 1b 0.442 1.95 6.19 2b 0.453 2.29 6.49 Mean - 0.448 (89.6) 2.12 (106) 6.34 (127) b Below the limit of quantitation (<0.4 ppm). c Each value in the parentheses is the percent of theoretical. d Reassay. e Retention. f The low standard (0.4 ppm) was not used for the calibration curve. 21.8 22.3 22.1 (111) 123 Covance 6329-183 3M T-6295 Table 3 (Continued) Results of Dose Preparation Analysis (ppm) 104-Week Dietary Chronic Toxicity And Carcinogenicity Study With Perfluorooctane Sulfonic Acid Potassium Salt (Pfos; T-6295) In Rats Mix Date Replicate 0 0.5 T-6295 (ppm) 25 20 6/4/98g 1 2 Mean b b - 0.618 1.95 4.91 18.2 0.610 1.66 5.39 20.3 0.614 (123)c 1.81 (90.5) 5.15 (103) 19.3 (96.5) 6/11/98g 1 2 Mean b b - 0.446 2.25 0.539 1.91 0.493 (98.6) 2.08 (104) 5.22 19.0 4.81 16.9 5.02 (100) 18.0 (90.0) 6/18/98a 1 2 3 Mean b b - - 0.617 0.637 0.635 0.630 (126) 2.50 2.11 2.05 2.22 (111) 5.15 5.43 4.70 5.09 (102) 18.3 17.8 18.2 18.1 (90.5) 7/16/98 7/16/98d 1 2 Mean 1 2 Mean b b - - 0.564 0.519 0.542 (108) 1.60 3.90 17.9 1.90 4.13 17.4 1.75 (87.5) 4.02 (80.4) 17.7 (88.5) - - 4.76 - - 5.41 - - 5.09 (102) - 8/13/98 1 2 Mean b b - 0.478 0.569 0.524 (105) 2.07 2.04 2.06 (103) 5.60 18.9 4.95 19.9 5.28 (106) 19.4 (97.0) a Results for the 0.5-, 2.0-, 5.0- and 20-ppm levels were derived from the sample collected from the middle of the dose preparations for homogeneity analysis. b Below the limit of quantitation (<0.4 ppm). c Each value in the parentheses is the percent of theoretical. d Reassay. g Reassayed due to unacceptable calibration curve for original assays. 124 Covance 6329-183 3M T-6295 Table 3 (Continued) Results of Dose Preparation Analysis (ppm) 104-Week Dietary Chronic Toxicity And Carcinogenicity Study With Perfluorooctane Sulfonic Acid Potassium Salt (Pfos; T-6295) In Rats Mix Date Replicate 0 0.5 T-6295 (ppm) 2.0 5.0 20 9/10/98 9/10/98d 9/10/98e 9/10/98d,e 9/10/98f 1 2 Mean 1 2 Mean 1 2 Mean 1 2 Mean 1 2 Mean b b - - - - - 0.477 2.23 4.58 17.1 2.28 2.27 4.64 16.8 1.38 (276)c 2.25 (113) 4.61 (92.2) 17.0 (85.0) 0.213 0.231 0.222 (44.4) - - - 1.09 1.27 1.18 (236) - - - b -- - b -- - - -- - 0.475 0.386 0.431 (86.2) - - - 10/8/98 1 2 Mean b b - 0.836 0.482 0.659 (132) 1.79 5.68 1.83 4.90 1.81 (90.5) 5.29 (106) 17.9 17.2 17.6 (88.0) b Below the limit of quantitation (<0.4 ppm). c Each value in the parentheses is the percent of theoretical. d Reassay. e Retention. f Reassay of the retention. 125 Covance 6329-183 3M T-6295 Table 3 (Continued) Results of Dose Preparation Analysis (ppm) 104-Week Dietary Chronic Toxicity And Carcinogenicity Study With Perfluorooctane Sulfonic Acid Potassium Salt (Pfos; T-6295) In Rats Mix Date Replicate 0 0.5 T-6295 (ppm) 2.0 5.0 20 10/8/98d 1 b 2 - 0.649 (130)c - - - Mean - - -- - 10/8/98e 1 - 0.473 - - - 2 - 1.02 - - - Mean - 0.747 (149) - - - 10/8/98f 1 - 0.474 - - - 2 - 0.497 - - - Mean - 0.486 (97.2) - - - 11/5/98 12/3/98 12/3/98d 1 2 Mean 1 2 Mean 1 2 Mean b b - b b - - 0.546 0.556 0.551 (110) 1.84 4.33 16.3 2.05 4.45 15.4 1.95 (97.5) 4.39 (87.8) 15.9 (79.5) b 1.65g 3.86 14.6 b 1.51 3.69 16.6 - 1.58 (79.0) 3.78 (75.6) 15.6 (78.0) 0.607 0.539 0.573 (115) 1.98 1.69 1.84 (92.0) - - b Below the limit of quantitation (<0.4 ppm). d Reassay. c Each value in the parentheses is the percent of theoretical. e Retention. f Reassay of the retention g Group will reassayed due to unacceptable chromatography. 126 Covance 6329-183 3M T-6295 Table 3 (Continued) Results of Dose Preparation Analysis (ppm) 104-Week Dietary Chronic Toxicity And Carcinogenicity Study With Perfluorooctane Sulfonic Acid Potassium Salt (Pfos; T-6295) In Rats Mix Date Replicate 0 0.5 T-6295 (ppm) 2.0 5.0 20 12/31/98 12/31/98d 1 2 Mean 1 2 Mean b b - - 0.339 1.89 4.42 19.0 0.246 1.69 4.71 19.4 0.293 (58.6)c 1.79 (89.5) 4.57 (91.4) 19.2 (96.0) 0.442 - - - 0.422 - - - 0.432 (86.4) - - - 1/28/99 1/28/99d 1/28/99d 1 2 Mean 1 2 Mean 1 2 Mean b b - - - e e - b b - b b - 1.67 4.71 17.6 2.05 4.92 17.1 1.86 (93.0) 4.82 (96.4) 17.4 (87.0) - -- -- -- - -- -- -- 2/25/99 2/25/99d 1 2 Mean 1 2 Mean 1.498 0.907 b 0.852 - 0.880 (176) 2.06 6.79 4.43 (222) 5.67 20.1 7.48 20.1 6.58 (132) 20.1 (101) b 0.450 1.64 4.91 - b 0.526 2.06 4.56 - b 0.488 (97.6) 1.85 (92.5) 4.74 (94.8) - b Below the limit of quantitation (<0.4 ppm). c Each value in the parentheses is the percent of theoretical. d Reassay. e This level was outside of the modified standard curve and will be retested. 127 Covance 6329-183 3M T-6295 Table 3 (Continued) Results of Dose Preparation Analysis (ppm) 104-Week Dietary Chronic Toxicity And Carcinogenicity Study With Perfluorooctane Sulfonic Acid Potassium Salt (Pfos; T-6295) In Rats Mix Date Replicate 0 0.5 T-6295 (ppm) 2.0 5.0 20 3/25/99 3/25/99d 1 2 Mean 1 2 Mean b b - - 0.414 (82.8)c 1.91 b 2.97 - 2.44 (122) 5.44 5.59 5.52 (110) 18.5 19.9 19.2 (96.0) 1.63 0.702 1.17 (234) 2.18 1.91 2.05 (103) - - 4/22/99 4/22/99d 1 2 Mean 1 2 Mean b b - - b 1.97 4.73 17.7 b 2.04 5.72 18.8 - 2.01 (101) 5.23 (105) 18.3 (91.5) 0.431 0.405 0.418 (83.6) - 5.06 4.90 4.98 (99.6) - 5/20/99 1 2 Mean b b - 0.582 0.531 0.557 (111) 1.76 4.65 17.8 1.68 4.67 20.1 1.72 (86.0) 4.66 (93.2) 19.0 (95.0) 6/17/99 1 2 Mean b b - 0.402 0.455 0.429 (85.8) 1.73 4.46 19.0 2.03 4.81 18.5 1.88 (94.0) 4.64 (92.8) 18.8 (94.0) b Below the limit of quantitation (<0.4 ppm). c Each value in the parentheses is the percent of theoretical. d Reassay. 128 Covance 6329-183 3M T-6295 Table 3 (Continued) Results of Dose Preparation Analysis (ppm) 104-Week Dietary Chronic Toxicity And Carcinogenicity Study With Perfluorooctane Sulfonic Acid Potassium Salt (Pfos; T-6295) In Rats Mix Date Replicate 0 0.5 T-6295 (ppm) 2.0 5.0 20 7/15/99 7/15/99d 1 2 Mean 1 2 Mean b b - - 0.498 1.75 0.405 23.7 0.452 (90.4)c 12.7 (635) 5.74 4.64 5.19 (104) 18.2 18.6 18.4 (92.0) - 1.77 - - - 1.91 - - - 1.84 (92.0) - - 8/12/99 8/12/99d 1 2 Mean 1 2 Mean b b - - 0.455 0.544 0.500 (100) - 3.15 2.16 2.66 (133) 5.96 22.0 5.80 20.8 5.88 (118) 21.4 (107) 2.13 2.12 2.13 (107) - - 9/9/99f 9/9/99g 1 2 Mean 1 2 Mean e e - b b - e e - 1.27 0.607 0.939 (188) 1.80 2.33 2.07 (104) 4.77 20.5 5.25 21.0 5.01 (100) 20.8 (104) - -- -- -- b Below the limit of quantitation (<0.4 ppm). c Each value in the parentheses is the percent of theoretical. d Reassay. e Samples to be re-analyzed. f Two lowest standards dropped. g Re-test. 129 Covance 6329-183 3M T-6295 Table 3 (Continued) Results of Dose Preparation Analysis (ppm) 104-Week Dietary Chronic Toxicity And Carcinogenicity Study With Perfluorooctane Sulfonic Acid Potassium Salt (Pfos; T-6295) In Rats Mix Date Replicate 0 0.5 T-6295 (ppm) 2.0 5.0 20 9/9/99g 1 0.407 2- 0.406 - - - Mean - 0.407 (81.4)c - - - 10/7/99 1 2 Mean b b - 0.515 0.541 0.528 (106) 2.22 1.98 2.10 (105) 5.90 22.1 5.64 22.9 5.77 (115) 22.5 (113) 11/4/99 1 2 Mean b b - 0.701 0.553 0.627 (125) 1.80 4.31 21.7 1.98 4.23 20.7 1.89 (94.5) 4.27 (85.4) 21.2 (106) 12/2/99 1 2 Mean b b - 0.479 0.487 0.483 (96.6) 2.15 2.28 2.22 (111) 5.93 22.8 5.24 23.8 5.59 (112) 23.3 (117) 12/30/99 12/30/99 g 1 2 Mean 1 2 Mean b b - - 0.453 0.469 0.461 (92.2) 1.74 4.67 h 2.03 4.16 h 1.89 (94.5) 4.42 (88.4) - - - - 23.9 - - - 23.4 - - - 23.7 (119) b Below the limit of quantitation (<0.4 ppm). c Each value in the parentheses is the percent of theoretical. g Re-test. h Due to dropping high standard, this sample couldn't be quantitated. It will be retested. 130 Covance 6329-183 3M T-6295 Table 3 (Continued) Results of Dose Preparation Analysis (ppm) 104-Week Dietary Chronic Toxicity And Carcinogenicity Study With Perfluorooctane Sulfonic Acid Potassium Salt (Pfos; T-6295) In Rats Mix Date Replicate 0 0.5 T-6295 (ppm) 2.0 5.0 20 1/27/00 1 2 Mean b b - 0.455 2.26 0.424 1.87 0.440 (88.0)c 2.07 (104) 5.85 22.2 5.21 21.2 5.53 (111) 21.7 (109) 2/24/00 2/24/00g 3/23/00 1 2 Mean 1 2 Mean 1 2 Mean b b - - - 0.390 0.405 0.398 (79.6) 2.66 2.18 2.42 (121) 8.05 23.3 7.48 26.0 7.77 (155) 24.7 (124) - 2.35 6.18 21.4 - 2.42 5.80 20.0 - 2.39 (120) 5.99 (120) 20.7 (104) 0.240 0.479 0.360 (72.0) 2.38 2.00 2. 19(110) 8.05 25.0 6.32 26.5 7.19 (144) 25.8 (129) Overall mean concentrations of each dose level ranged from 102.3 to 108.7. b Below the limit of quantitation (<0.4 ppm). c Each value in the parentheses is the percent of theoretical. g Re-test Overall Mean = Total of all means Number of means 131 TABLE 4 Survival Data Covance 6329-183 3M T-6295 GROUP AND DOSE LEVEL (PPM) 1 0 2 500 3 2 4 5 5 20 6 20 WEEK: START 70/70 100% 60/60 100% 60/60 100% 60/60 100% 70/70 100% 40/40 100% 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS 1 70/70 100% 60/60 100% 60/60 100% 60/60 100% 70/70 100% 40/40 100% 2345678 70/70 100% 60/60 100% 60/60 100% 60/60 100% 70/70 100% 40/40 100% 70/70 100% 60/60 100% 60/60 100% 60/60 100% 70/70 100% 40/40 100% MALE 65/65 100% 55/55 100% 55/55 100% 55/55 100% 65/65 100% 40/40 100% 65/65 100% 55/55 100% 55/55 100% 55/55 100% 65/65 100% 40/40 100% 65/65 100% 55/55 100% 55/55 100% 55/55 100% 65/65 100% 40/40 100% 65/65 100% 55/55 100% 55/55 100% 55/55 100% 65/65 100% 40/40 100% 9 65/65 100% 55/55 100% 55/55 100% 55/55 100% 65/65 100% 40/40 100% 65/65 100% 55/55 100% 55/55 100% 55/55 100% 65/65 100% 40/40 100% PAGE: 1 10 11 65/65 100% 55/55 100% 55/55 100% 55/55 100% 65/65 100% 40/40 100% 65/65 100% 55/55 100% 55/55 100% 55/55 100% 65/65 100% 40/40 100% 132 TABLE 4 Survival Data Covance 6329-183 3M T-6295 GROUP AND DOSE LEVEL (PPM) 1 0 2 500 3 2 4 5 5 20 6 20 WEEK: 12 65/65 100% 55/55 100% 55/55 100% 55/55 100% 65/65 100% 40/40 100% 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS 13 65/65 100% 55/55 100% 55/55 100% 55/55 100% 65/65 100% 40/40 100% 14 60/60 100% 50/50 100% 50/50 100% 50/50 100% 60/60 100% 40/40 100% 15 60/60 100% 50/50 100% 50/50 100% 50/50 100% 60/60 100% 40/40 100% 16 17 MALE 60/60 100% 50/50 100% 50/50 100% 50/50 100% 60/60 100% 40/40 100% 60/60 100% 50/50 100% 50/50 100% 50/50 100% 60/60 100% 40/40 100% 18 60/60 100% 50/50 100% 50/50 100% 50/50 100% 60/60 100% 40/40 100% 19 60/60 100% 49/50 98% 50/50 100% 50/50 100% 60/60 100% 40/40 100% 20 60/60 100% 49/50 98% 50/50 100% 50/50 100% 60/60 100% 40/40 100% 21 60/60 100% 49/50 98% 50/50 100% 50/50 100% 60/60 100% 40/40 100% PAGE: 2 22 23 60/60 100% 49/50 98% 50/50 100% 50/50 100% 60/60 100% 40/40 100% 59/60 98% 49/50 98% 50/50 100% 50/50 100% 60/60 100% 40/40 100% 133 TABLE 4 Survival Data Covance 6329-183 3M T-6295 GROUP AND DOSE LEVEL (PPM) 1 0 2 500 3 2 4 5 5 20 6 20 WEEK: 24 59/60 98% 49/50 98% 50/50 100% 50/50 100% 60/60 100% 40/40 100% 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS 25 59/60 98% 49/50 98% 50/50 100% 50/50 100% 60/60 100% 40/40 100% 26 59/60 98% 49/50 98% 50/50 100% 50/50 100% 60/60 100% 40/40 100% 27 59/60 98% 49/50 98% 50/50 100% 50/50 100% 60/60 100% 40/40 100% 28 29 MALE 59/60 98% 49/50 98% 50/50 100% 50/50 100% 60/60 100% 40/40 100% 59/60 98% 49/50 98% 50/50 100% 50/50 100% 60/60 100% 40/40 100% 30 59/60 98% 49/50 98% 50/50 100% 50/50 100% 60/60 100% 40/40 100% 31 59/60 98% 49/50 98% 50/50 100% 50/50 100% 60/60 100% 40/40 100% 32 59/60 98% 49/50 98% 50/50 100% 50/50 100% 60/60 100% 40/40 100% 33 59/60 98% 49/50 98% 50/50 100% 50/50 100% 60/60 100% 40/40 100% PAGE: 3 34 35 59/60 98% 49/50 98% 50/50 100% 50/50 100% 60/60 100% 40/40 100% 59/60 98% 49/50 98% 50/50 100% 50/50 100% 60/60 100% 40/40 100% 134 TABLE 4 Survival Data Covance 6329-183 3M T-6295 GROUP AND DOSE LEVEL (PPM) 1 0 2 500 3 2 4 5 5 20 6 20 WEEK: 36 59/60 98% 49/50 98% 50/50 100% 50/50 100% 59/60 98% 40/40 100% 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS 37 59/60 98% 49/50 98% 50/50 100% 50/50 100% 59/60 98% 40/40 100% 38 59/60 98% 49/50 98% 50/50 100% 50/50 100% 59/60 98% 40/40 100% 39 59/60 98% 48/50 96% 50/50 100% 50/50 100% 59/60 98% 40/40 100% 40 41 MALE 59/60 98% 48/50 96% 50/50 100% 50/50 100% 59/60 98% 40/40 100% 59/60 98% 48/50 96% 50/50 100% 50/50 100% 59/60 98% 40/40 100% 42 59/60 98% 48/50 96% 50/50 100% 50/50 100% 59/60 98% 40/40 100% 43 59/60 98% 48/50 96% 50/50 100% 50/50 100% 59/60 98% 40/40 100% 44 59/60 98% 48/50 96% 50/50 100% 50/50 100% 59/60 98% 40/40 100% 45 59/60 98% 48/50 96% 50/50 100% 50/50 100% 59/60 98% 40/40 100% PAGE: 4 46 47 59/60 98% 48/50 96% 50/50 100% 50/50 100% 59/60 98% 40/40 100% 58/60 97% 48/50 96% 50/50 100% 50/50 100% 58/60 97% 40/40 100% 135 TABLE 4 Survival Data Covance 6329-183 3M T-6295 GROUP AND DOSE LEVEL (PPM) 1 0 2 .500 3 2 4 5 5 20 6 20 WEEK: 48 58/60 97% 48/50 96% 50/50 100% 50/50 100% 58/60 97% 40/40 100% 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS 49 58/60 97% 48/50 96% 50/50 100% 50/50 100% 58/60 97% 40/40 100% 50 58/60 97% 48/50 96% 50/50 100% 50/50 100% 58/60 97% 40/40 100% 51 58/60 97% 48/50 96% 50/50 100% 50/50 100% 58/60 97% 40/40 100% 52 53 MALE 58/60 97% 48/50 96% 50/50 100% 49/50 98% 57/60 95% 40/40 100% 48/50 96% 48/50 96% 50/50 100% 47/50 94% 48/51 94% 40/40 100% 54 48/50 96% 48/50 96% 50/50 100% 47/50 94% 48/51 94% 40/40 100% 55 48/50 96% 47/50 94% 49/50 98% 47/50 94% 48/51 94% 39/40 98% 56 48/50 96% 47/50 94% 48/50 96% 47/50 94% 48/51 94% 39/40 98% 57 48/50 96% 47/50 94% 48/50 96% 47/50 94% 47/51 92% 39/40 98% PAGE: 5 58 59 48/50 96% 47/50 94% 48/50 96% 47/50 94% 46/51 90% 39/40 98% 48/50 96% 47/50 94% 48/50 96% 47/50 94% 46/51 90% 39/40 98% 136 TABLE 4 Survival Data Covance 6329-183 3M T-6295 GROUP AND DOSE LEVEL (PPM) 1 0 2 .500 3 2 4 5 5 20 6 20 WEEK: 60 47/50 94% 46/50 92% 48/50 96% 47/50 94% 46/51 90% 39/40 98% 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS 61 47/50 94% 45/50 90% 48/50 96% 46/50 92% 46/51 90% 39/40 98% 62 47/50 94% 45/50 90% 48/50 96% 46/50 92% 45/51 88% 39/40 98% 63 44/50 88% 45/50 90% 48/50 96% 46/50 92% 45/51 88% 39/40 98% 64 65 MALE 44/50 88% 45/50 90% 48/50 96% 46/50 92% 44/51 86% 39/40 98% 44/50 88% 45/50 90% 48/50 96% 46/50 92% 44/51 86% 37/40 93% 66 44/50 88% 45/50 90% 48/50 96% 46/50 92% 44/51 86% 37/40 93% 67 44/50 88% 45/50 90% 48/50 96% 45/50 90% 44/51 86% 37/40 93% 68 43/50 86% 45/50 90% 48/50 96% 45/50 90% 44/51 86% 35/40 88% 69 43/50 86% 45/50 90% 48/50 96% 45/50 90% 44/51 86% 34/40 85% PAGE: 6 70 71 42/50 84% 45/50 90% 48/50 96% 44/50 88% 44/51 86% 32/40 80% 42/50 84% 44/50 88% 48/50 96% 44/50 88% 44/51 86% 32/40 80% 137 TABLE 4 Survival Data Covance 6329-183 3M T-6295 GROUP AND DOSE LEVEL (PPM) 1 0 2 .500 3 2 4 5 5 20 6 20 WEEK: 72 41/50 82% 44/50 88% 47/50 94% 44/50 88% 43/51 84% 31/40 78% 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS 73 41/50 82% 42/50 84% 47/50 94% 44/50 88% 43/51 84% 31/40 78% 74 40/50 80% 42/50 84% 46/50 92% 44/50 88% 42/51 82% 31/40 78% 75 40/50 80% 42/50 84% 46/50 92% 44/50 88% 42/51 82% 30/40 75% 76 77 MALE 40/50 80% 42/50 84% 45/50 90% 44/50 88% 41/51 80% 30/40 75% 38/50 76% 42/50 84% 45/50 90% 44/50 88% 40/51 78% 29/40 73% 78 37/50 74% 40/50 80% 44/50 88% 44/50 88% 40/51 78% 29/40 73% 79 35/50 70% 37/50 74% 41/50 82% 41/50 82% 39/51 76% 29/40 73% 80 31/50 62% 37/50 74% 39/50 78% 40/50 80% 38/51 75% 29/40 73% 81 31/50 62% 36/50 72% 38/50 76% 40/50 80% 37/51 73% 28/40 70% PAGE: 7 82 83 31/50 62% 36/50 72% 38/50 76% 40/50 80% 37/51 73% 28/40 70% 30/50 60% 35/50 70% 38/50 76% 38/50 76% 37/51 73% 25/40 63% 138 TABLE 4 Survival Data Covance 6329-183 3M T-6295 GROUP AND DOSE LEVEL (PPM) 1 0 2 .500 3 2 4 5 5 20 6 20 WEEK: 84 27/50 54% 32/50 64% 36/50 72% 37/50 74% 37/51 73% 25/40 63% 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS 85 26/50 52% 32/50 64% 35/50 70% 37/50 74% 37/51 73% 24/40 60% 86 26/50 52% 30/50 60% 35/50 70% 37/50 74% 36/51 71% 24/40 60% 87 26/50 52% 30/50 60% 32/50 64% 36/50 72% 36/51 71% 24/40 60% 88 89 MALE 25/50 50% 26/50 52% 31/50 62% 36/50 72% 35/51 69% 24/40 60% 24/50 48% 26/50 52% 31/50 62% 35/50 70% 35/51 69% 23/40 58% 90 24/50 48% 25/50 50% 30/50 60% 32/50 64% 35/51 69% 23/40 58% 91 22/50 44% 23/50 46% 29/50 58% 29/50 58% 35/51 69% 21/40 53% 92 22/50 44% 22/50 44% 29/50 58% 27/50 54% 35/51 69% 21/40 53% 93 22/50 44% 22/50 44% 28/50 56% 27/50 54% 35/51 69% 21/40 53% PAGE: 8 94 95 21/50 42% 22/50 44% 26/50 52% 27/50 54% 33/51 65% 20/40 50% 21/50 42% 22/50 44% 23/50 46% 27/50 54% 32/51 63% 18/40 45% 139 TABLE 4 Survival Data GROUP AND DOSE LEVEL (PPM) 1 0 2 .500 3 2 4 5 5 20 6 20 WEEK: 96 19/50 38% 19/50 38% 23/50 46% 27/50 54% 32/51 63% 18/40 45% 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS 97 19/50 38% 18/50 36% 21/50 42% 27/50 54% 31/51 61% 16/40 40% 98 17/50 34% 18/50 36% 20/50 40% 26/50 52% 30/51 59% 15/40 38% 99 16/50 32% 18/50 36% 19/50 38% 26/50 52% 28/51 55% 14/40 35% 100 101 MALE 15/50 30% 17/50 34% 19/50 38% 26/50 52% 27/51 53% 14/40 35% 14/50 28% 14/50 28% 19/50 38% 26/50 52% 25/51 49% 13/40 33% 102 14/50 28% 14/50 28% 18/50 36% 26/50 52% 25/51 49% 13/40 33% 103 13/50 26% 13/50 26% 17/50 34% 25/50 50% 24/51 47% 11/40 27% 104 11/50 22% 11/50 22% 17/50 34% 25/50 50% 23/51 45% 11/40 27% Covance 6329-183 3M T-6295 PAGE: 9 140 TABLE 4 Survival Data Covance 6329-183 3M T-6295 GROUP AND DOSE LEVEL (PPM) 1 0 2 .500 3 2 4 5 5 20 6 20 WEEK: START 70/70 100% 60/60 100% 60/60 100% 60/60 100% 70/70 100% 40/40 100% 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS 1 70/70 100% 60/60 100% 60/60 100% 60/60 100% 70/70 100% 40/40 100% 2 70/70 100% 60/60 100% 60/60 100% 60/60 100% 70/70 100% 40/40 100% 3 70/70 100% 60/60 100% 60/60 100% 60/60 100% 70/70 100% 40/40 100% 45 FEMALE 65/65 100% 55/55 100% 55/55 100% 55/55 100% 65/65 100% 40/40 100% 65/65 100% 55/55 100% 55/55 100% 55/55 100% 65/65 100% 40/40 100% 6 65/65 100% 55/55 100% 55/55 100% 55/55 100% 65/65 100% 40/40 100% 7 65/65 100% 55/55 100% 55/55 100% 55/55 100% 65/65 100% 40/40 100% 8 65/65 100% 55/55 100% 55/55 100% 55/55 100% 65/65 100% 40/40 100% 9 65/65 100% 55/55 100% 54/55 98% 55/55 100% 65/65 100% 40/40 100% PAGE: 10 10 11 65/65 100% 55/55 100% 54/55 98% 55/55 100% 65/65 100% 40/40 100% 65/65 100% 55/55 100% 54/55 98% 55/55 100% 65/65 100% 40/40 100% 141 TABLE 4 Survival Data Covance 6329-183 3M T-6295 GROUP AND DOSE LEVEL (PPM) 1 0 2 .500 3 2 4 5 5 20 6 20 WEEK: 12 65/65 100% 55/55 100% 54/55 98% 55/55 100% 65/65 100% 40/40 100% 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS 13 65/65 100% 55/55 100% 54/55 98% 55/55 100% 65/65 100% 40/40 100% 14 60/60 100% 50/50 100% 49/50 98% 50/50 100% 60/60 100% 40/40 100% 15 60/60 100% 50/50 100% 49/50 98% 50/50 100% 60/60 100% 40/40 100% 16 17 FEMALE 60/60 100% 50/50 100% 49/50 98% 50/50 100% 60/60 100% 40/40 100% 60/60 100% 50/50 100% 49/50 98% 50/50 100% 60/60 100% 40/40 100% 18 60/60 100% 50/50 100% 49/50 98% 50/50 100% 60/60 100% 40/40 100% 19 60/60 100% 50/50 100% 49/50 98% 50/50 100% 60/60 100% 40/40 100% 20 60/60 100% 50/50 100% 49/50 98% 50/50 100% 60/60 100% 40/40 100% 21 60/60 100% 50/50 100% 49/50 98% 50/50 100% 60/60 100% 40/40 100% PAGE: 11 22 23 60/60 100% 50/50 100% 49/50 98% 50/50 100% 60/60 100% 40/40 100% 60/60 100% 50/50 100% 49/50 98% 50/50 100% 60/60 100% 40/40 100% 142 TABLE 4 Survival Data Covance 6329-183 3M T-6295 GROUP AND DOSE LEVEL (PPM) 10 2 .500 23 4 5 205 206 WEEK: 24 6100/06%0 5100/05%0 49/50 98% 50/50 100% 6100/06%0 40/40 100% 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS 25 6100/06%0 5100/05%0 49/50 98% 50/50 100% 6100/06%0 40/40 100% 26 6100/06%0 5100/05%0 49/50 98% 50/50 100% 6100/06%0 40/40 100% 27 6100/06%0 5100/05%0 49/50 98% 50/50 100% 6100/06%0 40/40 100% 28 29 FEMALE 6100/06%0 5100/05%0 49/50 98% 50/50 100% 6100/06%0 40/40 100% 6100/06%0 5100/05%0 49/50 98% 50/50 100% 6100/06%0 40/40 100% 30 6100/06%0 5100/05%0 49/50 98% 50/50 100% 6100/06%0 40/40 100% 31 6100/06%0 5100/05%0 49/50 98% 49/50 98% 6100/06%0 40/40 100% 32 6100/06%0 5100/05%0 49/50 98% 49/50 98% 6100/06%0 40/40 100% 33 6100/06%0 49/50 98% 49/50 98% 49/50 98% 6100/06%0 40/40 100% PAGE: 12 34 35 6100/06%0 49/50 98% 49/50 98% 49/50 98% 6100/06%0 40/40 100% 6100/06%0 49/50 98% 49/50 98% 49/50 98% 6100/06%0 40/40 100% 143 TABLE 4 Survival Data Covance 6329-183 3M T-6295 GROUP AND DOSE LEVEL (PPM) 1 0 2 .500 3 2 4 5 5 20 6 20 WEEK: 36 60/60 100% 49/50 98% 49/50 98% 49/50 98% 60/60 100% 40/40 100% 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS 37 60/60 100% 49/50 98% 49/50 98% 49/50 98% 60/60 100% 40/40 100% 38 60/60 100% 49/50 98% 49/50 98% 49/50 98% 60/60 100% 40/40 100% 39 60/60 100% 49/50 98% 49/50 98% 49/50 98% 59/60 98% 40/40 100% 40 41 FEMALE 60/60 100% 49/50 98% 49/50 98% 49/50 98% 59/60 98% 40/40 100% 60/60 100% 48/50 96% 49/50 98% 49/50 98% 59/60 98% 39/40 98% 42 60/60 100% 48/50 96% 49/50 98% 49/50 98% 59/60 98% 39/40 98% 43 60/60 100% 48/50 96% 49/50 98% 49/50 98% 59/60 98% 39/40 98% 44 60/60 100% 48/50 96% 49/50 98% 49/50 98% 59/60 98% 39/40 98% 45 60/60 100% 48/50 96% 48/50 96% 49/50 98% 59/60 98% 39/40 98% PAGE: 13 46 47 60/60 100% 48/50 96% 47/50 94% 49/50 98% 59/60 98% 39/40 98% 60/6 100% 48/5 96% 47/5 94% 49/5 98% 59/6 98% 39/4 98% 144 TABLE 4 Survival Data Covance 6329-183 3M T-6295 GROUP AND DOSE LEVEL (PPM) 1 0 2 .500 3 2 4 5 5 20 6 20 WEEK: 48 60/60 100% 48/50 96% 47/50 94% 49/50 98% 59/60 98% 39/40 98% 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS 49 60/60 100% 48/50 96% 47/50 94% 49/50 98% 59/60 98% 39/40 98% 50 60/60 100% 47/50 94% 47/50 94% 49/50 98% 59/60 98% 39/40 98% 51 60/60 100% 47/50 94% 47/50 94% 49/50 98% 59/60 98% 39/40 98% 52 53 FEMALE 60/60 100% 47/50 94% 47/50 94% 48/50 96% 59/60 98% 39/40 98% 50/50 100% 47/50 94% 47/50 94% 48/50 96% 49/50 98% 39/40 98% 54 50/50 100% 47/50 94% 47/50 94% 48/50 96% 49/50 98% 39/40 98% 55 50/50 100% 47/50 94% 47/50 94% 48/50 96% 48/50 96% 39/40 98% 56 50/50 100% 47/50 94% 47/50 94% 48/50 96% 48/50 96% 39/40 98% 57 49/50 98% 47/50 94% 47/50 94% 48/50 96% 48/50 96% 39/40 98% PAGE: 14 58 59 49/50 98% 47/50 94% 47/50 94% 48/50 96% 47/50 94% 39/40 98% 49/5 98% 47/5 94% 47/5 94% 48/5 96% 47/5 94% 39/4 98% 145 TABLE 4 Survival Data Covance 6329-183 3M T-6295 GROUP AND DOSE LEVEL (PPM) 1 0 2 .500 3 2 4 5 5 20 6 20 WEEK: 60 48/50 96% 47/50 94% 47/50 94% 48/50 96% 47/50 94% 39/40 98% 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS 61 48/50 96% 47/50 94% 47/50 94% 48/50 96% 47/50 94% 39/40 98% 62 48/50 96% 47/50 94% 47/50 94% 48/50 96% 47/50 94% 39/40 98% 63 48/50 96% 46/50 92% 46/50 92% 48/50 96% 46/50 92% 39/40 98% 64 65 FEMALE 48/50 96% 46/50 92% 46/50 92% 48/50 96% 46/50 92% 39/40 98% 47/50 94% 45/50 90% 46/50 92% 48/50 96% 46/50 92% 38/40 95% 66 47/50 94% 44/50 88% 42/50 84% 47/50 94% 45/50 90% 38/40 95% 67 47/50 94% 44/50 88% 42/50 84% 47/50 94% 45/50 90% 38/40 95% 68 47/50 94% 44/50 88% 42/50 84% 47/50 94% 44/50 88% 38/40 95% 69 47/50 94% 43/50 86% 42/50 84% 47/50 94% 44/50 88% 38/40 95% PAGE: 15 70 71 47/50 94% 42/50 84% 41/50 82% 47/50 94% 44/50 88% 38/40 95% 47/5 94% 41/5 82% 40/5 80% 47/5 94% 44/5 88% 37/4 93% 146 TABLE 4 Survival Data Covance 6329-183 3M T-6295 GROUP AND DOSE LEVEL (PPM) 1 0 2 .500 3 2 4 5 5 20 6 20 WEEK: 72 45/50 90% 39/50 78% 39/50 78% 45/50 90% 44/50 88% 37/40 93% 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS 73 44/50 88% 39/50 78% 39/50 78% 45/50 90% 44/50 88% 37/40 93% 74 44/50 88% 39/50 78% 39/50 78% 45/50 90% 44/50 88% 37/40 93% 75 44/50 88% 39/50 78% 38/50 76% 44/50 88% 43/50 86% 36/40 90% 76 77 FEMALE 44/50 88% 39/50 78% 37/50 74% 44/50 88% 42/50 84% 35/40 88% 44/50 88% 39/50 78% 37/50 74% 41/50 82% 42/50 84% 35/40 88% 78 44/50 88% 39/50 78% 37/50 74% 40/50 80% 41/50 82% 35/40 88% 79 41/50 82% 39/50 78% 37/50 74% 40/50 80% 40/50 80% 35/40 88% 80 41/50 82% 39/50 78% 35/50 70% 39/50 78% 40/50 80% 35/40 88% 81 39/50 78% 39/50 78% 33/50 66% 39/50 78% 40/50 80% 35/40 88% PAGE: 16 82 83 39/50 78% 38/50 76% 31/50 62% 39/50 78% 40/50 80% 35/40 88% 37/5 74% 38/5 76% 28/5 56% 38/5 76% 40/5 80% 34/4 85% 147 TABLE 4 Survival Data Covance 6329-183 3M T-6295 GROUP AND DOSE LEVEL (PPM) 1 0 2 .500 3 2 4 5 5 20 6 20 WEEK: 84 37/50 74% 37/50 74% 26/50 52% 37/50 74% 40/50 80% 32/40 80% 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS 85 35/50 70% 37/50 74% 25/50 50% 37/50 74% 40/50 80% 32/40 80% 86 35/50 70% 37/50 74% 23/50 46% 37/50 74% 39/50 78% 31/40 78% 87 34/50 68% 35/50 70% 23/50 46% 37/50 74% 39/50 78% 30/40 75% 88 89 FEMALE 33/50 66% 34/50 68% 23/50 46% 37/50 74% 38/50 76% 29/40 73% 31/50 62% 32/50 64% 23/50 46% 35/50 70% 38/50 76% 29/40 73% 90 31/50 62% 31/50 62% 22/50 44% 33/50 66% 38/50 76% 27/40 68% 91 31/50 62% 31/50 62% 19/50 38% 33/50 66% 37/50 74% 25/40 63% 92 31/50 62% 29/50 58% 16/50 32% 33/50 66% 33/50 66% 23/40 58% 93 28/50 56% 29/50 58% 16/50 32% 31/50 62% 32/50 64% 23/40 58% PAGE: 17 94 95 28/50 56% 26/50 52% 16/50 32% 29/50 58% 31/50 62% 23/40 58% 28/5 56% 26/5 52% 15/5 30% 28/5 56% 31/5 62% 23/4 58% 148 TABLE 4 Survival Data GROUP AND DOSE LEVEL (PPM) 1 0 2 .500 3 2 4 5 5 20 6 20 WEEK: 96 26/50 52% 25/50 50% 14/50 28% 25/50 50% 30/50 60% 22/40 55% 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS 97 26/50 52% 24/50 48% 13/50 26% 24/50 48% 30/50 60% 22/40 55% 98 26/50 52% 21/50 42% 12/50 24% 23/50 46% 30/50 60% 21/40 53% 99 26/50 52% 19/50 38% 12/50 24% 20/50 40% 30/50 60% 21/40 53% 100 101 FEMALE 26/50 52% 19/50 38% 12/50 24% 19/50 38% 27/50 54% 21/40 53% 25/50 50% 18/50 36% 11/50 22% 18/50 36% 27/50 54% 21/40 53% 102 25/50 50% 16/50 32% 10/50 20% 18/50 36% 26/50 52% 21/40 53% 103 25/50 50% 15/50 30% 17/50 34% 26/50 52% 21/40 53% 104 25/5 50% 15/5 30% 17/5 34% 26/5 52% 19/4 48% Covance 6329-183 3M T-6295 PAGE: 18 149 DAYS 1-736 CATEGORY KEYWORD QUALIFIER *** TOP OF LIST *** APPEARANCE BENT TAIL CONVULSIONS HEAD TILT LEFT RIGHT HUNCHED LIMITED USE LIMB-FRONT-LEFT LIMB-HIND-LEFT LIMB-HIND-RIGHT LIMBS-ALL LIMBS-HIND PAW-HIND-LEFT PAW-HIND-RIGHT PAWS-ALL PAWS-FRONT PAWS-HIND MALOCCLUSION MISSING TAIL-DISTAL TEETH OBES PARALYSIS LIMBS-HIND Table 5 Summary of Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS SEX: GROUP : DOSE: NUMBER: NUMBER OF ANIMALS AFFECTED ------------ MALE 123456 0 0.5 2 5 20 20 70 60 60 60 70 40 01 0001 0001 01 01 01 01 4 01 002 333222 01 0000 00001 0 2 0001 0 1 0001 1 04 01 2 2 00001 1 1 00000 00001 0 01 0000 1 00000 12 7 9 9 11 10 000000 251 1 52 1 1 01 32 01 0000 150 Covance 6329-183 3M T-6295 PAGE: 1 DAYS 1-736 CATEGORY KEYWORD QUALIFIER APPEARANCE PROLAPSED VAGINA SWOLLEN DORSAL DIGIT(S)-HIND-LEFT DIGIT(S)-HIND-RIGHT LIMB-HIND-LEFT LIMB-HIND-RIGHT LIMBS-HIND MOUTH NOSE PERI-ORAL PAW-FRONT-RIGHT PAW-HIND-LEFT PAW-HIND-RIGHT PAWS-HIND TAIL TAIL-DISTAL TAIL-PROXIMAL VENTRAL-ABDOMINAL VENTRAL-ABDOMINAL-LEFT VAGINA TESTES TESTIS-LEFT Table 5 Summary of Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS SEX: GROUP : DOSE: NUMBER: NUMBER OF ANIMALS AFFECTED ------------ MALE 123456 0 0.5 2 5 20 20 70 60 60 60 70 40 000000 000000 000000 1 00000 002 01 2 1 00002 00001 0 01 0000 1 00001 001 000 0001 00 1 32 01 5 4 02 2 34 1 0001 0 1 00000 000000 001 000 1 003 00 00001 0 000000 001 000 000001 151 Covance 6329-183 3M T-6295 PAGE: 2 DAYS 1-736 CATEGORY KEYWORD QUALIFIER APPEARANCE THIN TREMORS LIMBS-ALL ENTIRE BODY HEAD BEHAVIOR AGGRESSIVE <UNSPECIFIED> ATAXIC <UNSPECIFIED> MODERATE CIRCLING LEFT RIGHT HYPOACTIVE <UNSPECIFIED> MILD MODERATE SEVERE RECUMBENT DORSAL LATERAL STERNAL Table 5 Summary of Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS SEX: GROUP : DOSE: NUMBER: NUMBER OF ANIMALS AFFECTED ------------ MALE 123456 0 0.5 2 5 20 20 70 60 60 60 70 40 15 21 17 10 12 11 1 00000 00001 0 01 0000 001 000 44 01 1 2 000000 000000 000000 10 13 14 11 10 10 000000 1 00001 01 0000 000001 4 32 2 02 1 1 001 2 152 Covance 6329-183 3M T-6295 PAGE: 3 DAYS 1-736 CATEGORY KEYWORD QUALIFIER BEHAVIOR SENSITIVE TO TOUCH COLD TO TOUCH LIMBS-ALL LIMBS-HIND TAIL ENTIRE BODY DISCHARGE GENITAL RED IN COLOR CLEAR ORAL <UNSPECIFIED> MILD MODERATE NASAL RED IN COLOR UNKNOWN RED IN COLOR EXCRETION DISCOLORED FECES RED IN COLOR YELLOW IN COLOR Table 5 Summary of Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS SEX: GROUP : DOSE: NUMBER: NUMBER OF ANIMALS AFFECTED -- MALE--1 23456 0 0.5 2 5 20 20 70 60 60 60 70 40 01 02 00 00001 1 01 0000 00001 0 241 324 61 2241 1 00001 0001 00 000000 2 01 2 02 002 000 1 00000 000000 153 Covance 6329-183 3M T-6295 PAGE: 4 DAYS 1-736 CATEGORY KEYWORD QUALIFIER EXCRETION DISCOLORED URINE RED IN COLOR FEW FECES LIQUID FECES NO FECES NONFORMED FECES EYES CLEAR DISCHARGE EYE-LEFT EYE-RIGHT EYES DILATED PUPIL EYES DISCOLORED/LIGHT RED EYE-LEFT EYE-RIGHT EYES OPAQUE EYE-LEFT PALE EYE-RIGHT EYES PROTRUDING EYE-LEFT Table 5 Summary of Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS SEX: GROUP : DOSE: NUMBER: NUMBER OF ANIMALS AFFECTED -- MALE--1 23456 0 0.5 2 5 20 20 70 60 60 60 70 40 001 000 16 23 16 16 18 16 0001 01 623534 232324 1 21 000 01 01 00 02 0000 001 000 000000 0002 00 0001 00 001 000 000000 01 02 00 001 000 154 Covance 6329-183 3M T-6295 PAGE: 5 DAYS 1-736 CATEGORY KEYWORD QUALIFIER EYES PROTRUDING EYE-RIGHT EYES RED DISCHARGE EYE-LEFT EYE-RIGHT EYES SCAB EYE-LEFT EYE-RIGHT SQUINTED PERI-ORBITAL PERI-ORBITAL-RIGHT RESPIRATION AUDIBLE IRREGULAR LABORED SKIN & PELAGE ALOPECIA BROWN HAIR COAT PERINEAL AREA SCROTUM Table 5 Summary of Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS SEX: GROUP : DOSE: NUMBER: NUMBER OF ANIMALS AFFECTED ------------ MALE 123456 0 0.5 2 5 20 20 70 60 60 60 70 40 000000 00001 0 2251 1 2 1 22633 553523 001 000 02 0000 7 03222 001 2 00 531 1 1 3 621 1 1 3 2421 22 000000 1 002 1 0 1 1 0001 155 Covance 6329-183 3M T-6295 PAGE: 6 DAYS 1-736 CATEGORY KEYWORD QUALIFIER SKIN & PELAGE BROWN HAIR COAT VENTRAL-ABDOMINAL BLUE SKIN PAWS-ALL TAIL VENTRAL-ABDOMINAL VENTRAL-ABDOMINAL-LEFT VENTRAL-THORACIC-LEFT BROWN SKIN VENTRAL-ABDOMINAL BLACK SKIN DORSAL TAIL VENTRAL-ABDOMINAL VENTRAL-ABDOMINAL-LEFT VENTRAL-ABDOMINAL-RIGHT VENTRAL-CERVICAL-RIGHT VENTRAL-THORACIC VENTRAL-THORACIC-LEFT PALE ENTIRE BODY RED HAIR COAT DORSAL-CERVICAL HEAD-ENTIRE HEAD-CRANIAL Table 5 Summary of Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS SEX: GROUP : DOSE: NUMBER: NUMBER OF ANIMALS AFFECTED ------------ MALE 1 23 4 5 6 0 0.5 2 5 20 20 70 60 60 60 70 40 010011 000000 000000 000000 000000 000000 000000 00 00 00 10 10 00 000010 010000 000000 00 00 00 00 00 00 000000 05 5 3 12 000000 543333 000000 156 Covance 6329-183 3M T-6295 PAGE: 7 DAYS 1-736 CATEGORY KEYWORD QUALIFIER SKIN & PELAGE RED HAIR COAT HEAD-MAXILLARY-LEFT HEAD-MAXILLARY-RIGHT LATERAL-ABDOMINAL-RIGHT LATERAL-CERVICAL-RIGHT LATERA-RIGHT LATERAL-THORACIC-LEFT PERINEAL AREA SHOULDER-RIGHT VENTRAL-ABDOMINAL ROUGH HAIR COAT RED SKIN EARS HEAD-MAXILLARY-RIGHT PAW-HIND-RIGHT SORE/SCAB AXILLARY REGION-LEFT AXILLARY REGION-RIGHT DORSAL DORSAL-CERVICAL DORSAL-CERVICAL-LEFT DORSAL-CERVICAL-RIGHT DORSAL-THORACIC DORSAL-THORACIC-LEFT DORSAL-THORACIC-RIGHT Table 5 Summary of Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS SEX: GROUP : DOSE: NUMBER: NUMBER OF ANIMALS AFFECTED ------------ MALE 123456 0 0.5 2 5 20 20 70 60 60 60 70 40 0001 00 0001 00 0001 00 000000 000000 000000 000000 00001 0 2 01 1 1 0 766434 000000 1 00000 000001 0001 2 0 000001 3 7 04 4 2 01 0000 001 01 0 000000 01 1 1 00 00002 0 01 0001 157 Covance 6329-183 3M T-6295 PAGE: 8 DAYS 1-736 CATEGORY KEYWORD QUALIFIER SKIN & PELAGE SORE/SCAB EAR-LEFT EAR-RIGHT HEAD-ENTIRE HEAD-CRANIAL HEAD-MAXILLARY-LEFT HEAD-MAXILLARY-RIGHT LATERAL-ABDOMINAL-LEFT LATERAL-ABDOMINAL-RIGHT LATERAL-CERVICAL-RIGHT LIMB-FRONT-RIGHT LIMB-HIND-RIGHT LIMBS-HIND LATERAL-LEFT LATERAL-THORACIC-LEFT MOUTH PAW-HIND-LEFT PAW-HIND-RIGHT PAWS-HIND SHOULDER-LEFT SHOULDER-RIGHT TAIL TAIL-DISTAL TAIL-MID TAIL-PROXIMAL Table 5 Summary of Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS SEX: GROUP : DOSE: NUMBER: NUMBER OF ANIMALS AFFECTED ------------ MALE 123456 0 0.5 2 5 20 20 70 60 60 60 70 40 001 000 000001 01 001 0 001 000 00001 0 1 001 00 001 01 0 001 000 001 000 0002 1 0 000000 000000 0001 00 000000 0001 01 221 322 1 02 3 1 2 1 002 01 01 1 000 2 1 1 001 000000 000000 001 000 2 00000 158 Covance 6329-183 3M T-6295 PAGE: 9 Table 5 Summary of Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS DAYS 1-736 CATEGORY KEYWORD QUALIFIER SEX: GROUP : DOSE: NUMBER: NUMBER OF ANIMALS AFFECTED ------------ MALE 123456 0 0.5 2 5 20 20 70 60 60 60 70 40 SKIN & PELAGE SORE/SCAB VENTRAL-ABDOMINAL VENTRAL-ABDOMINAL-LEFT VENTRAL-ABDOMINAL-RIGHT VENTRAL-CERVICAL VENTRAL-CERVICAL-LEFT VENTRAL-CERVICAL-RIGHT VENTRAL-THORACIC VENTRAL-THORACIC-LEFT VENTRAL-THORACIC-RIGHT WARM TO TOUCH LIMBS-HIND YELLOW HAIR COAT YELLOW SKIN PAWS-ALL TAIL BLACK HAIR COAT - PERINEAL COLD TO TOUCH 21 4265 0322 32 04 34 81 02 0000 000000 000000 000000 000000 000000 000000 42 01 2 0 000000 000000 0001 00 000001 Covance 6329-183 3M T-6295 PAGE: 10 159 DAYS 1-736 CATEGORY KEYWORD QUALIFIER *** TOP OF LIST *** APPEARANCE BENT TAIL CONVULSIONS HEAD TILT LEFT RIGHT HUNCHED LIMITED USE LIMB-FRONT-LEFT LIMB-HIND-LEFT LIMB-HIND-RIGHT LIMBS-ALL LIMBS-HIND PAW-HIND-LEFT PAW-HIND-RIGHT PAWS-ALL PAWS-FRONT PAWS-HIND MALOCCLUSION MISSING TAIL-DISTAL TEETH OBES PARALYSIS LIMBS-HIND Table 5 Summary of Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS SEX: GROUP : DOSE: NUMBER: NUMBER OF ANIMALS AFFECTED --FEMALE-1 23456 0 0.5 2 5 20 20 70 60 60 60 70 40 00000 1 01 01 54762 23552 34565 00000 1 1 000 00000 00000 00000 00000 00000 00000 00000 00000 16 13 11 12 10 1 01 1 0 25235 2 02 00 00000 0 2 3 1 3 0 0 0 0 0 0 0 0 0 0 8 0 1 0 0 160 Covance 6329-183 3M T-6295 PAGE: 11 DAYS 1-736 CATEGORY KEYWORD QUALIFIER APPEARANCE PROLAPSED VAGINA SWOLLEN DORSAL DIGIT(S)-HIND-LEFT DIGIT(S)-HIND-RIGHT LIMB-HIND-LEFT LIMB-HIND-RIGHT LIMBS-HIND MOUTH NOSE PERI-ORAL PAW-FRONT-RIGHT PAW-HIND-LEFT PAW-HIND-RIGHT PAWS-HIND TAIL TAIL-DISTAL TAIL-PROXIMAL VENTRAL-ABDOMINAL VENTRAL-ABDOMINAL-LEFT VAGINA TESTES TESTIS-LEFT Table 5 Summary of Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS SEX: GROUP : DOSE: NUMBER: NUMBER OF ANIMALS AFFECTED ----------- FEMALE 123456 0 0.5 2 5 20 20 70 60 60 60 70 40 01 1 000 00001 0 1 00000 000000 1 0001 0 0001 00 000000 01 01 00 0002 00 000000 000000 1 01 02 0 1 1 1 000 000000 000000 001 000 0001 00 1 0002 0 000000 001 000 000000 000000 161 Covance 6329-183 3M T-6295 PAGE: 12 DAYS 1-736 CATEGORY KEYWORD QUALIFIER APPEARANCE THIN TREMORS LIMBS-ALL ENTIRE BODY HEAD BEHAVIOR AGGRESSIVE <UNSPECIFIED> ATAXIC <UNSPECIFIED> MODERATE CIRCLING LEFT RIGHT HYPOACTIVE <UNSPECIFIED> MILD MODERATE SEVERE RECUMBENT DORSAL LATERAL STERNAL Table 5 Summary of Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS SEX: GROUP : DOSE: NUMBER: NUMBER OF ANIMALS AFFECTED --- --FEMALE-- 12345 0 0.5 2 5 20 6 20 70 60 60 60 70 40 12 14 21 17 17 00000 001 02 00000 9 0 3 0 000000 64 9633 001 000 001 000 1 00000 8 7 16 15 7 10 001 000 01 2 01 0 001 000 001 000 31 2 031 000001 162 Covance 6329-183 3M T-6295 PAGE: 13 Table 5 Summary of Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS DAYS 1-736 CATEGORY KEYWORD QUALIFIER SEX: GROUP : DOSE: NUMBER: NUMBER OF ANIMALS AFFECTED --FEMALE-1 23456 0 0.5 2 5 20 20 70 60 60 60 70 40 BEHAVIOR SENSITIVE TO TOUCH COLD TO TOUCH LIMBS-ALL LIMBS-HIND TAIL ENTIRE BODY DISCHARGE GENITAL RED IN COLOR CLEAR ORAL <UNSPECIFIED> MILD MODERATE NASAL RED IN COLOR UNKNOWN RED IN COLOR AXILLARY LEFT MASS BLEEDING EXCRETION DISCOLORED FECES RED IN COLOR YELLOW IN COLOR 1 00000 0001 00 000000 000000 02 1 2 3 1 032 1 1 1 001 01 0 000000 001 000 4231 42 000000 0001 00 000000 00001 0 163 Covance 6329-183 3M T-6295 PAGE: 14 DAYS 1-736 CATEGORY KEYWORD QUALIFIER EXCRETION DISCOLORED URINE RED IN COLOR FEW FECES LIQUID FECES NO FECES NONFORMED FECES EYES CLEAR DISCHARGE EYE-LEFT EYE-RIGHT EYES DILATED PUPIL EYES DISCOLORED/LIGHT RED EYE-LEFT EYE-RIGHT EYES OPAQUE EYE-LEFT PALE EYE-RIGHT EYES PROTRUDING EYE-LEFT Table 5 Summary of Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS SEX: GROUP : DOSE: NUMBER: NUMBER OF ANIMALS AFFECTED --FEMALE-1 23456 0 0.5 2 5 20 20 70 60 60 60 70 40 000000 17 20 20 16 13 10 000000 677232 2 1 1 1 01 2 01 21 1 001 11 02 01 0 0 0 01 01 1 0 1 02 000 1 0001 0 1 0001 0 000001 00001 0 01 1 2 00 000000 164 Covance 6329-183 3M T-6295 PAGE: 15 DAYS 1-736 CATEGORY KEYWORD QUALIFIER EYES PROTRUDING EYE-RIGHT EYES RED DISCHARGE EYE-LEFT EYE-RIGHT EYES SCAB EYE-LEFT EYE-RIGHT SQUINTED PERI-ORBITAL PERI-ORBITAL-RIGHT SWOLLEN RIGHT EYELID RESPIRATION AUDIBLE IRREGULAR LABORED SKIN & PELAGE ALOPECIA BROWN HAIR COAT PERINEAL AREA SCROTUM Table 5 Summary of Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS SEX: GROUP : DOSE: NUMBER: NUMBER OF ANIMALS AFFECTED --- --FEMALE-- 12345 0 0.5 2 5 20 6 20 70 60 60 60 70 40 00001 0 34 1 06 0 1 1 5332 561 542 7 5 14 7 7 6 001 000 00002 0 1 01 000 003 03 0 001 000 1 052 1 2 1 1 3 01 1 121 1 1 1 2 002 3 1 2 01 01 0 000000 165 Covance 6329-183 3M T-6295 PAGE: 16 DAYS 1-736 CATEGORY KEYWORD QUALIFIER SKIN & PELAGE BROWN HAIR COAT VENTRAL-ABDOMINAL BLUE SKIN PAWS-ALL TAIL VENTRAL-ABDOMINAL VENTRAL-ABDOMINAL-LEFT VENTRAL-THORACIC-LEFT BROWN SKIN VENTRAL-ABDOMINAL BLACK SKIN DORSAL TAIL VENTRAL-ABDOMINAL VENTRAL-ABDOMINAL-LEFT VENTRAL-ABDOMINAL-RIGHT VENTRAL-CERVICAL-RIGHT VENTRAL-THORACIC VENTRAL-THORACIC-LEFT PALE ENTIRE BODY RED HAIR COAT DORSAL-CERVICAL HEAD-ENTIRE HEAD-CRANIAL Table 5 Summary of Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS SEX: GROUP : DOSE: NUMBER: NUMBER OF ANIMALS AFFECTED --- --FEMALE-- 12345 0 0.5 2 5 20 6 20 70 60 60 60 70 40 02 01 00 01 0000 01 0000 1 00000 02 002 0 001 000 001 000 01 0000 1 00000 1 00000 0001 2 1 02 0001 01 0000 1 00000 001 1 00 1 21 322 002 000 4 5 94 64 01 2 000 166 Covance 6329-183 3M T-6295 PAGE: 17 DAYS 1-736 CATEGORY KEYWORD QUALIFIER SKIN & PELAGE RED HAIR COAT HEAD-MAXILLARY-LEFT HEAD-MAXILLARY-RIGHT LATERAL-ABDOMINAL-RIGHT LATERAL-CERVICAL-RIGHT LATERA-RIGHT LATERAL-THORACIC-LEFT PERINEAL AREA SHOULDER-RIGHT VENTRAL-ABDOMINAL ROUGH HAIR COAT RED SKIN EARS HEAD-MAXILLARY-RIGHT PAW-HIND-RIGHT SORE/SCAB AXILLARY REGION-LEFT AXILLARY REGION-RIGHT DORSAL DORSAL-CERVICAL DORSAL-CERVICAL-LEFT DORSAL-CERVICAL-RIGHT DORSAL-THORACIC DORSAL-THORACIC-LEFT DORSAL-THORACIC-RIGHT Table 5 Summary of Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS SEX: GROUP : DOSE: NUMBER: NUMBER OF ANIMALS AFFECTED --- --FEMALE-- 12345 0 0.5 2 5 20 6 20 70 60 60 60 70 40 000000 000000 000000 0001 00 001 000 001 000 00001 0 000000 001 2 01 465254 001 000 000000 000000 21 31 1 2 1 1 1 1 00 1 1 001 0 01 2 01 1 00001 0 001 01 0 1 00000 000000 3 01 000 167 Covance 6329-183 3M T-6295 PAGE: 18 DAYS 1-736 CATEGORY KEYWORD QUALIFIER SKIN & PELAGE SORE/SCAB EAR-LEFT EAR-RIGHT HEAD-ENTIRE HEAD-CRANIAL HEAD-MAXILLARY-LEFT HEAD-MAXILLARY-RIGHT LATERAL-ABDOMINAL-LEFT LATERAL-ABDOMINAL-RIGHT LATERAL-CERVICAL-RIGHT LIMB-FRONT-RIGHT LIMB-HIND-RIGHT LIMBS-HIND LATERAL-LEFT LATERAL-THORACIC-LEFT MOUTH PAW-HIND-LEFT PAW-HIND-RIGHT PAWS-HIND SHOULDER-LEFT SHOULDER-RIGHT TAIL TAIL-DISTAL TAIL-MID TAIL-PROXIMAL Table 5 Summary of Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS SEX: GROUP : DOSE: NUMBER: NUMBER OF ANIMALS AFFECTED ----------- FEMALE 123456 0 0.5 2 5 20 20 70 60 60 60 70 40 000000 000000 001 01 0 001 000 0001 00 001 01 0 0001 00 0001 00 01 0000 000000 0001 00 000002 000000 1 00000 22 0031 1 1 2254 1 42221 1 1 1 141 1 00000 01 001 0 001 000 0001 00 000000 0001 00 168 Covance 6329-183 3M T-6295 PAGE: 19 DAYS 1-736 CATEGORY KEYWORD QUALIFIER SKIN & PELAGE SORE/SCAB VENTRAL-ABDOMINAL VENTRAL-ABDOMINAL-LEFT VENTRAL-ABDOMINAL-RIGHT VENTRAL-CERVICAL VENTRAL-CERVICAL-LEFT VENTRAL-CERVICAL-RIGHT VENTRAL-THORACIC VENTRAL-THORACIC-LEFT VENTRAL-THORACIC-RIGHT WARM TO TOUCH LIMBS-HIND YELLOW HAIR COAT YELLOW SKIN PAWS-ALL TAIL *** END OF LIST *** Table 5 Summary of Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS SEX: GROUP : DOSE: NUMBER: NUMBER OF ANIMALS AFFECTED --FEMALE-- 123 4 5 6 0 0.5 2 5 20 20 70 60 60 60 70 40 3 0013 0 13 2021 12 02 13 000010 111000 000110 110001 1 022 00 000001 000001 225 3 23 000100 000100 Covance 6329-183 3M T-6295 PAGE: 20 169 DAYS 1-736 CATEGORY KEYWORD QUALIFIER *** TOP OF LIST *** MASS(ES) AXILLARY REGION-LEFT AXILLARY REGION-RIGHT DORSAL DORSAL-CERVICAL-LEFT DORSAL-CERVICAL-RIGHT EAR-LEFT EAR-RIGHT HEAD-MAXILLARY-LEFT HEAD-MAXILLARY-RIGHT INGUINAL REGION-LEFT INGUINAL REGION-RIGHT LIMB-FRONT-LEFT LIMB-FRONT-RIGHT LIMB-HIND-LEFT LIMB-HIND-RIGHT MOUTH PAW-FRONT-LEFT PAW-HIND-RIGHT SHOULDER-LEFT SHOULDER-RIGHT TAIL TAIL-MID TAIL-PROXIMAL VENTRAL-ABDOMINAL Table 6 Summary of Mass Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS SEX: GROUP : DOSE: NUMBER: NUMBER OF ANIMALS AFFECTED ------------ MALE 123456 0 0.5 2 5 20 20 70 60 60 60 70 40 221 1 30 02 0002 3 10 4 8 6 3 001 01 0 000001 001 000 000001 01 1 1 01 1 02 1 00 1 1 01 33 01 02 3 0 1 00000 0002 00 000000 01 0000 0002 00 00001 0 0001 00 01 1 000 1 2 0001 001 000 001 000 6 01 000 332462 170 Covance 6329-183 3M T-6295 PAGE: 1 DAYS 1-736 CATEGORY KEYWORD QUALIFIER MASS(ES) VENTRAL-ABDOMINAL-LEFT VENTRAL-ABDOMINAL-RIGHT VENTRAL-CERVICAL VENTRAL-CERVICAL-LEFT VENTRAL-CERVICAL-RIGHT VENTRAL-THORACIC VENTRAL-THORACIC-LEFT VENTRAL-THORACIC-RIGHT RECTUM WART LIKE LESION(S) AXILLARY REGION-LEFT AXILLARY REGION-RIGHT DORSAL DORSAL-CERVICAL DORSAL-CERVICAL-LEFT DORSAL-CERVICAL-RIGHT EAR-RIGHT HEAD-MAXILLARY-LEFT HEAD-MAXILLARY-RIGHT LIMB-FRONT-RIGHT MOUTH NOSE PAW-HIND-LEFT Table 6 Summary of Mass Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS SEX: GROUP : DOSE: NUMBER: NUMBER OF ANIMALS AFFECTED ------------ MALE 123456 0 0.5 2 5 20 20 70 60 60 60 70 40 263472 0544 63 01 0000 1 01 000 000000 1 00000 0001 00 001 000 00001 0 0001 00 000001 2 2 1 2 04 000000 0001 00 1 00000 000001 02 2 000 1 00000 001 1 00 00001 0 1 1 0000 1 00000 171 Covance 6329-183 3M T-6295 PAGE: 2 DAYS 1-736 CATEGORY KEYWORD QUALIFIER WART LIKE LESION(S) SHOULDER-LEFT SHOULDER-RIGHT TAIL TAIL-MID TAIL-PROXIMAL VENTRAL-ABDOMINAL VENTRAL-ABDOMINAL-LEFT VENTRAL-ABDOMINAL-RIGHT VENTRAL-THORACIC-LEFT Table 6 Summary of Mass Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS SEX: GROUP : DOSE: NUMBER: NUMBER OF ANIMALS AFFECTED ------------ MALE 1 23456 0 0.5 2 5 20 20 70 60 60 60 70 40 001 000 000001 000001 0001 00 000000 0001 01 000001 000001 001 001 Covance 6329-183 3M T-6295 PAGE: 3 172 DAYS 1-736 CATEGORY KEYWORD QUALIFIER *** TOP OF LIST *** MASS(ES) AXILLARY REGION-LEFT AXILLARY REGION-RIGHT DORSAL DORSAL-CERVICAL-LEFT DORSAL-CERVICAL-RIGHT EAR-LEFT EAR-RIGHT HEAD-MAXILLARY-LEFT HEAD-MAXILLARY-RIGHT INGUINAL REGION-LEFT INGUINAL REGION-RIGHT LIMB-FRONT-LEFT LIMB-FRONT-RIGHT LIMB-HIND-LEFT LIMB-HIND-RIGHT MOUTH PAW-FRONT-LEFT PAW-HIND-RIGHT SHOULDER-LEFT SHOULDER-RIGHT TAIL TAIL-MID TAIL-PROXIMAL VENTRAL-ABDOMINAL Table 6 Summary of Mass Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS SEX: GROUP : DOSE: NUMBER: NUMBER OF ANIMALS AFFECTED ----------- FEMALE 123456 0 0.5 2 5 20 20 70 60 60 60 70 40 15 11 15 15 8 6 11 7 11 13 7 5 02 2 02 0 000000 000000 000000 000001 01 1 1 2 0 1 001 00 8 10 10 12 8 7 9 7 10 4 9 3 0001 00 000000 1 001 00 01 01 00 000000 000000 000000 0001 2 0 02 001 0 000000 000000 0001 00 12 7 6 5 4 1 173 Covance 6329-183 3M T-6295 PAGE: 4 DAYS 1-736 CATEGORY KEYWORD QUALIFIER MASS(ES) VENTRAL-ABDOMINAL-LEFT VENTRAL-ABDOMINAL-RIGHT VENTRAL-CERVICAL VENTRAL-CERVICAL-LEFT VENTRAL-CERVICAL-RIGHT VENTRAL-THORACIC VENTRAL-THORACIC-LEFT VENTRAL-THORACIC-RIGHT RECTUM WART LIKE LESION(S) AXILLARY REGION-LEFT AXILLARY REGION-RIGHT DORSAL DORSAL-CERVICAL DORSAL-CERVICAL-LEFT DORSAL-CERVICAL-RIGHT EAR-RIGHT HEAD-MAXILLARY-LEFT HEAD-MAXILLARY-RIGHT LIMB-FRONT-RIGHT MOUTH NOSE PAW-HIND-LEFT Table 6 Summary of Mass Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS SEX: GROUP : DOSE: NUMBER: NUMBER OF ANIMALS AFFECTED --- --FEMALE-- 12345 0 0.5 2 5 20 6 20 70 60 60 60 70 40 578994 11 10 8 6 8 5 23541 1 21 3530 04 1 5 00 2 01 02 0 32 002 1 1 1 01 1 2 3 00000 000000 000000 000000 001 000 000000 000000 000000 000001 000001 000000 4 0001 1 01 001 0 000000 174 Covance 6329-183 3M T-6295 PAGE: 5 DAYS 1-736 CATEGORY KEYWORD QUALIFIER WART LIKE LESION(S) SHOULDER-LEFT SHOULDER-RIGHT TAIL TAIL-MID TAIL-PROXIMAL VENTRAL-ABDOMINAL VENTRAL-ABDOMINAL-LEFT VENTRAL-ABDOMINAL-RIGHT VENTRAL-THORACIC-LEFT *** END OF LIST *** Table 6 Summary of Mass Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS SEX: GROUP : DOSE: NUMBER: NUMBER OF ANIMALS AFFECTED ----------- FEMALE 1 23456 0 0.5 2 5 20 20 70 60 60 60 70 40 000000 000000 001 000 000000 000001 001 000 000000 000000 000000 Covance 6329-183 3M T-6295 PAGE: 6 175 WEEK SEX: GROUP: DOSE: UNITS: 1 0 PPM 1N 70 MEAN 179 S.D. 18.6 2N 70 MEAN 237 S.D. 21.8 3N 70 MEAN 287 S.D. 25.0 4N 70 MEAN 334 S.D. 27.6 5N 65 MEAN 367 S.D. 31.4 6N 65 MEAN 399 S.D. 34.3 7N 65 MEAN 419 S.D. 35.1 Table 7 Summary of Body Weight Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS --------- MALE- 23456 0.5 2 5 20 20 PPM PPM PPM PPM PPM 60 178 18.7 60 237 20.2 60 290 21.4 60 333 23.9 55 370 27.1 55 402 30.8 55 428 33.3 60 179 19.0 60 237 21.5 60 286 23.0 60 328 25.4 55 367 28.4 55 397 30.3 55 418 31.8 60 179 19.2 60 236 20.5 60 290 22.8 60 333 24.3 55 367 27.9 55 399 30.4 55 425 32.2 70 178 18.7 70 234 20.2 70 285 23.2 70 323 25.0 65 361 28.1 65 390 30.2 65 413 32.7 40 178 19. 40 231 22. 40 286 25. 40 326 28. 40 363 31. 40 394 34. 40 415 36. Covance 6329-183 3M T-6295 PAGE: 1 176 Table 7 Summary of Body Weight Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS SEX: ---------------------------------- MALE GROUP: 1 2 3 4 5 6 DOSE: 0 0.5 2 5 20 20 WEEK UNITS: PPM PPM PPM PPM PPM PPM 8N 65 MEAN 448 S.D. 37.8 9N 65 MEAN 464 S.D. 41.6 10 N 65 MEAN 481 S.D. 43.1 11 N 65 MEAN 499 S.D. 45.2 12 N 65 MEAN 509 S.D. 46.8 13 N 65 MEAN 517 S.D. 48.0 14 N 65 MEAN 528 S.D. 51.1 55 450 35.7 55 463 37.7 55 483 39.8 55 502 42.1 55 512 43.9 55 518 45.6 55 534 47.3 55 443 35.9 55 450 38.2 55 473 40.7 55 479* 42.6 55 494 44.3 55 508 47.5 55 517 49.4 55 448 35.1 55 466 36.8 55 479 36.9 55 494 38.5 55 507 40.4 55 514 42.5 55 527 44.4 65 432 34.2 65 446* 36.4 65 465 37.2 65 474* 38.5 65 485* 41.5 65 493* 43.2 65 504* 46.1 40 436 40.8 40 446 41.0 40 466 42.2 40 473* 45.2 40 483* 46.4 40 491* 49.1 40 501* 50.4 Covance 6329-183 3M T-6295 PAGE: 2 177 Table 7 Summary of Body Weight Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS SEX: ---------------------------------- MALE GROUP: 1 2 3 4 5 6 DOSE: 0 0.5 2 5 20 20 WEEK UNITS: PPM PPM PPM PPM PPM PPM 15 N 60 MEAN 535 S.D. 51.9 16 N 60 MEAN 549 S.D. 53.5 17 N 60 MEAN 556 S.D. 55.4 21 N 60 MEAN 580 S.D. 62.1 25 N 59 MEAN 609 S.D. 67.8 29 N 59 MEAN 644 S.D. 76.0 33 N 59 MEAN 669 S.D. 79.5 50 542 48.2 50 553 49.9 50 559 50.5 49 594 57.5 49 621 63.0 49 654 66.6 49 683 71.0 50 531 49.0 50 542 50.0 50 551 51.0 50 582 57.5 50 611 61.0 50 640 67.4 50 666 70.4 50 535 45.5 50 550 46.5 50 557 47.8 50 581 53.2 50 604 56.7 50 632 62.5 50 653 68.8 60 513* 47.5 60 523* 49.3 60 526* 49.8 60 546* 57.2 60 567* 62.9 60 595* 69.0 60 619* 76.6 40 510* 51.2 40 517* 52.3 40 521* 52.9 40 544* 59.2 40 566* 65.8 40 595* 69.8 40 620* 75.1 Covance 6329-183 3M T-6295 PAGE: 3 178 Table 7 Summary of Body Weight Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS SEX: ---------------------------------- MALE GROUP: 1 2 3 4 5 6 DOSE: 0 0.5 2 5 20 20 WEEK UNITS: PPM PPM PPM PPM PPM PPM 37 N 59 MEAN 691 S.D. 83.5 41 N 59 MEAN 699 S.D. 87.9 45 N 59 MEAN 715 S.D. 95.8 49 N 58 MEAN 736 S.D. 97.2 53 N 58 MEAN 749 S.D. 103.5 57 N 48 MEAN 757 S.D. 109.7 61 N 47 MEAN 764 S.D. 122.9 49 700 77.8 48 716 81.8 48 735 87.8 48 759 89.5 48 770 91.7 47 781 95.6 46 790 97.0 50 687 73.8 50 702 78.2 50 721 80.4 50 740 83.5 50 753 86.2 48 761 88.0 48 777 91.3 50 676 73.6 50 690 77.6 50 709 82.3 50 726 88.9 48 742 97.1 47 753 100.2 47 768 106.4 59 644* 84.7 59 663 92.5 59 676 100.7 58 699 108.3 57 713 115.0 47 722 116.0 46 742 127.6 40 644* 81. 40 657 89. 40 674 97. 40 697 104. 40 712 109. 39 734 118. 39 742 123. Covance 6329-183 3M T-6295 PAGE: 4 179 Table 7 Summary of Body Weight Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS SEX: ---------------------------------- MALE GROUP: 1 2 3 4 5 6 DOSE: 0 0.5 2 5 20 20 WEEK UNITS: PPM PPM PPM PPM PPM PPM 65 N 44 MEAN 786 S.D. 115.1 69 N 43 MEAN 799 S.D. 120.1 73 N 41 MEAN 811 S.D. 113.7 77 N 40 MEAN 789 S.D. 124.1 81 N 31 MEAN 811 S.D. 124.9 85 N 27 MEAN 835 S.D. 115.7 89 N 25 MEAN 812 S.D. 137.1 45 795 104.4 45 791 108.1 44 790 116.1 42 772 114.2 37 774 111.7 32 762 114.7 26 760 125.1 48 783 94.7 48 784 105.6 47 790 115.1 45 773 119.1 39 779 132.6 36 781 138.8 31 795 129.9 46 782 109.5 45 794 113.0 44 804 115.4 44 799 125.0 40 799 124.4 37 772 117.3 36 759 136.4 44 765 123.9 44 772 127.4 43 779 145.1 41 783 142.4 38 785 121.9 37 790 128.7 35 796 136.1 39 740 133. 35 756 151. 31 801 145. 30 797 152. 29 788 153. 25 795 150. 24 782 140. Covance 6329-183 3M T-6295 PAGE: 5 180 WEEK SEX: GROUP: DOSE: UNITS: 1 0 PPM 93 N MEAN S.D. 97 N MEAN S.D. 101 N MEAN S.D. 105 N MEAN S.D. 22 825 128.2 19 772 132.2 15 755 159.1 11 746 164.5 Table 7 Summary of Body Weight Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS --------- MALE- 23456 0.5 2 5 20 20 PPM PPM PPM PPM PPM 22 786 109.6 19 761 105.3 17 717 123.5 11 734 154.1 29 809 123.8 23 789 157.6 19 814 138.3 17 809 129.6 27 821 112.4 27 814 116.2 26 802 124.6 25 768 130.4 35 799 129.9 32 769 140.8 27 765 122.3 23 755 136.1 21 767 133. 18 737 155. 14 754 127. 11 719 151. Covance 6329-183 3M T-6295 PAGE: 6 181 Table 7 Summary of Body Weight Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS WEEK SEX: --------------------------------- FEMALE 1 2GROUP: 3 0 2DOSE: 0.5 UNITS: PPM PPM PPM 4 5 PPM 1N 70 MEAN S.D. 15141.1 2N 70 MEAN 183 S.D. 14.6 3N 70 MEAN 207 S.D. 18.6 4N 70 MEAN S.D. 22242.1 5N 65 MEAN 237 S.D. 23.6 6N 65 MEAN 248 S.D. 25.3 7N 65 MEAN 254 S.D. 27.7 60 154 10.4 60 180 15.0 60 204 17.4 2226201.2 55 236 22.9 55 250 25.0 55 257 27.1 60 15150.0 60 176 14.2 60 199* 15.7 60 217 18.9 55 23220.1 55 24251.6 55 25210.0 60 15140.1 60 180 13.2 60 204 15.5 60 218 19.1 55 23220.0 55 24241.1 55 250 21.7 205 PPM 70 15131.1 70 177 13.5 70 199* 16.2 70 215* 17.4 65 224* 17.4 65 233* 19.6 65 23260*.1 206 PPM 40 15151.8 40 178 14.8 40 198* 15.8 40 214 18.8 40 227 19.4 40 235* 19.8 40 240* 18.8 Covance 6329-183 3M T-6295 PAGE: 7 182 WEEK SEX: GROUP: DOSE: UNITS: 1 0 PPM 8N 65 MEAN 266 S.D. 30.0 9N 65 MEAN 268 S.D. 31.4 10 N 65 MEAN 279 S.D. 33.5 11 N 65 MEAN 281 S.D. 33.8 12 N 65 MEAN 290 S.D. 37.0 13 N 65 MEAN 295 S.D. 38.9 14 N 65 MEAN 300 S.D. 40.1 Table 7 Summary of Body Weight Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS -------- FEMALE 23456 0.5 2 5 20 20 PPM PPM PPM PPM PPM 55 269 28.1 55 273 30.2 55 283 31.7 55 291 33.1 55 292 34.5 55 296 35.3 55 303 37.6 55 262 23.0 55 263 21.7 54 271 23.7 54 277 23.8 54 278 23.6 54 284 24.4 54 292 24.5 55 263 24.4 55 264 25.3 55 275 27.2 55 277 25.8 55 279 27.5 55 285 29.1 55 292 31.7 65 247* 20.2 65 245* 22.0 65 253* 22.4 65 258* 22.6 65 257* 21.1 65 261* 22.7 65 265* 23.3 40 250* 21. 40 247* 22. 40 254* 22. 40 255* 22. 40 259* 23. 40 262* 23. 40 266* 23. Covance 6329-183 3M T-6295 PAGE: 8 183 Table 7 Summary of Body Weight Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS SEX: --------------------------------- FEMALE GROUP: 1 2 3 4 5 6 DOSE: 0 0.5 2 5 20 20 WEEK UNITS: PPM PPM PPM PPM PPM PPM 15 N 60 MEAN 302 S.D. 42.2 16 N 60 MEAN 307 S.D. 43.9 17 N 60 MEAN 307 S.D. 43.9 21 N 60 MEAN 316 S.D. 44.8 25 N 60 MEAN 331 S.D. 50.9 29 N 60 MEAN 342 S.D. 52.8 33 N 60 MEAN 358 S.D. 58.0 50 304 36.7 50 310 39.1 50 311 38.3 50 322 40.2 50 335 45.6 50 347 50.0 50 362 55.0 49 294 26.4 49 298 28.2 49 303 26.5 49 314 29.9 49 324 30.7 49 338 36.1 49 356 40.7 50 298 33.3 50 303 33.8 50 305 34.9 50 313 38.2 50 328 42.2 50 342 47.2 49 353 46.8 60 266* 22.0 60 268* 23.1 60 272* 23.6 60 280* 24.1 60 290* 27.8 60 298* 30.2 60 310* 32.8 40 269* 23. 40 271* 24. 40 273* 25. 40 280* 26. 40 291* 29. 40 301* 32. 40 312* 36. Covance 6329-183 3M T-6295 PAGE: 9 184 WEEK SEX: GROUP: DOSE: UNITS: 1 0 PPM 37 N 60 MEAN 373 S.D. 64.8 41 N 60 MEAN 389 S.D. 69.4 45 N 60 MEAN 409 S.D. 73.3 49 N 60 MEAN 422 S.D. 74.5 53 N 60 MEAN 427 S.D. 78.8 57 N 50 MEAN 440 S.D. 79.8 61 N 48 MEAN 463 S.D. 81.3 Table 7 Summary of Body Weight Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS -------- FEMALE 23456 0.5 2 5 20 20 PPM PPM PPM PPM PPM 49 375 56.7 49 393 62.4 48 413 63.6 48 424 66.3 47 435 71.8 47 451 77.9 47 464 83.4 49 371 43.4 49 385 50.0 49 396 59.8 47 415 55.7 47 428 61.2 47 447 68.4 47 463 75.4 49 365 47.3 49 378 49.8 49 394 51.1 49 407 55.1 48 422 56.5 48 443 59.0 48 458 59.3 60 319* 36.6 59 328* 38.3 59 339* 41.0 59 351* 45.5 59 358* 48.3 48 371* 54.6 47 382* 58.8 40 321* 39. 40 329* 46. 39 346* 48. 39 356* 50. 39 366* 52. 39 393* 59. 39 419* 63. Covance 6329-183 3M T-6295 PAGE: 10 185 Table 7 Summary of Body Weight Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS SEX: --------------------------------- FEMALE GROUP: 1 2 3 4 5 6 DOSE: 0 0.5 2 5 20 20 WEEK UNITS: PPM PPM PPM PPM PPM PPM 65 N 47 MEAN 472 S.D. 86.7 69 N 47 MEAN 486 S.D. 92.2 73 N 45 MEAN 494 S.D. 98.0 77 N 44 MEAN 499 S.D. 103.3 81 N 41 MEAN 514 S.D. 100.3 85 N 37 MEAN 512 S.D. 107.0 89 N 32 MEAN 516 S.D. 89.6 46 473 93.5 43 496 93.6 39 508 89.0 39 519 93.7 39 526 100.9 37 537 98.0 34 550 110.6 46 469 85.5 42 492 85.2 39 504 93.6 37 509 104.3 35 503 118.8 26 525 128.8 23 523 120.9 48 458 63.6 47 475 66.0 45 478 78.0 44 477 78.3 39 485 79.8 37 493 83.4 37 496 81.3 46 384* 67.7 44 397* 63.0 44 400* 68.3 42 403* 78.3 40 417* 70.3 40 423* 76.1 38 431* 74.5 39 433 73. 38 460 76. 37 479 76. 35 486 79. 35 490 84. 32 500 98. 29 524 96. Covance 6329-183 3M T-6295 PAGE: 11 186 WEEK SEX: GROUP: DOSE: UNITS: 1 0 PPM 93 N MEAN S.D. 97 N MEAN S.D. 101 N MEAN S.D. 105 N MEAN S.D. 31 504 99.4 26 524 93.1 26 528 97.2 24 516 105.7 Table 7 Summary of Body Weight Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS -------- FEMALE 23456 0.5 2 5 20 20 PPM PPM PPM PPM PPM 29 550 114.7 25 556 103.8 19 541 96.5 15 523 117.0 16 565 125.9 14 572 139.3 12 566 151.0 0 33 509 72.3 25 518 77.9 19 529 78.9 17 518 85.6 33 438* 77.5 30 451* 85.9 27 452* 87.3 26 447 101.2 23 548 92. 22 555 97. 21 536 116. 19 542 138. Covance 6329-183 3M T-6295 PAGE: 12 187 Table 8 Summary of Body Weight Change Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS SEX: ---------------------------------- MALE GROUP: 1 2 3 4 5 6 DOSE: 0 0.5 2 5 20 20 WEEK UNITS: PPM PPM PPM PPM PPM PPM 1-2 N MEAN S.D. 2-3 N MEAN S.D. 3-4 N MEAN S.D. 4-5 N MEAN S.D. 5-6 N MEAN S.D. 6-7 N MEAN S.D. 7-8 N MEAN S.D. 70 58 5.8 70 50 6.5 70 47 6.8 65 34 7.6 65 32 6.2 65 20 6.5 65 29 5.8 60 59 5.6 60 53* 6.2 60 42* 8.0 55 38* 6.3 55 32 7.0 55 26* 5.9 55 22* 6.7 60 58 5.2 60 48 6.2 60 43* 7.6 55 38* 6.8 55 30 5.7 55 21 6.3 55 25* 7.2 60 57 4.4 60 55* 7.5 60 43* 6.8 55 34 8.2 55 32 5.3 55 26* 5.8 55 23* 6.7 70 57 5.9 70 50 6.2 70 38* 6.6 65 37 5.6 65 29 5.1 65 23 7.5 65 20* 5.5 40 53* 6.4 40 56* 7.2 40 40* 7.3 40 37 5.8 40 30 6.0 40 21 5.3 40 21* 10.4 Covance 6329-183 3M T-6295 PAGE: 1 188 Table 8 Summary of Body Weight Change Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS SEX: ---------------------------------- MALE GROUP: 1 2 3 4 5 6 DOSE: 0 0.5 2 5 20 20 WEEK UNITS: PPM PPM PPM PPM PPM PPM 8-9 N MEAN S.D. 9-10 N MEAN S.D. 10-11 N MEAN S.D. 11-12 N MEAN S.D. 12-13 N MEAN S.D. 13-14 N MEAN S.D. 14-15 N MEAN S.D. 65 17 11.0 65 16 5.7 65 18 5.3 65 10 8.2 65 9 5.8 65 11 5.4 60 7 5.8 55 13 6.1 55 20* 4.7 55 19 5.1 55 10 4.2 55 6* 5.5 55 16* 4.7 50 6 6.5 55 7* 5.8 55 23* 6.5 55 6* 6.9 55 15* 9.3 55 14* 6.8 55 9 4.4 50 9 4.7 55 18 4.7 55 13* 4.0 55 15* 6.8 55 13* 6.0 55 7 5.1 55 13 4.3 50 11* 5.7 65 13 7.9 65 20* 5.3 65 9* 6.5 65 10 5.6 65 8 4.7 65 11 7.1 60 8 4.5 40 9* 9.0 40 20* 5.0 40 8* 5.8 40 10 4.3 40 8 5.4 40 10 5.8 40 9 5.1 Covance 6329-183 3M T-6295 PAGE: 2 189 WEEK SEX: GROUP: DOSE: UNITS: 1 0 PPM 15-16 N MEAN S.D. 16-17 N MEAN S.D. 17-21 N MEAN S.D. 21-25 N MEAN S.D. 25-29 N MEAN S.D. 29-33 N MEAN S.D. 33-37 N MEAN S.D. 60 14 4.7 60 7 5.3 60 25 12.0 59 28 9.9 59 35 13.0 59 26 10.2 59 21 10.3 Table 8 Summary of Body Weight Change Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ---------MALE- 23456 0.5 2 5 20 20 PPM PPM PPM PPM PPM 50 11* 5.6 50 7 4.6 49 34* 10.5 49 27 12.9 49 34 13.5 49 29 10.7 49 17 23.1 50 11* 4.7 50 9 4.6 50 30* 11.3 50 30 10.5 50 29* 10.3 50 25 8.9 50 21 13.0 50 14 3.9 50 8 4.2 50 23 11.6 50 23 9.9 50 28* 9.6 50 20* 12.1 50 23 9.8 60 10* 4.6 60 3* 5.5 60 20 11.4 60 21* 9.9 60 28* 11.7 60 24 12.4 59 26 11.9 40 8* 4. 40 4* 4. 40 22 10. 40 22* 12. 40 30 7. 40 25 9. 40 24 11. Covance 6329-183 3M T-6295 PAGE: 3 190 Table 8 Summary of Body Weight Change Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS SEX: ---------------------------------- MALE GROUP: 1 2 3 4 5 6 DOSE: 0 0.5 2 5 20 20 WEEK UNITS: PPM PPM PPM PPM PPM PPM 37-41 N MEAN S.D. 41-45 N MEAN S.D. 45-49 N MEAN S.D. 49-53 N MEAN S.D. 53-57 N MEAN S.D. 57-61 N MEAN S.D. 61-65 N MEAN S.D. 59 8 12.5 59 16 19.8 58 20 15.6 58 13 15.9 48 10 20.8 47 8 37.4 44 6 25.5 48 13 11.9 48 19 10.2 48 24 8.9 48 11 15.0 47 12 14.5 46 4 26.6 45 6 18.3 50 15* 10.6 50 19 8.6 50 19 9.7 50 13 8.9 48 9 9.7 48 16 15.0 48 7 18.7 50 15* 14.2 50 19 12.4 50 17 12.9 48 12 28.3 47 7 13.1 47 15 22.9 46 12 23.9 59 19* 11.7 59 13 14.1 58 21 13.8 57 14 13.2 47 13 21.4 46 16 21.1 44 14 9.3 40 13 11. 40 16 10. 40 24 9. 40 15 10. 39 22* 17. 39 8 32. 39 -2 60. Covance 6329-183 3M T-6295 PAGE: 4 191 Table 8 Summary of Body Weight Change Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS SEX: ---------------------------------- MALE GROUP: 1 2 3 4 5 6 DOSE: 0 0.5 2 5 20 20 WEEK UNITS: PPM PPM PPM PPM PPM PPM 65-69 N MEAN S.D. 69-73 N MEAN S.D. 73-77 N MEAN S.D. 77-81 N MEAN S.D. 81-85 N MEAN S.D. 85-89 N MEAN S.D. 89-93 N MEAN S.D. 43 10 21.8 41 6 17.8 40 -14 47.0 31 -8 34.0 27 -1 30.9 25 -23 46.0 22 -1 26.4 45 -4 41.6 44 -6 46.7 42 -32* 58.0 37 -18 43.3 32 -24 62.6 26 -14 48.6 22 -1 30.2 48 1 37.2 47 0 34.4 45 -28* 53.4 39 -10 40.2 36 -14 52.7 31 -3* 29.5 29 -7 43.7 45 10 19.4 44 8 19.2 44 -5 19.0 40 -2 31.0 37 -13 45.0 36 -17* 59.8 27 15 13.3 44 7 25.4 43 6* 49.1 41 -7 33.0 38 -8 32.9 37 5 32.6 35 3* 23.6 35 3 48.7 35 3 43.2 31 19* 25.4 30 -8 33.8 29 -3 45.3 25 -15 36.3 24 -21 39.0 21 -2 52.7 Covance 6329-183 3M T-6295 PAGE: 5 192 WEEK SEX: GROUP: DOSE: UNITS: 1 0 PPM 93-97 N MEAN S.D. 97-101 N MEAN S.D. 01-105 N MEAN S.D. 1-15 N MEAN S.D. 15-29 N MEAN S.D. 29-57 N MEAN S.D. 57-105 N MEAN S.D. 1-105 N MEAN S.D. 19 -28 58.1 15 -29 47.9 11 -21 44.8 60 356 45.5 59 109 31.4 48 114 53.7 11 13 123.1 11 562 157.0 Table 8 Summary of Body Weight Change Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ---------MALE- 23456 0.5 2 5 20 20 PPM PPM PPM PPM PPM 19 -33 51.8 17 -55 85.0 11 -30 68.4 50 364 46.3 49 112 25.5 47 128 42.5 11 2 170.9 11 560 160.7 23 -10 42.4 19 -6 41.4 17 -23 44.5 50 352 44.0 50 109 24.8 48 120 32.0 17 70 80.5 17 631 128.1 27 -6 31.1 26 -10 32.7 25 -33 64.6 50 356 42.0 50 97 23.5 47 119 57.5 25 39 100.6 25 588 128.3 32 -17 55.1 27 -13 52.5 23 -21 41.4 60 334* 44.0 60 82* 30.8 47 131 62.9 23 70 106.5 23 580 137.6 18 -31 71. 14 -32 42. 11 -39 64. 40 332* 47. 40 86* 23. 39 138 55. 11 9 116. 11 546 147. Covance 6329-183 3M T-6295 PAGE: 6 193 Table 8 Summary of Body Weight Change Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS SEX: --------------------------------- FEMALE GROUP: 1 2 3 4 5 6 DOSE: 0 0.5 2 5 20 20 WEEK UNITS: PPM PPM PPM PPM PPM PPM 1-2 N MEAN S.D. 2-3 N MEAN S.D. 3-4 N MEAN S.D. 4-5 N MEAN S.D. 5-6 N MEAN S.D. 6-7 N MEAN S.D. 7-8 N MEAN S.D. 70 29 6.7 70 24 7.0 70 17 7.8 65 14 7.2 65 11 6.5 65 6 5.8 65 13 6.8 60 26* 7.5 60 24 6.6 60 18 7.0 55 14 7.9 55 14 6.3 55 6 7.4 55 12 5.0 60 21* 6.9 60 23 6.3 60 18 6.9 55 15 8.2 55 13 5.4 55 6 5.9 55 11 7.4 60 26* 6.3 60 24 6.5 60 14 7.3 55 15 7.8 55 12 6.9 55 6 7.3 55 13 6.0 70 25* 7.2 70 22 7.0 70 16 7.6 65 9* 7.4 65 9 5.7 65 3* 5.0 65 11 4.9 40 23* 6.1 40 20* 7.4 40 16 8.2 40 13 7.2 40 8* 5.8 40 5 6.0 40 10 5.1 Covance 6329-183 3M T-6295 PAGE: 7 194 Table 8 Summary of Body Weight Change Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS SEX: --------------------------------- FEMALE 1 2GROUP: 3 0 2DOSE: 0.5 4 5 205 206 WEEK UNITS: PPM PPM PPM PPM PPM PPM 8-9 N MEAN S.D. 9-10 10-11 11-12 N MEAN S.D. N MEAN S.D. N MEAN S.D. 12-13 N MEAN S.D. 13-14 N MEAN S.D. 14-15 N MEAN S.D. 6526.0 6151 6.3 652 5.1 65 9 6.3 65 5 7.0 65 5 7.0 60 4 5.8 55 46.2 5105 6.8 558* 5.7 5516*.6 55 4 5.3 55 7 5.3 50 3 6.5 552 4.9 548* 5.8 546* 5.8 541* 5.7 54 7 5.9 548* 5.1 492 6.0 5516.2 5151 6.8 552 5.7 552* 6.0 556 4.8 55 7 5.5 50 4 6.4 65 -2* 4.6 658* 6.6 65 5* 5.6 65 -1* 5.8 65 3 5.3 65 4 5.1 601 5.4 40 -3* 4.6 40 7* 5.5 400 4.9 40 4* 4.6 40 3 5.0 40 5 4.3 402 3.8 Covance 6329-183 3M T-6295 PAGE: 8 195 Table 8 Summary of Body Weight Change Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS SEX: --------------------------------- FEMALE GROUP: 1 2 3 4 5 6 DOSE: 0 0.5 2 5 20 20 WEEK UNITS: PPM PPM PPM PPM PPM PPM 15-16 N MEAN S.D. 16-17 N MEAN S.D. 17-21 N MEAN S.D. 21-25 N MEAN S.D. 25-29 N MEAN S.D. 29-33 N MEAN S.D. 33-37 N MEAN S.D. 60 4 4.4 60 0 6.5 60 9 8.1 60 15 9.2 60 12 7.8 60 16 10.8 60 15 12.9 50 6 5.3 50 1 4.8 50 11 6.7 50 13 9.9 50 12 9.8 50 15 13.1 49 15 12.2 49 4 5.7 49 4* 7.0 49 11 10.6 49 10* 11.3 49 15 11.9 49 18 13.8 49 14 9.9 50 5 5.6 50 3 6.2 50 7 8.9 50 16 9.3 50 14 9.9 49 13 10.0 49 12 9.9 60 3 5.3 60 4* 4.5 60 8 6.6 60 9* 9.0 60 9 8.0 60 12 9.7 60 9* 8.2 40 3 4. 40 2 5. 40 7 6. 40 11 5. 40 9 7. 40 12 9. 40 9 10. Covance 6329-183 3M T-6295 PAGE: 9 196 Table 8 Summary of Body Weight Change Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS SEX: --------------------------------- FEMALE GROUP: 1 2 3 4 5 6 DOSE: 0 0.5 2 5 20 20 WEEK UNITS: PPM PPM PPM PPM PPM PPM 37-41 N MEAN S.D. 41-45 N MEAN S.D. 45-49 N MEAN S.D. 49-53 N MEAN S.D. 53-57 N MEAN S.D. 57-61 N MEAN S.D. 61-65 N MEAN S.D. 60 16 10.5 60 19 9.8 60 13 16.3 60 5 20.8 50 18 13.4 48 17 10.5 47 8 9.6 49 18 13.7 48 18 13.0 48 11 9.0 47 11* 10.6 47 16 17.4 47 12 14.8 46 9 25.2 49 14 18.0 49 11 33.2 47 13 8.8 47 13* 11.1 47 19 17.8 47 16 18.6 46 4 26.2 49 13 8.9 49 17 12.4 49 13 11.6 48 14* 10.4 48 21 11.3 48 15 12.4 48 -1 21.3 59 10* 9.3 59 11* 7.5 59 12 9.3 59 7 10.5 48 14 13.2 47 9* 13.4 46 5 19.5 40 8* 16.7 39 14* 10.1 39 10 8.9 39 10 7.3 39 27* 12.9 39 26* 11.0 39 14 19.3 Covance 6329-183 3M T-6295 PAGE: 10 197 Table 8 Summary of Body Weight Change Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS SEX: --------------------------------- FEMALE GROUP: 1 2 3 4 5 6 DOSE: 0 0.5 2 5 20 20 WEEK UNITS: PPM PPM PPM PPM PPM PPM 65-69 N MEAN S.D. 69-73 N MEAN S.D. 73-77 N MEAN S.D. 77-81 N MEAN S.D. 81-85 N MEAN S.D. 85-89 N MEAN S.D. 89-93 N MEAN S.D. 47 14 12.8 45 9 20.2 44 3 23.1 41 13 41.8 37 3 19.0 32 3 31.4 31 -12 41.6 43 14 19.0 39 10 14.3 39 11 14.5 39 7 33.6 37 3 31.3 34 8 40.5 29 -12 35.3 42 13 23.7 39 7 30.1 37 1 29.6 35 -10 37.5 26 -7 31.3 23 -8 39.0 16 -1 30.5 47 15 19.9 45 0 27.8 44 -5 35.7 39 2 25.8 37 3 23.4 37 3 27.6 33 5 27.5 44 14 9.6 44 2 27.0 42 2 22.7 40 5 21.2 40 6 16.6 38 4 17.1 33 5 23.5 38 24* 9.0 37 20* 10.0 35 10 15.3 35 4 24.7 32 8 33.6 29 9 22.5 23 14* 19.4 Covance 6329-183 3M T-6295 PAGE: 11 198 WEEK SEX: GROUP: DOSE: UNITS: 1 0 PPM 93-97 N MEAN S.D. 97-101 N MEAN S.D. 01-105 N MEAN S.D. 1-15 N MEAN S.D. 15-29 N MEAN S.D. 29-57 N MEAN S.D. 57-105 N MEAN S.D. 1-105 N MEAN S.D. 26 -1 21.1 26 4 30.5 24 -16 27.6 60 149 35.2 50 100 56.5 60 38 16.2 24 84 62.7 24 363 103.9 Table 8 Summary of Body Weight Change Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS --------FEMALE 23456 0.5 2 5 20 20 PPM PPM PPM PPM PPM 25 -14 45.6 19 -23 48.2 15 -26 57.1 50 151 30.8 47 107 42.7 50 43 20.3 15 81 102.9 15 372 114.7 14 0 26.6 12 -6 20.9 0 49 140 20.5 47 111 48.4 49 44 18.6 0 0 25 2 38.8 19 -7 19.8 17 -11 44.1 50 144 28.2 48 102 30.7 50 44 20.2 17 98 66.9 17 366 82.4 30 10 23.9 27 0 22.4 26 -4 45.3 60 114* 18.6 48 74 30.8 60 33* 13.7 26 83 85.2 26 295 100.8 22 4 37. 21 -15 47. 19 -5 39. 40 114* 17. 39 92 36. 40 32 16. 19 162* 117. 19 386 133. Covance 6329-183 3M T-6295 PAGE: 12 199 Table 9 Summary of Food Consumption Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS SEX: ---------------------------------- MALE GROUP: 1 2 3 4 5 6 DOSE: 0 0.5 2 5 20 20 WEEK UNITS: PPM PPM PPM PPM PPM PPM 1N 69 MEAN 167 S.D. 13.3 2N 62 MEAN 177 S.D. 13.9 3N 68 MEAN 184 S.D. 12.9 4N 60 MEAN 191 S.D. 16.5 5N 62 MEAN 191 S.D. 16.1 6N 56 MEAN 192 S.D. 15.1 7N 59 MEAN 193 S.D. 15.6 56 165 11.5 54 173 12.9 52 183 13.0 47 188 13.7 50 193 16.1 43 191 17.3 42 195 18.3 60 165 14.1 58 173 13.9 56 181 14.1 49 184 19.3 50 186 16.7 46 187 15.9 47 183* 18.8 59 164 13.2 54 172 13.4 57 182 13.4 48 177* 15.0 49 185 16.3 49 186 16.4 45 187 17.2 69 158* 11.6 69 167* 13.8 70 178 15.2 62 176* 14.7 64 183* 15.6 65 181* 15.9 60 183* 15.4 39 166 14. 37 172 17. 38 181 16. 38 184 17. 40 187 18. 38 185 17. 37 184 19. Covance 6329-183 3M T-6295 PAGE: 1 200 Table 9 Summary of Food Consumption Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS SEX: ---------------------------------- MALE GROUP: 1 2 3 4 5 6 DOSE: 0 0.5 2 5 20 20 WEEK UNITS: PPM PPM PPM PPM PPM PPM 8N 54 MEAN 189 S.D. 19.2 9N 59 MEAN 190 S.D. 18.0 10 N 59 MEAN 188 S.D. 18.0 11 N 60 MEAN 188 S.D. 16.8 12 N 57 MEAN 189 S.D. 20.1 13 N 60 MEAN 185 S.D. 19.2 14 N 59 MEAN 184 S.D. 17.4 45 193 17.9 47 190 16.9 48 189 17.6 46 189 18.3 51 189 18.5 52 187 17.4 49 186 19.0 47 188 17.7 51 183 17.4 46 188 17.2 48 183 17.7 52 184 20.5 54 187 16.8 48 188 18.9 43 185 15.3 46 190 15.5 48 183 15.6 45 185 15.7 49 185 16.7 52 185 18.8 45 182 17.0 60 178* 18.0 60 175* 17.0 59 177* 15.1 59 179* 17.8 61 180 21.1 63 180 19.6 57 178 16.0 34 181 19.7 32 182 17.8 38 185 19.1 38 182 18.9 39 178* 18.6 37 178 21.0 38 184 20.8 Covance 6329-183 3M T-6295 PAGE: 2 201 Table 9 Summary of Food Consumption Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS SEX: ---------------------------------- MALE GROUP: 1 2 3 4 5 6 DOSE: 0 0.5 2 5 20 20 WEEK UNITS: PPM PPM PPM PPM PPM PPM 15 N 58 MEAN 190 S.D. 16.7 16 N 57 MEAN 190 S.D. 16.5 20 N 58 MEAN 192 S.D. 19.7 24 N 58 MEAN 184 S.D. 20.1 28 N 56 MEAN 183 S.D. 20.4 32 N 58 MEAN 177 S.D. 16.8 36 N 59 MEAN 183 S.D. 17.9 47 188 18.8 49 191 17.3 46 195 17.9 48 188 20.1 46 185 15.6 45 184 14.4 48 181 28.3 47 191 16.0 48 192 14.0 49 194 16.1 49 192 18.6 48 185 17.5 48 184 15.3 50 181 16.6 45 187 14.7 49 191 19.5 47 188 20.9 50 184 20.2 46 181 16.3 47 182 17.4 50 183 19.9 56 182 17.1 59 183 17.6 57 184 18.3 60 178 20.3 59 180 18.7 59 182 19.3 58 183 21.1 40 185 18.7 38 183 18.6 38 184 21.3 39 178 21.5 36 180 17.8 35 179 18.7 39 181 23.0 Covance 6329-183 3M T-6295 PAGE: 3 202 Table 9 Summary of Food Consumption Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS SEX: ---------------------------------- MALE GROUP: 1 2 3 4 5 6 DOSE: 0 0.5 2 5 20 20 WEEK UNITS: PPM PPM PPM PPM PPM PPM 40 N 58 MEAN 184 S.D. 19.1 44 N 58 MEAN 182 S.D. 24.9 48 N 57 MEAN 184 S.D. 20.2 52 N 54 MEAN 176 S.D. 27.6 56 N 45 MEAN 189 S.D. 27.2 60 N 45 MEAN 185 S.D. 39.2 64 N 44 MEAN 190 S.D. 29.6 46 184 19.1 48 188 21.0 48 191 18.5 46 185 17.8 43 196 18.8 46 194 29.6 42 192 15.6 48 186 16.2 49 185 14.7 48 186 18.8 48 186 17.3 44 195 16.5 45 197 20.3 44 193 20.8 48 187 20.3 47 186 20.0 47 185 18.3 44 181 27.5 44 193 20.4 44 198 28.9 43 189 22.2 58 187 22.5 55 184 20.7 55 184 20.8 54 178 22.4 46 190 32.0 44 200 25.1 42 197 23.2 39 182 22. 39 188 23. 37 185 18. 37 179 20. 35 190 22. 37 194 32. 35 174 46. Covance 6329-183 3M T-6295 PAGE: 4 203 Table 9 Summary of Food Consumption Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS SEX: ---------------------------------- MALE GROUP: 1 2 3 4 5 6 DOSE: 0 0.5 2 5 20 20 WEEK UNITS: PPM PPM PPM PPM PPM PPM 68 N 43 MEAN 195 S.D. 25.8 72 N 40 MEAN 196 S.D. 25.4 76 N 38 MEAN 176 S.D. 42.9 80 N 31 MEAN 183 S.D. 45.0 84 N 27 MEAN 188 S.D. 30.1 N MEAN S.D. 25 184 43.4 92 N 21 MEAN 186 S.D. 23.3 43 187 41.2 43 180 43.4 41 170 47.5 36 168 35.9 31 172 43.5 24 175 43.8 21 184 26.0 46 194 33.0 45 192 30.1 43 176 52.1 36 187 35.0 32 183 48.9 29 189 29.6 26 185 36.7 42 201 37.8 43 197 24.5 38 193 26.4 37 191 20.3 36 186 33.9 34 169 64.4 24 198 16.0 43 199 26.7 43 193 43.7 38 190 33.7 37 189 35.0 34 196 34.3 35 199 26.8 34 185 42.8 29 186 38. 31 198 32. 30 188 39. 28 185 37. 24 187 35. 22 190 29. 18 180 42. Covance 6329-183 3M T-6295 PAGE: 5 CO CO 204 WEEK SEX: GROUP: DOSE: UNITS: 1 0 PPM 96 N MEAN S.D. 100 N MEAN S.D. 104 N MEAN S.D. 17 177 51.1 14 175 53.4 10 167 30.1 Table 9 Summary of Food Consumption Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS --------- MALE- 23456 0.5 2 5 20 20 PPM PPM PPM PPM PPM 19 167 35.7 14 166 46.8 10 158 59.5 21 186 47.0 17 197 34.1 14 185 45.0 25 194 20.1 24 188 29.7 23 169 53.6 30 183 46.0 27 188 37.0 22 173 50.1 15 158 48. 11 170 43. 10 155 53. Covance 6329-183 3M T-6295 PAGE: 6 205 Table 9 Summary of Food Consumption Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS SEX: --------------------------------- FEMALE GROUP: 1 2 3 4 5 6 DOSE: 0 0.5 2 5 20 20 WEEK UNITS: PPM PPM PPM PPM PPM PPM 1N 55 MEAN 129 S.D. 11.9 2N 62 MEAN 140 S.D. 16.9 3N 54 MEAN 144 S.D. 16.8 4N 47 MEAN 140 S.D. 15.9 5N 50 MEAN 146 S.D. 18.5 6N 45 MEAN 138 S.D. 18.6 7N 54 MEAN 138 S.D. 17.0 55 120* 12.0 48 133 16.5 49 142 17.0 44 137 17.5 48 146 17.6 40 142 23.3 49 142 17.1 48 123 14.3 51 131* 17.8 51 140 17.6 41 142 20.1 43 144 14.2 35 137 20.3 45 133 12.2 55 130 13.5 47 136 19.6 55 141 14.9 45 138 18.3 45 144 19.3 42 134 17.3 46 136 16.3 62 124 11.2 62 130* 13.8 57 131* 14.7 58 126* 12.5 55 135* 14.0 55 124* 11.4 53 126* 10.7 35 125 12. 34 126* 12. 33 129* 13. 33 128* 14. 33 130* 11. 31 124* 13. 36 123* 12. Covance 6329-183 3M T-6295 PAGE: 7 206 Table 9 Summary of Food Consumption Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS SEX: --------------------------------- FEMALE GROUP: 1 2 3 4 5 6 DOSE: 0 0.5 2 5 20 20 WEEK UNITS: PPM PPM PPM PPM PPM PPM 8N 46 MEAN 138 S.D. 18.5 9N 55 MEAN 140 S.D. 18.8 10 N 53 MEAN 141 S.D. 18.2 11 N 56 MEAN 138 S.D. 21.7 12 N 52 MEAN 138 S.D. 20.1 13 N 56 MEAN 133 S.D. 17.7 14 N 53 MEAN 131 S.D. 20.4 38 138 15.9 48 135 15.2 45 135 16.0 52 134 15.5 43 133 15.7 46 135 17.7 40 129 18.0 43 136 11.3 41 132 17.0 39 134 11.0 46 133 13.4 39 137 17.6 47 131 13.1 37 133 13.4 42 135 15.5 46 134 17.3 45 135 13.8 48 134 16.0 48 136 21.5 49 131 20.1 40 125 11.3 54 123* 14.7 56 122* 12.6 53 124* 10.0 55 122* 10.2 58 127* 18.8 59 122* 18.4 54 119* 11.3 31 121* 12. 36 125* 17. 34 125* 14. 38 125* 14. 35 123* 11. 35 125 16. 32 122* 8. Covance 6329-183 3M T-6295 PAGE: 8 207 Table 9 Summary of Food Consumption Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS SEX: --------------------------------- FEMALE 1 2GROUP: 3 0 2DOSE: 0.5 4 5 205 206 WEEK UNITS: PPM PPM PPM PPM PPM PPM 15 N MEAN S.D. 16 N MEAN S.D. 20 N MEAN S.D. 24 N MEAN S.D. 28 N MEAN S.D. 32 N MEAN S.D. 36 N MEAN S.D. 53 133 17.1 54 133 19.4 50 139 19.0 53 134 18.9 56 132 15.7 58 136 18.4 55 135 17.9 49 134 15.9 44 135 18.1 46 134 16.0 44 132 18.7 48 133 24.7 47 134 26.5 49 135 20.9 43 135 14.9 41 136 15.3 42 136 16.2 44 136 17.6 45 141 20.5 45 139 15.5 46 137 15.3 45 133 16.2 45 133 15.6 44 138 18.8 47 13220.0 48 132 18.7 45 134 17.9 47 133 17.8 57 124* 13.6 56 12142*.2 49 12182*.8 54 123* 14.0 15262* 20.4 58 124* 17.4 58 124* 16.1 39 125 17. 34 124 15. 28 131 14. 13222* 13. 14201* 18. 40 127 17. 39 123* 14. Covance 6329-183 3M T-6295 PAGE: 9 208 WEEK SEX: GROUP: DOSE: UNITS: 1 0 PPM 40 N 56 MEAN 139 S.D. 20.3 44 N 56 MEAN 137 S.D. 17.8 48 N 58 MEAN 134 S.D. 23.6 52 N 57 MEAN 133 S.D. 24.0 56 N 47 MEAN 147 S.D. 19.4 60 N 47 MEAN 146 S.D. 19.1 64 N 46 MEAN 146 S.D. 19.4 Table 9 Summary of Food Consumption Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS -------- FEMALE 23456 0.5 2 5 20 20 PPM PPM PPM PPM PPM 44 139 15.6 45 134 19.3 48 133 19.5 45 134 20.5 47 143 25.6 47 137 23.2 44 145 22.5 48 142 20.6 45 137 18.6 47 142 16.6 46 141 19.8 46 145 20.5 46 146 28.2 44 139 34.3 47 138 17.6 47 134 20.7 47 141 17.8 45 139 22.2 47 146 21.3 48 142 22.3 44 141 23.3 56 135 20.7 53 126* 17.1 57 133 20.0 57 133 21.9 47 133* 22.4 44 131* 19.2 40 143 39.1 38 130 14. 38 128 15. 38 130 13. 38 133 14. 35 142 18. 37 145 16. 37 144 22. Covance 6329-183 3M T-6295 PAGE: 10 209 WEEK SEX: GROUP: DOSE: UNITS: 1 0 PPM 68 N 47 MEAN 151 S.D. 20.2 72 N 44 MEAN 143 S.D. 25.4 76 N 44 MEAN 141 S.D. 29.4 80 N 41 MEAN 147 S.D. 26.0 84 N 37 MEAN 147 S.D. 26.0 N MEAN S.D. 30 142 25.6 92 N 26 MEAN 140 S.D. 29.1 Table 9 Summary of Food Consumption Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS -------- FEMALE 23456 0.5 2 5 20 20 PPM PPM PPM PPM PPM 43 150 25.5 38 146 21.7 39 152 22.0 37 148 34.9 35 150 31.7 34 144 38.4 26 139 35.0 39 149 28.6 35 146 25.9 35 146 29.9 33 128 46.9 26 141 34.0 22 132 40.7 15 148 38.5 46 149 23.1 42 141 29.3 42 133 41.4 38 144 29.3 36 148 26.5 36 144 24.3 31 136 32.4 42 143 21.4 41 133 32.4 40 136 27.2 39 147 26.2 37 147 27.1 35 145 21.4 32 138 26.5 37 158 15. 35 152 16. 35 150 18. 35 145 25. 31 152 28. 26 149 27. 21 156 18. Covance 6329-183 3M T-6295 PAGE: 11 CO CO 210 WEEK SEX: GROUP: DOSE: UNITS: 1 0 PPM 96 N MEAN S.D. 100 N MEAN S.D. 104 N MEAN S.D. 26 143 24.3 24 137 30.1 24 128 37.6 Table 9 Summary of Food Consumption Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS --------FEMALE 23456 0.5 2 5 20 20 PPM PPM PPM PPM PPM 22 131 40.9 17 115 48.8 10 118 40.5 13 145 36.7 12 148 33.9 0 23 135 43.8 16 141 28.5 17 145 48.4 28 141 29.6 25 139 32.5 23 133 39.9 17 141 40. 20 135 41. 18 141 40. Covance 6329-183 3M T-6295 PAGE: 12 211 WEEK SEX: GROUP: DOSE: UNITS: 2 0.5 PPM 1N 56 MEAN 0.057 S.D. 0.004 2N 54 MEAN 0.047 S.D. 0.003 3N 52 MEAN 0.042 S.D. 0.002 4N 47 MEAN 0.038 S.D. 0.002 5N 50 MEAN 0.036 S.D. 0.002 6N 43 MEAN 0.033 S.D. 0.002 7N 42 MEAN 0.032 S.D. 0.002 Table 10 Summary of Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 1 3 2 PPM ---MALE--4 5 PPM 5 20 PPM 6 20 PPM 2 0.5 PPM 3 2 PPM FEMALE--4 5 PPM 5 20 PPM 6 20 PPM 60 0.226 0.015 58 0.189 0.012 56 0.169 0.010 49 0.151 0.011 50 0.139 0.007 46 0.132 0.007 47 0.122 0.010 59 0.570 0.041 54 0.471 0.031 57 0.420 0.026 48 0.364 0.020 49 0.346 0.019 49 0.325 0.017 45 0.311 0.016 69 2.205 0.156 69 1.846 0.115 70 1.675 0.097 62 1.477 0.081 64 1.394 0.072 65 1.287 0.065 60 1.241 0.061 39 2.336 0.170 37 1.907 0.118 38 1.701 0.097 38 1.531 0.086 40 1.408 0.073 38 1.307 0.068 37 1.233 0.078 55 0.052 0.004 48 0.050 0.005 49 0.048 0.003 44 0.043 0.003 48 0.043 0.003 40 0.040 0.005 49 0.038 0.003 48 0.213 0.020 51 0.200 0.019 51 0.194 0.026 41 0.180 0.018 43 0.170 0.011 35 0.157 0.019 45 0.148 0.010 55 0.559 0.046 47 0.506 0.055 55 0.481 0.038 45 0.440 0.049 45 0.431 0.036 42 0.390 0.034 46 0.382 0.028 62 2.149 0.141 62 1.965 0.162 57 1.805 0.157 58 1.646 0.121 55 1.669 0.137 55 1.502 0.106 53 1.501 0.112 35 2.160 0.178 34 1.929 0.164 33 1.792 0.126 33 1.656 0.142 33 1.613 0.113 31 1.488 0.107 36 1.446 0.089 212 WEEK SEX: GROUP: DOSE: UNITS: 2 0.5 PPM 8N MEAN S.D. 9N MEAN S.D. 10 N MEAN S.D. 11 N MEAN S.D. 12 N MEAN S.D. 13 N MEAN S.D. 14 N MEAN S.D. 45 0.030 0.002 47 0.029 0.001 48 0.028 0.002 46 0.027 0.002 51 0.026 0.002 52 0.025 0.002 49 0.025 0.002 Table 10 Summary of Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 2 3 2 PPM ---- MALE--4 5 PPM 5 20 PPM 6 20 PPM 2 0.5 PPM 3 2 PPM --- FEMALE--4 5 PPM 5 20 PPM 6 20 PPM 47 0.121 0.007 51 0.113 0.006 46 0.113 0.006 48 0.108 0.007 52 0.106 0.009 54 0.105 0.006 48 0.102 0.007 43 0.293 0.012 46 0.288 0.016 48 0.270 0.017 45 0.266 0.014 49 0.262 0.016 52 0.256 0.017 45 0.248 0.018 60 1.161 0.093 60 1.108 0.076 59 1.074 0.060 59 1.056 0.060 61 1.055 0.101 63 1.029 0.093 57 1.007 0.073 34 1.178 0.106 32 1.135 0.063 38 1.130 0.080 38 1.086 0.071 39 1.050 0.075 37 1.024 0.080 38 1.043 0.104 38 0.036 0.002 48 0.035 0.002 45 0.033 0.002 52 0.033 0.003 43 0.032 0.003 46 0.032 0.003 40 0.030 0.004 43 0.148 0.011 41 0.143 0.023 39 0.138 0.010 46 0.136 0.011 39 0.137 0.015 47 0.129 0.011 37 0.130 0.012 42 0.368 0.030 46 0.355 0.027 45 0.355 0.033 48 0.346 0.029 48 0.344 0.040 49 0.324 0.026 40 0.310 0.031 54 1.438 0.133 56 1.407 0.120 53 1.393 0.101 55 1.361 0.101 58 1.406 0.193 59 1.330 0.194 54 1.289 0.118 31 1.392 0.099 36 1.422 0.202 34 1.407 0.111 38 1.393 0.120 35 1.363 0.126 35 1.355 0.130 32 1.313 0.101 213 WEEK SEX: GROUP: DOSE: UNITS: 2 0.5 PPM 15 N MEAN S.D. 16 N MEAN S.D. 20 N MEAN S.D. 24 N MEAN S.D. 28 N MEAN S.D. 32 N MEAN S.D. 36 N MEAN S.D. 47 0.025 0.002 49 0.025 0.002 46 0.024 0.001 48 0.022 0.002 46 0.021 0.002 45 0.019 0.001 48 0.019 0.003 Table 10 Summary of Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 3 3 2 PPM ---- MALE--4 5 PPM 5 20 PPM 6 20 PPM 2 0.5 PPM 3 2 PPM --- FEMALE--4 5 PPM 5 20 PPM 6 20 PPM 47 0.102 0.006 48 0.100 0.006 49 0.096 0.007 49 0.090 0.007 48 0.083 0.006 48 0.080 0.006 50 0.076 0.008 45 0.250 0.013 49 0.247 0.017 47 0.234 0.020 50 0.219 0.018 46 0.206 0.016 47 0.199 0.012 50 0.195 0.021 56 1.010 0.074 59 1.006 0.084 57 0.969 0.065 60 0.904 0.070 59 0.875 0.082 59 0.850 0.083 58 0.817 0.084 40 1.032 0.069 38 1.010 0.067 38 0.970 0.073 39 0.904 0.084 36 0.872 0.069 35 0.837 0.059 39 0.805 0.046 49 0.031 0.004 44 0.031 0.004 46 0.030 0.003 44 0.028 0.004 48 0.028 0.005 47 0.027 0.006 49 0.026 0.003 43 0.129 0.011 41 0.129 0.016 42 0.124 0.012 44 0.120 0.015 45 0.121 0.022 45 0.112 0.013 46 0.106 0.012 45 0.318 0.023 45 0.315 0.027 44 0.316 0.029 47 0.288 0.024 48 0.279 0.025 45 0.273 0.028 47 0.261 0.023 57 1.330 0.121 56 1.315 0.113 49 1.305 0.104 54 1.218 0.110 56 1.181 0.164 58 1.152 0.111 58 1.111 0.099 39 1.333 0.160 34 1.302 0.132 28 1.345 0.105 32 1.200 0.098 40 1.157 0.174 40 1.170 0.155 39 1.103 0.080 214 WEEK SEX: GROUP: DOSE: UNITS: 2 0.5 PPM 40 N MEAN S.D. 44 N MEAN S.D. 48 N MEAN S.D. 52 N MEAN S.D. 56 N MEAN S.D. 60 N MEAN S.D. 64 N MEAN S.D. 46 0.018 0.002 48 0.018 0.002 48 0.018 0.002 46 0.017 0.002 43 0.018 0.001 46 0.018 0.003 42 0.017 0.002 Table 10 Summary of Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 4 3 2 PPM ---- MALE--4 5 PPM 5 20 PPM 6 20 PPM 2 0.5 PPM 3 2 PPM --- FEMALE--4 5 PPM 5 20 PPM 6 20 PPM 48 0.076 0.005 49 0.074 0.006 48 0.072 0.007 48 0.071 0.006 44 0.073 0.007 45 0.073 0.008 44 0.071 0.010 48 0.196 0.020 47 0.188 0.017 47 0.184 0.017 44 0.174 0.025 44 0.183 0.016 44 0.183 0.025 43 0.173 0.017 58 0.812 0.090 55 0.786 0.069 55 0.765 0.080 54 0.714 0.082 46 0.750 0.099 44 0.783 0.076 42 0.740 0.071 39 0.794 0.052 39 0.801 0.055 37 0.780 0.049 37 0.732 0.053 35 0.000 0.000 37 0.000 0.000 35 0.000 0.000 44 0.025 0.003 45 0.024 0.003 48 0.023 0.003 45 0.022 0.003 47 0.023 0.004 47 0.021 0.003 44 0.022 0.003 48 0.106 0.014 45 0.098 0.012 47 0.099 0.013 46 0.095 0.012 46 0.095 0.014 46 0.090 0.017 44 0.085 0.018 47 0.263 0.025 47 0.244 0.027 47 0.247 0.020 45 0.237 0.028 47 0.238 0.029 48 0.223 0.026 44 0.222 0.031 56 1.179 0.132 53 1.069 0.112 57 1.100 0.137 57 1.070 0.125 47 1.036 0.127 44 0.994 0.123 40 1.094 0.403 38 1.122 0.092 38 1.065 0.128 38 1.054 0.119 38 1.047 0.097 35 0.000 0.000 37 0.000 0.000 37 0.000 0.000 215 WEEK SEX: GROUP: DOSE: UNITS: 2 0.5 PPM 68 N MEAN S.D. 72 N MEAN S.D. 76 N MEAN S.D. 80 N MEAN S.D. 84 N MEAN S.D. 88 N MEAN S.D. 92 N MEAN S.D. 43 0.017 0.004 43 0.016 0.004 41 0.015 0.004 36 0.016 0.003 31 0.016 0.004 24 0.016 0.003 21 0.017 0.002 Table 10 Summary of Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 5 3 2 PPM ---- MALE--4 5 PPM 5 20 PPM 6 20 PPM 2 0.5 PPM 3 2 PPM --- FEMALE--4 5 PPM 5 20 PPM 6 20 PPM 46 0.071 0.012 45 0.070 0.010 43 0.064 0.017 36 0.068 0.011 32 0.065 0.017 29 0.068 0.007 26 0.065 0.011 42 0.186 0.056 43 0.175 0.019 38 0.173 0.018 37 0.171 0.021 36 0.171 0.027 34 0.154 0.055 24 0.171 0.018 43 0.755 0.113 43 0.708 0.146 38 0.700 0.120 37 0.700 0.139 34 0.711 0.109 35 0.724 0.099 34 0.676 0.174 29 0.000 0.000 31 0.000 0.000 30 0.000 0.000 28 0.000 0.000 24 0.000 0.000 22 0.000 0.000 18 0.000 0.000 43 0.022 0.003 38 0.021 0.002 39 0.021 0.003 37 0.020 0.004 35 0.020 0.004 34 0.019 0.004 26 0.018 0.004 39 0.087 0.015 35 0.084 0.014 35 0.082 0.013 33 0.073 0.023 26 0.078 0.016 22 0.073 0.022 15 0.075 0.015 46 0.227 0.030 42 0.209 0.027 42 0.197 0.052 38 0.212 0.034 36 0.216 0.029 36 0.208 0.032 31 0.191 0.041 42 1.054 0.142 41 0.957 0.205 40 0.971 0.148 39 1.019 0.187 37 1.001 0.132 35 0.984 0.165 32 0.912 0.148 37 0.000 0.000 35 0.000 0.000 35 0.000 0.000 35 0.000 0.000 31 0.000 0.000 26 0.000 0.000 21 0.000 0.000 216 WEEK SEX: GROUP: DOSE: UNITS: 2 0.5 PPM 96 N MEAN S.D. 100 N MEAN S.D. 104 N MEAN S.D. 19 0.016 0.003 14 0.016 0.004 10 0.015 0.005 Table 10 Summary of Test Material Consumption Data (mg/kg/day) 104 -WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T- 6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 6 3 2 PPM --- MALE---4 5 PPM 5 20 PPM 6 20 PPM 2 0.5 PPM 3 2 PPM FEMALE--4 5 PPM 5 20 PPM 6 20 PPM 21 0.068 0.014 17 0.069 0.011 14 0.068 0.020 25 0.171 0.022 24 0.165 0.026 23 0.153 0.041 30 0.685 0.170 27 0.708 0.156 22 0.643 0.162 15 0.000 0.000 11 0.000 0.000 10 0.000 0.000 22 0.017 0.005 17 0.015 0.006 10 0.017 0.005 13 0 .075 0 .021 12 0 .077 0 .019 0 23 0.186 0.049 16 0.190 0.026 17 0.195 0.056 28 0.906 0.162 25 0.876 0.193 23 0.838 0.247 17 0.000 0.000 20 0.000 0.000 18 0.000 0.000 217 Table 11 Summary of Clinical Hematology Data Males Week 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS DOSE RBC HGB HCT MCV MCH MCHC PLT ppm T-6295 X106/^L G/DL % FL PG % X 103/m L 0 MEAN S.D. N 0.5 MEAN S.D. N 2.0 MEAN S.D. N 5.0 MEAN S.D. N 20.0 MEAN S.D. N 7.47 .459 10 7.22 .414 10 7.54 .599 10 7.43 .622 10 7.30 .234 10 15.2 .81 10 14.7 .77 10 15.3 1.09 10 15.4 1.04 10 15.0 .44 10 44.8 2.35 10 43.4 2.43 10 45.1 3.39 10 45.0 3.32 10 44.2 1.56 10 60.0 1.56 10 60.2 1.69 10 59.9 1.65 10 60.7 1.97 10 60.6 1.40 10 20.3 .73 10 20.4 .67 10 20.4 .85 10 20.7 .68 10 20.6 .45 10 33.9 .59 10 33.9 .36 10 34.0 .61 10 34.1 .41 10 33.9 .37 10 1048 107 10 1082 111 10 1028 68 10 1013 118 10 1051 118 10 Covance 6329-183 3M T-6295 218 Table 11 Summary of Clinical Hematology Data Males Week 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS DOSE WBC ppm T-6295 X103/ L N-SEG LYMPH X 103/m L X103/^L MONO EOSIN BASO N-SEG% X 103/m L X103/m L X 103/m L LYMPH% MONO% EOSIN% Covance 6329-183 3M T-6295 BASO% 0 MEAN 8.6 .7 7.2 .6 .1 .0 9 83 7 1 0 S.D. 2.03 .30 1.92 .16 .06 .00 3.5 5.3 2. 2 .5 0 N 10 10 10 10 10 10 10 10 10 10 10 0.5 MEAN 9.3 1.2 7.4 .6 .1 .0 12 82 6 1 0 S.D. 2.54 1.32 1.29 .31 .06 .00 7.6 9.1 2.5 .3 0 N 10 10 10 10 10 10 10 10 10 10 10 2.0 MEAN 9.3 .7 8.0 .6 .1 .0 7 86 6 1 0 S.D. 2.30 .23 1.94 .26 .06 .00 1.4 3.4 2.3 .5 0 N 10 10 10 10 10 10 10 10 10 10 10 5.0 MEAN 7.2 .6 6.1 .4 .1 .0 8 85 6 2 0 S.D. 1.15 .21 .99 .24 .15 .00 2.3 6.5 2.9 2.0 0 N 10 10 10 10 10 10 10 10 10 10 10 20.0 MEAN 8.4 .8 6.8 .6 .1 .0 10 83 6 1 0 S.D. 2.87 .46 2.19 .38 .07 .00 2.8 4.8 2.4 .4 0 N 10 10 10 10 10 10 10 10 10 10 10 219 Table 11 Summary of Clinical Hematology Data Females Week 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS DOSE RBC HGB HCT MCV MCH MCHC PLT ppm T-6295 X106/^L G/DL % FL PG % X 103/m L 0 MEAN S.D. N 0.5 MEAN S.D. N 2.0 MEAN S.D. N 5.0 MEAN S.D. N 20.0 MEAN S.D. N 7.28 .276 10 7.40 .367 10 7.49 .367 10 7.40 .396 10 7.38 .238 10 15.2 .45 10 15.3 .62 10 15.1 .70 10 15.4 .42 10 15.3 .27 10 45.1 1.20 10 45.7 1.46 10 45.2 1.64 10 45.8 1.00 10 45.6 1.07 10 62.0 1.80 10 61.8 1.36 10 60.4 1.62 10 62.0 2.25 10 61.9 1.21 10 20.8 .63 10 20.7 .58 10 20.2 .64 10 20.8 .79 10 20.8 .64 10 33.7 .41 10 33.5 .57 10 33.5 .55 10 33.6 .52 10 33.6 .53 10 1103 109.6 10 1126 181.3 10 1069 128.2 10 1104 116.6 10 1108 88.4 10 Covance 6329-183 3M T-6295 220 Table 11 Summary of Clinical Hematology Data Females Week 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS DOSE WBC ppm T-6295 X103/ L N-SEG LYMPH X103/m L X103/^L MONO EOSIN BASO N-SEG% X103/m L X103/m L X103/m L LYMPH% MONO% EOSIN% Covance 6329-183 3M T-6295 BASO% 0 MEAN S.D. N 7.4 2.1027 .7 1.023 26..132 10 ..230 10 .1 1.006 1..0000 9 103.5 84 104.0 25.2 10 2 101.4 0 10 0 0.5 MEAN S.D. N 61..051 10 .7 1.078 51..051 10 .3 1.013 .0 1.005 1..0000 111000.8 84 1100.4 4 101.7 101.6 0 10 0 2.0 MEAN 5.0 .4 4.3 .2 .0 .0 9 84 5 10 S.D. N 1.1080 1.015 1.1083 1.101 1.005 1.000 105.1 107.9 103.5 10.6 10 0 5.0 MEAN S.D. N 26..898 10 .5 6.0 1.023 2.1602 1..2101 .1 1.004 1..0000 1028.1 87 103.7 4 101.7 101.6 0 10 0 20.0 MEAN S.D. N 6.2 1.1080 .5 5.4 1.017 1.1072 .2 1.014 .1 1.004 1..0000 8 102.7 86 104.6 42.1 10 1 10 .7 0 10 0 221 Table 12 Summary of Clinical Hematology Data Males Week 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS DOSE RBC HGB HCT MCV MCH MCHC PLT ppm T-6295 X106/^L G/DL % FL PG % X 103/m L 0 MEAN S.D. N 0.5 MEAN S.D. N 2.0 MEAN S.D. N 5.0 MEAN S.D. N 20.0 MEAN S.D. N 8.55 .446 10 8.77 .441 10 8.68 .456 10 8.57 .478 10 8.23 .216 10 15.6 .69 10 15.8 .56 10 15.7 .60 10 15.2 .63 10 15.1 .51 10 45.9 1.83 10 46.7 1.45 10 46.5 1.65 10 45.2 1.61 10 44.6 1.30 10 53.7 1.52 10 53.3 1.68 10 53.6 2.19 10 52.8 1.64 10 54.2 1.42 10 18.3 .56 10 18.0 .48 10 18.1 .60 10 17.8 .48 10 18.4 .51 10 34.1 .55 10 33.9 .53 10 33.7 .47 10 33.7 .49 10 33.9 .38 10 1142 164 10 1092 104 10 1168 189 10 1078 155 10 1139 124 10 Covance 6329-183 3M T-6295 222 Table 12 Summary of Clinical Hematology Data Males Week 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS DOSE WBC ppm T 6295 X103/iL N-SEG LYMPH X103/iL X103/iL MONO EOSIN BASO N-SEG% X103/iL X103/iL X103/iL LYMPH% MONO% EOSIN% Covance 6329-183 3M T-6295 BASO% 0 MEAN 8.2 1.1 6.1 .8 .2 .0 14 74 10 2 0 S.D. 1.95 .37 1.46 .27 .07 .03 3.1 3.2 2 . 1 .6 .0 N 10 10 10 10 10 10 10 10 10 10 10 0.5 MEAN 8.5 1.3 6.2 .8 .2 .0 15 73 10 2 0 S.D. 1.20 .28 1.04 .19 .10 .00 3.6 4.5 1 . 6 1 . 1 .0 N 10 10 10 10 10 10 10 10 10 10 10 2.0 MEAN 7.8 1.2 5.7 .8 .2 .0 15 73 10 2 0 S.D. 1.64 .33 1.20 .29 .07 .03 1 . 8 3.8 2.8 1 . 1 .3 N 10 10 10 10 10 10 10 10 10 10 10 5.0 MEAN 7.8 1.2 5.7 .7 .2 .0 16 73 9 2 0 S.D. 2.08 .37 1.97 .20 .05 .00 4.5 6 . 1 2.5 .6 .0 N 10 10 10 10 10 10 10 10 10 10 10 20.0 MEAN 10.5 1.6 * 7.8 .9 .2 .0 16 73 8 2 0 S.D. 3.65 .35 3.43 .39 .08 .03 5.4 7.3 4.2 .7 .0 N 10 10 10 10 10 10 10 10 10 10 10 223 Table 12 Summary of Clinical Hematology Data Females Week 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS DOSE RBC HGB HCT MCV MCH MCHC PLT ppm T-6295 X106/^L G/DL % FL PG % X 103/m L 0 MEAN S.D. N 0.5 MEAN S.D. N 2.0 MEAN S.D. N 5.0 MEAN S.D. N 20.0 MEAN S.D. N 8.00 .535 10 8.22 .377 10 8.06 .253 10 7.97 .453 10 7.87 .247 10 15.3 .87 10 15.5 .59 10 15.2 .58 10 15.2 .70 10 15.1 .67 10 45.2 2.58 10 46.0 1.80 10 45.2 1.76 10 45.0 2.17 10 44.2 1.82 10 56.6 1.18 10 56.0 1.93 10 56.1 2.37 10 56.5 1.32 10 56.2 1.64 10 19.1 .25 10 18.9 .74 10 18.8 .72 10 19.0 .41 10 19.2 .59 10 33.8 .57 10 33.7 .91 10 33.6 .57 10 33.7 .65 10 34.1 1.08 10 1061 68.5 10 1143 67.8 10 1070 93.4 10 1082 64.7 10 1144 122.9 10 Covance 6329-183 3M T-6295 224 Table 12 Summary of Clinical Hematology Data Females Week 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS DOSE WBC ppm T 6295 X103/iL N-SEG LYMPH X103/iL X103/iL MONO EOSIN BASO N-SEG% X103/iL X103/iL X103/iL LYMPH% MONO% EOSIN% Covance 6329-183 3M T-6295 BASO% 0 MEAN 4.9 1.0 3.4 .4 .1 .0 20 69 8 2 0 S.D. 1.51 .53 1.14 .17 .07 .00 8.8 9.7 2.9 .9 .0 N 10 10 10 10 10 10 10 10 10 10 10 0.5 MEAN 6.4 1.0 4.8 .5 .1 .0 16 73 9 2 0 S.D. 2.64 .45 2.18 .25 .03 .00 5.1 9.1 4.0 .8 .0 N 10 10 10 10 10 10 10 10 10 10 10 2.0 MEAN 4.9 .7 3.9 .3 .1 .0 14 77 6 2 0 S.D. 1.65 .17 1.50 .08 .03 .00 4.4 5.1 1 . 2 .9 .0 N 10 10 10 10 10 10 10 10 10 10 10 5.0 MEAN 5.8 .9 4.4 .4 .1 .0 15 75 8 2 0 S.D. 1.96 .55 1.54 .32 .05 .03 7.1 7.7 3.9 .8 .4 N 10 10 10 10 10 10 10 10 10 10 10 20.0 MEAN 4.8 1.0 3.4 .3 .1 .0 19 72 7 2 0 S.D. 1.68 .64 1 . 1 2 .17 .06 .00 6.3 6.7 2.5 1.3 .3 N 10 10 10 10 10 10 10 10 10 10 10 225 Table 13 Summary of Clinical Hematology Data Males Week 27 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS DOSE RBC HGB HCT MCV MCH MCHC PLT ppm T-6295 X106/^L G/DL % FL PG % X 103/m L 0 MEAN S.D. N 0.5 MEAN S.D. N 2.0 MEAN S.D. N 5.0 MEAN S.D. N 20.0 MEAN S.D. N 8.78 .471 10 8.84 .629 10 8.82 .301 10 9.04 .439 10 8.67 .252 10 15.7 .58 10 15.5 .84 10 15.5 .54 10 15.8 .60 10 15.1 .62 10 46.1 1.96 10 46.0 2.34 10 45.6 1.62 10 46.6 1.84 10 44.7 1.22 10 52.6 1.60 10 52.1 1.63 10 51.8 1.01 10 51.6 1.40 10 51.6 2.06 10 17.9 .56 10 17.6 .60 10 17.5 .45 10 17.4 .58 10 17.4 .83 10 34.0 .34 10 33.7 .31 10 33.9 .38 10 33.8 .36 10 33.8 .57 10 1235 171 10 1270 138 10 1237 127 10 1217 116 10 1287 162 10 Covance 6329-183 3M T-6295 226 Table 13 Summary of Clinical Hematology Data Males Week 27 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS DOSE WBC ppm T-6295 X103/^L N-SEG X103/^L LYMPH X103/^L MONO EOSIN BASO X103/^L X103/^L X103/^L N-SEG% LYMPH% MONO% EOSIN% Covance 6329-183 3M T-6295 BASO% 0 MEAN 7.5 1.3 5.0 1.0 .2 .0 18 67 13 2 1 S.D. 1.43 .29 1.14 .23 .08 .05 4.1 3.9 2 . 1 1. 0 5 N 10 10 10 10 10 10 10 10 10 10 10 0.5 MEAN 8.2 1.5 5.6 .9 .2 .0 19 67 11 2 1 S.D. 1.42 .31 1.40 .19 .03 .05 5.6 6.2 2.3 .7 5 N 10 10 10 10 10 10 10 10 10 10 10 2.0 MEAN 8.7 1.7 5.7 1.0 .2 .0 19 66 12 2 0 S.D. 1.53 .52 1.28 .26 .07 .04 6 . 1 7.8 2. 2 .6 5 N 10 10 10 10 10 10 10 10 10 10 10 5.0 MEAN 8.6 1.6 5.7 1.2 .2 .0 19 65 14 2 1 S.D. 1.88 .31 1.74 .32 .05 .05 4.5 6.7 3.0 .4 5 N 10 10 10 10 10 10 10 10 10 10 10 20.0 MEAN 8.7 1.6 5.9 1.0 .1 .0 18 68 12 2 0 S.D. 1.88 .42 1.41 .28 .05 .04 3.2 4.9 2.6 .7 5 N 10 10 10 10 10 10 10 10 10 10 10 227 Table 13 Summary of Clinical Hematology Data Females Week 27 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS DOSE RBC HGB HCT MCV MCH MCHC PLT ppm T-6295 X106/^L G/DL % FL PG % X 103/m L 0 MEAN S.D. N 0.5 MEAN S.D. N 2.0 MEAN S.D. N 5.0 MEAN S.D. N 20.0 MEAN S.D. N 7.56 .563 10 7.93 .639 10 8.00 .312 10 8.12 .353 10 7.73 .398 10 14.6 .97 10 43.5 3.16 10 57.6 1.06 10 14.9 .52 10 44.8 1.92 10 56.8 2.94 10 15.1 .66 10 45.2 1.94 10 56.5 1.44 10 15.3 * .61 10 46.1 * 1.61 10 56.8 1.20 10 14.5 .53 10 43.3 1.70 10 56.1 1.87 10 19.2 .33 10 18.9 1.18 10 18.9 .53 10 18.9 .57 10 18.8 .66 10 33.4 .63 10 33.3 .35 10 33.4 .32 10 33.3 .44 10 33.5 .39 10 1051 121 10 1057 127 10 1074 75 10 1095 131 10 1062 153 10 Covance 6329-183 3M T-6295 228 Table 13 Summary of Clinical Hematology Data Females Week 27 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS DOSE WBC ppm T 6295 X103/iL N-SEG LYMPH X103/iL X103/iL MONO EOSIN BASO X103/iL X103/iL X103/iL N-SEG% LYMPH% MONO% EOSIN% Covance 6329-183 3M T-6295 BASO% 0 MEAN 5.5 .9 4.0 .6 .1 .0 16 72 10 2 0 S.D. .77 .54 .63 .26 .10 .03 9.1 9.9 3.3 1.3 .4 N 10 10 10 10 10 10 10 10 10 10 10 0.5 MEAN 6.7 1.1 5.0 .5 .1 .0 14 77 7 2 0 S.D. 4.82 1.22 3.24 .33 .05 .03 5.3 5.6 3.2 .6 .3 N 10 10 10 10 10 10 10 10 10 10 10 2.0 MEAN 4.8 .7 3.6 .4 .1 .0 15 74 8 2 0 S.D. 1.17 .21 .97 .18 .03 .00 3.1 6.0 3.4 1 . 1 .0 N 10 10 10 10 10 10 10 10 10 10 10 5.0 MEAN 6.1 .8 4.7 .5 .1 .0 14 76 8 2 0 S.D. 1.95 .23 1.74 .24 .05 .00 4.8 6.5 3.9 .6 .4 N 10 10 10 10 10 10 10 10 10 10 10 20.0 MEAN 5.9 .9 4.4 .4 .1 .0 16 75 7 2 0 S.D. 1.61 .55 1.41 .18 .04 .00 8.4 10.7 3.2 1. 0 .5 N 10 10 10 10 10 10 10 10 10 10 10 229 Table 14 Summary of Clinical Hematology Data Males Week 53 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS DOSE RBC HGB HCT MCV MCH MCHC PLT ppm T-6295 X106/^L G/DL % FL PG % X 103/m L 0 MEAN S.D. N 0.5 MEAN S.D. N 2.0 MEAN S.D. N 5.0 MEAN S.D. N 20.0 MEAN S.D. N 8.33 .286 10 8.33 .266 10 8.11 .288 10 8.43 .536 10 8.28 .360 9 15.5 .70 10 15.2 .21 10 14.9 .64 10 15.2 .45 10 14.9 .71 9 43.5 2.02 10 43.4 .77 10 42.5 1.63 10 43.7 1.56 10 41.7 1.74 9 52.3 1.70 10 52.1 1.23 10 52.4 1.79 10 52.0 2.41 10 50.4 1.58 9 18.6 .59 10 18.3 .57 10 18.4 .75 10 18.0 .94 10 17.9 .47 9 35.6 .38 10 1112 155 10 35.1 .49 10 1059 172 10 35.1 .36 10 931 108 10 34.7 * .58 10 1011 111 10 35.6 .49 9 1241 124 9 Covance 6329-183 3M T-6295 230 Table 14 Summary of Clinical Hematology Data Males Week 53 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS DOSE WBC ppm T 6295 X103/iL N-SEG LYMPH X103/iL X103/iL MONO EOSIN BASO X103/iL X103/iL X103/iL N-SEG% LYMPH% MONO% EOSIN% Covance 6329-183 3M T-6295 BASO% 0 MEAN 7.1 2.4 3.6 .9 .1 .0 34 51 13 2 0 S.D. 1.33 .96 .96 .26 .05 .00 11.9 10.6 3.2 .6 .4 N 10 10 10 10 10 10 10 10 10 10 10 0.5 MEAN 7.8 1.7 4.9 * 1.1 .1 .0 22 * 63 * 14 2 0 S.D. 1.59 .31 1 . 1 0 .32 .06 .03 3.0 4.0 2.5 .7 .4 N 10 10 10 10 10 10 10 10 10 10 10 2.0 MEAN 8.6 2.1 5.4 * 1.0 .1 .0 22 * 64 * 11 1 0 S.D. 2.97 1.64 1.20 .65 .04 .03 7.6 10.2 4.5 .5 .0 N 10 10 10 10 10 10 10 10 10 10 10 5.0 MEAN 8.5 2.0 5.4 * .9 .1 .0 24 63 * 11 2 0 S.D. 1.63 .74 1.28 .33 .04 .00 6.2 7.9 2.6 .5 .0 N 10 10 10 10 10 10 10 10 10 10 10 20.0 MEAN 7.3 2.3 4.0 .8 .1 .0 30 56 12 2 0 S.D. 1.78 1.58 1.29 .42 .03 .03 12.3 15.1 5.0 .5 .3 N 99999999999 231 Table 14 Summary of Clinical Hematology Data Females Week 53 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS DOSE RBC HGB HCT MCV MCH MCHC PLT 6295 X106/iL G/DL FL PG X 103/m L 0 MEAN S.D. N MEAN S.D. N MEAN S.D. N 5.0 MEAN S.D. N 20.0 MEAN S.D. N 7.39 .485 10 7.26 .695 10 7.31 .566 10 7.32 .479 10 7.05 .881 10 14.5 .76 10 14.2 1.04 10 14.3 .61 10 14.2 .56 10 13.5 1.66 10 40.5 1.84 10 41.0 3.11 10 41.1 1.91 10 41.1 1.73 10 38.1 4.56 10 54.9 1.64 10 56.6 2.40 10 56.3 2.32 10 56.2 1.96 10 54.0 1.25 10 19.6 .60 10 19.7 .94 10 19.7 .89 10 19.5 .83 10 19.2 .46 10 35.7 .31 10 34.7 * .42 10 34.9 * .45 10 34.7 * .40 10 1009 80 10 908 99 10 851 101 10 837 * 85 10 35.5 .29 10 1023 113 10 Covance 6329-183 3M T-6295 232 Table 14 Summary of Clinical Hematology Data Females Week 53 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS DOSE WBC ppm T 6295 X103/iL N-SEG LYMPH X103/iL X103/iL MONO EOSIN BASO X103/iL X103/iL X103/iL N-SEG% LYMPH% MONO% EOSIN% Covance 6329-183 3M T-6295 BASO% 0 MEAN 4.2 1.6 1.9 .5 .1 .0 37 49 12 2 0 S.D. 1.72 1.16 .57 .16 .00 .00 10.5 8.8 3.2 .8 .0 N 10 10 10 10 10 10 10 10 10 10 10 0.5 MEAN 4.7 1.0 3.1 * .5 .1 .0 22 * 64 * 12 2 0 S.D. 1.53 .28 1.27 .20 .03 .00 4.7 7.2 4.6 .8 .3 N 10 10 10 10 10 10 10 10 10 10 10 2.0 MEAN 5.2 1.5 3.1 * .5 .1 .0 28 59 10 3 0 S.D. 1.28 .54 .94 .16 .05 .00 7.4 8.6 2.5 .7 .0 N 10 10 10 10 10 10 10 10 10 10 10 5.0 MEAN 4.2 1.2 2.5 .5 .1 .0 27 60 11 2 0 S.D. 1.27 .58 .70 .19 .04 .00 8.2 8.2 3.9 .8 .3 N 10 10 10 10 10 10 10 10 10 10 10 20.0 MEAN 5.4 2.1 2.6 .5 .1 .0 34 53 10 2 0 S.D. 2.33 1.94 .90 .27 .04 .00 17.1 17.6 2.5 1.0 .4 N 10 10 10 10 10 10 10 10 10 10 10 233 Table 15 Summary of Clinical Chemistry Data Males Week 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS DOSE GLU ppm T-6295 MG/DL UN MG/DL CREAT MG/DL T PRO G/DL ALB G/DL GLOB G/DL T BILI MG/DL CHOL MG/DL 0 MEAN 97 12 .6 6.2 4.4 1 . 8 .1 49 S.D. 11.4 1 . 2 .18 .43 .26 .32 .09 66 N 10 10 10 10 10 10 10 10 0.5 MEAN 97 12 .6 6.4 4.6 1 . 8 .1 49 S.D. 5.2 1 . 8 .06 .33 .29 .31 .09 65 N 10 10 10 10 10 10 10 10 2.0 MEAN 91 12 .6 6.0 4.5 1 . 6 .1 44 S.D. 10.8 1.3 .14 .33 .29 .16 .08 86 N 10 10 10 10 10 10 10 10 5.0 MEAN 94 12 .6 6.3 4.6 1.7 .0 48 S.D. 8.9 2 . 1 .10 .29 .19 .15 .05 15 3 N 10 10 10 10 10 10 10 10 20.0 MEAN 84 * 13 .6 6.3 4.6 1.7 .1 45 S.D. 5.0 1.5 .12 .23 .18 .13 .09 13 7 N 10 10 10 10 10 10 10 10 Covance 6329-183 3M T-6295 234 Table 15 Summary of Clinical Chemistry Data Males Week 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS DOSE AST/SGOT ALT/SGPT ppm T--6295 IU/L IU/L GGT IU/L CA MG/DL I PHOS MG/DL NA MMOL/L K MMOL/L CL MMOL/L 0 MEAN S.D. N 0.5 MEAN S.D. N 2.0 MEAN S.D. N 5.0 MEAN S.D. N 20.0 MEAN S.D. N 122 26.4 10 146 28.9 10 104 22.6 10 114 17.2 10 131 19.9 10 31 3.4 10 32 4.2 10 28 5.2 10 30 3.8 10 33 3.0 10 1 1.3 10 0 .7 10 1 1.6 10 0 .7 10 1 1.3 10 10.7 .41 10 10.7 .31 10 10.6 .41 10 10.9 .28 10 10.6 .31 10 10.0 .54 10 9.5 .62 10 10.1 .39 10 9.8 .62 10 9.8 .59 10 148 1.6 10 147 1.3 10 148 1.4 10 147 1.4 10 148 1.4 10 6.3 .69 10 6.3 .42 10 6.2 .71 10 6.2 .62 10 6.1 .58 10 104 1.5 10 104 1.1 10 105 1.1 10 103 2.2 10 103 .9 10 Covance 6329-183 3M T-6295 235 Table 15 Summary of Clinical Chemistry Data Females Week 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS DOSE GLU ppm ' 6295 MG/DL UN MG/DL CREAT MG/DL T PRO G/DL ALB G/DL GLOB G/DL T BILI MG/DL CHOL MG/DL 0 MEAN 114 14 .7 6.9 5.1 1 . 8 .1 72 S.D. 7.1 1.7 .10 .14 . 1 1 .16 .10 11 N 10 10 10 10 10 10 10 10 0.5 MEAN 111 13 .8 6.8 5.0 1 . 8 .0 70 S.D. 7.0 1 . 8 .09 .16 .18 .23 .07 25 N 10 10 10 10 10 10 10 10 2.0 MEAN 113 14 .8 6.8 5.1 1.7 .0 61 S.D. 17.9 1 . 8 .13 .39 .32 .19 .07 17 N 10 10 10 10 10 10 10 10 5.0 MEAN 109 13 .7 7.1 5.3 1 . 8 .0 58 S.D. 1 1 . 0 1 . 2 .08 .35 .31 .17 .04 14 N 10 10 10 10 10 10 10 10 20.0 MEAN 107 14 .7 6.8 5.2 1 . 6 .0 54 S.D. 7.7 1 . 8 .10 .35 .19 .18 .10 10 N 10 10 10 10 10 10 10 10 Covance 6329-183 3M T-6295 236 Table 15 Summary of Clinical Chemistry Data Females Week 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS DOSE AST/SGOT ALT/SGPT ppm T--6295 IU/L IU/L GGT IU/L CA MG/DL I PHOS MG/DL NA MMOL/L K MMOL/L CL MMOL/L 0 MEAN S.D. N 0.5 MEAN S.D. N 2.0 MEAN S.D. N 5.0 MEAN S.D. N 20.0 MEAN S.D. N 123 28.2 10 120 37.2 10 101 12.2 10 112 23.9 10 92 * 16.0 10 32 4.8 10 31 5.6 10 29 5.6 10 32 5.1 10 32 7.1 10 0 .7 10 1 1.4 10 2 2.0 10 2 1.3 10 1 1.1 10 10.8 .34 10 10.6 .31 10 10.7 .37 10 10.7 .35 10 10.6 .24 10 8.5 .90 10 8.4 1.03 10 8.5 1.08 10 7.9 .83 10 8.2 1.47 10 145 1.0 10 145 .8 10 147 1.3 10 147 2.2 10 146 1.8 10 5.9 .43 10 6.1 .45 10 6.2 .51 10 6.1 .41 10 6.0 .56 10 104 1.3 10 104 .9 10 106 1.2 10 105 1.5 10 105 1.1 10 Covance 6329-183 3M T-6295 237 Table 15 Summary of Clinical Chemistry Data Males Week 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS DOSE PCOAO ppm T-6295 IU/G 0 MEAN S.D. N 0.5 MEAN S.D. N 2.0 MEAN S.D. N 5.0 MEAN S.D. N 20.0 MEAN S.D. N 9 2.2 5 9 2.3 5 7 4.0 5 8 .8 5 6 1.4 5 Covance 6329-183 3M T-6295 238 Table 15 Summary of Clinical Chemistry Data Females Week 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS DOSE PCOAO ppm T-6295 IU/G 0 MEAN S.D. N 0.5 MEAN S.D. N 2.0 MEAN S.D. N 5.0 MEAN S.D. N 20.0 MEAN S.D. N 5 1.5 5 6 1.1 5 3 1.7 5 2* 1.1 5 4 1.1 5 Covance 6329-183 3M T-6295 239 Table 16 Summary of Clinical Chemistry Data Males Week 14 (PCOAO) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS DOSE PCOAO ppm T-6295 IU/G 0 MEAN S.D. N 0.5 MEAN S.D. N 2.0 MEAN S.D. N 5.0 MEAN S.D. N 20.0 MEAN S.D. N 5 1.3 5 5 3.3 5 5 3.0 5 2 1.8 5 5 1.9 5 Covance 6329-183 3M T-6295 240 Table 16 Summary of Clinical Chemistry Data Females Week 14 (PCOAO) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS DOSE PCOAO ppm T-6295 IU/G 0 MEAN S.D. N 0.5 MEAN S.D. N 2.0 MEAN S.D. N 5.0 MEAN S.D. N 20.0 MEAN S.D. N 2 1.6 5 3 2.4 5 1 .7 5 2 2.6 5 5 2.9 5 Covance 6329-183 3M T-6295 241 Table 17 Summary of Clinical Chemistry Data Males Week 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS DOSE GLU ppm T-6295 MG/DL UN MG/DL CREAT MG/DL T PRO G/DL ALB G/DL GLOB G/DL T BILI MG/DL CHOL MG/DL 0 MEAN 102 13 .4 6.9 4.9 2.0 .2 63 S.D. 6.5 1 . 8 .14 .41 .26 .30 .18 13.4 N 10 10 10 10 10 10 10 10 0.5 MEAN 106 14 .6 7.0 5.0 2.0 .2 53 S.D. 1 1 . 2 1 . 6 .14 .34 .20 .26 .15 16.6 N 10 10 10 10 10 10 10 10 2.0 MEAN 91 13 .5 6.7 4.7 2.0 .1 51 S.D. 13.7 2.0 .10 .34 .16 .25 .12 14.5 N 10 10 10 10 10 10 10 10 5.0 MEAN 99 14 .6 7.0 4.9 2 . 1 .2 57 S.D. 8.9 1.4 .12 .46 .29 .20 .15 6.9 N 10 10 10 10 10 10 10 10 20.0 MEAN 95 16 * .6 6.9 4.9 2.0 .1 37 * S.D. 10.3 1 . 6 .16 .34 .24 .18 .13 12.9 N 10 10 10 10 10 10 10 10 Covance 6329-183 3M T-6295 242 Table 17 Summary of Clinical Chemistry Data Males Week 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS DOSE AST/SGOT ALT/SGPT ppm ' 6295 IU/L IU/L GGT IU/L CA MG/DL I PHOS MG/DL NA MMOL/L K MMOL/L CL MMOL/L 0 MEAN S.D. N 0.5 MEAN S.D. N 2.0 MEAN S.D. N 5.0 MEAN S.D. N 20.0 MEAN S.D. N 111 23.1 10 112 18.8 10 127 20.5 10 129 15.4 10 128 60.7 10 36 7.0 10 41 6.3 10 41 4.7 10 44 13.6 10 65 * 52.9 10 1 1.5 10 1 .7 10 1 1.3 10 1 1.6 10 1 1.6 10 10.5 .28 10 10.5 .29 10 10.4 .20 10 10.5 .31 10 10.5 .23 10 7.5 .57 10 7.8 .64 10 7.8 .64 10 7.5 .57 10 7.8 .58 10 146 .7 10 147 2.6 10 147 1.8 10 147 2.8 10 146 1.3 10 6.2 .37 10 6.5 .32 10 6.4 .46 10 6.1 .37 10 6.4 .52 10 105 1 10 105 2 10 105 2 10 104 2 10 104 1 10 Covance 6329-183 3M T-6295 243 Table 17 Summary of Clinical Chemistry Data Females Week 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS DOSE GLU ppm ' -6295 MG/DL UN MG/DL CREAT MG/DL T PRO G/DL ALB G/DL GLOB G/DL T BILI MG/DL CHOL MG/DL 0 MEAN 106 15 .6 7.6 5.6 2 . 1 .1 75 S.D. 12.0 1.7 .12 .34 .25 .22 .19 14 N 10 10 10 10 10 10 10 10 0.5 MEAN 106 15 .6 8.2 * 6.2 * 2.0 .2 88 S.D. 9.1 3.2 .13 .42 .44 .19 .14 27 N 10 10 10 10 10 10 10 10 2.0 MEAN 108 15 .8 * 7.9 5.9 2.0 .1 87 S.D. 5.9 2.3 .12 .30 .24 .22 .08 23 N 10 10 10 10 10 10 10 10 5.0 MEAN 95 * 15 .6 8.0 5.9 2 . 1 .2 70 S.D. 8.3 2.0 .14 .44 .43 .31 .14 13 N 10 10 10 10 10 10 10 10 20.0 MEAN 99 18 * .6 8.0 6.1 * 1.9 .2 66 S.D. 6.7 2.5 .05 .45 .33 .19 .14 14 N 10 10 10 10 10 10 10 10 Covance 6329-183 3M T-6295 244 Table 17 Summary of Clinical Chemistry Data Females Week 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS DOSE AST/SGOT ALT/SGPT ppm ' 6295 IU/L IU/L GGT IU/L CA MG/DL I PHOS MG/DL NA MMOL/L K MMOL/L CL MMOL/L 0 MEAN S.D. N 0.5 MEAN S.D. N 2.0 MEAN S.D. N 5.0 MEAN S.D. N 20.0 MEAN S.D. N 103 16.3 10 107 17.8 10 103 26.9 10 103 16.5 10 90 20.7 10 34 2.4 10 36 9.1 10 37 17.9 10 34 4.7 10 39 18.2 10 1 1.2 10 1 1.7 10 1 1.4 10 2 1.9 10 1 1.5 10 10.7 .45 10 11.1 .49 10 10.9 .43 10 10.7 .51 10 10.8 .30 10 6.3 .95 10 6.4 1.30 10 6.5 .87 10 6.6 1.14 10 6.5 .77 10 148 2.0 10 148 2.1 10 148 3.0 10 148 2.8 10 148 2.6 10 6.0 .43 10 6.4 .76 10 6.2 .71 10 5.8 .72 10 6.2 .57 10 107 .9 10 106 1.6 10 105 1.4 10 107 1.9 10 106 3.0 10 Covance 6329-183 3M T-6295 245 Table 18 Summary of Clinical Chemistry Data Males Week 27 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS DOSE GLU ppm T-6295 MG/DL UN MG/DL CREAT MG/DL T PRO G/DL ALB G/DL GLOB G/DL T BILI MG/DL CHOL MG/DL 0 MEAN 115 12 .7 7.3 4.9 2.4 .2 89 S.D. 8 . 1 2.3 .10 .43 .28 .34 .12 24 7 N 10 10 10 10 10 10 10 10 0.5 MEAN 117 13 .8 7.1 4.7 2.4 .3 84 S.D. 14.7 1.7 .16 .48 .30 .33 .06 31 N 10 10 10 10 10 10 10 10 2.0 MEAN 114 13 .8 7.2 4.8 2.4 .4 74 S.D. 13.2 2.3 .08 .29 .23 .26 .16 15 5 N 10 10 10 10 10 10 10 10 5.0 MEAN 113 14 .7 7.4 4.9 2.5 .3 72 S.D. 11.7 2.5 .16 .45 .28 .24 .12 28 2 N 10 10 10 10 10 10 10 10 20.0 MEAN 111 17 * .8 7.5 5.1 2.4 .2 46 * S.D. 12. 8 2 . 1 .15 .34 .25 .19 .10 11 6 N 10 10 10 10 10 10 10 10 Covance 6329-183 3M T-6295 246 Table 18 Summary of Clinical Chemistry Data Males Week 27 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS DOSE AST/SGOT .ALT/SGPT ppm T--6295 IU/L IU/L GGT IU/L CA MG/DL I PHOS MG/DL NA MMOL/L K MMOL/L CL MMOL/L 0 MEAN S.D. N 0.5 MEAN S.D. N 2.0 MEAN S.D. N 5.0 20.0 MEAN S.D. N MEAN S.D. N 84 1107.8 81 1103.0 92 22.0 10 80 109.7 103 5103.6 376.2 10 366.1 10 47 1100.5 38 106.3 65 4108.7 1 11.1 101.3 1.036 2 11.1 101.8 1.043 1 11.1 10.6 1.028 1 11.3 101.4 1.057 1 11.2 101.9 1.034 6.7 1.036 6.9 1.059 6.8 1.030 7.1 1.062 7.3 1.092 1461.1 10 1471.8 10 147 101.9 148 101.9 147 102.5 6.7 1.023 7.0 1.053 6.7 1.054 6.9 1.046 6.9 1.065 1042.0 10 104 101.4 104 101.5 104 101.4 104 101.4 Covance 6329-183 3M T-6295 247 Table 18 Summary of Clinical Chemistry Data Females Week 27 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS DOSE GLU ppm T-6295 MG/DL UN MG/DL CREAT MG/DL T PRO G/DL ALB G/DL GLOB G/DL T BILI MG/DL CHOL MG/DL 0 MEAN S.D. N 0.5 MEAN S.D. N 2.0 MEAN S.D. N 5.0 MEAN S.D. N 20.0 MEAN S.D. N 104 7.3 10 98 6.3 10 99 8.2 10 98 10.0 10 94 6.8 10 14 1.3 10 15 2.1 10 15 1.9 10 15 3.2 10 20 * 2.8 10 .9 .17 10 .9 .21 10 1.0 .16 10 .9 .14 10 .9 .13 10 8.9 .37 10 8.4 .66 10 8.4 .71 10 8.3 .39 10 8.7 .55 10 6.4 .41 10 6.2 .59 10 6.0 .65 10 5.9 .28 10 6.4 .37 10 2.5 .20 10 2.3 .29 10 2.4 .26 10 2.3 .29 10 2.3 .31 10 .4 115 .14 26.0 10 10 .3 95 .10 29.0 10 10 .3 78 * .13 16.3 10 10 .3 79 * .15 10.9 10 10 .4 80 * .14 24.4 10 10 Covance 6329-183 3M T-6295 248 Table 18 Summary of Clinical Chemistry Data Females Week 27 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS DOSE AST/SGOT ALT/SGPT ppm T--6295 IU/L IU/L GGT IU/L CA MG/DL I PHOS MG/DL NA MMOL/L K MMOL/L CL MMOL/L 0 MEAN S.D. N 0.5 MEAN S.D. N 2.0 MEAN S.D. N 5.0 MEAN S.D. N 20.0 MEAN S.D. N 119 71.9 10 102 18.6 10 143 116.7 10 117 39.6 10 117 62.6 10 64 49.4 10 34 8.4 10 77 130.6 10 47 25.8 10 64 55.5 10 2 2.0 10 2 1.5 10 2 2.0 10 4 1.7 10 2 2.6 10 11.7 .34 10 11.7 .67 10 11.2 * .38 10 11.2 .37 10 11.5 .44 10 6.7 .77 10 6.5 .74 10 6.4 .96 10 6.7 .75 10 7.3 1.20 10 148 2.1 10 147 2.2 10 148 2.4 10 148 1.9 10 148 2.9 10 5.8 .71 10 5.9 .69 10 5.8 .57 10 6.0 .63 10 6.0 .67 10 106 2.3 10 106 1.9 10 107 1.8 10 108 2.0 10 106 2.0 10 Covance 6329-183 3M T-6295 249 Table 19 Summary of Clinical Chemistry Data Males Week 53 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS DOSE GLU ppm T-6295 MG/DL UN MG/DL CREAT MG/DL T PRO G/DL ALB G/DL GLOB G/DL T BILI MG/DL CHOL MG/DL 0 MEAN 129 10 .6 7.0 4.4 2.6 S.D. 13.2 1.4 .17 .46 .35 .33 N 10 10 10 10 10 10 0.5 MEAN S.D. N 125 12.5 10 12 1.2 10 .7 7.4 4.8 2.6 .09 .26 .26 .28 10 10 10 10 2.0 MEAN 121 12 * .7 7.3 4.7 2.6 S.D. 13.8 1.5 .15 .38 .20 .32 N 10 10 10 10 10 10 5.0 MEAN S.D. N 121 8.0 10 14 * 1.3 10 .7 .16 10 7.6 * .46 10 4.8 .28 10 2.7 .32 10 20.0 MEAN 110 * 13 * .7 7.0 4.6 2.4 S.D. 10.2 2.3 .09 .61 .55 .23 N 999999 .0 120 .07 44 6 10 10 .2 129 .16 53 1 10 10 .1 89 .09 17 7 10 10 .1 92 .12 15 2 10 10 .0 50 * .03 17 8 99 Covance 6329-183 3M T-6295 250 Table 19 Summary of Clinical Chemistry Data Males Week 53 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS DOSE AST/SGOT ALT/SGPT ppm ' 6295 IU/L IU/L GGT IU/L CA MG/DL I PHOS MG/DL NA MMOL/L K MMOL/L CL MMOL/L 0 MEAN S.D. N 0.5 MEAN S.D. N 2.0 MEAN S.D. N 5.0 MEAN S.D. N 20.0 MEAN S.D. N 106 27.0 10 118 38.8 10 111 16.6 10 130 21.9 10 129 70.0 9 54 65.8 10 62 * 51.8 10 40 7.5 10 44 8.3 10 83 * 84.4 9 0 .0 10 0 .5 10 0 .8 10 0 1.3 10 1 1.3 9 10.5 .39 10 11.4 * .41 10 11.0 * .43 10 11.1 * .55 10 10.5 .41 9 6.3 .42 10 7.1 * .34 10 7.2 * .59 10 7.7 * .64 10 7.0 * .61 9 147 1.6 10 148 2.5 10 149 2.4 10 149 3.5 10 148 3.5 9 5.9 .44 10 6.5 .55 10 6.6 .56 10 6.8 * .63 10 6.2 .89 9 106 1.8 10 106 1.9 10 107 1.7 10 107 2.4 10 110 * 2.1 9 Covance 6329-183 3M T-6295 251 Table 19 Summary of Clinical Chemistry Data Females Week 53 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS DOSE GLU ppm T-6295 MG/DL UN MG/DL CREAT MG/DL T PRO G/DL ALB G/DL GLOB G/DL T BILI MG/DL CHOL MG/DL 0 MEAN 111 11 .7 7.7 5.5 2. 2 S.D. 1 0 . 1 2.5 .10 1.04 .81 .38 N 10 10 10 10 10 10 0.5 MEAN 102 13 .7 8 . 1 5.8 2.3 S.D. 8.4 2.3 .25 .44 .34 .19 N 10 10 10 10 10 10 2.0 MEAN S.D. N 96 * 13 .8 8.0 5.5 2.5 12.9 2. 2 .15 .45 .44 .46 10 10 10 10 10 10 5.0 MEAN S.D. N 97 * 14 * .8 8.2 5.8 2.4 6.3 2.9 .19 .31 .32 .30 10 10 10 10 10 10 20.0 MEAN S.D. N 98 * 16 * .8 7.9 5.7 2.2 9.8 2.8 .14 .76 .81 .39 10 10 10 10 10 10 .0 116 .07 19 8 10 10 .2 108 .14 26 1 10 10 .1 101 . 1 1 17 2 10 10 .1 110 .13 49 6 10 10 .0 79 .10 25 5 10 10 Covance 6329-183 3M T-6295 252 Table 19 Summary of Clinical Chemistry Data Females Week 53 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS DOSE AST/SGOT ALT/SGPT ppm ' 6295 IU/L IU/L GGT IU/L CA MG/DL I PHOS MG/DL NA MMOL/L K MMOL/L CL MMOL/L 0 MEAN S.D. N 0.5 MEAN S.D. N 2.0 MEAN S.D. N 5.0 MEAN S.D. N 20.0 MEAN S.D. N 119 61.0 10 115 15.3 10 121 21.4 10 108 25.1 10 93 17.5 10 50 30.2 10 43 7.1 10 54 14.5 10 46 19.5 10 46 17.3 10 0 .3 10 0 .5 10 1 1.3 10 1 1.7 10 0 .3 10 10.6 .54 10 11.1 * .48 10 10.8 .24 10 11.0 .29 10 10.6 .47 10 5.8 .90 10 5.8 .83 10 5.6 .58 10 5.5 .74 10 5.9 .65 10 146 2.4 10 147 3.6 10 147 2.1 10 147 1.8 10 147 3.1 10 5.1 .47 10 5.8 1.04 10 5.1 .45 10 5.4 .65 10 5.3 1.00 10 106 2 10 106 3 10 107 2 10 108 3 10 110 2 10 Covance 6329-183 3M T-6295 253 Table 20 Summary of Clinical Chemistry Data Males Week 105 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS DOSE CHOL ppm T-6295 MG/DL TRIG MG/DL 0 MEAN S.D. N 0.5 MEAN S.D. N 2.0 MEAN S.D. N 5.0 MEAN S.D. N 20.0 MEAN S.D. N 20.0 MEAN S.D. N 153 73.5 11 144 60.8 10 129 38.4 17 123 42.7 25 118 52.2 22 145 40.1 10 199 151.3 11 187 82.7 10 199 120.6 17 150 65.3 25 169 121.9 22 137 53.9 10 Covance 6329-183 3M T-6295 254 Table 20 Summary of Clinical Chemistry Data Females Week 105 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS DOSE CHOL ppm T-6295 MG/DL TRIG MG/DL 0 MEAN S.D. N 0.5 MEAN S.D. N 5.0 MEAN S.D. N 20.0 MEAN S.D. N 20.0 MEAN S.D. N 124 39.6 24 124 25.4 15 114 29.8 15 103 57.2 25 109 23.3 17 123 78.6 24 114 100.1 15 136 92.5 15 96 109.7 25 149 76.2 17 Covance 6329-183 3M T-6295 255 Table 21 Summary of Clinical Chemistry Data Females Day 719 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS DOSE CHOL ppm T-6295 MG/DL TRIG MG/DL 2.0 MEAN S.D. N 124 41.1 9 169 70.4 9 Covance 6329-183 3M T-6295 256 Table 22 Summary of Clinical Urinalysis Data Males Week 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS DOSE U VOL ppm T-6295 ML SP GR U PH 0 MEAN S.D. N 0.5 MEAN S.D. N 2.0 MEAN S.D. N 5.0 MEAN S.D. N 20.0 MEAN S.D. N 21.4 11.72 10 22.6 14.76 10 25.3 16.35 10 31.8 14.52 10 22.6 9.78 10 1.010 .0036 10 1.014 .0080 10 1.01 0 .00 73 10 1.00 9 .00 55 10 1.012 .0069 10 7.0 .16 10 7.0 .24 10 7.0 .33 10 7.2 .24 10 7.0 .00 10 Covance 6329-183 3M T-6295 257 Table 22 Summary of Clinical Urinalysis Data Females Week 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS DOSE U VOL ppm T-6295 ML SP GR U PH 0 MEAN S.D. N 0.5 MEAN S.D. N 2.0 MEAN S.D. N 5.0 MEAN S.D. N 20.0 MEAN S.D. N 10.9 13.48 10 15.2 16.13 10 14.2 17.68 10 13.2 7.12 10 17.5 16.39 10 1.019 .0103 10 1.015 .0106 10 1.015 .0087 10 1.014 .0060 10 1.013 .0083 10 6.8 .26 10 6.8 .24 10 6.8 .26 10 6.8 .24 10 6.8 .26 10 Covance 6329-183 3M T-6295 258 Table 23 Summary of Clinical Urinalysis Data Males Week 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS DOSE U VOL ppm T-6295 ML SP GR U PH 0 MEAN S.D. N 0.5 MEAN S.D. N 2.0 MEAN S.D. N 5.0 MEAN S.D. N 20.0 MEAN S.D. N 28.8 16.45 10 23.5 16.23 10 20.8 17.37 10 25.4 14.27 10 18.5 14.64 10 1.014 .0099 10 1.018 .0140 10 1.018 .0099 10 1.014 .0067 10 1.019 .0073 10 7.2 .34 10 7.1 .39 10 7.0 .37 10 7.0 .33 10 7.0 .28 10 Covance 6329-183 3M T-6295 259 Table 23 Summary of Clinical Urinalysis Data Females Week 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS DOSE U VOL ppm T -6295 ML SP GR U PH 0 MEAN S.D. N 0.5 MEAN S.D. N 2.0 MEAN S.D. N 5.0 20.0 MEAN S.D. N MEAN S.D. N 17.5 21.1004 16.8 17.1806 7.8 6.1043 25.7 14.1200 19.5 17.1803 1.020 .011066 1.016 .010090 1.021 .010082 1.009 .010050 1.015 .011019 6.8 1.026 7.0 1.050 6.8 1.035 6.8 1.035 6.9 1.032 Covance 6329-183 3M T-6295 260 Table 24 Summary of Clinical Urinalysis Data Males Week 27 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS DOSE U VOL ppm T-6295 ML SP GR U PH 0 MEAN S.D. N 0.5 MEAN S.D. N 2.0 MEAN S.D. N 5.0 MEAN S.D. N 20.0 MEAN S.D. N 15.2 7.12 10 1.022 .0105 10 16.2 8.42 10 1.017 .0056 10 16.2 6.79 10 1.019 .0053 10 20.4 15.70 10 1.019 .0107 10 17.9 16.42 10 1.029 .0231 10 6.9 .32 10 7.1 .21 10 7.0 .37 10 7.0 .24 10 6.8 .26 10 Covance 6329-183 3M T-6295 261 Table 24 Summary of Clinical Urinalysis Data Females Week 27 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS DOSE U VOL ppm T-6295 ML SP GR U PH 0 MEAN S.D. N 0.5 MEAN S.D. N 2.0 MEAN S.D. N 5.0 MEAN S.D. N 20.0 MEAN S.D. N 17.6 17.05 10 15.9 12.34 10 15.6 12.72 10 21.6 14.18 10 13.7 11.58 10 1.016 .0142 10 1.016 .0089 10 1.016 .0092 10 1.014 .0104 10 1.022 .0170 10 6.6 .21 10 6.6 .34 10 6.8 .24 10 6.9 .32 10 6.6 .37 10 Covance 6329-183 3M T-6295 262 Table 25 Summary of Clinical Urinalysis Data Males Week 53 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS DOSE U VOL ppm T-6295 ML SP GR U PH 0 MEAN S.D. N 0.5 MEAN S.D. N 2.0 MEAN S.D. N 5.0 MEAN S.D. N 20.0 MEAN S.D. N 12.5 7.75 10 18.0 13.20 10 16.2 7.90 10 11.6 7.53 10 11.0 4.55 9 1.025 .0110 10 1.026 .0123 10 1.021 .0063 10 1.032 .0182 10 1.028 .0074 9 6.6 .32 10 6.7 .26 10 7.1 * .42 9 6.9 .39 10 6.9 .49 9 Covance 6329-183 3M T-6295 263 Table 25 Summary of Clinical Urinalysis Data Females Week 53 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS DOSE U VOL ppm T -6295 ML SP GR U PH 0 MEAN S.D. N 0.5 MEAN S.D. N 2.0 MEAN S.D. N 5.0 MEAN S.D. N 20.0 MEAN S.D. N 9.1 2.00 10 7.7 4.07 10 9.0 5.46 10 8.3 12.54 10 10.1 13.35 10 1.022 .0039 10 1.025 .0070 10 1.025 .0114 10 1.030 .0105 10 1.028 .0117 10 6.5 .33 10 6.6 .52 10 6.5 .33 10 6.6 .41 10 6.2 .26 10 Covance 6329-183 3M T-6295 264 Table 26 Summary of Urine Chemistry Data Males Week 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS DOSE U NA ppm T-6295 MMOL/L U K NA EXC MMOL/L MMOL K EXC MMOL 0 MEAN S.D. N 0.5 MEAN S.D. N 2.0 MEAN S.D. N 5.0 MEAN S.D. N 20.0 MEAN S.D. N 12 3.5 10 19 13.9 10 13 9.5 10 14 7.9 10 17 16.4 10 65 20.2 10 86 53.4 10 65 42.5 10 54 32.0 10 68 39.1 10 .22 .092 9 .29 .096 9 .24 .114 7 .40 .186 7 .28 .086 10 1.18 .258 9 1.35 .191 9 1.18 .211 7 1.47 .289 7 1.23 .175 10 Covance 6329-183 3M T-6295 265 Table 26 Summary of Urine Chemistry Data Females Week 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS DOSE U NA ppm T-6295 MMOL/L U K NA EXC MMOL/L MMOL K EXC MMOL 0 MEAN S.D. N 0.5 MEAN S.D. N 2.0 MEAN S.D. N 5.0 MEAN S.D. N 20.0 MEAN S.D. N 22 10.6 10 23 18.0 10 28 16.7 10 22 12.2 10 20 12.3 10 103 59.9 10 86 74.1 10 80 43.9 10 70 34.1 10 67 40.2 10 .13 .049 9 .15 .071 8 .20 .069 8 .24 * .076 10 .18 .048 8 .56 .198 9 .59 .152 8 .57 .198 8 .75 .222 10 .66 .130 8 Covance 6329-183 3M T-6295 266 Table 27 Summary of Urine Chemistry Data Males Week 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS DOSE U NA ppm T-6295 MMOL/L U K NA EXC MMOL/L MMOL K EXC MMOL 0 MEAN S.D. N 0.5 MEAN S.D. N 2.0 MEAN S.D. N 5.0 MEAN S.D. N 20.0 MEAN S.D. N 20 17.1 10 25 16.5 10 23 12.5 10 22 11.7 10 26 13.2 10 60 50.1 10 87 80.7 10 82 56.0 10 65 34.8 10 89 37.8 10 .43 .255 7 .38 .165 8 .33 .180 8 .44 .104 8 .37 .182 10 1.17 .294 7 1.13 .314 8 1.01 .372 8 1.33 .226 8 1.24 .438 10 Covance 6329-183 3M T-6295 267 Table 27 Summary of Urine Chemistry Data Females Week 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS DOSE U NA ppm T-6295 MMOL/L U K NA EXC MMOL/L MMOL K EXC MMOL 0 MEAN S.D. N 0.5 MEAN S.D. N 2.0 MEAN S.D. N 5.0 MEAN S.D. N 20.0 MEAN S.D. N 32 23.2 9 27 17.6 10 43 21.2 10 16 9.1 10 37 40.5 10 77 53.0 9 70 43.6 10 106 44.1 10 42 25.2 10 69 68.9 10 .22 .058 6 .20 .065 8 .26 .146 10 .31 .085 8 .35 .197 8 .54 .172 6 .56 .212 8 .63 .234 10 .88 .281 8 .65 .259 8 Covance 6329-183 3M T-6295 268 Table 28 Summary of Urine Chemistry Data Males Week 27 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS DOSE U NA ppm T-6295 MMOL/L U K NA EXC MMOL/L MMOL K EXC MMOL 0 MEAN S.D. N 0.5 MEAN S.D. N 2.0 MEAN S.D. N 5.0 MEAN S.D. N 20.0 MEAN S.D. N 22 14.5 10 16 10.0 10 24 9.9 9 21 15.5 10 46 42.4 10 116 67.1 10 77 28.1 10 93 25.3 9 89 65.7 10 139 114.5 10 .29 .153 10 .22 .098 10 .35 .192 9 .30 .122 8 .41 .226 8 1.43 .422 10 1.10 .234 10 1.27 .333 9 1.22 .394 8 1.31 .532 8 Covance 6329-183 3M T-6295 269 Table 28 Summary of Urine Chemistry Data Females Week 27 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS DOSE U NA ppm T-6295 MMOL/L U K NA EXC MMOL/L MMOL K EXC MMOL 0 MEAN S.D. N 0.5 MEAN S.D. N 2.0 MEAN S.D. N 5.0 MEAN S.D. N 20.0 MEAN S.D. N 18 18.0 10 14 9.9 10 21 18.1 10 18 17.2 10 32 37.4 10 71 68.2 10 74 50.2 10 70 49.9 10 67 56.7 10 96 85.7 10 .14 .070 8 .16 .055 9 .19 .083 10 .22 .139 9 .21 .119 10 .58 .241 8 .79 .212 9 .66 .158 10 .84 .280 9 .73 .270 10 Covance 6329-183 3M T-6295 270 Table 29 Summary of Urine Chemistry Data Males Week 53 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS DOSE U NA ppm T-6295 MMOL/L U K NA EXC MMOL/L MMOL K EXC MMOL 0 MEAN S.D. N 0.5 MEAN S.D. N 2.0 MEAN S.D. N 5.0 MEAN S.D. N 20.0 MEAN S.D. N 32 15.5 10 23 18.2 10 21 11.4 10 32 21.5 10 46 20.0 9 133 65.6 10 144 103.4 10 96 30.6 10 168 105.3 10 145 36.4 9 .34 .141 10 .27 .158 9 .31 .168 10 .27 .115 10 .49 .215 9 1.30 .390 10 1.65 .354 9 1.40 .401 10 1.41 .228 10 1.51 .473 9 Covance 6329-183 3M T-6295 271 Table 29 Summary of Urine Chemistry Data Females Week 53 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS DOSE U NA ppm T-6295 MMOL/L U K NA EXC MMOL/L MMOL K EXC MMOL 0 MEAN S.D. N 0.5 MEAN S.D. N 2.0 MEAN S.D. N 5.0 MEAN S.D. N 20.0 MEAN S.D. N 40 14.8 10 28 13.6 10 23 * 8.8 10 37 14.5 10 42 22.7 10 135 42.4 10 132 51.3 10 140 69.9 10 172 80.9 10 164 85.4 10 .36 .122 10 .22 .163 10 .18 .081 10 .21 .147 10 .26 .186 9 1.19 .337 10 .87 * .278 10 .99 .307 10 .76 * .239 10 .93 .226 9 Covance 6329-183 3M T-6295 272 TABLE 30 Summary of Organ Weight Data Week 14 Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=2;SUBSET=ALL LF ADRENAL SEX DOSE GROUP M1 NUMBER IN GROUP MEAN: STANDARD DEV TERMINAL BODY WT (g) 5 495.5 55.9 ORGAN WEIGHT (g) 5 0.0331 0.0016 ORGAN-TOBODY WT (%) 5 00..00001608 ORGAN-TOBRAIN WT RATIO 5 00..00011511 SEX DOSE GROUP F1 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT (g) 5 284.0 39.2 ORGAN WEIGHT (g) 5 0.0443 0.0073 M2 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 481.0 51.3 5 0.0296 0.0071 5 00..00001611 5 0.0133 0.0032 F2 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 298.9 40.8 5 0.0346 0.0062 M3 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 434.1 31.4 5 0.0305 0.0082 5 0.0071 0.0023 5 0.0133 0.0032 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 265.7 16.3 5 0.0383 0.0060 1 ^1 1 1 1 1 OJ 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 Covance 6329-183 3M T-6295 PAGE: 1 ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO 5 0.0159 0.0038 5 0.0217 0.0042 5 0.0115 0.0009 5 0.0166 0.0034 5 0.0146 0.0034 5 0.0189 0.0028 273 Table 30 Summary of Organ Weight Data Week 14 Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=2;SUBSET=ALL LF ADRENAL SEX DOSE GROUP M4 TERMINAL BODY WT (g) ORGAN WEIGHT (g) ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO SEX DOSE GROUP F4 TERMINAL BODY WT (g) ORGAN WEIGHT (g) Covance 6329-183 3M T-6295 PAGE: 2 ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO NUMBER IN GROUP MEAN STANDARD DEV 5 524.2 44.5 5 0.0330 0.0063 5 0.0063 0.0008 5 0.0146 0.0034 NUMBER IN GROUP MEAN STANDARD DEV 5 246.7 17.5 5 0.0355 0.0072 5 0.0144 0.0026 5 0.0172 0.0037 11111111111111111111111111111111111111111111111111111111111111 ^ ui in 2 NUMBER IN GROUP MEAN STANDARD DEV 5 470.3 40.1 5 0.0250 0.0032 5 0.0053 0.0004 5 0.0114 0.0005 NUMBER IN GROUP MEAN STANDARD DEV 5 249.1 26.1 5 0.0345 0.0043 5 0.0140 0.0024 5 0.0172 0.0035 11111111111111111111111111111111111111111111111111111111111111 274 Table 30 Summary of Organ Weight Data Week 14 Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=2;SUBSET=ALL RT ADRENAL SEX DOSE GROUP M1 NUMBER IN GROUP MEAN: STANDARD DEV TERMINAL BODY WT (g) 5 495.5 55.9 ORGAN WEIGHT (g) 5 0.0302 0.0051 ORGAN-TOBODY WT (%) 5 0.0062 0.0013 ORGAN-TOBRAIN WT RATIO 5 0.0137 0.0021 SEX DOSE GROUP F1 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT (g) 5 284.0 39.2 ORGAN WEIGHT (g) 5 0.0417 0.0093 M2 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 481.0 51.3 5 0.0247 0.0094 5 0.0051 0.0016 5 0.0112 0.0043 F2 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 298.9 40.8 5 0.0322 0.0056 M3 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 434.1 31.4 5 0.0286 0.0079 5 0.0066 0.0019 5 0.0126 0.0034 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 265.7 16.3 5 0.0373 0.0052 1 ^1 1 1 1 1 OJ 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 Covance 6329-183 3M T-6295 PAGE: 3 ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO 5 0.0150 0.0043 5 0.0204 0.0050 5 0.0108 0.0013 5 0.0154 0.0028 5 0.0141 0.0025 5 0.0184 0.0022 275 Table 30 Summary of Organ Weight Data Week 14 Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=2;SUBSET=ALL RT ADRENAL SEX DOSE GROUP M4 TERMINAL BODY WT (g) ORGAN WEIGHT (g) ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO SEX DOSE GROUP F4 TERMINAL BODY WT (g) ORGAN WEIGHT (g) Covance 6329-183 3M T-6295 PAGE: 4 ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO NUMBER IN GROUP MEAN STANDARD DEV 5 524.2 44.5 5 0.0318 0.0072 5 0.0060 0.0010 5 0.0140 0.0032 NUMBER IN GROUP MEAN STANDARD DEV 5 246.7 17.5 5 0.0329 0.0042 5 0.0133 0.0013 5 0.0159 0.0018 11111111111111111111111111111111111111111111111111111111111111 ^ ui in 2 NUMBER IN GROUP MEAN STANDARD DEV 5 470.3 40.1 5 0.0253 0.0044 5 0.0054 0.0009 5 0.0115 0.0012 NUMBER IN GROUP MEAN STANDARD DEV 5 249.1 26.1 5 0.0323 0.0052 5 0.0131 0.0028 5 0.0161 0.0041 11111111111111111111111111111111111111111111111111111111111111 276 Table 30 Summary of Organ Weight Data Week 14 Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=2;SUBSET=ALL BRAIN SEX DOSE GROUP M1 NUMBER IN GROUP MEAN: STANDARD DEV TERMINAL BODY WT (g) 5 495.5 55.9 ORGAN WEIGHT (g) 5 2.2027 0.0998 ORGAN-TOBODY WT (%) 5 0.4499 0.0620 ORGAN-TOBRAIN WT RATIO 5 1.0000 0.0000 SEX DOSE GROUP F1 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT (g) 5 284.0 39.2 ORGAN WEIGHT (g) 5 2.0506 0.0895 M2 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 481.0 51.3 5 2.2216 0.1022 5 0.4652 0.0408 5 1.0000 0.0000 F2 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 298.9 40.8 5 2.0992 0.1878 M3 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 434.1 31.4 5 2.2768 0.0980 5 0.5280 0.0621 5 1.0000 0.0000 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 265.7 16.3 5 2.0240 0.0532 1 ^1 1 1 1 1 OJ 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 Covance 6329-183 3M T-6295 PAGE: 5 ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO 5 0.7302 0.0796 5 1.0000 0.0000 5 0.7123 0.1139 5 1.0000 0.0000 5 0.7647 0.0623 5 1.0000 0.0000 277 Table 30 Summary of Organ Weight Data Week 14 Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=2;SUBSET=ALL BRAIN SEX DOSE GROUP M4 TERMINAL BODY WT (g) ORGAN WEIGHT (g) ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO SEX DOSE GROUP F4 TERMINAL BODY WT (g) ORGAN WEIGHT (g) Covance 6329-183 3M T-6295 PAGE: 6 ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO NUMBER IN GROUP MEAN STANDARD DEV 5 524.2 44.5 5 2.2740 0.1332 5 0.4366 0.0471 5 1.0000 0.0000 NUMBER IN GROUP MEAN STANDARD DEV 5 246.7 17.5 5 2.0631 0.0817 5 0.8388 0.0509 5 1.0000 0.0000 11111111111111111111111111111111111111111111111111111111111111 ^ ui in 2 NUMBER IN GROUP MEAN STANDARD DEV 5 470.3 40.1 5 2.1876 0.2136 5 0.4655 0.0318 5 1.0000 0.0000 NUMBER IN GROUP MEAN STANDARD DEV 5 249.1 26.1 5 2.0375 0.2129 5 0.8187 0.0414 5 1.0000 0.0000 11111111111111111111111111111111111111111111111111111111111111 278 Table 30 Summary of Organ Weight Data Week 14 Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=2;SUBSET=ALL LF KIDNEY SEX DOSE GROUP M1 NUMBER IN GROUP MEAN: STANDARD DEV TERMINAL BODY WT (g) 5 495.5 55.9 ORGAN WEIGHT (g) 5 1.9158 0.2630 ORGAN-TOBODY WT (%) 5 0.3867 0.0350 ORGAN-TOBRAIN WT RATIO 5 0.8733 0.1428 SEX DOSE GROUP F1 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT (g) 5 284.0 39.2 ORGAN WEIGHT (g) 5 1.1576 0.1245 M2 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 481.0 51.3 5 1.8856 0.2315 5 0.3920 0.0241 5 0.8470 0.0779 F2 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 298.9 40.8 5 1.1245 0.1201 M3 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 434.1 31.4 5 1.8856 0.1583 5 0.4356 0.0413 5 0.8295 0.0808 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 265.7 16.3 5 1.1610 0.0565 1 ^1 1 1 1 1 OJ 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 Covance 6329-183 3M T-6295 PAGE: 7 ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO 5 0.4123 0.0616 5 0.5650 0.0618 5 0.3787 0.0384 5 0.5385 0.0695 5 0.4377 0.0231 5 0.5741 0.0345 279 Table 30 Summary of Organ Weight Data Week 14 Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=2;SUBSET=ALL LF KIDNEY SEX DOSE GROUP M4 TERMINAL BODY WT (g) ORGAN WEIGHT (g) ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO SEX DOSE GROUP F4 TERMINAL BODY WT (g) ORGAN WEIGHT (g) Covance 6329-183 3M T-6295 PAGE: 8 ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO NUMBER IN GROUP MEAN STANDARD DEV 5 524.2 44.5 5 2.0510 0.2638 5 0.3919 0.0448 5 0.9034 0.1208 NUMBER IN GROUP MEAN STANDARD DEV 5 246.7 17.5 5 1.1187 0.1230 5 0.4531 0.0345 5 0.5420 0.0551 11111111111111111111111111111111111111111111111111111111111111 ^ ui in 2 NUMBER IN GROUP MEAN STANDARD DEV 5 470.3 40.1 5 1.9496 0.1517 5 0.4174 0.0525 5 0.9001 0.1341 NUMBER IN GROUP MEAN STANDARD DEV 5 249.1 26.1 5 1.1562 0.0619 5 0.4675 0.0456 5 0.5708 0.0446 11111111111111111111111111111111111111111111111111111111111111 280 Table 30 Summary of Organ Weight Data Week 14 Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=2;SUBSET=ALL RT KIDNEY SEX DOSE GROUP M1 NUMBER IN GROUP MEAN: STANDARD DEV TERMINAL BODY WT (g) 5 495.5 55.9 ORGAN WEIGHT (g) 5 1.8989 0.1660 ORGAN-TOBODY WT (%) 5 0.3849 0.0314 ORGAN-TOBRAIN WT RATIO 5 0.8647 0.0988 SEX DOSE GROUP F1 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT (g) 5 284.0 39.2 ORGAN WEIGHT (g) 5 1.1863 0.1066 M2 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 481.0 51.3 5 1.8373 0.1452 5 0.3834 0.0238 5 0.8262 0.0350 F2 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 298.9 40.8 5 1.1307 0.1396 M3 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 434.1 31.4 5 1.9428 0.1201 5 0.4487 0.0321 5 0.8551 0.0730 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 265.7 16.3 5 1.1545 0.0706 1 ^1 1 1 1 1 OJ 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 Covance 6329-183 3M T-6295 PAGE: 9 ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO 5 0.4224 0.0567 5 0.5792 0.0548 5 0.3796 0.0294 5 0.5417 0.0788 5 0.4347 0.0131 5 0.5713 0.0472 281 Table 30 Summary of Organ Weight Data Week 14 Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=2;SUBSET=ALL RT KIDNEY SEX DOSE GROUP M4 TERMINAL BODY WT (g) ORGAN WEIGHT (g) ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO SEX DOSE GROUP F4 TERMINAL BODY WT (g) ORGAN WEIGHT (g) Covance 6329-183 3M T-6295 PAGE: 10 ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO NUMBER IN GROUP MEAN STANDARD DEV 5 524.2 44.5 5 2.0560 0.2362 5 0.3930 0.0415 5 0.9070 0.1245 NUMBER IN GROUP MEAN STANDARD DEV 5 246.7 17.5 5 1.1446 0.1468 5 0.4626 0.0307 5 0.5543 0.0637 11111111111111111111111111111111111111111111111111111111111111 ^ ui in 2 NUMBER IN GROUP MEAN STANDARD DEV 5 470.3 40.1 5 1.9357 0.1823 5 0.4152 0.0634 5 0.8945 0.1506 NUMBER IN GROUP MEAN STANDARD DEV 5 249.1 26.1 5 1.1993 0.0960 5 0.4844 0.0497 5 0.5914 0.0490 11111111111111111111111111111111111111111111111111111111111111 282 Table 30 Summary of Organ Weight Data Week 14 Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=2;SUBSET=ALL LIVER SEX DOSE GROUP M1 NUMBER IN GROUP MEAN: STANDARD DEV: TERMINAL BODY WT (g) ORGAN WEIGHT (g) 5 495.5 55.9 5 15.8246 1.1470 ORGAN-TOBODY WT (%) 5 3.2105 0.2501 ORGAN-TOBRAIN WT RATIO 5 7.2089 0.7794 SEX DOSE GROUP F1 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT (g) 5 284.0 39.2 ORGAN WEIGHT (g) 5 9.2939 1.5941 M2 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 481.0 51.3 5 15.5356 2.6697 5 3.2119 0.2424 5 6.9828 1.1308 F2 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 298.9 40.8 5 9.1934 1.2506 M3 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 434.1 31.4 5 14.0264 1.3506 5 3.2296 0.1645 5 6.1829 0.7912 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 265.7 16.3 5 8.4060 0.6870 1 ^1 1 1 1 1 OJ 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 Covance 6329-183 3M T-6295 PAGE: 11 ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO 5 3.2663 0.2219 5 4.5286 0.6977 5 3.0785 0.1365 5 4.3942 0.5841 5 3.1686 0.2526 5 4.1509 0.2786 283 Table 30 Summary of Organ Weight Data Week 14 Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=2;SUBSET=ALL LIVER SEX DOSE GROUP M4 TERMINAL BODY WT (g) ORGAN WEIGHT (g) ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO SEX DOSE GROUP F4 TERMINAL BODY WT (g) ORGAN WEIGHT (g) Covance 6329-183 3M T-6295 PAGE: 12 ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO NUMBER IN GROUP MEAN STANDARD DEV 5 524.2 44.5 5 18.8254 3.0304 5 3.5758 0.3075 5 8.3121 1.5632 NUMBER IN GROUP MEAN STANDARD DEV 5 246.7 17.5 5 8.7074 1.0293 5 3.5252 0.2630 5 4.2249 0.5225 11111111111111111111111111111111111111111111111111111111111111 ^ ui in 2 NUMBER IN GROUP MEAN STANDARD DEV 5 470.3 40.1 5 20.3339* 2.2244 5 4.3329* 0.4281 5 9.3597* 1.3157 NUMBER IN GROUP MEAN STANDARD DEV 5 249.1 26.1 5 10.5929 0.6685 5 4.2760* 0.3527 5 5.2213 0.3041 111111111111111111111111111111111111111111111111111111111111111 284 Table 30 Summary of Organ Weight Data Week 14 Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 13 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=2;SUBSET=ALL LUNG SEX DOSE GROUP M1 NUMBER IN GROUP MEAN: STANDARD DEV TERMINAL BODY WT (g) 5 495.5 55.9 ORGAN WEIGHT (g) 5 1.9239 0.1514 ORGAN-TOBODY WT (%) 5 0.3910 0.0406 ORGAN-TOBRAIN WT RATIO 5 0.8773 0.1103 SEX DOSE GROUP F1 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT (g) 5 284.0 39.2 ORGAN WEIGHT (g) 5 1.4837 0.1221 ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO 5 0.5274 0.0581 5 0.7235 0.0500 M2 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 481.0 51.3 5 1.7946 0.1266 5 0.3760 0.0428 5 0.8099 0.0776 F2 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 298.9 40.8 5 1.4581 0.0258 5 0.4954 0.0702 5 0.6992 0.0644 1 ^ 11111 OJ 111111111111111111111111111111111111111111111111111111111 M3 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 434.1 31.4 5 1.8522 0.2526 5 0.4291 0.0704 5 0.8137 0.1089 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 265.7 16.3 5 1.2902* 0.1419 5 0.4859 0.0476 5 0.6369 0.0603 285 Table 30 Summary of Organ Weight Data Week 14 Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=2;SUBSET=ALL LUNG SEX DOSE GROUP M4 TERMINAL BODY WT (g) ORGAN WEIGHT (g) ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO SEX DOSE GROUP F4 TERMINAL BODY WT (g) ORGAN WEIGHT (g) Covance 6329-183 3M T-6295 PAGE: 14 ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO NUMBER IN GROUP MEAN STANDARD DEV 5 524.2 44.5 5 2.6655 1.5011 5 0.5072 0.2769 5 1.1500 0.5633 NUMBER IN GROUP MEAN STANDARD DEV 5 246.7 17.5 5 1.3227* 0.1112 5 0.5390 0.0628 5 0.6420 0.0599 11111111111111111111111111111111111111111111111111111111111111 ^ ui in 2 NUMBER IN GROUP MEAN STANDARD DEV 5 470.3 40.1 5 1.7721 0.1713 5 0.3767 0.0158 5 0.8107 0.0351 NUMBER IN GROUP MEAN STANDARD DEV 5 249.1 26.1 5 1.2912* 0.1316 5 0.5210 0.0596 5 0.6394 0.0970 11111111111111111111111111111111111111111111111111111111111111 286 Table 30 Summary of Organ Weight Data Week 14 Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=2;SUBSET=ALL LF OVARY SEX DOSE GROUP M1 NUMBER IN GROUP MEAN: STANDARD DEV: TERMINAL BODY WT (g) 5 495.5 55.9 ORGAN WEIGHT (g) ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO SEX DOSE GROUP F1 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT (g) 5 284.0 39.2 ORGAN WEIGHT (g) 5 0.0807 0.0147 M2 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 481.0 51.3 F2 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 298.9 40.8 5 0.0707 0.0169 M3 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 434.1 31.4 F3 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 265.7 16.3 5 0.0674 0.0094 Covance 6329-183 3M T-6295 PAGE: 15 ORGAN-TOBODY WT (%) ORGAN-TO BRAIN WT RATIO 5 0.0292 0.0081 5 0.0394 0.0069 5 0.0235 0.0027 5 0.0339 0.0080 5 0.0254 0.0035 5 0.0333 0.0049 287 Table 30 Summary of Organ Weight Data Week 14 Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=2;SUBSET=ALL LF OVARY SEX DOSE GROUP M4 TERMINAL BODY WT (g) ORGAN WEIGHT (g) ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO SEX DOSE GROUP F4 TERMINAL BODY WT (g) ORGAN WEIGHT (g) Covance 6329-183 3M T-6295 PAGE: 16 ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO 11111111111111111111111111111111111111111111111111111111111111 NUMBER IN GROUP: MEAN: STANDARD DEV: M5 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 524.2 44.5 5 470.3 40.1 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 246.7 17.5 5 0.0768 0.0129 5 0.0311 0.0047 5 0.0371 0.0058 ^ ui NUMBER IN GROUP: MEAN: STANDARD DEV: 5 249.1 26.1 5 0.0737 0.0164 5 0.0296 0.0056 5 0.0362 0.0068 288 Table 30 Summary of Organ Weight Data Week 14 Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=2;SUBSET=ALL RT OVARY SEX DOSE GROUP M1 NUMBER IN GROUP MEAN: STANDARD DEV: TERMINAL BODY WT (g) 5 495.5 55.9 ORGAN WEIGHT (g) ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO SEX DOSE GROUP F1 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT (g) 5 284.0 39.2 ORGAN WEIGHT (g) 5 0.0731 0.0060 M2 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 481.0 51.3 F2 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 298.9 40.8 5 0.0700 0.0180 M3 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 434.1 31.4 F3 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 265.7 16.3 5 0.0707 0.0154 Covance 6329-183 3M T-6295 PAGE: 17 ORGAN-TOBODY WT (%) ORGAN-TO BRAIN WT RATIO 5 0.0261 0.0043 5 0.0358 0.0040 5 0.0233 0.0038 5 0.0333 0.0073 5 0.0266 0.0053 5 0.0349 0.0074 289 Table 30 Summary of Organ Weight Data Week 14 Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=2;SUBSET=ALL RT OVARY SEX DOSE GROUP M4 TERMINAL BODY WT (g) ORGAN WEIGHT (g) ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO SEX DOSE GROUP F4 TERMINAL BODY WT (g) ORGAN WEIGHT (g) Covance 6329-183 3M T-6295 PAGE: 18 ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO 11111111111111111111111111111111111111111111111111111111111111 NUMBER IN GROUP: MEAN: STANDARD DEV: M5 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 524.2 44.5 5 470.3 40.1 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 246.7 17.5 5 0.0769 0.0191 5 0.0310 0.0066 5 0.0373 0.0094 ^ ui NUMBER IN GROUP: MEAN: STANDARD DEV: 5 249.1 26.1 5 0.0699 0.0086 5 0.0280 0.0012 5 0.0343 0.0018 290 Table 30 Summary of Organ Weight Data Week 14 Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=2;SUBSET=ALL SPLEEN SEX DOSE GROUP M1 NUMBER IN GROUP MEAN: STANDARD DEV TERMINAL BODY WT (g) 5 495.5 55.9 ORGAN WEIGHT (g) 5 0.7759 0.0794 ORGAN-TOBODY WT (%) 5 0.1575 0.0174 ORGAN-TOBRAIN WT RATIO 5 0.3526 0.0364 SEX DOSE GROUP F1 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT (g) 5 284.0 39.2 ORGAN WEIGHT (g) 5 0.5129 0.0444 M2 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 481.0 51.3 5 0.7825 0.1814 5 0.1612 0.0229 5 0.3510 0.0728 F2 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 298.9 40.8 5 0.5494 0.1033 M3 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 434.1 31.4 5 0.7287 0.0928 5 0.1686 0.0250 5 0.3201 0.0404 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 265.7 16.3 5 0.5110 0.0445 1 ^1 1 1 1 1 OJ 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 Covance 6329-183 3M T-6295 PAGE: 19 ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO 5 0.1822 0.0196 5 0.2501 0.0174 5 0.1845 0.0302 5 0.2617 0.0415 5 0.1926 0.0160 5 0.2529 0.0275 291 Table 30 Summary of Organ Weight Data Week 14 Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=2;SUBSET=ALL SPLEEN SEX DOSE GROUP M4 TERMINAL BODY WT (g) ORGAN WEIGHT (g) ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO SEX DOSE GROUP F4 TERMINAL BODY WT (g) ORGAN WEIGHT (g) Covance 6329-183 3M T-6295 PAGE: 20 ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO NUMBER IN GROUP MEAN STANDARD DEV 5 524.2 44.5 5 0.7046 0.1067 5 0.1341 0.0120 5 0.3120 0.0619 NUMBER IN GROUP MEAN STANDARD DEV 5 246.7 17.5 5 0.4750 0.0247 5 0.1930 0.0099 5 0.2303 0.0107 11111111111111111111111111111111111111111111111111111111111111 ^ ui in 2 NUMBER IN GROUP MEAN STANDARD DEV 5 470.3 40.1 5 0.7754 0.0826 5 0.1650 0.0135 5 0.3543 0.0103 NUMBER IN GROUP MEAN STANDARD DEV 5 249.1 26.1 5 0.4326* 0.0310 5 0.1749 0.0182 5 0.2141 0.0255 11111111111111111111111111111111111111111111111111111111111111 292 Table 30 Summary of Organ Weight Data Week 14 Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=2;SUBSET=ALL LF TESTIS SEX DOSE GROUP M1 NUMBER IN GROUP MEAN: STANDARD DEV TERMINAL BODY WT (g) 5 495.5 55.9 ORGAN WEIGHT (g) 5 1.7498 0.2466 ORGAN-TOBODY WT (%) 5 0.3560 0.0582 ORGAN-TOBRAIN WT RATIO 5 0.7964 0.1237 SEX DOSE GROUP F1 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT (g) 5 284.0 39.2 ORGAN WEIGHT (g) M2 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 481.0 51.3 5 1.7146 0.1818 5 0.3581 0.0344 5 0.7711 0.0617 F2 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 298.9 40.8 M3 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 434.1 31.4 5 1.6809 0.0700 5 0.3887 0.0308 5 0.7394 0.0461 F3 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 265.7 16.3 Covance 6329-183 3M T-6295 PAGE: 21 ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO 293 Table 30 Summary of Organ Weight Data Week 14 Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=2;SUBSET=ALL LF TESTIS SEX DOSE GROUP M4 TERMINAL BODY WT (g) ORGAN WEIGHT (g) ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO SEX DOSE GROUP F4 TERMINAL BODY WT (g) ORGAN WEIGHT (g) Covance 6329-183 3M T-6295 PAGE: 22 ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO in 2 NUMBER IN GROUP: MEAN: STANDARD DEV: NUMBER IN GROUP: MEAN: STANDARD DEV: 5 524.2 44.5 5 1.6548 0.1429 5 470.3 40.1 5 1.6454 0.1290 5 0.3177 0.0390 5 0.3504 0.0163 5 0.7269 0.0220 5 0.7561 0.0726 NUMBER IN GROUP: MEAN: STANDARD DEV: F5 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 246.7 17.5 5 249.1 26.1 11111111111111111111111111111111111111111111111111111111111111 294 Table 30 Summary of Organ Weight Data Week 14 Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=2;SUBSET=ALL RT TESTIS SEX DOSE GROUP M1 NUMBER IN GROUP MEAN: STANDARD DEV TERMINAL BODY WT (g) 5 495.5 55.9 ORGAN WEIGHT (g) 5 1.7446 0.2362 ORGAN-TOBODY WT (%) 5 0.3544 0.0524 ORGAN-TOBRAIN WT RATIO 5 0.7945 0.1246 SEX DOSE GROUP F1 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT (g) 5 284.0 39.2 ORGAN WEIGHT (g) M2 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 481.0 51.3 5 1.6989 0.1610 5 0.3548 0.0310 5 0.7642 0.0531 F2 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 298.9 40.8 M3 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 434.1 31.4 5 1.6716 0.0876 5 0.3863 0.0289 5 0.7356 0.0557 F3 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 265.7 16.3 Covance 6329-183 3M T-6295 PAGE: 23 ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO 295 Table 30 Summary of Organ Weight Data Week 14 Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=2;SUBSET=ALL RT TESTIS SEX DOSE GROUP M4 TERMINAL BODY WT (g) ORGAN WEIGHT (g) ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO SEX DOSE GROUP F4 TERMINAL BODY WT (g) ORGAN WEIGHT (g) Covance 6329-183 3M T-6295 PAGE: 24 ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO in 2 NUMBER IN GROUP: MEAN: STANDARD DEV: NUMBER IN GROUP: MEAN: STANDARD DEV: 5 524.2 44.5 5 1.6615 0.1437 5 470.3 40.1 5 1.6738 0.0932 5 0.3190 0.0402 5 0.3567 0.0134 5 0.7299 0.0263 5 0.7686 0.0513 NUMBER IN GROUP: MEAN: STANDARD DEV: F5 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 246.7 17.5 5 249.1 26.1 11111111111111111111111111111111111111111111111111111111111111 296 Table 30 Summary of Organ Weight Data Week 14 Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=2;SUBSET=ALL LF THYROID/PARA SEX DOSE GROUP M1 NUMBER IN GROUP MEAN: STANDARD DEV TERMINAL BODY WT (g) 5 495.5 55.9 ORGAN WEIGHT (g) 5 0.0162 0.0098 ORGAN-TOBODY WT (%) 5 0.0032 0.0015 ORGAN-TOBRAIN WT RATIO 5 0.0074 0.0046 SEX DOSE GROUP F1 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT (g) 5 284.0 39.2 ORGAN WEIGHT (g) 5 0.0069 0.0015 M2 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 481.0 51.3 5 0.0121 0.0025 5 0.0025 0.0004 5 0.0054 0.0009 F2 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 298.9 40.8 5 0.0098 0.0025 M3 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 434.1 31.4 5 0.0140 0.0091 5 0.0033 0.0025 5 0.0060 0.0036 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 265.7 16.3 5 0.0080 0.0028 1 ^1 1 1 1 1 OJ 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 Covance 6329-183 3M T-6295 PAGE: 25 ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO 5 0.0024 0.0004 5 0.0033 0.0006 5 0.0033 0.0006 5 0.0047 0.0011 5 0.0030 0.0011 5 0.0040 0.0014 297 Table 30 Summary of Organ Weight Data Week 14 Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=2;SUBSET=ALL LF THYROID/PARA SEX DOSE GROUP M4 TERMINAL BODY WT (g) ORGAN WEIGHT (g) ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO SEX DOSE GROUP F4 TERMINAL BODY WT (g) ORGAN WEIGHT (g) Covance 6329-183 3M T-6295 PAGE: 26 ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO NUMBER IN GROUP: MEAN: STANDARD DEV 5 524.2 44.5 5 0.0143 0.0045 5 0.0028 0.0011 5 0.0063 0.0020 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 246.7 17.5 5 0.0091 0.0008 5 0.0037* 0.0005 5 0.0044 0.0004 11111111111111111111111111111111111111111111111111111111111111 ^ ui in 2 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 470.3 40.1 5 0.0114 0.0016 5 0.0025 0.0005 5 0.0053 0.0013 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 249.1 26.1 5 0.0077 0.0012 5 0.0031* 0.0003 5 0.0038 0.0005 11111111111111111111111111111111111111111111111111111111111111 298 Table 30 Summary of Organ Weight Data Week 14 Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 27 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=2;SUBSET=ALL RT THYROID/PARA SEX DOSE GROUP M1 NUMBER IN GROUP MEAN: STANDARD DEV TERMINAL BODY WT (g) 5 495.5 55.9 ORGAN WEIGHT (g) 5 0.0171 0.0080 ORGAN-TOBODY WT (%) 5 00..00001324 ORGAN-TOBRAIN WT RATIO 5 0.0078 0.0038 SEX DOSE GROUP F1 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT (g) 5 284.0 39.2 ORGAN WEIGHT (g) 5 0.0097 0.0033 ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO 5 0.0035 0.0015 5 0.0047 0.0015 M2 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 481.0 51.3 5 0.0132 0.0038 5 0.0027 0.0006 5 0.0059 0.0016 F2 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 298.9 40.8 50.0110 0.0024 5 0.0037 0.0008 5 0.0052 0.0008 1 ^1 1 1 1 1 OJ 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 M3 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 434.1 31.4 5 0.0126 0.0017 5 0.0029 0.0005 5 0.0055 0.0007 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 265.7 16.3 5 0.0116 0.0062 5 0.0044 0.0023 5 0.0057 0.0029 299 Table 30 Summary of Organ Weight Data Week 14 Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=2;SUBSET=ALL RT THYROID/PARA SEX DOSE GROUP M4 TERMINAL BODY WT (g) ORGAN WEIGHT (g) ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO SEX DOSE GROUP F4 TERMINAL BODY WT (g) ORGAN WEIGHT (g) Covance 6329-183 3M T-6295 PAGE: 28 ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO NUMBER IN GROUP: MEAN: STANDARD DEV 5 524.2 44.5 5 0.0159 0.0058 5 0.0031 0.0013 5 0.0069 0.0023 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 246.7 17.5 5 0.0097 0.0013 5 0.0039 0.0005 5 0.0047 0.0005 11111111111111111111111111111111111111111111111111111111111111 ^ ui in 2 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 470.3 40.1 5 0.0104 0.0014 5 0.0022 0.0004 5 0.0048 0.0008 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 249.1 26.1 5 0.0095 0.0011 5 0.0038 0.0003 5 0.0047 0.0004 11111111111111111111111111111111111111111111111111111111111111 300 Table 30 Summary of Organ Weight Data Week 14 Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=2;SUBSET=ALL UTERUS SEX DOSE GROUP M1 NUMBER IN GROUP MEAN: STANDARD DEV TERMINAL BODY WT (g) 5 495.5 55.9 ORGAN WEIGHT (g) ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO SEX DOSE GROUP F1 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT (g) 5 284.0 39.2 ORGAN WEIGHT (g) 5 0.8644 0.3226 M2 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 481.0 51.3 F2 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 298.9 40.8 5 0.5986 0.1521 M3 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 434.1 31.4 F3 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 265.7 16.3 5 0.8368 0.3843 Covance 6329-183 3M T-6295 PAGE: 29 ORGAN-TOBODY WT (%) ORGAN-TO BRAIN WT RATIO 5 0.3157 0.1459 5 0.4244 0.1667 5 0.2010 0.0486 5 0.2906 0.0974 5 0.3125 0.1335 5 0.4173 0.2021 301 Table 30 Summary of Organ Weight Data Week 14 Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=2;SUBSET=ALL UTERUS SEX DOSE GROUP M4 TERMINAL BODY WT (g) ORGAN WEIGHT (g) ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO SEX DOSE GROUP F4 TERMINAL BODY WT (g) ORGAN WEIGHT (g) Covance 6329-183 3M T-6295 PAGE: 30 ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO 11111111111111111111111111111111111111111111111111111111111111 NUMBER IN GROUP: MEAN: STANDARD DEV: M5 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 524.2 44.5 5 470.3 40.1 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 246.7 17.5 5 0.9249 0.3717 5 0.3815 0.1685 5 0.4478 0.1731 ^ ui NUMBER IN GROUP: MEAN: STANDARD DEV: 5 249.1 26.1 5 0.7984 0.4389 5 0.3306 0.1980 5 0.3974 0.2223 302 TABLE 31 Summary of Organ Weight Data Week 53 Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 1 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=3;SUBSET=ALL LF ADRENAL SEX DOSE GROUP M1 NUMBER IN GROUP MEAN STANDARD DEV TERMINAL BODY WT (g) 9 713.4 105.5 ORGAN WEIGHT (g) 9 0.0313 0.0076 ORGAN-TOBODY WT (%) 9 0.0044 0.0010 ORGAN-TOBRAIN WT RATIO 9 0.0135 0.0036 SEX DOSE GROUP F1 NUMBER IN GROUP MEAN STANDARD DEV TERMINAL BODY WT (g) ORGAN WEIGHT (g) ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO 10 414.5 101.9 10 0.0501 0.0290 10 0.0129 0.0091 10 0 .0235 0 .0135 NUMBER IN GROUP MEAN STANDARD DEV 9 668.6 142.5 9 0.0263 0.0046 9 0.0040 0.0008 9 0.0112 0.0018 F5 NUMBER IN GROUP: MEAN: STANDARD DEV: 10 331.9* 39.7 10 0.0311* 0.0053 10 0.0095 0.0022 10 0 .0141* 0 .0024 1 1 1 1 1 1 1 1 1 ID 1 1 1 1 12 1 303 Table 31 Summary of Organ Weight Data Week 53 Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=3;SUBSET=ALL RT ADRENAL SEX DOSE GROUP M1 NUMBER IN GROUP MEAN STANDARD DEV TERMINAL BODY WT (g) 9 713.4 105.5 ORGAN WEIGHT (g) 9 0.0275 0.0060 ORGAN-TOBODY WT (%) 9 0.0039 0.0010 ORGAN-TOBRAIN WT RATIO 9 0.0119 0.0028 SEX DOSE GROUP F1 NUMBER IN GROUP MEAN STANDARD DEV TERMINAL BODY WT (g) ORGAN WEIGHT (g) 10 414.5 101.9 10 0.0368 0.0072 NUMBER IN GROUP MEAN STANDARD DEV 9 668.6 142.5 9 0.0262 0.0031 9 0.0040 0.0007 9 0.0111 0.0011 F5 NUMBER IN GROUP MEAN STANDARD DEV 10 331.9* 39.7 10 0.0318 0.0037 Covance 6329-183 3M T-6295 PAGE: 2 ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO 10 0.0093 0.0027 10 0 .0172 0 .0032 10 0.0097 0.0016 10 0 .0144* 0 .0019 1 1 1 1 1 1 1 1 1 ID 1 1 1 1 12 1 304 Table 31 Summary of Organ Weight Data Week 53 Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=3;SUBSET=ALL BRAIN SEX DOSE GROUP M1 NUMBER IN GROUP MEAN STANDARD DEV TERMINAL BODY WT (g) 9 713.4 105.5 ORGAN WEIGHT (g) 9 2.3308 0.1344 ORGAN-TOBODY WT (%) 9 0.3320 0.0442 ORGAN-TOBRAIN WT RATIO 9 1.0000 0.0000 SEX DOSE GROUP F1 NUMBER IN GROUP MEAN STANDARD DEV TERMINAL BODY WT (g) ORGAN WEIGHT (g) 10 414.5 101.9 10 2.1293 0.0884 NUMBER IN GROUP MEAN STANDARD DEV 9 668.6 142.5 9 2.3485 0.0730 9 0.3644 0.0722 9 1.0000 0.0000 F5 NUMBER IN GROUP MEAN STANDARD DEV 10 331.9* 39.7 10 2.2148 0.1036 Covance 6329-183 3M T-6295 PAGE: 3 ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO 10 0.5376 0.1129 10 1.0000 0.0000 10 0.6752* 0.0817 10 1.0000 0.0000 1 1 1 1 1 1 1 1 1 ID 1 1 1 1 12 1 305 Table 31 Summary of Organ Weight Data Week 53 Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=3;SUBSET=ALL LF KIDNEY SEX DOSE GROUP M1 NUMBER IN GROUP MEAN STANDARD DEV TERMINAL BODY WT (g) 9 713.4 105.5 ORGAN WEIGHT (g) 9 2.3449 0.1955 ORGAN-TOBODY WT (%) 9 0.3327 0.0397 ORGAN-TOBRAIN WT RATIO 9 1.0078 0.0857 SEX DOSE GROUP F1 NUMBER IN GROUP MEAN STANDARD DEV TERMINAL BODY WT (g) ORGAN WEIGHT (g) 10 414.5 101.9 10 1.3621 0.2284 NUMBER IN GROUP MEAN STANDARD DEV 9 668.6 142.5 9 2.4193 0.2614 9 0.3712 0.0597 9 1.0322 0.1260 F5 NUMBER IN GROUP MEAN STANDARD DEV 10 331.9* 39.7 10 1.3638 0.1321 Covance 6329-183 3M T-6295 PAGE: 4 ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO 10 0.3357 0.0458 10 0.6391 0.1000 10 0.4149* 0.0532 10 0.6171 0.0668 1 1 1 1 1 1 1 1 1 ID 1 1 1 1 12 1 306 Table 31 Summary of Organ Weight Data Week 53 Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=3;SUBSET=ALL RT KIDNEY SEX DOSE GROUP M1 NUMBER IN GROUP MEAN STANDARD DEV TERMINAL BODY WT (g) 9 713.4 105.5 ORGAN WEIGHT (g) 9 2.3577 0.2008 ORGAN-TOBODY WT (%) 9 0.3340 0.0348 ORGAN-TOBRAIN WT RATIO 9 1.0129 0.0842 SEX DOSE GROUP F1 NUMBER IN GROUP MEAN STANDARD DEV TERMINAL BODY WT (g) ORGAN WEIGHT (g) 10 414.5 101.9 10 1.4200 0.2361 NUMBER IN GROUP MEAN STANDARD DEV 9 668.6 142.5 9 2.4080 0.2312 9 0.3690 0.0529 9 1.0271 0.1109 F5 NUMBER IN GROUP MEAN STANDARD DEV 10 331.9* 39.7 10 1.3769 0.1375 Covance 6329-183 3M T-6295 PAGE: 5 ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO 10 0.3498 0.0447 10 0.6665 0.1046 10 0.4193* 0.0574 10 0.6224 0.0631 1 1 1 1 1 1 1 1 1 ID 1 1 1 1 12 1 307 Table 31 Summary of Organ Weight Data Week 53 Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=3;SUBSET=ALL LIVER SEX DOSE GROUP M1 NUMBER IN GROUP MEAN STANDARD DEV TERMINAL BODY WT (g) ORGAN WEIGHT (g) 9 713.4 105.5 9 20.0275 3.0980 ORGAN-TOBODY WT (%) 9 2.8113 0.2044 ORGAN-TOBRAIN WT RATIO 9 8.6130 1.4054 SEX DOSE GROUP F1 NUMBER IN GROUP MEAN STANDARD DEV TERMINAL BODY WT (g) ORGAN WEIGHT (g) 10 414.5 101.9 10 11.5769 3.0344 NUMBER IN GROUP MEAN STANDARD DEV: 9 668.6 142.5 9 26.6325* 5.5590 9 4.0039* 0.5449 9 11.3659* 2.4751 F5 NUMBER IN GROUP MEAN STANDARD DEV 10 331.9* 39.7 10 13.8907 1.8568 Covance 6329-183 3M T-6295 PAGE: 6 ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO 10 2.8031 0.3109 10 5.4312 1.3763 10 4.2047* 0.5156 10 6.2883 0.8921 1 1 1 1 1 1 1 1 1 ID 1 1 1 1 12 1 308 Table 31 Summary of Organ Weight Data Week 53 Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=3;SUBSET=ALL LUNG SEX DOSE GROUP M1 NUMBER IN GROUP MEAN STANDARD DEV TERMINAL BODY WT (g) 9 713.4 105.5 ORGAN WEIGHT (g) 9 2.1599 0.2545 ORGAN-TOBODY WT (%) 9 0.3061 0.0413 ORGAN-TOBRAIN WT RATIO 9 0.9293 0.1174 SEX DOSE GROUP F1 NUMBER IN GROUP MEAN STANDARD DEV TERMINAL BODY WT (g) ORGAN WEIGHT (g) 10 414.5 101.9 10 1.6819 0.2502 NUMBER IN GROUP MEAN STANDARD DEV 9 668.6 142.5 9 2.0477 0.1879 9 0.3151 0.0524 9 0.8719 0.0758 F5 NUMBER IN GROUP MEAN STANDARD DEV 10 331.9* 39.7 10 1.5740 0.0922 Covance 6329-183 3M T-6295 PAGE: 7 ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO 10 0.4215 0.0901 10 0.7913 0.1262 10 0.4797 0.0598 10 0.7114 0.0433 1 1 1 1 1 1 1 1 1 ID 1 1 1 1 12 1 309 Table 31 Summary of Organ Weight Data Week 53 Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=3;SUBSET=ALL LF OVARY SEX DOSE GROUP M1 NUMBER IN GROUP MEAN STANDARD DEV TERMINAL BODY WT (g) 9 713.4 105.5 ORGAN WEIGHT (g) ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO SEX DOSE GROUP F1 NUMBER IN GROUP MEAN STANDARD DEV TERMINAL BODY WT (g) ORGAN WEIGHT (g) 10 414.5 101.9 10 0.0562 0.0192 NUMBER IN GROUP MEAN STANDARD DEV: 9 668.6 142.5 F5 NUMBER IN GROUP MEAN STANDARD DEV 10 331.9* 39.7 10 0.0557 0.0123 Covance 6329-183 3M T-6295 PAGE: 8 ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO 10 0.0138 0.0045 10 0 .0265 0 .0092 10 0.0170 0.0040 10 0 .0253 0 .0061 1 1 1 1 1 1 1 1 1 ID 1 1 1 1 12 1 310 Table 31 Summary of Organ Weight Data Week 53 Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=3;SUBSET=ALL RT OVARY SEX DOSE GROUP M1 NUMBER IN GROUP MEAN STANDARD DEV TERMINAL BODY WT (g) 9 713.4 105.5 ORGAN WEIGHT (g) ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO SEX DOSE GROUP F1 NUMBER IN GROUP MEAN STANDARD DEV: TERMINAL BODY WT (g) ORGAN WEIGHT (g) 10 414.5 101.9 10 0.1357 0.2404 NUMBER IN GROUP MEAN STANDARD DEV 9 668.6 142.5 F5 NUMBER IN GROUP: MEAN: STANDARD DEV: 10 331.9* 39.7 10 0.0561 0.0129 Covance 6329-183 3M T-6295 PAGE: 9 ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO 10 0.0321 0.0549 10 0.0630 0.1107 10 0.0171 0.0044 10 0.0254 0.0062 1 1 1 1 1 1 1 1 1 ID 1 1 1 1 12 1 311 Table 31 Summary of Organ Weight Data Week 53 Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 10 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=3;SUBSET=ALL SPLEEN SEX DOSE GROUP M1 NUMBER IN GROUP MEAN STANDARD DEV TERMINAL BODY WT (g) 9 713.4 105.5 ORGAN WEIGHT (g) 9 0.9792 0.0975 ORGAN-TOBODY WT (%) 9 00..01130802 ORGAN-TOBRAIN WT RATIO 9 0.4208 0.0427 SEX DOSE GROUP F1 NUMBER IN GROUP MEAN STANDARD DEV TERMINAL BODY WT (g) ORGAN WEIGHT (g) ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO 10 414.5 101.9 10 00..15052717 10 0.1368 0.0178 10 0.2611 0.0419 NUMBER IN GROUP MEAN STANDARD DEV 6698.6 142.5 9 0.8287* 0.1514 9 0.1252* 0.0133 9 0.3529* 0.0635 F5 NUMBER IN GROUP MEAN STANDARD DEV 10 331.9* 39.7 10 0.5491 0.1344 10 0.1650* 0.0306 10 0.2468 0.0537 1 1 1 1 1 1 1 1 1 ID 1 1 1 1 12 1 312 Table 31 Summary of Organ Weight Data Week 53 Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=3;SUBSET=ALL LF TESTIS SEX DOSE GROUP M1 NUMBER IN GROUP MEAN STANDARD DEV TERMINAL BODY WT (g) 9 713.4 105.5 ORGAN WEIGHT (g) 9 1.8551 0.3741 ORGAN-TOBODY WT (%) 9 0.2641 0.0658 ORGAN-TOBRAIN WT RATIO 9 0.7968 0.1645 SEX DOSE GROUP F1 NUMBER IN GROUP MEAN STANDARD DEV TERMINAL BODY WT (g) ORGAN WEIGHT (g) 10 414.5 101.9 NUMBER IN GROUP MEAN STANDARD DEV 9 668.6 142.5 9 1.6414 0.3094 9 0.2531 0.0628 9 0.6995 0.1325 F5 NUMBER IN GROUP MEAN STANDARD DEV 10 331.9* 39.7 Covance 6329-183 3M T-6295 PAGE: 11 ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO 11111111 11111 ID 11 2 313 Table 31 Summary of Organ Weight Data Week 53 Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=3;SUBSET=ALL RT TESTIS SEX DOSE GROUP M1 NUMBER IN GROUP MEAN STANDARD DEV TERMINAL BODY WT (g) 9 713.4 105.5 ORGAN WEIGHT (g) 9 1.8759 0.1801 ORGAN-TOBODY WT (%) 9 0.2683 0.0491 ORGAN-TOBRAIN WT RATIO 9 0.8100 0.1189 SEX DOSE GROUP F1 NUMBER IN GROUP MEAN STANDARD DEV TERMINAL BODY WT (g) ORGAN WEIGHT (g) 10 414.5 101.9 NUMBER IN GROUP MEAN STANDARD DEV 9 668.6 142.5 9 1.6551 0.2786 9 0.2560 0.0631 9 0.7054 0.1203 F5 NUMBER IN GROUP MEAN STANDARD DEV 10 331.9* 39.7 Covance 6329-183 3M T-6295 PAGE: 12 ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO 1 1 1 1 1 1 1 1 1 ID 1 1 1 1 12 1 314 Table 31 Summary of Organ Weight Data Week 53 Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=3;SUBSET=ALL LF THYROID/PARA SEX DOSE GROUP M1 NUMBER IN GROUP MEAN STANDARD DEV TERMINAL BODY WT (g) 9 713.4 105.5 ORGAN WEIGHT (g) 9 0.0246 0.0060 ORGAN-TOBODY WT (%) 9 0.0035 0.0008 ORGAN-TOBRAIN WT RATIO 9 0.0106 0.0027 SEX DOSE GROUP F1 NUMBER IN GROUP MEAN STANDARD DEV TERMINAL BODY WT (g) ORGAN WEIGHT (g) 10 414.5 101.9 10 0.0136 0.0062 NUMBER IN GROUP MEAN STANDARD DEV 9 668.6 142.5 9 0.0195* 0.0041 9 0.0029 0.0004 9 0.0083* 0.0018 F5 NUMBER IN GROUP MEAN STANDARD DEV 10 331.9* 39.7 10 0.0115 0.0036 Covance 6329-183 3M T-6295 PAGE: 13 ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO 10 0.0034 0.0018 10 0 .0065 0 .0034 10 0.0034 0.0010 10 0 .0052 0 .0016 1 1 1 1 1 1 1 1 1 ID 1 1 1 1 12 1 315 Table 31 Summary of Organ Weight Data Week 53 Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=3;SUBSET=ALL RT THYROID/PARA SEX DOSE GROUP M1 NUMBER IN GROUP MEAN STANDARD DEV TERMINAL BODY WT (g) 9 713.4 105.5 ORGAN WEIGHT (g) 9 0.0249 0.0048 ORGAN-TOBODY WT (%) 9 0.0035 0.0007 ORGAN-TOBRAIN WT RATIO 9 0.0107 0.0018 SEX DOSE GROUP F1 NUMBER IN GROUP MEAN STANDARD DEV TERMINAL BODY WT (g) ORGAN WEIGHT (g) 10 414.5 101.9 10 0.0148 0.0039 NUMBER IN GROUP MEAN STANDARD DEV 9 668.6 142.5 9 0.0240 0.0093 9 0.0036 0.0013 9 0.0102 0.0040 F5 NUMBER IN GROUP MEAN STANDARD DEV 10 331.9* 39.7 10 0.0124 0.0046 Covance 6329-183 3M T-6295 PAGE: 14 ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO 10 0.0037 0.0011 10 0 .0070 0 .0019 10 0.0038 0.0014 10 0 .0056 0 .0021 1 1 1 1 1 1 1 1 1 ID 1 1 1 1 12 1 316 Table 31 Summary of Organ Weight Data Week 53 Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=3;SUBSET=ALL UTERUS SEX DOSE GROUP M1 NUMBER IN GROUP MEAN STANDARD DEV TERMINAL BODY WT (g) 9 713.4 105.5 ORGAN WEIGHT (g) ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO SEX DOSE GROUP F1 NUMBER IN GROUP MEAN STANDARD DEV TERMINAL BODY WT (g) ORGAN WEIGHT (g) 10 414.5 101.9 10 1.0175 0.3207 NUMBER IN GROUP MEAN STANDARD DEV 9 668.6 142.5 F5 NUMBER IN GROUP MEAN STANDARD DEV 10 331.9* 39.7 10 1.0062 0.3090 Covance 6329-183 3M T-6295 PAGE: 15 ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO 10 0.2557 0.0951 10 0.4771 0.1461 10 0.3111 0.1154 10 0.4587 0.1545 11111111 11111 ID 11 2 317 TABLE 32 Incidence of Macroscopic Observations Week 14 Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 1 TABLE INCLUDES: SEX=ALL;GROUP=1,2,3/4 /5;WEEKS=ALL DEATH=2;SUBSET=ALL -- N U M BE R -O F -A N IMA L S -A F FE SEX: MALE-- FEMALE GROUP: -1 - -2 - -3- -4- -5- -1 - -2 - -3- -4- -5- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 5 5 5 5 5 5 5 5 5 5 ** TOP OF LIST ** GENERAL COMMENT (GC) ............... .................. NUMBER EXAMINED: 5 5 5 5 5 5 5 5 5 5 NO MACROSCOPIC LESIONS BONE MARROW SMEAR TAKEN MALOCCLUSION 31 43325423 4555555555 00001 00000 KIDNEY (KD) ........................ .................. NUMBER EXAMINED: 5 5 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 4 4 4 4 4 5 5 4 5 CYST(S) LARGE PELVIS(ES) GRANULAR MATERIAL 000001 0000 01 1 1 1 0001 0 000000001 0 UTERUS (UT) ........................ .................. NUMBER EXAMINED: 0 0 0 0 0 5 5 5 5 5 NOT REMARKABLE: 0 0 0 0 0 3 5 4 3 3 LUMEN FILLED WITH FLUID 000002 01 2 2 THYMUS (TH) ........................ .................. NUMBER EXAMINED: 5 5 5 5 5 5 5 5 5 5 NOT REMARKABLE: 4 5 5 5 4 5 5 5 5 5 RED FOCUS(I)/AREA(S) 1 0001 00000 318 TABLE 32 Incidence of Macroscopic Observations Week 14 Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 2 TABLE INCLUDES: SEX=ALL;GROUP=1,2,3/4 /5;WEEKS=ALL DEATH=2;SUBSET=ALL -- N U M BE R -O F -A N IMA L S -A F FE SEX: MALE-- FEMALE-- GROUP: -1 - -2 - -3- -4- -5- -1 - -2 - -3- -4 - -5- ORGAN AND KEYWORD(S) OR PHRASE -- -- NUMBER: 5 5 5 5 5 5 5 5 5 5 -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- HARDERIAN GLAND (HG) ............... .................. NUMBER EXAMINED: 5 5 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 4 5 5 5 5 5 5 5 5 MISSING/NOT IDENTIFIED 01 00000000 STOMACH, GL (ST) ................... .................. NUMBER EXAMINED: 5 5 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 4 5 5 5 4 5 5 5 4 DARK FOCUS(I)/AREA(S) 01 0001 0001 LN, MANDIBULAR (MN) ................ .................. NUMBER EXAMINED: 5 5 5 5 5 5 5 5 5 5 NOT REMARKABLE: 4 2 5 4 5 5 5 5 5 5 DIFFUSELY RED ** END OF LIST ** 1 3 01 000000 319 TABLE 33 Incidence of Macroscopic Observations Week 53 Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 1 TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=3;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---SEX: --MALE -- -FEMALEGROUP: -1 - -5- -1 - -5- ORGAN AND KEYWORD(S) OR PHRASE ---- NUMBER: 10 9 10 10 -=- -=- -=- -=- ** TOP OF LIST ** GENERAL COMMENT (GC) ........ ........................ NUMBER EXAMINED: 10 9 10 10 NO MACROSCOPIC LESIONS BONE MARROW SMEAR TAKEN MALOCCLUSION ANIMAL OBESE 531 2 10 9 10 10 1 1 20 21 00 EYE (EY) ..................... ........................ NUMBER EXAMINED: 10 NOT REMARKABLE: 10 9 10 10 9 10 10 BRAIN (BR) ................... ........................ NUMBER EXAMINED: NOT REMARKABLE: COMPRESSED (HYPOTHALAMUS) LIVER (LI) ................... ........................ NUMBER EXAMINED: NOT REMARKABLE: DIFFUSELY DARK KIDNEY (KD) .................. ........................ NUMBER EXAMINED: NOT REMARKABLE: 10 10 0 10 10 0 10 10 9 10 10 9 9 10 01 0 9 10 10 9 10 9 001 9 10 10 9 10 10 320 TABLE 33 Incidence of Macroscopic Observations Week 53 Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 2 TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=3;SUBSET=ALL ORGAN AND KEYWORD(S) OR PHRASE -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---- SEX: --MALE -- -FEMALE- GROUP: -1 - -5- -1 - -5- NUMBER: 10 9 10 10 SPLEEN (SP) .................. ........................ NUMBER EXAMINED: NOT REMARKABLE: CYST(S) PANCREAS (PA) ................ ........................ NUMBER EXAMINED: NOT REMARKABLE: MASS(ES) ADRENAL, CORTEX (AC) ........ ........................ NUMBER EXAMINED: NOT REMARKABLE: LARGE CYST(S) ADRENAL, MEDULLA (MA) ....... ........................ NUMBER EXAMINED: NOT REMARKABLE: 10 10 0 10 10 0 10 10 0 0 10 10 9 10 10 9 10 9 001 9 10 10 9 9 10 01 0 9 10 10 9 8 10 01 01 0 0 9 10 10 9 10 10 LN, MESENTERIC (MS) ......... ........................ NUMBER EXAMINED: 10 NOT REMARKABLE: 10 9 10 10 9 10 10 321 TABLE 33 Incidence of Macroscopic Observations Week 53 Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 3 TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=3;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---SEX: --MALE -- -FEMALEGROUP: -1 - -5- -1 - -5- ORGAN AND KEYWORD(S) OR PHRASE ---- NUMBER: 10 9 10 10 -=- -=- -=- -=- UTERUS (UT) .................. ........................ NUMBER EXAMINED: NOT REMARKABLE: 0 0 0 10 10 099 LUMEN FILLED WITH FLUID THICKENED WALL 0001 001 0 TESTIS (TE) .................. ........................ NUMBER EXAMINED: 10 9 0 0 NOT REMARKABLE: 9 8 0 0 SMALL 1 1 00 BONE, FEMUR (FE) ............. ........................ NUMBER EXAMINED: 10 NOT REMARKABLE: 10 9 10 10 9 10 10 MARROW, FEMUR (FM) .......... ........................ NUMBER EXAMINED: 10 NOT REMARKABLE: 10 9 10 10 9 10 10 MARROW, STERNUM (SE) ........ ........................ NUMBER EXAMINED: 10 NOT REMARKABLE: 10 9 10 10 9 10 10 BONE, STERNUM (SB) .......... ........................ NUMBER EXAMINED: 10 NOT REMARKABLE: 10 9 10 10 9 10 10 322 TABLE 33 Incidence of Macroscopic Observations Week 53 Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 4 TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=3;SUBSET=ALL ORGAN AND KEYWORD(S) OR PHRASE -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---- SEX: --MALE -- -FEMALE- GROUP: -1 - -5- -1 - -5- NUMBER: 10 9 10 10 SPINAL CORD (SC) ............. ........................ NUMBER EXAMINED: 10 NOT REMARKABLE: 10 9 10 10 9 10 10 THYMUS (TH) .................. ........................ NUMBER EXAMINED: NOT REMARKABLE: LARGE GELATINOUS LUNG (LU) .................... ........................ NUMBER EXAMINED: NOT REMARKABLE: LIGHT FOCUS(I)/AREA(S) DARK FOCUS(I)/AREA(S) HEART (HT) ................... ........................ NUMBER EXAMINED: NOT REMARKABLE: 10 10 0 0 10 9 0 1 10 10 9 10 10 8 10 10 1 00 1 00 9 10 10 9 10 8 002 000 9 10 10 9 10 10 MUSCLE, SKELETAL (SM) ....... ........................ NUMBER EXAMINED: 10 NOT REMARKABLE: 10 9 10 10 9 10 10 NERVE, SCIATIC (SN) ......... ........................ NUMBER EXAMINED: 10 NOT REMARKABLE: 10 9 10 10 9 10 10 323 TABLE 33 Incidence of Macroscopic Observations Week 53 Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 5 TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=3;SUBSET=ALL ORGAN AND KEYWORD(S) OR PHRASE -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---- SEX: --MALE -- -FEMALE- GROUP: -1 - -5- -1 - -5- NUMBER: 10 9 10 10 TRACHEA (TR) ................. ........................ NUMBER EXAMINED: 10 NOT REMARKABLE: 10 9 10 10 9 10 10 ESOPHAGUS (ES) ............... ........................ NUMBER EXAMINED: 10 NOT REMARKABLE: 10 9 10 10 9 10 10 THYROID (TY) ................. ........................ NUMBER EXAMINED: 10 NOT REMARKABLE: 10 9 10 10 9 10 10 PARATHYROID (PT) ............. ........................ NUMBER EXAMINED: 10 NOT REMARKABLE: 10 9 10 10 9 10 10 PITUITARY (PI) ............... ........................ NUMBER EXAMINED: NOT REMARKABLE: LARGE DARK FOCUS(I)/AREA(S) RED FOCUS(I)/AREA(S) RAISED AREA(S) CYST(S) HARDERIAN GLAND (HG) ........ ........................ NUMBER EXAMINED: NOT REMARKABLE: 10 9 0 0 1 0 0 10 10 9 10 10 864 02 3 02 0 002 001 1 00 9 10 10 9 10 10 324 TABLE 33 Incidence of Macroscopic Observations Week 53 Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 6 TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=3;SUBSET=ALL ORGAN AND KEYWORD(S) OR PHRASE -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---SEX: --MALE -- -FEMALEGROUP: -1- -5- -1- -5- NUMBER: 10 9 10 10 10SALIV GL, MANDIB (SG) ....... ........................ NUMBER EXAMINED: 10NOT REMARKABLE: 9 9 1100 1100 STOMACH, GL (ST) ............. ........................ NUMBER EXAMINED: NOT REMARKABLE: RED FOCUS(I)/AREA(S) THICKENED WALL STOMACH, NONGL (SU) ......... ........................ NUMBER EXAMINED: NOT REMARKABLE: THICKENED WALL DUODENUM (DU) ................ ........................ NUMBER EXAMINED: NOT REMARKABLE: 1100 00 1100 0 1100 9 7 1100 1100 11 00 00 89 1100 1100 100 9 9 1100 1100 10JEJUNUM (JE) ................. ........................ NUMBER EXAMINED: NOT REMARKABLE: 10 9 10 10 9 10 10 10ILEUM (IL) ................... ........................ NUMBER EXAMINED: 10NOT REMARKABLE: 9 9 1100 1100 325 TABLE 33 Incidence of Macroscopic Observations Week 53 Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 7 TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=3;SUBSET=ALL ORGAN AND KEYWORD(S) OR PHRASE -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---- SEX: --MALE -- -FEMALE- GROUP: -1 - -5- -1 - -5- NUMBER: 10 9 10 10 CECUM (CE) ................... ........................ NUMBER EXAMINED: 10 NOT REMARKABLE: 10 9 10 10 9 10 10 COLON (CO) ................... ........................ NUMBER EXAMINED: 10 NOT REMARKABLE: 10 9 10 10 9 10 10 RECTUM (RE) .................. ........................ NUMBER EXAMINED: 10 NOT REMARKABLE: 10 9 10 10 9 10 10 SKIN (SK) .................... ........................ NUMBER EXAMINED: NOT REMARKABLE: CRUSTED AREA(S) LIGHT AREA(S)/FOCUS(I) MAMMARY, FEMALE (MF) ........ ........................ NUMBER EXAMINED: NOT REMARKABLE: MASS(ES) THICKENED CYST(S) URINARY BLADDER (UB) ........ ........................ NUMBER EXAMINED: NOT REMARKABLE: 10 10 0 0 0 0 0 0 0 10 10 9 10 10 9 8 10 02 0 01 0 0 10 10 0 6 10 01 0 01 0 02 0 9 10 10 9 10 10 326 TABLE 33 Incidence of Macroscopic Observations Week 53 Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 8 TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=3;SUBSET=ALL ORGAN AND KEYWORD(S) OR PHRASE -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---- SEX: --MALE -- -FEMALE- GROUP: -1 - -5- -1 - -5- NUMBER: 10 9 10 10 CERVIX (CV) .................. ........................ NUMBER EXAMINED: NOT REMARKABLE: MASS(ES) OVARY (OV) ................... ........................ NUMBER EXAMINED: NOT REMARKABLE: CYST(S) VAGINA (VA) .................. ........................ NUMBER EXAMINED: NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 10 10 0 9 10 01 0 0 10 10 0 9 10 01 0 0 10 10 0 10 10 PROSTATE (PR) ................ ........................ NUMBER EXAMINED: 10 9 0 0 NOT REMARKABLE: 10 9 0 0 SEMINAL VESICLES (SV) ....... ........................ NUMBER EXAMINED: 10 9 0 0 NOT REMARKABLE: 10 9 0 0 EPIDIDYMIDES (EP) ............ ........................ NUMBER EXAMINED: 10 9 0 0 NOT REMARKABLE: 10 9 0 0 327 TABLE 33 Incidence of Macroscopic Observations Week 53 Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 9 TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=3;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---SEX: --MALE -- -FEMALEGROUP: -1 - -5- -1 - -5- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 10 9 10 10 -------------------------------- ------------------------------------------- -=- -=- -=- -=- CAVITY, THORACIC (TA) ....... ........................ NUMBER EXAMINED: 10 NOT REMARKABLE: 10 9 10 10 9 10 10 TAIL (TI) .................... ........................ NUMBER EXAMINED: 10 NOT REMARKABLE: 10 9 10 10 9 10 10 LN, MANDIBULAR (MN) ......... ........................ NUMBER EXAMINED: NOT REMARKABLE: DIFFUSELY RED MOTTLED LN, MEDIASTINAL (ML) ........ ........................ NUMBER EXAMINED: NOT REMARKABLE: 10 9 0 1 10 10 9 10 10 989 02 1 000 9 10 10 9 10 10 LN, AXILLARY (AX) ............ ........................ NUMBER EXAMINED: 10 NOT REMARKABLE: 10 9 10 10 9 10 10 LN, INGUINAL (IN) ............ ........................ NUMBER EXAMINED: 10 NOT REMARKABLE: 10 9 10 10 9 10 10 328 TABLE 33 Incidence of Macroscopic Observations Week 53 Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 10 TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=3;SUBSET=ALL ORGAN AND KEYWORD(S) OR PHRASE -- N U M B E R - O F - A N I M A L S - A F F E C SEX: --MALE -- -FEMALE- GROUP: -1 - -5- -1 - -5- NUMBER: 10 9 10 10 T E D -- LN, OTHER (LN) ............... ........................ NUMBER EXAMINED: 10 NOT REMARKABLE: 10 9 10 10 9 10 10 LN, RENAL (RL) ............... ........................ NUMBER EXAMINED: 10 NOT REMARKABLE: 10 9 10 10 9 10 10 MAMMARY, MALE (MM) .......... ........................ NUMBER EXAMINED: 10 9 0 0 NOT REMARKABLE: 10 9 0 0 COAGULATING GL (CG) ......... ........................ NUMBER EXAMINED: 10 9 0 0 NOT REMARKABLE: 10 9 0 0 LN, ANT MES/PANC (AP) ....... ........................ NUMBER EXAMINED: 10 NOT REMARKABLE: 10 9 10 10 9 10 10 PREPUTIAL GLAND (PG) ........ ........................ NUMBER EXAMINED: 10 9 0 0 NOT REMARKABLE: 10 9 0 0 CAVITY, ABDOM (PC) .......... ........................ NUMBER EXAMINED: 10 NOT REMARKABLE: 10 9 10 10 9 10 10 329 TABLE 33 Incidence of Macroscopic Observations Week 53 Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 11 TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=3;SUBSET=ALL ORGAN AND KEYWORD(S) OR PHRASE -- N U M B E R - O F - A N I M A L S - A F F E C SEX: --MALE -- -FEMALE- GROUP: -1 - -5- -1 - -5- NUMBER: 10 9 10 10 T E D -- AORTA (AO) ................... ........................ NUMBER EXAMINED: 10 NOT REMARKABLE: 10 9 10 10 9 10 10 FOOT/FOOTPAD (FP) ............ ........................ NUMBER EXAMINED: 10 NOT REMARKABLE: 10 9 10 10 9 10 10 AUDITORY SEB GL (AS) ........ ........................ NUMBER EXAMINED: 10 NOT REMARKABLE: 10 9 10 10 9 10 10 CALVARIUM/SKULL (CA) ........ ........................ NUMBER EXAMINED: 10 NOT REMARKABLE: 10 9 10 10 9 10 10 LN, LUMBAR/ILIAC (LM) ....... ........................ NUMBER EXAMINED: 10 NOT REMARKABLE: 10 9 10 10 9 10 10 URETER (UE) .................. ........................ NUMBER EXAMINED: 10 NOT REMARKABLE: 10 9 10 10 9 10 10 JOINT, OTHER (JT) ............ ........................ NUMBER EXAMINED: 10 NOT REMARKABLE: 10 9 10 10 9 10 10 330 TABLE 33 Incidence of Macroscopic Observations Week 53 Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 12 TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=3;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C SEX: --MALE -- -FEMALEGROUP: -1 - -5- -1 - -5- ORGAN AND KEYWORD(S) OR PHRASE ---- NUMBER: 10 9 10 10 -=- -=- -=- -=- ADIPOSE TISSUE (AT) ......... ........................ NUMBER EXAMINED: 10 NOT REMARKABLE: 10 9 10 10 9 10 10 T E D -- VAS DEFERENS (VS) ............ ........................ NUMBER EXAMINED: 10 9 0 0 NOT REMARKABLE: 10 9 0 0 DIAPHRAGM (DP) ............... ........................ NUMBER EXAMINED: 10 NOT REMARKABLE: 10 9 10 10 9 10 10 LN, POPLITEAL (PP) .......... ........................ NUMBER EXAMINED: 10 NOT REMARKABLE: 10 9 10 10 9 10 10 PERICARDIAL SAC (PD) ........ ........................ NUMBER EXAMINED: 10 NOT REMARKABLE: 10 9 10 10 9 10 10 ARTERY, OTHER (AU) .......... ........................ NUMBER EXAMINED: 10 NOT REMARKABLE: 10 9 10 10 9 10 10 AORTIC ARCH (AA) ............. ........................ NUMBER EXAMINED: 10 NOT REMARKABLE: 10 9 10 10 9 10 10 331 TABLE 33 Incidence of Macroscopic Observations Week 53 Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 13 TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=3;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C SEX: --MALE -- -FEMALEGROUP: -1 - -5- -1 - -5- ORGAN AND KEYWORD(S) OR PHRASE -------------------------------- -- NUMBER: 10 9 10 10 -=- -=- -=- -=- LN, ILEOCECAL (IC) .......... ........................ NUMBER EXAMINED: 10 NOT REMARKABLE: 10 9 10 10 9 10 10 T E D -- LN, TRACHEOBRON (TB) ........ ........................ NUMBER EXAMINED: 10 NOT REMARKABLE: 10 9 10 10 9 10 10 MUSCLE, OTHER (MO) .......... ........................ NUMBER EXAMINED: 10 NOT REMARKABLE: 10 9 10 10 9 10 10 PINNA (PN) ................... ........................ NUMBER EXAMINED: 10 NOT REMARKABLE: 10 9 10 10 9 10 10 BILE DUCT (BD) ............... ........................ NUMBER EXAMINED: 10 NOT REMARKABLE: 10 9 10 10 9 10 10 TRIGEMINAL NERVE (TG) ....... ........................ NUMBER EXAMINED: 10 NOT REMARKABLE: 10 9 10 10 9 10 10 CLITORAL GLAND (CL) ......... ........................ NUMBER EXAMINED: NOT REMARKABLE: ** END OF LIST ** 0 0 0 10 10 0 10 10 332 TABLE 34 Incidence of Macroscopic Observations All Animals 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 1 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C T E D SEX: ------------ MALE------------ ----------- FEMALE GROUP: -1 - -2 - -3- -4- -5- -6- -1 - -2 - -3- -4- -5- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 70 60 60 60 70 40 70 60 60 60 70 ** TOP OF LIST ** GENERAL COMMENT (GC) .................................. NUMBER EXAMINED: 70 60 60 60 70 40 70 60 60 60 70 NO MACROSCOPIC LESIONS BLOOD FILM TAKEN TAIL - BENT/MISSING BONE MARROW SMEAR TAKEN MALOCCLUSION TEETH MISSING/BROKEN/WORN DISCHARGE-OCULAR ANIMAL THIN STAINS-PERINEUM/PERIANAL FLUID-THORACIC CAVITY GI TRACT-LARGE LUMEN DISCHARGE-NASAL URINARY BLADDER-LARGE LUMEN ANIMAL OBESE DISCHARGE-ORAL AUTOLYSIS-SEVERE FLUID-ABDOMINAL CAVITY PALE MUCOUS MEMBRANES STAINS - SKIN/HAIRCOAT GI TRACT-DARK FLUID CONTENT GI TRACT-DARK DRY CONTENT YELLOWISH MUCOUS MEMBRANES TRACHEA-CONTENT FOAMY MATTER 19 6 9 9 11 0 8 10 9 8 10 34 39 39 38 38 29 47 46 44 40 45 000001 1 01 1 0 25 15 22 30 35 10 39 20 14 20 40 3 6 5 3 5 4 12 11 5 10 5 2521 34231 35 11 15 8 16 9 10 11 13 22 9 14 5 12 10 5 3 5 12 12 15 13 16 3422461 4723 01 001 1 0001 0 1 001 2 0001 01 33 061 1 2 331 6 2 1 02 3 01 1 000 93677421 21 1 1 4 01 01 01 1 00 00000000001 00002 1 00000 00001 000000 3 01 4 31 331 3 0 1 01 01 1 1 01 02 0000000001 0 0000000001 0 01 000000000 6 40 40 0 33 0 17 6 0 7 8 1 0 1 0 0 4 0 0 0 0 2 0 0 0 0 333 TABLE 34 Incidence of Macroscopic Observations All Animals 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 2 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;SUBSET=ALL ORGAN AND KEYWORD(S) OR PHRASE -- N U M B E R - O F - A N I M A L S - A F F E C T E D -- SEX: ---MALE--- --FEMALE--- GROUP: -1 - -2 - -3- -4- -5- -6- -1 - -2 - -3- -4- -5- -6- NUMBER: 70 60 60 60 70 40 70 60 60 60 70 40 EYE (EY) ..................... ........................ NUMBER EXAMINED: 70 60 60 60 70 40 70 60 60 60 70 40 NOT REMARKABLE: 70 59 59 60 70 40 70 60 59 60 69 39 OPAQUE CORNEA PROTRUDING LARGE WHITE INTRAOCULAR MATERIAL ROUGHENED CORNEA 001 000000001 001 00000001 0 001 000000000 01 0000000000 000000001 000 BRAIN (BR) ................... ........................ NUMBER EXAMINED: 70 60 60 60 70 40 70 60 60 60 70 40 NOT REMARKABLE: 45 38 27 36 51 19 32 24 18 22 39 17 COMPRESSED (HYPOTHALAMUS) MASS(ES) RED FOCUS(I)/AREA(S) DEPRESSED AREA(S) 25 20 33 24 19 21 38 36 42 37 31 23 01 0000000000 0000000001 00 01 0000000000 LIVER (LI) ................... ........................ NUMBER EXAMINED: 70 60 60 60 70 40 70 60 60 60 70 40 NOT REMARKABLE: 59 49 42 47 46 22 57 47 49 43 50 27 LARGE MOTTLED DIFFUSELY DARK HEPATIC ANOMALY (LOBULE) LIGHT FOCUS(I)/AREA(S) MASS(ES) ROUGH SURFACE, DIFFUSE 1 22 06 001 1 322 2 1 2 384 051 52 1 01 001 0001 05 1 001 000000001 0 6 6 4 10 1 3 0 1 5 6 1 002 1 2 1 01 02 3 0 1 0001 1 00031 0 334 TABLE 34 Incidence of Macroscopic Observations All Animals 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 3 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C T E D -- SEX: --MALE--- --FEMALE--- GROUP: -1 - -2 - -3- -4- -5- -6- -1 - -2 - -3- -4- -5- -6- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 70 60 60 60 70 40 70 60 60 60 70 40 -- -- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** LIVER (LI) ................................ ............ NUMBER EXAMINED: 70 60 60 60 70 40 70 60 60 60 70 40 NOT REMARKABLE: 59 49 42 47 46 22 57 47 49 43 50 27 DIFFUSELY LIGHT RED FOCUS(I)/AREA(S) CYST(S) DARK FOCUS(I)/AREA(S) RAISED FOCUS(I)/AREA(S) ADHESION(S) 1 231 1 51 1 1 41 2 5 2 6 5 2 8 12 6 7 4 4 7 00001 1 1 1 1 1 1 1 3 000000002 00 00000000003 0 000001 000000 KIDNEY (KD) ............................... ............ NUMBER EXAMINED: 70 60 60 60 70 40 70 60 60 60 70 40 NOT REMARKABLE: 63 48 51 49 54 27 62 54 53 53 66 35 CYST(S) LARGE PELVIS(ES) GRANULAR MATERIAL ROUGH SURFACE, DIFFUSE DIFFUSELY DARK MASS(ES) MOTTLED LARGE DEPRESSED FOCUS(I)/AREA(S) DIFFUSELY LIGHT LIGHT FOCUS(I)/AREA(S) SEMIFLUID MATERIAL-PELVIS SMALL 1 242 0331 1 1 00 2435863231 22 001 2 03 02 32 01 3 10 5 6 5 3 0 0 0 2 1 0 00001 01 1 02 1 1 00001 1 1 01 001 01 1 03 00002 00 22 01 31 0001 00 000001 000000 04 4 32 4 2 1 1 1 01 1 001 00000000 000001 000000 000001 000000 335 TABLE 34 Incidence of Macroscopic Observations All Animals 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T- 6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 4 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;SUBSET=ALL ORGAN AND KEYWORD(S) OR PHRASE N U M B E R - O F - A N I M A L S - A F F E C T E D -- SEX: --MALE--- --FEMALE--- GROUP: -1 - -2 - -3- -4- -5- -6- -1 - -2 - -3- -4- -5- -6- NUMBER: 70 60 60 60 70 40 70 60 60 60 70 40 SPLEEN (SP) .................. ........................ NUMBER EXAMINED: 70 60 60 60 70 40 70 60 60 60 70 40 NOT REMARKABLE: 67 59 59 60 66 34 66 55 59 56 62 38 LARGE SMALL CYST(S) DIFFUSELY LIGHT IRREGULAR SHAPE LIGHT FOCUS(I)/AREA(S) ADHESION(S) MASS(ES) MASS #2 MOTTLED 1 1 002 1 34 1 4 2 1 00002 1 00004 1 00000000002 0 001 001 01 0000 0000001 00000 1 000001 1 0000 2 00001 1 1 01 00 000002 000000 000001 000000 00000001 0000 PANCREAS (PA) ................ ........................ NUMBER EXAMINED: 70 60 60 60 70 40 70 60 60 60 70 40 NOT REMARKABLE: 68 58 56 57 67 36 69 60 58 60 69 40 MASS(ES) DIFFUSELY RED LARGE VESSELS GELATINOUS LIGHT FOCUS(I)/AREA(S) 1 2 2 1 32 1 02 000 0001 00000000 1 02 1 02 000000 00000000001 0 00000000001 0 ADRENAL, CORTEX (AC) ........ ........................ NUMBER EXAMINED: 70 60 60 60 70 40 70 60 60 60 70 40 NOT REMARKABLE: 68 52 57 58 65 37 52 41 49 43 56 32 LARGE 2 6 3 2 3 0 15 17 7 14 9 6 336 TABLE 34 Incidence of Macroscopic Observations All Animals 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 5 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C T E D -- SEX: ---MALE--- --FEMALE--- GROUP: -1 - -2 - -3- -4- -5- -6- -1 - -2 - -3- -4- -5- -6- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 70 60 60 60 70 40 70 60 60 60 70 40 -- ------------------------------------------- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** ADRENAL, CORTEX (AC) ........ ........................ NUMBER EXAMINED: 70 60 60 60 70 40 70 60 60 60 70 40 NOT REMARKABLE: 68 52 57 58 65 37 52 41 49 43 56 32 MASS(ES) CYST(S) DIFFUSELY RED SMALL DARK FOCUS(I)/AREA(S) RED FOCUS(I)/AREAS(I) MOTTLED IRREGULAR SHAPE MISSING/NOT IDENTIFIED DIFFUSELY DARK 00001 1 1 2 01 00 01 00001 001 00 000001 1 02 1 1 0 01 00001 01 02 2 0000001 01 1 01 00000001 1 2 3 0 1 000001 31 1 2 0 00000001 0000 000001 000000 00001 01 0002 0 ADRENAL, MEDULLA (MA) ....... ........................ NUMBER EXAMINED: 70 60 60 60 70 40 70 60 60 60 70 40 NOT REMARKABLE: 70 60 60 60 70 40 70 60 60 60 70 40 LN, MESENTERIC (MS) ................. ................. NUMBER EXAMINED: 70 60 60 60 70 40 70 60 60 60 70 40 NOT REMARKABLE: 70 58 58 58 68 40 68 56 59 56 67 39 LARGE DIFFUSELY RED MOTTLED DIFFUSELY DARK SMALL MASS(ES) 01 02 1 001 01 2 0 01 01 1 001 02 2 0 001 0000001 00 0000002 2 1 1 01 001 000000000 00001 0000000 337 TABLE 34 Incidence of Macroscopic Observations All Animals 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 6 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;SUBSET=ALL ORGAN AND KEYWORD(S) OR PHRASE -- N U M B E R - O F - A N I M A L S - A F F E C T E D -- SEX: ---MALE--- --FEMALE--- GROUP: -1- -2- -3- -4- -5- -6- -1- -2- -3- -4- -5- -6- NUMBER: 70 60 60 60 70 40 70 60 60 60 70 40 0 0 0 0 0 0UTERUS (UT) .................. ........................ NUMBER EXAMINED: 0 0 0 0 0 0NOT REMARKABLE: 70 60 60 60 70 40 57 47 45 41 57 30 LUMEN FILLED WITH FLUID THICKENED WALL CYST(S) DIFFUSELY RED MASS(ES) LARGE, DIFFUSE 0000 0000 0000 0000 0000 0000 22 90 1 38 0 23 22 19 110 23 6 0 1 5 40 0000001225 22 000000010001 TESTIS (TE) .................. ........................ NUMBER EXAMINED: 70 60 60 60 70 40 NOT REMARKABLE: 61 51 52 50 59 35 00 00 00 00 00 00 SMALL CONTAINS FLUID MASS(ES) RED/YELLOW FOCUS(I)/AREA(S) DIFFUSELY RED LARGE MOTTLED 85 5 9 83 000000 110 410 300 100 110 110 000 000 000 000 000 000 00 20 10 00 21 00 00 00 00 00 00 00 000010000000 BONE, FEMUR (FE) ............. ........................ NUMBER EXAMINED: 70 60 60 60 70 40 70 60 60 60 70 40 NOT REMARKABLE: 70 60 60 60 70 40 70 60 60 60 70 40 MARROW, FEMUR (FM) ................. .................. NUMBER EXAMINED: 70 60 60 60 70 40 70 60 60 60 70 40 NOT REMARKABLE: 70 60 60 60 70 40 70 60 60 60 70 40 338 TABLE 34 Incidence of Macroscopic Observations All Animals 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 7 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;SUBSET=ALL ORGAN AND KEYWORD(S) OR PHRASE -- N U M B E R - O F - ANI M A LS- A F F E C T E D - SEX: ---MALE--- --FEMALE--- GROUP: -1 - -2 - -3- -4- -5- -6- -1 - -2 - -3- -4- -5- -6 NUMBER: 70 60 60 60 70 40 70 60 60 60 70 40 MARROW, STERNUM (SE) ........ ........................ NUMBER EXAMINED: 70 60 60 60 70 40 70 60 60 60 70 40 NOT REMARKABLE: 70 60 60 60 70 40 70 60 60 60 70 40 BONE, STERNUM (SB) .......... ........................ NUMBER EXAMINED: 70 60 60 60 70 40 70 60 60 60 70 40 NOT REMARKABLE: 70 60 60 60 70 40 70 60 60 60 70 40 SPINAL CORD (SC) ............. ........................ NUMBER EXAMINED: 70 60 60 60 70 40 70 60 60 60 70 40 NOT REMARKABLE: 70 59 60 60 70 40 70 60 60 60 70 40 FRACTURE(S) 01 0000000000 THYMUS (TH) .................. ........................ NUMBER EXAMINED: 70 60 60 60 70 40 70 60 60 60 70 40 NOT REMARKABLE: 66 58 57 59 68 37 68 59 56 58 69 37 RED FOCUS(I)/AREA(S) LARGE MASS(ES) GELATINOUS MISSING/NOT IDENTIFIED 1 0001 1 002 001 00001 0000000 1 1 00001 00001 001 01 01 00000 2 1 2 1 02 01 2 2 1 1 LUNG (LU) .................... ........................ NUMBER EXAMINED: 70 60 60 60 70 40 70 60 60 60 70 40 NOT REMARKABLE: 66 57 58 57 67 34 68 56 56 55 55 35 MASS(ES) RED FOCUS(I)/AREA(S) 1 001 0001 02 001 2 0001 01 01 01 339 TABLE 34 Incidence of Macroscopic Observations All Animals 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 8 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C T E D -- SEX: --MALE--- --FEMALE--- GROUP: -1 - -2 - -3- -4- -5- -6- -1 - -2 - -3- -4- -5- -6- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 70 60 60 60 70 40 70 60 60 60 70 40 ---- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** LUNG (LU) .................... ........................ NUMBER EXAMINED: 70 60 60 60 70 40 70 60 60 60 70 40 NOT REMARKABLE: 66 57 58 57 67 34 68 56 56 55 55 35 LIGHT FOCUS(I)/AREA(S) DARK FOCUS(I)/AREA(S) DIFFUSELY RED MOTTLED UNCOLLAPSED DIFFUSELY LIGHT RAISED FOCUS(I)/AREA(S) ADHESION(S) 0 1 1 0 2 1 1 1 1 2 14 2 1 0001 0000000 1 000001 00000 1 1 01 02 001 000 01 0003 02 1 000 001 000000000 000000000001 0000000001 1 0 HEART (HT) ................... ........................ NUMBER EXAMINED: 70 60 60 60 70 40 70 60 60 60 70 40 NOT REMARKABLE: 70 57 58 60 70 37 70 60 60 59 70 40 LARGE DIFFUSELY LIGHT MASS(ES) LIGHT FOCUS(I)/AREA(S) 000001 0001 00 01 00000001 00 01 0000000000 01 2 002 000000 MUSCLE, SKELETAL (SM) ....... ........................ NUMBER EXAMINED: 70 60 60 60 70 40 70 60 60 60 70 40 NOT REMARKABLE: 68 60 59 59 69 40 70 60 60 59 69 40 MASS(ES) ATROPHY-DIFFUSE ATROPHY-FOCAL 2 01 1 1 0000000 00000000001 0 0000000001 00 340 TABLE 34 Incidence of Macroscopic Observations All Animals 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 9 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;SUBSET=ALL ORGAN AND KEYWORD(S) OR PHRASE -- N U M BE R -O F -A N IMA L S -A F FE CT E D - SEX: ---MALE --FEMALE--- GROUP: -1 - -2 - -3- -4- -5- -6- -1 - -2 - -3- -4- -5- -6 NUMBER: 70 60 60 60 70 40 70 60 60 60 70 40 NERVE, SCIATIC (SN) ................................... NUMBER EXAMINED: 70 60 60 60 70 40 70 60 60 60 70 40 NOT REMARKABLE: 70 60 60 60 70 40 70 60 60 60 70 40 TRACHEA (TR) .................... ..................... NUMBER EXAMINED: 70 60 60 60 70 40 70 60 60 60 70 40 NOT REMARKABLE: 70 60 60 60 70 40 70 60 60 60 70 40 ESOPHAGUS (ES) ..................... .................. NUMBER EXAMINED: 70 60 60 60 70 40 70 60 60 60 70 40 NOT REMARKABLE: 69 60 60 60 70 40 70 60 60 60 69 40 LARGE MASS(ES) 1 00000000000 00000000001 0 THYROID (TY) ....................... .................. NUMBER EXAMINED: 70 60 60 60 70 40 70 60 60 60 70 40 NOT REMARKABLE: 66 58 58 56 66 36 70 57 59 57 69 39 LARGE DIFFUSELY LIGHT MASS(ES) SMALL DIFFUSELY DARK DIFFUSELY RED 4 2 2 4 4 2 02 1 3 1 1 00000001 01 00 000002 01 0000 1 00000000000 1 00000000000 000000000001 341 TABLE 34 Incidence of Macroscopic Observations All Animals 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 10 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;SUBSET=ALL ORGAN AND KEYWORD(S) OR PHRASE -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---- SEX: ---MALE--- --FEMALE-- GROUP: -1 - -2 - -3- -4- -5- -6- -1 - -2 - -3- -4- -5- -6- NUMBER: 70 60 60 60 70 40 70 60 60 60 70 40 PARATHYROID (PT) ............. ........................ NUMBER EXAMINED: 70 60 60 60 70 40 70 60 60 60 70 40 NOT REMARKABLE: 70 59 58 60 68 39 70 59 60 60 70 39 LARGE 01 2 02 1 01 0001 PITUITARY (PI) ............... ........................ NUMBER EXAMINED: 70 60 60 60 70 40 70 60 60 60 70 40 NOT REMARKABLE: 38 30 18 29 38 10 20 14 14 17 23 8 LARGE DARK FOCUS(I)/AREA(S) MASS(ES) RED FOCUS(I)/AREA(S) RAISED AREA(S) CYST(S) DIFFUSELY RED MOTTLED LIGHT FOCUS(I)/AREA(S) 1 2 2 1 2 1 7 5 3 3 12 3 002 001 2 3 1 1 52 25 21 32 25 21 21 38 37 41 37 27 24 444454431 572 1 22 1 1 000001 1 001 032 001 003 1 00001 000000 00000001 1 000 1 1 0000000001 HARDERIAN GLAND (HG) ........ ........................ NUMBER EXAMINED: 70 60 60 60 70 40 70 60 60 60 70 40 NOT REMARKABLE: 70 59 58 60 70 40 70 60 60 60 69 40 MISSING/NOT IDENTIFIED MASS(ES) MOTTLED 01 1 000000000 00000000001 0 001 000000000 342 TABLE 34 Incidence of Macroscopic Observations All Animals 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 11 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;SUBSET=ALL ORGAN AND KEYWORD(S) OR PHRASE -- N U M B E R - O F - A N I M A L S - A F F E C T E D -- SEX: ---MALE--- --FEMALE--- GROUP: -1 - -2 - -3- -4- -5- -6- -1 - -2 - -3- -4- -5- -6- NUMBER: 70 60 60 60 70 40 70 60 60 60 70 40 SALIV GL, MANDIB (SG) .................... ............ NUMBER EXAMINED: 70 60 60 60 70 40 70 60 60 60 70 40 NOT REMARKABLE: 70 60 59 60 70 40 70 59 60 60 69 40 SMALL MISSING/NOT IDENTIFIED MASS(ES) DIFFUSELY LIGHT 00000000001 0 00000001 0000 001 000000000 001 000000000 STOMACH, GL (ST) ......................... ............ NUMBER EXAMINED: 70 60 60 60 70 40 70 60 60 60 70 40 NOT REMARKABLE: 57 41 44 47 50 23 62 54 56 57 61 33 DARK FOCUS(I)/AREA(S) EROSION/ULCERATION RED FOCUS(I)/AREA(S) THICKENED WALL LIGHT FOCUS(I)/AREA(S) RAISED AREA(S) 4 5 3 3 8 11 422385 2 1 31 2 1 2 1 0001 031 22 001 001 0 7 13 11 8 12 5 1 1 1 0 0 0 001 1 1 01 1 1 000 000000000001 STOMACH, NONGL (SU) ...................... ............ NUMBER EXAMINED: 70 60 60 60 70 40 70 60 60 60 70 40 NOT REMARKABLE: 62 56 57 53 65 35 68 55 56 58 67 39 EROSION/ULCERATION THICKENED WALL RED FOCUS(I)/AREA(S) LIGHT FOCUS(I)/AREA(S) DARK FOCUS(I)/AREA(S) 741 6452521 31 2 1 2 5 3 3 002 1 00 000001 001 000 002 0000001 00 0001 00000000 343 TABLE 34 Incidence of Macroscopic Observations All Animals 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T- 6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 12 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;SUBSET=ALL ORGAN AND KEYWORD(S) OR PHRASE N U M BE R -O F -A N IMA L S -A F FE CT E D - SEX: --MALE--- --FEMALE--- GROUP: -1 - -2 - -3- -4- -5- -6- -1 - -2 - -3- -4- -5- -6 NUMBER: 70 60 60 60 70 40 70 60 60 60 70 40 DUODENUM (DU) ................ ........................ NUMBER EXAMINED: 70 60 60 60 70 40 70 60 60 60 70 40 NOT REMARKABLE: 70 60 60 60 70 39 70 60 60 60 70 40 RED FOCUS(I)/AREA(S) 000001 000000 JEJUNUM (JE) ................. ........................ NUMBER EXAMINED: 70 60 60 60 70 40 70 60 60 60 70 40 NOT REMARKABLE: 69 60 60 60 70 40 70 60 60 60 69 39 MASS(ES) THICKENED WALL 00000000001 1 1 00000000000 ILEUM (IL) ................... ........................ NUMBER EXAMINED: 70 60 60 60 70 40 70 60 60 60 70 40 NOT REMARKABLE: 68 60 60 60 70 40 70 60 60 59 70 40 DIFFUSELY RED DIVERTICULUM(A) THICKENED WALL 1 00000000000 0000000001 00 1 00000000000 CECUM (CE) ................... ........................ NUMBER EXAMINED: 70 60 60 60 70 40 70 60 60 60 70 40 NOT REMARKABLE: 69 59 60 60 68 38 70 60 60 60 69 40 RED FOCUS(I)/AREA(S) DIFFUSELY RED THICKENED WALL LARGE MASS(ES) 00001 1 000000 1 1 0000000000 01 0001 000000 00000000001 0 00001 0000000 344 TABLE 34 Incidence of Macroscopic Observations All Animals 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 13 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;SUBSET=ALL ORGAN AND KEYWORD(S) OR PHRASE -- N U M B E R - O F - A N I M A L S - A F F E C T E D -- SEX: ---MALE--- --FEMALE--- GROUP: -1 - -2 - -3- -4- -5- -6- -1 - -2 - -3- -4- -5- -6- NUMBER: 70 60 60 60 70 40 70 60 60 60 70 40 COLON (CO) ................................ ............ NUMBER EXAMINED: 70 60 60 60 70 40 70 60 60 60 70 40 NOT REMARKABLE: 70 60 60 60 70 40 69 59 60 60 70 40 MASS(ES) DIVERTICULUM(A) 00000001 0000 0000001 00000 RECTUM (RE) ............................... ............ NUMBER EXAMINED: 70 60 60 60 70 40 70 60 60 60 70 40 NOT REMARKABLE: 69 60 60 60 70 40 70 60 59 60 70 40 MASS(ES) DIFFUSELY RED 000000001 000 1 00000000000 SKIN (SK) ................................. ............ NUMBER EXAMINED: 70 60 60 60 70 40 70 60 60 60 70 40 NOT REMARKABLE: 64 49 52 52 57 33 63 59 57 58 66 37 ABRASION(S)/ULCERATION(S) CRUSTED AREA(S) LIGHT AREA(S)/FOCUS(I) MASS(ES) RED FOCUS(I)/AREA(S) RAISED FOCUS(I)/AREA(S) ALOPECIA-DIFFUSE THICKENED MASS #2 SUBCUTANEOUS EDEMA 01 002 01 0001 0 35245631 1 1 32 0000001 00000 374 371 1 01 01 0 1 00000000000 1 02 2 1 01 00001 0000001 001 01 000000001 000 02 0000000000 00001 0000000 345 TABLE 34 Incidence of Macroscopic Observations All Animals 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T- 6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 14 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;SUBSET=ALL ORGAN AND KEYWORD(S) OR PHRASE N U M BE R -O F -A N IMA L S -A F FE CT E SEX: --MALE--- --FEMALE--- GROUP: -1 - -2 - -3- -4- -5- -6- -1 - -2 - -3- -4- -5- NUMBER: 70 60 60 60 70 40 70 60 60 60 70 6 40 MAMMARY, FEMALE (MF) ........ ........................ NUMBER EXAMINED: 0 0 0 0 0 0 70 60 60 60 70 40 NOT REMARKABLE: 0 0 0 0 0 0 29 23 22 24 39 20 MASS(ES) THICKENED MASS #2 CYST(S) MASS #3 MASS #4 MASS #5 MASS #6 MASS #7 MASS #8 0 0 0 0 0 0 28 35 29 28 24 17 0 0 0 0 0 0 15 10 16 13 9 5 0 0 0 0 0 0 14 13 11 14 10 7 0 0 0 0 0 0 13 5 9 4 5 3 0000006 5 3 6 2 2 0000002 1 2 1 2 2 00000001 2 1 1 0 00000000001 0 00000000001 0 00000000001 0 URINARY BLADDER (UB) ........ ........................ NUMBER EXAMINED: 70 60 60 60 70 40 70 60 60 60 70 40 NOT REMARKABLE: 67 58 59 57 68 39 70 60 60 60 70 40 CALCULUS(I) DIFFUSELY RED SEMIFLUID MATERIAL THICKENED WALL RED FOCUS(I)/AREA(S) 2 001 1 1 000000 1 002 00000000 02 1 000000000 01 02 00000000 00001 0000000 CERVIX (CV) .................. ........................ NUMBER EXAMINED: 0 0 0 0 0 0 70 60 60 60 70 40 NOT REMARKABLE: 0 0 0 0 0 0 68 57 56 58 66 39 MASS(ES) 0000001 2 002 1 346 TABLE 34 Incidence of Macroscopic Observations All Animals 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 15 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---SEX: ------------ MALE------------ ----------- FEMALE GROUP: -1 - -2 - -3- -4- -5- -6- -1 - -2 - -3- -4- -5- -6- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 70 60 60 60 70 40 70 60 60 60 70 40 -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=** FROM PREVIOUS PAGE ** CERVIX (CV) .................. ........................ NUMBER EXAMINED: 0 0 0 0 0 0 70 60 60 60 70 40 NOT REMARKABLE: 0 0 0 0 0 0 68 57 56 58 66 39 FIRM LARGE CYST(S) 0000001 00000 0000001 1 4 1 2 0 0000000001 00 OVARY (OV) ................... ........................ NUMBER EXAMINED: 0 0 0 0 0 0 70 60 60 60 70 40 NOT REMARKABLE: 0 0 0 0 0 0 58 55 51 47 56 29 CYST(S) MASS(ES) LARGE 0 0 0 0 0 0 12 5 9 13 12 11 00000000001 0 00000000001 0 VAGINA (VA) .................. ........................ NUMBER EXAMINED: 0 0 0 0 0 0 70 60 60 60 70 40 NOT REMARKABLE: 0 0 0 0 0 0 65 54 53 54 66 35 SEMIFLUID MATERIAL MASS(ES) 000000564 54 5 0000000031 00 PROSTATE (PR) ................ ........................ NUMBER EXAMINED: 70 60 60 60 70 40 0 0 0 0 0 0 NOT REMARKABLE: 56 51 44 51 58 33 0 0 0 0 0 0 NODULAR SMALL MASS(ES) LARGE 11 7 12 6 10 6 0 0 0 0 0 0 21 1 1 1 0000000 1 01 1 00000000 01 4 003 000000 347 TABLE 34 Incidence of Macroscopic Observations All Animals 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T- 6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 16 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;SUBSET=ALL N U M B E R - O F - A N I M A L S - A F F E C T E D -- SEX: --MALE--- --FEMALE--- GROUP: -1 - -2 - -3- -4- -5- -6- -1 - -2 - -3- -4- -5- -6- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 70 60 60 60 70 40 70 60 60 60 70 40 ** FROM PREVIOUS PAGE ** PROSTATE (PR) ................ ........................ NUMBER EXAMINED: 70 60 60 60 70 40 0 0 0 0 0 0 NOT REMARKABLE: 56 51 44 51 58 33 0 0 0 0 0 0 GELATINOUS 002 1 1 1 000000 SEMINAL VESICLES (SV) ....... ........................ NUMBER EXAMINED: 70 60 60 60 70 40 0 0 0 0 0 0 NOT REMARKABLE: 64 49 49 52 62 33 0 0 0 0 0 0 SMALL LARGE GELATINOUS THICKENED MOTTLED 563551 000000 1 3631 4 000000 02 3 1 2 4 000000 01 0000000000 0001 00000000 EPIDIDYMIDES (EP) ............ ........................ NUMBER EXAMINED: 70 60 60 60 70 40 0 0 0 0 0 0 NOT REMARKABLE: 70 60 60 60 68 40 0 0 0 0 0 0 SMALL 00002 0000000 CAVITY, THORACIC (TA) ....... ........................ NUMBER EXAMINED: 70 60 60 60 70 40 70 60 60 60 70 40 NOT REMARKABLE: 70 60 60 60 70 40 69 60 60 60 70 40 MASS(ES) 0000001 00000 348 TABLE 34 Incidence of Macroscopic Observations All Animals 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 17 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;SUBSET=ALL ORGAN AND KEYWORD(S) OR PHRASE -- N U M B E R - O F - A N I M A L S - A F F E C T E D -- SEX: ---MALE--- --FEMALE--- GROUP: -1 - -2 - -3- -4- -5- -6- -1 - -2 - -3- -4- -5- -6- NUMBER: 70 60 60 60 70 40 70 60 60 60 70 40 TAIL (TI) .................... ........................ NUMBER EXAMINED: 70 60 60 60 70 40 70 60 60 60 70 40 NOT REMARKABLE: 65 60 56 58 70 38 69 59 59 59 70 40 ABRASION(S)/ULCERATION(S) CRUSTED AREA(S) LARGE DIFFUSELY RED RAISED AREA(S) MASS(ES) 000000001 000 1 01 1 02 01 0000 1 01 000000000 0000001 00000 2 01 1 00000000 1 01 0000001 00 LN, MANDIBULAR (MN) ......... ........................ NUMBER EXAMINED: 70 60 60 60 70 40 70 60 60 60 70 40 NOT REMARKABLE: 65 55 55 58 67 36 58 56 53 56 64 33 DIFFUSELY RED LARGE MOTTLED 1 4422481 1 246 1 1 1 01 0334 1 32 3 000002 03 1 00 LN, MEDIASTINAL (ML) ........ ........................ NUMBER EXAMINED: 70 60 60 60 70 40 70 60 60 60 70 40 NOT REMARKABLE: 70 58 58 58 67 37 68 60 58 59 67 39 LARGE DIFFUSELY RED MOTTLED CYST(S) 01 2 1 33 001 03 0 01 1 02 2 2 02 1 1 1 001 000000000 0001 00000000 349 TABLE 34 Incidence of Macroscopic Observations All Animals 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 18 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---SEX: ------------ MALE------------ ----------- FEMALE GROUP: -1 - -2 - -3- -4- -5- -6- -1 - -2 - -3- -4- -5- -6- ORGAN AND KEYWORD(S) OR PHRASE ---- NUMBER: 70 60 60 60 70 40 70 60 60 60 70 40 -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- LN, AXILLARY (AX) ............ ........................ NUMBER EXAMINED: 70 60 60 60 70 40 70 60 60 60 70 40 NOT REMARKABLE: 69 57 58 58 67 39 66 53 56 54 68 34 LARGE MOTTLED DIFFUSELY RED DIFFUSELY DARK 01 1 1 002 3 34 2 5 1 1 001 004 1 2 02 01 222 1 1 001 1 2 0000001 00000 LN, INGUINAL (IN) ............ ........................ NUMBER EXAMINED: 70 60 60 60 70 40 70 60 60 60 70 40 NOT REMARKABLE: 70 59 60 60 70 40 70 58 57 59 69 40 LARGE DIFFUSELY RED 01 000002 3 1 00 00000000001 0 LN, OTHER (LN) ............... ........................ NUMBER EXAMINED: 70 60 60 60 70 40 70 60 60 60 70 40 NOT REMARKABLE: 69 59 59 59 70 40 70 60 60 60 70 40 LARGE DIFFUSELY RED 1 1 01 00000000 001 000000000 LN, RENAL (RL) ............... ........................ NUMBER EXAMINED: 70 60 60 60 70 40 70 60 60 60 70 40 NOT REMARKABLE: 70 56 57 57 66 37 69 60 60 59 69 40 LARGE DIFFUSELY RED CYST(S) MOTTLED 02 2 32 3 0001 1 0 032 1 3 00001 1 0 000001 000000 000001 1 00000 350 TABLE 34 Incidence of Macroscopic Observations All Animals 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 19 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C T E D -- SEX: ---MALE--- --FEMALE--- GROUP: -1- -2- -3- -4- -5- -6- -1- -2- -3- -4- -5- -6- ORGAN AND KEYWORD(S) OR PHRASE -------------------------------- -- NUMBER: 70 60 60 60 70 40 70 60 60 60 70 40 -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- MAMMARY, MALE (MM) .......... ........................ NUMBER EXAMINED: 70 60 60 60 70 40 NOT REMARKABLE: 64 51 54 54 65 37 00 00 00 00 00 00 THICKENED CYST(S) MASS(ES) MASS #2 010100000000 1 25 4 40 42 0 0 50 1 25 221 000 000 000 000 000 000 COAGULATING GL (CG) ......... ........................ NUMBER EXAMINED: 70 60 60 60 70 40 NOT REMARKABLE: 70 60 60 59 70 40 00 00 00 00 00 00 LARGE 000100000000 LN, ANT MES/PANC (AP) ....... ........................ NUMBER EXAMINED: 70 60 60 60 70 40 70 60 60 60 70 40 68NOT REMARKABLE: 70 59 58 59 70 37 69 59 58 60 40 LARGE DIFFUSELY RED MOTTLED 001 002 011 01 0 00 10 10 10 00 30 10 10 10 00 20 00 PREPUTIAL GLAND (PG) ........ ........................ NUMBER EXAMINED: 70 60 60 60 70 40 NOT REMARKABLE: 70 59 58 60 69 38 00 00 00 00 00 00 LARGE 012012000000 351 TABLE 34 Incidence of Macroscopic Observations All Animals 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T- 6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 20 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;SUBSET=ALL N U M B E R - O F - A N I M A L S - A F F E C T E D -- SEX: ---MALE-- --FEMALE--- GROUP: -1 - -2 - -3- -4- -5- -6- -1 - -2 - -3- -4- -5- -6- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 70 60 60 60 70 40 70 60 60 60 70 40 -------------------------------- ------------------------------------------- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- CAVITY, ABDOM (PC) .......... ........................ NUMBER EXAMINED: 70 60 60 60 70 40 70 60 60 60 70 40 NOT REMARKABLE: 69 60 60 57 68 36 68 60 60 60 67 39 MASS(ES) LARGE VESSELS 1 002 2 2 2 0003 1 0001 02 000000 AORTA (AO) ................... ........................ NUMBER EXAMINED: 70 60 60 60 70 40 70 60 60 60 70 40 NOT REMARKABLE: 70 59 57 58 68 39 70 60 60 59 70 40 MASS(ES) LARGE FIRM 00001 0000000 01 31 1 1 0001 00 01 2 1 01 0001 00 FOOT/FOOTPAD (FP) ............ ........................ NUMBER EXAMINED: 70 60 60 60 70 40 70 60 60 60 70 40 NOT REMARKABLE: 68 58 57 54 66 38 68 57 58 58 65 38 CRUSTED AREA(S) ABRASION(S)/ULCERATION(S) LARGE 001 4 32 1 2 1 1 2 1 1 222 1 01 1 1 1 31 1 00000000000 AUDITORY SEB GL (AS) ........ ........................ NUMBER EXAMINED: 70 60 60 60 70 40 70 60 60 60 70 40 NOT REMARKABLE: 70 59 59 60 70 40 70 60 60 60 70 40 MASS(ES) 01 1 000000000 352 TABLE 34 Incidence of Macroscopic Observations All Animals 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T- 6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 21 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;SUBSET=ALL ORGAN AND KEYWORD(S) OR PHRASE -- N U M B E R - O F - A N I M A L S - A F F E C T E D - SEX: ---MALE--- --FEMALE--- GROUP: -1 - -2 - -3- -4- -5- -6- -1 - -2 - -3- -4- -5- -6 NUMBER: 70 60 60 60 70 40 70 60 60 60 70 4:0 CALVARIUM/SKULL (CA) ........ ........................ NUMBER EXAMINED: 70 60 60 60 70 40 70 60 60 60 70 4:0 NOT REMARKABLE: 70 59 59 60 70 40 70 60 59 60 70 4:0 MASS(ES) 01 1 000001 000 LN, LUMBAR/ILIAC (LM) ....... ........................ NUMBER EXAMINED: 70 60 60 60 70 40 70 60 60 60 70 4:0 NOT REMARKABLE: 68 59 57 54 68 34 69 60 56 58 69 38 CYST(S) LARGE DIFFUSELY RED MASS(ES) MOTTLED 01 01 01 001 001 1 034 1 3 001 1 02 1 01 001 002 1 1 0 000000001 000 0001 1 1 1 01 1 00 URETER (UE) .................. ........................ NUMBER EXAMINED: 70 60 60 60 70 40 70 60 60 60 70 40 NOT REMARKABLE: 70 60 60 60 70 39 69 60 60 59 70 40 LARGE 000001 1 001 00 JOINT, OTHER (JT) ............ ........................ NUMBER EXAMINED: 70 60 60 60 70 40 70 60 60 60 70 40 NOT REMARKABLE: 69 59 57 59 69 37 70 60 60 60 69 40 LARGE 1 1 31 1 3 00001 0 353 TABLE 34 Incidence of Macroscopic Observations All Animals 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 22 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C T E D -- SEX: ---MALE--- --FEMALE--- GROUP: -1 - -2 - -3- -4- -5- -6- -1 - -2 - -3- -4- -5- -6- ORGAN AND KEYWORD(S) OR PHRASE ---- NUMBER: 70 60 60 60 70 40 70 60 60 60 70 40 -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ADIPOSE TISSUE (AT) ......... ........................ NUMBER EXAMINED: 70 60 60 60 70 40 70 60 60 60 70 40 NOT REMARKABLE: 69 60 58 60 70 40 69 58 60 60 70 40 MASS(ES) CYST(S) THICKENED GELATINOUS 1 01 000000000 0000001 00000 001 00001 0000 00000001 0000 VAS DEFERENS (VS) ............ ........................ NUMBER EXAMINED: 70 60 60 60 70 40 0 0 0 0 0 0 NOT REMARKABLE: 70 60 59 60 70 39 0 0 0 0 0 0 LARGE 001 001 000000 DIAPHRAGM (DP) ............... ........................ NUMBER EXAMINED: 70 60 60 60 70 40 70 60 60 60 70 40 NOT REMARKABLE: 70 60 60 60 70 40 69 60 60 60 70 40 MASS(ES) 0000001 00000 LN, POPLITEAL (PP) .......... ........................ NUMBER EXAMINED: 70 60 60 60 70 40 70 60 60 60 70 40 NOT REMARKABLE: 70 60 60 60 70 39 70 60 59 60 70 39 LARGE DIFFUSELY RED 000001 001 000000001 001 001 354 TABLE 34 Incidence of Macroscopic Observations All Animals 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 23 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---SEX: ------------ MALE------------ ----------- FEMALE GROUP: -1 - -2 - -3- -4- -5- -6- -1 - -2 - -3- -4- -5- -6- ORGAN AND KEYWORD(S) OR PHRASE ---- NUMBER: 70 60 60 60 70 40 70 60 60 60 70 40 -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- PERICARDIAL SAC (PD) ........ ........................ NUMBER EXAMINED: 70 60 60 60 70 40 70 60 60 60 70 40 NOT REMARKABLE: 70 60 59 60 70 39 70 60 60 60 70 40 MASS(ES) 001 001 000000 ARTERY, OTHER (AU) .......... ........................ NUMBER EXAMINED: 70 60 60 60 70 40 70 60 60 60 70 40 NOT REMARKABLE: 70 60 59 60 70 40 70 60 60 60 70 40 LARGE 001 000000000 AORTIC ARCH (AA) ............. ........................ NUMBER EXAMINED: 70 60 60 60 70 40 70 60 60 60 70 40 NOT REMARKABLE: 70 60 59 60 70 40 70 60 60 60 70 40 LARGE 001 000000000 LN, ILEOCECAL (IC) .......... ........................ NUMBER EXAMINED: 70 60 60 60 70 40 70 60 60 60 70 40 NOT REMARKABLE: 70 60 60 60 70 40 70 60 60 59 70 40 LARGE DIFFUSELY RED 0000000001 0000000001 00 00 LN, TRACHEOBRON (TB) ........ ........................ NUMBER EXAMINED: 70 60 60 60 70 40 70 60 60 60 70 40 NOT REMARKABLE: 70 60 60 60 70 39 70 60 60 60 70 40 LARGE DIFFUSELY RED 000001 000001 000000 000000 355 TABLE 34 Incidence of Macroscopic Observations All Animals 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 24 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---SEX: ------------ MALE------------ ----------- FEMALE GROUP: -1 - -2 - -3- -4- -5- -6- -1 - -2 - -3- -4- -5- -6- ORGAN AND KEYWORD(S) OR PHRASE -------------------------------- -- NUMBER: 70 60 60 60 70 40 70 60 60 60 70 40 -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- MUSCLE, OTHER (MO) .......... ........................ NUMBER EXAMINED: 70 60 60 60 70 40 70 60 60 60 70 40 NOT REMARKABLE: 70 60 59 60 70 40 70 60 60 60 70 40 THICKENED DIFFUSELY RED 001 001 000000000 000000000 PINNA (PN) ................... ........................ NUMBER EXAMINED: 70 60 60 60 70 40 70 60 60 60 70 40 NOT REMARKABLE: 70 60 60 60 70 39 70 60 60 60 70 39 MASS(ES) 000001 000001 BILE DUCT (BD) ............... ........................ NUMBER EXAMINED: 70 60 60 60 70 40 70 60 60 60 70 40 NOT REMARKABLE: 70 60 59 60 70 40 70 60 60 60 70 40 LARGE 001 000000000 TRIGEMINAL NERVE (TG) ....... ........................ NUMBER EXAMINED: 70 60 60 60 70 40 70 60 60 60 70 40 NOT REMARKABLE: 70 60 60 59 70 40 70 60 60 60 70 40 LARGE 0001 00000000 CLITORAL GLAND (CL) ......... ........................ NUMBER EXAMINED: 0 0 0 0 0 0 70 60 60 60 70 40 NOT REMARKABLE: 0 0 0 0 0 0 69 60 60 60 70 40 MASS(ES) ** END OF LIST ** 0000001 00000 356 TABLE 35 Incidence of Macroscopic Observations Recovery Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 1 TABLE INCLUDES: SEX=ALL;GROUP=6 ;WEEKS=ALL DEATH=U;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---SEX: MALE FEMALE GROUP: -6- -6- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 10 17 ** TOP OF LIST ** GENERAL COMMENT (GC) ..................... ............ NUMBER EXAMINED: 10 17 BLOOD FILM TAKEN TAIL - BENT/MISSING BONE MARROW SMEAR TAKEN MALOCCLUSION TEETH MISSING/BROKEN/WORN DISCHARGE-OCULAR ANIMAL THIN ANIMAL OBESE GI TRACT-DARK FLUID CONTENT 10 17 10 10 17 23 20 11 11 12 10 EYE (EY) .................................. ............ NUMBER EXAMINED: 10 NOT REMARKABLE: 10 17 16 OPAQUE CORNEA 01 BRAIN (BR) ................................ ............ NUMBER EXAMINED: 10 17 NOT REMARKABLE: 6 10 COMPRESSED (HYPOTHALAMUS) 47 LIVER (LI) ................................ ............ NUMBER EXAMINED: 10 17 NOT REMARKABLE: 6 10 LIGHT FOCUS(I)/AREA(S) ** CONTINUED ON NEXT PAGE ** 01 357 TABLE 35 Incidence of Macroscopic Observations Recovery Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 2 TABLE INCLUDES: SEX=ALL;GROUP=6 ;WEEKS=ALL DEATH=U;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---SEX: MALE FEMALE GROUP: -6- -6- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 10 17 ** FROM PREVIOUS PAGE ** LIVER (LI) ................................ ............ NUMBER EXAMINED: 10 NOT REMARKABLE: 6 17 10 RED FOCUS(I)/AREA(S) CYST(S) 46 01 KIDNEY (KD) ............................... ............ NUMBER EXAMINED: 10 17 NOT REMARKABLE: 7 16 CYST(S) LARGE PELVIS(ES) ROUGH SURFACE, DIFFUSE DIFFUSELY LIGHT 20 11 10 10 SPLEEN (SP) ............................... ............ NUMBER EXAMINED: 10 17 NOT REMARKABLE: 9 17 ADHESION(S) 10 PANCREAS (PA) ............................. ............ NUMBER EXAMINED: 10 17 NOT REMARKABLE: 8 17 MASS(ES) LARGE VESSELS 10 10 358 TABLE 35 Incidence of Macroscopic Observations Recovery Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 3 TABLE INCLUDES: SEX=ALL;GROUP=6 ;WEEKS=ALL DEATH=U;SUBSET=ALL ORGAN AND KEYWORD(S) OR PHRASE -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---SEX: MALE FEMALE GROUP: -6- -6NUMBER: 10 17 ADRENAL, CORTEX (AC) ..................... ............ NUMBER EXAMINED: NOT REMARKABLE: LARGE ADRENAL, MEDULLA (MA) .................... ............ NUMBER EXAMINED: NOT REMARKABLE: 10 10 0 10 10 17 13 4 17 17 LN, MESENTERIC (MS) ...................... ............ NUMBER EXAMINED: NOT REMARKABLE: DIFFUSELY DARK UTERUS (UT) ............................... ............ NUMBER EXAMINED: NOT REMARKABLE: THICKENED WALL CYST(S) MASS(ES) TESTIS (TE) ............................... ............ NUMBER EXAMINED: NOT REMARKABLE: SMALL 10 10 0 0 0 0 0 0 10 9 1 17 16 1 17 11 3 3 2 0 0 0 359 TABLE 35 Incidence of Macroscopic Observations Recovery Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 4 TABLE INCLUDES: SEX=ALL;GROUP=6 ;WEEKS=ALL DEATH=U;SUBSET=ALL ORGAN AND KEYWORD(S) OR PHRASE -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---SEX: MALE FEMALE GROUP: -6- -6NUMBER: 10 17 BONE, FEMUR (FE) ......................... ............ NUMBER EXAMINED: 10 NOT REMARKABLE: 10 17 17 MARROW, FEMUR (FM) ....................... ............ NUMBER EXAMINED: 10 NOT REMARKABLE: 10 17 17 MARROW, STERNUM (SE) ..................... ............ NUMBER EXAMINED: 10 NOT REMARKABLE: 10 17 17 BONE, STERNUM (SB) ....................... ............ NUMBER EXAMINED: 10 NOT REMARKABLE: 10 17 17 SPINAL CORD (SC) ......................... ............ NUMBER EXAMINED: 10 NOT REMARKABLE: 10 17 17 THYMUS (TH) ............................... ............ NUMBER EXAMINED: NOT REMARKABLE: MASS(ES) MISSING/NOT IDENTIFIED 10 9 0 1 17 15 1 1 360 TABLE 35 Incidence of Macroscopic Observations Recovery Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 5 TABLE INCLUDES: SEX=ALL;GROUP=6 ;WEEKS=ALL DEATH=U;SUBSET=ALL ORGAN AND KEYWORD(S) OR PHRASE -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---SEX: MALE FEMALE GROUP: -6- -6NUMBER: 10 17 LUNG (LU) ................................. ............ NUMBER EXAMINED: NOT REMARKABLE: RED FOCUS(I)/AREA(S) LIGHT FOCUS(I)/AREA(S) MOTTLED HEART (HT) ................................ ............ NUMBER EXAMINED: NOT REMARKABLE: 10 8 0 1 1 10 10 17 15 1 1 0 17 17 MUSCLE, SKELETAL (SM) .................... ............ NUMBER EXAMINED: 10 NOT REMARKABLE: 10 17 17 NERVE, SCIATIC (SN) ...................... ............ NUMBER EXAMINED: 10 NOT REMARKABLE: 10 17 17 TRACHEA (TR) .............................. ............ NUMBER EXAMINED: 10 NOT REMARKABLE: 10 17 17 ESOPHAGUS (ES) ............................ ............ NUMBER EXAMINED: 10 NOT REMARKABLE: 10 17 17 361 TABLE 35 Incidence of Macroscopic Observations Recovery Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 6 TABLE INCLUDES: SEX=ALL;GROUP=6 ;WEEKS=ALL DEATH=U;SUBSET=ALL ORGAN AND KEYWORD(S) OR PHRASE -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---SEX: MALE FEMALE GROUP: -6 -6 NUMBER: 10 17 THYROID (TY) .............................. ............ NUMBER EXAMINED: NOT REMARKABLE: LARGE MASS(ES) DIFFUSELY RED PARATHYROID (PT) ......................... ............ NUMBER EXAMINED: NOT REMARKABLE: LARGE PITUITARY (PI) ............................ ............ NUMBER EXAMINED: NOT REMARKABLE: LARGE DARK FOCUS(I)/AREA(S) MASS(ES) RED FOCUS(I)/AREA(S) CYST(S) LIGHT FOCUS(I)/AREA(S) HARDERIAN GLAND (HG) ..................... ............ NUMBER EXAMINED: NOT REMARKABLE: 10 8 1 1 0 10 10 0 10 2 0 1 4 2 1 0 10 10 17 16 1 0 1 17 16 1 17 6 2 2 7 1 1 1 17 17 362 TABLE 35 Incidence of Macroscopic Observations Recovery Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 7 TABLE INCLUDES: SEX=ALL;GROUP=6 ;WEEKS=ALL DEATH=U;SUBSET=ALL ORGAN AND KEYWORD(S) OR PHRASE -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---SEX: MALE FEMALE GROUP: -6- -6NUMBER: 10 17 SALIV GL, MANDIB (SG) .................... ............ NUMBER EXAMINED: 10 NOT REMARKABLE: 10 17 17 STOMACH, GL (ST) ......................... ............ NUMBER EXAMINED: NOT REMARKABLE: DARK FOCUS(I)/AREA(S) EROSION/ULCERATION THICKENED WALL STOMACH, NONGL (SU) ...................... ............ NUMBER EXAMINED: NOT REMARKABLE: 10 6 1 1 2 10 10 17 13 3 1 0 17 17 DUODENUM (DU) ............................. ............ NUMBER EXAMINED: 10 NOT REMARKABLE: 10 17 17 JEJUNUM (JE) .............................. ............ NUMBER EXAMINED: NOT REMARKABLE: MASS(ES) ILEUM (IL) ................................ ............ NUMBER EXAMINED: NOT REMARKABLE: 10 10 0 10 10 17 16 1 17 17 363 TABLE 35 Incidence of Macroscopic Observations Recovery Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 8 TABLE INCLUDES: SEX=ALL;GROUP=6;WEEKS=ALL DEATH=U;SUBSET=ALL ORGAN AND KEYWORD(S) OR PHRASE -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---SEX: MALE FEMALE GROUP: -6- -6- NUMBER: 10 17 10CECUM (CE) ................................ ............ NUMBER EXAMINED: 17 NOT REMARKABLE: 9 17 THICKENED WALL 10 10COLON (CO) ................................ ............ NUMBER EXAMINED: 10NOT REMARKABLE: 17 17 10RECTUM (RE) ............................... ............ NUMBER EXAMINED: 10NOT REMARKABLE: 17 17 10SKIN (SK) ................................. ............ NUMBER EXAMINED: 17 NOT REMARKABLE: 7 15 CRUSTED AREA(S) MASS(ES) RAISED FOCUS(I)/AREA(S) 210 110 MAMMARY, FEMALE (MF) ..................... ............ NUMBER EXAMINED: NOT REMARKABLE: 0 17 09 MASS(ES) THICKENED MASS #2 MASS #3 ** CONTINUED ON NEXT PAGE ** 08 000 3 41 364 TABLE 35 Incidence of Macroscopic Observations Recovery Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 9 TABLE INCLUDES: SEX=ALL;GROUP=6 ;WEEKS=ALL DEATH=U;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---SEX: MALE FEMALE GROUP: -6- -6- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 10 17 ** FROM PREVIOUS PAGE ** MAMMARY , FEMALE (MF) ............. .................... NUMBER EXAMINED: NOT REMARKABLE: 0 17 09 MASS #4 01 URINARY BLADDER (UB) ............. .................... NUMBER EXAMINED: 10 NOT REMARKABLE: 10 17 17 CERVIX (CV) ...................... .................... NUMBER EXAMINED: NOT REMARKABLE: MASS(ES) OVARY (OV) ....................... .................... NUMBER EXAMINED: NOT REMARKABLE: CYST(S) VAGINA (VA) ...................... .................... NUMBER EXAMINED: NOT REMARKABLE: 0 17 0 16 01 0 17 0 10 07 0 17 0 17 PROSTATE (PR) .................... .................... NUMBER EXAMINED: NOT REMARKABLE: NODULAR 10 9 1 0 0 0 365 TABLE 35 Incidence of Macroscopic Observations Recovery Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 10 TABLE INCLUDES: SEX=ALL;GROUP=6 ;WEEKS=ALL DEATH=U;SUBSET=ALL ORGAN AND KEYWORD(S) OR PHRASE -- N U M B E R - O F - A N I M A L S - A F F E C SEX: MALE FEMALE GROUP: -6- -6NUMBER: 10 17 T E D -- SEMINAL VESICLES (SV) .................... ............ NUMBER EXAMINED: NOT REMARKABLE: SMALL GELATINOUS EPIDIDYMIDES (EP) ........................ ............ NUMBER EXAMINED: NOT REMARKABLE: 10 9 1 1 10 10 0 0 0 0 0 0 CAVITY, THORACIC (TA) .................... ............ NUMBER EXAMINED: 10 NOT REMARKABLE: 10 17 17 TAIL (TI) ................................. ............ NUMBER EXAMINED: NOT REMARKABLE: CRUSTED AREA(S) LN, MANDIBULAR (MN) ...................... ............ NUMBER EXAMINED: NOT REMARKABLE: DIFFUSELY RED LARGE 10 8 2 10 9 1 0 17 17 0 17 12 4 1 366 TABLE 35 Incidence of Macroscopic Observations Recovery Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 11 TABLE INCLUDES: SEX=ALL;GROUP=6 ;WEEKS=ALL DEATH=U;SUBSET=ALL ORGAN AND KEYWORD(S) OR PHRASE -- N U M B E R - O F - A N I M A L S - A F F E C SEX: MALE FEMALE GROUP: -6- -6NUMBER: 10 17 T E D -- LN, MEDIASTINAL (ML) ..................... ............ NUMBER EXAMINED: NOT REMARKABLE: LARGE DIFFUSELY RED LN, AXILLARY (AX) ........................ ............ NUMBER EXAMINED: NOT REMARKABLE: LARGE LN, INGUINAL (IN) ........................ ............ NUMBER EXAMINED: NOT REMARKABLE: 10 9 1 0 10 10 0 10 10 17 16 0 1 17 16 1 17 17 LN, OTHER (LN) ............................ ............ NUMBER EXAMINED: 10 NOT REMARKABLE: 10 17 17 LN, RENAL (RL) ............................ ............ NUMBER EXAMINED: NOT REMARKABLE: LARGE MOTTLED MAMMARY, MALE (MM) ....................... ............ NUMBER EXAMINED: NOT REMARKABLE: 10 9 1 1 10 10 17 17 0 0 0 0 367 TABLE 35 Incidence of Macroscopic Observations Recovery Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 12 TABLE INCLUDES: SEX=ALL;GROUP=6 ;WEEKS=ALL DEATH=U;SUBSET=ALL ORGAN AND KEYWORD(S) OR PHRASE -- N U M B E R - O F - A N I M A L S - A F F E C SEX: MALE FEMALE GROUP: -6 -6 NUMBER: 10 17 T E D -- COAGULATING GL (CG) ...................... ............ NUMBER EXAMINED: 10 NOT REMARKABLE: 10 0 0 LN, ANT MES/PANC (AP) .................... ............ NUMBER EXAMINED: 10 NOT REMARKABLE: 10 17 17 PREPUTIAL GLAND (PG) ..................... ............ NUMBER EXAMINED: NOT REMARKABLE: LARGE CAVITY, ABDOM (PC) ....................... ............ NUMBER EXAMINED: NOT REMARKABLE: LARGE VESSELS AORTA (AO) ................................ ............ NUMBER EXAMINED: NOT REMARKABLE: 10 8 2 10 9 1 10 10 0 0 0 17 17 0 17 17 FOOT/FOOTPAD (FP) ........................ ............ NUMBER EXAMINED: NOT REMARKABLE: CRUSTED AREA(S) ABRASION(S)/ULCERATION(S) 10 9 1 0 17 16 0 1 368 TABLE 35 Incidence of Macroscopic Observations Recovery Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 13 TABLE INCLUDES: SEX=ALL;GROUP=6 ;WEEKS=ALL DEATH=U;SUBSET=ALL ORGAN AND KEYWORD(S) OR PHRASE -- N U M B E R - O F - A N I M A L S - A F F E C SEX: MALE FEMALE GROUP: -6- -6NUMBER: 10 17 T E D -- AUDITORY SEB GL (AS) ..................... ............ NUMBER EXAMINED: 10 NOT REMARKABLE: 10 17 17 CALVARIUM/SKULL (CA) ..................... ............ NUMBER EXAMINED: 10 NOT REMARKABLE: 10 17 17 LN, LUMBAR/ILIAC (LM) .................... ............ NUMBER EXAMINED: NOT REMARKABLE: CYST(S) LARGE URETER (UE) ............................... ............ NUMBER EXAMINED: NOT REMARKABLE: 10 8 0 2 10 10 17 16 1 1 17 17 JOINT, OTHER (JT) ........................ ............ NUMBER EXAMINED: 10 NOT REMARKABLE: 7 LARGE ADIPOSE TISSUE (AT) ...................... ............ NUMBER EXAMINED: NOT REMARKABLE: 3 10 10 17 17 0 17 17 369 TABLE 35 Incidence of Macroscopic Observations Recovery Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS TABLE INCLUDES: SEX=ALL;GROUP=6 ;WEEKS=ALL DEATH=U;SUBSET=ALL ORGAN AND KEYWORD(S) OR PHRASE -- N U M B E R - O F - A N I M A L S SEX: MALE FEMALE GROUP: -6- -6NUMBER: 10 17 VAS DEFERENS (VS) .......... NUMBER EXAMINED: 10 NOT REMARKABLE: 10 0 0 DIAPHRAGM (DP) .............. NUMBER EXAMINED: 10 NOT REMARKABLE: 10 17 17 LN, POPLITEAL (PP) ......... LARGE PERICARDIAL SAC (PD) ....... NUMBER EXAMINED: NOT REMARKABLE: NUMBER EXAMINED: NOT REMARKABLE: 10 9 1 10 10 17 17 0 17 17 ARTERY, OTHER (AU) ......... NUMBER EXAMINED: 10 NOT REMARKABLE: 10 17 17 AORTIC ARCH (AA) NUMBER EXAMINED: 10 NOT REMARKABLE: 10 17 17 LN, ILEOCECAL (IC) NUMBER EXAMINED: 10 NOT REMARKABLE: 10 17 17 Covance 6329-183 3M T-6295 PAGE: 14 A F F E C T E D -- 370 TABLE 35 Incidence of Macroscopic Observations Recovery Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS TABLE INCLUDES: SEX=ALL;GROUP=6 ;WEEKS=ALL DEATH=U;SUBSET=ALL ORGAN AND KEYWORD(S) OR PHRASE -- N U M B E R - O F - A N I M A L S SEX: MALE FEMALE GROUP: -6- -6NUMBER: 10 17 LN, TRACHEOBRON (TB) ....... . NUMBER EXAMINED: 10 NOT REMARKABLE: 10 17 17 MUSCLE, OTHER (MO) ......... . NUMBER EXAMINED: 10 NOT REMARKABLE: 10 17 17 PINNA (PN) .................. MASS(ES) BILE DUCT (BD) .............. . NUMBER EXAMINED: NOT REMARKABLE: . NUMBER EXAMINED: NOT REMARKABLE: 10 10 0 10 10 17 16 1 17 17 TRIGEMINAL NERVE (TG) ...... . NUMBER EXAMINED: 10 NOT REMARKABLE: 10 17 17 CLITORAL GLAND (CL) ........ ** END OF LIST ** . NUMBER EXAMINED: NOT REMARKABLE: 0 17 0 17 Covance 6329-183 3M T-6295 PAGE: 15 A F F E C T E D -- 371 TABLE 36 Incidence of Microscopic Observations Week 14 Interim Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS;; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 1 TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,5;WEEKS=ALL DEATH=2;FIND=ALL;SUBSET=ALL N U M BER - O F -ANI M A L S - A F F E < SEX: -MALE-- FEMALE-- GROUP: -1 - -2 - -3- -4- -5 - -1 - -2 - -3- -4 - -5- ORGAN AND FINDING DESCRIPTION NUMBER: 5 5 5 5 5 5 5 5 5 5 ** TOP OF LIST ** EYE (EY) ....................................... ...... NUMBER EXAMINED: 5 5 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 5 5 5 5 5 5 5 5 5 BRAIN (BR) ..................... ...................... NUMBER EXAMINED: 5 5 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 5 5 5 5 5 5 5 5 5 LIVER (LI) ............................................. NUMBER EXAMINED: 5 5 5 5 5 5 5 5 5 5 NOT REMARKABLE: 0 0 0 0 0 2 0 0 0 0 --INFILTRATE, LYMPHOHISTIOCYTIC --HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED --NECROSIS, INDIVIDUAL HEPATOCYTE --VACUOLATION, HEPATOCELLULAR, MIDZONAL / CENTRILOBULAR --VACUOLATION, HEPATOCELLULAR, PERIPORTAL --FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL --NECROSIS, COAGULATIVE 5555535555 00055 0001 4 22 01 2 1 22 1 3 00034 01 002 2 32 001 032 1 00000001 1 0 00000001 00 LIVER-OIL RED O (LI0) ................................. NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 KIDNEY (KD) ................................. .......... NUMBER EXAMINED: 5 5 5 5 5 5 5 5 5 5 NOT REMARKABLE: 0 1 1 2 1 2 5 2 3 5 --INFILTRATE, LYMPHOHISTIOCYTIC ** CONTINUED ON NEXT PAGE ** 422 32 3 031 0 372 TABLE 36 Incidence of Microscopic Observations Week 14 Interim Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS;; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 2 TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,5;WEEKS=ALL DEATH=2;FIND=ALL;SUBSET=ALL N U M BE R -O F -A N IMA L S -A F FE < SEX: -MALE-- FEMALE GROUP: -1 - -2 - -3- -4- -5- -1 - -2 - -3- -4- -5- ORGAN AND FINDING DESCRIPTION NUMBER: 5 5 5 5 5 5 5 5 5 5 ** FROM PREVIOUS PAGE ** KIDNEY (KD) .................................... ...... NUMBER EXAMINED: 5 5 5 5 5 5 5 5 5 5 NOT REMARKABLE: 0 1 1 2 1 2 5 2 3 5 --DILATATION, PELVIC --MINERALIZATION, PELVIC --REGENERATION, TUBULAR EPITHELIUM --MINERALIZATION, TUBULAR 02 1 1 2 001 1 0 00001 0001 0 1 01 31 1 0000 00000001 00 SPLEEN (SP) .................................... ...... NUMBER EXAMINED: 5 5 5 5 5 5 5 5 5 5 NOT REMARKABLE: 3 3 4 5 4 0 0 1 0 0 --PIGMENT, INCREASED 22 1 01 554 55 PANCREAS (PA) .................................. ...... NUMBER EXAMINED: 5 5 5 5 5 5 5 5 5 5 NOT REMARKABLE: 4 2 5 3 4 4 5 5 3 5 --ZYMOGEN GRANULES, DECREASED --INFILTRATE, LYMPHOHISTIOCYTIC --INFILTRATE, MACROPHAGE, PIGMENTED --HYPERPLASIA, ISLET CELLS 0002 1 0001 0 02 02 1 1 001 0 1 1 00000000 0001 000000 ADRENAL, CORTEX (AC) .......................... ...... NUMBER EXAMINED: 5 5 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 3 5 5 5 4 5 5 4 5 --VACUOLATION, FOCAL --UNILATERALLY EXAMINED 01 01 00000000 0001 001 0 373 TABLE 36 Incidence of Microscopic Observations Week 14 Interim Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS;; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 3 TABLE INCLUDES: SEX=ALL;GROUP=1,2,3/4 /5;WEEKS=ALL DEATH=2;FIND=ALL;SUBSET=ALL N U M BE R -O F -A N IMA L S -A F FE < SEX: -MALE-- FEMALE-- GROUP: -1 - -2 - -3- -4- -5- -1 - -2 - -3- -4 - -5- ORGAN AND FINDING DESCRIPTION NUMBER: 5 5 5 5 5 5 5 5 5 5 -------------------------------------------------- ------------------------- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ADRENAL, MEDULLA (MA) ......................... ...... NUMBER EXAMINED: 5 5 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 4 5 5 5 4 5 5 4 5 --UNILATERALLY EXAMINED 01 0001 001 0 LN, MESENTERIC (MS) ............................ ...... NUMBER EXAMINED: 5 5 5 4 5 5 5 5 5 5 NOT REMARKABLE: 5 5 5 4 4 5 5 5 5 5 --HEMORRHAGE 00001 00000 OVARY (OV) ..................................... ...... NUMBER EXAMINED: 0 0 0 0 0 5 5 5 5 5 NOT REMARKABLE: 0 0 0 0 0 5 5 5 5 5 TESTIS (TE) .................................... ...... NUMBER EXAMINED: 5 5 5 5 5 0 0 0 0 0 NOT REMARKABLE: 4 5 5 5 5 0 0 0 0 0 --ATROPHY/DEGENERATION 1 000000000 BONE, FEMUR (FE) ............................... ...... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 MARROW, FEMUR (FM) ............................. ...... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 374 TABLE 36 Incidence of Microscopic Observations Week 14 Interim Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 4 TABLE INCLUDES: SEX=ALL;GROUP=1,2,3/4 /5;WEEKS=ALL DEATH=2;FIND=ALL;SUBSET=ALL N U M BE R -O F -A N IMA L S -A F FE < SEX: -MALE-- FEMALE-- GROUP: -1 - -2 - -3- -4- -5- -1 - -2 - -3- -4 - -5- ORGAN AND FINDING DESCRIPTION -------------------------------------- -- NUMBER: 5 5 5 5 5 5 5 5 5 5 -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- MARROW, STERNUM (SE) ............... .................. NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 BONE, STERNUM (SB) ................. .................. NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 SPINAL CORD (SC) ................... .................. NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 THYMUS (TH) ........................ .................. NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 LUNG (LU) .......................... .................. NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 HEART (HT) ......................... .................. NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 MUSCLE, SKELETAL (SM) .............. .................. NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 375 TABLE 36 Incidence of Microscopic Observations Week 14 Interim Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 5 TABLE INCLUDES: SEX=ALL;GROUP=1,2,3/4 /5;WEEKS=ALL DEATH=2;FIND=ALL;SUBSET=ALL N U M BE R -O F -A N IMA L S -A F FE < SEX: -MALE-- FEMALE-- GROUP: -1 - -2 - -3- -4- -5- -1 - -2 - -3- -4 - -5- ORGAN AND FINDING DESCRIPTION -------------------------------------- -- NUMBER: 5 5 5 5 5 5 5 5 5 5 -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- NERVE, SCIATIC (SN) ................ .................. NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 TRACHEA (TR) ....................... .................. NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 ESOPHAGUS (ES) ..................... .................. NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 THYROID (TY) ....................... .................. NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 PARATHYROID (PT) ................... .................. NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 PITUITARY (PI) ..................... .................. NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 HARDERIAN GLAND (HG) ............... .................. NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 376 TABLE 36 Incidence of Microscopic Observations Week 14 Interim Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 6 TABLE INCLUDES: SEX=ALL;GROUP=1,2,3/4 /5;WEEKS=ALL DEATH=2;FIND=ALL;SUBSET=ALL N U M BE R -O F -A N IMA L S -A F FE < SEX: -MALE-- FEMALE-- GROUP: -1- -2- -3- -4- -5- -1- -2- -3- -4 - -5- ORGAN AND FINDING DESCRIPTION -------------------------------------- -- NUMBER: 5 5 5 5 5 5 5 5 5 5 -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- SALIV GL, MANDIB (SG) .............. .................. NUMBER EXAMINED: NOT REMARKABLE: 00 00 00 00 00 00 00 00 00 00 STOMACH, GL (ST) ................... .................. NUMBER EXAMINED: NOT REMARKABLE: 00 00 00 00 00 00 00 00 00 00 STOMACH, NONGL (SU) ................ .................. NUMBER EXAMINED: NOT REMARKABLE: 00 00 00 00 00 00 00 00 00 00 DUODENUM (DU) ...................... .................. NUMBER EXAMINED: NOT REMARKABLE: 00 00 00 00 00 00 00 00 00 00 JEJUNUM (JE) ....................... .................. NUMBER EXAMINED: NOT REMARKABLE: 00 00 00 00 00 00 00 00 00 00 ILEUM (IL) ......................... .................. NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 CECUM (CE) ......................... .................. NUMBER EXAMINED: NOT REMARKABLE: 00 00 00 00 00 00 00 00 00 00 377 TABLE 36 Incidence of Microscopic Observations Week 14 Interim Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 7 TABLE INCLUDES: SEX=ALL;GROUP=1,2,3/4 /5;WEEKS=ALL DEATH=2;FIND=ALL;SUBSET=ALL N U M BE R -O F -A N IMA L S -A F FE < SEX: -MALE-- FEMALE-- GROUP: -1 - -2 - -3- -4- -5- -1 - -2 - -3- -4 - -5- ORGAN AND FINDING DESCRIPTION -------------------------------------- -- NUMBER: 5 5 5 5 5 5 5 5 5 5 -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- COLON (CO) ......................... .................. NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 RECTUM (RE) ........................ .................. NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 SKIN (SK) .......................... .................. NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 MAMMARY, FEMALE (MF) ............... .................. NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 URINARY BLADDER (UB) ............... .................. NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 UTERUS (UT) ........................ .................. NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 CERVIX (CV) ........................ .................. NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 378 TABLE 36 Incidence of Microscopic Observations Week 14 Interim Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 8 TABLE INCLUDES: SEX=ALL;GROUP=1,2,3/4 /5;WEEKS=ALL DEATH=2;FIND=ALL;SUBSET=ALL N U M BE R -O F -A N IMA L S -A F FE < SEX: -MALE-- FEMALE-- GROUP: -1 - -2 - -3- -4- -5- -1 - -2 - -3- -4 - -5- ORGAN AND FINDING DESCRIPTION -------------------------------------- -- NUMBER: 5 5 5 5 5 5 5 5 5 5 -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- VAGINA (VA) ........................ .................. NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 PROSTATE (PR) ...................... .................. NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 SEMINAL VESICLES (SV) .............. .................. NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 EPIDIDYMIDES (EP) .................. .................. NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 HEMATO NEOPLASIA (HN) .............. .................. NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 CAUSE OF DEATH (CD) ................ .................. NUMBER EXAMINED: 5 5 5 5 5 5 5 5 5 5 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 --SCHEDULED SACRIFICE 5555555555 LN, MEDIASTINAL (ML) ............... .................. NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 379 TABLE 36 Incidence of Microscopic Observations Week 14 Interim Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS;; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 9 TABLE INCLUDES: SEX=ALL;GROUP=1,2,3/4 /5;WEEKS=ALL DEATH=2;FIND=ALL;SUBSET=ALL N U M BE R -O F -A N IMA L S -A F FE < SEX: -MALE-- FEMALE-- GROUP: -1 - -2 - -3- -4- -5- -1 - -2 - -3- -4 - -5- ORGAN AND FINDING DESCRIPTION NUMBER: 5 5 5 5 5 5 5 5 5 5 -------------------------------------------------- ------------------------- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- LN, AXILLARY (AX) .............................. ...... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 LN, INGUINAL (IN) .............................. ...... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 LN, OTHER (LN) ................................. ...... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 LN, RENAL (RL) ................................. ...... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 LN, MANDIBULAR (MN) ............................ ...... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 VERTEBRA/MARROW (VT) .......................... ...... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 MAMMARY, MALE (MM) ............................. ...... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 380 TABLE 36 Incidence of Microscopic Observations Week 14 Interim Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS;; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 10 TABLE INCLUDES: SEX=ALL;GROUP=1,2,3/4 /5;WEEKS=ALL DEATH=2;FIND=ALL;SUBSET=ALL N U M B E R - O F - A N I M A L S - A F F E < T E D -- SEX: MALE-- FEMALE-- GROUP: -1 - -2 - -3- -4- -5- -1 - -2 - -3- -4 - -5- ORGAN AND FINDING DESCRIPTION -- NUMBER: 5 5 5 5 5 5 5 5 5 5 ------------------------- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- COAGULATING GL (CG) ............................ ...... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 TAIL (TI) ...................................... ...... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 LN, LUMBAR/ILIAC (LM) ......................... ...... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 CAVITY, ABDOM (PC) ............................. ...... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 AUDITORY SEB GL (AS) .......................... ...... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 PREPUTIAL GLAND (PG) .......................... ...... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 FOOT/FOOTPAD (FP) .............................. ...... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 381 TABLE 36 Incidence of Microscopic Observations Week 14 Interim Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS;; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 11 TABLE INCLUDES: SEX=ALL;GROUP=1,2,3/4 /5;WEEKS=ALL DEATH=2;FIND=ALL;SUBSET=ALL N U M B E R - O F - A N I M A L S - A F F E < T E D -- SEX: MALE-- FEMALE-- GROUP: -1 - -2 - -3- -4- -5- -1 - -2 - -3- -4 - -5- ORGAN AND FINDING DESCRIPTION -- NUMBER: 5 5 5 5 5 5 5 5 5 5 ------------------------- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- AORTA (AO) ..................................... ...... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 CALVARIUM/SKULL (CA) .......................... ...... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 LN, ANT MES/PANC (AP) ......................... ...... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 HEAD, CORONAL (HC) ............................. ...... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 JOINT, OTHER (JT) .............................. ...... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 CLITORAL GLAND (CL) ............................ ...... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 URETER (UE) .................................... ...... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 382 TABLE 36 Incidence of Microscopic Observations Week 14 Interim Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 12 TABLE INCLUDES: SEX=ALL;GROUP=1,2,3/4 /5;WEEKS=ALL DEATH=2;FIND=ALL;SUBSET=ALL N U M B E R - O F - A N I M A L S - A F F E < T E D -- SEX: -MALE-- FEMALE-- GROUP: -1 - -2 - -3- -4- -5- -1 - -2 - -3- -4 - -5- ORGAN AND FINDING DESCRIPTION -------------------------------------- -- NUMBER: 5 5 5 5 5 5 5 5 5 5 -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ADIPOSE TISSUE (AT) ................ .................. NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 DIAPHRAGM (DP) ..................... .................. NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 CAVITY, THORACIC (TA) .............. .................. NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 LN, TRACHEOBRON (TB) ............... .................. NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 LN, POPLITEAL (PP) ................. .................. NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 MUSCLE, OTHER (MO) ................. .................. NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 PERICARDIAL SAC (PD) ............... .................. NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 383 TABLE 36 Incidence of Microscopic Observations Week 14 Interim Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 13 TABLE INCLUDES: SEX=ALL;GROUP=1,2,3/4 /5;WEEKS=ALL DEATH=2;FIND=ALL;SUBSET=ALL N U M B E R - O F - A N I M A L S - A F F E < T E D -- SEX: MALE-- FEMALE-- GROUP: -1 - -2 - -3- -4- -5- -1 - -2 - -3- -4 - -5- ORGAN AND FINDING DESCRIPTION -------------------------------------- -- NUMBER: 5 5 5 5 5 5 5 5 5 5 -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- PINNA (PN) ......................... .................. NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 ARTERY, OTHER (AU) ................. .................. NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 AORTIC ARCH (AA) ................... .................. NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 LN, ILEOCECAL (IC) ................. .................. NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 VAS DEFERENS (VS) .................. .................. NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 BILE DUCT (BD) ..................... .................. NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 TRIGEMINAL NERVE (TG) .............. .................. NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 ** END OF LIST ** 384 TABLE 37 Incidence of Microscopic Observations Week 53 Interim Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 1 TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=3;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---SEX: --MALE -- -FEMALEGROUP: -1 - -5- -1 - -5- ORGAN AND FINDING DESCRIPTION NUMBER: 10 9 10 10 -- ------------------------ -=- -=- -=- -=- ** TOP OF LIST ** EYE (EY) ........................................ ..... NUMBER EXAMINED: 10 9 10 10 NOT REMARKABLE: 10 9 10 10 BRAIN (BR) ...................................... ..... NUMBER EXAMINED: NOT REMARKABLE: --COMPRESSION, VENTRAL LIVER (LI) ...................................... ..... NUMBER EXAMINED: NOT REMARKABLE: --INFILTRATE, LYMPHOHISTIOCYTIC --HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED --PIGMENT, HEPATOCELLULAR, CENTRILOBULAR --NECROSIS, INDIVIDUAL HEPATOCYTE --VACUOLATION, HEPATOCELLULAR, MIDZONAL / CENTRILOBULAR --VACUOLATION, HEPATOCELLULAR, PERIPORTAL --FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL --FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC --HYPERPLASIA, BILE DUCT --HYPERTROPHY, HEPATOCELLULAR, PERIPORTAL --INFILTRATE, MACROPHAGE, PIGMENTED --VACUOLATION, HEPATOCELLULAR --HYPERTROPHY, HEPATOCELLULAR --CYST, BILIARY --INFLAMMATION, SUBACUTE 10 10 0 10 0 9 0 0 0 1 3 7 0 1 1 2 3 0 0 0 9 10 10 9 9 10 01 0 9 10 10 02 0 569 809 006 233 5 00 221 000 1 00 02 3 000 1 03 331 1 00 001 02 0 385 TABLE 37 Incidence of Microscopic Observations Week 53 Interim Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 2 TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=3;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---SEX: --MALE -- -FEMALEGROUP: -1 - -5- -1 - -5- ORGAN AND FINDING DESCRIPTION -- NUMBER: 10 9 10 10 -------------------------- -=- -=- -=- -=- LIVER-OIL RED O (LI0) ........................ ....... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 KIDNEY (KD) ................................... ....... NUMBER EXAMINED: NOT REMARKABLE: --NEPHROPATHY, CHRONIC PROGRESSIVE --INFILTRATE, LYMPHOHISTIOCYTIC --DILATATION, PELVIC --MINERALIZATION, PELVIC --REGENERATION, TUBULAR EPITHELIUM --MINERALIZATION, TUBULAR --PIGMENT, TUBULAR --HYALINE DROPLETS, TUBULAR EPITHELIUM --INFILTRATE, MACROPHAGE, PIGMENTED SPLEEN (SP) ................................... ....... NUMBER EXAMINED: NOT REMARKABLE: --PIGMENT, INCREASED --HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED --FIBROSIS, CAPSULAR PANCREAS (PA) ................................. ....... NUMBER EXAMINED: NOT REMARKABLE: --ZYMOGEN GRANULES, DECREASED ** CONTINUED ON NEXT PAGE ** 10 0 9 0 0 1 1 0 0 0 0 10 0 10 6 0 10 3 1 9 10 10 112 631 1 01 001 064 000 1 46 21 1 1 00 01 0 9 10 10 2 00 7 10 10 252 001 9 10 10 2 97 000 386 TABLE 37 Incidence of Microscopic Observations Week 53 Interim Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 3 TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=3;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---SEX: --MALE-- -FEMALEGROUP: -1 - -5- -1 - -5- ORGAN AND FINDING DESCRIPTION NUMBER: 10 9 10 10 -- --------------------------- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** PANCREAS (PA) ................................ ........ NUMBER EXAMINED: 10 9 10 10 NOT REMARKABLE: 3 2 9 7 --INFILTRATE, LYMPHOHISTIOCYTIC --INFILTRATE, MACROPHAGE, PIGMENTED --HYPERPLASIA, ISLET CELLS --ATROPHY, ACINAR --NECROSIS / INFLAMMATION, ADIPOSE TISSUE 1 01 2 57 01 2 000 1 1 01 001 0 ADRENAL, CORTEX (AC) ........................ ........ NUMBER EXAMINED: 10 NOT REMARKABLE: 2 9 10 10 21 0 --UNILATERALLY EXAMINED --INFILTRATE, MACROPHAGE, PIGMENTED --HEMORRHAGE --HYPERTROPHY, CORTICAL CELL --VACUOLATION, DIFFUSE --DEGENERATION, CYSTIC / HEMORRHAGIC --HYPERPLASIA, CORTICAL 001 0 6458 001 0 2 3 03 01 00 004 1 0001 ADRENAL, MEDULLA (MA) ....................... ........ NUMBER EXAMINED: 10 NOT REMARKABLE: 10 9 10 10 999 --UNILATERALLY EXAMINED 001 1 387 TABLE 37 Incidence of Microscopic Observations Week 53 Interim Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 4 TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=3;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---SEX: --MALE -- -FEMALEGROUP: -1 - -5- -1 - -5- ORGAN AND FINDING DESCRIPTION ------------------------------------ -- NUMBER: 10 9 10 10 -=- -=- -=- -=- LN, MESENTERIC (MS) .............. .................... NUMBER EXAMINED: 10 NOT REMARKABLE: 10 9 10 10 8 10 10 --HEMORRHAGE 01 00 OVARY (OV) ....................... .................... NUMBER EXAMINED: NOT REMARKABLE: 0 0 0 10 10 0 9 10 --CYST 001 0 TESTIS (TE) ...................... .................... NUMBER EXAMINED: 10 9 0 0 NOT REMARKABLE: 8 9 0 0 --ATROPHY/DEGENERATION --GRANULOMA, SPERM 2 000 1 000 BONE, FEMUR (FE) ................. .................... NUMBER EXAMINED: 10 NOT REMARKABLE: 10 9 10 10 9 10 10 MARROW, FEMUR (FM) ............... .................... NUMBER EXAMINED: NOT REMARKABLE: --HYPOCELLULAR, MARROW --HYPERPLASIA, MYELOID, MARROW 10 5 5 0 9 10 10 6 10 8 3 01 001 388 TABLE 37 Incidence of Microscopic Observations Week 53 Interim Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 5 TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=3;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---SEX: --MALE -- -FEMALEGROUP: -1 - -5- -1 - -5- ORGAN AND FINDING DESCRIPTION --------------------------------------------------------- -- NUMBER: 10 9 10 10 -=- -=- -=- -=- MARROW, STERNUM (SE) ................................. . NUMBER EXAMINED: 10 NOT REMARKABLE: 9 9 10 10 9 10 9 --HYPERPLASIA, MYELOID, MARROW --HYPOCELLULAR, MARROW 0001 1 000 BONE, STERNUM (SB) ................................... . NUMBER EXAMINED: 10 NOT REMARKABLE: 10 9 10 10 9 10 10 SPINAL CORD (SC) ..................................... . NUMBER EXAMINED: 10 NOT REMARKABLE: 10 9 10 10 9 10 10 THYMUS (TH) .......................................... . NUMBER EXAMINED: NOT REMARKABLE: --HEMORRHAGE --DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY) --M-THYMIC CARCINOMA --CYST LUNG (LU) ............................................. . NUMBER EXAMINED: NOT REMARKABLE: --CONGESTION --MINERALIZATION, VASCULAR ** CONTINUED ON NEXT PAGE ** 7 5 2 2 0 0 10 6 1 1 8 10 27 9 6 322 31 0 1 00 001 9 10 10 554 000 31 0 389 TABLE 37 Incidence of Microscopic Observations Week 53 Interim Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 6 TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=3;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---SEX: --MALE -- -FEMALEGROUP: -1- -5- -1- -5- ORGAN AND FINDING DESCRIPTION NUMBER: 10 9 10 10 ----------------------------------------- ---------------------------------- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** LUNG (LU) ............................. ................ NUMBER EXAMINED: NOT REMARKABLE: 106 9 10 10 554 --HEMORRHAGE --INFILTRATE, MACROPHAGE, ALVEOLAR --INFILTRATE, LYMPHOHISTIOCYTIC 1121 10 11 2 3 42 10HEART (HT) ............................ ................ NUMBER EXAMINED: NOT REMARKABLE: 7 9 10 10 5 8 10 --CARDIOMYOPATHY --HEMORRHAGE 30 41 20 00 10MUSCLE, SKELETAL (SM) ................ ................ NUMBER EXAMINED: NOT REMARKABLE: 10 9 10 10 9 10 10 10NERVE, SCIATIC (SN) .................. ................ NUMBER EXAMINED: 10NOT REMARKABLE: 9 9 1100 1100 10TRACHEA (TR) ......................... ................ NUMBER EXAMINED: 10NOT REMARKABLE: 9 9 1100 1100 10 10ESOPHAGUS (ES) ....................... ................ NUMBER EXAMINED: 10 10NOT REMARKABLE: 99 99 390 TABLE 37 Incidence of Microscopic Observations Week 53 Interim Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 7 TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=3;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---- SEX: --MALE -- -FEMALE- GROUP: -1 - -5- -1 - -5- NUMBER: 10 9 10 10 THYROID (TY) ...................... ................... NUMBER EXAMINED: NOT REMARKABLE: --HYPERPLASIA, C-CELL --M-CARCINOMA, FOLLICULAR CELL PARATHYROID (PT) .................. ................... NUMBER EXAMINED: NOT REMARKABLE: 10 8 1 1 10 10 9 10 10 9 10 10 000 000 9 10 10 9 10 10 PITUITARY (PI) .................... ................... NUMBER EXAMINED: NOT REMARKABLE: --B-ADENOMA --HYPERPLASIA --ANGIECTASIS HARDERIAN GLAND (HG) .............. ................... NUMBER EXAMINED: NOT REMARKABLE: --INFILTRATE, LYMPHOHISTIOCYTIC SALIV GL, MANDIB (SG) ............. ................... NUMBER EXAMINED: NOT REMARKABLE: 10 4 4 2 1 10 10 0 10 10 9 10 10 855 1 42 114 03 3 9 10 10 899 111 9 10 10 9 10 10 391 TABLE 37 Incidence of Microscopic Observations Week 53 Interim Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 8 TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=3;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---SEX: --MALE -- -FEMALEGROUP: -1 - -5- -1 - -5- ORGAN AND FINDING DESCRIPTION ----------------------------------- -- NUMBER: 10 9 10 10 -=- -=- -=- -=- STOMACH, GL (ST) ................ ..................... NUMBER EXAMINED: 10 NOT REMARKABLE: 9 9 10 10 7 8 10 --EDEMA --INFLAMMATION, ACUTE --CONGESTION --EROSION 001 0 1 02 0 01 00 01 00 STOMACH, NONGL (SU) ............. ..................... NUMBER EXAMINED: 10 NOT REMARKABLE: 9 9 10 10 9 10 10 --ACANTHOSIS / HYPERKERATOSIS 1 000 DUODENUM (DU) ................... ..................... NUMBER EXAMINED: 10 NOT REMARKABLE: 10 9 10 10 9 10 10 JEJUNUM (JE) .................... ..................... NUMBER EXAMINED: 10 NOT REMARKABLE: 10 9 10 10 9 10 10 ILEUM (IL) ...................... ..................... NUMBER EXAMINED: 10 NOT REMARKABLE: 10 9 10 10 9 10 10 CECUM (CE) ...................... ..................... NUMBER EXAMINED: 10 NOT REMARKABLE: 10 9 10 10 9 10 10 392 TABLE 37 Incidence of Microscopic Observations Week 53 Interim Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 9 TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=3;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---- SEX: --MALE -- -FEMALE- GROUP: -1 - -5- -1 - -5- NUMBER: 10 9 10 10 COLON (CO) .............................. .............. NUMBER EXAMINED: 10 NOT REMARKABLE: 10 9 10 10 9 10 10 RECTUM (RE) ............................. .............. NUMBER EXAMINED: NOT REMARKABLE: --PARASITISM SKIN (SK) ............................... .............. NUMBER EXAMINED: NOT REMARKABLE: --ACANTHOSIS/HYPERKERATOSIS --INFLAMMATION, CHRONIC-ACTIVE --ULCERATION --HEMORRHAGE --DEGENERATION, MUSCLE, SUBCUTANEOUS MAMMARY, FEMALE (MF) ................... .............. NUMBER EXAMINED: NOT REMARKABLE: --CYST / GALACTOCELE --HYPERPLASIA, GLANDULAR --SECRETORY MATERIAL --M-FIBROSARCOMA 10 10 0 10 10 0 0 0 0 0 0 0 0 0 0 0 9 10 10 7 10 10 2 00 9 10 10 9 8 10 02 0 02 0 02 0 01 0 01 0 0 10 10 032 032 04 4 06 5 01 0 393 TABLE 37 Incidence of Microscopic Observations Week 53 Interim Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 10 TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=3;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C SEX: --MALE -- -FEMALEGROUP: -1 - -5- -1 - -5- ORGAN AND FINDING DESCRIPTION -- NUMBER: 10 9 10 10 ----------------------------------- -=- -=- -=- -=- URINARY BLADDER (UB) ................ ................. NUMBER EXAMINED: 10 NOT REMARKABLE: 7 9 10 7 10 9 8 --INFILTRATE, LYMPHOHISTIOCYTIC --PROTEINACEOUS MATERIAL, LUMINAL 3 001 02 00 UTERUS (UT) ......................... ................. NUMBER EXAMINED: NOT REMARKABLE: 0 0 0 10 10 0 10 7 --DILATATION 0003 CERVIX (CV) ......................... ................. NUMBER EXAMINED: NOT REMARKABLE: 0 0 0 10 10 0 9 10 --CYST, SQUAMOUS 001 0 VAGINA (VA) ......................... ................. NUMBER EXAMINED: NOT REMARKABLE: 0 0 0 10 0 10 9 9 T E D -- PROSTATE (PR) ....................... ................. NUMBER EXAMINED: 10 9 0 0 NOT REMARKABLE: 4 6 0 0 --INFLAMMATION, CHRONIC-ACTIVE --INFILTRATE, LYMPHOHISTIOCYTIC 5 3 00 1 000 394 TABLE 37 Incidence of Microscopic Observations Week 53 Interim Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 11 TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=3;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C SEX: --MALE -- -FEMALEGROUP: -1 - -5- -1 - -5- ORGAN AND FINDING DESCRIPTION ------------------------------------- -- NUMBER: 10 9 10 10 -=- -=- -=- -=- SEMINAL VESICLES (SV) ............. ................... NUMBER EXAMINED: 10 9 0 0 NOT REMARKABLE: 10 9 0 0 T E D -- EPIDIDYMIDES (EP) ................. ................... NUMBER EXAMINED: NOT REMARKABLE: --IMMATURE SPERM FORMS --INFILTRATE, LYMPHOHISTIOCYTIC HEMATO NEOPLASIA (HN) ............. ................... NUMBER EXAMINED: NOT REMARKABLE: 10 9 0 1 10 10 900 7 00 1 00 1 00 9 10 10 9 10 10 CAUSE OF DEATH (CD) ............... ................... NUMBER EXAMINED: NOT REMARKABLE: --SCHEDULED SACRIFICE LN, MEDIASTINAL (ML) .............. ................... NUMBER EXAMINED: NOT REMARKABLE: 10 0 10 0 0 9 10 10 000 9 10 10 000 000 LN, AXILLARY (AX) ................. ................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 395 TABLE 37 Incidence of Microscopic Observations Week 53 Interim Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 12 TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=3;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C SEX: --MALE -- -FEMALEGROUP: -1 - -5- -1 - -5- ORGAN AND FINDING DESCRIPTION -- NUMBER: 10 9 10 10 --------------------------------- -=- -=- -=- -=- LN, INGUINAL (IN) ..................... ............... NUMBER EXAMINED: 0 0 1 0 NOT REMARKABLE: 0 0 0 0 --INFILTRATE, MACROPHAGE, PIGMENTED --HEMORRHAGE 001 001 0 0 LN, OTHER (LN) ........................ ............... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 T E D -- LN, RENAL (RL) ........................ ............... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 LN, MANDIBULAR (MN) ................... ............... NUMBER EXAMINED: 1 02 1 NOT REMARKABLE: 0 0 0 0 --HYPERPLASIA, LYMPHOPLASMACYTIC --HEMORRHAGE 002 0 1 02 1 VERTEBRA/MARROW (VT) .................. ............... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 MAMMARY, MALE (MM) .................... ............... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 396 TABLE 37 Incidence of Microscopic Observations Week 53 Interim Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 13 TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=3;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R - O F - A N I M A L S - A F F E C SEX: --MALE -- -FEMALE- GROUP: -1 - -5- -1 - -5- NUMBER: 10 9 10 10 T E D -- COAGULATING GL (CG) ......... ........................ NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 TAIL (TI) .................... ........................ NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 LN, LUMBAR/ILIAC (LM) ....... ........................ NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 CAVITY, ABDOM (PC) .......... ........................ NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 AUDITORY SEB GL (AS) ........ ........................ NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 PREPUTIAL GLAND (PG) ........ ........................ NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 FOOT/FOOTPAD (FP) ............ ........................ NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 397 TABLE 37 Incidence of Microscopic Observations Week 53 Interim Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 14 TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=3;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R - O F - A N I M A L S - A F F E C SEX: --MALE -- -FEMALE- GROUP: -1 - -5- -1 - -5- NUMBER: 10 9 10 10 T E D -- AORTA (AO) ................... ........................ NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 CALVARIUM/SKULL (CA) ........ ........................ NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 LN, ANT MES/PANC (AP) ....... ........................ NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 HEAD, CORONAL (HC) .......... ........................ NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 JOINT, OTHER (JT) ............ ........................ NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 CLITORAL GLAND (CL) ......... ........................ NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 URETER (UE) .................. ........................ NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 398 TABLE 37 Incidence of Microscopic Observations Week 53 Interim Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 15 TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=3;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C SEX: --MALE -- -FEMALEGROUP: -1 - -5- -1 - -5- ORGAN AND FINDING DESCRIPTION -------------------------------- -- NUMBER: 10 9 10 10 -=- -=- -=- -=- ADIPOSE TISSUE (AT) ......... ........................ NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 T E D -- DIAPHRAGM (DP) ............... ........................ NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 CAVITY, THORACIC (TA) ....... ........................ NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 LN, TRACHEOBRON (TB) ........ ........................ NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 LN, POPLITEAL (PP) .......... ........................ NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 MUSCLE, OTHER (MO) .......... ........................ NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 PERICARDIAL SAC (PD) ........ ........................ NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 399 TABLE 37 Incidence of Microscopic Observations Week 53 Interim Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 16 TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=3;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C SEX: --MALE -- -FEMALEGROUP: -1 - -5- -1 - -5- ORGAN AND FINDING DESCRIPTION -------------------------------- -- NUMBER: 10 9 10 10 -=- -=- -=- -=- PINNA (PN) ................... ........................ NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 T E D -- ARTERY, OTHER (AU) .......... ........................ NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 AORTIC ARCH (AA) ............. ........................ NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 LN, ILEOCECAL (IC) .......... ........................ NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 VAS DEFERENS (VS) ............ ........................ NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 BILE DUCT (BD) ............... ........................ NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 TRIGEMINAL NERVE (TG) ....... ........................ NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 ** END OF LIST ** 400 TABLE 38 Incidence of Microscopic Observations Terminal Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS Covance 6329-183 3M T-6295 PAGE: 1 TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,5;WEEKS=ALL DEATH=T;FIND=ALL;SUBSET=ALL -- N U M BE R -O F -A N IMA L S -A F FE ' SEX: -MALE- FEMALE GROUP: -1 - -2 - -3- -4- -5- -1 - -2 - -3- -4- -5- ORGAN AND FINDING DESCRIPTION NUMBER: 11 10 17 25 22 24 15 9 15 25 -- -- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ** TOP OF LIST ** EYE (EY) ............................................... NUMBER EXAMINED: 11 0 0 0 22 24 0 0 0 25 NOT REMARKABLE: 9 0 0 0 20 22 0 0 0 23 --DYSTROPHY, CORNEAL IN EARLY STAGES, CONSISTS PRIMARILY OF FOCAL MINERALIZATION --DEGENERATION, RETINAL 2 0002 1 000001 0002 0000 BRAIN (BR) ............................................. NUMBER EXAMINED: 11 0 7 5 22 24 8 5 8 25 NOT REMARKABLE: 9 0 3 0 14 11 1 2 1 14 --DILATATION, VENTRICULAR --COMPRESSION, VENTRAL --HEMORRHAGE --MINERALIZATION --X-HEMATOPOIETIC NEOPLASIA --I-CARCINOMA (SEE "HEMATO NEOPLASIA" FOR TYPE) 00001 22 1 01 2 0 4 5 6 10 7 3 5 11 000001 0000 001 01 1 001 1 00001 00000 000001 001 0 LIVER (LI) ............................................. NUMBER EXAMINED: 11 10 17 25 22 24 15 NOT REMARKABLE: 0 0 0 0 0 0 0 9 15 25 000 --INFILTRATE, LYMPHOHISTIOCYTIC --HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED --GRANULAR CYTOPLASM, EOSINOPHILIC, CENTRILOBULAR --PIGMENT, HEPATOCELLULAR, CENTRILOBULAR --NECROSIS, INDIVIDUAL HEPATOCYTE --VACUOLATION, HEPATOCELLULAR, MIDZONAL / CENTRILOBULAR --VACUOLATION, HEPATOCELLULAR, PERIPORTAL 11 10 13 23 20 17 14 0 1 3 13 18 1 0 0000900 00003 00 1 1 4271 2 005 7 8 00 0054 375 6 12 23 1 7 20 0 6 20 0 1 17 224 002 6 4 11 401 TABLE 38 Incidence of Microscopic Observations Terminal Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS Covance 6329-183 3M T-6295 PAGE: 2 TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,5;WEEKS=ALL DEATH=T;FIND=ALL;SUBSET=ALL -- N U M BER - O F - ANI M A LS- A F F E ' SEX: -MALE- FEMALE GROUP: -1 - -2 - -3- -4- -5- -1 - -2 - -3- -4- -5- ORGAN AND FINDING DESCRIPTION NUMBER: 11 10 17 25 22 24 15 9 15 25 -- ----------------- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** LIVER (LI) ............................................. NUMBER EXAMINED: 11 10 17 25 22 24 15 NOT REMARKABLE: 0 0 0 0 0 0 0 9 15 25 000 --FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL --FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC --DEGENERATION, CYSTIC --NECROSIS, COAGULATIVE --CONGESTION --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) --HEMATOPOIESIS, EXTRAMEDULLARY --HYPERPLASIA, BILE DUCT --FIBROSIS, PERIBILIARY --HYPERTROPHY, HEPATOCELLULAR, PERIPORTAL --INFILTRATE, MACROPHAGE, PIGMENTED --VACUOLATION, HEPATOCELLULAR --DEGENERATION / NECROSIS, CENTRILOBULAR --CYST, BILIARY --ANGIECTASIS --B-ADENOMA, HEPATOCELLULAR --LIPIDOSIS, SUBCAPSULAR (TENSION LIPIDOSIS) --N-CARCINOMA 7 7 10 20 17 18 6 6 8 15 9 3 12 16 10 11 11 5 10 15 3 4 8 13 14 0 0 1 0 1 01 001 001 1 2 000001 0000 00001 00000 1 02 1 32 2 4 4 6 8 5 8 13 15 14 10 3 7 14 4244877235 002 0091 1 2 2 0 0 0 0 1 1 0 0 1 11 2 1 00000001 0001 000000 000001 0002 001 01 4 1 2 1 0 01 1 06 01 01 4 002 1 01 002 0 0001 000000 LIVER-OIL RED O (LI0) ................................. NUMBER EXAMINED: 5 0 0 0 5 5 0 0 0 5 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 --VACUOLES, OIL RED O-POSITIVE, HEPATOCELLULAR 5 00055 0005 402 TABLE 38 Incidence of Microscopic Observations Terminal Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS Covance 6329-183 3M T-6295 PAGE: 3 TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,5;WEEKS=ALL DEATH=T;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- SEX: GROUP: NUMBER: -- KIDNEY (KD) ............................................ NUMBER EXAMINED: NOT REMARKABLE: --NEPHROPATHY, CHRONIC PROGRESSIVE --DILATATION, PELVIC --MINERALIZATION, PELVIC --MINERALIZATION, TUBULAR --VACUOLATION, TUBULAR EPITHELIUM --HYALINE DROPLETS, TUBULAR EPITHELIUM --HYPERPLASIA, TRANSITIONAL CELL --INFLAMMATION, SUPPURATIVE, PELVIC --INFLAMMATION, CHRONIC-ACTIVE, PELVIC --ECTASIA, TUBULAR --CYST --INFLAMMATION, CHRONIC-ACTIVE --M-CARCINOMA, TUBULAR CELL --THROMBUS --B-LIPOMA --M-RENAL MESENCHYMAL TUMOR --DEGENERATION TUBULAR --INFARCT SPLEEN (SP) ............................................ NUMBER EXAMINED: NOT REMARKABLE: --PIGMENT, INCREASED --HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) -- N U M BER - O F - ANI -MALE- -1- -2- -3- -4- -5- -1- 11 10 17 25 22 24 -=- -=- -=- -=- -=- -=- 111 100 171 250 220 240 10211000000 10100000000 160 31100000 240 5100 3110 22211000000 212 18 310 3100 00000000 20000000 10000000 22000000 11100000 11111100 111 00 00 00 226 240 480 000 000 000 6 131 21150 M A LS- A F F E ' FEMALE -2- -3- -4- -5- 15 9 15 25 -=- -=- -=- -=- 152 19 152 250 111122100000 8611100000 110211000000 221 18 400 3000 00000000 00000000 00000000 00000000 00 00 10 251 000 000 100 18 170 403 TABLE 38 Incidence of Microscopic Observations Terminal Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS Covance 6329-183 3M T-6295 PAGE: 4 TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,5;WEEKS=ALL DEATH=T;FIND=ALL;SUBSET=ALL -- N U M BER - O F - ANI M A LS- A F F E SEX: -MALE- FEMALE GROUP: -1 - -2 - -3- -4- -5- -1 - -2 - -3- -4- -5- ORGAN AND FINDING DESCRIPTION NUMBER: 11 10 17 25 22 24 15 9 15 25 ** FROM PREVIOUS PAGE ** SPLEEN (SP) ............................................ NUMBER EXAMINED: 11 NOT REMARKABLE: 1 0 0 0 22 24 0 0 1 25 0006 00001 --HYPERPLASIA, LYMPHORETICULAR 000000001 0 PANCREAS (PA) ......................................... NUMBER EXAMINED: 11 10 17 25 22 24 15 NOT REMARKABLE: 3 1 1 3 3 14 10 9 15 25 6 5 13 --ZYMOGEN GRANULES, DECREASED --INFILTRATE, LYMPHOHISTIOCYTIC --INFILTRATE, MACROPHAGE, PIGMENTED --HYPERPLASIA, ISLET CELLS --ATROPHY, ACINAR --HYPERTROPHY / BASOPHILIA, ACINAR CELL --M-CARCINOMA, ISLET CELL --B-ADENOMA, ISLET CELL --INFILTRATION, FAT, INTERSTITIAL --INFLAMMATION, VASCULAR --FIBROSIS --ECTOPIC HEPATOCYTES --B-FIBROMA --INFLAMMATION, CHRONIC-ACTIVE 1 1 3471 21 1 4 001 2 000000 21 431 1 0000 01 1 01 00000 2 3 34 4 02 1 1 2 1 1 002 1 1 01 0 001 54 00000 01 2 2 2 2 1 1 02 6 6 13 10 6 6 2 2 7 5 1 0031 00000 0000000001 0001 000000 0000000001 00000001 01 ADRENAL, CORTEX (AC) .................................. NUMBER EXAMINED: 11 2 0 1 22 24 5 1 5 25 NOT REMARKABLE: 1 0002 00002 --VACUOLATION, FOCAL --ANGIECTASIS 2 00084 0001 1 1 0 0 0 14 1 0 1 6 404 TABLE 38 Incidence of Microscopic Observations Terminal Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS Covance 6329-183 3M T-6295 PAGE: 5 TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,5;WEEKS=ALL DEATH=T;FIND=ALL;SUBSET=ALL -- N U M BER - O F - ANI M A LS- A F F E SEX: MALE-- FEMALE GROUP: -1 - -2 - -3- -4- -5- -1 - -2 - -3- -4- -5- ORGAN AND FINDING DESCRIPTION NUMBER: 11 10 17 25 22 24 15 9 15 25 ** FROM PREVIOUS PAGE ** ADRENAL, CORTEX (AC) ...................... NUMBER EXAMINED: 11 2 0 1 22 24 5 1 5 25 NOT REMARKABLE: 1 0002 00002 --HEMATOPOIESIS, EXTRAMEDULLARY --HYPERTROPHY, CORTICAL CELL --DEGENERATION, CYSTIC / HEMORRHAGIC --HYPERPLASIA, CORTICAL --PIGMENT --B-ADENOMA --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" --CORTICAL TISSUE, EXTRACAPSULAR FOR TYPE) 000001 01 00 3 0 0 0 8 16 4 1 3 16 1 0 0 0 1 11 3 1 3 13 2 00034 001 1 9 0 0 1 13 22 5 1 4 23 1 0001 0001 1 00001 00000 01 00000000 ADRENAL, MEDULLA (MA) ..................... NUMBER EXAMINED: 11 2 0 1 22 24 5 1 5 25 NOT REMARKABLE: 8 1 0 0 15 18 3 0 2 19 --UNILATERALLY EXAMINED --HYPERPLASIA, MEDULLARY --B-PHEOCHROMOCYTOMA --M-MALIGNANT PHEOCHROMOCYTOMA 1 0001 31 1 2 3 2 00031 0001 1 1 01 2 31 01 3 00001 00000 LN, MESENTERIC (MS) ....................... NUMBER EXAMINED: 11 0 0 1 22 24 3 0 0 25 NOT REMARKABLE: 10 0 0 0 20 23 1 0 0 23 --HEMORRHAGE --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" --HYPERPLASIA, LYMPHOPLASMACYTIC --INFILTRATE, MACROPHAGE, PIGMENTED --INFLAMMATION, VASCULAR FOR TYPE) 1 00001 1 001 00001 00000 0001 1 00000 000001 1 000 0001 000000 405 TABLE 38 Incidence of Microscopic Observations Terminal Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS Covance 6329-183 3M T-6295 PAGE: 6 TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,5;WEEKS=ALL DEATH=T;FIND=ALL;SUBSET=ALL -- N U M BER - O F - ANI M A LS- A F F E SEX: -MALE- FEMALE GROUP: -1 - -2 - -3- -4- -5- -1 - -2 - -3- -4- -5- ORGAN AND FINDING DESCRIPTION NUMBER: 11 10 17 25 22 24 15 9 15 25 ** FROM PREVIOUS PAGE ** LN, MESENTERIC (MS) ............................... .... NUMBER EXAMINED: 11 0 0 1 22 24 3 0 0 25 NOT REMARKABLE: 10 0 0 0 20 23 1 0 0 23 --LYMPHANGIECTASIS 0000000001 OVARY (OV) ........................................ ... NUMBER EXAMINED: 0 0 0 0 0 24 2 2 6 25 NOT REMARKABLE: 0 0 0 0 0 15 01 0 12 --CYST --HYPERPLASIA, GRANULOSA CELL --UNILATERALLY EXAMINED 0 0 0 0 0 8 2 0 6 10 000002 0005 00000001 02 TESTIS (TE) ....................................... ... NUMBER EXAMINED: 11 10 17 25 22 0 0 0 0 0 NOT REMARKABLE: 9 4 10 17 15 0 0 0 0 0 --ATROPHY/DEGENERATION --INFLAMMATION, VASCULAR --MINERALIZATION, VASCULAR --HYPERPLASIA, INTERSTITIAL CELL --B-INTERSTITIAL CELL TUMOR --INFILTRATE, LYMPHOHISTIOCYTIC (LEYDIG CELL) 2 4 54 5 00000 02 1 3 000000 01 01 1 00000 001 01 00000 01 22 000000 001 01 00000 BONE, FEMUR (FE) .................................. ... NUMBER EXAMINED: 11 NOT REMARKABLE: 11 0 0 0 22 24 0 0 0 22 24 0 0 0 25 0 0 0 25 406 TABLE 38 Incidence of Microscopic Observations Terminal Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS Covance 6329-183 3M T-6295 PAGE: 7 TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,5;WEEKS=ALL DEATH=T;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M BER - O F - ANI M A LS- A F F E SEX: -MALE-- FEMALE GROUP: -1 - -2 - -3- -4- -5- -1 - -2 - -3- -4- -5- NUMBER: 11 10 17 25 22 24 15 9 15 25 MARROW, FEMUR (FM) ..................... .............. NUMBER EXAMINED: 11 NOT REMARKABLE: 11 0 0 0 22 24 0 0 0 21 14 0 0 0 25 0 0 0 18 --HYPOCELLULAR, MARROW --HYPERPLASIA, MYELOID, MARROW --HYPERPLASIA, ERYTHROID, MARROW 00001 5 0002 000005 0004 0000000001 MARROW, STERNUM (SE) ................... .............. NUMBER EXAMINED: 11 NOT REMARKABLE: 11 0 0 0 22 24 0 0 0 22 19 0 0 0 25 0 0 0 21 --HYPERPLASIA, MYELOID, MARROW --HYPERPLASIA, ERYTHROID, MARROW 000005 0003 0000000001 BONE, STERNUM (SB) ..................... .............. NUMBER EXAMINED: 11 NOT REMARKABLE: 11 0 0 0 22 24 0 0 0 21 23 0 0 0 25 0 0 0 24 --X-HEMATOPOIETIC NEOPLASIA (SEE HN) --HYPERPLASIA, CARTILAGINOUS 00001 00000 000001 0001 SPINAL CORD (SC) ....................... .............. NUMBER EXAMINED: 11 NOT REMARKABLE: 11 0 0 0 22 24 0 0 0 22 23 0 0 0 25 0 0 0 25 --DEGENERATION, AXONAL --INFILTRATE, LYMPHOHISTIOCYTIC 000001 000001 0000 0000 407 TABLE 38 Incidence of Microscopic Observations Terminal Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS Covance 6329-183 3M T-6295 PAGE: 8 TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,5;WEEKS=ALL DEATH=T;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M BER - O F - ANI M A LS- A F F E SEX: -MALE- FEMALE GROUP: -1 - -2 - -3- -4- -5- -1 - -2 - -3- -4- -5- NUMBER: 11 10 17 25 22 24 15 9 15 25 THYMUS (TH) ................................ NUMBER EXAMINED: 9 0 0 1 19 22 0 NOT REMARKABLE: 2 0 0 0 1 4 0 --DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY) --HYPERPLASIA, LYMPHORETICULAR 7 0 0 1 18 17 0 000001 0 LUNG (LU) .................................. NUMBER EXAMINED: 11 10 17 24 22 24 15 NOT REMARKABLE: 2 7 10 11 10 14 8 --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" --MINERALIZATION, VASCULAR --N-CARCINOMA --HEMORRHAGE --INFILTRATE, MACROPHAGE, ALVEOLAR --INFILTRATE, LYMPHOHISTIOCYTIC --HYPERPLASIA, TYPE II PNEUMOCYTE --N-SARCOMA --FIBROSIS, PLEURAL/SUBPLEURAL FOR TYPE) 00001 00 6 1 5 10 7 3 1 0001 01 1 00001 00 421 1 664 2 02 01 2 1 1 02 02 01 0001 000 0000000 HEART (HT) ................................. NUMBER EXAMINED: 11 0 0 0 22 24 0 NOT REMARKABLE: 0 0 0 0 1 12 0 --CARDIOMYOPATHY --MINERALIZATION, VASCULAR --M-ENDOCARDIAL SCHWANNOMA 11 0 0 0 21 12 0 00001 00 0000000 0 0 25 005 0 0 20 000 9 15 25 488 000 1 57 001 000 5 4 12 02 5 01 1 000 001 0 0 25 0 0 11 0 0 13 000 001 408 TABLE 38 Incidence of Microscopic Observations Terminal Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS Covance 6329-183 3M T-6295 PAGE: 9 TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,5;WEEKS=ALL DEATH=T;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION N U M BER - O F - ANI M A LS- A F F E < SEX: MALE-- FEMALE GROUP: -1 - -2 - -3- -4- -5- -1 - -2 - -3- -4- -5- NUMBER: 11 10 17 25 22 24 15 9 15 25 MUSCLE, SKELETAL (SM) .............. NUMBER EXAMINED: 11 NOT REMARKABLE: 10 0 0 0 22 24 0 0 0 20 24 0 0 --DEGENERATION --ATROPHY --M-SARCOMA, UNDIFFERENTIATED 00001 00 00001 00 1 000000 NERVE, SCIATIC (SN) ................ NUMBER EXAMINED: 11 NOT REMARKABLE: 11 0 0 0 22 24 0 0 0 21 24 0 0 --DEGENERATION 00001 00 TRACHEA (TR) ....................... NUMBER EXAMINED: 11 NOT REMARKABLE: 11 0 0 0 22 24 0 0 0 21 24 0 0 --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 00001 00 ESOPHAGUS (ES) ..................... NUMBER EXAMINED: 11 NOT REMARKABLE: 11 0 0 0 22 24 0 0 0 21 24 0 0 --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 00001 00 THYROID (TY) ....................... NUMBER EXAMINED: 11 10 17 24 22 24 15 NOT REMARKABLE: 4 3 5 5 6 10 4 --HYPERPLASIA, C-CELL --M-CARCINOMA, FOLLICULAR CELL --DILATATION / CYST, FOLLICULAR 3 3 6 5 7 10 8 1 01 2 000 21 264 00 0 1 25 0 1 25 000 000 000 0 0 25 0 0 25 000 0 0 25 0 0 25 000 0 0 25 0 0 25 000 9 15 25 04 9 6 9 13 01 0 000 409 TABLE 38 Incidence of Microscopic Observations Terminal Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS Covance 6329-183 3M T-6295 PAGE: 10 TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,5;WEEKS=ALL DEATH=T;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E ' T E D -- SEX: -MALE- FEMALE GROUP: -1 - -2 - -3- -4- -5- -1 - -2 - -3- -4- -5- ORGAN AND FINDING DESCRIPTION NUMBER: 11 10 17 25 22 24 15 9 15 25 -------------------------------------- ------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** THYROID (TY) ....................... .................. NUMBER EXAMINED: 11 10 17 24 22 24 15 NOT REMARKABLE: 4 3 5 5 6 10 4 9 15 25 04 9 --B-ADENOMA, C-CELL --B-ADENOMA, FOLLICULAR CELL --HYPERTROPHY, FOLLICULAR CELL --M-CARCINOMA, C-CELL --X-HEMATOPOIETIC NEOPLASIA (SEE --B-HIBERNOMA --COLLOID, DECREASED "HEMATO NEOPLASIA" FOR TYPE) 1 23936321 4 02 2 2 4 0001 1 001 0000000 001 0001 000 00001 00000 0000000001 00000001 00 PARATHYROID (PT) ................... .................. NUMBER EXAMINED: 11 10 17 24 22 24 14 NOT REMARKABLE: 9 9 16 19 20 24 14 9 15 24 9 14 23 --HYPERPLASIA --B-ADENOMA 1 1 04 2 0001 0 1 01 1 000001 PITUITARY (PI) ..................... .................. NUMBER EXAMINED: 11 NOT REMARKABLE: 2 5 13 11 22 24 15 0002 1 0 6 11 25 004 --B-ADENOMA --HYPERPLASIA --ANGIECTASIS --CYST --M-CARCINOMA 7 2 11 11 17 16 10 4 7 17 3 3 4 2 6 10 6 2 4 6 0000001 1 02 001 1 00001 2 000000001 0 410 TABLE 38 Incidence of Microscopic Observations Terminal Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS Covance 6329-183 3M T-6295 PAGE: 11 TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,5;WEEKS=ALL DEATH=T;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R - O F - A N I M A L S - A F F E T E D -- SEX: -MALE-- FEMALE GROUP: -1 - -2 - -3- -4- -5- -1 - -2 - -3- -4- -5- NUMBER: 11 10 17 25 22 24 15 9 15 25 HARDERIAN GLAND (HG) ............... .................. NUMBER EXAMINED: 11 NOT REMARKABLE: 9 01 01 0 22 24 0 20 21 0 0 0 25 0 0 0 22 --INFILTRATE, LYMPHOHISTIOCYTIC 2 0002 3 0003 SALIV GL, MANDIB (SG) .............. .................. NUMBER EXAMINED: 11 NOT REMARKABLE: 11 0 0 0 22 24 0 0 0 22 24 0 0 0 25 0 0 0 25 STOMACH, GL (ST) ............................... ...... NUMBER EXAMINED: 11 6 6 5 22 24 21 0 25 NOT REMARKABLE: 7 1 0 3 14 23 2 0 0 23 --EDEMA --INFLAMMATION, ACUTE --EROSION --INFLAMMATION, CHRONIC-ACTIVE --ULCERATION --HYPERPLASIA, EPITHELIAL, GLANDULAR --MINERALIZATION, GLANDULAR MUCOSA --FIBROSIS 01 00000000 01 1 001 01 01 0002 2 001 01 2 01 1 1 00001 001 0000001 44 6 06 00000 00001 00000 00001 00000 STOMACH, NONGL (SU) ............................ ...... NUMBER EXAMINED: 11 NOT REMARKABLE: 11 0 0 0 22 24 0 0 0 22 24 1 0 0 25 0 0 0 24 --ACANTHOSIS / HYPERKERATOSIS --ULCERATION --INFLAMMATION, CHRONIC-ACTIVE 0000001 001 0000000001 0000001 001 411 TABLE 38 Incidence of Microscopic Observations Terminal Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS Covance 6329-183 3M T-6295 PAGE: 12 TABLE INCLUDES: SEX=ALL;GROUP=1, 2,3,4,5;WEEKS=ALL DEATH=T;FIND=ALL ;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R - O F - A N I M A L S - A F F E T E D -- SEX: -MALE-- FEMALE GROUP: -1 - -2 - -3- -4- -5- -1 - -2 - -3- -4- -5- NUMBER: 11 10 17 25 22 24 15 9 15 25 DUODENUM (DU) .... ..................................... NUMBER EXAMINED: NOT REMARKABLE: --INFLAMMATION, CHRONIC-ACTIVE JEJUNUM (JE) .... ..................................... NUMBER EXAMINED: NOT REMARKABLE: 11 10 1 11 11 0 0 0 22 24 0 0 0 25 0 0 0 22 24 0 0 0 25 000000000 0 0 0 22 24 0 0 0 25 0 0 0 22 24 0 0 0 25 ILEUM (IL) ...... ..................................... NUMBER EXAMINED: 11 NOT REMARKABLE: 10 0 0 0 22 24 0 0 0 22 23 0 0 0 25 0 0 0 23 --INFLAMMATION, CHRONIC-ACTIVE --HYPERPLASIA, GALT (GUT ASSOCIATED LYMPHOID TISSUE) 1 000000001 000001 0001 CECUM (CE) ...... ..................................... NUMBER EXAMINED: 11 NOT REMARKABLE: 10 0 0 0 22 24 0 0 0 22 24 0 0 0 25 0 0 0 24 --INFLAMMATION, CHRONIC-ACTIVE 1 000000001 COLON (CO) ...... ..................................... NUMBER EXAMINED: 11 0 0 0 22 24 1 0 0 25 NOT REMARKABLE: 8 0 0 0 22 23 0 0 0 24 --PARASITISM --INFLAMMATION, CHRONIC-ACTIVE --DIVERTICULUM --B-LEIOMYOMA 2 000000000 1 000000001 000001 0000 0000001 000 412 TABLE 38 Incidence of Microscopic Observations Terminal Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS Covance 6329-183 3M T-6295 PAGE: 13 TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,5;WEEKS=ALL DEATH=T;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R - O F - A N I M A L S - A F F E T E D -- SEX: -MALE-- FEMALE GROUP: -1 - -2 - -3- -4- -5- -1 - -2 - -3- -4- -5- NUMBER: 11 10 17 25 22 24 15 9 15 25 RECTUM (RE) ............................................ NUMBER EXAMINED: 11 0 0 0 22 23 0 0 0 25 NOT REMARKABLE: 9 0 0 0 21 23 0 0 0 20 --PARASITISM --INFLAMMATION, CHRONIC-ACTIVE 1 0001 00004 1 000000001 SKIN (SK) .............................................. NUMBER EXAMINED: 11 3 4 6 22 24 1 0 0 25 NOT REMARKABLE: 10 0 0 0 15 23 0 0 0 21 --ACANTHOSIS/HYPERKERATOSIS --INFLAMMATION, CHRONIC-ACTIVE --ULCERATION --HEMORRHAGE --CYST, EPIDERMAL INCLUSION --SUPERFICIAL CRUSTING --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" --B-KERATOACANTHOMA --M-FIBROSARCOMA --B-FIBROMA --FIBROSIS --B-ADENOMA, BASAL CELL/SQUAMOUS/SEBACEOUS --B-LIPOMA --M-CARCINOMA, SQUAMOUS CELL --EDEMA FOR TYPE) 1 001 000001 1 1 01 2 01 001 0001 000001 0000001 000 1 0002 00001 1 0001 00000 00001 00000 02 1 3 3 00000 0001 000000 01 2 1 000001 01 01 1 00000 001 001 0000 00001 01 001 01 00000000 00001 00000 413 TABLE 38 Incidence of Microscopic Observations Terminal Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS Covance 6329-183 3M T-6295 PAGE: 14 TABLE INCLUDES: SEX=ALL;GROUP=1,2,3/4 /5;WEEKS=ALL DEATH=T;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E ' T E D -- SEX: -MALE- FEMALE GROUP: -1 - -2 - -3- -4- -5- -1 - -2 - -3- -4- -5- ORGAN AND FINDING DESCRIPTION ----------------------------------------- -- NUMBER: 11 10 17 25 22 24 15 9 15 25 -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- MAMMARY, FEMALE (MF) ................. ................ NUMBER EXAMINED: 0 0 0 0 0 24 15 9 15 25 NOT REMARKABLE: 0 0 0 0 0 0 1 1 0 1 --M-CARCINOMA --CYST / GALACTOCELE --INFLAMMATION, CHRONIC-ACTIVE --B-FIBROADENOMA --HYPERPLASIA, GLANDULAR --M-FIBROSARCOMA --B-ADENOMA --FIBROSIS 0000051 1 1 6 0 0 0 0 0 13 9 3 6 11 000002 0000 0 0 0 0 0 12 9 5 6 7 0 0 0 0 0 19 10 6 12 21 000000001 1 0000032 1 2 1 000001 0000 URINARY BLADDER (UB) ................. ................ NUMBER EXAMINED: 11 10 17 25 22 24 15 NOT REMARKABLE: 10 5 13 17 17 23 11 9 15 25 9 15 23 --INFILTRATE, LYMPHOHISTIOCYTIC --PROTEINACEOUS MATERIAL, LUMINAL --HYPERPLASIA, TRANSITIONAL CELL --INFLAMMATION, CHRONIC-ACTIVE --DIVERTICULUM 03 1 4 004 002 1 2344 00000 001 31 00000 0001 000000 000001 0000 UTERUS (UT) .......................... ................ NUMBER EXAMINED: 0 0 0 0 0 24 4 3 6 25 NOT REMARKABLE: 0 0 0 0 0 14 01 0 13 --DILATATION --B-POLYP, ENDOMETRIAL, STROMAL --HYPERPLASIA, CYSTIC, ENDOMETRIAL --HEMORRHAGE 000004 1 02 2 0000001 003 00000622 38 000000001 0 414 TABLE 38 Incidence of Microscopic Observations Terminal Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS Covance 6329-183 3M T-6295 PAGE: 15 TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,5;WEEKS=ALL DEATH=T;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E ' T E D -- SEX: -MALE-- FEMALE GROUP: -1 - -2 - -3- -4- -5- -1 - -2 - -3- -4- -5- ORGAN AND FINDING DESCRIPTION NUMBER: 11 10 17 25 22 24 15 9 15 25 ** FROM PREVIOUS PAGE ** UTERUS (UT) ........................ .................. NUMBER EXAMINED: 0 0 0 0 0 24 4 3 6 25 NOT REMARKABLE: 0 0 0 0 0 14 01 0 13 --B-ADENOMA 000000001 0 CERVIX (CV) ........................ .................. NUMBER EXAMINED: 0 0 0 0 0 24 0 2 2 25 NOT REMARKABLE: 0 0 0 0 0 21 0 0 0 21 --HYPERPLASIA, MUCINOUS --HYPERPLASIA, STROMAL --M-SARCOMA, STROMAL --HYPERPLASIA, CYSTIC --HEMORRHAGE 000003 01 1 2 00000001 00 0000000002 000000001 0 0000000001 VAGINA (VA) ........................ .................. NUMBER EXAMINED: 0 0 0 0 0 24 0 1 1 25 NOT REMARKABLE: 0 0 0 0 0 9 0 0 0 13 --LUMEN, EXUDATE --HYPERPLASIA, MUCINOUS --I-CARCINOMA 000004 0004 0 0 0 0 0 11 0 1 1 8 000001 0000 PROSTATE (PR) ...................... .................. NUMBER EXAMINED: 11 1 5 4 22 0 0 0 0 0 NOT REMARKABLE: 2 0 0 0 5 0 0 0 0 0 --INFLAMMATION, CHRONIC-ACTIVE --HYPERPLASIA, EPITHELIAL 9 1 5 4 17 0 0 0 0 0 0001 000000 415 TABLE 38 Incidence of Microscopic Observations Terminal Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS Covance 6329-183 3M T-6295 PAGE: 16 TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,5;WEEKS=ALL DEATH=T;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R - O F - A N I M A L S - A F F E T E D -- SEX: -MALE- FEMALE GROUP: -1- -2- -3- -4- -5- -1- -2- -3- -4- -5- NUMBER: 11 10 17 25 22 24 15 9 15 25 11SEMINAL VESICLES (SV) ............... ................. NUMBER EXAMINED: 8NOT REMARKABLE: 00 11 10 22 18 00 00 --SECRETION, DECREASED --SECRETION, INCREASED --INFLAMMATION, CHRONIC-ACTIVE 210 000 000 100 10 3 000 000 11EPIDIDYMIDES (EP) ................... ................. NUMBER EXAMINED: NOT REMARKABLE: 9 00 00 00 22 19 00 00 --INFILTRATE, LYMPHOHISTIOCYTIC --HYPOSPERMIA --VACUOLATION, TUBULAR EPITHELIUM 200 000 000 000 0 31 000 000 11HEMATO NEOPLASIA (HN) ............... ................. NUMBER EXAMINED: 11NOT REMARKABLE: 00 00 00 2220 24 24 00 --M-LYMPHOMA --M-SARCOMA, HISTIOCYTIC 00 00 00 00 11 00 00 CAUSE OF DEATH (CD) ................. ................. NUMBER EXAMINED: NOT REMARKABLE: 110 100 170 250 220 240 150 --SCHEDULED SACRIFICE 11 10 17 25 22 24 15 00 00 00 000 000 000 00 00 00 000 000 000 00 00 25 25 00 00 00 90 150 250 9 15 25 416 TABLE 38 Incidence of Microscopic Observations Terminal Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS Covance 6329-183 3M T-6295 PAGE: 17 TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,5;WEEKS=ALL DEATH=T;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R - O F - A N I M A L S - A F F E C T E D -- SEX: -MALE- FEMALE- GROUP: -1 - -2 - -3- -4- -5- -1 - -2 - -3- -4- -5- NUMBER: 11 10 17 25 22 24 15 9 15 25 LN, MEDIASTINAL (ML) .................................. NUMBER EXAMINED: 0 0 0 2 01 0002 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 --HEMORRHAGE --HYPERPLASIA, LYMPHOPLASMACYTIC --LYMPHANGIECTASIS 0000000001 0001 01 0001 0002 000001 LN, AXILLARY (AX) ..................................... NUMBER EXAMINED: 4 2 2 3 6 7 4 2 5 6 NOT REMARKABLE: 3 0 0 2 4 3 0 1 1 2 --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) --HEMORRHAGE --HYPERPLASIA, LYMPHOPLASMACYTIC --INFILTRATE, MACROPHAGE, PIGMENTED 00001 00000 001 1 1 2 001 1 02 2 1 1 2 4 1 4 4 1 001 01 2 01 0 LN, INGUINAL (IN) ..................................... NUMBER EXAMINED: 0 1 NOT REMARKABLE: 0 1 0003 0002 1 001 0000 --HYPERPLASIA, LYMPHOPLASMACYTIC --INFILTRATE, MACROPHAGE, PIGMENTED --HEMORRHAGE --N-CARCINOMA 0000001 000 0000000001 0000000001 000001 0000 LN, OTHER (LN) ........................................ NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 1 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 --HEMORRHAGE --INFILTRATE, MACROPHAGE, PIGMENTED 0000000001 0000000001 417 TABLE 38 Incidence of Microscopic Observations Terminal Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS Covance 6329-183 3M T-6295 PAGE: 18 TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,5;WEEKS=ALL DEATH=T;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R - O F - A N I M A L S - A F F E T E D -- SEX: MALE-- FEMALE GROUP: -1 - -2 - -3- -4- -5- -1 - -2 - -3- -4- -5- NUMBER: 11 10 17 25 22 24 15 9 15 25 LN, RENAL (RL) --HYPERPLASIA, LYMPHOPLASMACYTIC --HEMORRHAGE --INFILTRATE, MACROPHAGE, PIGMENTED --DILATATION, SINUSOIDAL LN, MANDIBULAR (MN) .................. --HYPERPLASIA, LYMPHOPLASMACYTIC --HEMORRHAGE --LYMPHANGIECTASIS --INFILTRATE, MACROPHAGE, PIGMENTED VERTEBRA/MARROW (VT) ................. NUMBER EXAMINED: 0 1 0 1 2 2 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 0001 01 0000 01 002 1 0000 000001 0000 00002 00000 NUMBER EXAMINED: 2 1 1 2 2 5 2 0 3 3 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 2 2 1 1 1 2 3 1 02 1 1 001 2 51 01 0 1 000000000 00001 00000 NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 MAMMARY, MALE (MM) --GALACTOCELE --INFLAMMATION, CHRONIC-ACTIVE --B-FIBROADENOMA --M-CARCINOMA --B-ADENOMA NUMBER EXAMINED: 2 1 1 1 2 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 1 1 1 01 00000 2 000000000 0001 1 00000 1 000000000 00001 00000 418 TABLE 38 Incidence of Microscopic Observations Terminal Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS Covance 6329-183 3M T-6295 PAGE: 19 TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,5;WEEKS=ALL DEATH=T;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R - O F - A N I M A L S - A F F E T E D -- SEX: -MALE-- FEMALE GROUP: -1 - -2 - -3- -4- -5- -1 - -2 - -3- -4- -5- NUMBER: 11 10 17 25 22 24 15 9 15 25 COAGULATING GL (CG) ................... ............... NUMBER EXAMINED: 1 000000000 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 --INFLAMMATION, CHRONIC-ACTIVE 1 000000000 TAIL (TI) .............................. ............... NUMBER EXAMINED: 3 001 00001 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 --INFLAMMATION, CHRONIC-ACTIVE --CYST, EPIDERMAL INCLUSION --ACANTHOSIS / HYPERKERATOSIS --B-KERATOACANTHOMA --M-LIPOSARCOMA 000000001 0 2 000000000 1 000000000 0001 000000 000000001 0 LN, LUMBAR/ILIAC (LM) ................. ............... NUMBER EXAMINED: 2 0 1 4 0 1 1 2 4 7 NOT REMARKABLE: 0 0 0 0 0 0 0 0 2 1 --LYMPHANGIECTASIS --HYPERPLASIA, LYMPHOPLASMACYTIC --HEMORRHAGE --INFILTRATE, MACROPHAGE, PIGMENTED --N-CARCINOMA 1 01 3 000000 001 3 001 22 5 1 000000002 1 00000001 0 000001 0000 CAVITY, ABDOM (PC) .................... ............... NUMBER EXAMINED: 1 0 0 1 0 1 1 0 0 3 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 --INFLAMMATION / NECROSIS, FAT --INFLAMMATION, CHRONIC-ACTIVE 1 000001 003 1 000000000 419 TABLE 38 Incidence of Microscopic Observations Terminal Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS Covance 6329-183 3M T-6295 PAGE: 20 TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,5;WEEKS=ALL DEATH=T;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E T E D -- SEX: -MALE-- FEMALE GROUP: -1 - -2 - -3- -4- -5- -1 - -2 - -3- -4- -5- ORGAN AND FINDING DESCRIPTION NUMBER: 11 10 17 25 22 24 15 9 15 25 ** FROM PREVIOUS PAGE ** CAVITY, ABDOM (PC) ................. .................. NUMBER EXAMINED: 1 0 0 1 0 1 1 0 0 3 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 --INFLAMMATION, VASCULAR --THROMBUS --M-LIPOSARCOMA 0001 000000 0001 000000 000001 0000 AUDITORY SEB GL (AS) ............... .................. NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 PREPUTIAL GLAND (PG) ....................... ......... NUMBER EXAMINED: 0 0 1 0000000 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 --INFLAMMATION, CHRONIC-ACTIVE --ECTASIA, DUCTAL 001 001 0000000 0000000 FOOT/FOOTPAD (FP) .......................... ......... NUMBER EXAMINED: 0 2 3 3 0 0 2 1 0 4 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 --INFLAMMATION, CHRONIC-ACTIVE --ULCERATION --ACANTHOSIS / HYPERKERATOSIS --FIBROSIS --BACTERIA --HYPERPLASIA, BONE 02 3 3 002 1 04 02 32 001 1 02 01 3 3 002 1 03 0000001 001 01 01 000001 0000000001 420 TABLE 38 Incidence of Microscopic Observations Terminal Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS Covance 6329-183 3M T-6295 PAGE: 21 TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,5;WEEKS=ALL DEATH=T;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION N U M B E R - O F - A N I M A L S - A F F E < T E D -- SEX: -MALE-- FEMALE GROUP: -1 - -2 - -3- -4- -5- -1 - -2 - -3- -4- -5- NUMBER: 11 10 17 25 22 24 15 9 15 25 AORTA (AO) ............................. ............... NUMBER EXAMINED: 0 0 0 0 1 00000 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 --MINERALIZATION 00001 00000 CALVARIUM/SKULL (CA) .................. ............... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 LN, ANT MES/PANC (AP) ................. ............... NUMBER EXAMINED: 0 0 1 01 NOT REMARKABLE: 0 0 0 0 1 00000 00000 --HEMORRHAGE --HYPERPLASIA, LYMPHOPLASMACYTIC --INFILTRATE, MACROPHAGE, PIGMENTED 001 001 001 0000000 0000000 0000000 HEAD, CORONAL (HC) .................... ............... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 JOINT, OTHER (JT) ..................... ............... NUMBER EXAMINED: 1 1 1 1 0 0 0 0 0 1 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 --INFLAMMATION, CHRONIC-ACTIVE --FIBROSIS 1 1 1 1 000001 1 000000000 421 TABLE 38 Incidence of Microscopic Observations Terminal Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS Covance 6329-183 3M T-6295 PAGE: 22 TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,5;WEEKS=ALL DEATH=T;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION N U M B E R - O F - A N I M A L S - A F F E < T E D -- SEX: -MALE-- FEMALE GROUP: -1 - -2 - -3- -4- -5- -1 - -2 - -3- -4- -5- NUMBER: 11 10 17 25 22 24 15 9 15 25 CLITORAL GLAND (CL) ................ .................. NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 URETER (UE) ........................ .................. NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 ADIPOSE TISSUE (AT) ................ .................. NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 DIAPHRAGM (DP) ..................... .................. NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 CAVITY, THORACIC (TA) .............. .................. NUMBER EXAMINED: 1 000000000 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 --B-AORTIC BODY TUMOR 1 000000000 LN, TRACHEOBRON (TB) ............... .................. NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 LN, POPLITEAL (PP) ................. .................. NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 422 TABLE 38 Incidence of Microscopic Observations Terminal Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS Covance 6329-183 3M T-6295 PAGE: 23 TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,5;WEEKS=ALL DEATH=T;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION N U M B E R - O F - A N I M A L S - A F F E < T E D -- SEX: -MALE-- FEMALE GROUP: -1 - -2 - -3- -4- -5- -1 - -2 - -3- -4- -5- NUMBER: 11 10 17 25 22 24 15 9 15 25 MUSCLE, OTHER (MO) ................. .................. NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 PERICARDIAL SAC (PD) ............... .................. NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 PINNA (PN) ......................... .................. NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 ARTERY, OTHER (AU) ................. .................. NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 AORTIC ARCH (AA) ................... .................. NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 LN, ILEOCECAL (IC) ................. .................. NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 VAS DEFERENS (VS) .................. .................. NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 423 TABLE 38 Incidence of Microscopic Observations Terminal Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS Covance 6329-183 3M T-6295 PAGE: 24 TABLE INCLUDES: SEX=ALL;GROUP=1,2,3/4 /5;WEEKS=ALL DEATH=T;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION N U M B E R - O F - A N I M A L S - A F F E < T E D -- SEX: MALE-- FEMALE GROUP: -1 - -2 - -3- -4- -5- -1 - -2 - -3- -4- -5- NUMBER: 11 10 17 25 22 24 15 9 15 25 BILE DUCT (BD) ..................... .................. NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 TRIGEMINAL NERVE (TG) .............. .................. NUMBER EXAMINED: 0 0 0 1 000000 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 --GANGLION ** END OF LIST ** 0001 000000 424 TABLE 39 Incidence of Microscopic Observations Unscheduled Deaths and Sacrifices 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS Covance 6329-183 3M T-6295 PAGE: 1 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C T E D -- SEX: --MALE--- --FEMALE--- GROUP: -1 - -2 - -3- -4- -5- -6- -1 - -2 - -3- -4- -5- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 39 40 33 25 29 30 26 35 41 35 25 23 ** TOP OF LIST ** EYE (EY) ................................... .......... NUMBER EXAMINED: 39 39 33 25 29 30 26 35 41 35 25 23 NOT REMARKABLE: 34 36 28 24 26 28 23 30 37 30 21 22 --DYSTROPHY, CORNEAL IN EARLY STAGES, CONSISTS PRIMARILY OF FOCAL MINERALIZATION --INFLAMMATION, ACUTE --I-NEUROFIBROSARCOMA --ATROPHY, RETINAL --DEGENERATION, LENTICULAR --INFLAMMATION, CHRONIC-ACTIVE --UNILATERALLY EXAMINED --ULCERATION, CORNEAL 41 31 2231 231 0 1 0001 02 3 1 2 3 1 001 000000000 001 0000001 00 01 000001 0000 01 0000001 01 0 000000001 000 000000001 01 0 BRAIN (BR) ................................. .......... NUMBER EXAMINED: 39 40 33 25 29 30 26 35 41 35 25 23 NOT REMARKABLE: 18 19 6 5 15 16 7 9 6 9 8 8 --DILATATION, VENTRICULAR --M-OLIGODENDROGLIOMA --COMPRESSION, VENTRAL --HEMORRHAGE --M-ASTROCYTOMA --DEGENERATION, FOCAL --MINERALIZATION --INFILTRATE, MACROPHAGE, PIGMENTED --THROMBUS --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" --M-GRANULAR CELL TUMOR --I-CARCINOMA FOR TYPE) 13 12 14 10 7 6 14 12 19 11 13 10 1 00000000000 18 15 22 18 12 13 19 26 35 23 16 15 2 01 1 1 031 1 1 01 2 001 000001 00 001 000000000 01 31 1 0001 1 00 1 2 00001 1 02 01 00000001 0000 1 00000000000 01 0000000000 000000001 1 00 425 TABLE 39 Incidence of Microscopic Observations Unscheduled Deaths and Sacrifices 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS Covance 6329-183 3M T-6295 PAGE: 2 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C T E D -- SEX: --MALE--- --FEMALE--- GROUP: -1 - -2 - -3- -4- -5- -6- -1 - -2 - -3- -4- -5- -6- ORGAN AND FINDING DESCRIPTION -- NUMBER: 39 40 33 25 29 30 26 35 41 35 25 23 ----------------- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** BRAIN (BR) ............................................. NUMBER EXAMINED: 39 40 33 25 29 30 26 35 41 35 25 23 NOT REMARKABLE: 18 19 6 5 15 16 7 9 6 9 8 8 --M-MENINGEAL SARCOMA --M-NEUROFIBROSARCOMA 01 0000000000 001 000000000 LIVER (LI) ............................................. NUMBER EXAMINED: 39 40 33 25 29 30 26 35 41 35 25 23 NOT REMARKABLE: 2 0 0 0 0 1 2 1 1 1 0 0 --INFILTRATE, LYMPHOHISTIOCYTIC --HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED --GRANULAR CYTOPLASM, EOSINOPHILIC, CENTRILOBULAR --PIGMENT, HEPATOCELLULAR, CENTRILOBULAR --NECROSIS, INDIVIDUAL HEPATOCYTE --VACUOLATION, HEPATOCELLULAR, MIDZONAL / CENTRILOBULAR --VACUOLATION, HEPATOCELLULAR, PERIPORTAL --FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL --FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC --DEGENERATION, CYSTIC --NECROSIS, COAGULATIVE --CONGESTION --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) --HEMATOPOIESIS, EXTRAMEDULLARY --HYPERPLASIA, BILE DUCT --FIBROSIS, PERIBILIARY --HYPERTROPHY, HEPATOCELLULAR, PERIPORTAL --INFILTRATE, MACROPHAGE, PIGMENTED 26 28 26 17 15 22 16 23 27 24 19 16 0 1 1 4 11 3 1 1 3 8 19 2 00005 0 00003 0 21 2233 231 323 10 11 7 12 10 7 6 14 13 4 7 11 1 441 23 2 11 11 4 8 12 1 31 61 3 111110 2 2 002 1 336245 11 15 17 11 10 13 457361 31 5223 453432 0 0 0 1 16 0 0 0 0 13 22235 1 001 0 5 10 13 10 9 9 15 11 86 5 9 8 11 5 01 02 3 3221 0 1 21 00 001 1 0 8 15 11 41 7 15 16 10 13 26265 3 982 1 1 3559 1 0 1 1 5 5 4 0 1 0 1 4 8 1 3 6 426 TABLE 39 Incidence of Microscopic Observations Unscheduled Deaths and Sacrifices 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS Covance 6329-183 3M T-6295 PAGE: 3 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C T E D -- SEX: --MALE--- --FEMALE--- GROUP: -1 - -2 - -3- -4- -5- -6- -1 - -2 - -3- -4- -5- -6- ORGAN AND FINDING DESCRIPTION -- NUMBER: 39 40 33 25 29 30 26 35 41 35 25 23 -- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** LIVER (LI) ...................................... ..... NUMBER EXAMINED: 39 40 33 25 29 30 26 35 41 35 25 23 NOT REMARKABLE: 2 0 0 0 0 1 2 1 1 1 0 0 --VACUOLATION, HEPATOCELLULAR --DEGENERATION / NECROSIS, MIDZONAL --DEGENERATION / NECROSIS, CENTRILOBULAR --HEMORRHAGE --CYST, BILIARY --INFLAMMATION, SUBACUTE --GLYCOGEN, HEPATOCELLULAR, INCREASED --ANOMALY, SECONDARY TO DIAPHRAGMATIC HERNIA --ANGIECTASIS --LEUKOCYTOSIS, SINUSOIDAL --B-ADENOMA, HEPATOCELLULAR --INFLAMMATION, CHRONIC-ACTIVE --ECTASIA, BILE DUCT --STASIS, BILE --MITOSIS, HEPATOCELLULAR, INCREASED --LIPIDOSIS, SUBCAPSULAR (TENSION LIPIDOSIS) --N-SARCOMA --B-CHOLANGIOMA --N-CARCINOMA --KARYOMEGALY --M-CARCINOMA, HEPATOCELLULAR 1 1 3 1 4 1 02 03 1 1 00001 0000000 1 0005 1 01 02 2 1 001 00001 01 00 01 01 01 2 1 1 1 1 0 01 000000001 0 1 3 001 0000000 001 000000001 1 1 1 01 02 0001 0 00001 001 0000 02 2 1 1 0001 01 1 0001 1 1 0001 1 0 00000000001 0 00000000001 0 00001 001 1 000 001 00001 001 0 000001 000000 0000000001 00 00001 0000000 01 0000000000 00000000001 0 LIVER-OIL RED O (LI0) .......................... ..... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 0 0 427 TABLE 39 Incidence of Microscopic Observations Unscheduled Deaths and Sacrifices 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS Covance 6329-183 3M T-6295 PAGE: 4 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---SEX: ------------ MALE------------ ----------- FEMALE GROUP: -1 - -2 - -3- -4- -5- -6- -1 - -2 - -3- -4- -5- -6- ORGAN AND FINDING DESCRIPTION -- NUMBER: 39 40 33 25 29 30 26 35 41 35 25 23 ----------------- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- KIDNEY (KD) ............................................ NUMBER EXAMINED: 39 40 33 25 29 30 26 35 41 35 25 23 NOT REMARKABLE: 1 3 0 2 0 3 1 5 4 0 1 0 --NEPHROPATHY, CHRONIC PROGRESSIVE --INFILTRATE, LYMPHOHISTIOCYTIC --DILATATION, PELVIC --MINERALIZATION, PELVIC --REGENERATION, TUBULAR EPITHELIUM --MINERALIZATION, TUBULAR --HEMATOPOIESIS, EXTRAMEDULLARY --ATROPHY, TUBULAR --VACUOLATION, TUBULAR EPITHELIUM --N-CARCINOMA --PIGMENT, TUBULAR --KARYOMEGALY, TUBULAR CELL --HYALINE DROPLETS, TUBULAR EPITHELIUM --INFILTRATE, MACROPHAGE, PIGMENTED --HYPERPLASIA, TRANSITIONAL CELL --INFLAMMATION, SUPPURATIVE, PELVIC --INFLAMMATION, CHRONIC-ACTIVE, PELVIC --NECROSIS, PAPILLARY --ECTASIA, TUBULAR --CAST, PROTEINACEOUS --CYST --INFLAMMATION, CHRONIC-ACTIVE --FIBROSIS, CORTICAL --ANGIECTASIS --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 30 33 33 21 22 27 11 18 18 18 17 13 1 0001 001 1 1 1 0 3 3 3 1 6 7 02 3 01 1 9 7 7 5 5 4 23 23 30 29 14 18 1 2 01 1 1 01 0000 2 00031 76 97 95 01 000001 1 1 00 000000001 000 00002 001 02 1 0 000000000001 3 1 001 0002 1 3 1 00000001 0000 51 01 2 0000000 2 01 00000001 0 02 2 2 1 3 3 87 62 2 02 32 02 02 4 1 2 1 1 2 2 1 1 1 01 002 1 00002 0000000 1 1 000000001 0 00000000001 0 01 0001 01 1 000 1 001 00000000 1 00000000000 000001 000000 1 00000000000 428 TABLE 39 Incidence of Microscopic Observations Unscheduled Deaths and Sacrifices 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS Covance 6329-183 3M T-6295 PAGE: 5 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C T E D -- SEX: ---MALE--- --FEMALE--- GROUP: -1- -2- -3- -4- -5- -6- -1- -2- -3- -4- -5- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 39 40 33 25 29 30 26 35 41 35 25 23 ---------------------------------------------------------- ----------------- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** KIDNEY (KD) ............................................ NUMBER EXAMINED: 39 40 33 25 29 30 26 35 41 35 25 23 1 0 2 0 1 0 1 0NOT REMARKABLE: 3 3 54 --BACTERIAL COLONIES --DEGENERATION, CYSTIC, UNILATERAL --INFILTRATE, FAT, INTERSTITIAL --N-SARCOMA --M-CARCINOMA, TUBULAR CELL --ULCERATION, TRANSITIONAL EPITHELIUM, --M-LIPOSARCOMA --THROMBUS --B-LIPOMA --THROMBUS, GLOMERULAR --B-PAPILLOMA, TRANSITIONAL CELL --DEGENERATION TUBULAR PELVIC 110000000000 000000000000 100000000000 100000000000 110000000000 111000000000 000000000000 000000000000 110000000000 110000000000 000000000000 000000000000 SPLEEN (SP) ............................................ NUMBER EXAMINED: 39 40 33 25 29 30 26 35 41 35 25 23 2 2 6 2 0 0 0 0NOT REMARKABLE: 5 4 3 3 --PIGMENT, INCREASED --HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" --DEPLETION, LYMPHOCYTIC --FIBROSIS, CAPSULAR --CYST, SEROSAL --M-OSTEOSARCOMA --HYPERPLASIA, LYMPHORETICULAR --M-HEMANGIOSARCOMA FOR TYPE) 21301100000 27 161000000 22 150000000 16 90000000 11261100000 11241100000 18 131000000 18 230000000 31 161100000 23 151000000 18 41000000 14 151100000 429 TABLE 39 Incidence of Microscopic Observations Unscheduled Deaths and Sacrifices 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS Covance 6329-183 3M T-6295 PAGE: 6 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C T E D -- SEX: ---MALE--- --FEMALE--- GROUP: -1 - -2 - -3- -4- -5- -6- -1 - -2 - -3- -4- -5- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 39 40 33 25 29 30 26 35 41 35 25 23 ---------------------------------------------------------- ----------------- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** SPLEEN (SP) ............................................ NUMBER EXAMINED: 39 40 33 25 29 30 26 35 41 35 25 23 NOT REMARKABLE: 5 4 2 3 2 6 2 0 0 0 3 0 --HEMORRHAGE --NECROSIS 00000001 00000001 0000 0000 PANCREAS (PA) ......................................... NUMBER EXAMINED: 39 39 33 25 29 30 26 35 41 35 25 23 NOT REMARKABLE: 11 11 6 6 8 7 16 17 21 23 15 10 --ZYMOGEN GRANULES, DECREASED --INFILTRATE, LYMPHOHISTIOCYTIC --INFILTRATE, MACROPHAGE, PIGMENTED --HYPERPLASIA, ISLET CELLS --ATROPHY, ACINAR --HYPERTROPHY / BASOPHILIA, ACINAR CELL --M-CARCINOMA, ISLET CELL --B-ADENOMA, ISLET CELL --B-ADENOMA, ACINAR CELL --VACUOLATION, FOCAL --INFILTRATION, FAT, INTERSTITIAL --INFLAMMATION, VASCULAR --THROMBUS --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" --INFILTRATE, EOSINOPHILIC --FIBROSIS --INFLAMMATION, GRANULOMATOUS --NECROSIS FOR TYPE) 9 14 8 8 8 7 2 9 13 7 8 8 1 00001 1 02 2 1 1 16 15 14 10 11 13 0 2 1 1 0 0 1 1 21 1 1 21 0000 44552221 41 1 3 1 2 01 1 1 04 2 001 1 2 1 01 2 1 01 01 0 42222 001 0000 01 0000000000 00000001 0000 5 10 7 4 6 3 4 5 2 2 1 3 1 1 4 1 03 01 1 001 1 00002 000000 000001 001 000 001 000000000 001 0000001 00 001 000000000 00001 0000000 430 TABLE 39 Incidence of Microscopic Observations Unscheduled Deaths and Sacrifices 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS Covance 6329-183 3M T-6295 PAGE: 7 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---SEX: ------------ MALE------------ ----------- FEMALE GROUP: -1 - -2 - -3- -4- -5- -6- -1 - -2 - -3- -4- -5- -6- ORGAN AND FINDING DESCRIPTION -- NUMBER: 39 40 33 25 29 30 26 35 41 35 25 23 -- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ADRENAL, CORTEX (AC) .................................. NUMBER EXAMINED: 39 40 33 25 29 30 26 35 41 35 25 23 NOT REMARKABLE: 10 5 8 7 9 8 1 2 3 2 1 1 --VACUOLATION, FOCAL --UNILATERALLY EXAMINED --CONGESTION --ANGIECTASIS --HEMATOPOIESIS, EXTRAMEDULLARY --INFILTRATE, MACROPHAGE, PIGMENTED --HEMORRHAGE --HYPERTROPHY, CORTICAL CELL --VACUOLATION, DIFFUSE --DEGENERATION, CYSTIC / HEMORRHAGIC --HYPERPLASIA, CORTICAL --PIGMENT --MINERALIZATION --ATROPHY, CORTICAL --B-ADENOMA --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" --DEGENERATION / NECROSIS, UNILATERAL --NECROSIS, FOCAL --FIBROSIS --N-SARCOMA --M-CARCINOMA FOR TYPE) 7 14 10 3 7 7 4 0 5 3 2 3 1 00001 01 01 00 1 000001 001 00 2 2 3 1 1 2 7 15 24 14 7 9 1 01 0031 5351 0 000000001 03 0 00000001 02 00 5 5 3 2 5 7 10 13 10 13 8 11 1 21 1 00000000 1 3 2 0 1 0 10 13 8 14 6 8 2 2 2 2 1 1 04 34 32 14 26 16 14 13 14 14 23 27 21 17 13 1 00001 000000 01 1 1 1 001 01 00 2 01 2 001 1 1 1 1 0 1 00000000000 001 01 001 1 01 0 001 000000000 001 001 00001 0 000001 000000 00001 001 0000 431 TABLE 39 Incidence of Microscopic Observations Unscheduled Deaths and Sacrifices 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS Covance 6329-183 3M T-6295 PAGE: 8 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C T E D -- SEX: ---MALE--- --FEMALE--- GROUP: -1 - -2 - -3- -4- -5- -6- -1 - -2 - -3- -4- -5- -6- ORGAN AND FINDING DESCRIPTION ---------------------------------------------------------- -- NUMBER: 39 40 33 25 29 30 26 35 41 35 25 23 -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ADRENAL, MEDULLA (MA) ................................. NUMBER EXAMINED: 39 40 33 25 29 30 26 35 41 35 25 23 NOT REMARKABLE: 34 28 22 21 23 26 23 25 37 27 21 21 --UNILATERALLY EXAMINED --HYPERPLASIA, MEDULLARY --B-PHEOCHROMOCYTOMA --M-MALIGNANT PHEOCHROMOCYTOMA 221 1 1 1 1 32420 1 5 81 32 02 02 02 255231 242220 1 2 000001 0000 LN, MESENTERIC (MS) ................................... NUMBER EXAMINED: 38 38 33 25 29 30 26 35 41 35 25 23 NOT REMARKABLE: 35 33 31 22 26 29 26 33 39 31 24 22 --HEMORRHAGE --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" --M-HEMANGIOSARCOMA --HYPERPLASIA, LYMPHOPLASMACYTIC --MINERALIZATION, VASCULAR --HYPERPLASIA, RETICULOENDOTHELIAL --HYPERPLASIA, LYMPHORETICULAR --INFILTRATE, EOSINOPHILIC --INFLAMMATION, GRANULOMATOUS --INFILTRATE, MACROPHAGE, PIGMENTED --B-HEMANGIOMA --INFLAMMATION, CHRONIC-ACTIVE --FIBROSIS --ANGIECTASIS FOR TYPE) 2 1 01 000001 1 1 01 001 00001 00 0001 00000000 000001 01 01 1 0 1 31 1 1 0000000 000000001 000 000000001 000 001 000000000 001 000000000 00000001 0000 0000000001 00 00001 0000000 00001 0000000 00001 0000000 432 TABLE 39 Incidence of Microscopic Observations Unscheduled Deaths and Sacrifices 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS Covance 6329-183 3M T-6295 PAGE: 9 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R - O F - A N I M A L S - A F F E C T E D -- SEX: ---MALE--- --FEMALE--- GROUP: -1 - -2 - -3- -4- -5- -6- -1 - -2 - -3- -4- -5- -6- NUMBER: 39 40 33 25 29 30 26 35 41 35 25 23 OVARY (OV) ........................ --CYST --HEMATOMA --B-GRANULOSA/THECA CELL TUMOR --HYPERPLASIA, GRANULOSA CELL --CORPORA LUTEA, PERSISTENT .... NUMBER EXAMINED: 0 0 0 0 0 0 26 35 41 35 25 23 NOT REMARKABLE: 0 0 0 0 0 0 22 31 34 29 20 17 00000034 7634 00000000001 0 0000001 00000 00000000001 1 000000000001 TESTIS (TE) ....................... ... NUMBER EXAMINED: 39 40 33 25 29 30 0 0 0 0 0 0 NOT REMARKABLE: 28 23 23 19 20 19 0 0 0 0 0 0 --ATROPHY/DEGENERATION --GRANULOMA, SPERM --UNILATERALLY EXAMINED --INFLAMMATION, VASCULAR --M-HEMANGIOSARCOMA --MINERALIZATION, VASCULAR --HYPERPLASIA, INTERSTITIAL CELL (LEYDIG CELL) --B-INTERSTITIAL CELL TUMOR --M-MALIGNANT INTERSTITIAL CELL TUMOR --INFILTRATE, LYMPHOHISTIOCYTIC 10 12 7 5 6 6 0 0 0 0 0 0 1 01 000000000 000001 000000 1 641 23000000 000001 000000 22 0000000000 02 1 01 0000000 1 0001 1 000000 0001 00000000 1 00000000000 BONE, FEMUR (FE) .................. ... NUMBER EXAMINED: 39 40 33 25 29 30 26 35 41 33 25 23 NOT REMARKABLE: 39 35 32 24 28 29 26 34 41 33 24 23 --X-HEMATOPOIETIC NEOPLASIA (SEE HN) --HYPERPLASIA, BONE 00001 0000000 01 01 00000000 433 TABLE 39 Incidence of Microscopic Observations Unscheduled Deaths and Sacrifices 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS Covance 6329-183 3M T-6295 PAGE: 10 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C T E D -- SEX: --MALE--- --FEMALE--- GROUP: -1 - -2 - -3- -4- -5- -6- -1 - -2 - -3- -4- -5- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 39 40 33 25 29 30 26 35 41 35 25 23 ** FROM PREVIOUS PAGE ** BONE, FEMUR (FE) ................... --OSTEODYSTROPHY, FIBROUS --HYPERPLASIA, CARTILAGE --N-SARCOMA NUMBER EXAMINED: 39 40 33 25 29 30 26 35 41 33 25 23 NOT REMARKABLE: 39 35 32 24 28 29 26 34 41 33 24 23 04 1 000000000 00000001 001 0 000001 000000 MARROW, FEMUR (FM) ................. NUMBER EXAMINED: 39 40 33 25 29 30 26 35 41 33 25 23 NOT REMARKABLE: 32 29 22 17 21 23 18 16 29 23 14 16 --HYPOCELLULAR, MARROW --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" --HYPERPLASIA, MYELOID, MARROW --HYPERPLASIA, ERYTHROID, MARROW --CONGESTION --N-FIBROSARCOMA --HYPERPLASIA, MEGAKARYOCYTIC, MARROW FOR TYPE) 242 31 1 032 1 4 0 32 001 0000001 4 4 6 4 5 4 7 12 8 7 5 5 02 3 1 1 3 1 4 1 2 2 2 000000001 01 0 1 00000000000 01 0000000001 MARROW, STERNUM (SE) ............... NUMBER EXAMINED: 39 40 33 24 29 30 26 35 41 35 24 23 NOT REMARKABLE: 30 29 20 19 18 23 17 21 27 24 14 16 --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" --HYPERPLASIA, MYELOID, MARROW --HYPOCELLULAR, MARROW --CONGESTION --HYPERPLASIA, ERYTHROID, MARROW --HYPERPLASIA, MEGAKARYOCYTIC, MARROW --N-CARCINOMA FOR TYPE) 22 001 0000000 7 5 8 4 6 5 8 10 12 9 5 5 1 2 1 01 0001 03 0 000000001 01 0 02 4 1 2 3 1 4 032 2 01 0000000001 00001 0000000 434 TABLE 39 Incidence of Microscopic Observations Unscheduled Deaths and Sacrifices 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS Covance 6329-183 3M T-6295 PAGE: 11 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C T E D -- SEX: ---MALE--- --FEMALE--- GROUP: -1 - -2 - -3- -4- -5- -6- -1 - -2 - -3- -4- -5- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 39 40 33 25 29 30 26 35 41 35 25 23 ---------------------------------------------------------- ----------------- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** MARROW, STERNUM (SE) .................................. NUMBER EXAMINED: 39 40 33 24 29 30 26 35 41 35 24 23 NOT REMARKABLE: 30 29 20 19 18 23 17 21 27 24 14 16 --MYELOFIBROSIS 000001 000000 BONE, STERNUM (SB) .................................... NUMBER EXAMINED: 39 40 33 25 29 30 26 35 41 35 24 23 NOT REMARKABLE: 37 37 32 24 28 29 26 35 41 35 24 23 --X-HEMATOPOIETIC NEOPLASIA (SEE HN) --HYPERPLASIA, CARTILAGINOUS --HYPERPLASIA, BONE --OSTEODYSTROPHY, FIBROUS --N-SARCOMA 00001 0000000 1 001 00000000 1 001 00000000 031 000000000 000001 000000 SPINAL CORD (SC) ...................................... NUMBER EXAMINED: 39 40 33 25 29 30 26 35 41 35 25 23 NOT REMARKABLE: 38 39 33 25 27 29 25 35 40 34 25 22 --HEMORRHAGE --AXONAL SWELLING --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" --NECROSIS / HEMORRHAGE, WHITE MATTER --DEMYELINATION --MINERALIZATION, DURA FOR TYPE) 00001 01 00001 000000001 000 1 00000000000 01 0001 000000 0000000001 00 00001 0000000 THYMUS (TH) ............................................ NUMBER EXAMINED: 39 35 30 23 27 27 25 34 39 31 23 23 NOT REMARKABLE: 6 3 1 2 2 1 5 5 4 5 1 4 --HEMORRHAGE 2 01 2 32 1 1 2 1 22 435 TABLE 39 Incidence of Microscopic Observations Unscheduled Deaths and Sacrifices 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS Covance 6329-183 3M T-6295 PAGE: 12 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C T E D -- SEX: ---MALE--- --FEMALE--- GROUP: -1 - -2 - -3- -4- -5- -6- -1 - -2 - -3- -4- -5- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 39 40 33 25 29 30 26 35 41 35 25 23 ** FROM PREVIOUS PAGE ** THYMUS (TH) ............................................ NUMBER EXAMINED: 39 35 30 23 27 27 25 34 39 31 23 23 NOT REMARKABLE: 6 3 1 2 2 1 5 5 4 5 1 4 --DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY) --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" --M-THYMIC CARCINOMA --CYST --INFLAMMATION, CHRONIC-ACTIVE --EDEMA --HYPERPLASIA, LYMPHORETICULAR --INFLAMMATION, GRANULOMATOUS --HYPERPLASIA, EPITHELIAL FOR TYPE) 33 31 29 21 25 26 17 29 31 24 20 18 02 00000001 00 000000001 000 00001 02 02 03 0 000001 000000 0000001 00000 000000001 000 001 000000000 0000000001 00 LUNG (LU) .............................................. NUMBER EXAMINED: 39 40 33 25 29 30 26 35 41 35 25 23 NOT REMARKABLE: 25 26 21 16 10 11 15 17 27 20 5 10 --CONGESTION --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" --MINERALIZATION, VASCULAR --N-CARCINOMA --HEMORRHAGE --INFILTRATE, MACROPHAGE, ALVEOLAR --INFILTRATE, LYMPHOHISTIOCYTIC --INFLAMMATION, GRANULOMATOUS --HYALINE MATERIAL, ALVEOLAR SEPTA --HYPERPLASIA, TYPE II PNEUMOCYTE --LEUKOCYTES, INCREASED, INTRAVASCULAR FOR TYPE) 2 003 1 01 2 02 1 0 2 3 001 1 001 1 01 874 2 87 04 7 64 5 00001 002 02 1 1 1 002 1 1 01 01 00 2 3 7 1 9 9 9 8 6 6 16 8 002 2 01 2 2 01 1 3 1 01 002 001 1 00 001 000000000 001 00001 01 00 01 0001 000000 436 TABLE 39 Incidence of Microscopic Observations Unscheduled Deaths and Sacrifices 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS Covance 6329-183 3M T-6295 PAGE: 13 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C T E D -- SEX: ---MALE--- --FEMALE--- GROUP: -1 - -2 - -3- -4- -5- -6- -1 - -2 - -3- -4- -5- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 39 40 33 25 29 30 26 35 41 35 25 23 ---------------------------------------------------------- ----------------- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** LUNG (LU) .............................................. NUMBER EXAMINED: 39 40 33 25 29 30 26 35 41 35 25 23 NOT REMARKABLE: 25 26 21 16 10 11 15 17 27 20 5 10 --MINERALIZATION, SEPTAL --INFLAMMATION, VASCULAR --EDEMA, ALVEOLAR --FIBROSIS --MINERALIZATION, ALVEOLAR LUMINA --INFLAMMATION, SUBACUTE --B-ADENOMA, BRONCHIOLAR-ALVEOLAR --N-SARCOMA --EMBOLISM, ADIPOSE TISSUE --INFILTRATE, MACROPHAGE, PIGMENTED 02 2 1 0001 001 0 00000001 001 0 1 0000000001 0 0001 00000000 0001 00000000 01 0000000000 00001 0000000 1 01 003 000000 00000001 0000 0000000001 00 HEART (HT) ............................................. NUMBER EXAMINED: 39 40 33 25 29 30 26 35 41 35 25 23 NOT REMARKABLE: 18 11 8 6 13 11 17 21 27 21 13 16 --CARDIOMYOPATHY --INFILTRATE, LYMPHOHISTIOCYTIC --FIBROSIS --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" --MINERALIZATION, VASCULAR --INFILTRATE, MACROPHAGE, PIGMENTED --MINERALIZATION --INFLAMMATION, VASCULAR --M-ATRIOCAVAL MESOTHELIOMA --METAPLASIA, OSSEOUS / CARTILAGINOUS --N-SARCOMA FOR TYPE) 16 27 25 18 16 19 2 001 00 1 001 00 1 1 0000 1 4221 1 2 1 001 0 1 3 001 1 000001 01 0000 1 0001 0 1 01 001 8 14 13 12 12 00000 00000 00000 0002 2 00001 00002 00000 00000 1 01 00 00000 7 0 0 0 0 0 0 1 0 0 0 437 TABLE 39 Incidence of Microscopic Observations Unscheduled Deaths and Sacrifices 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS Covance 6329-183 3M T-6295 PAGE: 14 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C T E D -- SEX: ---MALE--- --FEMALE--- GROUP: -1 - -2 - -3- -4- -5- -6- -1 - -2 - -3- -4- -5- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 39 40 33 25 29 30 26 35 41 35 25 23 ** FROM PREVIOUS PAGE ** HEART (HT) ......................... .................. NUMBER EXAMINED: 39 40 33 25 29 30 26 35 41 35 25 23 NOT REMARKABLE: 18 11 8 6 13 11 17 21 27 21 13 16 --INFLAMMATION, CHRONIC-ACTIVE 0000000001 00 MUSCLE, SKELETAL (SM) .............. .................. NUMBER EXAMINED: 39 40 33 25 29 30 26 35 41 35 25 23 NOT REMARKABLE: 37 35 31 23 21 27 26 33 40 34 24 23 --M-FIBROSARCOMA --INFLAMMATION, CHRONIC-ACTIVE --BACTERIAL COLONIES --ATROPHY --INFILTRATE, LYMPHOHISTIOCYTIC --X-HEMATOPOIETIC NEOPLASIA (SEE --M-SARCOMA, UNDIFFERENTIATED "HEMATO NEOPLASIA" FOR TYPE) 0001 00000000 0001 00000000 0001 00000000 1 4 1 1 63 01 01 1 0 01 1 02 001 0000 000000001 000 1 00000000000 NERVE, SCIATIC (SN) ................ .................. NUMBER EXAMINED: 39 39 33 25 28 30 25 35 41 35 25 23 NOT REMARKABLE: 38 37 33 24 26 29 25 35 41 34 25 23 --DEGENERATION 1 2 01 2 1 0001 00 TRACHEA (TR) ....................... .................. NUMBER EXAMINED: 39 40 33 25 29 30 26 35 41 35 25 23 NOT REMARKABLE: 39 40 33 24 29 30 26 34 41 35 25 23 --EXUDATE, LUMINAL --INFLAMMATION, CHRONIC-ACTIVE 0001 0001 00000001 0000 0000 438 TABLE 39 Incidence of Microscopic Observations Unscheduled Deaths and Sacrifices 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS Covance 6329-183 3M T-6295 PAGE: 15 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R - O F - A N I M A L S - A F F E C T E D -- SEX: ---MALE--- --FEMALE--- GROUP: -1 - -2 - -3- -4- -5- -6- -1 - -2 - -3- -4- -5- -6- NUMBER: 39 40 33 25 29 30 26 35 41 35 25 23 ESOPHAGUS (ES) ..................... NUMBER EXAMINED: 39 40 33 25 29 30 26 35 41 35 25 23 NOT REMARKABLE: 38 39 33 22 29 30 25 35 41 35 24 23 --HYPERKERATOSIS --EXUDATE, LUMINAL --DILATATION, LUMINAL --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 0001 001 0001 0 0002 00000000 1 00000000000 01 0000000000 THYROID (TY) ....................... NUMBER EXAMINED: 39 39 33 25 28 29 26 35 41 35 25 23 NOT REMARKABLE: 23 23 23 17 18 20 10 22 24 23 18 10 --HYPERPLASIA, C-CELL --M-CARCINOMA, FOLLICULAR CELL --DILATATION / CYST, FOLLICULAR --B-ADENOMA, C-CELL --B-ADENOMA, FOLLICULAR CELL --HYPERTROPHY, FOLLICULAR CELL --INFLAMMATION, VASCULAR --M-CARCINOMA, C-CELL --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 5 5 4 2 2 2 10 11 13 6 6 11 1 1 001 0000000 421 341 000000 4 4 3 04 2 6 34 7 1 2 3 32 2 04 0001 00 0001 1 0000000 0000001 00000 000001 000000 01 0000000000 PARATHYROID (PT) ................... NUMBER EXAMINED: 38 38 33 23 28 27 26 35 40 34 25 23 NOT REMARKABLE: 33 32 28 21 26 26 26 33 40 34 25 23 --HYPERPLASIA --B-ADENOMA 56522 1 01 00000001 0000 0000 439 TABLE 39 Incidence of Microscopic Observations Unscheduled Deaths and Sacrifices 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS Covance 6329-183 3M T-6295 PAGE: 16 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R - O F - A N I M A L S - A F F E C T E D -- SEX: --MALE--- --FEMALE--- GROUP: -1 - -2 - -3- -4- -5- -6- -1 - -2 - -3- -4- -5- -6- NUMBER: 39 40 33 25 29 30 26 35 41 35 25 23 PITUITARY (PI) ...................... NUMBER EXAMINED: 39 40 33 25 28 30 26 35 41 35 25 23 NOT REMARKABLE: 7 6 1 4 6 3 0 2 1 3 2 1 --B-ADENOMA --HYPERPLASIA --ANGIECTASIS --CYST --X-HEMATOPOIETIC NEOPLASIA (SEE --INFLAMMATION, CHRONIC-ACTIVE --NECROSIS --M-CARCINOMA --I-SARCOMA "HEMATO NEOPLASIA" FOR TYPE) 26 27 31 20 19 21 26 31 38 30 21 21 5 62 1 34 02 1 1 2 1 1 00002 01 1 000 01 1 01 3 02 0000 000000001 000 1 00000000000 1 00000000000 000000001 1 00 01 0000000000 HARDERIAN GLAND (HG) ................ NUMBER EXAMINED: 39 40 32 25 29 30 26 35 41 35 25 23 NOT REMARKABLE: 36 32 24 20 26 24 18 29 33 29 20 19 --INFILTRATE, LYMPHOHISTIOCYTIC --ATROPHY --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" --DYSPLASIA, EPITHELIAL, GLANDULAR --INFLAMMATION, VASCULAR --I-SARCOMA FOR TYPE) 376526868643 002 01 000001 1 00000000001 0 00001 0000000 000000001 000 01 0000000000 SALIV GL, MANDIB (SG) ............... NUMBER EXAMINED: 39 40 33 25 29 30 26 35 41 35 25 23 NOT REMARKABLE: 39 39 32 24 28 30 26 34 41 34 24 23 --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) --HYPERPLASIA, GLANDULAR 01 000000001 0 0001 00000000 440 TABLE 39 Incidence of Microscopic Observations Unscheduled Deaths and Sacrifices 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS Covance 6329-183 3M T-6295 PAGE: 17 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C T E D -- SEX: --MALE--- --FEMALE--- GROUP: -1 - -2 - -3- -4- -5- -6- -1 - -2 - -3- -4- -5- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 39 40 33 25 29 30 26 35 41 35 25 23 -- -- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** SALIV GL, MANDIB (SG) ................................. NUMBER EXAMINED: 39 40 33 25 29 30 26 35 41 35 25 23 NOT REMARKABLE: 39 39 32 24 28 30 26 34 41 34 24 23 --ATROPHY --INFILTRATE, LYMPHOHISTIOCYTIC --M-SCHWANNOMA --UNILATERALLY EXAMINED --I-SARCOMA 00001 0000000 0000000001 00 00000001 0000 00000001 0000 001 000000000 STOMACH, GL (ST) ...................................... NUMBER EXAMINED: 39 39 33 25 28 30 26 35 41 35 25 23 NOT REMARKABLE: 32 28 24 15 22 22 25 32 38 32 20 22 --EDEMA --INFLAMMATION, ACUTE --HEMORRHAGE --EROSION --INFLAMMATION, CHRONIC-ACTIVE --ULCERATION --HYPERPLASIA, EPITHELIAL, GLANDULAR --MINERALIZATION, GLANDULAR MUCOSA --FIBROSIS --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" --MINERALIZATION, VASCULAR FOR TYPE) 1 1 01 1 1 1 2 01 2 0 1 1 24 1 1 1 2 01 2 0 0001 00000000 2 02 1 4 2 0001 3 1 37 01 1 2 01 1 01 0 000002 000000 4 5532 3 001 01 0 053222 01 01 1 0 01 01 1 0000000 000000001 000 01 001 0000000 STOMACH, NONGL (SU) ................................... NUMBER EXAMINED: 39 40 33 25 29 30 26 35 41 35 25 23 NOT REMARKABLE: 29 34 28 19 24 21 24 31 35 32 22 20 --ACANTHOSIS / HYPERKERATOSIS 10 6 5 6 5 9 2 4 6 3 3 2 441 TABLE 39 Incidence of Microscopic Observations Unscheduled Deaths and Sacrifices 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS Covance 6329-183 3M T-6295 PAGE: 18 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C T E D -- SEX: ---MALE--- --FEMALE--- GROUP: -1- -2- -3- -4- -5- -6- -1- -2- -3- -4- -5- -6- ORGAN AND FINDING DESCRIPTION NUMBER: ------------------------------------ -------------------------------------- ** FROM PREVIOUS PAGE ** STOMACH, NONGL (SU) .............. .................... NUMBER EXAMINED: NOT REMARKABLE: --EDEMA --INFLAMMATION, --ULCERATION --INFLAMMATION, --FIBROSIS SUBACUTE CHRONIC-ACTIVE 39 -=- 39 29 10 4 90 40 -=- 40 34 00 560 33 -=- 33 28 00 3 50 25 -=- 25 19 00 360 29 -=- 29 24 210 50 30 -=- 2301 00 4 50 26 -=- 26 24 21000 35 -=- 35 31 00 4 40 41 -=- 41 35 221 30 35 -=- 35 32 210 31 25 -=- 2225 21100 23 -=- 2203 11000 DUODENUM (DU) .................... .................... NUMBER EXAMINED: 36 39 32 24 26 30 26 35 39 33 24 23 NOT REMARKABLE: 34 39 32 24 26 28 26 34 39 32 24 23 --INFLAMMATION, VASCULAR --INFLAMMATION, CHRONIC-ACTIVE --ULCERATION --EROSION --B-LEIOMYOMA 11100 00000 00000 00000 00000 11100 00000 10000 00000 10000 00000 00000 21 22 21JEJUNUM (JE) ..................... .................... NUMBER EXAMINED: 31 36 27 19 21 22 21NOT REMARKABLE: 31 36 27 19 26 25 33 35 32 26 25 33 35 32 ILEUM (IL) ......................... .................. NUMBER EXAMINED: 30 NOT REMARKABLE: 29 35 34 27 26 2211 2211 24 24 26 26 32 32 37 37 29 28 2211 2211 --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) --HEMORRHAGE --NECROSIS 110 100 000 000 000 000 000 000 000 100 000 000 442 TABLE 39 Incidence of Microscopic Observations Unscheduled Deaths and Sacrifices 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS Covance 6329-183 3M T-6295 PAGE: 19 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C T E D -- SEX: ---MALE--- --FEMALE--- GROUP: -1 - -2 - -3- -4- -5- -6- -1 - -2 - -3- -4- -5- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 39 40 33 25 29 30 26 35 41 35 25 23 ** FROM PREVIOUS PAGE ** ILEUM (IL) ......................... NUMBER EXAMINED: 30 35 27 21 21 24 26 32 37 29 21 21 NOT REMARKABLE: 29 34 26 21 21 24 26 32 37 28 21 21 --INFILTRATE, EOSINOPHILIC --INFLAMMATION, GRANULOMATOUS 001 001 000000000 000000000 CECUM (CE) ......................... NUMBER EXAMINED: 27 33 26 20 22 23 25 33 37 31 22 21 NOT REMARKABLE: 27 31 26 20 20 23 24 33 36 30 22 21 --EROSION --HEMORRHAGE --INFLAMMATION, CHRONIC-ACTIVE --X-HEMATOPOIETIC NEOPLASIA (SEE --BACTERIA --ULCERATION --B-LIPOMA "HEMATO NEOPLASIA" FOR TYPE) 02 001 0000000 01 002 0000000 02 001 01 01 000 0000000001 00 00001 0000000 00001 0000000 00001 0000000 COLON (CO) ......................... NUMBER EXAMINED: 34 37 29 23 22 27 26 34 40 33 23 23 NOT REMARKABLE: 34 36 29 23 22 25 26 34 40 31 23 23 --PARASITISM --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 01 0002 0001 0000000001 00 00 RECTUM (RE) ........................ NUMBER EXAMINED: 37 40 31 25 26 29 26 35 41 35 25 23 NOT REMARKABLE: 35 39 31 25 26 27 24 35 38 35 22 22 --PARASITISM --INFLAMMATION, CHRONIC-ACTIVE 1 1 0002 1 02 02 1 00000000001 0 443 TABLE 39 Incidence of Microscopic Observations Unscheduled Deaths and Sacrifices 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS Covance 6329-183 3M T-6295 PAGE: 20 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C T E D -- SEX: ---MALE--- --FEMALE--- GROUP: -1 - -2 - -3- -4- -5- -6- -1 - -2 - -3- -4- -5- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 39 40 33 25 29 30 26 35 41 35 25 23 ** FROM PREVIOUS PAGE ** RECTUM (RE) ............................................ NUMBER EXAMINED: 37 40 31 25 26 29 26 35 41 35 25 23 NOT REMARKABLE: 35 39 31 25 26 27 24 35 38 35 22 22 --ULCERATION --INFLAMMATION, VASCULAR --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) --NECROSIS 00000000001 0 0000001 00000 000000001 000 1 00000000000 SKIN (SK) .............................................. NUMBER EXAMINED: 39 40 33 25 29 30 26 35 41 35 25 23 NOT REMARKABLE: 32 28 25 20 20 24 22 34 37 32 22 21 --ACANTHOSIS/HYPERKERATOSIS --INFLAMMATION, CHRONIC-ACTIVE --ULCERATION --HEMORRHAGE --CYST, EPIDERMAL INCLUSION --BACTERIAL COLONIES --SUPERFICIAL CRUSTING --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" --B-KERATOACANTHOMA --M-FIBROSARCOMA --B-FIBROMA --FIBROSIS --B-ADENOMA, BASAL CELL/SQUAMOUS/SEBACEOUS --B-LIPOMA --M-CARCINOMA, SQUAMOUS CELL --B-RHABOMYOMA --B-PAPILLOMA, SQUAMOUS CELL FOR TYPE) 32 1 1 5 01 1 1 1 1 1 3 5 004 2 1 01 1 1 1 03 002 01 001 01 1 00000000000 1 41 000000000 1 0002 0000000 1 0001 0001 01 0 1 1 0001 001 001 1 23324 000000 2 1 2 1 1 01 01 01 0 02 1 01 0001 000 01 00001 00000 01 0000000000 01 00000002 00 1 000001 00000 000001 000000 01 00002 00000 444 TABLE 39 Incidence of Microscopic Observations Unscheduled Deaths and Sacrifices 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS Covance 6329-183 3M T-6295 PAGE: 21 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C T E D -- SEX: ---MALE--- --FEMALE--- GROUP: -1 - -2 - -3- -4- -5- -6- -1 - -2 - -3- -4- -5- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 39 40 33 25 29 30 26 35 41 35 25 23 ---------------------------------------------------- ---------------------- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** SKIN (SK) ........................................ .... NUMBER EXAMINED: 39 40 33 25 29 30 26 35 41 35 25 23 NOT REMARKABLE: 32 28 25 20 20 24 22 34 37 32 22 21 --INFLAMMATION, CHRONIC --ANGIECTASIS WITH THROMBOSIS AND ENDOTHELIAL PROLIFERATION 00000001 001 0 00001 0000000 MAMMARY, FEMALE (MF) ............................. .... NUMBER EXAMINED: 0 0 0 0 0 0 26 35 40 35 25 23 NOT REMARKABLE: 0 0 0 0 0 0 2 3 1 3 5 3 --M-CARCINOMA --CYST / GALACTOCELE --INFLAMMATION, CHRONIC-ACTIVE --B-FIBROADENOMA --HYPERPLASIA, GLANDULAR --B-ADENOMA --INFLAMMATION, CHRONIC --FIBROSIS 0 0 0 0 0 0 6 11 14 10 8 3 0 0 0 0 0 0 19 19 29 22 15 15 0000004 4 2 2 00 0 0 0 0 0 0 8 18 14 18 4 8 0 0 0 0 0 0 11 12 29 16 5 9 0000004 4 4 5 32 000000002 01 0 00000001 0000 URINARY BLADDER (UB) ............................. .... NUMBER EXAMINED: 38 38 32 23 26 30 26 35 41 35 25 23 NOT REMARKABLE: 34 33 23 16 21 27 24 33 40 35 24 22 --HEMORRHAGE --INFILTRATE, LYMPHOHISTIOCYTIC --PROTEINACEOUS MATERIAL, LUMINAL --HYPERPLASIA, TRANSITIONAL CELL --MINERALIZED MATERIAL, LUMEN --INFILTRATE, MACROPHAGE, PIGMENTED --INFLAMMATION, CHRONIC-ACTIVE 01 1 1 1 0000000 1 051 001 1 1 000 221 321 000000 1 1 2 2 32 02 001 0 1 00000000001 00002 0000000 1 332 1 2 00001 0 445 TABLE 39 Incidence of Microscopic Observations Unscheduled Deaths and Sacrifices 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS Covance 6329-183 3M T-6295 PAGE: 22 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C T E D -- SEX: ---MALE--- --FEMALE--- GROUP: -1 - -2 - -3- -4- -5- -6- -1 - -2 - -3- -4- -5- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 39 40 33 25 29 30 26 35 41 35 25 23 ---------------------------------------------------------- ----------------- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** URINARY BLADDER (UB) .................................. NUMBER EXAMINED: 38 38 32 23 26 30 26 35 41 35 25 23 NOT REMARKABLE: 34 33 23 16 21 27 24 33 40 35 24 22 --INFLAMMATION, VASCULAR 0000001 00000 UTERUS (UT) ............................................ NUMBER EXAMINED: 0 0 0 0 0 0 26 35 41 35 25 23 NOT REMARKABLE: 0 0 0 0 0 0 19 25 27 19 21 18 --DILATATION --B-POLYP, ENDOMETRIAL, STROMAL --CYST --INFLAMMATION, CHRONIC-ACTIVE --HYPERTROPHY, ENDOMETRIUM --B-LEIOMYOMA --HYPERPLASIA, CYSTIC, ENDOMETRIAL 0000004 4 6 72 2 0000002 1 1 3 00 000000002 000 0000001 22 001 000000000001 00000001 0000 0000002 64 72 2 CERVIX (CV) ............................................ NUMBER EXAMINED: 0 0 0 0 0 0 26 34 41 35 25 23 NOT REMARKABLE: 0 0 0 0 0 0 24 27 33 31 22 17 --HYPERPLASIA, MUCINOUS --X-HEMATOPOIETIC NEOPLASIA --HYPERPLASIA, STROMAL --M-SARCOMA, STROMAL --B-POLYP, STROMAL --EXUDATE, LUMINAL (SEE "HEMATO NEOPLASIA" FOR TYPE) 0000001 5442 5 0000001 00000 000000002 01 1 00000001 0000 00000001 1 000 00000001 1 000 446 TABLE 39 Incidence of Microscopic Observations Unscheduled Deaths and Sacrifices 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS Covance 6329-183 3M T-6295 PAGE: 23 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C T E D -- SEX: ---MALE--- --FEMALE--- GROUP: -1 - -2 - -3- -4- -5- -6- -1 - -2 - -3- -4- -5- -6- ORGAN AND FINDING DESCRIPTION -------------------------------------- -- NUMBER: 39 40 33 25 29 30 26 35 41 35 25 23 -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- VAGINA (VA) ........................ .................. NUMBER EXAMINED: 0 0 0 0 0 0 26 34 41 35 25 23 NOT REMARKABLE: 0 0 0 0 0 0 16 19 19 15 14 11 --LUMEN, EXUDATE --HYPERPLASIA, MUCINOUS --INFLAMMATION, CHRONIC --X-HEMATOPOIETIC NEOPLASIA (SEE --INFLAMMATION, CHRONIC-ACTIVE --B-POLYP, STROMAL "HEMATO NEOPLASIA" FOR TYPE) 0 0 0 0 0 0 6 13 10 12 94 0 0 0 0 0 0 4 9 14 12 5 8 0000000001 00 0000001 00000 000000002 000 000000001 000 PROSTATE (PR) ...................... .................. NUMBER EXAMINED: 39 40 33 25 29 30 0 0 0 0 0 0 NOT REMARKABLE: 13 4 2 6 5 6 0 0 0 0 0 0 --INFLAMMATION, CHRONIC-ACTIVE --INFILTRATE, LYMPHOHISTIOCYTIC --ATROPHY --HYPERPLASIA, EPITHELIAL 25 35 30 19 24 23 0 0 0 0 0 0 1 1 1 001 000000 00001 0000000 1 00000000000 SEMINAL VESICLES (SV) .............. .................. NUMBER EXAMINED: 38 40 33 25 29 30 0 0 0 0 0 0 NOT REMARKABLE: 32 28 20 15 22 22 0 0 0 0 0 0 --SECRETION, DECREASED --SECRETION, INCREASED --HYPERPLASIA, EPITHELIAL --INFLAMMATION, CHRONIC-ACTIVE --ATROPHY --INFILTRATE, LYMPHOHISTIOCYTIC --EDEMA 3241 22 000000 04 72 1 2 000000 01 0000000000 2221 1 1 000000 242632 000000 0001 00000000 000001 000000 447 TABLE 39 Incidence of Microscopic Observations Unscheduled Deaths and Sacrifices 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS Covance 6329-183 3M T-6295 PAGE: 24 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C T E D -- SEX: ---MALE--- --FEMALE--- GROUP: -1 - -2 - -3- -4- -5- -6- -1 - -2 - -3- -4- -5- -6- ORGAN AND FINDING DESCRIPTION ---------------------------------------------------------- -- NUMBER: 39 40 33 25 29 30 26 35 41 35 25 23 -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- EPIDIDYMIDES (EP) ..................................... NUMBER EXAMINED: 39 40 33 25 29 30 0 0 0 0 0 0 NOT REMARKABLE: 32 36 23 18 19 20 0 0 0 0 0 0 --IMMATURE SPERM FORMS --INFILTRATE, LYMPHOHISTIOCYTIC --HYPOSPERMIA --ATROPHY/DEGENERATION --UNILATERALLY EXAMINED --VACUOLATION, TUBULAR EPITHELIUM --GRANULOMA, SPERM --INFLAMMATION, CHRONIC-ACTIVE --INFILTRATE, EOSINOPHILIC --INFLAMMATION, VASCULAR --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 1 221 62 000000 1 031 32 000000 2 1 2 2 02 000000 0001 2 0000000 000002 000000 1 22 3 01 000000 1 00000000000 1 00000000000 001 000000000 1 00000000000 000001 000000 HEMATO NEOPLASIA (HN) ................................. NUMBER EXAMINED: 39 40 33 25 29 30 26 35 41 35 25 23 NOT REMARKABLE: 36 37 33 25 27 29 25 35 39 34 24 22 --M-LYMPHOMA --M-SARCOMA, HISTIOCYTIC 1 2 001 0001 000 2 1 001 1 1 01 1 1 1 CAUSE OF DEATH (CD) ................................... NUMBER EXAMINED: 39 40 33 25 29 30 26 35 41 35 25 23 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 0 0 --UNDETERMINED MICROSCOPICALLY --NEOPLASM, BRAIN --NEOPLASM, HEMATOPOIETIC --NEOPLASM, PITUITARY 82 3332 0001 1 2 22 1 1 000001 00 2 3 002 1 001 1 01 18 21 20 14 12 15 18 24 33 24 15 16 448 TABLE 39 Incidence of Microscopic Observations Unscheduled Deaths and Sacrifices 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS Covance 6329-183 3M T-6295 PAGE: 25 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---SEX: ------------ MALE------------ ----------- FEMALE GROUP: -1 - -2 - -3- -4- -5- -6- -1 - -2 - -3- -4- -5- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 39 40 33 25 29 30 26 35 41 35 25 23 ** FROM PREVIOUS PAGE ** CAUSE OF DEATH (CD) ................................... NUMBER EXAMINED: 39 40 33 25 29 30 26 35 41 35 25 23 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 0 0 --NEOPLASM, SKIN/MUSCLE/SUBCUTIS --INFLAMMATION / INFECTION --NEOPLASM, MAMMARY GLAND --NEOPLASM, ABDOMINAL CAVITY --HEMORRHAGE --NEPHROPATHY --GI TRACT, EROSION/ULCERATION/HEMORRHAGE/INFLAMMATION --POSSIBLE TRAUMATIC INJURY --INFECTION / NECROSIS / CALCULUS, URINARY TRACT --NEOPLASM, PANCREAS --NEOPLASM, THORACIC CAVITY --HEPATIC ANOMALY / OBSTRUCTIVE HEPATOPATHY --HEMANGIOSARCOMA --NEOPLASM, HEART --DEGENERATION/NECROSIS/HEMORRHAGE, SPINAL CORD/BRAIN --NEOPLASM, LIVER --VASCULITIS, SYSTEMIC --NEOPLASM, UT/CV/VA/OV --NEOPLASM, KIDNEY --NEOPLASM, SPLEEN --EMBOLISM, PULMONARY --NEOPLASM, ADRENAL 32 32 1 1 1 01 01 0 2 1 1 1 42 001 01 1 00001 058554 3 0001 2 0000000 0000000001 00 1 6 32 1 2 0002 1 0 1 00001 000000 1 01 000000000 1 001 1 1 000000 01 00001 0001 0 00001 1 000000 00000000001 0 000001 000000 01 0000000000 01 0000000000 001 000000000 0000001 00000 00000001 0000 000001 000000 000002 000000 00000001 0000 00001 001 0000 449 TABLE 39 Incidence of Microscopic Observations Unscheduled Deaths and Sacrifices 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS Covance 6329-183 3M T-6295 PAGE: 26 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---- SEX: ---MALE --FEMALE-- GROUP: -1 - -2 - -3- -4- -5- -6- -1 - -2 - -3- -4- -5- -6- NUMBER: 39 40 33 25 29 30 26 35 41 35 25 23 LN, MEDIASTINAL (ML) .................................. NUMBER EXAMINED: 0 2 2 0 3 2 2 0 2 1 1 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 0 0 --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" --HEMORRHAGE --HYPERPLASIA, LYMPHOPLASMACYTIC --INFILTRATE, MAST CELL --INFILTRATE, MACROPHAGE, PIGMENTED --INFLAMMATION, GRANULOMATOUS --INFILTRATE, EOSINOPHILIC --FIBROSIS --LYMPHANGIECTASIS FOR TYPE) 01 001 0001 000 01 2 02 2 2 001 1 0 000000001 01 0 01 0000000000 0000002 00000 001 000000000 001 000000000 001 000000000 000001 000000 LN, AXILLARY (AX) ..................................... NUMBER EXAMINED: 1 4 4 1 3 2 7 14 11 13 3 7 NOT REMARKABLE: 1 0 2 0 1 1 2 4 0 4 0 0 --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" --HEMORRHAGE --HYPERPLASIA, LYMPHOPLASMACYTIC --INFILTRATE, MACROPHAGE, PIGMENTED --HYPERPLASIA, RETICULOENDOTHELIAL --CONGESTION --FIBROPLASIA, PERIPHERAL FOR TYPE) 01 0000000000 01 1 1 2 01 2 1 1 02 0 3 1 1 1 0 5 8 10 8 2 5 01 0001 1 01 31 3 00000001 1 000 001 001 000000 000000000001 LN, INGUINAL (IN) ..................................... NUMBER EXAMINED: 0 1 0 0 0 0 1 6 1 2 1 1 NOT REMARKABLE: 0 0 0 0 0 0 0 3 1 0 1 0 --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 01 0000000000 450 TABLE 39 Incidence of Microscopic Observations Unscheduled Deaths and Sacrifices 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS Covance 6329-183 3M T-6295 PAGE: 27 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C T E D -- SEX: ---MALE --FEMALE--- GROUP: -1 - -2 - -3- -4- -5- -6- -1 - -2 - -3- -4- -5- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 39 40 33 25 29 30 26 35 41 35 25 23 ** FROM PREVIOUS PAGE ** LN, INGUINAL (IN) ..................................... NUMBER EXAMINED: 0 1 0 0 0 0 1 6 1 2 1 1 NOT REMARKABLE: 0 0 0 0 0 0 0 3 1 0 1 0 --HYPERPLASIA, LYMPHOPLASMACYTIC 0000001 3 02 01 LN, OTHER (LN) ........................................ NUMBER EXAMINED: 1 1 2 0 1 0 0 0 0 0 1 0 NOT REMARKABLE: 0 0 1 000000000 --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) --HEMORRHAGE --INFILTRATE, MACROPHAGE, PIGMENTED 1 1 0000000000 001 000000000 001 01 000001 0 LN, RENAL (RL) ........................................ NUMBER EXAMINED: 0 3 3 2 1 2 0 1 0 1 1 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 0 0 --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" --HYPERPLASIA, LYMPHOPLASMACYTIC --HEMORRHAGE --INFILTRATE, MACROPHAGE, PIGMENTED --DILATATION, SINUSOIDAL --N-SARCOMA --LYMPHANGIECTASIS FOR TYPE) 01 0000000000 02 2 2 01 00001 0 01 2 1 1 1 0001 00 001 1 0001 01 00 001 1 00000000 000001 000000 000001 000000 LN, MANDIBULAR (MN) ................................... NUMBER EXAMINED: 2 3 6 0 2 4 6 4 10 7 5 2 NOT REMARKABLE: 1 00000000000 --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) --HYPERPLASIA, LYMPHOPLASMACYTIC 01 0000000000 1 1 6 01 064 9752 451 TABLE 39 Incidence of Microscopic Observations Unscheduled Deaths and Sacrifices 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS Covance 6329-183 3M T-6295 PAGE: 28 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C T E D -- SEX: --MALE --FEMALE--- GROUP: -1 - -2 - -3- -4- -5- -6- -1 - -2 - -3- -4- -5- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 39 40 33 25 29 30 26 35 41 35 25 23 -- -- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** LN, MANDIBULAR (MN) .................... .............. NUMBER EXAMINED: 2 3 6 0 2 4 6 4 10 7 5 2 NOT REMARKABLE: 1 00000000000 --HEMORRHAGE --LYMPHANGIECTASIS --INFILTRATE, MACROPHAGE, PIGMENTED --CONGESTION --I-SARCOMA --ABSCESS --INFLAMMATION, CHRONIC-ACTIVE 00001 2 1 02 1 1 1 001 001 000000 000000001 001 01 2 002 000001 000001 000000 00000001 0000 000000000001 VERTEBRA/MARROW (VT) ................... .............. NUMBER EXAMINED: 0 1 0000000000 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 0 0 --X-HEMATOPOIETIC NEOPLASIA (SEE HN) 01 0000000000 MAMMARY, MALE (MM) ..................... .............. NUMBER EXAMINED: 1 3 4 3 3 2 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 1 00000000 --HYPERPLASIA, CYSTIC --SECRETORY MATERIAL IN LUMINA --GALACTOCELE --INFLAMMATION, CHRONIC-ACTIVE --B-FIBROADENOMA --M-CARCINOMA 001 1 00000000 0001 00000000 1 34 02 1 000000 1 1 0000000000 001 1 1 0000000 000002 000000 452 TABLE 39 Incidence of Microscopic Observations Unscheduled Deaths and Sacrifices 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS Covance 6329-183 3M T-6295 PAGE: 29 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C T E D -- SEX: ---MALE --FEMALE--- GROUP: -1 - -2 - -3- -4- -5- -6- -1 - -2 - -3- -4- -5- -6- ORGAN AND FINDING DESCRIPTION -- NUMBER: 39 40 33 25 29 30 26 35 41 35 25 23 ----------------- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- COAGULATING GL (CG) ................................... NUMBER EXAMINED: 1 001 00000000 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 0 0 --SECRETORY MATERIAL, INCREASED 1 001 00000000 TAIL (TI) .............................................. NUMBER EXAMINED: 2 0 4 1 0 0 1 1 1 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 0 0 --INFLAMMATION, CHRONIC-ACTIVE --ULCERATION --CYST, EPIDERMAL INCLUSION --ACANTHOSIS / HYPERKERATOSIS --INFLAMMATION, VASCULAR --M-NEUROFIBROSARCOMA 2 04 1 0001 1 000 001 000001 000 1 00000000000 1 02 1 0001 0000 0000001 00000 001 000000000 LN, LUMBAR/ILIAC (LM) ................................. NUMBER EXAMINED: 2 1 4 2 2 4 1 3 7 4 0 2 NOT REMARKABLE: 0 0 0 0 1 1 0 0 0 3 0 0 --LYMPHANGIECTASIS --HYPERPLASIA, LYMPHOPLASMACYTIC --HEMORRHAGE --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" --INFLAMMATION, SUBACUTE --INFILTRATE, MACROPHAGE, PIGMENTED --INFLAMMATION, CHRONIC-ACTIVE FOR TYPE) 002 1 1 2 001 000 2 1 3 1 1 2 1 3 5 002 01 1 1 1 1 002 1 01 000000001 000 001 000000000 000000001 000 000000000001 453 TABLE 39 Incidence of Microscopic Observations Unscheduled Deaths and Sacrifices 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS Covance 6329-183 3M T-6295 PAGE: 30 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R - O F - A N I M A L S - A F F E C T E D -- SEX: ---MALE --FEMALE--- GROUP: -1 - -2 - -3- -4- -5- -6- -1 - -2 - -3- -4- -5- -6- NUMBER: 39 40 33 25 29 30 26 35 41 35 25 23 CAVITY, ABDOM (PC) ............... .................... NUMBER EXAMINED: 0 0 1 2 2 3 0 0 0 0 1 1 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 0 0 --INFLAMMATION / NECROSIS, FAT --M-FIBROSARCOMA --INFLAMMATION, CHRONIC-ACTIVE --HEMATOMA --INFLAMMATION, VASCULAR --THROMBUS 001 2 02 000001 00001 0000000 00000000001 0 00001 0000000 000001 000000 000001 000000 AUDITORY SEB GL (AS) ............. .................... NUMBER EXAMINED: 0 1 2 0 0 0 0 0 1 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 0 0 --M-CARCINOMA --I-SARCOMA 01 1 001 000001 000 000000000 PREPUTIAL GLAND (PG) ............. .................... NUMBER EXAMINED: 0 1 1 0 1 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 0 0 --INFLAMMATION, CHRONIC-ACTIVE --ECTASIA, DUCTAL 01 1 001 01 0000000 000000000 FOOT/FOOTPAD (FP) ................ .................... NUMBER EXAMINED: 2 0 0 3 4 1 2 1 1 2 1 1 NOT REMARKABLE: 1 00000000000 --INFLAMMATION, CHRONIC-ACTIVE --ULCERATION --ACANTHOSIS / HYPERKERATOSIS 1 0034 1 2 1 1 2 1 1 1 002 2 1 1 1 1 2 00 0001 31 1 1 1 2 1 1 454 TABLE 39 Incidence of Microscopic Observations Unscheduled Deaths and Sacrifices 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS Covance 6329-183 3M T-6295 PAGE: 31 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C T E D -- SEX: ---MALE --FEMALE--- GROUP: -1- -2- -3- -4- -5- -6- -1- -2- -3- -4- -5- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 39 40 33 25 29 30 26 35 41 35 25 23 ** FROM PREVIOUS PAGE ** FOOT/FOOTPAD (FP) ..................... ............... NUMBER EXAMINED: NOT REMARKABLE: 21 00 00 30 40 10 20 10 10 20 10 10 --B-FIBROMA --FIBROSIS --BACTERIA 000 000 000 000 110 000 100 100 100 000 000 000 AORTA (AO) ............................. ............... NUMBER EXAMINED: NOT REMARKABLE: 00 10 31 20 10 11 00 00 00 10 00 00 --M-LIPOSARCOMA --MINERALIZATION 00 10 20 20 10 00 00 00 00 10 00 00 CALVARIUM/SKULL (CA) .................. ............... NUMBER EXAMINED: NOT REMARKABLE: 00 10 10 00 00 00 00 00 00 00 00 00 --I-SARCOMA 011000000000 LN, ANT MES/PANC (AP) ................. ............... NUMBER EXAMINED: NOT REMARKABLE: 00 10 10 00 00 10 10 10 10 00 10 00 --HEMORRHAGE --HYPERPLASIA, LYMPHOPLASMACYTIC --INFILTRATE, MACROPHAGE, PIGMENTED 000 100 110 000 000 100 110 111 110 000 110 000 455 TABLE 39 Incidence of Microscopic Observations Unscheduled Deaths and Sacrifices 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS Covance 6329-183 3M T-6295 PAGE: 32 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R - O F - A N I M A L S - A F F E C T E D -- SEX: ---MALE --FEMALE--- GROUP: -1 - -2 - -3- -4- -5- -6- -1 - -2 - -3- -4- -5- -6- NUMBER: 39 40 33 25 29 30 26 35 41 35 25 23 HEAD, CORONAL (HC) ............... .................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 1 00 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 0 0 --INFLAMMATION, ACUTE, EAR 0000000001 00 JOINT, OTHER (JT) ................ .................... NUMBER EXAMINED: 0 0 2 01 0000000 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 0 0 --INFLAMMATION, CHRONIC-ACTIVE --PROBABLE FRACTURE 002 01 0000000 001 000000000 CLITORAL GLAND (CL) .............. .................... NUMBER EXAMINED: 0 0 0 0 0 0 1 1 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 0 0 --ECTASIA, DUCTAL --INFLAMMATION, CHRONIC-ACTIVE --HYPERKERATOSIS 0000001 1 00000001 00000001 0000 0000 0000 URETER (UE) ...................... .................... NUMBER EXAMINED: 0 0 0 0 0 1 0001 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 1 00 00 --UNILATERALLY EXAMINED 000001 000000 ADIPOSE TISSUE (AT) .............. .................... NUMBER EXAMINED: 0 0 1 0001 1 NOT REMARKABLE: 0 0 0 0 0 0 0 1 0000 0000 --M-LIPOSARCOMA --INFILTRATE, EOSINOPHILIC 0000001 00000 001 000000000 456 TABLE 39 Incidence of Microscopic Observations Unscheduled Deaths and Sacrifices 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS Covance 6329-183 3M T-6295 PAGE: 33 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C T E D -- SEX: --MALE --FEMALE--- GROUP: -1 - -2 - -3- -4- -5- -6- -1 - -2 - -3- -4- -5- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 39 40 33 25 29 30 26 35 41 35 25 23 ** FROM PREVIOUS PAGE ** ADIPOSE TISSUE (AT) ................ .................. NUMBER EXAMINED: 0 0 1 0001 1 NOT REMARKABLE: 0 0 0 0 0 0 0 1 0000 0000 --INFLAMMATION, GRANULOMATOUS 001 000000000 DIAPHRAGM (DP) ..................... .................. NUMBER EXAMINED: 0 0 0 0 0 0 1 00000 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 0 0 --I-LIPOSARCOMA 0000001 00000 CAVITY, THORACIC (TA) .............. .................. NUMBER EXAMINED: 0 0 0 0 0 0 1 00000 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 0 0 --B-HIBERNOMA 0000001 00000 LN, TRACHEOBRON (TB) ............... .................. NUMBER EXAMINED: 0 0 0 0 0 1 001 000 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 0 0 --HYPERPLASIA, LYMPHOPLASMACYTIC --DILATATION, SINUSOIDAL --HEMORRHAGE --N-SARCOMA 000000001 000 000001 000000 000001 000000 000001 000000 LN, POPLITEAL (PP) ................. .................. NUMBER EXAMINED: 0 0 0 0 0 0 0 0 1 001 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 0 0 --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) --HYPERPLASIA, PLASMACYTIC 000000001 000 000000000001 457 TABLE 39 Incidence of Microscopic Observations Unscheduled Deaths and Sacrifices 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS Covance 6329-183 3M T-6295 PAGE: 34 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C T E D -- SEX: ---MALE --FEMALE--- GROUP: -1 - -2 - -3- -4- -5- -6- -1 - -2 - -3- -4- -5- -6- ORGAN AND FINDING DESCRIPTION ----------------------------------- -- NUMBER: 39 40 33 25 29 30 26 35 41 35 25 23 -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- MUSCLE, OTHER (MO) .............. ..................... NUMBER EXAMINED: 0 0 1 000000000 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 0 0 --INFLAMMATION, GRANULOMATOUS --INFILTRATE, EOSINOPHILIC 001 001 000000000 000000000 PERICARDIAL SAC (PD) ............ ..................... NUMBER EXAMINED: 0 0 1 001 000000 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 0 0 --M-SARCOMA, UNDIFFERENTIATED --N-SARCOMA 000001 000000 001 000000000 PINNA (PN) ...................... ..................... NUMBER EXAMINED: 0 0 0 0 0 1 000000 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 0 0 --M-SARCOMA 000001 000000 ARTERY, OTHER (AU) .............. ..................... NUMBER EXAMINED: 0 0 1 000000000 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 0 0 --MINERALIZATION 001 000000000 AORTIC ARCH (AA) ................ ..................... NUMBER EXAMINED: 0 0 1 000000000 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 0 0 --MINERALIZATION 001 000000000 458 TABLE 39 Incidence of Microscopic Observations Unscheduled Deaths and Sacrifices 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS Covance 6329-183 3M T-6295 PAGE: 35 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C T E D -- SEX: --MALE --FEMALE--- GROUP: -1 - -2 - -3- -4- -5- -6- -1 - -2 - -3- -4- -5- -6- ORGAN AND FINDING DESCRIPTION -------------------------------------- -- NUMBER: 39 40 33 25 29 30 26 35 41 35 25 23 -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- LN, ILEOCECAL (IC) ................. .................. NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 1 00 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 0 0 --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 0000000001 00 VAS DEFERENS (VS) .................. .................. NUMBER EXAMINED: 0 0 0 0 0 1 NOT REMARKABLE: 0 0 0 0 0 1 000000 000000 BILE DUCT (BD) ..................... .................. NUMBER EXAMINED: 0 0 1 000000000 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 0 0 --CYST --INFLAMMATION, CHRONIC --THROMBUS 001 001 001 000000000 000000000 000000000 TRIGEMINAL NERVE (TG) .............. .................. NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 0 0 ** END OF LIST ** 459 TABLE 40 Incidence of Microscopic Observations All Animals 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS Covance 6329-183 3M T-6295 PAGE: 1 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C T E D -- SEX: ---MALE--- --FEMALE--- GROUP: -1 - -2 - -3- -4- -5- -6- -1 - -2 - -3- -4- -5- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 70 60 60 60 70 40 70 60 60 60 70 40 ** TOP OF LIST ** EYE (EY) ................................... .......... NUMBER EXAMINED: 65 44 38 30 65 30 65 40 46 40 65 24 NOT REMARKABLE: 58 41 33 29 60 28 60 35 42 35 59 22 --DYSTROPHY, CORNEAL IN EARLY STAGES, CONSISTS PRIMARILY OF FOCAL MINERALIZATION --INFLAMMATION, ACUTE --I-NEUROFIBROSARCOMA --ATROPHY, RETINAL --DEGENERATION, LENTICULAR --INFLAMMATION, CHRONIC-ACTIVE --UNILATERALLY EXAMINED --ULCERATION, CORNEAL --DEGENERATION, RETINAL 61 31 4241 2331 1 0001 02 3 1 2 3 1 001 000000000 001 0000001 00 01 000001 0000 01 0000001 01 0 000000001 000 000000001 01 0 0000001 00000 BRAIN (BR) ................................. .......... NUMBER EXAMINED: 65 45 45 35 65 34 65 48 51 48 65 30 NOT REMARKABLE: 42 24 14 10 43 17 32 15 13 15 37 10 --DILATATION, VENTRICULAR --M-OLIGODENDROGLIOMA --COMPRESSION, VENTRAL --HEMORRHAGE --M-ASTROCYTOMA --DEGENERATION, FOCAL --MINERALIZATION --INFILTRATE, MACROPHAGE, PIGMENTED --THROMBUS --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" --M-GRANULAR CELL TUMOR FOR TYPE) 13 12 14 10 8 7 16 14 20 11 14 11 1 00000000000 20 15 26 23 18 16 30 33 38 28 27 19 2 01 1 1 04 1 1 1 01 2 001 000001 00 001 000000000 01 4 1 2 01 01 2 1 0 1 2 00001 1 02 01 00000001 0000 1 0001 0000000 01 0000000000 460 TABLE 40 Incidence of Microscopic Observations All Animals 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS Covance 6329-183 3M T-6295 PAGE: 2 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C T E D -- SEX: --MALE--- --FEMALE--- GROUP: -1 - -2 - -3- -4- -5- -6- -1 - -2 - -3- -4- -5- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 70 60 60 60 70 40 70 60 60 60 70 40 -- ----------------- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** BRAIN (BR) ............................................. NUMBER EXAMINED: 65 45 45 35 65 34 65 48 51 48 65 30 NOT REMARKABLE: 42 24 14 10 43 17 32 15 13 15 37 10 --I-CARCINOMA --M-MENINGEAL SARCOMA --M-NEUROFIBROSARCOMA 0000001 01 2 01 01 0000000000 001 000000000 LIVER (LI) ............................................. NUMBER EXAMINED: 65 55 55 55 65 40 65 55 55 55 65 40 NOT REMARKABLE: 2 0 0 0 0 1 6 1 1 1 0 0 --INFILTRATE, LYMPHOHISTIOCYTIC --HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED --GRANULAR CYTOPLASM, EOSINOPHILIC, CENTRILOBULAR --PIGMENT, HEPATOCELLULAR, CENTRILOBULAR --NECROSIS, INDIVIDUAL HEPATOCYTE --VACUOLATION, HEPATOCELLULAR, MIDZONAL / CENTRILOBULAR --VACUOLATION, HEPATOCELLULAR, PERIPORTAL --FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL --FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC --DEGENERATION, CYSTIC --NECROSIS, COAGULATIVE --CONGESTION --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) --HEMATOPOIESIS, EXTRAMEDULLARY --HYPERPLASIA, BILE DUCT --FIBROSIS, PERIBILIARY --HYPERTROPHY, HEPATOCELLULAR, PERIPORTAL 51 43 44 45 45 31 42 42 38 41 56 32 0 2 4 22 42 3 2 1 4 16 52 2 0 0 0 0 14 0 0 0 0 7 36 1 0 0 0 0 6 0 0 0 0 1 36 0 5 4 6 5 14 4 7 6 6 6 15 3 3 3 6 13 19 3 1 1 0 1 4 1 15 14 14 16 15 9 15 15 22 16 22 11 20 21 23 24 24 16 27 21 18 17 21 13 10 7 16 17 13 8 16 20 13 21 20 11 5 15 19 17 22 15 0 1 1 2 4 1 1 41 624324222 1 1 1 1 1 02 2 1 000 22 0031 001 1 01 4 3 8 3 7 5 10 17 15 8 7 5 20 20 25 24 25 21 23 25 19 17 30 17 8 7 11 7 14 4 9 13 4 9 10 4 4 1 7 2 2 5 12 10 9 4 3 7 461 TABLE 40 Incidence of Microscopic Observations All Animals 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS Covance 6329-183 3M T-6295 PAGE: 3 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---SEX: ------------ MALE------------ ----------- FEMALE GROUP: -1 - -2 - -3- -4- -5- -6- -1 - -2 - -3- -4- -5- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 70 60 60 60 70 40 70 60 60 60 70 40 ** FROM PREVIOUS PAGE ** LIVER (LI) ............................................. NUMBER EXAMINED: 65 55 55 55 65 40 65 55 55 55 65 40 NOT REMARKABLE: 2 0 0 0 0 1 6 1 1 1 0 0 --INFILTRATE, MACROPHAGE, PIGMENTED --VACUOLATION, HEPATOCELLULAR --DEGENERATION / NECROSIS, MIDZONAL --DEGENERATION / NECROSIS, CENTRILOBULAR --HYPERTROPHY, HEPATOCELLULAR --HEMORRHAGE --CYST, BILIARY --INFLAMMATION, SUBACUTE --GLYCOGEN, HEPATOCELLULAR, INCREASED --ANOMALY, SECONDARY TO DIAPHRAGMATIC HERNIA --ANGIECTASIS --LEUKOCYTOSIS, SINUSOIDAL --B-ADENOMA, HEPATOCELLULAR --INFLAMMATION, CHRONIC-ACTIVE --ECTASIA, BILE DUCT --STASIS, BILE --MITOSIS, HEPATOCELLULAR, INCREASED --LIPIDOSIS, SUBCAPSULAR (TENSION LIPIDOSIS) --N-SARCOMA --B-CHOLANGIOMA --N-CARCINOMA --KARYOMEGALY --M-CARCINOMA, HEPATOCELLULAR 6 5 3 4 5 2 2 3 5 6 23 7 62 31 7332 0332 00001 0000000 1 001 5 1 01 02 2 1 00001 0000000 001 00001 01 00 01 01 01 31 1 1 42 01 00002 0001 0 1 3 001 0000000 001 000000001 1 1 2 02 06 1 2 1 1 2 00001 001 0000 0331 7 001 1 1 52 0001 1 1 0001 1 0 00000000001 0 00000000001 0 00001 001 1 000 003 1 001 1 02 1 0 000001 000000 0000000001 00 0001 1 0000000 01 0000000000 00000000001 0 462 TABLE 40 Incidence of Microscopic Observations All Animals 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS Covance 6329-183 3M T-6295 PAGE: 4 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C T E D -- SEX: --MALE--- --FEMALE--- GROUP: -1 - -2 - -3- -4- -5- -6- -1 - -2 - -3- -4- -5- -6- ORGAN AND FINDING DESCRIPTION -- NUMBER: 70 60 60 60 70 40 70 60 60 60 70 40 -- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- LIVER-OIL RED O (LI0) ............................. .... NUMBER EXAMINED: 5 0 0 0 5 0 5 0 0 0 5 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 0 0 --VACUOLES, OIL RED O-POSITIVE, HEPATOCELLULAR 5 0005 05 0005 0 KIDNEY (KD) ....................................... ... NUMBER EXAMINED: 65 55 55 55 65 40 65 55 55 55 65 40 NOT REMARKABLE: 2 4 2 4 2 3 4 12 7 5 8 1 --NEPHROPATHY, CHRONIC PROGRESSIVE --INFILTRATE, LYMPHOHISTIOCYTIC --DILATATION, PELVIC --MINERALIZATION, PELVIC --REGENERATION, TUBULAR EPITHELIUM --MINERALIZATION, TUBULAR --HEMATOPOIESIS, EXTRAMEDULLARY --ATROPHY, TUBULAR --VACUOLATION, TUBULAR EPITHELIUM --N-CARCINOMA --PIGMENT, TUBULAR --KARYOMEGALY, TUBULAR CELL --HYALINE DROPLETS, TUBULAR EPITHELIUM --INFILTRATE, MACROPHAGE, PIGMENTED --HYPERPLASIA, TRANSITIONAL CELL --INFLAMMATION, SUPPURATIVE, PELVIC --INFLAMMATION, CHRONIC-ACTIVE, PELVIC --NECROSIS, PAPILLARY --ECTASIA, TUBULAR --CAST, PROTEINACEOUS 49 43 49 45 50 36 35 29 26 28 40 21 522 34 031 422 0 3 5 4 2 10 8 2 2 4 1 3 2 12 7 10 10 7 5 47 34 36 42 36 31 321 421 1 1 0000 2 0 0 1 4 1 14 8 11 7 19 6 01 000001 1 1 00 000000001 000 00002 01 1 02 1 0 000000000001 3 1 003 01 02 1 4 1 00000001 0000 51 01 4 0000000 2 01 0001 0001 0 1 2 3 5 1 3 6 10 8 7 5 5 1 2 3 3 02 1 3 52 2 2 1 3 3 1 1 1 01 002 1 00002 0000000 1 1 01 0000001 0 00000000001 0 463 TABLE 40 Incidence of Microscopic Observations All Animals 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS Covance 6329-183 3M T-6295 PAGE: 5 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C T E D -- SEX: ---MALE--- --FEMALE--- GROUP: -1 - -2 - -3- -4- -5- -6- -1 - -2 - -3- -4- -5- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 70 60 60 60 70 40 70 60 60 60 70 40 ---------------------------------------------------------- ----------------- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** KIDNEY (KD) ............................................ NUMBER EXAMINED: 65 55 55 55 65 40 65 55 55 55 65 40 NOT REMARKABLE: 2 4 2 4 2 3 4 12 7 5 8 1 --CYST --INFLAMMATION, CHRONIC-ACTIVE --FIBROSIS, CORTICAL --ANGIECTASIS --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" --BACTERIAL COLONIES --DEGENERATION, CYSTIC, UNILATERAL --INFILTRATE, FAT, INTERSTITIAL --N-SARCOMA --M-CARCINOMA, TUBULAR CELL --ULCERATION, TRANSITIONAL EPITHELIUM, PELVIC --M-LIPOSARCOMA --THROMBUS --B-LIPOMA --THROMBUS, GLOMERULAR --B-PAPILLOMA, TRANSITIONAL CELL --M-RENAL MESENCHYMAL TUMOR --DEGENERATION TUBULAR --INFARCT FOR TYPE) 03 02 02 1 1 1 000 1 001 1 01 00000 1 00000000000 000001 000000 1 00000000000 0001 00000000 0000000001 00 000000001 000 1 00000000000 1 000001 00000 000001 000000 000001 000000 001 2 1 00001 00 00001 01 01 000 00001 0000000 000001 000000 001 000000000 00001 01 00000 0000001 00000 SPLEEN (SP) ............................................ NUMBER EXAMINED: 65 45 38 30 65 31 65 40 46 41 65 23 NOT REMARKABLE: 9 7 6 8 14 6 2 0 1 0 4 0 --PIGMENT, INCREASED --HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED 39 29 23 16 30 14 48 23 35 28 51 14 24 16 15 9 27 13 39 23 16 16 23 15 464 TABLE 40 Incidence of Microscopic Observations All Animals 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS Covance 6329-183 3M T-6295 PAGE: 6 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C T E D -- SEX: ---MALE--- --FEMALE--- GROUP: -1 - -2 - -3- -4- -5- -6- -1 - -2 - -3- -4- -5- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 70 60 60 60 70 40 70 60 60 60 70 40 ---------------------------------------------------------- ----------------- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** SPLEEN (SP) ............................................ NUMBER EXAMINED: 65 45 38 30 65 31 65 40 46 41 65 23 NOT REMARKABLE: 9 7 6 8 14 6 2 0 1 0 4 0 --X-HEMATOPOIETIC NEOPLASIA (SEE --DEPLETION, LYMPHOCYTIC --FIBROSIS, CAPSULAR --CYST, SEROSAL --M-OSTEOSARCOMA --HYPERPLASIA, LYMPHORETICULAR --M-HEMANGIOSARCOMA --HEMORRHAGE --NECROSIS "HEMATO NEOPLASIA" FOR TYPE) 1 1 002 00001 00 00001 0001 001 1 00001 1 01 01 0 00000000001 0 000001 000000 0000000001 01 000001 000000 00000001 0000 00000001 0000 PANCREAS (PA) ......................................... NUMBER EXAMINED: 65 54 55 55 65 40 65 55 55 55 65 40 NOT REMARKABLE: 21 14 12 12 17 8 43 32 32 31 40 21 --ZYMOGEN GRANULES, DECREASED --INFILTRATE, LYMPHOHISTIOCYTIC --INFILTRATE, MACROPHAGE, PIGMENTED --HYPERPLASIA, ISLET CELLS --ATROPHY, ACINAR --NECROSIS / INFLAMMATION, ADIPOSE TISSUE --HYPERTROPHY / BASOPHILIA, ACINAR CELL --M-CARCINOMA, ISLET CELL --B-ADENOMA, ISLET CELL --B-ADENOMA, ACINAR CELL --VACUOLATION, FOCAL --INFILTRATION, FAT, INTERSTITIAL 11 15 11 14 16 8 3 11 14 2 2 1 4 1 1 3 02 24 17 18 13 19 13 1 2 1 32322221 0 778973235 0000001 00 2 3 01 331 52 1 22 5531 01 434441 221 01 0000000 00000001 0 11 16 20 14 12 6 10 7 4 9 12 10 331 111 000 243 000 1 01 01 0 02 0 000 000 967 465 TABLE 40 Incidence of Microscopic Observations All Animals 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS Covance 6329-183 3M T-6295 PAGE: 7 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C T E D -- SEX: ---MALE--- --FEMALE--- GROUP: -1 - -2 - -3- -4- -5- -6- -1 - -2 - -3- -4- -5- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 70 60 60 60 70 40 70 60 60 60 70 40 ---------------------------------------------------------- ----------------- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** PANCREAS (PA) ......................................... NUMBER EXAMINED: 65 54 55 55 65 40 65 55 55 55 65 40 NOT REMARKABLE: 21 14 12 12 17 8 43 32 32 31 40 21 --INFLAMMATION, VASCULAR --THROMBUS --X-HEMATOPOIETIC NEOPLASIA (SEE --INFILTRATE, EOSINOPHILIC --FIBROSIS --INFLAMMATION, GRANULOMATOUS --NECROSIS --ECTOPIC HEPATOCYTES --B-FIBROMA --INFLAMMATION, CHRONIC-ACTIVE "HEMATO NEOPLASIA" FOR TYPE) 2 1 44 1 4 01 1 001 1 00002 000000 000001 001 000 001 000000000 001 0000001 1 0 001 000000000 00001 0000000 0001 00000000 00000000001 0 000000001 01 0 ADRENAL, CORTEX (AC) .................................. NUMBER EXAMINED: 65 47 38 31 65 30 65 45 47 45 65 26 NOT REMARKABLE: 18 8 13 12 18 8 6 7 8 6 8 1 --VACUOLATION, FOCAL --UNILATERALLY EXAMINED --CONGESTION --ANGIECTASIS --HEMATOPOIESIS, EXTRAMEDULLARY --INFILTRATE, MACROPHAGE, PIGMENTED --HEMORRHAGE --HYPERTROPHY, CORTICAL CELL --VACUOLATION, DIFFUSE --DEGENERATION, CYSTIC / HEMORRHAGIC --HYPERPLASIA, CORTICAL 9 15 10 110 1 00 333 1 01 6 00 000 10 5 3 1 21 232 422 3 15 00 00 11 00 04 00 2 16 11 02 24 7 8 05 3 3 3 1 2 1 02 00 01 001 00 2 21 16 24 15 13 9 325451 0 05 01 0 11 0 01 1 02 00 7 26 17 11 16 27 12 0000000 0 25 16 9 17 20 10 1 443552 466 TABLE 40 Incidence of Microscopic Observations All Animals 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS Covance 6329-183 3M T-6295 PAGE: 8 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C T E D -- SEX: ---MALE--- --FEMALE--- GROUP: -1 - -2 - -3- -4- -5- -6- -1 - -2 - -3- -4- -5- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 70 60 60 60 70 40 70 60 60 60 70 40 ** FROM PREVIOUS PAGE ** ADRENAL, CORTEX (AC) ............... NUMBER EXAMINED: 65 47 38 31 65 30 65 45 47 45 65 26 NOT REMARKABLE: 18 8 13 12 18 8 6 7 8 6 8 1 --PIGMENT --MINERALIZATION --ATROPHY, CORTICAL --B-ADENOMA --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" --DEGENERATION / NECROSIS, UNILATERAL --NECROSIS, FOCAL --FIBROSIS --N-SARCOMA --M-CARCINOMA --CORTICAL TISSUE, EXTRACAPSULAR FOR TYPE) 23 26 16 15 26 14 36 28 28 25 40 16 1 00001 000000 01 1 1 1 001 01 00 3 01 2 1 01 1 1 22 0 1 0001 0000000 001 01 001 1 01 1 001 000000000 001 001 00001 0 000001 000000 00001 001 0001 01 0000000000 ADRENAL, MEDULLA (MA) .............. NUMBER EXAMINED: 65 47 38 31 65 30 65 45 47 45 65 24 NOT REMARKABLE: 57 33 27 26 52 26 54 33 42 33 54 22 --UNILATERALLY EXAMINED --HYPERPLASIA, MEDULLARY --B-PHEOCHROMOCYTOMA --M-MALIGNANT PHEOCHROMOCYTOMA 331 1 21 643760 3 5 81 62 1 2 02 1 2 365351 552350 1 2 001 001 0000 LN, MESENTERIC (MS) ................ NUMBER EXAMINED: 64 43 38 30 65 30 65 43 46 40 65 24 NOT REMARKABLE: 60 38 36 26 58 29 64 39 44 36 62 22 --HEMORRHAGE --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 31 01 01 2 002 01 1 01 00001 21 00 467 TABLE 40 Incidence of Microscopic Observations All Animals 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS Covance 6329-183 3M T-6295 PAGE: 9 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C T E D -- SEX: ---MALE--- --FEMALE--- GROUP: -1- -2- -3- -4- -5- -6- -1- -2- -3- -4- -5- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 70 60 60 60 70 40 70 60 60 60 70 40 ------------------------------------------ --------------------------------- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** LN, MESENTERIC (MS) ................... ............... NUMBER EXAMINED: 64 43 38 30 65 30 65 43 46 40 65 24 22NOT REMARKABLE: 60 38 36 26 58 29 64 39 44 36 62 --M-HEMANGIOSARCOMA --HYPERPLASIA, LYMPHOPLASMACYTIC --MINERALIZATION, VASCULAR --HYPERPLASIA, RETICULOENDOTHELIAL --HYPERPLASIA, LYMPHORETICULAR --INFILTRATE, EOSINOPHILIC --INFLAMMATION, GRANULOMATOUS --INFILTRATE, MACROPHAGE, PIGMENTED --B-HEMANGIOMA --INFLAMMATION, CHRONIC-ACTIVE --FIBROSIS --ANGIECTASIS --INFLAMMATION, VASCULAR --LYMPHANGIECTASIS 1000000000 00 30000000 1110000000 1110000000 1110000000 1000000000 1000000000 2100000000 1100000000 1100000000 1000000000 1000000000 0000 0000 0000 1000 1100 0000 0000 0000 0000 0000 1000 0000 0 0 0 0 0 0OVARY (OV) ............................. ............... NUMBER EXAMINED: 0 0 0 0 0 0NOT REMARKABLE: 65 42 48 46 65 31 51 36 40 34 47 19 --CYST --HEMATOMA --B-GRANULOSA/THECA CELL TUMOR --HYPERPLASIA, GRANULOSA CELL --CORPORA LUTEA, PERSISTENT --UNILATERALLY EXAMINED 000000 000000 000000 000000 000000 000000 1221000 600000 710000 1200000 1321600 800 310 468 TABLE 40 Incidence of Microscopic Observations All Animals 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS Covance 6329-183 3M T-6295 PAGE: 10 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---SEX: ------------ MALE------------ ----------- FEMALE GROUP: -1 - -2 - -3- -4- -5- -6- -1 - -2 - -3- -4- -5- -6NUMBER: 70 60 60 60 70 40 70 60 60 60 70 40 TESTIS (TE) ....................... --ATROPHY/DEGENERATION --GRANULOMA, SPERM --UNILATERALLY EXAMINED --INFLAMMATION, VASCULAR --M-HEMANGIOSARCOMA --MINERALIZATION, VASCULAR --HYPERPLASIA, INTERSTITIAL CELL (LEYDIG CELL) --B-INTERSTITIAL CELL TUMOR --M-MALIGNANT INTERSTITIAL CELL 1TUMOR --INFILTRATE, LYMPHOHISTIOCYTIC NUMBER EXAMINED: 65 55 55 55 65 39 0 0 0 0 0 0 NOT REMARKABLE: 49 32 38 41 49 27 0 0 0 0 0 0 15 16 12 9 11 6 0 0 0 0 0 0 2 01 000000000 000001 000000 1 85423000000 000001 000000 2 3 01 1 0000000 02 2 02 1 000000 1 1 221 1 000000 0001 00000000 1 01 01 0000000 BONE, FEMUR (FE) .................. NUMBER EXAMINED: 60 40 33 25 60 30 60 35 41 33 60 23 NOT REMARKABLE: 60 35 32 24 59 29 60 34 41 33 59 23 --X-HEMATOPOIETIC NEOPLASIA --HYPERPLASIA, BONE --OSTEODYSTROPHY, FIBROUS --HYPERPLASIA, CARTILAGE --N-SARCOMA (SEE HN) 00001 0000000 01 01 00000000 04 1 000000000 00000001 001 0 000001 000000 MARROW, FEMUR (FM) ................ NUMBER EXAMINED: 60 40 33 25 60 30 60 35 41 33 60 23 NOT REMARKABLE: 48 29 22 17 48 23 42 16 29 23 40 16 --HYPOCELLULAR, MARROW --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 742351 5321 70 32 001 0000001 469 TABLE 40 Incidence of Microscopic Observations All Animals 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS Covance 6329-183 3M T-6295 PAGE: 11 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C T E D -- SEX: ---MALE--- --FEMALE--- GROUP: -1 - -2 - -3- -4- -5- -6- -1 - -2 - -3- -4- -5- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 70 60 60 60 70 40 70 60 60 60 70 40 ---------------------------------------------------------- ----------------- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** MARROW, FEMUR (FM) .................................... NUMBER EXAMINED: 60 40 33 25 60 30 60 35 41 33 60 23 NOT REMARKABLE: 48 29 22 17 48 23 42 16 29 23 40 16 --HYPERPLASIA, MYELOID, MARROW --HYPERPLASIA, ERYTHROID, MARROW --CONGESTION --N-FIBROSARCOMA --HYPERPLASIA, MEGAKARYOCYTIC, MARROW 4 4 6 4 5 4 12 12 8 7 10 5 02 3 1 1 3 1 4 1 2 32 000000001 01 0 1 00000000000 01 0000000001 MARROW, STERNUM (SE) .................................. NUMBER EXAMINED: 60 40 33 24 60 30 60 35 41 35 59 23 NOT REMARKABLE: 50 29 20 19 49 23 46 21 27 24 44 16 --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" --HYPERPLASIA, MYELOID, MARROW --HYPOCELLULAR, MARROW --CONGESTION --HYPERPLASIA, ERYTHROID, MARROW --HYPERPLASIA, MEGAKARYOCYTIC, MARROW --N-CARCINOMA --MYELOFIBROSIS FOR TYPE) 22 001 0000000 7 5 8 4 6 5 13 10 12 9 9 5 22 1 01 0001 03 0 000000001 01 0 02 4 1 2 3 1 4 03 32 01 0000000001 00001 0000000 000001 000000 BONE, STERNUM (SB) .................................... NUMBER EXAMINED: 60 40 33 25 60 30 60 35 41 35 59 23 NOT REMARKABLE: 58 37 32 24 58 29 59 35 41 35 58 23 --X-HEMATOPOIETIC NEOPLASIA (SEE HN) --HYPERPLASIA, CARTILAGINOUS --HYPERPLASIA, BONE --OSTEODYSTROPHY, FIBROUS 00002 0000000 1 001 001 0001 0 1 001 00000000 031 000000000 470 TABLE 40 Incidence of Microscopic Observations All Animals 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS Covance 6329-183 3M T-6295 PAGE: 12 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C T E D -- SEX: ---MALE--- --FEMALE--- GROUP: -1 - -2 - -3- -4- -5- -6- -1 - -2 - -3- -4- -5- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 70 60 60 60 70 40 70 60 60 60 70 40 ** FROM PREVIOUS PAGE ** BONE, STERNUM (SB) ........................ --N-SARCOMA NUMBER EXAMINED: 60 40 33 25 60 30 60 35 41 35 59 23 NOT REMARKABLE: 58 37 32 24 58 29 59 35 41 35 58 23 000001 000000 SPINAL CORD (SC) .......................... NUMBER EXAMINED: 60 40 33 25 60 30 60 35 41 35 60 23 NOT REMARKABLE: 59 39 33 25 58 29 58 35 40 34 60 22 --HEMORRHAGE --AXONAL SWELLING --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" --NECROSIS / HEMORRHAGE, WHITE MATTER --DEMYELINATION --MINERALIZATION, DURA --DEGENERATION, AXONAL --INFILTRATE, LYMPHOHISTIOCYTIC FOR TYPE) 00001 01 00001 000000001 000 1 00000000000 01 0001 000000 0000000001 00 00001 0000000 0000001 00000 0000001 00000 THYMUS (TH) ................................ NUMBER EXAMINED: 55 35 30 24 54 27 57 34 39 31 57 25 NOT REMARKABLE: 13 3 1 2 5 1 16 5 4 5 12 4 --HEMORRHAGE --DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY) --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" --M-THYMIC CARCINOMA --CYST --INFLAMMATION, CHRONIC-ACTIVE --EDEMA FOR TYPE) 4 01 2 62 31 2 1 42 42 31 29 22 46 26 35 29 31 24 40 18 02 00000001 00 00001 0001 000 00001 02 02 04 1 000001 000000 0000001 00000 471 TABLE 40 Incidence of Microscopic Observations All Animals 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS Covance 6329-183 3M T-6295 PAGE: 13 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C T E D -- SEX: ---MALE--- --FEMALE--- GROUP: -1 - -2 - -3- -4- -5- -6- -1 - -2 - -3- -4- -5- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 70 60 60 60 70 40 70 60 60 60 70 40 ---------------------------------------------------------- ----------------- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** THYMUS (TH) ............................................ NUMBER EXAMINED: 55 35 30 24 54 27 57 34 39 31 57 25 NOT REMARKABLE: 13 3 1 2 5 1 16 5 4 5 12 4 --HYPERPLASIA, LYMPHORETICULAR --INFLAMMATION, GRANULOMATOUS --HYPERPLASIA, EPITHELIAL 0000001 01 001 001 000000000 0000000001 01 LUNG (LU) .............................................. NUMBER EXAMINED: 60 50 50 49 60 40 60 50 50 50 60 40 NOT REMARKABLE: 33 33 31 27 25 14 34 25 31 28 17 19 --CONGESTION --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" --MINERALIZATION, VASCULAR --N-CARCINOMA --HEMORRHAGE --INFILTRATE, MACROPHAGE, ALVEOLAR --INFILTRATE, LYMPHOHISTIOCYTIC --INFLAMMATION, GRANULOMATOUS --HYALINE MATERIAL, ALVEOLAR SEPTA --HYPERPLASIA, TYPE II PNEUMOCYTE --LEUKOCYTES, INCREASED, INTRAVASCULAR --MINERALIZATION, SEPTAL --INFLAMMATION, VASCULAR --EDEMA, ALVEOLAR --FIBROSIS --MINERALIZATION, ALVEOLAR LUMINA --INFLAMMATION, SUBACUTE --B-ADENOMA, BRONCHIOLAR-ALVEOLAR FOR TYPE) 3 003 1 01 2 02 1 0 2 3 002 1 001 1 01 15 8 9 12 18 10 4 5 8 11 11 7 0001 1 01 3 02 2 2 2 002 3 1 2 1 01 1 0 6 5 8 2 16 14 17 12 11 10 32 11 3 04 2 2 2 7 3 03 84 1 01 002 001 1 00 001 000000000 1 03 02 002 02 1 0 01 0001 000000 02 2 1 0001 001 0 00000001 001 0 1 0000000001 0 0001 00000000 0001 00000001 01 0000000000 00001 0000000 472 TABLE 40 Incidence of Microscopic Observations All Animals 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS Covance 6329-183 3M T-6295 PAGE: 14 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C T E D -- SEX: ---MALE--- --FEMALE--- GROUP: -1 - -2 - -3- -4- -5- -6- -1 - -2 - -3- -4- -5- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 70 60 60 60 70 40 70 60 60 60 70 40 ---------------------------------------------------------- ----------------- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** LUNG (LU) .............................................. NUMBER EXAMINED: 60 50 50 49 60 40 60 50 50 50 60 40 NOT REMARKABLE: 33 33 31 27 25 14 34 25 31 28 17 19 --N-SARCOMA --EMBOLISM, ADIPOSE TISSUE --INFILTRATE, MACROPHAGE, PIGMENTED --FIBROSIS, PLEURAL/SUBPLEURAL 1 01 1 03 000000 00000001 0000 0000000001 01 00000000001 0 HEART (HT) ............................................. NUMBER EXAMINED: 60 40 33 25 60 30 60 35 41 35 60 23 NOT REMARKABLE: 25 11 8 6 19 11 37 21 27 21 34 16 --CARDIOMYOPATHY --INFILTRATE, LYMPHOHISTIOCYTIC --FIBROSIS --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" --MINERALIZATION, VASCULAR --HEMORRHAGE --INFILTRATE, MACROPHAGE, PIGMENTED --MINERALIZATION --INFLAMMATION, VASCULAR --M-ATRIOCAVAL MESOTHELIOMA --METAPLASIA, OSSEOUS / CARTILAGINOUS --N-SARCOMA --INFLAMMATION, CHRONIC-ACTIVE --M-ENDOCARDIAL SCHWANNOMA FOR TYPE) 30 27 25 18 41 19 22 14 13 12 25 2 001 0000000 1 001 0000000 1 1 000000000 1 4 2 2 2 1 0002 2 00001 000000 2 1 001 000001 1 3 001 1 00002 000001 00000 01 000000000 1 0001 01 01 00 1 01 001 00000 0000000001 0 00000000001 7 0 0 0 0 0 0 0 1 0 0 0 0 0 473 TABLE 40 Incidence of Microscopic Observations All Animals 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS Covance 6329-183 3M T-6295 PAGE: 15 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R - O F - A N I M A L S - A F F E C T E D -- SEX: ---MALE--- --FEMALE--- GROUP: -1 - -2 - -3- -4- -5- -6- -1 - -2 - -3- -4- -5- -6- NUMBER: 70 60 60 60 70 40 70 60 60 60 70 40 MUSCLE, SKELETAL (SM) .............. NUMBER EXAMINED: 60 40 33 25 60 30 60 35 41 36 60 23 NOT REMARKABLE: 57 35 31 23 50 27 60 33 40 35 59 23 --M-FIBROSARCOMA --INFLAMMATION, CHRONIC-ACTIVE --BACTERIAL COLONIES --DEGENERATION --ATROPHY --INFILTRATE, LYMPHOHISTIOCYTIC --X-HEMATOPOIETIC NEOPLASIA (SEE --M-SARCOMA, UNDIFFERENTIATED "HEMATO NEOPLASIA" FOR TYPE) 0001 00000000 0001 00000000 0001 00000000 00001 0000000 1 4 1 1 73 01 01 1 0 01 1 02 001 0000 000000001 000 2 00000000000 NERVE, SCIATIC (SN) ................ NUMBER EXAMINED: 60 39 33 25 59 30 59 35 41 35 60 23 NOT REMARKABLE: 59 37 33 24 56 29 59 35 41 34 60 23 --DEGENERATION 1 2 01 31 0001 00 TRACHEA (TR) ....................... NUMBER EXAMINED: 60 40 33 25 60 30 60 35 41 35 60 23 NOT REMARKABLE: 60 40 33 24 59 30 60 34 41 35 60 23 --EXUDATE, LUMINAL --INFLAMMATION, CHRONIC-ACTIVE --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 0001 0001 0000 00000001 0000 00001 0000000 ESOPHAGUS (ES) ..................... --HYPERKERATOSIS NUMBER EXAMINED: 60 40 33 25 60 30 59 35 41 35 60 23 NOT REMARKABLE: 59 39 33 22 59 30 58 35 41 35 59 23 0001 001 0001 0 474 TABLE 40 Incidence of Microscopic Observations All Animals 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS Covance 6329-183 3M T-6295 PAGE: 16 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C T E D -- SEX: ---MALE--- --FEMALE--- GROUP: -1 - -2 - -3- -4- -5- -6- -1 - -2 - -3- -4- -5- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 70 60 60 60 70 40 70 60 60 60 70 40 ** FROM PREVIOUS PAGE ** ESOPHAGUS (ES) ..................... NUMBER EXAMINED: 60 40 33 25 60 30 59 35 41 35 60 23 NOT REMARKABLE: 59 39 33 22 59 30 58 35 41 35 59 23 --EXUDATE, LUMINAL --DILATATION, LUMINAL --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 0002 00000000 1 00000000000 01 001 0000000 THYROID (TY) ....................... NUMBER EXAMINED: 60 49 50 49 59 39 60 50 50 50 60 40 NOT REMARKABLE: 35 26 28 22 33 22 30 26 24 27 37 17 --HYPERPLASIA, C-CELL --M-CARCINOMA, FOLLICULAR CELL --DILATATION / CYST, FOLLICULAR --B-ADENOMA, C-CELL --B-ADENOMA, FOLLICULAR CELL --HYPERTROPHY, FOLLICULAR CELL --INFLAMMATION, VASCULAR --M-CARCINOMA, C-CELL --X-HEMATOPOIETIC NEOPLASIA (SEE --B-HIBERNOMA --COLLOID, DECREASED "HEMATO NEOPLASIA" FOR TYPE) 9 8 10 7 9 4 20 19 19 15 19 16 31 1 2 1 1 0001 00 633982 000000 5 6 6 9 7 4 12 6 6 8 5 6 3 54 4 4 9 0002 1 1 001 1 1 0000000 0000001 00000 001 001 01 0001 01 001 0000000 00000000001 0 000000001 000 PARATHYROID (PT) ................... NUMBER EXAMINED: 59 48 50 47 59 37 60 49 49 49 59 39 NOT REMARKABLE: 52 41 44 40 55 34 60 47 49 48 58 39 --HYPERPLASIA --B-ADENOMA 67564 3 01 01 00 1 01 1 0001 001 0 475 TABLE 40 Incidence of Microscopic Observations All Animals 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS Covance 6329-183 3M T-6295 PAGE: 17 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R - O F - A N I M A L S - A F F E C T E D -- SEX: --MALE--- --FEMALE--- GROUP: -1 - -2 - -3- -4- -5- -6- -1 - -2 - -3- -4- -5- -6- NUMBER: 70 60 60 60 70 40 70 60 60 60 70 40 PITUITARY (PI) ...................... NUMBER EXAMINED: 60 45 46 36 59 38 60 50 47 46 60 34 NOT REMARKABLE: 13 61 4 16 3621 3 11 1 --B-ADENOMA --HYPERPLASIA --ANGIECTASIS --CYST --X-HEMATOPOIETIC NEOPLASIA (SEE --INFLAMMATION, CHRONIC-ACTIVE --NECROSIS --M-CARCINOMA --I-SARCOMA "HEMATO NEOPLASIA" FOR TYPE) 37 29 42 31 37 28 46 41 42 37 40 30 10 9 6 3 10 5 11 8 3 5 12 4 2 00002 32 2 05 0 01 2 1 1 3 02 01 2 1 000000001 000 1 00000000000 1 00000000000 000000001 2 01 01 0000000000 HARDERIAN GLAND (HG) ................ NUMBER EXAMINED: 60 40 33 25 60 30 60 35 41 35 60 23 NOT REMARKABLE: 55 32 25 20 54 24 48 29 33 29 51 19 --INFILTRATE, LYMPHOHISTIOCYTIC --ATROPHY --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" --DYSPLASIA, EPITHELIAL, GLANDULAR --INFLAMMATION, VASCULAR --I-SARCOMA FOR TYPE) 5 7 6 5 5 6 12 6 8 6 8 3 002 01 000001 1 00000000001 0 00001 0000000 000000001 000 01 0000000000 SALIV GL, MANDIB (SG) ............... NUMBER EXAMINED: 60 40 33 25 60 30 60 35 41 35 60 23 NOT REMARKABLE: 60 39 32 24 59 30 60 34 41 34 59 23 --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) --HYPERPLASIA, GLANDULAR 01 000000001 0 0001 00000000 476 TABLE 40 Incidence of Microscopic Observations All Animals 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS Covance 6329-183 3M T-6295 PAGE: 18 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C T E D -- SEX: --MALE--- --FEMALE--- GROUP: -1 - -2 - -3- -4- -5- -6- -1 - -2 - -3- -4- -5- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 70 60 60 60 70 40 70 60 60 60 70 40 -- -- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** SALIV GL, MANDIB (SG) ................................. NUMBER EXAMINED: 60 40 33 25 60 30 60 35 41 35 60 23 NOT REMARKABLE: 60 39 32 24 59 30 60 34 41 34 59 23 --ATROPHY --INFILTRATE, LYMPHOHISTIOCYTIC --M-SCHWANNOMA --UNILATERALLY EXAMINED --I-SARCOMA 00001 0000000 0000000001 00 00000001 0000 00000001 0000 001 000000000 STOMACH, GL (ST) ...................................... NUMBER EXAMINED: 60 45 39 30 59 34 60 37 42 35 60 27 NOT REMARKABLE: 48 29 24 18 43 23 56 34 38 32 53 24 --EDEMA --INFLAMMATION, ACUTE --HEMORRHAGE --CONGESTION --EROSION --INFLAMMATION, CHRONIC-ACTIVE --ULCERATION --HYPERPLASIA, EPITHELIAL, GLANDULAR --MINERALIZATION, GLANDULAR MUCOSA --FIBROSIS --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" --MINERALIZATION, VASCULAR FOR TYPE) 1 2 01 1 1 22 01 2 0 22341 1 421 1 30 0001 00000000 00001 0000000 2 02 3 72 001 1 4 2 5 7 1 2 2 3 01 1 02 1 001 003 00001 0 8 9 11 386 001 01 0 0532 32 01 01 1 0 01 01 2 0000000 000000001 000 01 001 0000000 477 TABLE 40 Incidence of Microscopic Observations All Animals 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS Covance 6329-183 3M T-6295 PAGE: 19 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---SEX: ------------ MALE------------ ----------- FEMALE GROUP: -1 - -2 - -3- -4- -5- -6- -1 - -2 - -3- -4- -5- -6NUMBER: 70 60 60 60 70 40 70 60 60 60 70 40 STOMACH, NONGL (SU) ................................... NUMBER EXAMINED: 60 40 33 25 60 30 60 36 41 35 60 23 NOT REMARKABLE: 49 34 28 19 55 21 58 31 35 32 56 20 --ACANTHOSIS / HYPERKERATOSIS --EDEMA --INFLAMMATION, SUBACUTE --ULCERATION --INFLAMMATION, CHRONIC-ACTIVE --FIBROSIS 11 6 5 6 5 9 2 5 6 3 4 2 00001 0002 1 01 1 00000002 01 0 4533241 41 230 965655253321 0000000001 00 DUODENUM (DU) ......................................... NUMBER EXAMINED: 57 39 32 24 57 30 60 35 39 33 59 23 NOT REMARKABLE: 54 39 32 24 57 28 60 34 39 32 59 23 --INFLAMMATION, VASCULAR --INFLAMMATION, CHRONIC-ACTIVE --ULCERATION --EROSION --B-LEIOMYOMA 1 00001 0001 00 2 00001 000000 000001 000000 1 00000000000 00000001 0000 JEJUNUM (JE) .......................................... NUMBER EXAMINED: 52 36 27 19 52 26 59 33 35 32 57 22 NOT REMARKABLE: 52 36 27 19 52 26 59 33 35 32 57 21 --M-CARCINOMA 000000000001 ILEUM (IL) ............................................. NUMBER EXAMINED: 51 35 27 21 52 24 60 32 37 29 56 21 NOT REMARKABLE: 49 34 26 21 52 24 59 32 37 28 54 21 --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 01 00000001 00 478 TABLE 40 Incidence of Microscopic Observations All Animals 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS Covance 6329-183 3M T-6295 PAGE: 20 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C T E D -- SEX: ---MALE--- --FEMALE--- GROUP: -1 - -2 - -3- -4- -5- -6- -1 - -2 - -3- -4- -5- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 70 60 60 60 70 40 70 60 60 60 70 40 ** FROM PREVIOUS PAGE ** ILEUM (IL) ......................... .......... NUMBER EXAMINED: 51 35 27 21 52 24 60 32 37 29 56 21 NOT REMARKABLE: 49 34 26 21 52 24 59 32 37 28 54 21 --HEMORRHAGE --NECROSIS --INFILTRATE, EOSINOPHILIC --INFLAMMATION, GRANULOMATOUS --INFLAMMATION, CHRONIC-ACTIVE --HYPERPLASIA, GALT (GUT ASSOCIATED LYMPHOID TISSUE) 1 00000000000 1 00000000000 001 000000000 001 000000000 1 0000000001 0 0000001 0001 0 CECUM (CE) ......................... .......... NUMBER EXAMINED: 48 33 26 20 53 24 59 33 37 31 57 21 NOT REMARKABLE: 47 31 26 20 51 24 58 33 36 30 56 21 --EROSION --HEMORRHAGE --INFLAMMATION, CHRONIC-ACTIVE --X-HEMATOPOIETIC NEOPLASIA (SEE --BACTERIA --ULCERATION --B-LIPOMA "HEMATO NEOPLASIA" FOR TYPE) 02 001 0000000 01 002 0000000 1 2 001 01 01 01 0 0000000001 00 00001 0000000 00001 0000000 00001 0000000 COLON (CO) ......................... .......... NUMBER EXAMINED: 55 37 29 23 53 27 60 35 40 33 58 23 NOT REMARKABLE: 52 36 29 23 53 25 59 34 40 31 57 23 --PARASITISM --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) --INFLAMMATION, CHRONIC-ACTIVE --DIVERTICULUM 2 1 0002 0001 00 0000000001 00 1 0000000001 0 0000001 00000 479 TABLE 40 Incidence of Microscopic Observations All Animals 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS Covance 6329-183 3M T-6295 PAGE: 21 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C T E D -- SEX: ---MALE--- --FEMALE--- GROUP: -1 - -2 - -3- -4- -5- -6- -1 - -2 - -3- -4- -5- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 70 60 60 60 70 40 70 60 60 60 70 40 ** FROM PREVIOUS PAGE ** COLON (CO) ................................. --B-LEIOMYOMA NUMBER EXAMINED: 55 37 29 23 53 27 60 35 40 33 58 23 NOT REMARKABLE: 52 36 29 23 53 25 59 34 40 31 57 23 00000001 0000 RECTUM (RE) ................................ NUMBER EXAMINED: 58 40 31 25 57 29 59 35 41 35 60 23 NOT REMARKABLE: 54 39 31 25 54 27 57 35 38 35 52 22 --PARASITISM --INFLAMMATION, CHRONIC-ACTIVE --ULCERATION --INFLAMMATION, VASCULAR --X-HEMATOPOIETIC NEOPLASIA (SEE --NECROSIS "HEMATO NEOPLASIA" FOR TYPE) 2 1 0032 1 02 06 1 1 0000000002 0 00000000001 0 0000001 00000 000000001 000 1 00000000000 SKIN (SK) .................................. NUMBER EXAMINED: 60 43 37 31 60 33 60 36 41 35 60 25 NOT REMARKABLE: 52 28 25 20 44 24 53 34 37 32 53 21 --ACANTHOSIS/HYPERKERATOSIS --INFLAMMATION, CHRONIC-ACTIVE --ULCERATION --HEMORRHAGE --DEGENERATION, MUSCLE, SUBCUTANEOUS --CYST, EPIDERMAL INCLUSION --BACTERIAL COLONIES --SUPERFICIAL CRUSTING --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" --B-KERATOACANTHOMA FOR TYPE) 42 1 2 5 031 1 1 2 1 4 6 01 6331 1 1 22 03 01 2 03 001 1 1 1 000001 1 0000 0000001 00000 2 4 1 02 000001 0 1 0002 0000000 2 0002 0001 01 0 1 1 001 1 001 001 1 44 656 000001 480 TABLE 40 Incidence of Microscopic Observations All Animals 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS Covance 6329-183 3M T-6295 PAGE: 22 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C T E D -- SEX: ---MALE--- --FEMALE--- GROUP: -1- -2- -3- -4- -5- -6- -1- -2- -3- -4- -5- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 70 60 60 60 70 40 70 60 60 60 70 40 ---------------------------------------------------- ---------------------- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** SKIN (SK) ........................................ .... NUMBER EXAMINED: 60 43 37 31 60 33 60 36 41 35 60 25 20 21NOT REMARKABLE: 52 28 25 44 24 53 34 37 32 53 --M-FIBROSARCOMA --B-FIBROMA --FIBROSIS --B-ADENOMA, BASAL CELL/SQUAMOUS/SEBACEOUS --B-LIPOMA --M-CARCINOMA, SQUAMOUS CELL --B-RHABOMYOMA --B-PAPILLOMA, SQUAMOUS CELL --INFLAMMATION, CHRONIC --ANGIECTASIS WITH THROMBOSIS AND ENDOTHELIAL PROLIFERATION --EDEMA 2100000000 1 231111000 2 310000000 2110000000 1111100000 1100000000 2111100000 1100000000 1100000000 2000000000 1111000000 1000000000 000010000000 MAMMARY, FEMALE (MF) ............................. .... NUMBER EXAMINED: NOT REMARKABLE: 00 00 00 00 00 00 60 5 50 4 492 50 3 608 40 3 --M-CARCINOMA --CYST / GALACTOCELE --INFLAMMATION, CHRONIC-ACTIVE --B-FIBROADENOMA --HYPERPLASIA, GLANDULAR --SECRETORY MATERIAL --M-FIBROSARCOMA --B-ADENOMA --INFLAMMATION, CHRONIC --FIBROSIS 0000000000 0000000000 0000000000 0000000000 0000000000 0000000000 11 23056 3461 710 12 28 4 222761000 15 322 19 3500 250 11 282 24 2810 700 14 21810 30 51 410 2242 210500 400 481 TABLE 40 Incidence of Microscopic Observations All Animals 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS Covance 6329-183 3M T-6295 PAGE: 23 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C T E D -- SEX: ---MALE--- --FEMALE--- GROUP: -1 - -2 - -3- -4- -5- -6- -1 - -2 - -3- -4- -5- -6- ORGAN AND FINDING DESCRIPTION ------------------------------------------ -- NUMBER: 70 60 60 60 70 40 70 60 60 60 70 40 -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- URINARY BLADDER (UB) .................. ............... NUMBER EXAMINED: 59 48 49 48 57 40 60 50 50 50 59 40 NOT REMARKABLE: 51 38 36 33 45 36 57 44 49 50 55 37 --HEMORRHAGE --INFILTRATE, LYMPHOHISTIOCYTIC --PROTEINACEOUS MATERIAL, LUMINAL --HYPERPLASIA, TRANSITIONAL CELL --MINERALIZED MATERIAL, LUMEN --INFILTRATE, MACROPHAGE, PIGMENTED --INFLAMMATION, CHRONIC-ACTIVE --INFLAMMATION, VASCULAR --DIVERTICULUM 01 1 1 1 0000001 4 365 01 1 51 032 344781 000000 1 1 3 54 2 02 001 1 1 00000000001 00002 0000000 1 3331 2 00001 0 0000001 00000 0000001 00000 UTERUS (UT) ............................ ............... NUMBER EXAMINED: 0 0 0 0 0 0 60 39 44 41 60 29 NOT REMARKABLE: 0 0 0 0 0 0 43 25 28 19 41 19 --DILATATION --B-POLYP, ENDOMETRIAL, STROMAL --CYST --INFLAMMATION, CHRONIC-ACTIVE --HYPERTROPHY, ENDOMETRIUM --B-LEIOMYOMA --HYPERPLASIA, CYSTIC, ENDOMETRIAL --M-LEIOMYOSARCOMA --HEMORRHAGE --B-ADENOMA 000000856 974 0000002 2 1 3 3 1 000000002 000 0000001 22 001 000000000001 00000001 0000 0 0 0 0 0 0 8 8 6 10 10 5 000000000001 0000000001 00 0000000001 00 482 TABLE 40 Incidence of Microscopic Observations All Animals 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS Covance 6329-183 3M T-6295 PAGE: 24 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---- SEX: ---MALE--- --FEMALE-- GROUP: -1 - -2 - -3- -4- -5- -6- -1 - -2 - -3- -4- -5- -6- NUMBER: 70 60 60 60 70 40 70 60 60 60 70 40 CERVIX (CV) ........................ NUMBER EXAMINED: 0 0 0 0 0 0 60 34 43 37 60 24 NOT REMARKABLE: 0 0 0 0 0 0 54 27 33 31 53 17 --CYST, SQUAMOUS --HYPERPLASIA, MUCINOUS --X-HEMATOPOIETIC NEOPLASIA --HYPERPLASIA, STROMAL --M-SARCOMA, STROMAL --B-POLYP, STROMAL --EXUDATE, LUMINAL --HYPERPLASIA, CYSTIC --HEMORRHAGE (SEE "HEMATO NEOPLASIA" FOR TYPE) 0000001 00000 0000004 5 5 54 5 0000001 00000 000000003 01 1 00000001 002 0 00000001 1 001 00000001 1 000 0000000001 00 00000000001 0 VAGINA (VA) ........................ NUMBER EXAMINED: 0 0 0 0 0 0 60 34 42 36 59 23 NOT REMARKABLE: 0 0 0 0 0 0 35 19 19 15 36 11 --LUMEN, EXUDATE --HYPERPLASIA, MUCINOUS --INFLAMMATION, CHRONIC --X-HEMATOPOIETIC NEOPLASIA (SEE --INFLAMMATION, CHRONIC-ACTIVE --B-POLYP, STROMAL --I-CARCINOMA "HEMATO NEOPLASIA" FOR TYPE) 0 0 0 0 0 0 10 13 10 12 13 4 0 0 0 0 0 0 15 9 15 13 13 8 0000000001 00 0000001 00000 000000002 000 000000001 000 0000001 00000 PROSTATE (PR) ...................... NUMBER EXAMINED: 60 41 38 29 60 31 0 0 0 0 0 0 NOT REMARKABLE: 19 4 2 6 16 6 0 0 0 0 0 0 --INFLAMMATION, CHRONIC-ACTIVE 39 36 35 23 44 24 0 0 0 0 0 0 483 TABLE 40 Incidence of Microscopic Observations All Animals 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS Covance 6329-183 3M T-6295 PAGE: 25 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---SEX: ------------ MALE------------ ----------- FEMALE GROUP: -1 - -2 - -3- -4- -5- -6- -1 - -2 - -3- -4- -5- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 70 60 60 60 70 40 70 60 60 60 70 40 ** FROM PREVIOUS PAGE ** PROSTATE (PR) ......................................... NUMBER EXAMINED: 60 41 38 29 60 31 0 0 0 0 0 0 NOT REMARKABLE: 19 4 2 6 16 6 0 0 0 0 0 0 --INFILTRATE, LYMPHOHISTIOCYTIC --ATROPHY --HYPERPLASIA, EPITHELIAL 2 1 1 001 000000 00001 0000000 1 001 00000000 SEMINAL VESICLES (SV) ................................. NUMBER EXAMINED: 59 40 34 26 60 31 0 0 0 0 0 0 NOT REMARKABLE: 50 28 21 15 49 22 0 0 0 0 0 0 --SECRETION, DECREASED --SECRETION, INCREASED --HYPERPLASIA, EPITHELIAL --INFLAMMATION, CHRONIC-ACTIVE --ATROPHY --INFILTRATE, LYMPHOHISTIOCYTIC --EDEMA 5241 23000000 1 47222 000000 01 0000000000 222241 000000 242632 000000 0001 00000000 000001 000000 EPIDIDYMIDES (EP) ..................................... NUMBER EXAMINED: 60 40 33 25 60 30 0 0 0 0 0 0 NOT REMARKABLE: 50 36 23 18 45 20 0 0 0 0 0 0 --IMMATURE SPERM FORMS --INFILTRATE, LYMPHOHISTIOCYTIC --HYPOSPERMIA --ATROPHY/DEGENERATION --UNILATERALLY EXAMINED --VACUOLATION, TUBULAR EPITHELIUM --GRANULOMA, SPERM 1 221 72 000000 4 031 42 000000 21 2232 000000 0001 2 0000000 000002 000000 1 2231 1 000000 1 00000000000 484 TABLE 40 Incidence of Microscopic Observations All Animals 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS Covance 6329-183 3M T-6295 PAGE: 26 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C T E D -- SEX: ---MALE--- --FEMALE--- GROUP: -1 - -2 - -3- -4- -5- -6- -1 - -2 - -3- -4- -5- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 70 60 60 60 70 40 70 60 60 60 70 40 -------------------------------------- ------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** EPIDIDYMIDES (EP) .................. .................. NUMBER EXAMINED: 60 40 33 25 60 30 0 0 0 0 0 0 NOT REMARKABLE: 50 36 23 18 45 20 0 0 0 0 0 0 --INFLAMMATION, CHRONIC-ACTIVE --INFILTRATE, EOSINOPHILIC --INFLAMMATION, VASCULAR --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 1 00000000000 001 000000000 1 00000000000 000001 000000 HEMATO NEOPLASIA (HN) .............. .................. NUMBER EXAMINED: 60 40 33 25 60 30 60 35 41 35 60 23 NOT REMARKABLE: 57 37 33 25 56 29 59 35 39 34 59 22 --M-LYMPHOMA --M-SARCOMA, HISTIOCYTIC 1 2 002 0001 000 2 1 002 1 1 01 1 1 1 CAUSE OF DEATH (CD) ................ .................. NUMBER EXAMINED: 65 55 55 55 65 40 65 55 55 55 65 40 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 0 0 --SCHEDULED SACRIFICE --UNDETERMINED MICROSCOPICALLY --NEOPLASM, BRAIN --NEOPLASM, HEMATOPOIETIC --NEOPLASM, PITUITARY --NEOPLASM, SKIN/MUSCLE/SUBCUTIS --INFLAMMATION / INFECTION --NEOPLASM, MAMMARY GLAND --NEOPLASM, ABDOMINAL CAVITY --HEMORRHAGE --NEPHROPATHY 26 15 22 30 36 10 39 20 14 20 40 17 82 3332 0001 1 2 22 1 1 000001 00 2 3 002 1 001 1 01 18 21 20 14 12 15 18 24 33 24 15 16 32 32 1 1 1 01 01 0 2 1 1 1 42 001 01 1 00001 058554 3 0001 2 0000000 0000000001 00 1 6 32 1 2 0002 1 0 485 TABLE 40 Incidence of Microscopic Observations All Animals 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS Covance 6329-183 3M T-6295 PAGE: 27 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C T E D -- SEX: ---MALE --FEMALE--- GROUP: -1 - -2 - -3- -4- -5- -6- -1 - -2 - -3- -4- -5- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 70 60 60 60 70 40 70 60 60 60 70 40 ** FROM PREVIOUS PAGE ** CAUSE OF DEATH (CD) ................................... NUMBER EXAMINED: 65 55 55 55 65 40 65 55 55 55 65 40 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 0 0 --GI TRACT, EROSION/ULCERATION/HEMORRHAGE/INFLAMMATION --POSSIBLE TRAUMATIC INJURY --INFECTION / NECROSIS / CALCULUS, URINARY TRACT --NEOPLASM, PANCREAS --NEOPLASM, THORACIC CAVITY --HEPATIC ANOMALY / OBSTRUCTIVE HEPATOPATHY --HEMANGIOSARCOMA --NEOPLASM, HEART --DEGENERATION/NECROSIS/HEMORRHAGE, SPINAL CORD/BRAIN --NEOPLASM, LIVER --VASCULITIS, SYSTEMIC --NEOPLASM, UT/CV/VA/OV --NEOPLASM, KIDNEY --NEOPLASM, SPLEEN --EMBOLISM, PULMONARY --NEOPLASM, ADRENAL 1 00001 000000 1 01 000000000 1 001 1 1 000000 01 00001 0001 0 00001 1 000000 00000000001 0 000001 000000 01 0000000000 01 0000000000 001 000000000 0000001 00000 00000001 0000 000001 000000 000002 000000 00000001 0000 00001 001 0000 LN, MEDIASTINAL (ML) .................................. NUMBER EXAMINED: 0 2 2 2 3 4 3 0 2 1 3 1 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 0 0 --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" --HEMORRHAGE --HYPERPLASIA, LYMPHOPLASMACYTIC --INFILTRATE, MAST CELL --INFILTRATE, MACROPHAGE, PIGMENTED FOR TYPE) 01 001 0001 000 01 2 02 2 2 001 2 0 0001 01 1 01 02 1 01 0000000000 0000002 00000 486 TABLE 40 Incidence of Microscopic Observations All Animals 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS Covance 6329-183 3M T-6295 PAGE: 28 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---- SEX: ---MALE --FEMALE-- GROUP: -1 - -2 - -3- -4- -5- -6- -1 - -2 - -3- -4- -5- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 70 60 60 60 70 40 70 60 60 60 70 40 ** FROM PREVIOUS PAGE ** LN, MEDIASTINAL (ML) .................................. NUMBER EXAMINED: 0 2 2 2 3 4 3 0 2 1 3 1 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 0 0 --INFLAMMATION, GRANULOMATOUS --INFILTRATE, EOSINOPHILIC --FIBROSIS --LYMPHANGIECTASIS 001 000000000 001 000000000 001 000000000 0002 02 00001 0 LN, AXILLARY (AX) ..................................... NUMBER EXAMINED: 5 6 6 4 9 3 14 18 13 18 9 11 NOT REMARKABLE: 4 0 2 2 5 2 5 4 1 5 2 2 --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" --HEMORRHAGE --HYPERPLASIA, LYMPHOPLASMACYTIC --INFILTRATE, MACROPHAGE, PIGMENTED --HYPERPLASIA, RETICULOENDOTHELIAL --CONGESTION --FIBROPLASIA, PERIPHERAL FOR TYPE) 01 001 0000000 01 22 3 032 1 2 1 2 0 5 3 2 2 0 7 12 11 12 6 7 1 1 01 01 22 1 4 1 3 00000001 1 000 001 001 000000 000000000001 LN, INGUINAL (IN) ..................................... NUMBER EXAMINED: 0 2 0 0 0 0 5 7 1 2 2 1 NOT REMARKABLE: 0 1 0 0 0 0 2 3 1 0 1 0 --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" --HYPERPLASIA, LYMPHOPLASMACYTIC --INFILTRATE, MACROPHAGE, PIGMENTED --HEMORRHAGE --N-CARCINOMA FOR TYPE) 01 0000000000 0000001 4 02 01 0000001 0001 0 0000001 0001 0 0000001 00000 487 TABLE 40 Incidence of Microscopic Observations All Animals 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS Covance 6329-183 3M T-6295 PAGE: 29 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---- SEX: ---MALE --FEMALE-- GROUP: -1 - -2 - -3- -4- -5- -6- -1 - -2 - -3- -4- -5- -6- NUMBER: 70 60 60 60 70 40 70 60 60 60 70 40 LN, OTHER (LN) ........................................ NUMBER EXAMINED: 1 1 2 0 1 0 0 0 0 0 2 0 NOT REMARKABLE: 0 0 1 000000000 --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) --HEMORRHAGE --INFILTRATE, MACROPHAGE, PIGMENTED 1 1 0000000000 001 00000001 0 001 01 000002 0 LN, RENAL (RL) ........................................ NUMBER EXAMINED: 0 4 3 3 3 3 2 1 0 1 1 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 0 0 --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" --HYPERPLASIA, LYMPHOPLASMACYTIC --HEMORRHAGE --INFILTRATE, MACROPHAGE, PIGMENTED --DILATATION, SINUSOIDAL --N-SARCOMA --LYMPHANGIECTASIS FOR TYPE) 01 0000000000 02 2 3 01 1 0001 0 02 2 1 3 1 1 001 00 001 1 01 1 1 01 00 001 1 2 0000000 000001 000000 000002 000000 LN, MANDIBULAR (MN) ................................... NUMBER EXAMINED: 5 4 7 2 4 5 13 6 10 10 9 7 NOT REMARKABLE: 1 0000000002 0 --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" --HYPERPLASIA, LYMPHOPLASMACYTIC --HEMORRHAGE --LYMPHANGIECTASIS --INFILTRATE, MACROPHAGE, PIGMENTED --CONGESTION --I-SARCOMA FOR TYPE) 01 0000000000 3 2 7 1 3 1 11 5 9 9 6 5 2 001 3381 222 5 1 01 002 000000 00001 0001 001 01 2 002 000001 000001 000000 488 TABLE 40 Incidence of Microscopic Observations All Animals 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS Covance 6329-183 3M T-6295 PAGE: 30 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C T E D -- SEX: ---MALE --FEMALE--- GROUP: -1 - -2 - -3- -4- -5- -6- -1 - -2 - -3- -4- -5- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 70 60 60 60 70 40 70 60 60 60 70 40 ------------------------------------------- -------------------------------- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** LN, MANDIBULAR (MN) .................... .............. NUMBER EXAMINED: 5 4 7 2 4 5 13 6 10 10 9 7 NOT REMARKABLE: 1 0000000002 0 --ABSCESS --INFLAMMATION, CHRONIC-ACTIVE 00000001 0000 000000000001 VERTEBRA/MARROW (VT) ................... .............. NUMBER EXAMINED: 0 1 0000000000 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 0 0 --X-HEMATOPOIETIC NEOPLASIA (SEE HN) 01 0000000000 MAMMARY, MALE (MM) ..................... .............. NUMBER EXAMINED: 3 4 5 4 5 2 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 1 00000000 --HYPERPLASIA, CYSTIC --SECRETORY MATERIAL IN LUMINA --GALACTOCELE --INFLAMMATION, CHRONIC-ACTIVE --B-FIBROADENOMA --M-CARCINOMA --B-ADENOMA 001 1 00000000 0001 00000000 24 5 031 000000 31 0000000000 001 22 0000000 1 00002 000000 00001 0000000 COAGULATING GL (CG) .................... .............. NUMBER EXAMINED: 2 001 00000000 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 0 0 --SECRETORY MATERIAL, INCREASED --INFLAMMATION, CHRONIC-ACTIVE 1 001 00000000 1 00000000000 489 TABLE 40 Incidence of Microscopic Observations All Animals 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS Covance 6329-183 3M T-6295 PAGE: 31 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C T E D -- SEX: ---MALE --FEMALE--- GROUP: -1 - -2 - -3- -4- -5- -6- -1 - -2 - -3- -4- -5- -6- ORGAN AND FINDING DESCRIPTION -- NUMBER: 70 60 60 60 70 40 70 60 60 60 70 40 ----------------- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- TAIL (TI) .............................................. NUMBER EXAMINED: 5 0 4 2 0 2 1 1 1 1 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 0 0 --INFLAMMATION, CHRONIC-ACTIVE --ULCERATION --CYST, EPIDERMAL INCLUSION --ACANTHOSIS / HYPERKERATOSIS --INFLAMMATION, VASCULAR --M-NEUROFIBROSARCOMA --SUPERFICIAL CRUSTING --B-KERATOACANTHOMA --M-LIPOSARCOMA 2 04 1 02 01 1 1 00 001 000001 000 3 00000000000 2 02 1 02 01 0000 0000001 00000 001 000000000 000001 000000 0001 00000000 0000000001 00 LN, LUMBAR/ILIAC (LM) ................................. NUMBER EXAMINED: 4 1 5 6 2 6 2 4 9 8 7 6 NOT REMARKABLE: 0 0 0 0 1 1 0 0 0 5 1 1 --LYMPHANGIECTASIS --HYPERPLASIA, LYMPHOPLASMACYTIC --HEMORRHAGE --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" --INFLAMMATION, SUBACUTE --INFILTRATE, MACROPHAGE, PIGMENTED --INFLAMMATION, CHRONIC-ACTIVE --N-CARCINOMA FOR TYPE) 1 034 1 4 001 001 21 441 21 47254 1 1 1 1 1 1 002 1 2 2 000000001 000 001 000000000 1 00000001 1 00 000000000001 0000001 00000 490 TABLE 40 Incidence of Microscopic Observations All Animals 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS Covance 6329-183 3M T-6295 PAGE: 32 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R - O F - A N I M A L S - A F F E C T E D -- SEX: ---MALE --FEMALE--- GROUP: -1 - -2 - -3- -4- -5- -6- -1 - -2 - -3- -4- -5- -6- NUMBER: 70 60 60 60 70 40 70 60 60 60 70 40 CAVITY, ABDOM (PC) ............... .................... NUMBER EXAMINED: 1 0 1 3 2 4 1 1 0 0 4 1 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 0 0 --INFLAMMATION / NECROSIS, FAT --M-FIBROSARCOMA --INFLAMMATION, CHRONIC-ACTIVE --HEMATOMA --INFLAMMATION, VASCULAR --THROMBUS --M-LIPOSARCOMA 1 01 2 02 01 003 1 00001 0000000 1 0000000001 0 00001 0000000 0001 02 000000 0001 01 000000 0000001 00000 AUDITORY SEB GL (AS) ............. .................... NUMBER EXAMINED: 0 1 2 0 0 0 0 0 1 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 0 0 --M-CARCINOMA --I-SARCOMA 01 1 001 000001 000 000000000 PREPUTIAL GLAND (PG) ............. .................... NUMBER EXAMINED: 0 1 2 0 1 2 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 0 0 --INFLAMMATION, CHRONIC-ACTIVE --ECTASIA, DUCTAL 01 2 01 2 000000 002 001 000000 FOOT/FOOTPAD (FP) ................ .................... NUMBER EXAMINED: 2 2 3 6 4 2 2 3 2 2 5 2 NOT REMARKABLE: 1 00000000000 --INFLAMMATION, CHRONIC-ACTIVE --ULCERATION 1 23642232252 1 23421 1 22221 491 TABLE 40 Incidence of Microscopic Observations All Animals 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS Covance 6329-183 3M T-6295 PAGE: 33 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C T E D -- SEX: --MALE --FEMALE--- GROUP: -1 - -2 - -3- -4- -5- -6- -1 - -2 - -3- -4- -5- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 70 60 60 60 70 40 70 60 60 60 70 40 ** FROM PREVIOUS PAGE ** FOOT/FOOTPAD (FP) ..................... ............... NUMBER EXAMINED: 2 2 3 6 4 2 2 3 2 2 5 2 NOT REMARKABLE: 1 00000000000 --ACANTHOSIS / HYPERKERATOSIS --B-FIBROMA --FIBROSIS --BACTERIA --HYPERPLASIA, BONE 01 34 31 1 32242 0000001 00000 00001 1 01 1 01 1 01 01 1 001 001 0 000001 00001 0 AORTA (AO) ............................. ............... NUMBER EXAMINED: 0 1 3 NOT REMARKABLE: 0 0 1 221 001 0001 00 000000 --M-LIPOSARCOMA --MINERALIZATION 00001 01 22 1 0000000 00001 00 CALVARIUM/SKULL (CA) .................. ............... NUMBER EXAMINED: 0 1 1 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 0 0 --I-SARCOMA 01 1 000000000 LN, ANT MES/PANC (AP) ................. ............... NUMBER EXAMINED: 0 1 2 0 1 2 1 1 1 0 1 0 NOT REMARKABLE: 0 0 0 0 1 1 0 0 0 0 0 0 --HEMORRHAGE --HYPERPLASIA, LYMPHOPLASMACYTIC --INFILTRATE, MACROPHAGE, PIGMENTED 01 1 0001 1 1 01 0 002 0001 1 001 0 002 001 01 1 000 492 TABLE 40 Incidence of Microscopic Observations All Animals 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS Covance 6329-183 3M T-6295 PAGE: 34 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C T E D -- SEX: ---MALE --FEMALE--- GROUP: -1 - -2 - -3- -4- -5- -6- -1 - -2 - -3- -4- -5- -6- ORGAN AND FINDING DESCRIPTION ------------------------------------ -- NUMBER: 70 60 60 60 70 40 70 60 60 60 70 40 -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- HEAD, CORONAL (HC) ............... .................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 1 00 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 0 0 --INFLAMMATION, ACUTE, EAR 0000000001 00 JOINT, OTHER (JT) ................ .................... NUMBER EXAMINED: 1 1 3 1 1 3 0 0 0 0 1 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 0 0 --INFLAMMATION, CHRONIC-ACTIVE --PROBABLE FRACTURE --FIBROSIS --HYPERPLASIA, BONE 1 1 31 1 3 00001 0 001 000000000 1 00001 000000 000001 000000 CLITORAL GLAND (CL) .............. .................... NUMBER EXAMINED: 0 0 0 0 0 0 1 1 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 0 0 --ECTASIA, DUCTAL --INFLAMMATION, CHRONIC-ACTIVE --HYPERKERATOSIS 0000001 1 00000001 00000001 0000 0000 0000 URETER (UE) ...................... .................... NUMBER EXAMINED: 0 0 0 0 0 1 0001 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 1 00 00 --UNILATERALLY EXAMINED 000001 000000 493 TABLE 40 Incidence of Microscopic Observations All Animals 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS Covance 6329-183 3M T-6295 PAGE: 35 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C T E D -- SEX: ---MALE --FEMALE--- GROUP: -1- -2- -3- -4- -5- -6- -1- -2- -3- -4- -5- -6- ORGAN AND FINDING DESCRIPTION -- NUMBER: 70 60 60 60 70 40 70 60 60 60 70 40 ------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ADIPOSE TISSUE (AT) ................ .................. NUMBER EXAMINED: NOT REMARKABLE: 00 00 10 00 00 00 10 11 00 00 00 00 --M-LIPOSARCOMA --INFILTRATE, EOSINOPHILIC --INFLAMMATION, GRANULOMATOUS 000 000 110 000 000 000 100 000 000 000 000 000 DIAPHRAGM (DP) ..................... .................. NUMBER EXAMINED: NOT REMARKABLE: 00 00 00 00 00 00 10 00 00 00 00 00 --I-LIPOSARCOMA 000000100000 CAVITY, THORACIC (TA) .............. .................. NUMBER EXAMINED: NOT REMARKABLE: 10 00 00 00 00 00 10 00 00 00 00 00 --B-HIBERNOMA --B-AORTIC BODY TUMOR 10 00 00 00 00 00 10 00 00 00 00 00 LN, TRACHEOBRON (TB) ............... .................. NUMBER EXAMINED: NOT REMARKABLE: 00 00 00 00 00 10 00 00 10 00 00 00 --HYPERPLASIA, LYMPHOPLASMACYTIC --DILATATION, SINUSOIDAL --HEMORRHAGE --N-SARCOMA 0000 0000 0000 0000 0000 1110 0000 0000 1000 0000 0000 0000 494 TABLE 40 Incidence of Microscopic Observations All Animals 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS Covance 6329-183 3M T-6295 PAGE: 36 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C T E D -- SEX: ---MALE --FEMALE--- GROUP: -1 - -2 - -3- -4- -5- -6- -1 - -2 - -3- -4- -5- -6- ORGAN AND FINDING DESCRIPTION -- NUMBER: 70 60 60 60 70 40 70 60 60 60 70 40 ------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- LN, POPLITEAL (PP) ................. .................. NUMBER EXAMINED: 0 0 0 0 0 1 001 001 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 0 0 --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) --HYPERPLASIA, PLASMACYTIC 000000001 000 000001 000001 MUSCLE, OTHER (MO) ................. .................. NUMBER EXAMINED: 0 0 1 000000000 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 0 0 --INFLAMMATION, GRANULOMATOUS --INFILTRATE, EOSINOPHILIC 001 001 000000000 000000000 PERICARDIAL SAC (PD) ............... .................. NUMBER EXAMINED: 0 0 1 001 000000 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 0 0 --M-SARCOMA, UNDIFFERENTIATED --N-SARCOMA 000001 000000 001 000000000 PINNA (PN) ......................... .................. NUMBER EXAMINED: 0 0 0 0 0 1 000001 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 0 0 --M-SARCOMA --B-PAPILLOMA, SQUAMOUS CELL 000001 000000 000000000001 ARTERY, OTHER (AU) ................. .................. NUMBER EXAMINED: 0 0 1 000000000 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 0 0 --MINERALIZATION 001 000000000 495 TABLE 40 Incidence of Microscopic Observations All Animals 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS Covance 6329-183 3M T-6295 PAGE: 37 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C T E D -- SEX: --MALE --FEMALE--- GROUP: -1 - -2 - -3- -4- -5- -6- -1 - -2 - -3- -4- -5- -6- ORGAN AND FINDING DESCRIPTION -- -- NUMBER: 70 60 60 60 70 40 70 60 60 60 70 40 -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- AORTIC ARCH (AA) ................... .................. NUMBER EXAMINED: 0 0 1 000000000 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 0 0 --MINERALIZATION 001 000000000 LN, ILEOCECAL (IC) ................. .................. NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 1 00 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 0 0 --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 0000000001 00 VAS DEFERENS (VS) .................. .................. NUMBER EXAMINED: 0 0 0 0 0 1 NOT REMARKABLE: 0 0 0 0 0 1 000000 000000 BILE DUCT (BD) ..................... .................. NUMBER EXAMINED: 0 0 1 000000000 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 0 0 --CYST --INFLAMMATION, CHRONIC --THROMBUS 001 001 001 000000000 000000000 000000000 TRIGEMINAL NERVE (TG) .............. .................. NUMBER EXAMINED: 0 0 0 1 00000000 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 0 0 --GANGLION ** END OF LIST ** 0001 00000000 496 TABLE 41 Incidence of Microscopic Observations Recovery Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 1 TABLE INCLUDES: SEX=ALL;GROUP=6 ;WEEKS=ALL DEATH=U;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---SEX: MALE FEMALE GROUP: -6- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 10 17 ** TOP OF LIST ** EYE (EY) ......................................... ..... NUMBER EXAMINED: 0 1 NOT REMARKABLE: 0 0 --DYSTROPHY, CORNEAL IN EARLY STAGES, CONSISTS PRIMARILY OF FOCAL MINERALIZATION 01 BRAIN (BR) ........................................ .... NUMBER EXAMINED: NOT REMARKABLE: 4 1 7 2 --DILATATION, VENTRICULAR --COMPRESSION, VENTRAL --I-CARCINOMA 11 34 01 LIVER (LI) ........................................ .... NUMBER EXAMINED: 10 17 NOT REMARKABLE: 0 0 --INFILTRATE, LYMPHOHISTIOCYTIC --NECROSIS, INDIVIDUAL HEPATOCYTE --VACUOLATION, HEPATOCELLULAR, PERIPORTAL --FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL --FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC --DEGENERATION, CYSTIC --NECROSIS, COAGULATIVE --HEMATOPOIESIS, EXTRAMEDULLARY --HYPERPLASIA, BILE DUCT --FIBROSIS, PERIBILIARY --HYPERTROPHY, HEPATOCELLULAR, PERIPORTAL --INFILTRATE, MACROPHAGE, PIGMENTED 9 16 12 26 58 57 31 11 01 89 33 24 01 497 TABLE 41 Incidence of Microscopic Observations Recovery Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 2 TABLE INCLUDES: SEX=ALL;GROUP=6 ;WEEKS=ALL DEATH=U;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---SEX: MALE FEMALE GROUP: -6- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 10 17 ** FROM PREVIOUS PAGE ** LIVER (LI) ................................. ........... NUMBER EXAMINED: 10 NOT REMARKABLE: 0 17 0 --VACUOLATION, HEPATOCELLULAR --CYST, BILIARY --ANGIECTASIS --B-ADENOMA, HEPATOCELLULAR 21 02 02 01 LIVER-OIL RED O (LI0) ...................... .......... NUMBER EXAMINED: NOT REMARKABLE: 0 0 0 0 KIDNEY (KD) ....................................... .... NUMBER EXAMINED: NOT REMARKABLE: --NEPHROPATHY, CHRONIC PROGRESSIVE --DILATATION, PELVIC --MINERALIZATION, PELVIC --MINERALIZATION, TUBULAR --HYPERPLASIA, TRANSITIONAL CELL --INFLAMMATION, SUPPURATIVE, PELVIC --CYST SPLEEN (SP) ....................................... ... NUMBER EXAMINED: NOT REMARKABLE: --HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED --FIBROSIS, CAPSULAR 10 0 9 1 1 0 0 0 1 1 0 1 1 17 1 8 1 13 1 3 1 0 0 0 0 0 498 TABLE 41 Incidence of Microscopic Observations Recovery Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 3 TABLE INCLUDES: SEX=ALL;GROUP=6 ;WEEKS=ALL DEATH=U;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---SEX: MALE FEMALE GROUP: -6- -6NUMBER: 10 17 PANCREAS (PA) ................................... ..... NUMBER EXAMINED: NOT REMARKABLE: --ZYMOGEN GRANULES, DECREASED --INFILTRATE, MACROPHAGE, PIGMENTED --HYPERPLASIA, ISLET CELLS --ATROPHY, ACINAR --HYPERTROPHY / BASOPHILIA, ACINAR CELL --M-CARCINOMA, ISLET CELL --B-ADENOMA, ISLET CELL --INFILTRATION, FAT, INTERSTITIAL --INFLAMMATION, VASCULAR ADRENAL, CORTEX (AC) ............................ ..... NUMBER EXAMINED: NOT REMARKABLE: --HYPERTROPHY, CORTICAL CELL --DEGENERATION, CYSTIC / HEMORRHAGIC --PIGMENT --DEGENERATION / NECROSIS, UNILATERAL --M-CARCINOMA ADRENAL, MEDULLA (MA) .......................... ..... NUMBER EXAMINED: NOT REMARKABLE: 10 1 1 0 1 1 2 1 1 3 1 0 0 0 0 0 0 0 0 0 17 11 2 1 0 0 0 0 0 4 0 3 0 1 2 3 1 1 1 1 499 TABLE 41 Incidence of Microscopic Observations Recovery Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS TABLE INCLUDES: SEX=ALL;GROUP=6 ;WEEKS=ALL DEATH=U;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R SEX: MALE FEMALE GROUP: -6- -6NUMBER: 10 17 O F ANIMALS LN, MESENTERIC (MS) .................. --INFILTRATE, MACROPHAGE, PIGMENTED OVARY (OV) ............................ --CYST --HYPERPLASIA, GRANULOSA CELL TESTIS (TE) ...................................... --HYPERPLASIA, INTERSTITIAL CELL (LEYDIG CELL) BONE, FEMUR (FE) ................................. NUMBER EXAMINED: NOT REMARKABLE: NUMBER EXAMINED: NOT REMARKABLE: NUMBER EXAMINED: NOT REMARKABLE: NUMBER EXAMINED: NOT REMARKABLE: 0 0 0 0 0 0 0 9 8 1 0 0 1 0 1 8 2 4 2 0 0 0 0 0 MARROW, FEMUR (FM) ............................... NUMBER EXAMINED: NOT REMARKABLE: 0 0 0 0 MARROW, STERNUM (SE) ............................. NUMBER EXAMINED: NOT REMARKABLE: 0 0 0 0 Covance 6329-183 3M T-6295 PAGE: 4 A F F E C T E D -- 500 TABLE 41 Incidence of Microscopic Observations Recovery Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 5 TABLE INCLUDES: SEX=ALL;GROUP=6 ;WEEKS=ALL DEATH=U;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---SEX: MALE FEMALE GROUP: -6- -6NUMBER: 10 17 BONE, STERNUM (SB) ....................... ............ NUMBER EXAMINED: NOT REMARKABLE: 0 0 0 0 SPINAL CORD (SC) .................. ................... NUMBER EXAMINED: NOT REMARKABLE: 0 0 0 0 THYMUS (TH) ................................... ....... NUMBER EXAMINED: NOT REMARKABLE: --CYST --HYPERPLASIA, LYMPHORETICULAR --HYPERPLASIA, EPITHELIAL LUNG (LU) ..................................... ....... NUMBER EXAMINED: NOT REMARKABLE: --MINERALIZATION, VASCULAR --N-CARCINOMA --INFILTRATE, MACROPHAGE, ALVEOLAR --INFILTRATE, LYMPHOHISTIOCYTIC --MINERALIZATION, ALVEOLAR LUMINA --INFILTRATE, MACROPHAGE, PIGMENTED HEART (HT) .................................... ....... NUMBER EXAMINED: NOT REMARKABLE: 0 0 0 0 0 10 3 3 0 5 1 0 0 0 0 2 0 1 1 1 17 9 2 1 3 1 1 1 0 0 501 TABLE 41 Incidence of Microscopic Observations Recovery Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 6 TABLE INCLUDES: SEX=ALL;GROUP=6 ;WEEKS=ALL DEATH=U;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---SEX: MALE FEMALE GROUP: -6- -6NUMBER: 10 17 MUSCLE, SKELETAL (SM) .................... ............ NUMBER EXAMINED: NOT REMARKABLE: 0 0 0 0 NERVE, SCIATIC (SN) ................. ................. NUMBER EXAMINED: NOT REMARKABLE: 0 0 0 0 TRACHEA (TR) .................... ..................... NUMBER EXAMINED: NOT REMARKABLE: 0 0 0 0 ESOPHAGUS (ES) ................... .................... NUMBER EXAMINED: NOT REMARKABLE: 0 0 0 0 THYROID (TY) .... --HYPERPLASIA, C-CELL --M-CARCINOMA, FOLLICULAR CELL --DILATATION / CYST, FOLLICULAR --B-ADENOMA, C -CELL --B-ADENOMA, FOLLICULAR CELL --M-CARCINOMA, C-CELL ......... NUMBER EXAMINED: NOT REMARKABLE: 10 2 2 1 1 2 5 0 17 7 5 0 0 4 1 1 502 TABLE 41 Incidence of Microscopic Observations Recovery Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 7 TABLE INCLUDES: SEX=ALL;GROUP=6 ;WEEKS=ALL DEATH=U;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---SEX: MALE FEMALE GROUP: -6- -6NUMBER: 10 17 PARATHYROID (PT) ............................... ...... NUMBER EXAMINED: NOT REMARKABLE: --HYPERPLASIA PITUITARY (PI) ................................. ...... NUMBER EXAMINED: NOT REMARKABLE: 10 8 2 8 0 --B-ADENOMA --HYPERPLASIA --CYST --M-CARCINOMA HARDERIAN GLAND (HG) .......................... ...... NUMBER EXAMINED: NOT REMARKABLE: 7 1 0 0 0 0 16 16 0 11 0 9 3 1 1 0 0 SALIV GL, MANDIB (SG) ......................... ...... NUMBER EXAMINED: NOT REMARKABLE: 0 0 0 0 STOMACH, GL (ST) ............................... ...... NUMBER EXAMINED: NOT REMARKABLE: --EROSION --INFLAMMATION, CHRONIC-ACTIVE --ULCERATION --HYPERPLASIA, EPITHELIAL, GLANDULAR 4 1 0 1 1 3 4 2 1 1 0 0 503 TABLE 41 Incidence of Microscopic Observations Recovery Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 8 TABLE INCLUDES: SEX=ALL;GROUP=6 ;WEEKS=ALL DEATH=U;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---SEX: MALE FEMALE GROUP: -6- -6NUMBER: 10 17 STOMACH, NONGL (SU) ........ NUMBER EXAMINED: NOT REMARKABLE: 0 0 0 0 DUODENUM (DU) ............... NUMBER EXAMINED: NOT REMARKABLE: 0 0 0 0 JEJUNUM (JE) ................ --M-CARCINOMA ILEUM (IL) .................. NUMBER EXAMINED: 0 1 NOT REMARKABLE: 0 0 01 NUMBER EXAMINED: NOT REMARKABLE: 0 0 0 0 CECUM (CE) .................. NUMBER EXAMINED: NOT REMARKABLE: 1 1 0 0 COLON (CO) .................. NUMBER EXAMINED: NOT REMARKABLE: 0 0 0 0 RECTUM (RE) ................. NUMBER EXAMINED: NOT REMARKABLE: 0 0 0 0 504 TABLE 41 Incidence of Microscopic Observations Recovery Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 9 TABLE INCLUDES: SEX=ALL;GROUP=6 ;WEEKS=ALL DEATH=U;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---SEX: MALE FEMALE GROUP: -6- -6NUMBER: 10 17 SKIN (SK) .................................... ........ NUMBER EXAMINED: NOT REMARKABLE: --INFLAMMATION, CHRONIC-ACTIVE --B-KERATOACANTHOMA --B-PAPILLOMA, SQUAMOUS CELL MAMMARY, FEMALE (MF) ........................ ........ NUMBER EXAMINED: NOT REMARKABLE: --M-CARCINOMA --CYST / GALACTOCELE --INFLAMMATION, CHRONIC-ACTIVE --B-FIBROADENOMA --HYPERPLASIA, GLANDULAR --B-ADENOMA URINARY BLADDER (UB) ........................ ........ NUMBER EXAMINED: NOT REMARKABLE: --HEMORRHAGE --INFILTRATE, LYMPHOHISTIOCYTIC --HYPERPLASIA, TRANSITIONAL CELL UTERUS (UT) .................................. ........ NUMBER EXAMINED: NOT REMARKABLE: --DILATATION 3 0 1 2 1 0 0 0 0 0 0 0 0 10 9 0 1 0 0 0 0 2 0 1 1 1 17 0 1 7 2 7 11 2 17 15 1 2 1 6 1 2 505 TABLE 41 Incidence of Microscopic Observations Recovery Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 10 TABLE INCLUDES: SEX=ALL;GROUP=6 ;WEEKS=ALL DEATH=U;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C SEX: MALE FEMALE GROUP: -6- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 10 17 ** FROM PREVIOUS PAGE ** UTERUS (UT) ................................... ....... NUMBER EXAMINED: 0 6 NOT REMARKABLE: 0 1 --B-POLYP, ENDOMETRIAL, STROMAL --HYPERPLASIA, CYSTIC, ENDOMETRIAL --M-LEIOMYOSARCOMA 01 03 01 CERVIX (CV) ................................... ....... NUMBER EXAMINED: 0 1 NOT REMARKABLE: 0 0 --B-POLYP, STROMAL 01 VAGINA (VA) ................................... ....... NUMBER EXAMINED: NOT REMARKABLE: 0 0 0 0 T E D -- PROSTATE (PR) ................................. ....... NUMBER EXAMINED: NOT REMARKABLE: --INFLAMMATION, CHRONIC-ACTIVE SEMINAL VESICLES (SV) ........................ ....... NUMBER EXAMINED: NOT REMARKABLE: --SECRETION, DECREASED 1 0 1 1 0 1 0 0 0 0 0 0 506 TABLE 41 Incidence of Microscopic Observations Recovery Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 11 TABLE INCLUDES: SEX=ALL;GROUP=6;WEEKS=ALL DEATH=U;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R - O F - A N I M A L S - A F F E C SEX: MALE FEMALE GROUP: -6- -6- NUMBER: 10 17 T E D -- 0 0EPIDIDYMIDES (EP) ............................ ........ NUMBER EXAMINED: 0 0NOT REMARKABLE: 0 0HEMATO NEOPLASIA (HN) ....................... ........ NUMBER EXAMINED: 0 0NOT REMARKABLE: 10CAUSE OF DEATH (CD) ......................... ........ NUMBER EXAMINED: 0 0NOT REMARKABLE: 17 --SCHEDULED SACRIFICE 10 17 2 1LN, MEDIASTINAL (ML) ........................ ........ NUMBER EXAMINED: NOT REMARKABLE: 0 0 --HYPERPLASIA, LYMPHOPLASMACYTIC --LYMPHANGIECTASIS 11 10 1LN, AXILLARY (AX) ............................ ........ NUMBER EXAMINED: 4 NOT REMARKABLE: 1 2 --HYPERPLASIA, LYMPHOPLASMACYTIC 02 0 0LN, INGUINAL (IN) ............................ ........ NUMBER EXAMINED: 0 0NOT REMARKABLE: 507 TABLE 41 Incidence of Microscopic Observations Recovery Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 12 TABLE INCLUDES: SEX=ALL;GROUP=6 ;WEEKS=ALL DEATH=U;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R - O F - A N I M A L S - A F F E C SEX: MALE FEMALE GROUP: -6- -6NUMBER: 10 17 T E D -- LN, OTHER (LN) ................................ ....... NUMBER EXAMINED: NOT REMARKABLE: 0 0 0 0 LN, RENAL (RL) ................................ ....... NUMBER EXAMINED: NOT REMARKABLE: --INFILTRATE, MACROPHAGE, PIGMENTED --LYMPHANGIECTASIS LN, MANDIBULAR (MN) .......................... ....... NUMBER EXAMINED: NOT REMARKABLE: --HYPERPLASIA, LYMPHOPLASMACYTIC --HEMORRHAGE --LYMPHANGIECTASIS VERTEBRA/MARROW (VT) ......................... ....... NUMBER EXAMINED: NOT REMARKABLE: 1 0 1 1 1 0 1 1 1 0 0 0 0 0 0 5 0 3 4 0 0 0 MAMMARY, MALE (MM) ............................ ....... NUMBER EXAMINED: NOT REMARKABLE: 0 0 0 0 COAGULATING GL (CG) .......................... ....... NUMBER EXAMINED: NOT REMARKABLE: 0 0 0 0 508 TABLE 41 Incidence of Microscopic Observations Recovery Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 13 TABLE INCLUDES: SEX=ALL;GROUP=6 ;WEEKS=ALL DEATH=U;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R - O F - A N I M A L S - A F F E C SEX: MALE FEMALE GROUP: -6- -6NUMBER: 10 17 T E D -- TAIL (TI) .................................... ........ NUMBER EXAMINED: NOT REMARKABLE: --INFLAMMATION, CHRONIC-ACTIVE --ACANTHOSIS / HYPERKERATOSIS --SUPERFICIAL CRUSTING LN, LUMBAR/ILIAC (LM) ....................... ........ NUMBER EXAMINED: NOT REMARKABLE: --LYMPHANGIECTASIS --HYPERPLASIA, LYMPHOPLASMACYTIC --HEMORRHAGE CAVITY, ABDOM (PC) .......................... ........ NUMBER EXAMINED: NOT REMARKABLE: --INFLAMMATION, VASCULAR AUDITORY SEB GL (AS) ........................ ........ NUMBER EXAMINED: NOT REMARKABLE: 2 0 2 2 1 2 0 2 0 0 1 0 1 0 0 0 0 0 0 0 4 1 1 2 1 0 0 0 0 0 PREPUTIAL GLAND (PG) ........................ ........ NUMBER EXAMINED: NOT REMARKABLE: --INFLAMMATION, CHRONIC-ACTIVE --ECTASIA, DUCTAL 2 0 2 1 0 0 0 0 509 TABLE 41 Incidence of Microscopic Observations Recovery Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS TABLE INCLUDES: SEX=ALL;GROUP=6 ;WEEKS=ALL DEATH=U;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R SEX: MALE FEMALE GROUP: -6- -6NUMBER: 10 17 O F ANIMALS FOOT/FOOTPAD (FP) --INFLAMMATION, CHRONIC-ACTIVE --ULCERATION --ACANTHOSIS / HYPERKERATOSIS --FIBROSIS --HYPERPLASIA, BONE AORTA (AO) ...................... NUMBER EXAMINED: NOT REMARKABLE: NUMBER EXAMINED: NOT REMARKABLE: 1 0 1 0 0 1 1 0 0 1 0 1 1 1 1 0 0 0 CALVARIUM/SKULL (CA) NUMBER EXAMINED: NOT REMARKABLE: 0 0 0 0 LN, ANT MES/PANC (AP) NUMBER EXAMINED: NOT REMARKABLE: 1 1 0 0 HEAD, CORONAL (HC) NUMBER EXAMINED: NOT REMARKABLE: 0 0 0 0 Covance 6329-183 3M T-6295 PAGE: 14 A F F E C T E D -- 510 TABLE 41 Incidence of Microscopic Observations Recovery Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 15 TABLE INCLUDES: SEX=ALL;GROUP=6 ;WEEKS=ALL DEATH=U;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R - O F - A N I M A L S - A F F E C SEX: MALE FEMALE GROUP: -6- -6NUMBER: 10 17 T E D -- JOINT, OTHER (JT) --INFLAMMATION, CHRONIC-ACTIVE --FIBROSIS --HYPERPLASIA, BONE CLITORAL GLAND (CL) ............. NUMBER EXAMINED: NOT REMARKABLE: NUMBER EXAMINED: NOT REMARKABLE: 3 0 3 1 1 0 0 0 0 0 0 0 0 0 URETER (UE) NUMBER EXAMINED: NOT REMARKABLE: 0 0 0 0 ADIPOSE TISSUE (AT) NUMBER EXAMINED: NOT REMARKABLE: 0 0 0 0 DIAPHRAGM (DP) NUMBER EXAMINED: NOT REMARKABLE: 0 0 0 0 CAVITY, THORACIC (TA) NUMBER EXAMINED: NOT REMARKABLE: 0 0 0 0 511 TABLE 41 Incidence of Microscopic Observations Recovery Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS TABLE INCLUDES: SEX=ALL;GROUP=6 ;WEEKS=ALL DEATH=U;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B SEX: MALE FEMALE GROUP: -6- -6NUMBER: 10 17 O F ANIMALS LN, TRACHEOBRON (TB) ......... NUMBER EXAMINED: NOT REMARKABLE: 0 0 0 0 LN, POPLITEAL (PP) ............ --HYPERPLASIA, PLASMACYTIC MUSCLE, OTHER (MO) ............ NUMBER EXAMINED: NOT REMARKABLE: NUMBER EXAMINED: NOT REMARKABLE: 1 0 1 0 0 0 0 0 0 0 PERICARDIAL SAC (PD) ......... NUMBER EXAMINED: NOT REMARKABLE: 0 0 0 0 PINNA (PN) .................... --B-PAPILLOMA, SQUAMOUS CELL ARTERY, OTHER (AU) ............ NUMBER EXAMINED: 0 1 NOT REMARKABLE: 0 0 01 NUMBER EXAMINED: NOT REMARKABLE: 0 0 0 0 Covance 6329-183 3M T-6295 PAGE: 16 A F F E C T E D -- 512 TABLE 41 Incidence of Microscopic Observations Recovery Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 17 TABLE INCLUDES: SEX=ALL;GROUP=6 ;WEEKS=ALL DEATH=U;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R - O F - A N I M A L S - A F F E C SEX: MALE FEMALE GROUP: -6- -6NUMBER: 10 17 T E D -- AORTIC ARCH (AA) .... LN, ILEOCECAL (IC) .. NUMBER EXAMINED: NOT REMARKABLE: NUMBER EXAMINED: NOT REMARKABLE: 00 00 00 00 VAS DEFERENS (VS) ... NUMBER EXAMINED: NOT REMARKABLE: 00 00 BILE DUCT (BD) ..... NUMBER EXAMINED: NOT REMARKABLE: 00 00 TRIGEMINAL NERVE (TG) ** END OF LIST ** NUMBER EXAMINED: NOT REMARKABLE: 00 00 513 APPENDIX 1 Protocol Deviations Covance 6329-183 3M T-6295 514 Protocol Deviations Covance 6329-183 3M T-6295 Protocol. Husbandry. Diet. "Certified Rodent Diet #5002, meal (PMI Nutrition International) ad libitum." Actual Procedure. During the course of the study, for a total of 29 incidences, 24 animals were found without access to food. Food was provided when the limited access was noted. Comments regarding limited access to food are recorded in the data. During single housing on April 13, 1998 (acclimation), temporary Animal No. 593 was not fed in. Protocol. Husbandry. Water. "Ad libitum." Actual Procedure. On April 29, 1998, during the a.m. room check it was noticed that Animal Nos. C92671 through C92690 (Group 3 males), and C92691 through C92725 (Group 4 males) did not have access to water due to a kinked water hose. Protocol. Husbandry. Housing. "Individual (may be group-housed during acclimation)." Actual Procedure. On May 10, 1998, Animal No. C92989 (Group 2 female) was found out of its cage during the a.m. check. Protocol. Termination. Unscheduled Sacrifices and Deaths. "Animals to be sacrificed will be anesthetized with carbon dioxide, weighed, and exsanguinated." Actual Procedure. At necropsy, Animal Nos. C92918 (Group 1 female), C92939 (Group 2 female), and C93038 (Group 3 female) were anesthetized with sodium pentobarbital instead of carbon dioxide. At necropsy, Animal No. C92621 received approximately 1.0mL of sodium pentobarbital prior to administration of carbon dioxide anesthesia. A body weight was not taken for Animal Nos. C92566 (Group 1 male), C92687 (Group 3 male) and C92966 (Group 2 female) prior to necropsy. 515 Protocol Deviations (Continued) Covance 6329-183 3M T-6295 Protocol. Postmortem Procedures. Tissue Preservation. "At the terminal sacrifice, sections of the heart and liver will be collected from 10 animals/sex/group in Groups 1, 4, 5, and 6 and preserved in 2.0% paraformaldehyde/2.5% glutaraldehyde in 0.1 M phosphate buffer. These tissues will be processed and embedded in epoxy blocks for possible future examination by electron microscopy. Actual Procedure. At the terminal sacrifice, tissues were collected from 11 animals in Group 4 (females) for possible electron microscopy instead of 10 animals. Protocol. Postmortem Procedures. Histopathology. "Lesions, liver, lungs, kidneys, pancreas, thyroid, testes, and mammary glands (females) from each animal in Groups 2, 3, 4, and 6 sacrificed at the terminal and recovery sacrifices will be embedded in paraffin, sectioned, stained with hematoxylin and eosin, and examined microscopically." Actual Procedure. At the terminal sacrifice, one mammary gland mass was not collected for Animal Nos. C92927 (Group 1 female) and C93078 (Group 4 female). At necropsy, the mass located in the inguinal region for Animal No. C92729 was not commented on or collected. On July 13, 1999, a macroscopic lesion for Animal No. C92838 was not collected at the time of necropsy. At the terminal and recovery sacrifices, parathyroid glands were examined microscopically from animals in Groups 2, 3, 4. Protocol. Postmortem Procedures. Histopathology. "Slides of the pancreas from males found dead, sacrificed at an unscheduled interval, or sacrificed at the Week 14 and 53 interim sacrifices, terminal, and recovery sacrifices will be sent to Tox Path Inc. for peer-review and results will be provided to Covance for inclusion in the final report." Actual Procedure. Results of the peer-review are not included in the final report. The results are on file with Covance. 516 Protocol Deviations (Continued) Covance 6329-183 3M T-6295 Protocol. Experimental Design. Postmortem Procedures. "The following tissues (when present) or representative samples will be collected from each animal and preserved in 10% neutral-buffered formalin, unless otherwise specified." Actual Procedure. Some tissues, required by the protocol, were not available for histopathologic examination. Missing tissues are listed with appropriate comments in the pathology data sheets for individual animals. Summary tables do not include them as having been examined. These deviations are not expected to have affected the results of the study. 517 COVAH^ THE DEVELOPMENT SERVICES COMPANY Sponsor: 3M St. Paul, Minnesota FINAL REPORT Study Title: 104-Week Dietary Chronic Toxicity and Carcinogenicity Study with Perfluorooctane Sulfonic Acid Potassium Salt (PFOS; T-6295) in Rats Author: Peter J. Thomford, PhD Study Completion Date: January 2, 2002 Testing Facility: Covance Laboratories Inc. 3301 Kinsman Boulevard Madison, Wisconsin 53704-2595 Laboratory Study Identification: Covance 6329-183 Sponsor Study Identification: 3M T-6295 Volume II of IX Page 518 of 4068 Covance 6329-183 3M T-6295 VOLUME II OF IX CONTENTS Page APPENDIX 2 .................................................................................................................. 520 Individual Animal Fate Data....................................................................................... 521 Individual Clinical Observations................................................................................. 543 519 APPENDIX 2 Individual Animal Fate Data Individual Clinical Observations Covance 6329-183 3M T-6295 520 APPENDIX 2 Individual Animal Fate Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL DOSE NUMBER GROUP C92501 C92502 C92503 C92504 C92505 C92506 C92507 C92508 C92509 C92510 C92511 C92512 C92513 C92514 C92515 C92516 C92517 C92518 C92519 C92520 C92521 C92522 C92523 C92524 C92525 C92526 C92527 C92528 C92529 C92530 C92531 C92532 C92533 C92534 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 SEX MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE DEATH CODE TYPE OF DEATH DESCRIPTION OF DEATH 3 SCHEDULED THIRD INTERIM SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE 3 SCHEDULED THIRD INTERIM SACRIFICE T SCHEDULED TERMINAL SACRIFICE 1 SCHEDULED FIRST INTERIM SACRIFICE 3 SCHEDULED THIRD INTERIM SACRIFICE D UNSCHEDULED UNSCHEDULED DEATH 2 SCHEDULED SECOND INTERIM SACRIFICE T SCHEDULED TERMINAL SACRIFICE 2 SCHEDULED SECOND INTERIM SACRIFICE 3 SCHEDULED THIRD INTERIM SACRIFICE T SCHEDULED TERMINAL SACRIFICE T SCHEDULED TERMINAL SACRIFICE D UNSCHEDULED UNSCHEDULED DEATH D UNSCHEDULED UNSCHEDULED DEATH 3 SCHEDULED THIRD INTERIM SACRIFICE T SCHEDULED TERMINAL SACRIFICE D UNSCHEDULED UNSCHEDULED DEATH D UNSCHEDULED UNSCHEDULED DEATH 2 SCHEDULED SECOND INTERIM SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH 3 SCHEDULED THIRD INTERIM SACRIFICE 1 SCHEDULED FIRST INTERIM SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH 1 SCHEDULED FIRST INTERIM SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH 2 SCHEDULED SECOND INTERIM SACRIFICE 1 SCHEDULED FIRST INTERIM SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH 2 SCHEDULED SECOND INTERIM SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH 3 SCHEDULED THIRD INTERIM SACRIFICE DATE OF DAY OF DEATH STUDY 04/20/99 10/04/99 04/21/00 04/20/99 04/19/00 05/15/98 04/20/99 10/08/99 07/21/98 04/20/00 07/21/98 04/20/99 04/20/00 04/21/00 03/12/00 03/11/99 04/20/99 04/19/00 10/28/99 11/18/99 07/21/98 02/29/00 04/20/99 05/15/98 08/30/99 05/15/98 11/24/99 07/21/98 05/15/98 10/18/99 08/03/99 07/21/98 11/29/99 04/20/99 366 533 733 366 731 26 366 537 93 732 93 366 732 733 693 326 366 731 557 578 93 681 366 26 498 26 584 93 26 547 471 93 589 366 WEEK OF STUDY 53 77 105 53 105 4 53 77 14 105 14 53 105 105 99 47 53 105 80 83 14 98 53 4 72 4 84 14 4 79 68 14 85 53 PAGE: 1 521 APPENDIX 2 Individual Animal Fate Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL DOSE NUMBER GROUP C92535 C92536 C92537 C92538 C92539 C92540 C92541 C92542 C92543 C92544 C92545 C92546 C92547 C92548 C92549 C92550 C92551 C92552 C92553 C92554 C92555 C92556 C92557 C92558 C92559 C92560 C92561 C92562 C92563 C92564 C92565 C92566 C92567 C92568 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 SEX MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE DEATH CODE TYPE OF DEATH DESCRIPTION OF DEATH M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH D UNSCHEDULED UNSCHEDULED DEATH D UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH 3 SCHEDULED THIRD INTERIM SACRIFICE T SCHEDULED TERMINAL SACRIFICE T SCHEDULED TERMINAL SACRIFICE D UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE D UNSCHEDULED UNSCHEDULED DEATH 1 SCHEDULED FIRST INTERIM SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH D UNSCHEDULED UNSCHEDULED DEATH D UNSCHEDULED UNSCHEDULED DEATH D UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH D UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH 3 SCHEDULED THIRD INTERIM SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH D UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH D UNSCHEDULED UNSCHEDULED DEATH D UNSCHEDULED UNSCHEDULED DEATH DATE OF DEATH 02/14/00 10/17/99 02/14/00 09/14/99 02/28/00 04/20/99 04/20/00 04/21/00 03/14/00 04/20/00 01/31/00 05/15/98 12/21/99 11/22/99 10/31/99 04/15/00 04/19/00 04/14/00 06/28/99 04/07/00 06/08/99 06/28/99 12/27/99 10/28/99 10/22/99 10/31/99 06/29/99 04/20/99 11/24/99 08/19/99 01/16/00 09/21/98 01/12/00 03/20/00 DAY OF STUDY 666 546 666 513 680 366 732 733 695 732 652 26 611 582 560 727 731 726 435 719 415 435 617 557 551 560 436 366 584 487 637 155 633 701 WEEK OF STUDY 96 78 96 74 98 53 105 105 100 105 94 4 88 84 80 104 105 104 63 103 60 63 89 80 79 80 63 53 84 70 91 23 91 101 PAGE: 2 522 APPENDIX 2 Individual Animal Fate Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL DOSE NUMBER GROUP C92569 C92570 C92571 C92572 C92573 C92574 C92575 C92576 C92577 C92578 C92579 C92580 C92581 C92582 C92583 C92584 C92585 C92586 C92587 C92588 C92589 C92590 C92591 C92592 C92593 C92594 C92595 C92596 C92597 C92598 C92599 C92600 C92601 C92602 1 1 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 SEX MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE DEATH CODE TYPE OF DEATH DESCRIPTION OF DEATH T SCHEDULED TERMINAL SACRIFICE 3 SCHEDULED THIRD INTERIM SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH D UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH 1 SCHEDULED FIRST INTERIM SACRIFICE 1 SCHEDULED FIRST INTERIM SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH D UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE D UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH 2 SCHEDULED SECOND INTERIM SACRIFICE D UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE T SCHEDULED TERMINAL SACRIFICE D UNSCHEDULED UNSCHEDULED DEATH 2 SCHEDULED SECOND INTERIM SACRIFICE T SCHEDULED TERMINAL SACRIFICE T SCHEDULED TERMINAL SACRIFICE DATE OF DAY OF DEATH STUDY 04/19/00 04/20/99 03/21/00 03/21/00 11/24/99 05/15/98 05/15/98 02/21/00 04/21/00 12/08/99 12/12/99 10/20/99 04/17/00 03/21/00 04/19/00 06/11/99 01/12/99 09/07/99 12/20/99 04/03/00 03/13/00 04/20/00 02/18/00 12/20/99 07/21/98 02/15/00 11/23/99 08/23/99 04/19/00 04/19/00 05/09/99 07/21/98 04/21/00 04/21/00 731 366 702 702 584 26 26 673 733 598 602 549 729 702 731 418 268 506 610 715 694 732 670 610 93 667 583 491 731 731 385 93 733 733 WEEK OF STUDY 105 53 101 101 84 4 4 97 105 86 86 79 105 101 105 60 39 73 88 103 100 105 96 88 14 96 84 71 105 105 55 14 105 105 PAGE: 3 523 APPENDIX 2 Individual Animal Fate Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL DOSE NUMBER GROUP C92603 C92604 C92605 C92606 C92607 C92608 C92609 C92610 C92611 C92612 C92613 C92614 C92615 C92616 C92617 C92618 C92619 C92620 C92621 C92622 C92623 C92624 C92625 C92626 C92627 C92628 C92629 C92630 C92631 C92632 C92633 C92634 C92635 C92636 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 3 3 3 3 3 3 SEX MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE DEATH CODE TYPE OF DEATH DESCRIPTION OF DEATH M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH D UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH 1 SCHEDULED FIRST INTERIM SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH 1 SCHEDULED FIRST INTERIM SACRIFICE D UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH 2 SCHEDULED SECOND INTERIM SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH 1 SCHEDULED FIRST INTERIM SACRIFICE T SCHEDULED TERMINAL SACRIFICE D UNSCHEDULED UNSCHEDULED DEATH 2 SCHEDULED SECOND INTERIM SACRIFICE D UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE D UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE 2 SCHEDULED SECOND INTERIM SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH D UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE D UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH D UNSCHEDULED UNSCHEDULED DEATH DATE OF DAY OF DEATH STUDY 08/24/98 0 1 / 10/00 04/11/00 10/13/99 0 1 / 10/00 10/19/99 04/12/00 05/15/98 12/20/99 10/18/99 05/15/98 01/19/00 01/03/00 07/21/98 11/05/99 05/15/98 04/20/00 02/15/00 07/21/98 11/18/99 04/21/00 06/18/99 11/28/99 04/20/00 07/21/98 09/07/99 10/17/99 12/26/99 04/19/00 04/03/00 04/20/00 12/13/99 10/20/99 03/01/00 127 631 723 542 631 548 724 26 610 547 26 640 624 93 565 26 732 667 93 578 733 425 588 732 93 506 546 616 731 715 732 603 549 682 WEEK OF STUDY 19 91 104 78 91 79 104 4 88 79 4 92 90 14 81 4 105 96 14 83 105 61 84 105 14 73 78 88 105 103 105 87 79 98 PAGE: 4 524 APPENDIX 2 Individual Animal Fate Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL DOSE NUMBER GROUP C92637 C92638 C92639 C92640 C92641 C92642 C92643 C92644 C92645 C92646 C92647 C92648 C92649 C92650 C92651 C92652 C92653 C92654 C92655 C92656 C92657 C92658 C92659 C92660 C92661 C92662 C92663 C92664 C92665 C92666 C92667 C92668 C92669 C92670 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 SEX MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE DEATH CODE TYPE OF DEATH DESCRIPTION OF DEATH T SCHEDULED TERMINAL SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH 2 SCHEDULED SECOND INTERIM SACRIFICE T SCHEDULED TERMINAL SACRIFICE T SCHEDULED TERMINAL SACRIFICE T SCHEDULED TERMINAL SACRIFICE T SCHEDULED TERMINAL SACRIFICE 2 SCHEDULED SECOND INTERIM SACRIFICE 1 SCHEDULED FIRST INTERIM SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE 1 SCHEDULED FIRST INTERIM SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH 1 SCHEDULED FIRST INTERIM SACRIFICE T SCHEDULED TERMINAL SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE D UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE T SCHEDULED TERMINAL SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH 2 SCHEDULED SECOND INTERIM SACRIFICE D UNSCHEDULED UNSCHEDULED DEATH D UNSCHEDULED UNSCHEDULED DEATH D UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH 1 SCHEDULED FIRST INTERIM SACRIFICE T SCHEDULED TERMINAL SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH DATE OF DAY OF DEATH STUDY 04/19/00 0 1 / 10/00 11/29/99 07/21/98 04/20/00 04/19/00 04/21/00 04/19/00 07/21/98 05/15/98 10/28/99 12/15/99 04/20/00 05/15/98 11/01/99 05/15/98 04/21/00 10/20/99 02/01/00 02/21/00 04/21/00 02/01/00 04/20/00 04/20/00 01/03/00 07/21/98 02/08/00 10/11/99 12/24/99 10/01/99 10/20/99 05/15/98 04/20/00 09/13/99 731 631 589 93 732 731 733 731 93 26 557 605 732 26 561 26 733 549 653 673 733 653 732 732 624 93 660 540 614 530 549 26 732 512 WEEK OF STUDY 105 91 85 14 105 105 105 105 14 4 80 87 105 4 81 4 105 79 94 97 105 94 105 105 90 14 95 78 88 76 79 4 105 74 PAGE: 5 525 APPENDIX 2 Individual Animal Fate Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL DOSE NUMBER GROUP C92671 C92672 C92673 C92674 C92675 C92676 C92677 C92678 C92679 C92680 C92681 C92682 C92683 C92684 C92685 C92686 C92687 C92688 C92689 C92690 C92691 C92692 C92693 C92694 C92695 C92696 C92697 C92698 C92699 C92700 C92701 C92702 C92703 C92704 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 4 4 4 4 4 4 4 4 4 4 4 4 4 4 SEX MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE DEATH CODE TYPE OF DEATH DESCRIPTION OF DEATH D UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH D UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH 2 SCHEDULED SECOND INTERIM SACRIFICE D UNSCHEDULED UNSCHEDULED DEATH 1 SCHEDULED FIRST INTERIM SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE T SCHEDULED TERMINAL SACRIFICE 2 SCHEDULED SECOND INTERIM SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH D UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE T SCHEDULED TERMINAL SACRIFICE D UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE T SCHEDULED TERMINAL SACRIFICE T SCHEDULED TERMINAL SACRIFICE T SCHEDULED TERMINAL SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE T SCHEDULED TERMINAL SACRIFICE T SCHEDULED TERMINAL SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE D UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE DATE OF DAY OF DEATH STUDY 01/29/00 02/13/00 02/07/00 04/19/00 03/31/00 07/21/98 03/09/00 05/15/98 05/07/99 11/23/99 12/16/99 04/19/00 04/21/00 07/21/98 10/25/99 11/22/99 09/03/99 02/25/00 05/14/99 04/21/00 04/19/00 04/19/99 04/21/00 04/19/00 04/19/00 04/19/00 07/28/99 04/20/00 04/19/00 04/21/00 04/19/99 04/20/00 06/16/99 04/20/00 650 665 659 731 712 93 690 26 383 583 606 731 733 93 554 582 502 677 390 733 731 365 733 731 731 731 465 732 731 733 365 732 423 732 WEEK OF STUDY 93 95 95 105 102 14 99 4 55 84 87 105 105 14 80 84 72 97 56 105 105 53 105 105 105 105 67 105 105 105 53 105 61 105 PAGE: 6 526 APPENDIX 2 Individual Animal Fate Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL DOSE NUMBER GROUP C92705 C92706 C92707 C92708 C92709 C92710 C92711 C92712 C92713 C92714 C92715 C92716 C92717 C92718 C92719 C92720 C92721 C92722 C92723 C92724 C92725 C92726 C92727 C92728 C92729 C92730 C92731 C92732 C92733 C92734 C92735 C92736 C92737 C92738 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 SEX MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE DEATH CODE TYPE OF DEATH DESCRIPTION OF DEATH T SCHEDULED TERMINAL SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE D UNSCHEDULED UNSCHEDULED DEATH D UNSCHEDULED UNSCHEDULED DEATH D UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH 2 SCHEDULED SECOND INTERIM SACRIFICE 2 SCHEDULED SECOND INTERIM SACRIFICE 1 SCHEDULED FIRST INTERIM SACRIFICE T SCHEDULED TERMINAL SACRIFICE T SCHEDULED TERMINAL SACRIFICE 1 SCHEDULED FIRST INTERIM SACRIFICE 2 SCHEDULED SECOND INTERIM SACRIFICE D UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH 2 SCHEDULED SECOND INTERIM SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE D UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE D UNSCHEDULED UNSCHEDULED DEATH 2 SCHEDULED SECOND INTERIM SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE 1 SCHEDULED FIRST INTERIM SACRIFICE D UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH 1 SCHEDULED FIRST INTERIM SACRIFICE T SCHEDULED TERMINAL SACRIFICE T SCHEDULED TERMINAL SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH D UNSCHEDULED UNSCHEDULED DEATH DATE OF DAY OF DEATH STUDY 04/19/00 02/28/00 01/06/00 04/19/00 01/12/00 08/18/99 01/19/00 12/13/99 07/21/98 07/21/98 05/15/98 04/20/00 04/21/00 05/15/98 07/21/98 11/16/99 10/28/99 07/21/98 10/23/99 04/19/00 04/17/99 04/21/00 01/09/00 07/21/98 10/20/99 04/20/00 05/15/98 11/18/99 12/27/99 05/15/98 04/19/00 04/21/00 01/16/00 0 1 /20/00 731 680 627 731 633 486 640 603 93 93 26 732 733 26 93 576 557 93 552 731 363 733 630 93 549 732 26 578 617 26 731 733 637 641 WEEK OF STUDY 105 98 90 105 91 70 92 87 14 14 4 105 105 4 14 83 80 14 79 105 52 105 90 14 79 105 4 83 89 4 105 105 91 92 PAGE: 7 527 APPENDIX 2 Individual Animal Fate Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL DOSE NUMBER GROUP C92739 C92740 C92741 C92742 C92743 C92744 C92745 C92746 C92747 C92748 C92749 C92750 C92751 C92752 C92753 C92754 C92755 C92756 C92757 C92758 C92759 C92760 C92761 C92762 C92763 C92764 C92765 C92766 C92767 C92768 C92769 C92770 C92771 C92772 4 4 4 4 4 4 4 4 4 4 4 4 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 SEX MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE DEATH CODE TYPE OF DEATH DESCRIPTION OF DEATH T SCHEDULED TERMINAL SACRIFICE T SCHEDULED TERMINAL SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE D UNSCHEDULED UNSCHEDULED DEATH 1 SCHEDULED FIRST INTERIM SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE T SCHEDULED TERMINAL SACRIFICE D UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE 3 SCHEDULED THIRD INTERIM SACRIFICE 1 SCHEDULED FIRST INTERIM SACRIFICE T SCHEDULED TERMINAL SACRIFICE 3 SCHEDULED THIRD INTERIM SACRIFICE 3 SCHEDULED THIRD INTERIM SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH 1 SCHEDULED FIRST INTERIM SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE T SCHEDULED TERMINAL SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH 2 SCHEDULED SECOND INTERIM SACRIFICE D UNSCHEDULED UNSCHEDULED DEATH D UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH D UNSCHEDULED UNSCHEDULED DEATH D UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH DATE OF DAY OF DEATH STUDY 04/21/00 04/21/00 01/12/00 04/20/00 11/27/99 05/15/98 01/03/00 10/22/99 04/20/00 04/20/00 04/06/00 04/20/00 04/20/99 05/15/98 04/21/00 04/20/99 04/20/99 12/24/99 04/19/00 06/21/99 05/15/98 09/13/99 04/20/00 04/19/00 02/01/00 10/01/99 07/21/98 03/24/00 03/10/00 02/08/00 04/09/00 03/21/00 11/07/99 04/14/99 733 733 633 732 587 26 624 551 732 732 718 732 366 26 733 366 366 614 731 428 26 512 732 731 653 530 93 705 691 660 721 702 567 360 WEEK OF STUDY 105 105 91 105 84 4 90 79 105 105 103 105 53 4 105 53 53 88 105 62 4 74 105 105 94 76 14 101 99 95 103 101 81 52 PAGE: 8 528 APPENDIX 2 Individual Animal Fate Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL DOSE NUMBER GROUP C92773 C92774 C92775 C92776 C92777 C92778 C92779 C92780 C92781 C92782 C92783 C92784 C92785 C92786 C92787 C92788 C92789 C92790 C92791 C92792 C92793 C92794 C92795 C92796 C92797 C92798 C92799 C92800 C92801 C92802 C92803 C92804 C92805 C92806 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 SEX MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE DEATH CODE TYPE OF DEATH DESCRIPTION OF DEATH D UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE T SCHEDULED TERMINAL SACRIFICE T SCHEDULED TERMINAL SACRIFICE 2 SCHEDULED SECOND INTERIM SACRIFICE 3 SCHEDULED THIRD INTERIM SACRIFICE 1 SCHEDULED FIRST INTERIM SACRIFICE T SCHEDULED TERMINAL SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH D UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE D UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE 2 SCHEDULED SECOND INTERIM SACRIFICE 3 SCHEDULED THIRD INTERIM SACRIFICE T SCHEDULED TERMINAL SACRIFICE T SCHEDULED TERMINAL SACRIFICE 1 SCHEDULED FIRST INTERIM SACRIFICE D UNSCHEDULED UNSCHEDULED DEATH 3 SCHEDULED THIRD INTERIM SACRIFICE 3 SCHEDULED THIRD INTERIM SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH 1 SCHEDULED FIRST INTERIM SACRIFICE 2 SCHEDULED SECOND INTERIM SACRIFICE T SCHEDULED TERMINAL SACRIFICE D UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE T SCHEDULED TERMINAL SACRIFICE T SCHEDULED TERMINAL SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH DATE OF DAY OF DEATH STUDY 04/19/00 04/21/00 04/20/00 04/20/00 07/21/98 04/20/99 05/15/98 04/21/00 10/06/99 04/20/00 10/18/99 10/27/99 03/05/00 04/19/00 03/13/99 04/21/00 07/21/98 04/20/99 04/19/00 04/20/00 05/15/98 05/26/99 04/20/99 04/20/99 02/01/00 05/15/98 07/21/98 04/19/00 05/17/99 04/19/00 04/21/00 04/19/00 09/01/99 03/10/00 731 733 732 732 93 366 26 733 535 732 547 556 686 731 328 733 93 366 731 732 26 402 366 366 653 26 93 731 393 731 733 731 500 691 WEEK OF STUDY 105 105 105 105 14 53 4 105 77 105 79 80 98 105 47 105 14 53 105 105 4 58 53 53 94 4 14 105 57 105 105 105 72 99 PAGE: 9 529 APPENDIX 2 Individual Animal Fate Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL DOSE NUMBER GROUP C92807 C92808 C92809 C92810 C92811 C92812 C92813 C92814 C92815 C92816 C92817 C92818 C92819 C92820 C92821 C92822 C92823 C92824 C92825 C92826 C92827 C92828 C92829 C92830 C92831 C92832 C92833 C92834 C92835 C92836 C92837 C92838 C92839 C92840 5 5 5 5 5 5 5 5 5 5 5 5 5 5 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 SEX MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE DEATH CODE TYPE OF DEATH DESCRIPTION OF DEATH D UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE T SCHEDULED TERMINAL SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH 2 SCHEDULED SECOND INTERIM SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE T SCHEDULED TERMINAL SACRIFICE D UNSCHEDULED UNSCHEDULED DEATH 3 SCHEDULED THIRD INTERIM SACRIFICE T SCHEDULED TERMINAL SACRIFICE D UNSCHEDULED UNSCHEDULED DEATH 3 SCHEDULED THIRD INTERIM SACRIFICE U SCHEDULED FIRST POST-RECOV SAC M UNSCHEDULED UNSCHEDULED DEATH U SCHEDULED FIRST POST-RECOV SAC D UNSCHEDULED UNSCHEDULED DEATH D UNSCHEDULED UNSCHEDULED DEATH D UNSCHEDULED UNSCHEDULED DEATH U SCHEDULED FIRST POST-RECOV SAC D UNSCHEDULED UNSCHEDULED DEATH U SCHEDULED FIRST POST-RECOV SAC M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH D UNSCHEDULED UNSCHEDULED DEATH U SCHEDULED FIRST POST-RECOV SAC U SCHEDULED FIRST POST-RECOV SAC M UNSCHEDULED UNSCHEDULED DEATH D UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH D UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH DATE OF DAY OF DEATH STUDY 12/07/99 04/19/00 04/21/00 02/21/00 12/25/98 07/21/98 03/13/00 04/21/00 04/20/00 07/09/99 04/20/99 04/20/00 04/15/00 04/20/99 04/24/00 11/06/99 04/24/00 02/11/00 02/01/00 05/09/99 04/24/00 11/18/99 04/24/00 03/09/00 11/29/99 08/12/99 04/24/00 04/24/00 09/23/99 0 1 / 10/00 07/12/99 07/13/99 0 1 / 02/00 04/03/00 597 731 733 673 250 93 694 733 732 446 366 732 727 366 736 566 736 663 653 385 736 578 736 690 589 480 736 736 522 631 449 450 623 715 WEEK OF STUDY 86 105 105 97 36 14 100 105 105 64 53 105 104 53 106 81 106 95 94 55 106 83 106 99 85 69 106 106 75 91 65 65 89 103 PAGE: 10 530 APPENDIX 2 Individual Animal Fate Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER C92841 C92842 C92843 C92844 C92845 C92846 C92847 C92848 C92849 C92850 C92851 C92852 C92853 C92854 C92855 C92856 C92857 C92858 C92859 C92860 C92861 C92862 C92863 C92864 C92865 C92866 C92867 C92868 C92869 C92870 C92871 C92872 C92873 C92874 DOSE GROUP 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 1 1 1 1 1 1 1 1 1 1 1 1 1 1 SEX MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE DEATH CODE TYPE OF DEATH DESCRIPTION OF DEATH D UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH D UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH D UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH U SCHEDULED FIRST POST-RECOV SAC M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH U SCHEDULED FIRST POST-RECOV SAC M UNSCHEDULED UNSCHEDULED DEATH U SCHEDULED FIRST POST-RECOV SAC D UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH U SCHEDULED FIRST POST-RECOV SAC M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH 1 SCHEDULED FIRST INTERIM SACRI FICE 3 SCHEDULED THIRD INTERIM SACRI FICE M UNSCHEDULED UNSCHEDULED DEATH 1 SCHEDULED FIRST INTERIM SACRI FICE D UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE T SCHEDULED TERMINAL SACRIFICE T SCHEDULED TERMINAL SACRIFICE T SCHEDULED TERMINAL SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE T SCHEDULED TERMINAL SACRIFICE DATE OF DAY OF DEATH STUDY 08/08/99 01/12/00 10/08/99 11/19/99 02/12/00 04/09/00 02/28/00 04/24/00 08/16/99 04/18/00 04/24/00 08/07/99 04/24/00 03/24/00 08/16/99 04/24/00 11/16/99 02/21/00 09/01/99 02/26/00 05/15/98 04/20/99 11/15/99 05/15/98 07/12/99 04/21/00 04/20/00 04/19/00 04/20/00 04/17/00 04/19/00 12/28/99 04/21/00 04/19/00 476 633 537 579 664 721 680 736 484 730 736 475 736 705 484 736 576 673 500 678 26 366 575 26 449 733 732 731 732 729 731 618 733 731 WEEK OF STUDY 68 91 77 83 95 103 98 106 70 105 106 68 106 101 70 106 83 97 72 97 4 53 83 4 65 105 105 105 105 105 105 89 105 105 PAGE: 11 531 APPENDIX 2 Individual Animal Fate Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER C92875 C92876 C92877 C92878 C92879 C92880 C92881 C92882 C92883 C92884 C92885 C92886 C92887 C92888 C92889 C92890 C92891 C92892 C92893 C92894 C92895 C92896 C92897 C92898 C92899 C92900 C92901 C92902 C92903 C92904 C92905 C92906 C92907 C92908 DOSE GROUP 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 SEX FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE DEATH CODE TYPE OF DEATH DESCRIPTION OF DEATH M UNSCHEDULED UNSCHEDULED DEATH 3 SCHEDULED THIRD INTERIM SACRIFICE T SCHEDULED TERMINAL SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH 2 SCHEDULED SECOND INTERIM SACRIFICE T SCHEDULED TERMINAL SACRIFICE T SCHEDULED TERMINAL SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH 2 SCHEDULED SECOND INTERIM SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH 3 SCHEDULED THIRD INTERIM SACRIFICE T SCHEDULED TERMINAL SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH 3 SCHEDULED THIRD INTERIM SACRIFICE T SCHEDULED TERMINAL SACRIFICE T SCHEDULED TERMINAL SACRIFICE T SCHEDULED TERMINAL SACRIFICE 2 SCHEDULED SECOND INTERIM SACRIFICE 3 SCHEDULED THIRD INTERIM SACRIFICE D UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH D UNSCHEDULED UNSCHEDULED DEATH 2 SCHEDULED SECOND INTERIM SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH 2 SCHEDULED SECOND INTERIM SACRIFICE T SCHEDULED TERMINAL SACRIFICE T SCHEDULED TERMINAL SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH DATE OF DAY OF DEATH STUDY 10/24/99 04/20/99 04/20/00 11/02/99 01/24/00 07/21/98 04/21/00 04/21/00 12/14/99 02/14/00 04/21/00 11/01/99 07/21/98 10/24/99 04/20/99 04/21/00 12/01/99 04/20/00 12/20/99 04/20/99 04/20/00 04/19/00 04/19/00 07/21/98 04/20/99 09/05/99 10/22/99 12/27/99 07/21/98 03/21/00 07/21/98 04/19/00 04/21/00 01/25/00 553 366 732 562 645 93 733 733 604 666 733 561 93 553 366 733 591 732 610 366 732 731 731 93 366 504 551 617 93 702 93 731 733 646 WEEK OF STUDY 79 53 105 81 93 14 105 105 87 96 105 81 14 79 53 105 85 105 88 53 105 105 105 14 53 72 79 89 14 101 14 105 105 93 PAGE: 12 532 APPENDIX 2 Individual Animal Fate Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER C92909 C92910 C92911 C92912 C92913 C92914 C92915 C92916 C92917 C92918 C92919 C92920 C92921 C92922 C92923 C92924 C92925 C92926 C92927 C92928 C92929 C92930 C92931 C92932 C92933 C92934 C92935 C92936 C92937 C92938 C92939 C92940 C92941 C92942 DOSE GROUP 2222222222221111111111111111111111 SEX FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE DEATH CODE TYPE OF DEATH DESCRIPTION OF DEATH T SCHEDULED TERMINAL SACRIFICE 3 SCHEDULED THIRD INTERIM SACRIFICE T SCHEDULED TERMINAL SACRIFICE T SCHEDULED TERMINAL SACRIFICE 1T SCHEDULED TERMINAL SACRIFICE SCHEDULED FIRST INTERIM SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH 3 SCHEDULED THIRD INTERIM SACRIFICE 1M UNSCHEDULED UNSCHEDULED DEATH SCHEDULED FIRST INTERIM SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH 3 SCHEDULED THIRD INTERIM SACRIFICE 3 SCHEDULED THIRD INTERIM SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH 1T SCHEDULED TERMINAL SACRIFICE SCHEDULED FIRST INTERIM SACRIFICE T SCHEDULED TERMINAL SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH 3 SCHEDULED THIRD INTERIM SACRIFICE D UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH 1M UNSCHEDULED UNSCHEDULED DEATH SCHEDULED FIRST INTERIM SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH DATE OF DAY OF DEATH STUDY 04/20/00 04/20/99 04/20/00 04/20/00 04/19/00 05/15/98 02/18/00 01/28/00 04/20/99 09/09/99 05/15/98 08/30/99 05/17/99 04/20/99 04/20/99 11/29/99 04/21/00 05/15/98 04/19/00 11/15/99 06/08/99 04/20/99 08/22/99 0011//2291//0909 12/13/99 12/22/99 04/20/00 07/13/99 04/20/00 08/23/99 12/13/99 05/15/98 04/02/99 732 366 732 732 731 26 670 649 366 508 26 498 393 366 366 589 733 26 731 575 415 366 490 285 642 603 612 732 450 732 491 603 26 348 WEEK OF STUDY 105 53 105 105 105 4 96 93 53 73 4 72 57 53 53 85 105 4 105 83 60 53 70 41 92 87 88 105 65 105 71 87 4 50 PAGE: 13 533 APPENDIX 2 Individual Animal Fate Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER C92943 C92944 C92945 C92946 C92947 C92948 C92949 C92950 C92951 C92952 C92953 C92954 C92955 C92956 C92957 C92958 C92959 C92960 C92961 C92962 C92963 C92964 C92965 C92966 C92967 C92968 C92969 C92970 C92971 C92972 C92973 C92974 C92975 C92976 DOSE GROUP 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 SEX FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE DEATH CODE TYPE OF DEATH DESCRIPTION OF DEATH M UNSCHEDULED UNSCHEDULED DEATH 2 SCHEDULED SECOND INTERIM SACRIFICE 1 SCHEDULED FIRST INTERIM SACRIFICE T SCHEDULED TERMINAL SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH 1 SCHEDULED FIRST INTERIM SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH 2 SCHEDULED SECOND INTERIM SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE D UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH 2 SCHEDULED SECOND INTERIM SACRIFICE D UNSCHEDULED UNSCHEDULED DEATH 1 SCHEDULED FIRST INTERIM SACRIFICE T SCHEDULED TERMINAL SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE T SCHEDULED TERMINAL SACRIFICE T SCHEDULED TERMINAL SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH DATE OF DAY OF DEATH STUDY 02/25/00 07/21/98 05/15/98 04/20/00 02/04/00 03/10/00 12/04/98 05/15/98 04/03/00 07/19/99 04/21/00 03/23/00 01/17/00 03/28/00 04/21/00 12/29/99 02/28/00 04/21/00 03/10/00 07/21/98 02/04/00 04/19/00 11/13/99 09/03/99 07/21/98 08/09/99 05/15/98 04/19/00 01/03/00 02/18/00 04/19/00 04/20/00 04/21/00 03/31/00 677 93 26 732 656 691 229 26 715 456 733 704 638 709 733 619 680 733 691 93 656 731 573 502 93 477 26 731 624 670 731 732 733 712 WEEK OF STUDY 97 14 4 105 94 99 33 4 103 66 105 101 92 102 105 89 98 105 99 14 94 105 82 72 14 69 4 105 90 96 105 105 105 102 PAGE: 14 534 APPENDIX 2 Individual Animal Fate Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER C92977 C92978 C92979 C92980 C92981 C92982 C92983 C92984 C92985 C92986 C92987 C92988 C92989 C92990 C92991 C92992 C92993 C92994 C92995 C92996 C92997 C92998 C92999 C93000 C93001 C93002 C93003 C93004 C93005 C93006 C93007 C93008 C93009 C93010 DOSE GROUP 2 2 2 2 2 2 2 2 2 2 2 2 2 2 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 SEX FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE DEATH CODE TYPE OF DEATH DESCRIPTION OF DEATH D UNSCHEDULED UNSCHEDULED DEATH 2 SCHEDULED SECOND INTERIM SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH 1 SCHEDULED FIRST INTERIM SACRIFICE T SCHEDULED TERMINAL SACRIFICE T SCHEDULED TERMINAL SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE 2 SCHEDULED SECOND INTERIM SACRIFICE T SCHEDULED TERMINAL SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH 2 SCHEDULED SECOND INTERIM SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH 1 SCHEDULED FIRST INTERIM SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH D UNSCHEDULED UNSCHEDULED DEATH 2 SCHEDULED SECOND INTERIM SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH 1 SCHEDULED FIRST INTERIM SACRIFICE T SCHEDULED TERMINAL SACRIFICE 1 SCHEDULED FIRST INTERIM SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH DATE OF DEATH 03/05/00 07/21/98 0 1 / 01/00 06/28/99 09/03/99 05/15/98 04/19/00 04/20/00 02/28/00 04/21/00 07/21/98 04/19/00 11/22/99 02/01/00 04/07/00 11/23/99 07/21/98 11/12/99 06/17/98 05/15/98 11/15/99 03/01/99 0 1 /22/00 07/21/98 11/24/99 11/01/99 04/07/00 09/21/99 01/09/00 05/15/98 04/07/00 05/15/98 01/21/00 0 1 / 10/00 DAY OF STUDY 686 93 622 435 502 26 731 732 680 733 93 731 582 653 719 583 93 572 59 26 575 316 643 93 584 561 719 520 630 26 719 26 642 631 WEEK OF STUDY 98 14 89 63 72 4 105 105 98 105 14 105 84 94 103 84 14 82 9 4 83 46 92 14 84 81 103 75 90 4 103 4 92 91 PAGE: 15 535 APPENDIX 2 Individual Animal Fate Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL DOSE NUMBER GROUP SEX C93011 3 FEMALE C93012 3 FEMALE C93013 3 FEMALE C93014 3 FEMALE C93015 3 FEMALE C93016 3 FEMALE C93017 3 FEMALE C93018 3 FEMALE C93019 3 FEMALE C93020 3 FEMALE C93021 3 FEMALE C93022 3 FEMALE C93023 3 FEMALE C93024 3 FEMALE C93025 3 FEMALE C93026 3 FEMALE C93027 3 FEMALE C93028 3 FEMALE C93029 3 FEMALE C93030 3 FEMALE C93031 3 FEMALE C93032 3 FEMALE C93033 3 FEMALE C93034 3 FEMALE C93035 3 FEMALE C93036 3 FEMALE C93037 3 FEMALE C93038 3 FEMALE C93039 3 FEMALE C93040 3 FEMALE C93041 3 FEMALE C93042 3 FEMALE C93043 3 FEMALE C93044 3 FEMALE DEATH CODE TYPE OF DEATH DESCRIPTION OF DEATH T SCHEDULED TERMINAL SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH D UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH 2 SCHEDULED SECOND INTERIM SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH D UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE 2 SCHEDULED SECOND INTERIM SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH D UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH 1 SCHEDULED FIRST INTERIM SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH D UNSCHEDULED UNSCHEDULED DEATH 2 SCHEDULED SECOND INTERIM SACRIFICE D UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE T SCHEDULED TERMINAL SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH DATE OF DAY OF DEATH STUDY 04/07/00 11/01/99 12/06/99 10/01/99 03/31/00 11/11/99 0 1 / 10/00 07/21/98 02/22/99 07/24/99 06/28/99 04/07/00 07/21/98 12/08/99 03/23/00 04/03/00 0 1 / 10/00 01/17/00 09/01/99 02/18/00 05/15/98 10/27/99 07/19/99 11/15/99 07/21/98 12/02/99 07/22/99 08/23/99 04/07/00 04/07/00 11/15/99 02/13/00 02/21/00 07/19/99 719 561 596 530 712 571 631 93 309 461 435 719 93 598 704 715 631 638 500 670 26 556 456 575 93 592 459 491 719 719 575 665 673 456 WEEK OF STUDY 103 81 86 76 102 82 91 14 45 66 63 103 14 86 101 103 91 92 72 96 4 80 66 83 14 85 66 71 103 103 83 95 97 66 PAGE: 16 536 APPENDIX 2 Individual Animal Fate Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL DOSE NUMBER GROUP SEX C93045 3 FEMALE C93046 3 FEMALE C93047 3 FEMALE C93048 3 FEMALE C93049 3 FEMALE C93050 3 FEMALE C93051 4 FEMALE C93052 4 FEMALE C93053 4 FEMALE C93054 4 FEMALE C93055 4 FEMALE C93056 4 FEMALE C93057 4 FEMALE C93058 4 FEMALE C93059 4 FEMALE C93060 4 FEMALE C93061 4 FEMALE C93062 4 FEMALE C93063 4 FEMALE C93064 4 FEMALE C93065 4 FEMALE C93066 4 FEMALE C93067 4 FEMALE C93068 4 FEMALE C93069 4 FEMALE C93070 4 FEMALE C93071 4 FEMALE C93072 4 FEMALE C93073 4 FEMALE C93074 4 FEMALE C93075 4 FEMALE C93076 4 FEMALE C93077 4 FEMALE C93078 4 FEMALE DEATH CODE 1 M T T M M 2 M M M M M M 1 T T D T T 2 M M 1 D M 1 2 T D M 1 M T T TYPE OF DEATH SCHEDULED UNSCHEDULED SCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED SCHEDULED SCHEDULED UNSCHEDULED SCHEDULED SCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED SCHEDULED SCHEDULED UNSCHEDULED UNSCHEDULED SCHEDULED UNSCHEDULED SCHEDULED SCHEDULED DESCRIPTION OF DEATH FIRST INTERIM SACRIFICE UNSCHEDULED DEATH TERMINAL SACRIFICE TERMINAL SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH SECOND INTERIM SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH UNSCHEDULED DEATH FIRST INTERIM SACRIFICE TERMINAL SACRIFICE TERMINAL SACRIFICE UNSCHEDULED DEATH TERMINAL SACRIFICE TERMINAL SACRIFICE SECOND INTERIM SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH FIRST INTERIM SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH FIRST INTERIM SACRIFICE SECOND INTERIM SACRIFICE TERMINAL SACRIFICE UNSCHEDULED DEATH UNSCHEDULED DEATH FIRST INTERIM SACRIFICE UNSCHEDULED DEATH TERMINAL SACRIFICE TERMINAL SACRIFICE DATE OF DAY OF DEATH STUDY 05/15/98 10/25/99 04/07/00 04/07/00 08/16/99 03/02/00 07/21/98 09/01/99 07/22/99 09/05/99 10/04/99 03/10/00 03/10/00 05/15/98 04/20/00 04/21/00 10/29/99 04/19/00 04/19/00 07/21/98 01/24/00 01/24/00 05/15/98 04/06/00 03/21/00 05/15/98 07/21/98 04/20/00 09/23/99 11/15/99 05/15/98 02/01/00 04/21/00 04/19/00 26 554 719 719 484 683 93 500 459 504 533 691 691 26 732 733 558 731 731 93 645 645 26 718 702 26 93 732 522 575 26 653 733 731 WEEK OF STUDY 4 80 103 103 70 98 14 72 66 72 77 99 99 4 105 105 80 105 105 14 93 93 4 103 101 4 14 105 75 83 4 94 105 105 PAGE: 17 537 APPENDIX 2 Individual Animal Fate Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL DOSE NUMBER GROUP SEX C93079 4 FEMALE C93080 4 FEMALE C93081 4 FEMALE C93082 4 FEMALE C93083 4 FEMALE C93084 4 FEMALE C93085 4 FEMALE C93086 4 FEMALE C93087 4 FEMALE C93088 4 FEMALE C93089 4 FEMALE C93090 4 FEMALE C93091 4 FEMALE C93092 4 FEMALE C93093 4 FEMALE C93094 4 FEMALE C93095 4 FEMALE C93096 4 FEMALE C93097 4 FEMALE C93098 4 FEMALE C93099 4 FEMALE C93100 4 FEMALE C93101 4 FEMALE C93102 4 FEMALE C93103 4 FEMALE C93104 4 FEMALE C93105 4 FEMALE C93106 4 FEMALE C93107 4 FEMALE C93108 4 FEMALE C93109 4 FEMALE C93110 4 FEMALE C93111 5 FEMALE C93112 5 FEMALE DEATH CODE TYPE OF DEATH DESCRIPTION OF DEATH T SCHEDULED TERMINAL SACRIFICE T SCHEDULED TERMINAL SACRIFICE T SCHEDULED TERMINAL SACRIFICE D UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH 1 SCHEDULED FIRST INTERIM SACRIFICE 2 SCHEDULED SECOND INTERIM SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH D UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH D UNSCHEDULED UNSCHEDULED DEATH D UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH D UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE T SCHEDULED TERMINAL SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH D UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE D UNSCHEDULED UNSCHEDULED DEATH 2 SCHEDULED SECOND INTERIM SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH 2 SCHEDULED SECOND INTERIM SACRIFICE 3 SCHEDULED THIRD INTERIM SACRIFICE DATE OF DAY OF DEATH STUDY 04/20/00 04/21/00 04/20/00 02/25/00 04/17/00 05/15/98 07/21/98 01/09/00 03/16/00 10/15/99 02/04/00 04/19/00 10/06/99 10/06/99 02/18/00 02/18/00 04/17/00 11/22/98 02/28/00 04/20/00 02/19/00 12/28/99 04/20/00 04/21/00 03/10/00 11/23/99 12/28/99 02/09/00 04/19/00 04/18/99 07/21/98 01/09/00 07/21/98 04/20/99 732 733 732 677 729 26 93 630 697 544 656 731 535 535 670 670 729 217 680 732 671 618 732 733 691 583 618 661 731 364 93 630 93 366 WEEK OF STUDY 105 105 105 97 105 4 14 90 100 78 94 105 77 77 96 96 105 31 98 105 96 89 105 105 99 84 89 95 105 52 14 90 14 53 PAGE: 18 538 APPENDIX 2 Individual Animal Fate Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL DOSE NUMBER GROUP SEX C93113 5 FEMALE C93114 5 FEMALE C93115 5 FEMALE C93116 5 FEMALE C93117 5 FEMALE C93118 5 FEMALE C93119 5 FEMALE C93120 5 FEMALE C93121 5 FEMALE C93122 5 FEMALE C93123 5 FEMALE C93124 5 FEMALE C93125 5 FEMALE C93126 5 FEMALE C93127 5 FEMALE C93128 5 FEMALE C93129 5 FEMALE C93130 5 FEMALE C93131 5 FEMALE C93132 5 FEMALE C93133 5 FEMALE C93134 5 FEMALE C93135 5 FEMALE C93136 5 FEMALE C93137 5 FEMALE C93138 5 FEMALE C93139 5 FEMALE C93140 5 FEMALE C93141 5 FEMALE C93142 5 FEMALE C93143 5 FEMALE C93144 5 FEMALE C93145 5 FEMALE C93146 5 FEMALE DEATH CODE TYPE OF DEATH DESCRIPTION OF DEATH T SCHEDULED TERMINAL SACRIFICE 1 SCHEDULED FIRST INTERIM SACRIFICE T SCHEDULED TERMINAL SACRIFICE 3 SCHEDULED THIRD INTERIM SACRIFICE 3 SCHEDULED THIRD INTERIM SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH 3 SCHEDULED THIRD INTERIM SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH 1 SCHEDULED FIRST INTERIM SACRIFICE T SCHEDULED TERMINAL SACRIFICE T SCHEDULED TERMINAL SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH 2 SCHEDULED SECOND INTERIM SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH D UNSCHEDULED UNSCHEDULED DEATH 3 SCHEDULED THIRD INTERIM SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE D UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE D UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE 3 SCHEDULED THIRD INTERIM SACRIFICE T SCHEDULED TERMINAL SACRIFICE T SCHEDULED TERMINAL SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH 1 SCHEDULED FIRST INTERIM SACRIFICE 2 SCHEDULED SECOND INTERIM SACRIFICE 1 SCHEDULED FIRST INTERIM SACRIFICE D UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE DATE OF DAY OF DEATH STUDY 04/19/00 05/15/98 04/20/00 04/20/99 04/20/99 01/24/00 01/17/00 04/20/99 10/20/99 01/21/00 05/15/98 04/20/00 04/19/00 05/30/99 07/21/98 03/13/00 03/13/00 04/20/99 02/14/00 04/17/00 04/21/00 08/05/99 04/19/00 05/09/99 04/20/00 04/20/99 04/21/00 04/20/00 03/17/00 05/15/98 07/21/98 05/15/98 09/24/99 04/20/00 731 26 732 366 366 645 638 366 549 642 26 732 731 406 93 694 694 366 666 729 733 473 731 385 732 366 733 732 698 26 93 26 523 732 WEEK OF STUDY 105 4 105 53 53 93 92 53 79 92 4 105 105 58 14 100 100 53 96 105 105 68 105 55 105 53 105 105 100 4 14 4 75 105 PAGE: 19 539 APPENDIX 2 Individual Animal Fate Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL DOSE NUMBER GROUP SEX C93147 5 FEMALE C93148 5 FEMALE C93149 5 FEMALE C93150 5 FEMALE C93151 5 FEMALE C93152 5 FEMALE C93153 5 FEMALE C93154 5 FEMALE C93155 5 FEMALE C93156 5 FEMALE C93157 5 FEMALE C93158 5 FEMALE C93159 5 FEMALE C93160 5 FEMALE C93161 5 FEMALE C93162 5 FEMALE C93163 5 FEMALE C93164 5 FEMALE C93165 5 FEMALE C93166 5 FEMALE C93167 5 FEMALE C93168 5 FEMALE C93169 5 FEMALE C93170 5 FEMALE C93171 5 FEMALE C93172 5 FEMALE C93173 5 FEMALE C93174 5 FEMALE C93175 5 FEMALE C93176 5 FEMALE C93177 5 FEMALE C93178 5 FEMALE C93179 5 FEMALE C93180 5 FEMALE DEATH CODE TYPE OF DEATH DESCRIPTION OF DEATH 3 SCHEDULED THIRD INTERIM SACRIFICE D UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE 3 SCHEDULED THIRD INTERIM SACRIFICE 2 SCHEDULED SECOND INTERIM SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE T SCHEDULED TERMINAL SACRIFICE 1 SCHEDULED FIRST INTERIM SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE T SCHEDULED TERMINAL SACRIFICE 3 SCHEDULED THIRD INTERIM SACRIFICE T SCHEDULED TERMINAL SACRIFICE T SCHEDULED TERMINAL SACRIFICE T SCHEDULED TERMINAL SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE 2 SCHEDULED SECOND INTERIM SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH 3 SCHEDULED THIRD INTERIM SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE T SCHEDULED TERMINAL SACRIFICE T SCHEDULED TERMINAL SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH DATE OF DAY OF DEATH STUDY 04/20/99 01/15/00 04/19/00 01/19/00 04/21/00 07/19/99 04/20/00 04/20/99 07/21/98 12/06/99 04/21/00 04/19/00 05/15/98 09/27/99 04/19/00 04/19/00 04/20/99 04/19/00 04/21/00 04/20/00 01/12/99 04/20/00 07/21/98 02/01/00 04/20/99 10/17/99 03/28/00 04/19/00 0 1 /20/00 12/23/99 04/21/00 04/21/00 04/21/00 06/28/99 366 636 731 640 733 456 732 366 93 596 733 731 26 526 731 731 366 731 733 732 268 732 93 653 366 546 709 731 641 613 733 733 733 435 WEEK OF STUDY 53 91 105 92 105 66 105 53 14 86 105 105 4 76 105 105 53 105 105 105 39 105 14 94 53 78 102 105 92 88 105 105 105 63 PAGE: 20 540 APPENDIX 2 Individual Animal Fate Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER C93181 C93182 C93183 C93184 C93185 C93186 C93187 C93188 C93189 C93190 C93191 C93192 C93193 C93194 C93195 C93196 C93197 C93198 C93199 C93200 C93201 C93202 C93203 C93204 C93205 C93206 C93207 C93208 C93209 C93210 C93211 C93212 C93213 C93214 DOSE GROUP 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 SEX FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE DEATH CODE TYPE OF DEATH DESCRIPTION OF DEATH U SCHEDULED FIRST POST-RECOV SAC U SCHEDULED FIRST POST-RECOV SAC U SCHEDULED FIRST POST-RECOV SAC U SCHEDULED FIRST POST-RECOV SAC U SCHEDULED FIRST POST-RECOV SAC M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH D UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH U SCHEDULED FIRST POST-RECOV SAC M UNSCHEDULED UNSCHEDULED DEATH U SCHEDULED FIRST POST-RECOV SAC M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH D UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH U SCHEDULED FIRST POST-RECOV SAC U SCHEDULED FIRST POST-RECOV SAC M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH U SCHEDULED FIRST POST-RECOV SAC U SCHEDULED FIRST POST-RECOV SAC M UNSCHEDULED UNSCHEDULED DEATH D UNSCHEDULED UNSCHEDULED DEATH U SCHEDULED FIRST POST-RECOV SAC M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH D UNSCHEDULED UNSCHEDULED DEATH U SCHEDULED FIRST POST-RECOV SAC M UNSCHEDULED UNSCHEDULED DEATH U SCHEDULED FIRST POST-RECOV SAC D UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH D UNSCHEDULED UNSCHEDULED DEATH DATE OF DAY OF DEATH STUDY 04/24/00 04/24/00 04/24/00 04/24/00 04/24/00 09/21/99 04/13/00 04/22/00 12/15/99 04/24/00 01/12/00 04/24/00 10/01/99 01/17/00 08/23/99 12/24/99 04/24/00 04/24/00 07/13/99 02/19/00 04/24/00 04/24/00 01/03/00 01/21/00 04/24/00 04/15/00 01/25/99 11/16/99 04/24/00 04/22/00 04/24/00 11/24/99 11/26/99 03/03/00 736 736 736 736 736 520 725 734 605 736 633 736 530 638 491 614 736 736 450 671 736 736 624 642 736 727 281 576 736 734 736 584 586 684 WEEK OF STUDY 106 106 106 106 106 75 104 105 87 106 91 106 76 92 71 88 106 106 65 96 106 106 90 92 106 104 41 83 106 105 106 84 84 98 PAGE: 21 541 APPENDIX 2 Individual Animal Fate Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER C93215 C93216 C93217 C93218 C93219 C93220 DOSE GROUP 6 6 6 6 6 6 SEX FEMALE FEMALE FEMALE FEMALE FEMALE FEMALE DEATH CODE TYPE OF DEATH DESCRIPTION OF DEATH M UNSCHEDULED UNSCHEDULED DEATH U SCHEDULED FIRST POST-RECOV SAC D UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH U SCHEDULED FIRST POST-RECOV SAC U SCHEDULED FIRST POST-RECOV SAC DATE OF DAY OF DEATH STUDY 12/06/99 04/24/00 01/14/00 01/03/00 04/24/00 04/24/00 596 736 635 624 736 736 WEEK OF STUDY 86 106 91 90 106 106 PAGE: 22 542 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 1 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: Ml DOSE: 0 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92501 3 53 NORMAL NO REMARKABLE OBSERVATIONS M 77 APPEARANCE THIN BEHAVIOR ATAXIC <UNSPECIFIED> HYPOACTIVE <UNSPECIFIED> DISCHARGE GENITAL RED IN COLOR EYES RED DISCHARGE EYE-LEFT NORMAL NO REMARKABLE OBSERVATIONS 1 , 8 ,r 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365- 366 533 533 533 274, 281, 337 533 1 , 8 ,r 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225 , 232, 239, 246, 253 , 260, 267, 288, 295 , 302 309, 316, 323, 330 , 344, 351, 358, 365 , 372, 379, 386, 393 , 400 407, 414, 421, 428 , 435, 442, 449, 456 , 463, 470, 477, 484 , 491 498, 505, 512, 519 , 526 543 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 2 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: Ml DOSE: 0 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92503 T 105 APPEARANCE THIN RESPIRATION AUDIBLE NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) TAIL-PROXIMAL WEEK 72 C92504 3 WEEK 105 53 APPEARANCE MALOCCLUSION MISSING TEETH NORMAL NO REMARKABLE OBSERVATIONS 715, 722, 72 9, 733 575, 582, 589, 596 1 , 8 ,. 15, 2 2 , 29, 36, 43, 50, 57, 64, 71, 78, 85 , 92, 99, 106 113, 1 2 0, 127,. 134, 141, 148, 155, 162, 169, 176 , 183, 190 , 197 204, 2 1 1 , 218,. 225, 232, 239, 246, 253, 260, 267 , 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344, 351, 358 , 365, 372 , 379 386, 393, 400,. 407, 414, 421, 428, 435, 442, 449 , 456, 463 , 470 477, 484, 491 498C, 575C, 652C, 72 9C, 729C, 505C , 512C, 582C , 589C, 659C , 666C, 733C 733C 519C, 596C, 673C, 526C, 603C, 680C, 533C , 540C, 610C , 617C, 687C , 694C, 547C, 624C, 701C, 554C, 631C, 708C, 561C , 568C 638C , 645C 715C , 722C 78, 85, 92 , 99, 106,r 113,, 1 2 0, 127,, 134, 141,, 148,, 155,, 162, 169 176,. 183, 190, 197, 204, 2 1 1 , 218, 225, 232, 239, 246, 253, 260 267,. 274, 281, 288, 295, 302, 309, 316, 323, 330, 337, 344, 351 358, 365-366 78 1 , 8 15, 22, 29, 36, 43, 50, 57, 64 71 544 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 3 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: Ml (CONTINUED FROM PREVIOUS PAGE) C92504 3 53 SURGERY PERFORMED BY KATHY EWERS ANTIBIOTIC ADMINISTERED 0.5 ML C92505 T 105 APPEARANCE MALOCCLUSION EYES SQUINTED PERI-ORBITAL NORMAL NO REMARKABLE OBSERVATIONS DOSE: 0 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 359 359-360 673, 680, 687,. 694 , 701, 708, 715 512, 519, 526, 533 , 540, 547, 554, 561 , 568, 575, 582, 589 , 596 603, 610 1 , 8 ,r 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407 , 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498 , 505, 617, 624, 631 , 638, 645, 652, 659 , 666 722, 729, 731 545 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 4 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: Ml DOSE: 0 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92506 1 C92507 3 4 NORMAL NO REMARKABLE OBSERVATIONS 53 NORMAL NO REMARKABLE OBSERVATIONS C92508 D 77 SKIN & PELAGE ROUGH HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS C92509 2 C92510 T 14 NORMAL NO REMARKABLE OBSERVATIONS 105 APPEARANCE MALOCCLUSION NORMAL NO REMARKABLE OBSERVATIONS 1, 8,, 15, 22 1 22, 8,, 15, , 29, 36, 43,, 50, 57, 64, 71, 78, 85, 92, 99, 106 120113, , 127,. 134, 141, 148, 155,. 162, 169, 176, 183 , 190 , 197 211204, , 218,. 225, 232, 239, 246,. 253, 260, 267, 274 , 281 , 288 295, 302, 309,. 316, 323, 330, 337,. 344, 351, 358, 365 -366 533, 537 1 22, 8,, 15, , 29, 36, 43,, 50, 57, 64, 71, 78, 85, 92, 99, 106 120113, , 127,. 134, 141, 148, 155,. 162, 169, 176, 183 , 190 , 197 211204, , 218,. 225, 232, 239, 246,. 253, 260, 267, 274 , 281 , 288 295, 302, 309,. 316, 323, 330, 337,. 344, 351, 358, 365 , 372 , 379 386, 393, 400,. 407, 414, 421, 428,. 435, 442, 449, 456 , 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 1 22, 8,, 15, , 29, 36, 43,, 50, 57, 64, 71, 78, 85, 92- 93 470, 477, 484,. 512, 519, 526, 533,. 561, 610, 617, 645 , 652 1 22, 8,, 15, , 29, 36, 43,, 50, 57, 64, 71, 78, 85, 92, 99, 106 120113, , 127,. 134, 141, 148, 155,. 162, 169, 176, 183 , 190 , 197 211204, , 218,. 225, 232, 239, 246,. 253, 260, 267, 274 , 281 , 288 295, 302, 309,. 316, 323, 330, 337,. 344, 351, 358, 365 , 372 , 379 386, 393, 400,. 407, 414, 421, 428,. 435, 442, 449, 456 , 463 , 491 546 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 5 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: Ml (CONTINUED FROM PREVIOUS PAGE) C92510 T 105 NORMAL NO REMARKABLE OBSERVATIONS C92511 2 C92512 3 14 NORMAL NO REMARKABLE OBSERVATIONS 53 NORMAL NO REMARKABLE OBSERVATIONS C92513 T SURGERY PERFORMED BY KATHY EWERS ANTIBIOTIC ADMINISTERED 0.5 ML 105 SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE NORMAL NO REMARKABLE OBSERVATIONS DOSE: 0 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 498, 505, 54 0,, 547, 554, 568, 575, 582 , 589, 596, 603 , 624 , 631 638, 659, 666, 673, 680, 687, 694, 701 , 708, 715, 722 , 729 , 732 1 , 8 , 15, 2 2 , 29, 36, 43,, 50, 57, 64, 71, 78, 85, 92- 93 1 , 8 , 15, 2 2 , 29, 36, 43,, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127,, 134, 141, 148, 155, 162 , 169, 176, 183 , 190 , 197 204, 2 1 1 , 218,, 225, 232, 239, 246, 253 , 260, 267, 274 , 281 , 288 295, 302, 309,, 316, 323, 330, 337, 344 , 351, 358, 365 -366 359 359- 360 428, 435, 442 1 , 8 , 15, 2 2 , 29, 36, 43,, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127,, 134, 141, 148, 155, 162 , 169, 176, 183 , 190 , 197 204, 2 1 1 , 218,, 225, 232, 239, 246, 253 , 260, 267, 274 , 281 , 288 295, 302, 309,, 316, 323, 330, 337, 344 , 351, 358, 365 , 372 , 379 386, 393, 400,, 407, 414, 421, 449, 456 , 463, 470, 477 , 484 , 491 498, 505, 512,, 519, 526, 533, 540, 547 , 554, 561, 568 , 575 , 582 547 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 6 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: Ml (CONTINUED FROM PREVIOUS PAGE) C92513 T 105 NORMAL NO REMARKABLE OBSERVATIONS C92514 T 105 APPEARANCE MALOCCLUSION SWOLLEN PAW-HIND-RIGHT SKIN & PELAGE SORE/SCAB PAW-HIND-RIGHT NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) TAIL-PROXIMAL WEEK 102 DOSE: 0 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 589, 596, 603, 610, 617, 624, 631, 638, 645, 652, 659, 666, 673 680, 687, 694, 701, 708, 715, 722, 729, 732 414, 421 617, 624, 631, 638, 645, 652 708 1 , 8 , 15 113, 120 204, 211 295, 302 386, 393 491, 498 582, 589 733 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 400, 407 , 428, 435, 442, 449 , 456, 463, 470, 477 , 484 505, 512 , 519, 526, 533, 540 , 547, 554, 561, 568 , 575 596, 603 , 610, 659, 666, 673 , 680, 687, 694, 701 , 729 708, 715, 722 548 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 7 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: Ml DOSE: 0 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92515 D 99 APPEARANCE MALOCCLUSION EYES SQUINTED PERI-ORBITAL NORMAL NO REMARKABLE OBSERVATIONS C92516 D 47 NORMAL NO REMARKABLE OBSERVATIONS C92517 3 53 NORMAL NO REMARKABLE OBSERVATIONS 512, 519, 526, 533, 540 547, 554 561 603, 610 1 , 8 , 15, 2 2 , 29, 36 , 43,, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190, 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281, 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463, 470 477, 484, 491, 498, 505, 568, 575, 582 , 589, 596, 617, 624, 631 638, 645, 652, 659, 666, 673, 680, 687 , 693 1 , 8 , 15, 2 2 , 29, 36 , 43,, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190, 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281, 288 295, 302, 309,. 316, 323, 326 1 , 8 , 15, 2 2 , 29, 36 , 43,, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190, 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281, 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365- 366 549 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 8 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: Ml DOSE: 0 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92518 T 105 APPEARANCE SWOLLEN PAW-HIND-LEFT NORMAL NO REMARKABLE OBSERVATIONS C92519 D 80 NORMAL NO REMARKABLE OBSERVATIONS C92520 D 83 APPEARANCE LIMITED USE LIMB-HIND-RIGHT PAW-HIND-RIGHT SWOLLEN DIGIT(S)-HIND-RIGHT LIMB-HIND-RIGHT PAW-HIND-RIGHT 651- 652, 659, 666, 673, 680, 687 1 , 8 , 15, 2 2 , 29, 36, 43 , 50, 113, 1 2 0 , 127, 134 , 141, 148, 204, 2 1 1 , 218, 225 , 232, 239, 295, 302, 309, 316 , 323, 330, 386, 393, 400, 407 , 414, 421, 477, 484, 491, 498 , 505, 512, 568, 575, 582, 589 , 596, 603, 701, 708, 715, 722 , 729, 731 57, 64, 71, 78, 85, 155, 162 , 169, 176, 246, 253 , 260, 267, 337, 344 , 351, 358, 428, 435 , 442, 449, 519, 526 , 533, 540, 610, 617 , 624, 631, 92, 99, 106 183, 190 , 197 274, 281 , 288 365, 372 , 379 456, 463 , 470 547, 554 , 561 638, 645 , 694 1 , 8 ,r 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407 , 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498 , 505, 512, 519, 526 , 533, 540, 547, 554 , 557 491, 498, 505, 512, 519, 526, 533, 540, 547, 554, 561, 568, 575 578 470, 477 533, 540, 547, 554, 561, 568, 575, 578 5C 5C 4A, 5cr6i1, 5c6tz8o 554, 561, 568 550 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 9 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: Ml (CONTINUED FROM PREVIOUS PAGE) C92520 D 83 NORMAL NO REMARKABLE OBSERVATIONS C92521 2 C92522 M 14 NORMAL NO REMARKABLE OBSERVATIONS 98 BEHAVIOR HYPOACTIVE <UNSPECIFIED> RECUMBENT LATERAL EXCRETION FEW FECES EYES RED DISCHARGE EYES SQUINTED PERI-ORBITAL SKIN & PELAGE SORE/SCAB PAW-HIND-LEFT PAWS-HIND VENTRAL-ABDOMINAL DOSE: 0 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 1 , 8 ,r 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407 , 414, 421, 428, 435 , 442, 449, 456, 463 , 484 1, 8 , 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92-93 681 681 681 681 681 624, 631 638, 645, 652, 659, 666, 673, 680-681 330, 337, 344, 351, 449, 456, 463, 519, 526, 533, 631, 680-681 551 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 10 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: Ml (CONTINUED FROM PREVIOUS PAGE) C92522 M 98 NORMAL NO REMARKABLE OBSERVATIONS C92523 3 MASS(ES) VENTRAL-ABDOMINAL WEEK 63 WEEK 87 WEEK 97 VENTRAL -ABDOMINAL-LEFT WEEK 44 53 NORMAL NO REMARKABLE OBSERVATIONS C92524 1 4 NORMAL NO REMARKABLE OBSERVATIONS DOSE: 0 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 1 , 8 ,r 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 358, 365, 372 , 379, 386, 393, 400 , 407, 414, 421, 428 , 470 477, 484, 491, 498 , 505, 512, 540, 547 , 554, 561, 568, 575 , 582 589, 596 435C, 442C 603C, 610C, 617C, 624C 673 302C, 309C, 316C, 323C 1 , 8 ,r 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190, 197 204, 2 1 1 , 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281, 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365-366 1, 8 , 15, 22 552 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 11 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: Ml DOSE: 0 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92525 M 72 APPEARANCE THIN BEHAVIOR HYPOACTIVE MODERATE EXCRETION FEW FECES NO FECES SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE ROUGH HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) VENTRAL-ABDOMINAL WEEK 56 WEEK 61 428, 435, 442, 449, 456, 463, 470, 477, 484, 491, 498 498 428, 435, 470 498 421, 428, 435, 442 , 449, 456, 463 449, 456, 463, 470 , 477, 484, 491, 498 1 , 8 ,r 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190, 197 204, 2 1 1 , 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281, 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372, 379 393, 400, 407, 414 386 421, 428, 435, 442 , 449, 456, 463, 470 , 477C, 484C , 491C, 498C 553 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 12 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: Ml DOSE: 0 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92526 1 C92527 M 4 NORMAL NO REMARKABLE OBSERVATIONS 84 APPEARANCE HEAD TILT RIGHT LIMITED USE LIMB-HIND-RIGHT THIN BEHAVIOR ATAXIC <UNSPECIFIED> HYPOACTIVE <UNSPECIFIED> EXCRETION FEW FECES SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE SORE/SCAB PAW-HIND-LEFT NORMAL NO REMARKABLE OBSERVATIONS 1, 8 , 15, 22 575, 582, 584 584 582, 584 584 584 584 561, 568 575, 582 372, 379 386 , 393 , 400, 407, 414 1 , 8 , 15 113, 120 204, 211 295, 302 435, 442 526, 533 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 421 , 428 449, 456 , 463, 470, 477, 484 , 491, 498, 505, 512 , 519 540, 547 , 554 554 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 13 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: Ml DOSE: 0 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92528 2 C92529 1 C92530 M 14 NORMAL NO REMARKABLE OBSERVATIONS 4 NORMAL NO REMARKABLE OBSERVATIONS 79 BEHAVIOR RECUMBENT LATERAL EXCRETION FEW FECES RESPIRATION AUDIBLE IRREGULAR LABORED SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE NORMAL NO REMARKABLE OBSERVATIONS 1, 8 , 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92-93 1, 8 , 15, 22 547 547 547 547 547 1, 8 , 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400 407 , 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491 498 , 505, 512, 519, 526 , 533, 540 555 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 14 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: Ml DOSE: 0 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92531 M 68 BEHAVIOR RECUMBENT LATERAL EXCRETION FEW FECES NO FECES EYES RED DISCHARGE EYES SQUINTED PERI-ORBITAL RESPIRATION IRREGULAR NORMAL NO REMARKABLE OBSERVATIONS C92532 2 14 NORMAL NO REMARKABLE OBSERVATIONS 471 469-470 471 471 471 471 1 , 8 ,r 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407 , 414, 421, 428, 435 , 442, 449, 456, 463 1, 8 , 15, 22, 29, 36, 43, 50, 57, 64 71, 78 85 92-93 556 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 15 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: Ml DOSE: 0 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92533 M 85 SKIN & PELAGE RED SKIN HEAD-MAXILLARY-RIGHT SORE/SCAB HEAD-MAXILLARY-RIGHT NORMAL NO REMARKABLE OBSERVATIONS C92534 3 MASS(ES) HEAD-MAXILLARY-RIGHT WEEK 71 INGUINAL REGION-LEFT WEEK 69 53 APPEARANCE MALOCCLUSION NORMAL NO REMARKABLE OBSERVATIONS 456 561, 568, 575,. 582, 589 1 , 8 , 15, 2 2 , 29, 36, 43 , 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190, 197 204, 2 1 1 , 218,. 225, 232, 239, 246, 253 , 260, 267, 274, 281, 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372, 379 386, 393, 400,. 407, 414, 421, 428, 435 , 442, 449, 463, 470 491, 498, 505, 512C, 519C, 526C, 533C, 54 0C, 547C , 554C, 561C 568C, 575C , 582C, 589C 477, 484, 491, 498, 505, 512C, 519C, 526C, 533C, 54 0C, 547C 554C, 561C , 568C, 575C, 582C, 589C 365-366 1 , 8 ,r 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127, 134 , 141, 148, 155, 162 , 169, 176, 183,. 190, 197 204, 2 1 1 , 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281, 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358 557 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 16 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: Ml (CONTINUED FROM PREVIOUS PAGE) C92534 3 53 SURGERY PERFORMED BY JOHN VAN BERKUM ANTIBIOTIC ADMINISTERED 0.5 ML C92535 M 96 APPEARANCE MALOCCLUSION TREMORS LIMBS-ALL BEHAVIOR HYPOACTIVE <UNSPECIFIED> COLD TO TOUCH ENTIRE BODY DISCHARGE GENITAL RED IN COLOR EXCRETION DISCOLORED FECES RED IN COLOR NONFORMED FECES EYES RED DISCHARGE EYES DOSE: 0 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 359 359-360 561 666 666 666 43 666 589, 666 302 558 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 17 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: Ml (CONTINUED FROM PREVIOUS PAGE) C92535 M 96 EYES SQUINTED PERI-ORBITAL NORMAL NO REMARKABLE OBSERVATIONS C92536 M SACRIFICE SACRIFICE APPROVED BY TOXICOLOGIST 78 APPEARANCE SWOLLEN NOSE BEHAVIOR COLD TO TOUCH ENTIRE BODY DISCHARGE GENITAL RED IN COLOR RESPIRATION AUDIBLE IRREGULAR DOSE: 0 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 666 1 22, 8,r 15, , 29, 36 , 50,r 57, 64, 71, 78 , 85,r 92, 99, 106, 113 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197, 204 211, 218, 225,. 232, 239, 246, 253, 260, 267, 274, 281, 288, 295 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379, 386, 393 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470, 477, 484 491, 498, 505,. 512, 519, 526, 533, 540, 547, 554, 568, 575, 582 596, 603, 610, 617, 624, 631, 638, 645, 652, 659 666 546 546 337 546 546 559 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 18 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: Ml (CONTINUED FROM PREVIOUS PAGE) C92536 M 78 NORMAL NO REMARKABLE OBSERVATIONS C92537 D 96 EXCRETION FEW FECES SKIN & PELAGE SORE/SCAB DORSAL NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) DORSAL WEEK 93 VENTRAL-CERVICAL-LEFT WEEK 91 DOSE: 0 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 1, 8 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 344, 351 , 358, 365, 372, 379 , 386 393, 400, 407, 414 , 421, 428, 435, 442 , 449, 456, 463, 470 , 477 484, 491, 498, 505 , 512, 519, 526, 533 , 540 662, 666 645, 652, 659 662, 666 1 , 8 ,r 15, 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582 589, 596, 603, 610, 617 , 624 645C, 652C, 659C, 662C, 666C 631C, 638C, 645C, 652C, 659C, 662C, 666C 560 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 19 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: Ml DOSE: 0 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92538 D 74 APPEARANCE OBES DISCHARGE GENITAL RED IN COLOR NORMAL NO REMARKABLE OBSERVATIONS C92539 M 98 APPEARANCE THIN EXCRETION FEW FECES EYES RED DISCHARGE EYE-LEFT EYES NORMAL NO REMARKABLE OBSERVATIONS 470, 477, 484, 491 , 498, 505, 512- 513 477 1 , 8 ,r 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407 , 414, 421, 428, 435 , 442, 449, 456, 463 673, 680 673, 680 617, 624 631, 638, 645, 652, 659 666, 673 680 1, 8 , 15 113, 120 204, 211 295, 302 386, 393 477, 484 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 400, 407 , 414, 421, 428, 435 , 442, 449, 456, 463 , 470 491, 498 , 505, 512, 519, 526 , 533, 540, 547, 554 , 561 561 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 20 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: Ml (CONTINUED FROM PREVIOUS PAGE) C92539 M 98 NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) TAIL-PROXIMAL WEEK 87 C92540 3 53 APPEARANCE MALOCCLUSION NORMAL NO REMARKABLE OBSERVATIONS C92541 T 105 NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) DORSAL WEEK 68 DOSE: 0 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 568, 575, 582, 589, 596 603, 610, 617, 624, 631, 638, 645, 652, 659, 666, 673, 680 197, 204 1 , 8 ,r 15, 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127,. 134, 141, 148, 155, 162 , 169, 176, 183,. 190, 211 218, 225, 232, 239, 246, 253, 260, 267 , 274, 281, 288, 295, 302 309, 316, 323, 330, 337, 344, 351, 358 , 365-366 1 , 8 ,. 15, 2 2 , 29, 36, 43 , 50, 57, 64, 71, 78, 85 , 92, 99, 106 113, 1 2 0 , 127,. 134, 141, 148, 155, 162, 169, 176 , 183, 190 , 197 204, 2 1 1 , 218,. 225, 232, 239, 246, 253, 260, 267 , 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344, 351, 358 , 365, 372 , 379 386, 393, 400,. 407, 414, 421, 428, 435, 442, 449 , 456, 463 , 680 687, 694, 701,. 708, 715, 722, 729, 732 470C, 477C , 484C, 491C, 498C, 505C , 512C, 519C, 526C, 533C , 540C 54 7C, 554C , 561C, 568C, 575C, 582C , 589C, 596C, 603C, 610C , 617C 624C, 631C , 638C, 645C, 652C, 659C , 666C, 673C 562 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 21 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: Ml (CONTINUED FROM PREVIOUS PAGE) C92541 T 105 WART LIKE LESION(S) NOSE WEEK 69 C92542 T 105 NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) AXILLARY REGION-LEFT WEEK 100 DORSAL WEEK 103 TAIL-PROXIMAL WEEK 93 WART LIKE LESION(S) DORSAL-CERVICAL-RIGHT WEEK 69 HEAD-MAXILLARY-RIGHT WEEK 66 DOSE: 0 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 477, 484 1 , 8 ,. 15, 2 2 , 29, 36, 43 , 50, 57, 64, 71 , 78, 85 , 92, 99, 106 113, 1 2 0 , 127, 134 , 141, 148, 155, 162, 169, 176 , 183, 190 , 197 204, 2 1 1 , 218, 225 , 232, 239, 246, 253, 260, 267 , 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344, 351, 358 , 365, 372 , 379 386, 393, 400, 407 , 414, 421, 428, 435, 442, 449 694, 701, 708 715C, 722C', 72 9C, 733C 645C, 652C , 659C, 666C, 673C, 680C , 687C', 694C, 701C, 708C , 715C 722C,. 72 9C , 733C 477, 484, 491, 498 , 505, 512, 519, 526, 533, 540 , 547, 554 , 561 568, 575, 582, 589 , 596, 603, 610, 617, 624, 631 , 638, 645 , 652 659, 666, 673, 680 , 687, 694, 701, 708, 715, 722 , 729, 733 456, 463, 470, 477 , 484, 491, 498, 505, 512, 519 , 526, 533 , 540 547, 554, 561, 568 , 575, 582, 589, 596, 603, 610 , 617, 624 , 631 638, 645, 652, 659 , 666, 673, 680, 687, 694, 701 , 708, 715 , 722 729, 733 563 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 22 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: Ml DOSE: 0 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92543 D 100 NORMAL NO REMARKABLE OBSERVATIONS T 105 APPEARANCE MALOCCLUSION MISSING TEETH SWOLLEN PAW-HIND-RIGHT PAWS-HIND TAIL THIN EXCRETION FEW FECES SKIN & PELAGE ROUGH HAIR COAT 1 , 8 , 15 , 2 2 , 29, 36 , 43 , 50 , 57, 64, 71, 78, 85, 92, 99, 10 6 113, 120 , 127, 134, 141, 148 , 155, 162 , 169, 176, 183, 190, 197 204, 211 , 218, 225, 232, 239 , 246, 253 , 260, 267, 274, 281, 288 295, 302 , 309, 316, 323, 330 , 337, 344 , 351, 358, 365, 372, 379 386, 393 , 400, 407, 414, 421 , 428, 435 , 442, 449, 456, 463, 470 477, 484 , 491, 498, 505, 512 , 519, 526 , 533, 540, 547, 554, 561 568, 575 , 582, 589, 596, 603 , 610, 617 , 624, 631, 638, 645, 652 659, 666 , 673, 680, 687, 694 -695 43, 50, 57, 59 , 64, 141, 148 , 155, 162, 232, 239 , 246, 253, 323, 330 , 337, 344, 414, 421 , 428, 435, 505, 512 , 519, 526, 596, 603 , 610, 617, 687, 694 , 701, 708, 71, 78, 85, 92 , 99 , 106, 169, 176 , 183, 190 , 197, 260, 267 , 274, 281 , 288, 351, 358 , 365, 372 , 379, 442, 449 , 456, 463 , 470, 533, 540 , 547, 554 , 561, 624, 631 , 638, 645 , 652, 715, 722 , 729, 732 113, 204, 295, 386, 477, 568, 659, 12 0 , 127, 2 1 1 , 218, 302, 309, 393, 400, 484, 491, 575, 582, 666, 673, 134 225 316 407 498 589 680 708, 715 , 722, 729, 732 666, 673 680, 687 , 694, 729, 732 59, 435 701, 708, 715 , 722, 729 , 732 59, 435 59 564 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 23 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: Ml (CONTINUED FROM PREVIOUS PAGE) C92544 T 105 SKIN & PELAGE SORE/SCAB TAIL-PROXIMAL NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) LIMB-FRONT-LEFT WEEK 102 WART LIKE LESION(S) PAW-HIND-LEFT WEEK 95 C92545 D 94 NORMAL NO REMARKABLE OBSERVATIONS WART LIKE LESION(S) DORSAL WEEK 69 DOSE: 0 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 589, 596, 603, 610, 617, 624, 631 1, 8 15, 22, 29, 36 708C 715C, 722C, 729C, 732C 659, 666, 673, 680, 687, 694, 701, 708 , 715, 722, 729, 732 1, 8 15, 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 540, 547, 554, 561, 568, 575, 582, 589 , 596, 603, 610, 617 , 624 631, 638, 645, 652 477, 484, 491, 498, 505, 512, 519, 526 , 533 565 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 24 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: Ml DOSE: 0 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92546 1 C92547 M 4 NORMAL NO REMARKABLE OBSERVATIONS 88 APPEARANCE HEAD TILT RIGHT THIN BEHAVIOR ATAXIC <UNSPECIFIED> HYPOACTIVE <UNSPECIFIED> DISCHARGE GENITAL RED IN COLOR EXCRETION NO FECES EYES CLEAR DISCHARGE EYE-LEFT SKIN & PELAGE ROUGH HAIR COAT SORE/SCAB DORSAL YELLOW HAIR COAT 1, 8 , 15, 22 611 610-611 611 611 449 611 15 610-611 554, 561, 568, 575, 582, 589, 596, 603, 610 611 566 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 25 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: Ml (CONTINUED FROM PREVIOUS PAGE) C92547 M 88 NORMAL NO REMARKABLE OBSERVATIONS C92548 D 84 SKIN & PELAGE SORE/SCAB SHOULDER-RIGHT NORMAL NO REMARKABLE OBSERVATIONS C92549 D 80 NORMAL NO REMARKABLE OBSERVATIONS DOSE: 0 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 1, 8 2 2 , 29, 36, 43 , 50,r 57, 64, 71, 78, 85,, 92, 99, 106, 113 1 2 0 , 127, 134, 141, 148, 155, 162, 169 , 176, 183, 190, 197, 204 2 1 1 , 218, 225, 232, 239, 246, 253, 260 , 267, 274, 281,. 288, 295 302, 309, 316, 323, 330, 337, 344, 351 , 358, 365, 372, 379, 386 393, 400, 407, 414, 421, 428, 435, 442 , 456, 463, 470, 477, 484 491, 498, 505, 512, 519, 526, 533,. 540 , 547 29, 36, 43, 50, 57 1 , 8 ,r 15, 2 2 , 64, 71, 78, 85, 92, 99, 106, 113, 1 2 0 , 127, 134 141, 148, 155, 162 , 169, 176, 183, 190, 197 , 204 , 211 , 218 , 225 232, 239, 246, 253 , 260, 267, 274, 281, 288 , 295 , 302 , 309 , 316 323, 330, 337, 344 , 351, 358, 365, 372, 379 , 386 , 393 , 400 , 407 414, 421, 428, 435 , 442, 449, 456, 463, 470 , 477 , 484 , 491 , 498 505, 512, 519, 526 , 533, 540, 547, 554, 561 , 568 , 575 , 582 1 , 8 ,r 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190, 197 204, 2 1 1 , 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281, 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372, 379 386, 393, 400, 407 , 414, 421, 428, 435 , 442, 449, 456, 463, 470 477, 484, 491, 498 , 505, 512, 519, 526 , 533, 540, 547, 554, 560 567 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 26 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: Ml DOSE: 0 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92550 D 104 APPEARANCE SWOLLEN PAW-HIND-RIGHT NORMAL NO REMARKABLE OBSERVATIONS SKIN & PELAGE SORE/SCAB VENTRAL-ABDOMINAL NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 54 708, 715, 722, 727 1 , 8 ,r 15, 2 2 , 29, 36 , 43,r 50, 113, 1 2 0 , 127,. 134, 141, 148, 204, 2 1 1 , 218, 225, 232, 239, 295, 302, 309,. 316, 323, 330, 386, 393, 400, 407, 414, 421, 477, 484, 491, 498, 505, 512, 568, 575, 582, 589, 596, 603, 659, 666, 673, 680, 687, 694, 57, 64, 71, 78, 85, 155, 162 , 169, 176, 246, 253 , 260, 267, 337, 344 , 351, 358, 428, 435 , 442, 449, 519, 526 , 533, 540, 610, 617 , 624, 631, 701 92, 99, 10 6 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 638, 645, 652 379 1 , 8 ,r 15, 2 2 , 29, 36 , 43,r 50, 113, 1 2 0 , 127,. 134, 141, 148, 204, 2 1 1 , 218, 225, 232, 239, 295, 302, 309,. 316, 323, 330, 400, 407, 414, 421, 428, 435, 491, 498, 505,. 512, 519, 526, 582, 589, 596, 603, 610, 617, 673, 680, 687,. 694, 701, 708, 57, 64, 71, 78, 85, 155, 162 , 169, 176, 246, 253 , 260, 267, 337, 344 , 351, 358, 442, 449 , 456, 463, 533, 540 , 547, 554, 624, 631 , 638, 645, 715, 722 , 729, 731 92, 99, 10 6 183, 190, 197 274, 281, 288 365, 386, 393 470, 477, 484 561, 568, 575 652, 659, 666 372C 568 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 27 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: Ml DOSE: 0 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92552 M 104 APPEARANCE HEAD TILT RIGHT THIN BEHAVIOR HYPOACTIVE <UNSPECIFIED> EXCRETION FEW FECES SKIN & PELAGE SORE/SCAB SHOULDER-RIGHT NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) SHOULDER-RIGHT WEEK 83 722, 726 694, 701, 708, 715, 722, 726 726 694, 701, 708 ,. 715, 726 575, 582, 589,. 596, 603, 610 1 , 8 ,r 15, 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 617, 624, 631, 638, 645, 652, 659 , 666, 673, 680, 687 575, 582, 589, 596, 603, 610 569 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 28 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: Ml DOSE: 0 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92553 M 63 APPEARANCE MALOCCLUSION SWOLLEN VENTRAL-ABDOMINAL THIN DISCHARGE CLEAR ORAL <UNSPECIFIED> NASAL RED IN COLOR RESPIRATION AUDIBLE IRREGULAR SKIN & PELAGE BROWN HAIR COAT PERINEAL AREA SCROTUM ROUGH HAIR COAT YELLOW HAIR COAT 295, 302, 309, 344, 365, 372 , 379, 400, 407, 414, 421, 428, 435 435 141, 232, 323, 435 148, 239, 330, 155, 246, 337, 162, 253, 344, 169, 260, 351, 176 , 183, 267 , 274, 358 , 365, 190, 281, 372, 197, 288, 379, 204, 295, 386, 2 1 1 , 218, 302, 309, 393, 400, 225 316 428 386 190, 267, 274 2 1 1 , 330, 365 197, 204, 211 407, 414, 421, 428, 435 330 29, 36, 43 , 50,r 57, 64, 71, 78, 85,r 92, 99, 106, 113, 1 2 0 , 127 134, 141, 148, 155, 162, 169 , 176, 183, 190, 197, 204, 2 1 1 , 218 225, 232, 239, 246, 253, 260 , 267, 274, 281, 288, 295, 302, 309 316, 323, 330, 337, 344, 351 , 358, 365, 372, 379, 386, 393, 400 407, 414, 421, 428, 435 204, 2 1 1 , 218, 225, 232, 239 , 246, 267, 274, 281, 288, 295, 302 309, 365, 372, 379, 386, 393 , 400 570 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 29 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: Ml (CONTINUED FROM PREVIOUS PAGE) C92553 M 63 NORMAL NO REMARKABLE OBSERVATIONS C92554 M 103 BEHAVIOR RECUMBENT STERNAL DISCHARGE GENITAL RED IN COLOR EXCRETION FEW FECES NO FECES EYES SQUINTED PERI-ORBITAL SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE VENTRAL-ABDOMINAL NORMAL NO REMARKABLE OBSERVATIONS DOSE: 0 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 1, 8 , 15, 22 719 232 708, 715 719 719 715, 719 719 1 , 8 ,r 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225 , 239, 246, 253, 260 , 267, 274, 281, 288 , 295 302, 309, 316, 323 , 330, 337, 344, 351 , 358, 365, 372, 379 , 386 393, 400, 407, 414 , 421, 428, 435, 442 , 449, 456, 463, 470 , 477 484, 491, 498, 505 , 512, 519, 526, 533 , 540, 547, 554, 561 , 568 575, 582, 589, 596 , 603, 610, 617, 624 , 631, 638, 645, 652 , 659 571 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 30 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: Ml (CONTINUED FROM PREVIOUS PAGE) C92554 M 103 NORMAL NO REMARKABLE OBSERVATIONS C92555 M 60 APPEARANCE HUNCHED EXCRETION NO FECES EYES RED DISCHARGE EYE-RIGHT EYES SKIN & PELAGE ROUGH HAIR COAT YELLOW HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) VENTRAL-ABDOMINAL WEEK 54 DOSE: 0 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 666, 673, 680, 687, 694, 701 409, 414-415 409, 414-415 409, 414-415 407 409, 414-415 407, 409, 414-415 1 22, 8,r 15, , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 120113, , 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 211204, , 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 379 , 386 393, 400 372 572 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 31 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: Ml DOSE: 0 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92556 M 63 APPEARANCE MALOCCLUSION THIN BEHAVIOR HYPOACTIVE <UNSPECIFIED> EXCRETION FEW FECES NORMAL NO REMARKABLE OBSERVATIONS C92557 M 89 APPEARANCE HUNCHED THIN EXCRETION FEW FECES SKIN & PELAGE RED HAIR COAT VENTRAL-ABDOMINAL ROUGH HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS 407, 410, 414, 421, 428, 435 410, 414, 421, 428, 435 428, 435 410, 414 421, 428, 435 1 , 8 , 15 113, 120 204, 211 295, 302 386, 393 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 400 617 589, 596, 603, 610, 617 617 617 603, 610, 617 1 , 8 , 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92 573 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 32 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: Ml (CONTINUED FROM PREVIOUS PAGE) C92557 M 89 NORMAL NO REMARKABLE OBSERVATIONS M 80 APPEARANCE HUNCHED THIN BEHAVIOR HYPOACTIVE <UNSPECIFIED> EXCRETION FEW FECES EYES SQUINTED PERI-ORBITAL NORMAL NO REMARKABLE OBSERVATIONS DOSE: 0 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 113, 204, 295, 386, 477, 568, 12 0 , 127, 2 1 1 , 218, 302, 309, 393, 400, 484, 491, 575, 582 134 , 141, 225 , 232, 316 , 323, 407 , 414, 498 , 505, 148, 239, 330, 421, 512, 155, 246, 337, 428, 519, 162 , 169, 253 , 260, 344 , 351, 435 , 442, 526 , 533, 176, 267, 358, 449, 540, 183, 274, 365, 456, 547, 190 , 197 281 , 288 372 , 379 463 , 470 554 , 561 557 554, 557 557 551, 554, 557 547, 551, 554, 557 1 , 8 ,r 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407 , 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498 , 505, 512, 519, 526 , 533, 540 574 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 33 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: Ml DOSE: 0 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92559 D 79 SKIN & PELAGE SORE/SCAB TAIL-PROXIMAL NORMAL NO REMARKABLE OBSERVATIONS C92560 M MASS(ES) TAIL-PROXIMAL WEEK 72 VENTRAL-THORACIC WEEK 79 80 BEHAVIOR HYPOACTIVE <UNSPECIFIED> RESPIRATION IRREGULAR SKIN & PELAGE YELLOW HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS 477, 498, 505,. 512, 519, 526, 533, 540, 547, 551 1 , 8 , 15, 2 2 , 29, 36, 43 , 50, 57, 64, 71 , 78, 85 , 92, 99, 106 113, 1 2 0 , 127,. 134, 141, 148, 155, 162, 169, 176 , 183, 190 , 197 204, 2 1 1 , 218,. 225, 232, 239, 246, 253, 260, 267 , 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344, 351, 358 , 365, 372 , 379 386, 393, 400,. 407, 414, 421, 428, 435, 442, 449 , 456, 463 , 470 484, 491 498C, 505C , 512C, 519C, 526C, 533C , 540C , 547C, 551C 54 7C, 551C 560 560 560 1 , 8 , 15, 22, 29, 36, 43,, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127, 134, 141, 148, 155, 162 , 169, 176, 183,. 190, 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281, 288 575 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 34 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: Ml (CONTINUED FROM PREVIOUS PAGE) C92560 M 80 NORMAL NO REMARKABLE OBSERVATIONS C92561 M MASS(ES) TAIL-PROXIMAL WEEK 79 63 BEHAVIOR RECUMBENT LATERAL EXCRETION FEW FECES RESPIRATION AUDIBLE IRREGULAR NORMAL NO REMARKABLE OBSERVATIONS DOSE: 0 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470 477, 484, 491, 498, 505, 512, 519, 526, 533, 540 547, 554, 560 436 436 436 1 , 8 , 15 113, 120 204, 211 295, 302 386, 393 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 400, 407 , 414, 421, 428, 435 576 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 35 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: Ml DOSE: 0 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92562 3 53 NORMAL NO REMARKABLE OBSERVATIONS SURGERY PERFORMED BY PATTIE MURPHY ANTIBIOTIC ADMINISTERED 0.5 ML M 84 APPEARANCE THIN EXCRETION FEW FECES NO FECES NORMAL NO REMARKABLE OBSERVATIONS 1 , 8 , 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365- 366 359 359- 360 582, 584 581- 582 584 1 , 8 , 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407 , 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498 , 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575 577 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 36 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: Ml DOSE: 0 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92564 M 70 APPEARANCE HEAD TILT RIGHT THIN BEHAVIOR HYPOACTIVE <UNSPECIFIED> EXCRETION FEW FECES NONFORMED FECES NORMAL NO REMARKABLE OBSERVATIONS C92565 D 91 APPEARANCE LIMITED USE PAWS-HIND DISCHARGE NASAL RED IN COLOR RESPIRATION LABORED 487 487 487 487 487 1 , 8 ,r 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407 , 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484 631, 637 627, 631, 637 627, 631, 637 578 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 37 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: Ml (CONTINUED FROM PREVIOUS PAGE) C92565 D 91 NORMAL NO REMARKABLE OBSERVATIONS C92566 M C92567 D 23 APPEARANCE LIMITED USE LIMBS-ALL MALOCCLUSION BEHAVIOR ATAXIC <UNSPECIFIED> NORMAL NO REMARKABLE OBSERVATIONS 91 NORMAL NO REMARKABLE OBSERVATIONS DOSE: 0 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 1, 8 15, 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582, 589, 596, 603, 610, 617 , 624 155 85, 92, 99, 106, 113, 120, 127, 134, 141, 148, 155 155 1 , 8 ,r 15, 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78 1 , 8 ,r 15, 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582, 589, 596, 603, 610, 617 , 624, 631, 633 579 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 38 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: Ml DOSE: 0 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92568 D 101 SKIN & PELAGE SORE/SCAB DORSAL NORMAL NO REMARKABLE OBSERVATIONS C92569 T WART LIKE LESION(S) DORSAL WEEK 95 105 APPEARANCE THIN NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) AXILLARY REGION-LEFT WEEK 93 694, 701 1 , 8 ,r 15, 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582, 589, 596, 603, 610, 617 , 624, 631, 638, 645 , 652 659, 666, 673, 680, 687, 694, 701 589, 596, 603 1, 8 , 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 2 2 , 29, 36 , 43,r 50, 127,. 134, 141, 148, 218, 225, 232, 239, 309,. 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582,. 610, 617, 624, 57, 64, 71, 78, 85, 155, 162 , 169, 176, 246, 253 , 260, 267, 337, 344 , 351, 358, 428, 435 , 442, 449, 519, 526 , 533, 540, 631, 638 92, 99, 106 183, 190 , 197 274, 281 , 288 365, 372 , 379 456, 463 , 470 547, 554 , 561 645C, 652C, 659C, 666C, 673C, 680C, 687C, 694C, 701C, 708C, 715C 580 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 39 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: Ml (CONTINUED FROM PREVIOUS PAGE) C92569 T 105 MASS(ES) AXILLARY REGION-LEFT WEEK 93 C92570 3 53 NORMAL NO REMARKABLE OBSERVATIONS SURGERY PERFORMED BY KATHY EWERS ANTIBIOTIC ADMINISTERED 0.5 ML DOSE: 0 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 722C, 729C, 731C 1 , 8 ,r 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190, 197 204, 2 1 1 , 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281, 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365-366 359 359-360 581 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C92503 C92522 M1 498 72 TO 505 73 MASS(ES) TAIL-PROXIMAL WEEK 72 512 74 MASS(ES) TAIL-PROXIMAL WEEK 72 519 75 TO 561 81 MASS(ES) TAIL-PROXIMAL WEEK 72 568 82 MASS(ES) TO 733 105 TAIL-PROXIMAL WEEK 72 729 105 MASS(ES) TO 733 105 TAIL-PROXIMAL WEEK 105 M1 435 63 TO 442 64 MASS(ES) VENTRAL-ABDOMINAL WEEK 63 603 87 TO 610 88 MASS(ES) VENTRAL-ABDOMINAL WEEK 87 617 89 TO 624 90 MASS(ES) VENTRAL-ABDOMINAL WEEK 87 10 X 10MM 15 X 10MM 20 X 15MM 15 X 10MM 15 X 10MM BOTTOM 10 X 10MM 10 X 10MM 15 X 15MM 582 Covance 6329-183 3M T-6295 PAGE: 40 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 44 MASS(ES) 46 VENTRAL-ABDOMINAL-LEFT WEEK 44 47 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 44 69 MASS(ES) 72 VENTRAL-ABDOMINAL WEEK 61 74 MASS(ES) 76 HEAD-MAXILLARY-RIGHT WEEK 71 77 MASS(ES) 80 HEAD-MAXILLARY-RIGHT WEEK 71 81 MASS(ES) 82 HEAD-MAXILLARY-RIGHT WEEK 71 83 MASS(ES) HEAD-MAXILLARY-RIGHT WEEK 71 84 MASS(ES) 85 HEAD-MAXILLARY-RIGHT WEEK 71 10 X 10MM 20 X 15MM 10 X 10MM 10 X 10MM 20 X 15MM 20 X 20MM 25 X 25MM 30 X 40MM 583 Covance 6329-183 3M T-6295 PAGE: 41 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 74 MASS(ES) 75 INGUINAL REGION-LEFT WEEK 69 76 MASS(ES) 78 INGUINAL REGION-LEFT WEEK 69 79 MASS(ES) INGUINAL REGION-LEFT WEEK 69 80 MASS(ES) INGUINAL REGION-LEFT WEEK 69 81 MASS(ES) 82 INGUINAL REGION-LEFT WEEK 69 83 MASS(ES) 84 INGUINAL REGION-LEFT WEEK 69 85 MASS(ES) 85 INGUINAL REGION-LEFT WEEK 69 93 MASS(ES) 96 DORSAL WEEK 93 10 X 10MM 15 X 10MM 20 X 15MM 20 X 20MM 30 X 25MM 30 X 30MM 35 X 40MM 15 X 15MM 584 Covance 6329-183 3M T-6295 PAGE: 42 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS CATEGORY KEYWORD WK QUALIFIER FREE-TEXT COMMENT 91 MASS(ES) 92 VENTRAL-CERVICAL-LEFT WEEK 91 93 MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 91 94 MASS(ES) 96 VENTRAL-CERVICAL-LEFT WEEK 91 68 MASS(ES) 97 DORSAL WEEK 68 103 MASS(ES) 105 DORSAL WEEK 103 93 MASS(ES) 105 TAIL-PROXIMAL WEEK 93 102 MASS(ES) LIMB-FRONT-LEFT WEEK 102 103 MASS(ES) 105 LIMB-FRONT-LEFT WEEK 102 30 X 30MM 40 X 35MM 45 X 50MM 10 X 10MM 10 X 10MM 15 X 10MM 20 X 20MM 30 X 30MM 585 Covance 6329-183 3M T-6295 PAGE: 43 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C92551 C92559 C92569 Ml 372 54 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 54 Ml 498 72 TO 505 73 MASS(ES) TAIL-PROXIMAL WEEK 72 512 74 TO 526 76 MASS(ES) TAIL-PROXIMAL WEEK 72 533 77 TO 551 79 MASS(ES) TAIL-PROXIMAL WEEK 72 547 79 TO 551 79 MASS(ES) VENTRAL-THORACIC WEEK 79 M1 645 93 MASS(ES) TO 731 105 AXILLARY REGION-LEFT WEEK 93 10 X 10MM 10 X 10MM 10 X 15MM 25 X 20MM 20 X 20MM 10 X 10MM Covance 6329-183 3M T-6295 PAGE: 44 586 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 45 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M2 DOSE: 0.5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92571 M 101 APPEARANCE HEAD TILT LEFT MALOCCLUSION BEHAVIOR ATAXIC <UNSPECIFIED> HYPOACTIVE <UNSPECIFIED> EXCRETION FEW FECES NORMAL NO REMARKABLE OBSERVATIONS C92572 D 101 APPEARANCE BENT TAIL THIN 694, 155, 246, 337, 428, 519, 610, ,6r9n4a , 701-702 162, 169, 253, 260, 344, 351, 435, 442, 526, 533, 617, 624, 701-7n 0n2o 176, 267, 358, 449, 540, 631, 183, 274, 365, 456, 547, 638, 190, 281, 372, 463, 554, 645, 197, 288, 379, 470, 561, 652, 204, 295, 386, 477, 568, 659, 2 1 1 , 218, 302, 309, 393, 400, 484, 491, 575, 582, 666, 673, 225, 232, 239 316, 323, 330 407, 414, 421 498, 505, 512 589, 596, 603 680- 681, 687 687 702 681, 687 694, 701-702 1 , 8 , 15 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120 127, 134 , 141, 148 12 0 , 127 2 1 1 , 218 302, 309 393, 400 484, 491 575, 582 701- 702 134, 225, 316, 407, 498, 589, 141 , 148, 232 , 239, 323 , 330, 414 , 421, 505 , 512, 596 , 603, 155, 246, 337, 428, 519, 610 162, 253, 344, 435, 526, 169 , 176, 260 , 267, 351 , 358, 442 , 449, 533 , 540, 183, 274, 365, 456, 547, 190, 281, 372, 463, 554, 197 , 204 288 , 295 379 , 386 470 , 477 561 , 568 587 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 46 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M2 (CONTINUED FROM PREVIOUS PAGE) C92572 D 101 EXCRETION FEW FECES NORMAL NO REMARKABLE OBSERVATIONS M 84 APPEARANCE MALOCCLUSION MISSING TEETH SWOLLEN PAW-HIND-LEFT THIN BEHAVIOR HYPOACTIVE <UNSPECIFIED> EXCRETION FEW FECES EYES RED DISCHARGE EYES NORMAL NO REMARKABLE OBSERVATIONS DOSE: 0.5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 701-702 1 , 8 ,r 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 617, 624, 631 , 638, 645, 652, 659 , 666, 673, 680, 687, 694 407, 414, 421, 449 , 456, 463, 470, 477 , 484, 491, 498, 505, 512 519, 526, 533,. 540 , 547, 554, 561, 568 , 575, 582, 584 449, 456, 463, 470 , 477, 484, 491, 498 , 505, 512, 519, 526, 533 540, 547, 554, 561 , 568, 575, 582, 584 463, 470, 477 582, 584 584 584 584 1 , 8 ,r 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190, 197 204, 2 1 1 , 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281, 288 588 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 47 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M2 (CONTINUED FROM PREVIOUS PAGE) C92573 M 84 NORMAL NO REMARKABLE OBSERVATIONS C92574 1 C92575 1 C92576 M 4 NORMAL NO REMARKABLE OBSERVATIONS 4 SKIN & PELAGE SORE/SCAB VENTRAL-CERVICAL NORMAL NO REMARKABLE OBSERVATIONS 97 APPEARANCE LIMITED USE LIMBS-HIND SKIN & PELAGE SORE/SCAB DORSAL NORMAL NO REMARKABLE OBSERVATIONS DOSE: 0.5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 428, 435, 442 1, 8 , 15, 22 15, 22 1, 8 673 512, 519 52 6 ,. 533, 540, 547, 554, 561 , 568, 575, 582, 589 , 596 603, 610 617,. 624, 631, 638, 645, 652 , 659, 666, 673 1 , 8 , 15 113, 120 204, 211 295, 302 386, 393 477, 484 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 491, 498 589 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 48 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER (CONTINUED FROM PREVIOUS PAGE) C92576 M 97 MASS(ES) DORSAL WEEK 73 GROUP: M2 C92577 T 105 SKIN & PELAGE SORE/SCAB DORSAL NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) AXILLARY REGION-LEFT WEEK 101 DORSAL WEEK 69 HEAD-MAXILLARY-LEFT WEEK 67 WEEK 105 DOSE: 0.5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 505C, 512C , 519C, 526C, 533C, 54 0C, 547C , 554C, 561C, 568C, 575C 582C, 589C , 596C, 603C, 610C, 617C, 624C , 631C, 638C, 645C, 652C 659C, 666C , 673C 603, 610, 617, 624, 631,, 638, 645, 652, 659, 666, 673, 680, 687 694, 701, 708, 715, 722,, 729, 733 1 , 8 ,r 15, 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456 701C, 708C, 715C, 722C, 729C, 733C 477C, 484C, 491C, 498C, 505C, 512C, 519C, 526C, 533C, 54 0C, 547C 554C, 561C, 568C, 575C, 582C, 58 9C, 596C, 603C, 610C, 617C, 624C 631C, 638C, 645C, 652C, 659C, 666C, 673C, 680C, 687C, 694C, 701C 708C, 715C, 722C, 729C, 733C 463, 470, 477, 484 729, 733 590 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 49 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M2 DOSE: 0.5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92578 M 86 APPEARANCE THIN BEHAVIOR ATAXIC <UNSPECIFIED> EXCRETION FEW FECES EYES RED DISCHARGE EYE-LEFT SKIN & PELAGE PALE ENTIRE BODY NORMAL NO REMARKABLE OBSERVATIONS 589-590, 596, 598 598 590, 596, 598 598 598 1 , 8 ,r 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407 , 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498 , 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582 591 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 50 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M2 DOSE: 0.5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92579 D 86 APPEARANCE THIN BEHAVIOR HYPOACTIVE <UNSPECIFIED> COLD TO TOUCH ENTIRE BODY EXCRETION FEW FECES RESPIRATION LABORED SKIN & PELAGE PALE ENTIRE BODY NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) VENTRAL-ABDOMINAL WEEK 86 589, 596, 602 602 602 602 602 582, 589, 596, 602 1, 8 , 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575 2 2 , 29, 36, 43,r 50, 127, 134 , 141, 148, 218, 225 , 232, 239, 309, 316 , 323, 330, 400, 407 , 414, 421, 491, 498 , 505, 512, 57, 64, 71, 78, 85, 155, 162 , 169, 176, 246, 253 , 260, 267, 337, 344 , 351, 358, 428, 435 , 442, 449, 519, 526 , 533, 540, 92, 99, 106 183, 190 , 197 274, 281 , 288 365, 372 , 379 456, 463 , 470 547, 554 , 561 596, 602 592 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 51 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M2 DOSE: 0.5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92580 M 79 BEHAVIOR HYPOACTIVE <UNSPECIFIED> EXCRETION FEW FECES NONFORMED FECES EYES RED DISCHARGE EYE-LEFT SKIN & PELAGE SORE/SCAB VENTRAL-ABDOMINAL-RIGHT NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 61 549 540, 547 549 549 549 449, 456 463 1 , 8 , 15 113, 120 204, 211 295, 302 386, 393 526, 533 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 400, 407 , 414, 470, 477, 484 , 491, 498, 505, 512 , 519 421, 428 435C, 442C 593 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 52 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M2 DOSE: 0.5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92581 M 105 APPEARANCE HUNCHED OBES THIN EXCRETION FEW FECES NO FECES NONFORMED FECES SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE SORE/SCAB SHOULDER-RIGHT NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) DORSAL WEEK 85 LIMB-HIND-RIGHT WEEK 93 SHOULDER-RIGHT WEEK 50 729 596 681, 687, 694, 701, 708, 715, 722 722 681, 687, 729 596 722, 729 421, 428 435, 442, 449, 456, 463, 470, 477, 484, 491, 498, 505 512, 519 526, 533, 540, 547, 554, 561, 568, 575, 582, 589, 596 603, 610 617, 624 1 , 8 , 15 113, 120 204, 211 295, 302 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 127, 134 , 141, 148, 155, 162 , 16 9, 176, 183,. 190, 197 218, 225 , 232, 239, 246, 253 , 26 0, 267, 274, 281, 288 309, 316 , 323, 330, 337, 631 , 63 8 58 9C, 596C, 603C, 610C, 617C 645C, 652C, 659C, 666C, 673C, 68 0-681C, 687C, 694C, 701C, 708C 715C, 722C, 72 9C 344C, 351C, 358C, 365C, 372C, 37 9C, 386C, 393C, 400C, 407C, 414C 594 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 53 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M2 (CONTINUED FROM PREVIOUS PAGE) C92581 M 105 MASS(ES) SHOULDER-RIGHT WEEK 50 C92582 M WART LIKE LESION(S) DORSAL WEEK 95 101 APPEARANCE LIMITED USE LIMB-FRONT-LEFT BEHAVIOR HYPOACTIVE <UNSPECIFIED> EXCRETION FEW FECES SKIN & PELAGE SORE/SCAB SHOULDER-LEFT NORMAL NO REMARKABLE OBSERVATIONS DOSE: 0.5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 421C, 42 8C, 435C, 442C, 449C, 456C, 463C, 470C, 477C, 484C, 491C 498C, 505C, 512C, 519C, 526C, 533C, 540C, 547C 659, 666, 673, 680-681, 687, 694, 701, 708, 715, 722, 729 701-702 694, 701-702 694, 701-702 1 , 8 ,r 15, 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582, 589, 596, 603, 610, 617 , 624, 631, 638, 645 , 652 595 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 54 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M2 (CONTINUED FROM PREVIOUS PAGE) C92582 M 101 NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) SHOULDER-LEFT WEEK 98 C92583 T 105 SKIN & PELAGE SORE/SCAB DORSAL NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) DORSAL WEEK 74 DOSE: 0.5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 659, 666, 673 680C, 687C, 694C, 701-702C 694, 701, 708,. 715, 722, 729, 731 1 , 8 ,. 15, 2 2 , 29, 36, 43 , 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127,. 134, 141, 148, 155, 162, 169, 176, 183, 190 , 197 204, 2 1 1 , 218,. 225, 232, 239, 246, 253, 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344, 351, 358, 365, 372 , 379 386, 393, 400,. 407, 414, 421, 428, 435, 442, 449, 456, 463 , 470 477, 484, 491, 498, 505 512C, 519C , 526C, 533C, 54 0C, 547C , 554C, 561C, 568C, 575C , 582C 58 9C, 596C , 603C, 610C, 617C, 624C , 631C, 638C, 645C, 652C , 659C 666C, 673C , 680C, 687C, 694C, 701C , 708C, 715C, 722C, 72 9C , 731C 596 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 55 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M2 DOSE: 0.5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92584 M 60 APPEARANCE THIN BEHAVIOR HYPOACTIVE <UNSPECIFIED> EXCRETION FEW FECES NORMAL NO REMARKABLE OBSERVATIONS M 39 APPEARANCE MALOCCLUSION MISSING TEETH EXCRETION FEW FECES RESPIRATION AUDIBLE LABORED 418 418 414, 418 1 , 8 , 15 , 2 2 , 29, 36 , 43 , 50, 57, 64, 71, 78 , 85 , 92, 99, 106 113, 120 , 127, 134, 141, 148, 155, 162, 169, 176 , 183, 190, 197 204, 211 , 218, 225, 232, 239, 246, 253, 260, 267 , 274, 281, 288 295, 302 , 309, 316, 323, 330, 337, 344, 351, 358 , 365, 372, 379 386, 393 , 400, 407 2 2 , 29, 36, 43 , 50, 57, 64, 71 , 78 , 85, 92, 99, 106, 113, 120 127, 134 , 141, 148, 155, 162, 169, 176, 183, 190 , 197, 204, 211 218, 225 , 232, 239, 246, 253, 260, 267- 268 78, 85, 92, 99 , 106, 113 , 1 2 0 , 127 , 134 , 141 , 14 8 , 155 , 162 , 169 176, 183 , 190, 197, 204, 2 1 1 , 218, 225, 232, 239 , 246, 253, 260 267- 268 253, 260 , 267- 268 239 239, 246 , 253, 260, 267- 268 597 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 56 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M2 (CONTINUED FROM PREVIOUS PAGE) C92585 M 39 NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) AXILLARY REGION-LEFT WEEK 39 AXILLARY REGION-RIGHT WEEK 37 VENTRAL-CERVICAL WEEK 37 C92586 M 73 APPEARANCE THIN EXCRETION FEW FECES SKIN & PELAGE YELLOW HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS DOSE: 0.5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 1, 8, 15 267-268C 253, 260C, 267-268C 253C, 260C, 267-268C 503-506 498, 503-506 504-506 1 22, 8,r 15, , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 120113, , 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 211204, , 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407 , 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491 598 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 57 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M2 DOSE: 0.5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92587 M 88 APPEARANCE THIN EXCRETION FEW FECES SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE NORMAL NO REMARKABLE OBSERVATIONS C92588 M APPEARANCE MALOCCLUSION MISSING TEETH THIN 589-590, 596 603, 610 590, 596, 603, 610 610 1 , 8 , 15, 2 2 , 29, 36 , 43 , 50, 57, 64, 71, 78 , 85, 92, 99, 106 113, 1 2 0 , 127, 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582 29, 36, 43 , 50 , 57, 64, 71, 78 , 85 , 92 , 99, 106, 113, 1 2 0 , 127 134, 141, 148, 155, 162, 169, 176, 183 , 190, 197, 204, 211 , 218 225, 232, 239, 246, 253, 260, 267, 274 , 281, 288, 295, 302 , 309 316, 323, 330, 337, 344, 351, 358, 365 , 372, 379, 386, 393 , 400 407, 414, 421, 428, 435, 442, 449, 456 , 463, 470, 477, 484 , 491 498, 505, 512, 519, 526, 533, 540, 547 , 554, 561, 568, 575 , 582 589, 596, 603, 610, 617, 624, 631, 638 , 645, 652, 659, 666 , 673 680, 687, 694, 701, 708, 715 589, 596, 603, 610, 617, 624, 631, 638 , 645, 652, 659, 666 , 673 680, 687, 694, 701, 708, 715 708, 715 599 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 58 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M2 (CONTINUED FROM PREVIOUS PAGE) C92588 M 103 BEHAVIOR HYPOACTIVE <UNSPECIFIED> EXCRETION FEW FECES NORMAL NO REMARKABLE OBSERVATIONS C92589 M 100 APPEARANCE THIN BEHAVIOR ATAXIC <UNSPECIFIED> HYPOACTIVE <UNSPECIFIED> EXCRETION FEW FECES EYES RED DISCHARGE EYES NORMAL NO REMARKABLE OBSERVATIONS DOSE: 0.5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 708, 715 708, 715 1, 8 , 15, 22 687, 694 694 694 694 694 1 , 8 ,r 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407 , 414, 421, 428, 435 , 442, 449, 456, 463 , 470 600 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 59 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M2 (CONTINUED FROM PREVIOUS PAGE) C92589 M 100 NORMAL NO REMARKABLE OBSERVATIONS C92590 T 105 NORMAL NO REMARKABLE OBSERVATIONS C92591 D 96 SKIN & PELAGE SORE/SCAB VENTRAL-ABDOMINAL-LEFT VENTRAL-ABDOMINAL-RIGHT NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) INGUINAL REGION-LEFT WEEK 87 DOSE: 0.5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 477, 484, 491, 498, 505, 512, 519, 526, 533, 540, 547, 554, 561 568, 575, 582, 589, 596, 603, 610, 617, 624, 631, 638, 645, 652 659, 666, 673, 680 1 , 8 ,r 15, 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582, 589, 596, 603, 610, 617 , 624, 631, 638, 645 , 652 659, 666, 673, 680, 687, 694, 701, 708 , 715, 722, 729, 732 617, 624, 631, 638 , 645 519, 526 1 , 8 ,r 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407 , 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 533 , 540 603, 610, 617, 624, 631, 638 645, 652, 659, 666, 601 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 60 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M2 (CONTINUED FROM PREVIOUS PAGE) C92591 D 96 MASS(ES) VENTRAL-ABDOMINAL WEEK 79 VENTRAL-ABDOMINAL-LEFT WEEK 95 VENTRAL-ABDOMINAL-RIGHT WEEK 72 C92592 M 88 APPEARANCE LIMITED USE LIMBS-HIND NORMAL NO REMARKABLE OBSERVATIONS C92593 2 C92594 D 14 NORMAL NO REMARKABLE OBSERVATIONS 96 NORMAL NO REMARKABLE OBSERVATIONS DOSE: 0.5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 547, 554, 561,. 568, 575, 582C,, 589C, 596C, 603C, 610C 659C, 666C , 670C 498C, 505C , 512C 610 1 , 8 , 15, 2 2 , 29, 36, 43,, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127,. 134, 141, 148, 155, 162 , 169, 176, 183 , 190 , 197 204, 2 1 1 , 218,. 225, 232, 239, 246, 253 , 260, 267, 274 , 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365 , 372 , 379 386, 393, 400,. 407, 414, 421, 428, 435 , 442, 449, 456 , 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547 , 554 , 561 568, 575, 582,. 589, 596, 603, 610 1 , 8 , 15, 2 2 , 29, 36, 43,, 50, 57, 64, 71, 78, 85, 92- 93 1 , 8 , 15, 2 2 , 29, 36, 43,, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127,. 134, 141, 148, 155, 162 , 169, 176, 183 , 190 , 197 204, 2 1 1 , 218,. 225, 232, 239, 246, 253 , 260, 267, 274 , 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365 , 372 , 379 386, 393, 400,. 407, 414, 421, 428, 435 , 442, 449, 456 , 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547 , 554 , 561 568, 575, 582,. 589, 596, 603, 610, 617 , 624, 631, 638 , 645 , 652 602 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 61 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M2 (CONTINUED FROM PREVIOUS PAGE) C92594 D 96 NORMAL NO REMARKABLE OBSERVATIONS C92595 M 84 APPEARANCE THIN EXCRETION FEW FECES EYES RED DISCHARGE EYES SKIN & PELAGE PALE ENTIRE BODY SORE/SCAB DORSAL-THORACIC DORSAL-THORACIC-RIGHT NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) DORSAL WEEK 73 DOSE: 0.5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 659, 666-667 582-583 581-583 581-583 582-583 491, 498, 505, 512 , 519, 526, 533, 540 , 547, 554, 561, 568 , 575 581- 583 393, 400, 407, 414 , 421, 428, 435, 442 , 449, 456, 463 1 , 8 , 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 470, 477, 484 505C, 512C, 519C, 526C, 533C, 540C, 547C, 554C, 561C, 568C, 575C 581-583C 603 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 62 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M2 DOSE: 0.5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92596 M 71 APPEARANCE THIN EXCRETION FEW FECES SKIN & PELAGE BROWN HAIR COAT SCROTUM SORE/SCAB VENTRAL-CERVICAL NORMAL NO REMARKABLE OBSERVATIONS T 105 APPEARANCE SWOLLEN PAW-HIND-LEFT SKIN & PELAGE SORE/SCAB PAW-HIND-LEFT NORMAL NO REMARKABLE OBSERVATIONS 477, 484, 491 477, 484, 491 491 15 1 , 8 ,r 2 2 , 29, 36, 43 , 50,r 57, 64, 71, 78, 85,r 92, 99, 106, 113 1 2 0 , 127, 134, 141, 148, 155, 162, 169 , 176, 183, 190, 197 , 204 2 1 1 , 218, 225,. 232, 239, 246, 253, 260 , 267, 274, 281, 288 , 295 302, 309, 316,. 323, 330, 337, 344, 351 , 358, 365, 372, 379 , 386 393, 400, 407,. 414, 421, 428, 435, 442 , 449, 456, 463, 470 645, 652, 659,. 666, 673, 680, 687,. 694 , 701, 708, 715, 722 , 729 731 617, 624, 631,. 638, 645, 652, 659, 666 , 673, 680, 687, 694 , 701 708, 715, 722,. 729, 731 1 , 8 ,r 15, 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 604 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 63 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M2 (CONTINUED FROM PREVIOUS PAGE) C92597 T 105 NORMAL NO REMARKABLE OBSERVATIONS C92598 T 105 DISCHARGE GENITAL RED IN COLOR NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) AXILLARY REGION-RIGHT WEEK 103 DOSE: 0.5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 204 2 1 1 , 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470 477 484, 491, 498, 505, 512, 519, 526, 533, 540, 547, 554, 561 568 575, 582, 589, 596, 603, 610 512 1 , 8 ,r 15, 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491 498, 505, 519, 526, 533 , 540, 547, 554, 561 , 568 575, 582, 589 596, 603, 610, 617, 624 , 631, 638, 645, 652 , 659 666, 673, 680 687, 694, 701, 708, 722 , 729, 731 715C 605 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 64 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M2 DOSE: 0.5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92599 D 55 NORMAL NO REMARKABLE OBSERVATIONS C92600 2 C92601 T 14 NORMAL NO REMARKABLE OBSERVATIONS 105 EXCRETION NONFORMED FECES SKIN & PELAGE SORE/SCAB DORSAL NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) DORSAL WEEK 70 1 , 8 ,r 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 385 1, 8 , 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92-93 400 568, 575, 582, 589, 596, 603 , 610, 617, 624, 631, 638, 645 , 652 659, 666, 673 1 , 8 ,. 15, 2 2 , 29, 36, 43 , 50 , 57, 64, 71, 78,, 85, 92, 99, 106 113, 1 2 0 , 127, 134, 141, 148 , 155, 162, 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225, 232, 239 , 246, 253, 260, 267, 274, 281 , 288 295, 302, 309, 316, 323, 330 , 337, 344, 351, 358, 365, 372 , 379 386, 393, 407, 414, 421, 428 , 435, 442, 449, 456, 463, 470 , 477 484, 491, 498C , 505C, 512C, 519C, 526C, 533C,, 540C, 547C, 554C 561C, 568C , 575C, 582C, 589C , 596C , 603C, 610C, 617C, 624C , 631C 638C, 645C , 652C, 659C, 666C , 673C , 680C, 687C, 694C, 701C , 708C 715C 606 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 65 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M2 (CONTINUED FROM PREVIOUS PAGE) C92601 T 105 WART LIKE LESION(S) DORSAL WEEK 104 C92602 T 105 SKIN & PELAGE BLACK SKIN VENTRAL-ABDOMINAL-LEFT NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 73 M 19 APPEARANCE PARALYSIS LIMBS-HIND BEHAVIOR COLD TO TOUCH LIMBS-HIND NORMAL NO REMARKABLE OBSERVATIONS DOSE: 0.5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 722, 729, 733 512 1 , 8 , 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407 , 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498 , 533, 540, 547, 554 , 561, 568, 575, 582 , 589 596, 603, 610, 617 , 624, 631, 638, 645 , 652, 659, 666, 673 , 680 687, 694, 701, 708 , 715, 722, 729, 733 505C, 512C , 519C, 526C 127 127 1 , 8 , 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92 607 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 66 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M2 (CONTINUED FROM PREVIOUS PAGE) C92603 M 19 NORMAL NO REMARKABLE OBSERVATIONS C92604 M 91 APPEARANCE THIN EXCRETION FEW FECES SKIN & PELAGE BROWN HAIR COAT VENTRAL-ABDOMINAL ROUGH HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS C92605 D 104 BEHAVIOR COLD TO TOUCH ENTIRE BODY RESPIRATION AUDIBLE DOSE: 0.5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 113, 120 589, 596 603, 610, 617, 624, 631 624, 631 617, 624, 631 610, 617, 624, 631 1 , 8 ,r 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407 , 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498 , 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582 722-723 722-723 608 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 67 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M2 (CONTINUED FROM PREVIOUS PAGE) C92605 D 104 SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE SORE/SCAB DORSAL VENTRAL-ABDOMINAL-RIGHT NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) DORSAL WEEK 79 VENTRAL-ABDOMINAL-LEFT WEEK 83 WEEK 87 VENTRAL-ABDOMINAL-RIGHT WEEK 75 DOSE: 0.5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 722- 723 631, 638, 722- 723 610, 617, 701, 708, 645, 624, 715, 652 , 659, 631 , 638, 722 -723 666, 673, 645, 652, 680, 659, 687, 694 , 701, 666, 673 , 680, 708 , 715 687 , 694 1 , 8 , 15, 2 2 , 29, 36, 43 , 50, 57, 64, 71 , 78, 85 , 92, 99, 106 113, 1 2 0 , 127, 134 , 141, 148, 155, 162, 169, 176 , 183, 190 , 197 204, 2 1 1 , 218, 225 , 232, 239, 246, 253, 260, 267 , 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344, 351, 358 , 365, 372 , 379 386, 393, 400, 407 , 414, 421, 428, 435, 442, 449 , 456, 463 , 470 477, 484, 491, 498 , 505, 512 547, 554, 561, 568 , 575, 582, 589, 596, 603, 610 , 617, 624 , 631 638, 645, 652, 659 , 666, 673, 680, 687, 694, 701 , 708, 715 722- 723 575C , 582C 603C , 610C , 617C, 624C, 631C, 638C , 645C , 652C, 659C, 666C , 673C 680C , 687C', 694C, 701C, 708C, 715C , 722- 723C 519, 526, 533,. 540 , 547, 554, 561, 568, 575, 582 , 589, 596 609 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 68 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M2 DOSE: 0.5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92606 M 78 APPEARANCE LIMITED USE LIMBS-HIND PAWS-FRONT SWOLLEN MOUTH BEHAVIOR RECUMBENT STERNAL SKIN & PELAGE SORE/SCAB HEAD-ENTIRE NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) SHOULDER-RIGHT WEEK 66 WART LIKE LESION(S) HEAD-MAXILLARY-LEFT WEEK 31 NOSE WEEK 75 542 540 141 542 491, 498, 505 1 , 8 ,r 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127, 134 , 148, 155, 162, 169 , 176, 183, 190, 197, 204 267, 274, 281, 288 , 295, 302, 309, 316 , 323, 330, 337, 344, 351 358, 365, 372, 379 , 386, 393, 400, 407 , 414, 421, 428, 435, 442 449 456, 463, 470, 477 , 484, 491, 498, 505 , 512C, 519C , 526C, 533C 540C, 542C 2 1 1 , 218, 225, 232, 239, 246, 253, 260 519, 526, 533, 540, 542 610 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 69 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M2 DOSE: 0.5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92607 M 91 APPEARANCE THIN SKIN & PELAGE ROUGH HAIR COAT SORE/SCAB VENTRAL-ABDOMINAL-RIGHT NORMAL NO REMARKABLE OBSERVATIONS C92608 M MASS(ES) INGUINAL REGION-RIGHT WEEK 28 VENTRAL-ABDOMINAL-LEFT WEEK 30 79 APPEARANCE HUNCHED MALOCCLUSION THIN 589, 596, 603, 610, 617, 624, 631 617, 624, 631 204, 211, 218 1 22, 8,r 15, , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 120113, , 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 225 , 232 239, 246, 253, 260 , 267, 274, 281, 288 , 295, 302, 309, 316 , 323 330, 337, 344, 351 , 358, 365, 372, 379 , 386, 393, 400, 407 , 414 421, 428, 435, 442 , 449, 456, 463, 470 , 477, 484, 491, 498 , 505 512, 519, 526, 533 , 540, 547, 554, 561 , 568, 575, 582 190C, 197C 204, 211 540, 547-548 99, 101, 106, 113, 120, 127, 134, 141, 148, 155, 162, 169, 176 211183,. 190, 197, 204, ,. 218, 225,. 232, 239,. 246,. 253, 260, 267 274,. 281, 288, 295, 302, 309,. 316,. 323, 330, 337,. 344, 351, 358 365,. 372, 379,. 386, 393, 400, 407,. 414, 421,. 428, 435,. 442, 449 456,. 463, 470, 477,. 484, 491, 498, 505,. 512, 519,. 526,. 533,. 540 547-548 533, 540, 547-548 611 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 70 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M2 (CONTINUED FROM PREVIOUS PAGE) C92608 M 79 BEHAVIOR HYPOACTIVE <UNSPECIFIED> EXCRETION FEW FECES RESPIRATION AUDIBLE LABORED SKIN & PELAGE ROUGH HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS C92609 M 104 APPEARANCE LIMITED USE LIMBS-HIND BEHAVIOR HYPOACTIVE <UNSPECIFIED> EYES CLEAR DISCHARGE EYE-LEFT EYES RESPIRATION LABORED DOSE: 0.5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 548 512, 540, 547-548 1 0 1 , 106 99 491, 498, 505, 512, 519, 526, 533, 540, 547-548 1, 8 , 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92 724 724 589, 596, 603, 610, 724 659, 666, 673, 680, 687, 694, 701, 708 724 612 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 71 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M2 (CONTINUED FROM PREVIOUS PAGE) C92609 M 104 NORMAL NO REMARKABLE OBSERVATIONS C92610 1 C92611 M MASS(ES) DORSAL WEEK 102 WART LIKE LESION(S) HEAD-MAXILLARY-LEFT WEEK 98 4 NORMAL NO REMARKABLE OBSERVATIONS 88 NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 62 WEEK 72 DOSE: 0.5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 1 , 8 ,r 15, 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582, 617, 624, 631, 638, 645 , 652 708C, 715C, 722C, 724C 680C, 687C, 694C, 701C, 708C, 715C, 722C, 724C 1 , 8 , 15, 22 1 , 8 , 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407 , 414, 421, 449, 456 , 463, 470, 477, 484 , 491 428, 435, 442 498C, 505C, 512C, 519C, 526C, 533C, 540C, 547C, 554C, 561C, 568C 575C, 582C 613 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 72 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M2 (CONTINUED FROM PREVIOUS PAGE) C92611 M 88 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 85 C92612 M 79 APPEARANCE THIN BEHAVIOR HYPOACTIVE SEVERE RECUMBENT LATERAL COLD TO TOUCH ENTIRE BODY RESPIRATION IRREGULAR SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE ROUGH HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS DOSE: 0.5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 58 9C, 596C, 603C, 610C 533, 540, 547 547 547 547 547 169 533, 540, 547 1 , 8 ,r 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127, 134 , 141, 148, 155, 162 , 176, 183, 190, 197 , 204 2 1 1 , 218, 225, 232 , 239, 246, 253, 260 , 267, 274, 281, 288 , 295 302, 309, 316, 323 , 330, 337, 344, 351 , 358, 365, 372, 379 , 386 393, 400, 407, 414 , 421, 428, 435, 442 , 449, 456, 463, 470 , 477 484, 491, 498, 505 , 512, 519, 526 614 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 73 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M2 DOSE: 0.5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92613 1 C92614 D 4 NORMAL NO REMARKABLE OBSERVATIONS 92 SKIN & PELAGE SORE/SCAB DORSAL DORSAL-CERVICAL VENTRAL-ABDOMINAL-LEFT NORMAL NO REMARKABLE OBSERVATIONS C92615 M MASS(ES) DORSAL WEEK 89 VENTRAL-ABDOMINAL WEEK 45 90 BEHAVIOR RECUMBENT LATERAL EXCRETION FEW FECES 1, 8 , 15, 22 631, 638, 640 617, 624 323, 330 1 , 8 , 15 113, 120 204, 211 295, 302 414, 421 505, 512 596, 603 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 337, 344 , 351, 358, 365, 372 , 379, 386, 393, 400 , 407 428, 435 , 442, 449, 456, 463 , 470, 477, 484, 491 , 498 519, 526 , 533, 540, 547, 554 , 561, 568, 575, 582 , 589 610 617C, 624C, 631C, 638C, 640C 309, 316 624 624 615 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 74 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M2 (CONTINUED FROM PREVIOUS PAGE) C92615 M 90 SKIN & PELAGE ROUGH HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS C92616 2 C92617 M 14 EYES CLEAR DISCHARGE EYE-LEFT NORMAL NO REMARKABLE OBSERVATIONS 81 APPEARANCE THIN BEHAVIOR HYPOACTIVE <UNSPECIFIED> RECUMBENT LATERAL SENSITIVE TO TOUCH EXCRETION NO FECES DOSE: 0.5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 617, 624 1 , 8 ,r 15, 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582, 589, 596, 603, 610 50 1, 8 , 15, 22, 29, 36, 43, 57, 64 71, 78, 85 92-93 561, 565 565 565 561 565 616 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 75 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M2 (CONTINUED FROM PREVIOUS PAGE) C92617 M 81 EYES PALE EYES RED DISCHARGE EYES NORMAL NO REMARKABLE OBSERVATIONS C92618 1 C92619 T 4 NORMAL NO REMARKABLE OBSERVATIONS 105 NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 64 DOSE: 0.5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 533, 540, 547, 554 , 561, 565 565 1 , 8 ,r 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407 , 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498 , 505, 512, 519, 526 1, 8 , 15, 22 1, 8 15, 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 449, 456, 463, 470 , 477 484, 491, 498, 505, 512, 519, 526, 533 , 540, 547, 554, 561 , 568 575, 582, 589,. 596, 603, 610, 617, 624 , 631, 638, 645, 652 , 659 666, 673, 680, 687, 694, 701, 708, 715 , 722, 729, 732 442C 617 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 76 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M2 DOSE: 0.5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92620 D 96 APPEARANCE MALOCCLUSION MISSING TEETH EXCRETION FEW FECES EYES RED DISCHARGE EYE-RIGHT SCAB EYE-RIGHT SKIN & PELAGE PALE ENTIRE BODY SORE/SCAB VENTRAL-ABDOMINAL-LEFT NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 71 64, 71, 78, 85,r 92, 99, 106, 113, 1 2 0 , 127, 134, 141, 148, 155 162, 169 , 176, 183, 190, 197, 204, 2 1 1 , 218, 225, 232, 239, 246 253, 260 , 267, 274, 281, 288, 295, 302, 309, 316, 323, 330, 337 344, 351 , 358, 365, 372, 379, 386, 393, 400, 407, 414, 421, 428 435, 442 , 449, 456, 463, 470, 477, 484, 491, 498, 505, 512, 519 526, 533 , 540, 547, 554, 561, 568, 575, 582, 589, 596, 603, 610 617, 624 , 631, 638, 645, 652, 659, 666-667 589, 596 , 603, 610, 617, 624, 631, 638, 645, 652, 659, 666-667 666-667 561, 568 316 561, 568 505, 512, 519 1, 8 , 15, 22, 29, 36, 43, 50, 57 491C, 498C 618 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 77 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M2 DOSE: 0.5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92621 2 C92622 D 14 NORMAL NO REMARKABLE OBSERVATIONS 83 NORMAL NO REMARKABLE OBSERVATIONS C92623 T MASS(ES) DORSAL WEEK 72 105 SKIN & PELAGE SORE/SCAB VENTRAL-ABDOMINAL NORMAL NO REMARKABLE OBSERVATIONS 1 , 8 , 15, 2 2 , 29, 36, 43,, 50, 57, 64, 71, 78, 85, 92- 93 1 , 8 , 15, 2 2 , 29, 36, 43,, 50, 57, 64, 71, 78, 85, 92, 99, 10 6 113, 1 2 0 , 127,. 134, 141, 148, 155, 162 , 169, 176, 183 , 190, 197 204, 2 1 1 , 218,. 225, 232, 239, 246, 253 , 260, 267, 274 , 281, 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365 , 372, 379 386, 393, 400,. 407, 414, 421, 428, 435 , 442, 449, 456 , 463, 470 477, 484, 491,. 533, 540, 547, 554, 561 , 568, 575, 578 498, 505, 512, 519, 526 659, 666 1 , 8 , 15, 2 2 , 29, 36, 43,, 50, 57, 64, 71, 78, 85, 92, 99, 10 6 113, 1 2 0 , 127,. 134, 141, 148, 155, 162 , 169, 176, 183 , 190, 197 204, 2 1 1 , 218,. 225, 232, 239, 246, 253 , 260, 267, 274 , 281, 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365 , 372, 379 386, 393, 400,. 407, 414, 421, 428, 435 , 442, 449, 456 , 463, 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547 , 554, 561 568, 575, 582,. 589, 596, 603, 610, 617 , 624, 631, 638 , 645, 652 673, 680, 687,. 694, 701, 708, 715, 722 , 729, 733 619 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 78 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M2 DOSE: 0.5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92624 D 61 NORMAL NO REMARKABLE OBSERVATIONS C92625 M 84 APPEARANCE HUNCHED THIN TREMORS HEAD BEHAVIOR HYPOACTIVE <UNSPECIFIED> COLD TO TOUCH ENTIRE BODY EXCRETION FEW FECES RESPIRATION IRREGULAR SKIN & PELAGE PALE ENTIRE BODY SORE/SCAB DORSAL 1 , 8 ,r 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407 , 414, 421, 425 588 581-582, 588 588 588 588 581-582, 588 588 588 561, 568 620 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 79 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M2 (CONTINUED FROM PREVIOUS PAGE) C92625 M 84 NORMAL NO REMARKABLE OBSERVATIONS T 105 APPEARANCE MALOCCLUSION MISSING TEETH SWOLLEN PAW-HIND-LEFT EYES CLEAR DISCHARGE EYE-RIGHT RED DISCHARGE EYE-RIGHT DOSE: 0.5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 1 , 8 , 15, 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127, 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 575 106, 197, 288, 379, 470, 561, 652, 113, 204, 295, 386, 477, 568, 659, 12 0 , 127, 2 1 1 , 218, 302, 309, 393, 400, 484, 491, 575, 582, 666, 673, 134, 225, 316, 407, 498, 589, 680, 141, 232, 323, 414, 505, 596, 687, 148, 239, 330, 421, 512, 603, 694, 155 , 162, 246 , 253, 337 , 344, 428 , 435, 519 , 526, 610 , 617, 701 , 708, 169, 260, 351, 442, 533, 624, 715, 176, 267, 358, 449, 540, 631, 722, 183 , 190 274 , 281 365 , 372 456 , 463 547 , 554 638 , 645 729 , 732 351, 442, 533, 624, 715, 358, 449, 540, 631, 722, 365, 456, 547, 638, 729, 372, 463, 554, 645, 732 379, 470, 561, 652, 386, 477, 568, 659, 393, 400 , 407, 484, 491 , 498, 575, 582 , 589, 666, 673 , 680, 414, 505, 596, 687, 421, 512, 603, 694, 428 , 435 519 , 526 610 , 617 701 , 708 568, 575, 582, 589, 596, 603, 610, 617 , 624, 631, 638, 645 , 652 659, 666, 673, 680, 687, 694, 701, 708 , 715, 722, 729, 732 526 533 621 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 80 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M2 (CONTINUED FROM PREVIOUS PAGE) C92626 T 105 EYES SCAB EYE-RIGHT SKIN & PELAGE SORE/SCAB PAW-HIND-LEFT C92627 2 C92628 M NORMAL NO REMARKABLE OBSERVATIONS 14 NORMAL NO REMARKABLE OBSERVATIONS 73 APPEARANCE THIN EXCRETION FEW FECES SKIN & PELAGE ROUGH HAIR COAT YELLOW HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS DOSE: 0.5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 316 526, 533, 54 0,. 547, 554, 561, 568,. 575, 582, 589, 596 , 603, 610 617, 624, 631,. 638, 645, 652, 659,. 666, 673, 680, 687 , 694, 701 708, 715, 722,. 729, 732 1 , 8 ,r 15, 2 2 , 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99 1 , 8 ,r 15, 2 2 , 29, 36, 43, 50, 57, 64, 71, 78, 85, 92- 93 428, 435, 442,. 449, 456, 463, 470,. 477, 484, 491, 498 , 505-506 372, 428, 435, 442 456, 463, 470,. 477, 484, 491, 498, 505-506 372 1 , 8 ,r 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 379 , 386 393, 400, 407, 414 , 421 622 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 81 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M2 DOSE: 0.5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92629 D 78 APPEARANCE THIN EXCRETION FEW FECES NORMAL NO REMARKABLE OBSERVATIONS M 88 APPEARANCE THIN BEHAVIOR ATAXIC <UNSPECIFIED> HYPOACTIVE <UNSPECIFIED> EYES CLEAR DISCHARGE EYES RED DISCHARGE EYES NORMAL NO REMARKABLE OBSERVATIONS 540, 546 540, 546 1 , 8 ,r 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127, 134 , 141, 148, 155, 162 , 169, 176, 183,. 190, 197 204, 2 1 1 , 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281, 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365,. 372, 379 386, 393, 400, 407 , 414, 421, 428, 435 , 442, 449, 456,. 463, 470 477, 484, 491, 498 , 505, 512, 519, 526 , 533 589, 596, 603, 610 , 616 616 616 603, 610 616 1 , 8 ,r 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127, 134 , 141, 148, 155, 162 , 169, 176, 183,. 190, 197 623 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 82 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M2 (CONTINUED FROM PREVIOUS PAGE) C92630 M 88 NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 73 DOSE: 0.5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 204, 295, 386, 477, 211, 218, 302, 309, 393, 400, 484, 491, 225 , 232 , 239, 316 , 323 , 330, 407 , 414 , 421, 498 246, 337, 428, 253, 344, 435, 260, 351, 442, 267, 358, 449, 274 , 281, 365 , 372, 456 , 463, 288 379 470 505, 512C, 519C, 526C, 533C, 54 0C, 547C, 554C , 561C, 568C, 575C 582C, 589C , 596C, 603C, 610C, 616C 624 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 73 MASS(ES) 74 DORSAL WEEK 73 75 MASS(ES) 78 DORSAL WEEK 73 79 MASS(ES) DORSAL WEEK 73 80 MASS(ES) 81 DORSAL WEEK 73 82 MASS(ES) 84 DORSAL WEEK 73 85 MASS(ES) 86 DORSAL WEEK 73 87 MASS(ES) 89 DORSAL WEEK 73 90 MASS(ES) 97 DORSAL WEEK 73 10 X 10MM 15 X 15MM 20 X 15MM 20 X 20MM 20 X 25MM 20 X 30MM 25 X 30MM 30 X 40MM 625 Covance 6329-183 3M T-6295 PAGE: 83 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT M2 701 101 MASS(ES) AXILLARY REGION-LEFT WEEK 101 708 102 MASS(ES) TO 733 105 AXILLARY REGION-LEFT WEEK 101 477 69 TO 505 73 MASS(ES) DORSAL WEEK 69 512 74 TO 526 76 MASS(ES) DORSAL WEEK 69 533 77 TO 561 81 MASS(ES) DORSAL WEEK 69 568 82 TO 673 97 MASS(ES) DORSAL WEEK 69 680 98 MASS(ES) TO 722 104 DORSAL WEEK 69 729 105 MASS(ES) TO 733 105 DORSAL WEEK 69 30 X 30MM 30 X 40MM 10 X 10MM 15 X 15MM 20 X 20MM 20 X 25MM 25 X 25MM 30 X 30MM 626 Covance 6329-183 3M T-6295 PAGE: 84 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C92580 C92581 M2 435 63 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 61 442 64 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 61 M2 589 85 TO 617 89 MASS(ES) DORSAL WEEK 85 645 93 MASS(ES) TO 729 105 LIMB-HIND-RIGHT WEEK 93 344 50 TO 372 54 MASS(ES) SHOULDER-RIGHT WEEK 50 379 55 TO 386 56 MASS(ES) SHOULDER-RIGHT WEEK 50 393 57 TO 505 73 MASS(ES) SHOULDER-RIGHT WEEK 50 512 74 TO 547 79 MASS(ES) SHOULDER-RIGHT WEEK 50 10 X 10MM 15 X 15MM 10 X 15MM 10 X 10MM 10 X 10MM 15 X 15MM 20 X 20MM 15 X 15MM 627 Covance 6329-183 3M T-6295 PAGE: 85 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C92582 C92583 C92585 C92591 M2 680 98 TO 687 99 MASS(ES) SHOULDER-LEFT WEEK 98 694 100 MASS(ES) TO 702 101 SHOULDER-LEFT WEEK 98 M2 512 74 TO 673 97 MASS(ES) DORSAL WEEK 74 680 98 MASS(ES) TO 731 105 DORSAL WEEK 74 M2 267 39 TO 268 39 MASS(ES) AXILLARY REGION-LEFT WEEK 39 260 38 TO 268 39 MASS(ES) AXILLARY REGION-RIGHT WEEK 37 253 37 TO 268 39 MASS(ES) VENTRAL-CERVICAL WEEK 37 M2 582 84 TO 610 CO CO MASS(ES) VENTRAL-ABDOMINAL WEEK 79 40 X 40MM 60 X 40MM 10 X 10MM 15 X 15MM 10 X 10MM 20 X 20MM 30 X 20MM 10 X 10MM 628 Covance 6329-183 3M T-6295 PAGE: 86 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C92591 C92595 C92598 C92601 M2 659 95 TO 670 96 MASS(ES) VENTRAL -ABDOMINAL-LEFT WEEK 95 498 72 TO 512 74 MASS(ES) VENTRAL -ABDOMINAL-RIGHT WEEK 72 M2 505 73 MASS(ES) DORSAL WEEK 73 512 74 TO 526 76 MASS(ES) DORSAL WEEK 73 533 77 TO 561 81 MASS(ES) DORSAL WEEK 73 568 82 TO 583 84 MASS(ES) DORSAL WEEK 73 M2 715 103 MASS(ES) AXILLARY REGION-RIGHT WEEK 103 M2 498 72 MASS(ES) TO 715 103 DORSAL WEEK 70 10 X 10MM 25 X 25MM 10 X 10MM 15 X 15MM 25 X 25MM 25 X 30MM 10 X 10MM 10 X 10MM 629 Covance 6329-183 3M T-6295 PAGE: 87 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 73 MASS(ES) 76 VENTRAL-ABDOMINAL-LEFT WEEK 73 83 MASS(ES) 84 VENTRAL-ABDOMINAL-LEFT WEEK 83 87 MASS(ES) 104 VENTRAL-ABDOMINAL-LEFT WEEK 87 74 MASS(ES) 78 SHOULDER-RIGHT WEEK 66 28 MASS(ES) 29 INGUINAL REGION-RIGHT WEEK 28 102 MASS(ES) 104 DORSAL WEEK 102 98 WART LIKE LESION(S) 104 HEAD-MAXILLARY-LEFT WEEK 98 72 MASS(ES) 73 VENTRAL-ABDOMINAL-RIGHT WEEK 72 10 X 10MM 30 X 30MM 40 X 50MM 10 X 10MM 10 X 10MM 10 X 10MM CORNER OF LEFT EYE 15 X 15MM 630 Covance 6329-183 3M T-6295 PAGE: 88 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 74 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 72 75 MASS(ES) 77 VENTRAL-ABDOMINAL-RIGHT WEEK 72 78 MASS(ES) 79 VENTRAL-ABDOMINAL-RIGHT WEEK 72 80 MASS(ES) 81 VENTRAL-ABDOMINAL-RIGHT WEEK 72 82 MASS(ES) 83 VENTRAL-ABDOMINAL-RIGHT WEEK 72 84 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 72 85 MASS(ES) 86 VENTRAL-ABDOMINAL-RIGHT WEEK 85 87 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 85 20 X 20MM 25 X 25MM 45 X 40MM 50 X 50MM 60 X 60MM 75 X 80MM 80 X 80MM 100 X 100MM 631 Covance 6329-183 3M T-6295 PAGE: 89 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C92611 C92614 C92619 C92620 C92630 M2 610 88 TO 610 88 M2 617 89 TO 631 91 638 92 TO 640 92 M2 442 64 M2 491 71 TO 498 72 M2 512 74 TO 616 88 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 85 MASS(ES) DORSAL WEEK 89 MASS(ES) DORSAL WEEK 89 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 64 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 71 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 73 120 X 120MM 10 X 10MM 15 X 15MM 30 X 10MM 20 X 20MM 10 X 10MM Covance 6329-183 3M T-6295 PAGE: 90 632 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 91 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M3 DOSE: 2 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92631 T 105 NORMAL NO REMARKABLE OBSERVATIONS C92632 M 103 APPEARANCE THIN BEHAVIOR COLD TO TOUCH ENTIRE BODY EXCRETION FEW FECES RESPIRATION LABORED SKIN & PELAGE SORE/SCAB EAR-LEFT NORMAL NO REMARKABLE OBSERVATIONS 1, 8 15, 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582, 589, 596, 603, 610, 617 , 624, 631, 638, 645 , 652 659, 666, 673, 680, 687, 694, 701, 708 , 715, 722, 729, 731 715 715 715 715 393, 400, 407,. 414, 421, 428, 435, 442 , 449, 456, 463, 470 , 477 484, 491, 498, 505, 512, 519, 526, 533 , 540, 547, 554, 561 , 568 575, 582, 589,. 596, 603, 610, 617, 624 , 631, 638, 645, 652 , 659 666, 673, 680, 687, 694, 701, 708, 715 1 , 8 ,r 15, 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 633 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 92 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M3 (CONTINUED FROM PREVIOUS PAGE) C92632 M 103 NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) EAR-LEFT WEEK 48 C92633 T VENTRAL-CERVICAL-LEFT WEEK 103 105 APPEARANCE MALOCCLUSION DISCHARGE NASAL RED IN COLOR UNKNOWN RED IN COLOR RESPIRATION AUDIBLE DOSE: 2 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 113, 1 2 0, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323 330, 337, 344, 351 , 358, 365, 421C, 428C , 435C, 442C, 44 9C, 498C, 505C , 512C, 519C, 526C, 575C, 582C , 589C, 596C, 603C, 652C, 659C , 666C, 673C, 680C, 372, 379, 386, 393 , 400, 407, 456C, 463C, 470C, 477C, 484C, 533C, 54 0C, 54 7C, 554C, 561C, 610C, 617C, 624C, 631C, 638C, 687C, 694C, 701C, 708C, 715C 414 491C 568C 645C 715C 330, 337, 344, 351, 365, 372, 379, 386, 393, 400, 407, 428, 435 442, 449, 456, 463, 470, 477, 484, 540, 547, 554, 561, 568, 575 582, 624, 631, 638, 645, 652, 659, 666, 673, 680, 687, 694, 701 708, 715, 722, 729, 732 22 21 21 634 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 93 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M3 (CONTINUED FROM PREVIOUS PAGE) C92633 T 105 SKIN & PELAGE SORE/SCAB PAW-HIND-RIGHT NORMAL NO REMARKABLE OBSERVATIONS D 87 NORMAL NO REMARKABLE OBSERVATIONS C92635 M 79 BEHAVIOR HYPOACTIVE <UNSPECIFIED> EXCRETION FEW FECES NONFORMED FECES SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE DOSE: 2 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 722, 729, 732 1 , 8 ,r 15, 29, 36, 43 , 50,r 57, 64, 71, 78, 85,r 92, 99, 106, 113 1 2 0, 127, 134, 141, 148, 155, 162, 169 , 176, 183, 190, 197 , 204 2 1 1 , 218, 225,. 232, 239, 246, 253, 260 , 267, 274, 281, 288 , 295 302, 309, 316,. 323, 358, 414, 421, 491 , 498, 505, 512, 519 , 526 533, 589, 596, 603, 610, 617 1 , 8 ,r 15, 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582, 589, 596, 603 549 547, 549 549 337, 344, 351, 358, 365, 372, 379, 386, 393, 400, 407, 414, 421 428, 435, 442, 449, 456, 463, 470, 519, 526, 533, 540, 547, 549 635 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 94 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M3 (CONTINUED FROM PREVIOUS PAGE) C92635 M 79 SKIN & PELAGE ROUGH HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS C92636 D MASS(ES) HEAD-MAXILLARY-RIGHT WEEK 76 98 APPEARANCE SWOLLEN TESTES SKIN & PELAGE SORE/SCAB VENTRAL-ABDOMINAL VENTRAL-ABDOMINAL-LEFT NORMAL NO REMARKABLE OBSERVATIONS DOSE: 2 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 533, 540 1 , 8 ,r 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 477, 484 , 491, 498, 505, 512 526C, 533C, 540C, 547C, 549C 309, 400, 491, 582, 673, 316, 407, 498, 589, 680, 323, 414, 505, 596, 682 330, 421, 512, 603, 337, 428, 519, 610, 344, 435, 526, 617, 351, 442, 533, 624, 358, 449, 540, 631, 365, 456, 547, 638, 372, 463, 554, 645, 379, 470, 561, 652, 386, 477, 568, 659, 393 484 575 666 400 617, 624, 631, 638 , 645, 652, 659, 666 , 673, 680, 682 1 , 8 ,r 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190, 197 204, 2 1 1 , 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281, 288 295, 302 636 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 95 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M3 (CONTINUED FROM PREVIOUS PAGE) C92636 D 98 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 88 C92637 T 105 NORMAL NO REMARKABLE OBSERVATIONS C92638 M 91 APPEARANCE THIN SKIN & PELAGE PALE ENTIRE BODY NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 73 DOSE: 2 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 610 1 22, 8,r 15, , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 120113, , 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 211204, , 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 666568, 575, 582, 589, 596, 603, 610, 617 , 624, 631, 638, 645 , 652 659, , 673, 680, 687, 694, 701, 708 , 715, 722, 729, 731 589, 596, 603,. 610, 617, 624, 631 610, 617, 624, 631 1 22, 8,r 15, , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 120113, , 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 211204, , 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 526, 533, 540, 547 , 554, 561, 568, 575 , 582 505, 512, 519 637 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 96 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M3 DOSE: 2 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92639 M 85 APPEARANCE HUNCHED THIN EXCRETION FEW FECES SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE NORMAL NO REMARKABLE OBSERVATIONS C92640 2 MASS(ES) TAIL WEEK 49 14 NORMAL NO REMARKABLE OBSERVATIONS 589 512, 519, 512, 589 526, 533, 540, 547, 582,589 512, 519, 52 6 , 533 , 540, 547 1 , 8 ,r 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 344, 351 , 358, 365, 372, 379 , 386 393, 400, 407, 414 , 421, 428, 435, 442 , 449, 456, 463, 470 , 477 484, 491, 498, 505 , 554, 561, 568, 575 337 1, 8 , 15, 22, 29, 36, 43, 57, 64, 71, 78, 85, 92-93 638 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 97 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M3 DOSE: 2 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92641 T 105 APPEARANCE MALOCCLUSION C92642 T MISSING TEETH NORMAL NO REMARKABLE OBSERVATIONS NORMAL NO REMARKABLE OBSERVATIONS C92643 T 105 APPEARANCE MALOCCLUSION SKIN & PELAGE SORE/SCAB VENTRAL-ABDOMINAL-RIGHT 176, 267, 386, 477, 568, 659, 183, 19 0, 274, 281, 393, 400, 484, 491, 575, 582, 666, 673, 197 , 204, 288 , 295, 407 , 414, 498 , 505, 589 , 596, 680 , 687, 2 1 1 , 218, 302, 309, 421, 428, 512, 519, 603, 610, 694, 701, 225 , 232, 316 , 323, 435 , 442, 526 , 533, 617 , 624, 708 , 715, 239, 330, 449, 540, 631, 722, 246, 337, 456, 547, 638, 729, 253 , 260 344 , 351 463 , 470 554 , 561 645 , 652 732 687, 694, 701, 708 , 715, 722, 729, 732 1 , 8 ,r 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127, 134 , 141, 148, 155, 162 , 169, 358, 365, 372 , 379 1 , 8 ,r 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407 , 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498 , 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582, 589 , 596, 603, 610, 617 , 624, 631, 638, 645 , 652 659, 666, 673, 680 , 687, 694, 701, 708 , 715, 722, 729, 731 554, 666, 673 372, 379 639 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 98 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M3 (CONTINUED FROM PREVIOUS PAGE) C92643 T 105 NORMAL NO REMARKABLE OBSERVATIONS C92644 T MASS(ES) HEAD-MAXILLARY-LEFT WEEK 73 VENTRAL-ABDOMINAL-RIGHT WEEK 101 VENTRAL-THORACIC-RIGHT WEEK 101 105 NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) DORSAL WEEK 104 DOSE: 2 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 1, 8 15, 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 386 , 393 400, 407, 414, 421, 428, 435, 442, 449 , 456, 463, 470, 477 , 484 491, 498, 526,. 533, 540, 547, 561, 568 , 575, 582, 589, 596 , 603 610, 617, 624, 631, 638, 645, 652, 659 , 680, 687, 694 505, 512, 519 701C 708C 715C, 722C, 729C, 733C 1, 8 15, 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582, 589, 596, 603, 610, 617 , 624, 631, 638, 645 , 652 659, 666, 673, 680, 687, 694, 701, 708 , 715 722, 729, 731 640 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 99 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M3 DOSE: 2 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92645 2 C92646 1 C92647 M 14 EYES RED DISCHARGE EYE-LEFT NORMAL NO REMARKABLE OBSERVATIONS 4 NORMAL NO REMARKABLE OBSERVATIONS 80 BEHAVIOR HYPOACTIVE <UNSPECIFIED> EXCRETION FEW FECES NO FECES EYES SQUINTED PERI-ORBITAL SKIN & PELAGE ROUGH HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS 64 1, 8 , 15, 22, 29, 36, 43, 50, 57, 71, 78, 85, 92-93 1, 8 , 15, 22 557 551 557 557 554, 557 1 , 8 ,r 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400 407 , 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491 498 , 505, 512, 519, 526 , 533, 540, 547 641 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 100 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M3 DOSE: 2 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92648 M 87 APPEARANCE THIN BEHAVIOR AGGRESSIVE <UNSPECIFIED> HYPOACTIVE <UNSPECIFIED> EXCRETION FEW FECES SKIN & PELAGE SORE/SCAB VENTRAL-ABDOMINAL-LEFT NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 62 589, 596, 599, 603,605 589, 596 605 605 463, 470 1 , 8 ,r 15, 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 477, 484 , 491, 498, 505, 512 , 519 526, 533, 540, 547, 554, 561, 568, 575 , 582 428, 435, 442C, 449C, 456C 642 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 101 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M3 DOSE: 2 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92649 T 105 SKIN & PELAGE SORE/SCAB VENTRAL-ABDOMINAL VENTRAL-ABDOMINAL-RIGHT NORMAL NO REMARKABLE OBSERVATIONS C92650 1 C92651 M 4 NORMAL NO REMARKABLE OBSERVATIONS 81 APPEARANCE SWOLLEN PAW-HIND-RIGHT TAIL-PROXIMAL THIN BEHAVIOR HYPOACTIVE <UNSPECIFIED> EXCRETION FEW FECES 722 428, 435, 442,. 624, 631, 638 1 , 8 ,r 15, 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 449, 456 , 463, 470, 477, 484 , 491 498, 505, 512, 519, 526, 533, 540, 547 , 554, 561, 568, 575 , 582 589, 596, 603,. 610, 617, 645, 652, 659 , 666, 673, 680, 687 , 694 701, 708, 715, 729, 732 1, 8 , 15, 22 533, 540, 547, 554, 561 540, 547, 554, 561 547, 554, 561 561 561 643 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 102 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M3 (CONTINUED FROM PREVIOUS PAGE) C92651 M 81 EYES SQUINTED PERI-ORBITAL NORMAL NO REMARKABLE OBSERVATIONS 1 C92653 T 4 NORMAL NO REMARKABLE OBSERVATIONS 105 SKIN & PELAGE SORE/SCAB VENTRAL-ABDOMINAL NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) VENTRAL-ABDOMINAL WEEK 88 DOSE: 2 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 561 1 , 8 ,r 15, 2 2 , 29, 36, 43,r 50, 113, 1 2 0 , 127, 134 , 141, 148, 204, 2 1 1 , 218, 225 , 232, 239, 295, 302, 309, 316 , 323, 330, 386, 393, 400, 407 , 414, 421, 477, 484, 491, 498 , 505, 512, 57, 64, 71, 78, 85, 155, 162 , 169, 176, 246, 253 , 260, 267, 337, 344 , 351, 358, 428, 435 , 442, 449, 519, 526 92, 99, 106 183, 190 , 197 274, 281 , 288 365, 372 , 379 456, 463 , 470 1 , 8 ,r 15, 22 610, 617, 624, 631, 638, 645 1 , 8 ,r 15, 2 2 , 29, 36 , 43,r 50, 113, 1 2 0 , 127,. 134, 141, 148, 204, 2 1 1 , 218, 225, 232, 239, 295, 302, 309,. 316, 323, 330, 386, 393, 400, 407, 414, 421, 477, 484, 491, 498, 505, 512, 568, 575, 582, 589, 596, 603, 701, 708, 715,. 722, 729, 733 57, 64, 71, 78, 85, 155, 162 , 169, 176, 246, 253 , 260, 267, 337, 344 , 351, 358, 428, 435 , 442, 449, 519, 526 , 533, 540, 652, 659 , 666, 673, 92, 99, 106 183, 190 , 197 274, 281 , 288 365, 372 , 379 456, 463 , 470 547, 554 , 561 680, 687 , 694 610C, 617C 644 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 103 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M3 DOSE: 2 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92654 M 79 EXCRETION NO FECES EYES CLEAR DISCHARGE EYE-LEFT OPAQUE EYE-LEFT PROTRUDING EYE-LEFT SCAB EYE-LEFT SKIN & PELAGE SORE/SCAB HEAD-CRANIAL NORMAL NO REMARKABLE OBSERVATIONS 549 519 526, 533, 540, 547, 549 549 533, 540, 547, 549 526 1 , 8 ,r 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407 , 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498 , 505, 512 645 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 104 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M3 DOSE: 2 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92655 M 94 SKIN & PELAGE SORE/SCAB TAIL-MID NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) TAIL-MID WEEK 85 M 97 APPEARANCE THIN EXCRETION FEW FECES SKIN & PELAGE PALE ENTIRE BODY SORE/SCAB DORSAL-THORACIC 624, 631, 638, 645, 652-653 1 , 8 , 15, 2 2 , 29, 36, 43 , 50, 57, 64, 71 , 78, 85 , 92, 99, 106 113, 1 2 0 , 127, 134 , 141, 148, 155, 162, 169, 176 , 183, 190 , 197 204, 2 1 1 , 218, 225 , 232, 239, 246, 253, 260, 267 , 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344, 351, 358 , 365, 372 , 379 386, 393, 400, 407 , 414, 421, 428, 435, 442, 449 , 456, 463 , 470 477, 484, 491, 498 , 505, 512, 519, 526, 533, 540 , 547, 554 , 561 568, 575, 582 589C, 596C , 603C, 610C, 617C, 624C , 631C , 638C, 645C, 652- 653C 659, 666, 673 645, 652, 673 673 414, 421, 428, 435 , 442, 449, 456, 463, 470, 477 , 484, 491 , 498 505, 512, 519, 526 , 533, 540, 547, 554, 561, 568 , 575, 582 , 589 596, 603, 610, 617 , 624, 631, 638, 645, 652, 659 , 666, 673 646 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 105 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M3 (CONTINUED FROM PREVIOUS PAGE) C92656 M 97 NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) DORSAL WEEK 49 C92657 T 105 SKIN & PELAGE SORE/SCAB DORSAL-CERVICAL-LEFT NORMAL NO REMARKABLE OBSERVATIONS ASS(ES) DORSAL WEEK 93 HEAD-MAXILLARY-RIGHT WEEK 91 DOSE: 2 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 1 , 8 ,. 15, 2 2 , 29, 36, 43 , 50, 57, 64, 71, 78 , 85, 92, 99, 106 113, 1 2 0 , 127,. 134, 141, 148, 155, 162, 169, 176, 183,, 190, 197 204, 2 1 1 , 218,. 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309,. 316, 323, 330 337, 344, 351, 358C, 365C, 372C, 379C, 386C, 393C, 400C, 407C 414C, 421C , 428C, 435C, 442C, 449C , 456C, 46 3C, 470C, 477C, 484C 491C, 498C , 505C, 512C, 519C, 526C , 533C, 54 0C, 547C, 554C, 561C 568C, 575C , 582C 596 1 , 8 , 15, 2 2 , 29, 36, 43 , 50, 57, 64, 71 , 78, 85 , 92, 99, 106 113, 1 2 0 , 127,. 134, 141, 148, 155, 162, 169, 176 , 183, 190 , 197 204, 2 1 1 , 218,. 225, 232, 239, 246, 253, 260, 267 , 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344, 351, 358 , 365, 372 , 379 386, 393, 400,. 407, 414, 421, 428, 435, 442, 449 , 456, 463 , 470 477, 484, 491, 498, 505, 512, 561, 568, 575, 582 , 589, 603 , 610 617 645C, 652C , 659C, 666C, 673C, 680C , 687C , 694C, 701C, 708C , 715C 722C 631, 638, 645 647 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 106 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M3 (CONTINUED FROM PREVIOUS PAGE) C92657 T 105 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 75 WART LIKE LESION(S) SHOULDER-LEFT WEEK 90 C92658 D 94 EYES RED DISCHARGE EYE-RIGHT NORMAL NO REMARKABLE OBSERVATIONS T APPEARANCE MALOCCLUSION SWOLLEN PERI-ORAL PAW-HIND-LEFT NORMAL NO REMARKABLE OBSERVATIONS DOSE: 2 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 519, 526, 533,. 540, 547, 554 624, 631, 638, 645, 652, 659, 666, 673 , 680, 687, 694, 701 , 708 715, 722, 729, 733 2 1 1 , 218, 302, 309, 393, 400, 484, 491, 624, 631, 225,. 232, 316,. 323, 407,. 414, 498, 505, 638, 645, 239, 246, 330, 337, 421, 428, 512, 519, 652- 653 253, 344, 435, 526, 260 , 267, 351 , 358, 442 , 449, 533 , 540, 274, 365, 456, 547, 281, 372, 463, 603, 288 , 295 379 , 386 470 , 477 610 , 617 1 , 8 ,r 15, 2 2 , 29, 36 , 43 , 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 554, 561,. 568, 575, 582, 589, 596 407, 456, 463,. 554, 561, 568, 575, 582 , 589, 596, 603, 652 , 659 666, 673, 680, 687, 694, 701, 708, 715 , 722, 729, 732 652, 659, 666 561, 568, 575,. 582, 589, 596, 603, 610 , 617, 624, 631, 638 , 645 652, 659, 666,. 673, 680, 687, 694, 701 , 708, 715, 722, 729 , 732 1 , 8 ,r 15, 2 2 , 29, 36 , 43 , 50, 57, 64, 71, 78, 85, 92, 99, 106 648 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 107 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M3 (CONTINUED FROM PREVIOUS PAGE) C92659 T 105 NORMAL NO REMARKABLE OBSERVATIONS C92660 T 105 APPEARANCE MALOCCLUSION NORMAL NO REMARKABLE OBSERVATIONS C92661 M 90 APPEARANCE THIN EXCRETION NO FECES SKIN & PELAGE PALE ENTIRE BODY NORMAL NO REMARKABLE OBSERVATIONS DOSE: 2 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 113, 204, 295, 386, 498, 12 0 , 127, 2 1 1 , 218, 302, 309, 393, 400, 505, 512, 134, 225, 316, 414, 519, 141, 232, 323, 421, 526, 148, 239, 330, 428, 533, 155, 246, 337, 435, 540, 162, 253, 344, 442, 547 169, 260, 351, 449, 176, 267, 358, 470, 183, 274, 365, 477, 190, 281, 372, 484, 197 288 379 491 463, 470, 477, 484, 533, 561, 568, 575, 582, 589, 596, 603, 610 659, 666, 673, 680, 708 1 , 8 ,r 15, 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 491 , 498 505, 512, 519,. 526, 540, 547, 554, 617 , 624, 631, 638, 645 , 652 687, 694, 701,. 715, 722, 729, 732 589, 596, 603,. 610, 617, 624 589 624 1, 8 , 15, 22, 29, 36, 43, 57, 64, 71, 78, 85, 92, 99, 106 649 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 108 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M3 (CONTINUED FROM PREVIOUS PAGE) C92661 M 90 NORMAL NO REMARKABLE OBSERVATIONS WART LIKE LESION(S) VENTRAL-THORACIC-LEFT WEEK 46 C92662 2 C92663 D 14 NORMAL NO REMARKABLE OBSERVATIONS 95 DISCHARGE GENITAL RED IN COLOR EXCRETION DISCOLORED URINE RED IN COLOR NORMAL NO REMARKABLE OBSERVATIONS DOSE: 2 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 120113, , 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 211204, , 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309 316, 323, 330, 337, 344, 351, 358, 365, 372, 379, 386, 393, 400 407, 414, 421, 428, 435, 442, 449, 456, 463, 470, 477, 484, 491 498, 505, 512, 519, 526, 533, 540, 547, 554, 561, 568, 575, 582 1, 8, 15, 22, 29, 36, 43, 50 57, 64, 71, 78 85 92-93 379 400, 498, 504 1, 8, 15 113, 120 204, 211 295, 302 393, 407 491, 505 589, 596 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 386 414, 421, 428, 435, 442, 449 , 456, 463, 470, 477 , 484 512, 519, 526, 533, 540, 547 , 554, 561, 568, 575 , 582 603, 610 650 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 109 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M3 (CONTINUED FROM PREVIOUS PAGE) C92663 D 95 MASS(ES) SHOULDER-LEFT WEEK 89 C92664 D 78 APPEARANCE THIN BEHAVIOR HYPOACTIVE <UNSPECIFIED> RECUMBENT LATERAL RESPIRATION LABORED SKIN & PELAGE ROUGH HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS DOSE: 2 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 617C, 624C, 631C, 638C, 645C, 652C, 659-660C 533, 540 540 540 540 533, 540 1 , 8 ,r 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400 407 , 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491 498 , 505, 512, 519, 526 651 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 110 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M3 DOSE: 2 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92665 D 88 APPEARANCE SWOLLEN PAW-HIND-RIGHT NORMAL NO REMARKABLE OBSERVATIONS M 76 APPEARANCE HEAD TILT RIGHT THIN BEHAVIOR HYPOACTIVE <UNSPECIFIED> EXCRETION FEW FECES SKIN & PELAGE ROUGH HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS 575, 582, 589,. 596, 603, 610, 614 1 , 8 ,r 15, 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568 526, 530 512, 519, 526, 530 530 512, 530 526, 530 1 , 8 ,r 15, 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 652 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 111 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M3 (CONTINUED FROM PREVIOUS PAGE) C92666 M 76 NORMAL NO REMARKABLE OBSERVATIONS C92667 M 79 APPEARANCE THIN BEHAVIOR HYPOACTIVE <UNSPECIFIED> EXCRETION FEW FECES EYES RED DISCHARGE EYE-LEFT EYE-RIGHT EYES SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE NORMAL NO REMARKABLE OBSERVATIONS DOSE: 2 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470 477, 484, 491, 498, 505 547, 549 549 526, 528, 533, 540, 547, 549 43 512, 519 526, 528, 533, 540 528, 533, 54 0, 547 , 549 1 , 8 ,r 15, 2 2 , 29, 36, 50,r 57, 64, 71, 78, 85,, 92, 99, 106, 113 1 2 0 , 127, 134, 141 , 148, 155, 162, 169 , 176, 183, 190, 197, 204 2 1 1 , 218, 225, 232 , 239, 246, 253, 260 , 267, 274, 281,. 288, 295 302, 309, 316, 323 , 330, 337, 344, 351 , 358, 365, 372, 379, 386 393, 400, 407, 414 , 421, 428, 435, 442 , 449, 456, 463, 470 653 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 112 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M3 (CONTINUED FROM PREVIOUS PAGE) C92667 M 79 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 69 C92668 1 C92669 T 4 NORMAL NO REMARKABLE OBSERVATIONS 105 NORMAL NO REMARKABLE OBSERVATIONS C92670 M 74 APPEARANCE HUNCHED THIN EXCRETION NONFORMED FECES SKIN & PELAGE PALE ENTIRE BODY ROUGH HAIR COAT SORE/SCAB LATERAL-ABDOMINAL-LEFT LATERAL-ABDOMINAL-RIGHT DOSE: 2 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 477C, 484C, 491C, 498C, 505C, 512C, 519C, 526C, 528C, 533C, 540C 54 7C, 549C 1, 8 , 15, 22 1, 8 15, 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582, 589, 596, 603, 610, 617 , 624, 631, 638, 645 , 652 659, 666, 673, 680, 687, 694, 701, 708 , 715, 722, 729, 732 512 498, 505, 512 456 512 449, 456, 463, 470, 477, 484, 491, 498, 505, 512 449, 456, 463, 470, 477 449, 456, 463, 470, 477, 484 654 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 113 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M3 (CONTINUED FROM PREVIOUS PAGE) C92670 M 74 SKIN & PELAGE SORE/SCAB LATERAL-CERVICAL-RIGHT SHOULDER-LEFT SHOULDER-RIGHT NORMAL NO REMARKABLE OBSERVATIONS D 93 APPEARANCE MALOCCLUSION DISCHARGE UNKNOWN RED IN COLOR EYES RED DISCHARGE EYE-LEFT SKIN & PELAGE SORE/SCAB VENTRAL-ABDOMINAL DOSE: 2 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 484, 491, 498, 505 449, 456 449, 456 1 , 8 ,r 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407 , 414, 421, 428, 435 , 442 141, 232, 323, 414, 505, 596, 148, 239, 330, 421, 512, 603, 155, 246, 337, 428, 519, 610, 162 , 169, 253 , 260, 344 , 351, 435 , 442, 526 , 533, 617 , 624, 176, 267, 358, 449, 540, 631, 183, 274, 365, 456, 547, 638, 190 , 197, 281 , 288, 372 , 379, 463 , 470, 554 , 561, 645 , 650 204, 295, 386, 477, 568, 2 1 1 , 218 , 225 302, 309 , 316 393, 400 , 407 484, 491 , 498 575, 582 , 589 45 50, 57, 64, 71, 78, 85, 92, 99, 106, 113, 120, 127, 134 218, 225 655 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 114 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M3 (CONTINUED FROM PREVIOUS PAGE) C92671 D 93 NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) VENTRAL-ABDOMINAL WEEK 29 C92672 M 95 BEHAVIOR HYPOACTIVE <UNSPECIFIED> EXCRETION FEW FECES EYES RED DISCHARGE EYE-LEFT NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) DORSAL-CERVICAL-LEFT WEEK 95 DOSE: 2 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 1, 8 , 15, 22, 29, 36, 43 197C, 204C, 211C 665 656, 659, 665 665 1 , 8 ,r 15, 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582, 589, 596, 603, 610, 617 , 624, 631, 638, 645 , 652 659, 665 656 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 115 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M3 DOSE: 2 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92673 D 95 NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) AXILLARY REGION-LEFT WEEK 81 C92674 T WART LIKE LESION(S) LIMB-FRONT-RIGHT WEEK 65 105 SKIN & PELAGE SORE/SCAB VENTRAL-ABDOMINAL-RIGHT NORMAL NO REMARKABLE OBSERVATIONS 1 , 8 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407 , 414, 421, 428, 435 , 442, 477, 484, 491 , 498 505, 512, 519, 526 , 533, 540, 547, 554 561, 56 8C, 575C, 582C, 589C, 596C, 603C, 610C, 617C, 624C, 631C 638C 64 5C, 652C, 659C 449, 456, 463, 470 400, 407 1 , 8 ,r 15, 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 414, 421, 428, 435, 442, 449, 456, 463 , 470, 477, 484, 491 , 498 505, 512, 519,. 526, 533, 540, 547, 554 , 561, 568, 575, 582 , 589 596, 603, 610, 617, 624, 631, 638, 645 , 652, 659, 666, 673 , 680 687, 694, 701,. 708, 715, 722, 729, 731 657 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 116 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M3 (CONTINUED FROM PREVIOUS PAGE) C92674 T 105 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 56 C92675 M 102 APPEARANCE THIN EYES RED DISCHARGE EYE-LEFT NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) DORSAL WEEK 90 DOSE: 2 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 386C, 393C 708, 712 680, 687, 694, 701 , 708 , 712 1 , 8 , 15, 2 2 , 29, 36, 43, 50 , 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127, 134 , 141 , 148 , 155, 162 , 169, 176, 183, 190, 197 204, 2 1 1 , 218, 225 , 232 , 239 , 246, 253 , 260, 267, 274, 281, 288 295, 302, 309, 316 , 323 , 330 , 337, 344 , 351, 358, 365, 372, 379 386, 393, 400, 407 , 414 , 421 , 428, 435 , 442, 449, 456, 463, 470 477, 484, 491, 498 , 505 , 512 , 519, 526 , 533, 540, 547, 554, 561 568, 575, 582, 589 , 596 , 603 , 610, 617 624, 631, 638C, 64 5C, 652C, 659C, 666C , 673C, 680C , 687C, 694C 701C, 708C , 712C 658 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 117 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M3 DOSE: 2 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92676 2 C92677 D 14 NORMAL NO REMARKABLE OBSERVATIONS 99 NORMAL NO REMARKABLE OBSERVATIONS C92678 1 C92679 M WART LIKE LESION(S) DORSAL WEEK 80 4 NORMAL NO REMARKABLE OBSERVATIONS 55 BEHAVIOR HYPOACTIVE <UNSPECIFIED> EXCRETION FEW FECES EYES RED DISCHARGE EYES RESPIRATION IRREGULAR 1 , 8 ,r 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92- 93 1 , 8 ,r 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127, 134 , 141, 148, 155, 162 , 169, 176, 183 , 190 , 197 204, 2 1 1 , 218, 225 , 232, 239, 246, 253 , 260, 267, 274 , 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365 , 372 , 379 386, 393, 400, 407 , 414, 421, 428, 435 , 442, 449, 456 , 463 , 470 477, 484, 491, 498 , 505, 512, 519, 526 , 533, 540, 547 554, 561, 568, 575, 582, 589, 596, 603, 610, 617, 624, 631, 638 645, 652, 659, 666, 673, 680, 687, 690 1, 8 , 15, 22 659 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 118 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M3 (CONTINUED FROM PREVIOUS PAGE) C92679 M 55 NORMAL NO REMARKABLE OBSERVATIONS C92680 M 84 APPEARANCE HUNCHED THIN BEHAVIOR HYPOACTIVE <UNSPECIFIED> EXCRETION FEW FECES EYES SQUINTED PERI-ORBITAL SKIN & PELAGE PALE ENTIRE BODY NORMAL NO REMARKABLE OBSERVATIONS DOSE: 2 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 1 , 8 , 15, 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 582- 583 498, 505, 512, 519, 526, 533, 540, 547 , 554, 561, 568, 575 582-583 491, 498 469-470, 477, 498, 547, 575, 582-583 561, 568, 575, 582-583 484, 491 498, 505, 512, 519, 526, 533 , 540, 547, 554, 561 , 568 575, 582 583 1 , 8 , 15 113, 120 204, 211 295, 302 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 660 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 119 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M3 (CONTINUED FROM PREVIOUS PAGE) C92680 M 84 NORMAL NO REMARKABLE OBSERVATIONS WART LIKE LESION(S) HEAD-MAXILLARY-LEFT WEEK 76 C92681 M 87 APPEARANCE THIN BEHAVIOR HYPOACTIVE <UNSPECIFIED> NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) TAIL-PROXIMAL WEEK 62 DOSE: 2 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463 526, 533, 540, 547 596, 603, 606 606 1 , 8 , 15 113, 120 204, 211 295, 302 386, 393 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 400, 407 , 414, 421 428, 435, 442, 449C, 456C, 463C, 470C, 477C, 484C, 491C, 498C 505C, 512C, 519C, 526C, 533C, 540C, 547C, 554C, 561C, 568C, 575C 582C, 589C, 596C, 603C, 606C 661 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 120 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M3 DOSE: 2 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92682 T 105 SKIN & PELAGE SORE/SCAB PAW-HIND-RIGHT NORMAL NO REMARKABLE OBSERVATIONS C92683 T 105 APPEARANCE SWOLLEN LIMB-HIND-LEFT NORMAL NO REMARKABLE OBSERVATIONS 722, 729, 731 1 , 8 ,r 15, 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582, 589, 596, 603, 610, 617 , 624, 631, 638, 645 , 652 659, 666, 673, 680, 687, 694, 701, 708 , 715 715, 722, 729, 733 1 , 8 ,r 15, 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582, 589, 596, 603, 610, 617 , 624, 631, 638, 645 , 652 659, 666, 673, 680, 687, 694, 701, 708 662 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 121 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M3 DOSE: 2 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92684 2 C92685 M 14 NORMAL NO REMARKABLE OBSERVATIONS 80 APPEARANCE MALOCCLUSION THIN BEHAVIOR HYPOACTIVE <UNSPECIFIED> RECUMBENT LATERAL DISCHARGE GENITAL RED IN COLOR EXCRETION FEW FECES EYES RED DISCHARGE EYES SQUINTED PERI-ORBITAL-RIGHT NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92-93 71, 78, 232, 239 547, 554 554 554 113 547, 554 554 554 1, 8, 15 134, 141 225, 246 330, 337 421, 428 22, 29, 36, 43,r 50, 148, 155 , 162, 169, 253, 260 , 267, 274, 344, 351 , 358, 365, 435, 442 , 449, 456, 57, 64, 85, 92, 99, 176, 183 , 190, 197, 281, 288 , 295, 302, 372, 379 , 386, 393, 463, 470 , 477, 484, 106, 204, 309, 400, 491, 120, 127 211, 218 316, 323 407, 414 498, 505 663 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 122 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M3 (CONTINUED FROM PREVIOUS PAGE) C92685 M 80 NORMAL NO REMARKABLE OBSERVATIONS SACRIFICE SACRIFICE APPROVED BY TOXICOLOGIST C92686 M 84 APPEARANCE MALOCCLUSION BEHAVIOR HYPOACTIVE <UNSPECIFIED> EXCRETION FEW FECES SKIN & PELAGE RED HAIR COAT VENTRAL-ABDOMINAL NORMAL NO REMARKABLE OBSERVATIONS DOSE: 2 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 512, 519, 526, 533, 540 554 554, 561, 568, 575, 582 582 575, 582 582 1 , 8 ,r 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407 , 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498 , 505, 512, 519, 526 , 533, 540, 547 664 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 123 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M3 DOSE: 2 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92687 M 72 APPEARANCE THIN EXCRETION FEW FECES EYES DILATED PUPIL EYES SKIN & PELAGE ROUGH HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS WART LIKE LESION(S) HEAD-MAXILLARY-LEFT WEEK 19 WEEK 57 477, 484, 491, 498, 502 469-470, 477, 484, 491, 498, 502 470, 502 470, 477, 484, 491 , 498, 502 1 , 8 ,r 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0, 183, 190 , 197, 204, 2 1 1 , 218 , 225, 232, 239, 246, 253 260, 267, 274, 281 , 288, 295, 302, 309 , 316, 323, 330, 337, 344 351, 358, 365, 372 , 379, 386 127, 134, 141, 148 , 155, 162, 169, 176 393, 400, 407, 414 , 421, 428, 435, 442 , 449, 456, 463, 469-470 477, 484, 491, 498 , 502 665 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 124 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M3 DOSE: 2 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92688 M 97 APPEARANCE SWOLLEN LIMB-HIND-LEFT PAW-HIND-LEFT THIN BEHAVIOR HYPOACTIVE <UNSPECIFIED> EXCRETION FEW FECES NORMAL NO REMARKABLE OBSERVATIONS D 56 APPEARANCE MALOCCLUSION NORMAL NO REMARKABLE OBSERVATIONS 498, 498, 589, 677 652, 505, 505, 596, 659, 512, 519 512, 519, 603,. 610, 666,. 673, 526, 617, 677 533, 624, 540, 631, 547 , 554, 638 , 645, 561, 652, 568, 659, 575 , 582 666 , 673 677 677 1 , 8 ,r 15, 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491 386, 390 1 , 8 ,r 15, 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 666 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 125 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M3 DOSE: 2 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92690 T 105 SKIN & PELAGE SORE/SCAB PAW-HIND-LEFT NORMAL NO REMARKABLE OBSERVATIONS 645, 652, 659,. 666, 673, 680, 687, 694 , 701, 708, 715, 722 , 729 733 1 , 8 ,r 15, 2 2 , 29, 36 , 43,r 50, 113, 1 2 0, 127,. 134, 141, 148, 204, 2 1 1 , 218, 225, 232, 239, 295, 302, 309,. 316, 323, 330, 386, 393, 400, 407, 414, 421, 477, 484, 491, 498, 505, 512, 568, 575, 582, 589, 596, 603, 57, 64, 71, 78, 85, 155, 162 , 169, 176, 246, 253 , 260, 267, 337, 344 , 351, 358, 428, 435 , 442, 449, 519, 526 , 533, 540, 610, 617 , 624, 631, 92, 99, 106 183, 190 , 197 274, 281 , 288 365, 372 , 379 456, 463 , 470 547, 554 , 561 638 667 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT M3 421 61 TO 428 62 MASS(ES) EAR-LEFT WEEK 48 435 63 TO 442 64 MASS(ES) EAR-LEFT WEEK 48 449 65 TO 484 70 MASS(ES) EAR-LEFT WEEK 48 491 71 MASS(ES) EAR-LEFT WEEK 48 498 72 TO 512 74 MASS(ES) EAR-LEFT WEEK 48 519 75 TO 610 CO CO MASS(ES) EAR-LEFT WEEK 48 617 89 MASS(ES) TO 708 102 EAR-LEFT WEEK 48 715 103 MASS(ES) TO 715 103 EAR-LEFT WEEK 48 10 X 10MM 15 X 10MM 15 X 15MM 25 X 20MM 10 X 10MM 15 X 15MM 20 X 20MM 30 X 30MM 668 Covance 6329-183 3M T-6295 PAGE: 126 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS CATEGORY KEYWORD WK QUALIFIER FREE-TEXT COMMENT 103 MASS(ES) 103 VENTRAL-CERVICAL-LEFT WEEK 103 76 MASS(ES) HEAD-MAXILLARY-RIGHT WEEK 76 77 MASS(ES) 79 HEAD-MAXILLARY-RIGHT WEEK 76 101 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 101 102 MASS(ES) VENTRAL-THORACIC-RIGHT WEEK 101 103 MASS(ES) 105 VENTRAL-THORACIC-RIGHT WEEK 101 64 MASS(ES) 66 VENTRAL-ABDOMINAL-LEFT WEEK 62 88 MASS(ES) VENTRAL-ABDOMINAL WEEK 88 25 X 45MM 15 X 15MM 20 X 15MM 40 X 40MM 40 X 50MM 60 X 60MM 10 X 10MM 10 X 10MM 669 Covance 6329-183 3M T-6295 PAGE: 127 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 89 MASS(ES) VENTRAL-ABDOMINAL WEEK 88 85 MASS(ES) 86 TAIL-MID WEEK 85 87 MASS(ES) 91 TAIL-MID WEEK 85 92 MASS(ES) 93 TAIL-MID WEEK 85 94 MASS(ES) 94 TAIL-MID WEEK 85 52 MASS(ES) 53 DORSAL WEEK 49 54 MASS(ES) 59 DORSAL WEEK 49 60 MASS(ES) DORSAL WEEK 49 20 X 20MM 10 X 10MM 20 X 15 20 X 20MM 20 X 25MM 10 X 10MM 15 X 15MM 30 X 30MM 670 Covance 6329-183 3M T-6295 PAGE: 128 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 61 MASS(ES) 70 DORSAL WEEK 49 71 MASS(ES) 80 DORSAL WEEK 49 81 MASS(ES) DORSAL WEEK 49 82 MASS(ES) 84 DORSAL WEEK 49 93 MASS(ES) 10 DORSAL WEEK 93 89 MASS(ES) 90 SHOULDER-LEFT WEEK 89 91 MASS(ES) SHOULDER-LEFT WEEK 89 92 MASS(ES) SHOULDER-LEFT WEEK 89 20 X 20MM 30 X 30MM 30 X 20MM 10 X 15MM 10 X 10MM 25 X 30MM 35 X 40MM 50 X 60MM 671 Covance 6329-183 3M T-6295 PAGE: 129 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 93 MASS(ES) SHOULDER-LEFT WEEK 89 94 MASS(ES) 95 SHOULDER-LEFT WEEK 89 69 MASS(ES) 72 VENTRAL-ABDOMINAL-RIGHT WEEK 69 73 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 69 74 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 69 75 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 69 76 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 69 76 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 69 70 X 70MM 70 X 80MM 30 X 30MM 30 X 30MM 30 X 30MM 30 X 30MM 30 X 30MM 30 X 30MM 672 Covance 6329-183 3M T-6295 PAGE: 130 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 76 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 69 77 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 69 78 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 69 79 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 69 79 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 69 79 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 69 29 MASS(ES) 31 VENTRAL-ABDOMINAL WEEK 29 82 MASS(ES) 83 AXILLARY REGION-LEFT WEEK 81 30 X 30MM 30 X 30MM 30 X 30MM 30 X 30MM 30 X 30MM 30 X 30MM 10 X 10MM 15 X 20MM 673 Covance 6329-183 3M T-6295 PAGE: 131 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 84 MASS(ES) 86 AXILLARY REGION-LEFT WEEK 81 87 MASS(ES) 88 AXILLARY REGION-LEFT WEEK 81 89 MASS(ES) 90 AXILLARY REGION-LEFT WEEK 81 91 MASS(ES) AXILLARY REGION-LEFT WEEK 81 92 MASS(ES) AXILLARY REGION-LEFT WEEK 81 93 MASS(ES) 95 AXILLARY REGION-LEFT WEEK 81 56 MASS(ES) 57 VENTRAL-ABDOMINAL-RIGHT WEEK 56 92 MASS(ES) 94 DORSAL WEEK 90 20 X 25MM 30 X 40MM 40 X 40MM 40 X 50MM 50 X 50MM 50 X 60MM 10 X 10MM 10 X 10MM 674 Covance 6329-183 3M T-6295 PAGE: 132 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C92675 C92681 M3 659 95 MASS(ES) TO 712 102 DORSAL WEEK 90 M3 449 65 TO 606 87 MASS(ES) TAIL-PROXIMAL WEEK 62 15 X 15MM 20 X 10MM Covance 6329-183 3M T-6295 PAGE: 133 675 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 134 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M4 DOSE: 5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92691 T 105 APPEARANCE MALOCCLUSION EYES DISCOLORED/LIGHT RED EYE-RIGHT NORMAL NO REMARKABLE OBSERVATIONS C92692 D 53 APPEARANCE MALOCCLUSION THIN EXCRETION FEW FECES NORMAL NO REMARKABLE OBSERVATIONS 589, 659, 666, 673, 708, 715 666617, 624, 631,. 638, 645, 652, 659, , 673, 680, 687, 694 , 701 708, 715, 722,. 729, 731 1 22, 8,r 15, , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 120113, , 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 211204, , 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582,. 596, 603, 610 148, 155, 309,. 316, 323, 330, 337, 344 , 351, 358, 365 358, 365 358, 365 1 22, 8,r 15, , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 120113, , 127,. 134, 141, 162, 169, 176 , 183, 190, 197, 204 , 211 218, 225, 232, 239, 246, 253, 260, 267 , 274, 281, 288, 295 , 302 676 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 135 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M4 DOSE: 5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92693 T 105 EYES RED DISCHARGE EYE-LEFT EYE-RIGHT SKIN & PELAGE BLACK SKIN DORSAL SORE/SCAB DORSAL NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) DORSAL WEEK 98 VENTRAL-ABDOMINAL WEEK 72 WART LIKE LESION(S) VENTRAL-ABDOMINAL WEEK 40 302, 400, 596 533 687, 694, 701, 708, 715, 722 729, 733 680, 687, 694, 701, 708, 715 722, 729 , 733 1 , 8 , 15, 2 2 , 29, 36 , 43 , 50 113, 1 2 0 , 127,. 134, 141, 148 204, 2 1 1 , 218, 225, 232, 239 323, 330, 337,. 344, 351, 358 421, 428, 435,. 442, 449, 456 526, 540, 547,. 554, 561, 568 631, 638, 645,. 652, 659, 666 57, 64, 71, 78, 85, 92, 99, 106 155, 162 , 169, 176, 183, 190 , 197 246, 253 , 260, 267, 295, 309 , 316 365, 372 , 379, 386, 393, 407 , 414 463, 470 , 477, 484, 491, 512 , 519 575, 582 , 589, 603, 610, 617 , 624 673 680C, 687C', 694C, 701C, 708C 715C, 722C, 729C, 733C 498C, 505C 274, 281, 288 677 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 136 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M4 DOSE: 5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92694 T 105 APPEARANCE MALOCCLUSION EYES RED DISCHARGE EYE-RIGHT SKIN & PELAGE SORE/SCAB MOUTH PAW-HIND-RIGHT NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) MOUTH WEEK 64 VENTRAL-ABDOMINAL-RIGHT WEEK 95 463, 526, 554, 561, 568, 575, 582, 589, 659, 708, 715 652 463, 470, 477, 603, 610, 617 715, 722, 729, 731 1 , 8 ,r 15, 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 484, 491, 498, 505 , 512 519, 533, 540, 547, 596, 624, 631, 638 , 645, 694, 701 442C, 449C, 456C 659C, 666C, 673C, 680C, 687C 678 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 137 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M4 DOSE: 5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92695 T 105 NORMAL NO REMARKABLE OBSERVATIONS C92696 T 105 SKIN & PELAGE SORE/SCAB PAW-HIND-RIGHT NORMAL NO REMARKABLE OBSERVATIONS C92697 M 67 APPEARANCE THIN BEHAVIOR RECUMBENT LATERAL 1, 8 15, 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582, 589, 596, 603, 610, 617 , 624, 631, 638, 645 , 652 659, 666, 673, 680, 687, 694, 701, 708 , 715, 722, 729, 731 722, 729, 731 1 , 8 ,r 15, 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582, 589, 596, 603, 610, 617 , 624, 631, 638, 645 , 652 659, 666, 673, 680, 687, 694, 701, 708 , 715 463, 465 465 679 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 138 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M4 (CONTINUED FROM PREVIOUS PAGE) C92697 M 67 EXCRETION NO FECES EYES RED DISCHARGE EYES SKIN & PELAGE SORE/SCAB DORSAL-THORACIC NORMAL NO REMARKABLE OBSERVATIONS C92698 T MASS(ES) DORSAL WEEK 58 105 EXCRETION FEW FECES LIQUID FECES EYES PALE EYES DOSE: 5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 465 465 414, 421, 428, 435 , 442, 449, 456, 463 , 465 1 , 8 ,r 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393 400C, 407C, 414C, 421C, 428C, 435C, 442C, 449C, 456C, 463C, 465C 729, 732 729, 732 729, 732 680 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 139 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M4 (CONTINUED FROM PREVIOUS PAGE) C92698 T 105 SKIN & PELAGE BROWN HAIR COAT PERINEAL AREA SORE/SCAB AXILLARY REGION-LEFT OTHER NORMAL NO REMARKABLE OBSERVATIONS WART LIKE LESION(S) AXILLARY REGION-LEFT WEEK 82 C92699 T 105 APPEARANCE MALOCCLUSION NORMAL NO REMARKABLE OBSERVATIONS DOSE: 5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 729, 732 687, 694, 701, 708, 715, 722, 729, 732 72 9C, 732C 1 , 8 ,r 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407 , 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498 , 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582, 589, 596, 603, 610, 617, 624, 631, 638, 645, 652 659, 666, 673, 680, 687, 694, 701, 708, 715, 722, 729, 732 99, 106, 113, 1 2 0 , 127, 134, 141, 148, 155, 162, 169, 176, 183 190, 197, 204, 2 1 1 , 218, 225, 232, 239, 246, 253, 260, 267, 274 281, 288, 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365 372, 379, 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456 463, 470, 477, 484, 491, 498, 505, 512, 519, 526, 533, 540, 547 554, 561, 568, 575, 582, 589, 596, 603, 610, 617, 624, 631, 638 645, 652, 659, 666, 673, 680, 687, 694, 701, 708, 715, 722, 729 731 1 , 8 , 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92 681 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 140 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M4 DOSE: 5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92700 T 105 APPEARANCE MALOCCLUSION SWOLLEN VENTRAL-ABDOMINAL BEHAVIOR HYPOACTIVE <UNSPECIFIED> EXCRETION NONFORMED FECES SKIN & PELAGE ROUGH HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) AXILLARY REGION-LEFT WEEK 98 VENTRAL-ABDOMINAL-LEFT WEEK 58 554, 561, 568, 575 323, 330, 414, 421, 428, 435, 442, 449, 470, 477, 484, 491, 498 505, 512, 519, 526, 533, 540, 547, 554, 561, 568, 575, 582, 589 596, 603, 610, 617, 624, 631, 638, 645, 652, 659, 666, 673, 680 687, 694, 701, 708, 715, 722, 729, 733 722 288 78, 85, 92, 99, 106, 1 2 0 , 127, 134 , 141, 148, 155, 162, 169, 176 183, 190 , 197 , 204, 2 1 1 , 218, 225, 232, 239, 246, 253, 260, 267 288, 295 , 302 , 309, 316, 323, 330, 337, 344, 351, 358, 365, 372 379, 386 , 393 , 400, 407, 414, 421, 428, 435, 442, 449, 456, 463 470, 477 , 484 , 491, 498, 505, 512, 519, 526, 533, 540, 547, 554 561, 568 , 575 , 582, 589, 596, 603, 610, 617, 624, 631, 638, 645 652, 659 , 666 , 673, 680, 687 1 , 8 , 15 , 2 2 , 29, 36 , 43,r 50, 57, 64, 71 , 113 , 274 , 281 680C, 687C, 694C, 701C, 708C, 715C, 722C, 729C, 733C 400C 682 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 141 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M4 DOSE: 5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92701 M 53 APPEARANCE HUNCHED THIN BEHAVIOR COLD TO TOUCH ENTIRE BODY EXCRETION FEW FECES EYES RED DISCHARGE EYE-RIGHT SKIN & PELAGE PALE ENTIRE BODY SORE/SCAB LIMB-FRONT-RIGHT NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) LIMB-FRONT-RIGHT WEEK 47 365 365 365 365 365 365 351, 358, 365 1 , 8 , 15, 2 2 , 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127,. 134, 141, 148, 155, 162, 169, 176, 183,. 190, 197 204, 2 1 1 , 218,. 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316 32 3C, 330C , 337C, 344C, 351C, 358C , 365C 683 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 142 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M4 DOSE: 5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92702 T 105 APPEARANCE HEAD TILT LEFT NORMAL NO REMARKABLE OBSERVATIONS C92703 D 61 NORMAL NO REMARKABLE OBSERVATIONS C92704 T 105 NORMAL NO REMARKABLE OBSERVATIONS 722, 729, 732 1 , 8 ,r 15, 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582, 589, 596, 603, 610, 617 , 624, 631, 638, 645 , 652 659, 666, 673, 680, 687, 694, 701, 708 , 715 1 , 8 ,r 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190, 197 204, 2 1 1 , 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281, 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372, 379 386, 393, 400, 407 , 414, 421, 423 1 , 8 ,r 15, 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582, 589, 596, 603, 610, 617 684 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 143 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M4 (CONTINUED FROM PREVIOUS PAGE) C92704 T 105 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 90 C92705 T 105 EYES DISCOLORED/LIGHT RED EYE-RIGHT NORMAL NO REMARKABLE OBSERVATIONS C92706 M 98 APPEARANCE LIMITED USE LIMBS-HIND BEHAVIOR HYPOACTIVE <UNSPECIFIED> EYES RED DISCHARGE EYES DOSE: 5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 624, 631, 638, 645, 652, 659C, 666C, 673C, 680C, 687C, 694C 701C, 708C, 715C, 722C, 729C, 732C 715, 722, 729, 731 1 , 8 ,r 15, 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582, 589, 596, 603, 610, 617 , 624, 631, 638, 645 , 652 659, 666, 673, 680, 687, 694, 701, 708 680 680 680 685 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 144 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M4 (CONTINUED FROM PREVIOUS PAGE) C92706 M 98 SKIN & PELAGE SORE/SCAB VENTRAL-ABDOMINAL-LEFT VENTRAL-ABDOMINAL-RIGHT NORMAL NO REMARKABLE OBSERVATIONS C92707 M MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 82 VENTRAL-ABDOMINAL-RIGHT WEEK 82 90 APPEARANCE THIN BEHAVIOR HYPOACTIVE <UNSPECIFIED> DISCHARGE NASAL RED IN COLOR DOSE: 5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 575, 582 596, 603, 610 1 , 8 ,r 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407 , 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498 , 505, 512, 519, 526 , 533, 540, 547, 554 , 561 617, 624, 631, 638 , 645, 652, 659, 666 , 673 568C 568C, 575C, 582C, 589C, 596C 617, 624, 627 624, 627 624, 627 686 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 145 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M4 (CONTINUED FROM PREVIOUS PAGE) C92707 M 90 EXCRETION FEW FECES EYES SQUINTED PERI-ORBITAL SKIN & PELAGE ROUGH HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS T 105 APPEARANCE MALOCCLUSION EYES CLEAR DISCHARGE EYE-RIGHT DOSE: 5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 624 624, 627 617, 624, 627 1 , 8 , 15 , 2 2 , 29, 36 , 43 , 50 , 57, 64, 71, 78, 85, 92, 99, 106 113, 120 , 127, 134, 141, 148 , 155, 162 , 169, 176, 183, 190 , 197 204, 211 , 218, 225, 232, 239 , 246, 253 , 260, 267, 274, 281 , 288 295, 302 , 309, 316, 323, 330 , 337, 344 , 351, 358, 365, 372 , 379 386, 393 , 400, 407, 414, 421 , 428, 435 , 442, 449, 456, 463 , 470 477, 484 , 491, 498, 505, 512 , 519, 526 , 533, 540, 547, 554 , 561 568, 575 , 582, 589, 596, 603 , 610 36, 43, 50, 57 , 64, 141, 148 , 155, 162, 232, 239 , 246, 253, 323, 330 , 337, 344, 414, 421 , 428, 435, 505, 512 , 519, 526, 596, 603 , 610, 617, 687, 694 , 701, 708, 71, 78, 85, 92 , 99 , 106, 169, 176 , 183, 190 , 197, 260, 267 , 274, 281 , 288, 351, 358 , 365, 372 , 379, 442, 449 , 456, 463 , 470, 533, 540 , 547, 554 , 561, 624, 631 , 638, 645 , 652, 715, 722 , 729, 731 113, 204, 295, 386, 477, 568, 659, 1 2 0 , 127 , 134 2 1 1 , 218 , 225 302, 309 , 316 393, 400 , 407 484, 491 , 498 575, 582 , 589 666, 673 , 680 540 687 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 146 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M4 (CONTINUED FROM PREVIOUS PAGE) C92708 T 105 EYES RED DISCHARGE EYE-RIGHT NORMAL NO REMARKABLE OBSERVATIONS C92709 D 91 APPEARANCE MALOCCLUSION THIN DISCHARGE NASAL RED IN COLOR SKIN & PELAGE PALE ENTIRE BODY NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) PAW-HIND-RIGHT WEEK 77 DOSE: 5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 456 1, 8 , 15, 22, 29 274, 281, 288, 330, 337, 344, 442, 463, 484, 491, 498, 505, 512 519, 575, 582, 589 617, 624, 631, 633 596 631, 633 1 , 8 , 15 113, 120 204, 211 316, 323 428, 435 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 218, 225 , 232, 239, 246, 253 , 260, 267, 295, 302 , 309 351, 358 , 365, 372, 379, 386 , 393, 400, 407, 414 , 421 449, 456 , 470, 477, 526, 554 , 561, 568, 603, 610 533, 540, 547 688 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 147 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M4 DOSE: 5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92710 D 70 SKIN & PELAGE SORE/SCAB LATERAL-LEFT NORMAL NO REMARKABLE OBSERVATIONS C92711 D MASS(ES) DORSAL WEEK 54 92 DISCHARGE GENITAL RED IN COLOR NORMAL NO REMARKABLE OBSERVATIONS WART LIKE LESION(S) DORSAL-CERVICAL-LEFT WEEK 90 442 1 22, 8,r 15, , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 120113, , 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 211204, , 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 449 , 456 463, 470, 477, 484 , 486 372, 379, 386, 393 , 400, 407, 414, 421 , 428C, 435C 456 111,3,8,r11250,, 22, 127 29, 36 , 43,r 50, 134, 141, 148, 204, 211, 218 225, 232, 239, 295, 302, 309 316, 323, 330, 386, 393, 400 407, 414, 421, 484, 491, 498 505, 512, 519, 575, 582, 589 596, 603, 610, 57, 64, 71, 78, 85, 155, 162 , 169, 176, 246, 253 , 260, 267, 337, 344 , 351, 358, 428, 435 , 442, 449, 526, 533 , 540, 547, 617, 631 , 638, 640 92, 99, 106 183, 190 , 197 274, 281 , 288 365, 372 , 379 463, 470 , 477 554, 561 , 568 624 689 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 148 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M4 DOSE: 5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92712 M 87 APPEARANCE THIN BEHAVIOR HYPOACTIVE <UNSPECIFIED> EXCRETION FEW FECES EYES SQUINTED PERI-ORBITAL NORMAL NO REMARKABLE OBSERVATIONS C92713 2 14 NORMAL NO REMARKABLE OBSERVATIONS 596, 603 603 581-582, 589 596, 603 603 1 , 8 , 15 113, 120 204, 211 295, 302 386, 393 477, 484 568, 575 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 127, 134 , 141, 148, 155, 162 , 169, 176, 183 , 190 , 197 218, 225 , 232, 239, 246, 253 , 260, 267, 274 , 281 , 288 309, 316 , 323, 330, 337, 344 , 351, 358, 365 , 372 , 379 400, 407 , 414, 421, 428, 435 , 442, 449, 456 , 463 , 470 491, 498 , 505, 512, 519, 526 , 533, 540, 547 , 554 , 561 1 , 8 , 15 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92- 93 690 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 149 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M4 DOSE: 5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92714 2 C92715 1 C92716 T 14 NORMAL NO REMARKABLE OBSERVATIONS 4 NORMAL NO REMARKABLE OBSERVATIONS 105 APPEARANCE THIN EXCRETION FEW FECES NONFORMED FECES EYES DISCOLORED/LIGHT RED EYES SKIN & PELAGE BROWN HAIR COAT PERINEAL AREA ROUGH HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS 1 , 8 ,r 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92- 93 1 , 8 ,r 15, 22 411, 414, 421, 428 , 435, 526, 533, 540 , 547, 554, 561 , 568, 575 582, 589, 596, 603 , 610, 617, 624, 631 , 638, 645, 652 , 659, 666 673, 680, 687,. 694 , 701, 708, 715, 722 , 729, 732 411, 414 519, 729, 732 386, 393, 400, 407 , 411, 414, 421, 428 , 435, 442, 449 , 456, 463 470, 477, 484, 491 , 498, 505, 512, 519 , 526, 533, 540 , 547, 554 561, 568, 575, 582 , 589, 596, 603, 610 , 617, 624, 631 , 638, 645 652, 659, 666, 673 , 680, 687, 694, 701 , 708, 715, 722 , 729, 732 414 414, 421 1 , 8 ,r 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 691 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 150 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M4 (CONTINUED FROM PREVIOUS PAGE) C92716 T 105 MASS(ES) MOUTH WEEK 83 WEEK 95 C92717 T 105 SKIN & PELAGE SORE/SCAB DORSAL NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) DORSAL WEEK 98 VENTRAL-THORACIC-LEFT WEEK 86 DOSE: 5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 575C, 582C 659, 666 729, 733 1 , 8 ,. 15, 2 2 , 29, 36, 43 , 50, 57, 64, 71, 78 , 85, 92, 99, 106 113, 1 2 0, 127 , 134, 141, 148, 155, 162, 169, 176, 183, 190 , 197 204, 2 1 1 , 218 , 225, 232, 239, 246, 253, 260, 267, 274, 281 , 288 295, 302, 309 , 316, 323, 330, 337, 344, 351, 358, 365, 372 , 379 386, 393, 400 , 407, 414, 421, 428, 435, 442, 449, 456, 463 , 470 477, 484, 491 , 498, 505, 512, 519, 526, 533, 540, 547, 554 , 561 568, 575, 582 , 589 680C, 687C , 6 94C, 701C, 708C, 715C , 722C, 72 9, 733 596C, 603C , 6 10C, 617C, 624C, 631C , 638C, 64 5C, 652C, 659C , 666 673C, 680C , 6 87C, 694C, 701C, 708C , 715C, 722C, 729C, 733C 692 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 151 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M4 DOSE: 5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92718 1 C92719 2 D 4 NORMAL NO REMARKABLE OBSERVATIONS 14 NORMAL NO REMARKABLE OBSERVATIONS 83 SKIN & PELAGE SORE/SCAB VENTRAL-ABDOMINAL-RIGHT NORMAL NO REMARKABLE OBSERVATIONS C92721 M MASS(ES) VENTRAL-ABDOMINAL WEEK 76 80 APPEARANCE MALOCCLUSION MISSING TEETH 1, 8 , 15, 22 1 , 8 , 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92- 93 575- 576 1 , 8 , 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0, 127,. 134, 141, 148, 155,. 162, 169, 176, 183 , 190 , 197 204, 2 1 1 , 218,. 225, 232, 239, 246,. 253, 260, 267, 274 , 281 , 288 295, 302, 309,. 316, 323, 330, 337,. 344, 351, 358, 365 , 372 , 379 386, 393, 400,. 407, 414, 421, 428,. 435, 442, 449, 456 , 463 , 470 477, 484, 491, 498, 505, 512, 519 526C, 533C, 540C, 547C, 554C, 561C, 568C, 575-576C 29, 36, 43 , 50,r 57, 64, 71, 78, 85,r 92, 99, 106, 113, 1 2 0, 127 134, 141, 148, 155, 162, 169 , 176, 183, 190, 197, 204, 2 1 1 , 218 225, 232, 239, 246, 253, 260 , 267, 274, 281, 288, 295, 302, 309 316, 323, 330, 337, 344, 351 , 358, 365, 372, 379,. 386, 393, 400 407, 414, 421, 428, 435, 442 , 449, 456, 463, 470, 477, 484, 491 498, 505, 512, 519, 526, 533 , 540, 547, 554- 555, 557 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281,. 288, 295, 302 309, 316, 323, 330, 337, 344 , 351, 358, 365, 372, 379, 386, 393 693 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 152 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER (CONTINUED FROM PREVIOUS PAGE) C92721 M 80 APPEARANCE MISSING TEETH GROUP: M4 BEHAVIOR HYPOACTIVE <UNSPECIFIED> SENSITIVE TO TOUCH EXCRETION FEW FECES NONFORMED FECES EYES SQUINTED PERI-ORBITAL-RIGHT SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE NORMAL NO REMARKABLE OBSERVATIONS DOSE: 5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470, 477, 484 491, 498, 505, 512, 519, 526, 533, 540, 547, 554-555, 557 557 557 555, 557 557 557 557 1, 8 , 15, 22 694 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 153 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M4 DOSE: 5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92722 2 C92723 M 14 NORMAL NO REMARKABLE OBSERVATIONS 79 BEHAVIOR HYPOACTIVE <UNSPECIFIED> EXCRETION FEW FECES EYES RED DISCHARGE EYES SQUINTED PERI-ORBITAL-RIGHT SKIN & PELAGE RED HAIR COAT VENTRAL-ABDOMINAL NORMAL NO REMARKABLE OBSERVATIONS 1, 8 , 15, 22, 29, 36, 43, 50 57, 64, 71, 78 85 92-93 552 551-552 552 552 552 1 , 8 ,r 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0, 127 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400 407 , 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491 498 , 505, 512, 519, 526 , 533, 540, 547 695 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 154 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M4 DOSE: 5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92724 T 105 SKIN & PELAGE SORE/SCAB DORSAL VENTRAL-ABDOMINAL NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) DORSAL WEEK 74 INGUINAL REGION-RIGHT WEEK 86 VENTRAL-ABDOMINAL WEEK 73 WART LIKE LESION(S) DORSAL WEEK 96 596, 603, 610, 617, 624, 631, 638, 645, 652, 659 610, 617, 624, 631, 638, 645, 652, 659, 666, 673, 680, 687, 694 701, 708, 715, 722, 729, 731 1 , 8 ,r 15, 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498 512, 519, 526, 533, 540,, 547C, 554C , 561C, 568C, 575C, 582C 58 9C, 596C, 603C, 610C, 617C, 624C 596C, 603C, 610C, 617C, 624C 505, 512, 519, 526, 533,, 540C, 547C , 554C, 561C, 568C, 575C 582C,. 589C, 596C, 603C, 610C, 617C, 624C, 631C, 638C, 645C, 652C 659C, 666C, 673C, 680C, 687C, 694C, 701C, 708C, 715C, 722C, 72 9C 731C 666, 673, 680, 687, 694, 701, 708, 715, 722, 729, 731 696 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 155 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M4 DOSE: 5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92725 D 52 BEHAVIOR HYPOACTIVE <UNSPECIFIED> SENSITIVE TO TOUCH EXCRETION FEW FECES EYES RED DISCHARGE EYES RESPIRATION AUDIBLE LABORED SKIN & PELAGE RED HAIR COAT HEAD-MAXILLARY-LEFT HEAD-MAXILLARY-RIGHT NORMAL NO REMARKABLE OBSERVATIONS 363 363 363 363 363 363 363 363 1 , 8 ,r 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0, 127, 134 , 141, 148, 155, 162 , 169, 176, 183,. 190, 197 204, 2 1 1 , 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281, 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358 697 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 156 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M4 DOSE: 5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92726 T 105 APPEARANCE SWOLLEN PAW-HIND-RIGHT EXCRETION FEW FECES SKIN & PELAGE SORE/SCAB PAW-HIND-LEFT NORMAL NO REMARKABLE OBSERVATIONS C92727 D 90 DISCHARGE GENITAL RED IN COLOR SKIN & PELAGE SORE/SCAB PAW-HIND-LEFT NORMAL NO REMARKABLE OBSERVATIONS 659, 666, 673, 680 624 624 1 , 8 ,r 15, 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0, 127 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582 589, 596, 603, 610, 617 , 631, 638, 645, 652 , 687 694, 701, 708 715, 722, 729, 733 64 540, 547, 554, 561, 568, 575, 582, 589, 596, 603, 610, 617, 624 630 1 , 8 15, 22, 29, 36, 43, 50, 57, 71, 78, 85, 92 99, 106, 113 698 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 157 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M4 (CONTINUED FROM PREVIOUS PAGE) C92727 D 90 NORMAL NO REMARKABLE OBSERVATIONS C92728 2 C92729 M NORMAL NO REMARKABLE OBSERVATIONS 79 APPEARANCE THIN EXCRETION FEW FECES NO FECES EYES RED DISCHARGE EYE-RIGHT EYES SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE NORMAL NO REMARKABLE OBSERVATIONS DOSE: 5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 1 2 0, 127, 2 1 1 , 218, 302, 309, 393, 400, 484, 491, 134, 225, 316, 407, 498, 141 , 148, 232 , 239, 323 , 330, 414 , 421, 505 , 512, 155, 246, 337, 428, 519, 162, 253, 344, 435, 526, 169 , 176, 260 , 267, 351 , 358, 442 , 449, 533 183, 274, 365, 456, 190 , 197, 281 , 288, 372 , 379, 463 , 470, 204 295 386 477 1 , 8 ,r 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92- 93 547, 549 540, 547 549 540, 547 549 505, 512 519, 526 , 533, 540, 547, 549 1 , 8 , 15 113, 120 204, 211 295, 302 386, 393 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 400, 407 , 414, 421, 428, 435 , 442, 449, 456, 463 , 470 699 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 158 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M4 (CONTINUED FROM PREVIOUS PAGE) C92729 M 79 NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) INGUINAL REGION-LEFT WEEK 77 C92730 T 105 BEHAVIOR ATAXIC <UNSPECIFIED> EXCRETION FEW FECES NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) HEAD-MAXILLARY-LEFT WEEK 105 DOSE: 5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 477, 484, 491, 498 533C, 540C, 547C, 549C 729, 732 715, 722 1 , 8 ,r 15, 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582, 589, 596, 603, 610, 617 , 624, 631, 638, 645 , 652 659, 666, 673, 680, 687, 694, 701, 708 729, 732 700 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 159 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M4 DOSE: 5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92731 1 C92732 D 4 NORMAL NO REMARKABLE OBSERVATIONS 83 NORMAL NO REMARKABLE OBSERVATIONS C92733 M 89 APPEARANCE THIN EXCRETION FEW FECES NO FECES SKIN & PELAGE RED HAIR COAT LATERAL-ABDOMINAL-RIGHT ROUGH HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS 1, 8 , 15, 22 1 , 8 , 15, 2 2 , 29, 36, 43 , 50, 113, 1 2 0, 127, 134 , 141, 148, 204, 2 1 1 , 218, 225 , 232, 239, 295, 302, 309, 316 , 323, 330, 386, 393, 400, 407 , 414, 421, 477, 484, 491, 498 , 505, 512, 568, 575, 578 57, 64, 71, 78, 85, 155, 162 , 169, 176, 246, 253 , 260, 267, 337, 344 , 351, 358, 428, 435 , 442, 449, 519, 526 , 533, 540, 92, 99, 106 183, 190 , 197 274, 281 , 288 365, 372 , 379 456, 463 , 470 547, 554 , 561 589- 590, 596, 603, 610, 614, 617 590, 596 614, 617 617 617 1 , 8 , 15 113, 120 204, 211 295, 302 386, 393 477, 484 568, 575 2 2 , 29, 36, 43,r 50, 127, 134 , 141, 148, 218, 225 , 232, 239, 309, 316 , 323, 330, 400, 407 , 414, 421, 491, 498 , 505, 512, 582 57, 64, 71, 78, 85, 155, 162 , 169, 176, 246, 253 , 260, 267, 337, 344 , 351, 358, 428, 435 , 442, 449, 519, 526 , 533, 540, 92, 99, 106 183, 190 , 197 274, 281 , 288 365, 372 , 379 456, 463 , 470 547, 554 , 561 701 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 160 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M4 DOSE: 5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92734 1 C92735 T 4 NORMAL NO REMARKABLE OBSERVATIONS 105 SKIN & PELAGE SORE/SCAB VENTRAL-ABDOMINAL NORMAL NO REMARKABLE OBSERVATIONS C92736 T MASS(ES) VENTRAL-ABDOMINAL WEEK 27 105 DISCHARGE CLEAR ORAL MILD NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22 197 111,3,8,r11250,, 22, 127 225, 232, 239 316, 323, 330 407, 414, 421 498, 505, 512 589, 596, 603 680, 687, 694 2119, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 134, 141, 148, 155, 162 , 169, 176, 204, , 218 246, 253, 260, 267, 274 , 281, 288, 295, 302 , 309 337, 344, 351, 358, 365 , 372, 379, 386, 393 , 400 428, 435, 442, 449, 456 , 463, 470, 477, 484 , 491 666519, 526, 533, 540, 547 , 554, 561, 568, 575 , 582 610, 617, 624, 631, 638 , 645, 652, 659, , 673 701, 708, 715, 722, 729 , 731 183C, 190C 15 112,08,,r12272,, 29, 134 211, 218, 225 302, 309, 316 393, 400, 407 484, 491, 498 6, 43 , 50,r 57, 64, 71, 78, 85,r 92, 99, 106, 113 141, 148, 155, 162, 169 , 176, 183, 190, 197, 204 232, 239, 246, 253, 260 , 267, 274, 281,. 288, 295 323, 330, 337, 344, 351 , 358, 365, 372, 379, 386 414, 421, 428, 435, 442 , 449, 456, 463,. 470, 477 505, 512, 519, 526, 533 , 540, 547, 554, 561, 568 702 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 161 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M4 (CONTINUED FROM PREVIOUS PAGE) C92736 T 105 NORMAL NO REMARKABLE OBSERVATIONS C92737 M 91 APPEARANCE CONVULSIONS BEHAVIOR HYPOACTIVE <UNSPECIFIED> EXCRETION FEW FECES NO FECES RESPIRATION IRREGULAR NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) DORSAL WEEK 78 DOSE: 5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 575, 582, 589, 596, 603, 610 , 617, 624 , 631, 638, 645, 652, 659 666, 673, 680, 687, 694, 701 , 708, 715 , 722, 729, 733 637 637 624, 631 637 637 1 , 8 ,r 15, 2 2 , 29, 36 , 43, 50 , 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127, 134, 141, 148 , 155, 162 , 169, 176, 183, 190, 197 204, 2 1 1 , 218, 225, 232, 239 , 246, 253 , 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330 , 337, 344 , 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421 , 428, 435 , 442, 449, 456, 463, 470 477, 484, 491, 498, 505, 512 , 519, 526 , 533 540, 547, 554C , 561C , 568C, 575C, 582C , 589C, 596C , 603C, 610C 617C, 624C, 631C, 637C 703 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 162 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M4 DOSE: 5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92738 D 92 NORMAL NO REMARKABLE OBSERVATIONS C92739 T 105 NORMAL NO REMARKABLE OBSERVATIONS C92740 T 105 APPEARANCE MALOCCLUSION SKIN & PELAGE SORE/SCAB VENTRAL-ABDOMINAL-RIGHT NORMAL NO REMARKABLE OBSERVATIONS 1 , 8 ,r 15, 2 2 , 29, 36 , 43,r 50, 113, 1 2 0 , 127,. 134, 141, 148, 204, 2 1 1 , 218, 225, 232, 239, 295, 302, 309,. 316, 323, 330, 386, 393, 400, 407, 414, 421, 477, 484, 491, 498, 505, 512, 568, 575, 582, 589, 596, 603, 57, 64, 71, 78, 85, 155, 162 , 169, 176, 246, 253 , 260, 267, 337, 344 , 351, 358, 428, 435 , 442, 449, 519, 526 , 533, 540, 610, 617 , 624, 631, 92, 99, 106 183, 190 , 197 274, 281 , 288 365, 372 , 379 456, 463 , 470 547, 554 , 561 638, 641 1 , 8 ,r 15, 2 2 , 29, 36, 43,r 50, 113, 1 2 0 , 127, 134 , 141, 148, 204, 2 1 1 , 218, 225 , 232, 239, 295, 302, 309, 316 , 323, 330, 386, 393, 400, 407 , 414, 421, 477, 484, 491, 498 , 505, 512, 568, 575, 582, 589 , 596, 603, 659, 666, 673, 680 , 687, 694, 57, 64, 71, 78, 85, 155, 162 , 169, 176, 246, 253 , 260, 267, 337, 344 , 351, 358, 428, 435 , 442, 449, 519, 526 , 533, 540, 610, 617 , 624, 631, 701, 708 , 715, 722, 92, 99, 106 183, 190 , 197 274, 281 , 288 365, 372 , 379 456, 463 , 470 547, 554 , 561 638, 645 , 652 729, 733 225, 232, 239, 246 , 253, 575, 582, 589 , 596, 603, 610, 617 , 624 631, 638, 645, 652 , 659 666, 673, 680, 687, 694 1 , 8 ,r 15, 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190, 197 204, 2 1 1 , 218, 260, 267, 274, 281, 288 , 295, 302, 309, 316, 323 330, 337, 344, 351, 358, 365, 372, 379 , 386, 393, 400, 407, 414 704 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 163 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M4 (CONTINUED FROM PREVIOUS PAGE) C92740 T 105 NORMAL NO REMARKABLE OBSERVATIONS C92741 M MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 93 91 APPEARANCE HUNCHED THIN BEHAVIOR HYPOACTIVE <UNSPECIFIED> RECUMBENT LATERAL COLD TO TOUCH ENTIRE BODY EXCRETION FEW FECES SKIN & PELAGE SORE/SCAB PAW-HIND-LEFT PAWS-HIND YELLOW HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS DOSE: 5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 421, 428, 435, 442, 449, 456, 463, 470, 477, 484, 491, 498, 505 512, 519, 526, 533, 540, 547, 554, 561, 568, 701, 708, 715, 722 729, 733 64 5C, 652C, 659C 633 617, 624, 631, 633 624, 631, 633 633 633 624 540, 547, 554, 561, 568 575, 582, 589, 596, 603, 610, 617, 624, 631, 633 617 1, 8 , 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 705 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 164 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M4 (CONTINUED FROM PREVIOUS PAGE) C92741 M 91 NORMAL NO REMARKABLE OBSERVATIONS T 105 SKIN & PELAGE SORE/SCAB PAW-HIND-RIGHT NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) DORSAL WEEK 51 WART LIKE LESION(S) DORSAL WEEK 66 DOSE: 5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 113, 204, 295, 386, 477, 12 0 , 127, 2 1 1 , 218, 302, 309, 393, 400, 484, 491, 134 , 141, 225 , 232, 316 , 323, 407 , 414, 498 , 505, 148, 239, 330, 421, 512, 155, 246, 337, 428, 519, 162, 253, 344, 435, 526, 169, 260, 351, 442, 533 176 , 183, 267 , 274, 358 , 365, 449 , 456, 190 , 197 281 , 288 372 , 379 463 , 470 610, 680, 687,. 694 , 701, 708, 715, 722, 729, 732 1 , 8 , 15, 2 2 , 29, 36, 43 , 50, 57, 64, 71 , 78, 85 , 92, 99, 106 113, 1 2 0 , 127, 134 , 141, 148, 155, 162, 169, 176 , 183, 190 , 197 204, 2 1 1 , 218, 225 , 232, 239, 246, 253, 260, 267 , 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344, 414, 421 , 428, 435 , 442 449 351C, 358C , 365C, 372C, 379C, 386C , 393C , 400C, 407C 456, 463 547, 554 638, 645 729, 732 470, 477, 484, 491, 498, 505, 512, 519, 526, 533, 540 561, 568, 575, 582, 589, 596, 603, 610, 617, 624, 631 652, 659, 666, 673, 680, 687, 694, 701, 708, 715, 722 706 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 165 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M4 DOSE: 5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92743 D 84 APPEARANCE OBES SWOLLEN VENTRAL-ABDOMINAL NORMAL NO REMARKABLE OBSERVATIONS C92744 1 C92745 M 4 NORMAL NO REMARKABLE OBSERVATIONS 90 APPEARANCE SWOLLEN VENTRAL-ABDOMINAL BEHAVIOR HYPOACTIVE <UNSPECIFIED> EXCRETION FEW FECES EYES PALE EYES 582, 587 456 1 , 8 ,r 15, 2 2 , 29, 36 , 43,r 50, 113, 1 2 0 , 127,. 134, 141, 148, 204, 2 1 1 , 218, 225, 232, 239, 295, 302, 309,. 316, 323, 330, 386, 393, 400, 407, 414, 421, 484, 491, 498, 505, 512, 519, 575 57, 64, 71, 78, 85, 155, 162 , 169, 176, 246, 253 , 260, 267, 337, 344 , 351, 358, 428, 435 , 442, 449, 526, 533 , 540, 547, 92, 99, 106 183, 190 , 197 274, 281 , 288 365, 372 , 379 463, 470 , 477 554, 561 , 568 1, 8 , 15, 22 456 624 596 596, 603 707 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 166 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M4 (CONTINUED FROM PREVIOUS PAGE) C92745 M 90 SKIN & PELAGE PALE ENTIRE BODY SORE/SCAB DORSAL PAWS-HIND VENTRAL-ABDOMINAL-LEFT VENTRAL-ABDOMINAL-RIGHT NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) DORSAL WEEK 74 INGUINAL REGION-RIGHT WEEK 84 VENTRAL-ABDOMINAL-LEFT WEEK 46 VENTRAL-ABDOMINAL-RIGHT WEEK 70 DOSE: 5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 610, 617, 624 554, 561, 568,. 575, 582, 589, 596, 603 , 610, 617, 624 596, 603, 610, 617, 624 344 561, 568 1 , 8 ,r 15, 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 351, 358, 365, 372, 379 , 386, 393, 400, 407 , 414 421, 428, 435,. 442, 449, 463, 470, 477 512C, 519C , 526C , 533C , 540C, 547C , 554C, 561C, 568C, 575C, 582C 582, 589, 596C, 603C, 610C, 617C, 624C 316C, 323C , 330C , 337C 484C, 491C , 498C , 505C , 512C, 519C , 526C, 533C, 54 0C, 54 7C, 554C 708 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 167 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M4 DOSE: 5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92746 M 79 BEHAVIOR HYPOACTIVE <UNSPECIFIED> COLD TO TOUCH ENTIRE BODY EXCRETION NO FECES NORMAL NO REMARKABLE OBSERVATIONS C92747 T 105 APPEARANCE SWOLLEN PAW-FRONT-RIGHT PAW-HIND-RIGHT EYES RED DISCHARGE EYE-RIGHT NORMAL NO REMARKABLE OBSERVATIONS 551 551 551 1 , 8 ,r 15, 2 2 , 29, 36, 43,r 50, 113, 1 2 0 , 127, 134 , 141, 148, 204, 2 1 1 , 218, 225 , 232, 239, 295, 302, 309, 316 , 323, 330, 386, 393, 400, 407 , 414, 421, 477, 484, 491, 498 , 505, 512, 57, 64, 71, 78, 85, 155, 162 , 169, 176, 246, 253 , 260, 267, 337, 344 , 351, 358, 428, 435 , 442, 449, 519, 526 , 533, 540, 92, 99, 106 183, 190 , 197 274, 281 , 288 365, 372 , 379 456, 463 , 470 547 659, 666, 673, 680, 687, 694, 701, 708, 715, 722, 729, 732 603, 610, 617, 624, 631, 638, 645, 652, 659, 666, 673, 680, 687 694, 701, 708, 715, 722, 729, 732 134, 141, 148,. 155, 162, 169, 232, 239 , 267, 274, 281, 288 , 309 316, 323, 330, 337, 344, 358, 365, 372 , 379, 386, 393, 400 , 407 414, 435, 442, 449, 456, 463, 470, 519 , 526, 533, 554, 561 , 582 645, 652, 659, 680, 687, 722, 729, 732 1 , 8 ,r 15, 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 709 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 168 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M4 (CONTINUED FROM PREVIOUS PAGE) C92747 T 105 NORMAL NO REMARKABLE OBSERVATIONS C92748 T 105 SKIN & PELAGE SORE/SCAB LIMB-FRONT-RIGHT NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) LIMB-FRONT-RIGHT WEEK 51 WART LIKE LESION(S) LIMB-FRONT-RIGHT WEEK 61 DOSE: 5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 113, 1 2 0 , 127, 176, 183, 190, 197, 204, 2 1 1 , 218, 225, 246, 253 260, 295, 302, 351, 421, 428, 477, 484, 491, 498, 505, 512, 540 547, 568, 575, 589, 596 372, 379, 386, 393, 400, 407, 414 1, 8 , 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 491, 498, 505, 512, 519 526, 533, 540, 547, 554, 561, 568, 575, 582, 589, 596, 603, 610 617, 624, 631, 638, 645, 652, 659, 666, 673, 680, 687, 694, 701 708, 715, 722, 729, 732 351, 358, 365 421, 428, 435, 442, 449, 456, 463, 470, 477, 484 710 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 169 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M4 DOSE: 5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92749 D 103 SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE NORMAL NO REMARKABLE OBSERVATIONS WART LIKE LESION(S) TAIL-MID WEEK 67 C92750 T 105 SKIN & PELAGE SORE/SCAB HEAD-MAXILLARY-RIGHT NORMAL NO REMARKABLE OBSERVATIONS 435, 442 1 , 8 ,r 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407 , 414, 421, 428, 449 , 456, 575, 582, 589 , 596 603, 610, 617, 624 , 631, 638, 645, 652 , 659, 666, 673, 680 , 687 694, 701, 708, 715 , 718 463, 470, 477, 484 , 491, 498, 505, 512 , 519, 526, 533, 540 , 547 554, 561, 568 624 1 , 8 ,r 15, 2 2 , 29, 36 , 43,r 50, 113, 1 2 0 , 127 134, 141, 148, 204, 2 1 1 , 218 225, 232, 239, 295, 302, 309 316, 323, 330, 386, 393, 400 407, 414, 421, 477, 484, 491 498, 505, 512, 568, 575, 582 589, 596, 603, 680, 687, 694 701, 708, 715, 57, 64, 71, 78, 85, 155, 162 , 169, 176, 246, 253 , 260, 267, 337, 344 , 351, 358, 428, 435 , 442, 449, 519, 526 , 533, 540, 631, 638 , 645, 652, 722, 729 , 732 92, 99, 106 183, 190 , 197 274, 281 , 288 365, 372 , 379 456, 463 , 470 547, 554 , 561 659, 666 , 673 711 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 170 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M4 (CONTINUED FROM PREVIOUS PAGE) C92750 T 105 MASS(ES) HEAD-MAXILLARY-RIGHT WEEK 88 DOSE: 5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 610, 617 712 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C92693 C92694 M4 680 98 TO 687 99 MASS(ES) DORSAL WEEK 98 694 100 MASS(ES) TO 708 102 DORSAL WEEK 98 715 103 MASS(ES) DORSAL WEEK 98 722 104 MASS(ES) DORSAL WEEK 98 729 105 MASS(ES) TO 733 105 DORSAL WEEK 98 498 72 TO 505 73 MASS(ES) VENTRAL -ABDOMINAL WEEK 72 M4 442 64 TO 456 66 MASS(ES) MOUTH WEEK 64 659 95 TO 687 99 MASS(ES) VENTRAL -ABDOMINAL-RIGHT WEEK 95 15 X 15MM 20 X 15MM 20 X 25MM 30 X3 0MM 40 X 40MM 15 X 15MM 10 X 10MM 10 X 10MM 713 Covance 6329-183 3M T-6295 PAGE: 171 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C92697 C92698 C92700 C92701 M4 400 58 TO 465 67 MASS(ES) DORSAL WEEK 58 M4 729 105 SKIN & PELAGE TO 732 105 OTHER M4 680 98 MASS(ES) TO 722 104 AXILLARY REGION-LEFT WEEK 98 729 105 MASS(ES) TO 733 105 AXILLARY REGION-LEFT WEEK 98 400 58 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 58 M4 323 47 MASS(ES) LIMB-FRONT-RIGHT WEEK 47 330 48 MASS(ES) LIMB-FRONT-RIGHT WEEK 47 337 49 MASS(ES) LIMB-FRONT-RIGHT WEEK 47 15 X 15MM BLACK HAIR COAT - PERINEAL 10 X 10MM 15 X 15MM 30 X 20MM 25 X 25MM 35 X 40MM 45 X 40 MM 714 Covance 6329-183 3M T-6295 PAGE: 172 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C92701 C92704 C92706 M4 344 50 TO 351 51 MASS(ES) LIMB-FRONT-RIGHT WEEK 47 358 52 MASS(ES) LIMB-FRONT-RIGHT WEEK 47 365 53 TO 365 53 MASS(ES) LIMB-FRONT-RIGHT WEEK 47 M4 659 95 TO 673 97 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 90 680 98 MASS(ES) TO 722 104 VENTRAL-ABDOMINAL-LEFT WEEK 90 729 105 MASS(ES) TO 732 105 VENTRAL-ABDOMINAL-LEFT WEEK 90 M4 568 82 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 82 568 82 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 82 65 X 60 MM 65 X 75MM 70 X 80MM 10 X 10MM 15 X 15MM 20 X 20MM 10 X 10MM 10 X 10MM 715 Covance 6329-183 3M T-6295 PAGE: 173 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C92706 C92710 C92716 C92717 M4 575 83 TO 596 86 MASS(ES) VENTRAL -ABDOMINAL-RIGHT WEEK 82 M4 428 62 TO 435 63 MASS(ES) DORSAL WEEK 54 M4 575 83 TO 582 84 MASS(ES) MOUTH WEEK 83 M4 680 98 MASS(ES) TO 722 104 DORSAL WEEK 98 596 86 TO 617 89 MASS(ES) VENTRAL -THORACIC-LEFT WEEK 86 624 90 TO 666 96 MASS(ES) VENTRAL -THORACIC-LEFT WEEK 86 673 97 MASS(ES) VENTRAL -THORACIC-LEFT WEEK 86 680 98 MASS(ES) TO 694 100 VENTRAL -THORACIC-LEFT WEEK 86 20 X 20MM 10 X 10MM 10 X 10MM 10 X 10MM 10 X 10MM 15 X 15MM 20 X 20MM 25 X 25MM 716 Covance 6329-183 3M T-6295 PAGE: 174 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C92717 C92720 M4 701 101 MASS(ES) TO 733 105 VENTRAL-THORACIC-LEFT WEEK 86 M4 526 76 MASS(ES) VENTRAL-ABDOMINAL WEEK 76 533 77 MASS(ES) VENTRAL-ABDOMINAL WEEK 76 540 78 MASS(ES) VENTRAL-ABDOMINAL WEEK 76 547 79 MASS(ES) VENTRAL-ABDOMINAL WEEK 76 554 80 MASS(ES) VENTRAL-ABDOMINAL WEEK 76 561 81 MASS(ES) VENTRAL-ABDOMINAL WEEK 76 568 82 MASS(ES) VENTRAL-ABDOMINAL WEEK 76 30 X 30MM 10 X 10MM 30 X 30MM 40 X 40MM 60 X 60MM 80 X 60MM 110 X 110MM 120 X 110MM 717 Covance 6329-183 3M T-6295 PAGE: 175 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 83 MASS(ES) 83 VENTRAL-ABDOMINAL WEEK 76 79 MASS(ES) 80 DORSAL WEEK 74 81 MASS(ES) 87 DORSAL WEEK 74 88 MASS(ES) 90 DORSAL WEEK 74 86 MASS(ES) 87 INGUINAL REGION-RIGHT WEEK 86 88 MASS(ES) 90 INGUINAL REGION-RIGHT WEEK 86 78 MASS(ES) 82 VENTRAL-ABDOMINAL WEEK 73 83 MASS(ES) 85 VENTRAL-ABDOMINAL WEEK 73 140 X 130MM 10 X 10MM 20 X 20MM 10 X 10MM 10 X 10MM 15 X 15MM 10 X 10MM 20 X 20MM 718 Covance 6329-183 3M T-6295 PAGE: 176 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS STUDY DAY WK CATEGORY KEYWORD QUALIFIER FREE- TEXT C92724 M4 C92729 M4 C92735 M4 C92737 M4 596 86 603 87 MASS(ES) VENTRAL-ABDOMINAL WEEK 73 610 659 95 CO CO MASS(ES) VENTRAL-ABDOMINAL WEEK 73 666 96 MASS(ES) 694 100 VENTRAL-ABDOMINAL WEEK 73 701 101 MASS(ES) 731 105 VENTRAL-ABDOMINAL WEEK 73 533 77 MASS(ES) INGUINAL REGION-LEFT WEEK 77 540 78 549 79 MASS(ES) INGUINAL REGION-LEFT WEEK 77 183 27 190 28 MASS(ES) VENTRAL-ABDOMINAL WEEK 27 554 80 637 91 MASS(ES) DORSAL WEEK 78 30 X 30MM 15 X 15MM 20 X 20MM 10 X 10MM 15 X 15MM 20 X 20MM 10 X 10MM 10 X 10MM 719 Covance 6329-183 3M T-6295 PAGE: 177 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C92740 C92742 C92745 M4 645 93 TO 659 95 M4 351 51 TO 379 55 386 56 TO 407 59 M4 512 74 TO 533 77 540 78 TO 547 79 554 80 TO 582 84 596 86 TO 624 90 316 46 TO 323 47 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 93 MASS(ES) DORSAL WEEK 51 MASS(ES) DORSAL WEEK 51 MASS(ES) DORSAL WEEK 74 MASS(ES) DORSAL WEEK 74 MASS(ES) DORSAL WEEK 74 MASS(ES) INGUINAL REGION-RIGHT WEEK 84 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 46 15 X 15MM 10 X 10MM 15 X 15MM 15 X 15MM 20 X 20MM 25 X 20MM 10 X 10MM 10 X 10MM 720 Covance 6329-183 3M T-6295 PAGE: 178 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C92745 M4 330 48 337 49 484 70 491 71 TO 533 77 540 78 547 79 554 80 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 46 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 46 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 70 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 70 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 70 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 70 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 70 15 X 15MM 10 X 10MM 10 X 10MM 20 X 20MM 25 X 25MM 30 X 30MM 20 X 15MM Covance 6329-183 3M T-6295 PAGE: 179 721 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 180 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M5 DOSE: 20 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92751 3 53 NORMAL NO REMARKABLE OBSERVATIONS C92752 1 C92753 T 4 NORMAL NO REMARKABLE OBSERVATIONS 105 APPEARANCE MALOCCLUSION MISSING TEETH THIN DISCHARGE GENITAL RED IN COLOR EXCRETION FEW FECES 1 , 8 ,r 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0, 127, 134 , 141, 148, 155, 162 , 169, 176, 183,. 190, 197 204, 2 1 1 , 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281, 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365-366 1 , 8 , 15 , 22 43, 50, 57, 64,r 71, 78, 85, 92, 99,r 106,r 113,r 1 2 0, 127,r 134, 141 148, 155 , 162, 169, 173, 176 , 183, 190, 197, 204, 2 1 1 , 218, 225 232, 239 , 246, 253, 260, 267 , 274, 281, 288, 295, 302, 309, 316 323, 330 , 337, 344, 351, 358 , 365, 372, 379, 386, 393, 400, 407 414, 421 , 428, 435, 442, 449 , 456, 463, 470, 477, 484, 491, 498 505, 512 , 519, 526, 533, 540 , 547, 554, 561, 568, 575, 582, 589 596, 603 , 610, 617, 624, 631 , 638, 645, 652, 659, 666, 673, 680 687, 694 , 701, 708, 715, 722 , 729, 733 589, 596 , 603, 610, 617, 624 , 631, 638, 645, 652, 659, 666, 673 680, 687 , 694, 701, 708, 715 , 722, 729, 733 173, 176, 183 666, 680, 687 173 722 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 181 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M5 (CONTINUED FROM PREVIOUS PAGE) C92753 T 105 SKIN & PELAGE SORE/SCAB VENTRAL-ABDOMINAL-RIGHT NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) INGUINAL REGION-RIGHT WEEK 90 C92754 3 53 APPEARANCE MALOCCLUSION NORMAL NO REMARKABLE OBSERVATIONS 3 53 NORMAL NO REMARKABLE OBSERVATIONS DOSE: 20 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 631, 638, 645, 652 1, 8 , 15, 22, 29, 36 624C 57, 64, 71, 78, 85, 92, 99, 106 1 , 8 ,r 15, 2 2 , 29, 36, 43,r 50, 113, 120 , 127, 134, 141, 148 , 155 162, 169, 176,. 183, 190, 197, 204, 211 , 218, 225, 232, 239 , 246 253, 260, 267,. 274, 281, 288, 295, 302 , 309, 316, 323, 330 , 337 344, 351, 358, 365-366 1 , 8 ,r 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218,. 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365- 366 723 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 182 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M5 DOSE: 20 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92756 M 88 BEHAVIOR HYPOACTIVE <UNSPECIFIED> EXCRETION FEW FECES SKIN & PELAGE SORE/SCAB DORSAL NORMAL NO REMARKABLE OBSERVATIONS C92757 T MASS(ES) DORSAL WEEK 84 105 APPEARANCE MALOCCLUSION 614 590, 596, 610, 614 582, 589- 590, 596, 603, 610, 614 1 , 8 ,r 15, 2 2 , 29, 36, 43 , 50, 113, 1 2 0, 127, 134 , 141, 148, 204, 2 1 1 , 218, 225 , 232, 239, 295, 302, 309, 316 , 323, 330, 386, 393, 400, 407 , 414, 421, 477, 484, 491, 498 , 505, 512, 568, 575 57, 64, 71, 78, 85, 155, 162 , 169, 176, 246, 253 , 260, 267, 337, 344 , 351, 358, 428, 435 , 442, 449, 519, 526 , 533, 540, 92, 99, 106 183, 190 , 197 274, 281 , 288 365, 372 , 379 456, 463 , 470 547, 554 , 561 582, 589- 590, 596, 603, 610, 614 309, 400, 505, 596, 687, 316, 407, 512, 603, 694, 323, 414, 519, 610, 701, 330, 421, 526, 617, 708, 337, 428, 533, 624, 715, 344, 435, 540, 631, 722, 351, 442, 547, 638, 729, 358, 463, 554, 645, 731 365, 470, 561, 652, 372, 477, 568, 659, 379, 386, 484, 491, 575, 582, 666, 673, 393 498 589 680 724 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 183 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M5 (CONTINUED FROM PREVIOUS PAGE) C92757 T 105 NORMAL NO REMARKABLE OBSERVATIONS C92758 M MASS(ES) DORSAL WEEK 98 62 APPEARANCE THIN BEHAVIOR HYPOACTIVE <UNSPECIFIED> EXCRETION FEW FECES SKIN & PELAGE ROUGH HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS DOSE: 20 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 1 , 8 , 15, 2 2 , 29, 36, 43, 50, 57, 64, 71, 78,, 85, 92, 99, 106 113, 1 2 0, 127,. 134, 141, 148, 155, 162, 169, 176, 183,. 190, 197 204, 2 1 1 , 218,. 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 449, 456 680C,, 687C!, 694C, 701C, 708C, 715C , 722C, 729C, 731C 400, 407, 414, 421, 428 428 393, 414, 421, 428 414, 421, 428 1 , 8 ,r 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386 725 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 184 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M5 DOSE: 20 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92759 1 C92760 M 4 NORMAL NO REMARKABLE OBSERVATIONS 74 APPEARANCE THIN EXCRETION FEW FECES EYES RED DISCHARGE EYES NORMAL NO REMARKABLE OBSERVATIONS C92761 T APPEARANCE MALOCCLUSION EXCRETION FEW FECES EYES PROTRUDING EYES 1, 8 , 15, 22 512 503, 505, 512 512 1 , 8 ,r 15, 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498 596, 603, 610, 617, 624, 631, 638, 645 , 652, 659, 666, 673 , 680 687, 694, 701, 708 603, 610 726 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 185 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M5 (CONTINUED FROM PREVIOUS PAGE) C92761 T 105 NORMAL NO REMARKABLE OBSERVATIONS C92762 T 105 SKIN & PELAGE SORE/SCAB VENTRAL-ABDOMINAL NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) VENTRAL-ABDOMINAL WEEK 68 DOSE: 20 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 1, 8 15, 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582, 589, 715, 722, 729, 732 491, 498, 505 1 , 8 ,r 15, 2 2 , 29, 36 , 4 3, 50 , 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0, 127, 134, 141 , 148 , 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225, 232 , 239 , 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316, 323 , 330 , 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414 , 421 , 428, 435 , 442, 449, 456, 463 , 512 519, 526, 533, 540, 547 , 554 , 561, 568 , 575, 582, 589, 596 , 603 610, 617, 624, 631, 638 , 645 , 652, 659 , 666, 673, 680, 687 , 694 701, 708, 715, 722, 729 , 731 470, 477, 484C , 491C , 4 98C, 505 727 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 186 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M5 DOSE: 20 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92763 M 94 APPEARANCE OBES SWOLLEN PAW-HIND-RIGHT PAWS-HIND EXCRETION FEW FECES SKIN & PELAGE BROWN HAIR COAT PERINEAL AREA YELLOW HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS C92764 M 76 APPEARANCE HUNCHED THIN BEHAVIOR ATAXIC <UNSPECIFIED> HYPOACTIVE <UNSPECIFIED> 596, 603, 610 631 638, 645, 652-653 652-653 653 638, 645, 652-653 1 22, 8,r 15, , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 120113, , 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 211204, , 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582, 589, 617, 624 530 505, 512, 519, 526, 530 526, 530 530 728 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 187 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M5 (CONTINUED FROM PREVIOUS PAGE) C92764 M 76 BEHAVIOR COLD TO TOUCH LIMBS-ALL TAIL EXCRETION FEW FECES NORMAL NO REMARKABLE OBSERVATIONS C92765 2 C92766 D 14 NORMAL NO REMARKABLE OBSERVATIONS 101 EYES RED DISCHARGE EYE-RIGHT SKIN & PELAGE SORE/SCAB VENTRAL-ABDOMINAL-LEFT NORMAL NO REMARKABLE OBSERVATIONS DOSE: 20 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 530 530 512, 519 526, 530 1 , 8 , 15 113, 120 204, 211 295, 302 386, 393 477, 484 2 2 , 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 127,. 134, 141, 148, 155, 162 , 169, 176, 183 , 190 , 197 218,. 225, 232, 239, 246, 253 , 260, 267, 274 , 281 , 288 309,. 316, 323, 330, 337, 344 , 351, 358, 365 , 372 , 379 400,. 407, 414, 421, 428, 435 , 442, 449, 456 , 463 , 470 491, 498 1 , 8 , 15 2 2 , 29, 36, 43, 50, 57, 64, 71, 78, 85, 92- 93 666 358 1 , 8 ,r 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 365 , 372, 379, 386, 393 , 400 729 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 188 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M5 (CONTINUED FROM PREVIOUS PAGE) C92766 D 101 NORMAL NO REMARKABLE OBSERVATIONS C92767 D MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 50 RECTUM WEEK 99 99 APPEARANCE OBES EXCRETION FEW FECES SKIN & PELAGE SORE/SCAB LATERAL-ABDOMINAL-LEFT VENTRAL-ABDOMINAL-RIGHT NORMAL NO REMARKABLE OBSERVATIONS DOSE: 20 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 407, 414, 421, 428, 435, 442, 449, 456, 463, 470, 477, 484, 491 498, 505, 512, 519, 526, 533, 540, 547, 554, 561, 568, 575, 582 589, 596, 603, 610, 617, 624, 631, 638, 645, 652, 659, 673, 680 344, 351 687, 694, 701, 705 463, 470, 477, 484, 491, 498, 505, 512, 519, 526, 533, 540, 547 554, 561, 568, 575, 582, 589, 596, 603, 610, 638, 645, 652, 659 666, 673, 680, 687, 691 673 519, 526, 533,. 540, 547, 554, 561, 568 , 575, 582, 589, 596 , 603 610, 617, 624, 631, 638, 645, 652, 659 , 666, 673, 680, 687 , 691 659, 666, 673, 680, 687, 691 1 , 8 ,r 15, 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456 730 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 189 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M5 DOSE: 20 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92768 M 95 SKIN & PELAGE BLACK SKIN DORSAL SORE/SCAB VENTRAL-ABDOMINAL VENTRAL-ABDOMINAL-RIGHT NORMAL NO REMARKABLE OBSERVATIONS C92769 D MASS(ES) DORSAL WEEK 85 INGUINAL REGION-RIGHT WEEK 90 VENTRAL-ABDOMINAL WEEK 77 103 APPEARANCE LIMITED USE PAWS-ALL SKIN & PELAGE BLACK SKIN VENTRAL-ABDOMINAL 660 540, 547 631, 638, 645, 652,659-660 1 , 8 , 15, 2 2 , 29, 36, 43,r 50, 113, 1 2 0 , 127, 134 , 141, 148, 204, 2 1 1 , 218, 225 , 232, 239, 295, 302, 309, 316 , 323, 330, 386, 393, 400, 407 , 414, 421, 477, 484, 491, 498 , 505, 512, 57, 64, 71 , 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 554, 561, 92, 99, 106 183 , 190 , 197 274 , 281 , 288 365 , 372 , 379 456 , 463 , 470 568 , 575 , 582 589, 596C, 603C, 610C, 617C, 624C, 631C, 638C , 645C, 652C 659- 660C 624 533C 715, 721 624 731 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 190 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M5 (CONTINUED FROM PREVIOUS PAGE) C92769 D 103 SKIN & PELAGE SORE/SCAB VENTRAL-ABDOMINAL NORMAL NO REMARKABLE OBSERVATIONS C92770 D MASS(ES) INGUINAL REGION-LEFT WEEK 86 INGUINAL REGION-RIGHT WEEK 86 VENTRAL-ABDOMINAL WEEK 89 101 APPEARANCE LIMITED USE LIMBS-ALL PAW-HIND-LEFT SWOLLEN LIMBS-HIND PAW-HIND-LEFT DOSE: 20 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 631, 638 1 , 8 ,r 15, 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582, 589, 645, 652, 659, 666 , 673, 680, 687, 694 , 701 708 596C, 603C, 610C 596C, 603C, 610C 617C, 624C 694, 701-702 539-540, 547, 554, 561, 568, 575, 582 694, 701-702 539-540, 547, 554, 561, 568, 575, 582 732 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 191 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M5 (CONTINUED FROM PREVIOUS PAGE) C92770 D 101 BEHAVIOR HYPOACTIVE <UNSPECIFIED> SKIN & PELAGE YELLOW HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS C92771 M MASS(ES) PAW-FRONT-LEFT WEEK 97 81 BEHAVIOR HYPOACTIVE <UNSPECIFIED> EXCRETION NO FECES EYES RED DISCHARGE EYES DOSE: 20 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 701-702 701-702 1 , 8 ,r 15, 2 2 , 29, 36, 43 , 50, 113, 1 2 0 , 127, 134 , 141, 148, 204, 2 1 1 , 218, 225 , 232, 239, 295, 302, 309, 316 , 323, 330, 386, 393, 400, 407 , 414, 421, 477, 484, 491, 498 , 505, 512, 617, 624, 631, 638 , 645, 652, 57, 64, 71, 78, 85, 155, 162 , 169, 176, 246, 253 , 260, 267, 337, 344 , 351, 358, 428, 435 , 442, 449, 519, 526 , 533, 589, 659, 666 92, 99, 106 183, 190 , 197 274, 281 , 288 365, 372 , 379 456, 463 , 470 596, 603 , 610 673, 680, 687,. 694 , 701- 702 567 567 561, 567 733 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 192 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M5 (CONTINUED FROM PREVIOUS PAGE) C92771 M 81 SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE VENTRAL-ABDOMINAL ROUGH HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS M 52 NORMAL NO REMARKABLE OBSERVATIONS SURGERY PERFORMED BY KATHY EWERS ANTIBIOTIC ADMINISTERED 0.5 ML DOSE: 20 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 561, 567 561, 567 561, 567 1 , 8 ,r 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407 , 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498 , 505, 512, 519, 526 , 533, 540, 547, 554 1 , 8 ,r 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 360 359 359 734 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 193 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M5 DOSE: 20 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92773 D 105 APPEARANCE SWOLLEN PAW-HIND-RIGHT VENTRAL-ABDOMINAL-LEFT THIN NORMAL NO REMARKABLE OBSERVATIONS C92774 T 105 SKIN & PELAGE SORE/SCAB VENTRAL-ABDOMINAL NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) VENTRAL-ABDOMINAL WEEK 83 428, 435, 442, 449, 456, 463 715, 722, 729, 731 589, 596, 603, 610 1 , 8 ,r 15, 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 470, 477 , 484, 491, 498, 505 , 512 519, 526, 533,. 540, 547, 554, 561, 568 , 575, 582, 617, 624 , 631 638, 645, 652, 659, 666, 673, 680, 687 , 694, 701, 708 617 1, 8 , 15 113, 120 204, 211 295, 302 386, 393 477, 484 568, 624 708, 715 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 631,. 638, 645, 652, 659, 666 , 673, 680, 687, 694 , 701 722,. 729, 733 575, 582 589,. 596, 603, 610 735 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 194 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M5 DOSE: 20 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92775 T 105 APPEARANCE MISSING TEETH EXCRETION FEW FECES SKIN & PELAGE SORE/SCAB DORSAL NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) DORSAL WEEK 72 344, 351 729, 732 722, 729, 732 1 , 8 ,r 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 358 , 365, 372, 379, 386 , 393 400, 407, 414, 421 , 428, 435, 442, 449 , 456, 463, 470, 477 , 484 491 498, 505, 512, 519C, 526C, 533C, 540C, 547C, 554C, 561C, 568C 575C, 582C, 589C, 596C, 603C, 610C, 617C, 624C, 631C, 638C, 645C 652C, 659C, 666C, 673C, 680C, 687C, 694C, 701C, 708C, 715C, 722C 729C, 732C 736 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 195 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M5 DOSE: 20 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92776 T 105 DISCHARGE GENITAL RED IN COLOR NORMAL NO REMARKABLE OBSERVATIONS C92777 2 C92778 3 WART LIKE LESION(S) MOUTH WEEK 102 14 NORMAL NO REMARKABLE OBSERVATIONS 53 NORMAL NO REMARKABLE OBSERVATIONS SURGERY PERFORMED BY JOHN VAN BERKUM 78 1 , 8 ,r 15, 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 85,r 92, 99, 106, 113 1 2 0 , 127, 134 141, 148, 155, 162, 169 , 176, 183, 190, 197, 204 2 1 1 , 218, 225 232, 239, 246, 253, 260 , 267, 274, 281, 288, 295 302, 309, 316 323, 330, 337, 344, 351 , 358, 365, 372, 379, 386 393, 400, 407 414, 421, 428, 435, 442 , 449, 456, 463, 470, 477 484, 491, 498 505, 512, 519, 526, 533 , 540, 547, 554, 561, 568 575, 582, 589 596, 603, 610, 617, 624 , 631, 638, 645, 652, 659 666, 673, 680 687, 694, 701 708, 715, 722 729, 732 1, 8 , 15, 22, 29, 36, 43, 50 57, 64, 71, 78 85 92-93 1 , 8 ,r 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190, 197 204, 2 1 1 , 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281, 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365-366 359 737 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 196 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M5 (CONTINUED FROM PREVIOUS PAGE) C92778 3 53 ANTIBIOTIC ADMINISTERED 0.5 ML C92779 1 4 NORMAL NO REMARKABLE OBSERVATIONS C92780 T 105 APPEARANCE HUNCHED EXCRETION FEW FECES NORMAL NO REMARKABLE OBSERVATIONS M 77 APPEARANCE THIN TREMORS ENTIRE BODY BEHAVIOR HYPOACTIVE <UNSPECIFIED> DOSE: 20 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 359-360 1, 8 , 15, 22 729, 733 722, 729, 733 1 , 8 ,r 15, 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582, 589, 596, 603, 610, 617 , 624, 631, 638, 645 , 652 659, 666, 673, 680, 687, 694, 701, 708 , 715 498, 503, 505, 507, 512, 519, 526, 533 , 535 535 535 738 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 197 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M5 (CONTINUED FROM PREVIOUS PAGE) C92781 M 77 BEHAVIOR COLD TO TOUCH ENTIRE BODY EXCRETION FEW FECES SKIN & PELAGE PALE ENTIRE BODY NORMAL NO REMARKABLE OBSERVATIONS T 105 APPEARANCE OBES SKIN & PELAGE SORE/SCAB VENTRAL-ABDOMINAL-RIGHT NORMAL NO REMARKABLE OBSERVATIONS DOSE: 20 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 533, 535 503, 505, 507,. 512, 519, 526, 533, 535 519, 526, 533, 535 1 , 8 ,r 15, 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491 596, 603, 610 729, 732 1 , 8 ,r 15, 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 739 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 198 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M5 (CONTINUED FROM PREVIOUS PAGE) C92782 T 105 NORMAL NO REMARKABLE OBSERVATIONS C92783 M MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 103 79 APPEARANCE THIN BEHAVIOR RECUMBENT STERNAL COLD TO TOUCH ENTIRE BODY EXCRETION NO FECES EYES RED DISCHARGE EYE-RIGHT RESPIRATION AUDIBLE LABORED NORMAL NO REMARKABLE OBSERVATIONS DOSE: 20 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 477, 484, 491, 498, 505, 512, 519, 526, 533, 540, 547, 554, 561 568, 575, 582, 589, 617, 624, 631, 638, 645, 652, 659, 666, 673 680, 687, 694, 701, 708 715C, 722C, 729, 732 547 547 547 547 512, 519, 526, 533, 540, 547 547 547 1, 8 , 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 740 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 199 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M5 (CONTINUED FROM PREVIOUS PAGE) C92783 M 79 NORMAL NO REMARKABLE OBSERVATIONS C92784 M 80 BEHAVIOR HYPOACTIVE <UNSPECIFIED> EXCRETION FEW FECES NONFORMED FECES NORMAL NO REMARKABLE OBSERVATIONS C92785 D 98 NORMAL NO REMARKABLE OBSERVATIONS DOSE: 20 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 204, 295, 386, 477, 211, 218, 302, 309, 393, 400, 484, 491, 225, 316, 407, 498, 232, 323, 414, 505 239, 330, 421, 246, 337, 428, 253, 344, 435, 260, 351, 442, 267, 358, 449, 274, 365, 456, 281, 372, 463, 288 379 470 556 555-556 555-556 1 22, 8,r 15, , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 120113, , 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 211204, , 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407 , 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498 , 505, 512, 519, 526 , 533, 540, 547, 554 1 22, 8,r 15, , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 120113, , 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 211204, , 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582, 589, 596, 603, 610, 617 , 624, 631, 638, 645 , 652 659, 666, 673, 680, 686 741 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 200 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M5 DOSE: 20 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92786 T 105 EXCRETION NONFORMED FECES NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 44 D 47 APPEARANCE LIMITED USE LIMB-HIND-LEFT LIMBS-HIND SWOLLEN LIMB-HIND-LEFT NORMAL NO REMARKABLE OBSERVATIONS 729 1 , 8 , 15, 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 323, 330, 337, 344, 351, 358, 365 , 372, 379, 386, 393 , 400 407, 414, 421,. 428, 435, 442, 449, 456 , 463, 470, 477, 484 , 491 498, 505, 512, 519, 526, 533, 540, 547 , 554, 561, 568, 575 , 582 589, 596, 603,. 610, 617, 624, 631, 638 , 645, 652, 659, 666 , 673 680, 687, 694, 701, 708, 715, 722, 731 302C,, 309C , 316C 302, 309, 316 323, 328 309 1 , 8 , 15, 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 2o9ne5 742 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 201 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M5 DOSE: 20 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92788 T 105 APPEARANCE MALOCCLUSION NORMAL NO REMARKABLE OBSERVATIONS C92789 2 C92790 3 14 APPEARANCE MALOCCLUSION NORMAL NO REMARKABLE OBSERVATIONS 53 NORMAL NO REMARKABLE OBSERVATIONS 106, 113 1 , 8 ,r 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 120 127, 134, 141 148 , 155, 162, 169, 176 , 183, 190, 197, 204 , 211 218, 225, 232 239 , 246, 253, 260, 267 , 274, 281, 288, 295 , 302 309, 316, 323 330 , 337, 344, 351, 358 , 365, 372, 379, 386 , 393 400, 407, 414 421 , 428, 435, 442, 449 , 456, 463, 470, 477 , 484 491, 498, 505 512 , 519, 526, 533, 540 , 547, 554, 561, 568 , 575 582, 589, 596 603 , 610, 617, 624, 631 , 638, 645, 652, 659 , 666 673, 680, 687 694 , 701, 708, 715, 722 , 729, 733 92-93 1 , 8 , 15 2 2 , 29, 36, 43,, 50, 57, 64, 71, 78, 85 1 , 8 , 15 113, 120 204, 211 295, 302 2 2 , 29, 36, 43,, 50, 57, 64, 71, 78, 85, 92, 99, 106 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190, 197 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281, 288 309, 316 , 323, 330, 337, 344 , 351, 358, 365-366 743 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 202 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M5 DOSE: 20 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92791 T 105 SKIN & PELAGE SORE/SCAB DORSAL NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) DORSAL WEEK 93 C92792 T 105 SKIN & PELAGE SORE/SCAB AXILLARY REGION-LEFT NORMAL NO REMARKABLE OBSERVATIONS 722, 729, 731 1 , 8 , 15, 2 2 , 29, 36, 43 , 50, 57, 64, 71, 78, 85 , 92, 99, 106 113, 1 2 0 , 127,. 134, 141, 148, 155, 162, 169, 176 , 183, 190 , 197 204, 2 1 1 , 218,. 225, 232, 239, 246, 253, 260, 267 , 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344, 351, 358 , 365, 372 , 379 386, 393, 400,. 407, 414, 421, 428, 435, 442, 449 , 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526, 533, 540 , 547, 554 , 561 568, 575, 582,. 589, 596, 603, 610, 617, 624, 631 , 638 645C, 652C , 659C, 666C, 673C, 680C , 687C, 694C, 701C, 708C , 715C 722C, 72 9C, 731C 729, 732 1 , 8 ,r 15, 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582, 589, 596, 603, 610, 617 , 624, 631, 638, 645 , 652 659, 666, 673, 680, 687, 694 744 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 203 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M5 (CONTINUED FROM PREVIOUS PAGE) C92792 T 105 MASS(ES) AXILLARY REGION-LEFT WEEK 101 VENTRAL-ABDOMINAL WEEK 104 C92793 1 4 NORMAL NO REMARKABLE OBSERVATIONS C92794 D 58 APPEARANCE THIN EXCRETION FEW FECES EYES RED DISCHARGE EYE-RIGHT SKIN & PELAGE BROWN HAIR COAT VENTRAL-ABDOMINAL NORMAL NO REMARKABLE OBSERVATIONS DOSE: 20 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 701C, 708C, 715C, 722C, 729C, 732C 722, 729, 732 1, 8 , 15, 22 400, 402 393, 400, 402 204, 211, 225, 232, 365, 372, 379, 386, 393, 400, 402 400, 402 1 , 8 , 15 113, 120 218, 239 323, 330 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 127, 134 , 141, 148, 155,. 162, 169, 176, 183, 190 , 197 246, 253 , 260, 267, 274,. 281, 288, 295, 302, 309 , 316 337, 344 , 351, 358 745 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 204 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M5 DOSE: 20 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92795 3 53 NORMAL NO REMARKABLE OBSERVATIONS SURGERY PERFORMED BY KATHY EWERS ANTIBIOTIC ADMINISTERED 0.5 ML 3 53 SKIN & PELAGE ROUGH HAIR COAT SORE/SCAB LIMB-FRONT-RIGHT NORMAL NO REMARKABLE OBSERVATIONS SURGERY PERFORMED BY JOHN VAN BERKUM ANTIBIOTIC ADMINISTERED 0.5 ML 1 , 8 , 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127, 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316, 323, 330, 337, 344 , 351, 358, 365-366 359 359- 360 239, 323, 330, 337, 344, 351, 358 92 1 , 8 , 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 99, 106, 113 1 2 0 , 127, 134, 141, 148, 155, 162, 169 , 176, 183, 190, 197 , 204 2 1 1 , 218, 225, 232, 246, 253, 260, 267 , 274, 281, 288, 295 , 302 309, 316, 365-366 359 359-360 746 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 205 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M5 DOSE: 20 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92797 M 94 APPEARANCE LIMITED USE LIMB-HIND-RIGHT MALOCCLUSION MISSING TEETH NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 81 1 4 NORMAL NO REMARKABLE OBSERVATIONS 2 14 NORMAL NO REMARKABLE OBSERVATIONS 653 596, 603, 610 596 1 , 8 , 15, 2 2 , 29, 36, 43 , 50, 57, 64, 71 , 78, 85 , 92, 99, 106 113, 1 2 0 , 127,. 134, 141, 148, 155, 162, 169, 176 , 183, 190 , 197 204, 2 1 1 , 218,. 225, 232, 239, 246, 253, 260, 267 , 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344, 351, 358 , 365, 372 , 379 386, 393, 400,. 407, 414, 421, 428, 435, 442, 449 , 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526, 533, 540 , 547, 554 561C, 568C , 575C, 582C, 589C, 596C , 603C , 610C, 617C, 624C , 631C 638C, 645C 652-653C 1 , 8 , 15, 22 1 , 8 , 15, 22, 29, 36, 43, 50, 57, 64, 71, 78 747 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 206 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M5 DOSE: 20 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92800 T 105 DISCHARGE GENITAL RED IN COLOR NORMAL NO REMARKABLE OBSERVATIONS C92801 D 57 NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 56 VENTRAL-ABDOMINAL-RIGHT WEEK 53 50 1 , 8 ,r 15, 2 2 , 29, 36 , 43,r 57, 64, 71, 78 , 85,r 92, 99, 106, 113 1 2 0 , 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197, 204 2 1 1 , 218, 225,. 232, 239, 246, 253, 260, 267, 274, 281, 288, 295 302, 309, 316,. 323, 330, 337, 344, 351, 358, 365, 372, 379, 386 393, 400, 407,. 414, 421, 428, 435, 442, 449, 456, 463, 470, 477 484, 491, 498, 505, 512, 519, 526, 533, 540, 547, 554, 561, 568 575, 582, 589,. 596, 603, 610, 617, 624, 631, 638, 645, 652, 659 666, 673, 680, 687, 694, 701, 708, 715, 722, 729, 731 1 , 8 ,r 15, 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127,. 134, 141, 148, 155, 162 , 169, 176, 183,. 190, 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281, 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358 386C, 393C 365C, 372C, 379C, 386C, 393C 748 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 207 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M5 DOSE: 20 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92802 T 105 NORMAL NO REMARKABLE OBSERVATIONS C92803 T 105 NORMAL NO REMARKABLE OBSERVATIONS C92804 T 105 SKIN & PELAGE SORE/SCAB VENTRAL-ABDOMINAL NORMAL NO REMARKABLE OBSERVATIONS 1 , 8 ,r 15, 2 2 , 29, 36 , 43,r 50, 113, 1 2 0 , 127,. 134, 141, 148, 204, 2 1 1 , 218, 225, 232, 239, 295, 302, 309,. 316, 323, 330, 386, 393, 400, 407, 414, 421, 477, 484, 491, 498, 505, 512, 568, 575, 582, 589, 596, 603, 659, 666, 673, 680, 687, 694, 57, 64, 71, 78, 85, 155, 162 , 169, 176, 246, 253 , 260, 267, 337, 344 , 351, 358, 428, 435 , 442, 449, 519, 526 , 533, 540, 610, 617 , 624, 631, 701, 708 , 715, 722, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 638, 645, 652 729, 731 1 , 8 ,r 15, 2 2 , 29, 36 , 43,r 50, 113, 1 2 0 , 127,. 134, 141, 148, 204, 2 1 1 , 218, 225, 232, 239, 295, 302, 309,. 316, 323, 330, 386, 393, 400, 407, 414, 421, 477, 484, 491, 498, 505, 512, 568, 575, 582, 589, 596, 603, 659, 666, 673, 680, 687, 694, 57, 64, 71, 78, 85, 155, 162 , 169, 176, 246, 253 , 260, 267, 337, 344 , 351, 358, 428, 435 , 442, 449, 519, 526 , 533, 540, 610, 617 , 624, 631, 701, 708 , 715, 722, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 638, 645, 652 729, 733 561 1 , 8 ,r 15, 2 2 , 29, 36 , 43,r 50, 113, 1 2 0 , 127,. 134, 141, 148, 204, 2 1 1 , 218, 225, 232, 239, 295, 302, 309,. 316, 323, 330, 400, 407, 414, 421, 428, 435, 491, 498, 505,. 512, 519, 526, 589, 596, 603,. 610, 617, 624, 57, 64, 71, 78, 85, 155, 162 , 169, 176, 246, 253 , 260, 267, 337, 344 , 351, 358, 442, 449 , 456, 463, 533, 540 , 547, 554, 631, 638 , 645, 652, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 470, 477, 484 568, 575, 582 659, 666, 673 749 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 208 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M5 (CONTINUED FROM PREVIOUS PAGE) C92804 T 105 NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 56 C92805 M 72 APPEARANCE THIN BEHAVIOR HYPOACTIVE <UNSPECIFIED> EYES RED DISCHARGE EYE-LEFT SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE SORE/SCAB PAW-HIND-LEFT VENTRAL-ABDOMINAL-LEFT NORMAL NO REMARKABLE OBSERVATIONS DOSE: 20 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 680, 687, 694, 701, 708, 715, 722, 729, 731 386C, 393C 498, 500 498, 500 500 484, 491 498 463, 470 477, 484 , 491, 498, 500 456, 463 1 , 8 , 15 113, 120 204, 211 295, 302 386, 393 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 400, 407 , 414, 421, 428, 435 , 442 750 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 209 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M5 (CONTINUED FROM PREVIOUS PAGE) C92805 M 72 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 65 C92806 M 99 APPEARANCE MALOCCLUSION MISSING TEETH THIN EXCRETION FEW FECES NO FECES RESPIRATION IRREGULAR LABORED SKIN & PELAGE SORE/SCAB VENTRAL-ABDOMINAL-RIGHT DOSE: 20 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 449 57, 64, 71, 78, 85, 92, 99, 106, 113, 1 2 0 , 127, 134, 141, 148 155, 162 , 169, 176, 183, 190 , 197 , 204 , 211 , 218 , 225 , 232 , 239 246, 253 , 260, 267, 274, 281 , 288 , 295 , 302 , 309 , 316 , 323 , 330 337, 344 , 351, 358, 365, 372 , 379 , 386 , 393 , 400 , 407 , 414 , 421 428, 435 , 442, 449, 456, 463 , 470 , 477 , 484 , 491 , 498 , 505 , 512 519, 526 , 533, 540, 547, 554 , 561 , 568 , 575 , 582 , 589 , 596 , 603 610, 617 , 624, 631, 638, 645 , 652 , 659 , 666 , 673 , 680 , 687 , 691 267, 358, 449, 540, 631, 687, 274 , 281, 365 , 372, 456 , 463, 547 , 554, 638 , 645, 691 288, 379, 470, 561, 652, 295, 386, 477, 568, 659, 302 , 309 , 316 , 323 , 330 , 337 , 344 , 351 393 , 400 , 407 , 414 , 421 , 428 , 435 , 442 484 , 491 , 498 , 505 , 512 , 519 , 526 , 533 575 , 582 , 589 , 596 , 603 , 610 , 617 , 624 666 , 673 , 680 , 687 , 691 680, 687 691 687, 691 680, 687, 691 596, 680, 687, 691 751 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 210 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M5 (CONTINUED FROM PREVIOUS PAGE) C92806 M 99 NORMAL NO REMARKABLE OBSERVATIONS C92807 D 86 EXCRETION FEW FECES SKIN & PELAGE SORE/SCAB VENTRAL-ABDOMINAL NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) VENTRAL-ABDOMINAL WEEK 39 T 105 SKIN & PELAGE SORE/SCAB AXILLARY REGION-LEFT NORMAL NO REMARKABLE OBSERVATIONS DOSE: 20 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 1, 8 , 15, 22, 29, 36, 43, 50 596-597 295 1 , 8 ,r 15, 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190, 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253 , 260, 302, 309, 316, 323 330, 337, 344, 351, 358, 365, 372, 379 , 386, 393, 400, 407, 414 421, 428, 435,. 442, 449, 456, 463, 470 , 477, 484, 491, 498, 505 512, 519, 526,. 533, 540, 547, 554, 561 , 568, 575, 582, 589 267C, 274C, 281C, 288C 729, 731 1 , 8 , 15 113, 120 204, 211 295, 302 386, 393 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190, 197 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281, 288 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372, 379 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463, 470 752 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 211 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M5 (CONTINUED FROM PREVIOUS PAGE) C92808 T 105 NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) AXILLARY REGION-LEFT WEEK 76 WEEK 103 INGUINAL REGION-LEFT WEEK 98 VENTRAL-ABDOMINAL-LEFT WEEK 98 T 105 SKIN & PELAGE SORE/SCAB VENTRAL-ABDOMINAL-RIGHT NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) AXILLARY REGION-LEFT WEEK 85 INGUINAL REGION-LEFT WEEK 86 DOSE: 20 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 477, 484, 491, 498, 505, 512, 519 526, 533, 540, 547, 554, 561, 568, 575, 582, 589, 596, 603 , 610 617, 624, 631, 638, 645, 652, 659, 666, 673, 680 715, 722, 72 9C , 731C 680, 687, 694, 701, 708, 715, 722, 72 9C, 731C 680, 687, 694, 701, 708, 715, 722, 729, 731 484, 491, 498, 505, 610, 617 1 , 8 ,r 15, 2 2 , 29, 36 , 43,r 50, 57, 64, 71 , 78, 85, 92, 99, 106 113, 1 2 0 , 127, 134, 141, 148, 155, 162, 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253, 260, 267, 274, 281 , 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463 , 470 512, 519, 526, 533, 540, 547, 554, 561, 568, 575, 582 58 9C, 596C, 603C, 610C, 617C, 624C, 631C, 638C, 645C, 652C, 659C 666C, 673C, 680C, 687C, 694C, 701C, 708C, 715C, 722C, 729C, 733C 596C, 603C 753 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 212 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M5 (CONTINUED FROM PREVIOUS PAGE) C92809 T 105 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 69 C92810 M 97 APPEARANCE THIN BEHAVIOR HYPOACTIVE <UNSPECIFIED> EXCRETION FEW FECES EYES SQUINTED PERI-ORBITAL NORMAL NO REMARKABLE OBSERVATIONS DOSE: 20 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 477C, 484C, 491C, 498C 659, 666, 673 673 656, 659, 666, 673 673 1 22, 8,r 15, , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 120113, , 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 211204, , 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582, 589, 596, 603, 610, 617 , 624, 631, 638, 645 , 652 754 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 213 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M5 DOSE: 20 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92811 M 36 APPEARANCE LIMITED USE LIMBS-HIND EXCRETION FEW FECES NORMAL NO REMARKABLE OBSERVATIONS C92812 2 C92813 M 14 NORMAL NO REMARKABLE OBSERVATIONS 100 APPEARANCE THIN BEHAVIOR HYPOACTIVE <UNSPECIFIED> EXCRETION FEW FECES EYES SQUINTED PERI-ORBITAL SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE 250 250 1 , 8 , 15, 2 2 , 29, 36 , 43,, 50, 57, 64, 71 , 78, 85, 92, 99, 106 113, 1 2 0, 127, 134 , 141, 148, 155, 162 , 169, 176, 183 , 190, 197 204, 2 1 1 , 218, 225 , 232, 239, 246 1 , 8 , 15, 2 2 , 29, 36 , 43,, 50, 57, 64, 71 , 78, 85, 92- 93 694 694 694 694 576 755 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 214 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M5 (CONTINUED FROM PREVIOUS PAGE) C92813 M 100 SKIN & PELAGE RED HAIR COAT SHOULDER-RIGHT SORE/SCAB DORSAL DORSAL-THORACIC-LEFT HEAD-ENTIRE PAW-HIND-RIGHT NORMAL NO REMARKABLE OBSERVATIONS C92814 T MASS(ES) DORSAL WEEK 87 WEEK 99 105 APPEARANCE MALOCCLUSION SKIN & PELAGE SORE/SCAB VENTRAL-ABDOMINAL-RIGHT DOSE: 20 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 576 638, 631 561, 638, 645, 568, 645, 652, 659, 575-576 652, 659, 666, 673, 666, 673, 680, 680, 687, 687, 694 694 1 , 8 ,r 15, 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 582 589, 596 603C, 610C , 617C, 624C, 631C, 638C, 645C, 652C, 659C, 666C, 673C 680C, 687C , 694C 687C 519, 526, 533, 540, 547,. 554, 610, 638, 645, 652, 659,. 715, 722 729, 733 344 756 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 215 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M5 (CONTINUED FROM PREVIOUS PAGE) C92814 T 105 NORMAL NO REMARKABLE OBSERVATIONS C92815 T MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 48 105 APPEARANCE MALOCCLUSION MISSING TEETH SKIN & PELAGE SORE/SCAB HEAD-MAXILLARY-LEFT NORMAL NO REMARKABLE OBSERVATIONS DOSE: 20 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 1, 8 15, 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 351, 358, 365 , 372, 379, 386, 393 , 400 407, 414, 421,. 428, 435, 442, 449, 456 , 463, 470, 477, 484 , 491 498, 505, 512, 561, 568, 575, 582, 589 , 596, 603, 617, 624 , 631 666, 673, 680, 687, 694, 701, 708 330, 337C 43, 50, 57, 64,r 71, 78, 85, 92, 99,r 106,r 113,r 1 2 0, 127,r 134, 14 148, 155 , 162, 169, 176, 183 , 190, 197, 204, 2 1 1 , 218, 225, 232 239, 246 , 253, 260, 267, 274 , 281, 288, 295, 302, 309, 316, 323 330, 337 , 344, 351, 358, 365 , 372, 379, 386, 393, 400, 407, 414 421, 428 , 435, 442, 449, 456 , 463, 470, 477, 484, 491, 498, 505 512, 519 , 526, 533, 540, 547 , 554, 561, 568, 575, 582, 589, 596 603, 610 , 617, 624, 631, 638 , 645, 652, 659, 666, 673, 680, 687 694, 701 , 708, 715, 722,. 729 , 732 610, 638 , 645, 652, 659, 666 , 673, 680, 687, 694, 701, 708, 715 722,. 729 , 732 64, 71, 78, 85,r 92, 99, 106, 113 1, 8 , 15, 22, 29, 36 757 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 216 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M5 DOSE: 20 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92816 D 64 NORMAL NO REMARKABLE OBSERVATIONS C92817 3 53 NORMAL NO REMARKABLE OBSERVATIONS C92818 T SURGERY PERFORMED BY PATTIE MURPHY ANTIBIOTIC ADMINISTERED 0.5 ML 105 APPEARANCE SWOLLEN PAW-HIND-RIGHT DISCHARGE GENITAL RED IN COLOR NORMAL NO REMARKABLE OBSERVATIONS 1 , 8 ,r 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190, 197 204, 2 1 1 , 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281, 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372, 379 386, 393, 400, 407 , 414, 421, 428, 435 , 442, 446 1 , 8 ,r 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190, 197 204, 2 1 1 , 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281, 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365- 366 359 359-360 680, 687, 694, 701, 708, 715, 722, 729, 732 176 1 , 8 , 15, 2 2 , 29, 36, 43,, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0, 127,, 134, 141, 148, 155,. 162, 169, 183, 190, 197, 204 758 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 217 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M5 (CONTINUED FROM PREVIOUS PAGE) C92818 T 105 NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 54 D 104 SKIN & PELAGE SORE/SCAB DORSAL-CERVICAL-LEFT DORSAL-THORACIC-LEFT PAW-HIND-LEFT VENTRAL-ABDOMINAL-LEFT VENTRAL-ABDOMINAL-RIGHT NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) DORSAL-CERVICAL-LEFT WEEK 25 DOSE: 20 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 2 1 1 , 218, 302, 309, 421, 428, 512, 519, 603, 610, 225, 316, 435, 526, 617, 232 , 239, 323 , 330, 442 , 449, 533 , 540, 624 , 631, 246, 337, 456, 547, 638, 253, 344, 463, 554, 645, 260 , 267, 351 , 358, 470 , 477, 561 , 568, 652 , 659, 274, 281, 365, 400, 484, 491, 575, 582, 666, 673 288 , 295 407 , 414 498 , 505 589 , 596 372, 379, 386, 393 267, 274, 358, 365, 449, 456, 239, 246, 666, 673, 281, 288, 141 281, 372, 463, 253, 680, 295 288 , 295, 379 , 386, 680 , 687, 260 687 , 694, 302, 393, 694, 701, 309, 400, 701, 708, 316 , 323, 407 , 414, 708 , 715, 715 , 722, 330, 421, 722, 727 337, 428, 727 344 , 351 435 , 442 1 , 8 ,r 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0, 127, 134 , 148, 155, 162 169, 176, 183, 190C, 197C, 204C, 211C, 218C, 225C, 232C, 239C 246C, 253C, 260C, 267C, 274C, 281C, 288C, 295C, 302C, 309C, 316C 323C, 330C, 337C, 344C, 351C, 358C, 365C, 372C, 379C, 386C, 393C 400C, 407C, 414C, 421C, 428C, 435C, 442C, 44 9C, 456C, 463C, 470C 477C, 484C, 491C, 498C, 505C, 512C, 519C, 526C, 533C, 54 0C, 547C 759 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 218 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M5 (CONTINUED FROM PREVIOUS PAGE) C92819 D 104 MASS(ES) DORSAL-CERVICAL-LEFT WEEK 25 VENTRAL-ABDOMINAL-LEFT WEEK 40 3 53 APPEARANCE MALOCCLUSION NORMAL NO REMARKABLE OBSERVATIONS DOSE: 20 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 554C , 561C, 568C, 575C, 582C, 589C , 596C, 603C, 610C, 617C, 624C 631C , 638C, 645C, 652C, 659C, 666C , 673C, 680C, 687C, 694C, 701C 708C , 715C, 722C, 727C 274C , 281C, 288C 71, 78, 85, 92, 99, 106, 113, 1 2 0, 127, 134, 141, 148,. 155, 162 169, 176, 183, 190, 197, 204, 2 1 1 , 218, 225, 232, 239,. 246, 253 260, 267, 274, 281, 288, 295, 302, 309, 316, 323, 330, 337, 344 351, 358, 365-366 1 , 8 , 15, 22, 29, 36, 43 , 50, 57, 64 760 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C92753 C92757 C92762 C92768 M5 624 90 MASS(ES) INGUINAL REGION-RIGHT WEEK 90 M5 680 98 MASS(ES) TO 731 105 DORSAL WEEK 98 M5 484 70 TO 498 72 MASS(ES) VENTRAL-ABDOMINAL WEEK 68 M5 596 86 MASS(ES) DORSAL WEEK 85 603 87 MASS(ES) DORSAL WEEK 85 610 88 MASS(ES) DORSAL WEEK 85 617 89 MASS(ES) DORSAL WEEK 85 624 90 MASS(ES) DORSAL WEEK 85 10 X 10MM 10 X 10MM 10 X 10MM 10 X 10MM 15 X 15MM 30 X 30MM 40 X 40MM 60 X 60MM 761 Covance 6329-183 3M T-6295 PAGE: 219 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 91 MASS(ES) DORSAL WEEK 85 92 MASS(ES) 94 DORSAL WEEK 85 95 MASS(ES) 95 DORSAL WEEK 85 77 MASS(ES) VENTRAL-ABDOMINAL WEEK 77 86 MASS(ES) 88 INGUINAL REGION-LEFT WEEK 86 86 MASS(ES) 88 INGUINAL REGION-RIGHT WEEK 86 89 MASS(ES) 90 VENTRAL-ABDOMINAL WEEK 89 75 MASS(ES) 80 DORSAL WEEK 72 80 X 70MM 70 X 60MM 80 X 100MM 10 X 10MM 10 X 10MM 10 X 10MM 20 X 20MM 10 X 10MM 762 Covance 6329-183 3M T-6295 PAGE: 220 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS CATEGORY KEYWORD WK QUALIFIER FREE-TEXT COMMENT 81 MASS(ES) 84 DORSAL WEEK 72 85 MASS(ES) 97 DORSAL WEEK 72 98 MASS(ES) 103 DORSAL WEEK 72 104 MASS(ES) 105 DORSAL WEEK 72 103 MASS(ES) 104 VENTRAL-ABDOMINAL-RIGHT WEEK 103 44 MASS(ES) 46 VENTRAL-ABDOMINAL-RIGHT WEEK 44 93 MASS(ES) 97 DORSAL WEEK 93 98 MASS(ES) 103 DORSAL WEEK 93 20 X 20MM 15 X 15MM 20 X 20MM 10 X 10MM 10 X 10MM 10 X 15MM 10 X 10MM 15 X 15MM 763 Covance 6329-183 3M T-6295 PAGE: 221 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS CATEGORY KEYWORD WK QUALIFIER FREE-TEXT COMMENT 104 MASS(ES) 105 DORSAL WEEK 93 101 MASS(ES) 102 AXILLARY REGION-LEFT WEEK 101 103 MASS(ES) 104 AXILLARY REGION-LEFT WEEK 101 105 MASS(ES) 105 AXILLARY REGION-LEFT WEEK 101 81 MASS(ES) 84 VENTRAL-ABDOMINAL-RIGHT WEEK 81 85 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 81 86 MASS(ES) 87 VENTRAL-ABDOMINAL-RIGHT WEEK 81 88 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 81 20 X 20MM 20 X 20MM 30 X 30MM 50 X 60 MM 40 X 40MM 40 X 50MM 60 X 60MM 70 X 60MM 764 Covance 6329-183 3M T-6295 PAGE: 222 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C92797 C92801 C92804 C92807 M5 617 89 624 90 631 91 TO 638 92 645 93 TO 653 94 M5 386 56 TO 393 57 365 53 TO 393 57 M5 386 56 TO 393 57 M5 267 39 TO 274 40 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 81 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 81 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 81 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 81 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 56 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 53 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 56 MASS(ES) VENTRAL-ABDOMINAL WEEK 39 80 X 70MM 100 X 80MM 110 X 100MM 120 X 110MM 70 X 80MM 15 X 15MM 10 X 10MM 10 X 10MM 765 Covance 6329-183 3M T-6295 PAGE: 223 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE- TEXT COMMENT C92807 C92808 C92809 M5 281 41 TO 288 42 MASS(ES) VENTRAL-ABDOMINAL WEEK 39 M5 729 105 MASS(ES) TO 731 105 AXILLARY REGION-LEFT WEEK 103 729 105 MASS(ES) TO 731 105 INGUINAL REGION-LEFT WEEK 98 M5 589 85 TO 603 87 MASS(ES) AXILLARY REGION-LEFT WEEK 85 610 88 TO 631 91 MASS(ES) AXILLARY REGION-LEFT WEEK 85 638 92 TO 659 95 MASS(ES) AXILLARY REGION-LEFT WEEK 85 666 96 TO 673 97 MASS(ES) AXILLARY REGION-LEFT WEEK 85 680 98 MASS(ES) TO 701 101 AXILLARY REGION-LEFT WEEK 85 15 X 15MM 10 X 10 MM 10 X 10 MM 25 X 20MM 30 X 30MM 30 X 40MM 35 X 40MM 50 X 45MM 766 Covance 6329-183 3M T-6295 PAGE: 224 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS CATEGORY KEYWORD WK QUALIFIER FREE-TEXT COMMENT 102 MASS(ES) AXILLARY REGION-LEFT WEEK 85 103 MASS(ES) AXILLARY REGION-LEFT WEEK 85 104 MASS(ES) AXILLARY REGION-LEFT WEEK 85 105 MASS(ES) 105 AXILLARY REGION-LEFT WEEK 85 86 MASS(ES) 87 INGUINAL REGION-LEFT WEEK 86 69 MASS(ES) 72 VENTRAL-ABDOMINAL-RIGHT WEEK 69 87 MASS(ES) 100 DORSAL WEEK 87 99 MASS(ES) DORSAL WEEK 99 50 X 50MM 60 X 70MM 70 X 60MM 70 X 80 MM 15 X 15MM 10 X 10MM 10 X 10MM 15 X 15MM 767 Covance 6329-183 3M T-6295 PAGE: 225 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 49 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 48 28 MASS(ES) 30 DORSAL-CERVICAL-LEFT WEEK 25 31 MASS(ES) 43 DORSAL-CERVICAL-LEFT WEEK 25 44 MASS(ES) 47 DORSAL-CERVICAL-LEFT WEEK 25 48 MASS(ES) 53 DORSAL-CERVICAL-LEFT WEEK 25 54 MASS(ES) 59 DORSAL-CERVICAL-LEFT WEEK 25 60 MASS(ES) 64 DORSAL-CERVICAL-LEFT WEEK 25 65 MASS(ES) DORSAL-CERVICAL-LEFT WEEK 25 10 X 10MM 10 X 10MM 20 X 20MM 25 X 25MM 25 X 20MM 30 X 20MM 30 X 30MM 40 X 30MM 768 Covance 6329-183 3M T-6295 PAGE: 226 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 66 MASS(ES) 68 DORSAL-CERVICAL-LEFT WEEK 25 69 MASS(ES) 73 DORSAL-CERVICAL-LEFT WEEK 25 74 MASS(ES) 77 DORSAL-CERVICAL-LEFT WEEK 25 78 MASS(ES) 84 DORSAL-CERVICAL-LEFT WEEK 25 85 MASS(ES) 88 DORSAL-CERVICAL-LEFT WEEK 25 89 MASS(ES) 90 DORSAL-CERVICAL-LEFT WEEK 25 91 MASS(ES) 92 DORSAL-CERVICAL-LEFT WEEK 25 93 MASS(ES) 96 DORSAL-CERVICAL-LEFT WEEK 25 30 X 30MM 40 X 30MM 50 X 40MM 50 X 50MM 70 X 60MM 60 X 50MM 75 X 55MM 80 X 55MM 769 Covance 6329-183 3M T-6295 PAGE: 227 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE- TEXT COMMENT C92819 M5 673 97 MASS(ES) TO 708 102 DORSAL-CERVICAL-LEFT WEEK 25 715 103 MASS(ES) TO 727 104 DORSAL-CERVICAL-LEFT WEEK 25 274 40 TO 288 42 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 40 80 X 60MM 60 X 50MM 10 X 10MM Covance 6329-183 3M T-6295 PAGE: 228 770 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 229 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M 6 DOSE: 20 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92821 U 106 APPEARANCE MALOCCLUSION MISSING TEETH SWOLLEN PAW-HIND-LEFT SKIN & PELAGE SORE/SCAB PAW-HIND-LEFT NORMAL NO REMARKABLE OBSERVATIONS 162, 253, 344, 435, 526, 617, 708, 169, 260, 351, 442, 533, 624, 715, 176, 267, 358, 449, 540, 631, 722, 183, 274, 365, 456, 547, 638, 729, 190, 281, 372, 463, 554, 645, 736 197, 288, 379, 470, 561, 652, 204, 295, 386, 477, 568, 659, 2 1 1 , 218, 302, 309, 393, 400, 484, 491, 575, 582, 666, 673, 225, 316, 407, 498, 589, 680, 232, 323, 414, 505, 596, 687, 239, 330, 421, 512, 603, 694, 246 337 428 519 610 701 344, 351, 358, 365, 372, 379, 386, 393, 400, 617, 624, 631, 638 645, 652, 659, 666, 673, 680, 687, 694, 701, 708, 715, 722, 729 736 729, 736 547, 554, 561, 568 , 575, 582, 589,. 596, 603, 610, 617,. 624, 631 638, 645, 652, 659 , 666, 673, 680,. 687, 694, 701, 708,. 715, 722 729, 736 1 , 8 ,r 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0, 127, 134 , 141, 148, 155 771 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 230 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M 6 DOSE: 20 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92822 M 81 APPEARANCE HEAD TILT LEFT THIN BEHAVIOR ATAXIC <UNSPECIFIED> HYPOACTIVE <UNSPECIFIED> EXCRETION FEW FECES SKIN & PELAGE SORE/SCAB VENTRAL-ABDOMINAL NORMAL NO REMARKABLE OBSERVATIONS SACRIFICE SACRIFICE APPROVED BY CERTIFIED TECHNICIAN 566 554, 561, 566 561, 566 554, 561, 566 566 456, 463, 470 1 , 8 ,r 15, 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 477, 484 , 491 498, 505, 512, 519, 526, 533, 540, 547 566 772 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 231 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M 6 DOSE: 20 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92823 U 106 APPEARANCE MALOCCLUSION C92824 D lM/ IITSO SC ITTvNT/G~< TEETH SKIN & PELAGE SORE/SCAB VENTRAL-ABDOMINAL NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) VENTRAL-ABDOMINAL WEEK 74 95 APPEARANCE THIN EXCRETION FEW FECES LIQUID FECES NO FECES 36, 43, 50, 57,r 64, 141, 148 , 155, 162, 232, 239 , 246, 253, 323, 330 , 337, 344, 414, 421 , 428, 435, 505, 512 , 519, 526, 596, 603 , 610, 617, 687,. 694 , 701, 708, 71, 78, 85, 92, 99, 169, 176 , 183, 190, 260, 267 , 274, 281, 351, 358 , 365, 372, 442, 449 , 456, 463, 533, 540 , 547, 554, 624, 631 , 638, 645, 715, 722 , 729, 736 106, 197, 288, 379, 470, 561, 652, 113, 204, 295, 386, 477, 568, 659, 1 2 0, 127, 2 1 1 , 218, 302, 309, 393, 400, 484, 491, 575, 582, 666, 673, 134 225 316 407 498 589 680 736 435, 442, 533 1, 8 , 15, 22, 29 512C, 519C, 526C 535 535 535 582 773 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 232 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M 6 (CONTINUED FROM PREVIOUS PAGE) C92824 D 95 RESPIRATION AUDIBLE SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE SORE/SCAB PAW-HIND-LEFT PAW-HIND-RIGHT PAWS-HIND NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) AXILLARY REGION-RIGHT WEEK 66 C92825 D 94 APPEARANCE SWOLLEN LIMB-HIND-LEFT PAW-HIND-LEFT SKIN & PELAGE SORE/SCAB VENTRAL-ABDOMINAL-RIGHT DOSE: 20 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 582, 589, 596 535 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379, 386 , 393 400, 407 456, 463 470, 477, 484, 491, 498, 505 1 , 8 ,. 15, 2 2 , 29, 36, 43 , 50, 57, 64, 71, 78,, 85, 92, 99, 106 113, 1 2 0, 127,. 134, 141, 148, 155, 162, 169, 176, 183, 190 , 197 204, 2 1 1 , 218,. 225, 232, 239, 246, 253, 260, 267, 274, 281 , 288 295, 302, 414,. 421, 428, 435, 442, 449 456C, 463C , 470C, 477C, 484C, 491C , 498C, 505C, 512C, 519C , 526C 533C, 535C , 540C, 547C, 554C, 561C , 568C, 575C, 582C, 589C , 596C 603C, 610C , 617C, 624C, 631C, 638C , 645C, 652C, 659C, 663C 428, 435, 442,. 449, 456, 463 428, 435, 442,. 449, 456, 463 540, 547, 554 774 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 233 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M 6 (CONTINUED FROM PREVIOUS PAGE) C92825 D 94 NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 75 D 55 DISCHARGE CLEAR ORAL <UNSPECIFIED> RESPIRATION AUDIBLE NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 45 DOSE: 20 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 1 , 8 , 15, 2 2 , 29, 36, 43 , 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218,. 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400,. 407, 414, 421, 470, 477 , 484, 491, 498, 505 , 512 561, 568, 575,. 582, 589, 596, 603, 610 , 617, 624, 631, 638 , 645 652- 653 519C , 526C', 53 3C, 54 0C, 547C 385 385 1 , 8 , 15, 2 2 , 29, 36, 43 , 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218,. 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302 309, 316, 323, 330, 337, 344, 351, 358 , 365, 372, 379, 385 775 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 234 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M 6 DOSE: 20 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92827 U 106 SKIN & PELAGE SORE/SCAB AXILLARY REGION-RIGHT DORSAL NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) DORSAL WEEK 92 VENTRAL-ABDOMINAL-RIGHT WEEK 97 WART LIKE LESION(S) AXILLARY REGION-RIGHT WEEK 87 645, 652, 659, 666, 673, 680, 687, 694, 701, 708, 715, 722 638, 645, 652, 659, 666, 673, 680, 687, 694, 701, 708, 715, 722 729, 736 1 , 8 ,r 15, 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582, 589, 596 638C, 645C, 652C, 659C, 666C, 673C, 680C, 687C, 694C, 701C, 708' 715C, 722C, 729C, 736C 673C, 680C, 687C, 694C, 701C, 708C 603, 610, 617, 624, 631, 638, 645, 652, 659, 666, 673, 680, 687 694, 701, 708, 715 776 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 235 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M 6 DOSE: 20 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92828 D 83 APPEARANCE MALOCCLUSION SWOLLEN TESTIS-LEFT DISCHARGE NASAL RED IN COLOR NORMAL NO REMARKABLE OBSERVATIONS C92829 U 106 APPEARANCE SWOLLEN PAW-HIND-LEFT PAW-HIND-RIGHT SKIN & PELAGE SORE/SCAB VENTRAL-ABDOMINAL NORMAL NO REMARKABLE OBSERVATIONS 470, 512 533, 540, 547,. 554, 561, 568, 575, 578 197 1 , 8 ,r 15, 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 204 2 1 1 , 218, 225,. 232, 239, 246, 253, 260 , 267, 274, 281, 288 , 295 302, 309, 316,. 323, 330, 337, 344, 351 , 358, 365, 372, 379 , 386 393, 400, 407,. 414, 421, 428, 435, 442 , 449, 456, 463, 477 , 484 491, 498, 505, 519, 526 603, 610, 617,. 624, 631, 638, 645, 652 , 659, 666, 673 575, 582, 589,. 596, 603, 610, 617, 624 , 631, 638, 645, 652 , 659 666, 673, 680, 687, 694, 701, 708, 715 , 722, 729, 736 736 1 , 8 ,r 15, 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253 , 260, 267, 274, 302 , 309 316, 323, 330, 337, 344, 351, 358, 365 , 372, 379, 386, 393 , 400 777 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 236 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M 6 (CONTINUED FROM PREVIOUS PAGE) C92829 U 106 NORMAL NO REMARKABLE OBSERVATIONS C92830 M MASS(ES) VENTRAL-ABDOMINAL WEEK 41 WART LIKE LESION(S) TAIL WEEK 61 99 APPEARANCE MALOCCLUSION THIN BEHAVIOR COLD TO TOUCH ENTIRE BODY EXCRETION FEW FECES RESPIRATION IRREGULAR DOSE: 20 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 407, 414, 442, 449, 456, 463, 470, 477, 484, 491, 498, 505, 512 519, 526, 533, 540, 547, 554, 561, 568 281C, 288C, 295C 421, 428, 435 134, 141, 225, 232, 316, 323, 407, 414, 498, 505, 589, 596, 680, 687, 666, 673, 148, 239, 330, 421, 512, 603, 690 680, 155, 246, 337, 428, 519, 610, 687, 162, 253, 344, 435, 526, 617, 690 169, 260, 351, 442, 533, 624, 176, 267, 358, 449, 540, 631, 183, 274, 365, 456, 547, 638, 190, 281, 372, 463, 554, 645, 197, 288, 379, 470, 561, 652, 204, 295, 386, 477, 568, 659, 2 1 1 , 218 302, 309 393, 400 484, 491 575, 582 666, 673 687, 690 687, 690 778 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 237 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER (CONTINUED FROM PREVIOUS PAGE) C92830 M 99 SKIN & PELAGE SORE/SCAB EAR-RIGHT GROUP: M 6 OTHER NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) EAR-RIGHT WEEK 47 C92831 M WART LIKE LESION(S) EAR-RIGHT WEEK 41 85 APPEARANCE LIMITED USE LIMBS-ALL LIMBS-HIND MALOCCLUSION DOSE: 20 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 358, 449, 540, 631, 690C 365, 456, 547, 638, 372, 463, 554, 645, 379 , 386, 470 , 477, 561 , 568, 652 , 659, 393, 400, 484, 491, 575, 582, 666, 673, 407, 498, 589, 680, 414, 5 05, 5 96, 687, 421, 512, 603, 690 428 , 435 , 442 519 , 526 , 533 610 , 617 , 624 1 , 8 ,. 15, 2 2 , 29, 36, 43 , 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0, 127 323, 330, 337, 344 , 351, 358, 365, 372C, 379C, 386C, 393C, 400C 407C, 414C , 421C, 428C, 435C, 442C , 449C, 456C, 463C, 470C , 477C 484C, 491C , 498C, 505C, 512C, 519C , 526C, 533C, 540C, 547C , 554C 561C, 568C , 575C, 582C, 58 9C, 596C , 603C, 610C, 617C, 624C , 631C 638C, 645C , 652C, 659C, 666C, 673C , 680C, 687C, 690C 281,288 585, 589 582 2 1 1 , 218 302, 309 393, 400 484, 491 225, 232, 239, 246, 253, 260, 267, 274, 281, 288, 295 316, 323, 330, 337, 344, 351, 358, 365, 372, 379, 386 407, 414, 421, 428, 435, 442, 449, 456, 463, 470, 477 498, 505, 512, 519, 526, 533, 540, 547, 554, 561, 568 779 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 238 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M6 (CONTINUED FROM PREVIOUS PAGE) C92831 M 85 APPEARANCE MALOCCLUSION SWOLLEN NOSE BEHAVIOR HYPOACTIVE <UNSPECIFIED> RECUMBENT STERNAL COLD TO TOUCH ENTIRE BODY EYES RED DISCHARGE EYE-RIGHT SKIN & PELAGE SORE/SCAB MOUTH NORMAL NO REMARKABLE OBSERVATIONS DOSE: 20 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 575, 582, 585, 589 92 589 589 589 92, 589 92 1 22, 8,, 15, , 29, 36, 43,, 50, 57, 64, 71, 78, 85, 99, 106, 113 120 20, 127, 134,, 141, 148, 155, 162,, 169, 176, 183, 190, 197, 780 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 239 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M 6 DOSE: 20 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92832 D 69 NORMAL NO REMARKABLE OBSERVATIONS U 106 APPEARANCE OBES SWOLLEN LIMB-HIND-RIGHT PAW-HIND-RIGHT SKIN & PELAGE RED SKIN PAW-HIND-RIGHT NORMAL NO REMARKABLE OBSERVATIONS 1 , 8 ,r 15, 2 2 , 29, 36 , 43,r 50, 113, 1 2 0 , 127,. 134, 141, 148, 204, 2 1 1 , 218, 225, 232, 239, 295, 302, 309,. 316, 323, 330, 386, 393, 400, 407, 414, 421, 477, 480 57, 64, 71, 78, 85, 155, 162 , 169, 176, 246, 253 , 260, 267, 337, 344 , 351, 358, 428, 435 , 442, 449, 92, 99, 106 183, 190 , 197 274, 281 , 288 365, 372 , 379 456, 463 , 470 477 622, 624, 631,. 638, 645, 652, 659, 666 , 673, 680, 687, 694 , 701 708, 715, 722,. 729, 736 680, 687, 694, 701, 708, 715, 722, 729 , 736 622 1 , 8 ,r 15, 2 2 , 29, 36 , 43,r 50, 113, 1 2 0 , 127 134, 141, 148, 204, 2 1 1 , 218 225, 232, 239, 295, 302, 309 316, 323, 330, 386, 393, 400 407, 414, 421, 484, 491, 498 505, 512, 519, 575, 582, 589 596, 603, 610, 57, 64, 71, 78, 85, 155, 162 , 169, 176, 246, 253 , 260, 267, 337, 344 , 351, 358, 428, 435 , 442, 449, 526, 533 , 540, 547, 617 92, 99, 106 183, 190 , 197 274, 281 , 288 365, 372 , 379 456, 463 , 470 554, 561 , 568 781 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 240 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M 6 DOSE: 20 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92834 U 106 SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE SORE/SCAB SHOULDER-RIGHT NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) SHOULDER-RIGHT WEEK 55 C92835 M WART LIKE LESION(S) SHOULDER-RIGHT WEEK 95 75 BEHAVIOR HYPOACTIVE <UNSPECIFIED> RECUMBENT DORSAL COLD TO TOUCH ENTIRE BODY 379 407, 414, 421,. 428, 435, 442, 449, 456, 463, 470 , 477, 484, 491 498, 505, 512,. 519, 526, 533, 540, 547, 554, 561 , 568, 575, 582 589, 596, 603,. 610, 617, 624, 631, 638, 645, 652 1, 8 , 15, 2 2 , 29, 36, 43 , 50, 57, 64, 71, 78 , 85 , 92, 99, 106 113, 1 2 0 , 127,. 134, 141, 148, 155, 162, 169, 176 , 183, 190, 197 204, 2 1 1 , 218,. 225, 232, 239, 246, 253, 260, 267 , 274, 281, 288 295, 302, 309,. 316, 323, 330, 337, 344, 351, 358 , 365, 372 379, 386, 393, 400, 407C , 414C , 421C, 428C, 435C , 442C , 449C 456C, 463C , 470C, 477C, 484C, 491C , 498C, 505C, 512C, 519C, 526C 533C, 540C', 54 7C, 554C, 561C, 568C , 575C, 582C 659, 666, 673, 680, 687, 694, 701, 708, 715, 722, 729, 736 522 522 522 782 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 241 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M 6 (CONTINUED FROM PREVIOUS PAGE) C92835 M 75 EXCRETION FEW FECES EYES RED DISCHARGE EYE-RIGHT EYES RESPIRATION LABORED NORMAL NO REMARKABLE OBSERVATIONS D 91 BEHAVIOR HYPOACTIVE <UNSPECIFIED> RESPIRATION LABORED SKIN & PELAGE PALE ENTIRE BODY NORMAL NO REMARKABLE OBSERVATIONS DOSE: 20 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 522 246, 281 522 522 1 , 8 ,r 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225 , 232, 239, 253, 260 , 267, 274, 288, 295 , 302 309, 316, 323, 330 , 337, 344, 351, 358 , 365, 372, 379, 386 , 393 400, 407, 414, 421 , 428, 435, 442, 449 , 456, 463, 470, 477 , 484 491, 498, 505, 512 , 519 1 , 8 , 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92 783 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 242 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M 6 (CONTINUED FROM PREVIOUS PAGE) C92836 D 91 NORMAL NO REMARKABLE OBSERVATIONS C92837 M 65 APPEARANCE THIN EXCRETION FEW FECES SKIN & PELAGE ROUGH HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS C92838 M 65 EXCRETION FEW FECES NONFORMED FECES SKIN & PELAGE BROWN HAIR COAT SCROTUM DOSE: 20 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 113, 204, 295, 386, 477, 568, 12 0 , 127, 2 1 1 , 218, 302, 309, 393, 400, 484, 491, 575, 582, 134, 225, 316, 407, 498, 589, 141, 232, 323, 414, 505, 596, 148, 239, 330, 421, 512, 603, 155, 246, 337, 428, 519, 610, 162, 253, 344, 435, 526, 617, 169, 260, 351, 442, 533, 624 176, 267, 358, 449, 540, 183, 274, 365, 456, 547, 190, 281, 372, 463, 554, 197 288 379 470 561 449 445, 449 445, 449 1, 8 , 15 113, 120 204, 211 295, 302 386, 393 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 400, 407 , 414, 421, 428, 435 , 442 449-450 450 450 784 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 243 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M 6 (CONTINUED FROM PREVIOUS PAGE) C92838 M 65 SKIN & PELAGE SORE/SCAB DORSAL NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) DORSAL WEEK 53 WART LIKE LESION(S) DORSAL WEEK 64 D 89 APPEARANCE OBES EYES RED DISCHARGE EYE-LEFT NORMAL NO REMARKABLE OBSERVATIONS DOSE: 20 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 365, 372, 379, 386 1 , 8 ,r 15, 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 393, 400 , 407 414, 421, 428, 435 365, 372, 379 442, 449- 450 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582, 589, 596, 603, 610 575, 617, 623 1 , 8 ,r 15, 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 785 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 244 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M 6 (CONTINUED FROM PREVIOUS PAGE) C92839 D 89 NORMAL NO REMARKABLE OBSERVATIONS C92840 M 103 APPEARANCE THIN DISCHARGE NASAL RED IN COLOR EXCRETION FEW FECES SKIN & PELAGE BROWN HAIR COAT VENTRAL-ABDOMINAL NORMAL NO REMARKABLE OBSERVATIONS DOSE: 20 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470 715 708 701, 708, 715 715 1 , 8 , 15 113, 120 204, 211 295, 302 386, 393 477, 484 568, 575 659, 666 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 582, 589, 596, 603, 610, 617 , 624, 631, 638, 645 , 652 673, 680, 687, 694 786 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 245 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M 6 DOSE: 20 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92841 D 68 NORMAL NO REMARKABLE OBSERVATIONS M 91 APPEARANCE CONVULSIONS BEHAVIOR HYPOACTIVE <UNSPECIFIED> SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) HEAD-MAXILLARY-LEFT WEEK 88 1 , 8 ,r 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407 , 414, 421, 428, 435 , 442, 449, 456, 463 , 470 476 633 633 633 1 , 8 ,r 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407 , 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498 , 505, 512, 519, 526 610, 617, 624 787 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 246 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M 6 (CONTINUED FROM PREVIOUS PAGE) C92842 M 91 WART LIKE LESION(S) DORSAL WEEK 77 C92843 D 77 NORMAL NO REMARKABLE OBSERVATIONS C92844 M 83 APPEARANCE MALOCCLUSION THIN BEHAVIOR HYPOACTIVE <UNSPECIFIED> EXCRETION FEW FECES EYES RED DISCHARGE EYE-RIGHT NORMAL NO REMARKABLE OBSERVATIONS DOSE: 20 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 533, 540, 547, 554, 561, 568, 575, 582, 589, 596, 603, 610, 617 624, 631, 633 1 , 8 ,r 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407 , 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498 , 505, 512, 519, 526 , 533, 537 295, 428, 435, 442 , 463, 470, 477 579 579 579 579 1 , 8 , 15, 22, 29, 36, 43,, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127, 134, 141, 148, 155, 162 , 169, 176, 183,. 190, 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281, 288 788 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 247 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M 6 (CONTINUED FROM PREVIOUS PAGE) C92844 M 83 NORMAL NO REMARKABLE OBSERVATIONS C92845 M 95 APPEARANCE HEAD TILT RIGHT BEHAVIOR RECUMBENT LATERAL EXCRETION FEW FECES NO FECES RESPIRATION IRREGULAR NORMAL NO REMARKABLE OBSERVATIONS DOSE: 20 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379, 386 393, 400, 407, 414, 421, 449, 456, 484, 491, 498, 505, 512, 519 526, 533, 540, 547, 554, 561, 568, 575 663-664 664 663 664 1, 8 , 15 113, 120 204, 211 295, 302 386, 393 477, 484 568, 575 659 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 582, 589, 596, 603, 610, 617 , 624, 631, 638, 645 , 652 789 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 248 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M 6 DOSE: 20 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92846 D 103 APPEARANCE MALOCCLUSION EXCRETION FEW FECES SKIN & PELAGE SORE/SCAB DORSAL-THORACIC-RIGHT VENTRAL-ABDOMINAL VENTRAL-ABDOMINAL-LEFT NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) DORSAL WEEK 55 DORSAL-CERVICAL-RIGHT WEEK 69 INGUINAL REGION-LEFT WEEK 91 99, 106, 113, 1 2 0 , 127, 134, 141, 148, 155, 162, 169, 176, 183 190, 197, 204, 2 1 1 , 218, 225, 232, 239, 246, 253, 260, 267, 274 281, 288, 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365 372, 379, 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456 463, 470, 477, 484, 491, 498, 505, 512, 519, 526, 533, 540, 547 554, 561, 568, 575, 582, 589, 596, 603, 610, 617, 624, 631, 638 645, 652, 659, 666, 673, 680, 687, 694, 701, 708, 715, 721 603 456, 547 393 659, 463, 470, 666, 673 477, 484, 491, 498, 505, 512, 519, 526, 533,, 540 1 , 8 ,. 15, 22, 29, 36, 43, 50,. 57, 64, 71 , 78, 85, 92 379, 386C, 393C, 400C, 407C, 414C, 421C, 428C, 435C, 442C, 449C 477C,. 484C, 491C, 498C, 505C, 512C , 519C , 526C, 533C 631C, 638C, 645C, 652C 790 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 249 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M 6 (CONTINUED FROM PREVIOUS PAGE) C92846 D 103 WART LIKE LESION(S) DORSAL WEEK 80 C92847 M 98 APPEARANCE THIN EXCRETION FEW FECES EYES RED DISCHARGE EYE-LEFT SKIN & PELAGE SORE/SCAB PAW-HIND-RIGHT NORMAL NO REMARKABLE OBSERVATIONS DOSE: 20 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 554, 561, 568, 575, 582, 589, 596, 603, 610, 617, 624, 631, 638 645, 652, 659, 666, 673, 680, 687, 694, 701, 708, 715, 721 680 673, 680 680 547, 554, 561,. 568, 575, 582, 589, 596 , 603, 610, 617, 624 , 631 638, 645, 652, 659, 666, 673, 680 1 , 8 ,r 15, 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540 791 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 250 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M 6 DOSE: 20 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92848 U 106 APPEARANCE LIMITED USE PAW-HIND-LEFT SWOLLEN LIMB-HIND-LEFT PAW-HIND-LEFT THIN NORMAL NO REMARKABLE OBSERVATIONS C92849 M 70 APPEARANCE MALOCCLUSION BEHAVIOR HYPOACTIVE MODERATE COLD TO TOUCH LIMBS-ALL ENTIRE BODY EXCRETION FEW FECES NO FECES 617, 624, 631, 638 , 645, 652, 659, 666 , 673, 680, 687, 694 , 701 708, 715, 722,. 729 , 736 585, 589, 596, 603 , 610 585, 589, 596, 603 , 610, 617, 624, 631 , 638, 645, 652, 659 , 666 673, 680, 687,. 694 , 701, 708, 715, 722 , 729, 736 729, 736 1 , 8 ,r 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407 , 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498 , 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582 484 484 470, 477, 484 484 470, 477, 484 484 792 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 251 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M6 (CONTINUED FROM PREVIOUS PAGE) C92849 M 70 EYES SQUINTED PERI-ORBITAL NORMAL NO REMARKABLE OBSERVATIONS C92850 M 105 APPEARANCE MALOCCLUSION SWOLLEN LIMB-HIND-RIGHT PAW-HIND-RIGHT THIN BEHAVIOR HYPOACTIVE <UNSPECIFIED> RECUMBENT LATERAL EXCRETION NONFORMED FECES SKIN & PELAGE PALE ENTIRE BODY DOSE: 20 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 477, 484 1 22, 8,r 15, , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 120113, , 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 211204, , 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407 , 414, 421, 428, 435 , 442, 449, 456, 463 379, 386, 393, 400, 407, 414, 421, 428, 435, 442 659, 666, 673 659, 666, 673 722, 729- 730 730 730 344 722, 729-730 793 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 252 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M 6 (CONTINUED FROM PREVIOUS PAGE) C92850 M 105 NORMAL NO REMARKABLE OBSERVATIONS U 106 SKIN & PELAGE SORE/SCAB VENTRAL-ABDOMINAL NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 69 WART LIKE LESION(S) VENTRAL-ABDOMINAL WEEK 102 DOSE: 20 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 1 , 8 ,r 15, 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 351 , 358, 365, 372, 449 , 456 463, 470, 477,. 484, 491, 498, 505, 512 , 519, 526, 533, 540 , 547 554, 561, 568,. 575, 582, 589, 596, 603 , 610, 617, 624, 631 , 638 645, 652, 680, 687, 694, 701, 708, 715 603, 610, 617,. 624, 631, 638, 645, 652 , 659, 666, 673, 680 , 687 694, 701, 708 1 , 8 ,r 15, 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 484, 491, 498, 505, 512, 519, 526, 533 , 540, 547, 554, 561 , 568 575, 582, 589, 596 477 708, 715, 722, 729, 736 794 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 253 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M 6 DOSE: 20 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92852 M 68 APPEARANCE HUNCHED THIN BEHAVIOR HYPOACTIVE <UNSPECIFIED> EXCRETION FEW FECES RESPIRATION IRREGULAR NORMAL NO REMARKABLE OBSERVATIONS U 106 NORMAL NO REMARKABLE OBSERVATIONS 475 474-475 475 474-475 475 1 , 8 ,r 15, 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 1 , 8 ,r 15, 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582, 589, 596, 603, 610, 617 , 624, 631, 638, 645 , 652 659, 666, 673, 680, 687, 694, 701, 708 , 715, 722, 729, 736 795 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 254 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M 6 DOSE: 20 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92854 D 101 EXCRETION NONFORMED FECES SKIN & PELAGE SORE/SCAB VENTRAL-ABDOMINAL-LEFT NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 84 WEEK 87 WART LIKE LESION(S) VENTRAL-ABDOMINAL-RIGHT WEEK 99 113 617, 624 631 1 , 8 , 15 1 2 0, 127 2 1 1 , 218 302, 309 393, 400 484, 491 575, 596 2 2 , 29, 36 , 43,r 50, 134, 141, 148, 155, 225,. 232, 239, 246, 316,. 323, 330, 337, 407,. 414, 421, 428, 498, 505, 512, 519, 638, 645, 652, 659, 57, 64, 71, 78, 85, 162, 169 , 176, 183, 253, 260 , 267, 274, 344, 351 , 358, 365, 435, 442 , 449, 456, 526, 533 , 540, 547, 666, 673 , 680, 694, 92, 99, 106 190, 197 , 204 281, 288 , 295 372, 379 , 386 463, 470 , 477 554, 561 , 568 701, 705 582, 589 603, 610 687 796 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 255 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M 6 DOSE: 20 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92855 M 70 APPEARANCE HUNCHED MALOCCLUSION BEHAVIOR HYPOACTIVE <UNSPECIFIED> DISCHARGE GENITAL RED IN COLOR SKIN & PELAGE ROUGH HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS WART LIKE LESION(S) VENTRAL-ABDOMINAL-LEFT WEEK 69 484 50, 57, 64, 71,, 78, 85, 92, 99, 106 , 113 , 120 , 127 , 134 , 141 148, 155 , 162, 169, 176, 183 , 190, 197, 204, 2 1 1 , 218, 225, 232 239, 246 , 253, 260, 267, 274 , 281, 288, 295, 302, 309, 316, 323 330, 337 , 344, 351, 358, 365 , 372, 379, 386, 393, 400, 407, 414 421, 428 , 435, 442, 449, 456 , 463, 470, 477, 484 484 176, 183 463, 470, 477, 484 1, 8 , 15, 22, 29, 36, 43 477, 484 797 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 256 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M 6 DOSE: 20 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92856 U 106 APPEARANCE BENT TAIL C92857 M SWOLLEN PAW-HIND-RIGHT NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) INGUINAL REGION-LEFT WEEK 99 83 APPEARANCE SWOLLEN PAW-HIND-LEFT BEHAVIOR HYPOACTIVE <UNSPECIFIED> RECUMBENT STERNAL EXCRETION FEW FECES NO FECES 43, 50, 57, 64 , 71 , 78, 85, 92, 99 , 106 , 113 , 120 , 127 , 134 , 141 148, 155 , 162, 169 , 176, 183 , 190, 197, 204, 2 1 1 , 218, 225, 232 239, 246 , 253, 260 , 267, 274 , 281, 288, 295, 302, 309, 316, 323 330, 337 , 344, 351 , 358, 365 , 372, 379, 386, 393, 400, 407, 414 421, 428 , 435, 442 , 449, 456 , 463, 470, 477, 484, 491, 498, 505 512, 519 , 526, 533 , 540, 547 , 554, 561, 568, 575, 582, 589 595- 596, 603, 610, 617, 624, 631, 638, 645, 652, 659, 666, 673 680, 687 , 694, 701 , 708, 715 , 722, 729, 736 595- 596, 603, 610 1 , 8 , 15 , 2 2 , 29, 36 687, 694, 701, 708, 715, 722, 729, 736 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470 576 576 575 576 798 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 257 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M 6 (CONTINUED FROM PREVIOUS PAGE) C92857 M 83 EYES RED DISCHARGE EYES SQUINTED PERI-ORBITAL NORMAL NO REMARKABLE OBSERVATIONS C92858 M 97 APPEARANCE THIN EXCRETION FEW FECES RESPIRATION AUDIBLE NORMAL NO REMARKABLE OBSERVATIONS DOSE: 20 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 576 576 1, 8 , 15 113, 120 204, 211 295, 302 386, 393 554, 561 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 477, 484 , 491, 498, 505, 512 , 519, 526, 533, 540 , 547 568 673 659, 666, 673 673 1 , 8 ,r 15, 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0, 127 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 540, 547, 554 561, 568, 575, 582, 589 , 596 799 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 258 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M 6 (CONTINUED FROM PREVIOUS PAGE) C92858 M 97 MASS(ES) AXILLARY REGION-RIGHT WEEK 91 INGUINAL REGION-LEFT WEEK 69 WART LIKE LESION(S) DORSAL WEEK 76 WEEK 87 C92859 M 72 APPEARANCE HEAD TILT RIGHT EXCRETION NONFORMED FECES SKIN & PELAGE ROUGH HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS DOSE: 20 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 631C, 638C, 64 5C, 652C, 659C, 666C, 673C 477, 484, 491, 498, 505, 512, 519, 526, 533 526, 533 603, 610, 617, 624, 631, 638, 645, 652, 659, 666, 673 498, 500 393 491, 498, 500 1 , 8 ,r 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 400, 407, 414 , 421, 428, 435, 442 , 449, 456, 463, 470 , 477 484 800 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 259 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M 6 DOSE: 20 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92860 M 97 APPEARANCE LIMITED USE LIMBS-HIND BEHAVIOR ATAXIC <UNSPECIFIED> EXCRETION FEW FECES EYES RED DISCHARGE EYES SKIN & PELAGE ROUGH HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS WART LIKE LESION(S) VENTRAL-THORACIC-LEFT WEEK 96 673, 678 678 673, 678 678 678 1, 8 , 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 659 2 2 , 29, 36 , 43,r 50, 127,. 134, 141, 148, 218, 225, 232, 239, 309,. 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 57, 64, 71, 78, 85, 155, 162 , 169, 176, 246, 253 , 260, 267, 337, 344 , 351, 358, 428, 435 , 442, 449, 519, 526 , 533, 540, 610, 617 , 624, 631, 92, 99, 106 183, 190 , 197 274, 281 , 288 365, 372 , 379 456, 463 , 470 547, 554 , 561 638, 645 , 652 666, 673, 678 801 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 74 MASS(ES) 76 VENTRAL-ABDOMINAL WEEK 74 66 MASS(ES) AXILLARY REGION-RIGHT WEEK 66 67 MASS(ES) 68 AXILLARY REGION-RIGHT WEEK 66 69 MASS(ES) 71 AXILLARY REGION-RIGHT WEEK 66 72 MASS(ES) 75 AXILLARY REGION-RIGHT WEEK 66 76 MASS(ES) 77 AXILLARY REGION-RIGHT WEEK 66 78 MASS(ES) 79 AXILLARY REGION-RIGHT WEEK 66 80 MASS(ES) 84 AXILLARY REGION-RIGHT WEEK 66 15 X 15MM 15 X 15MM 25 X 15MM 30 X 25MM 35 X 30MM 40 X 35MM 50 X 50MM 45 X 40MM 802 Covance 6329-183 3M T-6295 PAGE: 260 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS STUDY DAY WK CATEGORY KEYWORD QUALIFIER FREE- TEXT C92824 M6 C92825 M6 C92827 M6 589 85 624 90 MASS(ES) AXILLARY REGION-RIGHT WEEK 66 631 91 652 94 MASS(ES) AXILLARY REGION-RIGHT WEEK 66 659 95 663 95 MASS(ES) AXILLARY REGION-RIGHT WEEK 66 519 75 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 75 526 76 547 79 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 75 638 92 673 97 MASS(ES) DORSAL WEEK 92 680 98 MASS(ES) 715 103 DORSAL WEEK 92 722 104 MASS(ES) 736 106 DORSAL WEEK 92 45 X 60MM 50 X 60MM 50 X 70MM 10 X 10MM 15 X 15MM 10 X 10MM 15 X 10MM 10 X 10MM 803 Covance 6329-183 3M T-6295 PAGE: 261 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 97 MASS(ES) 10. VENTRAL-ABDOMINAL-RIGHT WEEK 97 41 MASS(ES) VENTRAL-ABDOMINAL WEEK 41 42 MASS(ES) 43 VENTRAL-ABDOMINAL WEEK 41 99 SKIN & PELAGE OTHER 54 MASS(ES) 55 EAR-RIGHT WEEK 47 56 MASS(ES) 88 EAR-RIGHT WEEK 47 89 MASS(ES) 95 EAR-RIGHT WEEK 47 96 MASS(ES) 99 EAR-RIGHT WEEK 47 10 X 10MM 20 X 20MM 10 X 10MM COLD TO TOUCH 10 X 10MM 10 X 15MM 20 X 20MM 30 X 20MM 804 Covance 6329-183 3M T-6295 PAGE: 262 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 59 MASS(ES) 71 SHOULDER-RIGHT WEEK 55 72 MASS(ES) 82 SHOULDER-RIGHT WEEK 55 83 MASS(ES) 84 SHOULDER-RIGHT WEEK 55 56 MASS(ES) 65 DORSAL WEEK 55 69 MASS(ES) 73 DORSAL-CERVICAL-RIGHT WEEK 69 74 MASS(ES) 77 DORSAL-CERVICAL-RIGHT WEEK 69 91 MASS(ES) 92 INGUINAL REGION-LEFT WEEK 91 93 MASS(ES) 94 INGUINAL REGION-LEFT WEEK 91 10 X 10MM 15 X 10MM 20 X 10MM 15 X 10MM 10 X 10MM 15 X 15MM 15 X 15MM 20 X 20MM 805 Covance 6329-183 3M T-6295 PAGE: 263 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER M6 631 91 TO 645 93 652 94 TO 666 96 673 97 TO 673 97 MASS(ES) AXILLARY REGION-RIGHT WEEK 91 MASS(ES) AXILLARY REGION-RIGHT WEEK 91 MASS(ES) AXILLARY REGION-RIGHT WEEK 91 FREE-TEXT COMMENT 10 X 10MM 15 X 15MM 15 X 20MM Covance 6329-183 3M T-6295 PAGE: 264 806 COVAH^ THE DEVELOPMENT SERVICES COMPANY Sponsor: 3M St. Paul, Minnesota FINAL REPORT Study Title: 104-Week Dietary Chronic Toxicity and Carcinogenicity Study with Perfluorooctane Sulfonic Acid Potassium Salt (PFOS; T-6295) in Rats Author: Peter J. Thomford, PhD Study Completion Date: January 2, 2002 Testing Facility: Covance Laboratories Inc. 3301 Kinsman Boulevard Madison, Wisconsin 53704-2595 Laboratory Study Identification: Covance 6329-183 Sponsor Study Identification: 3M T-6295 Volume III of IX Page 807 of 4068 Covance 6329-183 3M T-6295 VOLUME III OF IX CONTENTS Page APPENDIX 2 (Continued)..............................................................................................809 Individual Clinical Observations................................................................................. 810 808 APPENDIX 2 (Continued) Individual Clinical Observations Covance 6329-183 3M T-6295 809 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 265 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F1 DOSE: 0 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92861 1 C92862 3 4 NORMAL NO REMARKABLE OBSERVATIONS 53 APPEARANCE MALOCCLUSION C92863 M EXCRETION NONFORMED FECES NORMAL NO REMARKABLE OBSERVATIONS 83 APPEARANCE MISSING TAIL-DISTAL SKIN & PELAGE BLACK SKIN TAIL NORMAL NO REMARKABLE OBSERVATIONS 1 , 8 , 15, 22 134, 141, 148, 155 , 162, 169, 176, 183 , 190, 197, 204, 211, 218 225, 232, 239, 246 , 253, 260, 267, 274 , 316, 330, 337, 344, 351 358, 365- 366 274 1 , 8 , 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127, 281 , 288, 295, 302, 309 , 323 575 575 1 , 8 , 15 113, 120 204, 211 295, 302 386, 393 477, 484 568 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 400, 407 , 414, 421, 428, 435 , 442, 449, 456, 463 , 470 491, 498 , 505, 512, 519, 526 , 533, 540, 547, 554 , 561 810 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 266 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F1 DOSE: 0 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92864 1 C92865 D 4 NORMAL NO REMARKABLE OBSERVATIONS 65 NORMAL NO REMARKABLE OBSERVATIONS C92866 T MASS(ES) AXILLARY REGION-LEFT WEEK 46 VENTRAL-ABDOMINAL WEEK 41 VENTRAL-CERVICAL-LEFT WEEK 47 105 APPEARANCE LIMITED USE LIMB-HIND-LEFT SWOLLEN PAW-HIND-LEFT EXCRETION FEW FECES NO FECES SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE 1, 8 , 15, 22 1 , 8 ,r 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 337 , 344 351, 358, 365, 372 , 379, 386, 393, 400 , 407, 414, 421, 428 , 435 442, 449 316, 323, 330 281C, 288C, 295C, 302C, 309C, 316C, 323C 323, 330 239, 246 239, 246 715, 717, 722, 729, 733 691 715, 717, 722 811 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 267 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F1 (CONTINUED FROM PREVIOUS PAGE) C92866 T 105 SKIN & PELAGE SORE/SCAB MOUTH NORMAL NO REMARKABLE OBSERVATIONS C92867 T 105 NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) VENTRAL-ABDOMINAL WEEK 104 DOSE: 0 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 8 1 , 15, 22, 29, 36, 43, 50 , 57,r 64, 71, 78, 85, 92, 99, 106, 113 120 , 127, 134, 141,. 148, 155, 162, 169, 176 , 183, 190, 197, 204 211 , 218, 225, 232,. 253, 260, 267, 274, 281 , 288, 295, 302, 309 316 , 323, 330, 337,. 344, 351, 358, 365, 372 , 379, 386, 393, 400 407 , 414, 421, 428, 435, 442, 449, 456, 463 , 470, 477, 484, 491 498 , 505, 512, 519,. 526, 533, 540, 547, 554 , 561, 568, 575, 582 589 , 596, 603, 610, 617, 624, 631, 638, 645 , 652, 659, 666, 673 680 , 687, 694, 701, 708 1 , 8 ,r 15, 2 2 , 29, 36 , 43,r 50, 113, 1 2 0 , 127,. 134, 141, 148, 204, 2 1 1 , 218, 225, 232, 239, 295, 302, 309,. 316, 323, 330, 386, 393, 400, 407, 414, 421, 477, 484, 491, 498, 505, 512, 568, 575, 582, 589, 596, 603, 659, 666, 673, 680, 687, 694, 57, 64, 71, 78, 85, 155, 162 , 169, 176, 246, 253 , 260, 267, 337, 344 , 351, 358, 428, 435 , 442, 449, 519, 526 , 533, 540, 610, 617 , 624, 631, 701, 708 , 715 92, 99, 106 183, 190 , 197 274, 281 , 288 365, 372 , 379 456, 463 , 470 547, 554 , 561 638, 645 , 652 722C, 72 9C, 732C 812 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 268 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F1 DOSE: 0 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92868 T 105 NORMAL NO REMARKABLE OBSERVATIONS T 105 NORMAL NO REMARKABLE OBSERVATIONS C92870 M 105 APPEARANCE HEAD TILT LEFT MALOCCLUSION SWOLLEN LIMB-HIND-LEFT PAW-HIND-RIGHT THIN BEHAVIOR ATAXIC <UNSPECIFIED> 1 , 8 ,r 15, 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582, 589, 596, 603, 610, 617 , 624, 631, 638, 645 , 652 659, 666, 673, 680, 687, 694, 701, 708 , 715, 722, 729, 731 1 , 8 ,r 15, 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582, 589, 596, 603, 610, 617 , 624, 631, 638, 645 , 652 659, 666, 673, 680, 687, 694, 701, 708 , 715, 722, 729, 732 729 246, 253, 260, 267, 645, 652, 722, 729 645 673 729 729 813 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 269 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F1 (CONTINUED FROM PREVIOUS PAGE) C92870 M 105 BEHAVIOR HYPOACTIVE <UNSPECIFIED> RECUMBENT LATERAL EXCRETION FEW FECES EYES RED DISCHARGE EYE-RIGHT NORMAL NO REMARKABLE OBSERVATIONS s.SS(ES) AXILLARY REGION-LEFT WEEK 99 INGUINAL REGION-LEFT WEEK 105 DOSE: 0 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 729 729 717, 722, 729 717 1 , 8 , 15 113, 120 204, 211 323, 330 414, 421 505, 512 596, 603 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 218, 225, 232, 239, 274, 281 , 288, 295, 302, 309 , 316 337,. 344, 351, 358, 365, 372 , 379, 386, 393, 400 , 407 428, 435, 442, 449, 456, 463 , 470, 477, 484, 491 , 498 519,. 526, 533, 540, 547, 554 , 561, 568, 575, 582 , 589 610, 617, 624, 631, 638, 659 , 666, 680 687, 694 701,. 708, 715, 717, 722, 729 729 814 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 270 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F1 DOSE: 0 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92871 T 105 NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) INGUINAL REGION-LEFT WEEK 94 VENTRAL-ABDOMINAL WEEK 84 VENTRAL-THORACIC-LEFT WEEK 76 C92872 M 89 SKIN & PELAGE SORE/SCAB VENTRAL-ABDOMINAL-LEFT NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) INGUINAL REGION-LEFT WEEK 83 1 , 8 ,r 15, 2 2 , 29, 36 , 43,r 50, 113, 1 2 0 , 127,. 134, 141, 148, 204, 2 1 1 , 218, 225, 232, 239, 295, 302, 309,. 316, 323, 330, 386, 393, 400, 407, 414, 421, 477, 484, 491, 498, 505, 512, 57, 64, 71, 78, 85, 155, 162 , 169, 176, 246, 253 , 260, 267, 337, 344 , 351, 358, 428, 435 , 442, 449, 519 92, 99, 106 183, 190 , 197 274, 281 , 288 365, 372 , 379 456, 463 , 470 652C, 659C, 666C, 673C, 680C, 687C, 694C, 701C, 708C, 715C, 722C 729C, 731C 582 526C, 533C, 54 0C, 54 7C, 554C, 561C, 568C, 575C, 582C, 58 9C, 596C 603C, 610C, 617C, 624C, 631C, 638C, 645C, 652C, 659C, 666C, 673C 680C, 687C, 694C, 701C, 708C, 715C, 722C, 729C, 731C 617-618 1 , 8 ,r 15, 2 2 , 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127,. 134, 141, 148, 155 , 162, 169, 176, 183,. 190, 197 204, 2 1 1 , 218,. 225, 232, 239, 246 , 253, 260, 267, 274 575, 582, 589, 596C, 603C , 610C, 617-618C 815 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 271 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F1 (CONTINUED FROM PREVIOUS PAGE) C92872 M 89 MASS(ES) VENTRAL-ABDOMINAL WEEK 41 C92873 T 105 SKIN & PELAGE ALOPECIA NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) AXILLARY REGION-LEFT WEEK 76 WEEK 79 DOSE: 0 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 281, 288, 295, 302 , 309,, 316, 365C, 372C , 379C, 386C, 393C, 442C, 449C , 456C, 463C, 470C, 519C, 526C , 533C, 54 0C, 54 7C, 596C, 603C, 610C, 617-618C 323C, 400C, 477C, 554C, 330C, 407C, 484C, 561C, 337C, 414C, 491C, 568C, 344C, 421C, 498C, 575C, 351C, 428C, 505C, 582C, 358C 435C 512C 589C 330, 337, 344, 351, 358, 365, 372, 379 , 386, 393, 400, 407 , 414 421, 428, 435,. 442, 449, 456, 463, 470 , 554, 561, 568, 575 , 582 589, 596, 610, 617, 624, 631, 638, 645 , 652, 659, 666, 673 , 680 687, 694, 701,. 708, 715, 722, 729, 733 1 , 8 ,r 15, 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 477, 484, 491 , 498, 505, 512, 519 , 533 540, 603 526 54 7C, 554C, 561C, 568C 816 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 272 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F1 DOSE: 0 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92874 T 105 NORMAL NO REMARKABLE OBSERVATIONS C92875 M MASS(ES) VENTRAL-ABDOMINAL WEEK 93 79 APPEARANCE MALOCCLUSION THIN BEHAVIOR HYPOACTIVE <UNSPECIFIED> DISCHARGE NASAL RED IN COLOR EXCRETION FEW FECES 1 , 8 , 15, 2 2 , 29, 36, 43,r 50, 113, 1 2 0 , 127, 134 , 141, 148, 204, 2 1 1 , 218, 225 , 232, 239, 295, 302, 309, 316 , 323, 330, 386, 393, 400, 407 , 414, 421, 477, 484, 491, 498 , 505, 512, 568, 575, 582, 589 , 596, 603, 57, 64, 71 , 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 92, 99, 106 183, 190 , 197 274, 281 , 288 365, 372 , 379 456, 463 , 470 547, 554 , 561 638 645, 652C, 659C, 666C, 673C, 680C, 687C, 694C , 701C, 708C, 715C 722C,, 72 9C , 731C 421, 428, 435, 442 , 449, 456, 463, 470, 477, 484, 491, 498 , 505 512, 519, 526, 533 , 540, 547, 553 553 553 553 547, 553 817 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 273 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F1 (CONTINUED FROM PREVIOUS PAGE) C92875 M 79 SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE YELLOW HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS C92876 3 53 NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 53 SURGERY PERFORMED BY PATTIE MURPHY ANTIBIOTIC ADMINISTERED 0.5 ML DOSE: 0 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 505, 519, 526, 533 , 540, 547, 553 553 1 , 8 ,r 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127, 134 , 141, 148, 155,. 162, 169, 176, 183,. 190, 197 204, 2 1 1 , 218, 225 , 232, 239, 246,. 253, 260, 267, 274, 281, 288 295, 302, 309, 316 , 323, 330, 337,. 344, 351, 358, 365,. 372, 379 386, 393, 400, 407 , 414 1 , 8 ,r 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127, 134 , 141, 148, 155,. 162, 169, 176, 183,. 190, 197 204, 2 1 1 , 218, 225 , 232, 239, 246,. 253, 260, 267, 274, 281, 288 295, 302, 309, 316 , 323, 330, 337,. 344, 351, 358 365-366C 359-360 818 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 274 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F1 DOSE: 0 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92877 T 105 APPEARANCE MALOCCLUSION NORMAL NO REMARKABLE OBSERVATIONS C92878 M 81 APPEARANCE MALOCCLUSION THIN EYES DISCOLORED/LIGHT RED EYE-LEFT NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 60 99 112,08,,r12175,, 211, 218, 302, 309, 393, 400, 484, 491, 575, 582, 666, 673, 22, 29, 36 , 43,r 50, 134, 141, 148, 155, 225,. 232, 239, 246, 316,. 323, 330, 337, 407,. 414, 421, 428, 498, 505, 512, 519, 589,. 596, 603, 610, 680, 687, 694, 701, 57, 64, 71, 78, 85, 162, 169 , 176, 183, 253, 260 , 267, 274, 344, 351 , 358, 365, 435, 442 , 449, 456, 526, 533 , 540, 547, 617, 624 , 631, 638, 708, 715 , 722, 729, 92, 106, 113 190, 197, 204 281, 288, 295 372, 379, 386 463, 470, 477 554, 561, 568 645, 652, 659 732 169, 260, 351, 442, 533, 519, 176, 267, 358, 449, 540, 526, 183, 274, 365, 456, 547, 562 190, 281, 372, 463, 554, 197, 204, 288, 295, 379, 386, 470, 477, 561- 562 211, 218, 302, 309, 393, 400, 484, 491, 225, 316, 407, 498, 232, 323, 414, 505, 239, 330, 421, 512, 246, 337, 428, 519, 253 344 435 526 526 1 22, 8,, 15, , 29, 36, 43,, 50, 57, 64, 71, 78, 85, 92 120113, , 127,, 134, 141, 148, 155, 162 414C, 421C, 42 8C, 435C, 442C, 449C, 456C, 463C, 470C, 477C, 484C 819 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 275 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F1 (CONTINUED FROM PREVIOUS PAGE) C92878 M 81 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 60 VENTRAL-THORACIC WEEK 45 C92879 M 93 APPEARANCE HEAD TILT LEFT HUNCHED THIN BEHAVIOR ATAXIC <UNSPECIFIED> EYES RED DISCHARGE EYES NORMAL NO REMARKABLE OBSERVATIONS DOSE: 0 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 491C , 498C, 505C, 512C , 519C, 52 6C, 53 3C, 54 0C, 54 7C, 554C 561- 562C 309C , 316C, 386C , 393C, 463C , 470C, 540C , 547C, 32 3C, 400C, 477C, 554C, 330C , 337C, 407C , 414C, 484C , 491C, 561- 562C 344C, 421C, 498C, 351C, 42 8C, 5 05C, 358C, 435C, 512C, 365C, 442C, 519C, 372C, 44 9C, 52 6C, 379C 456C 533C 645 603, 610 596, 603, 610, 617, 624, 631, 638, 645 638, 645 610 1 , 8 ,r 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 351 , 358, 365, 372, 379 , 386 393, 400, 407, 414 , 421, 428, 435, 442 820 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 276 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F1 (CONTINUED FROM PREVIOUS PAGE) C92879 M 93 MASS(ES) AXILLARY REGION-LEFT WEEK 69 2 C92881 T INGUINAL REGION-LEFT WEEK 65 INGUINAL REGION-RIGHT WEEK 50 VENTRAL-ABDOMINAL-RIGHT WEEK 65 RECTUM WEEK 92 14 NORMAL NO REMARKABLE OBSERVATIONS 105 APPEARANCE MALOCCLUSION MISSING TEETH SWOLLEN DIGIT(S)-HIND-LEFT DOSE: 0 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 477C, 484C, 491C, 498C, 505C, 512C, 519C, 52 6C, 53 3C, 54 0C, 547C 554C, 561C, 56 8C, 575C, 582C, 58 9C, 596C, 603C, 610C, 617C, 624C 631C, 638C, 645C 44 9C, 456C, 463C, 470C, 477C, 484C, 491C, 498C, 505C, 512C, 519C 52 6C, 53 3C, 540C, 547C, 554C, 561, 568 344C 449, 456, 463, 470 , 477C, 484C , 491C 638C, 645C 1 , 8 ,. 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92-93 134, 225, 316, 407, 498, 589, 680, 141, 232, 323, 414, 505, 596, 687, 148, 239, 330, 421, 512, 603, 694, 155, 246, 337, 428, 519, 610, 701, 162, 253, 344, 435, 526, 617, 708, 169, 260, 351, 442, 533, 624, 715, 176, 267, 358, 449, 540, 631, 722, 183, 274, 365, 456, 547, 638, 729, 190, 281, 372, 463, 554, 645, 733 197, 288, 379, 470, 561, 652, 204, 295, 386, 477, 568, 659, 2 1 1 , 218 302, 309 393, 400 484, 491 575, 582 666, 673 596, 603, 610, 617, 624, 631, 638, 645, 652, 659, 666, 673, 680 687, 694, 701, 708, 715, 722, 729, 733 722 821 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 277 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F1 (CONTINUED FROM PREVIOUS PAGE) C92881 T 105 EXCRETION FEW FECES EYES DISCOLORED/LIGHT RED EYE-RIGHT NORMAL NO REMARKABLE OBSERVATIONS C92882 T MASS(ES) AXILLARY REGION-RIGHT WEEK 96 INGUINAL REGION-LEFT WEEK 99 NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) AXILLARY REGION-LEFT WEEK 69 AXILLARY REGION-RIGHT WEEK 100 DOSE: 0 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 526 414 1 , 8 , 15, 22, 29, 36, 43 , 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0, 127 666, 673, 680, 687, 694, 701 687C, 694C , 701C, 708C, 715C, 722C , 729C, 733C 1 , 8 , 15, 22, 29, 36, 43 , 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0, 127, 134, 141, 148, 155, 162, 169, 176, 183,. 190, 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365,. 372, 379 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456,. 463, 470 477C, 484C, 491C, 498C, 505C, 512C, 519C, 526C, 533C, 54 0C, 547C 554C, 561C, 568C, 575C, 582C, 58 9C, 596C, 603C, 610C, 617C, 624C 631C, 638C, 645C, 652C, 659C, 666C, 673C, 680C, 687C, 694C, 701C 708C, 715C, 722C, 729C, 733C 694C, 701C, 708C, 715C, 722C, 729C, 733C 822 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 278 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F1 DOSE: 0 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92883 M 87 APPEARANCE MALOCCLUSION BEHAVIOR ATAXIC <UNSPECIFIED> DISCHARGE NASAL RED IN COLOR EXCRETION NO FECES NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) AXILLARY REGION-RIGHT WEEK 81 INGUINAL REGION-RIGHT WEEK 75 64, 71, 78, 85,r 92, 99, 106, 113, 1 2 0, 127, 134, 141, 148, 155 162, 169 , 176, 183, 190, 197, 204, 2 1 1 , 218, 225, 232, 239, 246 253, 260 , 267, 274, 281,. 288, 295, 302, 309, 316, 323, 330, 337 344, 351 , 358, 365, 372, 379, 386, 393, 400, 407, 414, 421, 428 435, 442 , 449, 456, 463,. 470, 477, 484, 491, 498, 505,. 512, 519 526, 533 , 540, 547, 554, 561, 568, 575, 582, 589, 596, 603- 604 603-604 64 604 1, 8 , 15, 22, 29, 36, 43, 50, 57 561, 568 519C, 526C, 533C, 540C, 547C, 554C, 561C, 568C, 575C, 582C, 589C 596C, 603-604C 823 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 279 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F1 DOSE: 0 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92884 M 96 APPEARANCE CONVULSIONS THIN EXCRETION FEW FECES EYES PROTRUDING EYES SKIN & PELAGE PALE ENTIRE BODY SORE/SCAB AXILLARY REGION-RIGHT NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) AXILLARY REGION-LEFT WEEK 73 WEEK 89 AXILLARY REGION-RIGHT WEEK 85 VENTRAL-ABDOMINAL-LEFT WEEK 79 652 624, 631, 638 , 645, 652, 659, 666 624, 631 519, 526, 533,. 540, 547, 554, 561, 568 , 575, 582, 652, 659 , 666 666 666 1 , 8 ,r 15, 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442 505C 617C, 624C, 631C, 638C, 645C, 652C, 659C, 666C 58 9C, 596C 547, 554, 561, 568, 575,. 582, 589, 596, 603, 610, 617C, 624C 824 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 280 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F1 (CONTINUED FROM PREVIOUS PAGE) C92884 M 96 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 79 VENTRAL -ABDOMINAL-RIGHT WEEK 65 WEEK 74 C92885 T 105 NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) INGUINAL REGION-LEFT WEEK 99 VENTRAL-ABDOMINAL WEEK 15 DOSE: 0 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 631C, 638C, 64 5C, 652C, 659C, 666C 44 9C, 52 6C, 603C, 512C, 58 9C, 666C 456C, 53 3C, 610C, 519C, 5 96C, 463C, 54 0C, 617C, 52 6C, 603C, 4 7 0C, 54 7C, 624C, 53 3C, 610C, 477C, 554C, 631C, 54 0C, 617C, 484C, 561C, 638C, 54 7C, 624C, 491C, 56 8C, 64 5C, 554C, 631C, 498C, 575C, 652C, 561C, 638C, 5 05C, 582C, 6 5 9C, 56 8C, 64 5C, 512C, 58 9C, 666C 575C, 652C, 519C 596C 582C 659C 1 , 8 ,r 15, 134, 141, 225, 232, 316, 323, 407, 414, 498, 505, 589, 596, 680 2 2 , 29, 36 , 43,r 50, 148,. 155, 162, 169, 239,. 246, 253, 260, 330, 337, 344, 351, 421,. 428, 435, 442, 512, 519, 526, 533, 603,. 610, 617, 624, 57, 64, 71, 78, 85, 176, 183 , 190, 197, 267, 274 , 281, 288, 358, 365 , 372, 379, 449, 456 , 463, 470, 540, 547 , 554, 561, 631, 638 , 645, 652, 92, 1 2 0, 127 204, 2 1 1 , 218 295, 302, 309 386, 393, 400 477, 484, 491 568, 575, 582 659, 666, 673 687,. 694, 701, 708C, 715C, 722C, 729C, 733C 99, 106, 113 825 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 281 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F1 DOSE: 0 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92886 M 81 APPEARANCE SWOLLEN VENTRAL-ABDOMINAL THIN EYES DISCOLORED/LIGHT RED EYES RED DISCHARGE EYE-RIGHT SKIN & PELAGE BLUE SKIN VENTRAL-ABDOMINAL SORE/SCAB AXILLARY REGION-LEFT NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) AXILLARY REGION-LEFT WEEK 59 INGUINAL REGION-RIGHT WEEK 59 554, 561 561 554, 561 526 561 477, 484, 491, 498 , 505, 512, 519, 526 , 533, 540, 547, 554 , 561 1 , 8 ,r 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400 407, 414, 421C, 428C, 435C, 442C, 44 9C, 456C, 463C, 470C, 477C 484C, 491C , 498C , 505C , 512C , 519C , 526C , 533C 407C, 414C , 421C , 428C , 435C , 442C , 449C , 456C, 463C 826 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 282 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F1 DOSE: 0 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92887 2 C92888 M 14 NORMAL NO REMARKABLE OBSERVATIONS 79 APPEARANCE HEAD TILT RIGHT BEHAVIOR HYPOACTIVE <UNSPECIFIED> EXCRETION FEW FECES EYES RED DISCHARGE EYE-RIGHT NORMAL NO REMARKABLE OBSERVATIONS 1, 8 , 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92-93 551, 553 553 553 519, 526, 533,. 540 , 547, 551, 553 1 , 8 ,r 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407 , 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498 , 505, 512 827 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 283 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F1 DOSE: 0 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92889 3 53 NORMAL NO REMARKABLE OBSERVATIONS C92890 T SURGERY PERFORMED BY PATTIE MURPHY ANTIBIOTIC ADMINISTERED 0.5 ML 105 APPEARANCE MALOCCLUSION NORMAL NO REMARKABLE OBSERVATIONS 1 , 8 ,r 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99 106 113, 1 2 0, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 1 90 , 197 204, 2 1 1 , 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365-36 6 359 359-360 638, 645 652, 659, 666, 673, 680, 687 , 694, 701, 708, 715 , 722 729, 733 1 , 8 , 15 113, 120 204, 211 295, 302 386, 393 477, 484 568, 575 2 2 , 29, 36 , 43,r 50, 127,. 134, 141, 148, 218, 225, 232, 239, 309,. 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 57, 64, 71, 78, 85, 155, 162 , 169, 176, 246, 253 , 260, 267, 337, 344 , 351, 358, 428, 435 , 442, 449, 519, 526 , 533, 540, 610, 617 , 624, 631 92, 99, 106 183, 190 , 197 274, 281 , 288 365, 372 , 379 456, 463 , 470 547, 554 , 561 828 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 284 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F1 DOSE: 0 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92891 M 85 APPEARANCE HEAD TILT LEFT THIN BEHAVIOR RECUMBENT LATERAL EXCRETION FEW FECES RESPIRATION AUDIBLE IRREGULAR NORMAL NO REMARKABLE OBSERVATIONS 582, 589, 591 591 591 591 591 591 1 , 8 ,r 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407 , 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498 , 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575 829 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 285 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F1 DOSE: 0 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92892 T 105 APPEARANCE OBES NORMAL NO REMARKABLE OBSERVATIONS C92893 M 88 APPEARANCE HUNCHED MALOCCLUSION THIN BEHAVIOR SENSITIVE TO TOUCH SKIN & PELAGE ALOPECIA ROUGH HAIR COAT SORE/SCAB VENTRAL-THORACIC-LEFT 680, 687 694, 701, 708, 715, 722, 729 , 732 1 , 8 , 15 113, 120 204, 211 295, 302 386, 393 477, 484 568, 575 659, 666 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 582, 589, 596, 603, 610, 617 , 624, 631, 638, 645 , 652 673 610 274, 365, 456, 547, 337, 281 372 463 554 344 288, 379, 470, 561, 351, 295, 386, 477, 568, 358 302, 393, 484, 575, 309, 400, 491, 582, 316, 407, 498, 589, 323, 414, 505, 596, 330, 421, 512, 603, 337, 428, 519, 610 344, 435, 526, 351, 442, 533, 358 449 540 337 365, 372 379, 386, 393, 400, 407, 414, 421, 428, 435, 442, 449 456, 463 393, 400 407, 470, 477, 484, 491, 498, 505, 512, 519, 526, 554 603, 610 830 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 286 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F1 (CONTINUED FROM PREVIOUS PAGE) C92893 M 88 NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) AXILLARY REGION-RIGHT WEEK 74 INGUINAL REGION-RIGHT WEEK 76 VENTRAL-ABDOMINAL-RIGHT WEEK 76 VENTRAL-THORACIC-LEFT WEEK 74 RECTUM WEEK 61 3 53 NORMAL NO REMARKABLE OBSERVATIONS DOSE: 0 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 1 , 8 ,. 15, 22, 29, 36, 43 , 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162, 169, 176, 183 , 190, 197 204, 2 1 1 , 218,. 225, 232, 239, 246, 253, 260, 267 512, 519C, 526C, 533C, 540C, 547, 554, 561C, 568C, 575C, 582C 58 9C, 596C, 603C, 610C 526C,. 533C, 540C, 54 7C, 554C 526C,. 533C, 540C, 54 7C, 554C, 561C , 568C 512, 519C, 526C, 533C, 540C, 54 7C, 554C, 561C , 568C, 575C, 582C 58 9C, 596C, 603C, 610C 421C, 428C, 435C, 442C, 44 9C, 456C , 463C 1 , 8 ,. 15, 22, 29, 36, 43 , 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162, 169, 176, 183 , 190, 197 204, 2 1 1 , 218,. 225, 232, 239, 246, 253, 260, 267, 274 , 281, 288 295, 302, 309,. 316, 323, 330, 337, 344, 351, 358, 365 -366 831 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 287 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F1 DOSE: 0 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92895 T 105 NORMAL NO REMARKABLE OBSERVATIONS C92896 T MASS(ES) AXILLARY REGION-LEFT WEEK 87 NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) INGUINAL REGION-RIGHT WEEK 76 VENTRAL-ABDOMINAL-RIGHT WEEK 97 VENTRAL-THORACIC WEEK 104 1, 8,. 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281 , 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526, 533, 540, 547, 554 , 561 568, 575, 582, 589, 596 666603C, 610C, 617C, 624C, 631C, 638C , 645C, 652C, 659C, C , 673C 680C,. 687C, 694C, 701C, 708C, 715C , 722C, 729C, 732C 1, 8,. 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281 , 288 295, 302, 309, 323, 330, 337, 344, 351, 358, 365, 372, 379 , 386 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470 , 477 484, 491, 498, 505, 512, 519 526C,. 533C, 540C, 547C, 554C, 561C , 568C, 575C, 582C, 589C , 596C 666603C, 610C, 617C, 624C, 631C, 638C , 645C, 652C, 659C, C , 673C 680C,. 687C, 694C, 701C, 708C, 715C , 722C, 729C, 731C 673C,. 680C, 687C, 694C, 701C, 708C , 715C 722, 729, 731 832 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 288 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F1 (CONTINUED FROM PREVIOUS PAGE) C92896 T 105 WART LIKE LESION(S) MOUTH WEEK 46 C92897 T 105 APPEARANCE THIN EXCRETION FEW FECES EYES SQUINTED PERI-ORBITAL NORMAL NO REMARKABLE OBSERVATIONS 2 14 NORMAL NO REMARKABLE OBSERVATIONS DOSE: 0 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 316 722, 729-731 730-731 687, 730- 731 1 , 8 ,r 15, 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127,. 134, 141, 148, 155, 162 , 169, 176, 183 , 190 , 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253 , 260, 267, 274 , 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365 , 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456 , 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547 , 554 , 561 568, 575, 582, 589, 596, 603, 610, 617 , 624, 631, 638 , 645 , 652 659, 666, 673, 680, 694, 701, 708, 715 1 , 8 ,r 15, 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92- 93 833 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 289 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F1 DOSE: 0 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92899 3 53 SKIN & PELAGE SORE/SCAB PAW-HIND-LEFT NORMAL NO REMARKABLE OBSERVATIONS C92900 D SURGERY PERFORMED BY JOHN VAN BERKUM ANTIBIOTIC ADMINISTERED 0.5 ML 72 APPEARANCE MALOCCLUSION MISSING TEETH EXCRETION NONFORMED FECES 365-366 1 , 8 ,r 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127, 134 , 141, 148, 155, 162 , 169, 176, 183,. 190, 197 204, 2 1 1 , 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281, 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358 359 359-360 85, 92, 99, 106 , 113 , 120 , 127 , 134 , 141 , 148 , 155 , 162 , 169 176, 183 , 190, 197, 204, 2 1 1 , 218, 225, 232, 239, 246, 253, 260 267, 274 , 281, 288, 295, 302, 309, 316, 323, 330, 337, 344, 351 358, 365 , 372, 379, 386, 393, 400, 407, 414, 421, 428, 435, 442 449, 456 , 463, 470, 477, 484, 491, 498, 504 176, 183 , 190, 197, 204, 2 1 1 , 218, 225, 232, 239, 246, 253, 260 267, 274 , 281, 288, 295, 302, 309, 316, 323, 330, 337, 344, 351 358, 365 , 372, 379, 386, 393, 400, 407, 414, 421, 428, 435, 449 456, 463 , 470, 477, 484, 491, 498, 504 225 834 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 290 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F1 (CONTINUED FROM PREVIOUS PAGE) C92900 D 72 EYES CLEAR DISCHARGE EYE-LEFT SKIN & PELAGE YELLOW HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS C92901 M 79 APPEARANCE HEAD TILT LEFT BEHAVIOR ATAXIC <UNSPECIFIED> DISCHARGE NASAL RED IN COLOR EXCRETION FEW FECES EYES RED DISCHARGE EYE-LEFT SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE DOSE: 0 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 393 85 1, 8 , 15, 22, 29, 36, 43, 50, 57, 64, 71, 78 533, 540, 547, 551 551 551 526, 533, 540, 547, 551 547, 551 519, 526, 533, 540 835 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 291 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F1 (CONTINUED FROM PREVIOUS PAGE) C92901 M 79 NORMAL NO REMARKABLE OBSERVATIONS C92902 D MASS(ES) AXILLARY REGION-LEFT WEEK 48 AXILLARY REGION-RIGHT WEEK 48 VENTRAL-ABDOMINAL WEEK 61 RECTUM WEEK 76 89 APPEARANCE OBES NORMAL NO REMARKABLE OBSERVATIONS DOSE: 0 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 1 , 8 ,r 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190, 197 204, 2 1 1 , 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281, 288 295, 302, 309, 316 , 323, 414, 470, 477 , 484, 491, 498, 505, 512 330 330, 337, 344, 351, 358, 365, 372, 379, 386, 393, 400, 407 421, 428, 435, 442, 449,. 456, 463 526C, 533C , 540C, 547C, 551C 526, 533, 540, 547, 554, 561, 568, 575 , 582, 589, 596, 603 1 , 8 ,r 15, 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 610 , 617 836 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 292 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F1 DOSE: 0 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92903 2 C92904 M 14 NORMAL NO REMARKABLE OBSERVATIONS 101 SKIN & PELAGE SORE/SCAB VENTRAL-CERVICAL-LEFT VENTRAL-THORACIC NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) AXILLARY REGION-LEFT WEEK 69 INGUINAL REGION-RIGHT WEEK 70 VENTRAL-ABDOMINAL-LEFT WEEK 97 VENTRAL-CERVICAL-LEFT WEEK 63 1 , 8 15, 22, 29, 36, 43 50 57, 64, 71, 78 85 92-93 610 638 1 , 8 ,r 15, 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428 477, 484C, 491C, 498C, 505C, 512C, 519C, 526C, 533C, 54 0C, 547C 554C , 561C , 568C , 575C, 582C , 589C, 596C , 603C , 610C, 617C , 624C 631C , 638C , 645C , 652C, 659C , 666C, 673C , 680C , 687C, 694C 701- 702C 484C , 491C , 498C , 505C, 512C , 519C, 526C , 533C , 540C, 547C , 554C 561C , 568C , 575C , 582C, 589C , 596C, 603C , 610C , 617C, 624C , 631C 638C , 645C , 652C , 659C, 666C , 673C, 680C , 687C , 694C, 701- 702C 673C , 680C , 687C , 694C, 701- 702C 435, 442, 449C, 456C, 463C, 470C, 477C, 484C, 491C, 498C, 505C 512C , 519C , 526C , 533C, 540C , 547C, 554C , 561C , 568C, 575C , 582C 589C , 596C , 603, 610, 617, 624, 631 , 638 , 645 837 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 293 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F1 (CONTINUED FROM PREVIOUS PAGE) C92904 M 101 MASS(ES) VENTRAL-THORACIC-LEFT WEEK 73 WART LIKE LESION(S) MOUTH WEEK 89 C92905 2 C92906 T 14 NORMAL NO REMARKABLE OBSERVATIONS 105 APPEARANCE MALOCCLUSION EYES CLEAR DISCHARGE EYE-RIGHT RED DISCHARGE EYES SKIN & PELAGE SORE/SCAB MOUTH NORMAL NO REMARKABLE OBSERVATIONS DOSE: 0 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 505C, 512C, 519C, 526C, 533C, 540C , 547C, 554C, 561C, 568C, 575C 582C, 589C, 596C, 603C, 610C, 617C , 624C, 631C, 638C, 645C, 652C 659C, 666C, 673C, 680C, 687C, 694C , 701-702C 617, 624, 631, 638 , 645, 652, 659, 666, 673, 680, 687 , 694 701-702 1, 8 , 15, 22, 29, 36, 43 , 50, 57, 64, 71, 78,. 85, 92- 93 631, 638, 645, 652 , 659, 666, 673, 680, 687, 694, 701 , 708, 715 722, 729, 731 519, 526 610, 617 631, 638, 645 1 , 8 ,r 15, 22, 29, 36, 43,, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127, 134, 141, 148, 155,. 162, 169, 176, 183,. 190, 197 204, 2 1 1 , 218, 225, 232, 239, 246,. 253, 260, 267, 274, 281, 288 838 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 294 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F1 (CONTINUED FROM PREVIOUS PAGE) C92906 T 105 NORMAL NO REMARKABLE OBSERVATIONS C92907 T MASS(ES) AXILLARY REGION-LEFT WEEK 95 AXILLARY REGION-RIGHT WEEK 70 WEEK 95 VENTRAL-ABDOMINAL-RIGHT WEEK 65 NORMAL NO REMARKABLE OBSERVATIONS DOSE: 0 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421, 428, 435, 442, 498, 505, 512, 533 540, 547, 554, 561, 568, 575, 582, 589, 596, 603, 624 659C, 666C , 673C, 680C, 687C, 694C , 701C, 708C, 715C, 722C , 72 9C 731C 484, 491 659C, 666C , 673C, 680C, 687C, 694C , 701C, 708C, 715C, 722C , 729C 731C 449, 456, 463,. 470, 477, 484, 491 1 , 8 ,. 15, 2 2 , 29, 36, 43 , 50, 57, 64, 71, 78, 85 , 92, 99, 106 113, 1 2 0 , 127,. 134, 141, 148, 155, 162, 169, 176 , 183, 190 , 197 204, 2 1 1 , 218,. 225, 232, 239, 246, 253, 260, 267 , 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344, 351, 358 , 365, 372 , 379 386, 393, 400,. 407, 414, 421, 428, 435, 442, 449 , 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526, 533, 540 , 547, 554 , 561 568, 575, 582,. 589, 596, 603, 610, 617, 624, 631 , 638, 645 , 652 659, 666, 673, 680, 687, 694, 701, 708, 715, 722 , 729, 733 839 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 295 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F1 DOSE: 0 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92908 M 93 BEHAVIOR ATAXIC <UNSPECIFIED> HYPOACTIVE <UNSPECIFIED> RECUMBENT LATERAL EXCRETION FEW FECES NO FECES EYES RED DISCHARGE EYES SKIN & PELAGE BROWN HAIR COAT PERINEAL AREA NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) VENTRAL-CERVICAL WEEK 78 631, 646 631, 646 646 638, 645-646 624, 631 624, 631 624, 631 1 , 8 , 15, 2 2 , 29, 36, 43 , 50 , 57, 64, 71 , 78, 85 , 92, 99, 106 113, 1 2 0 , 127,. 134, 141, 148 , 155, 162, 169, 176 , 183, 190 , 197 204, 2 1 1 , 218,. 225, 232, 239 , 246, 253, 260, 267 , 274, 281 , 288 295, 302, 309,. 316, 323, 330 , 337, 344, 351, 358 , 365, 372 , 379 386, 393, 400,. 407, 414, 421 , 428, 435, 442, 449 , 456, 463 , 470 477, 484, 491, 498, 505, 512 , 519, 526, 533 540C, 547C , 554C, 561C, 568C , 575C , 582C , 589C, 596C, 603C , 610C 617C, 624C , 631C, 638C, 645- 646C 840 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 296 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F1 DOSE: 0 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92909 T 105 NORMAL NO REMARKABLE OBSERVATIONS C92910 3 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 98 53 APPEARANCE MALOCCLUSION DISCHARGE NASAL RED IN COLOR NORMAL NO REMARKABLE OBSERVATIONS 1 , 8 , 15, 2 2 , 29, 36, 43 , 50, 57, 64, 71 , 78, 85 , 92, 99, 10 6 113, 1 2 0 , 127,. 134, 141, 148, 155, 162, 169, 176 , 183, 190, 197 204, 2 1 1 , 218,. 225, 232, 239, 246, 253, 260, 267 , 274, 281, 288 295, 302, 309,. 316, 323, 330, 337, 344, 351, 358 , 365, 372, 379 386, 393, 400,. 407, 414, 421, 428, 435, 442, 449 , 456, 463, 470 477, 484, 491, 498, 505, 512, 519, 526, 533, 540 , 547, 554, 561 568, 575, 582,. 589, 596, 603, 610, 617, 624, 631 , 638, 645, 652 659, 666, 673 680C, 687C!, 694C, 701C, 708C, 715C , 722C , 729C, 732C 108, 113, 1 2 0 ,. 127, 134, 141, 148, 155, 162, 169 , 176, 183, 190 197, 204, 2 1 1 ,. 218, 225, 232, 239, 246, 253, 260 , 267, 274, 281 288, 295, 302,. 309, 316, 323, 330, 337, 344, 351 , 358, 365-3 66 108, 113 1 , 8 , 15, 2 2 , 29, 36, 43 , 50, 57, 64, 71 , 78, 85 , 92, 99, 10 6 841 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 297 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F1 DOSE: 0 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92911 T 105 APPEARANCE MALOCCLUSION EYES CLEAR DISCHARGE EYE-LEFT SKIN & PELAGE ROUGH HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) AXILLARY REGION-LEFT WEEK 69 AXILLARY REGION-RIGHT WEEK 91 VENTRAL-ABDOMINAL WEEK 65 VENTRAL-ABDOMINAL-RIGHT WEEK 60 582, 589, 596, 603, 631, 638, 645, 652 , 673, 680, 687, 694 , 715 722, 729, 732 526 239 1 , 8 ,r 15, 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225, 232, 246, 253, 260 , 267, 274, 281, 288 , 295 302, 309, 316,. 323, 330, 337, 344, 351 , 358, 365, 372, 379 , 386 393, 400, 407, 421, 428, 435, 442, 470 , 547, 554, 561, 568 , 575 610, 617, 624, 659, 666, 701, 708 477C, 484C , 491C, 498C, 505C, 512C, 519C, 526C, 533, 540 631, 638, 645 449, 456, 463 414 842 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 298 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F1 DOSE: 0 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92912 T 105 SKIN & PELAGE BROWN HAIR COAT PERINEAL AREA SORE/SCAB VENTRAL-ABDOMINAL NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 80 C92913 T 105 SKIN & PELAGE BLACK SKIN VENTRAL-ABDOMINAL SORE/SCAB VENTRAL-ABDOMINAL NORMAL NO REMARKABLE OBSERVATIONS 645 722, 729, 732 1 , 8 , 15, 22, 29, 36, 43 , 50, 113, 1 2 0 , 127, 134, 141, 148, 204, 2 1 1 , 218, 225, 232, 239, 295, 302, 309, 316, 323, 330, 386, 393, 400, 407, 414, 421, 477, 484, 491, 498, 505, 512, 57, 64, 71 , 78, 85 , 92, 99, 106 155, 162, 169, 176 , 183 , 190 , 197 246, 253, 260, 267 , 274 , 281 , 288 337, 344, 351, 358 , 365 , 372 , 379 428, 435, 442, 449 , 456 , 463 , 470 519, 526, 533, 540 , 547 554, 561C, 568C, 575C, 582C, 58 9C, 596C, 603C, 610C, 617C, 624C 631C, 638C , 645C, 652C, 659C, 666C , 673C , 680C, 687C, 694C , 701C 708C, 715C , 722C, 729C, 732C 715, 722 729, 731 1 , 8 ,r 15, 2 2 , 29, 36, 43,r 50, 113, 1 2 0 , 127, 134 , 141, 148, 204, 2 1 1 , 218, 225 , 232, 239, 295, 302, 309, 316 , 323, 330, 386, 393, 400, 407 , 414, 421, 57, 64, 71, 78, 85, 155, 162 , 169, 176, 246, 253 , 260, 267, 337, 344 , 351, 358, 428, 435 , 442, 449, 92, 99, 106 183, 190 , 197 274, 281 , 288 365, 372 , 379 456, 463 , 470 843 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 299 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F1 (CONTINUED FROM PREVIOUS PAGE) C92913 T 105 NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) VENTRAL-ABDOMINAL WEEK 91 C92914 1 C92915 M 4 NORMAL NO REMARKABLE OBSERVATIONS 96 BEHAVIOR HYPOACTIVE <UNSPECIFIED> EXCRETION FEW FECES NO FECES EYES RED DISCHARGE EYES SKIN & PELAGE SORE/SCAB PAWS-HIND NORMAL NO REMARKABLE OBSERVATIONS DOSE: 0 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 477, 484, 491, 498, 505, 512, 519, 526, 533, 540, 547, 554 568, 575, 582, 589, 596, 603, 610, 617, 624 631, 638, 645C, 652C, 659C, 666C, 673C, 680C, 687C, 694C, 701C 708C, 715C, 722C, 729C, 731C 1, 8 , 15, 22 670 645, 659 670 670 652, 659, 666 , 670 1 , 8 ,r 15, 2 2 , 29, 36, 43,, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127,. 134, 141, 148, 155,. 162, 169, 176, 183,. 190, 197 204, 2 1 1 , 218,. 225, 232, 239, 246,. 253, 260, 267, 274, 281, 288 844 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 300 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F1 (CONTINUED FROM PREVIOUS PAGE) C92915 M 96 NORMAL NO REMARKABLE OBSERVATIONS C92916 M MASS(ES) AXILLARY REGION-LEFT WEEK 80 93 APPEARANCE HEAD TILT RIGHT MALOCCLUSION EXCRETION FEW FECES EYES PROTRUDING EYES SKIN & PELAGE SORE/SCAB DORSAL-THORACIC-RIGHT LATERAL-THORACIC-LEFT PAW-HIND-RIGHT SHOULDER-LEFT NORMAL NO REMARKABLE OBSERVATIONS DOSE: 0 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470 477, 484, 491, 498, 505, 512, 519, 526, 533, 540, 547, 575, 582 589, 596, 603, 610, 617, 624, 631, 638 554, 561, 568 617, 624, 631, 638, 645, 649 22 624, 631, 638 519, 526, 533 519, 610, 526, 589, 526, 526, 617, 533, 596, 533, 533, 624 540, 603, 540, 540, 547, 610, 547, 547, 554, 617, 554, 554, 561, 624, 561, 561, 568, 631, 568, 568, 575, 638, 575, 575, 582, 645, 582, 582, 589, 649 589, 589, 596 596, 596, 603 603 1, 8,r 15, 29, 36, 43 , 50,r 57, 64, 71, 78 , 85,r 92, 99, 106, 113 845 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 301 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F1 (CONTINUED FROM PREVIOUS PAGE) C92916 M 93 NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) AXILLARY REGION-LEFT WEEK 48 WEEK 70 AXILLARY REGION-RIGHT WEEK 46 INGUINAL REGION-LEFT WEEK 84 INGUINAL REGION-RIGHT WEEK 75 VENTRAL-ABDOMINAL-RIGHT WEEK 38 WART LIKE LESION(S) MOUTH WEEK 75 DOSE: 0 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 120, 127, 134, 141, 148,, 155, 162, 169, 176, 183 , 190, 197, 204 2 1 1 , 218, 225, 232, 239,, 246, 253 330 484, 491, 498, 505, 512, 519, 526C, 533C , 540C, 54 7C, 554C, 561C 568C, 575C , 582C, 589C, 596C, 603C, 610C , 617C, 624C, 631C, 638C 645C, 649C 316C, 393C, 470C, 54 7C, 624C, 323C , 330C, 400C , 407C, 477C , 484C, 554C , 561C, 631C , 638C, 337C, 414C, 491C, 568C, 645C, 344C, 421C, 498C, 575C, 649C 351C, 428C, 505C, 582C, 358C , 365C, 435C , 442C, 512C , 519C, 589C , 596C, 372C, 44 9C, 526C, 603C, 379C, 456C, 533C, 610C, 386C 463C 540C 617C 582C, 589C , 596C, 603C, 610C, 617C, 624C , 631C, 638C, 645C, 649C 519C, 526C , 533C, 540C, 54 7C, 554C, 561C , 568C, 575C, 582C, 589C 596C, 603C , 610C, 617C, 624C, 631C, 638C , 645C, 649C 260, 267, 274, 281, 288, 295, 302, 309, 316 519, 526, 533, 540, 547,, 554, 561, 568, 575, 582 , 589,r 596, 603 610, 617, 624, 631, 638, 645, 649 846 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 302 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F1 DOSE: 0 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92917 3 53 NORMAL NO REMARKABLE OBSERVATIONS M 73 APPEARANCE THIN BEHAVIOR HYPOACTIVE <UNSPECIFIED> EXCRETION FEW FECES EYES RED DISCHARGE EYES SKIN & PELAGE SORE/SCAB VENTRAL-ABDOMINAL-RIGHT NORMAL NO REMARKABLE OBSERVATIONS 1 , 8 , 15,. 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 ,. 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365- 366 508 508 504- 505, 508 504- 505, 508 379 1 , 8 , 15,. 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0, 127, 134 , 141, 148, 155, 162 , 197, 204, 2 1 1 , 218 , 225 232, 239,. 246, 253 , 260, 267, 274, 281 , 288, 295, 302, 309 , 316 323, 330, 337, 344 , 351, 358, 365, 372 , 386, 393, 400, 407 , 414 421, 428, 435, 442 , 449, 456, 463, 470 , 477, 484, 491, 498 847 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 303 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F1 (CONTINUED FROM PREVIOUS PAGE) C92918 M 73 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 25 C92919 1 4 NORMAL NO REMARKABLE OBSERVATIONS C92920 M 72 APPEARANCE HUNCHED EXCRETION FEW FECES NO FECES NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) VENTRAL-ABDOMINAL WEEK 36 VENTRAL-ABDOMINAL-LEFT WEEK 34 DOSE: 0 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 169, 176, 183, 190 1, 8 , 15, 22 498 489, 491 498 1 , 8 , 15 113, 120 204, 211 351, 358 442, 449 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 218, 225 , 288, 295, 302, 309 , 316, 323, 330, 337 , 344 365, 372 , 379, 386, 393, 400 , 407, 414, 421, 428 , 435 456, 463 , 470, 477, 484 246C, 253C, 260C, 267, 274, 281 232C, 239C, 246C, 253C, 260C, 267C, 274C, 281C 848 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 304 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F1 DOSE: 0 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92921 M 57 APPEARANCE THIN BEHAVIOR HYPOACTIVE <UNSPECIFIED> EXCRETION FEW FECES EYES RED DISCHARGE EYES RESPIRATION LABORED SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE ROUGH HAIR COAT SORE/SCAB AXILLARY REGION-LEFT NORMAL NO REMARKABLE OBSERVATIONS 372, 379, 386, 393 379, 386, 393 337, 344, 351,. 358, 365, 372, 379, 386 , 393 372, 379, 386, 393 393 365, 372, 379,. 386, 393 365, 372, 379,. 386, 393 379, 386, 393 1 , 8 ,r 15, 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0, 127,. 134, 141, 148, 155, 162 , 169, 176, 183,. 190, 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281, 288 323 849 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 305 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F1 (CONTINUED FROM PREVIOUS PAGE) C92921 M 57 MASS(ES) AXILLARY REGION-LEFT WEEK 48 HEAD-MAXILLARY-RIGHT WEEK 43 WART LIKE LESION(S) MOUTH WEEK 46 C92922 3 53 NORMAL NO REMARKABLE OBSERVATIONS 3 53 SKIN & PELAGE SORE/SCAB DORSAL-THORACIC-RIGHT NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 48 DOSE: 0 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 330, 337C, 344C, 351C, 358C, 365C, 372C, 379C, 386C, 393C 295, 302, 309, 316 316 1 , 8 ,r 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190, 197 204, 2 1 1 , 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281, 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365-366 1 , 8 ,r 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0, 127, 134 , 141, 148, 155,. 162, 169, 176, 183,. 190, 197 204, 2 1 1 , 218, 225 , 232, 239, 246,. 253, 260, 267, 274, 281, 288 295, 302, 309, 316 , 323, 351, 358 330, 337, 344 850 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 306 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F1 (CONTINUED FROM PREVIOUS PAGE) C92923 3 53 SURGERY PERFORMED BY PATTIE MURPHY ANTIBIOTIC ADMINISTERED 0.5 ML C92924 M 85 NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) LIMB-HIND-LEFT WEEK 83 VENTRAL-ABDOMINAL WEEK 30 T 105 EYES CLEAR DISCHARGE EYE-RIGHT RED DISCHARGE EYE-RIGHT SKIN & PELAGE SORE/SCAB VENTRAL-ABDOMINAL DOSE: 0 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 359 359-360 1 , 8 , 15, 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190, 197 232, 239, 246,. 253, 260, 267, 274, 281 , 288, 295, 302, 309, 316 323, 330, 337,. 344, 351, 358, 365, 372 , 379, 386, 393, 400, 407 414, 421, 428, 435, 442, 449, 456, 463 , 470, 477, 484, 491, 498 505, 512, 519,. 526, 533, 540, 547, 554 , 561, 568 575C, 582C , 589C 204, 2 1 1 , 218, 225 526 533, 540, 547,. 554, 561, 568, 575, 582 , 589, 596, 603, 610, 617 624, 631, 638, 645, 652, 659, 666, 673 , 680, 687 722, 729, 733 851 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 307 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F1 (CONTINUED FROM PREVIOUS PAGE) C92925 T 105 NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) AXILLARY REGION-RIGHT WEEK 96 WEEK 99 INGUINAL REGION-RIGHT WEEK 84 VENTRAL-ABDOMINAL WEEK 92 C92926 1 C92927 T 4 NORMAL NO REMARKABLE OBSERVATIONS 105 EYES PROTRUDING EYES SKIN & PELAGE BLACK SKIN VENTRAL-THORACIC ROUGH HAIR COAT DOSE: 0 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 1 , 8 ,. 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43 , 50, 127, 134, 141, 148, 218, 225, 232, 239, 309, 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 57, 64, 71 , 78, 85 , 92, 99, 106 155, 162, 169, 176 , 183 , 190 , 197 246, 253, 260, 267 , 274 , 281 , 288 337, 344, 351, 358 , 365 , 372 , 379 428, 435, 442, 449 , 456 , 463 , 470 519 666 687C,. 694C, 701C, 708C, 715C, 722C , 72 9C , 733C 582C,. 589C, 596C, 603C, 610C, 617C , 624C , 631C, 638C, 645C , 652C 659C, 666C, 673C, 680C, 687C, 694C , 701C , 708C, 715C, 722C , 72 9C 733C 638, 645C, 652C, 659C, 666C, 673C, 680C, 687C, 694C, 701C, 708C 715C,. 722C, 729C, 733C 1 , 8 , 15, 22 519, 526, 533 729, 731 554 852 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 308 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F1 (CONTINUED FROM PREVIOUS PAGE) C92927 T 105 SKIN & PELAGE SORE/SCAB DORSAL DORSAL-THORACIC NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) AXILLARY REGION-RIGHT WEEK 88 INGUINAL REGION-LEFT WEEK 105 INGUINAL REGION-RIGHT WEEK 94 VENTRAL-CERVICAL WEEK 63 WEEK 82 VENTRAL-THORACIC-RIGHT WEEK 91 DOSE: 0 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 631, 638, 645,. 652, 659, 666, 673, 680, 687, 694, 701, 708 596 1 , 8 ,r 15, 2 2 , 29, 36 , 43,, 50, 113, 1 2 0, 127,. 134, 141, 148, 204, 2 1 1 , 218, 225, 232, 239, 295, 302, 309,. 316, 323, 330, 386, 393, 400, 407, 414, 421, 512, 540, 547, 561 57, 64, 71, 78 , 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 470, 477, 484, 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 491, 498, 505 610, 617, 624, 631, 638C, 645C , 652C, 659C, 666C, 673C , 680C 687C, 694C , 701C, 708C , 715C, 722C , 72 9C , 731C 72 9C, 731C 652, 659, 666, 673 , 680C, 687C , 694C, 701C, 708C, 715C, 722C 72 9C, 731C 435C, 442C , 449C, 456C , 463C 568, 575, 582C, 589C, 596C, 603C, 610C, 617C, 624C , 631C, 638C 645C, 652C , 659C, 666C , 673C, 680C , 687C , 694C, 70 1C, 708C, 715C 631C, 638C , 645C, 652C , 659C, 666C , 673C , 680C, 68 7C, 694C, 701C 708C, 715C , 722C, 72 9C , 731C 853 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 309 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F1 DOSE: 0 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92928 M 83 BEHAVIOR ATAXIC <UNSPECIFIED> HYPOACTIVE <UNSPECIFIED> EXCRETION FEW FECES NO FECES EYES RED DISCHARGE EYE-RIGHT EYES NORMAL NO REMARKABLE OBSERVATIONS C92929 M 60 APPEARANCE HEAD TILT LEFT MALOCCLUSION THIN BEHAVIOR CIRCLING RIGHT 575 575 575 572 505, 512, 519,. 533, 540, 547, 554, 561 526, 568, 572, 575 1 , 8 ,r 15, 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498 344, 351, 358, 365, 372, 379, 386, 393 , 400, 407, 414415 351, 358, 365,. 372, 379, 386, 393, 400 , 407, 414- 415 372, 379, 386, 393, 400, 407, 414- 415 358 854 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 310 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F1 (CONTINUED FROM PREVIOUS PAGE) C92929 M 60 EXCRETION FEW FECES NORMAL NO REMARKABLE OBSERVATIONS 3 53 APPEARANCE MALOCCLUSION SKIN & PELAGE SORE/SCAB DORSAL-THORACIC-RIGHT NORMAL NO REMARKABLE OBSERVATIONS SURGERY PERFORMED BY JOHN VAN BERKUM ANTIBIOTIC ADMINISTERED 0.5 ML DOSE: 0 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 337 1 , 8 , 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0, 127, 134 , 141, 148, 155, 162 , 169, 176, 183,. 190, 197 204, 2 1 1 , 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281, 288 295, 302, 309, 316 , 323, 330 176, 183 365- 366 1 , 8 , 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0, 127, 134 , 141, 148, 155, 162 , 169, 190, 197, 204, 211 218, 225, 232, 239 , 246, 253, 260, 267 , 274, 281, 288, 295, 302 309, 316, 323, 330 , 337, 344, 351, 358 359 359-360 855 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS CATEGORY KEYWORD WK QUALIFIER FREE-TEXT COMMENT 41 MASS(ES) 47 VENTRAL-ABDOMINAL WEEK 41 104 MASS(ES) VENTRAL-ABDOMINAL WEEK 104 105 MASS(ES) 105 VENTRAL-ABDOMINAL WEEK 104 94 MASS(ES) 98 INGUINAL REGION-LEFT WEEK 94 99 MASS(ES) 100 INGUINAL REGION-LEFT WEEK 94 101 MASS(ES) 103 INGUINAL REGION-LEFT WEEK 94 104 MASS(ES) 105 INGUINAL REGION-LEFT WEEK 94 76 MASS(ES) 78 VENTRAL-THORACIC-LEFT WEEK 76 15 X 15MM 10 X 10MM 15 X 15MM 40 X 40MM 50 X 40MM 40 X 40MM 50 X 50MM 25 X 20MM 856 Covance 6329-183 3M T-6295 PAGE: 311 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C92871 C92872 F1 547 79 TO 582 84 MASS(ES) VENTRAL-THORACIC-LEFT WEEK 76 589 85 MASS(ES) VENTRAL-THORACIC-LEFT WEEK 76 596 86 TO 659 95 MASS(ES) VENTRAL-THORACIC-LEFT WEEK 76 666 96 MASS(ES) VENTRAL-THORACIC-LEFT WEEK 76 673 97 MASS(ES) VENTRAL-THORACIC-LEFT WEEK 76 680 98 MASS(ES) TO 694 100 VENTRAL-THORACIC-LEFT WEEK 76 701 101 MASS(ES) TO 731 105 VENTRAL-THORACIC-LEFT WEEK 76 F1 596 86 TO 610 88 MASS(ES) INGUINAL REGION-LEFT WEEK 83 30 X 40MM 40 X 50MM 35 X 40MM 45 X 50MM 50 X 50MM 60 X 50MM 50 X 50MM 10 X 10MM 857 Covance 6329-183 3M T-6295 PAGE: 312 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 89 MASS(ES) 89 INGUINAL REGION-LEFT WEEK 83 47 MASS(ES) 48 VENTRAL-ABDOMINAL WEEK 41 49 MASS(ES) VENTRAL-ABDOMINAL WEEK 41 50 MASS(ES) VENTRAL-ABDOMINAL WEEK 41 51 MASS(ES) VENTRAL-ABDOMINAL WEEK 41 52 MASS(ES) VENTRAL-ABDOMINAL WEEK 41 53 MASS(ES) 57 VENTRAL-ABDOMINAL WEEK 41 58 MASS(ES) 60 VENTRAL-ABDOMINAL WEEK 41 30 X 20MM 25 X 20MM 30 X 25MM 30 X 25MM 30 X 30MM 35 X 30MM 40 X 35MM 55 X 45MM 858 Covance 6329-183 3M T-6295 PAGE: 313 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 61 MASS(ES) 63 VENTRAL-ABDOMINAL WEEK 41 64 MASS(ES) VENTRAL-ABDOMINAL WEEK 41 65 MASS(ES) 68 VENTRAL-ABDOMINAL WEEK 41 69 MASS(ES) 73 VENTRAL-ABDOMINAL WEEK 41 74 MASS(ES) 78 VENTRAL-ABDOMINAL WEEK 41 79 MASS(ES) 80 VENTRAL-ABDOMINAL WEEK 41 81 MASS(ES) 89 VENTRAL-ABDOMINAL WEEK 41 79 MASS(ES) 82 AXILLARY REGION-LEFT WEEK 79 55 X 50MM 55 X 60MM 70 X 65MM 60 X 65MM 80 X 70MM 70 X 70MM 80 X 70MM 10 X 10MM 859 Covance 6329-183 3M T-6295 PAGE: 314 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE- TEXT C92874 C92876 C92878 F1 652 94 TO 659 95 MASS(ES) VENTRAL-ABDOMINAL WEEK 93 666 96 TO 673 97 MASS(ES) VENTRAL-ABDOMINAL WEEK 93 680 98 MASS(ES) TO 701 101 VENTRAL-ABDOMINAL WEEK 93 708 102 MASS(ES) TO 715 103 VENTRAL-ABDOMINAL WEEK 93 722 104 MASS(ES) TO 731 105 VENTRAL-ABDOMINAL WEEK 93 F1 365 53 TO 366 53 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 53 F1 414 60 TO 421 61 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 60 428 62 TO 463 67 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 60 10 X 10MM 15 X 15MM 20 X 20MM 25 X 25MM 40 X 30MM 10 X 10MM 10 X 10MM 15 X 15MM 860 Covance 6329-183 3M T-6295 PAGE: 315 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 68 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 60 69 MASS(ES) 71 VENTRAL-ABDOMINAL-LEFT WEEK 60 72 MASS(ES) 73 VENTRAL-ABDOMINAL-LEFT WEEK 60 74 MASS(ES) 75 VENTRAL-ABDOMINAL-LEFT WEEK 60 76 MASS(ES) 80 VENTRAL-ABDOMINAL-LEFT WEEK 60 81 MASS(ES) 81 VENTRAL-ABDOMINAL-LEFT WEEK 60 45 MASS(ES) VENTRAL-THORACIC WEEK 45 46 MASS(ES) 48 VENTRAL-THORACIC WEEK 45 30 X 30MM 20 X 20MM 30 X 20MM 35 X 30MM 35 X 35MM 35 X 35MM 20 X 20MM 30 X 30MM 861 Covance 6329-183 3M T-6295 PAGE: 316 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C92878 F1 337 49 TO 351 51 358 52 TO 393 57 400 58 TO 421 61 428 62 TO 442 64 449 65 TO 505 73 512 74 TO 519 75 526 76 TO 533 77 540 78 TO 554 80 MASS(ES) VENTRAL-THORACIC WEEK 45 MASS(ES) VENTRAL-THORACIC WEEK 45 MASS(ES) VENTRAL-THORACIC WEEK 45 MASS(ES) VENTRAL-THORACIC WEEK 45 MASS(ES) VENTRAL-THORACIC WEEK 45 MASS(ES) VENTRAL-THORACIC WEEK 45 MASS(ES) VENTRAL-THORACIC WEEK 45 MASS(ES) VENTRAL-THORACIC WEEK 45 35 X 30MM 45 X 40MM 55 X 60MM 60 X 60MM 75 X 70MM 90 X 80MM 100 X 85MM 90 X 80MM 862 Covance 6329-183 3M T-6295 PAGE: 317 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 81 MASS(ES) 81 VENTRAL-THORACIC WEEK 45 69 MASS(ES) 73 AXILLARY REGION-LEFT WEEK 69 74 MASS(ES) 86 AXILLARY REGION-LEFT WEEK 69 87 MASS(ES) 93 AXILLARY REGION-LEFT WEEK 69 65 MASS(ES) 73 INGUINAL REGION-LEFT WEEK 65 74 MASS(ES) 80 INGUINAL REGION-LEFT WEEK 65 50 MASS(ES) INGUINAL REGION-RIGHT WEEK 50 69 MASS(ES) 71 VENTRAL-ABDOMINAL-RIGHT WEEK 65 100 X 100MM 10 X 10MM 15 X 15MM 10 X 10MM 10 X 10MM 15 X 15MM 10 X 10MM 10 X 10MM 863 Covance 6329-183 3M T-6295 PAGE: 318 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C92879 C92881 C92882 F1 638 92 TO 645 93 MASS(ES) RECTUM WEEK 92 F1 687 99 MASS(ES) TO 722 104 INGUINAL REGION-LEFT WEEK 99 729 105 MASS(ES) TO 733 105 INGUINAL REGION-LEFT WEEK 99 F1 477 69 MASS(ES) AXILLARY REGION-LEFT WEEK 69 484 70 TO 491 71 MASS(ES) AXILLARY REGION-LEFT WEEK 69 498 72 TO 505 73 MASS(ES) AXILLARY REGION-LEFT WEEK 69 512 74 MASS(ES) AXILLARY REGION-LEFT WEEK 69 519 75 MASS(ES) AXILLARY REGION-LEFT WEEK 69 15 X 15MM 10 X 10MM 20 X 20MM 20 X 20MM 20 X 25MM 30 X 30MM 30 X 40MM 45 X 40MM 864 Covance 6329-183 3M T-6295 PAGE: 319 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 76 MASS(ES) 79 AXILLARY REGION-LEFT WEEK 69 80 MASS(ES) AXILLARY REGION-LEFT WEEK 69 81 MASS(ES) 85 AXILLARY REGION-LEFT WEEK 69 86 MASS(ES) AXILLARY REGION-LEFT WEEK 69 87 MASS(ES) 88 AXILLARY REGION-LEFT WEEK 69 89 MASS(ES) AXILLARY REGION-LEFT WEEK 69 90 MASS(ES) AXILLARY REGION-LEFT WEEK 69 91 MASS(ES) AXILLARY REGION-LEFT WEEK 69 50 X 40MM 50 X 50MM 50 X 60MM 60 X 65MM 60 X 50MM 75 X 60MM 75 X 60MM 75 X 60MM 865 Covance 6329-183 3M T-6295 PAGE: 320 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C92882 C92883 C92884 F1 638 92 MASS(ES) AXILLARY REGION-LEFT WEEK 69 645 93 TO 659 95 MASS(ES) AXILLARY REGION-LEFT WEEK 69 666 96 MASS(ES) TO 733 105 AXILLARY REGION-LEFT WEEK 69 694 100 MASS(ES) TO 733 105 AXILLARY REGION-RIGHT WEEK 100 F1 519 75 TO 575 83 MASS(ES) INGUINAL REGION-RIGHT WEEK 75 582 84 MASS(ES) INGUINAL REGION-RIGHT WEEK 75 589 85 TO 604 87 MASS(ES) INGUINAL REGION-RIGHT WEEK 75 F1 505 73 MASS(ES) AXILLARY REGION-LEFT WEEK 73 75 X 60MM 70 X 65MM 80 X 65MM 10 X 10MM 10 X 10MM 15 X 15MM 20 X 20MM 10 X 10MM 866 Covance 6329-183 3M T-6295 PAGE: 321 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 89 MASS(ES) 93 AXILLARY REGION-LEFT WEEK 89 94 MASS(ES) AXILLARY REGION-LEFT WEEK 89 95 MASS(ES) AXILLARY REGION-LEFT WEEK 89 96 MASS(ES) 96 AXILLARY REGION-LEFT WEEK 89 85 MASS(ES) 86 AXILLARY REGION-RIGHT WEEK 85 89 MASS(ES) 90 VENTRAL-ABDOMINAL-LEFT WEEK 79 91 MASS(ES) 95 VENTRAL-ABDOMINAL-LEFT WEEK 79 96 MASS(ES) 96 VENTRAL-ABDOMINAL-LEFT WEEK 79 15 X 15MM 25 X 20MM 15 X 15MM 20 X 15MM 10 X 10MM 15 X 15MM 20 X 20MM 30 X 20MM 867 Covance 6329-183 3M T-6295 PAGE: 322 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT F1 449 65 TO 498 72 505 73 TO 519 75 526 76 TO 547 79 554 80 561 81 TO 666 96 512 74 519 75 TO 540 78 547 79 TO 582 84 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 65 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 65 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 65 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 65 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 65 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 74 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 74 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 74 10 X10MM 15 X 15MM 15 X 20MM 20 X 25MM 25 X 30MM 30 X 20MM POSTERIOR 20 X 15MM POSTERIOR 20 X 20MM POSTERIOR 868 Covance 6329-183 3M T-6295 PAGE: 323 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C92884 C92885 C92886 F1 589 85 TO 596 86 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 74 603 87 TO 610 CO CO MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 74 617 89 TO 659 95 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 74 666 96 TO 666 96 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 74 F1 708 102 MASS(ES) TO 715 103 INGUINAL REGION-LEFT WEEK 99 722 104 MASS(ES) TO 733 105 INGUINAL REGION-LEFT WEEK 99 F1 421 61 TO 428 62 MASS(ES) AXILLARY REGION-LEFT WEEK 59 435 63 TO 442 64 MASS(ES) AXILLARY REGION-LEFT WEEK 59 30 X 30MM POSTERIOR 20 X 20MM POSTERIOR 25 X 25MM POSTERIOR 30 X 25MM POSTERIOR 10 X 10MM 15 X 15MM 15 X 15MM 20 X 15MM 869 Covance 6329-183 3M T-6295 PAGE: 324 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C92886 C92893 F1 449 65 TO 470 68 477 69 TO 533 77 407 59 TO 463 67 F1 519 75 TO 540 78 561 81 TO 610 526 76 TO 554 80 526 76 TO 568 82 519 75 CO CO MASS(ES) AXILLARY REGION-LEFT WEEK 59 MASS(ES) AXILLARY REGION-LEFT WEEK 59 MASS(ES) INGUINAL REGION-RIGHT WEEK 59 MASS(ES) AXILLARY REGION-RIGHT WEEK 74 MASS(ES) AXILLARY REGION-RIGHT WEEK 74 MASS(ES) INGUINAL REGION-RIGHT WEEK 76 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 76 MASS(ES) VENTRAL-THORACIC-LEFT WEEK 74 25 X 20MM 10 X 10MM 15 X 15MM 10 X 10MM 10 X 10MM 10 X 10MM 10 X 10MM 10 X 10MM 870 Covance 6329-183 3M T-6295 PAGE: 325 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 76 MASS(ES) 78 VENTRAL-THORACIC-LEFT WEEK 74 79 MASS(ES) VENTRAL-THORACIC-LEFT WEEK 74 80 MASS(ES) VENTRAL-THORACIC-LEFT WEEK 74 81 MASS(ES) 83 VENTRAL-THORACIC-LEFT WEEK 74 84 MASS(ES) VENTRAL-THORACIC-LEFT WEEK 74 85 MASS(ES) VENTRAL-THORACIC-LEFT WEEK 74 86 MASS(ES) VENTRAL-THORACIC-LEFT WEEK 74 87 MASS(ES) 88 VENTRAL-THORACIC-LEFT WEEK 74 15 X 15MM 10 X 10MM 15 X 15MM 25 X 25MM 30 X 30MM 40 X 40MM 40 X 45MM 40 X 40MM 871 Covance 6329-183 3M T-6295 PAGE: 326 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 61 MASS(ES) RECTUM WEEK 61 62 MASS(ES) RECTUM WEEK 61 63 MASS(ES) 67 RECTUM WEEK 61 87 MASS(ES) 91 AXILLARY REGION-LEFT WEEK 87 92 MASS(ES) 93 AXILLARY REGION-LEFT WEEK 87 94 MASS(ES) AXILLARY REGION-LEFT WEEK 87 95 MASS(ES) AXILLARY REGION-LEFT WEEK 87 96 MASS(ES) 97 AXILLARY REGION-LEFT WEEK 87 15 X 20MM 15 X 20MM 15 X 20MM 10 X 10MM 15 X 15MM 20 X 15MM 25 X 25MM 30 X 25MM 872 Covance 6329-183 3M T-6295 PAGE: 327 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C92895 C92896 F1 680 98 MASS(ES) TO 701 101 AXILLARY REGION-LEFT WEEK 87 708 102 MASS(ES) TO 732 105 AXILLARY REGION-LEFT WEEK 87 F1 526 76 TO 554 80 MASS(ES) INGUINAL REGION-RIGHT WEEK 76 561 81 TO 589 85 MASS(ES) INGUINAL REGION-RIGHT WEEK 76 596 86 TO 610 CO CO MASS(ES) INGUINAL REGION-RIGHT WEEK 76 617 89 TO 624 90 MASS(ES) INGUINAL REGION-RIGHT WEEK 76 631 91 MASS(ES) TO 715 103 INGUINAL REGION-RIGHT WEEK 76 722 104 MASS(ES) TO 731 105 INGUINAL REGION-RIGHT WEEK 76 40 X 30MM 45 X 40MM 20 X 15MM 25 X 20MM 30 X 25MM 35 X 30MM 50 X 35MM 60 X 40MM 873 Covance 6329-183 3M T-6295 PAGE: 328 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C92896 C92901 C92904 F1 673 97 MASS(ES) TO 701 101 VENTRAL-ABDOMINAL-RIGHT WEEK 97 708 102 MASS(ES) TO 715 103 VENTRAL-ABDOMINAL-RIGHT WEEK 97 F1 526 76 TO 551 79 MASS(ES) RECTUM WEEK 76 F1 484 70 TO 498 72 MASS(ES) AXILLARY REGION-LEFT WEEK 69 505 73 MASS(ES) AXILLARY REGION-LEFT WEEK 69 512 74 TO 540 78 MASS(ES) AXILLARY REGION-LEFT WEEK 69 547 79 TO 554 80 MASS(ES) AXILLARY REGION-LEFT WEEK 69 561 81 TO 582 84 MASS(ES) AXILLARY REGION-LEFT WEEK 69 20 X 15MM 30 X 30MM 10 X 10MM 10 X 10MM 15 X 15MM 30 X 25MM 30 X 30MM 35 X 35MM 874 Covance 6329-183 3M T-6295 PAGE: 329 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C92904 F1 589 85 MASS(ES) AXILLARY REGION-LEFT WEEK 69 596 86 TO 603 87 MASS(ES) AXILLARY REGION-LEFT WEEK 69 610 88 MASS(ES) AXILLARY REGION-LEFT WEEK 69 617 89 TO 638 92 MASS(ES) AXILLARY REGION-LEFT WEEK 69 645 93 TO 659 95 MASS(ES) AXILLARY REGION-LEFT WEEK 69 666 96 TO 673 97 MASS(ES) AXILLARY REGION-LEFT WEEK 69 680 98 MASS(ES) TO 702 101 AXILLARY REGION-LEFT WEEK 69 484 70 TO 512 74 MASS(ES) INGUINAL REGION-RIGHT WEEK 70 40 X 40MM 35 X 45MM 40 X 45MM 50 X 50MM 50 X 55MM 50 X 60MM 55 X 65MM 10 X 10MM 875 Covance 6329-183 3M T-6295 PAGE: 330 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS CATEGORY KEYWORD WK QUALIFIER FREE-TEXT COMMENT 75 MASS(ES) INGUINAL REGION-RIGHT WEEK 70 76 MASS(ES) INGUINAL REGION-RIGHT WEEK 70 77 MASS(ES) INGUINAL REGION-RIGHT WEEK 70 78 MASS(ES) INGUINAL REGION-RIGHT WEEK 70 79 MASS(ES) INGUINAL REGION-RIGHT WEEK 70 80 MASS(ES) INGUINAL REGION-RIGHT WEEK 70 81 MASS(ES) INGUINAL REGION-RIGHT WEEK 70 82 MASS(ES) INGUINAL REGION-RIGHT WEEK 70 15 X 10MM 20 X 10MM 20 X 10MM 20 X 10MM 20 X 15MM 20 X 15MM 20 X 20MM 20 X 20MM 876 Covance 6329-183 3M T-6295 PAGE: 331 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C92904 F1 575 83 582 84 589 85 596 86 603 87 610 88 617 89 624 90 MASS(ES) INGUINAL REGION-RIGHT WEEK 70 MASS(ES) INGUINAL REGION-RIGHT WEEK 70 MASS(ES) INGUINAL REGION-RIGHT WEEK 70 MASS(ES) INGUINAL REGION-RIGHT WEEK 70 MASS(ES) INGUINAL REGION-RIGHT WEEK 70 MASS(ES) INGUINAL REGION-RIGHT WEEK 70 MASS(ES) INGUINAL REGION-RIGHT WEEK 70 MASS(ES) INGUINAL REGION-RIGHT WEEK 70 20 X 20MM 20 X 20MM 20 X 20MM 20 X 20MM 35 X 30MM 35 X 30MM 40 X 35MM 40 X 35MM 877 Covance 6329-183 3M T-6295 PAGE: 332 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C92904 F1 631 91 638 92 645 93 652 94 659 95 666 96 673 97 680 98 MASS(ES) INGUINAL REGION-RIGHT WEEK 70 MASS(ES) INGUINAL REGION-RIGHT WEEK 70 MASS(ES) INGUINAL REGION-RIGHT WEEK 70 MASS(ES) INGUINAL REGION-RIGHT WEEK 70 MASS(ES) INGUINAL REGION-RIGHT WEEK 70 MASS(ES) INGUINAL REGION-RIGHT WEEK 70 MASS(ES) INGUINAL REGION-RIGHT WEEK 70 MASS(ES) INGUINAL REGION-RIGHT WEEK 70 40 X 35MM 40 X 35MM 40 X 35MM 40 X 35MM 40 X 35MM 40 X 35MM 40 X 35MM 40 X 35MM 878 Covance 6329-183 3M T-6295 PAGE: 333 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C92904 F1 687 99 MASS(ES) INGUINAL REGION-RIGHT WEEK 70 694 100 MASS(ES) INGUINAL REGION-RIGHT WEEK 70 701 101 MASS(ES) INGUINAL REGION-RIGHT WEEK 70 702 101 MASS(ES) INGUINAL REGION-RIGHT WEEK 70 702 101 MASS(ES) INGUINAL REGION-RIGHT WEEK 70 673 97 MASS(ES) TO 702 101 VENTRAL-ABDOMINAL-LEFT WEEK 97 449 65 TO 463 67 MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 63 470 68 TO 498 72 MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 63 40 X 35MM 40 X 35MM 40 X 35MM 40 X 35MM 40 X 35MM 30 X 20MM 10 X 10MM 15 X 15MM 879 Covance 6329-183 3M T-6295 PAGE: 334 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 73 MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 63 74 MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 63 75 MASS(ES) 77 VENTRAL-CERVICAL-LEFT WEEK 63 78 MASS(ES) 86 VENTRAL-CERVICAL-LEFT WEEK 63 73 MASS(ES) VENTRAL-THORACIC-LEFT WEEK 73 74 MASS(ES) 78 VENTRAL-THORACIC-LEFT WEEK 73 79 MASS(ES) VENTRAL-THORACIC-LEFT WEEK 73 80 MASS(ES) 83 VENTRAL-THORACIC-LEFT WEEK 73 20 X 20MM 30 X 30MM 25 X 25MM 10 X 10MM 10 X 10MM 20 X 20MM 20 X 30MM 30 X 35MM 880 Covance 6329-183 3M T-6295 PAGE: 335 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS CATEGORY KEYWORD WK QUALIFIER FREE-TEXT COMMENT 84 MASS(ES) 85 VENTRAL-THORACIC-LEFT WEEK 73 86 MASS(ES) 90 VENTRAL-THORACIC-LEFT WEEK 73 91 MASS(ES) 101 VENTRAL-THORACIC-LEFT WEEK 73 95 MASS(ES) 103 AXILLARY REGION-LEFT WEEK 95 104 MASS(ES) 105 AXILLARY REGION-LEFT WEEK 95 95 MASS(ES) 98 AXILLARY REGION-RIGHT WEEK 95 99 MASS(ES) 102 AXILLARY REGION-RIGHT WEEK 95 103 MASS(ES) 105 AXILLARY REGION-RIGHT WEEK 95 40 X 40MM 30 X 25MM 30 X 35MM 15 X 15MM 20 X 20MM 20 X 20MM 30 X 25MM 40 X 30MM 881 Covance 6329-183 3M T-6295 PAGE: 336 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C92908 C92909 C92911 F1 540 78 TO 554 80 MASS(ES) VENTRAL-CERVICAL WEEK 78 561 81 TO 582 84 MASS(ES) VENTRAL-CERVICAL WEEK 78 589 85 TO 646 93 MASS(ES) VENTRAL-CERVICAL WEEK 78 F1 680 98 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 98 687 99 MASS(ES) TO 701 101 VENTRAL-ABDOMINAL-RIGHT WEEK 98 708 102 MASS(ES) TO 715 103 VENTRAL-ABDOMINAL-RIGHT WEEK 98 722 104 MASS(ES) TO 732 105 VENTRAL-ABDOMINAL-RIGHT WEEK 98 F1 477 69 MASS(ES) AXILLARY REGION-LEFT WEEK 69 10 X 10MM 15 X 15MM 20 X 20MM 20 X 20MM 30 X 20MM 45 X 30MM 80 X 45MM 10 X 10MM 882 Covance 6329-183 3M T-6295 PAGE: 337 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 70 MASS(ES) 72 AXILLARY REGION-LEFT WEEK 69 73 MASS(ES) AXILLARY REGION-LEFT WEEK 69 74 MASS(ES) 75 AXILLARY REGION-LEFT WEEK 69 76 MASS(ES) AXILLARY REGION-LEFT WEEK 69 81 MASS(ES) 82 VENTRAL-ABDOMINAL-LEFT WEEK 80 83 MASS(ES) 85 VENTRAL-ABDOMINAL-LEFT WEEK 80 86 MASS(ES) 88 VENTRAL-ABDOMINAL-LEFT WEEK 80 89 MASS(ES) 90 VENTRAL-ABDOMINAL-LEFT WEEK 80 15 X 15MM 10 X 10MM 20 X 20MM 15 X 15MM 10 X 10MM 30 X 20MM 30 X 30MM 40 X 45MM 883 Covance 6329-183 3M T-6295 PAGE: 338 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C92912 F1 631 91 TO 638 92 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 80 645 93 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 80 652 94 TO 659 95 666 96 TO 680 98 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 80 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 80 687 99 MASS(ES) TO 701 101 VENTRAL-ABDOMINAL-LEFT WEEK 80 708 102 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 80 715 103 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 80 722 104 MASS(ES) TO 732 105 VENTRAL-ABDOMINAL-LEFT WEEK 80 70 X 50MM 60 X 50MM 60 X 60MM 70 X 60MM 70 X 70MM 70 X 90MM 90 X 70MM 90 X 100MM 884 Covance 6329-183 3M T-6295 PAGE: 339 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS CATEGORY KEYWORD WK QUALIFIER FREE-TEXT COMMENT 93 MASS(ES) 94 VENTRAL-ABDOMINAL WEEK 91 95 MASS(ES) VENTRAL-ABDOMINAL WEEK 91 96 MASS(ES) 100 VENTRAL-ABDOMINAL WEEK 91 101 MASS(ES) VENTRAL-ABDOMINAL WEEK 91 102 MASS(ES) 103 VENTRAL-ABDOMINAL WEEK 91 104 MASS(ES) 105 VENTRAL-ABDOMINAL WEEK 91 76 MASS(ES) 93 AXILLARY REGION-LEFT WEEK 70 46 MASS(ES) AXILLARY REGION-RIGHT WEEK 46 10 X 10MM 15 X 15MM 25 X 20MM 30 X 30MM 40 X 40MM 50 X 45MM 10 X 10MM 20 X 20MM 885 Covance 6329-183 3M T-6295 PAGE: 340 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 47 MASS(ES) 48 AXILLARY REGION-RIGHT WEEK 46 49 MASS(ES) 59 AXILLARY REGION-RIGHT WEEK 46 60 MASS(ES) 64 AXILLARY REGION-RIGHT WEEK 46 65 MASS(ES) 74 AXILLARY REGION-RIGHT WEEK 46 75 MASS(ES) 79 AXILLARY REGION-RIGHT WEEK 46 80 MASS(ES) AXILLARY REGION-RIGHT WEEK 46 81 MASS(ES) 87 AXILLARY REGION-RIGHT WEEK 46 88 MASS(ES) 90 AXILLARY REGION-RIGHT WEEK 46 25 X 20MM 20 X 20MM 25 X 20MM 60 X 45MM 25 X 25MM 35 X 35MM 60 X 50MM 50 X 40MM 886 Covance 6329-183 3M T-6295 PAGE: 341 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 91 MASS(ES) 92 AXILLARY REGION-RIGHT WEEK 46 93 MASS(ES) 93 AXILLARY REGION-RIGHT WEEK 46 84 MASS(ES) 85 INGUINAL REGION-LEFT WEEK 84 86 MASS(ES) INGUINAL REGION-LEFT WEEK 84 87 MASS(ES) INGUINAL REGION-LEFT WEEK 84 88 MASS(ES) 90 INGUINAL REGION-LEFT WEEK 84 91 MASS(ES) INGUINAL REGION-LEFT WEEK 84 92 MASS(ES) 93 INGUINAL REGION-LEFT WEEK 84 30 X 20MM 15 X 15MM 15 X 15MM 20 X 20MM 15 X 15MM 20 X 20MM 10 X 10MM 20 X 20MM 887 Covance 6329-183 3M T-6295 PAGE: 342 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 75 MASS(ES) 83 INGUINAL REGION-RIGHT WEEK 75 84 MASS(ES) 86 INGUINAL REGION-RIGHT WEEK 75 87 MASS(ES) 88 INGUINAL REGION-RIGHT WEEK 75 89 MASS(ES) 93 INGUINAL REGION-RIGHT WEEK 75 36 MASS(ES) 38 VENTRAL-ABDOMINAL WEEK 36 34 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 34 35 MASS(ES) 41 VENTRAL-ABDOMINAL-LEFT WEEK 34 49 MASS(ES) 52 AXILLARY REGION-LEFT WEEK 48 10 X 10MM 15 X 15MM 20 X 20MM 50 X 40MM 10 X 10MM 25 X 20MM 30 X 35MM 10 X 10MM 888 Covance 6329-183 3M T-6295 PAGE: 343 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C92921 C92924 C92925 F1 365 53 TO 393 57 MASS(ES) AXILLARY REGION-LEFT WEEK 48 F1 575 83 MASS(ES) LIMB-HIND-LEFT WEEK 83 582 84 MASS(ES) LIMB-HIND-LEFT WEEK 83 589 85 TO 589 85 MASS(ES) LIMB-HIND-LEFT WEEK 83 F1 687 99 MASS(ES) AXILLARY REGION-RIGHT WEEK 99 694 100 MASS(ES) TO 715 103 AXILLARY REGION-RIGHT WEEK 99 722 104 MASS(ES) TO 733 105 AXILLARY REGION-RIGHT WEEK 99 582 84 TO 603 87 MASS(ES) INGUINAL REGION-RIGHT WEEK 84 15 X 15MM 45 X 40MM 60 X 50MM 80 X 60MM 10 X 10MM 15 X 15MM 20 X 20MM 40 X 30MM 889 Covance 6329-183 3M T-6295 PAGE: 344 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C92925 F1 610 88 MASS(ES) INGUINAL REGION-RIGHT WEEK 84 617 89 TO 624 90 MASS(ES) INGUINAL REGION-RIGHT WEEK 84 631 91 TO 659 95 MASS(ES) INGUINAL REGION-RIGHT WEEK 84 666 96 TO 680 98 MASS(ES) INGUINAL REGION-RIGHT WEEK 84 687 99 MASS(ES) TO 715 103 INGUINAL REGION-RIGHT WEEK 84 722 104 MASS(ES) TO 733 105 INGUINAL REGION-RIGHT WEEK 84 645 93 TO 680 98 MASS(ES) VENTRAL-ABDOMINAL WEEK 92 687 99 MASS(ES) TO 733 105 VENTRAL-ABDOMINAL WEEK 92 40 X 35MM 60 X 40MM 60 X 50MM 65 X 50MM 70 X 60MM 80 X 80MM 10 X 10MM 15 X 15MM 890 Covance 6329-183 3M T-6295 PAGE: 345 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT F1 638 92 TO 666 96 MASS(ES) AXILLARY REGION-RIGHT WEEK 88 673 97 MASS(ES) TO 715 103 AXILLARY REGION-RIGHT WEEK 88 722 104 MASS(ES) TO 731 105 AXILLARY REGION-RIGHT WEEK 88 729 105 MASS(ES) TO 731 105 INGUINAL REGION-LEFT WEEK 105 680 98 MASS(ES) TO 715 103 INGUINAL REGION-RIGHT WEEK 94 722 104 MASS(ES) TO 731 105 INGUINAL REGION-RIGHT WEEK 94 435 63 TO 463 67 MASS(ES) VENTRAL-CERVICAL WEEK 63 582 84 MASS(ES) VENTRAL-CERVICAL WEEK 82 10 X 10MM 20 X 20MM 40 X 40MM 15 X 15MM 15 X 10MM 25 X 20MM 10 X 10MM 10 X 10MM 891 Covance 6329-183 3M T-6295 PAGE: 346 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT F1 589 85 TO 659 95 MASS(ES) VENTRAL-CERVICAL WEEK 82 666 96 TO 673 97 MASS(ES) VENTRAL-CERVICAL WEEK 82 680 98 MASS(ES) TO 715 103 VENTRAL-CERVICAL WEEK 82 631 91 TO 645 93 MASS(ES) VENTRAL-THORACIC-RIGHT WEEK 91 652 94 TO 673 97 MASS(ES) VENTRAL-THORACIC-RIGHT WEEK 91 680 98 MASS(ES) TO 715 103 VENTRAL-THORACIC-RIGHT WEEK 91 722 104 MASS(ES) TO 731 105 VENTRAL-THORACIC-RIGHT WEEK 91 20 X 20MM 25 X 25MM 30 X 30MM 20 X 20MM 30 X 30MM 40 X 35MM 80 X 50MM MASSES APPEAR TO HAVE GROWN TOGETHER Covance 6329-183 3M T-6295 PAGE: 347 892 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 348 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F2 DOSE: 0.5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92931 D 70 EXCRETION FEW FECES NORMAL NO REMARKABLE OBSERVATIONS C92932 M MASS(ES) AXILLARY REGION-RIGHT WEEK 69 41 APPEARANCE THIN EXCRETION FEW FECES SKIN & PELAGE PALE ENTIRE BODY SORE/SCAB VENTRAL-ABDOMINAL-LEFT NORMAL NO REMARKABLE OBSERVATIONS 489-490 1 , 8 ,r 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407 , 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477C, 484C, 489-490C 281, 285 281, 285 281, 285 285 1, 8 , 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113 893 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 349 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F2 (CONTINUED FROM PREVIOUS PAGE) C92932 M 41 MASS(ES) DORSAL WEEK 29 VENTRAL-ABDOMINAL-LEFT WEEK 18 VENTRAL-ABDOMINAL-RIGHT WEEK 25 VENTRAL-THORACIC-LEFT WEEK 25 M 92 APPEARANCE MALOCCLUSION BEHAVIOR HYPOACTIVE <UNSPECIFIED> COLD TO TOUCH ENTIRE BODY EXCRETION FEW FECES NO FECES EYES PALE EYES DOSE: 0.5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 197C, 204C , 211C, 218C, 225C, 232C, 239C , 246C, 253C, 260C, 267C 274C 12 0C, 127C , 134C, 141C, 148C, 155C, 162C , 169C, 176C, 183C, 190C 197C, 204C , 211C, 218C, 225C, 232C, 239C , 246C, 253C, 260C, 267C 274C, 281C , 285C 16 9C, 176C , 183C, 190C, 197C, 204C, 211C , 218C, 225C, 232C, 239C 24 6C, 253C , 260C, 267C, 274C, 281C, 285C 16 9C, 176C , 183C, 190C 281, 316, 344, 358 , 407,, 414, 435, 442, 449, 456 , 463,r 470, 477 505, 512, 519, 540 , 547,, 554, 561, 568, 575, 582 , 589, 631, 638 642 642 642 642 642 642 894 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 350 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F2 (CONTINUED FROM PREVIOUS PAGE) C92933 M 92 EYES PROTRUDING EYES RESPIRATION LABORED SKIN & PELAGE BLACK SKIN VENTRAL-ABDOMINAL-RIGHT SORE/SCAB VENTRAL-ABDOMINAL-RIGHT NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) AXILLARY REGION-LEFT WEEK 64 INGUINAL REGION-RIGHT WEEK 74 DOSE: 0.5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 519 642 642 638, 642 1 , 8 ,. 15, 22, 29, 36, 43 , 50, 57, 64, 71 , 78, 85, 92, 99, 106 113, 1 2 0, 127, 134 , 141, 148, 155, 162, 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225 , 232, 239, 246, 253, 260, 267, 274, 288 , 295 302, 309, 323, 330 , 337, 351, 421, 428 442C, 449C , 456C, 463C, 470C, 477C , 484C , 491C, 498C, 505C , 512C 519C, 526C , 533C, 54 0C, 54 7C, 554C , 561C , 568C, 575C, 582C , 589C 596C, 603C , 610C, 617C, 624C, 631C , 638C , 642C 512C, 519C , 526C, 533C, 54 0C, 547C , 554C , 561C, 568C, 575C , 582C 58 9C, 596C , 603C, 610C, 617C, 624C , 631C , 638C, 642C 895 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 351 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F2 DOSE: 0.5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92934 M 87 APPEARANCE HEAD TILT RIGHT MALOCCLUSION MISSING TEETH THIN BEHAVIOR ATAXIC <UNSPECIFIED> EYES PROTRUDING EYES RESPIRATION LABORED NORMAL NO REMARKABLE OBSERVATIONS 596, 603 414, 421, 428, 435, 442, 449, 456, 463, 470, 477, 484, 491 505, 512, 519, 526, 533, 540, 547, 554, 561, 568, 575, 582 596, 603 575, 582, 589, 596, 603 596, 603 603 519 603 1 , 8 ,r 15, 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407 896 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 352 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F2 DOSE: 0.5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92935 M 88 APPEARANCE HUNCHED BEHAVIOR ATAXIC <UNSPECIFIED> EXCRETION FEW FECES EYES RED DISCHARGE EYE-RIGHT SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE ROUGH HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) AXILLARY REGION-RIGHT WEEK 81 SHOULDER-RIGHT WEEK 65 603, 610, 612 610, 612 575, 582, 610, 612 603, 610, 612 505 603 1 22, 8,r 15, , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 120113, , 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 211204, , 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407 , 414, 421, 428, 435 , 442, 470, 477, 484 , 491 498, 512, 519, 526 , 533, 540, 547, 554 561C, 568C, 575C, 582C, 589C, 596C, 603C 449C, 456C, 463C 897 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 353 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F2 DOSE: 0.5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS T 105 EXCRETION FEW FECES EYES RED DISCHARGE EYES NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) AXILLARY REGION-LEFT WEEK 63 C92937 M 65 APPEARANCE HUNCHED MALOCCLUSION THIN BEHAVIOR HYPOACTIVE <UNSPECIFIED> EXCRETION FEW FECES 722, 729, 732 722 1 , 8 , 15, 22, 29, 36, 43, 50, 57, 64, 71, 78,r 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281 , 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428 435C , 442C, 449C, 456C, 463C, 470C , 477C, 484C, 491C, 498C , 505C 512C , 519C, 526C, 533C, 540C, 547C , 554C, 561C, 568C, 575C , 582C 589C , 596C, 603C, 610C, 617C, 624C , 631C, 638C, 645C, 652C , 659C 666C , 673C, 680C, 687C, 694C, 701C , 708C, 715C, 722C, 72 9C , 732C 449- 450 400, 407, 414, 421, 428, 435, 442, 449-450 449- 450 450 393 898 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 354 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F2 (CONTINUED FROM PREVIOUS PAGE) C92937 M 65 NORMAL NO REMARKABLE OBSERVATIONS C92938 T MASS(ES) AXILLARY REGION-LEFT WEEK 64 VENTRAL-ABDOMINAL WEEK 61 VENTRAL-ABDOMINAL-LEFT WEEK 43 105 APPEARANCE SWOLLEN PAW-HIND-RIGHT SKIN & PELAGE SORE/SCAB PAW-HIND-RIGHT PAWS-HIND NORMAL NO REMARKABLE OBSERVATIONS DOSE: 0.5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 1 , 8 ,r 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127, 134 , 141, 148, 155, 162 , 169, 176, 183,. 190, 197 204, 2 1 1 , 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281, 288 323, 330, 337, 344 , 351, 358, 365, 372 , 379, 386 442, 449-450 421, 428C, 435C, 442C, 449-450C 295C, 302C, 309C, 316C 722, 729, 732 554, 561, 568,. 575, 582, 589, 596, 603 , 610, 617, 624, 631 , 638 645, 652, 659, 666 673, 680, 687,. 694, 701, 708, 715, 722 , 729, 732 1 , 8 ,r 15, 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547 899 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 355 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F2 DOSE: 0.5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92939 M 71 SKIN & PELAGE BLACK SKIN VENTRAL-CERVICAL-RIGHT SORE/SCAB VENTRAL-THORACIC NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) AXILLARY REGION-LEFT WEEK 58 VENTRAL-ABDOMINAL-RIGHT WEEK 63 VENTRAL-CERVICAL-RIGHT WEEK 47 C92940 M 87 APPEARANCE THIN NORMAL NO REMARKABLE OBSERVATIONS 484 491 1 , 8 ,r 15, 2 2 , 29, 36, 43,, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127,. 134, 141, 148, 155,. 162, 169, 176, 183,. 190, 197 204, 2 1 1 , 218,. 225, 232, 239, 246,. 253, 260, 267, 274, 281, 288 295, 302, 309, 316 400, 407, 414, 421 , 428C , 435C , 442C , 449C , 456C , 463C , 470C 477C, 484C , 491C 435C, 442C , 449C, 456C, 463C, 470C, 477C, 484C, 491C 323C, 330C , 337C, 344C, 351C, 358C, 365C, 372C, 379C, 386C, 393C 400C, 407C , 414C, 421C, 428C, 435C, 442C, 44 9C, 456C, 463C, 470C 477C, 484C , 491C 526 1 , 8 , 15 113, 120 204, 211 295, 302 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 127, 134 , 141, 148, 155, 162 , 169, 176, 183,. 190, 197 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281, 288 309, 316 , 323, 330, 337 900 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 356 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F2 (CONTINUED FROM PREVIOUS PAGE) C92940 M 87 MASS(ES) AXILLARY REGION-LEFT WEEK 50 C92941 1 C92942 M INGUINAL REGION-LEFT WEEK 73 VENTRAL-THORACIC-RIGHT WEEK 76 4 NORMAL NO REMARKABLE OBSERVATIONS 50 SKIN & PELAGE BLACK SKIN DORSAL SORE/SCAB DORSAL NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) DORSAL WEEK 11 VENTRAL-ABDOMINAL-RIGHT WEEK 50 DOSE: 0.5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 344C, 351C, 358C, 365C, 372C, 379C, 386C, 393C, 400C, 407C, 414C 421C, 428C, 435C, 442C, 44 9C, 456C, 463C, 470C, 477C, 484C, 491C 498C, 505C, 512C, 519C, 526C, 533C, 54 0C, 54 7C, 554C, 561C, 568C 575C, 582C, 58 9C, 596C, 603C 505C, 512C, 519C, 526C, 533C, 54 0C, 54 7C, 554C, 561C, 568C, 575C 582C, 58 9C, 596C, 603C 526C 1, 8 , 15, 22 323 330, 337, 344, 348 1, 8 , 15, 22, 29, 36, 43, 50, 57, 64 71C, 78C, 85C, 92C , 99C, 106C, 113C,, 120C,, 127C, 134C, 141C 148C, 155C , 162C, 16 9C, 176C, 183C, 190C, 197C, 204C, 211C, 218C 225C, 232C , 239C, 246C, 253C, 260C, 267C, 274C, 281C, 288C, 295C 302C, 309C , 316C, 323C, 330C, 337C, 344C, 348C 348C 901 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 357 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F2 DOSE: 0.5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92943 M 97 APPEARANCE HEAD TILT LEFT THIN EXCRETION FEW FECES NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) INGUINAL REGION-RIGHT WEEK 92 2 14 NORMAL NO REMARKABLE OBSERVATIONS 1 4 NORMAL NO REMARKABLE OBSERVATIONS 631, 638, 645, 652, 659, 666, 673, 677 673, 677 645, 677 1 , 8 ,r 15, 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582, 589, 596, 603, 610, 617 , 624 638C, 645C, 652C, 659C, 666C, 673C, 677C 1 , 8 , 15, 22, 29, 36, 43, 50, 57, 64, 71, 78 1 , 8 , 15, 22 902 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 358 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F2 DOSE: 0.5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92946 T 105 APPEARANCE PROLAPSED VAGINA NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) AXILLARY REGION-RIGHT WEEK 105 VENTRAL-ABDOMINAL WEEK 78 M 94 APPEARANCE MALOCCLUSION THIN EXCRETION FEW FECES 393, 400, 407, 421 , 428 1 , 8 ,r 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 414, 435, 442 , 449, 456, 463, 470 , 477, 484, 491, 498 , 505 512, 519, 526, 533 , 547, 554, 561, 568 , 575, 582, 589, 596 , 603 610, 617, 624, 631 , 638, 645, 652, 659 , 666, 673, 680, 687 , 694 701, 708, 715, 722 729, 732 540 99, 106, 113, 1 2 0 , 127, 134, 141, 148, 155, 162, 169, 176, 183 190, 197, 204, 2 1 1 , 218, 225, 232, 239, 246, 253, 260, 267, 274 281, 288, 295, 302, 309, 316, 323, 330, 337, 344, 351,. 358, 365 372, 379, 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456 463, 470, 477, 484, 491, 498, 505,. 512, 519, 526, 533,. 540, 547 554, 561, 568, 575, 582, 589, 596, 603, 610, 617, 624, 631, 638 645, 652, 656 656 903 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 359 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F2 (CONTINUED FROM PREVIOUS PAGE) C92947 M 94 NORMAL NO REMARKABLE OBSERVATIONS C92948 M 99 APPEARANCE THIN EXCRETION FEW FECES RESPIRATION IRREGULAR SKIN & PELAGE BLUE SKIN PAWS-ALL TAIL ROUGH HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) VENTRAL-CERVICAL WEEK 96 DOSE: 0.5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 1, 8 , 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92 673, 691 669, 673, 680, 691 669 669, 673 669, 673 687, 691 1, 8 , 15 113, 120 204, 211 295, 302 386, 393 477, 484 568, 575 659 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 582, 589, 596, 603, 610, 617 , 624, 631, 638, 645 , 652 666, 669C, 673C, 680C, 687C, 691C 904 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 360 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F2 DOSE: 0.5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92949 M 33 SKIN & PELAGE SORE/SCAB LATERAL-CERVICAL-RIGHT VENTRAL-CERVICAL-LEFT NORMAL NO REMARKABLE OBSERVATIONS C92950 1 C92951 M MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 3 WEEK 21 4 NORMAL NO REMARKABLE OBSERVATIONS 103 APPEARANCE MALOCCLUSION MISSING TEETH DISCHARGE GENITAL RED IN COLOR 8 229 1, 15 , 2 2 , 29, 36,, 43, 50, 57,, 64, 71, 78, 85, 92, 99, 106, 113 1 2 0 , 127, 134 19 0C, 197C , 204C, 211C, 218C, 225C, 229C 141C, 148C , 155C, 162C, 16 9C, 176C, 183C 1, 8 , 15, 22 148, 239, 365, 456, 547, 638, 155, 281, 372, 463, 554, 645, 162, 288, 379, 470, 561, 652, 169, 295, 386, 477, 568, 659, 176, 183, 302, 309, 393, 400, 484, 491, 575, 582, 666, 673, 190, 316, 407, 498, 589, 680, 197, 323, 414, 505, 596, 687, 204, 330, 421, 512, 603, 694, 2 1 1 , 218, 337, 344, 428, 435, 519, 526, 610, 617, 701, 708, 225, 351, 442, 533, 624, 715 232 358 449 540 631 183, 190, 197, 204, 2 1 1 , 218, 225, 232, 239, 246, 253, 260, 267 274, 281, 288, 295, 302, 309, 316, 323, 330, 337, 344, 351, 358 365, 372, 379, 386 491, 519 905 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 361 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F2 (CONTINUED FROM PREVIOUS PAGE) C92951 M 103 SKIN & PELAGE SORE/SCAB AXILLARY REGION-RIGHT NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) AXILLARY REGION-RIGHT WEEK 64 INGUINAL REGION-LEFT WEEK 64 VENTRAL-ABDOMINAL-RIGHT WEEK 80 C92952 M 66 BEHAVIOR HYPOACTIVE <UNSPECIFIED> MODERATE EXCRETION FEW FECES DOSE: 0.5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 498, 505, 512, 519, 526 , 533, 540, 547, 554, 561, 568 , 575 , 582 589, 596, 603, 610, 617 , 624, 631, 638, 645, 652, 659 , 666 , 673 680, 687, 694, 701, 708 , 715 1 , 8 , 15, 22, 29, 36, 43, 50, 57, 64, 71 , 78, 85, 92, 99, 106 113, 120, 127, 134, 141 442C, 449C, 456C, 463C, 470C, 477C , 484C , 491C, 498C, 505C , 512C 519C, 526C, 533C, 540C, 54 7C, 554C , 561C , 568C, 575C, 582C , 589C 596C, 603C, 610C, 617C, 624C, 631C , 638C , 645C, 652C, 659C , 666C 673C, 680C, 687C, 694C, 701C, 708C , 715C 442C, 449C, 456C, 463C, 470C, 477C , 484C , 491C, 498C, 505C , 512C 519C 554, 561C, 568C, 575C, 582C, 58 9C, 596C, 603C, 610C, 617C, 624C 631C, 638C, 645C, 652C, 659C, 666C , 673C , 680C, 687C, 694C , 701C 708C, 715C 456 456 456 906 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 362 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F2 (CONTINUED FROM PREVIOUS PAGE) C92952 M 66 EYES RED DISCHARGE EYES NORMAL NO REMARKABLE OBSERVATIONS C92953 T MASS(ES) AXILLARY REGION-LEFT WEEK 50 WEEK 57 WEEK 63 105 NORMAL NO REMARKABLE OBSERVATIONS DOSE: 0.5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 456 1 , 8 ,r 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 351 , 358, 365, 372, 379 , 386 421, 428 344 393, 400, 407, 414 435C,. 442C, 449C, 456C 1, 8 15, 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582, 589, 596, 603, 610, 617 , 624, 631, 638, 645 , 652 659, 666, 673, 680, 687, 694, 701, 708 , 715, 722, 729, 733 907 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 363 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F2 DOSE: 0.5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92954 M 101 EXCRETION NO FECES SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE HEAD-CRANIAL YELLOW HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) INGUINAL REGION-LEFT WEEK 76 VENTRAL-CERVICAL-RIGHT WEEK 77 C92955 M 92 APPEARANCE THIN BEHAVIOR ATAXIC <UNSPECIFIED> 701, 704 505, 512, 519,. 526, 533, 540, 547, 701 , 704 358, 365, 372, 379, 386, 393, 400, 407 , 414, 421 704 1 , 8 ,r 15, 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 428, 435, 442 , 449 456, 463, 470, 477, 484, 491, 498 526C, 533C, 54 0C, 54 7C, 554C, 561C, 568C, 575C 533C, 54 0C, 54 7C, 554C, 561C, 568C, 575C, 582C, 58 9C, 596C, 603C 610C, 617C, 624C, 631C, 638C, 645C, 652C, 659C, 666C, 673C, 680C 687C, 694C, 701C, 704C 633, 638 638 908 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 364 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F2 (CONTINUED FROM PREVIOUS PAGE) C92955 M 92 EXCRETION FEW FECES SKIN & PELAGE ROUGH HAIR COAT SORE/SCAB DORSAL-CERVICAL SHOULDER-RIGHT YELLOW HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS ^SS(ES) INGUINAL REGION-LEFT WEEK 75 INGUINAL REGION-RIGHT WEEK 76 WEEK 83 VENTRAL-ABDOMINAL WEEK 62 VENTRAL-ABDOMINAL-RIGHT WEEK 84 DOSE: 0.5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 633 512, 519, 526, 533 , 540, 547, 554, 561 , 568, 575, 582, 589, 596 603 526, 533, 540, 547 , 554, 561, 568, 575 , 582, 589, 596, 603, 610 617, 624, 631, 633 , 638 463, 470, 477, 484 , 491, 498 633, 638 1 , 8 ,r 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190, 197 204, 2 1 1 , 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281, 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372, 379 386, 393, 400, 407 , 414, 421, 435, 442 , 449, 456, 505 519C, 526C 533C, 54 0C, 54 7C, 554C, 561C, 568C, 575C, 582C, 589C 596C, 603C 610C, 617C, 624C, 631C, 633C, 638C 526 575C, 582C 58 9C, 596C, 603C, 610C, 617C, 624C, 631C, 633C, 638C 428C 582C, 589C 596C, 603C, 610C, 617C, 624C, 631C, 633C, 638C 909 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 365 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F2 DOSE: 0.5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92956 M 102 SKIN & PELAGE BLUE SKIN VENTRAL-ABDOMINAL-LEFT SORE/SCAB MOUTH VENTRAL-ABDOMINAL-LEFT NORMAL NO REMARKABLE OBSERVATIONS C92957 T MASS(ES) HEAD-MAXILLARY-LEFT WEEK 90 INGUINAL REGION-LEFT WEEK 82 NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 63 673, 680, 687, 694, 701, 708- 709 638, 645 701, 708-709 1, 8,. 15, 22, 29, 36, 43 , 50, 57, 64, 71, 78, 85, 92, 99, 106 120113, , 127, 134, 141, 148, 155, 162, 169, 176, 183,. 190, 197 211204, , 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365,. 372, 379 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456,. 463, 470 477, 484, 491, 498, 505, 512, 519, 526, 533, 540, 547,. 554, 561 624C, 631 568C, 575C , 582C, 589C, 596C, 603C , 610C, 617C, 624C, 631C, 638C 645C, 652C , 659C, 666C, 673C, 680C , 687C, 694C, 701C, 708-7 09C 1, 8,. 15, 22, 29, 36, 43 , 50, 57, 64, 71, 78, 85, 92, 99, 106 120113, , 127, 134, 141, 148, 155, 162, 169, 176, 183,. 190, 197 211204, , 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309 435C, 442C , 449C, 456C, 463C, 470C , 477C, 484C, 491C, 498C, 505C 512C, 519C , 526C, 533C, 54 0C, 547C , 554C, 561C, 568C, 575C, 582C 910 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 366 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F2 (CONTINUED FROM PREVIOUS PAGE) C92957 T 105 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 63 WART LIKE LESION(S) NOSE WEEK 46 C92958 M 89 APPEARANCE HEAD TILT RIGHT HUNCHED BEHAVIOR HYPOACTIVE <UNSPECIFIED> RECUMBENT LATERAL EXCRETION FEW FECES SKIN & PELAGE ROUGH HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS DOSE: 0.5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 58 9C, 596C, 603C, 610C, 617C, 624C, 631C, 638C, 645C, 652C, 659C 666C, 673C, 680C, 687C, 694C, 701C, 708C, 715C, 722C, 729C, 733C 316, 323, 330, 337, 344, 351, 358, 365, 372, 379, 386, 393, 400 407, 414, 421, 428, 435,, 442, 449, 456, 463, 470, 477,, 484, 491 498 610, 617, 619 603, 610, 617, 619 619 619 575, 582, 619 603, 610, 617, 619 1 , 8 ,r 15, 2 2 , 29, 36, 43,, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127,. 134, 141, 148, 155,. 162, 169, 176, 183,. 190, 197 204, 2 1 1 , 218,. 225, 232, 239, 246,. 253, 260, 267, 274, 281, 288 911 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 367 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F2 (CONTINUED FROM PREVIOUS PAGE) C92958 M 89 NORMAL NO REMARKABLE OBSERVATIONS C92959 M 98 APPEARANCE MALOCCLUSION MISSING TEETH EXCRETION FEW FECES NO FECES EYES RED DISCHARGE EYE-RIGHT SKIN & PELAGE BROWN HAIR COAT VENTRAL-ABDOMINAL SORE/SCAB MOUTH NORMAL NO REMARKABLE OBSERVATIONS DOSE: 0.5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470 477, 484, 491, 498, 505, 512, 519, 526, 533, 540, 547, 554, 561 568, 589, 596 407, 413- 414, 421, 428, 435, 442, 449, 456, 463, 470, 477, 484 491, 498, 505,. 512, 519,. 526,. 533,. 540, 547,. 554, 561,. 568, 575 582, 589, 596, 603,. 610, 617,. 624, 631,. 638, 645,. 652, 659, 666 673, 680 470, 477, 484, 491, 498, 505,. 512, 519,. 526,. 533,. 540, 547, 554 O (J1 CO CO O 561, 568, 575,. 582, . 596, 603,. 610, 617,. 624, 631,. 638, 645 652, 659, 666,. 673, 673 680 680 680 413-414, 421 1, 8 , 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92 99, 106 912 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 368 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F2 (CONTINUED FROM PREVIOUS PAGE) C92959 M 98 NORMAL NO REMARKABLE OBSERVATIONS T 105 NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) INGUINAL REGION-LEFT WEEK 72 C92961 M 99 APPEARANCE HEAD TILT LEFT LIMITED USE LIMB-HIND-LEFT THIN BEHAVIOR HYPOACTIVE <UNSPECIFIED> DOSE: 0.5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 113, 204, 295, 386, 12 0 , 127, 2 1 1 , 218, 302, 309, 393, 400 134, 225, 316, 141, 232, 323, 148, 239, 330, 155, 246, 337, 162, 253, 344, 169, 260, 351, 176, 267, 358, 183, 274, 365, 190 , 197 281 , 288 372 , 379 1 , 8 ,. 15, 2 2 , 29, 36, 43 , 50, 57, 64, 71, 78,, 85, 92, 99, 106 113, 1 2 0 , 127, 134, 141, 148, 155, 162, 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253, 260, 267, 274, 281 , 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463 , 470 477, 484, 491 498C, 575C, 652C, n72o c9mC-', 505C , 512C, 582C , 58 9C, 659C , 66 6C, n733Cn 519C, 596C, 673C, 526C, 603C, 680C, 533C , 540C, 610C , 617C, 687C , 694C, 547C, 624C, 701C, 554C, 631C, 708C, 561C , 568C 638C , 645C 715C , 722C 687, 691 687, 691 687, 691 687, 691 913 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 369 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F2 (CONTINUED FROM PREVIOUS PAGE) C92961 M 99 EXCRETION FEW FECES SKIN & PELAGE BLUE SKIN VENTRAL-ABDOMINAL-LEFT SORE/SCAB PAW-HIND-RIGHT NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) AXILLARY REGION-LEFT WEEK 45 AXILLARY REGION-RIGHT WEEK 36 WEEK 58 INGUINAL REGION-LEFT WEEK 76 DOSE: 0.5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 691 687, 691 526, 533, 540 1 , 8 ,r 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225 , 232, 239, 260, 267 , 274, 281, 288, 295 , 302 330, 337, 344, 351 , 358, 365, 372, 379 , 386, 393, 428, 435 , 442 449, 456, 463, 470 , 477, 484, 491, 498 , 505, 512, 519 309, 316, 323 246, 253 400, 407, 414, 421 526C, 533C , 540C, 547C, 554C, 561C, 568C, 575C, 582C, 58 9C, 596C 603C, 610C , 617C, 624C, 631C, 638C, 645C, 652C, 659C, 666C, 673C 680C, 687C , 691C 914 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 370 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F2 DOSE: 0.5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92962 2 M 14 NORMAL NO REMARKABLE OBSERVATIONS 94 APPEARANCE MALOCCLUSION EYES PROTRUDING EYES SKIN & PELAGE BLACK SKIN VENTRAL-ABDOMINAL-RIGHT PALE ENTIRE BODY SORE/SCAB VENTRAL-ABDOMINAL-RIGHT NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) AXILLARY REGION-LEFT WEEK 72 1 , 8 ,r 15, 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92-93 365, 372, 379,. 386, 393, 400, 407, 414 , 421, 428, 435, 442, 449 456, 463, 470, 477, 484, 491, 498, 505 , 512, 519, 526, 533, 540 547, 554, 561,. 568, 575, 582, 589, 596 , 603, 610, 617, 624, 631 638, 645, 652, 656 519 652, 656 656 656 1 , 8 ,r 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127, 134 , 141, 148, 155, 162 , 169, 176, 183,. 190, 197 204, 2 1 1 , 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281, 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358 498C, 505C, 512C, 519C, 526C, 533C, 54 0C, 54 7C, 554C, 561C, 568C 575C, 582C, 58 9C, 596C, 603C, 610C, 617C, 624C, 631C, 638C, 645C 652C, 656C 915 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 371 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F2 (CONTINUED FROM PREVIOUS PAGE) C92963 M 94 MASS(ES) INGUINAL REGION-RIGHT WEEK 75 VENTRAL-ABDOMINAL-LEFT WEEK 83 VENTRAL-ABDOMINAL-RIGHT WEEK 68 T 105 APPEARANCE MALOCCLUSION EXCRETION FEW FECES NONFORMED FECES NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) VENTRAL-CERVICAL WEEK 83 DOSE: 0.5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 519C, 526C , 533C, 540C, 547C, 554C , 561C, 568C, 575C, 582C , 589C 596C, 603C , 610C, 617C, 624C, 631C , 638C, 645C, 652C 575, 582C, 589C, 596C, 603C, 610C, 617C, 624C , 631C, 638C, 645C 652C, 656C 470C, 477C , 484C, 491C, 498C, 505C , 512C, 519C, 526C, 533C , 540C 54 7C, 554C , 561C, 568C, 575C, 582C , 589C, 596C, 603C, 610C , 617C 624C, 631C , 638C, 645C, 652C, 656C 512, 519, 526, 533, 540, 547, 554, 561, 568, 575, 582 , 589 , 596 603, 610, 617, 624, 631, 638, 645, 652, 659, 666, 673 , 680 , 687 694, 701, 708, 715, 722, 729, 731 540 400 1 , 8 ,. 15, 22, 29, 36, 43, 50, 113, 1 2 0 , 127, 134, 141, 148, 204, 2 1 1 , 218, 225, 232, 239, 295, 302, 309, 316, 323, 330, 386, 393, 407, 414, 421, 428, 484, 491, 498, 505 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 435, 442, 449, 456, 92, 99, 106 183 , 190 , 197 274 , 281 , 288 365 , 372 , 379 463 , 470 , 477 575, 582C, 589C, 596C, 603C, 610C, 617C, 624C , 631C, 638C, 645C 916 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 372 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F2 (CONTINUED FROM PREVIOUS PAGE) C92964 T 105 MASS(ES) VENTRAL-CERVICAL WEEK 83 C92965 D 82 APPEARANCE HUNCHED THIN EXCRETION NO FECES EYES RED DISCHARGE EYES NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) VENTRAL-ABDOMINAL WEEK 60 DOSE: 0.5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 652C,. 659C, 666C, 673C, 680C, 687C, 694C, 701C, 708C, 715C, 722C 72 9C, 731C 570, 573 561, 568, 570, 573 570, 573 547, 554, 561,. 568, 570, 573 1 , 8 ,. 15, 2 2 , 29, 36, 43 , 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127,. 134, 141, 148, 155, 162, 169, 176, 183, 190 , 197 204, 2 1 1 , 218,. 225, 232, 239, 246, 253, 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344, 351, 358, 365, 372 , 379 386, 393, 400, 407 414C, 421C , 428C, 435C, 442C, 449C , 456C, 463C, 470C, 477C , 484C 491C, 498C , 505C, 512C, 519C, 526C , 533C, 540C, 547C, 554C , 561C 56 8C, 57 0C, 573C 917 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 373 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F2 DOSE: 0.5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92966 M 72 APPEARANCE HEAD TILT RIGHT THIN BEHAVIOR ATAXIC <UNSPECIFIED> EYES RED DISCHARGE EYE-RIGHT SKIN & PELAGE ROUGH HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS C92967 2 14 NORMAL NO REMARKABLE OBSERVATIONS 502 498, 502 502 316 498, 502 1 , 8 , 15 113, 120 204, 211 295, 302 393, 400 484, 491 22, 29, 36, 43,, 50, 57, 64, 71, 78, 85, 92, 99, 106 127, 134, 141, 148, 155, 162 , 169, 176, 183 , 190 , 197 218, 225, 232, 239, 246, 253 , 260, 267, 274 , 281 , 288 309, 323, 330, 337, 344, 351 , 358, 365, 372 , 379 , 386 407, 414, 421, 428, 435, 442 , 449, 456, 463 , 470 , 477 1 , 8 , 15 22, 29, 36, 43,, 50, 57, 64, 71, 78, 85, 92- 93 918 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 374 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F2 DOSE: 0.5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92968 D 69 EXCRETION FEW FECES EYES DILATED PUPIL EYES RED DISCHARGE EYES NORMAL NO REMARKABLE OBSERVATIONS C92969 1 C92970 T 4 NORMAL NO REMARKABLE OBSERVATIONS 105 APPEARANCE MALOCCLUSION THIN DISCHARGE NASAL RED IN COLOR EXCRETION FEW FECES 470, 477 470, 477 470, 477 1 , 8 ,r 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407 , 414, 421, 428, 435 , 442, 449, 456, 463 1, 8 , 15, 22 78 715, 722, 729, 731 722 715, 722, 729, 731 919 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 375 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F2 (CONTINUED FROM PREVIOUS PAGE) C92970 T 105 EYES PROTRUDING EYES SKIN & PELAGE SORE/SCAB PAW-HIND-RIGHT NORMAL NO REMARKABLE OBSERVATIONS C92 971 M 90 APPEARANCE MALOCCLUSION MISSING TEETH THIN SKIN & PELAGE SORE/SCAB VENTRAL-ABDOMINAL-LEFT DOSE: 0.5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 519 652, 659, 666 , 673 , 680, 687, 694, 701 , 708, 715, 722, 729, 731 1 , 8 ,r 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 85,r 92, 99, 106, 113 1 2 0 , 127, 134, 141 , 148, 155, 162, 169 , 176, 183, 190, 197, 204 2 1 1 , 218, 225, 232 , 239, 246, 253, 260 , 267, 274, 281, 288, 295 302, 309, 316, 323 , 330, 337, 344, 351 , 358, 365, 372, 379, 386 393, 400, 407, 414 , 421, 428, 435, 442 , 449, 456, 463, 470, 477 484, 491, 498, 505 , 512, 526, 533, 540 , 547, 554, 561, 568, 575 582, 589, 596, 603 , 610, 617, 624, 631 , 638, 645 155, 246, 337, 428, 519, 610, 162, 253, 344, 435, 526, 617, 169, 260, 351, 442, 533, 624 176, 267, 358, 449, 540, 183, 274, 365, 456, 547, 190, 281, 372, 463, 554, 197, 288, 379, 470, 561, 204, 295, 386, 477, 568, 2 1 1 , 218, 302, 309, 393, 400, 484, 491, 575, 582, 225, 316, 407, 498, 589, 232, 323, 414, 505, 596, 239 330 421 512 603 554, 561, 568, 575, 582, 589, 596, 603 624 617, 624 920 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 376 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F2 (CONTINUED FROM PREVIOUS PAGE) C92971 M 90 NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) INGUINAL REGION-LEFT WEEK 67 C92972 M 96 APPEARANCE THIN BEHAVIOR HYPOACTIVE <UNSPECIFIED> EXCRETION FEW FECES EYES RED DISCHARGE EYE-RIGHT EYES NORMAL NO REMARKABLE OBSERVATIONS DOSE: 0.5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 1 , 8 , 15, 22, 29, 36, 43, 50, 57, 64 , 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127, 134, 141, 148 463C, 470C , 477C, 484C, 491C, 498C, 505C, 512C , 519C, 526C , 533C 54 0C, 54 7C , 554C, 561C, 568C, 575C, 582C, 589C , 596C, 603C , 610C 617C, 624C 666, 670 670 670 435, 442, 449, 456 , 463, 470, 477, 484 , 491, 498, 505, 512 , 519 526, 533, 540, 547 , 554, 561, 568, 575 , 582, 589, 596, 603 , 610 617, 624, 631, 638 , 645, 652, 659, 666 670 1 , 8 ,r 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 365, 372, 379 , 386, 393, 400, 407 , 414 921 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 377 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F2 (CONTINUED FROM PREVIOUS PAGE) C92972 M 96 NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) SHOULDER-RIGHT WEEK 72 C92973 T VENTRAL-ABDOMINAL-RIGHT WEEK 48 105 NORMAL NO REMARKABLE OBSERVATIONS C92974 T 105 NORMAL NO REMARKABLE OBSERVATIONS DOSE: 0.5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 421, 428 498C, 505C , 512C, 519C, 526C, 533C, 54 0C, 54 7C, 554C, 561C, 568C 575C, 582C , 589C, 596C, 603C, 610C, 617C, 624C, 631C, 638C, 645C 652C, 659C , 666C, 670C 330, 337, 344, 351 , 358 1 , 8 ,r 15, 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582, 589, 596, 603, 610, 617 , 624, 631, 638, 645 , 652 659, 666, 673, 680, 687, 694, 701, 708 , 715, 722, 729, 731 1 , 8 ,r 15, 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582, 589, 596, 603, 610, 617 , 624, 631, 638, 645 , 652 659, 666, 673, 680, 687, 694, 701, 708 , 715 922 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 378 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F2 (CONTINUED FROM PREVIOUS PAGE) C92974 T 105 MASS(ES) VENTRAL-CERVICAL-RIGHT WEEK 104 C92975 T 105 NORMAL NO REMARKABLE OBSERVATIONS C92976 M MASS(ES) VENTRAL-ABDOMINAL WEEK 89 VENTRAL-ABDOMINAL-RIGHT WEEK 47 102 APPEARANCE HEAD TILT LEFT EXCRETION FEW FECES EYES CLEAR DISCHARGE EYE-RIGHT RED DISCHARGE EYE-LEFT DOSE: 0.5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 722, 729, 732 221, 8 15, , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 120113, , 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 211204, , 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 330, 337, 344, 351 , 358, 365, 372, 379 , 386 393, 400, 407, 414 , 421, 428, 435, 442 , 449, 456, 463, 470 , 477 484, 491, 498, 505 , 512, 519, 526, 533 , 540, 547, 554, 561 , 568 575, 582, 589, 596 , 603, 610 617C 624C, 631C, 638C, 645C, 652C, 659C, 666C, 673C, 680C, 687C 694C 701C, 708C, 715C, 722C, 729C, 733C 323C 712 712 680, 687 712 923 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 379 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F2 (CONTINUED FROM PREVIOUS PAGE) C92976 M 102 EYES RED DISCHARGE EYE-RIGHT NORMAL NO REMARKABLE OBSERVATIONS CO CO MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 83 WEEK D 98 SKIN & PELAGE SORE/SCAB PAW-HIND-RIGHT NORMAL NO REMARKABLE OBSERVATIONS DOSE: 0.5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 694, 701, 708 1 , 8 ,r 15, 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568 575C, 582C, 58 9C, 596C, 603C, 610C, 617C, 624C, 631C, 638C, 645C 652C, 659C, 666C, 673C, 680C, 687C, 694C, 701C, 708C, 712C 610C, 617C, 624C, 631C, 638C, 645C, 652C, 659C, 666C, 673C, 680C 687C, 694C, 701C 673, 680, 686 1 , 8 ,r 15, 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582, 589, 596, 603, 610, 617 , 624, 631, 638, 645 , 652 924 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 380 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F2 (CONTINUED FROM PREVIOUS PAGE) C92977 D 98 NORMAL NO REMARKABLE OBSERVATIONS C92978 2 14 NORMAL NO REMARKABLE OBSERVATIONS C92979 M 89 BEHAVIOR COLD TO TOUCH ENTIRE BODY DISCHARGE GENITAL RED IN COLOR EXCRETION NO FECES NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) AXILLARY REGION-RIGHT WEEK 80 DOSE: 0.5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 659, 666 1 , 8 , 15, 22, 29, 36, 43, 50, 57, 64, 71, 78 622 622 617, 622 1 , 8 ,r 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0, 127 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400 407 , 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491 498 , 505, 512, 519, 526 , 533, 540, 547 554, 561C, 568C, 575C, 582C, 589C, 596C, 603C, 610C, 617C, 622C 925 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 381 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F2 DOSE: 0.5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92980 M 63 APPEARANCE MALOCCLUSION SKIN & PELAGE SORE/SCAB AXILLARY REGION-LEFT NORMAL NO REMARKABLE OBSERVATIONS C92981 M MASS(ES) AXILLARY REGION-LEFT WEEK 38 LIMB-HIND-RIGHT WEEK 55 72 APPEARANCE HEAD TILT LEFT BEHAVIOR HYPOACTIVE <UNSPECIFIED> EXCRETION NO FECES 204, 2 1 1 , 218, 260, 281, 288, 316 , 337 , 344, 351, 358, 365, 400 407, 414, 421,. 428, 435 316, 323, 330, 337, 344, 351, 358 , 365 , 372, 379, 386, 393, 400 407, 414, 421,. 428, 435 1 , 8 ,r 15, 2 2 , 29, 36 , 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0, 127,. 134, 141, 148, 155 , 162 , 169, 176, 183, 190, 197 225, 232, 239,. 246, 253 260, 267, 274, 281C, 288C, 295C, 302C, 309C, 316C, 323C, 330C 337C, 344C, 351C, 358C, 365C, 372C, 379C, 386C, 393C, 400C, 407C 414C, 421C, 428C, 435C 379, 386, 393C, 400C, 407C, 414C, 421C, 428C, 435C 502 502 502 926 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 382 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F2 (CONTINUED FROM PREVIOUS PAGE) C92981 M 72 SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE ROUGH HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS C92982 1 C92983 T 4 NORMAL NO REMARKABLE OBSERVATIONS 105 EXCRETION FEW FECES SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE NORMAL NO REMARKABLE OBSERVATIONS DOSE: 0.5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 477, 484, 502 498, 502 1 , 8 , 15 113, 120 204, 211 295, 302 386, 393 491 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 400, 407 , 414, 421, 428, 435 , 442, 449, 456, 463 , 470 1, 8 , 15, 22 729, 731 722 1 , 8 ,r 15, 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582, 589, 596, 603, 610, 617 , 624, 631, 638, 645 927 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 383 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F2 (CONTINUED FROM PREVIOUS PAGE) C92983 T 105 MASS(ES) INGUINAL REGION-LEFT WEEK 94 VENTRAL-CERVICAL WEEK 96 C92984 T 105 SKIN & PELAGE SORE/SCAB PAW-HIND-LEFT NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) AXILLARY REGION-LEFT WEEK 75 WEEK 93 INGUINAL REGION-RIGHT WEEK 75 VENTRAL-ABDOMINAL WEEK 60 WEEK 104 VENTRAL-ABDOMINAL-LEFT WEEK 75 DOSE: 0.5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 652C, 659C, 666C, 673C, 680C, 687C , 694C, 701C, 708C, 715C , 722C 729C, 731C 666, 673, 680, 687, 694, 701, 708, 715, 722, 729, 731 631 1 , 8 ,. 15, 22, 29, 36, 43 , 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183 , 190 , 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274 , 281 , 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365 , 372 , 379 386, 393, 400, 407, 463, 470, 477, 484, 491, 498, 505 , 512 519, 526 64 5C, 652C, 659C, 666C, 673C, 680C , 687C, 694C, 701C, 708C , 715C 722C,. 729C, 732C 519, 526, 533, 540, 547, 554, 561, 568C, 575C, 582C, 58 9C, 596C 603C, 610C, 617C, 624C, 631C, 638C , 645C, 652C, 659C, 666C , 673C 680C,. 687C, 694C, 701C, 708C, 715C , 722C, 729C, 732C 414C, 421C, 428C, 435C, 442C, 449C , 456C 722C,. 729C, 732C 519, 526 928 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 384 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F2 (CONTINUED FROM PREVIOUS PAGE) C92984 T 105 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 75 C92985 M 98 APPEARANCE MALOCCLUSION MISSING TEETH NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) AXILLARY REGION-RIGHT WEEK 76 INGUINAL REGION-RIGHT WEEK 96 VENTRAL-ABDOMINAL-LEFT WEEK 82 DOSE: 0.5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 519, 526 , 533C , 540C, 547C, 554C, 561C, 568C, 575C, 582C, 589C 596C, 60 3C, 610C, 617C, 624C , 631C , 638C , 645C, 652C, 659C , 666C 673C, 68 0C, 687C, 694C, 701C , 708C , 715C , 722C, 729C, 732C 155, 246, 365, 456, 547, 638, 162 , 169, 253 , 260, 372 , 379, 463 , 470, 554 , 561, 645 , 652, 176, 267, 386, 477, 568, 659, 183, 190 , 197, 274, 281 , 288, 393, 400 , 407, 484, 491 , 498, 575, 582 , 589, 666, 673 , 680 204, 295, 414, 505, 596, 2 1 1 , 218 , 225, 302, 309 , 316, 421, 428 , 435, 512, 519 , 526, 603, 610 , 617, 232 , 239 351 , 358 442 , 449 533 , 540 624 , 631 204, 211 , 526, 533, 540, 547 , 554, 561, 568, 575 , 582, 589 , 596 603, 610 , 617, 624, 631, 638 , 645, 652, 659, 666 , 673, 680 1 , 8 , 15 , 2 2 , 29, 36, 43 , 50 , 57, 64, 71 , 78, 85 , 92, 99, 106 113, 120 , 127, 134, 141, 148 , 323, 330, 337, 344 526C, 533C, 54 0C, 54 7C, 554C, 561C, 568C, 575C, 582C, 58 9C, 596C 603C, 610C, 617C, 624C, 631C, 638C, 645C, 652C, 659C, 666C, 673C 680C 666C, 673C, 680C 568C, 575C, 582C, 58 9C, 596C, 603C, 610C, 617C, 624C, 631C, 638C 645C, 652C, 659C, 666C, 673C, 680C 929 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 385 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F2 (CONTINUED FROM PREVIOUS PAGE) C92985 M 98 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 95 VENTRAL-THORACIC-LEFT WEEK 95 C92986 T 105 SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) INGUINAL REGION-LEFT WEEK 85 INGUINAL REGION-RIGHT WEEK 76 WEEK 100 DOSE: 0.5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 659C, 666C, 673C, 680C 659C, 666C, 673C, 680C 519, 526, 533, 540, 547 1 , 8 ,r 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407 , 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498 , 505, 512, 561, 568 , 575, 582 58 9C, 596C, 603C, 610C, 617C, 624C, 631C, 638C, 645C, 652C, 659C 666C, 673C, 680C, 687C, 694C, 701C, 708C, 715C, 722C, 729C, 733C 526C, 533C, 54 0C, 54 7C, 554C 694C, 701C, 708C, 715C 930 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 386 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F2 DOSE: 0.5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92987 2 C92988 T 14 NORMAL NO REMARKABLE OBSERVATIONS 105 NORMAL NO REMARKABLE OBSERVATIONS M 84 APPEARANCE MALOCCLUSION SWOLLEN MOUTH DISCHARGE GENITAL RED IN COLOR NASAL RED IN COLOR EXCRETION NO FECES EYES RED DISCHARGE EYE-RIGHT 1 , 8 ,r 15, 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92- 93 1 , 8 ,r 15, 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0, 127,. 134, 141, 148, 155, 162 , 169, 176, 183 , 190 , 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253 , 260, 267, 274 , 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365 , 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456 , 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547 , 554 , 561 568, 575, 582, 589, 596, 603, 610, 617 , 624, 631, 638 , 645 , 652 659, 666, 673, 680, 687, 694, 701, 708 , 715, 722, 729 , 731 64, 358, 365, 372, 379, 386, 393, 400, 407, 414, 421, 428, 435 442, 449,. 456,. 463,. 470, 477,. 484, 491, 498, 505,, 512,, 540, 547 554, 561,. 568,. 575, 582 358, 365 547 358 582 519, 526, 533 931 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 387 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F2 (CONTINUED FROM PREVIOUS PAGE) C92989 M 84 SKIN & PELAGE BROWN HAIR COAT VENTRAL-ABDOMINAL NORMAL NO REMARKABLE OBSERVATIONS M 94 NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) AXILLARY REGION-LEFT WEEK 73 VENTRAL-ABDOMINAL WEEK 63 VENTRAL-CERVICAL-RIGHT WEEK 89 DOSE: 0.5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 582 1 , 8 ,r 15, 2 1 -22, 29, 36, 43, 50, 57, 71, 78, 85, 92, 99, 106 113, 1 2 0, 127 , 134, 141, 148, 155 , 162, 169, 176, 183 , 190, 197 204, 2 1 1 , 218 , 225, 232, 239, 246 , 253, 260, 267, 274 , 281, 288 295, 302, 309 , 316, 323, 330, 337 , 344, 351 1 , 8 ,r 15, 2 2 , 29, 36 , 43,r 50, 113, 1 2 0, 127 , 134, 141, 148, 204, 2 1 1 , 218 , 225, 232, 239, 295, 302, 309 , 316, 323, 330, 386, 393, 400 , 407, 414, 421, 57, 64, 71., 78, 85, 155 , 162, 169, 176, 246 , 253, 260, 267, 337 , 344, 351, 358, 428 , 463, 470, 477, 92, 99, 106 183 , 190, 197 274 , 281, 288 365 , 372, 379 484 , 491, 498 505C , 512C, 519C, 526C, 533C, 540C, 547C, 554C, 561C, 568C, 575C 582C , 589C, 596C, 603C, 610C, 617C, 624C, 631C, 638C, 645C 652- 653C 435C , 442C, 44 9C, 456C 617C , 624C, 631C, 638C, 645C, 652-653C 932 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 69 MASS(ES) 70 AXILLARY REGION-RIGHT WEEK 69 29 MASS(ES) 38 DORSAL WEEK 29 39 MASS(ES) 40 DORSAL WEEK 29 18 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 18 19 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 18 20 MASS(ES) 21 VENTRAL-ABDOMINAL-LEFT WEEK 18 22 MASS(ES) 28 VENTRAL-ABDOMINAL-LEFT WEEK 18 29 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 18 15 X 15MM 20 X 20MM 35 X 30MM MASS 30 X 25MM 25 X 20MM 30 X 15MM 50 X 15MM 35 X 10MM 933 Covance 6329-183 3M T-6295 PAGE: 388 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 30 MASS(ES) 31 VENTRAL-ABDOMINAL-LEFT WEEK 18 32 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 18 33 MASS(ES) 34 VENTRAL-ABDOMINAL-LEFT WEEK 18 35 MASS(ES) 38 VENTRAL-ABDOMINAL-LEFT WEEK 18 39 MASS(ES) 41 VENTRAL-ABDOMINAL-LEFT WEEK 18 25 MASS(ES) 29 VENTRAL-ABDOMINAL-RIGHT WEEK 25 30 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 25 31 MASS(ES) 32 VENTRAL-ABDOMINAL-RIGHT WEEK 25 35 X 15MM 35 X 20MM 45 X 45MM 35 X 40MM 45 X 35MM 25 X 20MM 30 X 25MM 30 X 30MM 934 Covance 6329-183 3M T-6295 PAGE: 389 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 33 MASS(ES) 37 VENTRAL-ABDOMINAL-RIGHT WEEK 25 38 MASS(ES) 40 VENTRAL-ABDOMINAL-RIGHT WEEK 25 41 MASS(ES) 41 VENTRAL-ABDOMINAL-RIGHT WEEK 25 25 MASS(ES) 28 VENTRAL-THORACIC-LEFT WEEK 25 64 MASS(ES) AXILLARY REGION-LEFT WEEK 64 65 MASS(ES) 67 AXILLARY REGION-LEFT WEEK 64 68 MASS(ES) 80 AXILLARY REGION-LEFT WEEK 64 81 MASS(ES) AXILLARY REGION-LEFT WEEK 64 35 X 30MM 40 X 35MM 50 X 50MM 10 X 10MM 20 X 10MM 20 X 20MM 40 X 40MM 45 X 45MM 935 Covance 6329-183 3M T-6295 PAGE: 390 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 82 MASS(ES) 88 AXILLARY REGION-LEFT WEEK 64 89 MASS(ES) 90 AXILLARY REGION-LEFT WEEK 64 91 MASS(ES) 92 AXILLARY REGION-LEFT WEEK 64 74 MASS(ES) 75 INGUINAL REGION-RIGHT WEEK 74 76 MASS(ES) INGUINAL REGION-RIGHT WEEK 74 77 MASS(ES) 80 INGUINAL REGION-RIGHT WEEK 74 81 MASS(ES) 85 INGUINAL REGION-RIGHT WEEK 74 86 MASS(ES) INGUINAL REGION-RIGHT WEEK 74 55 X 45MM 60 X 50MM 50 X 50MM 15 X 15MM 20 X 15MM 20 X 20MM 40 X 35MM 55 X 35MM 936 Covance 6329-183 3M T-6295 PAGE: 391 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 87 MASS(ES) 88 INGUINAL REGION-RIGHT WEEK 74 89 MASS(ES) 90 INGUINAL REGION-RIGHT WEEK 74 91 MASS(ES) 92 INGUINAL REGION-RIGHT WEEK 74 81 MASS(ES) 82 AXILLARY REGION-RIGHT WEEK 81 83 MASS(ES) 84 AXILLARY REGION-RIGHT WEEK 81 85 MASS(ES) 87 AXILLARY REGION-RIGHT WEEK 81 65 MASS(ES) 67 SHOULDER-RIGHT WEEK 65 63 MASS(ES) AXILLARY REGION-LEFT WEEK 63 65 X 50MM 75 X 75MM 70 X 70MM 65 X 60MM 30 X 30MM 50 X 50MM 60 X 60MM 10 X 15MM 937 Covance 6329-183 3M T-6295 PAGE: 392 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 64 MASS(ES) 66 AXILLARY REGION-LEFT WEEK 63 67 MASS(ES) AXILLARY REGION-LEFT WEEK 63 68 MASS(ES) 70 AXILLARY REGION-LEFT WEEK 63 71 MASS(ES) AXILLARY REGION-LEFT WEEK 63 72 MASS(ES) 73 AXILLARY REGION-LEFT WEEK 63 74 MASS(ES) AXILLARY REGION-LEFT WEEK 63 75 MASS(ES) AXILLARY REGION-LEFT WEEK 63 76 MASS(ES) 78 AXILLARY REGION-LEFT WEEK 63 20 X 20MM 25 X 25MM 30 X 40MM 30 X 20MM 40 X 30MM 30 X 50MM 40 X 40MM 50 X 35MM 938 Covance 6329-183 3M T-6295 PAGE: 393 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 79 MASS(ES) 80 AXILLARY REGION-LEFT WEEK 63 81 MASS(ES) 82 AXILLARY REGION-LEFT WEEK 63 83 MASS(ES) 85 AXILLARY REGION-LEFT WEEK 63 86 MASS(ES) AXILLARY REGION-LEFT WEEK 63 87 MASS(ES) 88 AXILLARY REGION-LEFT WEEK 63 89 MASS(ES) 91 AXILLARY REGION-LEFT WEEK 63 92 MASS(ES) AXILLARY REGION-LEFT WEEK 63 93 MASS(ES) 96 AXILLARY REGION-LEFT WEEK 63 40 X 45MM 40 X 60MM 40 X 50MM 35 X 50MM 50 X 50MM 60 X 50MM 60 X 35MM 60 X 40MM 939 Covance 6329-183 3M T-6295 PAGE: 394 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C92936 C92937 F2 673 97 MASS(ES) AXILLARY REGION-LEFT WEEK 63 680 98 MASS(ES) AXILLARY REGION-LEFT WEEK 63 687 99 MASS(ES) AXILLARY REGION-LEFT WEEK 63 694 100 MASS(ES) AXILLARY REGION-LEFT WEEK 63 701 101 MASS(ES) AXILLARY REGION-LEFT WEEK 63 708 102 MASS(ES) TO 732 105 AXILLARY REGION-LEFT WEEK 63 F2 428 62 TO 450 65 MASS(ES) VENTRAL-ABDOMINAL WEEK 61 295 43 TO 316 46 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 43 40 X 40MM 50 X 40 50 X 40MM 50 X 40MM 50 X 40MM 50 X 40MM 15 X 15MM 10 X 10MM 940 Covance 6329-183 3M T-6295 PAGE: 395 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 62 MASS(ES) 63 AXILLARY REGION-LEFT WEEK 58 64 MASS(ES) 69 AXILLARY REGION-LEFT WEEK 58 70 MASS(ES) AXILLARY REGION-LEFT WEEK 58 71 MASS(ES) 71 AXILLARY REGION-LEFT WEEK 58 63 MASS(ES) 65 VENTRAL-ABDOMINAL-RIGHT WEEK 63 66 MASS(ES) 67 VENTRAL-ABDOMINAL-RIGHT WEEK 63 68 MASS(ES) 69 VENTRAL-ABDOMINAL-RIGHT WEEK 63 70 MASS(ES) 71 VENTRAL-ABDOMINAL-RIGHT WEEK 63 15 X 15MM 20 X 20MM 20 X 35MM 30 X 30MM 10 X 10MM 15 X 15MM 30 X 25MM 15 X 15MM 941 Covance 6329-183 3M T-6295 PAGE: 396 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 47 MASS(ES) 54 VENTRAL-CERVICAL-RIGHT WEEK 47 55 MASS(ES) 57 VENTRAL-CERVICAL-RIGHT WEEK 47 58 MASS(ES) 59 VENTRAL-CERVICAL-RIGHT WEEK 47 60 MASS(ES) 61 VENTRAL-CERVICAL-RIGHT WEEK 47 62 MASS(ES) 67 VENTRAL-CERVICAL-RIGHT WEEK 47 68 MASS(ES) 69 VENTRAL-CERVICAL-RIGHT WEEK 47 70 MASS(ES) VENTRAL-CERVICAL-RIGHT WEEK 47 71 MASS(ES) 71 VENTRAL-CERVICAL-RIGHT WEEK 47 20 X 25MM 30 X 25MM 30 X 40MM 35 X 35MM 40 X 40MM 60 X 50MM 50 X 50MM 30 X 60MM 942 Covance 6329-183 3M T-6295 PAGE: 397 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 50 MASS(ES) AXILLARY REGION-LEFT WEEK 50 51 MASS(ES) AXILLARY REGION-LEFT WEEK 50 52 MASS(ES) 55 AXILLARY REGION-LEFT WEEK 50 56 MASS(ES) 57 AXILLARY REGION-LEFT WEEK 50 58 MASS(ES) 60 AXILLARY REGION-LEFT WEEK 50 61 MASS(ES) 64 AXILLARY REGION-LEFT WEEK 50 65 MASS(ES) 67 AXILLARY REGION-LEFT WEEK 50 68 MASS(ES) 73 AXILLARY REGION-LEFT WEEK 50 15 X 15MM 20 X 20MM 30 X 30MM 40 X 40MM 40 X 50MM 60 X 50MM 65 X 85MM 85 X 85MM 943 Covance 6329-183 3M T-6295 PAGE: 398 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT F2 512 74 TO 519 75 526 76 TO 568 82 575 83 582 84 TO 603 87 505 73 TO 512 74 519 75 526 76 TO 540 78 547 79 TO 561 81 MASS(ES) AXILLARY REGION-LEFT WEEK 50 MASS(ES) AXILLARY REGION-LEFT WEEK 50 MASS(ES) AXILLARY REGION-LEFT WEEK 50 MASS(ES) AXILLARY REGION-LEFT WEEK 50 MASS(ES) INGUINAL REGION-LEFT WEEK 73 MASS(ES) INGUINAL REGION-LEFT WEEK 73 MASS(ES) INGUINAL REGION-LEFT WEEK 73 MASS(ES) INGUINAL REGION-LEFT WEEK 73 90 X 90MM 100 X 90MM 110 X 90MM 100 X 90MM 20 X 20MM 30 X 30MM 40 X 30MM 40 X 40MM 944 Covance 6329-183 3M T-6295 PAGE: 399 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 82 MASS(ES) INGUINAL REGION-LEFT WEEK 73 83 MASS(ES) 85 INGUINAL REGION-LEFT WEEK 73 86 MASS(ES) INGUINAL REGION-LEFT WEEK 73 87 MASS(ES) 87 INGUINAL REGION-LEFT WEEK 73 76 MASS(ES) VENTRAL-THORACIC-RIGHT WEEK 76 11 MASS(ES) DORSAL WEEK 11 12 MASS(ES) 14 DORSAL WEEK 11 15 MASS(ES) 30 DORSAL WEEK 11 50 X 40MM 50 X 60MM 50 X 70MM 60 X 70MM 10 X 10MM 30 X 30MM 30 X 30MM 40 X 30MM 945 Covance 6329-183 3M T-6295 PAGE: 400 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 31 MASS(ES) 32 DORSAL WEEK 11 33 MASS(ES) 37 DORSAL WEEK 11 38 MASS(ES) DORSAL WEEK 11 39 MASS(ES) 40 DORSAL WEEK 11 41 MASS(ES) 42 DORSAL WEEK 11 43 MASS(ES) 45 DORSAL WEEK 11 46 MASS(ES) DORSAL WEEK 11 47 MASS(ES) 50 DORSAL WEEK 11 30 X 30MM 35 X 35MM 40 X 35MM 45 X 45MM 50 X 50MM 60 X 50MM 80 X 60MM 85 X 80MM 946 Covance 6329-183 3M T-6295 PAGE: 401 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 50 MASS(ES) 50 VENTRAL-ABDOMINAL-RIGHT WEEK 50 92 MASS(ES) 95 INGUINAL REGION-RIGHT WEEK 92 96 MASS(ES) 97 INGUINAL REGION-RIGHT WEEK 92 96 MASS(ES) 99 VENTRAL-CERVICAL WEEK 96 28 MASS(ES) 29 VENTRAL-CERVICAL-LEFT WEEK 3 30 MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 3 31 MASS(ES) 32 VENTRAL-CERVICAL-LEFT WEEK 3 33 MASS(ES) 33 VENTRAL-CERVICAL-LEFT WEEK 3 35 X 25MM 15 X 10MM 20 X 20MM 10 X 10MM 50 X 35MM 60 X 35MM 70 X 50MM 80 X 60MM 947 Covance 6329-183 3M T-6295 PAGE: 402 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 21 MASS(ES) 24 VENTRAL-CERVICAL-LEFT WEEK 21 25 MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 21 26 MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 21 27 MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 21 64 MASS(ES) 67 AXILLARY REGION-RIGHT WEEK 64 68 MASS(ES) 69 AXILLARY REGION-RIGHT WEEK 64 70 MASS(ES) AXILLARY REGION-RIGHT WEEK 64 71 MASS(ES) 74 AXILLARY REGION-RIGHT WEEK 64 15 X 15MM 25 X 25MM 35 X 25MM 40 X 25MM 10 X 10MM 15 X 15MM 25 X 25MM 20 X 15MM 948 Covance 6329-183 3M T-6295 PAGE: 403 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C92951 F2 519 75 TO 596 86 MASS(ES) AXILLARY REGION-RIGHT WEEK 64 603 87 TO 638 92 MASS(ES) AXILLARY REGION-RIGHT WEEK 64 645 93 TO 659 95 666 96 TO 708 102 MASS(ES) AXILLARY REGION-RIGHT WEEK 64 MASS(ES) AXILLARY REGION-RIGHT WEEK 64 715 103 MASS(ES) TO 715 103 AXILLARY REGION-RIGHT WEEK 64 442 64 MASS(ES) INGUINAL REGION-LEFT WEEK 64 449 65 TO 456 66 MASS(ES) INGUINAL REGION-LEFT WEEK 64 463 67 TO 484 70 MASS(ES) INGUINAL REGION-LEFT WEEK 64 20 X 20MM 30 X 30MM 40 X 30MM 50 X 35MM 50 X 50MM 25 X 20MM 15 X15MM 10 X 10MM 949 Covance 6329-183 3M T-6295 PAGE: 404 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 71 MASS(ES) 72 INGUINAL REGION-LEFT WEEK 64 73 MASS(ES) 74 INGUINAL REGION-LEFT WEEK 64 75 MASS(ES) INGUINAL REGION-LEFT WEEK 64 81 MASS(ES) 82 VENTRAL-ABDOMINAL-RIGHT WEEK 80 83 MASS(ES) 84 VENTRAL-ABDOMINAL-RIGHT WEEK 80 85 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 80 86 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 80 87 MASS(ES) 91 VENTRAL-ABDOMINAL-RIGHT WEEK 80 20 X 20MM 10 X 10 20 X 20MM 10 X 10MM 20 X 15MM 30 X 30MM 25 X 35MM 35 X 35MM 950 Covance 6329-183 3M T-6295 PAGE: 405 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C92951 C92952 C92954 F2 638 92 TO 659 95 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 80 666 96 TO 680 98 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 80 687 99 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 80 694 100 MASS(ES) TO 715 103 VENTRAL-ABDOMINAL-RIGHT WEEK 80 F2 435 63 MASS(ES) AXILLARY REGION-LEFT WEEK 63 442 64 TO 456 66 MASS(ES) AXILLARY REGION-LEFT WEEK 63 F2 526 76 TO 575 83 MASS(ES) INGUINAL REGION-LEFT WEEK 76 533 77 TO 554 80 MASS(ES) VENTRAL-CERVICAL-RIGHT WEEK 77 45 X 40MM 50 X 40MM 69 X 45MM 60 X 45MM 10 X 10MM 20 X 20MM 10 X 10MM 30 X 25MM 951 Covance 6329-183 3M T-6295 PAGE: 406 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C92954 C92955 F2 561 81 TO 589 85 MASS(ES) VENTRAL-CERVICAL-RIGHT WEEK 77 596 86 TO 645 93 MASS(ES) VENTRAL-CERVICAL-RIGHT WEEK 77 652 94 MASS(ES) TO 704 101 VENTRAL-CERVICAL-RIGHT WEEK 77 F2 519 75 TO 540 78 MASS(ES) INGUINAL REGION-LEFT WEEK 75 547 79 MASS(ES) INGUINAL REGION-LEFT WEEK 75 554 80 TO 575 83 MASS(ES) INGUINAL REGION-LEFT WEEK 75 582 84 TO 596 86 MASS(ES) INGUINAL REGION-LEFT WEEK 75 603 87 MASS(ES) INGUINAL REGION-LEFT WEEK 75 35 X 30MM 35 X 40MM 40 X 50MM 25 X 10MM 25 X 20MM 30 X 25MM 45 X 35MM 60 X 40MM 952 Covance 6329-183 3M T-6295 PAGE: 407 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 88 MASS(ES) 92 INGUINAL REGION-LEFT WEEK 75 83 MASS(ES) INGUINAL REGION-RIGHT WEEK 83 84 MASS(ES) INGUINAL REGION-RIGHT WEEK 83 85 MASS(ES) 92 INGUINAL REGION-RIGHT WEEK 83 62 MASS(ES) VENTRAL-ABDOMINAL WEEK 62 84 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 84 85 MASS(ES) 87 VENTRAL-ABDOMINAL-RIGHT WEEK 84 88 MASS(ES) 92 VENTRAL-ABDOMINAL-RIGHT WEEK 84 40 X 40MM 10 X 10MM 15 X 15MM 20 X 20MM 25 X 25MM 10 X 10MM 15 X 15MM 25 X 25MM 953 Covance 6329-183 3M T-6295 PAGE: 408 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 90 MASS(ES) HEAD-MAXILLARY-LEFT WEEK 90 82 MASS(ES) INGUINAL REGION-LEFT WEEK 82 83 MASS(ES) 85 INGUINAL REGION-LEFT WEEK 82 86 MASS(ES) INGUINAL REGION-LEFT WEEK 82 87 MASS(ES) 91 INGUINAL REGION-LEFT WEEK 82 92 MASS(ES) 93 INGUINAL REGION-LEFT WEEK 82 94 MASS(ES) 95 INGUINAL REGION-LEFT WEEK 82 96 MASS(ES) 97 INGUINAL REGION-LEFT WEEK 82 10 X 10MM 10 X 10MM 15 X 15MM 25 X 15MM 25 X 25MM 35 X 35MM 40 X 40MM 40 X 50MM 954 Covance 6329-183 3M T-6295 PAGE: 409 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C92956 C92957 F2 680 98 MASS(ES) TO 694 100 INGUINAL REGION-LEFT WEEK 82 701 101 MASS(ES) TO 709 102 INGUINAL REGION-LEFT WEEK 82 F2 435 63 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 63 442 64 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 63 449 65 TO 463 67 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 63 470 68 TO 477 69 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 63 484 70 TO 505 73 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 63 512 74 TO 554 80 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 63 50 X 60MM 40 X 50MM 10 X 10MM 15 X 15MM 20 X 20MM 35 X 30MM 40 X 35MM 60 X 50MM 955 Covance 6329-183 3M T-6295 PAGE: 410 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C92957 C92960 F2 561 81 TO 589 85 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 63 596 86 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 63 603 87 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 63 610 88 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 63 617 89 MASS(ES) TO 733 105 VENTRAL-ABDOMINAL-LEFT WEEK 63 F2 498 72 TO 505 73 MASS(ES) INGUINAL REGION-LEFT WEEK 72 512 74 TO 519 75 MASS(ES) INGUINAL REGION-LEFT WEEK 72 526 76 TO 540 78 MASS(ES) INGUINAL REGION-LEFT WEEK 72 80 X 65MM 95 X 65MM 95 X 85MM 95 X 70MM 100 X 80MM 25 X 25MM 40 X 40MM 50 X 40MM 956 Covance 6329-183 3M T-6295 PAGE: 411 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT F2 547 79 TO 554 80 MASS(ES) INGUINAL REGION-LEFT WEEK 72 561 81 TO 575 83 MASS(ES) INGUINAL REGION-LEFT WEEK 72 582 84 TO 596 86 MASS(ES) INGUINAL REGION-LEFT WEEK 72 603 87 MASS(ES) INGUINAL REGION-LEFT WEEK 72 CO CO 610 MASS(ES) INGUINAL REGION-LEFT WEEK 72 617 89 TO 624 90 MASS(ES) INGUINAL REGION-LEFT WEEK 72 631 91 TO 659 95 MASS(ES) INGUINAL REGION-LEFT WEEK 72 666 96 MASS(ES) TO 701 101 INGUINAL REGION-LEFT WEEK 72 50 X 50MM 70 X 60MM 80 X 60MM 120 X 80MM 105 X 70MM 110 X 80MM 100 X 70MM 110 X 70MM 957 Covance 6329-183 3M T-6295 PAGE: 412 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C92960 C92961 F2 708 102 MASS(ES) TO 715 103 INGUINAL REGION-LEFT WEEK 72 722 104 MASS(ES) TO 733 105 INGUINAL REGION-LEFT WEEK 72 F2 526 76 MASS(ES) INGUINAL REGION-LEFT WEEK 76 533 77 TO 554 80 MASS(ES) INGUINAL REGION-LEFT WEEK 76 561 81 TO 582 84 MASS(ES) INGUINAL REGION-LEFT WEEK 76 589 85 TO 596 86 MASS(ES) INGUINAL REGION-LEFT WEEK 76 603 87 MASS(ES) INGUINAL REGION-LEFT WEEK 76 610 88 MASS(ES) INGUINAL REGION-LEFT WEEK 76 120 X 70MM 120 X 80MM 10 X 10MM 20 X 20MM 35 X 35MM 50 X 40MM 80 X 60MM 70 X 50MM 958 Covance 6329-183 3M T-6295 PAGE: 413 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 89 MASS(ES) 93 INGUINAL REGION-LEFT WEEK 76 94 MASS(ES) 95 INGUINAL REGION-LEFT WEEK 76 96 MASS(ES) 97 INGUINAL REGION-LEFT WEEK 76 98 MASS(ES) INGUINAL REGION-LEFT WEEK 76 99 MASS(ES) 99 INGUINAL REGION-LEFT WEEK 76 72 MASS(ES) AXILLARY REGION-LEFT WEEK 72 73 MASS(ES) 74 AXILLARY REGION-LEFT WEEK 72 75 MASS(ES) AXILLARY REGION-LEFT WEEK 72 70 X 70MM 90 X 70MM 100 X 70MM 110 X 70MM 120 X 70MM 10 X 10MM 10 X 15MM 15 X 15MM 959 Covance 6329-183 3M T-6295 PAGE: 414 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 76 MASS(ES) AXILLARY REGION-LEFT WEEK 72 77 MASS(ES) 78 AXILLARY REGION-LEFT WEEK 72 79 MASS(ES) 80 AXILLARY REGION-LEFT WEEK 72 81 MASS(ES) 86 AXILLARY REGION-LEFT WEEK 72 87 MASS(ES) 94 AXILLARY REGION-LEFT WEEK 72 75 MASS(ES) 78 INGUINAL REGION-RIGHT WEEK 75 79 MASS(ES) 84 INGUINAL REGION-RIGHT WEEK 75 85 MASS(ES) 94 INGUINAL REGION-RIGHT WEEK 75 30 X 30MM 20 X 20MM 20 X 25MM 30 X 30MM 30 X 30MM 10 X 10MM 15 X 15MM 20 X 20MM 960 Covance 6329-183 3M T-6295 PAGE: 415 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 84 MASS(ES) 86 VENTRAL-ABDOMINAL-LEFT WEEK 83 87 MASS(ES) 93 VENTRAL-ABDOMINAL-LEFT WEEK 83 94 MASS(ES) 94 VENTRAL-ABDOMINAL-LEFT WEEK 83 68 MASS(ES) 71 VENTRAL-ABDOMINAL-RIGHT WEEK 68 72 MASS(ES) 73 VENTRAL-ABDOMINAL-RIGHT WEEK 68 74 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 68 75 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 68 76 MASS(ES) 78 VENTRAL-ABDOMINAL-RIGHT WEEK 68 15 X 15MM 10 X 10MM 15 X 15MM 20 X 20MM 30 X 30MM 40 X 35MM 25 X 30MM 30 X 30MM 961 Covance 6329-183 3M T-6295 PAGE: 416 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 79 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 68 80 MASS(ES) 83 VENTRAL-ABDOMINAL-RIGHT WEEK 68 84 MASS(ES) 86 VENTRAL-ABDOMINAL-RIGHT WEEK 68 87 MASS(ES) 90 VENTRAL-ABDOMINAL-RIGHT WEEK 68 91 MASS(ES) 93 VENTRAL-ABDOMINAL-RIGHT WEEK 68 94 MASS(ES) 94 VENTRAL-ABDOMINAL-RIGHT WEEK 68 84 MASS(ES) VENTRAL-CERVICAL WEEK 83 85 MASS(ES) 87 VENTRAL-CERVICAL WEEK 83 30 X 40MM 40 X 40MM 50 X 50MM 65 X 75MM 50 X 50MM 60 X 50MM 10 X 10MM 20 X 20MM 962 Covance 6329-183 3M T-6295 PAGE: 417 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C92964 C92965 F2 610 88 TO 631 91 MASS(ES) VENTRAL-CERVICAL WEEK 83 638 92 MASS(ES) TO 701 101 VENTRAL-CERVICAL WEEK 83 708 102 MASS(ES) VENTRAL-CERVICAL WEEK 83 715 103 MASS(ES) TO 731 105 VENTRAL-CERVICAL WEEK 83 F2 414 60 TO 442 64 MASS(ES) VENTRAL-ABDOMINAL WEEK 60 449 65 TO 463 67 MASS(ES) VENTRAL-ABDOMINAL WEEK 60 470 68 TO 505 73 MASS(ES) VENTRAL-ABDOMINAL WEEK 60 512 74 MASS(ES) VENTRAL-ABDOMINAL WEEK 60 10 X 10MM 15 X 15MM 20 X 20MM 30 X 30MM 20 X 20MM 30 X 30MM 45 X 45MM 65 X 60MM 963 Covance 6329-183 3M T-6295 PAGE: 418 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C92965 C92971 F2 519 75 526 76 TO 573 82 F2 463 67 470 68 TO 505 73 512 74 TO 554 80 561 81 TO 589 85 596 86 603 87 TO 624 90 MASS(ES) VENTRAL-ABDOMINAL WEEK 60 MASS(ES) VENTRAL-ABDOMINAL WEEK 60 MASS(ES) INGUINAL REGION-LEFT WEEK 67 MASS(ES) INGUINAL REGION-LEFT WEEK 67 MASS(ES) INGUINAL REGION-LEFT WEEK 67 MASS(ES) INGUINAL REGION-LEFT WEEK 67 MASS(ES) INGUINAL REGION-LEFT WEEK 67 MASS(ES) INGUINAL REGION-LEFT WEEK 67 45 X 50MM 45 X 60MM 35 X 35MM 50 X 50MM 70 X 60MM 95 X 80MM 100 X 90MM 110 X 110MM 964 Covance 6329-183 3M T-6295 PAGE: 419 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 72 MASS(ES) 74 SHOULDER-RIGHT WEEK 72 75 MASS(ES) SHOULDER-RIGHT WEEK 72 76 MASS(ES) 78 SHOULDER-RIGHT WEEK 72 79 MASS(ES) 80 SHOULDER-RIGHT WEEK 72 81 MASS(ES) 88 SHOULDER-RIGHT WEEK 72 89 MASS(ES) 91 SHOULDER-RIGHT WEEK 72 92 MASS(ES) 96 SHOULDER-RIGHT WEEK 72 89 MASS(ES) 95 VENTRAL-ABDOMINAL WEEK 89 15 X 15MM 25 X 25MM 30 X 25MM 30 X 30MM 45 X 50MM 55 X 60MM 55 X 40MM 10 X 10MM 965 Covance 6329-183 3M T-6295 PAGE: 420 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C92975 C92976 F2 666 96 MASS(ES) VENTRAL-ABDOMINAL WEEK 89 673 97 MASS(ES) VENTRAL-ABDOMINAL WEEK 89 680 98 MASS(ES) TO 701 101 VENTRAL-ABDOMINAL WEEK 89 708 102 MASS(ES) TO 715 103 VENTRAL-ABDOMINAL WEEK 89 722 104 MASS(ES) TO 733 105 VENTRAL-ABDOMINAL WEEK 89 323 47 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 47 F2 575 83 TO 596 86 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 83 603 87 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 83 30 X 30MM 40 X 35MM 60 X 40MM 60 X 50MM 70 X 70MM 15 X 10MM 10 X 10MM 35 X 25MM 966 Covance 6329-183 3M T-6295 PAGE: 421 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C92976 C92979 F2 610 88 TO 631 91 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 83 638 92 TO 645 93 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 83 652 94 TO 666 96 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 83 673 97 MASS(ES) TO 701 101 VENTRAL-ABDOMINAL-LEFT WEEK 83 708 102 MASS(ES) TO 712 102 VENTRAL-ABDOMINAL-LEFT WEEK 83 610 88 TO 631 91 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 88 638 92 MASS(ES) TO 701 101 VENTRAL-ABDOMINAL-LEFT WEEK 88 F2 561 81 MASS(ES) AXILLARY REGION-RIGHT WEEK 80 20 X 25MM POSTERIOR 35 X 20MM POSTERIOR 40 X 20MM POSTERIOR 40 X 30MM POSTERIOR 50 X 50MM TWO MASSES APPEAR TO HAVE GROWN TOGETHER 20 X 20MM 25 X 20MM 10 X 10MM Covance 6329-183 3M T-6295 PAGE: 422 967 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 82 MASS(ES) 83 AXILLARY REGION-RIGHT WEEK 80 84 MASS(ES) 85 AXILLARY REGION-RIGHT WEEK 80 86 MASS(ES) AXILLARY REGION-RIGHT WEEK 80 87 MASS(ES) AXILLARY REGION-RIGHT WEEK 80 88 MASS(ES) 89 AXILLARY REGION-RIGHT WEEK 80 41 MASS(ES) AXILLARY REGION-LEFT WEEK 38 42 MASS(ES) AXILLARY REGION-LEFT WEEK 38 43 MASS(ES) 46 AXILLARY REGION-LEFT WEEK 38 20 X 20MM 25 X 30MM 25 X 35MM 30 X 30MM 40 X 35MM 10 X 10MM 15 X 20MM 20 X 20MM 968 Covance 6329-183 3M T-6295 PAGE: 423 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 47 MASS(ES) 50 AXILLARY REGION-LEFT WEEK 38 51 MASS(ES) AXILLARY REGION-LEFT WEEK 38 52 MASS(ES) AXILLARY REGION-LEFT WEEK 38 53 MASS(ES) 55 AXILLARY REGION-LEFT WEEK 38 56 MASS(ES) 57 AXILLARY REGION-LEFT WEEK 38 58 MASS(ES) 61 AXILLARY REGION-LEFT WEEK 38 62 MASS(ES) 63 AXILLARY REGION-LEFT WEEK 38 57 MASS(ES) 59 LIMB-HIND-RIGHT WEEK 55 20 X 25MM 20 X 30MM 30 X 30MM 35 X 40MM 25 X 30MM 20 X 25MM 30 X 25MM 40 X 40MM 969 Covance 6329-183 3M T-6295 PAGE: 424 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C92980 C92983 C92984 F2 414 60 TO 421 61 MASS(ES) LIMB-HIND-RIGHT WEEK 55 428 62 TO 435 63 MASS(ES) LIMB-HIND-RIGHT WEEK 55 F2 652 94 TO 680 98 MASS(ES) INGUINAL REGION-LEFT WEEK 94 687 99 MASS(ES) INGUINAL REGION-LEFT WEEK 94 694 100 MASS(ES) INGUINAL REGION-LEFT WEEK 94 701 101 MASS(ES) INGUINAL REGION-LEFT WEEK 94 708 102 MASS(ES) TO 731 105 INGUINAL REGION-LEFT WEEK 94 F2 645 93 TO 673 97 MASS(ES) AXILLARY REGION-LEFT WEEK 93 50 X 45MM 60 X 55MM 30 X 20MM 40 X 30MM 40 X 45MM 45 X 30MM 45 X 50MM 10 X 10MM 970 Covance 6329-183 3M T-6295 PAGE: 425 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C92984 F2 680 98 MASS(ES) TO 694 100 AXILLARY REGION-LEFT WEEK 93 701 101 MASS(ES) TO 732 105 AXILLARY REGION-LEFT WEEK 93 568 82 TO 575 83 MASS(ES) INGUINAL REGION-RIGHT WEEK 75 582 84 MASS(ES) INGUINAL REGION-RIGHT WEEK 75 589 85 TO 603 87 MASS(ES) INGUINAL REGION-RIGHT WEEK 75 610 88 MASS(ES) INGUINAL REGION-RIGHT WEEK 75 617 89 TO 645 93 MASS(ES) INGUINAL REGION-RIGHT WEEK 75 652 94 TO 687 99 MASS(ES) INGUINAL REGION-RIGHT WEEK 75 20 X 15MM 15 X 10MM 10 X 10MM 15 X 15MM 20 X 20MM 25 X 25MM 45 X 25MM 50 X 30MM 971 Covance 6329-183 3M T-6295 PAGE: 426 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C92984 F2 694 100 MASS(ES) TO 732 105 INGUINAL REGION-RIGHT WEEK 75 414 60 TO 456 66 MASS(ES) VENTRAL-ABDOMINAL WEEK 60 722 104 MASS(ES) VENTRAL-ABDOMINAL WEEK 104 729 105 MASS(ES) TO 732 105 VENTRAL-ABDOMINAL WEEK 104 533 77 TO 575 83 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 75 582 84 TO 603 87 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 75 610 88 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 75 617 89 TO 680 98 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 75 50 X 40MM 10 X 10MM 10 X 10MM 15 X 15MM 15 X 15MM 20 X 25MM 25 X 30MM 45 X 30MM 972 Covance 6329-183 3M T-6295 PAGE: 427 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C92984 C92985 F2 687 99 MASS(ES) TO 732 105 VENTRAL-ABDOMINAL-RIGHT WEEK 75 F2 526 76 TO 540 78 MASS(ES) AXILLARY REGION-RIGHT WEEK 76 547 79 MASS(ES) AXILLARY REGION-RIGHT WEEK 76 554 80 MASS(ES) AXILLARY REGION-RIGHT WEEK 76 561 81 TO 582 84 MASS(ES) AXILLARY REGION-RIGHT WEEK 76 589 85 TO 596 86 MASS(ES) AXILLARY REGION-RIGHT WEEK 76 603 87 TO 638 92 MASS(ES) AXILLARY REGION-RIGHT WEEK 76 645 93 TO 659 95 MASS(ES) AXILLARY REGION-RIGHT WEEK 76 50 X 40MM 15 X 15MM 20 X 20MM 25 X 25MM 45 X 35MM 50 X 50MM 65 X 65MM 80 X 65MM 973 Covance 6329-183 3M T-6295 PAGE: 428 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 96 MASS(ES) 98 AXILLARY REGION-RIGHT WEEK 76 96 MASS(ES) 98 INGUINAL REGION-RIGHT WEEK 96 82 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 82 83 MASS(ES) 84 VENTRAL-ABDOMINAL-LEFT WEEK 82 85 MASS(ES) 87 VENTRAL-ABDOMINAL-LEFT WEEK 82 88 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 82 89 MASS(ES) 90 VENTRAL-ABDOMINAL-LEFT WEEK 82 91 MASS(ES) 98 VENTRAL-ABDOMINAL-LEFT WEEK 82 80 X 80MM 25 X 25MM 10 X 10MM 15 X 15MM 20 X 20MM 25 X 25MM 30 X 30MM 20 X 20MM 974 Covance 6329-183 3M T-6295 PAGE: 429 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C92985 C92986 F2 659 95 TO 673 97 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 95 680 98 TO 680 98 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 95 659 95 TO 680 98 MASS(ES) VENTRAL-THORACIC-LEFT WEEK 95 F2 589 85 TO 610 88 MASS(ES) INGUINAL REGION-LEFT WEEK 85 617 89 TO 638 92 MASS(ES) INGUINAL REGION-LEFT WEEK 85 645 93 MASS(ES) INGUINAL REGION-LEFT WEEK 85 652 94 TO 673 97 MASS(ES) INGUINAL REGION-LEFT WEEK 85 680 98 MASS(ES) TO 733 105 INGUINAL REGION-LEFT WEEK 85 15 X 15MM 20 X 20MM 10 X 10MM 40 X 25MM 50 X 40MM 55 X 45MM 60 X 50MM 70 X 60MM 975 Covance 6329-183 3M T-6295 PAGE: 430 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C92986 C92990 F2 526 76 TO 554 80 MASS(ES) INGUINAL REGION-RIGHT WEEK 76 694 100 MASS(ES) TO 715 103 INGUINAL REGION-RIGHT WEEK 100 F2 505 73 TO 512 74 MASS(ES) AXILLARY REGION-LEFT WEEK 73 519 75 MASS(ES) AXILLARY REGION-LEFT WEEK 73 526 76 MASS(ES) AXILLARY REGION-LEFT WEEK 73 533 77 TO 547 79 MASS(ES) AXILLARY REGION-LEFT WEEK 73 554 80 MASS(ES) AXILLARY REGION-LEFT WEEK 73 561 81 TO 568 82 MASS(ES) AXILLARY REGION-LEFT WEEK 73 10 X 10MM 20 X 20MM 10 X 10MM 20 X 10MM 20 X 15MM 30 X 25MM 35 X 30MM 40 X 40MM 976 Covance 6329-183 3M T-6295 PAGE: 431 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 83 MASS(ES) AXILLARY REGION-LEFT WEEK 73 84 MASS(ES) 86 AXILLARY REGION-LEFT WEEK 73 87 MASS(ES) AXILLARY REGION-LEFT WEEK 73 88 MASS(ES) AXILLARY REGION-LEFT WEEK 73 89 MASS(ES) 94 AXILLARY REGION-LEFT WEEK 73 63 MASS(ES) 66 VENTRAL-ABDOMINAL WEEK 63 89 MASS(ES) 90 VENTRAL-CERVICAL-RIGHT WEEK 89 91 MASS(ES) VENTRAL-CERVICAL-RIGHT WEEK 89 50 X 30MM 50 X 35MM 65 X 50MM 50 X 45MM 65 X 55MM 10 X 10MM 35 X 35MM 35 X 60MM 977 Covance 6329-183 3M T-6295 PAGE: 432 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C92990 F2 638 92 TO 645 93 652 94 TO 653 94 MASS(ES) VENTRAL-CERVICAL-RIGHT WEEK 89 MASS(ES) VENTRAL-CERVICAL-RIGHT WEEK 89 50 X 60MM 50 X 90MM Covance 6329-183 3M T-6295 PAGE: 433 978 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 434 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F3 DOSE: 2 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92991 T 103 APPEARANCE MALOCCLUSION SKIN & PELAGE SORE/SCAB PAW-HIND-RIGHT NORMAL NO REMARKABLE OBSERVATIONS C92992 M MASS(ES) AXILLARY REGION-LEFT WEEK 44 AXILLARY REGION-RIGHT WEEK 53 84 EXCRETION FEW FECES EYES SQUINTED PERI-ORBITAL-RIGHT 407, 414, 421, 428, 435, 442, 449, 456, 463, 470, 477, 484, 491 498, 505, 512, 519, 526, 533, 540, 547, 554, 561, 568, 575, 582 589, 596, 603, 610, 617, 624, 631, 638, 645, 652, 659, 666, 673 680, 687, 694, 701, 708, 715, 719 547, 554 561, 568, 575, 582, 589, 596, 603, 610, 617, 624, 631 638, 645 652, 659, 666, 673, 680, 687, 694, 701, 708, 715, 719 1 , 8 , 15 113, 120 204, 211 295, 337 2 2 , 29, 36 , 43,, 50, 57, 64, 71, 78, 85, 92, 99, 106 127,, 134, 141, 148, 155, 162 , 16 9, 176, 183,. 190, 197 218, 225, 232, 239, 246, 253 , 26 0, 267, 274, 281, 288 344, 351, 358 302C, 30 , 316C, 323C, 330C 365, 372 379, 386, 393, 400, 407 578 578, 582-583 979 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 435 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F3 (CONTINUED FROM PREVIOUS PAGE) C92992 M 84 SKIN & PELAGE SORE/SCAB DORSAL-CERVICAL DORSAL-THORACIC-RIGHT NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) AXILLARY REGION-LEFT WEEK 78 DORSAL WEEK 76 INGUINAL REGION-LEFT WEEK 46 WEEK 81 INGUINAL REGION-RIGHT WEEK 76 VENTRAL-CERVICAL WEEK 79 VENTRAL-THORACIC WEEK 62 DOSE: 2 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 547, 554, 561, 568 , 575, 578 505 1 , 8 , 15, 22, 29, 36, 43 , 50 , 57, 64, 71 , 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148 , 155, 162, 169, 176, 183,, 190, 197 204, 211, 218, 225 , 232, 239 , 246, 253, 260, 267, 274, 281, 288 295, 302, 309 54 0C, 547C, 554C, 561C, 568C , 575C , 578C , 582-583C 526C, 533C, 540C, 54 7C, 554C , 561C , 568C , 575C, 578C, 582-583C 316C, 323C, 330C, 337C, 344C , 351C , 358C , 365C, 372C, 379C, 386C 393C, 400C, 407C, 414C, 421C 561C, 568C, 575C, 578C, 582- 583C 526C, 533C, 540C, 54 7C, 554C , 561C , 568C , 575C, 578C, 582-583C 54 7C, 554C, 561C, 568C, 575C , 578C , 582- 583C 428C, 435C, 442C, 44 9C, 456C , 463C , 470C , 477C, 484C, 491C, 498C 505C, 512C, 519C, 526C, 533C , 540C , 547C , 554C, 561C, 568C, 575C 578C, 582-583C 980 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 436 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F3 DOSE: 2 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92993 2 C92994 M 14 NORMAL NO REMARKABLE OBSERVATIONS 82 APPEARANCE MALOCCLUSION MISSING TEETH THIN EXCRETION NO FECES EYES DISCOLORED/LIGHT RED EYE-LEFT RED DISCHARGE EYE-LEFT RESPIRATION AUDIBLE SKIN & PELAGE YELLOW HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS 1, 8 , 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92-93 141 134 568, 572 572 519, 526, 547, 554, 561, 568, 572 498, 505, 512, 519, 526, 533, 540, 547, 554, 561, 568, 572 260 572 1 , 8 , 15 113, 120 218, 225 316, 323 407, 414 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 127, 148 , 155, 162, 169, 176 , 183, 190, 197, 204 , 211 232, 239 , 246, 253, 267, 274 , 281, 288, 295, 302 , 309 330, 337 , 344, 351, 358, 365 , 372, 379, 386, 393 , 400 421, 428 , 435, 442, 449, 456 , 463, 470, 477, 484 , 491 981 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 437 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F3 DOSE: 2 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92995 M C92996 1 C92997 M 9 APPEARANCE MISSING TAIL-DISTAL SWOLLEN TAIL-DISTAL SKIN & PELAGE PALE ENTIRE BODY SORE/SCAB TAIL NORMAL NO REMARKABLE OBSERVATIONS 4 NORMAL NO REMARKABLE OBSERVATIONS 83 BEHAVIOR HYPOACTIVE <UNSPECIFIED> RECUMBENT LATERAL EYES RED DISCHARGE EYES SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE 32, 36, 43, 50, 57, 59 32, 36, 43, 50, 57, 59 57, 59 29, 32, 36, 43, 50, 57, 59 1, 8 , 15, 22 1, 8 , 15, 22 575 575 575 519, 526, 533, 540, 547, 554, 561, 568 982 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 438 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F3 (CONTINUED FROM PREVIOUS PAGE) C92997 M 83 NORMAL NO REMARKABLE OBSERVATIONS C92998 M MASS(ES) INGUINAL REGION-LEFT WEEK 73 VENTRAL-ABDOMINAL-LEFT WEEK 50 VENTRAL-ABDOMINAL-RIGHT WEEK 74 VENTRAL-CERVICAL WEEK 76 46 APPEARANCE THIN BEHAVIOR ATAXIC <UNSPECIFIED> HYPOACTIVE <UNSPECIFIED> MILD EYES SQUINTED PERI-ORBITAL DOSE: 2 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 1 , 8 ,r 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 113, 1 2 0 , 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 204, 2 1 1 , 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 295, 302, 309, 316 , 323, 330, 337, 400 , 407, 414, 421, 428 442, 449, 456, 463 , 470, 477, 484, 491 , 498 505C, 512C, 519C, 526C, 533C, 54 0C, 54 7C, 554C, 561C, 568C 344, 351, 358, 36 5, 372, 379, 386, 393 512C, 519C, 526C, 533C, 54 0C, 54 7C, 554C, 561C, 568C, 575C 526C, 533C, 54 0C, 54 7C, 554C, 561C, 568C 288, 295, 302, 309, 316 316 295, 302, 309, 316 274 316 983 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 439 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F3 (CONTINUED FROM PREVIOUS PAGE) C92998 M 46 RESPIRATION AUDIBLE SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE YELLOW HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS C92999 D 92 APPEARANCE HEAD TILT LEFT MALOCCLUSION THIN EYES CLEAR DISCHARGE EYE-RIGHT PROTRUDING EYES OTHER SKIN & PELAGE RED SKIN EARS NORMAL NO REMARKABLE OBSERVATIONS DOSE: 2 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 274 274, 281, 288 316 1 , 8 , 15, 22, 29, 36, 43,, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127, 134, 141, 148, 155, 162 , 169, 176, 183,. 190, 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253 , 260, 267 561, 568, 575, 582, 589, 596, 603, 610, 617, 624, 631, 638, 643 631 596, 603, 610, 617, 624, 631, 638, 643 547, 554 505, 512, 519 400C, 407C, 414C 547, 554 1, 8 , 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 984 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 440 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F3 (CONTINUED FROM PREVIOUS PAGE) C92999 D 92 NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) AXILLARY REGION-LEFT WEEK 79 AXILLARY REGION-RIGHT WEEK 56 VENTRAL-ABDOMINAL WEEK 59 C93000 2 C93001 M VENTRAL-ABDOMINAL-RIGHT WEEK 76 VENTRAL-CERVICAL WEEK 79 14 NORMAL NO REMARKABLE OBSERVATIONS 84 APPEARANCE HEAD TILT RIGHT BEHAVIOR ATAXIC <UNSPECIFIED> DOSE: 2 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 113, 1 2 0 , 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 547, 554, 561 386, 393, 400, 407 , 414, 421 407C, 414C , 421C, 428C, 435C, 442C, 44 9C, 456C, 463C, 470C, 477C 484C, 491C , 498C, 505C, 512C, 519C, 526C, 533C, 54 0C, 54 7C, 554C 561C, 568C , 575C, 582C, 58 9C, 596C, 603C, 610C, 617C, 624C, 631C 638C, 643C 526C, 533C , 540C, 54 7C, 554C, 561C, 568C 54 7C, 554C , 561C, 568C 1, 8 , 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92-93 575, 582, 584 584 985 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 441 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F3 (CONTINUED FROM PREVIOUS PAGE) C93001 M 84 EXCRETION FEW FECES EYES RED DISCHARGE EYES SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) INGUINAL REGION-LEFT WEEK 82 VENTRAL-ABDOMINAL-LEFT WEEK 76 DOSE: 2 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 584 584 575 1 , 8 ,r 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407 , 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498 , 505, 512, 519 56 8C, 575C, 582C, 584C 526, 533, 540C, 547C, 554C, 561C, 568C, 575C, 582C, 584C 986 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 442 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F3 DOSE: 2 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C93002 M 81 APPEARANCE THIN DISCHARGE NASAL RED IN COLOR EYES RED DISCHARGE EYES RESPIRATION IRREGULAR LABORED SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) AXILLARY REGION-LEFT WEEK 70 AXILLARY REGION-RIGHT WEEK 58 561 547 526, 533, 561 561 561 561 1 , 8 ,r 15, 2 2 , 29, 36, 43,, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127, 134 , 141, 148, 155,. 162, 169, 176, 183,. 190, 197 204, 2 1 1 , 218, 225 , 232, 239, 246,. 253, 260, 267, 274, 281, 288 295, 302, 309, 316 , 323 484C, 491C, 498C, 505C, 512C, 519C, 526C, 533C, 540C, 547C, 554C 561C 400, 407, 414, 421 987 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 443 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F3 (CONTINUED FROM PREVIOUS PAGE) C93002 M 81 MASS(ES) INGUINAL REGION-LEFT WEEK 48 INGUINAL REGION-RIGHT WEEK 56 C93003 T VENTRAL-ABDOMINAL WEEK 54 103 APPEARANCE MALOCCLUSION EXCRETION FEW FECES SKIN & PELAGE SORE/SCAB AXILLARY REGION-LEFT YELLOW HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS DOSE: 2 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 330C , 337C, 344C, 351C, 358C, 365C, 372C, 379C 386C , 393C, 400C, 407C, 414C, 421C, 428C, 435C, 442C, 44 9C, 456C 463C , 470C, 477C, 484C, 491C, 498C, 505C, 512C, 519C, 526C, 533C 540C , 547C, 554C, 561C 372C , 379C, 386C, 393C 85, 92, 99, 106, 113, 120, 127 , 129 , 134, 141, 148, 155, 162 211169, 176, 183, 190 , 197, 204, , 218, 225, 232 , 239,. 246, 253 260, 267, 274, 281 , 288, 295, 302, 309, 316, 323 , 330, 337, 344 351, 358, 365, 372 , 379,. 386, 393, 400, 407, 414 , 421,. 428, 435 442, 449, 456, 463 , 470, 477, 484, 491, 498, 505 , 512, 519, 526 533, 540, 547, 554 , 561,. 568, 575, 582, 589, 596 , 603,. 610, 617 666624, 631, 638, 645 , 652, 659, , 673, 680, 687 , 694, 701, 708 715, 719 687, 694, 701, 708 715, 719 715, 719 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78 988 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 444 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F3 (CONTINUED FROM PREVIOUS PAGE) C93003 T 103 MASS(ES) AXILLARY REGION-LEFT WEEK 56 C93004 M 75 BEHAVIOR ATAXIC <UNSPECIFIED> HYPOACTIVE <UNSPECIFIED> EXCRETION FEW FECES EYES SQUINTED PERI-ORBITAL-RIGHT NORMAL NO REMARKABLE OBSERVATIONS DOSE: 2 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 386C, 463C, 54 0C, 617C, 694C, 3 93C, 4 7 0C, 54 7C, 624C, 7 01C, 400C, 477C, 554C, 631C, 7 08C, 407C, 484C, 561C, 638C, 715C, 414C, 491C, 56 8C, 64 5C, 719C 421C, 498C, 575C, 652C, 42 8C, 5 05C, 582C, 6 5 9C, 435C, 512C, 58 9C, 666C, 442C, 519C, 5 96C, 673C, 44 9C, 52 6C, 603C, 680C, 456C 533C 610C 687C 520 520 507, 512 505, 507, 512, 519 -520 1 , 8 ,r 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407 , 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498 989 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 445 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F3 DOSE: 2 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C93005 M 90 APPEARANCE THIN BEHAVIOR ATAXIC <UNSPECIFIED> HYPOACTIVE <UNSPECIFIED> EXCRETION FEW FECES EYES RED DISCHARGE EYE-LEFT EYES SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE HEAD-CRANIAL NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) AXILLARY REGION-LEFT WEEK 51 575, 582, 589, 596, 603, 610, 617, 624 630 630 575, 630 540, 547, 554, 561, 568, 575 630 470, 477, 484, 547, 554, 561, 568 393, 400, 407 1 , 8 ,r 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127, 134 , 141, 148, 155,. 162, 169, 176, 183,. 190, 197 204, 2 1 1 , 218, 225 , 232, 239, 246,. 253, 260, 267, 274, 281, 288 295, 302, 309, 316 , 323, 330, 337, 344 351, 358, 365, 372, 379,, 386, 393, 400C, 407C, 414C, 421C, 428C 435C,. 442C, 449C, 456C, 463C, 470C, 477C, 484C,. 491C, 498C, 505C 990 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 446 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F3 (CONTINUED FROM PREVIOUS PAGE) C93005 M 90 MASS(ES) AXILLARY REGION-LEFT WEEK 51 C93006 1 INGUINAL REGION-RIGHT WEEK 79 4 RMAL NO REMARKABLE OBSERVATIONS C93007 T 103 RMAL NO REMARKABLE OBSERVATIONS C93008 1 WART LIKE LESION(S) TAIL WEEK 80 4 NORMAL NO REMARKABLE OBSERVATIONS DOSE: 2 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 512C, 519C , 526C, 533C, 54 0C, 547C , 554C , 561C, 568C, 575C , 582C 58 9C, 596C , 603C, 610C, 617C, 624C , 630C 54 7C, 554C , 561C, 568C 1 , 8 ,. 15, 22 1 , 8 ,. 15, 2 2 , 29, 36, 43 , 50, 57, 64, 71 , 78, 85 , 92, 99, 106 113, 1 2 0 , 127,. 134, 141, 148, 155, 162, 169, 176 , 183, 190 , 197 204, 2 1 1 , 218,. 225, 232, 239, 246, 253, 260, 267 , 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344, 351, 358 , 365, 372 , 379 386, 393, 400,. 407, 414, 421, 428, 435, 442, 449 , 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526, 533, 540 , 547, 561 , 568 575, 582, 589,. 596, 603, 610, 617, 624, 631, 638 , 645, 652 , 659 666, 673, 680,. 687, 694, 701, 708, 715, 719 554 1, 8 , 15, 22 991 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 447 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F3 DOSE: 2 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C93009 M 92 APPEARANCE MALOCCLUSION BEHAVIOR HYPOACTIVE <UNSPECIFIED> EYES DISCOLORED/LIGHT RED EYE-LEFT SCAB EYE-LEFT SQUINTED PERI-ORBITAL-RIGHT NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) HEAD-MAXILLARY-LEFT WEEK 91 VENTRAL-ABDOMINAL-RIGHT WEEK 76 WEEK 79 631, 638, 642 642 642 642 642 1 , 8 ,r 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407 , 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498 , 505, 512, 519, 533 , 540 631C, 638C, 642C 526 547, 554, 561C, 568C, 575C, 582C, 589C, 596C, 603C, 610, 617 624, 631C, 638C, 642C 992 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 448 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F3 DOSE: 2 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C93010 M 91 APPEARANCE HEAD TILT RIGHT THIN BEHAVIOR HYPOACTIVE <UNSPECIFIED> EXCRETION FEW FECES EYES RED DISCHARGE EYES SKIN & PELAGE BROWN HAIR COAT PERINEAL AREA NORMAL NO REMARKABLE OBSERVATIONS 631 631 631 617, 624 624, 631 624, 631 1 , 8 ,r 15, 2 2 , 29, 36 , 43,r 50, 113, 1 2 0 , 127,. 134, 141, 148, 204, 2 1 1 , 218, 225, 232, 239, 295, 302, 309,. 316, 323, 330, 386, 393, 400, 407, 414, 421, 477, 484, 491, 498, 505, 512, 568, 575, 582, 589, 596, 603, 57, 64, 71, 78, 85, 155, 162 , 169, 176, 246, 253 , 260, 267, 337, 344 , 351, 358, 428, 435 , 442, 449, 519, 526 , 533, 540, 610 92, 99, 106 183, 190 , 197 274, 281 , 288 365, 372 , 379 456, 463 , 470 547, 554 , 561 993 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 449 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F3 DOSE: 2 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C93011 T 103 SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE NORMAL NO REMARKABLE OBSERVATIONS M 81 APPEARANCE THIN EYES CLEAR DISCHARGE EYE-LEFT SKIN & PELAGE BLACK SKIN VENTRAL-THORACIC-LEFT SORE/SCAB DORSAL-CERVICAL-RIGHT HEAD-MAXILLARY-RIGHT VENTRAL-THORACIC-LEFT NORMAL NO REMARKABLE OBSERVATIONS 519, 526, 533, 540 1 , 8 ,r 15, 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127, 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 547, 554 , 561, 568, 575, 582 , 589 596, 603, 610, 617, 624, 631, 638, 645 , 652, 659, 666, 673 , 680 687, 694, 701, 708, 715, 719 505, 512, 519, 526, 533, 540, 547, 554 , 561 519, 526 547, 554, 561 15 15 547, 554, 561 1 , 8 ,r 2 2 , 29, 36, 43 , 50,r 57, 64, 71, 78, 85,r 92, 99, 106, 113 1 2 0 , 127, 134, 141, 148, 155, 162, 169 , 176, 183, 190, 197 , 204 994 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 450 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F3 (CONTINUED FROM PREVIOUS PAGE) C93012 M 81 NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) AXILLARY REGION-LEFT WEEK 37 C93013 M INGUINAL REGION-RIGHT WEEK 61 86 APPEARANCE THIN BEHAVIOR ATAXIC <UNSPECIFIED> HYPOACTIVE <UNSPECIFIED> DISCHARGE NASAL RED IN COLOR EYES RED DISCHARGE EYE-RIGHT EYES DOSE: 2 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 211, 218, 225, 232, 239, 246 253C, 3 3 0C, 407C, 484C, 561C 260C, 337C, 414C, 491C, 267C, 344C, 421C, 498C, 274C, 351C, 428C, 505C, 281C, 358C, 435C, 512C, 288C, 365C, 442C, 519C, 295C, 372C, 44 9C, 526C, 302C, 379C, 456C, 533C, 3 0 9C, 386C, 463C, 54 0C, 316C, 393C, 470C, 54 7C, 323C 400C 477C 554C 421C, 428C, 435C, 442C, 44 9C, 456C, 463C, 470C, 477C 589, 596 547, 554, 561, 596 547, 554, 561, 596 547, 554 547, 554, 561, 568 575, 596 995 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 451 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F3 (CONTINUED FROM PREVIOUS PAGE) C93013 M 86 NORMAL NO REMARKABLE OBSERVATIONS M 76 APPEARANCE HEAD TILT LEFT HUNCHED BEHAVIOR HYPOACTIVE <UNSPECIFIED> EXCRETION FEW FECES NO FECES EYES RED DISCHARGE EYES NORMAL NO REMARKABLE OBSERVATIONS DOSE: 2 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 1 , 8 ,r 15, 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 582 528, 530 530 528, 530 528 530 519, 526, 528, 530 1 , 8 ,r 15, 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491 996 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 452 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F3 (CONTINUED FROM PREVIOUS PAGE) C93014 M 76 MASS(ES) AXILLARY REGION-LEFT WEEK 73 INGUINAL REGION-LEFT WEEK 72 C93015 M 102 APPEARANCE SWOLLEN VAGINA THIN BEHAVIOR HYPOACTIVE <UNSPECIFIED> DISCHARGE GENITAL RED IN COLOR EXCRETION NO FECES EYES RED DISCHARGE EYES SKIN & PELAGE RED HAIR COAT DORSAL-CERVICAL NORMAL NO REMARKABLE OBSERVATIONS DOSE: 2 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 505C, 512C, 519C, 526C 52 8C, 530C 498C, 505C, 512C, 519C 526C, 528C, 530C 666 680, 687 712 470 712 712 547,554 1, 8 , 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 997 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 453 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F3 (CONTINUED FROM PREVIOUS PAGE) C93015 M 102 NORMAL NO REMARKABLE OBSERVATIONS C93016 D MASS(ES) VENTRAL-ABDOMINAL WEEK 97 82 APPEARANCE HEAD TILT RIGHT MALOCCLUSION MISSING TEETH THIN BEHAVIOR ATAXIC <UNSPECIFIED> EXCRETION FEW FECES DOSE: 2 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 113, 204, 295, 386, 477, 582, 12 0 , 127, 2 1 1 , 218, 302, 309, 393, 400, 484, 491, 589, 596, 134, 225, 316, 407, 498, 603, 141, 232, 323, 414, 505, 610, 148, 239, 330, 421, 512, 617, 155, 246, 337, 428, 519, 624, 162, 253, 344, 435, 526, 631, 169, 260, 351, 442, 533, 638, 176, 267, 358, 449, 540, 645, 183, 274, 365, 456, 561, 652, 190, 281, 372, 463, 568, 659 197 288 379 470 575 673C, 680C, 687C, 694C, 701C, 708C, 712C 504- 505, 512, 519, 526, 533, 540, 547, 554, 561, 568, 571 64, 71, 78, 85 , 99,. 106,. 113,. 1 2 0 , 127,. 134, 141, 148,, 155, 162 169, 176 , 183, 2 1 1 ,. 218, 225,. 232, 239,. 246,, 253, 260, 267, 274 281, 288 , 295, 302, 309,. 316,. 323, 330, 337,, 344, 351,, 358, 365 372, 379 , 386, 393, 400, 407,. 414, 421,. 428, 435, 442, 449, 456 463, 470 , 477, 484, 491, 498, 504- 505, 512, 519, 526, 533, 540 547, 554 , 561, 568, 571 442, 449 , 456, 463,. 470, 477,. 484, 491, 498, 504- 505, 512, 519 526, 533 , 540, 547,. 554, 561,. 568, 571 491, 504 -505, 512, 519, 526, 533, 540, 547, 554, 561, 568, 571 504-505, 512, 519, 526, 533 491, 504-505, 512, 519 998 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 454 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F3 (CONTINUED FROM PREVIOUS PAGE) C93016 D 82 SKIN & PELAGE ROUGH HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS C93017 M 91 SKIN & PELAGE SORE/SCAB VENTRAL-ABDOMINAL-LEFT NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 33 C93018 2 14 NORMAL NO REMARKABLE OBSERVATIONS DOSE: 2 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 498, 504-505, 512, 519, 526, 533, 540, 547,. 554, 561, 568, 571 1 , 8 , 15, 22, 29, 36, 43 , 50,, 57, 92, 190, 197, 204 624, 631 1 , 8 , 15, 22, 29, 36, 43 , 50, 57, 64, 71 , 78, 85 , 92, 99, 106 113, 1 2 0 , 127, 134 , 141, 148, 155, 162, 169, 176 , 183, 190 , 197 204, 2 1 1 , 218 225C, 302C, 379C, 456C, 533C, 610C, 232C , 239C, 309C , 316C, 386C , 393C, 463C , 470C, 540C , 547C, 617C , 624C, 246C, 323C, 400C, 477C, 554C, 631C 253C, 330C, 407C, 484C, 561C, 260C , 267C , 274C, 337C , 344C , 351C, 414C , 421C , 428C, 491C , 498C , 505C, 568C , 575C , 582C, 281C, 358C, 435C, 512C, 58 9C, 288C , 295C 365C , 372C 442C , 449C 519C , 526C 596C , 603C 1, 8 , 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92-93 999 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 455 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F3 DOSE: 2 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C93019 M 45 APPEARANCE HUNCHED EXCRETION NO FECES SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE YELLOW HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS C93020 D MASS(ES) VENTRAL-ABDOMINAL WEEK 43 66 NORMAL NO REMARKABLE OBSERVATIONS 309 309 302, 309 309 1 , 8 , 15, 22, 29, 36, 43,, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127, 134, 141, 148, 155, 162 , 169, 176, 183,. 190, 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281, 288 295C, 302C 1 , 8 ,r 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407 , 414, 421, 428, 435 , 442, 449, 456, 461 1000 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 456 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F3 DOSE: 2 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C93021 M 63 APPEARANCE HEAD TILT RIGHT THIN SKIN & PELAGE RED HAIR COAT VENTRAL-ABDOMINAL ROUGH HAIR COAT YELLOW HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS C93022 T MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 51 103 SKIN & PELAGE RED HAIR COAT HEAD-CRANIAL NORMAL NO REMARKABLE OBSERVATIONS 400, 407, 414, 421, 428, 435 421, 428, 435 421, 428 421, 428, 435 435 1 22, 8,, 15, , 29, 36, 43 , 50, 57, 64, 71, 78, 85, 92, 99, 106 120113, , 127,. 134, 141, 148, 155, 162, 169, 176, 183,. 190, 197 211204, , 218,. 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309,. 316, 323, 330, 337, 344 351C, 358C , 365C, 372C, 379C, 386C , 393C 519, 526, 533,. 540 , 547, 554 1 22, 8,r 15, , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 120113, , 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 211204, , 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407 , 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498 , 505, 512, 561, 568 , 575, 582, 589, 596 1001 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 457 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F3 (CONTINUED FROM PREVIOUS PAGE) C93022 T 103 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 87 C93023 2 C93024 M 14 NORMAL NO REMARKABLE OBSERVATIONS 86 APPEARANCE HUNCHED THIN EXCRETION FEW FECES EYES RED DISCHARGE EYE-LEFT EYES RESPIRATION IRREGULAR SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE NORMAL NO REMARKABLE OBSERVATIONS DOSE: 2 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 603C, 610C, 617C, 624C, 631C, 638C, 645C, 652C , 659C, 666C, 673C 680C, 687C, 694C, 701C, 708C, 715C, 719C 1 , 8 , 15, 22, 29, 36, 43 , 50, 57, 64 , 71, 78, 85, 92-93 598 596, 598 598 561, 568 589, 596, 598 598 554 1 , 8 ,r 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 1002 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 458 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F3 (CONTINUED FROM PREVIOUS PAGE) C93024 M 86 NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 61 C93025 D 101 APPEARANCE MALOCCLUSION NORMAL NO REMARKABLE OBSERVATIONS C93026 M 103 APPEARANCE MALOCCLUSION THIN DOSE: 2 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 386, 393, 400, 407, 414 421C, 428C, 435C, 442C, 44 9C, 456C, 463C, 470C, 477C, 484C, 491C 498C, 505C, 512C, 519C, 526C, 533C, 54 0C, 54 7C, 554C, 561C, 568C 575C, 582C, 58 9C, 596C, 598C 71 1, 8 , 15, 120, 127, 2 1 1 , 218, 302, 309, 393, 400, 484, 491, 575, 582, 666, 673, 2 2 , 29, 36 , 43,r 50, 134, 141, 148, 155, 225,. 232, 239, 246, 316,. 323, 330, 337, 407,. 414, 421, 428, 498, 505, 512, 519, 589,. 596, 603, 610, 680, 687, 694, 701, 57, 64, 78, 85,r 92, 162, 169 , 176, 183, 253, 260 , 267, 274, 344, 351 , 358, 365, 435, 442 , 449, 456, 526, 533 , 540, 547, 617, 624 , 631, 638, 704 99, 106, 113 190, 197, 204 281, 288, 295 372, 379, 386 463, 470, 477 554, 561, 568 645, 652, 659 92, 99, 106, 113, 1 2 0 , 127, 134, 141, 148, 155, 162, 169, 176 183, 190 , 197 , 204 , 211 , 218 , 225 , 232 , 239 , 246 , 253 , 260 , 267 274, 281 , 288 , 295 , 302 , 309 , 316 , 323 , 330 , 337 , 344 , 351 , 358 365, 372 , 379 , 386 , 393 , 400 , 407 , 414 , 421 , 428 , 435 , 442 , 449 456, 463 , 470 , 477 , 484 , 491 , 498 , 505 , 512 , 519 , 526 , 533 , 540 547, 554 , 561 , 568 , 575 , 582 , 589 , 596 , 603 , 610 , 617 , 624 , 631 638, 645 , 652 , 659 , 666 , 673 , 680 , 687 , 694 , 701 , 708 , 715 715 1003 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 459 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F3 (CONTINUED FROM PREVIOUS PAGE) C93026 M 103 EYES CLEAR DISCHARGE EYES PALE EYES RED DISCHARGE EYES SKIN & PELAGE BLUE SKIN VENTRAL-THORACIC-LEFT SORE/SCAB VENTRAL-THORACIC-LEFT NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) AXILLARY REGION-LEFT WEEK 92 C93027 M 91 APPEARANCE THIN BEHAVIOR HYPOACTIVE <UNSPECIFIED> EXCRETION FEW FECES DOSE: 2 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 400, 687 715 407 701, 708, 715 715 1 , 8 , 15, 22 , 29, 36, 43 , 50, 57, 64, 71, 78, 85 638C, 645C, 652C, 659C, 666C, 673C, 680C, 687C, 694C, 701C, 708C 715C 631 631 617, 624 1004 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 460 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F3 (CONTINUED FROM PREVIOUS PAGE) C93027 M 91 EYES RED DISCHARGE EYES SKIN & PELAGE ROUGH HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS C93028 M MASS(ES) INGUINAL REGION-RIGHT WEEK 79 92 APPEARANCE THIN BEHAVIOR HYPOACTIVE <UNSPECIFIED> EXCRETION FEW FECES DOSE: 2 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 624, 631 631 1 , 8 ,r 15, 2 2 , 29, 36, 43,r 50, 113, 1 2 0 , 127, 134 , 141, 148, 204, 2 1 1 , 218, 225 , 232, 239, 295, 302, 309, 316 , 323, 330, 386, 393, 400, 407 , 414, 421, 477, 484, 491, 498 , 505, 512, 589, 596, 603, 610 57, 64, 71, 78, 85, 155, 162 , 169, 176, 246, 253 , 260, 267, 337, 344 , 351, 358, 428, 435 , 442, 449, 519, 526 , 533, 540, 92, 99, 106 183, 190 , 197 274, 281 , 288 365, 372 , 379 456, 463 , 470 568, 575 , 582 547C, 554C, 561C 638 638 633 1005 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 461 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F3 (CONTINUED FROM PREVIOUS PAGE) C93028 M 92 EYES RED DISCHARGE EYE-LEFT EYES NORMAL NO REMARKABLE OBSERVATIONS C93029 M MASS(ES) INGUINAL REGION-RIGHT WEEK 88 VENTRAL-ABDOMINAL-RIGHT WEEK 91 72 APPEARANCE HEAD TILT LEFT SWOLLEN PAW-HIND-RIGHT THIN BEHAVIOR HYPOACTIVE MODERATE SEVERE DOSE: 2 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 631, 633 638 1 , 8 ,r 15, 2 2 , 29, 36 , 43, 50, 113, 1 2 0 , 127,. 134, 141 , 148, 204, 2 1 1 , 218, 225, 232 , 239, 295, 302, 309,. 316, 323 , 330, 386, 393, 400, 407, 414 , 421, 477, 484, 491, 498, 505 , 512, 568, 575, 582, 589, 596 , 603 57, 64, 71, 78, 85, 155, 162 , 169, 176, 246, 253 , 260, 267, 337, 344 , 351, 358, 428, 435 , 442, 449, 519, 526 , 533, 540, 92, 99, 106 183, 190 , 197 274, 281 , 288 365, 372 , 379 456, 463 , 470 547, 554 , 561 610, 617C, 624C, 63 1C, 633C, 638C 631C, 633C, 638C 500 499 498-500 499 500 1006 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 462 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F3 (CONTINUED FROM PREVIOUS PAGE) C93029 M 72 EXCRETION FEW FECES NO FECES SKIN & PELAGE ROUGH HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) VENTRAL-CERVICAL WEEK 33 WEEK 53 M 96 BEHAVIOR HYPOACTIVE <UNSPECIFIED> EXCRETION FEW FECES SKIN & PELAGE SORE/SCAB PAW-HIND-LEFT NORMAL NO REMARKABLE OBSERVATIONS DOSE: 2 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 498-499 500 498-500 1 , 8 ,. 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85 , 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162, 169, 176 , 183,, 190, 197 204, 2 1 1 , 218, 225 , 232, 239, 246, 253, 260, 267 , 274, 281, 288 295, 302, 309, 316 , 323, 330, 337, 344, 351, 358 400C, 407C, 414C, 421C, 428C, 435C , 442C, 449C, 456C, 463C, 470C 477C,. 484C, 491C, 498-500C 365C, 372C, 379C, 386C, 393C 670 670 617, 624, 631, 638 , 645, 652, 659, 666, 670 1 , 8 ,. 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85 , 92, 99, 106 1007 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 463 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F3 (CONTINUED FROM PREVIOUS PAGE) C93030 M 96 NORMAL NO REMARKABLE OBSERVATIONS C93031 1 C93032 M 4 NORMAL NO REMARKABLE OBSERVATIONS 80 APPEARANCE HUNCHED THIN BEHAVIOR ATAXIC <UNSPECIFIED> HYPOACTIVE <UNSPECIFIED> DISCHARGE NASAL RED IN COLOR EXCRETION FEW FECES NORMAL NO REMARKABLE OBSERVATIONS DOSE: 2 PPM DAYS 1-736 OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 113, 204, 295, 386, 477, 568, 1 2 0 , 127 , 134, 141, 2 1 1 , 218 , 225,. 232, 302, 309 , 316,. 323, 393, 400 , 407,. 414, 484, 491 , 498, 505, 575, 582 , 589,. 596, 148 , 155, 239 , 246, 330 , 337, 421 , 428, 512 , 519, 603 , 610 162 , 169, 253 , 260, 344 , 351, 435 , 442, 526 , 533, 176, 267, 358, 449, 540, 183, 274, 365, 456, 547, 190, 281, 372, 463, 554, 197 288 379 470 561 1 , 8 ,r 15, 22 554, 556 547, 554, 556 547, 554, 556 547, 554, 556 547 526, 533, 540, 556 1 , 8 ,r 15, 2 2 , 29, 36, 43,, 50, 57, 64, 71, 78, 85, 92, 99, 10 6 113, 1 2 0 , 127,. 134, 141, 148, 155,. 162, 169, 176, 183,. 190, 197 204, 2 1 1 , 218,. 225, 232, 239, 246,. 253, 260, 267, 274, 281, 288 1008 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 464 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F3 (CONTINUED FROM PREVIOUS PAGE) C93032 M 80 NORMAL NO REMARKABLE OBSERVATIONS C93033 M WART LIKE LESION(S) VENTRAL-ABDOMINAL WEEK 74 66 APPEARANCE HEAD TILT RIGHT HUNCHED MALOCCLUSION THIN DISCHARGE CLEAR ORAL <UNSPECIFIED> MODERATE EXCRETION FEW FECES RESPIRATION AUDIBLE SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE DOSE: 2 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470 477, 484, 491, 498, 505 512C, 519C, 526C 365, 456 456 358 456 372, 379, 386, 393, 400, 407, 414, 421, 428, 435, 442, 449 414 456 288, 295 1009 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 465 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F3 (CONTINUED FROM PREVIOUS PAGE) C93033 M 66 SKIN & PELAGE SORE/SCAB DORSAL-CERVICAL NORMAL NO REMARKABLE OBSERVATIONS C93034 D MASS(ES) AXILLARY REGION-LEFT WEEK 45 AXILLARY REGION-RIGHT WEEK 45 INGUINAL REGION-LEFT WEEK 43 VENTRAL-ABDOMINAL-RIGHT WEEK 40 83 SKIN & PELAGE SORE/SCAB VENTRAL-ABDOMINAL-LEFT NORMAL NO REMARKABLE OBSERVATIONS DOSE: 2 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 379 1 , 8 , 15, 22, 29, 36, 43,, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127, 134, 141, 148, 155, 162 , 169, 176, 183,. 190, 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253 , 260, 267 309, 316, 323, 330, 337,. 344, 351, 358, 365, 372, 379, 386, 393 400, 407, 414, 421, 428, 435C,, 442C, 449C, 456C 309, 316, 323 295C, 302C , 309C, 316C, 323C 274, 281, 288, 295, 302, 309, 316, 323C, 330C, 337C, 344C, 351C 358C, 365C , 372C, 379C, 386C, 393C, 400C, 407C 526, 533, 540, 547, 554, 561, 568, 575 1 , 8 ,r 15, 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 323, 330, 337,. 344, 351, 358, 386, 393 , 400, 407, 414, 421 , 428 435 1010 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 466 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F3 (CONTINUED FROM PREVIOUS PAGE) C93034 D 83 MASS(ES) AXILLARY REGION-LEFT WEEK 72 AXILLARY REGION-RIGHT WEEK 73 INGUINAL REGION-LEFT WEEK 73 INGUINAL REGION-RIGHT WEEK 43 WEEK 73 VENTRAL-ABDOMINAL-LEFT WEEK 64 VENTRAL-ABDOMINAL-RIGHT WEEK 76 VENTRAL-CERVICAL WEEK 53 C93035 2 14 NORMAL NO REMARKABLE OBSERVATIONS C93036 D 85 APPEARANCE CONVULSIONS OBES TREMORS ENTIRE BODY BEHAVIOR RECUMBENT DORSAL DOSE: 2 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 498C, 505C , 512C, 519C, 526C , 533C , 540C , 547C , 554C, 561C , 568C 575C 505, 512, 519C, 526C, 533C, 54 0C, 54 7C, 554C, 561C, 568C, 575C 505C, 512C , 519C, 526C, 533C , 540C , 547C , 554C , 561C, 568C , 575C 295C, 302C , 309C, 316C 505, 512, 519, 526 , 533,, 540 , 547C , 554C , 561, 568, 575 442C, 449C , 456C, 463C, 470C , 477C , 484C , 491C , 498C, 505C , 512C 519C, 526C , 533C, 54 0C, 547C , 554C 526C, 533C , 540C, 54 7C, 554C 365C, 372C , 379C 1, 8 , 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92-93 592 526, 533, 540, 547, 554, 561, 568, 575, 582, 589, 592 592 592 1011 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 467 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F3 (CONTINUED FROM PREVIOUS PAGE) C93036 D 85 RESPIRATION AUDIBLE IRREGULAR NORMAL NO REMARKABLE OBSERVATIONS C93037 M MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 83 66 APPEARANCE HEAD TILT LEFT MALOCCLUSION BEHAVIOR CIRCLING LEFT HYPOACTIVE <UNSPECIFI] EXCRETION FEW FECES NO FECES DOSE: 2 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 592 592 1 , 8 ,r 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85 113, 1 2 0 , 127, 134 , 141, 148, 155, 162 , 169, 176 204, 2 1 1 , 218, 225 , 232, 239, 246, 253 , 260, 267 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358 386, 393, 400, 407 , 414, 421, 428, 435 , 442, 449 477, 484, 491, 498 , 505, 512, 519 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 575C, 582C, 589C, 592C 459 456, 459 459 459 456 459 1012 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 468 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F3 (CONTINUED FROM PREVIOUS PAGE) C93037 M 66 EYES RED DISCHARGE EYE-LEFT NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) AXILLARY REGION-LEFT WEEK 58 VENTRAL-CERVICAL-LEFT WEEK 64 M 71 SKIN & PELAGE SORE/SCAB HEAD-CRANIAL NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) AXILLARY REGION-RIGHT WEEK 53 DOSE: 2 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 449 1 , 8 , 15, 2 2 , 29, 36 , 43 , 50, 57, 64, 71 , 78, 85, 92, 99, 106 113, 1 2 0 , 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 435 400, 407, 414, 421, 428C 442, 449, 456, 459 29, 36, 43 , 50 , 57, 64, 71, 78 1 , 8 , 15, 2 2 , 85, 92 , 99 , 106, 113 , 1 2 0 , 127,, 134, 141 , 148, 155 162, 169, 176, 183, 190, 197, 204, 2 1 1 , 218, 225, 232, 239, 246 253, 260, 267, 274, 281, 288, 295, 302, 309, 316, 323, 330, 337 344, 351, 358 365, 372, 379C , 386C , 393C, 400C, 407C, 414C, 421C , 428C, 435C 442C, 44 9C, 456C, 463C, 470C, 477C, 484C, 491C 1013 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 469 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F3 (CONTINUED FROM PREVIOUS PAGE) C93038 M 71 WART LIKE LESION(S) DORSAL-CERVICAL WEEK 7 C93039 T 103 NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) AXILLARY REGION-RIGHT WEEK 75 INGUINAL REGION-LEFT WEEK 66 C93040 T 103 APPEARANCE THIN BEHAVIOR ATAXIC <UNSPECIFIED> SKIN & PELAGE ROUGH HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS DOSE: 2 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 43 1 22, 8,r 15, , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 120113, , 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 211204, , 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407 , 414, 421, 428, 435 , 442, 449 519, 526 456C, 533C, 610C, 687C, 463C, 54 0C, 617C, 694C, 470C, 54 7C, 624C, 701C, 477C, 554C, 631C, 708C, 484C, 561C, 638C, 715C, 491C, 568C, 645C, 719C 498C, 575C, 652C, 505C, 582C, 659C, 512C, 58 9C, 666C, 519C, 596C, 673C, 526C 603C 680C 715, 719 715, 719 701, 708, 715, 719 1 , 8 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 1014 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 470 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F3 (CONTINUED FROM PREVIOUS PAGE) C93040 T 103 NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) AXILLARY REGION-RIGHT WEEK 76 C93041 M 83 APPEARANCE HEAD TILT LEFT BEHAVIOR ATAXIC <UNSPECIFIED> HYPOACTIVE <UNSPECIFIED> EXCRETION FEW FECES SKIN & PELAGE RED HAIR COAT LATERA-RIGHT LATERAL-THORACIC-LEFT DOSE: 2 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 113, 204, 295, 386, 477, 120, 127, 2 1 1 , 218, 302, 309, 393, 400, 484, 491, 134 , 141,. 148, 225 , 232, 239, 316 , 323, 330, 407 , 414, 421, 498 , 505,. 512, 155, 246, 337, 428, 519 162, 253, 344, 435, 169, 260, 351, 442, 176, 267, 358, 449, 183,. 190, 274, 281, 365,. 372, 456,. 463, 197 288 379 470 526C,. 533C, 540C, 54 7C, 554C, 561C, 568C, 575C, 582C, 58 9C, 596C 603C, 610C, 617C, 624C, 631C, 638C, 645C, 652C, 659C, 666C, 673C 680C,. 687C, 694C, 701C, 708C, 715C, 719C 575 575 575 575 561, 568, 575 561, 568, 575 1015 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 471 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F3 (CONTINUED FROM PREVIOUS PAGE) C93041 M 83 NORMAL NO REMARKABLE OBSERVATIONS C93042 M MASS(ES) AXILLARY REGION-LEFT WEEK 73 AXILLARY REGION-RIGHT WEEK 76 WEEK 82 VENTRAL-ABDOMINAL WEEK 53 95 APPEARANCE SWOLLEN PAW-HIND-LEFT BEHAVIOR HYPOACTIVE <UNSPECIFIED> RESPIRATION AUDIBLE SKIN & PELAGE SORE/SCAB AXILLARY REGION-LEFT PAW-HIND- LEFT DOSE: 2 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 1 , 8 ,r 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 428, 435 , 442 449, 456, 463, 470 , 477, 484, 491, 498 505, 512, 519, 526, 533,, 540C,. 547C, 554C, 561C, 568C, 575C 526, 533, 540, 547 568, 575 365C, 372C , 379C, 386C, 393C, 400C, 407C, 414C, 421C 603, 610, 617, 624, 631, 638, 645, 652 665 665 610, 617 624, 631, 638, 645, 652, 659, 665 505, 512 575, 582, 589, 596, 603, 610, 617, 624, 631, 638, 645 652, 659 1016 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 472 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F3 (CONTINUED FROM PREVIOUS PAGE) C93042 M 95 SKIN & PELAGE SORE/SCAB PAW-HIND-RIGHT PAWS-HIND NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) AXILLARY REGION-LEFT WEEK 82 DORSAL WEEK 92 INGUINAL REGION-LEFT WEEK 79 INGUINAL REGION-RIGHT WEEK 79 VENTRAL-ABDOMINAL-LEFT WEEK 84 DOSE: 2 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 386, 393 519, 526, 533,. 540 , 547, 554, 561, 568 1, 8 113, 204, 295, 400, 491, 15, 2 2 , 29, 36, 43,r 50, 1 2 0, 127, 134 , 141, 148, 2 1 1 , 218, 225 , 232, 239, 302, 309, 316 , 323, 330, 407, 414, 421 , 428, 435, 498 57, 64, 71, 78, 85, 155, 162 , 169, 176, 246, 253 , 260, 267, 337, 344 , 351, 358, 442, 449 , 456, 463, 92, 99, 106 183, 190 , 197 274, 281 , 288 365, 372 , 379 470, 477 , 484 568, 575, 582C, 58 9C, 596C, 603C, 610C, 617C, 624C, 631C, 638C 645C 652C , 659C , 665C 638C 645C , 652C , 659C , 665C 547C 554C , 561C , 568C , 575C , 582C , 589C , 596C , 603C, 610C, 617C 624C 631C , 638C , 645C , 652C , 659C , 665C 547C 554C , 561C , 568C , 575C , 582C , 589C , 596C , 603C, 610C, 617C 624C 631C , 638C , 645C , 652C , 659C , 665C 582 1017 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 473 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F3 DOSE: 2 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C93043 M 97 SKIN & PELAGE SORE/SCAB AXILLARY REGION-LEFT AXILLARY REGION-RIGHT NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) AXILLARY REGION-LEFT WEEK 95 AXILLARY REGION-RIGHT WEEK 68 VENTRAL-ABDOMINAL WEEK 83 VENTRAL-CERVICAL-LEFT WEEK 73 VENTRAL-CERVICAL-RIGHT WEEK 84 659, 666, 673 519, 526, 533,. 540, 547 1 , 8 ,r 15, 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 659C, 666C 673C 470C, 477C 484C, 491C, 498C, 505C, 512C, 519C, 526C, 533C, 540C 54 7C, 554C 561C, 568C, 575C, 582C, 58 9C, 596C, 603C, 610C, 617C 624C, 631C 638C, 645C, 652C, 659C, 666C, 673C 575C, 582C 58 9C, 596C, 603C, 610C, 617C, 624C, 631C, 638C, 645C 652C, 659C 666C, 673C 505C, 512C 519C, 526C, 533C, 54 0C, 54 7C, 554C, 561C, 568C, 575C 582C, 589C 596C, 603C, 610C, 617C, 624C, 631C, 638C, 645C, 652C 659C, 666C 673C 582C, 589C 596C, 603C, 610C, 617C, 624C, 631C, 638C, 645C, 652C 659C, 666C 673C 1018 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 474 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F3 DOSE: 2 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C93044 M 66 APPEARANCE HEAD TILT LEFT MALOCCLUSION THIN BEHAVIOR HYPOACTIVE MODERATE EXCRETION FEW FECES SKIN & PELAGE SORE/SCAB HEAD-ENTIRE NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) AXILLARY REGION-RIGHT WEEK 56 442, 449, 456 456 449, 456 456 456 449, 456 1 , 8 ,r 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 428, 435 386C, 393C, 400C, 407C, 414C, 421C 1019 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 475 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F3 DOSE: 2 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C93045 1 C93046 M 4 NORMAL NO REMARKABLE OBSERVATIONS 80 APPEARANCE PROLAPSED VAGINA BEHAVIOR COLD TO TOUCH ENTIRE BODY DISCHARGE GENITAL RED IN COLOR SKIN & PELAGE SORE/SCAB VENTRAL-CERVICAL-LEFT NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) INGUINAL REGION-LEFT WEEK 73 INGUINAL REGION-RIGHT WEEK 58 1, 8 , 15, 22 554 554 554 498, 505, 512, 519, 526, 533, 540, 547, 554 1 , 8 , 15, 2 2 , 29, 36, 43, 50 , 57, 64, 71 , 78, 85 , 92, 99, 106 113, 1 2 0, 127, 134, 141, 148 , 155, 162, 169, 176 , 183, 190, 197 204, 2 1 1 , 218, 225, 232, 239 , 246, 253, 260, 267 , 274, 281, 288 295, 302, 309, 316, 323, 330 , 337, 344, 351, 358 , 365, 372, 379 386, 393 505, 512, 519C , 526C, 533C, 54 0C, 54 7C, 554C 400, 407C, 414C, 421C, 428C, 435C, 442C, 449C , 456C, 463C, 470C 477C, 484C', 491C, 498C, 505C , 512C , 519C , 526C, 533C, 540C, 547C 1020 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 476 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F3 (CONTINUED FROM PREVIOUS PAGE) C93046 M 80 MASS(ES) INGUINAL REGION-RIGHT WEEK 58 VENTRAL-CERVICAL-LEFT WEEK 64 C93047 T 103 APPEARANCE OBES SKIN & PELAGE BROWN SKIN VENTRAL-ABDOMINAL RED HAIR COAT DORSAL-CERVICAL NORMAL NO REMARKABLE OBSERVATIONS DOSE: 2 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 554C 442C, 44 9C, 456C, 463C, 470C, 477C, 484C, 491C, 498C, 505C, 512C 519C, 52 6C, 53 3C, 54 0C, 54 7C, 554C 505, 512, 519, 526, 533, 540, 547, 554, 561, 568, 575, 582, 589 596, 603, 610, 617, 624, 631, 638, 645, 652, 659, 666, 673, 680 687, 694, 701, 708, 715, 719 701 547 1 , 8 ,r 15, 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498 1021 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 477 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F3 DOSE: 2 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C93048 T 103 NORMAL NO REMARKABLE OBSERVATIONS C93049 M 70 APPEARANCE HEAD TILT LEFT BEHAVIOR ATAXIC MODERATE RECUMBENT LATERAL EXCRETION FEW FECES NO FECES EYES RED DISCHARGE EYES NORMAL NO REMARKABLE OBSERVATIONS 1, 8 15, 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582, 589, 596, 603, 610, 617 , 624, 631, 638, 645 , 652 659, 666, 673, 680, 687, 694, 701, 708 , 715, 719 484 484 484 477, 484 484 463, 470, 477, 484 1 , 8 ,r 15, 2 2 , 29, 36, 43,, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0, 127,. 134, 141, 148, 155,. 162, 169, 176, 183,. 190, 197 204, 2 1 1 , 218,. 225, 232, 239, 246,. 253, 260, 267, 274, 281, 288 1022 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 478 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F3 (CONTINUED FROM PREVIOUS PAGE) C93049 M 70 NORMAL NO REMARKABLE OBSERVATIONS C93050 M APPEARANCE THIN EXCRETION FEW FECES NONFORMED FECES EYES RED DISCHARGE EYES NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) INGUINAL REGION-RIGHT WEEK 79 VENTRAL-ABDOMINAL-RIGHT WEEK 76 DOSE: 2 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 295, 302 , 309, 316, 323, 330, 337, 344, 351, 358, 365, 372 , 379 386, 393 , 400, 407, 414, 421, 428, 435, 442, 449, 456 683 683 683 683 1 , 8 , 15 , 22, 29, 36, 43 , 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120 , 127, 134, 141, 148, 155, 162, 169, 176, 183, 190 , 197 204, 211 , 218, 225, 232, 239, 246, 253, 260, 267, 274, 281 , 288 295, 302 , 309, 316, 323, 330, 337, 344, 351, 358, 365, 372 , 379 386, 393 , 400, 407, 414, 421, 428, 435, 442, 449, 456, 463 , 470 477, 484 , 491, 498, 505, 512, 519 54 7C, 554C , 561C, 568C, 575C, 582C , 589C, 596C, 603C, 610C , 617C 624C, 63 1C , 638C, 645C, 652C, 659C , 666C, 673C, 680C, 683C 526C, 53 3C , 540C, 547C, 554C, 561C , 568C, 575C, 582C, 589C , 596C 603C, 61 0C , 617C, 624C, 631C, 638C , 645C, 652C, 659C, 666C , 673C 680C, 68 3C 1023 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 44 MASS(ES) 46 AXILLARY REGION-LEFT WEEK 44 47 MASS(ES) AXILLARY REGION-LEFT WEEK 44 48 MASS(ES) AXILLARY REGION-LEFT WEEK 44 78 MASS(ES) AXILLARY REGION-LEFT WEEK 78 79 MASS(ES) 81 AXILLARY REGION-LEFT WEEK 78 82 MASS(ES) AXILLARY REGION-LEFT WEEK 78 83 MASS(ES) 84 AXILLARY REGION-LEFT WEEK 78 76 MASS(ES) 80 DORSAL WEEK 76 20 X 15MM 15 X 15MM 10 X 10MM 20 X 20MM 30 X 30MM 30 X 30MM 40 X 40MM 15 X 15MM 1024 Covance 6329-183 3M T-6295 PAGE: 479 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 81 MASS(ES) 84 DORSAL WEEK 76 46 MASS(ES) 52 INGUINAL REGION-LEFT WEEK 46 53 MASS(ES) 56 INGUINAL REGION-LEFT WEEK 46 57 MASS(ES) INGUINAL REGION-LEFT WEEK 46 58 MASS(ES) 59 INGUINAL REGION-LEFT WEEK 46 60 MASS(ES) 61 INGUINAL REGION-LEFT WEEK 46 81 MASS(ES) 84 INGUINAL REGION-LEFT WEEK 81 76 MASS(ES) 78 INGUINAL REGION-RIGHT WEEK 76 20 X 20MM 15 X 10MM 30 X 30MM 30 X 50MM 50 X 35MM 25 X 20MM 10 X 10MM 15 X 15MM 1025 Covance 6329-183 3M T-6295 PAGE: 480 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 79 MASS(ES) 82 INGUINAL REGION-RIGHT WEEK 76 83 MASS(ES) 84 INGUINAL REGION-RIGHT WEEK 76 79 MASS(ES) 83 VENTRAL-CERVICAL WEEK 79 84 MASS(ES) 84 VENTRAL-CERVICAL WEEK 79 62 MASS(ES) 64 VENTRAL-THORACIC WEEK 62 65 MASS(ES) 68 VENTRAL-THORACIC WEEK 62 69 MASS(ES) 73 VENTRAL-THORACIC WEEK 62 74 MASS(ES) VENTRAL-THORACIC WEEK 62 30 X 20MM 40 X 40MM 15 X 15MM 10 X 10MM 10 X 10MM 15 X 15MM 30 X 20MM 40 X 30MM 1026 Covance 6329-183 3M T-6295 PAGE: 481 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 75 MASS(ES) VENTRAL-THORACIC WEEK 62 76 MASS(ES) 78 VENTRAL-THORACIC WEEK 62 79 MASS(ES) 80 VENTRAL-THORACIC WEEK 62 81 MASS(ES) 83 VENTRAL-THORACIC WEEK 62 84 MASS(ES) 84 VENTRAL-THORACIC WEEK 62 73 MASS(ES) 82 INGUINAL REGION-LEFT WEEK 73 74 MASS(ES) 83 VENTRAL-ABDOMINAL-RIGHT WEEK 74 76 MASS(ES) 82 VENTRAL-CERVICAL WEEK 76 40 X 20MM 40 X 30MM 30 X 20MM 50 X 25MM 30 X 30MM 10 X 10MM 30 X 30MM 10 X 10MM 1027 Covance 6329-183 3M T-6295 PAGE: 482 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT F3 400 58 TO 414 60 407 59 414 60 TO 421 61 428 62 435 63 TO 456 66 463 67 TO 477 69 484 70 TO 540 78 547 79 TO 554 80 EYES OTHER MASS(ES) VENTRAL-ABDOMINAL WEEK 59 MASS(ES) VENTRAL-ABDOMINAL WEEK 59 MASS(ES) VENTRAL-ABDOMINAL WEEK 59 MASS(ES) VENTRAL-ABDOMINAL WEEK 59 MASS(ES) VENTRAL-ABDOMINAL WEEK 59 MASS(ES) VENTRAL-ABDOMINAL WEEK 59 MASS(ES) VENTRAL-ABDOMINAL WEEK 59 SWOLLEN RIGHT EYELID 15 X 10MM 30 X 20MM 50 X 30MM 45 X 20MM 50 X 30MM 65 X 50MM 70 X 60MM 1028 Covance 6329-183 3M T-6295 PAGE: 483 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C92999 C93001 F3 561 81 TO 568 82 575 83 TO 603 87 610 TO 643 92 526 76 TO 568 82 547 79 TO 568 82 F3 568 82 TO 584 84 540 78 TO 554 80 561 81 TO 575 83 CO CO MASS(ES) VENTRAL-ABDOMINAL WEEK 59 MASS(ES) VENTRAL-ABDOMINAL WEEK 59 MASS(ES) VENTRAL-ABDOMINAL WEEK 59 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 76 MASS(ES) VENTRAL-CERVICAL WEEK 79 MASS(ES) INGUINAL REGION-LEFT WEEK 82 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 76 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 76 70 X 50MM 60 X 50MM 40 X 40MM 10 X 10MM 10 X 10MM 10 X 10MM 10 X 10MM 30 X 20MM 1029 Covance 6329-183 3M T-6295 PAGE: 484 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 84 MASS(ES) 84 VENTRAL-ABDOMINAL-LEFT WEEK 76 70 MASS(ES) 72 AXILLARY REGION-LEFT WEEK 70 73 MASS(ES) 74 AXILLARY REGION-LEFT WEEK 70 75 MASS(ES) 81 AXILLARY REGION-LEFT WEEK 70 48 MASS(ES) 49 INGUINAL REGION-LEFT WEEK 48 50 MASS(ES) INGUINAL REGION-LEFT WEEK 48 51 MASS(ES) 55 INGUINAL REGION-LEFT WEEK 48 56 MASS(ES) 57 INGUINAL REGION-RIGHT WEEK 56 30 X 30MM 10 X 10MM 15 X 15MM 20 X 20MM 20 X 20MM 20 X 30MM 30 X 30MM 30 X 40MM 1030 Covance 6329-183 3M T-6295 PAGE: 485 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 58 MASS(ES) 69 INGUINAL REGION-RIGHT WEEK 56 70 MASS(ES) 72 INGUINAL REGION-RIGHT WEEK 56 73 MASS(ES) 74 INGUINAL REGION-RIGHT WEEK 56 75 MASS(ES) 80 INGUINAL REGION-RIGHT WEEK 56 81 MASS(ES) 81 INGUINAL REGION-RIGHT WEEK 56 54 MASS(ES) 57 VENTRAL-ABDOMINAL WEEK 54 56 MASS(ES) 57 AXILLARY REGION-LEFT WEEK 56 58 MASS(ES) 64 AXILLARY REGION-LEFT WEEK 56 50 X 40MM 60 X 40MM 50 X 45MM 50 X 50MM 70 X 55MM 10 X 10MM 10 X 10MM 20 X 15MM 1031 Covance 6329-183 3M T-6295 PAGE: 486 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 65 MASS(ES) 72 AXILLARY REGION-LEFT WEEK 56 73 MASS(ES) AXILLARY REGION-LEFT WEEK 56 74 MASS(ES) AXILLARY REGION-LEFT WEEK 56 75 MASS(ES) AXILLARY REGION-LEFT WEEK 56 76 MASS(ES) 78 AXILLARY REGION-LEFT WEEK 56 79 MASS(ES) 85 AXILLARY REGION-LEFT WEEK 56 86 MASS(ES) AXILLARY REGION-LEFT WEEK 56 87 MASS(ES) 92 AXILLARY REGION-LEFT WEEK 56 30 X 30MM 35 X 40MM 40 X 40MM 40 X 50MM 50 X 55MM 55 X 70MM 70 X 70MM 80 X 70MM 1032 Covance 6329-183 3M T-6295 PAGE: 487 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C93003 C93005 F3 645 93 TO 659 95 MASS(ES) AXILLARY REGION-LEFT WEEK 56 666 96 MASS(ES) TO 694 100 AXILLARY REGION-LEFT WEEK 56 701 101 MASS(ES) TO 708 102 AXILLARY REGION-LEFT WEEK 56 715 103 MASS(ES) TO 719 103 AXILLARY REGION-LEFT WEEK 56 F3 400 58 TO 421 61 MASS(ES) AXILLARY REGION-LEFT WEEK 51 428 62 MASS(ES) AXILLARY REGION-LEFT WEEK 51 435 63 TO 456 66 MASS(ES) AXILLARY REGION-LEFT WEEK 51 463 67 TO 477 69 MASS(ES) AXILLARY REGION-LEFT WEEK 51 90 X 80MM 100 X 80MM 80 X 80MM 90 X 90MM 20 X 15MM 20 X 20MM 25 X 25M 30 X 30MM 1033 Covance 6329-183 3M T-6295 PAGE: 488 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 70 MASS(ES) 72 AXILLARY REGION-LEFT WEEK 51 73 MASS(ES) 90 AXILLARY REGION-LEFT WEEK 51 79 MASS(ES) 82 INGUINAL REGION-RIGHT WEEK 79 91 MASS(ES) HEAD-MAXILLARY-LEFT WEEK 91 92 MASS(ES) 92 HEAD-MAXILLARY-LEFT WEEK 91 81 MASS(ES) 82 VENTRAL-ABDOMINAL-RIGHT WEEK 79 83 MASS(ES) 87 VENTRAL-ABDOMINAL-RIGHT WEEK 79 91 MASS(ES) 92 VENTRAL-ABDOMINAL-RIGHT WEEK 79 30 X 40MM 40 X 45MM 10 X 10MM 20 X 20MM 25 X 20MM 15 X 15MM 10 X 10MM 10 X 10MM 1034 Covance 6329-183 3M T-6295 PAGE: 489 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 37 MASS(ES) 41 AXILLARY REGION-LEFT WEEK 37 42 MASS(ES) 43 AXILLARY REGION-LEFT WEEK 37 44 MASS(ES) 56 AXILLARY REGION-LEFT WEEK 37 57 MASS(ES) AXILLARY REGION-LEFT WEEK 37 58 MASS(ES) 61 AXILLARY REGION-LEFT WEEK 37 62 MASS(ES) AXILLARY REGION-LEFT WEEK 37 63 MASS(ES) 64 AXILLARY REGION-LEFT WEEK 37 65 MASS(ES) 69 AXILLARY REGION-LEFT WEEK 37 25 X 20 MM 30 X 30MM 40 X 40MM 50 X 50MM 60 X 60MM 90 X 70MM 60 X 70MM 80 X 70MM 1035 Covance 6329-183 3M T-6295 PAGE: 490 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 70 MASS(ES) AXILLARY REGION-LEFT WEEK 37 71 MASS(ES) AXILLARY REGION-LEFT WEEK 37 72 MASS(ES) AXILLARY REGION-LEFT WEEK 37 73 MASS(ES) 76 AXILLARY REGION-LEFT WEEK 37 77 MASS(ES) 81 AXILLARY REGION-LEFT WEEK 37 61 MASS(ES) INGUINAL REGION-RIGHT WEEK 61 62 MASS(ES) INGUINAL REGION-RIGHT WEEK 61 63 MASS(ES) 69 INGUINAL REGION-RIGHT WEEK 61 100 X 100MM 100 X 80MM 90 X 80MM 100 X 100MM 115 X 95MM 20 X 20MM 40 X 30MM 20 X 20MM 1036 Covance 6329-183 3M T-6295 PAGE: 491 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 73 MASS(ES) 76 AXILLARY REGION-LEFT WEEK 73 72 MASS(ES) 76 INGUINAL REGION-LEFT WEEK 72 97 MASS(ES) 98 VENTRAL-ABDOMINAL WEEK 97 99 MASS(ES) 10. VENTRAL-ABDOMINAL WEEK 97 33 MASS(ES) 57 VENTRAL-ABDOMINAL-LEFT WEEK 33 58 MASS(ES) 65 VENTRAL-ABDOMINAL-LEFT WEEK 33 66 MASS(ES) 69 VENTRAL-ABDOMINAL-LEFT WEEK 33 70 MASS(ES) 71 VENTRAL-ABDOMINAL-LEFT WEEK 33 10 X 10MM 10 X 10MM 25 X 15MM 40 X 30MM 10 X 10MM 15 X 15MM 15 X 25MM 35 X 30MM 1037 Covance 6329-183 3M T-6295 PAGE: 492 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 72 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 33 73 MASS(ES) 84 VENTRAL-ABDOMINAL-LEFT WEEK 33 85 MASS(ES) 86 VENTRAL-ABDOMINAL-LEFT WEEK 33 87 MASS(ES) 88 VENTRAL-ABDOMINAL-LEFT WEEK 33 89 MASS(ES) 91 VENTRAL-ABDOMINAL-LEFT WEEK 33 43 MASS(ES) 44 VENTRAL-ABDOMINAL WEEK 43 51 MASS(ES) 57 VENTRAL-ABDOMINAL-LEFT WEEK 51 87 MASS(ES) 94 VENTRAL-ABDOMINAL-RIGHT WEEK 87 30 X 30MM 30 X 40MM 40 X 50MM 50 X 60MM 60 X 60MM 15 X 15MM 10 X 10MM 25 X 20MM 1038 Covance 6329-183 3M T-6295 PAGE: 493 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C93022 C93024 F3 659 95 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 87 666 96 MASS(ES) TO 701 101 VENTRAL-ABDOMINAL-RIGHT WEEK 87 708 102 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 87 715 103 MASS(ES) TO 719 103 VENTRAL-ABDOMINAL-RIGHT WEEK 87 F3 421 61 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 61 428 62 TO 435 63 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 61 442 64 TO 456 66 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 61 463 67 TO 477 69 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 61 30 X 25MM 40 X 30MM 50 X 40MM 60 X 50MM 15 X 15MM 20 X 20MM 30 X 35MM 40 X 50MM 1039 Covance 6329-183 3M T-6295 PAGE: 494 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C93024 C93026 F3 484 70 TO 512 74 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 61 519 75 TO 598 86 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 61 F3 638 92 TO 687 99 MASS(ES) AXILLARY REGION-LEFT WEEK 92 694 100 MASS(ES) TO 701 101 AXILLARY REGION-LEFT WEEK 92 708 102 MASS(ES) TO 715 103 AXILLARY REGION-LEFT WEEK 92 F3 547 79 TO 561 81 MASS(ES) INGUINAL REGION-RIGHT WEEK 79 F3 617 89 TO 638 92 MASS(ES) INGUINAL REGION-RIGHT WEEK 88 631 91 TO 638 92 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 91 50 X 70MM 60 X 70MM 20 X 15MM 30 X 30MM 40 X 30MM 10 X 10MM 10 X10MM 15 X 15MM 1040 Covance 6329-183 3M T-6295 PAGE: 495 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 58 MASS(ES) 61 VENTRAL-CERVICAL WEEK 33 62 MASS(ES) 67 VENTRAL-CERVICAL WEEK 33 68 MASS(ES) 69 VENTRAL-CERVICAL WEEK 33 70 MASS(ES) 72 VENTRAL-CERVICAL WEEK 33 72 MASS(ES) 72 VENTRAL-CERVICAL WEEK 33 53 MASS(ES) VENTRAL-CERVICAL WEEK 53 54 MASS(ES) 57 VENTRAL-CERVICAL WEEK 53 74 WART LIKE LESION(S) 76 VENTRAL-ABDOMINAL WEEK 74 30 X 25MM 35 X 35MM 40 X 35MM 40 X 45MM 40 X 20MM 10 X 10MM 20 X 20MM GENITAL 1041 Covance 6329-183 3M T-6295 PAGE: 496 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 63 MASS(ES) 66 AXILLARY REGION-LEFT WEEK 45 43 MASS(ES) INGUINAL REGION-LEFT WEEK 43 44 MASS(ES) 47 INGUINAL REGION-LEFT WEEK 43 47 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 40 48 MASS(ES) 59 VENTRAL-ABDOMINAL-RIGHT WEEK 40 72 MASS(ES) 74 AXILLARY REGION-LEFT WEEK 72 75 MASS(ES) AXILLARY REGION-LEFT WEEK 72 76 MASS(ES) 79 AXILLARY REGION-LEFT WEEK 72 10 X 10MM 20 X 20MM 25 X 25MM 20 X 15MM 10 X 10MM 10 X 10MM 15 X 15MM 20 X 20MM 1042 Covance 6329-183 3M T-6295 PAGE: 497 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 80 MASS(ES) 83 AXILLARY REGION-LEFT WEEK 72 75 MASS(ES) 83 AXILLARY REGION-RIGHT WEEK 73 73 MASS(ES) 74 INGUINAL REGION-LEFT WEEK 73 75 MASS(ES) INGUINAL REGION-LEFT WEEK 73 76 MASS(ES) INGUINAL REGION-LEFT WEEK 73 77 MASS(ES) 78 INGUINAL REGION-LEFT WEEK 73 79 MASS(ES) INGUINAL REGION-LEFT WEEK 73 80 MASS(ES) 83 INGUINAL REGION-LEFT WEEK 73 15 X 15MM 10 X 10MM 20 X 20MM 50 X 20MM 60 X 35MM 60 X 50MM 80 X 65MM 80 X 70MM 1043 Covance 6329-183 3M T-6295 PAGE: 498 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 43 MASS(ES) 46 INGUINAL REGION-RIGHT WEEK 43 79 MASS(ES) 80 INGUINAL REGION-RIGHT WEEK 73 64 MASS(ES) 71 VENTRAL-ABDOMINAL-LEFT WEEK 64 72 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 64 73 MASS(ES) 76 VENTRAL-ABDOMINAL-LEFT WEEK 64 77 MASS(ES) 80 VENTRAL-ABDOMINAL-LEFT WEEK 64 76 MASS(ES) 80 VENTRAL-ABDOMINAL-RIGHT WEEK 76 53 MASS(ES) 55 VENTRAL-CERVICAL WEEK 53 15 X 10MM 10 X 10MM 10 X 10MM 15 X 15MM 20 X 20MM 25 X 25MM 15 X 15MM 10 X 10MM 1044 Covance 6329-183 3M T-6295 PAGE: 499 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 83 MASS(ES) 84 VENTRAL-ABDOMINAL-LEFT WEEK 83 85 MASS(ES) 85 VENTRAL-ABDOMINAL-LEFT WEEK 83 62 MASS(ES) AXILLARY REGION-LEFT WEEK 58 55 MASS(ES) 56 AXILLARY REGION-RIGHT WEEK 53 57 MASS(ES) AXILLARY REGION-RIGHT WEEK 53 58 MASS(ES) AXILLARY REGION-RIGHT WEEK 53 59 MASS(ES) AXILLARY REGION-RIGHT WEEK 53 60 MASS(ES) AXILLARY REGION-RIGHT WEEK 53 15 X 15MM 20 X 20MM 15 X 15MM 20 X 20MM 30 X 25MM 45 X 40MM 45 X 40MM 45 X 40MM 1045 Covance 6329-183 3M T-6295 PAGE: 500 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C93038 F3 421 61 428 62 435 63 442 64 449 65 456 66 463 67 470 68 MASS(ES) AXILLARY REGION-RIGHT WEEK 53 MASS(ES) AXILLARY REGION-RIGHT WEEK 53 MASS(ES) AXILLARY REGION-RIGHT WEEK 53 MASS(ES) AXILLARY REGION-RIGHT WEEK 53 MASS(ES) AXILLARY REGION-RIGHT WEEK 53 MASS(ES) AXILLARY REGION-RIGHT WEEK 53 MASS(ES) AXILLARY REGION-RIGHT WEEK 53 MASS(ES) AXILLARY REGION-RIGHT WEEK 53 45 X 40MM 70 X 60MM 70 X 60MM 70 X 60MM 70 X 60MM 90 X 70MM 90 X 70MM 90 X 80MM 1046 Covance 6329-183 3M T-6295 PAGE: 501 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 69 MASS(ES) AXILLARY REGION-RIGHT WEEK 53 70 MASS(ES) AXILLARY REGION-RIGHT WEEK 53 71 MASS(ES) AXILLARY REGION-RIGHT WEEK 53 71 MASS(ES) AXILLARY REGION-RIGHT WEEK 53 71 MASS(ES) AXILLARY REGION-RIGHT WEEK 53 66 MASS(ES) 70 INGUINAL REGION-LEFT WEEK 66 71 MASS(ES) 74 INGUINAL REGION-LEFT WEEK 66 75 MASS(ES) INGUINAL REGION-LEFT WEEK 66 90 X 80MM 115 X 115MM 115 X 115MM 115 X 115MM 115 X 115MM 20 X 15MM 30 X 20MM 30 X 30MM 1047 Covance 6329-183 3M T-6295 PAGE: 502 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C93039 C93040 F3 526 76 TO 533 77 MASS(ES) INGUINAL REGION-LEFT WEEK 66 540 78 TO 547 79 MASS(ES) INGUINAL REGION-LEFT WEEK 66 554 80 TO 596 86 MASS(ES) INGUINAL REGION-LEFT WEEK 66 603 87 TO 638 92 MASS(ES) INGUINAL REGION-LEFT WEEK 66 645 93 TO 659 95 MASS(ES) INGUINAL REGION-LEFT WEEK 66 666 96 MASS(ES) TO 708 102 INGUINAL REGION-LEFT WEEK 66 715 103 MASS(ES) TO 719 103 INGUINAL REGION-LEFT WEEK 66 F3 526 76 TO 540 78 MASS(ES) AXILLARY REGION-RIGHT WEEK 76 30 X 35MM 40 X 40MM 50 X 50MM 55 X 55MM 60 X 55MM 60 X 60MM 70 X 60MM 10 X 10MM 1048 Covance 6329-183 3M T-6295 PAGE: 503 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE- TEXT C93040 C93041 C93042 F3 547 79 TO 603 87 MASS(ES) AXILLARY REGION-RIGHT WEEK 76 610 TO 617 89 CO CO MASS(ES) AXILLARY REGION-RIGHT WEEK 76 624 90 TO 638 92 MASS(ES) AXILLARY REGION-RIGHT WEEK 76 645 93 TO 652 94 MASS(ES) AXILLARY REGION-RIGHT WEEK 76 659 95 MASS(ES) TO 719 103 AXILLARY REGION-RIGHT WEEK 76 F3 540 78 TO 575 83 MASS(ES) AXILLARY REGION-LEFT WEEK 73 365 53 TO 421 61 MASS(ES) VENTRAL-ABDOMINAL WEEK 53 F3 582 84 TO 603 87 MASS(ES) AXILLARY REGION-LEFT WEEK 82 15 X 10MM 15 X 15MM 10 X 10MM 15 X 10MM 20 X 15MM 10 X 10MM 15 X 10MM 10 X 10MM 1049 Covance 6329-183 3M T-6295 PAGE: 504 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 88 MASS(ES) 93 AXILLARY REGION-LEFT WEEK 82 94 MASS(ES) 95 AXILLARY REGION-LEFT WEEK 82 92 MASS(ES) 94 DORSAL WEEK 92 95 MASS(ES) 95 DORSAL WEEK 92 79 MASS(ES) 81 INGUINAL REGION-LEFT WEEK 79 82 MASS(ES) INGUINAL REGION-LEFT WEEK 79 83 MASS(ES) 84 INGUINAL REGION-LEFT WEEK 79 85 MASS(ES) 87 INGUINAL REGION-LEFT WEEK 79 15 X 15MM 10 X 10MM 10 X 10MM 30 X 30MM 10 X 10MM 20 X 20MM 30 X 30MM 40 X 30MM 1050 Covance 6329-183 3M T-6295 PAGE: 505 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 88 MASS(ES) 90 INGUINAL REGION-LEFT WEEK 79 91 MASS(ES) 95 INGUINAL REGION-LEFT WEEK 79 79 MASS(ES) 86 INGUINAL REGION-RIGHT WEEK 79 87 MASS(ES) 88 INGUINAL REGION-RIGHT WEEK 79 89 MASS(ES) 95 INGUINAL REGION-RIGHT WEEK 79 95 MASS(ES) 97 AXILLARY REGION-LEFT WEEK 95 68 MASS(ES) 69 AXILLARY REGION-RIGHT WEEK 68 70 MASS(ES) 72 AXILLARY REGION-RIGHT WEEK 68 40 X 40MM 70 X 50MM 10 X 10MM 15 X 15MM 10 X 10MM 15 X 15MM 20 X 15MM 30 X 30MM 1051 Covance 6329-183 3M T-6295 PAGE: 506 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 73 MASS(ES) 78 AXILLARY REGION-RIGHT WEEK 68 79 MASS(ES) 80 AXILLARY REGION-RIGHT WEEK 68 81 MASS(ES) 86 AXILLARY REGION-RIGHT WEEK 68 87 MASS(ES) 92 AXILLARY REGION-RIGHT WEEK 68 93 MASS(ES) 97 AXILLARY REGION-RIGHT WEEK 68 83 MASS(ES) 84 VENTRAL-ABDOMINAL WEEK 83 85 MASS(ES) 86 VENTRAL-ABDOMINAL WEEK 83 87 MASS(ES) 90 VENTRAL-ABDOMINAL WEEK 83 50 X 50MM 70 X 60MM 80 X 60MM 90 X 60MM 100 X 70MM 20 X 10MM 20 X 20MM 25 X 25MM 1052 Covance 6329-183 3M T-6295 PAGE: 507 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 91 MASS(ES) VENTRAL-ABDOMINAL WEEK 83 92 MASS(ES) 97 VENTRAL-ABDOMINAL WEEK 83 73 MASS(ES) 75 VENTRAL-CERVICAL-LEFT WEEK 73 76 MASS(ES) 77 VENTRAL-CERVICAL-LEFT WEEK 73 78 MASS(ES) 86 VENTRAL-CERVICAL-LEFT WEEK 73 87 MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 73 88 MASS(ES) 96 VENTRAL-CERVICAL-LEFT WEEK 73 97 MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 73 30 X 30MM 40 X 35MM 10 X 10MM 15 X 15MM 20 X 20MM 30 X 30MM 20 X 20MM 40 X 50MM 1053 Covance 6329-183 3M T-6295 PAGE: 508 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 97 MASS(ES) 97 VENTRAL-CERVICAL-LEFT WEEK 73 84 MASS(ES) VENTRAL-CERVICAL-RIGHT WEEK 84 85 MASS(ES) 90 VENTRAL-CERVICAL-RIGHT WEEK 84 91 MASS(ES) 93 VENTRAL-CERVICAL-RIGHT WEEK 84 94 MASS(ES) 97 VENTRAL-CERVICAL-RIGHT WEEK 84 56 MASS(ES) 61 AXILLARY REGION-RIGHT WEEK 56 75 MASS(ES) 80 INGUINAL REGION-LEFT WEEK 73 59 MASS(ES) INGUINAL REGION-RIGHT WEEK 58 20 X 20MM 20 X 20MM 30 X 30MM 50 X 45MM 50 X 55MM 10 X 10MM 10 X 10MM 10 X 10MM 1054 Covance 6329-183 3M T-6295 PAGE: 509 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 60 MASS(ES) 61 INGUINAL REGION-RIGHT WEEK 58 62 MASS(ES) INGUINAL REGION-RIGHT WEEK 58 63 MASS(ES) 69 INGUINAL REGION-RIGHT WEEK 58 70 MASS(ES) 75 INGUINAL REGION-RIGHT WEEK 58 76 MASS(ES) INGUINAL REGION-RIGHT WEEK 58 77 MASS(ES) 79 INGUINAL REGION-RIGHT WEEK 58 80 MASS(ES) 80 INGUINAL REGION-RIGHT WEEK 58 64 MASS(ES) 67 VENTRAL-CERVICAL-LEFT WEEK 64 20 X 20MM 50 X 50MM 40 X 35MM 60 X 70MM 70 X 70MM 80 X 80MM 90 X 90MM 10 X 10MM 1055 Covance 6329-183 3M T-6295 PAGE: 510 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 68 MASS(ES) 69 VENTRAL-CERVICAL-LEFT WEEK 64 70 MASS(ES) 71 VENTRAL-CERVICAL-LEFT WEEK 64 72 MASS(ES) 74 VENTRAL-CERVICAL-LEFT WEEK 64 75 MASS(ES) 80 VENTRAL-CERVICAL-LEFT WEEK 64 79 MASS(ES) 80 INGUINAL REGION-RIGHT WEEK 79 81 MASS(ES) 82 INGUINAL REGION-RIGHT WEEK 79 83 MASS(ES) 85 INGUINAL REGION-RIGHT WEEK 79 86 MASS(ES) INGUINAL REGION-RIGHT WEEK 79 20 X 20MM 30 X 30MM 20 X 20MM 30 X 25MM 20 X 15MM 40 X 40MM 30 X 30MM 45 X 35MM 1056 Covance 6329-183 3M T-6295 PAGE: 511 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 87 MASS(ES) 92 INGUINAL REGION-RIGHT WEEK 79 93 MASS(ES) 95 INGUINAL REGION-RIGHT WEEK 79 96 MASS(ES) 98 INGUINAL REGION-RIGHT WEEK 79 76 MASS(ES) 79 VENTRAL-ABDOMINAL-RIGHT WEEK 76 80 MASS(ES) 82 VENTRAL-ABDOMINAL-RIGHT WEEK 76 83 MASS(ES) 87 VENTRAL-ABDOMINAL-RIGHT WEEK 76 88 MASS(ES) 90 VENTRAL-ABDOMINAL-RIGHT WEEK 76 91 MASS(ES) 93 VENTRAL-ABDOMINAL-RIGHT WEEK 76 50 X 40MM 65 X 50MM 65 X 60MM 15 X 10MM 30 X 30MM 40 X 30MM 40 X 35MM 50 X 35MM 1057 Covance 6329-183 3M T-6295 PAGE: 512 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C93050 F3 652 94 TO 683 98 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 76 50 X 50MM Covance 6329-183 3M T-6295 PAGE: 513 1058 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 514 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F4 DOSE: 5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C93051 2 C93052 M 14 NORMAL NO REMARKABLE OBSERVATIONS 72 APPEARANCE HEAD TILT LEFT BEHAVIOR COLD TO TOUCH LIMBS-ALL EYES RED DISCHARGE EYE-LEFT NORMAL NO REMARKABLE OBSERVATIONS C93053 M 66 APPEARANCE HEAD TILT RIGHT MALOCCLUSION SKIN & PELAGE RED HAIR COAT LATERAL-CERVICAL-RIGHT YELLOW HAIR COAT 1 , 8 , 15, 2 2 , 29, 36, 43, 50, 57, 64, 71, 78, 85, 92-93 489, 491, 498, 500 500 500 1 , 8 ,r 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407 , 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484 459 449, 456, 459 449 421, 428, 435, 442, 449 1059 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 515 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F4 (CONTINUED FROM PREVIOUS PAGE) C93053 M 66 NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 42 C93054 M 72 APPEARANCE HEAD TILT RIGHT THIN BEHAVIOR ATAXIC <UNSPECIFIED> EXCRETION FEW FECES EYES RED DISCHARGE EYES NORMAL NO REMARKABLE OBSERVATIONS DOSE: 5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 1 , 8 , 15, 22, 29, 36, 43,, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127, 134, 141, 148, 155, 162 , 169, 176, 183,. 190, 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 288C, 295C, 302C, 309C, 316C, 323C, 330C, 337C, 344C, 351C, 358C 365C, 372C, 379C, 386C, 393C, 400C, 407C, 414C, 421C, 428C, 435C 442C, 44 9C, 456C, 459C 504 504 504 504 504 1 , 8 ,r 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 1060 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 516 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F4 (CONTINUED FROM PREVIOUS PAGE) C93054 M 72 NORMAL NO REMARKABLE OBSERVATIONS C93055 M MASS(ES) INGUINAL REGION-LEFT WEEK 71 77 BEHAVIOR HYPOACTIVE <UNSPECIFIED> EXCRETION FEW FECES SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE NORMAL NO REMARKABLE OBSERVATIONS DOSE: 5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470 477, 484 491C, 498C, 504C 533 532-533 1 , 8 ,r 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400 407 , 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491 498 , 505, 512, 519, 526 1061 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 517 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F4 DOSE: 5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C93056 M 99 APPEARANCE THIN EXCRETION FEW FECES NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) AXILLARY REGION-RIGHT WEEK 93 INGUINAL REGION-RIGHT WEEK 95 VENTRAL-ABDOMINAL WEEK 99 M 99 APPEARANCE THIN EXCRETION FEW FECES SKIN & PELAGE SORE/SCAB VENTRAL-ABDOMINAL 687, 691 691 1 , 8 ,r 15, 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582, 589, 596, 603, 610, 617 , 624, 631, 638 645, 652 659C, 666C, 673C, 680C, 687C, 691C 687C, 691C 691 554 1062 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 518 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F4 (CONTINUED FROM PREVIOUS PAGE) C93057 M 99 NORMAL NO REMARKABLE OBSERVATIONS C93058 1 C93059 T MASS(ES) AXILLARY REGION-RIGHT WEEK 68 VENTRAL-ABDOMINAL WEEK 77 WEEK 97 4 NORMAL NO REMARKABLE OBSERVATIONS NORMAL NO REMARKABLE OBSERVATIONS DOSE: 5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 1 , 8 ,. 15, 2 2 , 29, 36, 43 , 50, 57, 64, 71, 78, 85 , 92, 99, 106 113, 1 2 0 , 127,. 134, 141, 148, 155, 162, 169, 176 , 183, 190 , 197 204, 2 1 1 , 218,. 225, 232, 239, 246, 253, 260, 267 , 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344, 351, 358 , 365, 372 , 379 386, 393, 400,. 407, 414, 421, 428, 435, 442, 449 , 456, 463 470C, 477C', 484C, 491C, 498C, 505C , 512C, 519C, 526C, 533C , 540C 54 7C, 554C , 561C, 568C, 575C, 582C , 589C, 596C, 603C, 610C , 617C 624C, 631C , 638C, 645C, 652C, 659C , 666C, 673C, 680C, 687C , 691C 533, 54 0C, 547C 673C, 680C , 687C, 691C 1 , 8 ,. 15, 22 1 , 8 ,. 15, 2 2 , 29, 36, 43 , 50, 57, 64, 71, 78, 85 , 92, 99, 106 113, 1 2 0 , 127,. 134, 141, 148, 155, 162, 169, 176 , 183, 190 , 197 204, 2 1 1 , 218,. 225, 232, 239, 246, 253, 260, 267 , 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344, 351, 358 , 365, 372 , 379 386, 393, 400,. 407, 414, 421, 428, 435, 442, 449 , 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526, 533, 540 , 547, 554 , 561 568, 575, 582,. 589, 596, 603, 610, 617, 624, 631 , 638, 645 , 652 659, 666, 673, 680, 687, 694, 701, 708, 715, 722 , 729, 732 1063 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 519 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F4 DOSE: 5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C93060 T 105 APPEARANCE MALOCCLUSION C93061 D EYES CLEAR DISCHARGE EYE-LEFT NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) AXILLARY REGION-LEFT WEEK 93 80 APPEARANCE HUNCHED THIN EXCRETION FEW FECES SKIN & PELAGE PALE ENTIRE BODY 127, 218, 309, 400, 491, 582, 673, 134, 225, 316, 407, 498, 589, 680, 141, 232, 323, 414, 505, 596, 687, 148, 239, 330, 421, 512, 603, 694, 155, 246, 337, 428, 519, 610, 701, 162, 253, 344, 435, 526, 617, 708, 169, 260, 351, 442, 533, 624, 715, 176, 267, 358, 449, 540, 631, 722, 183, 274, 365, 456, 547, 638, 729, 190, 281, 372, 463, 554, 645, 733 197, 288, 379, 470, 561, 652, 204, 295, 386, 477, 568, 659, 211 302 393 484 575 666 701 1 , 8 , 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92 113, 120 645, 652, 659, 666, 673, 680, 687, 694, 701, 708, 715, 722, 729 733 554, 558 554, 558 533, 540, 554, 558 533, 540, 547, 554, 558 1064 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 520 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F4 (CONTINUED FROM PREVIOUS PAGE) C93061 D 80 NORMAL NO REMARKABLE OBSERVATIONS C93062 T MASS(ES) AXILLARY REGION-LEFT WEEK 73 105 APPEARANCE MALOCCLUSION NORMAL NO REMARKABLE OBSERVATIONS C93063 T 105 EYES RED DISCHARGE EYE-RIGHT DOSE: 5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 1, 8 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407 , 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498 , 519, 526 505, 512 477, 484 491, 498, 505, 512, 519, 526, 533, 540, 547, 554, 561 568, 575 582, 589, 596, 603, 610, 617, 624, 631, 638, 645, 652 659, 666 673, 680, 687, 694, 701, 708, 715, 722, 729, 731 1, 8 , 15 113, 120 204, 211 295, 302 386, 393 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 400, 407 , 414, 421, 428, 435 , 442, 449, 456, 463 , 470 246, 253 344, 351 435, 442 526, 533 652, 659 260, 267, 358, 365, 449, 456, 540, 547, 666, 673, 274, 372, 463, 554, 680, 281, 379, 470, 561, 687, 295, 386, 477, 568, 694, 302, 393, 484, 575, 701, 309, 400, 491, 582, 708, 316, 407, 498, 589, 715, 323, 414, 505, 596, 722, 330, 421, 512, 603, 729, 337 428 519 610 731 1065 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 521 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F4 (CONTINUED FROM PREVIOUS PAGE) C93063 T 105 SKIN & PELAGE SORE/SCAB AXILLARY REGION-RIGHT NORMAL NO REMARKABLE OBSERVATIONS C93064 2 C93065 M MASS(ES) AXILLARY REGION-RIGHT WEEK 100 INGUINAL REGION-RIGHT WEEK 101 VENTRAL-CERVICAL WEEK 102 14 NORMAL NO REMARKABLE OBSERVATIONS 93 APPEARANCE HUNCHED THIN BEHAVIOR HYPOACTIVE <UNSPECIFIED> EXCRETION FEW FECES DOSE: 5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 729, 731 1, 8,, 15, 22, 29, 36, 43,, 50, 57, 64, 71, 78, 85, 92, 99, 106 120113, , 127, 134, 141, 148, 155, 162 , 169, 176, 183,. 190, 197 211204, , 218, 225, 232, 239, 288, 617 , 624, 631, 638, 645 694C, 701C, 708C, 715C, 722C, 729C, 731C 701, 708, 715, 722, 729, 731 708C, 715C, 722C, 729C, 731C 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92-93 547, 554, 561, 568, 575, 582, 589, 596, 603, 610, 617, 624 554, 561, 568, 575, 582, 589, 596, 603, 610 533, 540, 547, 554, 561, 568 532-533 1066 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 522 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F4 (CONTINUED FROM PREVIOUS PAGE) C93065 M 93 EYES RED DISCHARGE EYE-LEFT EYES RESPIRATION AUDIBLE SKIN & PELAGE RED HAIR COAT VENTRAL-ABDOMINAL NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) AXILLARY REGION-LEFT WEEK 87 AXILLARY REGION-RIGHT WEEK 83 VENTRAL-ABDOMINAL-RIGHT WEEK 86 VENTRAL-CERVICAL WEEK 72 DOSE: 5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 540, 547, 554 532-533 617, 645 554, 561, 568,. 575, 582, 589, 596, 603 , 610, 617, 624 1 , 8 ,r 15, 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491 603, 610, 617, 624, 631, 638, 645 575, 582C, 589C, 596C, 603C, 610C, 617C, 624C, 631C, 638C, 645C 596, 603, 610, 617, 624, 631, 638C , 645C 498C, 505C, 512C, 519C, 526C, 532- 533C, 540C, 54 7C, 554C, 561C 568C 1067 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 523 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F4 DOSE: 5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C93066 M 93 SKIN & PELAGE SORE/SCAB VENTRAL-ABDOMINAL-RIGHT NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) AXILLARY REGION-LEFT WEEK 84 INGUINAL REGION-LEFT WEEK 70 VENTRAL-ABDOMINAL-LEFT WEEK 54 VENTRAL-ABDOMINAL-RIGHT WEEK 70 596, 603, 610, 617, 624, 631, 638, 645 1 , 8 , 15, 22, 29, 36, 43 , 50, 57, 64, 71 , 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281 , 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365 582, 589, 596, 603, 610C , 617C , 624C, 631C, 638C, 645C 484C, 491C, 498C, 505C, 512C, 519C , 526C , 533C, 540C, 547C , 554C 561C, 568C, 575C, 582C, 58 9C, 596C , 603C 372C, 379C, 386C, 393C, 400C, 407C , 414C , 421C, 428C, 435C , 442C 44 9C, 456C, 463C, 470C, 477C, 484C , 491C , 498C, 505C, 512C , 519C 526C, 533C, 540C, 547C, 554C, 561C , 568C , 575C, 582C, 589C , 596C 603C, 610C, 617C, 624C, 631C, 638C , 645C 484C, 491C, 498C, 505C, 512C, 519C , 526C , 533C, 540C, 547C , 554C 561C, 568C, 575C, 582C, 58 9C, 596C , 603C , 610C, 617C, 624C , 631C 638C, 645C 1068 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 524 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F4 DOSE: 5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C93067 1 C93068 D 4 NORMAL NO REMARKABLE OBSERVATIONS 103 APPEARANCE HEAD TILT RIGHT MALOCCLUSION THIN SKIN & PELAGE ALOPECIA NORMAL NO REMARKABLE OBSERVATIONS C93069 M 101 SKIN & PELAGE ALOPECIA NORMAL NO REMARKABLE OBSERVATIONS 1, 8 , 15, 22 715, 183, 274, 372, 589, 680, 715, 718 190, 281, 379, 596, 687, 718 197, 288, 386, 603, 694, 204, 295, 519, 610, 701, 2 1 1 , 218, 302, 316, 526, 533, 617, 624, 708, 715, 225, 323, 540, 631, 718 232, 330, 547, 638, 239, 337, 554, 645, 246, 344, 561, 652, 253, 351, 568, 659, 260 358 575 666 267 365 582 673 547, 554, 561, 568 , 575, 582, 589, 596 , 603, 610, 617, 624, 631 638, 645, 652, 659 , 666, 673, 680, 687 , 694, 701, 708, 715, 718 1 , 8 ,r 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 10 6 113, 1 2 0, 127, 134 , 141, 148, 155, 162 , 169, 176, 309, 393, 400 407, 414, 421, 428 , 435, 442, 449, 456 , 463, 470, 477, 484, 491 498, 505, 512 470, 568, 575, 617, 624, 673, 680 1 , 8 ,r 15, 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 10 6 113, 1 2 0, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190, 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281, 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463, 477 1069 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 525 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F4 (CONTINUED FROM PREVIOUS PAGE) C93069 M 101 NORMAL NO REMARKABLE OBSERVATIONS C93070 1 C93071 2 C93072 T MASS(ES) AXILLARY REGION-LEFT WEEK 87 AXILLARY REGION-RIGHT WEEK 86 INGUINAL REGION-LEFT WEEK 93 VENTRAL-THORACIC-RIGHT WEEK 92 4 NORMAL NO REMARKABLE OBSERVATIONS 14 NORMAL NO REMARKABLE OBSERVATIONS 105 NORMAL NO REMARKABLE OBSERVATIONS DOSE: 5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 484, 491, 498, 505, 512, 519, 526, 533, 540, 547, 554, 561, 582 589 603C, 610C, 617C, 624C, 631C , 638C, 645C, 652C, 659C , 666C, 673C 680C, 687C, 694C, 701-7 02C 596C, 603C, 610C, 617C, 624C , 631C, 638C, 645C, 652C , 659C, 666C 673C, 680C, 687C, 694C, 701- 702C 645C, 652C, 659C, 666C, 673C , 680C, 687C, 694C, 701- 702C 638C, 645C, 652C, 659C, 666C , 673C, 680C, 687C, 694C , 701-702C 1, 8 , 15, 22 1 , 8 ,r 15, 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92- 93 1 , 8 ,r 15, 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0, 127,. 134, 141, 148, 155, 162 , 169, 176, 183 , 190, 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253 , 260, 267, 274 , 281, 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365 , 372, 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456 , 463, 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547 , 554, 561 568, 575, 582, 589, 596 1070 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 526 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F4 (CONTINUED FROM PREVIOUS PAGE) C93072 T 105 MASS(ES) AXILLARY REGION-RIGHT WEEK 87 INGUINAL REGION-LEFT WEEK 103 C93073 D 75 APPEARANCE HEAD TILT RIGHT HUNCHED THIN BEHAVIOR ATAXIC <UNSPECIFIED> HYPOACTIVE <UNSPECIFIED> EXCRETION FEW FECES RESPIRATION AUDIBLE NORMAL NO REMARKABLE OBSERVATIONS DOSE: 5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 603C, 610C, 617C, 624C, 631C, 638C, 645C, 652C, 659C, 666C, 673C 680C, 687C, 694C, 701C, 708C, 715C, 722C, 729C, 732C 715C, 722C, 729C, 732C 505, 512, 519, 522 519, 522 498, 505, 512, 519, 522 505, 512 519, 522 491, 498, 505, 512 519, 522 1 , 8 ,r 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407 , 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484 1071 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 527 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F4 DOSE: 5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C93074 M 83 APPEARANCE HEAD TILT RIGHT BEHAVIOR ATAXIC <UNSPECIFIED> HYPOACTIVE <UNSPECIFIED> EXCRETION FEW FECES EYES RED DISCHARGE EYE-RIGHT NORMAL NO REMARKABLE OBSERVATIONS 561, 568, 575 575 575 316, 575 568 1, 8 , 15, 113, 120, 204, 211, 295, 302, 393, 400, 484, 491, 2 2 , 29, 36, 43,r 50, 127, 134 , 141, 148, 218, 225 , 232, 239, 309, 323 , 330, 337, 407, 414 , 421, 428, 498, 505 , 512, 519, 57, 64, 71, 78, 85, 155, 162 , 169, 176, 246, 253 , 260, 267, 344, 351 , 358, 365, 435, 442 , 449, 456, 526, 533 , 540, 547, 92, 99, 106 183, 190 , 197 274, 281 , 288 372, 379 , 386 463, 470 , 477 554 1072 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 528 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F4 DOSE: 5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C93075 1 C93076 M 4 NORMAL NO REMARKABLE OBSERVATIONS 94 APPEARANCE MALOCCLUSION MISSING TEETH THIN BEHAVIOR HYPOACTIVE <UNSPECIFIED> NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) AXILLARY REGION-RIGHT WEEK 84 LIMB-HIND-LEFT WEEK 59 1, 8 , 15, 22 43, 50, 57, 64,r 71, 78, 85, 92, 99, 106 , 113,r 1 2 0, 127 , 134, 141 148, 155 , 162, 169, 176, 183 , 190, 197, 204, 2 1 1 , 218, 225, 232 239, 246 , 253, 260, 267, 274 , 281, 288, 295, 302, 309, 316, 323 330, 337 , 344, 351, 358, 365 , 372, 379, 386, 393, 400, 407, 414 421, 428 , 435, 442, 449, 456 , 463, 470, 477, 484, 491, 498, 505 512, 519 , 526, 533, 540, 547 , 554, 561, 568, 575, 582, 589, 596 603, 610 , 617, 624, 631, 638 , 645, 652- 653 309, 316 , 323, 400, 407 , 414, 491, 498 , 505, 582, 589 , 596, 652- 653 330, 421, 512, 603, 337, 428, 519, 610, 344 , 351, 435 , 442, 526 , 533, 617 , 624, 358, 449, 540, 631, 365, 456, 547, 638, 372, 463, 554, 645, 379, 386, 470, 477, 561, 568, 652- 653 393 484 575 645, 652-653 1, 8 , 15, 22, 29, 36 582C, 589C, 596C, 603C, 610C, 617C, 624C, 631C, 638C, 645C 652-653C 407, 414C, 421C, 428C, 435C, 442C, 44 9C, 456C, 463C, 470C, 477C 484C,. 491C, 498C, 505C, 512C, 519C, 526C,. 533C, 540C, 547C, 554C 1073 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 529 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F4 (CONTINUED FROM PREVIOUS PAGE) C93076 M 94 MASS(ES) LIMB-HIND-LEFT WEEK 59 C93077 T 105 APPEARANCE SWOLLEN TAIL-PROXIMAL SKIN & PELAGE SORE/SCAB TAIL-PROXIMAL NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) AXILLARY REGION-LEFT WEEK 101 TAIL-PROXIMAL WEEK 97 DOSE: 5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 561C, 56 8C, 575C, 582C, 589C, 596C, 603C, 610C, 617C, 624C, 631C 638C, 64 5C, 652-653C 631, 638, 645, 652, 659, 666 708, 715, 722, 729, 733 1 , 8 ,r 15, 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582, 589, 596, 603, 610, 617 , 624 701C, 708C, 715C, 722C, 729C, 733C 673C, 680C, 687C, 694C, 701C, 708C, 715C, 722C, 729C, 733C 1074 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 530 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F4 DOSE: 5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C93078 T 105 NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) AXILLARY REGION-LEFT WEEK 104 VENTRAL-ABDOMINAL WEEK 98 VENTRAL-ABDOMINAL-LEFT WEEK 94 VENTRAL-ABDOMINAL-RIGHT WEEK 89 C93079 T 105 NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) AXILLARY REGION-LEFT WEEK 101 1 , 8 ,r 15, 2 2 , 29, 36 , 43,r 50, 113, 1 2 0, 127,. 134, 141, 148, 204, 2 1 1 , 218, 225, 232, 239, 295, 302, 309,. 316, 323, 330, 386, 393, 400, 407, 414, 421, 477, 484, 491, 498, 505, 512, 568, 575, 582, 589, 596, 603, 57, 64, 71, 78, 85, 155, 162 , 169, 176, 246, 253 , 260, 267, 337, 344 , 351, 358, 428, 435 , 442, 449, 519, 526 , 533, 540, 610 92, 99, 106 183, 190 , 197 274, 281 , 288 365, 372 , 379 456, 463 , 470 547, 554 , 561 722, 729, 731 680C, 687C , 694C, 701C 652C, 659C , 666C, 673C, 680C, 687C, 694C, 701C, 708C, 715C, 722C 72 9C, 731C 617C, 624C , 631C, 638C, 645C, 652C, 659C, 666C, 673C, 680C, 687C 694C, 701C , 708C, 715C, 722C, 729C, 731C 1 , 8 ,r 15, 2 2 , 29, 36, 43,r 50, 113, 1 2 0, 127, 134 , 141, 148, 204, 2 1 1 , 218, 225 , 232, 239, 295, 302, 309, 316 , 323, 330, 386, 393, 400, 407 , 414, 421, 57, 64, 71, 78, 85, 155, 162 , 169, 176, 246, 253 , 260, 267, 337, 344 , 351, 358, 428, 435 , 442, 449, 92, 99, 106 183, 190 , 197 274, 281 , 288 365, 372 , 379 456, 463 , 470 701C, 708C, 715C, 722C, 729C, 732C 1075 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 531 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F4 (CONTINUED FROM PREVIOUS PAGE) C93079 T 105 MASS(ES) AXILLARY REGION-RIGHT WEEK 69 C93080 T 105 NORMAL NO REMARKABLE OBSERVATIONS C93081 T 105 APPEARANCE MALOCCLUSION NORMAL NO REMARKABLE OBSERVATIONS DOSE: 5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 477C, 484C, 491C, 498C, 505C, 512C, 519C, 526C, 533C, 54 0C, 547C 554C, 561C, 568C, 575C, 582C, 58 9C, 596C, 603C, 610C, 617C, 624C 631C, 638C, 645C, 652C, 659C, 666C, 673C, 680C, 687C, 694C, 701C 708C, 715C, 722C, 729C, 732C 1, 8 15, 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582, 589, 596, 603, 610, 617 , 624, 631, 638, 645 , 652 659, 666, 673, 680, 687, 694, 701, 708 , 715, 722, 729, 733 624, 631, 729, 732 1 , 8 ,r 15, 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582, 589, 596, 603, 610, 617 , 638, 645, 652, 659 , 666 673, 680, 687,. 694, 701, 708, 715, 722 1076 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 532 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F4 DOSE: 5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C93082 D 97 EXCRETION FEW FECES EYES PALE EYES SKIN & PELAGE YELLOW HAIR COAT YELLOW SKIN PAWS- ALL TAIL NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) AXILLARY REGION-RIGHT WEEK 68 LIMB-FRONT-LEFT WEEK 45 673, 677 652, 659, 666, 673, 677 673, 677 673, 677 673, 677 1 , 8 ,r 15, 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 414, 421, 428, 435, 442, 449 , 456, 463, 484, 491 , 498 505, 512, 519,. 526, 533, 540, 547, 554 , 561, 568, 575, 582 , 589 596, 603, 610, 617, 624, 631, 638, 645 470, 477 309, 316, 323, 330, 337, 344, 351, 358 , 365, 372, 379, 386 , 393 400, 407 1077 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 533 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F4 DOSE: 5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C93083 M 105 APPEARANCE THIN NORMAL NO REMARKABLE OBSERVATIONS C93084 1 C93085 2 MASS(ES) VENTRAL-ABDOMINAL WEEK 95 VENTRAL-CERVICAL-LEFT WEEK 87 4 NORMAL NO REMARKABLE OBSERVATIONS 14 NORMAL NO REMARKABLE OBSERVATIONS 729 1 , 8 ,. 15, 22, 29, 36, 43 , 50, 57, 64, 71, 78, 85 , 92, 99, 106 113, 1 2 0, 127, 134, 141, 148, 155, 162, 169, 176 , 183 , 190 , 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253, 260, 267 , 274 , 281 , 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358 , 365 , 372 , 379 386, 393, 400, 407, 414, 421, 428, 435, 442, 449 , 456 , 463 , 470 477, 484, 491, 498, 505, 512, 519, 526, 533, 540 , 547 , 554 , 561 568, 575, 582, 589, 596 659C, 666C , 673C, 680C, 687C, 694C , 701C, 708C, 715C, 722C , 72 9C 603C, 610C , 617C, 624C, 631C, 638C , 645C, 652C, 659C, 666C , 673C 680C, 687C , 694C, 701C, 708C, 715C , 722C, 729C 1 , 8 ,. 15, 22 1 , 8 ,. 15, 22, 29, 36, 43 , 50, 57, 64, 71, 78, 85 , 92- 93 1078 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 534 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F4 DOSE: 5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C93086 M 90 APPEARANCE HEAD TILT LEFT SWOLLEN MOUTH NOSE THIN BEHAVIOR ATAXIC <UNSPECIFIED> HYPOACTIVE <UNSPECIFIED> EYES DILATED PUPIL EYES SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE NORMAL NO REMARKABLE OBSERVATIONS 568, 575, 582, 589, 596, 601, 603, 610, 617, 624, 630 601, 603, 610 601, 603, 610 568, 575, 582, 589, 596, 601, 603, 610, 617, 624, 630 630 630 603, 610, 617, 624, 630 601, 603, 610 1, 8, 15 113, 120 204, 211 295, 302 386, 393 477, 484 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 491, 519 1079 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 535 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F4 (CONTINUED FROM PREVIOUS PAGE) C93086 M 90 MASS(ES) INGUINAL REGION-LEFT WEEK 72 VENTRAL-ABDOMINAL-LEFT WEEK 76 C93087 M 100 BEHAVIOR ATAXIC <UNSPECIFIED> HYPOACTIVE <UNSPECIFIED> DISCHARGE NASAL RED IN COLOR EXCRETION FEW FECES EYES RED DISCHARGE EYES SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE NORMAL NO REMARKABLE OBSERVATIONS DOSE: 5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 498C, 505C, 512C 526C, 533C, 54 0C, 54 7C, 554C, 561C, 568C, 575C, 582C, 589C, 596C 601C, 603C, 610C, 617C, 624C, 630C 697 697 694, 697 694, 697 694, 697 1 , 8 , 15, 22, 29, 36, 43,, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0, 127, 134, 141, 148, 155, 162 , 169, 176, 183,. 190, 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281, 288 1080 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 536 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F4 (CONTINUED FROM PREVIOUS PAGE) C93087 M 100 NORMAL NO REMARKABLE OBSERVATIONS C93088 D MASS(ES) INGUINAL REGION-LEFT WEEK 93 INGUINAL REGION-RIGHT WEEK 95 78 APPEARANCE HEAD TILT LEFT THIN EXCRETION FEW FECES EYES RED DISCHARGE EYE-RIGHT HOUSING,FOOD,WATER & MAINT. WATER BOTTLE PLACED ON CAGE SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE ROUGH HAIR COAT DOSE: 5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 295, 302, 309, 316, 323, 330, 337, 344, 351, 358 , 365, 372, 379 386, 393, 400, 407, 414, 421, 428, 435, 442, 449 , 456, 463, 470 477, 484, 491, 498, 505,. 512, 519, 526, 533, 540 , 547, 554, 561 568, 575, 582, 589, 596, 603, 610, 617, 624, 631 , 638 645C, 652C , 659C, 666C, 673C, 680C, 687C , 694C, 697C 659C, 666C, 673C, 680C, 687C, 694C, 697C 527, 533, 540, 544 505-507, 512 506-507, 527 22, 351, 506-507, 512, 519 505 505-507, 512, 527, 533, 540, 544 505-507, 512, 519, 526-527, 533, 540, 544 1081 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 537 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F4 (CONTINUED FROM PREVIOUS PAGE) C93088 D 78 NORMAL NO REMARKABLE OBSERVATIONS C93089 M MASS(ES) AXILLARY REGION-LEFT WEEK 62 WEEK 68 INGUINAL REGION-LEFT WEEK 69 WEEK 72 94 APPEARANCE HEAD TILT LEFT MALOCCLUSION SWOLLEN NOSE NORMAL NO REMARKABLE OBSERVATIONS DOSE: 5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 1 , 8 ,r 15, 29, 36, 43 , 50,r 57, 64, 71, 78 , 85,, 92, 99, 106, 113 1 2 0, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197, 204 2 1 1 , 218, 225,. 232, 239, 246, 253, 260, 267, 274, 281,. 288, 295 302, 309, 316,. 323, 330, 337, 344,. 358, 365, 372, 379,. 386, 393 400, 407, 414, 421, 456, 463, 484, 491 42 8C, 435C, 442C, 449C 470 477 498, 505- 507, 512 652, 155, 246, 337, 428, 519, 610, 656 162, 253, 344, 435, 526, 617, 169, 260, 351, 442, 533, 624, 176, 267, 358, 449, 540, 631, 183, 274, 365, 456, 547, 638, 190, 281, 372, 463, 554, 645, 197, 288, 379, 470, 561, 652, 204, 295, 386, 477, 568, 656 2 1 1 , 218, 302, 309, 393, 400, 484, 491, 575, 582, 225, 316, 407, 498, 589, 232, 323, 414, 505, 596, 239 330 421 512 603 155 1 , 8 , 15, 2 2 , 29, 36, 43,, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0, 127,, 134, 141, 148 1082 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 538 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F4 (CONTINUED FROM PREVIOUS PAGE) C93089 M 94 MASS(ES) AXILLARY REGION-RIGHT WEEK 92 INGUINAL REGION-LEFT WEEK 73 VENTRAL-ABDOMINAL-LEFT WEEK 87 VENTRAL-CERVICAL WEEK 88 C93090 T 105 NORMAL NO REMARKABLE OBSERVATIONS C93091 M 77 APPEARANCE HUNCHED THIN EYES RED DISCHARGE EYES SKIN & PELAGE PALE ENTIRE BODY DOSE: 5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 638C, 64 5C, 652C, 656C 505, 512 603C, 610C , 617C, 624C, 631C, 638C , 645C, 652C, 656C 610, 617, 624, 631C, 638C, 645C, 652C, 656C 1 , 8 , 15, 2 2 , 29, 36 , 43 , 50, 57, 64, 71, 78, 85 , 92, 99, 106 113, 1 2 0, 127,. 134, 141, 148, 155, 162 , 169, 176 , 183, 190 , 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253 , 260, 267 , 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358 , 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449 , 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540 , 547, 554 , 561 568, 575, 582, 589, 596, 603, 610, 617 , 624, 631 , 638, 645 , 652 659, 666, 673, 680, 687, 694, 701, 708 , 715, 722 , 729, 731 512 512, 519, 526, 533-535 512 534-535 1083 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 539 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F4 (CONTINUED FROM PREVIOUS PAGE) C93091 M 77 SKIN & PELAGE SORE/SCAB LATERAL-ABDOMINAL-RIGHT NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) LIMB-HIND-RIGHT WEEK 47 M 77 APPEARANCE HEAD TILT LEFT THIN BEHAVIOR ATAXIC <UNSPECIFIED> EXCRETION FEW FECES EYES RED DISCHARGE EYES DOSE: 5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 534- 535 1 , 8 , 15, 22, 29, 36, 43 , 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253, 260, 267, 274, 281 , 288 295, 302, 309, 316 323C , 330C , 337C, 344C, 351C, 358C , 365C, 372C, 379C, 386C , 393C 400C , 407C , 414C, 421C, 428C, 435C , 442C, 449C, 456C, 463C , 470C 477C , 484C , 491C, 498C, 505C, 512C , 519C, 526C, 533-535C 519, 526, 533, 535 505, 512, 519, 526, 533, 535 533, 535 1084 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 540 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F4 (CONTINUED FROM PREVIOUS PAGE) C93092 M 77 HOUSING,FOOD,WATER & MAINT. WATER BOTTLE PLACED ON CAGE NORMAL NO REMARKABLE OBSERVATIONS C93093 M MASS(ES) AXILLARY REGION-RIGHT WEEK 72 96 APPEARANCE MALOCCLUSION THIN BEHAVIOR HYPOACTIVE <UNSPECIFIED> EXCRETION FEW FECES DOSE: 5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 505 1, 8 , 15 113, 120 204, 211 295, 302 386, 393 477, 484 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 400, 407 , 414, 421, 428, 435 , 442, 449, 456, 463 , 470 491 498C, 505C, 512C, 519C, 526C, 533C, 535C 71, 85, 92, 99,r 106,r 113,r 1 2 0, 127,r 134, 141,r 148,r 155,r 162, 169 176, 183 , 190, 197, 204, 2 1 1 , 218, 225, 232, 239, 246, 253, 260 267, 274 , 281, 288, 295, 302, 309, 316, 323, 330, 337, 344, 351 358, 365 , 372, 379, 386, 393, 400, 407, 414, 421, 428, 435, 442 449, 456 , 463, 470, 477, 484, 491, 498, 505, 512, 519, 526, 533 540, 547 , 554, 561, 568, 575, 582, 589, 596, 603, 610, 617, 624 631, 638 , 645, 652, 659, 666, 670 670 670 670 1085 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 541 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F4 (CONTINUED FROM PREVIOUS PAGE) C93093 M 96 NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) AXILLARY REGION-LEFT WEEK 91 AXILLARY REGION-RIGHT WEEK 87 VENTRAL-ABDOMINAL-LEFT WEEK 70 VENTRAL-ABDOMINAL-RIGHT WEEK 89 VENTRAL-CERVICAL-LEFT WEEK 94 VENTRAL-CERVICAL-RIGHT WEEK 94 M 96 BEHAVIOR HYPOACTIVE <UNSPECIFIED> COLD TO TOUCH ENTIRE BODY EYES RED DISCHARGE EYES SKIN & PELAGE SORE/SCAB VENTRAL-CERVICAL-RIGHT DOSE: 5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 1 , 8 , 15, 22 , 29, 36, 43 , 50, 57, 64 , 78 631C, 638C, 645C, 652C, 659C, 666C, 670C 603C, 610C, 617C, 624C 484C, 491C, 498C, 505C, 512C, 519C, 526C, 533C, 540C 54 7C, 554C 561C, 568C, 575C, 582C, 58 9C, 596C, 603C, 610C, 617C 624C, 631C 638C, 645C, 652C, 659C, 666C, 670C 617C, 624C, 631C, 638C, 645C, 652C, 659C, 666C, 670C 652C, 659C, 666C, 670C 652C, 659C, 666C, 670C 670 670 659, 666, 670 1086 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 542 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F4 (CONTINUED FROM PREVIOUS PAGE) C93094 M 96 NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) VENTRAL-CERVICAL-RIGHT WEEK 76 C93095 D 105 BEHAVIOR HYPOACTIVE <UNSPECIFIED> COLD TO TOUCH ENTIRE BODY EYES PALE EYES SKIN & PELAGE PALE ENTIRE BODY SORE/SCAB AXILLARY REGION-LEFT LATERAL-ABDOMINAL-LEFT DOSE: 5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 1 , 8 , 15, 2 2 , 29, 36, 43 , 50, 57, 64, 71, 78,r 85, 92, 99, 106 113, 1 2 0, 127,. 134, 141, 148, 155, 162, 169, 176, 183, 190 , 197 204, 2 1 1 , 218,. 225, 232, 239, 246, 253, 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344, 351, 358, 365, 372 , 379 386, 393, 400,. 407, 414, 421, 428, 435, 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519 526C, 533C , 540C, 547C, 554C, 561C , 568C, 575C, 582C, 589C , 596C 603C, 610C , 617C, 624C, 631C, 638C , 645C, 652C, 659C, 666C , 670C 729 729 719, 722,729 610, 729 617, 624, 631, 638, 645, 652, 659, 666, 673, 680, 687, 694, 701 708, 715, 719, 722, 729 715, 719 1087 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 543 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F4 (CONTINUED FROM PREVIOUS PAGE) C93095 D 105 NORMAL NO REMARKABLE OBSERVATIONS C93096 D ASS(ES) AXILLARY REGION-LEFT WEEK 79 HEAD-MAXILLARY-RIGHT WEEK 78 INGUINAL REGION-LEFT WEEK 97 SHOULDER-LEFT WEEK 100 VENTRAL-ABDOMINAL WEEK 97 31 NORMAL NO REMARKABLE OBSERVATIONS DOSE: 5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 1 , 8 ,r 15, 2 2 , 29, 36 , 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0, 127,. 134, 141, 148, 155 , 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225, 232, 239, 246 , 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337 , 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428 , 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519 , 526 , 533 547, 554, 561, 568C, 575C , 582C, 589C, 596C, 603C, 610C, 617C 624C, 631C, 638C, 645C, 652C, 659C, 666C, 673C, 680C, 68 7C, 694C 701C, 708C, 715C, 719C, 722C, 72 9C 540 673C, 680C, 687C, 694C, 701C, 708C, 715C, 719C, 722C, 72 9C 694C, 701C, 708 673C, 680C, 687C, 694C, 701C, 708C, 715C, 719C, 722C, 72 9C 1 , 8 , 15, 2 2 , 29, 36, 43,, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 217 1088 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 544 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F4 DOSE: 5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C93097 M 98 BEHAVIOR HYPOACTIVE <UNSPECIFIED> DISCHARGE OTHER SKIN & PELAGE BLACK SKIN VENTRAL-ABDOMINAL-LEFT VENTRAL-THORACIC-LEFT SORE/SCAB VENTRAL-THORACIC-LEFT NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) AXILLARY REGION-LEFT WEEK 69 VENTRAL-ABDOMINAL-LEFT WEEK 63 VENTRAL-CERVICAL WEEK 78 680 680C 659, 666 673, 680 673, 680 1 , 8 , 15, 22, 29, 36, 43 , 50, 57, 64, 71 , 78, 85, 92, 99, 106 113, 1 2 0, 127, 134 , 141, 148, 155, 162, 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225 , 232, 239, 246, 253, 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344, 351, 358, 365, 428 477C, 484C , 491C, 498C, 505C, 512C , 519C , 526C, 533C, 540C , 547C 554C, 561C , 568C, 575C, 582C, 589C , 596C , 603C, 610C, 617C , 624C 631C, 638C , 645C, 652C, 659C, 666C , 673C , 680C 435C, 512C, 58 9C, 666C, 442C , 449C, 519C , 526C, 596C , 603C, 673C , 680C 456C, 533C, 610C, 463C, 54 0C, 617C, 470C , 477C , 484C, 547C , 554C , 561C, 624C , 631C , 638C, 491C, 568C, 645C, 498C , 505C 575C , 582C 652C , 659C 54 0C, 547C , 554C, 561C, 568C, 575C , 582C , 589C, 596C, 603C , 610C 1089 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 545 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F4 (CONTINUED FROM PREVIOUS PAGE) C93097 M 98 MASS(ES) VENTRAL-CERVICAL WEEK 78 VENTRAL-CERVICAL-LEFT WEEK 54 C93098 T 105 NORMAL NO REMARKABLE OBSERVATIONS C93099 M MASS(ES) INGUINAL REGION-LEFT WEEK 100 INGUINAL REGION-RIGHT WEEK 79 96 APPEARANCE HEAD TILT LEFT BEHAVIOR HYPOACTIVE <UNSPECIFIED> DOSE: 5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 617C, 624C, 631C, 638C, 64 5C, 652C, 659C, 666C, 673C, 680C 372C, 379C, 386C, 393C, 400C, 407C, 414C, 421C 1 , 8 ,r 15, 2 2 , 29, 36 , 43,r 50, 113, 1 2 0, 127,. 134, 141, 148, 204, 2 1 1 , 218, 225, 232, 239, 295, 302, 309,. 316, 323, 330, 386, 393, 400, 407, 414, 421, 477, 484, 491, 498, 505, 512, 589, 596, 603,. 610, 617, 624, 680, 687 57, 64, 71, 78, 85, 155, 162 , 169, 176, 246, 253 , 260, 267, 337, 344 , 351, 358, 428, 435 , 442, 449, 519, 526 , 533, 540, 631, 638 , 645, 652, 92, 99, 106 183, 190 , 197 274, 281 , 288 365, 372 , 379 456, 463 , 470 568, 575 , 582 659, 666 , 673 694, 701, 708,. 715, 722, 729, 732 54 7C, 554C, 561C 671 671 1090 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 546 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F4 (CONTINUED FROM PREVIOUS PAGE) C93099 M 96 DISCHARGE GENITAL RED IN COLOR EXCRETION NO FECES SKIN & PELAGE SORE/SCAB VENTRAL-ABDOMINAL-RIGHT NORMAL NO REMARKABLE OBSERVATIONS ASS(ES) AXILLARY REGION-LEFT WEEK 69 WEEK 79 INGUINAL REGION-LEFT WEEK 65 VENTRAL-ABDOMINAL-LEFT WEEK 72 VENTRAL-ABDOMINAL-RIGHT WEEK 72 DOSE: 5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 671 671 526 1 , 8 , 15, 22, 29, 36, 43 , 50, 57, 64, 71, 78,r 85, 92, 99, 106 113, 1 2 0, 127, 134 , 141, 148, 155, 162, 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225 , 232, 239, 246, 253, 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344, 351, 358, 365, 372 , 379 386, 393, 400, 407 , 414, 421, 428, 435, 442, 456, 463, 470 477C, 484C , 491C, 498C, 505C, 512C , 519C, 526C, 533C, 540C , 547C 554C, 561C , 568C, 575C, 582C, 589C 596C, 603C , 610C, 617C, 624C, 631C , 638C, 645C, 652C, 659C , 666C 671C 449C 498C, 505C , 512C, 519C, 526C, 533C , 540C, 547C, 554C, 561C , 568C 575C, 582C , 589C, 596C, 603C, 610C , 617C, 624C, 631C, 638C , 645C 652C, 659C , 666C, 671C 498C, 505C , 512C, 519C, 526C, 533C , 540C, 547C, 554C, 561C , 568C 1091 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 547 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F4 (CONTINUED FROM PREVIOUS PAGE) C93099 M 96 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 72 C93100 D 89 APPEARANCE MALOCCLUSION NORMAL NO REMARKABLE OBSERVATIONS C93101 T MASS(ES) VENTRAL-CERVICAL-RIGHT WEEK 78 105 NORMAL NO REMARKABLE OBSERVATIONS DOSE: 5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 575C 428, 435, 442 1 22, 8,r 15, , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 120113, , 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 211204, , 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 449, 456 , 463, 470, 477, 484 , 491 498, 505, 512, 519, 526, 533, 561, 568 , 575, 582, 589, 596 , 603 610, 617-618 540, 547, 554 221, 8 15, , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 120113, , 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 211204, , 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582, 589, 596, 603, 610, 617 , 624, 631, 638, 645 , 652 666659, , 673, 680, 687, 694, 701, 708 , 715, 722, 729, 732 1092 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 548 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F4 DOSE: 5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C93102 T 105 NORMAL NO REMARKABLE OBSERVATIONS C93103 M MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 42 WEEK 84 VENTRAL -ABDOMINAL-RIGHT WEEK 47 99 APPEARANCE MALOCCLUSION MISSING TEETH BEHAVIOR HYPOACTIVE <UNSPECIFIED> EXCRETION NO FECES SKIN & PELAGE BROWN HAIR COAT VENTRAL-ABDOMINAL 1, 8 , 15, 22, 29, 36, 43, 50, 113, 1 2 0, 127, 134, 141, 148, 204, 2 1 1 , 218, 225, 232, 239, 309, 316, 358, 365, 372, 379, 435, 442, 449, 456, 463, 470, 526, 533, 540, 547, 554, 561, 652, 659, 666, 673, 680, 687, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 386, 393, 400, 407, 477, 484, 491, 498, 568, 575, 617, 624, 694, 701, 708, 715, 92, 99, 10 6 183, 190 197 274, 281 302 414, 421 428 505, 512 519 631, 638 645 722, 729 733 288, 295 582C,. 589C, 596C, 603C, 610C 323C, 330C, 337C, 344C, 351C 519, 589, 596, 603, 610, 617, 624, 631, 638, 645, 652, 659 666 673, 680, 687, 691 687, 691 691 691 691 1093 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 549 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F4 (CONTINUED FROM PREVIOUS PAGE) C93103 M 99 SKIN & PELAGE SORE/SCAB HEAD-MAXILLARY-LEFT PAW-HIND-LEFT PAW-HIND-RIGHT PAWS-HIND NORMAL NO REMARKABLE OBSERVATIONS C93104 M MASS(ES) HEAD-MAXILLARY-LEFT WEEK 76 VENTRAL-CERVICAL-RIGHT WEEK 57 84 APPEARANCE HUNCHED THIN EXCRETION FEW FECES DOSE: 5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 540 568, 659, 547, 638, 505, 575, 582, 589, 666, 673, 680, 554, 561,. 568, 645, 652, 659, 512, 519 596, 603, 687, 691 575, 582, 666, 673, 610, 589, 680, 617 , 624, 596 , 603, 687 , 691 631, 610, 638, 617, 645, 624, 652 631 1 , 8 ,r 15, 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190, 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281, 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372, 379 386, 400, 407,. 414, 421, 428, 435, 442 , 449, 456, 463, 470, 477 484, 491, 498 526, 533 393 561, 568, 575 533, 540, 547, 554, 561, 568, 575 533, 540 1094 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 550 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F4 (CONTINUED FROM PREVIOUS PAGE) C93104 M 84 EYES RED DISCHARGE EYE-RIGHT SKIN & PELAGE RED HAIR COAT VENTRAL-ABDOMINAL SORE/SCAB VENTRAL-THORACIC-LEFT YELLOW HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) AXILLARY REGION-LEFT WEEK 56 INGUINAL REGION-LEFT WEEK 67 VENTRAL-ABDOMINAL-LEFT WEEK 58 VENTRAL-CERVICAL-LEFT WEEK 65 DOSE: 5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 372, 379, 498, 519, 526 568, 575, 582 554, 561, 568,. 575, 582- 583 561 1 , 8 ,. 15, 22, 29, 36, 43 , 50 , 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148 , 155, 162, 169, 176, 183 , 190 , 197 204, 2 1 1 , 218,. 225, 232, 239 , 246, 253, 260, 267, 274 , 281 , 288 295, 302, 309,. 316, 323, 330 , 337, 344, 351, 358, 365 386, 393C, 400C, 407C, 414C, 421C, 428C, 435C, 442C, 44 9C, 456C 463C, 470C, 477C, 484C, 491C , 498C , 505C, 512C, 519C, 526C , 533C 54 0C,. 547C, 554C, 561C, 568C , 575C 463C, 470C, 477C, 484C, 491C , 498C , 505C, 512C, 519C, 526C 400C, 407C, 414C, 421C, 428C , 435C , 442C, 449C, 456C, 463C , 470C 477C,. 484C, 491C, 498C, 505C , 512C , 519C, 526C, 533C, 540C , 547C 554C, 561C, 568C, 575C, 582- 583C 449, 456, 463,. 470, 477 1095 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 551 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F4 DOSE: 5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C93105 D 89 APPEARANCE HUNCHED MALOCCLUSION SWOLLEN LIMB-HIND-RIGHT THIN RESPIRATION LABORED SKIN & PELAGE SORE/SCAB LIMB-HIND-RIGHT PAW-HIND-LEFT PAW-HIND-RIGHT NORMAL NO REMARKABLE OBSERVATIONS M 95 APPEARANCE MISSING TAIL-DISTAL BEHAVIOR HYPOACTIVE <UNSPECIFIED> 589, 596, 603 589, 596, 603,. 610, 617- 618 491, 498, 505,. 512, 519, 526, 533, 540 , 547, 554, 561, 568 , 575 582, 589, 596, 603, 610, 617- 618 589, 596, 603 610 470, 477, 484 498, 505, 519,. 526, 533, 540, 547 449 1 , 8 ,r 15, 2 2 , 29, 36 , 43 , 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 456, 463 554, 561, 568,. 575, 582, 589, 596, 603 , 610, 617, 624, 631 , 638 645, 652, 659, 661 661 1096 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 552 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F4 (CONTINUED FROM PREVIOUS PAGE) C93106 M 95 EXCRETION FEW FECES EYES RED DISCHARGE EYES SKIN & PELAGE SORE/SCAB TAIL-DISTAL NORMAL NO REMARKABLE OBSERVATIONS C93107 T APPEARANCE MALOCCLUSION MISSING TEETH DOSE: 5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 659, 661 659, 661 554, 561, 568, 575, 582, 589, 596 1 , 8 ,r 15, 2 2 , 29, 36 , 43 , 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0, 127, 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547 162, 274, 365, 456, 547, 638, 729, 169, 281, 372, 463, 554, 645, 731 197, 288, 379, 470, 561, 652, 204, 295, 386, 477, 568, 659, 2 1 1 , 218, 302, 309, 393, 400, 484, 491, 575, 582, 666, 673, 225, 316, 407, 498, 589, 680, 232 , 239, 323 , 330, 414 , 421, 505 , 512, 596 , 603, 687 , 694, 246, 337, 428, 519, 610, 701, 253, 344, 435, 526, 617, 708, 260 , 267 351 , 358 442 , 449 533 , 540 624 , 631 715 , 722 428, 435, 442, 449, 456, 463, 470, 477 , 484, 491, 498, 505 , 512 519, 526, 533, 540, 547, 554, 561, 568 , 575, 582, 589, 596 , 603 610, 617, 624, 631, 638, 645, 652, 659 , 666, 673, 680, 687 , 694 701, 708, 715, 722, 729, 731 1097 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 553 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F4 (CONTINUED FROM PREVIOUS PAGE) C93107 T 105 NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) VENTRAL-CERVICAL-RIGHT WEEK 81 C93108 D 52 NORMAL NO REMARKABLE OBSERVATIONS C93109 2 C93110 M 14 NORMAL NO REMARKABLE OBSERVATIONS 90 APPEARANCE THIN BEHAVIOR HYPOACTIVE <UNSPECIFIED> EXCRETION FEW FECES NONFORMED FECES DOSE: 5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS :LISTED AT END OF OBSERVATIONS 1 , 8 ,. 15, 22, 29, 36, 43, 50 , 57, 64, 71, 78,. 85, 92, 99, 106 113, 1 2 0, 127, 134, 141, 148 , 155, 176, 183, 190 561C, 568C , 575C, 582C, 589C , 596C , 603C, 610C, 617C, 624C, 631C 638C, 645C , 652C, 659C, 666C , 673C , 680C, 687C, 694C, 701C, 708C 715C, 722C , 729C, 731C 1 , 8 ,. 15, 22, 29, 36, 43, 50 , 57, 64, 71, 78,. 85, 92, 99, 106 113, 1 2 0, 127, 134, 141, 148 , 155, 162, 169, 176, 183 , 190, 197 204, 2 1 1 , 218, 225, 232, 239 , 246, 253, 260, 267, 274 , 281, 288 295, 302, 309, 316, 323, 330 , 337, 344, 351, 358, 364 1 , 8 ,. 15, 22, 29, 36, 43, 50 , 57, 64, 71, 78,. 85, 92- 93 624, 630 630 630 1098 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 554 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F4 (CONTINUED FROM PREVIOUS PAGE) C93110 M 90 EYES RED DISCHARGE EYE-LEFT EYE-RIGHT SKIN & PELAGE ROUGH HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) AXILLARY REGION-LEFT WEEK 41 AXILLARY REGION-RIGHT WEEK 90 DOSE: 5 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 630 470, 477 624, 630 1 , 8 , 15 113, 120 204, 211 316, 323 407, 414 512, 519 603, 610 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 302 , 309 330, 337 , 344, 351, 358, 365 , 372, 379, 386, 393 , 400 421, 428 , 435, 442, 449, 456 , 463, 484, 491, 498 , 505 526, 533 , 540, 547, 554, 561 , 568, 575, 582, 589 , 596 617 281, 288 295 624C, 63 1099 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 42 MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 42 43 MASS(ES) 44 VENTRAL-CERVICAL-LEFT WEEK 42 45 MASS(ES) 49 VENTRAL-CERVICAL-LEFT WEEK 42 50 MASS(ES) 51 VENTRAL-CERVICAL-LEFT WEEK 42 52 MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 42 53 MASS(ES) 56 VENTRAL-CERVICAL-LEFT WEEK 42 57 MASS(ES) 62 VENTRAL-CERVICAL-LEFT WEEK 42 63 MASS(ES) 66 VENTRAL-CERVICAL-LEFT WEEK 42 20 X 20MM 30 X 25MM 30 X 30MM 40 X 30MM 50 X 40MM 50 X 50MM 60 X 50MM 70 X 55MM 1100 Covance 6329-183 3M T-6295 PAGE: 555 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 71 MASS(ES) 72 INGUINAL REGION-LEFT WEEK 71 95 MASS(ES) 96 INGUINAL REGION-RIGHT WEEK 95 97 MASS(ES) INGUINAL REGION-RIGHT WEEK 95 98 MASS(ES) 99 INGUINAL REGION-RIGHT WEEK 95 99 MASS(ES) 99 VENTRAL-ABDOMINAL WEEK 99 68 MASS(ES) 75 AXILLARY REGION-RIGHT WEEK 68 76 MASS(ES) 78 AXILLARY REGION-RIGHT WEEK 68 79 MASS(ES) 80 AXILLARY REGION-RIGHT WEEK 68 30 X 20MM 20 X 15MM 30 X 25MM 25 X 25MM 20 X 20MM 15 X 15MM 20 X 20MM 30 X 30MM 1101 Covance 6329-183 3M T-6295 PAGE: 556 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C93057 C93063 F4 561 81 TO 589 85 MASS(ES) AXILLARY REGION-RIGHT WEEK 68 596 86 TO 631 91 MASS(ES) AXILLARY REGION-RIGHT WEEK 68 638 92 TO 659 95 MASS(ES) AXILLARY REGION-RIGHT WEEK 68 666 96 TO 691 99 MASS(ES) AXILLARY REGION-RIGHT WEEK 68 540 78 TO 547 79 MASS(ES) VENTRAL-ABDOMINAL WEEK 77 673 97 TO 691 99 MASS(ES) VENTRAL-ABDOMINAL WEEK 97 F4 694 100 MASS(ES) AXILLARY REGION-RIGHT WEEK 100 701 101 MASS(ES) TO 708 102 AXILLARY REGION-RIGHT WEEK 100 40 X 40MM 60 X 40MM 75 X 75MM 75 X 60MM 15 X 15MM 15 X 15MM 10 X 10MM 20 X 20MM 1102 Covance 6329-183 3M T-6295 PAGE: 557 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS CATEGORY KEYWORD WK QUALIFIER FREE-TEXT COMMENT 103 MASS(ES) AXILLARY REGION-RIGHT WEEK 100 104 MASS(ES) AXILLARY REGION-RIGHT WEEK 100 105 MASS(ES) 105 AXILLARY REGION-RIGHT WEEK 100 102 MASS(ES) 103 VENTRAL-CERVICAL WEEK 102 104 MASS(ES) 105 VENTRAL-CERVICAL WEEK 102 84 MASS(ES) 88 AXILLARY REGION-RIGHT WEEK 83 89 MASS(ES) 93 AXILLARY REGION-RIGHT WEEK 83 92 MASS(ES) 93 VENTRAL-ABDOMINAL-RIGHT WEEK 86 20 X 30MM 30 X 30MM 40 X 30MM 20 X 20MM 35 X 35MM 10 X 10MM 15 X 15MM 10 X 10MM 1103 Covance 6329-183 3M T-6295 PAGE: 558 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 72 MASS(ES) 82 VENTRAL-CERVICAL WEEK 72 88 MASS(ES) AXILLARY REGION-LEFT WEEK 84 89 MASS(ES) 91 AXILLARY REGION-LEFT WEEK 84 92 MASS(ES) 93 AXILLARY REGION-LEFT WEEK 84 70 MASS(ES) 73 INGUINAL REGION-LEFT WEEK 70 74 MASS(ES) 87 INGUINAL REGION-LEFT WEEK 70 54 MASS(ES) 56 VENTRAL-ABDOMINAL-LEFT WEEK 54 57 MASS(ES) 59 VENTRAL-ABDOMINAL-LEFT WEEK 54 10 X 10MM 10 X 10MM 20 X 15MM 30 X 20MM 25 X 25MM 40 X 40MM 20 X 10MM 25 X 15MM 1104 Covance 6329-183 3M T-6295 PAGE: 559 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 60 MASS(ES) 62 VENTRAL-ABDOMINAL-LEFT WEEK 54 63 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 54 64 MASS(ES) 66 VENTRAL-ABDOMINAL-LEFT WEEK 54 67 MASS(ES) 69 VENTRAL-ABDOMINAL-LEFT WEEK 54 70 MASS(ES) 76 VENTRAL-ABDOMINAL-LEFT WEEK 54 77 MASS(ES) 80 VENTRAL-ABDOMINAL-LEFT WEEK 54 81 MASS(ES) 82 VENTRAL-ABDOMINAL-LEFT WEEK 54 83 MASS(ES) 84 VENTRAL-ABDOMINAL-LEFT WEEK 54 40 X 20MM 40 X 25MM 50 X 40MM 60 X 45MM 70 X 50MM 80 X 60MM 90 X 55MM 75 X 50MM 1105 Covance 6329-183 3M T-6295 PAGE: 560 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT F4 589 85 TO 603 87 610 88 TO 624 90 631 91 638 92 TO 645 93 484 70 TO 498 72 505 73 TO 561 81 568 82 TO 575 83 582 84 TO 610 88 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 54 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 54 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 54 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 54 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 70 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 70 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 70 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 70 90 X 50MM 110 X 60MM MASSES ON LEFT SIDE ARE MEASURED AS ONE MASS 110 X 90MM MASSES ON LEFT SIDE ARE MEASURED AS ONE MASS 110 X 110MM MASSES ON LEFT SIDE ARE MEASURED AS ONE MASS 10 X 10MM 15 X 15MM 20 X 20MM 30 X 30MM Covance 6329-183 3M T-6295 PAGE: 561 1106 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C93066 C93069 F4 617 89 TO 645 93 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 70 F4 603 87 TO 624 90 MASS(ES) AXILLARY REGION-LEFT WEEK 87 631 91 TO 652 94 MASS(ES) AXILLARY REGION-LEFT WEEK 87 659 95 TO 680 98 MASS(ES) AXILLARY REGION-LEFT WEEK 87 687 99 MASS(ES) TO 694 100 AXILLARY REGION-LEFT WEEK 87 701 101 MASS(ES) TO 702 101 AXILLARY REGION-LEFT WEEK 87 596 86 TO 603 87 MASS(ES) AXILLARY REGION-RIGHT WEEK 86 CO CO 610 MASS(ES) AXILLARY REGION-RIGHT WEEK 86 35 X 35MM 20 X 20MM 30 X 30MM 40 X 30MM 50 X 40MM 60 X 50MM 10 X 10MM 15 X 15MM 1107 Covance 6329-183 3M T-6295 PAGE: 562 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C93069 F4 617 89 TO 631 91 MASS(ES) AXILLARY REGION-RIGHT WEEK 86 638 92 MASS(ES) AXILLARY REGION-RIGHT WEEK 86 645 93 TO 666 96 MASS(ES) AXILLARY REGION-RIGHT WEEK 86 673 97 MASS(ES) TO 702 101 AXILLARY REGION-RIGHT WEEK 86 645 93 TO 666 96 MASS(ES) INGUINAL REGION-LEFT WEEK 93 673 97 TO 687 99 MASS(ES) INGUINAL REGION-LEFT WEEK 93 694 100 MASS(ES) INGUINAL REGION-LEFT WEEK 93 701 101 MASS(ES) TO 702 101 INGUINAL REGION-LEFT WEEK 93 20 X 20MM 25 X 25MM 30 X 25MM 45 X 30MM 30 X 30MM 40 X 35MM 50 X 40MM 50 X 50MM 1108 Covance 6329-183 3M T-6295 PAGE: 563 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C93069 C93072 F4 638 92 TO 659 95 MASS(ES) VENTRAL-THORACIC-RIGHT WEEK 92 666 96 MASS(ES) TO 694 100 VENTRAL-THORACIC-RIGHT WEEK 92 701 101 MASS(ES) TO 702 101 VENTRAL-THORACIC-RIGHT WEEK 92 F4 603 87 MASS(ES) AXILLARY REGION-RIGHT WEEK 87 610 88 MASS(ES) AXILLARY REGION-RIGHT WEEK 87 617 89 TO 652 94 MASS(ES) AXILLARY REGION-RIGHT WEEK 87 659 95 TO 673 97 MASS(ES) AXILLARY REGION-RIGHT WEEK 87 680 98 MASS(ES) AXILLARY REGION-RIGHT WEEK 87 25 X 25MM 30 X 30MM 40 X 35MM 10 X 10MM 15 X 15MM 25 X 25MM 30 X 30MM 35 X 30MM 1109 Covance 6329-183 3M T-6295 PAGE: 564 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C93072 C93076 F4 687 99 MASS(ES) TO 732 105 AXILLARY REGION-RIGHT WEEK 87 715 103 MASS(ES) TO 732 105 INGUINAL REGION-LEFT WEEK 103 F4 582 84 TO 603 87 MASS(ES) AXILLARY REGION-RIGHT WEEK 84 610 TO 624 90 CO CO MASS(ES) AXILLARY REGION-RIGHT WEEK 84 631 91 TO 653 94 MASS(ES) AXILLARY REGION-RIGHT WEEK 84 414 60 TO 428 62 MASS(ES) LIMB-HIND-LEFT WEEK 59 435 63 TO 484 70 MASS(ES) LIMB-HIND-LEFT WEEK 59 491 71 TO 547 79 MASS(ES) LIMB-HIND-LEFT WEEK 59 45 X 40MM 20 X 20MM 30 X 30MM 35 X 40MM 50 X 50MM 55 X 55MM 70 X 65MM 90 X 75MM 1110 Covance 6329-183 3M T-6295 PAGE: 565 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C93076 C93077 C93078 F4 554 80 TO 631 91 MASS(ES) LIMB-HIND-LEFT WEEK 59 638 92 TO 653 94 MASS(ES) LIMB-HIND-LEFT WEEK 59 F4 701 101 MASS(ES) TO 722 104 AXILLARY REGION-LEFT WEEK 101 729 105 MASS(ES) TO 733 105 AXILLARY REGION-LEFT WEEK 101 673 97 TO 687 99 MASS(ES) TAIL-PROXIMAL WEEK 97 694 100 MASS(ES) TO 701 101 TAIL-PROXIMAL WEEK 97 708 102 MASS(ES) TO 733 105 TAIL-PROXIMAL WEEK 97 F4 680 98 MASS(ES) TO 701 101 VENTRAL-ABDOMINAL WEEK 98 110 X 100MM 120 X 125MM 20 X 20MM 30 X 30MM 25 X 20MM 30 X 25MM 25 X 15MM 10 X 10MM 1111 Covance 6329-183 3M T-6295 PAGE: 566 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C93078 F4 652 94 TO 666 96 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 94 673 97 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 94 680 98 TO 687 99 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 94 694 100 MASS(ES) TO 701 101 VENTRAL-ABDOMINAL-LEFT WEEK 94 708 102 MASS(ES) TO 731 105 VENTRAL-ABDOMINAL-LEFT WEEK 94 617 89 TO 624 90 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 89 631 91 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 89 638 92 TO 652 94 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 89 10 X 10MM 15 X 15MM 25 X 20MM 30 X 25MM 40 X 40MM 20 X 20MM 30 X 25MM 40 X 40MM 1112 Covance 6329-183 3M T-6295 PAGE: 567 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C93078 C93079 F4 659 95 TO 666 96 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 89 673 97 TO 680 98 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 89 687 99 MASS(ES) TO 694 100 VENTRAL-ABDOMINAL-RIGHT WEEK 89 701 101 MASS(ES) TO 731 105 VENTRAL-ABDOMINAL-RIGHT WEEK 89 F4 701 101 MASS(ES) TO 732 105 AXILLARY REGION-LEFT WEEK 101 477 69 MASS(ES) AXILLARY REGION-RIGHT WEEK 69 484 70 TO 498 72 MASS(ES) AXILLARY REGION-RIGHT WEEK 69 505 73 TO 519 75 MASS(ES) AXILLARY REGION-RIGHT WEEK 69 50 X 45MM 50 X 50MM 60 X 50MM 70 X 65MM 10 X 10MM 10 X 10MM 20 X 20MM 30 X 25MM 1113 Covance 6329-183 3M T-6295 PAGE: 568 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS STUDY DAY WK CATEGORY KEYWORD QUALIFIER FREE- TEXT C93083 526 76 533 77 MASS(ES) AXILLARY REGION-RIGHT WEEK 69 540 78 547 79 MASS(ES) AXILLARY REGION-RIGHT WEEK 69 554 80 582 84 MASS(ES) AXILLARY REGION-RIGHT WEEK 69 589 85 631 91 MASS(ES) AXILLARY REGION-RIGHT WEEK 69 638 92 MASS(ES) > 694 100 AXILLARY REGION-RIGHT WEEK 69 701 101 MASS(ES) 732 105 AXILLARY REGION-RIGHT WEEK 69 F4 659 95 MASS(ES) > 729 105 VENTRAL-ABDOMINAL WEEK 95 603 87 MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 87 30 X 30MM 30 X 40MM 40 X 40MM 40 X 55MM 60 X 70MM 70 X 80MM 15 X 10MM 15 X 15MM 1114 Covance 6329-183 3M T-6295 PAGE: 569 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C93083 F4 610 88 TO 624 90 MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 87 631 91 MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 87 638 92 TO 652 94 MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 87 659 95 TO 673 97 MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 87 680 98 MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 87 687 99 MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 87 694 100 MASS(ES) TO 722 104 VENTRAL-CERVICAL-LEFT WEEK 87 729 105 MASS(ES) TO 729 105 VENTRAL-CERVICAL-LEFT WEEK 87 30 X 30MM 50 X 45MM 60 X 55MM 70 X 65MM 70 X 75MM 80 X 75MM 90 X 90MM 100 X 90MM 1115 Covance 6329-183 3M T-6295 PAGE: 570 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 72 MASS(ES) 74 INGUINAL REGION-LEFT WEEK 72 76 MASS(ES) 77 VENTRAL-ABDOMINAL-LEFT WEEK 76 78 MASS(ES) 82 VENTRAL-ABDOMINAL-LEFT WEEK 76 83 MASS(ES) 85 VENTRAL-ABDOMINAL-LEFT WEEK 76 86 MASS(ES) 88 VENTRAL-ABDOMINAL-LEFT WEEK 76 89 MASS(ES) 90 VENTRAL-ABDOMINAL-LEFT WEEK 76 93 MASS(ES) INGUINAL REGION-LEFT WEEK 93 94 MASS(ES) 99 INGUINAL REGION-LEFT WEEK 93 15 X 15MM 30 X 30MM 40 X 30MM 40 X 40MM 50 X 40MM 60 X 50MM 20 X 20MM 30 X 30MM 1116 Covance 6329-183 3M T-6295 PAGE: 571 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE- TEXT C93087 C93088 C93089 C93091 F4 694 100 MASS(ES) TO 697 100 INGUINAL REGION-LEFT WEEK 93 659 95 MASS(ES) TO 697 100 INGUINAL REGION-RIGHT WEEK 95 F4 428 62 TO 449 65 MASS(ES) AXILLARY REGION-LEFT WEEK 62 F4 638 92 TO 656 94 MASS(ES) AXILLARY REGION-RIGHT WEEK 92 603 87 TO 656 94 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 87 631 91 TO 645 93 MASS(ES) VENTRAL-CERVICAL WEEK 88 652 94 TO 656 94 MASS(ES) VENTRAL-CERVICAL WEEK 88 F4 323 47 MASS(ES) LIMB-HIND-RIGHT WEEK 47 40 X 30MM 20 X 20MM 10 X 10MM 15 X 15MM 15 X 15MM 20 X 20MM 25 X 25MM 20 X 15MM 1117 Covance 6329-183 3M T-6295 PAGE: 572 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 48 MASS(ES) 51 LIMB-HIND-RIGHT WEEK 47 52 MASS(ES) LIMB-HIND-RIGHT WEEK 47 53 MASS(ES) 55 LIMB-HIND-RIGHT WEEK 47 56 MASS(ES) 60 LIMB-HIND-RIGHT WEEK 47 61 MASS(ES) LIMB-HIND-RIGHT WEEK 47 62 MASS(ES) LIMB-HIND-RIGHT WEEK 47 63 MASS(ES) 64 LIMB-HIND-RIGHT WEEK 47 65 MASS(ES) LIMB-HIND-RIGHT WEEK 47 25 X 25MM 25 X 15MM 25 X 30MM 30 X 30MM 40 X 40MM 30 X 30MM 30 X 40MM 60 X 50MM 1118 Covance 6329-183 3M T-6295 PAGE: 573 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 66 MASS(ES) 69 LIMB-HIND-RIGHT WEEK 47 70 MASS(ES) 71 LIMB-HIND-RIGHT WEEK 47 72 MASS(ES) 76 LIMB-HIND-RIGHT WEEK 47 77 MASS(ES) 77 LIMB-HIND-RIGHT WEEK 47 72 MASS(ES) 77 AXILLARY REGION-RIGHT WEEK 72 91 MASS(ES) 96 AXILLARY REGION-LEFT WEEK 91 87 MASS(ES) 90 AXILLARY REGION-RIGHT WEEK 87 70 MASS(ES) 72 VENTRAL-ABDOMINAL-LEFT WEEK 70 40 X 40MM 50 X 50MM 70 X 70MM 100 X 100MM 10 X 10MM 10 X 10MM 10 X 10MM 30 X 20MM 1119 Covance 6329-183 3M T-6295 PAGE: 574 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 73 MASS(ES) 75 VENTRAL-ABDOMINAL-LEFT WEEK 70 76 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 70 77 MASS(ES) 83 VENTRAL-ABDOMINAL-LEFT WEEK 70 84 MASS(ES) 87 VENTRAL-ABDOMINAL-LEFT WEEK 70 88 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 70 89 MASS(ES) 91 VENTRAL-ABDOMINAL-LEFT WEEK 70 92 MASS(ES) 94 VENTRAL-ABDOMINAL-LEFT WEEK 70 95 MASS(ES) 96 VENTRAL-ABDOMINAL-LEFT WEEK 70 40 X 30MM 40 X 40MM 60 X 60MM 65 X 60MM 70 X 70MM 75 X 75MM 80 X 80MM 70 X 70MM 1120 Covance 6329-183 3M T-6295 PAGE: 575 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 89 MASS(ES) 91 VENTRAL-ABDOMINAL-RIGHT WEEK 89 92 MASS(ES) 96 VENTRAL-ABDOMINAL-RIGHT WEEK 89 94 MASS(ES) 96 VENTRAL-CERVICAL-LEFT WEEK 94 94 MASS(ES) 96 VENTRAL-CERVICAL-RIGHT WEEK 94 76 MASS(ES) 79 VENTRAL-CERVICAL-RIGHT WEEK 76 80 MASS(ES) 81 VENTRAL-CERVICAL-RIGHT WEEK 76 82 MASS(ES) VENTRAL-CERVICAL-RIGHT WEEK 76 83 MASS(ES) 84 VENTRAL-CERVICAL-RIGHT WEEK 76 25 X 25MM 45 X 45MM 15 X 15MM 10 X 10MM 30 X 30MM 20 X 20MM 10 X 10MM 20 X 20MM 1121 Covance 6329-183 3M T-6295 PAGE: 576 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 85 MASS(ES) 86 VENTRAL-CERVICAL-RIGHT WEEK 76 87 MASS(ES) 90 VENTRAL-CERVICAL-RIGHT WEEK 76 91 MASS(ES) VENTRAL-CERVICAL-RIGHT WEEK 76 92 MASS(ES) 93 VENTRAL-CERVICAL-RIGHT WEEK 76 94 MASS(ES) 96 VENTRAL-CERVICAL-RIGHT WEEK 76 82 MASS(ES) 83 AXILLARY REGION-LEFT WEEK 79 84 MASS(ES) 86 AXILLARY REGION-LEFT WEEK 79 87 MASS(ES) 93 AXILLARY REGION-LEFT WEEK 79 30 X 30MM 40 X 35MM 50 X 35MM 55 X 45MM 55 X 60MM 10 X 10MM 15 X 15MM 25 X 25MM 1122 Covance 6329-183 3M T-6295 PAGE: 577 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS CATEGORY KEYWORD WK QUALIFIER FREE-TEXT COMMENT 94 MASS(ES) 95 AXILLARY REGION-LEFT WEEK 79 96 MASS(ES) 99 AXILLARY REGION-LEFT WEEK 79 100 MASS(ES) 101 AXILLARY REGION-LEFT WEEK 79 102 MASS(ES) AXILLARY REGION-LEFT WEEK 79 103 MASS(ES) 105 AXILLARY REGION-LEFT WEEK 79 97 MASS(ES) 99 INGUINAL REGION-LEFT WEEK 97 100 MASS(ES) 101 INGUINAL REGION-LEFT WEEK 97 102 MASS(ES) INGUINAL REGION-LEFT WEEK 97 20 X 20MM 20 X 30MM 30 X 25MM 35 X 30MM 40 X 35MM 20 X 20MM 30 X 30MM 30 X 45MM 1123 Covance 6329-183 3M T-6295 PAGE: 578 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C93095 C93097 F4 715 103 MASS(ES) TO 719 103 INGUINAL REGION-LEFT WEEK 97 722 104 MASS(ES) TO 729 105 INGUINAL REGION-LEFT WEEK 97 694 100 MASS(ES) TO 701 101 SHOULDER-LEFT WEEK 100 673 97 TO 687 99 MASS(ES) VENTRAL-ABDOMINAL WEEK 97 694 100 MASS(ES) TO 729 105 VENTRAL-ABDOMINAL WEEK 97 F4 680 98 DISCHARGE TO 680 98 OTHER 477 69 MASS(ES) AXILLARY REGION-LEFT WEEK 69 484 70 TO 498 72 MASS(ES) AXILLARY REGION-LEFT WEEK 69 40 X 50MM 55 X 40MM 10 X 10MM 10 X 10MM 20 X 20MM AXILLARY LEFT MASS BLEEDING 10 X 10MM 20 X 30MM 1124 Covance 6329-183 3M T-6295 PAGE: 579 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 73 MASS(ES) 75 AXILLARY REGION-LEFT WEEK 69 76 MASS(ES) 79 AXILLARY REGION-LEFT WEEK 69 80 MASS(ES) 90 AXILLARY REGION-LEFT WEEK 69 91 MASS(ES) AXILLARY REGION-LEFT WEEK 69 92 MASS(ES) 98 AXILLARY REGION-LEFT WEEK 69 63 MASS(ES) 65 VENTRAL-ABDOMINAL-LEFT WEEK 63 66 MASS(ES) 72 VENTRAL-ABDOMINAL-LEFT WEEK 63 73 MASS(ES) 74 VENTRAL-ABDOMINAL-LEFT WEEK 63 30 X 30MM 20 X 20MM 30 X 30MM 40 X 40MM 55 X 60MM 15 X 15MM 25 X 20M 40 X 25MM 1125 Covance 6329-183 3M T-6295 PAGE: 580 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 75 MASS(ES) 77 VENTRAL-ABDOMINAL-LEFT WEEK 63 78 MASS(ES) 80 VENTRAL-ABDOMINAL-LEFT WEEK 63 81 MASS(ES) 87 VENTRAL-ABDOMINAL-LEFT WEEK 63 88 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 63 89 MASS(ES) 93 VENTRAL-ABDOMINAL-LEFT WEEK 63 94 MASS(ES) 95 VENTRAL-ABDOMINAL-LEFT WEEK 63 96 MASS(ES) 98 VENTRAL-ABDOMINAL-LEFT WEEK 63 78 MASS(ES) VENTRAL-CERVICAL WEEK 78 30 X 20MM 30 X 30MM 40 X 30MM 40 X 40MM 40 X 50MM 40 X 35MM 40 X 35MM 15 X 15MM 1126 Covance 6329-183 3M T-6295 PAGE: 581 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 79 MASS(ES) 84 VENTRAL-CERVICAL WEEK 78 85 MASS(ES) VENTRAL-CERVICAL WEEK 78 86 MASS(ES) 88 VENTRAL-CERVICAL WEEK 78 89 MASS(ES) 90 VENTRAL-CERVICAL WEEK 78 91 MASS(ES) 92 VENTRAL-CERVICAL WEEK 78 93 MASS(ES) 98 VENTRAL-CERVICAL WEEK 78 54 MASS(ES) 61 VENTRAL-CERVICAL-LEFT WEEK 54 79 MASS(ES) 81 INGUINAL REGION-RIGHT WEEK 79 20 X 20MM 30 X 30MM 40 X 30MM 50 X 40MM 40 X 40MM 50 X 50MM 15 X 15MM 20 X 20MM 1127 Covance 6329-183 3M T-6295 PAGE: 582 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 69 MASS(ES) 80 AXILLARY REGION-LEFT WEEK 69 81 MASS(ES) 85 AXILLARY REGION-LEFT WEEK 69 86 MASS(ES) 92 AXILLARY REGION-LEFT WEEK 79 93 MASS(ES) AXILLARY REGION-LEFT WEEK 79 94 MASS(ES) AXILLARY REGION-LEFT WEEK 79 95 MASS(ES) 96 AXILLARY REGION-LEFT WEEK 79 65 MASS(ES) INGUINAL REGION-LEFT WEEK 65 72 MASS(ES) 80 VENTRAL-ABDOMINAL-LEFT WEEK 72 10 X 10MM 15 X 15MM 20 X 20MM 30 X 25MM 20 X 20MM 30 X 20MM 10 X 10MM 15 X 15MM 1128 Covance 6329-183 3M T-6295 PAGE: 583 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 81 MASS(ES) 87 VENTRAL-ABDOMINAL-LEFT WEEK 72 88 MASS(ES) 93 VENTRAL-ABDOMINAL-LEFT WEEK 72 94 MASS(ES) 96 VENTRAL-ABDOMINAL-LEFT WEEK 72 72 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 72 73 MASS(ES) 83 VENTRAL-ABDOMINAL-RIGHT WEEK 72 84 MASS(ES) 88 VENTRAL-ABDOMINAL-LEFT WEEK 84 47 MASS(ES) 51 VENTRAL-ABDOMINAL-RIGHT WEEK 47 57 MASS(ES) 61 AXILLARY REGION-LEFT WEEK 56 20 X 20MM 30 X 25MM 20 X 20MM 20 X 20MM 15 X 15MM 10 X 10MM 10 X 10MM 10 X 10MM 1129 Covance 6329-183 3M T-6295 PAGE: 584 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 62 MASS(ES) 63 AXILLARY REGION-LEFT WEEK 56 64 MASS(ES) 65 AXILLARY REGION-LEFT WEEK 56 66 MASS(ES) 76 AXILLARY REGION-LEFT WEEK 56 77 MASS(ES) AXILLARY REGION-LEFT WEEK 56 78 MASS(ES) 80 AXILLARY REGION-LEFT WEEK 56 81 MASS(ES) 83 AXILLARY REGION-LEFT WEEK 56 67 MASS(ES) 73 INGUINAL REGION-LEFT WEEK 67 74 MASS(ES) 76 INGUINAL REGION-LEFT WEEK 67 15 X 20MM 20 X 25MM 30 X 35MM 40 X 40MM 20 X 40MM 15 X 35MM 15 X 15MM 20 X 20MM 1130 Covance 6329-183 3M T-6295 PAGE: 585 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 58 MASS(ES) 59 VENTRAL-ABDOMINAL-LEFT WEEK 58 60 MASS(ES) 61 VENTRAL-ABDOMINAL-LEFT WEEK 58 62 MASS(ES) 67 VENTRAL-ABDOMINAL-LEFT WEEK 58 68 MASS(ES) 69 VENTRAL-ABDOMINAL-LEFT WEEK 58 70 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 58 71 MASS(ES) 73 VENTRAL-ABDOMINAL-LEFT WEEK 58 74 MASS(ES) 75 VENTRAL-ABDOMINAL-LEFT WEEK 58 76 MASS(ES) 84 VENTRAL-ABDOMINAL-LEFT WEEK 58 15 X 15MM 20 X 20MM 30 X 25MM 30 X 30MM 70 X 40MM 40 X 30MM 60 X 40MM 45 X 30MM 1131 Covance 6329-183 3M T-6295 PAGE: 586 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C93107 F4 561 81 TO 603 87 MASS(ES) VENTRAL-CERVICAL-RIGHT WEEK 81 610 88 TO 624 90 MASS(ES) VENTRAL-CERVICAL-RIGHT WEEK 81 631 91 MASS(ES) VENTRAL-CERVICAL-RIGHT WEEK 81 638 92 TO 645 93 MASS(ES) VENTRAL-CERVICAL-RIGHT WEEK 81 652 94 TO 687 99 MASS(ES) VENTRAL-CERVICAL-RIGHT WEEK 81 694 100 MASS(ES) TO 701 101 VENTRAL-CERVICAL-RIGHT WEEK 81 708 102 MASS(ES) VENTRAL-CERVICAL-RIGHT WEEK 81 715 103 MASS(ES) TO 731 105 VENTRAL-CERVICAL-RIGHT WEEK 81 30 X 30MM 40 X 30MM 50 X 30MM 50 X 50MM 60 X 60MM 80 X 60MM 70 X 60 MM 70 X 80MM 1132 Covance 6329-183 3M T-6295 PAGE: 587 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C93110 F4 624 90 TO 630 90 MASS(ES) AXILLARY REGION-RIGHT WEEK 90 20 X 20MM Covance 6329-183 3M T-6295 PAGE: 588 1133 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 589 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F5 DOSE: 20 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C93111 2 C93112 3 14 NORMAL NO REMARKABLE OBSERVATIONS 53 EYES RED DISCHARGE EYE-LEFT NORMAL NO REMARKABLE OBSERVATIONS C93113 T SURGERY PERFORMED BY PATTIE MURPHY ANTIBIOTIC ADMINISTERED 0.5 ML 105 EYES SCAB EYE-RIGHT NORMAL NO REMARKABLE OBSERVATIONS 1, 8 , 15, 22, 29, 36, 43, 50 57, 64, 71, 78 85 92-93 316 1 , 8 ,r 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127, 134 , 141, 148, 155, 162 , 169, 176, 183,. 190, 197 204, 2 1 1 , 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281, 288 295, 302, 309, 323 , 330, 337, 344, 351 , 358, 365- 366 359 359-360 554 1 , 8 ,r 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400 407 , 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491 498 , 519, 526, 533, 540 , 547, 561, 568, 575 , 582 1134 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 590 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F5 (CONTINUED FROM PREVIOUS PAGE) C93113 T 105 NORMAL NO REMARKABLE OBSERVATIONS C93114 1 C93115 T MASS(ES) INGUINAL REGION-RIGHT WEEK 73 VENTRAL-ABDOMINAL-LEFT WEEK 103 4 NORMAL NO REMARKABLE OBSERVATIONS 105 SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) INGUINAL REGION-LEFT WEEK 73 DOSE: 20 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 589, 596, 603, 610, 617, 624, 631, 638, 645, 652, 659, 666, 673 680, 687, 694, 701, 708 505, 512 715C, 722C, 729C, 731C 1, 8 , 15, 22 393 1 , 8 ,r 15, 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 400, 407 414, 421, 428, 435, 442 , 449, 456, 463, 470 , 477 484, 491, 498 519, 526, 533, 540, 547 , 554, 561, 568, 575 , 582 589, 596, 603 610, 617, 624, 631, 638 , 645, 652, 659, 666 , 673 680, 687, 694 701, 708, 715, 722, 729 , 732 505, 512 1135 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 591 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F5 DOSE: 20 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C93116 3 53 NORMAL NO REMARKABLE OBSERVATIONS C93117 3 53 NORMAL NO REMARKABLE OBSERVATIONS C93118 M 93 APPEARANCE MALOCCLUSION MISSING TEETH THIN EYES PROTRUDING EYES SKIN & PELAGE SORE/SCAB MOUTH VENTRAL-ABDOMINAL-LEFT 1 , 8 ,r 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365- 366 1 , 8 ,r 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365- 366 190, 197, 204, 211 , 218, 225, 232, 239 , 246, 253, 260, 267 , 274 281, 400, 407, 414 , 421, 428, 435, 442 , 449, 456, 463, 470 , 477 484, 491, 498, 505 , 512, 519, 526, 533 , 540, 547, 554, 561 , 568 575, 582, 589, 596 , 603, 610, 617, 624 , 631, 638, 645 624, 631, 638, 645 491, 498, 505, 512, 519, 526, 533, 540, 547, 554, 561, 568, 575 582, 589, 596, 603, 610, 617, 624, 631, 638, 645 512 99 624, 631, 638, 645 1136 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 592 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F5 (CONTINUED FROM PREVIOUS PAGE) C93118 M 93 NORMAL NO REMARKABLE OBSERVATIONS ASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 49 C93119 M VENTRAL-ABDOMINAL-RIGHT WEEK 53 92 APPEARANCE MALOCCLUSION EYES DISCOLORED/LIGHT RED EYE-RIGHT RESPIRATION AUDIBLE SKIN & PELAGE ROUGH HAIR COAT SORE/SCAB DORSAL MOUTH DOSE: 20 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 1 , 8 , 15, 22, 29, 36, 43 , 50, 57, 64, 71 , 78, 85, 92, 106, 113 1 2 0 , 127, 134, 141 , 148, 155, 162, 169, 176, 183, 288, 295, 302 309, 316, 323, 330 337C, 414C, 491C, 568C, 645C 344C , 351C, 421C , 428C, 498C , 505C, 575C , 582C, 358C, 435C, 512C, 58 9C, 365C, 442C, 519C, 596C, 372C , 379C , 386C, 449C , 456C , 463C, 526C , 533C , 540C, 603C , 610C , 617C, 393C, 470C, 547C, 624C, 400C, 477C, 554C, 631C, 407C 484C 561C 638C 365C, 372C , 379C, 386C, 393C, 400C , 407C , 414C, 421C, 428C, 435C 441- 442, 449, 456, 463, 470, 477, 484, 491, 498, 505, 512, 519 526, 533,, 540,. 547, 554, 561, 568, 575, 582, 589,. 596, 603, 610 617, 624, 631, 638 505, 512 441- 442, 449 554, 561,, 568,. 575, 582, 589, 596, 603, 610, 617,. 624, 631, 638 617, 624, 631, 638 449 1137 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 593 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F5 (CONTINUED FROM PREVIOUS PAGE) C93119 M 92 SKIN & PELAGE SORE/SCAB SHOULDER-RIGHT NORMAL NO REMARKABLE OBSERVATIONS 3 53 NORMAL NO REMARKABLE OBSERVATIONS SURGERY PERFORMED BY KATHY EWERS ANTIBIOTIC ADMINISTERED 0.5 ML DOSE: 20 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 547, 554, 561, 568 , 575, 582, 589, 596 , 603, 610, 617, 624 , 631 638 1 , 8 ,r 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407 , 414, 421, 428, 435 1 , 8 ,r 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365- 366 359 359-360 1138 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 594 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F5 DOSE: 20 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C93121 M 79 APPEARANCE HEAD TILT LEFT THIN TREMORS ENTIRE BODY BEHAVIOR HYPOACTIVE <UNSPECIFIED> COLD TO TOUCH ENTIRE BODY EXCRETION FEW FECES SKIN & PELAGE ROUGH HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS 547, 549 505, 512, 519, 526, 533, 540, 547, 549 549 549 549 512, 526, 533, 540 519, 526, 533,. 540 , 547, 549 1 , 8 ,r 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407 , 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498 1139 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 595 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F5 DOSE: 20 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C93122 M 92 APPEARANCE THIN BEHAVIOR HYPOACTIVE <UNSPECIFIED> EXCRETION DISCOLORED FECES YELLOW IN COLOR FEW FECES NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) INGUINAL REGION-LEFT WEEK 71 INGUINAL REGION-RIGHT WEEK 72 WEEK 78 VENTRAL-ABDOMINAL-LEFT WEEK 71 642 642 642 641- 642 1 , 8 , 15, 22, 29, 36, 43 , 50 , 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148 , 155, 162, 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239 , 246, 253, 260, 267, 274, 281 , 288 295, 302, 309, 316, 323, 330 , 337, 344, 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421 , 428, 435, 442, 449, 456 491, 498, 505, 512C 498C , 505C, 575C , 582C, 641- 642C 540C , 547C, 617C , 624C, 512C, 589C, 554C, 631C, 519C, 596C, 561C, 638C, 526C , 533C , 540C, 603C , 610C , 617C, 568C , 575C , 582C, 641- 642C 547C, 624C, 589C, 554C, 631C, 596C, 561C , 568C 638C 603C , 610C 519C , 526C, 533C, 540C, 547C , 554C , 561C, 568C, 575C, 582C , 589C 596C , 603C, 610C, 617C, 624C , 631C , 638C, 641-642C 1140 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 596 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F5 (CONTINUED FROM PREVIOUS PAGE) C93122 M 92 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 67 C93123 1 C93124 T 4 NORMAL NO REMARKABLE OBSERVATIONS 105 APPEARANCE THIN SKIN & PELAGE BLUE SKIN VENTRAL-ABDOMINAL-LEFT BLACK SKIN VENTRAL-ABDOMINAL-LEFT PALE ENTIRE BODY SORE/SCAB VENTRAL-ABDOMINAL VENTRAL-ABDOMINAL-LEFT VENTRAL-ABDOMINAL-RIGHT NORMAL NO REMARKABLE OBSERVATIONS DOSE: 20 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 463C, 470C, 477C, 484C, 491C, 498C, 505C, 512C, 519C, 52 6C, 533C 54 0C, 54 7C, 554C, 561C, 568C, 575C, 582C, 589C, 596C, 603C, 610C 617C, 624C , 631C, 638C, 641- 642C 1 , 8 , 15, 22 673, 680, 687,. 694 , 701, 708 , 715, 722 , 729, 732 729, 732 596, 603, 610, 617 , 624, 631 , 638 589 666, 673, 680, 687 , 694 610, 617, 624, 631 , 638, 645 , 652, 659 , 666, 673, 680, 687 , 694 701, 708, 715, 722 , 729, 732 729, 732 1 , 8 , 15, 2 2 , 29, 36, 43 , 50 , 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127, 134 , 141, 148 , 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225 , 232, 239 , 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330 , 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407 , 414, 421 , 428, 435 , 442, 449, 456, 463 , 470 477, 484 1141 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 597 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F5 (CONTINUED FROM PREVIOUS PAGE) C93124 T 105 MASS(ES) AXILLARY REGION-LEFT WEEK 73 WEEK 94 WEEK 102 DORSAL WEEK 76 INGUINAL REGION-LEFT WEEK 76 INGUINAL REGION-RIGHT WEEK 72 SHOULDER-LEFT WEEK 95 VENTRAL-ABDOMINAL-LEFT WEEK 71 VENTRAL-ABDOMINAL-RIGHT WEEK 72 WEEK 85 WEEK 94 VENTRAL-CERVICAL-LEFT WEEK 102 VENTRAL-THORACIC-RIGHT WEEK 71 DOSE: 20 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 505, 512, 519, 526C, 533C, 540C, 547C, 554C, 561C, 568C, 575C 582C, 589C , 596C, 603C, 610C, 617C 652C, 659C 708C, 715C , 722C, 72 9C, 732C 52 6C, 533C , 540C, 54 7C, 554C, 561C, 568C, 575C, 582C 52 6C, 533C , 540C, 54 7C, 554C, 561C, 568C, 575C, 582C 498C, 505C, 512C 659C, 666C, 673C, 680C, 687C, 694C, 701C, 708C, 715C, 722C, 72 9C 732C 491C, 498C, 505C, 512C, 519C, 526C, 533C, 54 0C, 54 7C, 554C, 561C 568C, 575C, 582C, 58 9C, 596C, 603C, 610C, 617C, 624C, 631C, 638C 645C, 652C, 659C, 666C, 673C, 680C, 687C, 694C, 701C, 708C, 715C 722C, 729C, 732C 519C, 596C, 673C, 589C 652C, 729C, 526C, 603C, 680C, 659C, 732C 533C, 610C, 687C, 666C, 54 0C, 617C, 694C, 673C, 54 7C, 624C, 701C, 680C, 554C, 631C, 708C, 687C, 561C, 638C, 715C, 694C, 568C, 645C, 722C, 701C, 575C, 652C, 729C, 708C, 582C, 659C, 732C 715C, 589C 666C 722C 708C, 715C, 722C, 72 9C, 732C 491C, 498C, 505C, 512C, 519C, 526C, 533C, 54 0C, 54 7C, 554C, 561C 568C, 575C, 582C, 58 9C, 596C, 603C, 610C, 617C 1142 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 598 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F5 (CONTINUED FROM PREVIOUS PAGE) C93124 T 105 MASS(ES) VENTRAL-THORACIC-RIGHT WEEK 97 T 105 NORMAL NO REMARKABLE OBSERVATIONS C93126 M 58 APPEARANCE THIN BEHAVIOR HYPOACTIVE MODERATE RECUMBENT LATERAL DISCHARGE NASAL RED IN COLOR EYES RED DISCHARGE EYES DOSE: 20 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 673C, 680C , 687C, 694C, 701C, 708C , 715C , 722C, 729C, 732C 1 , 8 , 15, 2 2 , 29, 36, 43 , 50, 57, 64, 71 , 78, 85 , 92, 99, 106 113, 1 2 0 , 127, 134 , 141, 148, 155, 162, 169, 176 , 183, 190 , 197 204, 2 1 1 , 218, 225 , 232, 239, 246, 253, 260, 267 , 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344, 351, 358 , 365, 372 , 379 386, 393, 400, 407 , 414, 421, 428, 435, 442, 449 , 456, 463 , 470 477, 484, 491, 498 , 505, 512, 519, 526, 533, 540 , 547, 554 , 561 568, 575, 582, 589 , 596, 603, 610, 617, 624, 631 , 638, 645 , 652 659, 666, 673, 680 , 687, 694, 701, 708, 715, 722 , 729, 731 400, 406 406 406 406 406 1143 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 599 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F5 (CONTINUED FROM PREVIOUS PAGE) C93126 M 58 SKIN & PELAGE ROUGH HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS C93127 2 C93128 M MASS(ES) VENTRAL-THORACIC-LEFT WEEK 47 14 NORMAL NO REMARKABLE OBSERVATIONS 100 BEHAVIOR HYPOACTIVE <UNSPECIFIED> EYES RED DISCHARGE EYES RESPIRATION LABORED SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE DOSE: 20 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 400, 406 221, 8, 15, , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 330, 337, 344, 351 , 358, 365, 372, 379 , 386 393 323 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92-93 694 694 694 512, 519, 526, 533, 540, 547, 554 1144 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 600 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F5 (CONTINUED FROM PREVIOUS PAGE) C93128 M 100 NORMAL NO REMARKABLE OBSERVATIONS C93129 D MASS(ES) INGUINAL REGION-RIGHT WEEK 74 100 EYES DILATED PUPIL EYES DISCOLORED/LIGHT RED EYES PROTRUDING EYES SKIN & PELAGE SORE/SCAB MOUTH PAW-HIND-RIGHT NORMAL NO REMARKABLE OBSERVATIONS DOSE: 20 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 1 , 8 ,r 15, 2 2 , 29, 36, 43,r 50, 113, 1 2 0, 127, 134 , 141, 148, 204, 2 1 1 , 218, 225 , 232, 239, 295, 302, 309, 316 , 323, 330, 386, 393, 400, 407 , 414, 421, 477, 484, 491, 498 , 505, 561, 617, 624, 631, 638 , 645, 652, 57, 64, 71, 78, 85, 155, 162 , 169, 176, 246, 253 , 260, 267, 337, 344 , 351, 358, 428, 435 , 442, 449, 568, 575 , 582, 589, 659, 666 , 673, 680, 92, 99, 106 183, 190 , 197 274, 281 , 288 365, 372 , 379 456, 463 , 470 596, 603 , 610 687 512 610, 617, 624, 631, 638, 645, 652, 659, 666, 673, 680, 687, 694 666, 673, 680, 687, 694 491, 498, 505, 512, 519, 526, 533, 540, 547, 554, 561, 568, 575 582, 589, 596, 603, 610, 617, 624, 631, 638, 645, 652, 659, 666 673, 680, 687, 694 554 554, 561, 568 1 , 8 , 15, 22, 29, 36, 43,, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0, 127, 134, 141, 148, 155, 162 , 169, 176, 183,. 190, 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281, 288 1145 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 601 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F5 (CONTINUED FROM PREVIOUS PAGE) C93129 D 100 NORMAL NO REMARKABLE OBSERVATIONS C93130 3 53 NORMAL NO REMARKABLE OBSERVATIONS C93131 M 96 APPEARANCE HUNCHED THIN EYES RED DISCHARGE EYES SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE NORMAL NO REMARKABLE OBSERVATIONS DOSE: 20 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470 477, 484 1 , 8 ,r 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190, 197 204, 2 1 1 , 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281, 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365-366 666 666 652, 666 652 1 , 8 , 15 113, 120 204, 211 295, 302 386, 393 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 1146 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 602 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F5 (CONTINUED FROM PREVIOUS PAGE) C93131 M 96 MASS(ES) AXILLARY REGION-LEFT WEEK 91 AXILLARY REGION-RIGHT WEEK 68 INGUINAL REGION-RIGHT WEEK 89 VENTRAL-ABDOMINAL-RIGHT WEEK 58 C93132 M 105 APPEARANCE HUNCHED THIN EXCRETION FEW FECES SKIN & PELAGE SORE/SCAB PAW-HIND-LEFT NORMAL NO REMARKABLE OBSERVATIONS DOSE: 20 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 631, 638, 645, 652 470C, 477C, 484C, 491C, 498C, 505C, 512C, 519C, 526C, 533C, 540C 54 7C, 554C, 561C, 568C, 575C, 582C, 58 9C, 596C, 603C, 610C, 617C 624C, 631C, 638C, 645C, 652C, 659C, 666C 617C, 624C, 631C, 638C, 645C, 652C, 659C 400C, 407C, 414C, 421C, 428C, 435C, 442C, 44 9C, 456C, 463C, 470C 477C, 484C, 491C, 498C, 505C, 512C, 519C, 526C, 533C, 54 0C, 547C 554C, 561C, 568C, 575C, 582C, 58 9C, 596C, 603C, 610C, 617C, 624C 631C, 638C, 645C, 652C, 659C, 666C 729 729 729 617, 624, 631,. 638, 645, 652, 659, 666 , 673, 680, 687, 694 , 701 708, 715, 722,. 729 1 , 8 ,r 15, 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 1147 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 603 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F5 (CONTINUED FROM PREVIOUS PAGE) C93132 M 105 NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) INGUINAL REGION-RIGHT WEEK 72 VENTRAL-ABDOMINAL WEEK 97 VENTRAL-ABDOMINAL-RIGHT WEEK 58 T 105 APPEARANCE MALOCCLUSION NORMAL NO REMARKABLE OBSERVATIONS DOSE: 20 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 386, 393, 428, 435 , 442, 449, 456, 463, 470, 477 , 484, 491 , 519 526, 533, 540, 547 , 554, 561, 568, 575, 582, 589 , 596, 603 , 610 498, 505, 512 673C, 680C , 687C, 694C, 701C, 708C , 715C , 722C, 72 9C 400C, 407C , 414C, 421C 673 1 , 8 , 15, 2 2 , 29, 36, 43 , 50, 57, 64, 71 , 78, 85 , 92, 99, 106 113, 1 2 0, 127, 134 , 141, 148, 155, 162, 169, 176 , 183, 190 , 197 204, 2 1 1 , 218, 225 , 232, 239, 246, 253, 260, 267 , 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344, 351, 358 , 365, 372 , 379 386, 393, 400, 407 , 414, 421, 428, 435, 442, 449 , 456, 463 , 470 477, 484, 491, 498 , 505, 512, 519, 526, 533, 540 , 547, 554 , 561 568, 575, 582, 589 , 596, 603, 610, 617, 624, 631 , 638, 645 , 652 659, 666, 680, 687 , 694, 701, 708, 715, 722, 729 , 733 1148 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 604 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F5 DOSE: 20 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C93134 D 68 APPEARANCE MALOCCLUSION SKIN & PELAGE SORE/SCAB VENTRAL-ABDOMINAL NORMAL NO REMARKABLE OBSERVATIONS C93135 T MASS(ES) SHOULDER-LEFT WEEK 48 APPEARANCE MALOCCLUSION MISSING TEETH THIN 78, 85, 92, 99, 106 190, 197, 204, 211 1 , 8 , 15, 2 2 , 29, 36, 43 , 50, 57, 64, 71 , 113 , 120, 127, 134 141, 148, 155, 162 , 169, 176, 183, 218, 225, 232 , 239,. 246, 253 260, 267, 274, 281 , 288, 295, 302, 309, 316, 323 330C, 337C , 344C, 351C, 358C, 365C , 372C , 379C, 386C, 393C, 400C 407C, 414C , 421C, 428C, 435C, 442C , 449C , 456C, 463C, 470C, 473C 169, 260, 351, 442, 533, 624, 715, 176, 267, 358, 449, 540, 631, 722, 183, 274, 365, 456, 547, 638, 729, 190 , 197, 281 , 288, 372 , 379, 463 , 470, 554 , 561, 645 , 652, 731 204, 295, 386, 477, 568, 659, 2 1 1 , 218, 302, 309, 393, 400, 484, 491, 575, 582, 666, 673, 225, 316, 407, 498, 589, 680, 232 , 239,. 246, 323 , 330, 337, 414 , 421,. 428, 505 , 512, 519, 596 , 603,. 610, 687 , 694, 701, 253 344 435 526 617 708 512, 519, 526, 533 , 540, 547, 554, 561, 568, 575 , 582, 589, 596 603, 610, 617, 624 , 631, 638, 645, 652, 659, 666 , 673, 680, 687 694, 701, 708, 715 , 722, 729, 731 554, 561, 568, 575 1149 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 605 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F5 (CONTINUED FROM PREVIOUS PAGE) C93135 T 105 SKIN & PELAGE SORE/SCAB PAWS-HIND NORMAL NO REMARKABLE OBSERVATIONS C93136 D MASS(ES) INGUINAL REGION-LEFT WEEK 74 VENTRAL-ABDOMINAL-LEFT WEEK 74 55 NORMAL NO REMARKABLE OBSERVATIONS C93137 T APPEARANCE CONVULSIONS MALOCCLUSION MISSING TEETH SKIN & PELAGE SORE/SCAB HEAD-ENTIRE PAW-HIND-LEFT DOSE: 20 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 708, 715, 722, 729, 731 1 , 8 , 15, 2 2 , 29, 36, 43,, 50, 57, 64, 71, 78, 85, 92 113, 1 2 0, 127,, 134, 141, 148, 155, 162 512 519, 526C, 533C 1 , 8 ,r 15, 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 385 295, 302, 316,. 323, 428, 456, 477, 641 , 708 568, 575, 582, 589, 596, 603, 610, 617 , 624, 631, 638, 641 , 645 652, 659, 666,. 673, 680, 687, 694, 701 , 708, 715, 722, 729 , 732 708, 722, 729, 732 659, 666, 673, 680, 687, 694, 701, 708 617, 624, 631,. 638, 641, 645, 652, 659 , 666 1150 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 606 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F5 (CONTINUED FROM PREVIOUS PAGE) C93137 T 105 SKIN & PELAGE SORE/SCAB PAWS-HIND NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) AXILLARY REGION-LEFT WEEK 85 VENTRAL-ABDOMINAL-LEFT WEEK 77 3 53 NORMAL NO REMARKABLE OBSERVATIONS T NORMAL NO REMARKABLE OBSERVATIONS DOSE: 20 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 673, 680, 687,. 694, 701, 708, 715, 722, 729, 732 1 , 8 , 15, 2 2 , 29, 36, 43 , 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0, 127,. 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 2 1 1 , 218,. 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 309, 330, 337,. 344, 351, 358, 365, 372, 379, 386, 393, 400, 407 414, 421, 435,. 442, 449, 463, 470, 484, 491, 498, 505, 512, 519 526 58 9C, 596C , 603C, 610C, 617C, 624C 533C, 540C', 54 7C, 554C, 561C, 568C , 575C 1 , 8 , 15, 2 2 , 29, 36, 43 , 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0, 127,. 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 2 1 1 , 218,. 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309,. 316, 323, 330, 337, 344, 351, 358, 365- 366 1 , 8 , 15, 2 2 , 29, 36, 43 , 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0, 127,. 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 2 1 1 , 218,. 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309,. 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400,. 407, 414, 421, 428, 435, 442, 449, 456, 463, 477 484, 491, 498,. 505, 512, 519, 526, 533, 540, 547, 554, 561, 568 575, 582, 589,. 596, 603, 610, 617, 624, 631, 638, 645, 652, 659 1151 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 607 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F5 (CONTINUED FROM PREVIOUS PAGE) C93139 T 105 NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) VENTRAL-ABDOMINAL WEEK 68 C93140 T 105 EYES RED DISCHARGE EYE-RIGHT NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) AXILLARY REGION-RIGHT WEEK 102 INGUINAL REGION-RIGHT WEEK 104 VENTRAL-ABDOMINAL-RIGHT WEEK 93 DOSE: 20 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 666, 673, 680, 687, 694, 701, 708, 715, 722 729, 733 470C 400, 407, 414, 421 , 428, 435, 442, 449 , 456, 463, 470, 477 , 484 491, 498, 505, 512 , 519, 526, 533, 540 , 547, 554, 561, 568 , 575 582, 589, 596, 603 , 610, 617, 624, 631 , 638, 645, 652, 659 , 666 673, 680, 687,. 694 , 701, 708, 715, 722 , 729, 732 1 , 8 ,r 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393 708C, 715C, 722C, 729C, 732C 722C, 72 9C, 732C 645C, 652C, 659C, 666C, 673C, 680C, 687C, 694C, 701C, 708C, 715C 722C, 729C, 732C 1152 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 608 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F5 DOSE: 20 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C93141 M 100 BEHAVIOR HYPOACTIVE <UNSPECIFIED> EXCRETION NO FECES EYES RED DISCHARGE EYE-RIGHT SKIN & PELAGE RED HAIR COAT PERINEAL AREA NORMAL NO REMARKABLE OBSERVATIONS 698 698 698 694, 698 1, 8 , 15 113, 120 204, 211 295, 302 386, 393 477, 484 568, 575 659, 666 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 400,. 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 491,. 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 582,. 589, 596, 603, 610, 617 , 624, 631, 638, 645 , 652 673, 680, 687 1153 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 609 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F5 DOSE: 20 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C93142 1 C93143 2 C93144 1 C93145 D 4 NORMAL NO REMARKABLE OBSERVATIONS 14 NORMAL NO REMARKABLE OBSERVATIONS 4 NORMAL NO REMARKABLE OBSERVATIONS 75 NORMAL NO REMARKABLE OBSERVATIONS C93146 T MASS(ES) AXILLARY REGION-RIGHT WEEK 72 105 APPEARANCE THIN NORMAL NO REMARKABLE OBSERVATIONS 1, 8 , 15, 22 1 , 8 , 15, 22, 29, 36, 43, 50, 57, 64, 71, 78 1 , 8 , 15, 22 1 , 8 15, 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 519, 523 498, 505, 512 554, 561 1 , 8 ,r 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0, 127 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400 407 , 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491 498 , 505, 512, 519, 526 , 533, 540, 547, 568 , 575 582, 589, 596 603 , 610, 617, 624, 631 , 638, 645, 652, 659 , 666 1154 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 610 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F5 (CONTINUED FROM PREVIOUS PAGE) C93146 T 105 NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) AXILLARY REGION-RIGHT WEEK 100 WEEK 102 VENTRAL-ABDOMINAL-LEFT WEEK 100 C93147 3 53 NORMAL NO REMARKABLE OBSERVATIONS C93148 D 91 EYES CLEAR DISCHARGE EYE-RIGHT NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 79 DOSE: 20 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 673, 680, 687 694 708C, 715C, 722C, 729C, 732C 694C, 701C, 708C, 715C, 722C, 729C, 732C 1 , 8 ,r 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190, 197 204, 2 1 1 , 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281, 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365-366 547, 554, 561 1 , 8 ,r 15, 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 589, 596 , 603 610, 617, 624, 631, 636 547, 554, 561,. 568, 575, 582 1155 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 611 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F5 DOSE: 20 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C93149 T 105 EXCRETION FEW FECES EYES PROTRUDING EYES SKIN & PELAGE ALOPECIA RED HAIR COAT HEAD-ENTIRE NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) AXILLARY REGION-LEFT WEEK 91 HEAD-MAXILLARY-LEFT WEEK 98 VENTRAL-CERVICAL-LEFT WEEK 100 701, 708, 715,722 512 554 547, 554 1 , 8 ,r 15, 2 2 , 29, 36, 43,r 50, 113, 1 2 0, 127, 134 , 141, 148, 204, 2 1 1 , 218, 225 , 232, 239, 295, 302, 309, 316 , 323, 330, 386, 393, 400, 407 , 414, 421, 477, 484, 491, 498 , 505, 519, 5c 8o 9o , 5c 9o 6tz, 6 T0O3O , 6tZ 11 0o , 6tz 1i 7n , t6z 20/41 57, 64, 71, 78, 85, 155, 162 , 169, 176, 246, 253 , 260, 267, 337, 344 , 351, 358, 428, 435 , 442, 449, 526, 533 , 540, 561, 92, 99, 106 183, 190 , 197 274, 281 , 288 365, 372 , 379 456, 463 , 470 568, 575 , 582 631C, 638C, 645C, 652C, 659C, 666C, 673C, 680C, 687C, 694C, 701C 708C, 715C, 722C, 729C, 731C 680 694C, 701C, 708C, 715C, 722C, 729C, 731C 1156 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 612 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F5 DOSE: 20 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C93150 M 92 APPEARANCE HEAD TILT RIGHT THIN BEHAVIOR RECUMBENT LATERAL DISCHARGE NASAL RED IN COLOR EXCRETION FEW FECES EYES SQUINTED PERI-ORBITAL-RIGHT RESPIRATION IRREGULAR NORMAL NO REMARKABLE OBSERVATIONS 610, 617, 624 624, 631 640 640 624, 631 547, 554 640 1 22, 8,r 15, , 29, 36, 43,r 50, 57, 64, 71, 78, 85 120113, , 127, 134 , 141, 148, 155, 162 , 169, 176 211204, , 218, 225 , 232, 239, 246, 253 , 260, 267 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358 386, 393, 400, 407 , 414, 421, 428, 435 , 442, 449 477, 484, 491, 498 , 505, 512, 519, 526 , 533, 540 603 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 582, 589, 596 1157 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 613 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F5 (CONTINUED FROM PREVIOUS PAGE) C93150 M 92 MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 81 C93151 T 105 DISCHARGE GENITAL RED IN COLOR NORMAL NO REMARKABLE OBSERVATIONS ^SS(ES) AXILLARY REGION-LEFT WEEK 89 INGUINAL REGION-LEFT WEEK 79 DOSE: 20 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 561, 568, 575 610 1 , 8 ,. 15, 2 2 , 2 9, 36, 43 , 50 , 57, 64, 71 , 78,, 85, 92, 99, 106 113, 1 2 0, 127, 134, 141, 148 , 155, 162, 169, 176, 183,. 190, 197 204, 2 1 1 , 218, 225, 232, 239 , 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330 , 337, 344, 351, 358, 365,. 372, 379 386, 393, 400, 407, 414, 421 , 428, 435, 442, 449, 456,. 463, 470 477, 484, 491, 498, 505, 512 , 519, 526, 533, 540 617, 624, 631C, 638C, 645C, 652C, 659C, 666C, 673C, 680C, 687C 694C, 701C , 708C, 715C, 722C , 72 9C , 733C 547, 554, 561C, 568C, 575C, 582C, 58 9C, 596C, 603C, 610C, 617C 624C, 631C , 638C, 645C, 652C , 659C , 666C , 673C, 680C, 687C, 694C 701C, 708C , 715C, 722C, 72 9C , 733C 1158 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 614 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F5 DOSE: 20 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C93152 M 66 APPEARANCE HUNCHED THIN BEHAVIOR ATAXIC <UNSPECIFIED> DISCHARGE NASAL RED IN COLOR EXCRETION FEW FECES EYES RED DISCHARGE EYES NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) AXILLARY REGION-RIGHT WEEK 53 449, 456 456 456 456 442, 449 442, 456 1 , 8 ,r 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0, 127, 134 , 141, 148, 155, 162 , 169, 176, 183,. 190, 197 204, 2 1 1 , 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281, 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358 365C, 372C, 379C, 386C, 393C, 400C, 407C, 414C, 421C, 428C, 435C 442C, 449C, 456C 1159 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 615 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F5 DOSE: 20 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C93153 T 105 APPEARANCE SWOLLEN PAW-HIND-LEFT SKIN & PELAGE SORE/SCAB PAW-HIND-LEFT PAWS-HIND NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) DORSAL WEEK 91 VENTRAL-ABDOMINAL-RIGHT WEEK 81 659, 666, 673, 680 , 687, 694, 701, 708, 715, 722 , 729, 732 505, 547, 554, 561 , 568, 575, 582, 589, 596, 603 , 610, 617 , 624 631, 638, 645, 652 , 659, 666, 673, 680, 687, 694 , 701 708, 715, 722,. 729 , 732 1 , 8 , 15, 2 2 , 29, 36, 43 , 50, 57, 64, 71 , 78, 85 , 92, 99, 106 113, 1 2 0, 127, 134 , 141, 148, 155, 162, 169, 176 , 183, 190 , 197 204, 2 1 1 , 218, 225 , 232, 239, 246, 253, 260, 267 , 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344, 351, 358 , 365, 372 , 379 386, 393, 400, 407 , 414, 421, 428, 435, 442, 449 , 456, 463 , 470 477, 484, 491, 498 , 512, 519, 526, 533, 540 631C, 638C , 645C, 652C, 659C, 666C , 673C , 680C, 687C, 694C , 701 708C, 715C , 722C, 729C, 732C 561, 568, 575, 582 1160 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 616 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F5 DOSE: 20 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C93154 3 53 NORMAL NO REMARKABLE OBSERVATIONS C93155 2 C93156 M SURGERY PERFORMED BY JOHN VAN BERKUM ANTIBIOTIC ADMINISTERED 0.5 ML 14 NORMAL NO REMARKABLE OBSERVATIONS 86 APPEARANCE THIN EXCRETION FEW FECES EYES CLEAR DISCHARGE EYE-LEFT EYE-RIGHT EYES PALE EYE-RIGHT PROTRUDING EYE-RIGHT 1 , 8 , 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0, 127, 134, 141, 148, 155,. 162, 169, 176, 183, 190, 197 204, 2 1 1 , 218, 225, 232, 239, 246,. 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337,. 344, 351, 358, 365-366 359 359- 360 1 , 8 , 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92-93 533, 540, 547, 554, 561, 568, 575,. 582, 589, 595- 596 531, 533, 540 561 568, 575, 582, 589 547, 554 595-596 595-596 1161 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 617 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F5 (CONTINUED FROM PREVIOUS PAGE) C93156 M 86 EYES RED DISCHARGE EYE-RIGHT EYES SCAB EYE-RIGHT SKIN & PELAGE SORE/SCAB VENTRAL-ABDOMINAL VENTRAL-CERVICAL NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) VENTRAL-ABDOMINAL WEEK 84 VENTRAL-CERVICAL WEEK 53 DOSE: 20 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 442, 449, 456, 463, 470, 561 491, 498, 505, 512, 519, 526, 531, 533, 540 595- 596 435 568, 575, 582, 589, 595-596 1 , 8 , 15, 2 2 , 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253, 260, 267, 274, 281 , 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358 582C , 589C , 595-596C 365C , 372C , 379C, 386C, 393C, 400C , 407C, 414C, 421C, 428C , 435C 442C , 449C , 456C, 463C, 470C, 477C , 484C, 491C, 498C, 505C , 512C 519C , 526C , 531C, 533C, 540C, 547C , 554C, 561C, 568C, 575C , 582C 589C , 595- 596C 1162 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 618 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F5 DOSE: 20 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C93157 T 105 EYES PROTRUDING EYES NORMAL NO REMARKABLE OBSERVATIONS WART LIKE LESION(S) MOUTH WEEK 47 T 105 NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) INGUINAL REGION-RIGHT WEEK 71 VENTRAL-ABDOMINAL-RIGHT WEEK 71 512 1 , 8 ,. 15, 2 2 , 29, 36, 43 , 50, 57, 64, 71, 78, 85 , 92, 99, 106 113, 1 2 0, 127,. 134, 141, 148, 155, 162, 169, 176 , 183, 190 , 197 204, 2 1 1 , 218,. 225, 232, 239, 246, 253, 260, 267 , 274, 281 , 288 295, 302, 309,. 316, 435, 442, 449, 456, 463, 470 , 477, 484 , 491 498, 505, 519,. 526, 533, 540, 547, 554, 561, 568 , 575, 582 , 589 596, 603, 610,. 617, 624, 631, 638, 645, 652, 659 , 666, 673 , 680 687, 694, 701,. 708, 715, 722, 729, 733 323, 330, 337,. 344, 351, 358, 365, 372, 379, 386 , 393, 400 , 407 414, 421, 428 1 , 8 ,. 15, 2 2 , 29, 36, 43 , 50, 57, 64, 71, 78, 85 , 92, 99, 106 113, 1 2 0, 127,. 134, 141, 148, 155, 162, 169, 176 , 183, 190 , 197 204, 2 1 1 , 218,. 225, 232, 239, 246, 253, 260, 267 , 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344, 351, 358 , 365, 372 , 379 386, 393, 400,. 407, 414, 421, 428, 435, 442, 449 , 456, 463 , 470 477, 484 491C, 498C , 505C, 512C 519C, 526C', 53 3C, 54 0C, 54 7C, 554C , 561C, 568C, 575C, 582C , 589C 596C, 603C , 610C, 617C, 624C, 631C , 638C, 645C, 652C, 659C , 666C 1163 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 619 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F5 (CONTINUED FROM PREVIOUS PAGE) C93158 T 105 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 71 C93159 1 4 NORMAL NO REMARKABLE OBSERVATIONS C93160 M 76 APPEARANCE SWOLLEN VENTRAL-ABDOMINAL THIN BEHAVIOR COLD TO TOUCH ENTIRE BODY EXCRETION FEW FECES NO FECES EYES SQUINTED PERI-ORBITAL-RIGHT SKIN & PELAGE BROWN HAIR COAT PERINEAL AREA SORE/SCAB HEAD-MAXILLARY-RIGHT PAWS-HIND YELLOW HAIR COAT DOSE: 20 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 673C, 680C, 687C, 694C, 701C, 708C, 715C, 722C, 729C, 731C 1, 8 , 15, 22 519, 526 519, 526 526 491, 498, 504-505, 512, 526 506 504 505-506, 512 197, 204, 211 505-506, 512, 519, 526 519, 526 1164 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 620 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F5 (CONTINUED FROM PREVIOUS PAGE) C93160 M 76 NORMAL NO REMARKABLE OBSERVATIONS C93161 T WART LIKE LESION(S) NOSE WEEK 72 105 APPEARANCE SWOLLEN DORSAL LIMB-HIND-LEFT PAW-HIND-LEFT SKIN & PELAGE SORE/SCAB PAW-HIND-LEFT NORMAL NO REMARKABLE OBSERVATIONS DOSE: 20 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 1, 8 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 218 225, 232, 239, 246 , 253, 260, 267, 274 , 281, 288, 295, 302 , 309 316, 323, 330, 337 , 344, 351, 358, 365 , 372, 379, 386, 393 , 400 407, 414, 421, 428 , 435, 442, 449, 456 , 463, 470, 477, 484 498, 504-506, 512, 519, 526 589 589, 596, 603, 610 589, 596, 603 729, 731 1 , 8 ,r 15, 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582, 617, 624, 631, 638, 645 , 652, 659, 666, 673 , 680 687, 694, 701,. 708, 715, 722 1165 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 621 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F5 DOSE: 20 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C93162 T 105 APPEARANCE MALOCCLUSION MISSING TEETH NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) INGUINAL REGION-LEFT WEEK 102 VENTRAL-ABDOMINAL WEEK 93 90, 106, 113, 1 2 0, 127, 134, 155, 162, 169, 176, 183, 190, 197 204, 2 1 1 ,. 218, 225 , 232, 239,. 246, 253 , 260, 267,. 274, 281, 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365,. 372, 379 386, 393, 400, 407 , 414, 421,. 428, 435 , 442, 449,. 456,. 463, 470 477, 484, 491, 498 , 505, 512, 519, 526 , 533, 540, 547,. 554, 561 568, 575,. 582, 589 , 596, 603,. 610, 617 , 624, 631,. 638, 645, 652 659, 666,. 673, 680 , 687, 694, 701, 708 , 715, 722,. 729, 731 90, 113, 1 2 0, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190 197, 204, 2 1 1 , 218 , 225, 232, 239, 246 , 253, 260, 267,. 274, 281 288, 295, 302, 309 , 316, 323, 330, 337 , 344, 351,. 358, 365, 372 379, 386, 393, 400 , 407, 414, 421, 428 , 435, 442, 449,. 456, 463 470, 477,. 484, 491 , 498, 505,. 512, 519 , 526, 533,. 540, 547, 554 561, 568,. 575, 582 , 589, 596, 603, 610 , 617, 624, 631,. 638, 645 652, 659,. 666, 673 , 680, 687,. 694, 701 , 708, 715,. 722,. 729, 731 1 , 8 , 15,. 2 2 , 29, 36, 43 , 50,. 57, 64, 71, 78 , 85,. 92, 99 708C, 715C, 722C, 729C, 731C 645, 652, 659 1166 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 622 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F5 DOSE: 20 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C93163 3 53 NORMAL NO REMARKABLE OBSERVATIONS SURGERY PERFORMED BY PATTIE MURPHY ANTIBIOTIC ADMINISTERED 0.5 ML T SKIN & PELAGE BLUE SKIN VENTRAL-ABDOMINAL-LEFT NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) INGUINAL REGION-LEFT WEEK 77 1 , 8 ,r 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 113, 1 2 0, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 204, 2 1 1 , 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365-366 197 288 359 359-360 729, 731 1 , 8 ,. 15, 22, 29, 36, 43 , 50, 57, 64, 71, 78, 85 , 92, 99, 106 113, 1 2 0, 127, 134, 141, 148, 155, 162, 169, 176 , 183, 190 , 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253, 260, 267 , 274, 281 , 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358 , 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435, 442, 449 , 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 533C, 540C , 547C, 554C, 561C, 568C , 575C, 582C, 58 9C, 596C , 603C 610C, 617C , 624C, 631C, 638C, 645C , 652C, 659C, 666C, 673C , 680C 687C, 694C , 701C, 708C, 715C, 722C , 729C, 731C 1167 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 623 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F5 DOSE: 20 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C93165 T 105 SKIN & PELAGE ALOPECIA SORE/SCAB DORSAL-CERVICAL DORSAL-CERVICAL-LEFT DORSAL-CERVICAL-RIGHT NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) AXILLARY REGION-RIGHT WEEK 78 VENTRAL-THORACIC WEEK 93 C93166 T 105 SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE NORMAL NO REMARKABLE OBSERVATIONS 554, 561, 666 ,. 673, 680 708, 715, 722,. 729, 733 666, 673, 708,. 715, 722, 729, 733 729, 733 1 , 8 ,r 15, 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533 54 0C, 54 7C, 554C, 561C, 568C, 575C, 582C, 58 9C, 596C, 603C, 610C 617C, 624C, 631C, 638C, 645C, 652C 645C, 652C, 659C, 666C, 673C, 680C, 687C, 694C, 701C, 708C, 715C 722C, 729C, 733C 512, 519, 526 1 , 8 ,r 15, 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 1168 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 624 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F5 (CONTINUED FROM PREVIOUS PAGE) C93166 T NORMAL NO REMARKABLE OBSERVATIONS C93167 M 39 APPEARANCE MALOCCLUSION MISSING TEETH THIN EXCRETION FEW FECES SKIN & PELAGE PALE ENTIRE BODY ROUGH HAIR COAT YELLOW HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS DOSE: 20 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470 477, 484, 491, 498, 505, 533, 540, 547, 554, 561, 568, 575, 582 589, 596, 603, 610, 617, 624, 631, 638, 645, 652, 659, 666, 673 680, 687, 694, 701, 708, 715, 722, 729, 732 253, 260, 267-268 253 268 260, 267-268 267- 268 260, 267-268 260, 267-268 1 , 8 , 15, 2 2 , 29, 36, 43,, 50, 57, 64, 71, 78, 85 92, 99, 106 113, 1 2 0, 127,, 134, 141, 148, 155, 162 , 169, 176 183, 190, 197 204, 2 1 1 , 218,, 225, 232, 239, 246 1169 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 625 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F5 DOSE: 20 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C93168 T .05 NORMAL NO REMARKABLE OBSERVATIONS C93169 2 C93170 M 14 NORMAL NO REMARKABLE OBSERVATIONS 94 APPEARANCE HEAD TILT RIGHT BEHAVIOR ATAXIC <UNSPECIFIED> EYES RED DISCHARGE EYE-LEFT NORMAL NO REMARKABLE OBSERVATIONS 1 22, 8,r 15, , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 120113, , 127,. 134, 141, 148, 155, 162 , 169, 176, 183 , 190 , 197 211204, , 218, 225, 232, 239, 246, 253 , 260, 267, 274 , 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365 , 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456 , 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547 , 554 , 561 666568, 575, 582, 589, 596, 603, 610, 617 , 624, 631, 638 , 645 , 652 659, , 673, 680, 687, 694, 701, 708 , 715, 722, 729 , 732 1 22, 8,r 15, , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92- 93 645, 652-653 653 645, 652-653 1 22, 8,r 15, , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 120113, , 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 211204, , 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407 , 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498 , 505, 512, 519, 526 , 533, 540, 547, 554 , 561 1170 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 626 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F5 (CONTINUED FROM PREVIOUS PAGE) C93170 M 94 NORMAL NO REMARKABLE OBSERVATIONS C93171 3 53 NORMAL NO REMARKABLE OBSERVATIONS SURGERY PERFORMED BY JOHN VAN BERKUM ANTIBIOTIC ADMINISTERED 0.5 ML M 78 APPEARANCE HEAD TILT LEFT HUNCHED THIN BEHAVIOR ATAXIC <UNSPECIFIED> HYPOACTIVE <UNSPECIFIED> EXCRETION FEW FECES DOSE: 20 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 568, 575, 582, 589, 596, 603, 610, 617 , 624, 631, 638 1 , 8 ,r 15, 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190, 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281, 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365-366 359 519, 526, 528, 533, 540, 546 540, 546 533, 540, 546 546 546 504-505, 512, 528, 533, 540, 546 1171 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 627 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F5 (CONTINUED FROM PREVIOUS PAGE) C93172 M 78 EYES RED DISCHARGE EYE-RIGHT SQUINTED PERI-ORBITAL-RIGHT SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE SORE/SCAB PAW-HIND-RIGHT NORMAL NO REMARKABLE OBSERVATIONS C93173 M 102 DISCHARGE NASAL RED IN COLOR EXCRETION FEW FECES EYES RED DISCHARGE EYES DOSE: 20 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 546 498, 504-505, 512, 519, 526, 528, 533, 540, 546 505, 512, 519 512 1, 8 , 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 2 2 , 29, 36, 43,r 50, 127, 134 , 141, 148, 218, 225 , 232, 239, 309, 316 , 323, 330, 400, 407 , 414, 421, 491 57, 64, 71, 78, 85, 155, 162 , 169, 176, 246, 253 , 260, 267, 337, 344 , 351, 358, 428, 435 , 442, 449, 92, 99, 106 183, 190 , 197 274, 281 , 288 365, 372 , 379 456, 463 , 470 705, 708-709 705, 708-709 705, 708-709 1172 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 628 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F5 (CONTINUED FROM PREVIOUS PAGE) C93173 M 102 NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) AXILLARY REGION-LEFT WEEK 81 SHOULDER-RIGHT WEEK 88 C93174 T 105 APPEARANCE MALOCCLUSION NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) VENTRAL-THORACIC WEEK 100 DOSE: 20 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 1 , 8 ,r 15, 2 2 , 29, 36 , 43,r 50, 113, 1 2 0 , 127,. 134, 141, 148, 204, 2 1 1 , 218, 225, 232, 239, 295, 302, 309,. 316, 323, 330, 386, 393, 400, 407, 414, 421, 477, 484, 491, 498, 505, 512, 57, 64, 71, 78, 85, 155, 162 , 169, 176, 246, 253 , 260, 267, 337, 344 , 351, 358, 428, 435 , 442, 449, 519, 526 , 533, 540, 92, 99, 106 183, 190 , 197 274, 281 , 288 365, 372 , 379 456, 463 , 470 547, 554 561, 568, 575, 582, 589, 596, 603 610C, 617C, 624C, 631C, 638C, 645C, 652C, 659C, 666C, 673C, 680C 687C, 694C, 701C, 705C, 708-709C 400, 407, 414, 421 , 428, 435, 442, 449 , 456, 463, 470, 477 , 484 491, 498, 505, 512 , 519, 526, 533, 540 , 547, 554, 561, 568 , 575 582, 589, 596, 603 , 610, 617, 624, 631 , 638, 645, 652, 659 , 666 673, 680, 687,. 694 , 701, 708, 715, 722 , 729, 731 1 , 8 , 15, 2 2 , 29, 36, 43 , 50, 113, 1 2 0 , 127, 134 , 141, 148, 204, 2 1 1 , 218, 225 , 232, 239, 295, 302, 309, 316 , 323, 330, 386, 393 57, 64, 71, 78, 85, 155, 162 , 169, 176, 246, 253 , 260, 267, 337, 344 , 351, 358, 92, 99, 106 183, 190 , 197 274, 281 , 288 365, 372 , 379 694C, 701C , 708C, 715C, 722C, 72 9C , 73 1C 1173 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 629 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F5 DOSE: 20 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C93175 M 92 APPEARANCE THIN TREMORS ENTIRE BODY BEHAVIOR HYPOACTIVE <UNSPECIFIED> COLD TO TOUCH ENTIRE BODY EXCRETION FEW FECES NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 75 638, 641 641 641 641 491, 638, 641 1 , 8 , 15, 2 2 , 29, 36, 43 , 50, 57, 64, 71, 78, 85 , 92, 99, 106 113, 1 2 0 , 127,. 134, 141, 148, 155, 162, 169, 176 , 183, 190 , 197 204, 2 1 1 , 218,. 225, 232, 239, 246, 253, 260, 267 , 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344, 351, 358 , 365, 372 , 379 386, 393, 400,. 407, 414, 421, 428, 435, 442, 449 , 456, 463 , 470 477, 484, 498, 505, 512 519C, 526C , 533C, 540C, 54 7C, 554C , 561C, 568C, 575C, 582C , 589C 596C, 603C , 610C, 617C, 624C, 631C , 638C, 641C 1174 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 630 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F5 DOSE: 20 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C93176 M 88 APPEARANCE HUNCHED THIN BEHAVIOR HYPOACTIVE <UNSPECIFIED> RECUMBENT LATERAL EXCRETION NO FECES EYES PROTRUDING EYES RED DISCHARGE EYES SKIN & PELAGE BLACK SKIN VENTRAL-ABDOMINAL-LEFT ROUGH HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS 610, 613 610, 613 610, 613 613 613 512 610, 613 596, 603, 610, 613 610, 613 1 , 8 ,r 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 400 428, 435, 442, 449 , 456, 463, 470, 477 , 484, 491 1175 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 631 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F5 (CONTINUED FROM PREVIOUS PAGE) C93176 M 88 MASS(ES) AXILLARY REGION-LEFT WEEK 59 AXILLARY REGION-RIGHT WEEK 55 INGUINAL REGION-LEFT WEEK 72 INGUINAL REGION-RIGHT WEEK 74 WEEK 76 WEEK 79 C93177 T 105 APPEARANCE MALOCCLUSION SWOLLEN VENTRAL-ABDOMINAL DISCHARGE CLEAR ORAL <UNSPECIFIED> EYES PROTRUDING EYES SKIN & PELAGE SORE/SCAB PAW-HIND-LEFT DOSE: 20 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 407, 414, 421 379, 386, 393 498C, 505C, 512C, 519C, 526C, 533C,. 540C, 547C, 554C, 561C, 568C 575C,. 582C, 589C, 596C, 603C, 610C, 613C 512 526, 533 54 7C, 554C, 561C, 568C, 575C, 582C,. 589C, 596C, 603C, 610C, 613C 421, 428, 435, 442, 449,, 456, 463, 470, 477, 484, 491, 498, 505 512, 519, 526, 533, 540, 547, 554, 561, 568, 575, 582, 589, 596 603, 610, 617, 624, 631,, 638, 645, 652, 659, 666, 673, 680, 687 694, 701, 708, 715, 722,, 729, 733 554 568, 575 491, 498, 505, 512 708, 715 1176 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 632 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F5 (CONTINUED FROM PREVIOUS PAGE) C93177 T 105 NORMAL NO REMARKABLE OBSERVATIONS T 105 EYES RED DISCHARGE EYE-LEFT NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) HEAD-MAXILLARY-LEFT WEEK 67 DOSE: 20 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 1 , 8 ,r 15, 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 10 6 113, 1 2 0 , 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190, 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281, 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372, 379 386, 393, 400, 407, 414 708, 715, 722,. 729, 733 1 , 8 ,r 15, 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 10 6 113, 1 2 0 , 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190, 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281, 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 470, 477 484, 491, 498, 505, 512, 519, 526, 533 , 540, 547, 554, 561, 568 575, 582, 589,. 596, 603, 610, 617, 624 , 631, 638, 645, 652, 659 666, 673, 680, 687, 694, 701 463 1177 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 633 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F5 DOSE: 20 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C93179 T 105 EXCRETION FEW FECES SKIN & PELAGE ALOPECIA SORE/SCAB AXILLARY REGION-LEFT NORMAL NO REMARKABLE OBSERVATIONS C93180 M MASS(ES) AXILLARY REGION-LEFT WEEK 95 63 SKIN & PELAGE SORE/SCAB VENTRAL-CERVICAL-RIGHT NORMAL NO REMARKABLE OBSERVATIONS 708 554, 561, 568, 575 , 582, 589, 596, 603, 610, 617 , 624, 631 , 638 645, 652 701, 708, 715, 722 , 729, 733 1 , 8 , 15, 2 2 , 29, 36, 43 , 50, 57, 64, 71 , 78, 85 , 92, 99, 106 113, 1 2 0 , 127, 134 , 141, 148, 155, 162, 169, 176 , 183, 190 , 197 204, 2 1 1 , 218, 225 , 232, 239, 246, 253, 260, 267 , 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344, 351, 358 , 365, 372 , 379 386, 393, 400, 407 , 414, 421, 428, 435, 442, 449 , 456, 463 , 470 477, 484, 491, 498 , 505, 512, 519, 526, 533, 540 , 547 659C, 666C , 673C, 680C, 687C, 694C , 701C , 708C, 715C, 722C , 72 9C 733C 57 1 , 8 ,r 15, 2 2 , 29, 36, 43,r 50, 64, 71, 78 , 85,, 92, 99, 106, 113 1 2 0 , 127, 134, 141 , 148, 155, 162, 169, 176, 183, 190, 197, 204 2 1 1 , 218, 225, 232 , 239, 246, 253, 260, 267, 274, 281,. 288, 295 302, 309, 316, 323 , 330, 337, 344, 351, 358, 365, 372, 379 1178 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 634 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F5 (CONTINUED FROM PREVIOUS PAGE) C93180 M 63 MASS(ES) VENTRAL-THORACIC-LEFT WEEK 56 DOSE: 20 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 386C, 393C, 400C, 407C, 414C, 421C, 428C, 435C 1179 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C93113 C93118 F5 715 103 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 103 722 104 MASS(ES) TO 731 105 VENTRAL-ABDOMINAL-LEFT WEEK 103 F5 337 49 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 49 344 50 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 49 351 51 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 49 358 52 TO 365 53 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 49 372 54 TO 386 56 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 49 393 57 TO 421 61 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 49 20 X 20MM 20 X 20MM 10 X 15MM 20 X 20MM 30 X 25MM 25 X 20 30 X 25MM 30 X 30MM 1180 Covance 6329-183 3M T-6295 PAGE: 635 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT F5 428 62 435 63 442 64 449 65 TO 477 69 484 70 TO 491 71 498 72 505 73 512 74 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 49 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 49 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 49 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 49 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 49 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 49 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 49 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 49 40 X 30MM 30 X 30MM 40 X 60MM VENTRAL HIND MASSES MERGED TOGETHER 60 X 70MM VENTRAL HIND MASSES MERGED TOGETHER 60 X 90MM 70 X 90MM 70 X 70MM 70 X 90MM 1181 Covance 6329-183 3M T-6295 PAGE: 636 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 75 MASS(ES) 76 VENTRAL-ABDOMINAL-LEFT WEEK 49 77 MASS(ES) 79 VENTRAL-ABDOMINAL-LEFT WEEK 49 80 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 49 81 MASS(ES) 84 VENTRAL-ABDOMINAL-LEFT WEEK 49 85 MASS(ES) 90 VENTRAL-ABDOMINAL-LEFT WEEK 49 91 MASS(ES) 93 VENTRAL-ABDOMINAL-LEFT WEEK 49 53 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 53 54 MASS(ES) 56 VENTRAL-ABDOMINAL-RIGHT WEEK 53 60 X 75MM 60 X 90MM 70 X 100MM 70 X 85MM 80 X 85MM 90 X 90MM 10 X 10MM 15 X 15MM 1182 Covance 6329-183 3M T-6295 PAGE: 637 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C93118 C93122 F5 393 57 TO 428 62 435 63 F5 512 74 498 72 TO 533 77 540 78 TO 575 83 582 84 TO 642 92 540 78 TO 568 82 575 83 TO 642 92 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 53 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 53 MASS(ES) INGUINAL REGION-LEFT WEEK 71 MASS(ES) INGUINAL REGION-RIGHT WEEK 72 MASS(ES) INGUINAL REGION-RIGHT WEEK 72 MASS(ES) INGUINAL REGION-RIGHT WEEK 72 MASS(ES) INGUINAL REGION-RIGHT WEEK 78 MASS(ES) INGUINAL REGION-RIGHT WEEK 78 20 X 20MM 25 X 20MM 10 X 10MM 10 X 10MM 15 X 15MM 20 X 15MM 10 X 10MM ANTERIOR TO OTHER MASS 20 X 20MM ANTERIOR TO OTHER MASS 1183 Covance 6329-183 3M T-6295 PAGE: 638 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 75 MASS(ES) 78 VENTRAL-ABDOMINAL-LEFT WEEK 71 79 MASS(ES) 82 VENTRAL-ABDOMINAL-LEFT WEEK 71 83 MASS(ES) 84 VENTRAL-ABDOMINAL-LEFT WEEK 71 85 MASS(ES) 88 VENTRAL-ABDOMINAL-LEFT WEEK 71 89 MASS(ES) 92 VENTRAL-ABDOMINAL-LEFT WEEK 71 67 MASS(ES) 68 VENTRAL-ABDOMINAL-RIGHT WEEK 67 69 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 67 70 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 67 10 X 10MM 20 X 10MM 20 X 20MM 30 X 30MM 40 X 40MM 20 X 20MM 20 X 30MM 30 X 40MM 1184 Covance 6329-183 3M T-6295 PAGE: 639 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 71 MASS(ES) 74 VENTRAL-ABDOMINAL-RIGHT WEEK 67 75 MASS(ES) 76 VENTRAL-ABDOMINAL-RIGHT WEEK 67 77 MASS(ES) 82 VENTRAL-ABDOMINAL-RIGHT WEEK 67 83 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 67 84 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 67 85 MASS(ES) 87 VENTRAL-ABDOMINAL-RIGHT WEEK 67 88 MASS(ES) 92 VENTRAL-ABDOMINAL-RIGHT WEEK 67 76 MASS(ES) 89 AXILLARY REGION-LEFT WEEK 73 20 X 40MM 20 X 30MM 25 X 35MM 35 X 35MM 40 X 35MM 50 X 40MM 60 X 40MM 10 X 10MM 1185 Covance 6329-183 3M T-6295 PAGE: 640 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT F5 652 94 TO 659 95 MASS(ES) AXILLARY REGION-LEFT WEEK 94 708 102 MASS(ES) TO 732 105 AXILLARY REGION-LEFT WEEK 102 526 76 TO 554 80 MASS(ES) DORSAL WEEK 76 561 81 TO 582 84 MASS(ES) DORSAL WEEK 76 526 76 TO 554 80 MASS(ES) INGUINAL REGION-LEFT WEEK 76 561 81 TO 582 84 MASS(ES) INGUINAL REGION-LEFT WEEK 76 498 72 TO 512 74 MASS(ES) INGUINAL REGION-RIGHT WEEK 72 659 95 MASS(ES) TO 732 105 SHOULDER-LEFT WEEK 95 10 X 10MM 10 X 10MM 10 X 10MM 20 X 20MM 10 X 10MM 15 X 15MM 10 X 10MM 10 X 10MM 1186 Covance 6329-183 3M T-6295 PAGE: 641 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT F5 491 71 TO 568 82 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 71 575 83 TO 582 84 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 71 589 85 TO 603 87 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 71 610 TO 652 94 CO CO MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 71 659 95 TO 666 96 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 71 673 97 TO 687 99 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 71 694 100 MASS(ES) TO 701 101 VENTRAL-ABDOMINAL-LEFT WEEK 71 708 102 MASS(ES) TO 722 104 VENTRAL-ABDOMINAL-LEFT WEEK 71 10 X 10MM 20 X 20MM 30 X 30MM 20 X 25MM 30 X 20MM 30 X 30MM 10 X 10MM 20 X 20MM 1187 Covance 6329-183 3M T-6295 PAGE: 642 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS CATEGORY KEYWORD WK QUALIFIER FREE-TEXT COMMENT 105 MASS(ES) 105 VENTRAL-ABDOMINAL-LEFT WEEK 71 75 MASS(ES) 84 VENTRAL-ABDOMINAL-RIGHT WEEK 72 85 MASS(ES) 86 VENTRAL-ABDOMINAL-RIGHT WEEK 72 87 MASS(ES) 92 VENTRAL-ABDOMINAL-RIGHT WEEK 72 93 MASS(ES) 94 VENTRAL-ABDOMINAL-RIGHT WEEK 72 95 MASS(ES) 101 VENTRAL-ABDOMINAL-RIGHT WEEK 72 102 MASS(ES) 104 VENTRAL-ABDOMINAL-RIGHT WEEK 72 105 MASS(ES) 105 VENTRAL-ABDOMINAL-RIGHT WEEK 72 30 X 40 MM 10 X 10MM 35 X 25MM 50 X 30MM 50 X 40MM 50 X 50MM 60 X 60MM 70 X 60 MM 1188 Covance 6329-183 3M T-6295 PAGE: 643 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS CATEGORY KEYWORD WK QUALIFIER FREE-TEXT COMMENT 85 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 85 94 MASS(ES) 101 VENTRAL-ABDOMINAL-RIGHT WEEK 94 102 MASS(ES) 103 VENTRAL-ABDOMINAL-RIGHT WEEK 94 104 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 94 105 MASS(ES) 105 VENTRAL-ABDOMINAL-RIGHT WEEK 94 102 MASS(ES) 105 VENTRAL-CERVICAL-LEFT WEEK 102 71 MASS(ES) 81 VENTRAL-THORACIC-RIGHT WEEK 71 82 MASS(ES) 84 VENTRAL-THORACIC-RIGHT WEEK 71 10 X 10MM 20 X 15MM 10 X 10MM 20 X 20MM 30 X 30 MM 10 X 10MM 15 X 10MM 20 X 20MM 1189 Covance 6329-183 3M T-6295 PAGE: 644 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C93124 C93131 F5 589 85 TO 610 88 MASS(ES) VENTRAL-THORACIC-RIGHT WEEK 71 617 89 MASS(ES) VENTRAL-THORACIC-RIGHT WEEK 71 673 97 MASS(ES) TO 732 105 VENTRAL-THORACIC-RIGHT WEEK 97 F5 470 68 TO 484 70 MASS(ES) AXILLARY REGION-RIGHT WEEK 68 491 71 MASS(ES) AXILLARY REGION-RIGHT WEEK 68 498 72 TO 512 74 MASS(ES) AXILLARY REGION-RIGHT WEEK 68 519 75 MASS(ES) AXILLARY REGION-RIGHT WEEK 68 526 76 TO 554 80 MASS(ES) AXILLARY REGION-RIGHT WEEK 68 15 X 15MM 10 X 10MM 20 X 15MM 20 X 20MM 20 X 30MM 20 X 25MM 30 X 20MM 30 X 30MM 1190 Covance 6329-183 3M T-6295 PAGE: 645 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 81 MASS(ES) 84 AXILLARY REGION-RIGHT WEEK 68 85 MASS(ES) 96 AXILLARY REGION-RIGHT WEEK 68 89 MASS(ES) 95 INGUINAL REGION-RIGHT WEEK 89 58 MASS(ES) 61 VENTRAL-ABDOMINAL-RIGHT WEEK 58 62 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 58 63 MASS(ES) 64 VENTRAL-ABDOMINAL-RIGHT WEEK 58 65 MASS(ES) 69 VENTRAL-ABDOMINAL-RIGHT WEEK 58 70 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 58 50 X 40MM 40 X 40MM 15 X 15MM 15 X 10MM 15 X 20MM 20 X 20MM 30 X 30MM 40 X 40MM 1191 Covance 6329-183 3M T-6295 PAGE: 646 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C93131 C93132 F5 491 71 TO 526 76 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 58 533 77 TO 540 78 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 58 547 79 TO 554 80 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 58 561 81 TO 582 84 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 58 589 85 TO 666 96 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 58 F5 673 97 TO 680 98 MASS(ES) VENTRAL-ABDOMINAL WEEK 97 687 99 MASS(ES) VENTRAL-ABDOMINAL WEEK 97 694 100 MASS(ES) TO 715 103 VENTRAL-ABDOMINAL WEEK 97 30 X 30MM 35 X 40MM 40 X 50MM 50 X 40MM 50 X 50MM 20 X 20MM 30 X 30MM 30 X 35MM 1192 Covance 6329-183 3M T-6295 PAGE: 647 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 10 MASS(ES) 10 VENTRAL-ABDOMINAL WEEK 97 58 MASS(ES) 61 VENTRAL-ABDOMINAL-RIGHT WEEK 58 48 MASS(ES) 49 SHOULDER-LEFT WEEK 48 50 MASS(ES) 53 SHOULDER-LEFT WEEK 48 54 MASS(ES) 56 SHOULDER-LEFT WEEK 48 57 MASS(ES) SHOULDER-LEFT WEEK 48 58 MASS(ES) 60 SHOULDER-LEFT WEEK 48 61 MASS(ES) SHOULDER-LEFT WEEK 48 30 X 40MM 10 X 10MM 15 X 20MM 30 X 30MM 40 X 40MM 50 X 55MM 60 X 65MM 60 X 90MM 1193 Covance 6329-183 3M T-6295 PAGE: 648 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 62 MASS(ES) SHOULDER-LEFT WEEK 48 63 MASS(ES) SHOULDER-LEFT WEEK 48 64 MASS(ES) SHOULDER-LEFT WEEK 48 65 MASS(ES) 68 SHOULDER-LEFT WEEK 48 76 MASS(ES) 77 VENTRAL-ABDOMINAL-LEFT WEEK 74 85 MASS(ES) 90 AXILLARY REGION-LEFT WEEK 85 77 MASS(ES) 83 VENTRAL-ABDOMINAL-LEFT WEEK 77 68 MASS(ES) VENTRAL-ABDOMINAL WEEK 68 75 X 90MM 80 X 85MM 80 X 100MM 100 X 100MM 10 X 10MM 20 X 20MM 30 X 30MM 10 X 10MM 1194 Covance 6329-183 3M T-6295 PAGE: 649 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 102 MASS(ES) 105 AXILLARY REGION-RIGHT WEEK 102 104 MASS(ES) 105 INGUINAL REGION-RIGHT WEEK 104 93 MASS(ES) 95 VENTRAL-ABDOMINAL-RIGHT WEEK 93 96 MASS(ES) 101 VENTRAL-ABDOMINAL-RIGHT WEEK 93 102 MASS(ES) 105 VENTRAL-ABDOMINAL-RIGHT WEEK 93 102 MASS(ES) 105 AXILLARY REGION-RIGHT WEEK 102 100 MASS(ES) 104 VENTRAL-ABDOMINAL-LEFT WEEK 100 105 MASS(ES) 105 VENTRAL-ABDOMINAL-LEFT WEEK 100 10 X 10MM 10 X 10MM 10 X 10MM 15 X 15MM 15 X 20MM 10 X 10MM 15 X 15MM 20 X 20 MM 1195 Covance 6329-183 3M T-6295 PAGE: 650 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS CATEGORY KEYWORD WK QUALIFIER FREE-TEXT COMMENT 91 MASS(ES) 93 AXILLARY REGION-LEFT WEEK 91 94 MASS(ES) 99 AXILLARY REGION-LEFT WEEK 91 100 MASS(ES) 105 AXILLARY REGION-LEFT WEEK 91 100 MASS(ES) 102 VENTRAL-CERVICAL-LEFT WEEK 100 103 MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 100 104 MASS(ES) 105 VENTRAL-CERVICAL-LEFT WEEK 100 91 MASS(ES) 92 AXILLARY REGION-LEFT WEEK 89 93 MASS(ES) 101 AXILLARY REGION-LEFT WEEK 89 10 X 10MM 15 X 15MM 20 X 20MM 10 X 10MM 15 X 15MM 15 X 15MM 10 X 10MM 15 X 15MM 1196 Covance 6329-183 3M T-6295 PAGE: 651 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 102 MASS(ES) 104 AXILLARY REGION-LEFT WEEK 89 105 MASS(ES) 105 AXILLARY REGION-LEFT WEEK 89 81 MASS(ES) 85 INGUINAL REGION-LEFT WEEK 79 86 MASS(ES) INGUINAL REGION-LEFT WEEK 79 87 MASS(ES) INGUINAL REGION-LEFT WEEK 79 88 MASS(ES) INGUINAL REGION-LEFT WEEK 79 89 MASS(ES) 91 INGUINAL REGION-LEFT WEEK 79 92 MASS(ES) 93 INGUINAL REGION-LEFT WEEK 79 20 X 20MM 30 X 20MM 10 X 10MM 15 X 15MM 30 X 30MM 23 X 45MM 35 X 40MM 45 X 50MM 1197 Covance 6329-183 3M T-6295 PAGE: 652 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE- TEXT COMMENT C93151 C93152 C93153 F5 652 94 MASS(ES) INGUINAL REGION-LEFT WEEK 79 659 95 TO 673 97 MASS(ES) INGUINAL REGION-LEFT WEEK 79 680 98 MASS(ES) TO 701 101 INGUINAL REGION-LEFT WEEK 79 708 102 MASS(ES) TO 733 105 INGUINAL REGION-LEFT WEEK 79 F5 365 53 MASS(ES) AXILLARY REGION-RIGHT WEEK 53 372 54 TO 442 64 MASS(ES) AXILLARY REGION-RIGHT WEEK 53 449 65 TO 456 66 MASS(ES) AXILLARY REGION-RIGHT WEEK 53 F5 631 91 TO 645 93 MASS(ES) DORSAL WEEK 91 45 X 70MM 70 X 55MM 70 X 80MM 70 X 100MM 10 X 10MM 20 X 20MM 25 X 25MM 10 X 10MM 1198 Covance 6329-183 3M T-6295 PAGE: 653 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C93153 C93156 F5 652 94 TO 666 96 MASS(ES) DORSAL WEEK 91 673 97 TO 680 98 MASS(ES) DORSAL WEEK 91 687 99 MASS(ES) TO 715 103 DORSAL WEEK 91 722 104 MASS(ES) TO 732 105 DORSAL WEEK 91 F5 582 84 TO 596 86 MASS(ES) VENTRAL -ABDOMINAL WEEK 84 365 53 TO 372 54 MASS(ES) VENTRAL -CERVICAL WEEK 53 379 55 TO 421 61 MASS(ES) VENTRAL -CERVICAL WEEK 53 428 62 MASS(ES) VENTRAL-CERVICAL WEEK 53 15 X 20MM 25 X 20MM 30 X 20MM 40 X 40MM 10 X 10MM 10 X 10MM 15 X 20MM 15 X 35MM 1199 Covance 6329-183 3M T-6295 PAGE: 654 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 63 MASS(ES) VENTRAL-CERVICAL WEEK 53 64 MASS(ES) VENTRAL-CERVICAL WEEK 53 65 MASS(ES) 69 VENTRAL-CERVICAL WEEK 53 70 MASS(ES) VENTRAL-CERVICAL WEEK 53 71 MASS(ES) 80 VENTRAL-CERVICAL WEEK 53 81 MASS(ES) VENTRAL-CERVICAL WEEK 53 82 MASS(ES) 85 VENTRAL-CERVICAL WEEK 53 86 MASS(ES) 86 VENTRAL-CERVICAL WEEK 53 20 X 30MM 30 X 30MM 30 X 40MM 45 X 55MM 40 X 50MM 50 X 50MM 50 X 40MM 40 X 50MM 1200 Covance 6329-183 3M T-6295 PAGE: 655 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C93158 F5 491 71 TO 498 72 MASS(ES) INGUINAL REGION-RIGHT WEEK 71 505 73 TO 512 74 MASS(ES) INGUINAL REGION-RIGHT WEEK 71 519 75 TO 526 76 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 71 533 77 TO 554 80 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 71 561 81 TO 603 87 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 71 610 88 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 71 617 89 TO 680 98 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 71 687 99 MASS(ES) TO 701 101 VENTRAL-ABDOMINAL-RIGHT WEEK 71 10 X 10MM 15 X 15MM 15 X 15MM 20 X 20MM 30 X 20MM 60 X 50MM 50 X 40MM 70 X 40MM 1201 Covance 6329-183 3M T-6295 PAGE: 656 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 102 MASS(ES) 105 VENTRAL-ABDOMINAL-RIGHT WEEK 71 102 MASS(ES) 105 INGUINAL REGION-LEFT WEEK 102 77 MASS(ES) 87 INGUINAL REGION-LEFT WEEK 77 88 MASS(ES) 91 INGUINAL REGION-LEFT WEEK 77 92 MASS(ES) 94 INGUINAL REGION-LEFT WEEK 77 95 MASS(ES) INGUINAL REGION-LEFT WEEK 77 96 MASS(ES) INGUINAL REGION-LEFT WEEK 77 97 MASS(ES) INGUINAL REGION-LEFT WEEK 77 70 X 60MM 10 X 10MM 10 X 10MM 15 X 15MM 25 X 20MM 30 X 20MM 40 X 30MM 30 X 20MM 1202 Covance 6329-183 3M T-6295 PAGE: 657 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE- TEXT COMMENT C93164 C93165 C93173 F5 680 98 MASS(ES) TO 694 100 INGUINAL REGION-LEFT WEEK 77 701 101 MASS(ES) TO 715 103 INGUINAL REGION-LEFT WEEK 77 722 104 MASS(ES) TO 731 105 INGUINAL REGION-LEFT WEEK 77 F5 540 78 TO 652 94 MASS(ES) AXILLARY REGION-RIGHT WEEK 78 645 93 TO 652 94 MASS(ES) VENTRAL-THORACIC WEEK 93 659 95 MASS(ES) TO 701 101 VENTRAL-THORACIC WEEK 93 708 102 MASS(ES) TO 733 105 VENTRAL-THORACIC WEEK 93 F5 610 TO 624 90 CO CO MASS(ES) SHOULDER-RIGHT WEEK 88 40 X 25MM 50 X 30MM 65 X 40MM 10 X 10MM 20 X 20MM 30 X 20MM 30 X 30MM 15 X 25M 1203 Covance 6329-183 3M T-6295 PAGE: 658 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS CATEGORY KEYWORD WK QUALIFIER FREE-TEXT COMMENT 91 MASS(ES) SHOULDER-RIGHT WEEK 88 92 MASS(ES) 99 SHOULDER-RIGHT WEEK 88 100 MASS(ES) 102 SHOULDER-RIGHT WEEK 88 100 MASS(ES) 101 VENTRAL-THORACIC WEEK 100 102 MASS(ES) 104 VENTRAL-THORACIC WEEK 100 105 MASS(ES) 105 VENTRAL-THORACIC WEEK 100 75 MASS(ES) 78 VENTRAL-ABDOMINAL-LEFT WEEK 75 79 MASS(ES) 80 VENTRAL-ABDOMINAL-LEFT WEEK 75 15 X 20MM 20 X 25MM 20 X 30MM 15 X 15MM 20 X 20MM 30 X 20 MM 20 X 20MM 30 X 30MM 1204 Covance 6329-183 3M T-6295 PAGE: 659 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 81 MASS(ES) 84 VENTRAL-ABDOMINAL-LEFT WEEK 75 85 MASS(ES) 87 VENTRAL-ABDOMINAL-LEFT WEEK 75 88 MASS(ES) 91 VENTRAL-ABDOMINAL-LEFT WEEK 75 92 MASS(ES) 92 VENTRAL-ABDOMINAL-LEFT WEEK 75 72 MASS(ES) 73 INGUINAL REGION-LEFT WEEK 72 74 MASS(ES) INGUINAL REGION-LEFT WEEK 72 75 MASS(ES) INGUINAL REGION-LEFT WEEK 72 76 MASS(ES) INGUINAL REGION-LEFT WEEK 72 40 X 40MM 50 X 40MM 60 X 60MM 60 X 50MM 10 X 10MM 15 X 15MM 15 X 15MM 15 X 15MM 1205 Covance 6329-183 3M T-6295 PAGE: 660 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS CATEGORY KEYWORD WK QUALIFIER FREE-TEXT COMMENT 77 MASS(ES) INGUINAL REGION-LEFT WEEK 72 78 MASS(ES) INGUINAL REGION-LEFT WEEK 72 79 MASS(ES) INGUINAL REGION-LEFT WEEK 72 80 MASS(ES) INGUINAL REGION-LEFT WEEK 72 81 MASS(ES) INGUINAL REGION-LEFT WEEK 72 82 MASS(ES) INGUINAL REGION-LEFT WEEK 72 83 MASS(ES) INGUINAL REGION-LEFT WEEK 72 84 MASS(ES) INGUINAL REGION-LEFT WEEK 72 15 X 15MM 20 X 20MM 20 X 20MM 20 X 20MM 20 X 20MM 30 X 20MM 30 X 20MM 30 X 20MM 1206 Covance 6329-183 3M T-6295 PAGE: 661 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS CATEGORY KEYWORD WK QUALIFIER FREE-TEXT COMMENT 85 MASS(ES) INGUINAL REGION-LEFT WEEK 72 86 MASS(ES) INGUINAL REGION-LEFT WEEK 72 87 MASS(ES) INGUINAL REGION-LEFT WEEK 72 88 MASS(ES) INGUINAL REGION-LEFT WEEK 72 88 MASS(ES) INGUINAL REGION-LEFT WEEK 72 88 MASS(ES) INGUINAL REGION-LEFT WEEK 72 88 MASS(ES) INGUINAL REGION-LEFT WEEK 72 79 MASS(ES) 88 INGUINAL REGION-RIGHT WEEK 79 30 X 20MM 30 X 20MM 30 X 20MM 30 X 20MM 30 X 20MM 30 X 20MM 30 X 20MM 10 X 10MM 1207 Covance 6329-183 3M T-6295 PAGE: 662 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C93179 C93180 F5 659 95 TO 680 98 MASS(ES) AXILLARY REGION-LEFT WEEK 95 687 99 MASS(ES) AXILLARY REGION-LEFT WEEK 95 694 100 MASS(ES) TO 701 101 AXILLARY REGION-LEFT WEEK 95 708 102 MASS(ES) TO 715 103 AXILLARY REGION-LEFT WEEK 95 722 104 MASS(ES) TO 733 105 AXILLARY REGION-LEFT WEEK 95 F5 386 56 TO 407 59 MASS(ES) VENTRAL-THORACIC-LEFT WEEK 56 414 60 MASS(ES) VENTRAL-THORACIC-LEFT WEEK 56 421 61 MASS(ES) VENTRAL-THORACIC-LEFT WEEK 56 15 X 15MM 20 X 15MM 20 X2 0MM 40 X 50MM 40 X 40MM 30 X 50MM 40 X 60MM 80 X 60MM 1208 Covance 6329-183 3M T-6295 PAGE: 663 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C93180 F5 428 62 428 62 435 63 TO 435 63 MASS(ES) VENTRAL-THORACIC-LEFT WEEK 56 MASS(ES) VENTRAL-THORACIC-LEFT WEEK 56 MASS(ES) VENTRAL-THORACIC-LEFT WEEK 56 90 X 80MM 80 X 60MM 110 X 80MM Covance 6329-183 3M T-6295 PAGE: 664 1209 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 665 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F6 DOSE: 20 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C93181 U 106 APPEARANCE MALOCCLUSION NORMAL NO REMARKABLE OBSERVATIONS C93182 U 106 SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE ROUGH HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) AXILLARY REGION-LEFT WEEK 102 190, 281, 407, 498, 589, 680, 197, 288, 414, 505, 596, 687, 204, 2 1 1 , 218, 295, 302, 309, 421,. 428, 435, 512, 519, 526, 603,. 610, 617, 694, 701, 708, 225, 316, 442, 533, 624, 715, 232, 358, 449, 540, 631, 722, 239 , 246, 365 , 372, 456 , 463, 547 , 554, 638 , 645, 729 , 736 253, 379, 470, 561, 652, 260, 386, 477, 568, 659, 267, 274 393, 400 484, 491 575, 582 666, 673 1 , 8 ,r 15, 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 323, 330 337, 344, 351 393 393 1 , 8 ,r 15, 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190, 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281, 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372, 379 386, 400, 407,. 414, 421, 428, 435, 442 , 449, 456, 463, 470, 477 484, 491, 498, 505, 512, 519, 526, 533 , 540, 547, 554, 561, 568 575, 582, 589,. 596, 603, 610, 617, 624 , 631, 638, 645, 652, 659 666, 673, 680, 687, 694, 701 708C, 715C, 722C, 729C, 736C 1210 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 666 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F6 DOSE: 20 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C93183 U 106 NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) INGUINAL REGION-LEFT WEEK 91 U 106 SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE SORE/SCAB PAW-HIND-LEFT NORMAL NO REMARKABLE OBSERVATIONS WART LIKE LESION(S) MOUTH WEEK 102 1 , 8 , 15, 2 2 , 29, 36, 43 , 50, 57, 64, 71 , 78, 85 , 92, 99, 106 113, 1 2 0 , 127, 134 , 141, 148, 155, 162, 169, 176 , 183, 190 , 197 204, 2 1 1 , 218, 225 , 232, 239, 246, 253, 260, 267 , 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344, 351, 358 , 365, 372 , 379 386, 393, 400, 407 , 414, 421, 428, 435, 442, 449 , 456, 463 , 470 477, 484, 491, 498 , 505, 512, 519, 526, 533, 540 , 547, 554 , 561 568, 575, 582, 589 , 596, 603, 610, 617, 624 631C, 638C , 645C, 652C, 659C, 666C , 673C , 680C, 687C, 694C , 701C 708C, 715C, 722C, 729C, 736C 190, 197, 204, 2 1 1 , 218, 225, 232, 239, 246, 253, 260, 267, 274 561, 568, 708 1, 8 ,r 15, 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127,. 134, 141, 148, 155, 162, 169, 176, 183, 281, 288 295, 302, 309,. 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470 477, 484, 491, 498, 505, 512, 519, 526, 533, 540, 547, 554, 575 582, 589, 596, 603, 610, 617, 624, 631, 638, 645, 652, 659, 666 673, 680, 687,. 694, 701 708, 715, 722,. 729, 736 1211 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 667 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F6 DOSE: 20 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C93185 U 106 APPEARANCE MALOCCLUSION C93186 M EYES OPAQUE EYE-LEFT RED DISCHARGE EYE-LEFT NORMAL NO REMARKABLE OBSERVATIONS 75 NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) VENTRAL-CERVICAL WEEK 45 197, 358, 449, 540, 631, 722, 204, 365, 456, 547, 638, 729, 2 1 1 , 218, 372, 379, 463, 470, 554, 561, 645, 652, 736 225, 386, 477, 568, 659, 232, 239, 393, 400, 484, 491, 575, 582, 666, 673, 246, 407, 498, 589, 680, 253, 414, 505, 596, 687, 260, 421, 512, 603, 694, 267, 428, 519, 610, 701, 274, 435, 526, 617, 708, 351 442 533 624 715 722, 729, 736 288, 295, 302, 309, 316, 323, 330, 337, 344 1 , 8 , 15, 2 2 , 29, 36, 43,, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127,, 134, 141, 148, 155,. 162, 169, 176, 183, 190 , 281 1 , 8 , 15, 2 2 , 29, 36, 43,, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127,, 134, 141, 148, 155,. 162, 169, 176, 183, 190 , 197 204, 2 1 1 , 218,, 225, 232, 239, 246,. 253, 260, 267, 274, 281 , 288 295, 302 309C, 316C, 323C, 330C, 337C, 344C, 351C, 358C, 365C, 372C, 379C 386C, 393C, 400C, 407C, 414C, 421C, 428C, 435C, 442C, 449C, 456C 463C, 470C, 477C, 484C, 491C, 498C, 505C, 512C, 519-520C 1212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 668 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F6 DOSE: 20 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C93187 M 104 APPEARANCE HEAD TILT LEFT MALOCCLUSION BEHAVIOR ATAXIC <UNSPECIFIED> HYPOACTIVE <UNSPECIFIED> EXCRETION FEW FECES SKIN & PELAGE PALE ENTIRE BODY ROUGH HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) AXILLARY REGION-RIGHT WEEK 102 INGUINAL REGION-LEFT WEEK 81 725 715, 722, 725 722, 725 725 617, 705, 708, 715, 722, 725 725 722, 725 1 , 8 ,r 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407 , 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498 , 505, 512, 519, 526 , 533, 540, 547, 554 708C, 715C, 722C, 725C 561C, 568C, 575C, 582C, 589C, 596C, 603C, 610C, 617C, 624C, 631C 1213 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 669 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F6 (CONTINUED FROM PREVIOUS PAGE) C93187 M 104 MASS(ES) INGUINAL REGION-LEFT WEEK 81 INGUINAL REGION-RIGHT WEEK 101 C93188 D 105 SKIN & PELAGE SORE/SCAB AXILLARY REGION-LEFT NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) AXILLARY REGION-LEFT WEEK 70 DOSE: 20 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 638C, 64 5C, 652C, 659C, 666C, 673C, 680C, 687C, 694C, 701C, 705C 708C, 715C, 722C, 725C 701C, 705C 729, 734 1 , 8 ,r 15, 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477 484C, 491C, 498C, 505C, 512C, 519C, 526C, 533C, 54 0C, 54 7C, 554C 561C, 568C, 575C, 582C, 58 9C, 596C, 603C, 610C, 617C, 624C, 631C 638C, 645C, 652C, 659C, 666C, 673C, 680C, 687C, 694C, 701C, 708C 715C, 722C, 729C, 734C 1214 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 670 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F6 DOSE: 20 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C93189 M 87 APPEARANCE HEAD TILT LEFT HUNCHED MALOCCLUSION MISSING TEETH THIN BEHAVIOR HYPOACTIVE <UNSPECIFIED> EXCRETION FEW FECES EYES RED DISCHARGE EYE-RIGHT NORMAL NO REMARKABLE OBSERVATIONS 605 605 232, 323, 414, 505, 596, 239 330 421 512 603 246, 337, 428, 519, 605 253, 344, 435, 526, 260, 351, 442, 533, 267, 358, 449, 540, 274, 365, 456, 547, 281, 372, 463, 554, 288, 379, 470, 561, 295, 386, 477, 568, 302, 393, 484, 575, 309, 400, 491, 582, 316 407 498 589 239, 246 253, 260 589, 596 603, 605 605 605 155 1 , 8 , 15 2 2 , 29, 36, 43,, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120 127, 134 , 141, 148, 162, 169 , 176, 183, 190, 197 , 204 2 1 1 , 218 225 1215 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 671 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F6 DOSE: 20 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C93190 U 106 APPEARANCE THIN EXCRETION FEW FECES EYES RED DISCHARGE EYES SKIN & PELAGE ROUGH HAIR COAT SORE/SCAB PAW-HIND-LEFT NORMAL NO REMARKABLE OBSERVATIONS 701, 708, 715, 722 680 701, 708 722, 729, 736 561 1 , 8 ,r 15, 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 568 575, 582, 589,. 596, 603, 610, 617, 624 , 631, 638, 645, 652 , 659 666, 673, 687,. 694 1216 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 672 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F6 DOSE: 20 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C93191 M 91 BEHAVIOR HYPOACTIVE <UNSPECIFIED> RECUMBENT LATERAL EYES RED DISCHARGE EYES RESPIRATION AUDIBLE LABORED NORMAL NO REMARKABLE OBSERVATIONS C93192 U 106 APPEARANCE MALOCCLUSION SKIN & PELAGE SORE/SCAB MOUTH NORMAL NO REMARKABLE OBSERVATIONS 633 633 633 633 633 1 , 8 ,r 15, 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582, 589, 596, 603, 610, 617 , 624, 631 155 64, 71, 78 1, 8 , 15, 22, 29, 36, 43, 50, 57, 85, 92 99, 106, 113, 120, 127 1217 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 673 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F6 (CONTINUED FROM PREVIOUS PAGE) C93192 U 106 NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 91 M 76 BEHAVIOR HYPOACTIVE <UNSPECIFIED> EXCRETION FEW FECES NONFORMED FECES SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE YELLOW HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS DOSE: 20 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 134, 232, 323, 414, 505, 596, 141, 239, 330, 421, 512, 603, 14 8 ,. 162, 246,. 253, 337,. 344, 428,. 435, 519,. 526, 610, 617, 169, 260, 351, 442, 533, 624 176, 267, 358, 449, 540, 183, 274, 365, 456, 547, 190, 281, 372, 463, 554, 197, 288, 379, 470, 561, 204 , 2 1 1 , 218 , 225 295 , 302, 309 , 316 386 , 393, 400 , 407 477 , 484, 491 , 498 568 , 575, 582 , 589 631C, 638C , 645C, 652C, 659C, 666C , 673C , 68 0C, 687C, 694C , 701C 708C, 715C!, 722C, 72 9C, 736C 530 530 530 512, 519, 526, 529-530 512, 519, 526, 529-530 1 , 8 , 15, 2 2 , 29, 36, 43 , 50, 57, 64, 71 , 78 , 85 , 92, 99, 106 113, 1 2 0 , 127,. 134, 141, 148, 155, 162, 169, 176 , 183, 190 , 197 204, 2 1 1 , 218,. 225, 232, 239, 246, 253, 260, 267 , 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344, 351, 358 , 365, 372 , 379 386, 393, 400,. 407, 414, 421, 428, 435, 442, 449 , 456, 463 , 470 1218 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 674 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F6 (CONTINUED FROM PREVIOUS PAGE) C93193 M 76 NORMAL NO REMARKABLE OBSERVATIONS C93194 M 92 SKIN & PELAGE ALOPECIA RED HAIR COAT VENTRAL-ABDOMINAL ROUGH HAIR COAT SORE/SCAB DORSAL-CERVICAL LIMBS-HIND VENTRAL-ABDOMINAL-RIGHT VENTRAL-THORACIC NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) AXILLARY REGION-LEFT WEEK 76 INGUINAL REGION-RIGHT WEEK 81 VENTRAL-ABDOMINAL-LEFT WEEK 82 VENTRAL-ABDOMINAL-RIGHT WEEK 81 DOSE: 20 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 477, 484, 491, 498, 505 575, 582, 589, 596 , 603, 610, 617, 624 , 631, 638 498, 505 512, 519, 526, 533 , 540, 547, 554, 561 , 568, 575, 582, 589 , 596 603, 610, 617, 624 , 631, 638 561, 568, 575, 582 , 589, 596, 603 582 582, 589, 596, 603 , 610, 617, 624, 631 , 638 561, 568, 575, 582 , 589, 596, 603, 610 , 617, 624, 631, 638 1 , 8 ,r 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407 , 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491 526, 533, 540 568C, 575C, 582C, 589C, 596C 568, 575, 582, 589, 596, 603C, 610C, 617C, 624C, 631C, 638C 561C 1219 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 675 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F6 (CONTINUED FROM PREVIOUS PAGE) C93194 M 92 WART LIKE LESION(S) HEAD-MAXILLARY-RIGHT WEEK 76 C93195 D 71 NORMAL NO REMARKABLE OBSERVATIONS C93196 M 88 BEHAVIOR HYPOACTIVE <UNSPECIFIED> EXCRETION FEW FECES EYES RED DISCHARGE EYES NORMAL NO REMARKABLE OBSERVATIONS DOSE: 20 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 526 1, 8 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407 , 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491 613-614 613-614 613-614 1 , 8 ,r 15, 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582, 589, 596, 603, 610 1220 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 676 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F6 DOSE: 20 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C93197 U 106 SKIN & PELAGE SORE/SCAB PAW-HIND-LEFT NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) AXILLARY REGION-LEFT WEEK 99 INGUINAL REGION-LEFT WEEK 93 WEEK 95 C93198 U 106 NORMAL NO REMARKABLE OBSERVATIONS 631, 638, 645,. 652, 659, 666, 673, 680 , 687, 694, 701, 708 , 715 722, 729, 736 1 , 8 ,r 15, 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582, 589, 596, 603, 610, 617 , 624 687, 694C, 701C, 708C, 715C, 722C, 72 9C, 736C 645C, 722C, 659C, 736C 652C, 72 9C, 666C, 659C, 736C 673C, 666C, 680C, 673C, 687C, 680C, 694C, 687C, 701C, 694C, 708C, 701C, 715C, 708C, 722C, 715C 72 9C 1 , 8 ,r 15, 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0 , 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582, 589, 596, 603, 610, 617 , 624, 631, 638, 645 1221 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 677 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F6 (CONTINUED FROM PREVIOUS PAGE) C93198 U 106 MASS(ES) AXILLARY REGION-RIGHT WEEK 94 M 65 APPEARANCE HEAD TILT RIGHT THIN NORMAL NO REMARKABLE OBSERVATIONS C93200 M 96 APPEARANCE THIN BEHAVIOR HYPOACTIVE <UNSPECIFIED> DISCHARGE NASAL RED IN COLOR EXCRETION FEW FECES NO FECES DOSE: 20 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 666652, 659, , 673 , 680, 687, 694C , 701C, 708C, 715C, 722C , 72 9C 736C 449- 450 442, 449- 450 1 22, 8 , 15, , 29, 36, 43,r 50, 57, 64, 71, 78, 85 , 92, 99, 106 120113, , 127, 134 , 141, 148, 155, 162, 169, 176 , 183, 190 , 197 211204, , 218, 225 , 232, 239, 246, 253, 260, 267 , 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344, 351, 358 , 365, 372 , 379 386, 393, 400, 407 , 414, 421, 428, 435 671 666, 671 666 659 671 1222 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 678 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F6 (CONTINUED FROM PREVIOUS PAGE) C93200 M 96 EYES RED DISCHARGE EYE-RIGHT EYES SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE YELLOW HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS C93201 U 106 APPEARANCE CONVULSIONS TREMORS ENTIRE BODY NORMAL NO REMARKABLE OBSERVATIONS DOSE: 20 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 561, 568, 575, 666 671 666 666, 671 1 , 8 ,r 15, 2 2 , 113, 1 2 0, 127 204, 2 1 1 , 218 295, 302, 309 386, 393, 400 477, 484, 491 589, 596, 603 9, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 134, 141, 148, 155, 162 , 169, 176, 183, 190, 197 225, 232, 239, 246, 253 , 260, 267, 274, 281, 288 316, 323, 330, 337, 344 , 351, 358, 365, 372, 379 407, 414, 421, 428, 435 , 442, 449, 456, 463, 470 498, 505, 512, 519, 526 , 533, 540, 547, 554, 582 610, 617, 624, 631, 638 , 645, 652 232, 239, 330, 400 687 1 , 8 ,r 15, 2 2 , 113, 1 2 0, 127 204, 2 1 1 , 218 309, 316, 323 414, 421, 428 505, 512, 519 596, 603, 610 9, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 134, 141, 148, 155, 162 , 169, 176, 183, 190, 197 225, 246, 253, 260, 267 , 274, 281, 288, 295, 302 337, 344, 351, 358, 365 , 372, 379, 386, 393, 407 435, 442, 449, 456, 463 , 470, 477, 484, 491, 498 526, 533, 540, 547, 554 , 561, 568, 575, 582, 589 617, 624, 631, 638, 645 , 680, 694, 701, 708, 715 1223 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 679 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F6 (CONTINUED FROM PREVIOUS PAGE) C93201 U 106 NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) AXILLARY REGION-RIGHT WEEK 94 C93202 U 106 NORMAL NO REMARKABLE OBSERVATIONS C93203 M 90 SKIN & PELAGE BLACK SKIN VENTRAL-ABDOMINAL-LEFT VENTRAL-ABDOMINAL-RIGHT SORE/SCAB VENTRAL-ABDOMINAL-LEFT VENTRAL-ABDOMINAL-RIGHT NORMAL NO REMARKABLE OBSERVATIONS DOSE: 20 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 722, 729, 736 652, 659, 666, 673 1, 8 15, 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582, 589, 596, 603, 610, 617 , 624, 631, 638, 645 , 652 659, 666, 673, 680, 687, 694, 701, 708 , 715, 722, 729, 736 526, 533 540 540, 547, 554, 561, 568, 575, 582, 589 , 596, 603, 610, 617 , 624 547, 554, 561,. 568, 575, 582, 589, 596 , 603, 610, 617, 624 1 , 8 ,r 15, 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421 1224 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 680 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F6 (CONTINUED FROM PREVIOUS PAGE) C93203 M 90 MASS(ES) AXILLARY REGION-LEFT WEEK 76 WEEK 81 AXILLARY REGION-RIGHT WEEK 82 INGUINAL REGION-LEFT WEEK 81 VENTRAL-ABDOMINAL-LEFT WEEK 69 VENTRAL-ABDOMINAL-RIGHT WEEK 62 VENTRAL-THORACIC-LEFT WEEK 81 D 92 NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 66 DOSE: 20 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 526, 533C, 540C, 547C, 554C, 561C, 568C, 575C, 582C, 58 9C, 596C 603C, 610C , 617C, 624C 561, 568, 575, 582, 589, 596, 603 568, 575 561, 568, 575, 582, 589C , 596C, 603C, 610C, 617C, 624C 477C, 484C , 491C, 498C, 505C, 512C , 519C, 526C, 533C 42 8C, 435C , 442C, 449C, 456C, 463C , 470C, 477C, 484C, 491C , 498C 505C, 512C , 519C, 526C, 533C, 540C , 547C, 554C, 561C, 568C , 575C 582C, 589C , 596C 561C, 568C , 575C, 582C 1 , 8 ,. 15, 22, 29, 36, 43 , 50, 113, 1 2 0, 127, 134, 141, 148, 204, 2 1 1 , 218, 225, 232, 239, 295, 302, 309, 316, 323, 330, 386, 393, 400, 407, 414, 421, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449 92, 99, 106 183 , 190 , 197 274 , 281 , 288 365 , 372 , 379 456C, 463C , 470C, 477C, 484C, 491C , 498C, 505C, 512C, 519C , 526C 533C, 540C , 547C, 554C, 561C, 568C , 575C, 582C, 589C, 596C , 603C 610C, 617C , 624C, 631C, 638C, 642C 1225 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 681 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F6 DOSE: 20 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C93205 U 106 NORMAL NO REMARKABLE OBSERVATIONS APPEARANCE THIN BEHAVIOR HYPOACTIVE <UNSPECIFIED> EXCRETION FEW FECES EYES RED DISCHARGE EYES NORMAL NO REMARKABLE OBSERVATIONS 1 , 8 ,r 15, 2 2 , 29, 36 , 43,r 50, 113, 1 2 0, 127,. 134, 141, 148, 204, 2 1 1 , 218, 225, 232, 239, 295, 302, 309,. 316, 323, 330, 386, 393, 400, 407, 414, 421, 477, 484, 491, 498, 505, 512, 568, 575, 582, 589, 596, 603, 659, 666, 673, 680, 687, 694, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 701, 708, 715, 722, 92, 99, 10 6 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 638, 645, 652 729, 736 701, 708, 715,. 722, 727 727 673, 701, 708,. 715, 722, 727 708, 722, 727 1 , 8 ,r 15, 2 2 , 29, 36 , 43,r 50, 113, 1 2 0, 127,. 134, 141, 148, 204, 2 1 1 , 218, 225, 232, 239, 295, 302, 309,. 316, 323, 330, 386, 393, 400, 407, 414, 421, 477, 484, 491, 498, 505, 512, 568, 575, 582, 589, 596, 603, 57, 64, 71, 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 519, 526, 533, 540, 610, 617, 624, 631, 92, 99, 10 6 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 638, 645, 652 1226 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 682 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F6 (CONTINUED FROM PREVIOUS PAGE) C93206 M 104 NORMAL NO REMARKABLE OBSERVATIONS C93207 M 41 APPEARANCE HUNCHED THIN EXCRETION NO FECES EYES RED DISCHARGE EYES NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) VENTRAL-THORACIC-RIGHT WEEK 10 C93208 D 83 BEHAVIOR RECUMBENT STERNAL DISCHARGE GENITAL RED IN COLOR DOSE: 20 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 659, 666, 278, 281 278, 281 278 680, 687,694 278, 281 1, 8 , 15, 22, 29, 36, 43, 50, 57 64, 71C, 78C , 85C, 92C, 99C, 106C, 113C, 12 0C, 12 7C, 134C, 141C 148C , 155C, 162C, 16 9C, 176C, 183C, 190C, 197C, 204C, 211C, 218C 225C , 232C, 239C, 246C, 253C, 260C, 267C, 274C, 278C, 281C 576 316 1227 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 683 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F6 (CONTINUED FROM PREVIOUS PAGE) C93208 D 83 RESPIRATION AUDIBLE IRREGULAR NORMAL NO REMARKABLE OBSERVATIONS C93209 U 106 NORMAL NO REMARKABLE OBSERVATIONS C93210 M 105 APPEARANCE MALOCCLUSION THIN BEHAVIOR HYPOACTIVE <UNSPECIFIED> DOSE: 20 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 576 576 1 , 8 ,r 15, 2 2 , 29, 36, 43,r 50, 113, 1 2 0, 127, 134 , 141, 148, 204, 2 1 1 , 218, 225 , 232, 239, 295, 302, 309, 323 , 330, 337, 393, 400, 407, 414 , 421, 428, 484, 491, 498, 505 , 512, 519, 575 57, 64, 71, 78, 85, 155, 162 , 169, 176, 246, 253 , 260, 267, 344, 351 , 358, 365, 435, 442 , 449, 456, 526, 533 , 540, 547, 92, 99, 106 183, 190 , 197 274, 281 , 288 372, 379 , 386 463, 470 , 477 554, 561 , 568 1 , 8 ,r 15, 2 2 , 29, 36, 43,r 50, 113, 1 2 0, 127, 134 , 141, 148, 204, 2 1 1 , 218, 225 , 232, 239, 295, 302, 309, 316 , 323, 330, 386, 393, 400, 407 , 414, 421, 477, 484, 491, 498 , 505, 512, 568, 575, 582, 589 , 596, 603, 659, 666, 673, 680 , 687, 694, 57, 64, 71, 78, 85, 155, 162 , 169, 176, 246, 253 , 260, 267, 337, 344 , 351, 358, 428, 435 , 442, 449, 519, 526 , 533, 540, 610, 617 , 624, 631, 701, 708 , 715, 722, 92, 99, 106 183, 190 , 197 274, 281 , 288 365, 372 , 379 456, 463 , 470 547, 554 , 561 638, 645 , 652 729, 736 141, 148, 414, 421 , 428, 435, 463, 470 , 477, 582, 589, 596 , 603 722, 729, 734 729, 734 1228 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 684 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F6 (CONTINUED FROM PREVIOUS PAGE) C93210 M 105 EXCRETION FEW FECES SKIN & PELAGE YELLOW HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) INGUINAL REGION-LEFT WEEK 94 INGUINAL REGION-RIGHT WEEK 88 C93211 U 106 APPEARANCE CONVULSIONS TREMORS ENTIRE BODY NORMAL NO REMARKABLE OBSERVATIONS DOSE: 20 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 705, 708, 722, 729, 734 722, 729, 734 1 , 8 ,r 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0, 127, 134 , 155, 162, 169, 176 , 183, 190, 197, 204 , 211 218, 225, 232, 239 , 246, 253, 260, 267 , 274, 281, 288, 295 , 302 309, 316, 323, 330 , 337, 344, 351, 358 , 365, 372, 379, 386 , 393 400, 407, 442, 449 , 456, 498, 505, 512 , 519, 526, 533, 540 , 547 554, 561, 568, 575 652C, 659C, 666C, 673C, 680C, 687C, 694C, 701C, 705C, 708C, 715C 722C, 729C, 734C 610C, 617C, 624C, 631C, 638C, 645C, 652C, 659C, 666C, 673C, 680C 687C, 694C, 701C, 705C, 708C, 715C, 722C, 729C, 734C 400, 407, 414, 421 , 428, 435, 442, 491 , 498, 512, 638, 645 , 652 722 680, 687 1 , 8 ,r 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 1229 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 685 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F6 (CONTINUED FROM PREVIOUS PAGE) C93211 U 106 NORMAL NO REMARKABLE OBSERVATIONS C93212 D MASS(ES) INGUINAL REGION-LEFT WEEK 104 84 SKIN & PELAGE SORE/SCAB LIMBS-HIND PAWS-HIND WARM TO TOUCH LIMBS-HIND NORMAL NO REMARKABLE OBSERVATIONS DOSE: 20 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 386, 393, 449, 456 , 463, 470, 477, 484 , 505, 519, 526, 533 547, 554, 561, 568 , 575, 582, 589, 596 , 603, 610, 617, 624 659, 666, 673, 694 , 701, 708, 715 722, 729, 736 533, 540, 547, 554 561, 568, 575, 582 , 584 526 1 , 8 ,r 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 113, 1 2 0, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 204, 2 1 1 , 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 386, 393, 400, 407 , 414, 421, 428, 435 , 442, 449, 456, 463 477, 484, 491, 498 , 505, 512, 519 1230 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 686 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F6 DOSE: 20 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C93213 M 84 APPEARANCE HEAD TILT LEFT HUNCHED MALOCCLUSION THIN TREMORS ENTIRE BODY BEHAVIOR ATAXIC <UNSPECIFIED> HYPOACTIVE <UNSPECIFIED> COLD TO TOUCH ENTIRE BODY DISCHARGE NASAL RED IN COLOR EXCRETION FEW FECES EYES RED DISCHARGE EYE-LEFT 586 586 113, 232, 323, 414, 505, 554, 1 2 0, 127, 239, 246, 330, 337, 421, 428, 512, 519, 561, 568, 134, 253, 344, 435, 526, 575, 141, 260, 351, 442, 533, 582, 148, 267, 358, 449, 540, 586 155, 274, 365, 456, 547, 190, 281, 372, 463, 554, 197 288 379 470 561 204, 211, 218, 225 295, 302, 309, 316 386, 393, 400, 407 477, 484, 491, 498 568, 575, 582, 586 586 561, 568, 575, 582, 586 561, 568, 575, 582, 586 586 561 554, 586 393 1231 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 687 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F6 (CONTINUED FROM PREVIOUS PAGE) C93213 M 84 SKIN & PELAGE PALE ENTIRE BODY NORMAL NO REMARKABLE OBSERVATIONS WART LIKE LESION(S) TAIL-PROXIMAL WEEK 76 D 98 NORMAL NO REMARKABLE OBSERVATIONS C93215 M 86 APPEARANCE THIN BEHAVIOR ATAXIC <UNSPECIFIED> HYPOACTIVE <UNSPECIFIED> DOSE: 20 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 586 1 , 8 ,r 15, 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 162, 169, 176, 183 526, 533, 540, 547, 554 1 , 8 ,r 15, 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582, 589, 596, 603, 610, 617 , 624, 631, 638, 645 , 652 659, 666, 673, 680, 684 582, 589, 596 596 575, 582, 589, 596 1232 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 688 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F6 (CONTINUED FROM PREVIOUS PAGE) C93215 M 86 EXCRETION FEW FECES SKIN & PELAGE SORE/SCAB PAW-HIND-RIGHT NORMAL NO REMARKABLE OBSERVATIONS C93216 U WART LIKE LESION(S) HEAD-MAXILLARY-LEFT WEEK 74 106 SKIN & PELAGE SORE/SCAB PAW-HIND-LEFT VENTRAL-ABDOMINAL-RIGHT NORMAL NO REMARKABLE OBSERVATIONS DOSE: 20 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 551, 554 568, 575 1 , 8 , 15 113, 120 204, 211 295, 302 386, 393 477, 484 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 400, 407 , 414, 421, 428, 435 , 442, 449, 456, 463 , 470 491, 498 , 505 512, 519 526, 533 , 540, 547, 551, 554 , 561, 568, 575, 582 , 589 666, 673, 680 631, 638, 645, 652 , 659, 694, 701, 708 , 715, 722, 729, 736 1 , 8 ,r 15, 2 2 , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 2 1 1 , 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407 , 414 1233 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 689 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F6 (CONTINUED FROM PREVIOUS PAGE) C93216 U 106 MASS(ES) AXILLARY REGION-RIGHT WEEK 81 WEEK 93 EAR-RIGHT WEEK 61 C93217 D VENTRAL -ABDOMINAL-LEFT WEEK 101 VENTRAL -ABDOMINAL-RIGHT WEEK 79 WEEK 81 WEEK 96 91 NORMAL NO REMARKABLE OBSERVATIONS DOSE: 20 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 561C, 568C, 575C, 582C, 589C , 596C, 603C, 610C, 617C, 624C , 631C 638C, 645C, 652C, 659C, 666C , 673C, 680C, 687C, 694C, 701C , 708C 715C,. 722C, 729C, 736C 645, 652, 659C, 666C, 673C, 680C, 687C, 694C, 701C, 708C, 715C 722C,. 729C, 736C 421, 428, 435, 442, 449, 456 , 463, 470, 477, 484, 491, 498 , 505 512, 519, 526, 53 3, 540, 547 , 554, 561, 568, 575, 582, 589 , 596 603, 610C, 617C, 624C, 631, 638, 64 5, 652, 659, 666, 673, 680 687, 694, 701, 70 8 , 715, 722 , 729, 736 701C, 708C, 715C, 722C, 729C , 736C 547C 561C, 568C, 575C, 582C, 589C , 596C, 603C, 610C, 617C, 624C , 631C 638C 666, 673C, 680C, 687C, 694C, 701C, 708C, 715C, 722C, 729C, 736C 1 , 8 ,r 15, 2 2 , 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 1 2 0, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190, 197 204, 2 1 1 , 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281, 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463, 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554, 561 568, 575, 582, 589, 596, 603, 610, 617 , 624, 631, 635 1234 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 690 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F6 DOSE: 20 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C93218 M 90 SKIN & PELAGE SORE/SCAB AXILLARY REGION-LEFT NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) AXILLARY REGION-LEFT WEEK 50 C93219 U INGUINAL REGION-LEFT WEEK 48 106 SKIN & PELAGE SORE/SCAB VENTRAL-THORACIC-RIGHT NORMAL NO REMARKABLE OBSERVATIONS 484, 491, 498, 505, 512, 519, 526,. 533, 540, 547, 554, 561, 568 575, 582, 589,. 596, 603, 610, 617, 624 1 22 6, 8,r 15, , 29, 3 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 120113, , 127,. 134, 141, 148, 155,. 162, 169, 176, 183, 190, 197 211204, , 218, 225, 232, 239, 246,. 253, 260, 267, 274, 281, 288 295, 302, 309,. 316, 323 344, 351, 358, 36 5C, 372C, 379C, 386C, 393C, 40 o n o 7C, 414C 421C, 428C, 435C, 442C, 44 9C, 456C, 463C, 470C, 477C, 484C, 491C 498C, 505C, 512C, 519C, 526C, 533C, 54 0C, 54 7C, 554C, 561C, 568C 575C, 582C, 58 9C, 596C, 603C, 610C, 617C, 624C 330,337 666659, , 673, 680 , 687, 694, 701, 708 , 715, 722, 729, 736 1 22, 8,r 15, , 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 120113, , 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190, 197 211204, , 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281, 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372, 379 386, 393, 400, 407 , 414, 421, 428, 435 , 442, 449, 456, 463, 470 477, 484, 491, 498 , 505, 512, 519, 533 , 540, 547, 554, 582 1235 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 691 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F6 (CONTINUED FROM PREVIOUS PAGE) C93219 U 106 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 101 VENTRAL-THORACIC-RIGHT WEEK 76 WEEK 81 WEEK 85 C93220 U 106 APPEARANCE MALOCCLUSION NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) VENTRAL-ABDOMINAL WEEK 97 DOSE: 20 PPM DAYS 1-736 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 701, 708 526 561, 568, 575 58 9C, 596C, 603C, 666C, 673C, 680C, 610C, 687C, 617C, 694C, 624C, 701C, 631C, 708C 638C, 645C, 652C 659C 358, 449, 540, 631, 722, 365, 456, 547, 638, 729, 372, 463, 554, 645, 736 379, 470, 561, 652, 386, 477, 568, 659, 393, 400, 484, 491, 575, 582, 666, 673, 407, 498, 589, 680, 414, 505, 596, 687, 421, 512, 603, 694, 428, 519, 610, 701, 435, 526, 617, 708, 442 533 624 715 1 , 8 , 15, 2 2 , 29, 36, 43 , 50, 57, 64, 71, 78,, 85, 92, 99, 106 113, 1 2 0, 127,. 134, 141, 148, 155, 162, 169, 176, 183,. 190, 197 204, 2 1 1 , 218,. 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309,. 316, 323, 330, 337, 344, 351 673C, 680C', 687C, 694C, 701C, 708C , 715C, 722C, 729C, 736C 1236 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 10. MASS(ES) 10 AXILLARY REGION-LEFT WEEK 102 91 MASS(ES) INGUINAL REGION-LEFT WEEK 91 92 MASS(ES) INGUINAL REGION-LEFT WEEK 91 93 MASS(ES) INGUINAL REGION-LEFT WEEK 91 94 MASS(ES) 95 INGUINAL REGION-LEFT WEEK 91 96 MASS(ES) 97 INGUINAL REGION-LEFT WEEK 91 98 MASS(ES) INGUINAL REGION-LEFT WEEK 91 99 MASS(ES) INGUINAL REGION-LEFT WEEK 91 15 X 15MM 25 X 25MM 30 X 25MM 40 X 25MM 50 X 30MM 60 X 40MM 70 X 40MM 70 X 50MM 1237 Covance 6329-183 3M T-6295 PAGE: 692 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C93183 C93186 F6 694 100 MASS(ES) TO 701 101 INGUINAL REGION-LEFT WEEK 91 708 102 MASS(ES) TO 715 103 INGUINAL REGION-LEFT WEEK 91 722 104 MASS(ES) TO 729 105 INGUINAL REGION-LEFT WEEK 91 736 106 MASS(ES) TO 736 106 INGUINAL REGION-LEFT WEEK 91 F6 309 45 TO 316 46 MASS(ES) VENTRAL-CERVICAL WEEK 45 323 47 TO 330 48 MASS(ES) VENTRAL-CERVICAL WEEK 45 337 49 MASS(ES) VENTRAL-CERVICAL WEEK 45 344 50 MASS(ES) VENTRAL-CERVICAL WEEK 45 80 X 50MM 90 X 70MM 110 X 80MM 125 X 100MM 20 X 15MM 25 X 25MM 30 X 25MM 35 X 30MM 1238 Covance 6329-183 3M T-6295 PAGE: 693 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 51 MASS(ES) 55 VENTRAL-CERVICAL WEEK 45 56 MASS(ES) 57 VENTRAL-CERVICAL WEEK 45 58 MASS(ES) 60 VENTRAL-CERVICAL WEEK 45 61 MASS(ES) 63 VENTRAL-CERVICAL WEEK 45 64 MASS(ES) 70 VENTRAL-CERVICAL WEEK 45 71 MASS(ES) VENTRAL-CERVICAL WEEK 45 72 MASS(ES) VENTRAL-CERVICAL WEEK 45 73 MASS(ES) VENTRAL-CERVICAL WEEK 45 40 X 35MM 40 X 40MM 50 X 50MM 60 X 60MM 70 X 70MM 80 X 70MM 80 X 80MM 95 X 80MM 1239 Covance 6329-183 3M T-6295 PAGE: 694 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C93186 C93187 F6 512 74 TO 520 75 MASS(ES) VENTRAL-CERVICAL WEEK 45 F6 708 102 MASS(ES) TO 725 104 AXILLARY REGION-RIGHT WEEK 102 561 81 TO 575 83 MASS(ES) INGUINAL REGION-LEFT WEEK 81 582 84 MASS(ES) INGUINAL REGION-LEFT WEEK 81 589 85 TO 596 86 MASS(ES) INGUINAL REGION-LEFT WEEK 81 603 87 TO 645 93 MASS(ES) INGUINAL REGION-LEFT WEEK 81 652 94 MASS(ES) TO 705 101 INGUINAL REGION-LEFT WEEK 81 708 102 MASS(ES) TO 725 104 INGUINAL REGION-LEFT WEEK 81 100 X 110MM 10 X 10MM 15 X 15MM 20 X 20MM 30 X 30MM 45 X 40MM 50 X 45MM 65 X 60MM 1240 Covance 6329-183 3M T-6295 PAGE: 695 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C93187 C93188 F6 701 101 MASS(ES) TO 705 101 INGUINAL REGION-RIGHT WEEK 101 F6 484 70 TO 505 73 MASS(ES) AXILLARY REGION-LEFT WEEK 70 512 74 TO 519 75 MASS(ES) AXILLARY REGION-LEFT WEEK 70 526 76 TO 540 78 MASS(ES) AXILLARY REGION-LEFT WEEK 70 547 79 TO 582 84 MASS(ES) AXILLARY REGION-LEFT WEEK 70 589 85 TO 596 86 MASS(ES) AXILLARY REGION-LEFT WEEK 70 603 87 TO 631 91 MASS(ES) AXILLARY REGION-LEFT WEEK 70 638 92 TO 659 95 MASS(ES) AXILLARY REGION-LEFT WEEK 70 10 X 10MM 25 X 30MM 40 X 40MM 40 X 50MM 55 X 60MM 60 X 65MM 65 X 70MM 75 X 80MM 1241 Covance 6329-183 3M T-6295 PAGE: 696 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C93188 C93192 F6 666 96 MASS(ES) AXILLARY REGION-LEFT WEEK 70 673 97 MASS(ES) TO 694 100 AXILLARY REGION-LEFT WEEK 70 701 101 MASS(ES) AXILLARY REGION-LEFT WEEK 70 708 102 MASS(ES) TO 715 103 AXILLARY REGION-LEFT WEEK 70 722 104 MASS(ES) TO 734 105 AXILLARY REGION-LEFT WEEK 70 F6 631 91 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 91 638 92 TO 659 95 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 91 666 96 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 91 90 X 85MM 95 X 90MM 80 X 85MM 95 X 95MM 100 X 100MM 25 X 25MM 30 X 30MM 45 X 30MM 1242 Covance 6329-183 3M T-6295 PAGE: 697 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS CATEGORY KEYWORD WK QUALIFIER FREE-TEXT COMMENT 97 MASS(ES) 98 VENTRAL-ABDOMINAL-RIGHT WEEK 91 99 MASS(ES) 101 VENTRAL-ABDOMINAL-RIGHT WEEK 91 102 MASS(ES) 105 VENTRAL-ABDOMINAL-RIGHT WEEK 91 106 MASS(ES) 106 VENTRAL-ABDOMINAL-RIGHT WEEK 91 82 MASS(ES) 86 INGUINAL REGION-RIGHT WEEK 81 87 MASS(ES) 92 VENTRAL-ABDOMINAL-LEFT WEEK 82 81 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 81 100 MASS(ES) 106 AXILLARY REGION-LEFT WEEK 99 50 X 40MM 50 X 50MM 55 X 70MM 70 X 75MM 10 X 10MM 10 X 10MM 10 X 10MM 10 X 10MM 1243 Covance 6329-183 3M T-6295 PAGE: 698 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT F6 645 93 MASS(ES) TO 701 101 INGUINAL REGION-LEFT WEEK 93 708 102 MASS(ES) TO 722 104 INGUINAL REGION-LEFT WEEK 93 729 105 MASS(ES) TO 736 106 INGUINAL REGION-LEFT WEEK 93 659 95 TO 680 98 MASS(ES) INGUINAL REGION-LEFT WEEK 95 687 99 MASS(ES) TO 701 101 INGUINAL REGION-LEFT WEEK 95 708 102 MASS(ES) TO 722 104 INGUINAL REGION-LEFT WEEK 95 729 105 MASS(ES) INGUINAL REGION-LEFT WEEK 95 736 106 MASS(ES) TO 736 106 INGUINAL REGION-LEFT WEEK 95 10 X 10MM 10 X 20MM 20 X 20MM 10 X 10MM POSTERIOR 15 X 15MM POSTERIOR 20 X 30MM POSTERIOR 30 X 40MM POSTERIOR 40 X 40MM 1244 Covance 6329-183 3M T-6295 PAGE: 699 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C93198 C93203 F6 694 100 MASS(ES) TO 736 106 AXILLARY REGION-RIGHT WEEK 94 F6 533 77 TO 561 81 MASS(ES) AXILLARY REGION-LEFT WEEK 76 568 82 TO 603 87 MASS(ES) AXILLARY REGION-LEFT WEEK 76 CO CO CO CO 610 TO 624 90 MASS(ES) AXILLARY REGION-LEFT WEEK 76 589 85 TO 603 87 MASS(ES) INGUINAL REGION-LEFT WEEK 81 610 TO 624 90 MASS(ES) INGUINAL REGION-LEFT WEEK 81 477 69 TO 505 73 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 69 512 74 TO 519 75 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 69 10 X 10MM 10 X 10MM 15 X 15MM 15 X 25MM 20 X 20MM 25 X 30MM 10 X 10MM 20 X 20MM 1245 Covance 6329-183 3M T-6295 PAGE: 700 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 76 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 69 77 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 69 62 MASS(ES) 67 VENTRAL-ABDOMINAL-RIGHT WEEK 62 68 MASS(ES) 69 VENTRAL-ABDOMINAL-RIGHT WEEK 62 70 MASS(ES) 73 VENTRAL-ABDOMINAL-RIGHT WEEK 62 74 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 62 75 MASS(ES) 83 VENTRAL-ABDOMINAL-RIGHT WEEK 62 84 MASS(ES) 86 VENTRAL-ABDOMINAL-RIGHT WEEK 62 30 X 30MM 20 X 25MM 10 X 10MM 15 X 15MM 20 X 20MM 30 X 30MM 25 X 30MM 15 X 20MM 1246 Covance 6329-183 3M T-6295 PAGE: 701 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 81 MASS(ES) 84 VENTRAL-THORACIC-LEFT WEEK 81 66 MASS(ES) 67 VENTRAL-ABDOMINAL-RIGHT WEEK 66 68 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 66 69 MASS(ES) 71 VENTRAL-ABDOMINAL-RIGHT WEEK 66 72 MASS(ES) 75 VENTRAL-ABDOMINAL-RIGHT WEEK 66 76 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 66 77 MASS(ES) 79 VENTRAL-ABDOMINAL-RIGHT WEEK 66 80 MASS(ES) 85 VENTRAL-ABDOMINAL-RIGHT WEEK 66 20 X 20MM 20 X 20MM 25 X 25MM 30 X 30MM 35 X 30MM 35 X 40MM 45 X 45MM 60 X 60MM 1247 Covance 6329-183 3M T-6295 PAGE: 702 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER C93204 C93207 F6 596 86 TO 603 87 610 TO 631 91 638 92 TO 642 92 F6 71 11 TO 78 12 85 13 TO 155 23 162 24 TO 169 25 176 26 TO 204 30 211 31 CO CO MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 66 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 66 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 66 MASS(ES) VENTRAL-THORACIC-RIGHT WEEK 10 MASS(ES) VENTRAL-THORACIC-RIGHT WEEK 10 MASS(ES) VENTRAL-THORACIC-RIGHT WEEK 10 MASS(ES) VENTRAL-THORACIC-RIGHT WEEK 10 MASS(ES) VENTRAL-THORACIC-RIGHT WEEK 10 FREE-TEXT COMMENT 70 X 60MM 70 X 70MM 70 X 80MM 15 X 20MM 20 X 25MM 25 X 25MM 35 X 35MM 45 X 45MM 1248 Covance 6329-183 3M T-6295 PAGE: 703 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C93207 C93210 F6 218 32 TO 239 35 MASS(ES) VENTRAL-THORACIC-RIGHT WEEK 10 246 36 TO 260 38 MASS(ES) VENTRAL-THORACIC-RIGHT WEEK 10 267 39 TO 281 41 MASS(ES) VENTRAL-THORACIC-RIGHT WEEK 10 F6 652 94 MASS(ES) INGUINAL REGION-LEFT WEEK 94 659 95 MASS(ES) INGUINAL REGION-LEFT WEEK 94 666 96 MASS(ES) TO 705 101 INGUINAL REGION-LEFT WEEK 94 708 102 MASS(ES) INGUINAL REGION-LEFT WEEK 94 715 103 MASS(ES) TO 734 105 INGUINAL REGION-LEFT WEEK 94 40 X 40MM 45 X 50MM 55 X 55MM 25 X 25MM 25 X 30MM 35 X 30MM 40 X 40MM 50 X 50MM 1249 Covance 6329-183 3M T-6295 PAGE: 704 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C93210 C93216 CO CO CO CO F6 610 TO 624 90 MASS(ES) INGUINAL REGION-RIGHT WEEK 88 631 91 TO 645 93 MASS(ES) INGUINAL REGION-RIGHT WEEK 88 652 94 TO 666 96 MASS(ES) INGUINAL REGION-RIGHT WEEK 88 673 97 MASS(ES) TO 734 105 INGUINAL REGION-RIGHT WEEK 88 F6 561 81 TO 582 84 MASS(ES) AXILLARY REGION-RIGHT WEEK 81 589 85 TO 603 87 MASS(ES) AXILLARY REGION-RIGHT WEEK 81 610 TO 624 90 MASS(ES) AXILLARY REGION-RIGHT WEEK 81 631 91 TO 638 92 MASS(ES) AXILLARY REGION-RIGHT WEEK 81 15 X 15MM 20 X 20MM 30 X 25MM 30 X 35MM 10 X 10MM 20 X 20MM 20 X 25MM 25 X3 0MM 1250 Covance 6329-183 3M T-6295 PAGE: 705 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS CATEGORY KEYWORD WK QUALIFIER FREE-TEXT COMMENT 93 MASS(ES) AXILLARY REGION-RIGHT WEEK 81 94 MASS(ES) 95 AXILLARY REGION-RIGHT WEEK 81 96 MASS(ES) 101 AXILLARY REGION-RIGHT WEEK 81 102 MASS(ES) 106 AXILLARY REGION-RIGHT WEEK 81 95 MASS(ES) 99 AXILLARY REGION-RIGHT WEEK 93 100 MASS(ES) 103 AXILLARY REGION-RIGHT WEEK 93 104 MASS(ES) 106 AXILLARY REGION-RIGHT WEEK 93 88 MASS(ES) 90 EAR-RIGHT WEEK 61 30 X 30MM 40 X 40MM 50 X 40MM 50 X 60MM 10 X 10MM 15 X 15MM 20 X 25MM 10 X 10MM 1251 Covance 6329-183 3M T-6295 PAGE: 706 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C93216 F6 701 101 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 101 708 102 MASS(ES) TO 736 106 VENTRAL-ABDOMINAL-LEFT WEEK 101 547 79 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 79 561 81 TO 582 84 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 81 589 85 TO 603 87 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 81 610 88 TO 631 91 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 81 638 92 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 81 673 97 MASS(ES) TO 701 101 VENTRAL-ABDOMINAL-RIGHT WEEK 96 15 X 15MM 25 X 25MM 10 X 10MM 10 X 10MM 20 X 20MM 20 X 30MM 20 X 20MM 15 X 20MM 1252 Covance 6329-183 3M T-6295 PAGE: 707 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS CATEGORY KEYWORD WK QUALIFIER FREE-TEXT COMMENT 102 MASS(ES) 103 VENTRAL-ABDOMINAL-RIGHT WEEK 96 104 MASS(ES) 105 VENTRAL-ABDOMINAL-RIGHT WEEK 96 106 MASS(ES) 106 VENTRAL-ABDOMINAL-RIGHT WEEK 96 53 MASS(ES) 57 AXILLARY REGION-LEFT WEEK 50 58 MASS(ES) 83 AXILLARY REGION-LEFT WEEK 50 84 MASS(ES) 86 AXILLARY REGION-LEFT WEEK 50 87 MASS(ES) 90 AXILLARY REGION-LEFT WEEK 50 85 MASS(ES) 102 VENTRAL-THORACIC-RIGHT WEEK 85 20 X 30MM 20 X 40MM 40 X 40MM 10 X 10MM 20 X 20MM 15 X 15MM 20 X 20MM 10 X 10MM 1253 Covance 6329-183 3M T-6295 PAGE: 708 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C93220 F6 673 97 MASS(ES) TO 694 100 VENTRAL-ABDOMINAL WEEK 97 701 101 MASS(ES) VENTRAL-ABDOMINAL WEEK 97 708 102 MASS(ES) TO 715 103 VENTRAL-ABDOMINAL WEEK 97 722 104 MASS(ES) TO 729 105 VENTRAL-ABDOMINAL WEEK 97 736 106 MASS(ES) TO 736 106 VENTRAL-ABDOMINAL WEEK 97 20 X 20MM 30 X 20MM 45 X 40MM 60 X 50MM 70 X 60MM Covance 6329-183 3M T-6295 PAGE: 709 1254 COVAH^ THE DEVELOPMENT SERVICES COMPANY Sponsor: 3M St. Paul, Minnesota FINAL REPORT Study Title: 104-Week Dietary Chronic Toxicity and Carcinogenicity Study with Perfluorooctane Sulfonic Acid Potassium Salt (PFOS; T-6295) in Rats Author: Peter J. Thomford, PhD Study Completion Date: January 2, 2002 Testing Facility: Covance Laboratories Inc. 3301 Kinsman Boulevard Madison, Wisconsin 53704-2595 Laboratory Study Identification: Covance 6329-183 Sponsor Study Identification: 3M T-6295 Volume IV of IX Page 1255 o f 4068 Covance 6329-183 3M T-6295 VOLUME IV OF IX CONTENTS Page APPENDIX 3................................................................................................................ 1257 Individual Body Weight Data (g).............................................................................. 1258 APPENDIX 4 ................................................................................................................ 1345 Individual Food Consumption Data (g).....................................................................1346 Individual Test Material Consumption Data (mg/kg/day)........................................ 1432 APPENDIX 5................................................................................................................ 1512 Individual Clinical Hematology Data........................................................................1513 Individual Clinical Chemistry Data...........................................................................1585 Individual Clinical Urinalysis Data...........................................................................1652 Individual Urine Chemistry Data.............................................................................. 1724 1256 APPENDIX 3 Individual Body Weight Data (g) Covance 6329-183 3M T-6295 1257 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 GROUP: MALE 1 - 0 PPM C92501 193 257 315 369 415 456 479 514 C92502 184 235 275 316 349 371 385 406 C92503 180 237 292 347 380 415 435 464 C92504 167 221 272 317 343 368 391 432 C92505 154 201 234 273 298 328 352 379 C92506 188 247 303 355 C92507 189 251 311 360 391 425 439 463 C92508 156 213 253 300 325 355 372 405 C92509 172 231 281 330 369 397 416 446 C92510 218 276 324 372 404 435 453 479 C92511 179 239 288 334 367 399 423 456 C92512 175 232 277 326 352 383 405 434 C92513 149 206 251 301 335 370 389 413 C92514 220 269 321 374 415 446 474 504 C92515 171 213 260 301 340 363 381 408 C92516 174 225 276 322 354 381 393 414 C92517 194 259 319 378 430 473 492 529 C92518 185 244 299 339 380 404 430 459 C92519 165 222 263 307 338 362 386 413 C92520 174 227 283 326 355 383 408 434 C92521 168 220 268 318 339 378 400 417 C92522 189 243 292 340 368 389 421 433 C92523 146 202 247 303 336 370 393 421 C92524 208 276 332 384 C92525 158 217 267 324 366 398 425 451 C92526 178 240 291 348 C92527 183 239 285 340 375 399 416 449 C92528 155 206 244 286 318 346 363 387 C92529 171 222 263 295 C92530 207 267 307 350 380 407 431 456 C92531 155 210 267 312 341 375 393 420 Covance 6329-183 3M T-6295 PAGE: 1 WEEK WEEK 910 535 571 419 434 479 501 456 473 395 411 475 491 415 428 458 476 494 507 469 482 453 469 434 447 541 544 424 438 414 436 543 569 481 505 433 442 457 476 438 452 456 463 440 459 473 486 466 489 406 418 474 486 440 456 1258 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 GROUP: MALE 1 - 0 PPM C92532 182 248 311 376 417 462 484 526 C92533 204 278 342 393 430 465 488 520 C92534 174 232 273 316 357 384 405 429 C92535 172 235 284 332 372 399 426 455 C92536 191 248 294 342 375 412 419 445 C92537 183 253 314 375 422 465 493 527 C92538 158 217 274 325 364 400 424 451 C92539 207 271 323 366 410 441 458 499 C92540 197 256 309 350 390 425 443 474 C92541 183 251 306 365 406 445 463 501 C92542 192 251 308 357 385 422 433 463 C92543 215 274 323 361 385 419 437 462 C92544 187 250 306 353 390 412 423 455 C92545 198 255 294 337 367 396 413 443 C92546 195 250 298 345 C92547 208 271 328 384 432 469 496 528 C92548 173 231 277 320 341 369 400 420 C92549 158 207 252 299 327 360 374 403 C92550 148 198 253 305 352 396 396 440 C92551 160 217 267 302 339 361 380 409 C92552 178 234 287 328 366 401 417 451 C92553 141 186 222 266 275 302 312 335 C92554 164 219 266 304 335 357 379 404 C92555 176 225 271 305 332 362 395 422 C92556 196 247 300 348 381 403 414 440 C92557 165 221 269 314 353 382 403 430 C92558 177 238 297 353 392 431 457 492 C92559 198 257 303 339 367 398 416 442 C92560 197 256 303 346 373 401 418 451 C92561 154 208 260 308 351 389 409 431 C92562 184 246 300 337 371 399 414 439 Covance 6329-183 3M T-6295 PAGE: 2 WEEK WEEK 910 552 565 537 557 445 459 480 494 449 468 555 573 471 488 522 550 492 501 521 541 479 493 478 481 399 423 454 473 555 569 433 445 410 420 471 495 424 433 469 486 341 353 417 430 440 458 456 472 447 466 507 527 456 466 460 476 452 474 449 463 1259 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 GROUP: MALE 1 - 0 PPM C92563 183 243 293 340 374 411 434 457 C92564 173 229 279 324 364 405 433 462 C92565 174 230 280 320 346 379 405 437 C92566 168 230 286 340 382 426 429 467 C92567 180 241 292 338 378 412 432 464 C92568 142 196 249 295 332 369 398 428 C92569 200 256 300 338 374 403 430 457 C92570 191 259 310 352 385 420 434 463 GROUP: MALE 2 - 0.5 PPM C92571 168 217 272 322 358 388 414 436 C92572 160 222 278 328 365 392 427 446 C92573 190 255 318 373 406 460 495 508 C92574 195 256 304 358 C92575 184 239 283 310 C92576 213 267 317 364 399 433 455 466 C92577 169 231 289 344 382 412 422 472 C92578 179 239 292 332 366 394 431 459 C92579 209 266 321 373 407 450 479 503 C92580 169 232 285 332 367 401 434 453 C92581 187 253 317 368 410 440 466 491 C92582 145 202 257 298 319 343 366 379 C92583 185 238 292 322 353 381 402 417 C92584 159 209 247 278 308 332 351 364 C92585 204 268 316 353 387 425 455 475 C92586 161 208 262 307 331 372 405 427 C92587 178 240 297 344 383 422 452 493 C92588 184 235 285 322 359 390 419 442 C92589 179 242 303 355 394 432 459 486 C92590 178 244 309 361 406 437 470 495 Covance 6329-183 3M T-6295 PAGE: 3 WEEK WEEK 910 477 495 485 502 458 476 489 513 481 496 452 471 475 488 480 488 453 480 462 480 527 554 481 504 489 511 469 482 526 543 470 494 504 526 397 414 425 446 376 394 491 509 446 468 508 530 455 471 498 517 506 530 1260 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEE1K WEE2K WEEK 3 WEEK 4 WEEK 5 WEE6K WEEK 7 WEE8K GROUP: MALE 2 - 0.5 PPM C92591 176 229 275 311 351 373 392 409 C92592 143 208 268 316 369 395 427 456 C92593 195 251 310 342 389 406 428 450 C92594 181 238 293 334 379 402 427 441 C92595 180 238 293 332 370 398 423 449 C92596 199 253 297 330 362 391 417 433 C92597 188 240 287 320 355 382 397 412 C92598 171 230 283 329 366 395 425 447 C92599 219 285 341 382 420 462 488 510 C92600 176 228 275 312 351 382 406 429 C92601 149 206 259 305 349 376 403 423 C92602 169 224 267 302 341 365 381 399 C92603 196 259 312 356 396 421 443 462 200C92604 252 304 343 381 405 432 458 202C92605 263 311 348 388 420 451 471 C92606 152 209 260 305 343 375 395 420 210C92607 157 219 283 336 381 415 438 468 C92608 272 320 371 406 454 470 495 C92609 179 239 298 334 373 403 428 448 C92610 187 249 301 336 C92611 190 245 300 333 373 396 415 432 C92612 159 215 268 316 359 392 414 441 C92613 171 230 294 332 C92614 197 260 317 353 396 426 450 477 C92615 168 231 284 327 361 391 424 448 C92616 144 189 235 266 295 323 342 361 C92617 184 249 309 359 409 447 483 513 C92618 165 225 284 338 C92619 174 230 279 312 347 374 401 414 C92620 171 217 261 295 329 358 380 394 C92621 193 254 304 338 380 413 439 460 Covance 6329-183 3M T-6295 PAGE: 4 WEEK 9 WE10EK 424 441 474 497 454 470 456 475 453 468 445 465 422 438 465 479 517 545 440 457 436 450 403 418 472 490 472 489 486 508 436 455 488 507 505 526 457 473 444 462 455 481 490 514 459 475 374 389 528 552 421 439 407 418 474 497 1261 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 GROUP: MALE 2 - 0 .5 PPM C92622 145 210 269 322 364 399 431 452 C92623 162 214 259 297 326 359 380 402 C92624 202 255 297 331 351 379 401 418 C92625 159 223 288 351 393 443 476 499 C92626 173 236 287 335 379 414 440 463 C92627 191 257 312 354 392 427 453 474 C92628 194 258 312 351 394 422 457 474 C92629 149 215 274 321 364 400 430 454 C92630 193 259 315 359 395 427 455 475 GROUP: MALE 3 - 2 PPM C92631 186 240 290 323 361 384 403 425 C92632 220 290 344 396 435 469 496 528 C92633 159 213 257 270 312 349 380 411 C92634 147 199 248 288 328 357 377 397 C92635 178 245 303 349 387 418 437 467 C92636 197 263 308 342 373 401 413 437 C92637 169 218 257 287 319 346 361 380 C92638 184 248 292 343 389 435 466 503 C92639 169 219 259 301 335 370 401 425 C92640 191 248 286 319 344 366 377 393 C92641 179 233 284 323 367 398 425 452 C92642 201 254 304 344 386 413 432 458 C92643 151 211 256 304 339 359 374 403 C92644 185 245 295 333 365 382 394 406 C92645 176 238 281 319 361 390 415 432 C92646 226 282 329 369 C92647 188 244 291 328 358 384 396 419 C92648 176 228 269 304 335 355 368 386 C92649 158 203 246 279 309 336 353 375 Covance 6329-183 3M T-6295 PAGE: 5 WEEK 9 WEEK 10 466 473 386 416 438 451 516 545 488 510 494 515 494 518 473 497 484 501 435 456 553 577 426 449 413 430 471 497 440 466 384 400 516 555 438 464 397 410 459 488 459 486 407 430 408 420 442 463 421 454 385 405 375 397 1262 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 GROUP: MALE 3 - 2 PPM C92650 158 215 271 315 C92651 196 260 312 355 394 428 443 470 C92652 156 213 265 313 C92653 173 230 284 330 375 404 423 453 C92654 171 233 282 335 376 400 421 459 C92655 157 218 277 328 374 407 438 474 C92656 162 220 273 322 376 415 435 474 C92657 176 234 286 333 367 402 421 447 C92658 192 249 297 344 383 411 437 464 C92659 206 269 319 358 402 430 449 477 C92660 195 257 299 339 375 395 419 434 C92661 205 260 302 341 377 404 419 439 C92662 164 214 261 299 335 362 379 404 C92663 171 233 292 350 401 428 443 479 C92664 197 256 310 349 392 416 435 462 C92665 187 252 309 356 402 433 457 487 C92666 199 257 310 358 399 435 455 477 C92667 181 243 298 344 392 425 452 484 C92668 190 243 289 332 C92669 167 223 268 307 345 376 398 419 C92670 165 224 274 316 351 381 389 415 C92671 146 200 252 306 347 376 402 400 C92672 153 209 252 295 333 346 380 411 C92673 200 262 307 351 385 423 433 459 C92674 174 237 277 310 339 368 393 425 C92675 189 250 299 340 373 404 413 437 C92676 197 259 293 332 352 380 404 425 C92677 171 229 273 317 351 384 411 430 C92678 140 195 243 293 C92679 180 237 285 331 374 407 425 451 C92680 209 269 323 365 403 439 462 490 Covance 6329-183 3M T-6295 PAGE: 6 WEEK WEEK 910 480 509 464 494 456 483 489 514 476 514 451 481 470 499 480 508 436 454 448 464 410 435 483 507 469 490 503 525 483 506 490 523 422 447 417 429 417 446 424 443 469 489 431 456 433 459 430 449 430 452 464 487 500 524 1263 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 GROUP: MALE 3 - 2 PPM C92681 164 218 270 315 353 382 410 433 C92682 171 224 280 325 357 385 407 425 C92683 180 241 287 336 374 407 426 449 C92684 183 237 277 315 343 374 393 414 C92685 160 219 265 318 361 398 424 455 C92686 193 250 300 342 372 402 426 457 C92687 206 264 305 341 388 421 447 478 C92688 176 228 268 308 344 375 391 414 C92689 155 216 267 319 360 395 419 445 C92690 201 276 343 405 458 486 516 552 GROUP: MALE 4 - 5 PPM C92691 163 214 277 314 349 378 408 432 C92692 146 199 258 313 350 383 415 448 C92693 175 227 285 327 365 401 426 451 C92694 194 249 296 336 366 402 427 458 C92695 202 260 314 355 383 414 439 462 C92696 204 261 308 349 379 412 436 455 C92697 183 242 293 334 366 399 428 447 C92698 188 254 304 345 389 425 450 471 C92699 162 214 251 287 317 346 372 374 C92700 171 219 257 288 312 333 352 362 C92701 191 249 297 339 368 404 422 447 C92702 157 216 267 312 349 379 404 431 C92703 150 209 264 313 356 391 425 448 C92704 177 236 282 329 367 401 425 454 C92705 168 222 272 311 350 383 400 431 C92706 174 235 289 327 362 398 424 445 C92707 181 235 284 324 350 377 396 416 C92708 177 239 294 344 370 386 406 425 Covance 6329-183 3M T-6295 PAGE: 7 WEEK WEEK 910 441 464 438 453 457 480 420 436 468 493 466 475 490 511 424 448 445 450 567 587 445 457 463 474 468 480 482 498 468 476 472 481 468 485 488 498 396 412 376 385 463 484 443 457 473 489 475 489 448 458 466 482 427 442 435 450 1264 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 GROUP: MALE 4 - 5 PPM C92709 199 255 297 325 354 387 413 443 C92710 162 217 267 311 349 386 418 442 C92711 179 229 282 319 350 385 414 440 C92712 192 257 321 361 398 435 467 482 C92713 217 280 345 393 431 458 475 501 C92714 176 233 300 346 379 407 432 455 C92715 194 249 310 349 C92716 211 269 336 380 414 452 481 515 C92717 164 224 286 329 369 398 424 442 C92718 156 212 266 305 C92719 139 190 251 299 333 362 391 415 C92720 189 248 321 363 397 434 460 480 C92721 186 236 292 333 326 353 378 392 C92722 194 248 304 347 380 417 439 459 C92723 188 242 307 352 393 423 430 476 C92724 206 264 330 374 413 448 481 503 C92725 165 217 274 312 330 365 386 408 C92726 168 224 284 331 370 403 431 453 C92727 182 233 286 330 357 391 419 441 C92728 162 227 284 348 401 437 478 507 C92729 201 263 331 384 428 463 493 516 C92730 158 215 268 308 339 374 397 419 C92731 199 260 309 336 C92732 147 205 264 312 343 376 406 431 C92733 173 231 280 317 346 366 388 401 C92734 178 241 293 333 C92735 217 273 321 354 391 421 444 464 C92736 175 229 281 318 352 381 400 422 C92737 183 240 290 328 363 391 415 431 C92738 141 198 254 302 335 368 394 420 C92739 164 215 259 292 319 345 358 379 Covance 6329-183 3M T-6295 PAGE: 8 WEEK WEEK 910 456 463 456 472 460 473 503 516 517 527 480 499 534 547 462 475 428 436 498 514 412 425 473 490 492 513 523 527 416 428 474 484 459 470 531 544 532 542 439 448 446 464 410 429 479 492 439 454 450 467 438 463 397 409 1265 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 GROUP: MALE 4 - 5 PPM C92740 173 229 290 337 370 398 425 455 C92741 198 252 301 339 378 410 438 454 C92742 178 245 308 364 404 448 478 508 C92743 159 224 289 340 386 428 458 486 C92744 154 211 266 318 C92745 198 252 304 356 389 429 454 480 C92746 209 272 332 381 409 452 474 502 C92747 184 234 283 321 356 384 418 434 C92748 193 252 302 350 387 415 452 480 C92749 154 207 254 289 323 355 378 401 C92750 189 248 301 340 374 406 429 443 GROUP: MALE 5 - 20 PPM C92751 164 219 275 317 364 400 421 454 C92752 167 223 263 295 C92753 206 267 320 358 406 435 444 474 C92754 178 220 267 299 329 353 373 389 C92755 146 200 249 294 325 350 372 394 C92756 191 254 312 354 403 439 467 488 C92757 164 207 251 286 320 349 373 388 C92758 149 207 259 303 343 376 400 420 C92759 197 249 301 343 C92760 196 252 303 344 382 407 424 455 C92761 183 237 289 323 358 383 406 417 C92762 183 238 288 324 367 397 426 446 C92763 182 246 307 355 395 431 463 488 C92764 197 264 327 369 398 435 456 470 C92765 185 240 295 335 378 409 434 453 C92766 171 230 282 318 356 385 403 420 C92767 168 233 293 345 388 411 450 475 Covance 6329-183 3M T-6295 PAGE: 9 WEEK WEEK 910 479 489 468 480 535 551 516 530 499 516 519 533 453 464 497 515 417 430 463 470 468 490 494 511 394 411 406 435 500 522 406 416 436 449 464 476 431 441 458 468 511 529 494 513 470 487 432 449 503 525 1266 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 GROUP: MALE 5 - 20 PPM C92768 209 269 331 377 417 455 484 504 C92769 182 240 297 339 374 405 428 456 C92770 217 283 339 374 410 441 462 486 C92771 165 226 280 328 364 396 427 451 C92772 175 231 288 337 366 398 423 437 C92773 180 235 282 319 351 375 386 406 C92774 166 226 281 317 354 389 416 443 C92775 155 220 277 332 372 409 437 464 C92776 189 253 308 356 392 418 432 455 C92777 151 205 254 291 330 349 370 390 C92778 202 265 321 362 407 429 446 466 C92779 160 217 258 295 C92780 159 211 250 280 310 336 356 374 C92781 183 241 288 320 362 385 407 423 C92782 138 196 247 295 334 363 382 407 C92783 200 266 319 360 402 436 464 482 C92784 183 242 288 334 361 394 415 425 C92785 190 239 290 324 359 387 411 428 C92786 172 232 280 317 356 383 412 427 C92787 206 260 308 342 383 406 425 438 C92788 181 248 310 354 387 418 445 460 C92789 155 211 262 303 338 369 387 408 C92790 150 216 266 312 352 384 409 434 C92791 179 229 272 307 346 372 393 405 C92792 186 243 297 337 380 409 427 447 C92793 192 247 290 325 C92794 187 237 279 309 341 367 385 400 C92795 168 223 269 300 337 363 384 415 C92796 171 220 260 290 324 350 377 390 C92797 201 249 297 324 356 376 395 409 C92798 162 223 273 315 Covance 6329-183 3M T-6295 PAGE: 10 WEEK 9 WEEK 10 509 531 476 494 507 522 464 484 454 476 419 433 456 475 482 495 477 491 404 420 480 491 392 408 433 455 428 452 494 517 430 455 439 457 440 459 452 471 474 502 433 447 447 471 417 438 463 487 413 433 434 452 404 422 422 445 1267 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 GROUP: MALE 5 - 20 PPM C92799 181 239 282 317 357 393 431 452 C92800 184 243 293 327 361 390 409 429 C92801 210 266 318 359 407 441 468 486 C92802 173 231 281 314 350 377 405 423 C92803 200 257 311 341 384 416 446 459 C92804 169 222 263 299 336 367 391 399 C92805 194 259 310 352 382 418 448 469 C92806 164 223 283 326 374 402 426 448 C92807 152 210 261 300 333 366 398 421 C92808 150 202 250 292 328 355 374 391 C92809 170 226 282 319 358 389 411 431 C92810 204 257 304 334 368 393 412 426 C92811 167 225 282 324 351 395 415 440 C92812 182 240 293 326 365 395 428 449 C92813 183 250 300 334 374 400 423 446 C92814 146 196 242 276 305 325 343 354 C92815 204 249 283 302 334 361 344 370 C92816 141 194 237 276 307 342 366 385 C92817 187 248 300 347 393 432 461 481 C92818 189 245 291 319 357 382 402 414 C92819 155 204 244 274 301 327 352 371 C92820 186 229 270 299 331 358 380 396 GROUP: MALE 6 - 20 PPM C92821 144 193 244 291 323 360 389 413 C92822 165 214 266 305 340 369 391 414 C92823 181 237 293 333 368 400 418 432 C92824 200 255 298 335 373 401 429 453 C92825 183 248 310 361 408 444 476 498 C92826 181 224 272 307 340 371 387 406 Covance 6329-183 3M T-6295 PAGE: 11 WEEK 9 WEEK 10 472 491 438 462 509 533 433 451 470 492 418 441 482 504 416 433 439 456 405 422 447 471 441 454 446 478 455 481 453 470 359 372 379 409 396 415 500 532 422 453 381 398 398 419 417 437 419 438 444 460 460 484 513 535 412 435 1268 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 GROUP: MALE 6 - 20 PPM C92827 192 236 284 322 353 385 408 427 C92828 184 247 300 342 380 412 437 460 C92829 187 241 293 332 376 418 440 463 C92830 158 210 259 294 330 361 370 390 C92831 202 258 318 353 389 418 438 457 C92832 153 200 257 299 334 360 385 400 C92833 164 220 279 331 367 403 427 458 C92834 177 225 280 326 364 392 412 431 C92835 168 221 273 301 332 362 378 400 C92836 213 268 329 370 405 436 449 477 C92837 175 233 301 347 395 433 457 478 C92838 172 226 282 319 359 384 407 424 C92839 187 254 329 392 441 490 521 554 C92840 187 236 282 315 340 366 384 403 C92841 185 227 281 314 350 376 389 407 C92842 213 275 340 378 421 443 466 490 C92843 210 273 331 371 407 434 458 478 C92844 152 199 248 281 306 329 347 363 C92845 193 237 285 320 351 373 392 411 C92846 166 224 281 321 364 396 423 451 C92847 170 227 298 349 397 436 465 496 C92848 135 178 221 252 283 306 328 352 C92849 173 226 287 332 368 400 424 449 C92850 205 250 296 321 359 379 396 411 C92851 173 222 283 325 368 403 419 445 C92852 156 207 263 299 339 370 388 411 C92853 192 242 292 325 357 383 403 422 C92854 193 254 317 352 386 420 430 460 C92855 175 229 284 323 359 387 406 370 C92856 151 200 262 301 339 367 388 413 C92857 189 247 308 355 395 426 447 476 Covance 6329-183 3M T-6295 PAGE: 12 WEEK 9 WEEK 10 435 456 472 493 480 507 404 425 475 488 415 426 478 497 442 463 409 431 446 476 494 509 435 458 559 586 407 419 414 431 495 511 490 512 368 379 418 441 468 489 512 538 358 383 454 477 420 435 457 475 412 439 434 454 460 475 405 431 422 437 487 506 1269 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 GROUP: MALE 6 - 20 PPM C92858 164 218 275 327 371 404 430 456 C92859 191 240 295 333 365 399 426 451 C92860 158 205 256 296 337 356 379 398 GROUP: FEMALE 1 - 0 PPM C92861 176 204 239 251 C92862 134 153 174 191 198 213 217 226 C92863 147 179 202 218 232 247 244 264 C92864 156 193 223 245 C92865 151 189 211 226 241 249 257 273 C92866 170 183 218 231 239 245 249 253 C92867 153 186 209 235 265 263 265 290 C92868 147 170 194 208 237 241 247 255 C92869 140 157 181 199 209 215 216 224 C92870 141 168 192 209 215 231 228 243 C92871 161 192 218 229 250 254 264 273 C92872 141 168 181 193 200 210 206 215 C92873 149 164 173 195 211 213 207 221 C92874 146 186 206 217 240 251 255 261 C92875 142 171 184 194 214 215 232 242 C92876 144 175 192 210 220 233 234 246 C92877 150 174 196 214 224 223 228 234 C92878 143 178 207 216 225 227 241 248 C92879 167 198 222 240 249 260 269 285 C92880 157 185 205 212 219 234 245 254 C92881 154 184 210 221 236 249 255 267 C92882 146 168 190 205 206 216 222 230 C92883 171 210 241 258 273 284 290 312 C92884 154 190 210 219 234 241 236 254 C92885 163 194 217 235 245 256 263 274 Covance 6329-183 3M T-6295 PAGE: 13 WEEK 9 WEEK 10 470 496 458 468 406 423 229 235 267 277 262 276 268 285 286 302 266 281 223 237 246 256 278 278 218 219 224 225 267 277 241 255 247 254 244 251 249 244 289 306 254 261 270 278 232 243 313 325 257 267 279 294 1270 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 GROUP: FEMALE 1 - 0 PPM C92886 149 180 199 201 209 227 231 235 C92887 159 188 214 233 238 266 263 258 C92888 165 191 232 254 269 282 302 304 C92889 141 167 190 202 218 231 235 240 C92890 176 216 246 267 275 291 297 307 C92891 144 173 199 212 218 230 240 245 C92892 161 198 226 249 278 302 311 329 C92893 151 176 201 215 216 233 235 244 C92894 162 183 206 217 227 227 232 241 C92895 165 190 202 231 246 255 252 271 C92896 140 173 199 217 226 244 249 262 C92897 142 167 188 197 204 214 223 237 C92898 146 181 202 218 236 248 253 262 C92899 163 200 225 258 279 298 307 330 C92900 168 195 223 240 250 263 274 288 C92901 143 171 190 206 225 235 235 254 C92902 168 200 232 254 272 289 301 334 C92903 182 232 257 283 299 300 306 329 C92904 145 173 184 202 211 227 225 244 C92905 143 175 193 212 224 235 238 250 C92906 138 168 193 210 212 227 232 240 C92907 143 169 189 210 227 229 242 249 C92908 152 182 207 211 238 249 251 263 C92909 156 178 199 215 229 234 243 262 C92910 156 182 211 227 250 269 272 296 C92911 146 167 184 189 212 210 218 233 C92912 155 184 211 232 240 264 267 286 C92913 161 190 217 236 242 253 257 271 C92914 149 170 190 192 C92915 150 163 191 209 231 239 249 268 C92916 166 196 236 264 278 289 304 319 Covance 6329-183 3M T-6295 PAGE: 14 WEEK 9 WEEK 10 234 252 259 267 317 324 242 254 301 322 246 256 323 344 237 250 249 248 269 272 258 275 234 244 270 280 323 351 294 303 245 259 348 362 332 342 241 255 253 261 241 251 251 266 272 286 262 268 292 310 227 248 286 296 275 286 259 262 331 328 1271 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 GROUP: FEMALE 1 - 0 PPM C92917 164 204 226 264 273 290 302 306 C92918 167 196 214 229 240 253 256 265 C92919 153 187 219 265 C92920 139 163 178 198 213 217 227 237 C92921 165 202 217 239 256 271 285 301 C92922 155 187 224 228 235 253 250 275 C92923 133 161 175 196 214 222 225 239 C92924 158 187 205 226 248 259 269 283 C92925 156 191 218 243 252 275 282 302 C92926 168 195 219 228 C92927 159 180 205 213 234 241 255 260 C92928 163 195 215 221 250 259 263 266 C92929 152 174 202 216 229 238 247 260 C92930 172 199 246 270 297 305 324 330 GROUP: FEMALE 2 - 0.5 PPM C92931 156 191 220 240 260 273 282 292 C92932 150 184 207 221 228 242 246 259 C92933 151 173 192 211 231 252 268 277 C92934 148 170 189 200 215 221 231 244 C92935 150 176 198 219 241 252 259 270 C92936 138 169 195 210 214 227 232 247 C92937 150 180 195 217 229 239 250 257 C92938 156 180 200 213 243 252 258 280 C92939 152 166 179 190 206 223 216 234 C92940 144 165 187 202 216 224 228 238 C92941 156 184 207 229 C92942 158 194 218 237 245 260 271 288 C92943 162 189 214 239 253 279 282 298 C92944 173 204 236 252 283 304 309 320 Covance 6329-183 3M T-6295 PAGE: 15 WEEK 9 WEEK 10 324 339 267 271 235 247 309 321 272 289 242 241 287 294 303 315 261 280 274 290 253 266 335 352 309 316 256 269 293 315 247 250 279 295 245 259 262 273 283 287 233 240 242 255 287 305 302 305 332 347 1272 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 GROUP: FEMALE 2 - 0.5 PPM C92945 146 161 193 207 C92946 140 157 184 194 201 207 225 235 C92947 150 179 194 221 236 256 263 269 C92948 161 175 212 235 252 283 273 299 C92949 175 216 245 289 291 311 346 357 C92950 156 180 193 221 C92951 153 174 189 195 202 216 222 238 C92952 141 163 181 195 218 223 230 248 C92953 147 175 189 210 217 229 237 246 C92954 141 163 195 209 229 244 245 261 C92955 157 188 210 226 245 259 257 280 C92956 165 196 216 246 259 271 273 289 C92957 148 177 198 209 216 229 226 246 C92958 146 161 181 188 205 218 218 224 C92959 167 201 231 245 255 272 269 286 C92960 165 192 235 255 270 272 285 291 C92961 181 226 247 263 302 313 319 332 C92962 157 178 205 210 222 234 232 241 C92963 150 169 187 194 205 217 220 224 C92964 165 189 208 231 226 246 242 250 C92965 141 175 199 211 234 242 247 256 C92966 142 167 186 198 210 223 226 235 C92967 144 173 202 224 241 256 263 274 C92968 151 186 215 241 242 263 274 283 C92969 149 167 183 197 C92970 161 187 218 235 260 273 280 289 C92971 148 155 185 200 215 224 226 236 C92972 145 169 201 215 235 242 250 254 C92973 143 153 184 199 214 223 235 243 C92974 153 175 186 209 223 231 232 245 C92975 150 175 197 216 222 242 242 260 Covance 6329-183 3M T-6295 PAGE: 16 WEEK 9 WEEK 10 242 251 279 293 315 319 359 387 233 248 248 260 245 256 262 270 276 301 298 311 246 248 233 244 295 313 302 294 331 342 249 255 231 245 254 268 261 271 234 241 276 290 288 301 305 328 232 245 267 269 241 254 248 256 254 265 1273 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEE1K WEE2K WEEK 3 WEEK 4 WEEK 5 WEE6K WEEK 7 WEE8K GROUP: FEMALE 2 - 0.5 PPM 221C92976 178 204 233 253 264 277 279 296 C92977 140 165 191 216 248 265 275 C92978 170 208 237 270 278 304 305 324 C92979 147 173 191 209 229 242 246 259 C92980 147 171 193 217 231 253 259 267 C92981 C92982 C92983 C92984 162 153 140 168 192 176 180 198 220227 224141 248 220251 222 241 237 259 259 261 275 263 283 282 278 304 295 C92985 163 193 216 242 261 266 284 294 222C92986 144 165 191 205 229 241 252 220C92987 164 186 235 245 248 262 269 200C92988 154 168 215 230 238 246 261 222C92989 151 176 203 225 238 247 251 C92990 171 196 226 235 242 254 263 274 GROUP: FEMALE 3 - 2 PPM C92991 C92992 142 158 155 185 210825 202 212 214 231 216 241 228 244 241 254 C92993 156 182 203 218 227 246 260 270 C92994 153 170 184 204 224 234 246 244 C92995 154 177 204 215 241 248 250 267 C92996 157 172 205 221 C92997 160 177 197 206 225 238 237 247 C92998 159 176 198 207 225 243 242 266 C92999 156 189 215 232 248 262 256 280 C93000 176 193 215 234 247 252 257 266 212C93001 141 163 185 196 228 232 235 221 221C93002 158 179 203 239 236 245 212C93003 163 183 218 251 268 269 283 Covance 6329-183 3M T-6295 PAGE: 17 WEEK 9 WE10EK 303 310 275 287 334 339 262 270 278 285 283 289 293 301 306 314 306 308 250 257 272 275 263 263 258 261 275 269 245 249 260 259 272 284 256 264 264 245 262 270 280 285 287 260 276 243 251 252 247 290 308 1274 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 GROUP: FEMALE 3 - 2 PPM C93004 150 170 194 206 223 226 235 261 C93005 160 184 197 218 225 230 241 247 C93006 160 175 206 212 C93007 154 178 196 208 228 239 240 247 C93008 173 192 218 240 C93009 150 165 186 201 218 232 245 255 C93010 152 183 207 225 243 250 255 264 C93011 156 186 210 234 257 263 269 272 C93012 167 192 228 236 271 290 289 317 C93013 139 148 177 190 198 214 219 238 C93014 165 200 221 247 270 296 287 303 C93015 165 187 215 237 248 260 268 284 C93016 135 144 160 166 191 199 212 220 C93017 152 162 184 197 218 224 235 248 C93018 163 180 209 233 237 250 260 273 C93019 163 188 213 234 248 263 269 279 C93020 156 167 203 228 239 248 258 266 C93021 141 171 191 220 236 251 259 276 C93022 164 187 210 230 246 262 261 285 C93023 144 155 174 196 208 214 227 239 C93024 163 193 206 234 248 257 257 268 C93025 174 198 220 250 255 270 286 300 C93026 141 161 188 206 221 240 254 261 C93027 156 179 203 224 231 246 256 264 C93028 156 178 200 219 224 237 247 261 C93029 146 162 191 200 223 228 235 244 C93030 146 169 193 204 225 238 242 238 C93031 143 162 184 203 C93032 152 171 192 217 218 234 233 246 C93033 168 188 196 224 226 243 248 264 C93034 168 195 207 238 237 259 269 269 Covance 6329-183 3M T-6295 PAGE: 18 WEEK 9 WEEK 10 252 268 251 248 250 258 258 268 267 271 280 286 308 319 231 233 295 308 286 291 225 226 248 260 273 278 289 292 270 280 274 279 281 295 241 243 266 269 303 315 260 273 261 271 260 261 239 234 248 268 250 259 265 269 270 278 1275 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 GROUP: FEMALE 3 - 2 PPM C93035 151 179 207 226 238 257 266 273 C93036 157 173 191 216 222 237 246 253 C93037 143 168 188 216 236 244 258 265 C93038 140 156 170 192 197 215 216 218 C93039 144 160 188 202 228 244 254 272 C93040 134 151 162 180 188 205 212 213 C93041 158 182 196 219 235 240 254 263 C93042 163 194 216 244 261 276 272 294 C93043 148 163 195 208 226 248 245 269 C93044 154 168 195 208 229 232 239 253 C93045 162 200 221 243 C93046 164 201 237 268 278 291 300 303 C93047 171 200 231 249 282 299 298 316 C93048 140 157 177 182 203 211 220 218 C93049 151 169 195 210 216 227 237 241 C93050 163 178 200 220 234 244 250 258 GROUP: FEMALE 4 - 5 PPM C93051 150 165 196 196 223 222 234 234 C93052 151 178 202 211 231 246 250 260 C93053 151 169 194 202 215 229 228 248 C93054 150 160 178 187 205 219 224 239 C93055 156 185 208 223 234 251 256 272 C93056 145 179 208 222 231 248 256 268 C93057 140 162 186 198 218 231 230 241 C93058 169 210 233 257 C93059 150 181 203 223 233 250 254 269 C93060 161 185 198 219 238 249 247 275 C93061 159 184 200 226 238 249 252 269 C93062 146 165 189 198 215 229 234 244 Covance 6329-183 3M T-6295 PAGE: 19 WEEK 9 WEEK 10 274 287 259 267 264 276 222 236 274 281 223 230 267 273 291 292 267 273 256 257 309 325 314 326 223 234 239 244 260 265 251 250 263 277 248 264 238 249 269 286 265 278 236 245 267 274 276 280 267 278 253 264 1276 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 GROUP: FEMALE 4 - 5 PPM C93063 156 179 213 213 245 256 254 263 C93064 144 169 191 202 217 227 230 235 C93065 128 150 169 172 190 197 197 203 C93066 162 192 216 232 234 253 251 270 C93067 162 190 199 231 C93068 158 174 200 220 222 237 246 254 C93069 168 207 237 267 275 288 296 303 C93070 164 192 219 236 C93071 146 167 182 195 201 200 210 221 C93072 143 173 198 213 219 230 240 246 C93073 157 179 204 214 222 240 247 259 C93074 161 192 232 245 253 263 261 279 C93075 155 174 192 205 C93076 146 172 192 214 225 255 241 257 C93077 164 197 220 242 255 261 262 284 C93078 150 171 195 205 215 232 246 254 C93079 159 188 212 225 254 250 267 286 C93080 146 171 207 218 238 251 253 265 C93081 143 167 177 198 205 223 233 240 C93082 169 196 208 230 254 264 265 289 C93083 155 181 208 221 231 251 251 265 C93084 144 167 193 206 C93085 155 182 209 213 234 245 251 262 C93086 164 190 212 236 249 260 267 281 C93087 135 163 186 189 204 214 220 230 C93088 154 177 200 214 232 237 242 253 C93089 173 210 233 253 259 267 278 286 C93090 154 183 205 217 226 234 237 241 C93091 168 198 221 236 261 258 277 289 C93092 152 176 196 212 216 231 233 248 C93093 159 194 216 219 255 269 277 295 Covance 6329-183 3M T-6295 PAGE: 20 WEEK 9 WEEK 10 260 264 237 245 210 221 257 265 254 264 309 326 219 233 251 259 258 272 276 290 264 265 283 296 247 261 310 299 259 266 244 248 291 294 265 277 261 273 281 283 235 236 249 254 290 296 250 250 278 302 248 262 302 325 1277 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 GROUP: FEMALE 4 - 5 PPM C93094 132 159 188 202 215 230 257 257 C93095 143 177 200 212 225 233 238 249 C93096 145 176 209 236 248 258 274 294 C93097 154 166 183 188 209 221 230 246 C93098 143 168 190 206 218 224 231 242 C93099 153 176 207 221 235 248 251 255 C93100 170 196 217 231 242 244 251 265 C93101 156 170 196 204 219 218 233 245 C93102 165 200 230 241 260 269 284 290 C93103 151 184 200 225 223 252 249 263 C93104 171 199 229 245 267 285 304 328 C93105 158 188 227 233 261 278 263 278 C93106 158 178 201 212 227 228 236 253 C93107 143 172 198 210 228 243 249 260 C93108 148 166 191 197 213 219 227 244 C93109 150 174 200 207 228 245 244 254 C93110 173 193 234 261 288 307 316 341 GROUP: FEMALE 5 - 20 PPM C93111 160 192 224 257 261 275 277 295 C93112 175 191 221 237 240 259 265 264 C93113 139 155 161 177 194 199 200 217 C93114 162 194 219 222 C93115 151 159 178 189 193 203 203 210 C93116 146 180 201 218 216 225 231 240 C93117 138 167 179 206 215 219 228 242 C93118 152 164 185 199 218 218 214 229 C93119 148 171 183 187 204 205 202 215 C93120 144 182 195 214 216 225 227 236 C93121 132 155 166 180 187 191 197 212 Covance 6329-183 3M T-6295 PAGE: 21 WEEK 9 WEEK 10 254 271 246 261 295 308 247 256 247 256 260 274 263 284 244 263 296 310 273 278 337 361 277 292 247 253 268 280 244 264 257 272 343 361 295 306 264 265 220 219 209 218 233 245 236 256 223 233 206 205 235 233 206 213 1278 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 GROUP: FEMALE 5 - 20 PPM C93122 167 194 229 232 261 272 266 273 C93123 166 192 212 228 C93124 152 183 200 209 222 236 236 244 C93125 140 152 169 182 193 198 199 213 C93126 139 159 172 191 196 211 204 217 C93127 156 184 203 207 227 240 234 242 C93128 161 175 201 202 219 222 227 243 C93129 144 168 192 216 236 239 245 250 C93130 159 182 209 227 241 241 245 261 C93131 160 187 215 229 239 250 256 264 C93132 158 192 214 230 238 243 247 252 C93133 152 184 204 205 220 225 223 234 C93134 180 214 223 234 246 255 265 268 C93135 144 180 201 225 230 247 244 260 C93136 161 190 208 208 225 238 240 242 C93137 144 160 188 201 205 212 219 233 C93138 163 184 207 220 227 234 239 252 C93139 139 166 179 199 207 212 206 214 C93140 146 175 199 229 227 246 245 255 C93141 150 182 209 231 240 259 263 273 C93142 171 198 210 238 C93143 169 191 222 238 238 257 266 276 C93144 137 149 176 184 C93145 135 160 173 194 203 210 213 217 C93146 147 172 187 201 196 204 207 220 C93147 146 174 196 209 226 231 236 246 C93148 146 179 205 222 226 234 249 266 C93149 145 159 185 197 210 223 224 234 C93150 171 196 229 235 248 263 258 266 C93151 157 188 207 223 225 234 239 249 C93152 160 189 207 227 235 241 246 261 Covance 6329-183 3M T-6295 PAGE: 22 WEEK 9 WEEK 10 282 293 235 243 215 210 217 226 242 250 238 250 251 263 257 272 270 275 241 256 230 240 274 277 252 265 244 246 230 229 247 262 213 223 260 266 271 278 277 274 214 226 211 225 244 254 266 287 235 244 266 273 245 255 260 268 1279 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 GROUP: FEMALE 5 - 20 PPM C93153 168 194 204 226 230 236 233 246 C93154 150 179 205 220 226 243 246 252 C93155 151 168 196 220 220 221 222 244 C93156 139 164 180 201 201 211 218 227 C93157 161 181 192 221 223 229 229 244 C93158 147 175 200 212 225 234 235 240 C93159 151 168 189 204 C93160 151 183 215 239 247 262 276 286 C93161 144 177 200 226 233 241 251 257 C93162 175 206 236 246 243 258 262 279 C93163 146 168 191 211 220 223 226 237 C93164 160 170 195 192 209 208 220 221 C93165 147 177 193 210 216 229 230 243 C93166 167 197 226 237 267 272 266 291 C93167 160 172 213 233 237 257 252 268 C93168 144 157 189 201 220 231 229 241 C93169 157 177 197 209 229 234 237 242 C93170 156 180 202 220 228 251 254 261 C93171 156 181 191 220 226 238 247 253 C93172 157 180 199 216 232 236 241 258 C93173 148 174 197 219 220 224 232 242 C93174 155 169 188 200 202 221 231 236 C93175 144 173 198 208 217 225 227 240 C93176 130 160 183 199 215 227 229 238 C93177 158 172 189 198 211 220 222 233 C93178 149 176 199 213 231 233 236 244 C93179 145 171 191 205 209 219 226 232 C93180 174 201 229 250 257 270 274 283 Covance 6329-183 3M T-6295 PAGE: 23 WEEK 9 WEEK 10 241 236 251 264 231 236 223 236 243 255 251 260 286 301 257 273 277 271 234 239 224 237 241 250 297 300 276 270 234 243 237 251 257 268 256 262 254 246 237 257 235 239 234 251 236 247 229 235 241 250 229 240 281 292 1280 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 GROUP: FEMALE 6 - 20 PPM C93181 151 170 187 203 208 220 233 248 C93182 150 171 192 205 211 217 222 238 C93183 161 184 197 227 239 248 262 275 C93184 134 159 168 194 206 218 215 229 C93185 163 181 204 222 229 240 245 253 C93186 156 162 182 200 210 201 215 221 C93187 157 180 200 204 232 234 242 251 C93188 160 188 208 231 237 245 246 260 C93189 147 171 192 197 213 216 222 231 C93190 152 181 199 220 229 243 244 257 C93191 167 191 231 244 260 276 278 298 C93192 161 179 198 203 218 220 225 227 C93193 157 173 199 217 229 233 240 246 C93194 173 202 220 246 264 271 274 289 C93195 170 192 218 237 244 252 262 274 C93196 135 152 174 179 199 200 211 216 C93197 151 167 181 188 201 205 210 213 C93198 157 179 197 215 236 239 247 250 C93199 161 189 205 221 226 233 235 245 C93200 151 167 189 198 212 224 230 241 C93201 133 158 183 202 209 222 228 237 C93202 140 153 194 207 228 234 236 248 C93203 156 183 208 220 245 241 258 266 C93204 156 191 201 226 236 234 242 254 C93205 179 205 221 249 254 265 269 276 C93206 148 159 176 191 195 213 216 227 C93207 139 161 184 195 210 222 222 228 C93208 163 187 197 225 223 238 234 246 C93209 168 190 201 202 224 240 228 238 C93210 161 193 210 213 239 245 257 257 C93211 145 165 173 198 203 216 217 227 Covance 6329-183 3M T-6295 PAGE: 24 WEEK 9 WEEK 10 240 249 231 250 276 285 227 231 250 261 217 213 252 256 263 272 228 240 251 262 306 306 231 232 245 240 285 297 266 270 213 224 215 225 245 257 243 248 231 241 230 238 249 239 262 271 246 245 273 282 220 232 229 238 241 249 238 244 263 266 223 231 1281 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 GROUP: FEMALE 6 - 20 PPM C93212 146 172 204 205 231 237 246 263 C93213 157 186 202 223 234 236 245 253 C93214 148 166 193 198 213 222 226 242 C93215 145 169 198 219 233 241 249 256 C93216 142 169 188 207 213 227 236 242 C93217 162 193 220 240 259 270 267 286 C93218 136 162 181 201 207 215 221 235 C93219 181 214 242 266 276 285 275 297 C93220 166 192 207 229 247 250 253 257 Covance 6329-183 3M T-6295 PAGE: 25 WEEK 9 WEEK 10 259 262 251 257 231 244 255 264 238 252 283 291 230 236 293 299 267 276 1282 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 11 WEEK 12 WEEK 13 WEEK 14 WEEK 15 WEEK 16 WEEK 17 WEEK 21 GROUP: MALE 1 - 0 PPM C92501 591 603 615 641 639 657 667 698 C92502 452 460 463 469 474 484 486 509 C92503 512 511 517 523 529 547 549 560 C92504 494 475 466 474 476 487 491 523 C92505 422 419 432 439 447 460 468 488 C92507 506 507 522 526 532 539 541 557 C92508 443 454 460 469 471 481 492 517 C92509 499 502 515 523 C92510 528 531 541 555 568 572 575 602 C92511 500 515 526 541 C92512 486 498 505 520 525 547 552 585 C92513 466 477 489 496 506 520 524 544 C92514 577 593 603 620 640 658 668 719 C92515 457 470 479 485 495 515 516 541 C92516 459 464 469 476 479 492 496 505 C92517 587 599 605 615 628 644 656 694 C92518 530 534 553 562 586 595 601 641 C92519 461 465 476 488 490 500 507 523 C92520 492 508 516 526 533 545 549 568 C92521 476 481 491 502 C92522 484 490 495 508 506 519 519 531 C92523 477 496 503 520 521 539 545 576 C92525 508 523 540 554 560 581 592 613 C92527 503 517 528 534 535 556 556 592 C92528 432 447 454 464 C92530 496 507 521 529 535 547 561 585 C92531 474 480 486 501 512 524 532 555 C92532 581 594 600 620 C92533 573 589 592 612 611 627 643 658 C92534 473 482 484 489 500 509 516 531 C92535 503 514 512 519 524 547 559 578 C92536 480 491 497 515 526 541 548 581 Covance 6329-183 3M T-6295 PAGE: 26 WEEK 25 725 522 592 555 504 571 540 616 607 576 753 562 530 731 668 547 589 548 619 645 609 608 588 675 559 603 610 WEEK 29 762 548 630 572 530 590 574 627 625 609 806 588 564 785 706 570 616 581 655 670 645 646 628 719 601 642 661 1283 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 11 WEEK 12 WEEK 13 WEEK 14 WEEK 15 WEEK 16 WEEK 17 WEEK 21 GROUP: MALE 1 - 0 PPM C92537 594 612 632 647 654 665 685 724 C92538 506 521 534 551 571 581 594 631 C92539 566 582 591 607 620 635 643 685 C92540 523 536 545 557 562 576 583 613 C92541 566 583 593 602 610 622 640 667 C92542 508 512 509 517 518 526 534 559 C92543 500 505 509 517 531 542 539 558 C92544 420 468 495 505 521 529 538 560 C92545 482 497 502 513 518 538 546 575 C92547 591 606 614 632 632 648 644 668 C92548 465 469 480 489 498 512 510 537 C92549 438 441 445 449 458 463 468 479 C92550 521 535 548 559 571 591 602 644 C92551 455 458 471 475 480 496 499 527 C92552 507 516 524 541 553 567 573 600 C92553 362 365 372 371 376 376 383 378 C92554 449 457 461 465 470 484 487 511 C92555 475 489 502 526 536 553 562 610 C92556 489 496 508 517 524 544 553 576 C92557 483 494 502 514 520 536 536 562 C92558 555 557 562 576 582 593 597 626 C92559 484 489 490 500 505 517 524 539 C92560 492 506 512 524 526 541 546 572 C92561 500 512 527 534 544 553 555 588 C92562 483 493 499 518 512 528 536 560 C92563 514 522 534 540 542 561 572 594 C92564 524 537 561 576 589 606 620 662 C92565 498 517 521 531 534 542 550 571 C92566 534 544 553 566 559 576 577 552 C92567 510 514 516 534 538 551 548 583 C92568 489 503 514 515 524 535 548 567 Covance 6329-183 3M T-6295 PAGE: 27 WEEK 25 WEEK 29 767 808 668 733 721 764 662 694 701 742 584 607 579 606 589 616 609 639 689 716 575 630 509 538 686 730 542 556 631 687 375 368 534 565 661 700 610 662 598 623 646 696 568 599 602 655 618 650 588 619 618 656 705 760 620 672 611 637 588 627 1284 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 11 WEEK 12 WEEK 13 WEEK 14 WEEK 15 WEEK 16 WEEK 17 WEEK 21 GROUP: MALE 1 - 0 PPM C92569 511 507 515 520 528 541 559 572 C92570 506 527 531 543 551 561 573 598 GROUP: MALE 2 - 0.5 PPM C92571 503 522 529 548 558 570 570 602 C92572 500 511 514 537 543 552 557 596 C92573 580 592 601 620 629 642 652 694 C92576 528 540 539 558 564 577 585 620 C92577 538 553 563 585 598 615 631 683 C92578 509 524 526 549 562 565 575 615 C92579 565 583 583 599 603 615 624 656 C92580 513 525 524 540 542 554 562 598 C92581 550 557 560 572 583 596 593 622 C92582 432 445 443 452 456 462 463 471 C92583 459 467 469 480 477 486 491 519 C92584 408 419 422 435 436 444 448 465 C92585 522 535 531 553 558 573 578 622 C92586 491 499 512 533 551 568 575 629 C92587 553 566 573 596 610 626 634 667 C92588 483 490 499 509 519 525 528 548 C92589 536 547 555 570 577 586 591 616 C92590 556 563 567 592 602 614 619 651 C92591 459 470 475 494 498 509 520 556 C92592 517 525 520 531 538 545 551 579 C92593 488 495 498 506 C92594 495 497 498 505 513 520 532 559 C92595 482 490 487 495 501 517 521 533 C92596 488 496 503 521 524 536 542 567 C92597 451 454 459 474 478 487 497 520 C92598 497 509 513 529 525 534 537 568 Covance 6329-183 3M T-6295 PAGE: 28 WEEK 25 WEEK 29 596 629 619 638 619 646 618 663 723 777 631 680 722 752 650 695 680 714 618 653 648 685 502 542 533 562 478 503 646 682 660 698 708 755 568 595 634 685 673 698 581 621 611 654 581 614 547 587 602 648 540 571 591 616 1285 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 11 WEEK 12 WEEK 13 WEEK 14 WEEK 15 WEEK 16 WEEK 17 WEEK 21 GROUP: MALE 2 - 0.5 PPM C92599 559 569 568 587 592 605 603 642 C92600 471 480 487 499 C92601 471 471 478 495 500 510 524 551 C92602 434 437 433 445 445 460 461 491 C92603 513 519 532 543 545 557 555 C92604 510 518 532 550 557 575 580 627 C92605 528 541 551 570 576 592 608 647 C92606 483 495 504 519 529 540 546 592 C92607 528 541 552 571 576 588 600 626 C92608 557 574 587 604 579 576 578 623 C92609 487 490 493 504 509 518 528 562 C92611 474 483 487 497 505 510 512 540 C92612 506 516 523 538 534 551 562 615 C92614 537 539 548 563 574 582 587 617 C92615 498 507 520 539 548 565 566 612 C92616 406 411 416 428 C92617 570 583 601 614 630 644 654 704 C92619 453 460 466 483 482 495 496 527 C92620 424 429 433 449 454 459 468 496 C92621 512 530 539 555 C92622 504 515 528 538 543 555 564 592 C92623 434 447 458 468 474 482 494 524 C92624 468 479 487 511 513 514 529 567 C92625 567 578 595 610 616 637 647 689 C92626 529 539 548 561 572 560 566 598 C92627 529 545 554 560 C92628 536 547 548 564 573 586 589 613 C92629 518 528 543 560 571 585 593 649 C92630 520 531 535 555 559 571 580 623 Covance 6329-183 3M T-6295 PAGE: 29 WEEK 25 WEEK 29 663 693 583 606 508 517 659 698 695 735 621 643 660 673 667 710 600 629 563 580 586 670 654 675 658 696 741 766 537 559 514 538 634 662 543 578 610 650 728 756 639 645 653 674 680 738 654 672 1286 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 11 WEEK 12 WEEK 13 WEEK 14 WEEK 15 WEEK 16 WEEK 17 WEEK 21 GROUP: MALE 3 - 2 PPM C92631 455 473 490 494 505 510 515 536 C92632 583 573 616 627 644 641 663 703 C92633 458 477 492 503 508 517 528 561 C92634 435 448 462 472 485 497 496 522 C92635 496 509 514 525 534 542 550 581 C92636 469 485 503 514 517 533 545 581 C92637 404 414 430 432 441 450 452 477 C92638 572 598 611 622 639 648 661 717 C92639 469 488 499 512 525 531 540 562 C92640 409 409 414 416 C92641 496 477 502 525 544 561 580 606 C92642 494 510 531 541 547 560 566 583 C92643 435 450 467 472 480 483 495 507 C92644 420 441 451 458 459 468 480 491 C92645 469 480 499 510 C92647 461 468 481 488 494 509 519 538 C92648 413 416 429 438 447 458 466 486 C92649 403 414 427 434 440 451 457 484 C92651 506 523 538 549 548 561 575 588 C92653 501 512 532 541 560 568 579 617 C92654 477 500 516 525 531 546 548 552 C92655 530 554 569 575 587 606 607 634 C92656 491 536 561 575 580 593 601 625 C92657 485 506 514 520 530 545 551 586 C92658 501 521 537 553 560 577 581 623 C92659 509 529 548 562 570 585 589 627 C92660 451 468 473 480 483 488 492 506 C92661 464 477 486 497 508 528 534 561 C92662 438 444 448 459 C92663 521 526 542 552 560 577 579 615 C92664 495 515 533 538 540 550 558 592 Covance 6329-183 3M T-6295 PAGE: 30 WEEK 25 WEEK 29 568 584 742 791 602 624 569 600 621 657 614 639 498 521 775 842 609 649 649 664 615 654 533 554 514 537 568 590 513 542 511 533 626 665 654 696 581 616 667 693 659 691 615 639 640 671 665 694 527 534 597 627 665 711 621 646 1287 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 11 WEEK 12 WEEK 13 WEEK 14 WEEK 15 WEEK 16 WEEK 17 WEEK 21 GROUP: MALE 3 - 2 PPM C92665 543 555 568 578 590 606 614 639 C92666 509 531 551 557 563 571 581 590 C92667 530 556 577 592 604 614 620 666 C92669 452 471 489 497 505 515 529 567 C92670 432 449 461 463 469 474 482 507 C92671 462 477 487 499 512 522 527 559 C92672 454 478 496 508 515 528 539 568 C92673 501 516 524 537 540 552 559 594 C92674 470 485 498 513 526 544 551 597 C92675 460 479 479 487 496 506 516 539 C92676 453 463 477 482 C92677 461 469 479 473 486 491 501 542 C92679 499 510 526 539 546 560 575 618 C92680 533 553 568 576 577 589 596 642 C92681 473 492 506 516 524 533 547 570 C92682 465 479 490 499 507 518 533 570 C92683 492 507 519 526 537 544 552 593 C92684 436 450 454 457 C92685 508 517 534 538 555 570 579 619 C92686 488 494 507 517 524 527 539 576 C92687 522 552 567 581 596 607 619 658 C92688 457 467 480 488 492 499 501 530 C92689 457 469 468 478 491 503 511 545 C92690 603 612 630 642 652 668 677 727 GROUP: MALE 4 - 5 PPM C92691 472 487 486 496 506 526 532 566 C92692 487 512 517 529 534 547 547 581 C92693 498 513 514 526 541 554 561 581 C92694 517 530 529 532 546 560 572 603 Covance 6329-183 3M T-6295 PAGE: 31 WEEK 25 WEEK 29 680 721 620 653 694 704 592 628 516 538 581 608 602 628 618 634 633 663 558 577 552 582 640 672 652 676 600 629 594 626 622 662 652 683 600 630 685 715 543 563 576 595 736 773 595 620 592 622 603 633 616 651 1288 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 11 WEEK 12 WEEK 13 WEEK 14 WEEK 15 WEEK 16 WEEK 17 WEEK 21 GROUP: MALE 4 - 5 PPM C92695 494 504 506 513 521 533 537 570 C92696 494 508 513 517 530 549 551 563 C92697 495 507 518 532 540 555 559 575 C92698 522 534 546 566 579 597 600 643 C92699 428 440 450 463 470 485 491 483 C92700 394 399 398 412 413 426 428 443 C92701 482 506 516 534 546 549 555 589 C92702 473 478 484 491 499 510 518 538 C92703 513 528 538 546 561 574 589 625 C92704 508 525 533 546 556 571 583 605 C92705 477 493 499 511 524 541 542 570 C92706 500 518 523 540 552 566 571 594 C92707 449 467 469 471 475 489 498 523 C92708 462 478 482 496 503 513 520 541 C92709 466 480 478 489 496 515 525 554 C92710 497 509 516 527 545 559 563 584 C92711 492 501 509 520 519 537 549 578 C92712 533 549 548 561 578 595 602 641 C92713 545 554 563 576 C92714 526 542 551 567 C92716 569 570 580 595 601 622 633 667 C92717 493 501 517 534 545 559 567 600 C92719 459 467 474 491 C92720 532 548 549 562 577 591 599 626 C92721 438 447 457 467 480 489 492 513 C92722 473 504 518 536 C92723 526 544 558 572 583 597 603 634 C92724 538 549 561 581 603 614 625 655 C92725 449 456 460 469 478 484 488 528 C92726 498 512 517 525 528 539 552 558 C92727 484 490 498 509 515 527 542 577 Covance 6329-183 3M T-6295 PAGE: 32 WEEK 25 WEEK 29 582 603 567 575 592 611 665 688 521 530 448 460 607 642 555 568 657 678 625 666 592 624 630 670 536 556 557 566 571 593 600 613 598 624 679 719 682 711 624 659 641 673 528 554 663 687 681 718 541 559 574 605 620 648 1289 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 11 WEEK 12 WEEK 13 WEEK 14 WEEK 15 WEEK 16 WEEK 17 WEEK 21 GROUP: MALE 4 - 5 PPM C92728 567 587 596 611 C92729 554 566 581 596 621 635 641 683 C92730 467 483 483 491 505 523 530 553 C92732 477 486 495 507 525 533 546 572 C92733 442 448 446 458 473 486 492 513 C92735 507 513 521 531 541 554 565 575 C92736 470 477 488 504 518 537 545 557 C92737 481 490 494 505 521 537 543 565 C92738 471 492 495 508 520 529 532 545 C92739 424 436 439 447 460 472 476 488 C92740 504 509 521 529 543 553 566 578 C92741 499 511 508 527 533 546 560 578 C92742 567 587 604 623 639 655 663 680 C92743 545 565 575 591 612 631 638 672 C92745 536 557 567 590 605 624 636 678 C92746 548 575 578 592 596 613 622 646 C92747 479 488 498 511 522 543 545 551 C92748 530 544 558 570 569 585 604 615 C92749 440 450 458 465 486 504 510 522 C92750 484 495 499 513 527 546 552 547 GROUP: MALE 5 - 20 PPM C92751 506 515 524 539 547 564 577 613 C92753 496 499 501 514 514 529 535 567 C92754 420 426 431 441 440 448 449 459 C92755 449 461 463 479 491 504 509 538 C92756 531 541 552 573 584 602 603 630 C92757 431 426 432 447 451 465 467 489 C92758 464 477 485 500 506 520 518 542 C92760 486 494 500 513 522 528 527 546 Covance 6329-183 3M T-6295 PAGE: 33 WEEK 25 WEEK 29 716 764 591 628 599 639 537 574 598 622 587 629 595 630 560 591 521 545 597 625 600 618 713 751 706 741 721 756 665 706 570 602 638 664 544 573 594 629 647 681 559 556 467 499 569 595 648 679 514 538 559 576 554 582 1290 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 11 WEEK 12 WEEK 13 WEEK 14 WEEK 15 WEEK 16 WEEK 17 WEEK 21 GROUP: MALE 5 - 20 PPM C92761 453 454 459 471 481 486 482 498 C92762 481 486 492 506 509 518 520 533 C92763 548 565 575 589 612 624 629 676 C92764 515 527 534 546 556 558 565 582 C92765 498 512 516 526 C92766 453 461 461 477 486 497 494 516 C92767 545 569 578 600 599 622 637 680 C92768 535 552 548 562 568 581 576 609 C92769 504 517 528 545 553 569 564 610 C92770 533 554 563 570 578 584 583 598 C92771 495 506 514 528 545 554 547 582 C92772 483 496 500 513 522 533 540 563 C92773 435 451 456 457 455 462 455 463 C92774 488 498 510 526 533 541 543 579 C92775 509 526 542 549 561 569 579 598 C92776 501 522 530 545 559 568 562 581 C92777 424 439 446 455 C92778 505 521 530 537 549 557 560 580 C92780 413 420 428 439 451 465 462 471 C92781 461 469 473 484 495 506 504 517 C92782 458 467 476 490 500 515 516 540 C92783 527 537 544 559 569 580 589 614 C92784 463 475 489 497 503 514 523 531 C92785 469 479 483 495 507 517 524 520 C92786 470 483 494 503 502 518 523 557 C92787 482 488 499 509 517 526 528 551 C92788 515 525 539 552 561 570 565 572 C92789 459 466 479 449 C92790 480 491 497 518 528 541 535 543 C92791 444 451 458 470 481 484 493 503 C92792 498 498 509 526 529 538 544 560 Covance 6329-183 3M T-6295 PAGE: 34 WEEK 25 WEEK 29 504 516 553 578 724 777 599 621 545 575 733 799 624 653 629 651 616 649 610 653 588 610 467 491 604 616 630 668 599 624 595 623 498 515 537 563 557 597 635 680 556 587 537 561 579 613 572 590 589 609 563 582 525 546 591 604 1291 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 11 WEEK 12 WEEK 13 WEEK 14 WEEK 15 WEEK 16 WEEK 17 WEEK 21 GROUP: MALE 5 - 20 PPM C92794 444 456 460 474 479 488 489 505 C92795 462 472 480 489 502 515 521 543 C92796 427 430 437 448 453 462 466 480 C92797 453 465 479 487 493 505 512 537 C92799 503 521 535 552 C92800 470 481 484 498 501 509 511 513 C92801 538 560 571 590 596 612 615 643 C92802 456 470 476 483 493 501 508 523 C92803 495 502 514 520 524 528 536 544 C92804 448 461 467 483 490 498 510 520 C92805 510 527 543 555 563 575 581 611 C92806 456 473 496 518 524 538 535 555 C92807 470 479 491 499 513 530 536 548 C92808 433 443 446 457 462 473 478 483 C92809 481 494 503 509 520 527 529 546 C92810 465 472 481 494 503 514 518 533 C92811 486 496 509 513 526 539 551 581 C92812 491 504 513 523 C92813 479 483 495 506 516 519 526 541 C92814 376 375 375 374 377 377 389 392 C92815 415 419 430 442 452 450 460 462 C92816 428 436 445 456 463 472 474 493 C92817 547 550 568 581 592 600 613 657 C92818 460 470 475 482 491 495 499 522 C92819 412 416 414 416 426 430 432 457 C92820 398 405 415 415 427 442 442 455 GROUP: MALE 6 - 20 PPM C92821 447 456 471 481 485 491 488 508 C92822 440 448 455 465 480 488 491 520 Covance 6329-183 3M T-6295 PAGE: 35 WEEK 25 WEEK 29 531 549 571 599 499 519 561 590 525 556 677 722 551 589 558 547 541 560 629 661 571 604 569 599 493 527 561 588 561 586 609 648 562 584 406 436 479 507 514 542 702 744 537 566 476 508 466 489 475 518 542 580 1292 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 11 WEEK 12 WEEK 13 WEEK 14 WEEK 15 WEEK 16 WEEK 17 WEEK 21 GROUP: MALE 6 - 20 PPM C92823 469 481 487 499 510 522 531 544 C92824 487 498 510 519 533 539 543 561 C92825 551 557 566 583 597 611 610 664 C92826 441 450 463 475 485 488 498 507 C92827 459 465 475 492 498 501 507 524 C92828 504 518 513 516 527 533 532 563 C92829 521 528 545 552 570 580 583 611 C92830 437 442 447 464 476 487 493 492 C92831 494 506 521 512 524 528 538 577 C92832 430 441 450 462 478 480 476 502 C92833 508 520 529 542 557 570 577 606 C92834 473 484 488 498 510 513 517 544 C92835 433 444 443 454 459 477 481 515 C92836 500 514 526 533 542 547 544 564 C92837 520 522 535 545 546 556 555 584 C92838 462 469 476 491 506 511 510 534 C92839 602 619 634 656 663 670 685 732 C92840 423 432 438 453 462 471 474 496 C92841 434 444 450 443 459 468 468 484 C92842 517 530 540 550 561 559 564 581 C92843 528 532 538 542 545 549 558 587 C92844 379 384 385 390 391 398 405 419 C92845 442 454 457 467 476 483 486 497 C92846 496 513 531 541 538 551 563 582 C92847 550 566 582 593 605 622 622 641 C92848 388 402 414 428 438 441 445 464 C92849 482 488 498 510 521 524 530 555 C92850 438 436 436 444 442 448 452 474 C92851 483 488 495 507 512 525 535 546 C92852 442 449 451 460 462 464 472 479 C92853 449 462 463 473 477 487 490 517 Covance 6329-183 3M T-6295 PAGE: 36 WEEK 25 WEEK 29 550 576 584 621 705 752 539 569 547 581 583 605 645 680 512 541 606 629 520 535 642 677 565 592 534 565 580 609 610 641 559 586 775 811 516 538 497 516 601 624 613 648 431 450 521 546 592 637 672 700 485 506 580 618 477 499 568 602 502 528 541 564 1293 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 11 WEEK 12 WEEK 13 WEEK 14 WEEK 15 WEEK 16 WEEK 17 WEEK 21 GROUP: MALE 6 - 20 PPM C92854 477 493 501 510 516 527 530 553 C92855 450 456 464 471 480 493 487 499 C92856 441 448 454 461 474 484 487 503 C92857 512 517 520 527 537 544 551 572 C92858 503 518 532 553 563 575 576 608 C92859 479 488 498 510 517 526 525 547 C92860 433 442 442 454 465 465 475 491 GROUP: FEMALE 1 - 0 PPM C92862 241 241 243 246 261 260 257 253 C92863 277 281 284 290 287 290 287 303 C92865 276 280 292 284 286 293 289 322 C92866 279 289 296 296 296 304 304 317 C92867 300 329 324 323 318 324 311 329 C92868 283 290 302 311 315 314 308 316 C92869 235 239 244 248 256 259 259 267 C92870 259 265 272 272 276 290 287 291 C92871 287 295 295 299 292 291 296 307 C92872 224 233 234 235 240 239 244 274 C92873 226 236 243 246 246 253 256 261 C92874 273 281 287 290 293 294 298 306 C92875 251 264 261 263 272 283 282 286 C92876 258 266 266 272 271 279 278 290 C92877 259 264 267 277 272 279 289 295 C92878 256 261 269 262 272 272 279 283 C92879 310 327 320 325 331 331 331 332 C92880 267 274 273 283 C92881 286 295 301 296 306 314 313 321 C92882 245 244 243 251 250 249 258 264 C92883 327 337 344 338 346 352 350 357 Covance 6329-183 3M T-6295 PAGE: 37 WEEK 25 WEEK 29 583 626 524 559 525 543 601 629 631 663 573 602 522 549 258 268 315 333 337 344 320 345 346 363 335 343 273 280 307 316 324 351 291 309 269 279 321 324 292 299 298 301 326 328 285 293 357 380 333 360 254 272 367 383 1294 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 11 WEEK 12 WEEK 13 WEEK 14 WEEK 15 WEEK 16 WEEK 17 WEEK 21 GROUP: FEMALE 1 - 0 PPM C92884 265 264 281 275 267 273 276 286 C92885 296 304 307 313 317 324 316 330 C92886 255 260 260 266 269 265 275 283 C92887 277 287 289 296 C92888 315 332 343 357 356 370 370 369 C92889 254 261 260 260 264 266 264 278 C92890 323 330 329 338 342 343 343 350 C92891 256 262 259 273 281 279 275 285 C92892 348 361 366 370 369 371 375 382 C92893 250 248 250 257 260 260 259 259 C92894 255 264 272 277 281 281 279 295 C92895 279 288 289 297 295 296 295 306 C92896 273 280 285 298 303 301 308 328 C92897 243 247 250 260 260 263 261 274 C92898 290 296 300 306 C92899 346 362 374 366 366 375 386 399 C92900 310 322 299 331 329 330 329 345 C92901 263 270 275 280 279 288 289 296 C92902 368 374 383 398 417 420 408 418 C92903 351 353 378 378 C92904 255 265 262 273 265 273 261 275 C92905 256 267 267 280 C92906 252 254 257 262 264 265 267 275 C92907 258 277 278 284 292 289 299 296 C92908 288 292 303 315 313 314 319 321 C92909 269 281 287 289 291 297 297 299 C92910 305 316 319 325 333 339 324 306 C92911 241 253 260 255 263 269 266 282 C92912 294 308 317 318 322 335 351 353 C92913 281 288 292 292 294 296 299 298 C92915 271 287 297 297 302 310 304 307 Covance 6329-183 3M T-6295 PAGE: 38 WEEK 25 WEEK 29 287 307 350 359 288 312 399 405 284 296 367 377 298 306 409 426 265 281 304 315 322 324 352 370 284 290 413 414 363 369 306 318 447 470 273 278 289 296 316 322 356 369 313 321 330 347 281 293 366 356 310 314 323 338 1295 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 11 WEEK 12 WEEK 13 WEEK 14 WEEK 15 WEEK 16 WEEK 17 WEEK 21 GROUP: FEMALE 1 - 0 PPM C92916 336 357 369 388 403 414 426 439 C92917 346 359 354 367 372 381 375 390 C92918 274 292 295 295 305 313 313 311 C92920 252 264 267 277 280 285 284 290 C92921 318 338 353 355 373 380 369 389 C92922 282 291 300 298 304 307 299 314 C92923 246 243 253 260 268 270 271 285 C92924 301 312 316 315 319 324 320 320 C92925 315 325 326 330 332 333 331 339 C92927 282 286 281 287 288 292 291 295 C92928 286 290 302 304 309 311 323 331 C92929 268 269 281 278 281 288 288 289 C92930 361 388 406 421 435 448 455 473 GROUP: FEMALE 2 - 0.5 PPM C92931 333 336 334 348 348 370 368 395 C92932 272 264 270 280 282 290 294 305 C92933 318 337 337 353 355 355 367 390 C92934 254 263 266 271 275 277 281 286 C92935 304 307 311 321 315 320 327 341 C92936 266 263 270 273 275 274 285 288 C92937 279 279 285 291 293 296 304 318 C92938 298 310 309 307 311 323 322 341 C92939 245 239 246 244 247 254 257 272 C92940 259 256 265 270 268 272 278 286 C92942 314 314 317 325 334 335 334 338 C92943 320 315 319 324 330 343 344 348 C92944 365 380 369 380 C92946 259 249 260 267 259 264 268 274 C92947 300 302 305 311 300 307 306 324 Covance 6329-183 3M T-6295 PAGE: 39 WEEK 25 WEEK 29 457 472 409 425 324 336 309 326 420 445 332 345 299 303 333 336 358 373 315 324 352 344 312 327 510 532 417 461 306 305 415 452 300 313 359 374 286 307 330 342 354 358 274 295 295 302 347 354 345 361 292 301 326 342 1296 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 11 WEEK 12 WEEK 13 WEEK 14 WEEK 15 WEEK 16 WEEK 17 WEEK 21 GROUP: FEMALE 2 - 0.5 PPM C92948 322 327 329 338 327 337 340 360 C92949 388 383 395 415 421 438 437 449 C92951 258 250 243 256 264 271 265 276 C92952 269 278 276 283 291 294 298 315 C92953 262 264 261 270 272 273 273 286 C92954 281 277 285 288 292 293 294 301 C92955 297 308 309 319 312 324 329 343 C92956 317 324 326 332 324 337 341 357 C92957 267 272 272 274 282 288 284 297 C92958 245 241 248 255 251 254 262 272 C92959 313 313 319 332 333 336 341 337 C92960 304 301 314 316 323 326 325 331 C92961 366 376 386 399 408 418 419 432 C92962 261 263 269 274 C92963 249 244 250 262 263 265 267 280 C92964 271 275 279 283 286 300 292 308 C92965 275 270 280 284 285 290 291 297 C92966 249 250 250 257 259 259 261 269 C92967 299 297 303 310 C92968 317 313 318 328 337 343 340 353 C92970 334 325 336 347 354 355 359 361 C92971 246 249 249 248 260 251 257 259 C92972 287 279 287 297 297 304 312 332 C92973 259 263 259 271 282 286 287 288 C92974 253 261 265 267 269 277 283 290 C92975 271 277 280 288 287 291 295 305 C92976 326 326 331 333 341 347 341 350 C92977 295 295 301 313 317 322 323 330 C92978 349 347 363 366 C92979 277 281 281 289 299 304 302 300 C92980 290 303 311 312 328 336 329 343 Covance 6329-183 3M T-6295 PAGE: 40 WEEK 25 WEEK 29 373 387 461 485 274 273 326 336 288 294 305 308 378 397 374 384 307 323 283 288 358 363 361 375 461 473 278 284 329 346 309 304 274 276 370 390 381 387 263 268 335 350 313 329 294 296 313 331 371 393 348 349 317 330 379 379 1297 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 11 WEEK 12 WEEK 13 WEEK 14 WEEK 15 WEEK 16 WEEK 17 WEEK 21 GROUP: FEMALE 2 - 0.5 PPM C92981 300 292 307 303 316 323 313 331 C92983 313 301 310 325 326 329 324 333 C92984 317 320 325 333 330 337 337 349 C92985 316 313 322 338 330 343 342 356 C92986 267 269 272 272 267 279 279 288 C92987 287 288 286 287 C92988 281 283 280 283 298 309 303 318 C92989 273 278 279 284 290 290 288 311 C92990 282 285 287 283 292 299 296 296 GROUP: FEMALE 3 - 2 PPM C92991 250 250 259 261 265 267 273 278 C92992 265 272 272 273 275 286 282 301 C92993 289 292 291 302 C92994 272 277 278 285 273 287 285 302 C92997 253 255 255 269 264 267 273 289 C92998 290 295 304 309 309 307 318 324 C92999 302 295 299 308 299 314 315 335 C93000 285 286 290 293 C93001 256 261 268 271 274 280 279 298 C93002 267 259 263 280 277 276 288 302 C93003 305 300 315 324 327 333 337 329 C93004 273 274 283 287 288 298 294 310 C93005 257 255 265 275 282 291 289 313 C93007 267 264 273 277 272 275 280 291 C93009 265 275 288 289 294 290 300 301 C93010 277 280 289 295 292 301 308 314 C93011 294 299 299 309 311 311 323 321 C93012 316 321 325 328 330 337 347 365 C93013 240 246 250 256 261 263 269 273 Covance 6329-183 3M T-6295 PAGE: 41 WEEK 25 WEEK 29 361 349 338 354 364 374 358 375 293 309 331 353 326 339 303 325 284 305 318 331 301 304 289 296 339 367 342 356 316 347 307 317 343 361 321 326 316 341 303 320 333 320 318 338 337 341 376 397 281 285 1298 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 11 WEEK 12 WEEK 13 WEEK 14 WEEK 15 WEEK 16 WEEK 17 WEEK 21 GROUP: FEMALE 3 - 2 PPM C93014 317 308 325 325 328 336 331 359 C93015 301 295 306 307 311 316 318 324 C93016 228 213 231 239 235 242 246 249 C93017 269 272 277 292 304 308 308 314 C93018 286 290 296 299 C93019 301 305 317 321 313 324 335 373 C93020 282 284 290 294 309 309 308 325 C93021 287 299 295 300 304 308 317 336 C93022 308 306 307 315 315 319 321 333 C93023 246 250 256 257 C93024 274 279 289 294 287 297 302 305 C93025 324 323 339 359 360 379 354 389 C93026 279 279 283 291 285 287 289 294 C93027 276 273 279 285 296 298 297 299 C93028 272 276 273 276 282 295 291 299 C93029 249 257 255 264 267 268 273 278 C93030 269 265 283 293 288 291 301 310 C93032 271 264 279 283 281 289 297 315 C93033 277 279 279 294 303 309 319 332 C93034 290 291 289 301 303 303 305 303 C93035 292 292 297 304 C93036 280 278 284 297 305 307 305 330 C93037 274 279 288 299 301 316 333 316 C93038 239 241 247 257 257 256 268 271 C93039 291 303 304 312 316 319 316 325 C93040 229 229 239 246 250 248 255 260 C93041 283 288 285 306 305 302 312 338 C93042 297 293 303 316 324 318 316 343 C93043 283 276 292 303 314 317 324 334 C93044 255 256 265 267 264 265 281 294 C93046 318 304 315 317 324 325 333 344 Covance 6329-183 3M T-6295 PAGE: 42 WEEK 25 WEEK 29 354 381 335 340 268 268 328 349 388 405 353 336 363 377 342 367 314 320 353 411 299 313 311 315 311 321 285 298 322 342 326 339 352 374 317 325 359 388 300 317 279 293 330 350 266 276 351 379 359 393 347 364 304 314 335 350 1299 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WE1E1K WE12EK WEEK 13 WEEK 14 WEEK 15 WEEK 16 WEEK 17 WE21EK GROUP: FEMALE 3 - 2 PPM C93047 331 330 346 348 357 367 373 373 C93048 238 238 239 250 249 247 257 259 C93049 258 260 261 268 276 273 277 289 C93050 280 274 281 297 309 305 303 309 GROUP: FEMALE 4 - 5 PPM C93051 261 260 255 260 C93052 279 279 284 286 302 297 308 316 C93053 260 274 272 283 290 285 305 319 C93054 260 258 263 264 271 267 284 287 C93055 288 286 293 303 306 307 307 318 C93056 284 276 279 291 294 298 299 302 C93057 254 254 259 270 275 279 283 274 C93059 280 278 283 293 292 297 299 300 C93060 275 285 294 299 302 307 310 304 C93061 281 281 279 290 300 300 306 322 C93062 265 262 267 275 278 287 280 291 C93063 272 274 272 288 285 298 299 302 221C93064 252 255 257 266 C93065 223 228 232 238 235 240 249 C93066 267 266 275 282 278 289 278 298 C93068 274 275 278 285 289 294 295 305 C93069 328 329 336 346 352 356 358 369 C93071 244 239 248 249 C93072 272 272 278 283 288 293 289 308 C93073 278 273 279 288 295 303 302 310 C93074 298 298 304 313 314 316 322 345 C93076 279 290 296 296 297 299 303 319 C93077 296 299 305 315 314 319 321 339 C93078 270 266 268 278 275 283 279 296 Covance 6329-183 3M T-6295 PAGE: 43 WEEK 25 WEEK 29 401 410 270 282 295 301 311 319 335 340 361 381 293 303 332 346 313 332 296 309 315 337 320 318 354 379 296 311 308 324 260 269 302 342 326 332 400 417 314 333 315 321 368 391 336 356 364 379 305 311 1300 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 11 WEEK 12 WEEK 13 WEEK 14 WEEK 15 WEEK 16 WEEK 17 WEEK 21 GROUP: FEMALE 4 - 5 PPM C93079 305 291 307 308 320 332 323 345 C93080 264 269 281 282 292 292 294 298 C93081 259 254 267 271 271 276 280 285 C93082 294 292 294 304 302 306 318 319 C93083 284 279 280 285 298 300 303 307 C93085 269 277 285 290 C93086 287 295 299 309 314 314 317 324 C93087 238 243 252 253 252 260 261 269 C93088 261 253 273 275 288 282 294 297 C93089 292 300 308 305 308 320 314 327 C93090 253 257 262 267 262 281 275 279 C93091 297 298 305 305 319 320 321 330 C93092 254 263 267 268 265 273 276 288 C93093 326 323 333 350 331 347 347 353 C93094 273 274 279 287 287 304 308 287 C93095 260 261 270 283 286 291 288 291 C93096 306 311 318 324 341 341 347 364 C93097 261 263 271 276 275 280 286 292 C93098 256 261 272 269 279 284 281 287 C93099 269 276 287 297 298 304 310 298 C93100 280 279 290 295 295 291 305 297 C93101 257 264 265 260 268 270 271 275 C93102 301 306 315 323 324 333 335 347 C93103 280 285 288 305 309 311 314 325 C93104 360 367 380 392 400 405 411 423 C93105 291 289 302 300 305 312 309 312 C93106 253 262 264 263 266 278 278 279 C93107 274 276 282 290 290 293 296 302 C93108 264 265 268 278 274 283 283 279 C93109 267 274 278 287 C93110 364 386 403 425 438 446 459 479 Covance 6329-183 3M T-6295 PAGE: 44 WEEK 25 WEEK 29 349 368 306 320 304 306 350 378 326 336 329 333 276 283 306 318 329 335 294 295 352 368 306 318 374 380 299 309 310 317 406 441 308 327 302 313 318 349 324 330 288 296 359 363 338 331 449 464 325 329 291 305 309 323 287 304 496 538 1301 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 11 WEEK 12 WEEK 13 WEEK 14 WEEK 15 WEEK 16 WEEK 17 WEEK 21 GROUP: FEMALE 5 - 20 PPM C93111 311 300 305 312 C93112 271 275 278 279 284 283 285 299 C93113 229 240 242 237 241 251 248 253 C93115 224 223 223 228 235 233 234 243 C93116 248 242 250 254 256 254 263 267 C93117 258 259 265 261 271 266 279 290 C93118 236 232 234 246 250 254 261 275 C93119 220 216 220 224 223 233 232 245 C93120 244 235 244 244 240 251 249 256 C93121 218 219 222 218 223 226 227 235 C93122 293 295 303 299 305 305 311 311 C93124 246 258 271 288 270 264 264 276 C93125 216 220 221 218 227 232 232 237 C93126 226 229 233 231 239 243 242 256 C93127 255 251 253 258 C93128 250 249 258 266 268 266 272 276 C93129 248 265 256 266 271 259 271 306 C93130 268 274 281 281 290 289 292 298 C93131 278 277 284 291 287 293 302 309 C93132 258 268 264 268 269 277 274 298 C93133 238 242 245 241 250 249 253 262 C93134 281 286 291 285 287 296 297 311 C93135 273 269 273 275 275 284 288 297 C93136 248 243 259 265 262 269 280 275 C93137 240 236 245 242 249 252 255 264 C93138 269 261 271 271 275 280 290 292 C93139 224 226 232 238 236 240 245 262 C93140 271 275 276 282 285 298 307 324 C93141 290 286 286 292 294 298 300 316 C93143 291 277 287 293 C93145 230 231 226 235 235 232 240 248 Covance 6329-183 3M T-6295 PAGE: 45 WEEK 25 WEEK 29 309 325 262 267 253 262 273 280 296 305 293 276 253 251 267 278 246 250 329 344 272 286 244 254 266 285 289 295 282 287 317 319 320 327 315 332 275 267 318 328 275 289 292 301 271 284 304 308 264 272 343 367 324 341 255 263 1302 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 11 WEEK 12 WEEK 13 WEEK 14 WEEK 15 WEEK 16 WEEK 17 WEEK 21 GROUP: FEMALE 5 - 20 PPM C93146 236 231 226 240 238 238 244 245 C93147 258 250 251 260 259 261 266 268 C93148 294 290 289 301 303 306 307 324 C93149 256 253 258 258 266 269 267 280 C93150 278 276 295 295 283 290 289 295 C93151 262 260 260 266 268 270 268 275 C93152 270 271 273 277 278 277 278 282 C93153 248 245 252 252 255 252 255 264 C93154 269 266 262 266 266 274 274 278 C93155 249 249 247 243 C93156 245 243 244 250 248 250 255 259 C93157 256 259 264 265 263 266 270 273 C93158 269 271 272 278 275 274 281 292 C93160 308 299 306 318 324 330 330 334 C93161 268 273 275 287 278 290 290 294 C93162 287 278 280 275 280 277 291 298 C93163 244 247 248 252 253 261 258 260 C93164 245 244 239 244 247 248 255 256 C93165 255 250 255 260 261 262 261 277 C93166 310 302 313 316 315 318 326 325 C93167 284 272 288 296 288 305 302 318 C93168 248 243 239 247 253 245 256 266 C93169 247 248 249 257 C93170 273 269 267 276 277 283 286 285 C93171 266 267 273 276 276 279 282 291 C93172 265 267 268 275 279 280 280 282 C93173 258 257 262 272 264 271 274 280 C93174 249 256 253 259 270 267 268 279 C93175 253 246 250 255 258 255 260 259 C93176 249 250 254 259 267 266 279 288 C93177 240 240 242 249 247 245 247 259 Covance 6329-183 3M T-6295 PAGE: 46 WEEK 25 WEEK 29 248 244 274 283 350 356 288 286 302 308 281 289 290 297 271 270 282 286 266 277 279 289 298 314 346 366 306 326 312 318 274 288 262 271 281 292 358 347 349 359 271 279 305 317 292 318 295 317 291 290 289 297 275 283 307 323 266 269 1303 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 11 WEEK 12 WEEK 13 WEEK 14 WEEK 15 WEEK 16 WEEK 17 WEEK 21 GROUP: FEMALE 5 - 20 PPM C93178 251 253 250 255 252 258 257 267 C93179 244 241 241 250 248 251 250 259 C93180 298 293 297 299 299 305 311 315 GROUP: FEMALE 6 - 20 PPM C93181 252 255 255 259 267 268 265 272 C93182 245 247 245 257 261 268 257 268 C93183 287 290 295 297 303 309 312 313 C93184 233 238 235 241 244 239 244 242 C93185 260 265 265 273 280 282 278 287 C93186 224 226 228 221 232 228 236 239 C93187 256 269 267 270 280 282 292 318 C93188 267 272 281 290 290 292 295 302 C93189 237 242 242 243 249 246 242 249 C93190 263 265 274 274 275 280 280 289 C93191 319 309 310 316 317 311 319 323 C93192 236 234 241 247 246 248 247 262 C93193 249 256 260 262 274 266 278 294 C93194 293 300 306 308 308 316 315 320 C93195 272 278 280 285 288 290 291 291 C93196 226 231 229 235 238 240 244 250 C93197 222 225 232 237 234 233 238 236 C93198 251 257 266 271 273 278 277 280 C93199 250 255 260 262 263 266 267 271 C93200 238 245 245 255 256 257 255 261 C93201 238 236 234 243 247 249 249 257 C93202 247 244 248 254 253 251 242 258 C93203 264 275 270 276 278 282 292 313 C93204 249 255 253 255 258 267 268 273 C93205 283 291 295 295 296 302 300 305 Covance 6329-183 3M T-6295 PAGE: 47 WEEK 25 WEEK 29 271 283 264 265 327 337 284 291 269 277 328 356 253 260 299 286 251 242 345 358 318 331 259 259 300 315 342 343 267 282 314 341 327 334 305 315 260 269 239 245 292 301 275 286 270 280 261 271 263 268 324 339 282 293 309 308 1304 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 11 WEEK 12 WEEK 13 WEEK 14 WEEK 15 WEEK 16 WEEK 17 WEEK 21 GROUP: FEMALE 6 - 20 PPM C93206 230 232 239 241 239 250 245 263 C93207 234 233 243 247 247 249 256 261 C93208 240 244 250 256 256 260 265 264 C93209 247 247 249 254 254 257 260 262 C93210 268 266 271 285 281 287 293 295 C93211 234 232 238 238 243 248 248 241 C93212 272 285 282 280 284 286 292 300 C93213 253 265 259 269 268 272 272 278 C93214 240 246 248 253 254 261 267 269 C93215 263 268 275 276 275 282 282 289 C93216 249 252 261 263 260 264 270 273 C93217 291 297 304 306 313 316 312 323 C93218 233 237 239 246 246 252 247 259 C93219 301 302 293 309 310 309 313 312 C93220 278 284 299 303 308 314 315 334 Covance 6329-183 3M T-6295 PAGE: 48 WEEK 25 WEEK 29 272 289 269 277 274 277 275 285 310 319 244 251 315 326 288 303 285 295 301 315 295 313 340 344 268 278 327 341 344 359 1305 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 33 WEEK 37 WEEK 41 WEEK 45 WEEK 49 WEEK 53 WEEK 57 WEEK 61 GROUP: MALE 1 - 0 PPM C92501 796 822 812 835 855 890 C92502 578 593 596 603 617 631 646 647 C92503 655 674 695 713 729 673 700 759 C92504 591 607 621 633 673 690 C92505 551 580 609 617 632 649 666 686 C92507 618 621 631 647 659 675 C92508 589 607 600 613 617 651 669 683 C92510 660 685 688 716 711 713 731 726 C92512 635 662 676 691 693 684 C92513 642 662 655 683 684 694 713 718 C92514 821 842 846 854 836 866 868 875 C92515 609 641 649 577 641 674 696 715 C92516 606 622 625 644 C92517 821 854 870 897 922 938 C92518 734 750 759 783 790 816 835 875 C92519 592 610 618 631 638 650 665 691 C92520 637 668 664 693 706 711 710 720 C92522 616 644 645 678 693 720 750 766 C92523 693 722 718 757 777 805 C92525 695 727 741 764 776 795 812 679 C92527 674 697 706 731 757 767 779 772 C92530 675 693 700 715 734 739 731 758 C92531 648 671 662 670 665 671 669 659 C92533 727 765 777 806 820 846 865 873 C92534 627 647 640 642 666 665 C92535 671 698 690 617 692 729 750 766 C92536 681 701 703 738 746 774 792 805 C92537 857 891 905 937 961 966 984 1015 C92538 774 821 866 911 968 1008 1071 1109 C92539 795 822 816 835 846 884 901 924 C92540 719 755 779 807 827 855 C92541 753 765 792 812 830 849 864 893 Covance 6329-183 3M T-6295 PAGE: 49 WEEK 65 WEEK 69 653 766 697 680 735 710 912 729 877 688 708 787 550 803 765 665 890 794 837 1024 1150 941 897 668 780 711 680 746 727 949 737 884 700 684 804 506 819 775 901 811 815 1064 1192 950 920 1306 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 33 WEEK 37 WEEK 41 WEEK 45 WEEK 49 WEEK 53 WEEK 57 WEEK 61 GROUP: MALE 1 - 0 PPM C92542 615 632 645 664 687 705 704 730 C92543 620 634 632 651 669 685 697 703 C92544 632 618 629 615 629 642 643 656 C92545 678 715 736 746 795 824 846 869 C92547 739 754 758 770 794 794 805 814 C92548 664 688 708 735 757 769 775 791 C92549 544 555 567 588 614 622 631 652 C92550 757 780 803 832 870 889 904 914 C92551 576 593 610 619 638 645 664 673 C92552 712 731 738 769 799 826 843 850 C92553 384 401 372 377 390 371 366 353 C92554 577 591 597 610 637 648 645 658 C92555 736 754 791 806 808 828 784 C92556 693 717 710 739 770 767 672 499 C92557 651 680 693 715 725 732 755 775 C92558 722 752 755 770 784 793 800 811 C92559 613 643 661 668 692 707 715 738 C92560 691 713 722 746 771 792 807 831 C92561 675 701 715 729 753 756 774 747 C92562 641 656 665 681 711 703 C92563 683 684 689 682 701 704 720 732 C92564 786 823 821 850 870 877 883 894 C92565 707 721 741 770 800 816 824 850 C92567 688 692 702 729 753 772 784 818 C92568 670 685 687 709 725 745 754 759 C92569 640 655 644 659 667 668 679 681 C92570 661 665 667 680 696 686 Covance 6329-183 3M T-6295 PAGE: 50 WEEK 65 WEEK 69 729 749 711 724 629 662 888 911 826 837 790 783 619 667 937 947 683 716 871 895 662 684 800 805 818 819 744 758 833 839 717 717 904 826 864 868 829 849 779 785 686 683 1307 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 33 WEEK 37 WEEK 41 WEEK 45 WEEK 49 WEEK 53 WEEK 57 WEEK 61 GROUP: MALE 2 - 0.5 PPM C92571 689 695 706 722 767 786 800 833 C92572 696 716 734 749 774 772 801 827 C92573 814 837 823 858 892 910 929 946 C92576 721 748 771 797 819 828 868 887 C92577 811 838 851 882 907 917 936 963 C92578 720 748 779 804 837 856 888 911 C92579 757 780 788 804 813 822 842 851 C92580 687 706 716 740 767 780 798 829 C92581 719 739 758 787 808 829 849 873 C92582 574 586 590 587 617 629 627 642 C92583 587 599 622 641 665 700 718 724 C92584 539 551 550 558 582 594 576 C92585 697 575 C92586 749 795 833 865 892 921 944 911 C92587 777 797 803 813 836 851 836 844 C92588 617 638 635 653 667 683 674 672 C92589 707 729 729 757 790 810 824 826 C92590 712 731 748 764 790 801 812 816 C92591 652 677 688 703 726 745 753 761 C92592 699 723 736 759 789 807 813 837 C92594 632 654 665 670 694 712 720 721 C92595 607 626 622 631 657 684 691 701 C92596 678 699 706 719 733 750 756 752 C92597 591 605 604 614 629 647 657 664 C92598 645 660 665 679 701 707 713 728 C92599 721 749 758 787 815 816 C92601 639 661 674 700 726 736 756 758 C92602 533 563 563 565 600 602 599 599 C92604 739 768 795 820 843 867 877 913 C92605 765 787 812 824 854 848 863 867 C92606 671 696 714 733 758 766 768 769 Covance 6329-183 3M T-6295 PAGE: 51 WEEK 65 WEEK 69 852 869 832 854 956 850 901 920 966 965 938 965 838 842 849 872 907 934 658 656 739 744 912 748 850 856 685 685 827 826 833 845 782 801 859 875 736 756 703 695 729 575 667 686 746 745 768 789 600 604 942 950 887 886 764 757 1308 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 33 WEEK 37 WEEK 41 WEEK 45 WEEK 49 WEEK 53 WEEK 57 WEEK 61 GROUP: MALE 2 - 0 .5 PPM C92607 700 731 754 778 794 799 815 842 C92608 745 741 784 786 813 832 870 868 C92609 652 663 692 714 743 752 764 768 C92611 605 623 629 636 647 652 665 665 C92612 696 721 741 763 787 805 823 844 C92614 679 693 699 709 718 730 722 726 C92615 732 766 781 811 838 862 873 884 C92617 804 829 852 891 894 889 871 824 C92619 578 576 583 599 624 620 636 650 C92620 554 567 575 595 603 601 614 611 C92622 694 721 739 772 796 805 822 839 C92623 598 608 612 631 636 645 659 666 C92624 688 726 755 768 804 827 829 783 C92625 777 804 826 843 874 886 887 901 C92626 655 632 653 661 697 703 710 721 C92628 694 705 698 738 768 698 758 628 C92629 776 805 834 871 906 924 918 922 C92630 694 719 731 742 755 757 764 750 GROUP: MALE 3 - 2 PPM C92631 600 625 632 653 670 674 679 698 C92632 801 837 857 869 888 901 914 901 C92633 670 697 715 740 766 785 787 797 C92634 617 646 655 688 715 733 737 770 C92635 681 696 714 739 755 764 757 780 C92636 666 689 705 734 743 771 792 823 C92637 551 560 574 595 614 625 629 661 C92638 877 917 931 955 988 998 1003 1020 C92639 664 696 706 721 743 755 770 783 C92641 667 689 726 735 723 726 749 717 Covance 6329-183 3M T-6295 PAGE: 52 WEEK 65 WEEK 69 849 836 781 679 864 732 913 810 650 616 848 668 900 696 557 919 747 705 861 817 787 766 827 676 1007 798 732 861 790 792 685 870 751 918 818 660 629 863 670 891 736 513 869 733 718 848 835 791 775 841 691 1002 792 729 1309 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 33 WEEK 37 WEEK 41 WEEK 45 WEEK 49 WEEK 53 WEEK 57 WEEK 61 GROUP: MALE 3 - 2 PPM C92642 685 697 705 721 737 751 751 764 C92643 574 581 587 599 616 624 623 636 C92644 555 577 578 589 608 625 632 660 C92647 626 644 654 673 700 708 714 739 C92648 565 591 598 619 624 640 648 669 C92649 547 559 568 585 598 610 617 635 C92651 691 719 730 744 780 799 818 844 C92653 739 767 795 820 841 864 842 879 C92654 637 666 667 665 680 682 689 699 C92655 721 736 758 775 795 804 812 821 C92656 706 738 742 756 777 789 799 815 C92657 664 694 700 705 721 733 742 753 C92658 708 727 744 762 781 804 815 837 C92659 718 735 751 772 784 792 800 823 C92660 552 567 570 586 596 603 601 606 C92661 648 638 642 654 686 703 715 714 C92663 749 779 795 825 843 879 886 924 C92664 672 704 707 714 740 756 760 758 C92665 756 775 794 811 827 844 856 891 C92666 679 705 717 738 771 780 799 805 C92667 733 755 786 802 817 821 819 856 C92669 658 678 705 717 739 755 759 783 C92670 553 570 584 593 598 594 582 569 C92671 622 664 674 675 697 685 705 727 C92672 660 685 713 728 755 782 800 825 C92673 656 676 687 713 728 737 761 784 C92674 704 723 747 771 783 809 822 850 C92675 596 615 630 655 667 683 701 721 C92677 612 635 648 666 681 685 705 728 C92679 709 748 765 811 849 845 C92680 704 720 731 736 760 778 795 802 Covance 6329-183 3M T-6295 PAGE: 53 WEEK 65 WEEK 69 763 772 638 656 671 678 748 767 685 689 644 664 841 805 889 909 710 726 832 850 836 843 759 760 846 860 817 821 603 615 721 735 924 935 760 769 915 908 823 745 864 894 804 812 530 481 734 759 852 863 814 832 864 876 732 750 734 753 799 646 1310 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 33 WEEK 37 WEEK 41 WEEK 45 WEEK 49 WEEK 53 WEEK 57 WEEK 61 GROUP: MALE 3 - 2 PPM C92681 650 672 684 704 716 739 748 765 C92682 652 676 697 720 738 753 778 798 C92683 697 726 757 782 800 820 818 809 C92685 711 737 750 773 800 805 825 837 C92686 654 684 709 734 764 787 799 832 C92687 745 704 758 787 779 784 780 766 C92688 586 590 582 610 623 634 638 648 C92689 609 621 647 667 689 693 C92690 795 817 835 863 895 916 934 961 GROUP: MALE 4 - 5 PPM C92691 641 671 675 700 707 720 721 748 C92692 642 660 680 673 632 C92693 654 674 679 704 721 733 731 754 C92694 675 700 720 739 758 778 778 801 C92695 611 629 648 664 675 686 690 692 C92696 598 616 618 651 678 684 685 703 C92697 626 645 657 676 703 709 721 743 C92698 718 735 760 794 829 856 890 921 C92699 555 576 583 593 586 593 597 606 C92700 471 493 507 533 546 553 563 570 C92701 659 670 688 709 730 560 C92702 590 607 619 633 643 655 671 686 C92703 684 711 739 760 785 811 804 686 C92704 685 715 723 743 755 773 779 808 C92705 650 683 702 726 748 766 771 772 C92706 712 742 785 825 861 878 900 933 C92707 582 594 608 630 638 649 658 675 C92708 574 596 611 637 655 668 663 682 C92709 593 596 604 628 655 669 665 681 Covance 6329-183 3M T-6295 PAGE: 54 WEEK 65 WEEK 69 776 771 806 820 844 886 854 867 855 881 691 545 658 668 986 1009 758 772 770 777 807 838 708 720 728 728 643 944 977 631 634 579 590 699 701 823 843 793 807 978 1001 692 703 698 709 685 675 1311 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 33 WEEK 37 WEEK 41 WEEK 45 WEEK 49 WEEK 53 WEEK 57 WEEK 61 GROUP: MALE 4 - 5 PPM C92710 616 637 652 670 692 702 703 712 C92711 645 667 679 701 721 745 746 760 C92712 739 761 779 816 842 863 878 877 C92716 733 734 673 647 629 618 566 531 C92717 684 710 720 738 762 779 782 802 C92720 690 721 739 770 791 816 824 832 C92721 570 587 603 590 598 621 624 651 C92723 701 717 729 746 769 767 791 820 C92724 740 767 784 822 851 871 878 907 C92725 576 596 609 613 610 C92726 596 611 623 647 666 679 687 695 C92727 667 700 714 747 780 796 790 813 C92729 785 812 850 879 903 917 937 960 C92730 649 677 674 690 714 740 747 769 C92732 647 689 707 738 755 773 774 796 C92733 567 582 612 628 644 656 666 682 C92735 652 676 685 704 717 732 741 759 C92736 663 700 719 737 769 788 793 821 C92737 653 686 698 715 728 753 767 788 C92738 599 624 633 649 659 665 682 695 C92739 562 568 585 598 613 625 630 657 C92740 651 661 668 681 697 714 731 746 C92741 636 650 663 682 697 718 711 725 C92742 789 813 834 844 862 886 889 906 C92743 780 829 859 883 905 931 965 984 C92745 820 867 904 941 967 1010 1029 1065 C92746 736 759 770 778 795 819 827 850 C92747 632 666 672 681 696 722 726 757 C92748 682 710 729 732 750 767 764 787 C92749 593 608 636 658 672 686 695 703 C92750 655 681 696 708 725 729 755 771 Covance 6329-183 3M T-6295 PAGE: 55 WEEK 65 WEEK 69 711 787 894 566 821 858 657 830 937 717 845 888 778 812 690 772 835 798 714 670 750 738 904 1017 1104 853 779 784 723 794 720 800 905 545 828 884 673 837 954 723 845 790 797 820 695 770 854 805 733 682 769 745 922 1026 1123 886 792 809 729 812 1312 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 33 WEEK 37 WEEK 41 WEEK 45 WEEK 49 WEEK 53 WEEK 57 WEEK 61 GROUP: C92751 C92753 C92754 C92755 C92756 C92757 C92758 C92760 C92761 C92762 C92763 C92764 C92766 C92767 C92768 C92769 C92770 C92771 C92772 C92773 C92774 C92775 C92776 C92778 C92780 C92781 C92782 C92783 C92784 C92785 C92786 MALE 5 - 20 PPM 710 722 581 616 518 542 620 651 701 739 557 566 589 612 604 628 532 552 597 629 830 886 642 673 587 625 857 921 669 706 665 670 687 724 673 716 620 648 496 515 652 664 697 728 642 659 648 680 525 550 587 607 613 645 701 745 615 644 582 611 630 663 764 791 818 843 643 644 617 640 663 682 553 558 573 588 671 689 699 732 761 774 800 815 834 836 587 597 603 617 634 656 635 641 630 576 510 423 648 663 685 707 730 754 572 578 594 604 617 603 649 678 712 727 760 789 919 962 992 1017 1044 1082 690 703 731 741 758 765 637 650 654 638 632 650 974 1026 1088 1114 1124 1196 722 731 766 777 789 791 696 706 724 743 764 780 758 783 826 846 877 904 734 665 766566 770 684 799 824 859 539 546 559 560 565 564 683 695 708 730 742 745 765 751 800 813 831 843 678 686 700 708 729 736 683 701 726 724 561 583 596 619 631 640 626 639 660 671 682 705 681 708 735 755 766 802 769 796 813 846 878 910 677 698 729 749 778 784 630 646 663 678 702 719 684 696 716 726 752 769 Covance 6329-183 3M T-6295 PAGE: 56 WEEK 65 WEEK 69 698 866683 765 619 799 1080 788 653 1225 808 799 923 876 549 744 863 750 640 718 831 926 806 731 785 719 865 683 713 641 816 1100 746 659 1246 817 815 916 905 557 757 878 755 654 708 862 938 814 752 790 1313 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 33 WEEK 37 WEEK 41 WEEK 45 WEEK 49 WEEK 53 WEEK 57 WEEK 61 GROUP: MALE 5 - 20 PPM C92787 600 607 625 589 C92788 625 648 661 683 697 715 720 745 C92790 597 611 625 638 655 667 C92791 574 609 639 661 693 717 733 754 C92792 625 643 646 665 678 697 704 722 C92794 573 598 602 625 632 630 541 C92795 621 640 664 679 702 710 C92796 521 544 556 570 583 573 C92797 610 635 662 680 691 694 711 725 C92800 572 594 586 586 610 625 635 662 C92801 767 818 846 855 880 910 C92802 625 646 666 696 733 752 786 824 C92803 599 622 618 623 644 657 667 676 C92804 602 632 661 678 713 732 740 769 C92805 681 694 716 720 739 747 783 802 C92806 631 640 669 664 670 680 694 684 C92807 629 670 679 690 725 737 760 789 C92808 534 554 555 553 574 583 591 602 C92809 602 623 641 648 661 672 684 697 C92810 622 650 671 686 716 737 746 780 C92811 677 C92813 612 637 651 679 698 722 741 747 C92814 456 471 470 478 495 512 517 535 C92815 522 535 538 545 557 573 590 604 C92816 569 584 593 605 625 630 649 665 C92817 809 853 890 933 982 1008 C92818 591 621 626 632 659 672 685 696 C92819 537 562 578 583 617 633 654 669 C92820 504 516 520 497 530 537 Covance 6329-183 3M T-6295 PAGE: 57 WEEK 65 WEEK 69 771 776 768 786 747 755 737 754 664 665 829 865 679 697 792 813 816 692 711 719 801 818 609 617 710 726 796 809 769 778 541 540 609 616 712 714 687 700 1314 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 33 WEEK 37 WEEK 41 WEEK 45 WEEK 49 WEEK 53 WEEK 57 WEEK 61 GROUP: C92821 C92822 C92823 C92824 C92825 C92826 C92827 C92828 C92829 C92830 C92831 C92832 C92833 C92834 C92835 C92836 C92837 C92838 C92839 C92840 C92841 C92842 C92843 C92844 C92845 C92846 C92847 C92848 C92849 C92850 C92851 MALE 6 - 20 PPM 546 570 615 630 595 616 639 654 787 823 600 630 601 625 634 655 704 734 574 576 639 644 555 571 715 758 620 631 591 608 635 656 673 699 613 628 866 917 557 586 530 569 665 689 676 712 475 491 571 585 670 709 735 784 529 548 646 672 504 537 613 654 577 589 600 605 631 640 638 648 679 703 744 749 628 640 646 664 663 681 651 669 693 699 718 747 863 911 961 998 1036 1081 641 655 683 703 636 648 672 683 748 744 669 671 696 714 745 764 740 770 789 800 817 849 572 593 608 591 587 605 637 636 660 682 699 701 571 585 602 610 630 649 782 814 856 882 918 957 661 668 690 702 726 742 621 631 644 647 660 672 681 706 727 742 771 660 716 730 751 772 794 722 646 660 685 697 717 745 966 1003 1043 1059 1107 1103 598 610 631 648 667 670 582 594 613 624 652 680 696 718 745 765 756 786 728 756 790 808 849 879 486 481 497 509 514 524 590 606 620 619 634 655 716 749 774 789 762 781 806 820 855 874 913 938 551 549 583 588 585 576 692 723 747 764 794 808 550 560 581 589 589 620 670 687 714 731 767 786 Covance 6329-183 3M T-6295 PAGE: 58 WEEK 65 WEEK 69 639 767 709 767 974 756 790 870 628 701 651 983 755 691 785 464 644 1118 696 716 804 908 523 653 790 941 542 727 635 799 638 753 732 785 1038 767 796 902 629 700 636 992 766 698 821 1140 705 809 937 535 661 777 950 525 537 663 812 1315 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 33 WEEK 37 WEEK 41 WEEK 45 WEEK 49 WEEK 53 WEEK 57 WEEK 61 GROUP: MALE 6 - 20 PPM C92852 557 582 593 613 641 650 680 685 C92853 597 615 632 648 674 676 717 752 C92854 647 679 693 716 743 759 783 820 C92855 573 594 618 619 631 664 685 646 C92856 565 572 581 594 618 625 653 661 C92857 646 677 702 714 744 785 809 736 C92858 684 696 701 717 748 765 794 811 C92859 613 630 638 661 680 690 703 683 C92860 562 570 572 586 585 608 622 646 GROUP: FEMALE 1 - 0 PPM C92862 269 278 283 304 315 310 C92863 369 369 396 389 400 434 452 474 C92865 361 381 397 408 421 428 444 461 C92866 356 340 358 377 408 413 421 438 C92867 380 384 405 414 433 440 467 485 C92868 360 376 389 407 420 423 442 444 C92869 290 293 300 312 345 328 357 366 C92870 338 346 364 380 389 410 422 440 C92871 367 371 384 397 416 435 452 474 C92872 336 348 353 377 392 401 422 435 C92873 289 290 313 328 339 352 362 378 C92874 355 377 401 426 437 453 467 481 C92875 316 322 350 369 385 392 412 414 C92876 326 330 347 370 377 384 C92877 357 382 393 417 433 438 454 481 C92878 308 311 328 349 363 374 401 401 C92879 399 424 441 462 475 483 485 512 C92881 361 374 388 414 424 431 447 459 C92882 288 300 311 341 353 367 375 398 Covance 6329-183 3M T-6295 PAGE: 59 WEEK 65 WEEK 69 691 773 798 847 880 557 481 661 678 805 862 837 834 617 544 663 670 482 498 451 456 481 501 449 456 374 386 453 482 506 531 434 459 381 403 488 506 398 422 492 493 419 441 511 537 467 467 413 418 1316 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 33 WEEK 37 WEEK 41 WEEK 45 WEEK 49 WEEK 53 WEEK 57 WEEK 61 GROUP: FEMALE 1 - 0 PPM C92883 388 382 398 412 418 424 442 471 C92884 313 327 344 356 367 385 390 410 C92885 375 399 418 418 457 462 476 494 C92886 323 347 373 398 415 426 440 441 C92888 436 480 499 538 549 568 599 623 C92889 319 348 347 357 385 398 C92890 377 385 395 417 436 463 473 493 C92891 318 331 337 349 358 358 370 380 C92892 442 479 504 538 577 580 591 641 C92893 290 295 304 312 315 323 332 339 C92894 323 326 342 352 358 363 C92895 321 332 351 367 374 374 401 424 C92896 373 384 404 421 437 440 468 476 C92897 294 299 312 333 344 348 366 382 C92899 430 450 462 469 483 478 C92900 368 396 423 443 466 488 520 533 C92901 333 353 359 375 386 392 421 423 C92902 514 531 571 607 628 648 680 704 C92904 290 278 307 334 344 321 347 386 C92906 317 327 338 348 359 362 385 383 C92907 335 372 348 353 359 358 372 389 C92908 377 408 445 465 485 507 535 551 C92909 327 355 354 369 387 384 397 418 C92910 349 359 377 403 412 420 C92911 305 312 313 331 345 351 350 355 C92912 386 420 428 473 508 522 560 593 C92913 323 326 327 325 347 347 358 371 C92915 363 392 414 441 453 470 502 514 C92916 503 527 548 570 579 579 607 640 C92917 454 474 501 522 542 560 C92918 344 364 380 399 423 426 457 485 Covance 6329-183 3M T-6295 PAGE: 60 WEEK 65 WEEK 69 471 470 405 426 492 513 442 402 645 669 500 515 382 393 652 668 336 350 416 436 486 505 388 390 554 577 432 444 726 763 401 417 397 404 407 414 560 573 422 431 357 368 611 640 381 390 526 542 654 686 492 476 1317 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 33 WEEK 37 WEEK 41 WEEK 45 WEEK 49 WEEK 53 WEEK 57 WEEK 61 GROUP: FEMALE 1 - 0 PPM C92920 330 340 348 371 375 383 404 413 C92921 486 514 542 554 477 350 306 C92922 370 398 426 462 477 487 C92923 319 340 361 381 393 403 C92924 347 365 372 396 417 422 449 472 C92925 395 417 432 464 487 492 524 543 C92927 334 338 354 366 372 371 387 394 C92928 356 387 425 456 477 491 525 546 C92929 360 347 364 391 347 304 294 C92930 559 593 617 646 666 668 GROUP: FEMALE 2 - 0.5 PPM C92931 499 519 564 596 607 640 667 715 C92932 330 316 267 C92933 484 508 519 519 531 545 554 581 C92934 310 336 363 370 382 413 432 446 C92935 415 427 463 479 496 518 550 579 C92936 320 336 352 362 368 377 387 389 C92937 356 374 393 408 420 431 370 388 C92938 364 397 408 431 463 493 522 552 C92939 300 321 339 351 360 374 389 378 C92940 306 310 326 347 354 372 398 399 C92942 360 370 389 427 438 C92943 404 427 458 472 489 503 533 540 C92946 335 352 380 401 408 421 423 426 C92947 340 347 368 383 385 411 421 425 C92948 408 431 451 473 485 510 552 562 C92949 472 C92951 282 290 323 327 317 325 356 372 C92952 344 363 386 398 411 415 429 438 Covance 6329-183 3M T-6295 PAGE: 61 WEEK 65 WEEK 69 421 442 478 485 572 596 391 401 569 594 755 758 591 591 438 439 603 619 398 414 283 584 615 379 410 429 437 550 585 447 480 436 440 570 586 387 401 357 1318 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 33 WEEK 37 WEEK 41 WEEK 45 WEEK 49 WEEK 53 WEEK 57 WEEK 61 GROUP: FEMALE 2 - 0.5 PPM C92953 301 305 337 328 343 344 360 368 C92954 321 340 356 373 389 402 415 436 C92955 422 443 475 485 492 507 544 569 C92956 408 418 443 466 489 502 529 537 C92957 337 360 355 389 395 406 434 438 C92958 304 320 340 354 366 376 385 401 C92959 371 389 392 431 437 440 465 418 C92960 395 414 423 459 473 481 501 509 C92961 504 532 557 580 591 613 645 667 C92963 315 327 335 345 354 376 364 392 C92964 362 371 387 398 412 418 418 442 C92965 327 328 343 364 378 393 419 436 C92966 281 287 293 302 304 305 315 330 C92968 400 445 464 431 408 402 405 411 C92970 408 427 442 470 488 516 530 554 C92971 266 276 277 286 294 297 302 313 C92972 362 372 391 400 417 419 445 445 C92973 348 383 400 440 466 487 508 532 C92974 300 326 344 352 356 365 390 395 C92975 333 352 374 385 389 402 413 420 C92976 411 431 450 481 497 525 546 540 C92977 360 382 416 428 449 455 481 488 C92979 334 353 377 392 407 409 436 454 C92980 373 412 425 458 453 466 432 426 C92981 381 372 394 426 431 437 460 474 C92983 366 373 386 406 414 419 445 450 C92984 384 389 410 433 447 453 474 477 C92985 421 400 434 465 480 477 499 540 C92986 310 318 326 341 347 348 362 368 C92988 359 374 404 422 447 445 463 478 C92989 353 361 377 398 409 390 397 419 Covance 6329-183 3M T-6295 PAGE: 62 WEEK 65 WEEK 69 375 379 456 479 588 607 555 572 460 461 408 432 473 455 533 548 677 710 390 401 454 479 458 470 340 329 399 569 594 324 337 444 485 544 556 401 425 421 431 528 561 497 519 461 473 486 411 470 483 497 507 554 598 375 395 494 518 430 431 1319 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 33 WEEK 37 WEEK 41 WEEK 45 WEEK 49 WEEK 53 WEEK 57 WEEK 61 GROUP: FEMALE 2 - 0.5 PPM C92990 338 367 386 407 428 436 450 475 GROUP: FEMALE 3 - 2 PPM C92991 320 338 337 357 355 383 395 421 C92992 348 361 385 410 420 439 474 460 C92994 315 325 336 336 349 364 379 371 C92997 302 307 313 323 329 326 342 348 C92998 383 391 299 216 C92999 377 388 415 434 449 458 470 483 C93001 363 383 393 402 415 422 450 455 C93002 336 343 365 384 390 405 427 441 C93003 383 390 415 434 432 447 462 478 C93004 343 365 392 422 441 450 485 504 C93005 364 364 378 381 396 401 436 439 C93007 323 339 345 368 383 389 415 435 C93009 365 372 402 396 413 431 445 457 C93010 377 392 414 422 431 444 481 492 C93011 356 382 402 417 432 452 469 490 C93012 407 416 439 458 462 488 503 513 C93013 308 319 334 353 368 382 412 437 C93014 410 430 453 482 505 528 568 604 C93015 357 365 363 393 400 407 426 449 C93016 289 302 304 326 337 334 350 369 C93017 367 373 388 408 423 440 463 483 C93019 425 458 475 298 C93020 355 392 410 405 442 452 493 511 C93021 392 402 429 453 477 476 413 338 C93022 403 405 439 452 472 478 509 546 C93024 330 350 367 394 403 427 444 475 C93025 386 428 434 457 452 476 505 545 Covance 6329-183 3M T-6295 PAGE: 63 WEEK 65 WEEK 69 497 511 428 466 480 497 375 391 356 369 493 528 460 480 454 496 475 494 521 471 436 466 441 454 461 472 510 527 509 518 512 540 457 478 628 590 431 431 324 326 479 502 402 575 595 483 491 562 576 1320 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 33 WEEK 37 WEEK 41 WEEK 45 WEEK 49 WEEK 53 WEEK 57 WEEK 61 GROUP: FEMALE 3 - 2 PPM C93026 320 331 350 356 363 364 382 393 C93027 329 340 354 367 384 384 414 435 C93028 351 350 366 383 391 405 411 436 C93029 310 320 328 336 346 355 364 383 C93030 382 410 421 447 468 486 510 516 C93032 361 384 400 415 433 443 460 493 C93033 384 395 398 376 371 339 307 308 C93034 341 342 355 357 365 380 389 412 C93036 393 428 450 478 505 525 549 575 C93037 326 354 368 402 429 459 463 486 C93038 305 312 334 349 363 386 401 425 C93039 379 386 408 440 459 478 510 524 C93040 285 291 298 313 323 328 333 347 C93041 399 416 435 458 461 484 489 507 C93042 427 453 478 521 546 567 597 638 C93043 388 400 414 431 455 473 496 523 C93044 312 328 337 358 370 391 412 387 C93046 360 381 383 397 404 423 444 464 C93047 480 481 522 563 571 601 650 670 C93048 291 302 306 324 324 329 346 376 C93049 325 341 361 371 388 387 412 431 C93050 335 344 364 393 407 422 451 467 GROUP: FEMALE 4 - 5 PPM C93052 357 370 404 417 444 467 492 513 C93053 398 414 431 453 474 495 508 466 C93054 313 325 346 360 382 386 415 415 C93055 395 387 409 442 479 508 532 550 C93056 351 358 367 380 386 392 413 424 C93057 321 323 341 351 361 368 383 399 Covance 6329-183 3M T-6295 PAGE: 64 WEEK 65 WEEK 69 391 405 462 476 447 463 394 410 536 557 513 519 289 416 419 615 649 445 471 534 540 558 347 364 521 524 659 647 536 556 339 466 482 697 739 379 396 392 314 474 497 484 433 350 390 392 585 609 443 461 400 418 1321 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 33 WEEK 37 WEEK 41 WEEK 45 WEEK 49 WEEK 53 WEEK 57 WEEK 61 GROUP: FEMALE 4 - 5 PPM C93059 342 370 372 392 407 413 438 461 C93060 332 332 350 376 374 391 415 439 C93061 404 410 427 453 470 493 522 528 C93062 313 328 337 349 356 365 378 393 C93063 333 349 358 377 388 397 408 422 C93065 274 289 298 308 319 333 350 366 C93066 365 367 391 404 419 443 454 484 C93068 338 351 365 377 385 396 420 424 C93069 426 432 434 456 463 484 503 511 C93072 373 373 382 401 418 416 434 444 C93073 331 340 338 349 371 405 422 442 C93074 416 432 453 410 472 506 553 578 C93076 359 386 401 429 430 428 470 495 C93077 387 398 413 434 440 450 427 445 C93078 327 340 350 361 368 381 423 441 C93079 370 385 393 440 445 486 515 526 C93080 344 352 365 380 390 408 424 427 C93081 319 328 341 350 367 391 428 436 C93082 400 404 412 429 436 437 446 463 C93083 340 353 368 387 397 427 442 454 C93086 348 354 369 382 387 408 436 446 C93087 295 303 294 323 327 335 360 372 C93088 342 342 349 360 369 387 404 412 C93089 330 339 356 363 372 380 396 407 C93090 301 302 314 344 341 356 372 399 C93091 368 390 400 420 432 449 472 501 C93092 326 337 347 356 358 367 393 406 C93093 389 409 432 445 463 473 492 521 C93094 330 354 368 379 391 410 435 455 C93095 322 333 340 345 353 366 374 389 C93097 338 344 356 361 378 395 394 407 Covance 6329-183 3M T-6295 PAGE: 65 WEEK 65 WEEK 69 473 489 424 466 534 555 393 406 412 435 371 375 463 463 419 435 522 534 454 464 431 393 586 607 504 557 444 483 441 465 540 578 433 445 429 458 466 489 462 493 466 487 369 388 412 376 397 419 408 421 516 549 397 429 512 510 475 488 393 421 412 437 1322 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 33 WEEK 37 WEEK 41 WEEK 45 WEEK 49 WEEK 53 WEEK 57 WEEK 61 GROUP: FEMALE 4 - 5 PPM C93098 324 364 357 365 388 382 409 434 C93099 379 400 414 439 453 478 506 542 C93100 339 341 366 391 411 418 444 476 C93101 310 321 332 351 373 376 399 418 C93102 378 390 404 428 439 451 476 488 C93103 352 396 424 439 474 476 495 512 C93104 481 504 498 532 536 535 562 574 C93105 332 352 362 384 391 405 438 449 C93106 316 327 344 378 369 374 399 437 C93107 333 336 342 354 357 364 371 367 C93108 311 317 321 332 348 C93110 542 542 573 582 611 614 638 641 GROUP: FEMALE 5 - 20 PPM C93112 342 350 353 362 362 372 C93113 288 290 312 321 330 344 359 371 C93115 266 284 306 322 335 344 377 388 C93116 289 296 302 307 320 317 C93117 348 362 387 400 413 422 C93118 307 308 304 318 332 329 336 332 C93119 263 271 288 296 306 321 331 334 C93120 277 285 300 309 317 314 C93121 255 259 264 267 277 275 284 286 C93122 391 402 420 438 468 473 493 501 C93124 294 291 296 307 315 321 332 340 C93125 258 264 277 280 283 281 294 306 C93126 297 304 314 326 345 346 294 C93128 306 301 317 327 332 333 345 349 C93129 301 311 311 325 340 335 356 365 C93130 345 343 372 373 401 418 Covance 6329-183 3M T-6295 PAGE: 66 WEEK 65 WEEK 69 443 447 542 549 470 485 424 422 484 503 540 558 549 522 449 471 442 467 373 395 643 665 372 385 403 430 317 358 271 297 291 303 506 541 343 349 297 312 354 372 368 392 1323 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 33 WEEK 37 WEEK 41 WEEK 45 WEEK 49 WEEK 53 WEEK 57 WEEK 61 GROUP: FEMALE 5 - 20 PPM C93131 341 353 366 383 399 413 435 446 C93132 341 359 364 383 399 382 399 403 C93133 291 288 333 339 338 316 362 354 C93134 329 345 353 365 379 411 447 469 C93135 317 321 330 354 351 351 379 387 C93136 319 325 336 349 353 363 C93137 290 294 304 310 346 347 365 389 C93138 315 327 335 345 359 387 C93139 274 281 297 311 316 315 332 344 C93140 385 413 436 455 486 493 509 519 C93141 342 360 373 387 403 416 432 435 C93145 274 280 280 295 310 309 322 338 C93146 263 261 271 277 280 282 299 302 C93147 287 291 293 309 309 315 C93148 353 359 366 362 386 393 410 419 C93149 305 313 319 346 366 369 386 401 C93150 318 321 322 328 358 356 369 372 C93151 294 312 310 316 314 319 327 330 C93152 305 314 316 328 332 342 348 329 C93153 278 283 297 307 321 335 351 358 C93154 294 323 332 339 349 342 C93156 292 303 305 320 325 320 335 335 C93157 294 309 324 351 347 366 390 404 C93158 322 321 333 340 355 379 392 403 C93160 392 419 441 471 487 481 502 507 C93161 329 367 354 386 402 433 453 473 C93162 326 338 339 339 357 370 376 381 C93163 292 303 314 323 333 341 C93164 281 288 289 296 306 318 328 335 C93165 305 316 321 336 342 348 360 375 C93166 355 356 380 373 388 409 420 430 Covance 6329-183 3M T-6295 PAGE: 67 WEEK 65 WEEK 69 463 479 415 427 376 389 543 397 414 399 428 346 361 525 534 458 479 332 332 297 312 420 445 404 429 380 386 329 340 270 368 387 340 351 405 421 405 431 531 529 502 509 380 390 339 358 379 388 451 453 1324 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 33 WEEK 37 WEEK 41 WEEK 45 WEEK 49 WEEK 53 WEEK 57 WEEK 61 GROUP: FEMALE 5 - 20 PPM C93167 370 379 C93168 287 287 305 302 310 333 348 354 C93170 326 338 343 359 369 380 395 403 C93171 329 342 351 362 370 384 C93172 328 334 341 354 372 370 388 391 C93173 302 308 318 335 341 348 367 371 C93174 326 332 338 346 383 396 388 415 C93175 282 289 289 299 296 302 306 309 C93176 335 350 357 372 380 396 417 416 C93177 275 277 278 290 295 296 296 304 C93178 292 306 304 313 326 334 344 349 C93179 275 277 285 292 299 295 303 312 C93180 346 358 371 383 399 403 430 512 GROUP: FEMALE 6 - 20 PPM C93181 304 308 313 315 331 354 359 361 C93182 282 287 288 299 308 317 345 360 C93183 356 386 382 387 419 437 456 491 C93184 267 267 282 289 301 306 334 354 C93185 317 328 323 326 341 351 360 382 C93186 268 283 291 306 324 345 384 415 C93187 376 378 395 404 417 432 457 477 C93188 347 365 373 409 422 444 486 521 C93189 263 252 258 275 302 314 322 348 C93190 328 340 351 368 366 375 408 435 C93191 358 390 387 416 425 434 465 487 C93192 294 285 297 308 324 320 333 374 C93193 372 387 420 454 481 497 531 560 C93194 339 353 363 370 372 384 405 420 C93195 325 337 347 369 384 390 428 456 Covance 6329-183 3M T-6295 PAGE: 68 WEEK 65 WEEK 69 373 380 405 415 406 407 373 387 424 430 307 316 427 440 310 309 355 359 315 320 377 388 379 398 500 528 364 397 408 436 420 444 500 521 564 594 352 355 458 480 504 529 388 405 606 631 422 445 481 501 1325 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 33 WEEK 37 WEEK 41 WEEK 45 WEEK 49 WEEK 53 WEEK 57 WEEK 61 GROUP: FEMALE 6 - 20 PPM C93196 281 286 290 312 307 322 348 378 C93197 244 243 265 264 272 271 275 286 C93198 323 336 350 371 388 397 430 456 C93199 302 316 324 328 335 341 371 383 C93200 280 302 304 318 315 324 348 364 C93201 275 282 283 295 296 299 320 336 C93202 275 283 285 287 291 292 311 346 C93203 346 359 372 392 401 412 467 489 C93204 297 305 318 329 348 360 393 408 C93205 317 318 330 338 342 348 354 384 C93206 309 306 319 329 342 342 382 409 C93207 280 289 206 C93208 286 311 312 339 350 359 396 433 C93209 294 295 303 320 334 344 379 412 C93210 348 364 380 397 419 443 452 504 C93211 252 260 267 264 274 272 276 298 C93212 331 358 376 403 420 424 471 521 C93213 308 310 323 321 313 332 361 382 C93214 301 316 327 343 355 366 405 429 C93215 316 324 331 348 354 368 414 447 C93216 326 341 355 370 375 388 421 439 C93217 356 367 381 377 388 382 404 427 C93218 285 294 304 318 326 339 366 376 C93219 360 349 373 392 393 399 435 482 C93220 398 385 409 440 436 441 478 496 Covance 6329-183 3M T-6295 PAGE: 69 WEEK 65 WEEK 69 390 405 285 305 470 489 316 371 392 335 352 333 349 493 519 419 439 399 407 447 479 474 515 451 489 529 569 311 340 517 560 369 389 444 479 468 491 468 495 445 477 400 417 504 540 512 536 1326 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 73 WEEK 77 WEEK 81 WEEK 85 WEEK 89 WEEK 93 WEEK 97 WEEK 101 GROUP: C92502 C92503 C92505 C92508 C92510 C92513 C92514 C92515 C92518 C92519 C92520 C92522 C92527 C92530 C92533 C92535 C92536 C92537 C92538 C92539 C92541 C92542 C92543 C92544 C92545 C92547 C92548 C92549 C92550 C92551 C92552 C92554 MALE 1 - 0 PPM 640 497 771 759 729 742 659 498 757 764 743 746 962 972 749 740 891 902 720 739 644 603 818 828 841 842 790 692 928 940 823 827 832 842 1063 1077 1155 951 939 941 958 754 766 733 745 660 672 935 942 850 857 758 676 680 694 954 979 743 742 903 902 697 708 728 744 774 755 986 751 922 565 825 709 947 840 1096 944 971 772 747 672 952 836 570 987 743 903 717 687 756 781 765 1005 764 928 838 904 843 1111 918 989 794 770 675 998 790 1001 755 885 735 612 745 780 757 970 765 920 824 842 1100 839 981 798 772 680 1015 996 749 829 742 581 751 795 769 953 779 945 800 846 1101 780 1018 817 785 675 1008 1010 772 766 752 532 746 807 789 1015 781 814 723 596 1000 835 802 620 1018 765 689 736 478 707 806 786 1056 856 899 832 604 992 756 553 690 Covance 6329-183 3M T-6295 PAGE: 70 WEEK 105 445 679 808 789 1070 892 760 821 583 719 1327 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 73 WEEK 77 WEEK 81 WEEK 85 WEEK 89 WEEK 93 WEEK 97 WEEK 101 GROUP: MALE 1 - 0 PPM C92557 818 817 783 671 459 C92558 793 661 C92559 769 770 C92560 867 774 C92563 745 743 702 C92565 853 857 853 861 838 C92567 855 854 861 848 826 C92568 806 802 790 799 790 760 722 651 C92569 683 681 682 685 668 688 676 658 GROUP: MALE 2 - 0.5 PPM C92571 859 877 910 953 972 1007 926 662 C92572 863 854 858 830 826 792 724 514 C92573 854 818 752 C92576 948 946 982 936 945 928 803 C92577 974 967 982 943 952 977 951 938 C92578 973 964 932 776 C92579 820 759 698 557 C92580 808 632 C92581 972 973 947 954 907 833 688 593 C92582 675 676 693 701 714 744 731 586 C92583 754 749 766 737 740 735 729 686 C92586 529 C92587 840 823 816 652 C92588 685 678 674 665 668 670 631 528 C92589 831 813 782 726 693 632 517 C92590 843 839 836 859 868 876 877 897 C92591 811 803 808 822 827 830 C92592 890 883 879 895 C92594 765 764 760 770 798 806 Covance 6329-183 3M T-6295 PAGE: 71 WEEK 105 644 934 364 624 887 1328 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 73 WEEK 77 WEEK 81 WEEK 85 WEEK 89 WEEK 93 WEEK 97 WEEK 101 GROUP: MALE 2 - 0.5 PPM C92595 713 663 597 C92597 703 692 695 708 694 708 701 710 C92598 760 764 770 782 783 787 801 796 C92601 802 815 829 839 835 857 859 864 C92602 613 593 605 608 620 641 658 662 C92604 924 822 716 604 472 C92605 907 894 874 872 871 845 778 678 C92606 777 764 C92607 868 857 820 663 520 C92608 655 469 C92609 799 781 792 809 824 832 843 823 C92611 688 696 722 805 C92612 815 606 C92614 758 746 741 739 740 C92615 933 921 907 865 721 C92617 881 744 561 C92619 679 672 677 685 692 717 711 726 C92620 610 615 590 613 617 569 C92622 891 910 901 C92623 686 686 698 713 717 735 739 744 C92625 867 776 687 C92626 736 751 718 735 746 768 788 788 C92628 465 C92629 788 638 C92630 729 715 656 561 GROUP: MALE 3 - 2 PPM C92631 725 729 742 754 765 786 807 804 C92632 846 842 815 792 778 778 772 758 C92633 841 840 846 847 829 859 869 853 Covance 6329-183 3M T-6295 PAGE: 72 WEEK 105 698 781 853 668 732 741 788 808 871 1329 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 73 WEEK 77 WEEK 81 WEEK 85 WEEK 89 WEEK 93 WEEK 97 WEEK 101 GROUP: MALE 3 - 2 PPM C92634 825 828 841 862 C92635 756 605 C92636 875 870 894 908 928 937 937 C92637 702 707 718 726 729 711 673 605 C92638 980 917 849 702 575 C92639 725 582 628 489 C92641 740 727 731 743 708 717 712 748 C92642 789 783 780 784 786 798 814 829 C92643 670 666 666 680 681 688 695 720 C92644 705 696 703 692 712 738 744 748 C92647 780 768 C92648 706 675 630 524 C92649 685 682 689 697 701 726 711 724 C92651 702 572 380 C92653 927 932 945 957 962 965 968 953 C92654 743 652 C92655 871 858 845 844 824 710 C92656 857 844 829 813 751 616 461 C92657 782 774 782 792 787 804 806 833 C92658 868 865 878 867 888 844 C92659 835 802 777 769 767 779 795 737 C92660 622 623 614 640 662 644 663 667 C92661 725 684 614 422 429 C92663 940 955 966 983 975 994 C92664 744 608 CO CO CO C92665 936 934 928 C92666 645 C92667 895 670 C92669 843 832 838 854 875 889 918 923 C92670 422 C92671 776 772 762 803 812 790 Covance 6329-183 3M T-6295 PAGE: 73 WEEK 105 536 665 831 741 702 729 823 820 748 703 858 1330 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 73 WEEK 77 WEEK 81 WEEK 85 WEEK 89 WEEK 93 WEEK 97 WEEK 101 GROUP: MALE 3 - 2 PPM C92672 C92673 C92674 C92675 C92677 C92680 C92681 C92682 C92683 C92685 C92686 C92688 C92690 893 849 890 758 759 558 769 842 913 827 906 627 1042 903 853 900 771 752 555 728 840 923 725 922 597 1035 GROUP: MALE 4 - 5 PPM C92691 C92693 C92694 C92695 C92696 C92698 C92699 C92700 C92702 C92704 C92705 C92706 C92707 C92708 C92709 785 783 867 727 744 1006 631 604 729 853 824 1021 719 704 649 771 773 867 720 729 1021 626 591 745 841 827 1015 719 697 606 912 862 919 762 737 524 669 845 939 856 631 1027 766 775 876 725 742 1016 635 598 760 848 828 973 717 707 573 917 885 933 766 736 583 846 947 629 1025 755 771 893 725 750 1015 648 603 774 854 832 931 729 707 502 911 877 934 762 760 868 953 620 1047 763 793 916 739 778 1041 673 616 801 874 847 931 592 729 467 903 845 950 751 767 913 998 516 1043 769 820 936 763 804 1077 680 629 809 905 867 943 765 960 699 760 923 992 413 1052 771 811 960 772 815 1091 657 637 814 908 866 888 782 982 564 941 1002 1076 771 750 975 779 824 1060 678 624 751 916 878 775 Covance 6329-183 3M T-6295 PAGE: 74 WEEK 105 968 931 947 1073 768 751 968 767 824 797 674 568 635 903 899 755 1331 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 73 WEEK 77 WEEK 81 WEEK 85 WEEK 89 WEEK 93 WEEK 97 WEEK 101 GROUP: MALE 4 - 5 PPM C92711 804 800 809 819 727 C92712 921 877 768 611 C92716 548 503 506 503 519 498 517 481 C92717 828 831 823 831 831 832 760 698 C92720 886 908 1019 C92721 695 702 C92723 861 855 C92724 958 953 946 950 958 974 1004 995 C92726 733 735 729 730 751 762 690 739 C92727 843 843 815 810 776 C92729 698 631 C92730 814 810 813 834 837 838 780 700 C92732 834 825 821 C92733 712 693 683 569 418 C92735 791 796 796 811 816 834 829 826 C92736 862 865 873 876 888 880 872 901 C92737 822 825 837 824 708 C92738 744 733 718 673 649 C92739 686 693 695 712 734 754 770 781 C92740 780 739 771 780 808 827 839 876 C92741 744 757 738 704 540 C92742 924 938 941 926 919 950 958 963 C92743 1046 1075 1089 C92745 1102 1120 1040 872 703 C92746 886 895 C92747 811 803 815 827 750 778 785 761 C92748 811 817 813 818 821 830 758 715 C92749 744 750 745 759 781 796 818 818 C92750 826 819 823 833 838 843 838 819 Covance 6329-183 3M T-6295 PAGE: 75 WEEK 105 495 649 997 776 588 829 897 779 887 931 748 620 702 1332 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 73 WEEK 77 WEEK 81 WEEK 85 WEEK 89 WEEK 93 WEEK 97 WEEK 101 GROUP: C92753 C92756 C92757 C92760 C92761 C92762 C92763 C92764 C92766 C92767 C92768 C92769 C92770 C92771 C92773 C92774 C92775 C92776 C92780 C92781 C92782 C92783 C92784 C92785 C92786 C92788 C92791 C92792 C92797 C92800 C92802 MALE 5 - 20 PPM 736 898 702 551 649 832 1121 594 663 1269 827 832 937 932 559 778 901 768 672 517 893 945 844 780 808 792 797 787 779 682 897 744 901 721 638 822 1113 650 1200 831 824 929 929 558 778 909 763 669 379 911 861 745 784 806 803 795 781 778 682 904 754 893 723 643 803 1118 663 1168 838 828 845 763 579 784 910 744 671 926 792 809 799 803 775 774 677 907 771 770 753 612 786 1140 673 1178 852 833 886 565 786 916 754 690 956 809 814 828 820 795 778 683 933 807 765 589 801 1120 652 1223 849 798 890 555 808 931 757 697 976 811 835 836 825 788 828 696 939 824 753 579 807 932 673 1201 926 788 908 567 811 920 767 689 984 819 840 852 837 798 965 689 938 843 742 574 791 664 1100 777 905 569 810 886 778 667 1013 755 852 874 845 765 688 981 810 701 576 736 655 705 689 580 821 820 788 611 1026 864 892 850 751 678 1014 Covance 6329-183 3M T-6295 PAGE: 76 WEEK 105 776 608 564 677 597 797 673 785 543 1017 872 889 859 714 638 988 1333 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 73 WEEK 77 WEEK 81 WEEK 85 WEEK 89 WEEK 93 WEEK 97 WEEK 101 GROUP: MALE 5 - 2 0 PPM C92803 704 704 702 705 706 720 724 734 C92804 836 848 841 878 886 892 888 938 C92806 728 763 763 776 785 776 720 C92807 849 864 809 702 C92808 626 625 630 632 644 646 635 652 C92809 738 759 769 785 798 839 885 901 C92810 836 855 847 865 785 667 422 C92813 797 818 788 789 751 740 615 C92814 556 551 567 567 571 588 591 609 C92815 643 645 654 686 690 705 713 770 C92818 733 726 730 733 731 750 752 716 C92819 712 721 722 735 745 775 787 769 GROUP: MALE 6 - 2 0 PPM C92821 693 718 743 775 788 806 800 799 C92822 715 613 496 C92823 758 761 790 792 822 822 850 847 C92824 751 613 721 611 651 625 C92825 1119 1112 1068 1048 994 954 C92827 784 778 796 809 813 834 841 849 C92828 829 832 840 C92829 923 925 912 847 793 856 902 892 C92830 638 604 564 534 489 463 448 C92831 735 712 724 622 C92833 1028 1042 1039 1044 1019 917 920 786 C92834 782 778 767 760 751 757 754 719 C92835 687 C92836 860 850 874 869 877 C92839 1116 1083 1091 1023 904 C92840 727 717 725 735 747 773 784 676 Covance 6329-183 3M T-6295 PAGE: 77 WEEK 105 753 951 643 872 621 769 755 821 835 820 765 836 696 1334 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 73 WEEK 77 WEEK 81 WEEK 85 WEEK 89 WEEK 93 WEEK 97 WEEK 101 GROUP: MALE 6 - 20 PPM C92842 852 851 855 847 827 C92843 978 979 C92844 553 564 542 C92845 684 679 686 680 687 704 C92846 780 768 756 747 656 701 662 618 C92847 978 978 958 946 923 809 644 C92848 502 503 510 466 475 481 463 471 C92850 686 705 733 740 738 738 671 654 C92851 832 844 852 850 823 828 819 815 C92853 824 845 860 874 870 899 907 863 C92854 916 930 943 956 915 967 968 925 C92856 682 677 675 675 587 652 663 648 C92857 870 868 716 C92858 865 877 879 898 879 764 534 C92860 697 708 728 733 730 751 639 GROUP: FEMALE 1 - 0 PPM C92863 505 500 497 C92866 466 471 488 500 506 518 510 467 C92867 513 531 552 567 592 597 603 611 C92868 469 474 486 495 521 535 552 580 C92869 400 404 423 433 452 450 454 462 C92870 505 524 536 571 590 570 535 493 C92871 561 568 600 626 642 672 687 696 C92872 458 480 498 495 512 C92873 410 415 443 447 458 459 474 487 C92874 525 540 541 568 593 610 608 628 C92875 440 370 C92877 507 503 510 519 533 524 533 531 C92878 457 461 520 Covance 6329-183 3M T-6295 PAGE: 78 WEEK 105 379 511 827 770 650 386 621 580 471 673 470 578 519 1335 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 73 WEEK 77 WEEK 81 WEEK 85 WEEK 89 WEEK 93 WEEK 97 WEEK 101 GROUP: FEMALE 1 - 0 PPM C92879 511 511 433 376 361 312 C92881 490 473 479 498 499 497 511 517 C92882 458 464 479 482 507 496 480 484 C92883 486 493 502 470 C92884 429 416 406 367 361 381 C92885 521 529 535 548 558 555 536 524 C92886 396 443 681 C92888 683 676 C92890 527 545 553 549 546 541 555 560 C92891 401 396 380 353 C92892 678 703 721 749 744 765 759 759 C92893 368 372 380 408 C92895 442 451 459 458 462 475 477 485 C92896 527 517 533 530 548 547 557 556 C92897 384 400 435 424 460 474 463 400 C92901 447 342 C92902 805 817 815 807 C92904 428 432 448 468 476 469 477 479 C92906 399 415 415 415 422 410 401 418 C92907 420 427 445 459 476 493 492 487 C92908 578 587 601 599 508 368 C92909 435 444 442 451 444 459 458 466 C92911 380 381 384 373 385 368 381 395 C92912 658 677 696 693 715 713 739 749 C92913 405 406 419 425 440 454 461 467 C92915 539 547 552 563 560 422 C92916 705 708 678 684 569 479 C92918 376 C92924 499 497 491 483 C92925 598 613 633 645 608 558 476 593 C92927 415 419 420 429 448 440 440 441 Covance 6329-183 3M T-6295 PAGE: 79 WEEK 105 522 469 489 540 726 497 563 322 371 497 482 385 735 438 599 455 1336 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T- 6295) IN RATS ANIMAL NUMBER WEEK 73 WEEK 77 WEEK 81 WEEK 85 WEEK 89 WEEK 93 WEEK 97 WEEK 101 GROUP: FEMALE 1 - 0 PPM C92928 606 620 585 GROUP: FEMALE 2 - 0.5 PPM C92933 602 598 611 622 624 C92934 469 481 477 403 C92935 638 627 559 472 C92936 415 421 431 437 440 441 432 423 C92938 628 642 666 659 682 696 719 735 C92940 458 473 511 558 C92943 612 637 655 595 515 391 353 C92946 492 514 509 534 552 566 577 599 C92947 455 460 475 483 476 413 C92948 592 595 600 608 612 573 449 C92951 391 393 404 411 422 429 407 413 C92953 391 406 411 419 435 441 453 454 C92954 480 501 517 527 564 566 576 464 C92955 612 612 572 518 429 C92956 588 592 614 626 650 663 678 624 C92957 458 465 469 471 493 501 513 519 C92958 442 454 449 408 358 C92959 469 492 503 511 547 552 460 C92960 546 564 591 616 634 604 653 674 C92961 738 744 729 779 844 844 747 C92963 379 373 372 374 378 353 C92964 485 464 454 473 498 487 495 516 C92965 455 452 305 C92970 604 628 646 654 661 633 597 487 C92971 347 354 406 473 584 C92972 481 485 513 512 501 394 C92973 571 592 606 634 652 655 687 701 Covance 6329-183 3M T-6295 PAGE: 80 WEEK 105 343 688 592 468 566 704 529 367 644 1337 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 73 WEEK 77 WEEK 81 WEEK 85 WEEK 89 WEEK 93 WEEK 97 WEEK 101 GROUP: FEMALE 2 - 0.5 PPM C92974 424 425 440 440 462 450 452 402 C92975 440 448 461 487 503 517 515 496 C92976 611 679 738 729 733 715 661 529 C92977 525 530 552 549 568 570 571 C92979 477 491 504 522 409 C92983 497 508 528 542 569 588 595 551 C92984 531 546 566 572 590 601 597 578 C92985 642 681 724 723 743 708 608 C92986 395 402 424 432 458 482 499 503 C92988 526 543 540 562 571 591 613 606 C92989 444 451 446 C92990 500 519 538 550 550 540 GROUP: FEMALE 3 - 2 PPM C92991 472 498 527 549 557 576 589 595 C92992 512 510 523 C92994 401 417 364 C92997 373 364 354 C92999 500 447 415 333 323 C93001 491 471 402 C93002 502 456 385 C93003 492 504 507 528 561 561 570 537 C93004 393 C93005 480 481 381 349 387 C93007 453 457 470 482 480 511 516 531 C93009 483 477 478 464 443 C93010 558 574 593 575 471 C93011 546 547 559 583 605 624 639 657 C93012 596 605 595 C93013 499 435 364 299 Covance 6329-183 3M T-6295 PAGE: 81 WEEK 105 363 531 402 544 489 617 1338 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 73 WEEK 77 WEEK 81 WEEK 85 WEEK 89 WEEK 93 WEEK 97 W1E01EK GROUP: FEMALE 3 - 2 PPM C93014 489 C93015 478 485 497 503 515 563 495 485 C93016 293 305 278 C93017 512 524 524 534 519 C93022 593 632 647 677 687 681 670 649 C93024 510 507 433 370 C93025 576 584 598 618 632 656 661 628 C93026 412 425 416 415 428 411 397 375 C93027 500 505 543 530 423 C93028 479 494 490 466 427 C93030 581 591 611 619 617 581 C93032 504 388 C93034 412 404 432 C93036 693 745 771 752 C93039 566 579 600 603 597 594 612 618 C93040 372 373 386 384 384 348 359 333 C93041 533 533 439 C93042 673 677 653 590 515 436 C93043 572 583 597 608 610 608 633 C93046 468 490 888C93047 803 829 818 849 878 918 913 C93048 401 411 419 428 433 445 446 474 C93050 504 522 536 535 546 550 508 GROUP: FEMALE 4 - 5 PPM C93055 634 524 C93056 475 495 519 537 552 527 432 C93057 429 431 438 458 458 428 440 C93059 496 510 520 541 557 584 596 615 C93060 465 473 480 480 502 508 510 480 Covance 6329-183 3M T-6295 PAGE: 82 WEEK 105 621 498 1339 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 73 WEEK 77 WEEK 81 WEEK 85 WEEK 89 WEEK 93 WEEK 97 WEEK 101 GROUP: FEMALE 4 - 5 PPM C93061 513 412 C93062 411 405 422 429 428 430 442 432 C93063 429 448 465 460 482 504 526 528 C93065 367 298 309 292 324 350 C93066 477 481 494 493 528 532 C93068 447 456 449 440 450 430 431 382 C93069 558 565 570 589 611 642 716 685 C93072 472 463 480 481 474 510 519 506 C93073 289 C93074 608 622 522 C93076 598 616 621 670 674 570 C93077 511 521 550 588 604 627 640 627 C93078 464 479 486 486 510 534 592 601 C93079 578 588 600 614 623 619 629 648 C93080 469 472 473 500 516 518 530 537 C93081 449 458 450 454 457 482 491 479 C93082 487 497 509 523 542 569 507 C93083 503 511 536 566 565 575 597 556 C93086 513 449 382 346 341 C93087 402 402 422 425 442 467 437 C93088 339 376 C93089 415 408 412 418 417 375 C93090 428 442 453 456 475 498 512 513 C93091 553 510 C93092 370 312 C93093 516 521 521 498 480 475 C93094 521 524 533 559 552 536 C93095 421 430 444 449 430 431 420 440 C93097 435 442 439 440 424 444 412 C93098 451 470 478 481 482 500 517 519 C93099 560 566 580 571 557 537 Covance 6329-183 3M T-6295 PAGE: 83 WEEK 105 434 529 521 621 643 623 537 484 508 453 290 490 1340 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 73 WEEK 77 WEEK 81 WEEK 85 WEEK 89 WEEK 93 WEEK 97 WEEK 101 GROUP: FEMALE 4 - 5 PPM C93100 498 514 535 544 535 C93101 440 451 448 450 437 462 484 479 C93102 499 506 510 521 532 544 554 545 C93103 575 608 634 615 635 644 552 C93104 460 348 281 C93105 408 411 394 310 329 C93106 476 498 502 515 513 518 C93107 397 392 403 418 421 437 468 474 C93110 686 666 660 620 483 GROUP: FEMALE 5 - 20 PPM C93113 408 425 442 446 472 507 525 546 C93115 434 463 482 507 518 541 562 528 C93118 363 407 379 433 422 392 C93119 313 330 341 339 361 C93121 269 215 C93122 599 600 605 606 543 C93124 359 357 342 336 323 356 354 390 C93125 314 313 308 308 307 298 292 276 C93128 378 380 398 419 430 436 446 C93129 396 396 410 425 429 396 335 C93131 485 494 482 487 503 467 C93132 437 455 458 485 493 472 445 445 C93133 395 404 402 414 413 416 424 426 C93135 365 391 368 381 398 416 435 448 C93137 455 460 507 494 504 496 482 467 C93139 364 363 374 373 366 352 345 332 C93140 542 537 545 559 563 571 565 548 C93141 486 488 512 534 537 545 530 C93145 328 Covance 6329-183 3M T-6295 PAGE: 84 WEEK 105 490 562 494 580 507 432 301 265 425 473 446 329 514 1341 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 73 WEEK 77 WEEK 81 WEEK 85 WEEK 89 WEEK 93 WEEK 97 WEEK 101 GROUP: FEMALE 5 - 20 PPM C93146 323 330 334 342 353 352 360 373 C93148 462 462 479 492 505 C93149 434 429 441 432 449 447 472 413 C93150 384 378 352 360 341 C93151 335 335 336 339 345 377 401 456 C93153 407 410 403 416 421 410 486 452 C93156 335 262 362 345 C93157 440 458 469 485 499 513 552 572 C93158 439 429 438 457 455 465 459 464 C93160 408 C93161 543 563 557 583 583 652 687 700 C93162 408 411 411 413 433 439 452 447 C93164 367 381 390 410 416 430 457 464 C93165 399 415 421 426 424 438 463 462 C93166 461 471 464 486 476 496 509 522 C93168 381 387 391 399 395 400 419 413 C93170 424 427 441 446 433 383 C93172 341 261 C93173 396 407 401 385 437 473 504 492 C93174 433 445 458 461 476 485 485 494 C93175 320 319 318 306 316 C93176 449 450 424 377 C93177 315 320 320 322 327 331 342 346 C93178 367 379 377 381 388 396 403 396 C93179 322 318 325 308 307 316 331 326 GROUP: FEMALE 6 - 20 PPM C93181 405 407 419 431 436 448 461 470 C93182 434 467 491 508 523 538 560 568 C93183 547 578 600 621 646 678 707 749 Covance 6329-183 3M T-6295 PAGE: 85 WEEK 105 356 338 526 463 583 485 729 429 476 464 511 407 495 355 394 331 472 559 804 1342 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 73 WEEK 77 WEEK 81 WEEK 85 WEEK 89 WEEK 93 WEEK 97 WEEK 101 GROUP: FEMALE 6 - 20 PPM C93184 432 443 465 490 518 544 559 580 C93185 458 468 480 503 506 528 554 549 C93186 457 C93187 535 545 553 567 496 566 557 491 C93188 617 645 666 694 715 720 721 687 C93189 380 380 386 301 C93190 507 518 526 546 543 523 441 340 C93191 546 553 563 596 581 C93192 433 464 467 496 519 537 569 570 C93193 632 C93194 450 450 424 358 346 C93196 432 432 433 413 C93197 308 305 324 324 324 350 356 362 C93198 511 519 536 547 544 569 574 579 C93200 415 421 434 454 485 474 C93201 365 389 399 411 421 448 449 453 C93202 384 382 390 419 427 441 462 459 C93203 544 526 509 460 423 C93204 428 438 442 470 484 C93205 430 451 472 486 501 509 540 520 C93206 504 525 553 566 580 594 503 388 C93208 548 571 575 C93209 505 540 541 576 585 584 614 634 C93210 593 608 619 658 700 677 604 471 C93211 361 357 376 404 409 426 424 425 C93212 580 568 519 C93213 410 376 310 C93214 512 531 550 572 611 620 635 C93215 511 501 416 335 C93216 505 531 541 535 551 549 578 579 C93217 503 517 529 583 604 Covance 6329-183 3M T-6295 PAGE: 86 WEEK 105 583 569 709 330 551 369 590 472 472 463 638 337 383 577 1343 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 73 WEEK 77 WEEK 81 WEEK 85 WEEK 89 WEEK 93 WEEK 97 WEEK 101 GROUP: FEMALE 6 - 20 PPM C93218 428 442 453 458 470 C93219 560 577 588 622 651 683 733 763 C93220 553 568 584 595 599 608 618 624 Covance 6329-183 3M T-6295 PAGE: 87 WEEK 105 794 620 1344 APPENDIX 4 Covance 6329-183 3M T-6295 Individual Food Consumption Data (g) Individual Test Material Consumption Data (mg/kg/day) 1345 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 GROUP: C92501 C92502 C92503 C92504 C92505 C92506 C92507 C92508 C92509 C92510 C92511 C92512 C92513 C92514 C92515 C92516 C92517 C92518 C92519 C92520 C92521 C92522 C92523 C92524 C92525 C92526 C92527 C92528 C92529 C92530 C92531 MALE 1 - 0 PPM 178.00 164.00 166.00 169.00 163.00 160.00 169.00 160.00 157.00 177.00 179.00 166.00 165.00 179.00 151.00 156.00 180.00 167.00 154.00 154.00 164.00 164.00 148.00 190.00 166.00 181.00 168.00 151.00 159.00 SPILLED 168.00 203.00 166.00 178.00 181.00 SPILLED 171.00 196.00 165.00 164.00 SPILLED 192.00 179.00 SPILLED 200.00 164.00 168.00 192.00 191.00 160.00 181.00 170.00 177.00 158.00 200.00 169.00 186.00 SPILLED 159.00 179.00 SPILLED 181.00 212.00 180.00 191.00 177.00 175.00 182.00 191.00 177.00 172.00 205.00 202.00 181.00 191.00 205.00 174.00 176.00 202.00 183.00 173.00 196.00 188.00 175.00 168.00 199.00 182.00 196.00 198.00 170.00 175.00 SPILLED 204.00 217.00 185.00 203.00 184.00 SPILLED NOT TAKEN 195.00 174.00 180.00 218.00 210.00 181.00 SPILLED 211.00 181.00 180.00 213.00 196.00 175.00 187.00 186.00 179.00 172.00 NOT TAKEN 187.00 NOT TAKEN 195.00 183.00 NOT TAKEN SPILLED SPILLED 214.00 178.00 192.00 187.00 SPILLED 189.00 175.00 174.00 213.00 SPILLED 187.00 SPILLED 203.00 176.00 176.00 218.00 196.00 171.00 189.00 203.00 169.00 173.00 190.00 188.00 180.00 223.00 206.00 218.00 179.00 SPILLED 186.00 201.00 189.00 182.00 169.00 208.00 235.00 196.00 SPILLED 208.00 175.00 179.00 215.00 199.00 180.00 190.00 198.00 180.00 178.00 189.00 189.00 190.00 SPILLED SPILLED 218.00 176.00 194.00 203.00 194.00 185.00 182.00 173.00 209.00 SPILLED 190.00 SPILLED 207.00 185.00 171.00 215.00 200.00 176.00 193.00 191.00 165.00 175.00 181.00 185.00 192.00 SPILLED SPILLED 219.00 180.00 192.00 199.00 SPILLED 181.00 174.00 167.00 210.00 SPILLED 180.00 SPILLED 204.00 184.00 165.00 209.00 198.00 169.00 SPILLED SPILLED 171.00 176.00 183.00 186.00 176.00 SPILLED SPILLED Covance 6329-183 3M T-6295 PAGE: 1 WEEK 9 WEEK 10 218.00 178.00 197.00 193.00 184.00 178.00 174.00 178.00 199.00 231.00 183.00 191.00 206.00 182.00 171.00 210.00 205.00 169.00 196.00 185.00 167.00 178.00 183.00 196.00 173.00 SPILLED SPILLED 222.00 181.00 191.00 194.00 SPILLED 184.00 177.00 174.00 193.00 SPILLED 192.00 185.00 213.00 182.00 166.00 207.00 211.00 172.00 187.00 197.00 169.00 169.00 181.00 190.00 SPILLED SPILLED SPILLED 1346 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 GROUP: C92532 C92533 C92534 C92535 C92536 C92537 C92538 C92539 C92540 C92541 C92542 C92543 C92544 C92545 C92546 C92547 C92548 C92549 C92550 C92551 C92552 C92553 C92554 C92555 C92556 C92557 C92558 C92559 C92560 C92561 C92562 MALE 1 - 0 PPM 200.00 193.00 181.00 173.00 165.00 177.00 160.00 181.00 176.00 173.00 171.00 169.00 166.00 177.00 163.00 178.00 171.00 149.00 146.00 155.00 168.00 136.00 150.00 161.00 157.00 156.00 171.00 181.00 161.00 143.00 219.00 215.00 206.00 170.00 185.00 180.00 190.00 175.00 188.00 190.00 183.00 185.00 171.00 SPILLED 177.00 171.00 195.00 169.00 157.00 161.00 166.00 177.00 145.00 154.00 180.00 185.00 170.00 190.00 183.00 172.00 158.00 188.00 SPILLED 206.00 178.00 194.00 187.00 202.00 183.00 191.00 190.00 195.00 184.00 167.00 196.00 176.00 172.00 199.00 168.00 164.00 175.00 159.00 176.00 157.00 153.00 183.00 181.00 178.00 202.00 183.00 176.00 166.00 186.00 234.00 206.00 191.00 205.00 198.00 208.00 191.00 204.00 204.00 198.00 173.00 159.00 189.00 184.00 NOT TAKEN 210.00 162.00 166.00 188.00 165.00 195.00 SPILLED 162.00 242.00 189.00 187.00 202.00 182.00 176.00 180.00 183.00 241.00 211.00 186.00 202.00 197.00 212.00 195.00 202.00 206.00 193.00 196.00 178.00 182.00 190.00 209.00 178.00 170.00 195.00 160.00 189.00 152.00 156.00 186.00 180.00 188.00 204.00 188.00 175.00 179.00 182.00 SPILLED SPILLED 192.00 SPILLED 197.00 219.00 197.00 207.00 210.00 193.00 184.00 178.00 184.00 196.00 218.00 184.00 SPILLED 167.00 171.00 185.00 153.00 163.00 210.00 185.00 190.00 207.00 188.00 182.00 186.00 SPILLED SPILLED 213.00 187.00 228.00 189.00 213.00 197.00 221.00 217.00 199.00 189.00 177.00 189.00 199.00 214.00 187.00 175.00 210.00 176.00 193.00 153.00 162.00 211.00 179.00 193.00 215.00 186.00 186.00 174.00 SPILLED 241.00 211.00 183.00 215.00 174.00 220.00 200.00 220.00 207.00 195.00 189.00 175.00 134.00 196.00 214.00 SPILLED 165.00 206.00 170.00 189.00 153.00 158.00 201.00 179.00 192.00 205.00 185.00 182.00 182.00 SPILLED Covance 6329-183 3M T-6295 PAGE: 2 WEEK WEEK 910 SPILLED 212.00 188.00 213.00 184.00 218.00 195.00 218.00 211.00 200.00 185.00 170.00 128.00 198.00 205.00 SPILLED 165.00 209.00 170.00 191.00 160.00 157.00 SPILLED 174.00 SPILLED 203.00 189.00 184.00 186.00 185.00 225.0 0 202.0 0 177.0 0 201.0 0 175.0 0 209.0 0 188.0 0 216.0 0 206.0 0 194.0 0 181.0 0 166.0 0 124.0 0 190.0 0 202.0 0 181.0 0 167.0 0 207.0 0 166.0 0 189.0 0 148.0 0 158.0 0 204.0 0 177.0 0 SPILLEDi 210.0 0 183.0 0 180.0 0 185.0 0 191.0 0 1347 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 GROUP: MALE 1 - 0 PPM C92563 C92564 C92565 C92566 C92567 C92568 C92569 C92570 169.00 166.00 164.00 165.00 157.00 152.00 162.00 171.00 SPILLED 174.00 178.00 172.00 173.00 163.00 163.00 SPILLED GROUP: MALE 2 - 0 .5 PPM C92571 C92572 C92573 C92574 C92575 C92576 C92577 C92578 C92579 C92580 C92581 C92582 C92583 C92584 C92585 C92586 C92587 C92588 C92589 C92590 148.00 163.00 175.00 167.00 158.00 172.00 168.00 175.00 173.00 165.00 166.00 147.00 161.00 SPILLED 179.00 147.00 177.00 SPILLED 171.00 180.00 169.00 173.00 195.00 179.00 CNTMD 178.00 181.00 175.00 186.00 176.00 175.00 160.00 SPILLED 159.00 CNTMD 150.00 178.00 164.00 182.00 194.00 190.00 178.00 181.00 195.00 180.00 177.00 172.00 187.00 185.00 179.00 SPILLED SPILLED 159.00 185.00 194.00 175.00 199.00 183.00 188.00 SPILLED 169.00 166.00 186.00 167.00 187.00 173.00 197.00 204.00 200.00 186.00 184.00 203.00 192.00 182.00 182.00 194.00 197.00 195.00 193.00 206.00 194.00 185.00 192.00 199.00 183.00 198.00 195.00 NOT TAKEN NOT TAKEN 191.00 200.00 180.00 203.00 185.00 196.00 SPILLED SPILLED 167.00 168.00 164.00 187.00 176.00 198.00 212.00 182.00 197.00 224.00 194.00 196.00 185.00 208.00 192.00 190.00 157.00 SPILLED 166.00 197.00 185.00 213.00 193.00 206.00 208.00 205.00 199.00 198.00 192.00 195.00 189.00 195.00 196.00 196.00 198.00 233.00 191.00 SPILLED SPILLED 209.00 SPILLED 196.00 161.00 SPILLED 167.00 203.00 186.00 208.00 194.00 207.00 SPILLED 201.00 194.00 200.00 193.00 194.00 193.00 198.00 198.00 SPILLED 205.00 SPILLED 189.00 219.00 217.00 221.00 SPILLED 205.00 171.00 SPILLED 164.00 CNTMD 193.00 223.00 SPILLED 211.00 174.00 202.00 189.00 SPILLED 196.00 190.00 192.00 SPILLED 198.00 205.00 195.00 214.00 189.00 200.00 193.00 217.00 187.00 201.00 163.00 SPILLED 164.00 207.00 188.00 212.00 SPILLED 201.00 209.00 Covance 6329-183 3M T-6295 PAGE: 3 WEEK 9 WEEK 10 208.00 192.00 208.00 201.00 189.00 198.00 185.00 198.00 204.00 187.00 202.00 199.00 181.00 195.00 189.00 191.00 SPILLED 203.00 218.00 189.00 190.00 190.00 203.00 189.00 193.00 162.00 171.00 166.00 192.00 183.00 204.00 SPILLED 201.00 212.00 223.00 210.00 203.00 188.00 198.00 201.00 200.00 SPILLED 188.00 155.00 176.00 162.00 199.00 186.00 201.00 SPILLED 198.00 212.00 1348 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 GROUP: C92591 C92592 C92593 C92594 C92595 C92596 C92597 C92598 C92599 C92600 C92601 C92602 C92603 C92604 C92605 C92606 C92607 C92608 C92609 C92610 C92611 C92612 C92613 C92614 C92615 C92616 C92617 C92618 C92619 C92620 C92621 MALE 2 - 0..5 PPM 148.00 148.00 167.00 163.00 155.00 167.00 159.00 166.00 181.00 163.00 147.00 161.00 176.00 163.00 167.00 144.00 165.00 186.00 168.00 164.00 164.00 150.00 174.00 175.00 181.00 139.00 176.00 168.00 161.00 151.00 172.00 150.00 162.00 178.00 176.00 169.00 168.00 161.00 172.00 193.00 162.00 159.00 163.00 SPILLED 178.00 169.00 157.00 181.00 188.00 177.00 174.00 170.00 154.00 189.00 188.00 180.00 141.00 188.00 195.00 SPILLED 167.00 181.00 163.00 173.00 177.00 SPILLED 192.00 178.00 172.00 194.00 196.00 175.00 176.00 170.00 SPILLED 186.00 178.00 180.00 199.00 209.00 187.00 SPILLED 177.00 171.00 194.00 198.00 196.00 155.00 202.00 SPILLED 191.00 178.00 190.00 170.00 184.00 185.00 199.00 178.00 184.00 175.00 193.00 193.00 180.00 182.00 191.00 SPILLED 190.00 179.00 177.00 204.00 208.00 187.00 NOT TAKEN 182.00 172.00 NOT TAKEN SPILLED 168.00 SPILLED 209.00 NOT TAKEN 185.00 SPILLED 198.00 170.00 182.00 178.00 204.00 184.00 191.00 199.00 193.00 214.00 184.00 175.00 184.00 196.00 188.00 190.00 182.00 208.00 SPILLED 190.00 185.00 176.00 223.00 189.00 SPILLED 217.00 181.00 174.00 214.00 164.00 182.00 178.00 211.00 192.00 194.00 194.00 199.00 SPILLED 186.00 174.00 178.00 SPILLED 194.00 SPILLED 184.00 203.00 SPILLED 192.00 179.00 169.00 224.00 194.00 157.00 218.00 181.00 175.00 208.00 SPILLED 189.00 180.00 212.00 191.00 191.00 190.00 203.00 SPILLED 190.00 172.00 179.00 SPILLED 198.00 196.00 183.00 SPILLED SPILLED 194.00 177.00 175.00 232.00 197.00 163.00 221.00 191.00 176.00 209.00 SPILLED 186.00 168.00 207.00 187.00 190.00 186.00 196.00 215.00 182.00 170.00 SPILLED SPILLED 195.00 190.00 189.00 SPILLED SPILLED 185.00 173.00 181.00 SPILLED 194.00 167.00 213.00 179.00 179.00 216.00 Covance 6329-183 3M T-6295 PAGE: 4 WEEK 9 WEEK 10 163.00 182.00 171.00 SPILLED 180.00 190.00 SPILLED 192.00 218.00 181.00 166.00 183.00 196.00 195.00 194.00 193.00 207.00 214.00 180.00 169.00 175.00 SPILLED 188.00 SPILLED 210.00 179.00 170.00 211.00 166.00 186.00 172.00 204.00 182.00 192.00 187.00 190.00 211.00 177.00 160.00 175.00 195.00 192.00 190.00 193.00 206.00 222.00 178.00 170.00 183.00 SPILLED 181.00 170.00 199.00 175.00 150.00 203.00 1349 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 GROUP: MALE 2 - 0 .5 PPM C92622 C92623 C92624 C92625 C92626 C92627 C92628 C92629 C92630 CNTMD 143.00 CNTMD 163.00 175.00 177.00 168.00 164.00 187.00 166.00 153.00 150.00 SPILLED 176.00 188.00 180.00 178.00 188.00 GROUP: MALE 3 - 2 PPM C92631 C92632 C92633 C92634 C92635 C92636 C92637 C92638 C92639 C92640 C92641 C92642 C92643 C92644 C92645 C92646 C92647 C92648 C92649 166.00 192.00 154.00 149.00 192.00 200.00 137.00 173.00 151.00 159.00 154.00 168.00 156.00 171.00 169.00 197.00 151.00 155.00 140.00 174.00 193.00 160.00 162.00 205.00 SPILLED 145.00 182.00 156.00 171.00 175.00 176.00 160.00 171.00 169.00 194.00 168.00 165.00 153.00 175.00 157.00 166.00 SPILLED 183.00 203.00 190.00 191.00 197.00 176.00 200.00 SPILLED 172.00 209.00 197.00 152.00 200.00 163.00 169.00 176.00 187.00 168.00 173.00 SPILLED 190.00 173.00 171.00 159.00 187.00 169.00 167.00 SPILLED 193.00 SPILLED 219.00 216.00 197.00 185.00 171.00 172.00 SPILLED 190.00 234.00 SPILLED 214.00 202.00 180.00 187.00 148.00 SPILLED SPILLED SPILLED 161.00 211.00 166.00 170.00 192.00 196.00 170.00 174.00 185.00 NOT TAKEN 171.00 169.00 165.00 180.00 211.00 170.00 181.00 205.00 SPILLED 154.00 218.00 176.00 164.00 187.00 196.00 165.00 169.00 185.00 172.00 164.00 167.00 189.00 174.00 169.00 SPILLED 187.00 SPILLED 220.00 SPILLED 204.00 180.00 209.00 178.00 179.00 213.00 204.00 156.00 223.00 185.00 165.00 189.00 200.00 164.00 170.00 190.00 173.00 166.00 173.00 188.00 174.00 177.00 SPILLED 201.00 SPILLED 215.00 213.00 229.00 180.00 209.00 181.00 178.00 SPILLED 212.00 153.00 227.00 SPILLED 172.00 183.00 188.00 162.00 158.00 182.00 171.00 157.00 173.00 187.00 153.00 176.00 SPILLED 205.00 SPILLED 215.00 215.00 242.00 187.00 214.00 181.00 179.00 201.00 227.00 159.00 228.00 186.00 176.00 188.00 184.00 165.00 163.00 185.00 172.00 161.00 166.00 Covance 6329-183 3M T-6295 PAGE: 5 WEEK 9 WEEK 10 183.00 155.00 178.00 213.00 202.00 SPILLED SPILLED 212.00 222.00 176.00 164.00 177.00 SPILLED 202.00 SPILLED SPILLED SPILLED 225.00 172.00 202.00 179.00 171.00 197.00 SPILLED 146.00 223.00 171.00 164.00 185.00 180.00 165.00 163.00 184.00 167.00 154.00 162.00 176.00 208.00 179.00 182.00 SPILLED SPILLED 152.00 226.00 176.00 172.00 192.00 183.00 168.00 168.00 196.00 171.00 166.00 173.00 1350 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 GROUP: MALE 3 - 2 PPM C92650 C92651 C92652 C92653 C92654 C92655 C92656 C92657 C92658 C92659 C92660 C92661 C92662 C92663 C92664 C92665 C92666 C92667 C92668 C92669 C92670 C92671 C92672 C92673 C92674 C92675 C92676 C92677 C92678 C92679 C92680 160.00 176.00 159.00 158.00 170.00 167.00 152.00 164.00 161.00 182.00 173.00 164.00 149.00 180.00 165.00 183.00 176.00 167.00 162.00 154.00 163.00 154.00 144.00 176.00 174.00 178.00 175.00 149.00 143.00 168.00 169.00 176.00 189.00 173.00 168.00 187.00 184.00 160.00 188.00 170.00 193.00 167.00 168.00 163.00 201.00 185.00 190.00 184.00 185.00 172.00 165.00 172.00 165.00 149.00 170.00 174.00 183.00 172.00 157.00 158.00 173.00 192.00 180.00 194.00 183.00 175.00 202.00 201.00 169.00 183.00 187.00 195.00 194.00 174.00 187.00 215.00 187.00 201.00 198.00 205.00 183.00 178.00 177.00 183.00 157.00 175.00 170.00 188.00 SPILLED 160.00 166.00 177.00 199.00 NOT TAKEN 194.00 NOT TAKEN 182.00 203.00 208.00 184.00 176.00 216.00 207.00 221.00 175.00 SPILLED 230.00 SPILLED 207.00 203.00 209.00 NOT TAKEN 184.00 177.00 186.00 160.00 175.00 182.00 184.00 154.00 160.00 NOT TAKEN 180.00 195.00 197.00 179.00 SPILLED 221.00 187.00 SPILLED 189.00 203.00 178.00 172.00 176.00 SPILLED 197.00 207.00 206.00 210.00 184.00 182.00 186.00 153.00 185.00 171.00 183.00 188.00 164.00 204.00 207.00 187.00 177.00 SPILLED 223.00 183.00 SPILLED SPILLED 206.00 197.00 172.00 173.00 SPILLED 197.00 208.00 203.00 213.00 188.00 181.00 191.00 170.00 182.00 SPILLED 183.00 176.00 SPILLED 187.00 SPILLED 190.00 178.00 SPILLED SPILLED 189.00 197.00 194.00 211.00 180.00 170.00 170.00 SPILLED 207.00 207.00 207.00 218.00 190.00 177.00 152.00 177.00 184.00 SPILLED 183.00 178.00 152.00 186.00 194.00 192.00 184.00 203.00 SPILLED 186.00 SPILLED 198.00 205.00 190.00 177.00 175.00 SPILLED 196.00 213.00 206.00 221.00 197.00 177.00 188.00 180.00 180.00 SPILLED 179.00 178.00 165.00 195.00 SPILLED Covance 6329-183 3M T-6295 PAGE: 6 WEEK WEEK 910 193.00 188.00 190.00 SPILLED 181.00 189.00 184.00 203.00 176.00 157.00 168.00 203.00 191.00 211.00 204.00 209.00 194.00 165.00 183.00 173.00 179.00 204.00 184.00 168.00 169.00 193.00 208.00 192.00 183.00 SPILLED SPILLED 184.00 196.00 199.00 211.00 SPILLED 167.00 175.00 SPILLED 192.00 210.00 209.00 213.00 197.00 177.00 194.00 185.00 SPILLED 216.00 186.00 169.00 172.00 196.00 215.00 1351 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 GROUP: MALE 3 - 2 PPM C92681 C92682 C92683 C92684 C92685 C92686 C92687 C92688 C92689 C92690 152.00 150.00 170.00 163.00 151.00 161.00 170.00 155.00 155.00 194.00 SPILLED 165.00 171.00 160.00 155.00 165.00 167.00 157.00 164.00 210.00 GROUP: MALE 4 - 5 PPM C92691 C92692 C92693 C92694 C92695 C92696 C92697 C92698 C92699 C92700 C92701 C92702 C92703 C92704 C92705 C92706 C92707 C92708 161.00 148.00 175.00 163.00 182.00 166.00 167.00 180.00 148.00 151.00 154.00 161.00 153.00 157.00 151.00 165.00 149.00 172.00 164.00 151.00 SPILLED 156.00 181.00 170.00 175.00 175.00 149.00 157.00 161.00 166.00 170.00 165.00 154.00 174.00 162.00 178.00 167.00 173.00 185.00 167.00 172.00 176.00 177.00 168.00 173.00 SPILLED 174.00 173.00 187.00 163.00 184.00 178.00 187.00 189.00 157.00 155.00 173.00 172.00 187.00 175.00 169.00 174.00 164.00 184.00 SPILLED 164.00 182.00 162.00 179.00 162.00 188.00 178.00 174.00 228.00 172.00 167.00 194.00 163.00 SPILLED SPILLED 182.00 189.00 157.00 156.00 164.00 173.00 192.00 180.00 176.00 173.00 157.00 172.00 175.00 174.00 189.00 180.00 186.00 180.00 202.00 SPILLED 180.00 217.00 177.00 173.00 202.00 SPILLED 190.00 SPILLED SPILLED 197.00 158.00 149.00 171.00 178.00 194.00 185.00 184.00 174.00 157.00 158.00 SPILLED 174.00 192.00 177.00 182.00 183.00 202.00 SPILLED 187.00 206.00 189.00 186.00 202.00 176.00 SPILLED SPILLED 197.00 191.00 166.00 150.00 169.00 171.00 203.00 186.00 178.00 176.00 152.00 162.00 SPILLED 172.00 186.00 174.00 191.00 185.00 201.00 SPILLED 182.00 133.00 193.00 183.00 198.00 182.00 194.00 200.00 196.00 SPILLED 149.00 148.00 174.00 176.00 204.00 190.00 191.00 172.00 155.00 171.00 SPILLED 180.00 SPILLED 180.00 196.00 182.00 205.00 SPILLED 173.00 230.00 181.00 182.00 191.00 183.00 SPILLED SPILLED SPILLED 195.00 158.00 157.00 171.00 171.00 205.00 191.00 189.00 174.00 157.00 174.00 Covance 6329-183 3M T-6295 PAGE: 7 WEEK WEEK 910 SPILLED 170.00 180.00 172.00 190.00 177.00 199.00 SPILLED 158.00 218.00 SPILLED 180.0 0 187.0 0 175.0 0 198.0 0 180.0 0 202.0 0 SPILLEDi 164.0 0 220.0 0 185.00 179.00 192.00 185.00 172.00 SPILLED 213.00 194.00 160.00 154.00 177.00 176.00 205.00 194.00 195.00 179.00 165.00 179.00 181.0 0 177.0 0 193.0 0 181.0 0 177.0 0 SPILLEDi 196.0 0 191.0 0 168.0 0 150.0 0 162.0 0 168.0 0 205.0 0 184.0 0 195.0 0 171.0 0 151.0 0 173.0 0 1352 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 GROUP: C92709 C92710 C92711 C92712 C92713 C92714 C92715 C92716 C92717 C92718 C92719 C92720 C92721 C92722 C92723 C92724 C92725 C92726 C92727 C92728 C92729 C92730 C92731 C92732 C92733 C92734 C92735 C92736 C92737 C92738 C92739 MALE 4 - 5 PPM 176.00 158.00 153.00 182.00 199.00 175.00 168.00 179.00 171.00 164.00 151.00 200.00 161.00 174.00 172.00 201.00 158.00 158.00 151.00 163.00 170.00 158.00 SPILLED 159.00 159.00 170.00 173.00 157.00 151.00 140.00 144.00 180.00 170.00 155.00 188.00 SPILLED SPILLED SPILLED 186.00 177.00 176.00 162.00 SPILLED 176.00 179.00 197.00 215.00 170.00 173.00 166.00 173.00 191.00 170.00 SPILLED 177.00 166.00 179.00 180.00 164.00 160.00 157.00 148.00 175.00 176.00 168.00 189.00 SPILLED 203.00 184.00 197.00 187.00 191.00 169.00 SPILLED 182.00 187.00 SPILLED 216.00 173.00 186.00 178.00 201.00 214.00 186.00 173.00 195.00 175.00 184.00 180.00 176.00 174.00 180.00 159.00 165.00 180.00 161.00 186.00 SPILLED 184.00 NOT TAKEN 179.00 179.00 NOT TAKEN 169.00 SPILLED 147.00 186.00 SPILLED 216.00 154.00 183.00 SPILLED 201.00 210.00 SPILLED NOT TAKEN 174.00 165.00 NOT TAKEN 182.00 170.00 167.00 170.00 160.00 187.00 SPILLED 173.00 200.00 182.00 193.00 203.00 179.00 173.00 SPILLED 156.00 196.00 SPILLED 222.00 179.00 191.00 187.00 207.00 217.00 186.00 196.00 168.00 189.00 177.00 176.00 182.00 167.00 187.00 192.00 172.00 204.00 SPILLED 192.00 207.00 186.00 176.00 SPILLED 171.00 189.00 SPILLED 230.00 172.00 188.00 190.00 215.00 209.00 188.00 189.00 163.00 189.00 177.00 172.00 181.00 164.00 195.00 SPILLED 181.00 SPILLED SPILLED SPILLED 211.00 190.00 179.00 SPILLED 173.00 192.00 SPILLED SPILLED 168.00 192.00 190.00 215.00 209.00 189.00 195.00 162.00 192.00 176.00 172.00 188.00 167.00 185.00 SPILLED 174.00 197.00 SPILLED SPILLED 205.00 186.00 177.00 SPILLED 173.00 192.00 207.00 217.00 166.00 192.00 SPILLED 208.00 206.00 178.00 191.00 167.00 186.00 175.00 171.00 181.00 167.00 Covance 6329-183 3M T-6295 PAGE: 8 WEEK 9 WEEK 10 179.00 SPILLED 174.00 SPILLED SPILLED 210.00 203.00 192.00 SPILLED SPILLED 178.00 200.00 SPILLED 223.00 SPILLED 187.00 187.00 202.00 213.00 179.00 198.00 180.00 190.00 186.00 184.00 191.00 184.00 170.00 192.00 168.00 201.00 199.00 206.00 198.00 179.00 181.00 SPILLED 161.00 145.00 SPILLED SPILLED 172.00 183.00 185.00 215.00 205.00 182.00 196.00 177.00 184.00 179.00 177.00 190.00 177.00 1353 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 GROUP: MALE 4 - 5 PPM C92740 C92741 C92742 C92743 C92744 C92745 C92746 C92747 C92748 C92749 C92750 169.00 156.00 176.00 164.00 152.00 170.00 182.00 158.00 160.00 149.00 167.00 178.00 163.00 190.00 183.00 178.00 183.00 201.00 163.00 165.00 152.00 180.00 GROUP: MALE 5 - 2 0 PPM C92751 C92752 C92753 C92754 C92755 C92756 C92757 C92758 C92759 C92760 C92761 C92762 C92763 C92764 C92765 C92766 C92767 150.00 145.00 184.00 141.00 148.00 167.00 149.00 138.00 154.00 166.00 155.00 160.00 171.00 190.00 156.00 162.00 154.00 172.00 146.00 194.00 154.00 154.00 187.00 154.00 153.00 161.00 180.00 176.00 165.00 178.00 207.00 170.00 172.00 177.00 185.00 167.00 203.00 191.00 193.00 197.00 202.00 175.00 177.00 160.00 198.00 183.00 176.00 199.00 198.00 NOT TAKEN 200.00 185.00 179.00 181.00 160.00 190.00 184.00 177.00 205.00 206.00 207.00 206.00 189.00 192.00 167.00 193.00 185.00 180.00 205.00 204.00 210.00 SPILLED 196.00 201.00 176.00 188.00 199.00 198.00 204.00 208.00 210.00 SPILLED 195.00 228.00 177.00 SPILLED 196.00 SPILLED 208.00 SPILLED 205.00 SPILLED 197.00 SPILLED 172.00 191.00 189.00 158.00 197.00 167.00 174.00 213.00 170.00 163.00 173.00 191.00 174.00 178.00 195.00 218.00 181.00 174.00 197.00 189.00 NOT TAKEN 204.00 164.00 164.00 217.00 179.00 161.00 NOT TAKEN 189.00 180.00 184.00 189.00 201.00 183.00 172.00 195.00 191.00 210.00 166.00 171.00 217.00 180.00 165.00 189.00 176.00 185.00 199.00 220.00 189.00 178.00 188.00 186.00 192.00 164.00 164.00 226.00 182.00 171.00 187.00 178.00 189.00 193.00 220.00 188.00 175.00 204.00 SPILLED 206.00 177.00 170.00 SPILLED SPILLED 169.00 196.00 172.00 183.00 205.00 218.00 198.00 176.00 206.00 SPILLED 201.00 159.00 164.00 SPILLED SPILLED 163.00 185.00 176.00 SPILLED 202.00 222.00 187.00 174.00 221.00 Covance 6329-183 3M T-6295 PAGE: 9 WEEK WEEK 910 196.00 178.00 208.00 210.00 205.00 198.00 SPILLED 225.00 178.00 179.00 181.00 176.00 204.00 208.00 198.00 190.00 SPILLED SPILLED SPILLED 178.00 182.00 205.00 159.00 176.00 SPILLED 175.00 160.00 183.00 170.00 173.00 203.00 SPILLED 187.00 177.00 225.00 182.00 173.00 163.00 172.00 SPILLED 172.00 157.00 186.00 171.00 177.00 200.00 207.00 187.00 176.00 224.00 1354 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 GROUP: C92768 C92769 C92770 C92771 C92772 C92773 C92774 C92775 C92776 C92777 C92778 C92779 C92780 C92781 C92782 C92783 C92784 C92785 C92786 C92787 C92788 C92789 C92790 C92791 C92792 C92793 C92794 C92795 C92796 C92797 C92798 MALE 5 - 2 0 PPM 167.00 169.00 177.00 162.00 167.00 147.00 160.00 170.00 170.00 153.00 157.00 147.00 146.00 160.00 151.00 172.00 161.00 154.00 151.00 174.00 183.00 143.00 161.00 150.00 156.00 163.00 151.00 148.00 137.00 149.00 161.00 187.00 177.00 SPILLED 167.00 174.00 160.00 171.00 186.00 181.00 161.00 182.00 155.00 166.00 170.00 160.00 178.00 164.00 167.00 156.00 181.00 204.00 151.00 164.00 153.00 183.00 160.00 153.00 157.00 142.00 154.00 158.00 197.00 200.00 190.00 194.00 194.00 167.00 173.00 202.00 185.00 165.00 192.00 163.00 155.00 172.00 179.00 194.00 187.00 180.00 169.00 199.00 213.00 169.00 177.00 174.00 179.00 164.00 169.00 165.00 149.00 161.00 170.00 195.00 186.00 181.00 207.00 177.00 167.00 175.00 197.00 185.00 164.00 190.00 NOT TAKEN 160.00 172.00 178.00 190.00 167.00 180.00 165.00 201.00 SPILLED 168.00 175.00 175.00 176.00 NOT TAKEN 156.00 165.00 154.00 162.00 NOT TAKEN 203.00 191.00 191.00 SPILLED 182.00 168.00 189.00 208.00 189.00 168.00 180.00 164.00 172.00 184.00 198.00 193.00 185.00 173.00 196.00 219.00 171.00 179.00 170.00 188.00 162.00 167.00 158.00 162.00 208.00 189.00 182.00 188.00 184.00 157.00 192.00 204.00 188.00 162.00 183.00 165.00 175.00 180.00 200.00 189.00 190.00 172.00 194.00 197.00 172.00 181.00 174.00 186.00 166.00 168.00 162.00 164.00 200.00 197.00 193.00 SPILLED 175.00 159.00 194.00 211.00 187.00 173.00 189.00 172.00 176.00 190.00 201.00 175.00 189.00 180.00 202.00 200.00 177.00 185.00 181.00 190.00 170.00 175.00 155.00 166.00 180.00 189.00 185.00 175.00 174.00 155.00 189.00 207.00 186.00 163.00 185.00 171.00 138.00 170.00 192.00 179.00 SPILLED 178.00 138.00 167.00 163.00 177.00 175.00 187.00 167.00 180.00 153.00 166.00 Covance 6329-183 3M T-6295 PAGE: 10 WEEK 9 WEEK 10 188.00 188.00 183.00 176.00 182.00 163.00 196.00 195.00 185.00 166.00 183.00 172.00 168.00 177.00 181.00 178.00 181.00 166.00 SPILLED 198.00 168.00 175.00 164.00 181.00 163.00 178.00 148.00 163.00 181.00 188.00 184.00 172.00 176.00 150.00 185.00 202.00 186.00 169.00 186.00 SPILLED 168.00 176.00 186.00 181.00 183.00 175.00 SPILLED 194.00 SPILLED 176.00 170.00 182.00 161.00 177.00 148.00 167.00 1355 164.00 174.00 203.00 SPILLED 189.00 Covance 6329-183 3M T-6295 167.00 171.00 192.00 216.00 181.00 170.00 168.00 179.00 222.00 SPILLED 176.00 171.00 188.00 222.00 SPILLED 181.00 175.00 185.00 226.00 171.00 i i ft i ft o rH i ft rH rH ii & i ft CD Cft i i i i i i ft i ft cn i ft ii & i i rti Q 3CQ T5 O dft) ft C U T5 O fOt rti g T5 Eh fEfhiCft 1 ft 1 H co i Wi & ii i 1>h Q -- 1 LD 1 CF> 1 (N l LD l >h i Eh Eh l ft l W l> l H1 1& 1 H CD O ftlft CD f t O~ h Eh U ft ft < <w U 2 Q fPt 1 % CD CD 1 l 1X EMh U <ft <f)t 11 11 M XQ Om Eh U C U1 HU 1 H1 O 1 ft O i ft ft i U ft i ftf t PU ) 1i C ft Eh ft H SEhJ QU O ft O ftlft ft O &ifPt *sFft o ft H ftl ft ft H *X) H & 1 1 i i ii i 11 ft 1 W LD 1 W1 & 111 ft ft ^ ft i i i i i ii i i 1 ft 1 ftro i ft i ii ft ffttlCN & O OOOOOOOOOOOOOOOOOOO OnOOOOOOOOOOOOOOOOOOOQ CO wpftLCDO oi [> [> ro [> l> CO rH ID CO CO LD cn [> LD [> cn cn (N [> ro CO l> ro CO rH LD O O rH rH [> Hfftt ft ftrH H rH rH rH rH rH rH rH rH rH rH rH rH rH rH rH rH (N rH rH M w CD O OOOOOOOOOOOoOOOOOOo OnnOOOOOOOOOOOoOOOOOOo O wftwftCO rH LD l> cn rH CO l> ro (N O rH (N l> ro rH CO f t ft CO [> CO rH [> LD CO [> cn CO ro CO cn LD CO ftrH H H rH rH rH rH rH rH rH rH rH rH rH rH rH rH rH rH rH rH rH ft ww oOOOOOOOOOOOOOOOOOOOOO oOOOOOOOOOOOOOOOOOOOOO CO rH (N ro ro ro l> CO rH LD CO O CO l> [> 1> [> cn [> CO [> CO CO LD CO CO cn O cn CO LD rH [> LD [> rH rH rH rH rH rH rH rH rH rH rH rH rH (N rH rH rH rH (N rH rH rH o O O O O O O O O O O O oO O O O O O O O onO O O O O O O O O O O O O O O O O O O O ro CO wffttrcon CO [> CO cn LD cn cn [> CO LD CO (N CO (N O (N CO CO cn LD cn O LD LD cn LD rH CO rH H rH rH rH rH rH rH rH rH rH rH (N rH rH rH rH rH rH rH rH rH oOOOOOOOOOOOOOOOOOOOOO oOOOOOOOOOOOOOOOOOOOOO o cn LD LD rH l> rH CO l> O O rH rH rH rH CO rH LD cn [> cn [> CO [> CO [> CO LD [> CO O CO CO LD ro LD cn CO rH rH rH rH rH rH rH rH rH rH rH rH (N rH rH rH rH rH rH rH rH rH oOooOOOOOOOOOOOOOOOOOO OOooOOOOOOOOOOOOOOOOOO 1 1ro (N ro O LD ro rH L-" O CO (N U) ro (N ro LD cn L-" rH CO CO o [> cn CO cn CO cn cn CO O > CO LD > LD cn LD CO rH rH (N rH rH rH rH rH rH rH rH rH (N rH rH rH rH rH rH rH rH rH o oOOOOOOOOOOOOO oOOOO on oOOOOOOOOOOOOOn oOOOO ro wffttLc-n" L-" LD LD CO ftL-" CO cn O ro (N LD w ftLD [> LD LD 1> CO [> LD CO LD cn CO CO O LD [> rH H rH rH rH rH rH rH rH rH rH rH rH rH rH rH H rH rH rH rH rH f t ft U) U) oOOOOOOOOOOOOOOOOOOOOO oOOOOOOOOOOOOOOOOOOOOO 1cn O cn L-"rH CO ro cn LD O LD ro (N LD L-"L-"O ro ro CO cn [> LD CO [> [> > CO cn CO LD CO rH rH rH rH rH rH rH rH rH rH rH rH rH rH rH rH rH rH rH rH rH rH ^roroHHrococorooriMnoooiaih'fiDMh Lni>i>'X'i>Ln'X''X''X'Lni>i>i>'X'coLn^roi>LnLn'X' r lr lr lr lr lr lr lr lr lr lr lr lr lH H H H r lr lr lr lr l O O rH O O O rH O O LD rH O O O [> rH O O (N [> rH O O [> LD rH O O cn LD rH o o (N LD rH 174.00 184.00 181.00 224.00 172.00 167.00 179.00 176.00 211.00 171.00 164.00 186.00 169.00 206.00 174.00 160.00 173.00 164.00 195.00 SPILLED 157.00 169.00 167.00 185.00 157.00 ft 11 ftl H ft l111111 ft ft C ft 2i--i f t P < 1 1 1 1 1 1 ii i i , ooooooooooooooooooooo ooooooooooooooooooooon LD ftCO \o \o U ) (N cn CO U ) rH U ) ro \o \o cn cn U) rH w ft ftLD \o \o LD LD 1> LD LD 1> LD LD \> LD (N LD LD LD ft rH rH rH rH rH rH rH rH rH rH rH rH rH rH rH rH rH rH rH rH rH M \o H ft 2< f t < ! 2 U1 o o H rH f t ft PP ftO ft cn O rH (N ro LD \o [> CO cn O rH (N ro LD \o [> CO cn o O H o cn O O o o o o o o o O rH rH rH rH rH rH rH rH rH rH (N O (N [> CO CO CO CO CO CO CO CO CO CO CO CO CO CO CO CO CO CO CO CO CO CO (N (N (N (N (N (N (N (N (N (N (N (N (N (N (N (N (N (N (N (N(N(N (N cn cn cn cn cn cn cn cn cn cn cn cn cn cn cn cn cn cn cn cn cn cn cn Uu u u u u u u u u u UUUUUUUUUUu U C92822 C92823 C92824 C92825 C92826 Covance 6329-183 3M T-6295 ft ftO HH P W O Cft 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 Ui 1 Hi Ci ft i i ffftttft 3 ftft CO ft 3 IN -.i ID 1 ft 1 Ol 1 L> 1 XI Eh Eh ft W i> H 3 coHft'X'i>ro(NftHLn'X'oi>Lncoi>coi>Hco'X'ooco'X'roi>ftLno(N C O ftO ftftL D C O I> ' > rH C O C O rO L D L D C O ft^ ftftrH [> rH C O I> ft' > C O C O I> C O H r l0 1 H H r lH r lr l0 ) H r lf'] r lr lr lr lr lr lr lf'lH C '] H r lH r lH r lH r l o OOo O OOOOOOo OOOO OOo O OO OO o OOo On n OOOOOOo OOOOn OOo n n On OOn OO fto w (N ro CO CN CO o CO f t f t rH rH (N rH rH M ft ftw rH ro L> ro LD CO l> l> f t CO L> [> CO CO ro LD LD CO f t M rH rH rH rH rH (N rH rH rH rH ft w ft(N LD ft ft rH ft O LD LD ftrH M rH (N rH ftw w ft f t f t [> M M rH ft ft wft f t [> M rH ft rH wftftO CO f t LD CO rH H rH rH ft ui ui UI UI UI UI UI o Oo o O OOOOO OOOOOo OOO o Oo OO OO o Oo o On n OOOOOn OOOOOo OOOn o Oo OOnOO rH rH LD L> rH w ft w ft LD ft CO [> ro w ft rH O ft (N rH LD ro w ft ft rH LD w ft O LD ft ft o CO O ft ft [> LD rH CO CO ft LD CO ft LD CO ft rH ft CO [> ft CO ft ft [> ft rH rH (N rH (N H H rH rH rH rH rH H rH rH rH rH rH rH rH CN rH M rH rH rH rH rH M rH rH ft ft ft ft ft ui ui UI UI UI OOoOO OOOOOoOOOOOOOoOoOOOOOoOO OOoOOnnOOOOOoOOOOOOOOOoOOOOOoOO ft ft ftft rH ft LD w ft w ft ft ft CO ft o L> ft ft ft [> LD CO ro L> ft LD l> l> l> ft (N CN ft O LD ro CO ro LD LD ft ft LD CO O o CO CO CO [> CO O O (N [> ft ftftrH rH CN rH rH M M rH rH rH rH rH (N rH rH rH rH rH rH rH (N rH (N rH rH rH rH rH rH rH rH ui w 1357 rd Q TtJ dft) dCtO o u ft C o fot rti T5 d ftft i i O' i 5i Hf t i i f t < < w u 2 Q fPt 1 % UI UI X< EMh ft <) U ft M 1 1 1 1 11 1 XQ Oh Eh U C U Mu 1 fftto5 M 5 <f t) 1 1 1 1 u ft 1 >ftH wp C ft Eh - ftfMt H U 1 1 O1 XO 1 W Oi 1 WO 1 &IfPt *F ft o ft H ft ft X ft LD ft p i i 1 1 11 X W LD W 3 1 1 i 1 1 11 1 X H^ W P 1 1 i i ii X H ro W P 1 i i i ii X W CN W P X WH H P OOOOOOOOOOOOOoOOOOOOOOO OOOO OO OOOOOOOOOOOOOoOOOOOOOOOnOOOOnOO ft ft ftftrH CO ro O LD LD O l> rH CO O CO [> CO ro (N o CO ww ft ft ftCO O ro O Ol l> Ol l> ro Ol ro ftft ftft ftCO O LD ft ft O [> [> [> [> rH rH (N rH rH rH rH rH rH rH rH rH rH rH rH O l rH rH rH O l rH rH H rH rH rH rH H rH rH o OOOOOOOOOOoOoOOOo OOOOOOOOOOOOO o OOOOOOOOOOoOoOOOo OOOOOOOOOOOOO [> O l LD rH O l O ro rH ft ft ro l> ro o rH ro ro O l l> ft O CO O rH O rH ro O CO ft O ft ft [> CO CO LD ft o CO LD f t O LD ft CO O ft CO CO CO [> O CO [> ft rH rH O l rH rH rH rH rH rH rH O l rH O l rH rH rH O l rH rH rH O l rH rH rH rH rH rH O l rH rH rH o OOOOOOOOOOOOOOOo o OOOOOOOOO OO o OOOOOOOOOOOOOOOo o OOOOOOOOOn n OO ftCO rH f t rH O O l CO LD rH LD LD ro LD ft LD O l O O O ft ft w L-" f t w ft rH LD [> CO CO CO f t CO CO LD f t O CO [> o o LD CO CO rH f t CO [> f t [> f t f t [> f t rH rH rH rH rH rH rH rH rH rH O l rH ftftrH rH O l O l rH rH rH O l rH rH rH rH rH rH H H rH rH ui w o OOOOo OOOOOO OOOo OOOOOOOOOOo OO o OOOOo OOo OOOn OOOo OOOOOOOOOOo n OO LD L-" LD O l O l ro ftw rH CO ro Ol ro L-" w LD rH L-" rH ro ro O l f t CO [> CO CO [> O CO CO LD f t f t CO f t LD o rH LD CO [> rH LD f t [> [> CO [> rH f t [> CO rH rH rH rH O l rH rH rH rH rH rH rH H rH rH O l O l rH rH rH O l rH rH rH rH rH rH O l M rH rH o OOOOOOOOO OOOo OOOO o OO OOOOOO OO o OOOOOOOOO OOOo OOOOn o OO OOOOOOn OO rH rH O O ft L> CO LD ro O l ro CO LD O l L-" O [> [> [> ft LD LD CO CO [> rH LD ft O CO w ft rH CO ft [> ft L> O l L-" CO [> O [> Ol O w ft ft rH CO ft ft2 rH rH rH rH rH rH rH rH rH rH rH rH O l rH rH rH O l rH H rH rH rH rH rH rH rH rH M rH rH ft ft Ul Ul o 1 OOOOOOOOOOOOOOOOoO OOO OOOOOOo OO OOOOOOOOOO OOOOOOo On OOO OOOOOOo OO ft ftft ft ft ftH L-" L-" LD L-" CO O l L-" LD 1> CO LD LD CO rH O [> [> O CO LD ro o LD O w ft O LD ro L> LD [> [> [> ro O l LD LD L-" O CO 1> [> rH rH rH rH rH rH rH rH rH rH rH rH O l rH rH rH rH rH H rH rH rH rH rH rH rH rH rH rH rH rH C ft 2 Ul f t ft CW 2i-- i Sf t SP <S 1 1 ii i 1 ft p ft ft ft ft1> CO o rH ro LD 1> CO o rH ro LD 1> CO o rH ro LD LD 1> o O l O l ro ro ro ro ro ro ro ro ro ro LD LD LD LD LD LD LD LD CO CO CO CO CO CO CO CO CO CO CO CO CO CO CO CO CO CO CO CO CO CO CO CO CO CO CO CO CO CO CO ftftftftft ftft ft ftftftftO l Ol Ol Ol Ol Ol Ol Ol Ol Ol Ol Ol Ol Ol Ol Ol Ol ft ft ft ft ft ft ft ft ft ft ft ft ft ft ft ft ft ft ft UuuuUUUUUUUUUuuuuuuuuuuuuuuuuUU Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 GROUP: C92858 C92859 C92860 GROUP: C92861 C92862 C92863 C92864 C92865 C92866 C92867 C92868 C92869 C92870 C92871 C92872 C92873 C92874 C92875 C92876 C92877 C92878 C92879 C92880 C92881 C92882 C92883 C92884 C92885 MALE 6 - 20 PPM 148.00 160.00 160.00 152.00 173.00 167.00 FEMALE 1 - 0 PPM 140.00 111.00 122.00 SPILLED 151.00 124.00 130.00 126.00 123.00 125.00 123.00 110.00 116.00 136.00 114.00 109.00 117.00 SPILLED 130.00 CNTMD SPILLED 118.00 140.00 137.00 140.00 151.00 121.00 136.00 160.00 146.00 136.00 145.00 152.00 SPILLED 143.00 138.00 115.00 125.00 141.00 127.00 121.00 132.00 151.00 144.00 124.00 143.00 129.00 157.00 144.00 151.00 186.00 188.00 184.00 187.00 186.00 183.00 198.00 198.00 178.00 155.00 128.00 127.00 SPILLED 155.00 135.00 150.00 157.00 SPILLED 146.00 TECH SPILL 113.00 141.00 162.00 123.00 130.00 140.00 SPILLED 150.00 123.00 151.00 126.00 173.00 147.00 160.00 NOT TAKEN 120.00 128.00 NOT TAKEN 149.00 123.00 145.00 154.00 SPILLED 124.00 140.00 116.00 125.00 SPILLED 130.00 SPILLED SPILLED SPILLED 135.00 122.00 129.00 SPILLED SPILLED 133.00 154.00 133.00 132.00 149.00 126.00 156.00 167.00 SPILLED 136.00 140.00 119.00 125.00 144.00 124.00 126.00 SPILLED SPILLED 145.00 135.00 146.00 128.00 173.00 SPILLED 163.00 187.00 199.00 181.00 117.00 120.00 143.00 124.00 129.00 SPILLED SPILLED 126.00 137.00 107.00 118.00 SPILLED SPILLED 118.00 SPILLED SPILLED 146.00 125.00 139.00 115.00 SPILLED 125.00 149.00 194.00 202.00 178.00 116.00 131.00 144.00 122.00 141.00 130.00 SPILLED 148.00 131.00 105.00 129.00 135.00 SPILLED 120.00 SPILLED SPILLED 147.00 131.00 149.00 110.00 SPILLED 141.00 145.00 190.00 191.00 174.00 120.00 135.00 136.00 125.00 138.00 147.00 SPILLED 137.00 141.00 96.00 125.00 SPILLED SPILLED 117.00 SPILLED SPILLED 152.00 147.00 142.00 114.00 156.00 SPILLED 146.00 Covance 6329-183 3M T-6295 PAGE: 13 WEEK 9 WEEK 10 188.00 SPILLED 182.00 189.00 SPILLED 176.00 119.00 129.00 148.00 129.00 132.00 147.00 SPILLED 127.00 130.00 99.00 SPILLED 119.00 128.00 115.00 SPILLED SPILLED 154.00 131.00 145.00 117.00 161.00 142.00 141.00 126.00 SPILLED 146.00 129.00 142.00 151.00 SPILLED 139.00 132.00 106.00 136.00 124.00 130.00 120.00 SPILLED SPILLED 159.00 140.00 156.00 115.00 167.00 146.00 153.00 1358 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 GROUP: C92886 C92887 C92888 C92889 C92890 C92891 C92892 C92893 C92894 C92895 C92896 C92897 C92898 C92899 C92900 C92901 C92902 C92903 C92904 C92905 C92906 C92907 C92908 C92909 C92910 C92911 C92912 C92913 C92914 C92915 C92916 FEMALE 1 - 0 PPM 142.00 133.00 142.00 117.00 SPILLED SPILLED 130.00 127.00 118.00 132.00 128.00 122.00 129.00 137.00 SPILLED 109.00 155.00 152.00 119.00 113.00 SPILLED SPILLED 123.00 117.00 SPILLED SPILLED 133.00 130.00 119.00 SPILLED 140.00 133.00 154.00 172.00 131.00 164.00 SPILLED 143.00 128.00 130.00 141.00 150.00 124.00 141.00 146.00 SPILLED 121.00 167.00 151.00 128.00 120.00 SPILLED 121.00 133.00 129.00 190.00 115.00 143.00 142.00 127.00 143.00 SPILLED SPILLED 158.00 SPILLED 132.00 169.00 SPILLED 153.00 SPILLED 128.00 153.00 SPILLED 137.00 147.00 164.00 SPILLED 131.00 175.00 155.00 133.00 128.00 139.00 123.00 132.00 SPILLED SPILLED 111.00 142.00 146.00 129.00 143.00 172.00 139.00 150.00 163.00 SPILLED 145.00 136.00 155.00 128.00 124.00 150.00 SPILLED 125.00 SPILLED 158.00 SPILLED SPILLED 175.00 158.00 126.00 128.00 SPILLED 134.00 135.00 136.00 SPILLED SPILLED 138.00 130.00 NOT TAKEN 162.00 169.00 SPILLED 161.00 SPILLED 136.00 162.00 138.00 165.00 138.00 125.00 SPILLED 183.00 132.00 149.00 173.00 SPILLED SPILLED 185.00 150.00 143.00 132.00 133.00 124.00 138.00 142.00 SPILLED 111.00 SPILLED 140.00 SPILLED 173.00 120.00 133.00 189.00 SPILLED 150.00 SPILLED 154.00 SPILLED 124.00 142.00 SPILLED 127.00 SPILLED 155.00 SPILLED 143.00 171.00 148.00 SPILLED 123.00 152.00 127.00 129.00 139.00 SPILLED 109.00 SPILLED 137.00 SPILLED 159.00 112.00 106.00 161.00 135.00 141.00 SPILLED 162.00 SPILLED 118.00 152.00 SPILLED 134.00 150.00 172.00 150.00 127.00 188.00 141.00 132.00 135.00 116.00 120.00 133.00 139.00 SPILLED 116.00 144.00 137.00 148.00 158.00 150.00 122.00 177.00 124.00 141.00 SPILLED 142.00 SPILLED 135.00 140.00 SPILLED 133.00 149.00 155.00 SPILLED SPILLED 190.00 SPILLED 130.00 129.00 SPILLED 116.00 135.00 134.00 SPILLED 104.00 135.00 137.00 SPILLED 164.00 Covance 6329-183 3M T-6295 PAGE: 14 WEEK 9 WEEK 10 133.00 117.00 149.00 122.00 156.00 140.00 150.00 SPILLED 125.00 143.00 143.00 137.00 159.00 171.00 SPILLED 122.00 180.00 181.00 146.00 133.00 SPILLED 125.00 132.00 134.00 200.00 118.00 146.00 134.00 SPILLED 126.00 SPILLED 132.00 147.00 123.00 SPILLED 130.00 149.00 129.00 134.00 155.00 SPILLED 141.00 147.00 163.00 157.00 131.00 173.00 172.00 138.00 128.00 114.00 SPILLED 132.00 129.00 SPILLED 112.00 130.00 128.00 147.00 152.00 1359 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 GROUP: FEMALE 1 - 0 PPM C92917 C92918 C92919 C92920 C92921 C92922 C92923 C92924 C92925 C92926 C92927 C92928 C92929 C92930 148.00 119.00 SPILLED SPILLED 127.00 136.00 SPILLED 144.00 134.00 143.00 120.00 130.00 114.00 147.00 152.00 121.00 SPILLED 120.00 126.00 156.00 125.00 SPILLED 145.00 SPILLED 190.00 138.00 133.00 179.00 GROUP: FEMALE 2 - 0.5 PPM C92931 C92932 C92933 C92934 C92935 C92936 C92937 C92938 C92939 C92940 C92941 C92942 C92943 C92944 127.00 SPILLED 116.00 117.00 124.00 113.00 116.00 117.00 104.00 114.00 135.00 131.00 132.00 133.00 141.00 SPILLED 128.00 130.00 132.00 125.00 130.00 SPILLED 111.00 126.00 SPILLED SPILLED 137.00 SPILLED SPILLED CNTMD 185.00 131.00 128.00 156.00 SPILLED SPILLED 155.00 152.00 138.00 143.00 132.00 178.00 148.00 SPILLED 129.00 135.00 148.00 135.00 134.00 SPILLED 144.00 140.00 SPILLED SPILLED 155.00 155.00 161.00 115.00 NOT TAKEN SPILLED 130.00 135.00 SPILLED SPILLED 147.00 NOT TAKEN 141.00 157.00 129.00 181.00 172.00 133.00 SPILLED 134.00 153.00 SPILLED SPILLED 165.00 140.00 157.00 139.00 189.00 146.00 SPILLED 145.00 SPILLED 148.00 120.00 133.00 SPILLED 119.00 154.00 NOT TAKEN SPILLED 150.00 167.00 149.00 SPILLED 145.00 132.00 144.00 129.00 136.00 SPILLED 136.00 148.00 SPILLED 161.00 175.00 177.00 127.00 SPILLED 142.00 150.00 SPILLED SPILLED 156.00 145.00 135.00 139.00 182.00 148.00 SPILLED SPILLED SPILLED 129.00 121.00 137.00 SPILLED SPILLED SPILLED SPILLED SPILLED 164.00 158.00 123.00 143.00 148.00 153.00 SPILLED SPILLED 147.00 140.00 138.00 131.00 171.00 145.00 SPILLED 151.00 154.00 135.00 121.00 141.00 SPILLED SPILLED SPILLED 162.00 157.00 163.00 180.00 121.00 SPILLED 138.00 146.00 SPILLED SPILLED SPILLED 122.00 152.00 125.00 165.00 152.00 SPILLED 157.00 129.00 140.00 119.00 SPILLED SPILLED SPILLED SPILLED SPILLED SPILLED 174.00 Covance 6329-183 3M T-6295 PAGE: 15 WEEK 9 WEEK 10 SPILLED 120.00 136.00 144.00 157.00 141.00 178.00 142.00 SPILLED 134.00 134.00 156.00 163.00 124.00 196.00 137.00 SPILLED SPILLED SPILLED 161.00 169.00 139.00 126.00 173.00 143.00 SPILLED 166.00 125.00 135.00 119.00 140.00 SPILLED 127.00 SPILLED 151.00 148.00 167.00 140.00 SPILLED 170.00 SPILLED 133.00 111.00 SPILLED SPILLED 120.00 SPILLED SPILLED 154.00 170.00 1360 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 GROUP: C92945 C92946 C92947 C92948 C92949 C92950 C92951 C92952 C92953 C92954 C92955 C92956 C92957 C92958 C92959 C92960 C92961 C92962 C92963 C92964 C92965 C92966 C92967 C92968 C92969 C92970 C92971 C92972 C92973 C92974 C92975 FEMALE 2 - 0.5 PPM 117.00 98.00 119.00 112.00 SPILLED 127.00 109.00 103.00 113.00 114.00 132.00 123.00 SPILLED 102.00 139.00 SPILLED SPILLED 113.00 102.00 121.00 130.00 107.00 125.00 133.00 107.00 132.00 99.00 113.00 105.00 112.00 127.00 139.00 112.00 128.00 142.00 SPILLED 125.00 120.00 110.00 117.00 132.00 136.00 134.00 130.00 119.00 157.00 183.00 SPILLED 130.00 112.00 186.00 168.00 111.00 136.00 SPILLED 126.00 139.00 112.00 138.00 129.00 115.00 140.00 144.00 116.00 139.00 141.00 202.00 145.00 SPILLED 125.00 SPILLED 130.00 151.00 153.00 138.00 110.00 163.00 SPILLED SPILLED 133.00 115.00 148.00 SPILLED 110.00 148.00 159.00 SPILLED 155.00 123.00 139.00 126.00 123.00 146.00 NOT TAKEN 109.00 142.00 135.00 188.00 NOT TAKEN 114.00 123.00 115.00 135.00 SPILLED 144.00 122.00 123.00 126.00 SPILLED 157.00 114.00 115.00 SPILLED SPILLED 114.00 153.00 SPILLED NOT TAKEN 160.00 117.00 142.00 127.00 120.00 131.00 113.00 155.00 162.00 196.00 133.00 132.00 134.00 151.00 SPILLED 143.00 131.00 127.00 169.00 182.00 160.00 125.00 116.00 SPILLED 150.00 122.00 148.00 161.00 159.00 120.00 144.00 144.00 123.00 155.00 121.00 147.00 139.00 211.00 114.00 118.00 130.00 135.00 SPILLED 144.00 SPILLED 114.00 151.00 SPILLED 149.00 117.00 110.00 SPILLED SPILLED 111.00 164.00 SPILLED 146.00 110.00 139.00 134.00 119.00 142.00 116.00 140.00 151.00 192.00 121.00 127.00 125.00 139.00 143.00 143.00 139.00 117.00 157.00 161.00 153.00 116.00 112.00 SPILLED 144.00 118.00 138.00 144.00 153.00 119.00 136.00 163.00 122.00 153.00 113.00 149.00 162.00 SPILLED 126.00 128.00 SPILLED 136.00 143.00 137.00 SPILLED 121.00 165.00 SPILLED 145.00 124.00 121.00 SPILLED SPILLED 116.00 SPILLED 138.00 161.00 107.00 146.00 138.00 116.00 133.00 Covance 6329-183 3M T-6295 PAGE: 16 WEEK 9 WEEK 10 115.00 139.00 144.00 174.00 126.00 121.00 121.00 130.00 147.00 136.00 SPILLED 114.00 169.00 143.00 141.00 117.00 122.00 119.00 131.00 115.00 130.00 151.00 154.00 112.00 133.00 SPILLED 112.00 139.00 113.00 137.00 130.00 178.00 112.00 131.00 127.00 135.00 131.00 138.00 140.00 106.00 146.00 139.00 159.00 115.00 SPILLED SPILLED 131.00 114.00 129.00 142.00 147.00 110.00 140.00 128.00 117.00 140.00 1361 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 GROUP: FEMALE 2 - 0.5 PPM C92976 C92977 C92978 C92979 C92980 C92981 C92982 C92983 C92984 C92985 C92986 C92987 C92988 C92989 C92990 124.00 116.00 139.00 114.00 113.00 131.00 122.00 125.00 123.00 141.00 116.00 124.00 106.00 153.00 137.00 141.00 120.00 156.00 118.00 129.00 137.00 SPILLED SPILLED 140.00 145.00 134.00 144.00 131.00 SPILLED SPILLED GROUP: FEMALE 3 - 2 PPM C92991 C92992 C92993 C92994 C92995 C92996 C92997 C92998 C92999 C93000 C93001 C93002 C93003 SPILLED 133.00 124.00 114.00 134.00 120.00 117.00 124.00 SPILLED SPILLED 117.00 123.00 174.00 SPILLED 124.00 127.00 110.00 134.00 127.00 119.00 138.00 SPILLED SPILLED 123.00 127.00 SPILLED 155.00 131.00 172.00 127.00 138.00 152.00 SPILLED 131.00 152.00 158.00 142.00 141.00 128.00 165.00 165.00 153.00 126.00 133.00 114.00 147.00 131.00 123.00 155.00 SPILLED 159.00 118.00 133.00 132.00 154.00 133.00 163.00 134.00 141.00 SPILLED NOT TAKEN 145.00 158.00 158.00 134.00 136.00 135.00 TECH SPILL 149.00 162.00 155.00 179.00 134.00 152.00 132.00 SPILLED 147.00 155.00 139.00 136.00 147.00 SPILLED 144.00 SPILLED SPILLED 119.00 124.00 162.00 NOT TAKEN 128.00 137.00 SPILLED SPILLED 140.00 117.00 152.00 SPILLED SPILLED 146.00 121.00 135.00 124.00 153.00 SPILLED 151.00 SPILLED 133.00 160.00 135.00 151.00 168.00 132.00 153.00 SPILLED 166.00 151.00 162.00 140.00 146.00 143.00 213.00 162.00 SPILLED SPILLED 146.00 123.00 140.00 115.00 138.00 SPILLED SPILLED SPILLED 122.00 138.00 138.00 149.00 177.00 132.00 140.00 138.00 167.00 147.00 162.00 136.00 131.00 134.00 SPILLED 152.00 128.00 123.00 132.00 113.00 137.00 121.00 145.00 SPILLED 132.00 118.00 108.00 140.00 139.00 140.00 162.00 122.00 147.00 129.00 SPILLED 151.00 148.00 129.00 138.00 134.00 SPILLED SPILLED 120.00 SPILLED 138.00 124.00 141.00 130.00 143.00 SPILLED SPILLED 129.00 130.00 SPILLED Covance 6329-183 3M T-6295 PAGE: 17 WEEK 9 WEEK 10 137.00 145.00 151.00 124.00 130.00 126.00 SPILLED 138.00 132.00 132.00 126.00 131.00 SPILLED 142.00 136.00 140.00 160.00 126.00 140.00 131.00 141.00 133.00 138.00 131.00 SPILLED 141.00 SPILLED 141.00 118.00 123.00 138.00 121.00 NOT TAKEN 121.00 140.00 SPILLED SPILLED 134.00 109.00 143.00 117.00 125.00 132.00 116.00 SPILLED 145.00 SPILLED SPILLED 138.00 123.00 128.00 1362 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 GROUP: C93004 C93005 C93006 C93007 C93008 C93009 C93010 C93011 C93012 C93013 C93014 C93015 C93016 C93017 C93018 C93019 C93020 C93021 C93022 C93023 C93024 C93025 C93026 C93027 C93028 C93029 C93030 C93031 C93032 C93033 C93034 FEMALE 3 - 2 PPM 127.00 132.00 109.00 SPILLED 141.00 127.00 141.00 135.00 SPILLED SPILLED SPILLED 130.00 80.00 110.00 125.00 127.00 125.00 121.00 120.00 91.00 129.00 137.00 106.00 112.00 110.00 111.00 SPILLED 118.00 117.00 SPILLED 124.00 129.00 129.00 118.00 SPILLED 153.00 117.00 141.00 131.00 SPILLED 116.00 162.00 139.00 99.00 124.00 132.00 133.00 146.00 131.00 143.00 109.00 131.00 141.00 119.00 121.00 122.00 128.00 121.00 124.00 119.00 SPILLED 131.00 168.00 128.00 109.00 SPILLED 156.00 179.00 153.00 139.00 142.00 117.00 SPILLED 148.00 123.00 120.00 141.00 SPILLED 157.00 139.00 131.00 115.00 145.00 165.00 123.00 129.00 125.00 123.00 131.00 146.00 134.00 SPILLED 150.00 131.00 137.00 NOT TAKEN SPILLED NOT TAKEN 129.00 153.00 140.00 164.00 SPILLED 194.00 SPILLED 138.00 140.00 SPILLED SPILLED 141.00 SPILLED 148.00 111.00 148.00 151.00 134.00 136.00 126.00 SPILLED 139.00 NOT TAKEN 119.00 SPILLED 124.00 131.00 134.00 141.00 133.00 146.00 138.00 160.00 SPILLED 177.00 140.00 129.00 137.00 144.00 SPILLED 143.00 141.00 151.00 120.00 142.00 139.00 144.00 142.00 SPILLED SPILLED 131.00 133.00 SPILLED 148.00 133.00 129.00 SPILLED SPILLED 147.00 SPILLED 143.00 SPILLED 164.00 SPILLED 121.00 150.00 140.00 SPILLED SPILLED 140.00 135.00 SPILLED 141.00 162.00 135.00 151.00 SPILLED SPILLED 129.00 125.00 SPILLED 135.00 133.00 120.00 SPILLED 127.00 138.00 119.00 155.00 119.00 SPILLED 144.00 121.00 136.00 131.00 SPILLED 147.00 136.00 140.00 SPILLED 136.00 147.00 130.00 135.00 133.00 SPILLED 116.00 121.00 SPILLED 118.00 128.00 131.00 SPILLED 132.00 139.00 128.00 139.00 119.00 139.00 SPILLED 116.00 139.00 131.00 152.00 SPILLED 135.00 136.00 117.00 135.00 159.00 130.00 134.00 129.00 SPILLED 128.00 126.00 160.00 125.00 Covance 6329-183 3M T-6295 PAGE: 18 WEEK 9 WEEK 10 124.00 111.00 SPILLED 135.00 136.00 123.00 141.00 104.00 136.00 SPILLED 117.00 140.00 126.00 SPILLED 134.00 124.00 131.00 SPILLED 125.00 SPILLED SPILLED 128.00 132.00 SPILLED 134.00 118.00 SPILLED 131.00 131.00 123.00 SPILLED SPILLED 139.00 118.00 135.00 120.00 146.00 157.00 116.00 SPILLED 134.00 SPILLED SPILLED 138.00 141.00 SPILLED 132.00 155.00 143.00 125.00 134.00 SPILLED SPILLED 125.00 SPILLED 136.00 1363 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 GROUP: FEMALE 3 - 2 PPM C93035 C93036 C93037 C93038 C93039 C93040 C93041 C93042 C93043 C93044 C93045 C93046 C93047 C93048 C93049 C93050 SPILLED 111.00 119.00 130.00 119.00 SPILLED 132.00 128.00 118.00 123.00 SPILLED 142.00 147.00 118.00 116.00 118.00 143.00 134.00 126.00 117.00 136.00 SPILLED 146.00 130.00 127.00 SPILLED 222.00 156.00 154.00 121.00 125.00 129.00 GROUP: FEMALE 4 - 5 PPM C93051 C93052 C93053 C93054 C93055 C93056 C93057 C93058 C93059 C93060 C93061 C93062 125.00 129.00 115.00 SPILLED 136.00 129.00 136.00 151.00 135.00 135.00 116.00 112.00 SPILLED SPILLED 116.00 SPILLED 130.00 SPILLED 124.00 153.00 136.00 160.00 123.00 117.00 186.00 SPILLED 134.00 144.00 155.00 173.00 161.00 143.00 131.00 SPILLED SPILLED SPILLED 148.00 119.00 123.00 148.00 152.00 142.00 128.00 126.00 139.00 133.00 141.00 159.00 148.00 148.00 131.00 124.00 152.00 125.00 141.00 SPILLED 144.00 SPILLED 150.00 159.00 131.00 136.00 NOT TAKEN 182.00 210.00 133.00 122.00 163.00 165.00 155.00 146.00 SPILLED 151.00 SPILLED SPILLED 164.00 148.00 128.00 153.00 187.00 127.00 129.00 157.00 70.00 129.00 144.00 SPILLED 149.00 SPILLED SPILLED 142.00 143.00 117.00 207.00 SPILLED 127.00 121.00 150.00 SPILLED SPILLED 121.00 125.00 145.00 135.00 133.00 NOT TAKEN 138.00 135.00 123.00 132.00 SPILLED SPILLED 137.00 SPILLED 145.00 141.00 136.00 SPILLED SPILLED 119.00 SPILLED 135.00 134.00 128.00 144.00 TECH SPILL 130.00 130.00 132.00 135.00 131.00 123.00 142.00 129.00 137.00 121.00 143.00 SPILLED 151.00 141.00 143.00 126.00 SPILLED 161.00 SPILLED 118.00 152.00 129.00 SPILLED 136.00 SPILLED 130.00 133.00 127.00 139.00 146.00 129.00 129.00 160.00 134.00 126.00 141.00 145.00 SPILLED 149.00 147.00 133.00 SPILLED SPILLED 152.00 125.00 SPILLED 153.00 140.00 SPILLED 141.00 SPILLED SPILLED 128.00 117.00 132.00 137.00 132.00 129.00 Covance 6329-183 3M T-6295 PAGE: 19 WEEK 9 WEEK 10 SPILLED SPILLED 134.00 141.00 146.00 208.00 164.00 127.00 136.00 118.00 157.00 137.00 118.00 133.00 SPILLED 138.00 135.00 124.00 SPILLED 151.00 SPILLED 154.00 137.00 134.00 126.00 148.00 144.00 132.00 127.00 SPILLED 129.00 SPILLED 143.00 SPILLED 144.00 138.00 124.00 130.00 128.00 121.00 125.00 171.00 SPILLED 133.00 SPILLED SPILLED 130.00 131.00 133.00 122.00 SPILLED 123.00 1364 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 GROUP: C93063 C93064 C93065 C93066 C93067 C93068 C93069 C93070 C93071 C93072 C93073 C93074 C93075 C93076 C93077 C93078 C93079 C93080 C93081 C93082 C93083 C93084 C93085 C93086 C93087 C93088 C93089 C93090 C93091 C93092 C93093 FEMALE 4 - 5 PPM 118.00 118.00 112.00 121.00 122.00 122.00 149.00 151.00 110.00 125.00 139.00 138.00 123.00 124.00 123.00 128.00 139.00 129.00 125.00 SPILLED 133.00 115.00 129.00 148.00 SPILLED 131.00 157.00 SPILLED 133.00 130.00 146.00 134.00 125.00 112.00 121.00 119.00 SPILLED 188.00 SPILLED 106.00 138.00 138.00 167.00 123.00 127.00 127.00 136.00 190.00 133.00 113.00 SPILLED 133.00 119.00 SPILLED SPILLED 144.00 126.00 150.00 SPILLED 135.00 129.00 136.00 128.00 131.00 120.00 138.00 149.00 138.00 172.00 168.00 115.00 145.00 146.00 180.00 136.00 160.00 140.00 136.00 143.00 136.00 124.00 SPILLED 140.00 126.00 141.00 SPILLED 159.00 133.00 156.00 SPILLED 132.00 143.00 140.00 SPILLED 126.00 125.00 124.00 NOT TAKEN 124.00 161.00 NOT TAKEN 110.00 SPILLED 143.00 138.00 NOT TAKEN 139.00 132.00 142.00 167.00 130.00 116.00 SPILLED 134.00 NOT TAKEN 133.00 141.00 120.00 130.00 204.00 SPILLED 151.00 SPILLED 143.00 139.00 126.00 119.00 SPILLED 136.00 159.00 106.00 SPILLED 155.00 SPILLED 156.00 131.00 151.00 154.00 152.00 123.00 SPILLED 168.00 163.00 151.00 SPILLED 132.00 159.00 SPILLED 128.00 134.00 154.00 122.00 119.00 116.00 SPILLED 122.00 143.00 105.00 SPILLED 138.00 165.00 SPILLED 129.00 146.00 147.00 128.00 126.00 SPILLED 142.00 133.00 130.00 123.00 118.00 SPILLED SPILLED 136.00 139.00 SPILLED 118.00 126.00 117.00 SPILLED 122.00 143.00 114.00 SPILLED 146.00 149.00 146.00 138.00 141.00 161.00 120.00 120.00 156.00 144.00 SPILLED 137.00 125.00 129.00 SPILLED 123.00 133.00 134.00 158.00 115.00 132.00 119.00 SPILLED 128.00 144.00 113.00 SPILLED 142.00 147.00 138.00 132.00 143.00 193.00 122.00 127.00 SPILLED 142.00 124.00 SPILLED SPILLED 116.00 156.00 122.00 121.00 127.00 156.00 Covance 6329-183 3M T-6295 PAGE: 20 WEEK 9 WEEK 10 114.00 131.00 SPILLED 135.00 129.00 151.00 114.00 SPILLED 144.00 150.00 140.00 125.00 142.00 140.00 124.00 117.00 SPILLED 152.00 129.00 SPILLED 109.00 119.00 143.00 111.00 134.00 130.00 166.00 126.00 128.00 119.00 127.00 128.00 137.00 115.00 154.00 134.00 160.00 146.00 126.00 148.00 148.00 111.00 135.00 143.00 135.00 SPILLED 137.30 SPILLED 135.00 155.00 123.00 135.00 135.00 158.00 1365 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 GROUP: FEMALE 4 - 5 PPM C93094 C93095 C93096 C93097 C93098 C93099 C93100 C93101 C93102 C93103 C93104 C93105 C93106 C93107 C93108 C93109 C93110 134.00 134.00 146.00 109.00 128.00 123.00 156.00 111.00 152.00 126.00 141.00 171.00 119.00 121.00 121.00 121.00 SPILLED 161.00 128.00 SPILLED 114.00 SPILLED 132.00 162.00 SPILLED 159.00 142.00 155.00 153.00 124.00 125.00 127.00 125.00 180.00 GROUP: FEMALE 5 - 20 PPM C93111 C93112 C93113 C93114 C93115 C93116 C93117 C93118 C93119 C93120 C93121 137.00 137.00 SPILLED 126.00 108.00 118.00 113.00 108.00 118.00 131.00 SPILLED 152.00 142.00 SPILLED 140.00 118.00 121.00 117.00 121.00 112.00 133.00 105.00 142.00 138.00 177.00 120.00 146.00 138.00 158.00 121.00 159.00 139.00 162.00 154.00 124.00 SPILLED 122.00 129.00 SPILLED 159.00 137.00 SPILLED 135.00 118.00 124.00 SPILLED 124.00 113.00 128.00 106.00 191.00 141.00 SPILLED 132.00 SPILLED 131.00 162.00 122.00 155.00 128.00 166.00 143.00 124.00 136.00 122.00 132.00 SPILLED 135.00 155.00 181.00 132.00 127.00 129.00 150.00 124.00 170.00 151.00 184.00 170.00 130.00 136.00 124.00 138.00 207.00 149.00 132.00 119.00 NOT TAKEN 112.00 126.00 122.00 126.00 116.00 122.00 97.00 145.00 135.00 120.00 130.00 SPILLED SPILLED 128.00 116.00 SPILLED SPILLED 171.00 SPILLED 171.00 128.00 128.00 120.00 SPILLED 129.00 156.00 139.00 SPILLED 133.00 125.00 135.00 118.00 126.00 199.00 136.00 129.00 110.00 109.00 121.00 SPILLED 110.00 106.00 124.00 SPILLED 137.00 SPILLED 169.00 136.00 121.00 119.00 148.00 SPILLED 158.00 124.00 204.00 140.00 126.00 138.00 127.00 132.00 SPILLED 141.00 125.00 119.00 111.00 SPILLED 121.00 123.00 106.00 120.00 108.00 128.00 SPILLED 161.00 SPILLED SPILLED 127.00 144.00 125.00 165.00 143.00 SPILLED 139.00 117.00 132.00 125.00 133.00 SPILLED 136.00 119.00 124.00 114.00 SPILLED 122.00 113.00 96.00 122.00 99.00 Covance 6329-183 3M T-6295 PAGE: 21 WEEK 9 WEEK 10 135.00 124.00 158.00 SPILLED SPILLED 116.00 150.00 123.00 157.00 138.00 208.00 132.00 117.00 122.00 128.00 126.00 SPILLED 137.00 127.00 168.00 SPILLED 136.00 115.00 151.00 119.00 SPILLED 128.00 SPILLED 153.00 129.00 127.00 136.00 130.00 SPILLED 128.00 111.00 120.00 107.00 116.00 SPILLED 118.00 101.00 107.00 98.00 SPILLED 124.00 129.00 115.00 122.00 123.00 119.00 107.00 124.00 103.00 1366 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 GROUP: C93122 C93123 C93124 C93125 C93126 C93127 C93128 C93129 C93130 C93131 C93132 C93133 C93134 C93135 C93136 C93137 C93138 C93139 C93140 C93141 C93142 C93143 C93144 C93145 C93146 C93147 C93148 C93149 C93150 C93151 C93152 FEMALE 5 - 20 PPM 134.00 127.00 SPILLED 104.00 103.00 125.00 106.00 128.00 128.00 133.00 130.00 129.00 139.00 136.00 125.00 124.00 117.00 133.00 130.00 130.00 149.00 132.00 SPILLED 110.00 115.00 118.00 126.00 113.00 160.00 126.00 133.00 151.00 150.00 SPILLED 118.00 104.00 122.00 126.00 136.00 137.00 131.00 129.00 124.00 126.00 132.00 118.00 143.00 122.00 137.00 140.00 SPILLED 135.00 145.00 122.00 110.00 105.00 124.00 126.00 SPILLED 171.00 123.00 146.00 SPILLED 131.00 151.00 120.00 SPILLED 123.00 114.00 146.00 133.00 135.00 129.00 124.00 124.00 140.00 111.00 141.00 121.00 SPILLED 153.00 162.00 142.00 SPILLED 125.00 SPILLED 105.00 122.00 118.00 134.00 160.00 122.00 SPILLED 134.00 NOT TAKEN SPILLED 119.00 103.00 134.00 121.00 147.00 149.00 127.00 120.00 118.00 128.00 137.00 116.00 120.00 121.00 124.00 138.00 SPILLED NOT TAKEN SPILLED NOT TAKEN 128.00 106.00 128.00 116.00 133.00 159.00 111.00 128.00 157.00 SPILLED 122.00 108.00 125.00 119.00 146.00 144.00 131.00 123.00 120.00 132.00 147.00 121.00 136.00 126.00 134.00 152.00 182.00 153.00 SPILLED SPILLED 132.00 126.00 154.00 163.00 129.00 126.00 124.00 SPILLED 117.00 101.00 108.00 113.00 139.00 124.00 130.00 112.00 105.00 134.00 129.00 115.00 128.00 117.00 SPILLED 138.00 139.00 SPILLED 130.00 SPILLED 129.00 129.00 SPILLED 142.00 122.00 136.00 125.00 127.00 SPILLED 133.00 126.00 122.00 134.00 141.00 122.00 116.00 SPILLED 125.00 134.00 113.00 132.00 123.00 117.00 136.00 SPILLED 158.00 123.00 125.00 128.00 147.00 SPILLED 139.00 124.00 SPILLED SPILLED 109.00 124.00 101.00 128.00 115.00 131.00 138.00 124.00 106.00 113.00 128.00 131.00 120.00 121.00 119.00 111.00 145.00 166.00 SPILLED SPILLED 105.00 118.00 135.00 150.00 147.00 123.00 129.00 Covance 6329-183 3M T-6295 PAGE: 22 WEEK 9 WEEK 10 138.00 118.00 104.00 100.00 111.00 120.00 134.00 131.00 124.00 115.00 112.00 117.00 131.00 114.00 114.00 122.00 116.00 126.00 SPILLED 137.00 123.00 SPILLED 119.00 136.00 155.00 152.00 133.00 128.00 130.00 138.00 122.00 102.00 121.00 116.00 125.00 133.00 117.00 126.00 116.00 127.00 127.00 126.00 126.00 122.00 125.00 138.00 145.00 SPILLED 115.00 118.00 116.00 132.00 SPILLED 149.00 123.00 123.00 1367 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 GROUP: C93153 C93154 C93155 C93156 C93157 C93158 C93159 C93160 C93161 C93162 C93163 C93164 C93165 C93166 C93167 C93168 C93169 C93170 C93171 C93172 C93173 C93174 C93175 C93176 C93177 C93178 C93179 C93180 FEMALE 5 - 20 PPM SPILLED 130.00 131.00 115.00 130.00 124.00 118.00 140.00 119.00 149.00 111.00 133.00 124.00 129.00 110.00 129.00 122.00 122.00 SPILLED 120.00 118.00 119.00 118.00 112.00 118.00 SPILLED 115.00 SPILLED SPILLED 136.00 145.00 117.00 123.00 128.00 114.00 148.00 SPILLED 159.00 121.00 125.00 129.00 132.00 135.00 149.00 127.00 130.00 SPILLED 116.00 133.00 SPILLED 133.00 117.00 117.00 127.00 116.00 153.00 152.00 130.00 152.00 118.00 145.00 122.00 118.00 151.00 SPILLED 169.00 SPILLED 109.00 128.00 135.00 133.00 136.00 126.00 137.00 SPILLED 119.00 142.00 SPILLED 127.00 124.00 118.00 134.00 115.00 SPILLED SPILLED 128.00 115.00 109.00 126.00 118.00 NOT TAKEN 148.00 136.00 141.00 118.00 SPILLED 122.00 145.00 122.00 139.00 137.00 132.00 SPILLED SPILLED 109.00 125.00 114.00 122.00 115.00 137.00 114.00 138.00 SPILLED 137.00 131.00 118.00 137.00 137.00 161.00 138.00 146.00 120.00 SPILLED 135.00 142.00 138.00 146.00 134.00 153.00 SPILLED 123.00 125.00 135.00 127.00 129.00 118.00 126.00 120.00 148.00 SPILLED 129.00 128.00 111.00 134.00 116.00 146.00 SPILLED 149.00 120.00 118.00 126.00 140.00 122.00 141.00 120.00 133.00 SPILLED 119.00 116.00 123.00 111.00 116.00 111.00 121.00 114.00 137.00 SPILLED 129.00 147.00 118.00 134.00 111.00 147.00 SPILLED 138.00 115.00 116.00 121.00 SPILLED 120.00 132.00 123.00 130.00 SPILLED 130.00 SPILLED SPILLED 125.00 120.00 119.00 123.00 113.00 135.00 SPILLED 127.00 159.00 112.00 125.00 126.00 148.00 130.00 SPILLED 106.00 SPILLED 115.00 139.00 131.00 123.00 117.00 SPILLED SPILLED 115.00 SPILLED SPILLED 109.00 113.00 111.00 112.00 111.00 139.00 Covance 6329-183 3M T-6295 PAGE: 23 WEEK 9 WEEK 10 SPILLED 126.00 130.00 107.00 131.00 123.00 144.00 SPILLED 146.00 107.00 116.00 119.00 127.00 111.00 116.00 121.00 140.00 SPILLED SPILLED 130.00 SPILLED 118.00 113.00 134.00 118.00 113.00 SPILLED SPILLED 130.00 SPILLED 111.00 133.00 129.00 144.00 128.00 SPILLED 113.00 112.00 117.00 134.00 123.00 SPILLED 112.00 SPILLED SPILLED 128.00 SPILLED SPILLED 119.00 113.00 127.00 116.00 SPILLED 140.00 1368 Covance 6329-183 3M T-6295 ffftttorH (N H O Cft ft f t (?) ft oO O O O O O O O O O O O O O O O O O O O O O O O O O O oO O O O O O O O O O O O O O O O O O n O n O O O O O O n O O ft ftrH O l O (?) O [> ro LO O l rH rH LO LO LO ro CO ww ro rH O l LO LO [> w CO rH O l ft ftro rH rH O l rH O l O l ro LO rH O l [> O l rH O O l rH rH O l ro ro O l rH O f t O l O l ftrH rH rH rH rH rH rH rH rH rH rH rH rH rH rH rH rH rH rH H rH H rH rH rH rH rH rH H rH rH ft ft ui ui ui OOOOOOOOOOOOOOOOOOOOO OOO OOOOO OOOOOOOOOOOOOOOOOOOOOn OOOn OOOOO ft(?) O LO ro l> o ro ro Li) l> rH Li) O LO CO LO O rH rH l> w ft O l Li) O w ft Li) O l> O l O l O l rH ro O O l O l O l O l ro O O LO LO rH O O l rH O rH f t ro O l ro rH O O l O l O l futi futirH rH rH rH rH rH rH rH rH rH rH rH rH rH rH rH rH rH rH O l rH H rH rH rH H rH rH rH rH rH tn rd jj rd o -H JJ 3 CO TJ a) O U ft T J ft ftC O O rd -H TJ Cl 1 1 1 1 UI 1 1 Eh i <ft ii i M i1 1 - -1 LO 1 (?) 1 Ol 1 LO 1 XI Eh Eh 1 ft 1 H co i Hi ii i i i i i i i ft i W l> i Hi ii oOOOOOOOOOOOOO OOOO OO OO OO oOOOOOOOOOOOOOnOOOOnnnnOOnOOnOO ft ftftftft ftO l l> Li) O (?) l> CO Li) O rH LO LO CO w O l (?) CO Li) w w w w CO CO w (?) wft [> CM ftftro O O l O l O rH O l O l O l LO O rH ro ft rH (?)O rH ft ft CM CM ft CM O ft CM rH rH rH rH rH rH rH rH rH rH rH rH rH rH rH H rH rH rH H M M M rH rH H rH rH H rH rH futi futi futi futi futi futi futi OOOOOOOOOOOOOOOOOOO OOOOOOOOOOO OOOOOOOOOO O O O O O O O O O n O O O O O O O O O O O ftO (?) CO ro ro l> ftro rH O l O l ro O rH Li) l> CO LO O ro Li) LO O Li) O l (?) w ro O l O l O l O l O l LO O l O l O O l O l> O l> O CM CM (?) Li) ro ro ro rH CO rH O CM l> o LO CM O rH rH rH rH rH rH rH rH rH rH rH rH rH rH rH rH rH rH rH rH H rH rH rH rH rH rH rH rH rH rH rH H Ul O W ft CD f t O-- H E-l U ft f t ri ri U I U 2 SQ MP <! w >h rwi Eh Eh MO U ft M XQ OM Eh U c U MU m OS ft O 1 1 1 1 1 1 1 1 i I ffi f t UJ ftX PU } ri H Eh S H ri M Eh U O ft O H ft HO PP iJ oF fftt HH ft ft H LO H i i i i 1 i ii i i 1 1 X1 W LO 1 W1 ii i i i i i i i X1 Hi Wi ii oO O O O O O O O O O O O O O OOOOO OOO OO O oO O O O O O O O O O O O O O oO O O O oO O oO o ft ft ft ftftO I [> LO [> ro (?) LO O O LO CO rH LO LO w rH LO (?) LO (?) w ftro O ro rH rH O I O I O I O I ro rH ro O I f t O O I rH O I O f t O W o O w H LO ro ro rH O f t f t O ft ft ft ftftrH rH rH rH rH rH rH rH rH rH rH rH rH rH rH M rH rH rH rH rH M rH rH rH M rH rH M M rH Ul Ul Ul Ul Ul o OOO OOO OOOOOO OOOOOOOOOOOO OO o OOOOOOOOOOOOOOOOOOOOOOOOOO ft ftLO LO O I (?) w Ol OI O W w LO O I rH r o r o rH f t O l CO rH O I O I [> CO ftr o O I r o O I rH O I r o f t r o O I r o LO r o f t O r o O I LO O I O I O I ftLO LO LO O w CO [> ro OI OI OI f t ro rH ftrH rH rH rH M rH rH rH M rH rH rH rH rH rH M rH rH rH rH rH rH rH rH rH rH rH rH M rH rH ft ft ft Ul Ul Ul Ul OOOO OOO OOOOOO OOOOOOOOOOOO OO OOOOOOOOOOOOOOOOOOOOOOOOOOO (?) ro [> [> H ft LO O H ro f t OI ro (?) O I [> (?) H ft LO O CO r o (?) rH O H rH LO CO O I f t rH LO rH O I O I O I f t rH rH f t O I O I r o LO O I f t O rH rH OI r o O rH rH f t LO rH rH rH rH rH M rH rH rH M rH rH rH rH rH rH M rH rH rH rH rH rH rH rH rH rH rH rH M rH rH 1 1 X1 H ro i Wi i i i i i o OOO OOO OOOOOO OOOOOOOOOOOO OO o OOOo OOo OOOOOo OOOOOOOOOOOo O ww W w ft ftLOOI ro ft ro (?) O ft o (?) (?) COOI ro ro rHLOOI O O O [> O ro CO LOLO ft ft ftOI ro ro OI OI rH ro OI O ro LO o ro OI ro ro OI OI LOrHOI OI ft OI rH rH rHrH rH M rH rHrH M rH rH rHrH rHrH M rH rH rHrH rH rH rH rH rH rHrH rH M rH rH ft ft ft ft Ul Ul Ul Ul X W OI W o OOOOOOO OOOOO OOOOOOOOo o OOOO o OOOOOOOOOOOOOOOOOOOOo o OOOO ft ft fftt fftt ft2 Ol rH CO [> Ol (?) w ft rH rH [> ro W O l [> CO LO rH rH O CO w O l CO CO r o CO w O l O l O l O LO rH r o O l O l LO rH r o r o r o O O l rH O l O l O l r o r o rH O l rH O l O l f t ft ftf t rH rH rH rH rH rH rH rH M rH rH rH rH rH M rH rH rH rH rH rH rH rH rH M rH rH rH rH rH M ft ft Ul Ul Ul Ul 20 LO O O O O OOO OOOOOo OOOOO OOo o o OOOOo X OOOOOOOOOOOOo OOOOOOOo o o OOOOo WH ft ft ft ftW ft ft H (?) (?) LO w LO O w CO O l LO LO O O l O l LO CO [> r o w O O r o [> [> O l O l rH rH rH C 2 O l rH O l rH rH O l r o r o rH O l r o LO rH O O l O l r o f t O r o r o O l O l O l O l O rH rH rH rH M rH rH rH M rH rH rH rH rH rH rH rH rH rH rH M rH rH rH rH rH rH rH rH rH rH 1369 f t ft 1 CW 1 2i--i fSt ii < P i i ft P ft rH O l ro LT) LO [> CO (?)o rH O l ro LO LO [> CO (?)o rH O l ro LO LO [> CO (?)o rH CD CO CO CO CO CO CO CO CO CO (?)(?)(?)(?)(?)(?)(?)(?)cd (?)o O o o o o o o o o rH rH rH rH rH rH rH rH rH rH rH rH rH rH rH rH rH rH rH rH rH O l O l O l O l O l O l O l O l O l O l O l O l ro ro ro ro ro ro ro ro ro ro ro ro ro ro ro ro ro ro ro ro ro ro ro ro ro ro ro ro ro ro ro (?)(?)(?)(?)(?)(?)(?)(?)(?)(?)(?)(?)(?)(?)(?)(?)(?)(?)(?)(?)(?)(?)(?)(?)(?)(?)(?)O) (?)(?)(?) UUUUUUUUUUUUUUUUUUUUUUUUUUUUUUu Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 GROUP: C93212 C93213 C93214 C93215 C93216 C93217 C93218 C93219 C93220 FEMALE 6 - 20 PPM 114.00 121.00 116.00 145.00 113.00 SPILLED 127.00 141.00 SPILLED 135.00 123.00 117.00 124.00 109.00 SPILLED 117.00 151.00 SPILLED 131.00 130.00 113.00 SPILLED 118.00 SPILLED 139.00 161.00 SPILLED 137.00 126.00 SPILLED 128.00 115.00 SPILLED 119.00 153.00 SPILLED 141.00 125.00 SPILLED 133.00 122.00 SPILLED 124.00 155.00 SPILLED 125.00 119.00 SPILLED 126.00 114.00 153.00 SPILLED SPILLED SPILLED 140.00 117.00 117.00 122.00 114.00 145.00 SPILLED SPILLED SPILLED 133.00 113.00 114.00 125.00 110.00 132.00 SPILLED 139.00 SPILLED Covance 6329-183 3M T-6295 PAGE: 25 WEEK 9 WEEK 10 135.00 116.00 119.00 119.00 118.00 130.00 SPILLED 129.00 SPILLED 142.0 0 117.0 0 120.0 0 121.0 0 112.0 0 137.0 0 SPILLED SPILLED SPILLED 1370 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 11 WEEK 12 WEEK 13 WEEK 14 WEEK 15 WEEK 16 WEEK 20 WEEK 24 GROUP: C92501 C92502 C92503 C92504 C92505 C92507 C92508 C92509 C92510 C92511 C92512 C92513 C92514 C92515 C92516 C92517 C92518 C92519 C92520 C92521 C92522 C92523 C92525 C92527 C92528 C92530 C92531 C92532 C92533 C92534 C92535 C92536 MALE 1 - 0 SPILLED 182.00 187.00 190.00 194.00 184.00 180.00 178.00 202.00 135.00 191.00 187.00 211.00 187.00 167.00 217.00 203.00 168.00 192.00 184.00 166.00 174.00 185.00 189.00 175.00 SPILLED SPILLED SPILLED 212.00 180.00 203.00 175.00 PPM 223.00 174.00 177.00 118.00 SPILLED 171.00 173.00 179.00 207.00 SPILLED 190.00 177.00 213.00 185.00 173.00 204.00 200.00 173.00 199.00 187.00 SPILLED 172.00 184.00 189.00 SPILLED SPILLED SPILLED 236.00 204.00 169.00 193.00 178.00 221.00 165.00 177.00 163.00 186.00 180.00 169.00 172.00 203.00 231.00 182.00 170.00 206.00 173.00 162.00 191.00 206.00 162.00 189.00 185.00 157.00 169.00 174.00 181.00 169.00 SPILLED SPILLED 234.00 199.00 165.00 187.00 188.00 222.00 174.00 175.00 165.00 190.00 175.00 153.00 NOT TAKEN 207.00 NOT TAKEN 183.00 174.00 220.00 181.00 161.00 197.00 205.00 165.00 192.00 NOT TAKEN 156.00 166.00 181.00 157.00 NOT TAKEN SPILLED 207.00 NOT TAKEN 194.00 175.00 181.00 186.00 217.00 181.00 178.00 173.00 194.00 174.00 177.00 194.00 196.00 176.00 218.00 190.00 160.00 203.00 206.00 168.00 205.00 160.00 175.00 187.00 187.00 SPILLED SPILLED 203.00 173.00 209.00 186.00 213.00 179.00 185.00 169.00 201.00 171.00 183.00 SPILLED 192.00 181.00 209.00 181.00 172.00 212.00 207.00 171.00 204.00 162.00 174.00 186.00 188.00 SPILLED SPILLED 201.00 172.00 199.00 185.00 214.00 179.00 165.00 187.00 191.00 168.00 187.00 192.00 199.00 184.00 226.00 178.00 164.00 216.00 232.00 163.00 196.00 162.00 188.00 186.00 188.00 SPILLED 219.00 195.00 173.00 190.00 196.00 194.00 161.00 169.00 188.00 172.00 162.00 170.00 173.00 189.00 182.00 200.00 166.00 163.00 204.00 212.00 153.00 182.00 140.00 181.00 181.00 174.00 SPILLED 212.00 186.00 190.00 173.00 175.00 Covance 6329-183 3M T-6295 PAGE: 26 WEEK 28 WEEK 32 197.00 157.00 173.00 162.00 174.00 160.00 180.00 170.00 184.00 179.00 199.00 160.00 165.00 210.00 208.00 152.00 175.00 152.00 176.00 169.00 175.00 SPILLED 198.00 194.00 181.00 174.00 186.00 188.00 168.00 170.00 159.00 179.00 158.00 169.00 188.00 169.00 171.00 188.00 153.00 178.00 190.00 203.00 156.00 174.00 158.00 167.00 166.00 170.00 SPILLED 169.00 178.00 171.00 187.00 169.00 1371 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 11 WEEK 12 WEEK 13 WEEK 14 WEEK 15 WEEK 16 WEEK 20 WEEK 24 GROUP: C92537 C92538 C92539 C92540 C92541 C92542 C92543 C92544 C92545 C92547 C92548 C92549 C92550 C92551 C92552 C92553 C92554 C92555 C92556 C92557 C92558 C92559 C92560 C92561 C92562 C92563 C92564 C92565 C92566 C92567 C92568 MALE 1 - 0 PPM 212.00 193.00 218.00 210.00 198.00 180.00 170.00 211.00 201.00 201.00 177.00 163.00 205.00 165.00 193.00 148.00 162.00 205.00 176.00 202.00 203.00 181.00 185.00 182.00 193.00 SPILLED 193.00 209.00 193.00 179.00 198.00 219.00 203.00 211.00 208.00 203.00 184.00 172.00 208.00 184.00 199.00 176.00 162.00 204.00 172.00 193.00 149.00 157.00 215.00 182.00 SPILLED 202.00 178.00 184.00 187.00 SPILLED 225.00 203.00 200.00 195.00 175.00 195.00 212.00 204.00 215.00 198.00 190.00 184.00 166.00 190.00 182.00 196.00 172.00 157.00 208.00 163.00 189.00 143.00 151.00 215.00 171.00 195.00 200.00 175.00 191.00 174.00 SPILLED SPILLED 199.00 SPILLED 189.00 185.00 187.00 189.00 205.00 215.00 203.00 191.00 181.00 172.00 197.00 187.00 191.00 181.00 163.00 208.00 164.00 204.00 146.00 158.00 214.00 177.00 198.00 185.00 178.00 182.00 181.00 170.00 202.00 197.00 187.00 174.00 180.00 184.00 212.00 196.00 215.00 205.00 192.00 189.00 173.00 201.00 199.00 194.00 185.00 166.00 217.00 172.00 194.00 146.00 161.00 211.00 178.00 203.00 211.00 182.00 192.00 175.00 190.00 222.00 197.00 197.00 189.00 177.00 186.00 217.00 194.00 218.00 208.00 197.00 184.00 167.00 205.00 198.00 196.00 182.00 170.00 217.00 169.00 196.00 146.00 161.00 211.00 184.00 187.00 209.00 180.00 194.00 176.00 198.00 215.00 203.00 196.00 191.00 167.00 194.00 227.00 209.00 227.00 214.00 201.00 190.00 173.00 197.00 187.00 193.00 SPILLED 161.00 221.00 174.00 191.00 137.00 163.00 222.00 180.00 199.00 205.00 178.00 198.00 187.00 185.00 228.00 201.00 197.00 182.00 191.00 196.00 212.00 210.00 219.00 217.00 202.00 192.00 168.00 185.00 188.00 184.00 179.00 168.00 217.00 163.00 192.00 127.00 154.00 217.00 168.00 212.00 196.00 177.00 194.00 180.00 184.00 215.00 195.00 208.00 171.00 174.00 Covance 6329-183 3M T-6295 PAGE: 27 WEEK 28 WEEK 32 204.00 218.00 211.00 211.00 193.00 SPILLED 170.00 SPILLED 180.00 176.00 176.00 161.00 219.00 162.00 195.00 119.00 147.00 195.00 182.00 183.00 201.00 182.00 208.00 173.00 196.00 224.00 202.00 208.00 176.00 186.00 210.00 193.00 204.00 200.00 180.00 170.00 166.00 180.00 181.00 173.00 179.00 150.00 209.00 159.00 186.00 126.00 142.00 193.00 166.00 174.00 196.00 168.00 191.00 178.00 176.00 217.00 188.00 185.00 173.00 190.00 1372 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 11 WEEK 12 WEEK 13 WEEK 14 WEEK 15 WEEK 16 WEEK 20 WEEK 24 GROUP: C92569 C92570 GROUP: C92571 C92572 C92573 C92576 C92577 C92578 C92579 C92580 C92581 C92582 C92583 C92584 C92585 C92586 C92587 C92588 C92589 C92590 C92591 C92592 C92593 C92594 C92595 C92596 C92597 C92598 MALE 1 - 0 PPM 177.00 199.00 181.00 184.00 MALE 2 - 0 .5 PPM 220.00 200.00 204.00 186.00 198.00 196.00 207.00 192.00 183.00 156.00 174.00 165.00 CNTMD 179.00 199.00 SPILLED 203.00 204.00 166.00 178.00 175.00 196.00 TECH SPILL 195.00 SPILLED 186.00 221.00 204.00 208.00 190.00 203.00 190.00 206.00 200.00 190.00 157.00 169.00 175.00 CNTMD 181.00 197.00 181.00 192.00 213.00 166.00 173.00 166.00 194.00 173.00 191.00 182.00 183.00 168.00 192.00 176.00 194.00 193.00 188.00 215.00 196.00 201.00 192.00 199.00 192.00 205.00 194.00 190.00 148.00 160.00 176.00 202.00 189.00 198.00 182.00 189.00 212.00 164.00 169.00 164.00 186.00 164.00 195.00 185.00 181.00 212.00 205.00 198.00 186.00 199.00 200.00 194.00 192.00 185.00 150.00 145.00 167.00 186.00 194.00 206.00 183.00 194.00 211.00 166.00 182.00 NOT TAKEN 185.00 169.00 190.00 179.00 159.00 217.00 200.00 207.00 190.00 195.00 192.00 200.00 190.00 184.00 156.00 181.00 179.00 CNTMD 184.00 205.00 197.00 193.00 214.00 165.00 175.00 195.00 182.00 189.00 191.00 176.00 194.00 195.00 195.00 198.00 199.00 199.00 207.00 198.00 209.00 196.00 195.00 193.00 183.00 158.00 170.00 167.00 191.00 197.00 213.00 194.00 190.00 206.00 171.00 181.00 195.00 178.00 192.00 199.00 182.00 195.00 208.00 202.00 213.00 217.00 206.00 208.00 206.00 195.00 160.00 167.00 170.00 212.00 220.00 219.00 SPILLED 196.00 199.00 182.00 184.00 195.00 166.00 203.00 192.00 198.00 183.00 192.00 179.00 193.00 199.00 181.00 205.00 197.00 197.00 179.00 184.00 150.00 167.00 162.00 211.00 195.00 219.00 185.00 193.00 204.00 174.00 186.00 187.00 164.00 202.00 190.00 179.00 Covance 6329-183 3M T-6295 PAGE: 28 WEEK 28 WEEK 32 175.00 177.00 173.00 196.00 202.00 194.00 197.00 189.00 192.00 181.00 178.00 170.00 164.00 173.00 200.00 193.00 207.00 172.00 189.00 SPILLED 178.00 181.00 185.00 170.00 197.00 202.00 192.00 168.00 174.00 188.00 194.00 202.00 193.00 197.00 188.00 186.00 181.00 186.00 167.00 164.00 174.00 184.00 203.00 196.00 170.00 183.00 182.00 174.00 177.00 177.00 159.00 193.00 182.00 180.00 1373 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 11 WEEK 12 WEEK 13 WEEK 14 WEEK 15 WEEK 16 WEEK 20 WEEK 24 GROUP: C92599 C92600 C92601 C92602 C92603 C92604 C92605 C92606 C92607 C92608 C92609 C92611 C92612 C92614 C92615 C92616 C92617 C92619 C92620 C92621 C92622 C92623 C92624 C92625 C92626 C92627 C92628 C92629 C92630 MALE 2 - 0. 5 PPM 216.00 177.00 160.00 170.00 197.00 187.00 197.00 196.00 207.00 SPILLED 176.00 170.00 185.00 SPILLED 190.00 175.00 208.00 177.00 153.00 SPILLED 186.00 159.00 182.00 217.00 197.00 SPILLED 214.00 SPILLED 237.00 211.00 170.00 164.00 177.00 200.00 188.00 199.00 197.00 203.00 227.00 173.00 173.00 178.00 SPILLED 193.00 168.00 218.00 172.00 162.00 211.00 185.00 163.00 169.00 213.00 192.00 SPILLED SPILLED 211.00 229.00 213.00 172.00 165.00 172.00 184.00 187.00 189.00 186.00 208.00 212.00 171.00 162.00 172.00 SPILLED 188.00 174.00 201.00 175.00 185.00 204.00 172.00 156.00 178.00 202.00 186.00 SPILLED SPILLED 206.00 232.00 215.00 NOT TAKEN 162.00 173.00 190.00 188.00 195.00 183.00 213.00 199.00 178.00 168.00 159.00 SPILLED 187.00 NOT TAKEN 208.00 178.00 175.00 NOT TAKEN 179.00 154.00 162.00 205.00 186.00 NOT TAKEN 222.00 191.00 230.00 210.00 166.00 174.00 178.00 189.00 194.00 182.00 205.00 125.00 180.00 171.00 184.00 SPILLED 191.00 213.00 182.00 164.00 179.00 161.00 179.00 215.00 180.00 SPILLED 210.00 235.00 210.00 176.00 168.00 195.00 196.00 202.00 195.00 208.00 170.00 184.00 164.00 184.00 SPILLED 190.00 209.00 171.00 170.00 191.00 172.00 184.00 214.00 152.00 212.00 217.00 239.00 216.00 178.00 178.00 198.00 187.00 207.00 212.00 217.00 193.00 167.00 189.00 SPILLED 189.00 213.00 173.00 167.00 183.00 168.00 180.00 202.00 188.00 221.00 230.00 SPILLED 208.00 180.00 165.00 193.00 198.00 187.00 208.00 208.00 193.00 168.00 122.00 SPILLED 196.00 212.00 159.00 160.00 190.00 160.00 178.00 205.00 195.00 211.00 201.00 231.00 Covance 6329-183 3M T-6295 PAGE: 29 WEEK 28 WEEK 32 215.00 178.00 173.00 195.00 191.00 173.00 180.00 201.00 198.00 154.00 175.00 SPILLED 187.00 207.00 160.00 160.00 179.00 158.00 183.00 195.00 164.00 213.00 213.00 SPILLED 225.00 176.00 165.00 196.00 185.00 180.00 199.00 201.00 194.00 165.00 183.00 SPILLED 182.00 201.00 163.00 162.00 177.00 153.00 187.00 185.00 SPILLED SPILLED 207.00 SPILLED 1374 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 11 WEEK 12 WEEK 13 WEEK 14 WEEK 15 WEEK 16 WEEK 20 WEEK 24 GROUP: MALE 3 - 2 PPM C92631 C92632 C92633 C92634 C92635 C92636 C92637 C92638 C92639 C92640 C92641 C92642 C92643 C92644 C92645 C92647 C92648 C92649 C92651 C92653 C92654 C92655 C92656 C92657 C92658 C92659 C92660 C92661 C92662 C92663 C92664 178.00 203.00 173.00 184.00 SPILLED SPILLED 153.00 229.00 177.00 158.00 135.00 182.00 173.00 172.00 194.00 167.00 153.00 170.00 197.00 185.00 188.00 SPILLED 181.00 SPILLED 189.00 197.00 179.00 161.00 164.00 205.00 195.00 182.00 199.00 176.00 183.00 206.00 193.00 159.00 SPILLED 179.00 161.00 169.00 184.00 171.00 173.00 204.00 172.00 159.00 178.00 205.00 188.00 200.00 SPILLED 191.00 SPILLED 198.00 207.00 172.00 164.00 163.00 210.00 196.00 179.00 195.00 173.00 186.00 194.00 202.00 157.00 216.00 181.00 160.00 190.00 191.00 174.00 168.00 208.00 172.00 157.00 182.00 196.00 190.00 192.00 SPILLED 194.00 190.00 197.00 204.00 171.00 169.00 172.00 216.00 192.00 177.00 197.00 173.00 200.00 194.00 179.00 160.00 229.00 182.00 NOT TAKEN 199.00 179.00 176.00 154.00 NOT TAKEN 166.00 166.00 179.00 176.00 194.00 197.00 246.00 183.00 186.00 193.00 194.00 179.00 154.00 NOT TAKEN 217.00 195.00 177.00 238.00 171.00 205.00 189.00 SPILLED 164.00 214.00 182.00 199.00 184.00 168.00 180.00 177.00 170.00 182.00 198.00 187.00 196.00 SPILLED 190.00 192.00 196.00 218.00 173.00 189.00 212.00 194.00 188.00 205.00 182.00 183.00 191.00 206.00 163.00 230.00 189.00 212.00 186.00 SPILLED 178.00 180.00 170.00 179.00 202.00 183.00 190.00 SPILLED 190.00 187.00 189.00 208.00 176.00 187.00 214.00 200.00 182.00 209.00 193.00 201.00 191.00 202.00 169.00 227.00 183.00 206.00 175.00 169.00 172.00 176.00 174.00 190.00 189.00 199.00 180.00 237.00 186.00 201.00 201.00 209.00 203.00 178.00 214.00 209.00 180.00 220.00 186.00 198.00 201.00 189.00 165.00 244.00 198.00 206.00 174.00 179.00 168.00 176.00 167.00 172.00 202.00 186.00 190.00 239.00 180.00 190.00 180.00 195.00 198.00 180.00 224.00 195.00 Covance 6329-183 3M T-6295 PAGE: 30 WEEK 28 WEEK 32 181.00 202.00 182.00 182.00 189.00 187.00 159.00 227.00 186.00 165.00 179.00 171.00 164.00 169.00 165.00 169.00 191.00 189.00 182.00 238.00 178.00 179.00 184.00 200.00 177.00 177.00 210.00 179.00 169.00 196.00 177.00 177.00 190.00 191.00 162.00 228.00 173.00 176.00 173.00 169.00 177.00 175.00 161.00 171.00 193.00 193.00 182.00 229.00 175.00 182.00 186.00 198.00 SPILLED 166.00 208.00 193.00 1375 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 11 WEEK 12 WEEK 13 WEEK 14 WEEK 15 WEEK 16 WEEK 20 WEEK 24 GROUP: MALE 3 - 2 PPM C92665 C92666 C92667 C92669 C92670 C92671 C92672 C92673 C92674 C92675 C92676 C92677 C92679 C92680 C92681 C92682 C92683 C92684 C92685 C92686 C92687 C92688 C92689 C92690 198.00 212.00 210.00 SPILLED 171.00 189.00 174.00 182.00 SPILLED 189.00 175.00 166.00 194.00 204.00 180.00 175.00 189.00 182.00 189.00 172.00 203.00 SPILLED 168.00 211.00 194.00 224.00 211.00 207.00 175.00 184.00 179.00 188.00 191.00 180.00 166.00 162.00 189.00 217.00 183.00 174.00 189.00 102.00 178.00 173.00 203.00 201.00 152.00 210.00 GROUP: MALE 4 - 5 PPM C92691 C92692 C92693 C92694 178.00 174.00 192.00 176.00 173.00 177.00 184.00 170.00 206.00 220.00 217.00 206.00 178.00 186.00 170.00 192.00 195.00 181.00 168.00 165.00 195.00 209.00 174.00 179.00 189.00 167.00 184.00 178.00 206.00 201.00 160.00 215.00 200.00 215.00 214.00 SPILLED 172.00 183.00 180.00 186.00 203.00 180.00 NOT TAKEN 169.00 191.00 203.00 179.00 158.00 190.00 NOT TAKEN 194.00 184.00 204.00 SPILLED 168.00 216.00 203.00 212.00 197.00 218.00 173.00 189.00 183.00 184.00 209.00 180.00 175.00 200.00 205.00 180.00 181.00 190.00 194.00 175.00 208.00 SPILLED 178.00 215.00 171.00 180.00 178.00 155.00 171.00 182.00 186.00 172.00 186.00 177.00 193.00 181.00 206.00 228.00 206.00 200.00 171.00 191.00 182.00 186.00 198.00 190.00 180.00 202.00 193.00 193.00 177.00 181.00 191.00 181.00 196.00 198.00 176.00 206.00 195.00 225.00 215.00 194.00 175.00 188.00 169.00 187.00 201.00 188.00 189.00 205.00 200.00 181.00 190.00 199.00 195.00 189.00 201.00 SPILLED 175.00 224.00 196.00 248.00 199.00 191.00 163.00 182.00 180.00 175.00 207.00 187.00 181.00 198.00 190.00 187.00 177.00 200.00 189.00 186.00 202.00 SPILLED 180.00 198.00 186.00 175.00 189.00 189.00 192.00 169.00 179.00 189.00 184.00 161.00 177.00 168.00 Covance 6329-183 3M T-6295 PAGE: 31 WEEK 28 WEEK 32 193.00 236.00 202.00 186.00 164.00 179.00 166.00 164.00 182.00 178.00 182.00 202.00 187.00 SPILLED 173.00 191.00 177.00 183.00 199.00 SPILLED 166.00 203.00 197.0 0 SPILLEDi 198.0 0 187.0 0 158.0 0 184.0 0 172.0 0 174.0 0 186.0 0 188.0 0 181.0 0 202.0 0 181.0 0 174.0 0 179.0 0 195.0 0 188.0 0 173.0 0 193.0 0 198.0 0 161.0 0 204.0 0 178.00 167.00 171.00 174.00 178.0 0 161.0 0 178.0 0 176.0 0 1376 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 11 WEEK 12 WEEK 13 WEEK 14 WEEK 15 WEEK 16 WEEK 20 WEEK 24 GROUP: MALE 4 - 5 PPM C92695 C92696 C92697 C92698 C92699 C92700 C92701 C92702 C92703 C92704 C92705 C92706 C92707 C92708 C92709 C92710 C92711 C92712 C92713 C92714 C92716 C92717 C92719 C92720 C92721 C92722 C92723 C92724 C92725 C92726 C92727 182.00 196.00 213.00 192.00 166.00 150.00 174.00 166.00 214.00 189.00 192.00 172.00 160.00 174.00 177.00 194.00 169.00 202.00 197.00 208.00 193.00 187.00 180.00 SPILLED 161.00 203.00 SPILLED SPILLED 170.00 187.00 180.00 183.00 SPILLED 212.00 192.00 168.00 150.00 177.00 169.00 222.00 186.00 191.00 179.00 154.00 159.00 176.00 197.00 170.00 198.00 200.00 203.00 199.00 196.00 181.00 SPILLED 164.00 208.00 SPILLED SPILLED 164.00 182.00 194.00 177.00 192.00 212.00 197.00 165.00 153.00 179.00 163.00 215.00 181.00 184.00 175.00 154.00 178.00 177.00 186.00 172.00 204.00 193.00 206.00 197.00 189.00 184.00 SPILLED 168.00 202.00 SPILLED 248.00 164.00 181.00 194.00 178.00 182.00 SPILLED 198.00 167.00 151.00 179.00 165.00 216.00 189.00 192.00 176.00 154.00 175.00 172.00 188.00 146.00 222.00 NOT TAKEN NOT TAKEN 171.00 195.00 NOT TAKEN SPILLED 163.00 NOT TAKEN SPILLED SPILLED 167.00 160.00 174.00 180.00 188.00 199.00 200.00 166.00 151.00 174.00 167.00 210.00 185.00 198.00 181.00 159.00 182.00 184.00 191.00 177.00 224.00 SPILLED 198.00 SPILLED 170.00 SPILLED SPILLED 163.00 203.00 181.00 185.00 189.00 212.00 189.00 169.00 157.00 175.00 167.00 222.00 191.00 190.00 172.00 160.00 180.00 180.00 187.00 183.00 215.00 203.00 208.00 239.00 180.00 215.00 255.00 165.00 190.00 195.00 180.00 191.00 216.00 202.00 121.00 162.00 186.00 164.00 219.00 187.00 197.00 171.00 164.00 173.00 178.00 184.00 176.00 SPILLED 204.00 204.00 SPILLED 164.00 SPILLED 235.00 167.00 173.00 196.00 174.00 187.00 206.00 184.00 164.00 143.00 169.00 159.00 204.00 175.00 188.00 169.00 154.00 161.00 173.00 174.00 170.00 220.00 193.00 187.00 227.00 167.00 223.00 232.00 154.00 188.00 193.00 Covance 6329-183 3M T-6295 PAGE: 32 WEEK 28 WEEK 32 179.00 SPILLED 190.00 176.00 150.00 159.00 176.00 159.00 195.00 179.00 189.00 181.00 155.00 159.00 171.00 179.00 168.00 219.00 189.00 190.00 224.00 171.00 SPILLED SPILLED 151.00 181.00 178.00 170.0 0 155.0 0 196.0 0 183.0 0 173.0 0 SPILLEDi 172.0 0 169.0 0 189.0 0 171.0 0 178.0 0 188.0 0 163.0 0 160.0 0 160.0 0 167.0 0 170.0 0 215.0 0 195.0 0 194.0 0 216.0 0 162.0 0 SPILLEDi 216.0 0 153.0 0 170.0 0 189.0 0 1377 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 11 WEEK 12 WEEK 13 WEEK 14 WEEK 15 WEEK 16 WEEK 20 WEEK 24 GROUP: C92728 C92729 C92730 C92732 C92733 C92735 C92736 C92737 C92738 C92739 C92740 C92741 C92742 C92743 C92745 C92746 C92747 C92748 C92749 C92750 MALE 4 - 5 PPM 219.00 210.00 SPILLED 190.00 176.00 181.00 170.00 181.00 184.00 SPILLED 188.00 181.00 208.00 SPILLED 203.00 SPILLED 180.00 SPILLED SPILLED SPILLED 220.00 216.00 177.00 201.00 171.00 183.00 174.00 177.00 183.00 182.00 187.00 178.00 207.00 203.00 205.00 SPILLED 187.00 SPILLED 175.00 185.00 GROUP: MALE 5 - 20 PPM C92751 C92753 C92754 C92755 C92756 C92757 C92758 C92760 177.00 154.00 153.00 170.00 224.00 169.00 161.00 184.00 164.00 SPILLED 166.00 170.00 244.00 165.00 162.00 199.00 215.00 224.00 173.00 197.00 174.00 183.00 176.00 173.00 176.00 174.00 185.00 182.00 208.00 208.00 204.00 184.00 182.00 SPILLED 156.00 182.00 185.00 202.00 158.00 171.00 237.00 161.00 164.00 187.00 NOT TAKEN 216.00 179.00 195.00 187.00 187.00 176.00 181.00 181.00 171.00 186.00 167.00 203.00 213.00 211.00 181.00 189.00 SPILLED 175.00 185.00 207.00 185.00 198.00 192.00 187.00 181.00 186.00 187.00 174.00 184.00 188.00 206.00 213.00 210.00 194.00 190.00 SPILLED 182.00 189.00 184.00 SPILLED 142.00 175.00 SPILLED 163.00 162.00 188.00 196.00 SPILLED 159.00 176.00 SPILLED 165.00 166.00 181.00 212.00 186.00 206.00 178.00 199.00 175.00 182.00 170.00 181.00 185.00 183.00 203.00 212.00 220.00 192.00 185.00 SPILLED 179.00 182.00 199.00 218.00 154.00 179.00 239.00 176.00 167.00 179.00 217.00 174.00 197.00 202.00 179.00 185.00 185.00 173.00 184.00 185.00 204.00 189.00 220.00 214.00 198.00 182.00 238.00 181.00 183.00 196.00 SPILLED 162.00 180.00 SPILLED 182.00 175.00 189.00 212.00 183.00 182.00 191.00 172.00 182.00 174.00 183.00 188.00 171.00 186.00 188.00 196.00 213.00 190.00 177.00 229.00 168.00 177.00 190.00 199.00 149.00 173.00 245.00 188.00 157.00 176.00 Covance 6329-183 3M T-6295 PAGE: 33 WEEK 28 WEEK 32 209.00 185.00 189.00 198.00 174.00 178.00 179.00 171.00 173.00 168.00 SPILLED 188.00 197.00 199.00 189.00 181.00 214.00 179.00 179.00 208.0 0 176.0 0 184.0 0 181.0 0 183.0 0 175.0 0 180.0 0 172.0 0 174.0 0 179.0 0 SPILLEDi 194.0 0 208.0 0 229.0 0 191.0 0 176.0 0 209.0 0 170.0 0 177.0 0 187.00 159.00 152.00 168.00 SPILLED 173.00 154.00 185.00 188.0 0 177.0 0 157.0 0 172.0 0 215.0 0 175.0 0 155.0 0 176.0 0 1378 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 11 WEEK 12 WEEK 13 WEEK 14 WEEK 15 WEEK 16 WEEK 20 WEEK 24 GROUP: C92761 C92762 C92763 C92764 C92765 C92766 C92767 C92768 C92769 C92770 C92771 C92772 C92773 C92774 C92775 C92776 C92777 C92778 C92780 C92781 C92782 C92783 C92784 C92785 C92786 C92787 C92788 C92789 C92790 C92791 C92792 MALE 5 - 2 0 PPM 171.00 198.00 196.00 220.00 188.00 170.00 225.00 190.00 193.00 191.00 171.00 171.00 158.00 180.00 202.00 182.00 171.00 183.00 SPILLED 166.00 174.00 186.00 183.00 183.00 177.00 SPILLED 192.00 SPILLED 180.00 169.00 179.00 167.00 195.00 208.00 234.00 181.00 174.00 227.00 188.00 199.00 195.00 SPILLED 187.00 163.00 190.00 207.00 187.00 177.00 191.00 174.00 158.00 174.00 188.00 190.00 175.00 178.00 199.00 183.00 173.00 179.00 169.00 183.00 172.00 177.00 198.00 215.00 187.00 171.00 223.00 184.00 194.00 180.00 166.00 183.00 152.00 195.00 202.00 191.00 173.00 189.00 187.00 170.00 173.00 187.00 179.00 180.00 168.00 SPILLED 188.00 147.00 182.00 170.00 180.00 174.00 176.00 206.00 221.00 NOT TAKEN 175.00 188.00 184.00 197.00 180.00 181.00 181.00 153.00 172.00 205.00 190.00 NOT TAKEN 187.00 187.00 175.00 179.00 189.00 165.00 174.00 169.00 SPILLED 200.00 NOT TAKEN 185.00 173.00 177.00 164.00 177.00 206.00 212.00 183.00 224.00 191.00 201.00 183.00 178.00 187.00 159.00 203.00 199.00 190.00 192.00 181.00 173.00 182.00 187.00 187.00 178.00 181.00 SPILLED 198.00 179.00 176.00 183.00 177.00 175.00 204.00 225.00 176.00 SPILLED 186.00 199.00 182.00 167.00 188.00 161.00 192.00 204.00 177.00 190.00 178.00 161.00 179.00 195.00 189.00 180.00 186.00 198.00 189.00 166.00 182.00 190.00 186.00 191.00 208.00 211.00 189.00 239.00 196.00 220.00 184.00 188.00 187.00 160.00 190.00 212.00 189.00 185.00 185.00 162.00 172.00 192.00 176.00 175.00 188.00 199.00 194.00 175.00 189.00 185.00 179.00 171.00 206.00 213.00 188.00 238.00 179.00 200.00 173.00 184.00 181.00 153.00 186.00 198.00 173.00 172.00 182.00 168.00 172.00 190.00 172.00 162.00 178.00 196.00 178.00 168.00 178.00 180.00 Covance 6329-183 3M T-6295 PAGE: 34 WEEK 28 WEEK 32 175.00 178.00 217.00 217.00 177.00 235.00 184.00 188.00 189.00 184.00 189.00 167.00 161.00 199.00 178.00 173.00 194.00 160.00 166.00 199.00 183.00 167.00 183.00 215.00 188.00 171.00 185.00 169.00 188.00 181.00 211.00 213.00 175.00 242.00 SPILLED 199.00 187.00 176.00 185.00 163.00 175.00 201.00 178.00 180.00 188.00 167.00 165.00 193.00 185.00 164.00 182.00 218.00 180.00 171.00 183.00 178.00 1379 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 11 WEEK 12 WEEK 13 WEEK 14 WEEK 15 WEEK 16 WEEK 20 WEEK 24 GROUP: MALE 5 - 20 PPM C92794 C92795 C92796 C92797 C92799 C92800 C92801 C92802 C92803 C92804 C92805 C92806 C92807 C92808 C92809 C92810 C92811 C92812 C92813 C92814 C92815 C92816 C92817 C92818 C92819 C92820 164.00 178.00 150.00 165.00 191.00 SPILLED 197.00 176.00 178.00 178.00 193.00 166.00 176.00 155.00 190.00 177.00 197.00 167.00 190.00 130.00 SPILLED 152.00 197.00 174.00 152.00 SPILLED 160.00 174.00 148.00 171.00 190.00 173.00 195.00 174.00 176.00 175.00 191.00 178.00 172.00 147.00 183.00 176.00 TECH SPILL 169.00 174.00 129.00 SPILLED 148.00 195.00 167.00 144.00 232.00 165.00 164.00 147.00 167.00 202.00 174.00 192.00 176.00 178.00 182.00 194.00 181.00 170.00 153.00 198.00 179.00 187.00 169.00 183.00 125.00 SPILLED 150.00 199.00 169.00 150.00 227.00 GROUP: MALE 6 - 20 PPM C92821 C92822 172.00 162.00 186.00 163.00 182.00 160.00 161.00 173.00 151.00 174.00 NOT TAKEN 171.00 191.00 175.00 179.00 188.00 190.00 167.00 173.00 154.00 196.00 181.00 197.00 NOT TAKEN 191.00 135.00 195.00 154.00 199.00 172.00 162.00 176.00 161.00 181.00 159.00 175.00 171.00 205.00 181.00 182.00 182.00 192.00 163.00 189.00 159.00 201.00 188.00 205.00 185.00 135.00 SPILLED 152.00 201.00 176.00 157.00 214.00 173.00 174.00 174.00 173.00 159.00 187.00 161.00 180.00 171.00 196.00 185.00 185.00 185.00 185.00 176.00 184.00 160.00 195.00 176.00 210.00 184.00 145.00 200.00 149.00 201.00 180.00 162.00 199.00 155.00 182.00 151.00 197.00 172.00 188.00 180.00 185.00 187.00 192.00 171.00 173.00 157.00 195.00 171.00 209.00 182.00 132.00 SPILLED 148.00 213.00 180.00 167.00 177.00 161.00 171.00 158.00 187.00 158.00 203.00 175.00 182.00 179.00 181.00 168.00 166.00 150.00 168.00 172.00 200.00 180.00 125.00 185.00 145.00 202.00 166.00 151.00 155.00 162.00 166.00 170.00 183.00 SPILLED 163.00 Covance 6329-183 3M T-6295 PAGE: 35 WEEK 28 WEEK 32 149.00 175.00 157.00 183.00 165.00 208.00 185.00 180.00 175.00 181.00 187.00 167.00 158.00 177.00 173.00 202.00 184.00 140.00 199.00 157.00 211.00 165.00 158.00 194.00 203.00 176.00 159.00 175.00 159.00 188.00 159.00 204.00 193.00 193.00 186.00 178.00 178.00 171.00 154.00 170.00 176.00 198.00 199.00 136.00 195.00 146.00 222.00 177.00 165.00 200.00 178.00 169.00 1380 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 11 WEEK 12 WEEK 13 WEEK 14 WEEK 15 WEEK 16 WEEK 20 WEEK 24 GROUP: C92823 C92824 C92825 C92826 C92827 C92828 C92829 C92830 C92831 C92832 C92833 C92834 C92835 C92836 C92837 C92838 C92839 C92840 C92841 C92842 C92843 C92844 C92845 C92846 C92847 C92848 C92849 C92850 C92851 C92852 C92853 MALE 6 - 2 0 PPM 175.00 182.00 243.00 174.00 177.00 180.00 196.00 182.00 189.00 SPILLED 179.00 176.00 160.00 198.00 182.00 174.00 232.00 153.00 167.00 189.00 195.00 143.00 194.00 194.00 213.00 170.00 SPILLED 181.00 168.00 191.00 164.00 174.00 182.00 211.00 202.00 178.00 176.00 201.00 186.00 195.00 161.00 177.00 174.00 157.00 179.00 178.00 174.00 236.00 152.00 156.00 180.00 185.00 132.00 186.00 194.00 213.00 169.00 SPILLED 178.00 170.00 180.00 159.00 170.00 182.00 215.00 188.00 188.00 181.00 203.00 192.00 192.00 163.00 181.00 165.00 156.00 175.00 177.00 181.00 242.00 160.00 120.00 184.00 183.00 136.00 SPILLED 194.00 212.00 180.00 SPILLED SPILLED 169.00 173.00 159.00 174.00 191.00 223.00 195.00 205.00 191.00 208.00 194.00 164.00 170.00 191.00 175.00 162.00 189.00 175.00 189.00 236.00 158.00 169.00 183.00 175.00 140.00 196.00 180.00 210.00 SPILLED SPILLED 185.00 176.00 165.00 165.00 181.00 187.00 224.00 189.00 199.00 187.00 210.00 194.00 197.00 164.00 188.00 177.00 171.00 190.00 184.00 180.00 248.00 162.00 171.00 166.00 186.00 144.00 188.00 186.00 216.00 172.00 214.00 181.00 188.00 170.00 160.00 187.00 193.00 SPILLED 188.00 195.00 180.00 206.00 187.00 200.00 153.00 186.00 178.00 169.00 177.00 171.00 177.00 248.00 161.00 161.00 184.00 200.00 151.00 190.00 196.00 206.00 166.00 221.00 SPILLED 188.00 180.00 170.00 154.00 189.00 243.00 205.00 190.00 194.00 208.00 144.00 192.00 162.00 185.00 185.00 172.00 180.00 195.00 178.00 237.00 153.00 160.00 187.00 209.00 158.00 195.00 192.00 211.00 172.00 SPILLED SPILLED 178.00 169.00 181.00 126.00 180.00 227.00 193.00 187.00 185.00 195.00 170.00 190.00 156.00 181.00 183.00 163.00 167.00 180.00 174.00 238.00 155.00 147.00 181.00 175.00 147.00 182.00 176.00 203.00 177.00 225.00 180.00 173.00 158.00 169.00 Covance 6329-183 3M T-6295 PAGE: 36 WEEK 28 WEEK 32 174.00 SPILLED 230.00 192.00 180.00 182.00 199.00 174.00 176.00 151.00 177.00 178.00 166.00 176.00 184.00 180.00 231.00 159.00 152.00 173.00 188.00 151.00 182.00 SPILLED 197.00 169.00 SPILLED SPILLED 184.00 165.00 172.00 164.00 SPILLED SPILLED 187.00 180.00 181.00 202.00 177.00 170.00 159.00 182.00 181.00 166.00 178.00 197.00 181.00 240.00 160.00 146.00 182.00 179.00 141.00 183.00 207.00 200.00 164.00 SPILLED SPILLED 210.00 186.00 178.00 1381 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 11 WEEK 12 WEEK 13 WEEK 14 WEEK 15 WEEK 16 WEEK 20 WEEK 24 GROUP: C92854 C92855 C92856 C92857 C92858 C92859 C92860 GROUP: C92862 C92863 C92865 C92866 C92867 C92868 C92869 C92870 C92871 C92872 C92873 C92874 C92875 C92876 C92877 C92878 C92879 C92880 C92881 C92882 C92883 MALE 6 - 20 PPM 186.00 183.00 167.00 172.00 191.00 180.00 173.00 185.00 171.00 160.00 168.00 186.00 179.00 167.00 FEMALE 1 - 0 PPM 119.00 119.00 128.00 118.00 158.00 154.00 SPILLED 128.00 138.00 109.00 133.00 126.00 132.00 112.00 SPILLED SPILLED 148.00 133.00 151.00 103.00 154.00 122.00 130.00 154.00 126.00 150.00 149.00 SPILLED 132.00 SPILLED 102.00 133.00 126.00 142.00 110.00 SPILLED SPILLED 135.00 148.00 169.00 107.00 164.00 181.00 179.00 169.00 171.00 189.00 176.00 166.00 125.00 122.00 137.00 121.00 128.00 151.00 SPILLED 129.00 143.00 102.00 125.00 118.00 125.00 114.00 142.00 SPILLED 134.00 147.00 SPILLED 108.00 139.00 184.00 247.00 171.00 178.00 188.00 182.00 175.00 187.00 195.00 174.00 179.00 192.00 184.00 172.00 128.00 99.00 132.00 116.00 114.00 139.00 SPILLED 130.00 125.00 105.00 135.00 122.00 140.00 115.00 SPILLED SPILLED 162.00 NOT TAKEN 140.00 106.00 156.00 129.00 115.00 151.00 122.00 130.00 143.00 SPILLED 142.00 SPILLED 102.00 134.00 122.00 140.00 125.00 144.00 SPILLED 140.00 140.00 110.00 140.00 186.00 183.00 171.00 183.00 186.00 177.00 171.00 121.00 117.00 139.00 121.00 132.00 118.00 SPILLED 131.00 144.00 107.00 123.00 128.00 146.00 105.00 157.00 SPILLED 135.00 130.00 112.00 134.00 195.00 153.00 166.00 181.00 193.00 190.00 179.00 105.00 123.00 165.00 125.00 132.00 154.00 SPILLED 137.00 SPILLED 155.00 145.00 126.00 SPILLED 130.00 143.00 SPILLED 141.00 107.00 127.00 151.00 189.00 169.00 164.00 163.00 193.00 192.00 174.00 118.00 117.00 151.00 121.00 139.00 134.00 SPILLED 137.00 SPILLED SPILLED 114.00 115.00 136.00 125.00 147.00 SPILLED 140.00 156.00 94.00 139.00 Covance 6329-183 3M T-6295 PAGE: 37 WEEK 28 WEEK 32 197.00 179.00 166.00 167.00 188.00 185.00 168.00 183.00 167.00 168.00 164.00 SPILLED 187.00 163.00 119.00 110.00 142.00 127.00 151.00 118.00 SPILLED 138.00 141.00 SPILLED 122.00 117.00 148.00 134.00 123.00 SPILLED 135.00 143.00 116.00 125.00 124.00 145.00 151.00 128.00 167.00 127.00 SPILLED 146.00 129.00 154.00 114.00 133.00 142.00 114.00 126.00 SPILLED 145.00 139.00 115.00 130.00 1382 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 11 WEEK 12 WEEK 13 WEEK 14 WEEK 15 WEEK 16 WEEK 20 WEEK 24 GROUP: C92884 C92885 C92886 C92887 C92888 C92889 C92890 C92891 C92892 C92893 C92894 C92895 C92896 C92897 C92898 C92899 C92900 C92901 C92902 C92903 C92904 C92905 C92906 C92907 C92908 C92909 C92910 C92911 C92912 C92913 C92915 FEMALE 1 - 0 PPM SPILLED 144.00 119.00 132.00 156.00 121.00 140.00 SPILLED 145.00 107.00 131.00 154.00 SPILLED 125.00 137.00 178.00 143.00 124.00 164.00 169.00 121.00 130.00 111.00 123.00 123.00 130.00 SPILLED 114.00 138.00 129.00 153.00 143.00 141.00 128.00 133.00 159.00 113.00 SPILLED SPILLED 153.00 121.00 135.00 133.00 SPILLED 135.00 142.00 177.00 108.00 115.00 162.00 168.00 SPILLED 129.00 SPILLED 118.00 131.00 126.00 SPILLED 117.00 135.00 121.00 148.00 128.00 143.00 122.00 138.00 157.00 104.00 142.00 SPILLED 146.00 119.00 135.00 145.00 143.00 127.00 134.00 152.00 117.00 111.00 172.00 165.00 119.00 127.00 107.00 115.00 134.00 130.00 SPILLED 113.00 125.00 116.00 SPILLED 129.00 135.00 120.00 NOT TAKEN 147.00 103.00 136.00 137.00 130.00 108.00 134.00 133.00 132.00 125.00 NOT TAKEN 147.00 SPILLED 105.00 183.00 NOT TAKEN 117.00 NOT TAKEN 100.00 122.00 116.00 125.00 SPILLED 109.00 132.00 117.00 151.00 135.00 145.00 120.00 149.00 112.00 136.00 141.00 131.00 107.00 125.00 142.00 130.00 123.00 168.00 SPILLED 115.00 163.00 116.00 116.00 111.00 119.00 128.00 SPILLED 117.00 144.00 124.00 147.00 138.00 137.00 127.00 158.00 113.00 152.00 131.00 135.00 111.00 116.00 125.00 132.00 124.00 171.00 120.00 119.00 149.00 106.00 SPILLED 119.00 126.00 121.00 SPILLED 117.00 SPILLED 115.00 132.00 159.00 141.00 136.00 165.00 129.00 SPILLED SPILLED 150.00 106.00 137.00 SPILLED 143.00 131.00 SPILLED 143.00 118.00 165.00 126.00 133.00 117.00 119.00 124.00 SPILLED 123.00 144.00 116.00 133.00 174.00 127.00 121.00 153.00 116.00 130.00 124.00 160.00 111.00 137.00 123.00 146.00 127.00 175.00 130.00 115.00 158.00 114.00 SPILLED 121.00 133.00 112.00 SPILLED 109.00 157.00 112.00 135.00 Covance 6329-183 3M T-6295 PAGE: 38 WEEK 28 WEEK 32 148.00 131.00 137.00 142.00 112.00 130.00 126.00 137.00 117.00 128.00 122.00 133.00 139.00 169.00 116.00 114.00 149.00 124.00 110.00 114.00 126.00 115.00 SPILLED 123.00 122.00 114.00 156.00 132.00 127.00 133.00 143.00 106.00 125.00 133.00 149.00 117.00 123.00 118.00 109.00 144.00 171.00 128.00 118.00 173.00 138.00 130.00 115.00 112.00 114.00 120.00 121.00 163.00 114.00 155.00 1383 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 11 WEEK 12 WEEK 13 WEEK 14 WEEK 15 WEEK 16 WEEK 20 WEEK 24 GROUP: FEMALE 1 - 0 PPM C92916 C92917 C92918 C92920 C92921 C92922 C92923 C92924 C92925 C92927 C92928 C92929 C92930 169.00 174.00 123.00 205.00 SPILLED SPILLED 145.00 153.00 134.00 172.00 141.00 117.00 193.00 175.00 161.00 123.00 SPILLED 156.00 149.00 SPILLED SPILLED 137.00 111.00 142.00 119.00 195.00 GROUP: FEMALE 2 - 0.5 PPM C92931 C92932 C92933 C92934 C92935 C92936 C92937 C92938 C92939 C92940 C92942 C92943 C92944 C92946 C92947 141.00 SPILLED 165.00 150.00 127.00 108.00 133.00 138.00 118.00 SPILLED 145.00 131.00 178.00 109.00 144.00 130.00 SPILLED 162.00 SPILLED 124.00 120.00 145.00 SPILLED SPILLED SPILLED SPILLED 137.00 SPILLED 110.00 130.00 SPILLED 165.00 123.00 144.00 146.00 135.00 127.00 SPILLED 139.00 SPILLED 134.00 121.00 197.00 139.00 SPILLED 148.00 134.00 123.00 113.00 138.00 196.00 SPILLED SPILLED 151.00 144.00 SPILLED 115.00 136.00 183.00 162.00 132.00 SPILLED 151.00 136.00 122.00 SPILLED 129.00 132.00 138.00 115.00 195.00 SPILLED 166.00 125.00 144.00 SPILLED 132.00 124.00 151.00 129.00 155.00 144.00 118.00 190.00 129.00 SPILLED 134.00 SPILLED 101.00 108.00 138.00 SPILLED SPILLED SPILLED SPILLED 162.00 NOT TAKEN 99.00 98.00 143.00 SPILLED 142.00 130.00 117.00 114.00 140.00 146.00 154.00 176.00 156.00 153.00 109.00 125.00 192.00 158.00 121.00 177.00 131.00 126.00 123.00 SPILLED 128.00 157.00 148.00 120.00 186.00 144.00 146.00 155.00 131.00 120.00 113.00 139.00 SPILLED SPILLED 138.00 SPILLED 145.00 114.00 128.00 172.00 170.00 116.00 173.00 145.00 148.00 138.00 SPILLED 141.00 151.00 157.00 117.00 191.00 150.00 SPILLED 155.00 126.00 127.00 101.00 150.00 SPILLED SPILLED 130.00 147.00 130.00 94.00 133.00 148.00 162.00 111.00 148.00 153.00 146.00 123.00 137.00 121.00 SPILLED 136.00 126.00 183.00 150.00 173.00 156.00 127.00 123.00 98.00 144.00 SPILLED SPILLED 123.00 140.00 112.00 115.00 106.00 Covance 6329-183 3M T-6295 PAGE: 39 WEEK 28 WEEK 32 159.00 156.00 132.00 146.00 148.00 145.00 119.00 118.00 146.00 157.00 126.00 120.00 176.00 170.00 160.00 119.00 125.00 159.00 142.00 132.00 138.00 140.00 159.00 148.00 145.00 187.00 162.00 175.00 159.00 123.00 111.00 111.00 144.00 125.00 130.00 120.00 142.00 127.00 100.00 114.00 164.00 246.00 160.00 118.00 144.00 107.00 141.00 SPILLED SPILLED 120.00 142.00 151.00 117.00 115.00 1384 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 11 WEEK 12 WEEK 13 WEEK 14 WEEK 15 WEEK 16 WEEK 20 WEEK 24 GROUP: C92948 C92949 C92951 C92952 C92953 C92954 C92955 C92956 C92957 C92958 C92959 C92960 C92961 C92962 C92963 C92964 C92965 C92966 C92967 C92968 C92970 C92971 C92972 C92973 C92974 C92975 C92976 C92977 C92978 C92979 C92980 FEMALE 2 - 0.5 PPM 143.00 144.00 104.00 127.00 120.00 133.00 139.00 142.00 131.00 111.00 152.00 131.00 168.00 116.00 113.00 123.00 127.00 112.00 127.00 133.00 138.00 107.00 141.00 137.00 116.00 142.00 137.00 146.00 152.00 132.00 143.00 130.00 165.00 SPILLED 117.00 115.00 136.00 131.00 121.00 125.00 108.00 143.00 155.00 159.00 116.00 126.00 SPILLED 132.00 116.00 SPILLED 134.00 148.00 110.00 146.00 133.00 110.00 142.00 136.00 171.00 155.00 115.00 136.00 144.00 175.00 SPILLED 117.00 119.00 SPILLED 132.00 124.00 SPILLED 112.00 159.00 153.00 162.00 117.00 115.00 121.00 122.00 120.00 125.00 138.00 148.00 106.00 144.00 SPILLED 113.00 138.00 124.00 154.00 150.00 127.00 137.00 115.00 168.00 114.00 134.00 113.00 138.00 127.00 103.00 SPILLED 100.00 157.00 144.00 160.00 NOT TAKEN 125.00 SPILLED 120.00 115.00 NOT TAKEN 131.00 147.00 113.00 133.00 147.00 127.00 135.00 125.00 144.00 NOT TAKEN 125.00 133.00 138.00 163.00 115.00 125.00 108.00 131.00 131.00 129.00 134.00 110.00 139.00 149.00 164.00 115.00 129.00 125.00 111.00 133.00 152.00 101.00 145.00 146.00 123.00 135.00 130.00 138.00 122.00 135.00 133.00 175.00 120.00 124.00 117.00 132.00 132.00 133.00 131.00 119.00 151.00 144.00 158.00 116.00 207.00 125.00 121.00 133.00 153.00 107.00 158.00 145.00 121.00 144.00 137.00 SPILLED 117.00 130.00 146.00 166.00 126.00 132.00 122.00 125.00 136.00 137.00 131.00 124.00 156.00 149.00 149.00 119.00 SPILLED 125.00 124.00 132.00 135.00 105.00 154.00 137.00 116.00 148.00 135.00 133.00 105.00 137.00 149.00 155.00 SPILLED 131.00 109.00 116.00 147.00 126.00 SPILLED 113.00 154.00 SPILLED 151.00 107.00 SPILLED 132.00 109.00 131.00 135.00 96.00 140.00 167.00 107.00 130.00 143.00 133.00 123.00 142.00 Covance 6329-183 3M T-6295 PAGE: 40 WEEK 28 WEEK 32 133.00 137.00 SPILLED 128.00 107.00 123.00 148.00 132.00 129.00 108.00 222.00 182.00 149.00 117.00 140.00 CNTMD 105.00 138.00 134.00 113.00 138.00 138.00 100.00 137.00 134.00 126.00 118.00 112.00 131.00 96.00 109.00 121.00 121.00 126.00 141.00 135.00 122.00 118.00 141.00 149.00 150.00 127.00 164.00 141.00 96.00 114.00 131.00 102.00 141.00 157.00 99.00 127.00 134.00 134.00 109.00 155.00 1385 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 11 WEEK 12 WEEK 13 WEEK 14 WEEK 15 WEEK 16 WEEK 20 WEEK 24 GROUP: FEMALE 2 - 0.5 PPM C92981 C92983 C92984 C92985 C92986 C92987 C92988 C92989 C92990 127.00 141.00 136.00 136.00 128.00 121.00 139.00 SPILLED 146.00 SPILLED 136.00 133.00 136.00 120.00 125.00 123.00 SPILLED 143.00 GROUP: C92991 C92992 C92993 C92994 C92997 C92998 C92999 C93000 C93001 C93002 C93003 C93004 C93005 C93007 C93009 C93010 C93011 C93012 C93013 FEMALE 3 - 2 PPM 111.00 129.00 131.00 122.00 119.00 146.00 SPILLED 129.00 128.00 126.00 115.00 126.00 119.00 123.00 SPILLED 148.00 130.00 147.00 117.00 110.00 120.00 130.00 130.00 114.00 146.00 SPILLED 149.00 135.00 131.00 132.00 130.00 121.00 SPILLED 168.00 146.00 122.00 136.00 128.00 128.00 141.00 132.00 150.00 126.00 124.00 136.00 SPILLED 134.00 108.00 119.00 125.00 117.00 113.00 145.00 142.00 126.00 125.00 124.00 132.00 125.00 130.00 111.00 129.00 142.00 117.00 138.00 110.00 129.00 SPILLED 123.00 133.00 109.00 NOT TAKEN 145.00 SPILLED 146.00 115.00 SPILLED NOT TAKEN 112.00 SPILLED 147.00 SPILLED NOT TAKEN 123.00 130.00 130.00 124.00 142.00 124.00 142.00 SPILLED 125.00 140.00 131.00 131.00 126.00 126.00 130.00 127.00 140.00 153.00 139.00 120.00 126.00 125.00 SPILLED 130.00 133.00 SPILLED 139.00 123.00 162.00 137.00 144.00 123.00 130.00 161.00 124.00 111.00 136.00 128.00 118.00 145.00 161.00 128.00 114.00 140.00 129.00 141.00 126.00 125.00 150.00 123.00 149.00 114.00 119.00 133.00 122.00 SPILLED 152.00 SPILLED 128.00 131.00 130.00 124.00 130.00 115.00 127.00 180.00 131.00 149.00 124.00 119.00 139.00 128.00 SPILLED 139.00 SPILLED 144.00 138.00 147.00 121.00 162.00 118.00 123.00 142.00 116.00 154.00 110.00 154.00 125.00 128.00 134.00 116.00 127.00 163.00 126.00 114.00 141.00 112.00 125.00 146.00 130.00 159.00 128.00 129.00 118.00 147.00 120.00 141.00 130.00 116.00 150.00 139.00 Covance 6329-183 3M T-6295 PAGE: 41 WEEK 28 WEEK 32 111.00 129.00 127.00 125.00 117.00 153.00 207.00 138.00 144.00 135.00 118.00 165.00 111.00 124.00 SPILLED 195.00 137.00 146.00 113.00 133.00 159.00 145.00 171.00 137.00 154.00 107.00 137.00 125.00 156.00 129.00 114.00 157.00 108.00 134.00 152.00 112.00 126.00 142.00 147.00 153.00 140.00 125.00 121.00 146.00 114.00 146.00 162.00 130.00 151.00 118.00 1386 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 11 WEEK 12 WEEK 13 WEEK 14 WEEK 15 WEEK 16 WEEK 20 WEEK 24 GROUP: C93014 C93015 C93016 C93017 C93018 C93019 C93020 C93021 C93022 C93023 C93024 C93025 C93026 C93027 C93028 C93029 C93030 C93032 C93033 C93034 C93035 C93036 C93037 C93038 C93039 C93040 C93041 C93042 C93043 C93044 C93046 FEMALE 3 - 2 PPM 135.00 134.00 99.00 144.00 136.00 140.00 131.00 135.00 134.00 SPILLED 139.00 166.00 139.00 122.00 133.00 SPILLED 136.00 123.00 SPILLED 149.00 145.00 SPILLED 129.00 SPILLED 152.00 SPILLED 165.00 137.00 142.00 125.00 116.00 155.00 SPILLED SPILLED 142.00 130.00 SPILLED 197.00 118.00 120.00 SPILLED 140.00 169.00 135.00 131.00 123.00 SPILLED SPILLED 131.00 SPILLED SPILLED 138.00 129.00 133.00 SPILLED 142.00 SPILLED SPILLED 138.00 149.00 115.00 169.00 139.00 SPILLED 118.00 146.00 128.00 SPILLED 137.00 124.00 122.00 SPILLED 131.00 167.00 123.00 126.00 SPILLED SPILLED 132.00 126.00 SPILLED 137.00 131.00 123.00 130.00 131.00 144.00 SPILLED 163.00 135.00 148.00 114.00 149.00 133.00 SPILLED 118.00 155.00 NOT TAKEN SPILLED 154.00 128.00 131.00 NOT TAKEN 130.00 166.00 109.00 157.00 SPILLED SPILLED 121.00 115.00 SPILLED 130.00 NOT TAKEN SPILLED 134.00 133.00 140.00 131.00 SPILLED 149.00 143.00 120.00 138.00 144.00 140.00 SPILLED 147.00 SPILLED 143.00 116.00 118.00 146.00 182.00 120.00 123.00 SPILLED SPILLED 144.00 124.00 SPILLED 126.00 136.00 134.00 134.00 132.00 SPILLED 146.00 129.00 157.00 120.00 142.00 131.00 SPILLED 146.00 145.00 147.00 136.00 128.00 125.00 135.00 134.00 121.00 125.00 134.00 SPILLED SPILLED 133.00 SPILLED 135.00 117.00 166.00 140.00 125.00 SPILLED 154.00 112.00 147.00 SPILLED 167.00 140.00 SPILLED SPILLED 140.00 158.00 145.00 149.00 119.00 127.00 174.00 130.00 120.00 SPILLED SPILLED 135.00 139.00 SPILLED 114.00 134.00 116.00 128.00 127.00 119.00 173.00 170.00 139.00 125.00 149.00 116.00 SPILLED SPILLED 128.00 SPILLED 161.00 138.00 118.00 129.00 136.00 130.00 124.00 130.00 SPILLED 136.00 135.00 194.00 129.00 144.00 107.00 128.00 126.00 144.00 180.00 158.00 156.00 SPILLED 148.00 Covance 6329-183 3M T-6295 PAGE: 42 WEEK 28 WEEK 32 141.00 132.00 193.00 146.00 149.00 152.00 167.00 145.00 155.00 144.00 134.00 117.00 155.00 119.00 134.00 138.00 132.00 168.00 129.00 119.00 SPILLED 171.00 124.00 137.00 SPILLED 131.00 126.00 140.00 134.00 119.00 SPILLED SPILLED 146.00 138.00 162.00 134.00 139.00 119.00 123.00 131.00 155.00 170.00 166.00 140.00 195.00 TECH SPILL 123.00 114.00 129.00 132.00 141.00 160.00 155.00 155.00 SPILLED SPILLED 1387 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 11 WEEK 12 WEEK 13 WEEK 14 WEEK 15 WEEK 16 WEEK 20 WEEK 24 GROUP: FEMALE 3 - 2 PPM C93047 C93048 C93049 C93050 155.00 128.00 124.00 141.00 152.00 SPILLED 121.00 SPILLED GROUP: FEMALE 4 - 5 PPM C93051 C93052 C93053 C93054 C93055 C93056 C93057 C93059 C93060 C93061 C93062 C93063 C93064 C93065 C93066 C93068 C93069 C93071 C93072 C93073 C93074 C93076 C93077 C93078 128.00 SPILLED 144.00 SPILLED SPILLED 126.00 130.00 136.00 141.00 137.00 128.00 122.00 132.00 118.00 137.00 129.00 144.00 115.00 160.00 143.00 180.00 SPILLED 135.00 135.00 143.00 SPILLED 132.00 SPILLED 191.00 125.00 129.00 136.00 140.00 116.00 121.00 112.00 122.00 131.00 126.00 131.00 143.00 112.00 159.00 141.00 SPILLED 132.00 127.00 170.00 142.00 133.00 121.00 153.00 129.00 SPILLED 129.00 132.00 137.00 128.00 134.00 129.00 130.00 125.00 121.00 127.00 121.00 116.00 127.00 124.00 146.00 110.00 SPILLED 144.00 156.00 SPILLED 125.00 SPILLED 149.00 126.00 123.00 SPILLED 157.00 125.00 121.00 150.00 NOT TAKEN SPILLED 143.00 142.00 SPILLED 125.00 134.00 133.00 134.00 123.00 120.00 119.00 NOT TAKEN 121.00 126.00 126.00 141.00 NOT TAKEN 139.00 137.00 SPILLED 108.00 119.00 SPILLED SPILLED 133.00 127.00 134.00 129.00 137.00 130.00 136.00 134.00 124.00 125.00 118.00 131.00 126.00 138.00 SPILLED 146.00 155.00 SPILLED 116.00 138.00 152.00 143.00 124.00 164.00 SPILLED 151.00 SPILLED 132.00 130.00 128.00 141.00 128.00 129.00 121.00 121.00 122.00 126.00 128.00 140.00 142.00 151.00 165.00 SPILLED 119.00 144.00 138.00 130.00 123.00 148.00 SPILLED 145.00 133.00 133.00 136.00 120.00 129.00 118.00 144.00 125.00 112.00 118.00 SPILLED 125.00 147.00 149.00 145.00 171.00 136.00 141.00 165.00 130.00 129.00 115.00 150.00 SPILLED 150.00 133.00 124.00 128.00 132.00 122.00 124.00 153.00 121.00 108.00 117.00 SPILLED 134.00 165.00 141.00 139.00 155.00 134.00 140.00 130.00 Covance 6329-183 3M T-6295 PAGE: 43 WEEK 28 WEEK 32 141.00 SPILLED 120.00 140.00 176.00 127.00 122.00 145.00 134.00 144.00 135.00 138.00 145.00 131.00 125.00 111.00 156.00 129.00 113.00 114.00 158.00 116.00 146.00 147.00 137.00 148.00 135.00 139.00 134.00 SPILLED 145.00 134.00 161.00 146.00 136.00 134.00 125.00 156.00 SPILLED 121.00 104.00 SPILLED 134.00 155.00 151.00 133.00 140.00 118.00 136.00 141.00 1388 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 11 WEEK 12 WEEK 13 WEEK 14 WEEK 15 WEEK 16 WEEK 20 WEEK 24 GROUP: C93079 C93080 C93081 C93082 C93083 C93085 C93086 C93087 C93088 C93089 C93090 C93091 C93092 C93093 C93094 C93095 C93096 C93097 C93098 C93099 C93100 C93101 C93102 C93103 C93104 C93105 C93106 C93107 C93108 C93109 C93110 FEMALE 4 - 5 PPM 144.00 128.00 116.00 121.00 135.00 132.00 127.90 122.00 108.00 147.00 123.00 126.00 132.00 133.00 132.00 123.00 149.00 SPILLED 120.00 123.00 156.00 119.00 SPILLED 137.00 SPILLED 137.00 122.00 125.00 120.00 133.00 199.00 151.00 129.00 120.00 121.00 134.00 133.00 155.00 126.00 132.00 135.00 113.00 130.00 130.00 134.00 135.00 SPILLED 159.00 SPILLED 131.00 114.00 149.00 111.00 SPILLED 130.00 211.00 143.00 113.00 117.00 SPILLED 129.00 200.00 151.00 125.00 125.00 123.00 138.00 SPILLED 129.00 107.00 125.00 127.00 117.00 123.00 115.00 160.00 123.00 135.00 154.00 SPILLED 117.00 123.00 156.00 98.00 147.00 139.00 192.00 134.00 110.00 110.00 114.00 115.00 212.00 145.00 119.00 115.00 121.00 137.00 NOT TAKEN SPILLED 111.00 132.00 115.00 105.00 130.00 120.00 113.00 122.00 126.00 SPILLED SPILLED 134.00 118.00 143.00 107.00 SPILLED 130.00 SPILLED 123.00 108.00 115.00 109.00 NOT TAKEN SPILLED 167.00 131.00 122.00 124.00 145.00 136.00 112.00 129.00 138.00 127.00 128.00 124.00 153.00 132.00 128.00 154.00 SPILLED 117.00 118.00 145.00 113.00 154.00 124.00 SPILLED 129.00 119.00 118.00 116.00 202.00 145.00 120.00 112.00 133.00 141.00 135.00 119.00 137.00 131.00 116.00 120.00 123.00 161.00 143.00 128.00 150.00 SPILLED 122.00 125.00 154.00 108.00 155.00 127.00 184.00 130.00 115.00 118.00 119.00 SPILLED 149.00 160.00 121.00 136.00 131.00 138.00 119.00 136.00 141.00 114.00 136.00 147.00 SPILLED 127.00 123.00 162.00 SPILLED 125.00 109.00 157.00 SPILLED 168.00 144.00 180.00 130.00 121.00 120.00 SPILLED 194.00 129.00 133.00 117.00 153.00 133.00 123.00 109.00 128.00 129.00 107.00 136.00 134.00 SPILLED 111.00 110.00 166.00 141.00 120.00 129.00 142.00 103.00 151.00 100.00 178.00 118.00 117.00 108.00 110.00 199.00 Covance 6329-183 3M T-6295 PAGE: 44 WEEK 28 WEEK 32 142.00 SPILLED 130.00 139.00 138.00 126.00 108.00 139.00 124.00 108.00 144.00 138.00 117.00 119.00 111.00 153.00 138.00 111.00 134.00 131.00 109.00 SPILLED 134.00 165.00 114.00 125.00 114.00 103.00 210.00 131.00 SPILLED 118.00 145.00 127.00 120.00 122.00 139.00 129.00 104.00 119.00 148.00 144.00 126.00 109.00 136.00 120.00 142.00 137.00 109.00 159.00 182.00 163.00 117.00 120.00 118.00 105.00 169.00 1389 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 11 WEEK 12 WEEK 13 WEEK 14 WEEK 15 WEEK 16 WEEK 20 WEEK 24 GROUP: C93111 C93112 C93113 C93115 C93116 C93117 C93118 C93119 C93120 C93121 C93122 C93124 C93125 C93126 C93127 C93128 C93129 C93130 C93131 C93132 C93133 C93134 C93135 C93136 C93137 C93138 C93139 C93140 C93141 C93143 C93145 FEMALE 5 - 20 PPM 132.00 124.00 124.00 113.00 SPILLED 120.00 116.00 111.00 107.00 101.00 130.00 128.00 117.00 108.00 121.00 120.00 133.00 136.00 130.00 124.00 115.00 123.00 128.00 122.00 121.00 109.00 124.00 147.00 146.00 SPILLED 135.00 126.00 118.00 119.00 116.00 121.00 126.00 112.00 110.00 125.00 107.00 134.00 130.00 122.00 99.00 126.00 129.00 126.00 145.00 118.00 117.00 118.00 125.00 131.00 126.00 126.00 122.00 120.00 142.00 139.00 180.00 119.00 115.00 109.00 107.00 111.00 111.00 108.00 116.00 105.00 117.00 99.00 102.00 141.00 120.00 96.00 112.00 127.00 122.00 131.00 121.00 111.00 106.00 114.00 131.00 119.00 123.00 116.00 121.00 129.00 145.00 184.00 SPILLED NOT TAKEN 119.00 110.00 113.00 110.00 117.00 123.00 103.00 102.00 97.00 127.00 106.00 123.00 105.00 NOT TAKEN 123.00 131.00 132.00 121.00 118.00 122.00 120.00 115.00 127.00 130.00 114.00 123.00 136.00 132.00 NOT TAKEN 131.00 114.00 129.00 109.00 117.00 SPILLED 125.00 110.00 124.00 100.00 128.00 104.00 118.00 109.00 121.00 119.00 140.00 119.00 123.00 121.00 120.00 142.00 131.00 130.00 116.00 119.00 150.00 139.00 143.00 119.00 124.00 110.00 116.00 120.00 135.00 103.00 110.00 100.00 129.00 118.00 117.00 109.00 123.00 136.00 139.00 SPILLED 124.00 128.00 120.00 130.00 135.00 125.00 121.00 117.00 155.00 156.00 145.00 SPILLED 119.00 112.00 118.00 SPILLED 134.00 111.00 121.00 108.00 129.00 117.00 124.00 117.00 122.00 163.00 137.00 126.00 143.00 SPILLED 132.00 121.00 136.00 135.00 126.00 SPILLED 155.00 143.00 123.00 120.00 114.00 112.00 122.00 123.00 128.00 105.00 120.00 104.00 123.00 122.00 105.00 113.00 116.00 160.00 133.00 129.00 129.00 SPILLED 117.00 102.00 144.00 123.00 121.00 114.00 147.00 132.00 SPILLED Covance 6329-183 3M T-6295 PAGE: 45 WEEK 28 WEEK 32 127.00 111.00 105.00 115.00 131.00 93.00 108.00 119.00 99.00 138.00 105.00 109.00 121.00 107.00 118.00 114.00 128.00 128.00 SPILLED 121.00 116.00 139.00 121.00 119.00 117.00 139.00 144.00 163.00 130.00 123.00 101.00 124.00 145.00 127.00 110.00 116.00 98.00 159.00 101.00 108.00 123.00 130.00 133.00 145.00 130.00 127.00 121.00 113.00 144.00 146.00 120.00 110.00 119.00 152.00 129.00 SPILLED 1390 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WE1E1K WE12EK WEEK 13 WEEK 14 WEEK 15 WEEK 16 WE20EK WEEK 24 GROUP: C93146 C93147 C93148 C93149 C93150 C93151 C93152 C93153 C93154 C93155 C93156 C93157 C93158 C93160 C93161 C93162 C93163 C93164 C93165 C93166 C93167 C93168 C93169 C93170 C93171 C93172 C93173 C93174 C93175 C93176 C93177 FEMALE 5 - 20 PPM 111120..0000 143.00 124.00 SPI1L2L2E.0D0 126.00 SPI1L2L2E.0D0 SPILLED 113.00 134.00 124.00 141.00 128.00 SPILLED 109.00 114.00 109.00 130.00 125.00 108.00 122.00 SPILLED SPI1L2L0E.0D0 118.00 SPILLED 113.00 113.00 SPILLED 110291..0000 135.00 SPILLED 116210..0000 130.00 217.00 117.00 SPILLED 108.00 139.00 124.00 153.00 124.00 SPILLED 112.00 116.00 116.00 138.00 125.00 SPILLED 120.00 172.00 SPILLED 123.00 117.00 SPILLED 123.00 115.00 SPILLED 112.00 116.00 143.00 SPILLED 150.00 116.00 125.00 SPI1L2L0E.0D0 210.00 108.00 123.00 128.00 147.00 SPILLED 123.00 111.00 111120..0000 128.00 126.00 114.00 116.00 126.00 117.00 132.00 SPILLED SPILLED 109.00 115.00 113.00 SPILLED 116.00 131.00 148.00 129.00 117.00 129.00 SPILLED 114.00 NOT TAKEN 110250..0000 126.00 148.00 111070..0000 110.00 106.00 111270..0000 112281..0000 NOT TAKEN SPILLED SPILLED SPI1L1L0E.0D0 SPILLED 115.00 117.00 109.00 102.00 119.00 113272..0000 115211..0000 131.00 SPI1L2L0E.0D0 107.00 112252..0000 150.00 136.00 118.00 110.00 115.00 115.00 139.00 127.00 117.00 168.00 136.00 123.00 115.00 SPI11L11L22E..00D00 115.00 111210..0000 137.00 119.00 133.00 117.00 123.00 SPILLED 119.00 107.00 124.00 128.00 146.00 123.00 SPILLED 110291..0000 113.00 124.00 129.00 124.00 111522102...000000 117.00 SPI1L1L2E.0D0 111182..0000 112251..0000 144.00 124.00 144.00 128.00 SPILLED SPILLED 115.00 108.00 132.00 136.00 152.00 126.00 SPILLED 143.00 113.00 115.00 136.00 124.00 SPILLED SPILLED 116.00 126.00 113.00 SPI1L1L2E.0D0 128.00 SPILLED 101.00 116.00 153.00 113.00 139.00 125.00 SPILLED 124.00 109.00 106.00 118.00 133.00 137.00 119.00 SPILLED 131.00 111140..0000 146.00 113052..0000 SPI1L1L1E.0D0 136.00 137.00 SPILLED 113.00 126.00 146.00 Covance 6329-183 3M T-6295 PAGE: 46 WEEK 28 WEEK 32 18151..0000 114130..0000 142.00 117.00 SPILLED 136.00 108.00 108.00 130.00 134.00 170.00 126.00 129.00 111011910...000000 SPILLED 112191..0000 220.00 123.00 134.00 115.00 SPILLED 105.00 112142..0000 110131..0000 113250..0000 137.00 116.00 131.00 114.00 117.00 111262..0000 134.00 178.00 125.00 136.00 109.00 114.00 111122612...000000 115.00 190.00 113222..0000 105.00 SPILLED 110290..0000 110.00 1391 Covance 6329-183 3M T-6295 1392 ft H o H ro !S 1 1 i i i i ooo ooo LO [> Ol O rH ro rH rH rH OOOOOOOOOOOOOOOOOOOOOOOOO oOOOOOOOOOOOOOOOOOOOOOOoO LO LO l> Ol CO O LO rH LO Ol rH l> ro ftft ftLO CO l> l> O ro LO LO Ol O rH rH Ol ro ro rH Ol ro Ol rH ft Ol rH rH LO o ro rH rH rH rH rH rH rH rH rH rH rH rH rH rH rH rH rH rH rH rH rH rH rH rH rH rH ft H co H o !S 1 1 i i i i oOO oOO CO ro rH o rH ro rH rH rH OOOOOOOOOOOOOOOOOOOOOOOOO OOOOOOOOOOOOOOOOOOOOOOOOO ft ft ft ftftOl LO LO LO ft ft ftOl O Ol rH LO CO CO O CO LO LO LO rH ro LO LO ro O ro Ol rH ro Ol Ol rH rH O rH LO ro CO rH rH rH rH rH rH rH rH rH rH rH rH rH rH rH rH rH rH rH rH rH rH rH rH rti TtJ df)t ft 3cto o u T5 O C fOt rti g Tt5 Eh fEfhiCft fftt H Ol & OCOh H o ro HHH o o o o o o o o o o o o o o o o ooo oooo ooooooooooooooooQoooQoooo [ >.-..t.<.L.O.r H..r o.i.>.o .i c.o.L.O.i .> .o o.i.L.o.o .[ >.L OftP i >c oo p*ftslt <[ >c oi > (NOCNCN^OLnrOHCN^H^CNHCNPHOroporOHH ft ftH r l H r l r l H r l r l r l r l r l r l r l r l r l r l H r l r l H H H r l r l r l ww i-- i 1 >h 1 - - 1 LO 1 ft 1 Ol 1 LO 1 11 EH 1 1 - 1 Ui i Oi ft i ft i O '-i 5 Eh P ft < r i! w i i i u 2 SQ PP ri! u i P cU l Eh Eh IH O U ft IH XQ OM Eh U 1 i 1 1 i 1 1 1 ri U1 IH u 1 5H 1 O5 1 ft O i ft ft i UP i ftP PU } 1i CEh 2ft fMt <Eh U ft OO ft ft ft O &P iP *F ft o ft H ft ft ft Ho H Ol OOO OOO LO Ol rH Ol Ol LO rH rH rH ft H LO H rH OOO OOO CO ro CO rH ro ro rH rH rH ft W LO WH !s 1 1 1 1 11 1 1 o OO o OO LO LO CO O l rH rH rH rH ft H^ HH P i i i i 1 ii i i o OO o OO ro rH l> o rH ro rH rH rH ft H ro HH P 1 1 1 i 1 ii i i i o OO o OO ftLO rH ro ro rH rH rH ft W Ol w rH i i i 1 1 1 1 1 1 1 ft W rH W rH 1 1 1 1 1 1 1 o OO o OO ft2 LO [> ro rH OI ro f t rH rH rH o Ol LO O O O OOO H P LO LO LO ri rH OI OI 2 rH rH rH OOOOOOOOOOO O O OOO OOOO OOOOOOOOOOOnOnnOnOOOnOOOO LO ro O O CO ftO Ol ro CO w p ww w w p p O p O [> LO p LO LO Ol ro Ol ro Ol ro Ol [> Ol Ol ro p p p Ol p ro rH p rH LO Ol ro ft ftft ft ftrH rH rH rH rH rH rH rH rH rH rH IH rH IH IH rH IH rH rH rH IH rH rH rH rH Ul Ul Ul Ul Ul OOOOOO OOOOoOooOoOO OOOO OOOOOOnOOOOoOooOoOOnnOOOO w coOl LO LO LO ro Ol p CO rH ro ro o rH [>Ol O Ol rH rH p ro Ol Ol ro Ol ro ww LO O o Ol p p rH Ol o Ol rH p p ro ro Ol Ol ftftrH rH rH rH rH rH IH rH rH rH rH rH rH rH rH rH rH rH rH IH IH rH rH rH rH ui w OOOOOOOOOOOOOOOOOOOOOOOOO OOOOOOOOOOOOOOOOOOOOOOOOO LO LO LO LO O O LO LO r o O l> O r o CO rH CO CO rH O r o LO LO O l CO O l rH r o rH r o rH r o O l rH r o O l O l O l LO O l rH O O l rH CO O l O Ol ro rH rH rH rH rH rH rH rH rH rH rH rH rH rH rH rH rH rH rH rH rH rH rH rH rH OOOOOOOOOOOOO OOOOO O OOO OOOOOOOOOOOOOo OOOOo o OO ftO l l> r o l> LO l> rH ftH P o LO rH LO HH P [> P LO [> r o O l rH O l rH ro rH O l O l ro O l ro rH ro P O l O l rH O l O P rH P ro O l ro ft ft ftrH rH rH rH rH rH rH rH rH rH rH rH rH H rH rH rH rH rH M rH M rH rH rH Ul Ul Ul OOOO OOOOOOOOOOOOOO O OOO OOOOo OOOOOOOOOOOOOo o OO ft H O l rH O l P LO CO rH LO HH CO LO LO LO rH LO CO r o CO P LO P LO ft r o rH r o rH P O O l r o rH O l O l rH O l LO O l rH O r o rH P O l P rH O l O l ft ft ftrH rH rH rH M rH rH rH rH rH rH rH rH rH rH rH rH rH rH M rH M rH rH rH Ul Ul Ul o OOOOOOOOOOOOOOOOOO OOOOO o OOOOOOOOOOOOOOOOOOn OOOOO ft ft2 LO CO CO r o O LO L-" r o CO O I L-" LO LO O O LO r o w p LO r o r o O I O O I rH O O I O I rH r o O I O I O I LO O I rH rH r o rH p r o rH O I O I r o ftf t rH rH rH rH rH rH rH rH rH rH rH rH rH rH rH rH rH rH rH H rH rH rH rH rH o Ul OI LO O O O O O O O O O O O O O O O O O O O OOOOO OOOOOOOOOOOOOOOOOOOn OOOOO ftW P L-" LO LO r o L-" LO O I rH CO rH r o CO rH f t ro LO L-" w p rH CO LO CO CO c 2 O I O O I rH O I O r o O I O I O I O I O I O I LO O I rH O rH rH p rH O O I O I LO rH rH rH rH rH rH rH rH rH rH rH rH rH rH rH rH rH rH rH H rH rH rH rH rH p ft C2 h ft f2t 2P <! 5 CO f t o I> I> CO HHH ro ro ro ft ft ft UUU H 0 ir0-t<L0L0l>c0ftOrH0ir0-t<L0L0l>c0ftOrH0ir0-t<L0 cococococococococoftftftftftftftftftftoooooo HHHHHHHHHHHHHHHHHHHOJOJtNOlOJO] rfotfr otfr otfr tofr tofr tofr tofr tofr tofr tofr tofr tofr tofr tofr tofr otfr otfr otfr otfr otfr otfr otfr otfr otfr ot UUUUUUUUUUUUUUUUUUUUUUUUU Covance 6329-183 3M T-6295 1393 1 1 1 1 1 1 1 1 1 1 1 W O 1 1 Cft 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 W1 m HC HM f t ii i i & M i1 X1 u .i p H LO ft 1 1 W Ol 1 LO 1 X 1i HM H iI U *> I MW I O I ft ft I rd Q ft I < ) -- M H i I i UP i ft C i <! U1 u 1 i TtJ df)t dCtO o u Cft o fot rti 2 p r. fMt W W XC HM U H <f t) M X <) M H U C U MU M i 1i 1 1 i i ii i 1 1 1 i i 1 1 <) i T5 d ft m <f t) ii u ft i X ft W ft i 11 <! H 1 H fMt < H UU ii i i <) i ft O 1 ft ft 1 ft <) 1 & ft ft ft O ft rH f t ft 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 ffffttttOr ol 1 1 1 i i oooooooooooooo oooooooooooooo ftrH r o r o rH CO r o o o (N L-" o r o CN o CO f t r o rH rH rH r o r o rH o o U) LO i rH rH rH rH rH rH rH rH rH rH rH rH rH rH i i i i i i i OOOOOo OOOOOOOOO ffffttttCOOl i 1 1 i OOOOOo OOOOOOOOO ft LO LO CO r o L-" (N LO (N O (N (N rH O LO r o f t (N O (N rH CN r o r o CN r o i rH rH rH rH rH rH rH rH rH rH rH rH rH rH i i i i i i i OO Oo O OOO O ft i nO O nO o O nO O O nnO n ft i ww w ww w ft Ol 1 ft 1 1 p (N rH p (N [> (N p LO [> r o p p p p rH O p r o f t (N p CN rH (N p p CN p H rH rH H rH rH M rH rH rH H M rH M 1 ft ft ft ft ft ft 1 1 ui w w ww w 1 1 1 1 OO oO OOO O ft 1 OOo OOOOO ft O ft Ol ft 1 1 1 1 w p ft w p w p (N O w p [> f t O w p w p [> w p p CN O p p rH p CN (N CN p p r o p M rH rH M H rH rH M rH rH rH M M rH M 1 ft ft ft ft ft ft ft 1 1 Ul w w w ww w 1 1 1 1 OO OOOo OOOo o O ft 1 n n nO O OOOOOOOOOO f t LO f t rH ft 1 1 1 1 w p ft w p ro ro LO LO CO LO w p p rH O p ro rH ro o (N rH O (N (N p M rH rH M rH rH rH rH rH rH rH rH rH rH H 1 ft ft ft 1 1 ui w w 1 1 1 1 OOOOOOOOOOO OOO ft 1 nO O O O O O O O O O O OOO w LO w rH ft 1 1 1 1 LO rH O LO w CO CO L-" r o p U ) r o (N rH rH rH (N O r o O rH rH rH p r o (N CO rH rH rH rH rH rH rH rH rH rH rH M rH rH rH 1 ft 1w 1 1 1 1 1 OO OOOOOOO O ft 1 nO O nO O O O O O O nnO n w w rH ft 1 1 1 1 w p ro O w p LO r o LO f t rH CO LO w p w p ft w p p rH rH p CN rH (N rH (N rH O p p CN p H rH rH M rH rH rH rH rH rH rH H H rH H 1 ft ft ft ft ft 1 1 ui w ww w 1 1 1 1 OOO OOOOOOO OOO ft 1 n nO O O OOOOOOO OOO w ro w rH ft i 1 1 1 ftro LO w p O CO O w LO LO [> rH p 1> rH O rH (N O p O (N (N rH rH rH p r o LO rH rH rH M rH rH rH rH rH rH rH M rH rH rH 1 ft ft 1w 1 w 1 1 1 1 OO OOOOOOO OO ft 1 nO O nO O O O O O O nO O n w (N 1 ww ww ft ftw rH ft 1 1 1 2 ft ft p ro LO p LO ro [> ro CO (N rH p LO [> p p ro rH p (N rH ro rH rH CN rH p ro O p H rH rH H rH rH rH rH rH rH rH M rH rH M 1 ft ft 1o ui 1 (N w ww 1 ft wHftrr H H 1 1 1 1 1 1 1 1 1 1 nLO O O O OOO OOOOOOOOOOO OOOOOOOOOOO W ftP l> O w (N p (N CO LO rH O ro L-" L-" c OCN (N (N p CN LO (N (N (N rH ro ro LO 2Wft fwtrH rH rH M rH rH rH rH rH rH rH rH rH rH rH 1 1 ft ft ft1 P ft 1 1 CW i POfot [> O 1> OLO CO rH (N ro LO LO CO O O O O rH rH rH rH rH rH rH rH rH rH (N 2M PQ 2 1 i 2 D 1 (N (N (N (N CN (N (N (N (N CN (N (N (N (N CN ftro ro ro ro ro ro ro ro ro ro ro ro ro ro ro ft ft ft ft ft ft ft ft ft ft ft ft ft ft <! i UUUUUUUUUUUUUUU Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 36 WEEK 40 WEEK 44 WEEK 48 WEEK 52 WEEK 56 WEEK 60 WEEK 64 GROUP: C92501 C92502 C92503 C92504 C92505 C92507 C92508 C92510 C92512 C92513 C92514 C92515 C92516 C92517 C92518 C92519 C92520 C92522 C92523 C92525 C92527 C92530 C92531 C92533 C92534 C92535 C92536 C92537 C92538 C92539 C92540 C92541 MALE 1 - 0 192.00 168.00 168.00 166.00 188.00 158.00 167.00 190.00 183.00 189.00 204.00 161.00 161.00 206.00 214.00 160.00 182.00 163.00 174.00 175.00 171.00 225.00 189.00 196.00 173.00 183.00 176.00 209.00 216.00 210.00 210.00 190.00 PPM 182.00 167.00 170.00 167.00 205.00 164.00 164.00 179.00 194.00 186.00 202.00 163.00 161.00 212.00 217.00 159.00 184.00 163.00 177.00 176.00 172.00 206.00 165.00 198.00 154.00 180.00 165.00 213.00 225.00 206.00 199.00 210.00 196.00 159.00 172.00 175.00 188.00 163.00 164.00 189.00 183.00 188.00 186.00 104.00 170.00 207.00 208.00 155.00 187.00 173.00 177.00 175.00 178.00 SPILLED 145.00 197.00 167.00 110.00 177.00 216.00 231.00 187.00 208.00 193.00 197.00 162.00 167.00 186.00 180.00 157.00 178.00 179.00 175.00 193.00 189.00 173.00 206.00 208.00 153.00 193.00 154.00 171.00 178.00 182.00 209.00 SPILLED 191.00 171.00 196.00 172.00 218.00 237.00 209.00 200.00 200.00 197.00 164.00 83.00 156.00 182.00 159.00 179.00 169.00 129.00 194.00 176.00 170.00 204.00 214.00 152.00 178.00 173.00 177.00 165.00 169.00 224.00 182.00 197.00 135.00 198.00 186.00 SPILLED 231.00 204.00 206.00 192.00 172.00 203.00 195.00 200.00 SPILLED 193.00 189.00 172.00 222.00 164.00 186.00 182.00 180.00 183.00 227.00 SPILLED 193.00 203.00 195.00 239.00 252.00 200.00 211.00 170.00 190.00 190.00 191.00 206.00 186.00 164.00 169.00 233.00 173.00 197.00 173.00 44.00 172.00 236.00 SPILLED 204.00 197.00 189.00 220.00 261.00 208.00 216.00 174.00 179.00 192.00 185.00 205.00 184.00 229.00 173.00 220.00 183.00 177.00 175.00 48.00 195.00 190.00 168.00 198.00 212.00 195.00 243.00 225.00 202.00 210.00 Covance 6329-183 3M T-6295 PAGE: 49 WEEK 68 WEEK 72 181.00 182.00 191.00 184.00 207.00 195.00 231.00 175.00 143.80 160.50 186.70 172.60 207.20 194.90 219.70 176.40 235.00 187.00 139.00 190.00 130.00 193.00 226.00 NOT TAKEN 201.00 204.00 183.00 241.00 243.00 210.00 217.00 228.10 189.10 SPILLED 168.00 NOT TAKEN 196.00 227.90 211.80 192.40 190.60 204.40 250.10 210.10 213.40 1394 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 36 WEEK 40 WEEK 44 WEEK 48 WEEK 52 WEEK 56 WEEK 60 WEEK 64 GROUP: MALE 1 - 0 PPM C92542 C92543 C92544 C92545 C92547 C92548 C92549 C92550 C92551 C92552 C92553 C92554 C92555 C92556 C92557 C92558 C92559 C92560 C92561 C92562 C92563 C92564 C92565 C92567 C92568 C92569 C92570 185.00 165.00 180.00 183.00 179.00 184.00 154.00 225.00 166.00 185.00 140.00 154.00 194.00 176.00 189.00 187.00 178.00 190.00 180.00 181.00 202.00 196.00 190.00 175.00 186.00 162.00 179.00 187.00 168.00 182.00 182.00 181.00 183.00 161.00 219.00 168.00 196.00 SPILLED 154.00 205.00 165.00 181.00 194.00 183.00 200.00 181.00 175.00 237.00 195.00 187.00 178.00 185.00 168.00 183.00 193.00 163.00 184.00 213.00 184.00 183.00 162.00 224.00 161.00 192.00 124.00 154.00 205.00 184.00 192.00 208.00 180.00 201.00 176.00 178.00 240.00 195.00 195.00 179.00 186.00 175.00 175.00 200.00 172.00 164.00 199.00 180.00 178.00 166.00 216.00 155.00 204.00 130.00 157.00 188.00 183.00 171.00 189.00 175.00 198.00 175.00 183.00 233.00 194.00 188.00 166.00 184.00 160.00 173.00 185.00 166.00 177.00 191.00 173.00 177.00 157.00 215.00 159.00 199.00 102.00 150.00 202.00 168.00 180.00 SPILLED 178.00 210.00 178.00 142.00 SPILLED SPILLED 183.00 175.00 183.00 175.00 128.00 188.00 179.00 173.00 197.00 189.00 190.00 167.00 228.00 170.00 211.00 124.00 156.00 152.00 96.00 196.00 231.00 191.00 200.00 186.00 SPILLED 193.00 192.00 175.00 193.00 178.00 211.00 179.00 175.00 211.00 190.00 192.00 169.00 221.00 169.00 202.00 100.00 161.00 NOT TAKEN 56.00 195.00 198.00 190.00 208.00 141.00 SPILLED 198.00 206.00 193.00 189.00 172.00 205.00 182.00 181.00 206.00 199.00 181.00 172.00 212.00 177.00 173.00 149.00 193.00 196.00 185.00 205.00 252.00 192.00 193.00 186.00 191.00 181.00 Covance 6329-183 3M T-6295 PAGE: 50 WEEK 68 WEEK 72 200.00 180.00 190.00 208.00 209.00 169.00 170.00 221.00 190.00 216.00 171.00 200.00 197.00 190.00 222.00 240.00 139.00 181.00 192.00 200.00 175.00 199.70 175.90 177.60 220.60 199.10 167.30 169.90 224.80 193.90 202.90 167.40 197.40 169.30 191.80 221.90 261.00 152.40 197.80 211.00 174.90 1395 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 36 WEEK 40 WEEK 44 WEEK 48 WEEK 52 WEEK 56 WEEK 60 WEEK 64 GROUP: C92571 C92572 C92573 C92576 C92577 C92578 C92579 C92580 C92581 C92582 C92583 C92584 C92585 C92586 C92587 C92588 C92589 C92590 C92591 C92592 C92594 C92595 C92596 C92597 C92598 C92599 C92601 C92602 C92604 C92605 C92606 MALE 2 - 0. 5 PPM 185.00 200.00 189.00 200.00 208.00 187.00 195.00 178.00 190.00 153.00 163.00 174.00 36.00 206.00 209.00 172.00 186.00 187.00 169.00 183.00 179.00 166.00 189.00 177.00 185.00 208.00 182.00 170.00 192.00 187.00 180.00 190.00 200.00 160.00 194.00 193.00 199.00 185.00 180.00 183.00 150.00 167.00 164.00 210.00 190.00 157.00 181.00 190.00 169.00 174.00 179.00 156.00 181.00 187.00 187.00 211.00 181.00 155.00 205.00 195.00 189.00 182.00 193.00 208.00 187.00 199.00 194.00 191.00 198.00 189.00 154.00 169.00 190.00 208.00 203.00 175.00 194.00 188.00 164.00 187.00 174.00 155.00 190.00 185.00 185.00 217.00 184.00 166.00 194.00 174.00 188.00 191.00 197.00 212.00 197.00 190.00 196.00 187.00 188.00 189.00 170.00 166.00 191.00 196.00 196.00 166.00 192.00 192.00 179.00 181.00 183.00 159.00 191.00 195.00 189.00 210.00 178.00 177.00 204.00 181.00 184.00 182.00 172.00 196.00 192.00 199.00 192.00 195.00 190.00 193.00 154.00 178.00 SPILLED 198.00 192.00 162.00 195.00 193.00 172.00 183.00 176.00 169.00 193.00 185.00 180.00 196.00 181.00 174.00 204.00 180.00 186.00 186.00 208.00 212.00 215.00 215.00 216.00 199.00 203.00 214.00 155.00 185.00 SPILLED 206.00 214.00 177.00 204.00 197.00 180.00 203.00 188.00 167.00 193.00 181.00 179.00 188.00 174.00 205.00 200.00 199.00 202.00 214.00 201.00 213.00 210.00 214.00 209.00 212.00 216.00 167.00 179.00 NOT TAKEN 188.00 211.00 182.00 204.00 199.00 172.00 197.00 198.00 170.00 190.00 176.00 185.00 178.00 193.00 214.00 191.00 196.00 193.00 SPILLED 196.00 211.00 204.00 210.00 195.00 206.00 217.00 161.00 189.00 204.00 199.00 186.00 200.00 194.00 182.00 195.00 180.00 173.00 173.00 167.00 177.00 186.00 194.00 211.00 202.00 190.00 Covance 6329-183 3M T-6295 PAGE: 51 WEEK 68 WEEK 72 203.00 214.00 115.00 216.00 207.00 219.00 188.00 228.00 231.00 SPILLED 180.00 50.00 210.00 181.00 211.00 207.00 189.00 203.00 206.00 155.00 41.00 180.00 174.00 200.00 202.00 199.00 189.00 195.00 173.20 207.20 193.10 215.70 203.20 223.70 164.80 144.50 241.10 161.80 193.60 0.50 197.20 169.20 200.90 196.30 182.00 203.00 189.00 174.00 186.00 185.00 188.00 197.00 154.00 205.00 210.00 1396 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 36 WEEK 40 WEEK 44 WEEK 48 WEEK 52 WEEK 56 WEEK 60 WEEK 64 GROUP: MALE 2 - 0 .5 PPM C92607 C92608 C92609 C92611 C92612 C92614 C92615 C92617 C92619 C92620 C92622 C92623 C92624 C92625 C92626 C92628 C92629 C92630 213.00 164.00 183.00 160.00 186.00 SPILLED 190.00 209.00 147.00 160.00 187.00 151.00 195.00 201.00 140.00 217.00 212.00 209.00 219.00 205.00 196.00 156.00 175.00 SPILLED 195.00 214.00 155.00 162.00 175.00 148.00 195.00 197.00 189.00 SPILLED 214.00 212.00 GROUP: MALE 3 - 2 PPM C92631 C92632 C92633 C92634 C92635 C92636 C92637 C92638 C92639 C92641 177.00 207.00 175.00 174.00 186.00 174.00 166.00 223.00 177.00 180.00 173.00 195.00 192.00 172.00 186.00 174.00 168.00 227.00 178.00 211.00 209.00 188.00 202.00 153.00 178.00 246.00 186.00 216.00 163.00 154.00 184.00 142.00 186.00 199.00 169.00 239.00 215.00 207.00 176.00 193.00 176.00 185.00 190.00 192.00 163.00 213.00 179.00 195.00 207.00 219.00 189.00 161.00 176.00 245.00 195.00 203.00 175.00 171.00 186.00 152.00 201.00 204.00 197.00 244.00 216.00 207.00 192.00 223.00 193.00 160.00 176.00 SPILLED 189.00 202.00 154.00 175.00 185.00 154.00 204.00 189.00 199.00 120.00 210.00 202.00 219.00 217.00 202.00 165.00 195.00 SPILLED 196.00 232.00 SPILLED 171.00 196.00 158.00 211.00 211.00 167.00 SPILLED 224.00 215.00 242.00 211.00 196.00 160.00 194.00 261.00 195.00 227.00 176.00 166.00 201.00 153.00 207.00 210.00 171.00 55.00 218.00 197.00 226.00 191.00 192.00 171.00 191.00 SPILLED 175.00 212.00 169.00 174.00 198.00 158.00 201.00 194.00 SPILLED 203.00 206.00 176.00 201.00 188.00 184.00 183.00 203.00 171.00 235.00 180.00 189.00 168.00 197.00 176.00 181.00 190.00 217.00 171.00 222.00 178.00 213.00 178.00 204.00 186.00 200.00 193.00 226.00 168.00 221.00 188.00 190.00 177.00 180.00 192.00 202.00 198.00 SPILLED 187.00 236.00 196.00 118.00 181.00 157.00 193.00 196.00 171.00 SPILLED 182.00 223.00 196.00 187.00 Covance 6329-183 3M T-6295 PAGE: 52 WEEK 68 WEEK 72 234.00 184.00 195.00 176.00 185.00 SPILLED 204.00 251.00 181.00 183.00 208.00 157.00 200.00 215.00 110.00 180.00 195.00 226.00 81.00 196.00 165.00 147.00 SPILLED 201.00 261.00 177.30 162.30 204.00 150.90 196.60 147.80 107.40 129.80 208.90 189.00 195.00 203.00 194.00 198.00 SPILLED 190.00 227.00 183.00 208.00 188.00 226.00 190.00 203.00 179.00 SPILLED 179.00 220.00 139.00 172.00 1397 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 36 WEEK 40 WEEK 44 WEEK 48 WEEK 52 WEEK 56 WEEK 60 WEEK 64 GROUP: MALE 3 - 2 PPM C92642 C92643 C92644 C92647 C92648 C92649 C92651 C92653 C92654 C92655 C92656 C92657 C92658 C92659 C92660 C92661 C92663 C92664 C92665 C92666 C92667 C92669 C92670 C92671 C92672 C92673 C92674 C92675 C92677 C92679 C92680 161.00 167.00 166.00 161.00 161.00 189.00 187.00 195.00 184.00 199.00 177.00 187.00 190.00 195.00 195.00 151.00 203.00 186.00 191.00 222.00 196.00 179.00 160.00 187.00 164.00 164.00 170.00 183.00 184.00 206.00 176.00 158.00 166.00 164.00 168.00 156.00 179.00 189.00 198.00 180.00 218.00 179.00 176.00 189.00 203.00 SPILLED 171.00 201.00 188.00 195.00 214.00 204.00 186.00 161.00 185.00 182.00 168.00 182.00 187.00 186.00 207.00 186.00 160.00 168.00 159.00 172.00 165.00 190.00 193.00 199.00 169.00 205.00 178.00 177.00 193.00 214.00 203.00 170.00 203.00 188.00 201.00 196.00 195.00 177.00 159.00 181.00 169.00 178.00 180.00 183.00 178.00 221.00 178.00 167.00 184.00 168.00 171.00 152.00 SPILLED 203.00 187.00 167.00 209.00 172.00 175.00 194.00 207.00 215.00 171.00 207.00 192.00 189.00 193.00 195.00 191.00 157.00 185.00 172.00 162.00 171.00 184.00 188.00 239.00 185.00 172.00 182.00 168.00 166.00 154.00 185.00 216.00 207.00 172.00 210.00 176.00 181.00 188.00 212.00 SPILLED 178.00 210.00 186.00 189.00 193.00 184.00 180.00 154.00 178.00 178.00 166.00 192.00 183.00 182.00 206.00 182.00 172.00 SPILLED 180.00 177.00 170.00 216.00 220.00 182.00 186.00 SPILLED 191.00 194.00 207.00 226.00 SPILLED 184.00 218.00 197.00 200.00 199.00 202.00 190.00 165.00 205.00 186.00 191.00 193.00 198.00 197.00 204.00 176.00 200.00 185.00 176.00 169.00 196.00 230.00 227.00 195.00 209.00 189.00 185.00 218.00 232.00 SPILLED 188.00 TECH SPILL 197.00 213.00 205.00 203.00 194.00 176.00 193.00 196.00 199.00 198.00 191.00 211.00 161.00 SPILLED 180.00 183.00 172.00 176.00 198.00 157.00 213.00 203.00 196.00 191.00 205.00 227.00 206.00 203.00 SPILLED 194.00 SPILLED 194.00 202.00 200.00 198.00 178.00 202.00 201.00 196.00 198.00 201.00 196.00 189.00 Covance 6329-183 3M T-6295 PAGE: 53 WEEK 68 WEEK 72 176.00 SPILLED 183.00 189.00 167.00 210.00 186.00 223.00 190.00 231.00 195.00 191.00 209.00 220.00 193.00 201.00 236.00 207.00 218.00 122.00 215.00 194.00 189.00 205.00 199.00 203.00 206.00 196.00 216.00 97.00 218.00 215.00 192.00 195.00 165.00 206.00 152.00 220.00 188.00 227.00 191.00 193.00 202.00 240.00 179.00 196.00 216.00 172.00 221.00 113.00 205.00 211.00 150.00 196.00 218.00 195.00 202.00 196.00 195.00 104.00 1398 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 36 WEEK 40 WEEK 44 WEEK 48 WEEK 52 WEEK 56 WEEK 60 WEEK 64 GROUP: MALE 3 - 2 PPM C92681 C92682 C92683 C92685 C92686 C92687 C92688 C92689 C92690 175.00 175.00 184.00 178.00 184.00 148.00 195.00 154.00 203.00 186.00 180.00 193.00 184.00 192.00 211.00 SPILLED 177.00 207.00 GROUP: MALE 4 - 5 PPM C92691 C92692 C92693 C92694 C92695 C92696 C92697 C92698 C92699 C92700 C92701 C92702 C92703 C92704 C92705 C92706 C92707 C92708 C92709 177.00 158.00 174.00 177.00 175.00 188.00 193.00 178.00 168.00 184.00 169.00 167.00 197.00 172.00 185.00 172.00 154.00 161.00 152.00 173.00 169.00 162.00 178.00 178.00 178.00 183.00 188.00 161.00 174.00 176.00 171.00 209.00 172.00 198.00 192.00 161.00 165.00 174.00 185.00 177.00 190.00 179.00 187.00 184.00 SPILLED 171.00 205.00 177.00 134.00 179.00 172.00 169.00 177.00 191.00 191.00 170.00 177.00 179.00 168.00 184.00 169.00 196.00 201.00 167.00 173.00 173.00 176.00 180.00 191.00 187.00 196.00 157.00 SPILLED 169.00 225.00 179.00 139.00 178.00 172.00 171.00 187.00 192.00 197.00 160.00 167.00 162.00 165.00 198.00 176.00 193.00 203.00 164.00 169.00 179.00 183.0 179.0 192.0 177.0 192.0 181.0 SPILLED 155.00 224.00 195.00 190.00 178.00 201.00 215.00 175.00 SPILLED NOT TAKEN 234.00 193.00 198.00 191.00 204.00 227.00 178.00 197.00 231.00 171.00 NOT TAKEN 175.00 174.00 171.00 186.00 183.00 199.00 167.00 168.00 35.00 169.00 203.00 175.00 192.00 198.00 164.00 168.00 162.00 172.00 177.00 180.00 164.00 189.00 193.00 205.00 173.00 178.00 177.00 210.00 182.00 185.00 209.00 168.00 157.00 167.00 197.00 185.00 191.00 190.00 192.00 194.00 205.00 192.00 SPILLED 197.00 65.00 189.00 195.00 238.00 191.00 164.00 187.00 193.00 190.00 217.00 203.00 231.00 120.00 184.00 237.00 180.00 185.00 182.00 185.00 199.00 111.00 221.00 175.00 SPILLED 192.00 178.00 187.00 205.00 174.00 170.00 174.00 Covance 6329-183 3M T-6295 PAGE: 54 WEEK 68 WEEK 72 185.00 196.00 216.00 196.00 222.00 48.00 179.00 233.00 178.00 193.00 206.00 132.00 226.00 NOT TAKEN SPILLED 235.00 186.00 181.00 194.00 183.00 180.00 224.00 176.00 181.00 190.00 189.00 194.00 233.00 183.00 187.00 171.00 181.00 176.00 195.00 185.00 197.00 219.00 185.00 176.00 198.00 201.00 192.00 246.00 181.00 172.00 164.00 1399 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 36 WEEK 40 WEEK 44 WEEK 48 WEEK 52 WEEK 56 WEEK 60 WEEK 64 GROUP: MALE 4 - 5 PPM C92710 C92711 C92712 C92716 C92717 C92720 C92721 C92723 C92724 C92725 C92726 C92727 C92729 C92730 C92732 C92733 C92735 C92736 C92737 C92738 C92739 C92740 C92741 C92742 C92743 C92745 C92746 C92747 C92748 C92749 C92750 173.00 168.00 202.00 185.00 191.00 200.00 160.00 220.00 217.00 160.00 181.00 183.00 215.00 178.00 190.00 193.00 180.00 178.00 172.00 191.00 170.00 162.00 244.00 189.00 213.00 231.00 183.00 171.00 209.00 161.00 184.00 180.00 171.00 220.00 262.00 195.00 209.00 177.00 SPILLED 214.00 159.00 182.00 194.00 216.00 171.00 187.00 201.00 184.00 185.00 184.00 178.00 184.00 173.00 SPILLED 195.00 213.00 219.00 199.00 190.00 228.00 179.00 179.00 184.00 175.00 227.00 SPILLED 198.00 205.00 190.00 SPILLED 237.00 151.00 184.00 189.00 221.00 177.00 188.00 186.00 188.00 185.00 179.00 173.00 174.00 174.00 SPILLED 199.00 218.00 227.00 185.00 179.00 228.00 178.00 175.00 182.00 177.00 216.00 205.00 197.00 203.00 191.00 SPILLED 224.00 142.00 184.00 205.00 217.00 182.00 184.00 195.00 183.00 190.00 183.00 174.00 178.00 173.00 SPILLED 196.00 211.00 216.00 194.00 178.00 SPILLED 167.00 184.00 180.00 170.00 218.00 SPILLED 201.00 195.00 173.00 SPILLED 214.00 NOT TAKEN 187.00 189.00 215.00 175.00 183.00 195.00 181.00 178.00 181.00 167.00 173.00 179.00 SPILLED 193.00 209.00 225.00 192.00 179.00 SPILLED 166.00 170.00 194.00 180.00 247.00 SPILLED 205.00 207.00 180.00 SPILLED 208.00 185.00 204.00 237.00 184.00 186.00 203.00 194.00 190.00 182.00 184.00 186.00 183.00 SPILLED 198.00 235.00 234.00 207.00 193.00 228.00 171.00 192.00 190.00 180.00 246.00 SPILLED 213.00 194.00 201.00 259.00 233.00 176.00 209.00 216.00 196.00 209.00 207.00 203.00 209.00 202.00 176.00 186.00 182.00 SPILLED 207.00 221.00 244.00 204.00 195.00 231.00 170.00 186.00 182.00 189.00 217.00 119.00 216.00 211.00 196.00 208.00 222.00 181.00 199.00 186.00 198.00 193.00 198.00 193.00 196.00 192.00 181.00 180.00 176.00 SPILLED 203.00 199.00 234.00 201.00 191.00 SPILLED 170.00 188.00 Covance 6329-183 3M T-6295 PAGE: 55 WEEK 68 WEEK 72 184.00 190.00 SPILLED 402.00 221.00 212.00 195.00 243.00 235.00 188.00 198.00 141.00 190.00 193.00 207.00 184.00 197.00 192.00 191.00 188.00 189.00 SPILLED 210.00 224.00 245.00 224.00 189.00 SPILLED 183.00 192.00 177.0 0 243.0 0 SPILLEDi 230.0 0 216.0 0 196.0 0 252.0 0 222.0 0 198.0 0 197.0 0 123.0 0 200.0 0 198.0 0 208.0 0 197.0 0 185.0 0 192.0 0 195.0 0 187.0 0 197.0 0 177.0 0 196.0 0 238.0 0 231.0 0 215.0 0 192.0 0 160.0 0 176.0 0 190.0 0 1400 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 36 WEEK 40 WEEK 44 WEEK 48 WEEK 52 WEEK 56 WEEK 60 WEEK 64 GROUP: C92751 C92753 C92754 C92755 C92756 C92757 C92758 C92760 C92761 C92762 C92763 C92764 C92766 C92767 C92768 C92769 C92770 C92771 C92772 C92773 C92774 C92775 C92776 C92778 C92780 C92781 C92782 C92783 C92784 C92785 C92786 MALE 5 - 2 0 PPM 162.00 188.00 156.00 166.00 213.00 174.00 158.00 184.00 186.00 192.00 223.00 211.00 184.00 257.00 201.00 192.00 195.00 184.00 203.00 172.00 173.00 205.00 176.00 182.00 190.00 165.00 176.00 191.00 188.00 175.00 188.00 188.00 200.00 161.00 176.00 228.00 SPILLED 167.00 191.00 187.00 188.00 221.00 227.00 178.00 252.00 204.00 202.00 207.00 194.00 193.00 174.00 179.00 215.00 185.00 168.00 196.00 170.00 179.00 194.00 191.00 176.00 188.00 202.00 201.00 159.00 176.00 218.00 SPILLED 158.00 194.00 186.00 191.00 223.00 222.00 181.00 SPILLED 194.00 199.00 202.00 182.00 197.00 169.00 165.00 153.00 184.00 181.00 197.00 177.00 179.00 200.00 190.00 181.00 185.00 190.00 135.00 165.00 178.00 226.00 200.00 141.00 194.00 187.00 199.00 225.00 SPILLED 166.00 SPILLED 202.00 202.00 209.00 172.00 187.00 174.00 168.00 217.00 180.00 177.00 197.00 172.00 184.00 192.00 199.00 178.00 190.00 184.00 190.00 163.00 176.00 216.00 SPILLED 112.00 190.00 179.00 196.00 218.00 220.00 159.00 235.00 206.00 193.00 187.00 188.00 NOT TAKEN 167.00 178.00 213.00 177.00 135.00 203.00 168.00 179.00 195.00 189.00 176.00 180.00 190.00 218.00 211.00 95.00 195.00 185.00 196.00 233.00 223.00 180.00 250.00 212.00 225.00 224.00 204.00 166.00 183.00 198.00 197.00 192.00 177.00 188.00 218.00 212.00 193.00 206.00 194.00 218.00 SPILLED 89.00 209.00 180.00 201.00 236.00 231.00 201.00 SPILLED 209.00 211.00 226.00 225.00 170.00 182.00 224.00 198.00 215.00 180.00 208.00 224.00 201.00 201.00 206.00 206.00 205.00 SPILLED 196.00 186.00 208.00 213.00 215.00 180.00 297.00 198.00 199.00 209.00 202.00 166.00 169.00 226.00 165.00 210.00 194.00 199.00 209.00 221.00 187.00 204.00 Covance 6329-183 3M T-6295 PAGE: 56 WEEK 68 WEEK 72 207.00 200.00 226.00 132.00 213.00 196.00 243.00 175.00 195.00 SPILLED 202.00 213.00 209.00 208.00 178.00 183.00 228.00 199.00 232.00 200.00 204.00 222.00 217.00 203.00 216.00 212.00 225.00 209.00 69.00 200.00 208.00 235.00 84.00 183.00 279.00 201.00 215.00 207.00 206.00 167.00 176.00 218.00 190.00 223.00 22.00 200.00 197.00 237.00 206.00 203.00 1401 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 36 WEEK 40 WEEK 44 WEEK 48 WEEK 52 WEEK 56 WEEK 60 WEEK 64 GROUP: MALE 5 - 2 0 PPM C92787 C92788 C92790 C92791 C92792 C92794 C92795 C92796 C92797 C92800 C92801 C92802 C92803 C92804 C92805 C92806 C92807 C92808 C92809 C92810 C92811 C92813 C92814 C92815 C92816 C92817 C92818 C92819 C92820 209.00 188.00 164.00 190.00 168.00 166.00 176.00 161.00 198.00 165.00 216.00 187.00 178.00 181.00 162.00 148.00 172.00 162.00 175.00 180.00 NOT TAKEN 188.00 137.00 SPILLED 144.00 220.00 178.00 165.00 204.00 232.00 189.00 172.00 189.00 171.00 159.00 186.00 167.00 197.00 153.00 212.00 185.00 171.00 187.00 176.00 174.00 165.00 152.00 180.00 184.00 201.00 127.00 214.00 155.00 227.00 176.00 166.00 188.00 SPILLED 186.00 165.00 195.00 186.00 166.00 181.00 169.00 205.00 168.00 202.00 206.00 182.00 190.00 186.00 186.00 180.00 155.00 177.00 186.00 201.00 136.00 SPILLED 156.00 243.00 177.00 176.00 126.00 187.00 170.00 196.00 179.00 162.00 182.00 171.00 184.00 158.00 209.00 197.00 173.00 194.00 179.00 163.00 181.00 156.00 179.00 190.00 199.00 136.00 SPILLED 156.00 241.00 187.00 178.00 194.00 173.00 172.00 184.00 173.00 154.00 153.00 136.00 190.00 160.00 184.00 190.00 175.00 183.00 169.00 165.00 164.00 157.00 170.00 182.00 192.00 138.00 SPILLED 150.00 176.00 173.00 172.00 SPILLED 182.00 193.00 191.00 56.00 198.00 159.00 NOT TAKEN 215.00 179.00 184.00 189.00 179.00 190.00 164.00 176.00 192.00 205.00 146.00 SPILLED 169.00 195.00 187.00 207.00 208.00 189.00 196.00 195.00 195.00 213.00 182.00 222.00 188.00 210.00 214.00 201.00 201.00 172.00 189.00 217.00 207.00 148.00 223.00 173.00 193.00 187.00 204.00 180.00 209.00 179.00 204.00 184.00 202.00 185.00 170.00 183.00 204.00 216.00 141.00 TECH SPILL NOT TAKEN 187.00 184.00 Covance 6329-183 3M T-6295 PAGE: 57 WEEK 68 WEEK 72 184.00 204.00 202.00 193.00 196.00 213.00 217.00 186.00 223.00 184.00 211.00 101.00 197.00 194.00 170.00 183.00 221.00 221.00 138.00 221.00 199.00 190.00 211.00 191.00 219.00 179.00 201.00 NOT TAKEN 189.00 198.00 162.00 188.00 221.00 214.00 146.00 216.00 194.00 186.00 1402 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 36 WEEK 40 WEEK 44 WEEK 48 WEEK 52 WEEK 56 WEEK 60 WEEK 64 GROUP: C92821 C92822 C92823 C92824 C92825 C92826 C92827 C92828 C92829 C92830 C92831 C92832 C92833 C92834 C92835 C92836 C92837 C92838 C92839 C92840 C92841 C92842 C92843 C92844 C92845 C92846 C92847 C92848 C92849 C92850 C92851 MALE 6 - 2 0 PPM 165.00 162.00 179.00 198.00 234.00 189.00 190.00 183.00 208.00 165.00 166.00 158.00 196.00 182.00 165.00 174.00 191.00 183.00 259.00 158.00 159.00 186.00 194.00 140.00 178.00 202.00 216.00 156.00 215.00 SPILLED 182.00 162.00 163.00 179.00 171.00 238.00 187.00 182.00 195.00 190.00 183.00 164.00 160.00 205.00 191.00 169.00 195.00 198.00 182.00 259.00 165.00 151.00 183.00 194.00 127.00 186.00 177.00 212.00 158.00 207.00 SPILLED 189.00 151.00 165.00 186.00 189.00 249.00 192.00 191.00 196.00 231.00 189.00 169.00 161.00 207.00 195.00 172.00 192.00 210.00 189.00 257.00 163.00 164.00 191.00 199.00 143.00 188.00 211.00 210.00 167.00 216.00 SPILLED 190.00 159.00 173.00 189.00 190.00 SPILLED 202.00 189.00 205.00 214.00 192.00 171.00 165.00 220.00 196.00 170.00 185.00 210.00 186.00 SPILLED 167.00 155.00 191.00 196.00 145.00 184.00 210.00 217.00 166.00 203.00 SPILLED 186.00 155.00 174.00 179.00 190.00 SPILLED 191.00 168.00 196.00 SPILLED 141.00 174.00 158.00 198.00 195.00 154.00 184.00 201.00 173.00 238.00 160.00 151.00 175.00 188.00 145.00 174.00 196.00 213.00 152.00 223.00 SPILLED 188.00 179.00 182.00 171.00 213.00 207.00 194.00 208.00 219.00 139.00 168.00 169.00 222.00 208.00 173.00 201.00 211.00 191.00 SPILLED 168.00 173.00 162.00 SPILLED 155.00 187.00 161.00 235.00 179.00 225.00 SPILLED 203.00 164.00 SPILLED 190.00 203.00 283.00 200.00 197.00 229.00 182.00 177.00 174.00 225.00 199.00 180.00 SPILLED 129.00 214.00 265.00 174.00 178.00 196.00 222.00 149.00 191.00 207.00 233.00 153.00 199.00 228.00 206.00 184.00 186.00 189.00 199.00 SPILLED 186.00 217.00 200.00 178.00 169.00 178.00 209.00 194.00 180.00 204.00 10.00 28.00 201.00 175.00 193.00 196.00 221.00 148.00 177.00 223.00 205.00 128.00 110.00 SPILLED 201.00 Covance 6329-183 3M T-6295 PAGE: 58 WEEK 68 WEEK 72 184.00 170.00 SPILLED 205.00 SPILLED 196.00 229.00 SPILLED SPILLED 180.00 189.00 225.00 200.00 190.00 225.00 SPILLED 177.00 NOT TAKEN 192.00 230.00 160.00 186.00 200.00 232.00 139.00 46.00 198.00 206.00 197.60 127.20 176.80 160.80 288.50 192.30 212.90 233.70 172.60 188.00 217.70 194.40 172.90 215.90 242.90 171.40 199.30 239.10 163.50 194.30 204.60 238.80 133.70 197.20 197.20 1403 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 59 ANIMAL NUMBER WEEK 36 WEEK 40 WEEK 44 WEEK 48 WEEK 52 WEEK 56 WEEK 60 WEEK 64 WEEK 68 WEEK 72 GROUP: MALE 6 - 20 PPM C92852 C92853 C92854 C92855 C92856 C92857 C92858 C92859 C92860 183.00 169.00 178.00 154.00 167.00 175.00 173.00 174.00 164.00 184.00 184.00 184.00 177.00 175.00 175.00 173.00 182.00 158.00 GROUP: FEMALE 1 - 0 PPM C92862 C92863 C92865 C92866 C92867 C92868 C92869 C92870 C92871 C92872 C92873 C92874 C92875 C92876 C92877 C92878 C92879 C92881 C92882 124.00 114.00 156.00 90.00 125.00 131.00 SPILLED 143.00 130.00 SPILLED 121.00 130.00 133.00 109.00 148.00 SPILLED 145.00 143.00 117.00 131.00 132.00 141.00 116.00 158.00 132.00 SPILLED 148.00 122.00 SPILLED 138.00 139.00 154.00 120.00 118.00 SPILLED 149.00 145.00 121.00 188.00 171.00 196.00 179.00 175.00 164.00 178.00 190.00 170.00 124.00 108.00 139.00 111.00 138.00 127.00 SPILLED 147.00 124.00 SPILLED 124.00 137.00 139.00 122.00 132.00 SPILLED 145.00 145.00 131.00 190.00 177.00 186.00 156.00 180.00 186.00 191.00 191.00 165.00 123.00 125.00 138.00 147.00 138.00 132.00 147.00 135.00 127.00 SPILLED 124.00 128.00 143.00 132.00 132.00 155.00 140.00 119.00 119.00 172.00 163.00 183.00 190.00 170.00 187.00 181.00 183.00 171.00 124.00 133.00 138.00 122.00 143.00 131.00 SPILLED 142.00 129.00 167.00 137.00 139.00 144.00 109.00 126.00 SPILLED 139.00 106.00 131.00 194.00 198.00 201.00 212.00 189.00 SPILLED 202.00 189.00 170.00 132.00 150.00 119.00 149.00 149.00 SPILLED 171.00 134.00 139.00 139.00 134.00 175.00 135.00 162.00 152.00 146.00 108.00 201.00 205.00 203.00 126.00 184.00 170.00 198.00 163.00 179.00 177.00 187.00 200.00 SPILLED 187.00 SPILLED 149.00 120.00 179.00 NOT TAKEN 207.00 217.00 120.00 195.00 SPILLED 191.00 129.00 179.00 201.10 218.80 186.40 200.80 204.70 NOT TAKEN 189.70 135.00 156.00 134.00 138.00 139.00 122.00 143.00 144.00 128.00 134.00 144.00 128.00 152.00 136.00 167.00 135.00 117.00 137.00 NOT TAKEN 127.00 134.00 142.00 SPILLED 149.00 149.00 152.00 148.00 139.00 138.00 145.00 156.00 150.00 145.00 127.00 145.00 127.00 147.00 144.00 166.00 152.00 154.00 141.00 152.00 153.00 153.00 131.00 170.00 161.00 152.00 135.00 128.00 126.00 149.00 149.00 146.00 139.00 152.00 142.00 125.00 140.00 133.00 142.00 189.00 134.00 151.00 153.00 1404 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 36 WEEK 40 WEEK 44 WEEK 48 WEEK 52 WEEK 56 WEEK 60 WEEK 64 GROUP: C92883 C92884 C92885 C92886 C92888 C92889 C92890 C92891 C92892 C92893 C92894 C92895 C92896 C92897 C92899 C92900 C92901 C92902 C92904 C92906 C92907 C92908 C92909 C92910 C92911 C92912 C92913 C92915 C92916 C92917 C92918 FEMALE 1 - 0 PPM 137.00 138.00 130.00 143.00 164.00 147.00 117.00 128.00 167.00 115.00 128.00 123.00 128.00 135.00 176.00 143.00 117.00 162.00 109.00 118.00 155.00 111.00 121.00 138.00 TECH SPILL 149.00 115.00 SPILLED 154.00 153.00 136.00 170.00 146.00 139.00 149.00 153.00 101.00 134.00 132.00 147.00 113.00 132.00 113.00 137.00 144.00 171.00 151.00 119.00 160.00 143.00 122.00 105.00 135.00 114.00 SPILLED 108.00 132.00 99.00 169.00 180.00 170.00 128.00 150.00 139.00 119.00 145.00 165.00 104.00 130.00 133.00 156.00 128.00 130.00 123.00 134.00 151.00 168.00 145.00 123.00 161.00 142.00 120.00 112.00 129.00 117.00 174.00 114.00 124.00 107.00 154.00 155.00 158.00 125.00 137.00 143.00 141.00 145.00 150.00 133.00 132.00 135.00 172.00 83.00 129.00 119.00 131.00 144.00 166.00 153.00 114.00 153.00 122.00 119.00 112.00 132.00 120.00 SPILLED 119.00 179.00 119.00 144.00 158.00 149.00 135.00 121.00 141.00 137.00 119.00 167.00 106.00 158.00 130.00 144.00 129.00 145.00 110.00 121.00 151.00 135.00 163.00 118.00 169.00 105.00 112.00 112.00 134.00 107.00 150.00 124.00 170.00 113.00 159.00 160.00 160.00 133.00 160.00 145.00 140.00 154.00 178.00 143.00 146.00 153.00 127.00 126.00 132.00 168.00 165.00 131.00 187.00 133.00 133.00 SPILLED 150.00 113.00 106.00 184.00 123.00 165.00 141.00 137.00 163.00 150.00 153.00 141.00 157.00 155.00 147.00 188.00 120.00 141.00 TECH SPILL 180.00 175.00 131.00 171.00 151.00 121.00 121.00 143.00 122.00 120.00 177.00 125.00 136.00 194.00 162.00 132.00 136.00 146.00 140.00 186.00 157.00 137.00 159.00 124.00 117.00 148.00 145.00 179.00 116.00 186.00 157.00 126.00 133.00 136.00 120.00 117.00 169.00 133.00 160.00 206.00 168.00 Covance 6329-183 3M T-6295 PAGE: 60 WEEK 68 WEEK 72 143.00 156.00 157.00 96.00 179.00 137.00 139.00 164.00 136.00 136.00 149.00 149.00 173.00 155.00 196.00 144.00 142.00 116.00 152.00 128.00 127.00 195.00 130.00 165.00 180.00 125.00 133.00 141.00 150.00 124.00 SPILLED 147.00 137.00 172.00 154.00 136.00 148.00 144.00 NOT TAKEN 129.00 197.00 137.00 122.00 117.00 144.00 120.00 126.00 178.00 130.00 149.00 194.00 32.00 1405 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 36 WEEK 40 WEEK 44 WEEK 48 WEEK 52 WEEK 56 WEEK 60 WEEK 64 GROUP: C92920 C92921 C92922 C92923 C92924 C92925 C92927 C92928 C92929 C92930 GROUP: C92931 C92932 C92933 C92934 C92935 C92936 C92937 C92938 C92939 C92940 C92942 C92943 C92946 C92947 C92948 C92951 C92952 C92953 FEMALE 1 - 0 PPM 131.00 151.00 149.00 127.00 132.00 150.00 148.00 152.00 116.00 174.00 137.00 155.00 158.00 133.00 118.00 145.00 166.00 165.00 127.00 188.00 FEMALE 2 - 0.5 PPM 151.00 75.00 165.00 123.00 119.00 114.00 158.00 142.00 135.00 118.00 153.00 151.00 129.00 119.00 147.00 120.00 135.00 104.00 171.00 SPILLED 162.00 137.00 143.00 108.00 152.00 125.00 139.00 133.00 151.00 150.00 127.00 118.00 150.00 135.00 SPILLED 130.00 137.00 127.00 155.00 128.00 136.00 156.00 SPILLED 161.00 131.00 188.00 150.00 SPILLED 122.00 136.00 113.00 145.00 139.00 126.00 131.00 SPILLED 137.00 124.00 133.00 147.00 120.00 122.00 107.00 102.00 56.00 135.00 127.00 177.00 154.00 145.00 145.00 50.00 175.00 124.00 16.00 147.00 107.00 133.00 152.00 140.00 161.00 SPILLED 136.00 139.00 SPILLED 155.00 164.00 152.00 165.00 180.00 129.00 162.00 155.00 148.00 163.00 NOT TAKEN 125.00 150.00 171.00 144.00 154.00 149.00 150.00 122.00 131.00 109.00 146.00 141.00 122.00 130.00 176.00 140.00 108.00 107.00 138.00 106.00 124.00 116.00 169.00 161.00 140.00 140.00 115.00 156.00 153.00 128.00 137.00 149.00 107.00 128.00 155.00 115.00 123.00 100.00 180.00 160.00 151.00 149.00 127.00 69.00 155.00 138.00 145.00 172.00 103.00 129.00 173.00 221.00 134.00 107.00 196.00 186.00 137.00 160.00 115.00 120.00 151.00 126.00 117.00 141.00 106.00 140.00 140.00 122.00 130.00 115.00 187.00 161.00 95.00 167.00 129.00 72.00 169.00 149.00 145.00 167.00 139.00 131.00 154.00 160.00 TECH SPILL 114.00 Covance 6329-183 3M T-6295 PAGE: 61 WEEK 68 WEEK 72 143.00 NOT TAKEN 179.00 190.00 148.00 170.00 174.00 159.00 144.00 149.00 160.00 184.00 140.00 177.00 141.00 164.00 147.00 160.00 184.00 141.00 142.00 165.00 128.00 116.00 151.00 143.00 173.00 132.00 153.00 SPILLED 175.00 135.00 136.00 141.00 135.00 114.00 1406 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 36 WEEK 40 WEEK 44 WEEK 48 WEEK 52 WEEK 56 WEEK 60 WEEK 64 GROUP: C92954 C92955 C92956 C92957 C92958 C92959 C92960 C92961 C92963 C92964 C92965 C92966 C92968 C92970 C92971 C92972 C92973 C92974 C92975 C92976 C92977 C92979 C92980 C92981 C92983 C92984 C92985 C92986 C92988 C92989 C92990 FEMALE 2 - 0.5 PPM 142.00 139.00 132.00 128.00 126.00 188.00 189.00 160.00 132.00 132.00 131.00 108.00 155.00 127.00 103.00 134.00 150.00 117.00 134.00 157.00 150.00 116.00 144.00 118.00 127.00 127.00 122.00 114.00 133.00 148.00 160.00 149.00 141.00 128.00 SPILLED 122.00 157.00 140.00 153.00 123.00 SPILLED 149.00 106.00 141.00 138.00 108.00 141.00 151.00 125.00 145.00 162.00 160.00 119.00 135.00 134.00 132.00 138.00 154.00 120.00 150.00 SPILLED 162.00 149.00 131.00 150.00 143.00 122.00 SPILLED 170.00 170.00 141.00 139.00 141.00 110.00 75.00 131.00 99.00 130.00 160.00 111.00 147.00 159.00 140.00 104.00 151.00 132.00 126.00 147.00 145.00 117.00 142.00 159.00 159.00 149.00 140.00 138.00 125.00 122.00 196.00 158.00 146.00 119.00 133.00 146.00 99.00 110.00 134.00 98.00 145.00 152.00 117.00 132.00 135.00 154.00 112.00 135.00 125.00 128.00 137.00 132.00 110.00 152.00 146.00 156.00 135.00 147.00 154.00 127.00 122.00 SPILLED 159.00 170.00 136.00 127.00 158.00 101.00 120.00 144.00 102.00 144.00 152.00 119.00 139.00 173.00 151.00 99.00 140.00 129.00 105.00 141.00 115.00 109.00 135.00 SPILLED 106.00 156.00 164.00 161.00 155.00 136.00 162.00 152.00 184.00 119.00 133.00 157.00 109.00 98.00 146.00 112.00 157.00 138.00 132.00 142.00 168.00 158.00 133.00 113.00 142.00 141.00 140.00 150.00 119.00 127.00 156.00 149.00 167.00 161.00 146.00 137.00 118.00 61.00 146.00 178.00 141.00 136.00 151.00 114.00 142.00 150.00 104.00 123.00 173.00 123.00 141.00 123.00 151.00 131.00 132.00 143.00 133.00 117.00 140.00 111.00 139.00 154.00 139.00 181.00 165.00 153.00 149.00 115.00 159.00 167.00 176.00 121.00 SPILLED 135.00 129.00 125.00 162.00 113.00 142.00 155.00 127.00 152.00 138.00 137.00 130.00 146.00 147.00 159.00 163.00 135.00 142.00 154.00 157.00 Covance 6329-183 3M T-6295 PAGE: 62 WEEK 68 WEEK 72 165.00 170.00 158.00 139.00 121.00 138.00 164.00 189.00 137.00 152.00 140.00 76.00 NOT TAKEN 159.00 121.00 149.00 175.00 124.00 168.00 187.00 165.00 129.00 90.00 146.00 134.00 204.00 122.00 148.00 150.00 169.00 169.00 158.00 156.00 128.00 123.00 142.00 134.00 180.00 104.00 146.00 134.00 NOT TAKEN 145.00 102.00 153.00 169.00 133.00 154.00 195.00 167.00 121.00 NOT TAKEN 154.00 172.00 185.00 121.00 140.00 131.00 152.00 1407 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 36 WEEK 40 WEEK 44 WEEK 48 WEEK 52 WEEK 56 WEEK 60 WEEK 64 GROUP: C92991 C92992 C92994 C92997 C92998 C92999 C93001 C93002 C93003 C93004 C93005 C93007 C93009 C93010 C93011 C93012 C93013 C93014 C93015 C93016 C93017 C93019 C93020 C93021 C93022 C93024 C93025 C93026 C93027 C93028 C93029 FEMALE 3 - 2 PPM 141.00 162.00 109.00 120.00 130.00 134.00 149.00 132.00 134.00 110.00 155.00 119.00 SPILLED 161.00 119.00 170.00 108.00 152.00 138.00 139.00 136.00 138.00 154.00 132.00 120.00 129.00 156.00 140.00 123.00 139.00 SPILLED 133.00 151.00 142.00 122.00 38.00 144.00 166.00 137.00 155.00 151.00 127.00 121.00 148.00 164.00 123.00 163.00 123.00 155.00 140.00 131.00 135.00 139.00 167.00 152.00 151.00 138.00 164.00 143.00 131.00 149.00 SPILLED 115.00 144.00 95.00 115.00 TECH SPILL 144.00 136.00 139.00 131.00 138.00 121.00 114.00 SPILLED 115.00 129.00 165.00 113.00 152.00 145.00 129.00 146.00 SPILLED 109.00 139.00 131.00 145.00 139.00 131.00 125.00 143.00 SPILLED 119.00 143.00 120.00 125.00 136.00 157.00 146.00 118.00 142.00 134.00 120.00 133.00 126.00 127.00 175.00 114.00 143.00 137.00 146.00 156.00 149.00 149.00 119.00 146.00 157.00 128.00 140.00 146.00 156.00 130.00 164.00 127.00 122.00 140.00 142.00 152.00 148.00 127.00 142.00 125.00 137.00 138.00 128.00 171.00 121.00 140.00 162.00 125.00 151.00 127.00 136.00 110.00 148.00 150.00 133.00 121.00 154.00 SPILLED 133.00 150.00 125.00 124.00 144.00 147.00 150.00 143.00 144.00 157.00 129.00 142.00 155.00 134.00 183.00 121.00 150.00 145.00 148.00 167.00 156.00 62.00 147.00 142.00 152.00 131.00 145.00 133.00 160.00 125.00 149.00 93.00 119.00 155.00 156.00 168.00 146.00 146.00 145.00 128.00 132.00 143.00 136.00 175.00 133.00 169.00 162.00 150.00 174.00 114.00 50.00 149.00 154.00 169.00 139.00 132.00 136.00 173.00 142.00 150.00 136.00 125.00 155.00 146.00 157.00 146.00 144.00 128.00 136.00 134.00 147.00 136.00 181.00 133.00 161.00 136.00 83.00 SPILLED 38.00 156.00 150.00 134.00 137.00 136.00 161.00 SPILLED Covance 6329-183 3M T-6295 PAGE: 63 WEEK 68 WEEK 72 144.00 163.00 132.00 127.00 183.00 157.00 175.00 162.00 97.00 157.00 127.00 133.00 147.00 157.00 167.00 140.00 100.00 108.00 142.00 190.00 144.00 155.00 134.00 120.00 147.00 149.00 159.00 134.00 99.00 150.00 116.00 134.00 161.00 150.00 SPILLED 135.00 77.00 179.00 SPILLED 180.00 162.00 158.00 172.00 104.00 150.00 148.00 SPILLED 106.00 146.00 SPILLED 148.00 147.00 138.00 NOT TAKEN 1408 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 36 WEEK 40 WEEK 44 WEEK 48 WEEK 52 WEEK 56 WEEK 60 WEEK 64 GROUP: FEMALE 3 - 2 PPM C93030 C93032 C93033 C93034 C93036 C93037 C93038 C93039 C93040 C93041 C93042 C93043 C93044 C93046 C93047 C93048 C93049 C93050 139.00 135.00 166.00 124.00 137.00 120.00 126.00 132.00 130.00 151.00 155.00 152.00 138.00 146.00 146.00 CNTMD 122.00 153.00 134.00 146.00 148.00 136.00 159.00 138.00 140.00 146.00 130.00 165.00 158.00 148.00 127.00 157.00 175.00 134.00 120.00 145.00 GROUP: FEMALE 4 - 5 PPM C93052 C93053 C93054 C93055 C93056 C93057 C93059 C93060 C93061 C93062 122.00 144.00 140.00 120.00 143.00 119.00 138.00 111.00 154.00 133.00 142.00 139.00 151.00 147.00 142.00 127.00 135.00 144.00 151.00 130.00 136.00 135.00 167.00 122.00 167.00 117.00 128.00 135.00 158.00 162.00 165.00 168.00 128.00 151.00 177.00 118.00 115.00 147.00 133.00 134.00 138.00 142.00 141.00 109.00 128.00 134.00 164.00 126.00 134.00 155.00 163.00 131.00 171.00 146.00 131.00 132.00 138.00 170.00 166.00 182.00 129.00 155.00 156.00 120.00 136.00 152.00 139.00 146.00 149.00 152.00 140.00 126.00 140.00 140.00 163.00 129.00 138.00 145.00 80.00 136.00 163.00 150.00 146.00 163.00 129.00 169.00 154.00 203.00 139.00 128.00 157.00 113.00 123.00 157.00 144.00 148.00 143.00 147.00 151.00 131.00 145.00 147.00 125.00 178.00 194.00 SPILLED 135.00 160.00 190.00 142.00 127.00 159.00 138.00 153.00 SPILLED 147.00 175.00 135.00 130.00 152.00 153.00 171.00 196.00 197.00 58.00 162.00 173.00 141.00 139.00 159.00 143.00 152.00 71.00 152.00 171.00 83.00 127.00 148.00 153.00 174.00 199.00 196.00 58.00 151.00 194.00 138.00 67.00 156.00 147.00 155.00 142.00 156.00 135.00 116.00 125.00 162.00 171.00 133.00 139.00 162.00 143.00 146.00 144.00 121.00 137.00 151.00 193.00 132.00 129.00 80.00 125.00 154.00 138.00 124.00 150.00 153.00 194.00 136.00 84.00 78.00 112.00 163.00 147.00 126.00 147.00 140.00 SPILLED 133.00 Covance 6329-183 3M T-6295 PAGE: 64 WEEK 68 WEEK 72 163.00 158.00 159.00 174.00 146.00 162.00 SPILLED 175.00 178.00 SPILLED 146.00 196.00 143.00 48.00 163.00 146.00 133.00 138.00 184.00 158.00 158.00 167.00 192.00 SPILLED 133.00 212.00 133.00 143.00 72.00 142.00 163.00 159.00 142.00 145.00 167.00 210.00 132.00 NOT TAKEN NOT TAKEN 165.00 152.00 135.00 140.00 117.00 160.00 129.00 1409 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 36 WEEK 40 WEEK 44 WEEK 48 WEEK 52 WEEK 56 WEEK 60 WEEK 64 GROUP: C93063 C93065 C93066 C93068 C93069 C93072 C93073 C93074 C93076 C93077 C93078 C93079 C93080 C93081 C93082 C93083 C93086 C93087 C93088 C93089 C93090 C93091 C93092 C93093 C93094 C93095 C93097 C93098 C93099 C93100 C93101 FEMALE 4 - 5 PPM 121.00 123.00 SPILLED 132.00 145.00 132.00 132.00 130.00 144.00 141.00 137.00 131.00 SPILLED 108.00 134.00 124.00 132.00 104.00 135.00 123.00 107.00 136.00 140.00 156.00 127.00 118.00 142.00 131.00 139.00 122.00 108.00 121.00 121.00 SPILLED 139.00 155.00 125.00 140.00 137.00 143.00 138.00 130.00 137.00 SPILLED 121.00 138.00 140.00 132.00 113.00 142.00 143.00 114.00 144.00 152.00 156.00 141.00 117.00 137.00 114.00 138.00 159.00 105.00 117.00 121.00 SPILLED 132.00 158.00 126.00 131.00 73.00 152.00 150.00 135.00 162.00 SPILLED 109.00 131.00 141.00 136.00 118.00 126.00 108.00 127.00 136.00 135.00 144.00 128.00 118.00 129.00 116.00 135.00 148.00 128.00 123.00 131.00 160.00 141.00 153.00 141.00 SPILLED 179.00 133.00 142.00 132.00 155.00 140.00 128.00 141.00 135.00 125.00 106.00 135.00 142.00 118.00 147.00 138.00 164.00 127.00 116.00 131.00 110.00 140.00 153.00 132.00 114.00 131.00 SPILLED 134.00 165.00 135.00 184.00 154.00 126.00 121.00 131.00 161.00 SPILLED 125.00 120.00 139.00 127.00 108.00 156.00 134.00 112.00 147.00 139.00 141.00 132.00 114.00 SPILLED 108.00 148.00 141.00 109.00 123.00 126.00 183.00 152.00 154.00 128.00 168.00 156.00 152.00 136.00 199.00 165.00 SPILLED 157.00 131.00 139.00 131.00 118.00 165.00 148.00 123.00 150.00 147.00 154.00 142.00 116.00 127.00 127.00 151.00 155.00 113.00 121.00 133.00 149.00 135.00 154.00 125.00 158.00 151.00 146.00 115.00 138.00 137.00 133.00 137.00 143.00 134.00 138.00 111.00 164.00 134.00 134.00 157.00 134.00 152.00 132.00 117.00 133.00 148.00 151.00 163.00 119.00 116.00 139.00 SPILLED 137.00 156.00 142.00 144.00 153.00 127.00 170.00 146.00 165.00 SPILLED 121.00 147.00 140.00 150.00 115.00 169.00 130.00 142.00 159.00 SPILLED 148.00 151.00 115.00 131.00 130.00 151.00 169.00 114.00 Covance 6329-183 3M T-6295 PAGE: 65 WEEK 68 WEEK 72 135.00 135.00 SPILLED 144.00 167.00 153.00 148.00 154.00 193.00 171.00 155.00 178.00 159.00 140.00 148.00 159.00 142.00 135.00 111.00 135.00 143.00 166.00 158.00 137.00 143.00 133.00 162.00 132.00 147.00 161.00 113.00 114.00 109.00 SPILLED 151.00 148.00 149.00 54.00 143.00 175.00 169.00 153.00 155.00 SPILLED 122.00 147.00 158.00 157.00 116.00 80.00 143.00 133.00 186.00 66.00 161.00 145.00 122.00 126.00 132.00 143.00 160.00 133.00 1410 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 36 WEEK 40 WEEK 44 WEEK 48 WEEK 52 WEEK 56 WEEK 60 WEEK 64 GROUP: C93102 C93103 C93104 C93105 C93106 C93107 C93108 C93110 GROUP: C93112 C93113 C93115 C93116 C93117 C93118 C93119 C93120 C93121 C93122 C93124 C93125 C93126 C93128 C93129 C93130 C93131 C93132 C93133 C93134 FEMALE 4 - 5 PPM 161.00 173.00 174.00 130.00 117.00 106.00 107.00 183.00 160.00 179.00 150.00 136.00 118.00 122.00 109.00 201.00 FEMALE 5 - 20 PPM 127.00 119.00 124.00 126.00 155.00 114.00 108.00 120.00 95.00 145.00 105.00 112.00 117.00 106.00 131.00 126.00 135.00 131.00 108.00 128.00 131.00 137.00 129.00 131.00 161.00 124.00 120.00 131.00 103.00 164.00 111.00 122.00 128.00 125.00 143.00 147.00 130.00 125.00 162.00 123.00 167.00 157.00 168.00 137.00 134.00 108.00 99.00 200.00 SPILLED 124.00 125.00 130.00 154.00 125.00 116.00 123.00 SPILLED 160.00 130.00 123.00 121.00 126.00 142.00 127.00 137.00 124.00 SPILLED 126.00 172.00 169.00 170.00 142.00 125.00 SPILLED 107.00 184.00 144.00 120.00 126.00 134.00 SPILLED 125.00 119.00 123.00 103.00 183.00 123.00 115.00 127.00 121.00 148.00 139.00 138.00 135.00 140.00 133.00 169.00 156.00 157.00 139.00 106.00 116.00 NOT TAKEN 213.00 111.00 135.00 128.00 124.00 206.00 116.00 131.00 109.00 107.00 172.00 129.00 117.00 124.00 118.00 152.00 150.00 138.00 109.00 100.00 144.00 168.00 159.00 173.00 153.00 113.00 109.00 187.00 129.00 135.00 115.00 126.00 107.00 166.00 125.00 119.00 63.00 115.00 143.00 139.00 125.00 146.00 139.00 164.00 167.00 169.00 149.00 138.00 115.00 224.00 196.00 173.00 156.00 159.00 130.00 121.00 181.00 124.00 131.00 118.00 120.00 109.00 173.00 129.00 129.00 117.00 139.00 130.00 128.00 104.00 106.00 131.00 140.00 122.00 SPILLED 354.00 170.00 132.00 123.00 137.00 SPILLED 138.00 131.00 129.00 123.00 Covance 6329-183 3M T-6295 PAGE: 66 WEEK 68 WEEK 72 183.00 178.00 123.00 162.00 140.00 122.00 174.00 157.00 169.00 151.00 SPILLED 152.00 116.00 213.00 130.00 146.00 159.00 128.00 118.00 189.00 125.00 128.00 135.00 190.00 157.00 141.00 142.00 NOT TAKEN 139.00 131.00 142.00 138.00 82.00 215.00 128.00 119.00 130.00 161.00 145.00 123.00 129.00 1411 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 36 WEEK 40 WEEK 44 WEEK 48 WEEK 52 WEEK 56 WEEK 60 WEEK 64 GROUP: C93135 C93136 C93137 C93138 C93139 C93140 C93141 C93145 C93146 C93147 C93148 C93149 C93150 C93151 C93152 C93153 C93154 C93156 C93157 C93158 C93160 C93161 C93162 C93163 C93164 C93165 C93166 C93167 C93168 C93170 C93171 FEMALE 5 - 20 PPM 141.00 141.00 128.00 109.00 130.00 161.00 140.00 SPILLED 90.00 106.00 146.00 112.00 147.00 126.00 126.00 121.00 134.00 119.00 124.00 127.00 175.00 146.00 124.00 118.00 106.00 124.00 108.00 120.00 111.00 101.00 132.00 152.00 152.00 125.00 119.00 121.00 166.00 146.00 SPILLED 114.00 114.00 152.00 135.00 141.00 156.00 132.00 133.00 135.00 115.00 137.00 177.00 191.00 124.00 133.00 124.00 116.00 119.00 156.00 124.00 SPILLED 210.00 140.00 146.00 129.00 107.00 116.00 146.00 138.00 SPILLED 102.00 113.00 150.00 125.00 142.00 115.00 133.00 135.00 120.00 114.00 134.00 135.00 195.00 139.00 96.00 107.00 125.00 114.00 101.00 111.00 SPILLED 133.00 147.00 152.00 140.00 118.00 124.00 156.00 134.00 146.00 137.00 106.00 157.00 129.00 149.00 114.00 144.00 141.00 133.00 116.00 123.00 136.00 SPILLED 152.00 124.00 118.00 126.00 119.00 109.00 118.00 209.00 131.00 145.00 159.00 125.00 138.00 118.00 170.00 148.00 SPILLED 110.00 118.00 153.00 127.00 151.00 120.00 162.00 145.00 116.00 109.00 131.00 150.00 SPILLED 154.00 130.00 110.00 132.00 122.00 150.00 136.00 159.00 124.00 171.00 116.00 116.00 169.00 145.00 139.00 117.00 164.00 131.00 144.00 121.00 124.00 133.00 117.00 133.00 139.00 208.00 152.00 141.00 119.00 124.00 119.00 135.00 174.00 SPILLED 144.00 124.00 177.00 136.00 SPILLED 126.00 169.00 138.00 151.00 128.00 121.00 142.00 114.00 135.00 137.00 197.00 143.00 149.00 130.00 126.00 134.00 130.00 SPILLED 182.00 129.00 119.00 180.00 176.00 132.00 118.00 157.00 139.00 157.00 128.00 104.00 148.00 126.00 136.00 135.00 SPILLED 172.00 137.00 126.00 133.00 153.00 145.00 SPILLED Covance 6329-183 3M T-6295 PAGE: 67 WEEK 68 WEEK 72 202.00 149.00 117.00 180.00 160.00 123.00 136.00 158.00 158.00 162.00 136.00 168.00 116.00 137.00 143.00 SPILLED 136.00 149.00 141.00 136.00 135.00 142.00 SPILLED SPILLED 154.00 124.00 168.00 159.00 146.00 132.00 158.00 137.00 158.00 132.00 170.00 113.00 132.00 144.00 12.00 185.00 SPILLED 130.00 131.00 138.00 134.00 SPILLED 1412 Covance 6329-183 3M T-6295 1413 i1 i1 i1 i1 i 1 oooooooo ooooo oooooooo ooo i 1 oooooooo ooooonoooooooo ooo i H (N I W CO i H O I (4 o en LO CO CO ro Ol I--1 Ol o I--1 L-" LO CO LO o en LO Ol 0 i I iI LO ro ro i--1 I--1 I--1 i--1 I--1 i--1 i--I i--1 I--1 I--1 i--1 I--1 ro LO LO LO LO o O LO LO ro LO i--1 I--1 i--1 i--1 I--1 M I--1 i--1 I--1 i--1 Ol I--1 i--1 I--1 i--1 i--1 i--1 iI Hi (') i I I U1 < i i I I iI i I OooOoooo ooOOoOoOoo oOoooOO i I OooOoooo oooooooooonooooooo i H CO 1 w i H LO 1 LO o (N en CO CO I--1 LO CO en en o en LO en LO LO ro LO LO LO ro i 1 ro ro LO o ro ro I--1 i--1 LO LO LO LO o Ol LO LO CO LO ro LO LO i 1 I--1 i--1 i--1 i--1 i--1 i--1 I--1 i--1 I--1 I--1 i--1 I--1 i--i i--i i--1 i--i i--1 i--i M i--1 i--1 I--1 i--1 i--1 i--1 i--1 i1 i1 U1 i1 i1 i1 i1 i 1 o ooooO ooOoooOoOooOOoOo oo i 1 onnooooO ooooooooooooooOonoo i H^ 1 ww w i H LO 1 (N ro I--1 LO ro en en o ro Ol LO LO Ol L-" LO LO Ol Ol LO L-" i W1 1 1 LO i-- 1 ro C4 i-- 1 i--1 I--1 M M i--1 i--1 I--1 i--1 i--1 I--1 ro Ol Ol LO LO LO o ro LO o ro CO LO Ol LO I--1 i--1 i--i I--1 i--i I--1 i--1 i--i i--1 i--1 I--1 i--1 i--1 I--1 i--1 I--1 M i--1 Eh i 1 t < i 1 U1 U1 U1 Eh (t i 1 M1 1 & 1 M1 1 1 >H 1 1 ooooooOOo oooooooooOooOoOo OO -- 1 1 ooooooOoo oooooooooooooooonoO D LO 1 H O 1 w Eh en I H LO 1 (4 LO co I-- 1 I--1 CO CO o L-" LO L-" o L-" LO L-" en LO CO en L-" LO L-" o L-" U (N I LO 1 1 1 (N (N ro I--1 C4 I--1 o I--1 I--1 I--1 i--1 i--1 I--1 I--1 I--1 I--1 I--1 I--1 I--1 Ol ro ro ro LO ro LO ro LO LO O CO ro I--1 I--1 i--1 i--1 i--1 i--1 i--1 i--i i--1 i--1 i--i I--1 I--1 I--1 I--1 I--1 M I--1 i--1 >H 1 1 Eh Eh I 1 U1 M1 01 U - 1 1 1 M U1 1 1 O 1 1 H i 1 ai i O-- i 1 H LO 1 i H LO 1 M Eh i 1 U i 1 oO oOn w co LO ro I--1 i--1 M oooooo oooooo en o en o LO o ro ro o C4 ro i--1 i--1 i--1 I--1 I--1 i--1 oooooOoooOoooooo oooooooooooooooon ro I--1 L-" LO CO I--1 LO L-" CO CO ro ro LO w L-" o ro ro O l O l LO I--1 LO LO O l LO ro I--1 i--1 i--1 I--1 i--1 I--1 I--1 i--1 I--1 i--1 I--1 i--1 I--1 I--1 I--1 i--1 M oo oo L-" ro I--1 i--1 < i 4J < U1 i Ui 1 1 1 U1 U1 & 2i p i 1 1 -H m Ti t P . ( CU T CU C0 M 5 W U1 >H < Eh Eh Ho U M XQ OH 1 1 I 1 i 1 i 1 i 1 1 1 1 H (N I H LO 1 1 1 1 1 1 ooooooooo ooooooooo LO LO o ro LO L-" LO L-" (N i--1 en i--1 C4 i--1 o O I--1 i--1 i--i i--1 I--1 i--1 I--1 I--1 I--1 ooooOooOoOooooooOoo ooooooooooooooooooo en O l I--1 CO LO L-" CO LO LO LO LO I--1 LO ro en ro en ro i--1 O l O l ro O l LO ro O l i--1 ro i--1 I--1 i--1 I--1 I--1 I--1 I--1 i--1 I--1 i--1 i--1 i--1 i--1 I--1 I--1 i--1 i--1 i--1 i--1 i--1 i-- 1 rd Eh U < U i i i 1 1 1 HU I 1 oOoooOooo ooooooOooOOOooooooo M 1 1 ooooooooo ooooooooooooooooooo O i H CO 1 O i H^ 1 T5 t M t U P X Ui i i i 1 1 1 1 1 I--1 en co o LO LO ro C4 L-" ro i--i co I--1 i--1 i--1 i--1 I--1 LO I--1 i--i I--1 I--1 i--1 i--1 i--1 I--1 I--1 LO ro en O l en LO LO I--1 CO CO LO ro en LO LO ro o en o O l i--1 ro O l O l ro ro ro ro LO O l ro i--1 O l O l O l I--1 i--1 i--1 I--1 i--1 i--1 i--1 I--1 i--1 I--1 i--1 i--1 I--1 I--1 i--1 i--1 I--1 i--1 I--1 i 1 < H i 1 Eh i 1 H S i 1 M Eh i 1 oo oOOooo ooooooooooOoooooooo U i 1 o o no o O o o o ooooooooooooooooooo O i H^ 1 O i H^ 1 w co I--1LO C4 en o LO CO L-"LO o I--1LO LO o CO ro I--1 I--1I--1en I--1 H i HO i 1 1 (N I--1 I--1i--1i--1en o ro I--1I--1M I--1i--1i--1 I--1I--1 I--1o O Ol ro ro LO LO Ol LO ro ro Ol o Ol Ol I--1I--1i--1I--1i--1i--1i--1I--1i--1I--1i--1I--1I--1I--1i--1I--1I--1i--iI-- 1 => i i i 1 1 U1 *F i 1 o i 1 HH i 1 CU i 1 i 1 oo oOOooo ooOooOooOoOoooooooo i 1 oonoooooo ooooooooooooooooooo i HO 1 i H^ 1 2 i 1 CU i 1 cu w co LO C4 en o ro (N I-- 1 (N i-- 1 M I--1 C4 I--1 o C4 I--1 I--1 I--1 i--iI--1 i--1 2 CO ro LO en LO LO Ol ro LO LO LO LO LO L-" Ol i--1 i--1 i--1 Ol Ol i--1 LO ro LO ro ro i--1 I--1 Ol Ol I--1 i--1 i--1 i--1 i--1 I--1 i--1 I--1 i--1 i--1 i--1 i--1 I--1 I--1 i--1 i--1 I--1 I--1 I--1 i1 i 1 o U1 o i 1 (N C4 i1 i1 i 1 LO i 1 i H LO 1 H i H ro i i i ic i2 i iH i i ii oO oooooo oonoooooo w (N (N o CO ro o C4 LO (N I-- 1 I-- I-- 1 1 M I--1 C4 i--1 I--1 I--1 ro I--1 I--1 i--1 I--1 I--1 i--1 U1 LO H C >: w ooOoOoOooOOooooOooo ooO ooooooooooooO ooo o CO LO o en L-" ro L-" L-" ro LO I--1 L-" LO ro Ol I--1 ro i--1 ro i--1 ro ro en ro o Ol ro ro Ol I--1 en i--1 Ol I--1 I--1 i--1 i--1 i--1 i--1 i--1 i--1 i--1 i--1 I--1 I--1 i--1 i--1 I--1 i--1 i--1 ii i i CU i iP i iO i i i CW i O i i 2M (t >: i i i 2p 1 i <! i (N ro LO LO O CO en o o O O o O o o o CO I--1 i--1 i--1 I--1 I--1 I--1 i--1 I--1 I--1 ro ro ro ro ro ro ro ro ro en en en en en en en en en UUUUUUUUU P O O I--1 Ol ro LO LO O CO en o i--1 Ol ro LO LO o CO en CO CO CO CO CO CO CO CO CO en en en en en en en en en en I--1 I--1 i--1 i--1 i--1 i--1 i--i I--1 i--1 i--i i--i i--i i--1 i--i i--i I--1 i--1 i--1 i--i ro ro ro ro ro ro ro ro ro ro ro ro ro ro ro ro ro ro ro en en en en en en en en en en en en en en en en en en en UUUUUUUUUUUUUUUUUUU Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 36 WEEK 40 WEEK 44 WEEK 48 WEEK 52 WEEK 56 WEEK 60 WEEK 64 GROUP: C93200 C93201 C93202 C93203 C93204 C93205 C93206 C93207 C93208 C93209 C93210 C93211 C93212 C93213 C93214 C93215 C93216 C93217 C93218 C93219 C93220 FEMALE 6 - 20 PPM 123.00 103.00 103.00 140.00 117.00 119.00 124.00 122.00 132.00 SPILLED 125.00 106.00 136.00 115.00 126.00 113.00 130.00 146.00 110.00 115.00 133.00 128.00 125.00 97.00 138.00 125.00 120.00 134.00 SPILLED 115.00 SPILLED 138.00 111.00 155.00 121.00 132.00 120.00 130.00 147.00 122.00 130.00 148.00 122.00 109.00 99.00 136.00 126.00 115.00 136.00 126.00 SPILLED 139.00 105.00 150.00 96.00 131.00 119.00 132.00 134.00 115.00 136.00 149.00 123.00 146.00 103.00 139.00 138.00 128.00 139.00 129.00 SPILLED 139.00 111.00 146.00 96.00 125.00 112.00 130.00 135.00 116.00 127.00 143.00 128.00 110.00 95.00 162.00 135.00 126.00 132.00 114.00 SPILLED 153.00 106.00 145.00 143.00 131.00 125.00 137.00 134.00 127.00 128.00 143.00 136.00 111.00 103.00 175.00 152.00 123.00 148.00 118.00 SPILLED SPILLED SPILLED 178.00 136.00 148.00 164.00 152.00 158.00 137.00 151.00 156.00 142.00 133.00 120.00 162.00 143.00 137.00 157.00 134.00 SPILLED 161.00 120.00 177.00 135.00 153.00 146.00 150.00 161.00 127.00 165.00 135.00 136.00 SPILLED 105.00 162.00 123.00 140.00 155.00 160.00 168.00 168.00 154.00 131.00 112.00 149.00 138.00 164.00 151.00 135.00 161.00 155.00 Covance 6329-183 3M T-6295 PAGE: 69 WEEK 68 WEEK 72 141.00 139.00 129.00 180.00 134.00 152.00 168.00 171.00 176.00 192.00 156.00 181.00 156.00 157.00 159.00 157.00 171.00 138.00 170.00 153.00 132.00 133.00 144.00 169.00 130.00 158.00 159.00 147.00 156.00 SPILLED 140.00 181.00 148.00 171.00 154.00 151.00 154.00 121.00 159.00 142.00 1414 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 76 WEEK 80 WEEK 84 WEEK 88 WEEK 92 WEEK 96 WEEK 100 WEEK 104 GROUP: C92502 C92503 C92505 C92508 C92510 C92513 C92514 C92515 C92518 C92519 C92520 C92522 C92527 C92530 C92533 C92535 C92536 C92537 C92539 C92541 C92542 C92543 C92544 C92545 C92547 C92548 C92549 C92550 C92551 C92552 C92554 C92557 MALE 1 - 0 50.00 159.00 194.00 27.00 193.00 181.00 211.00 146.00 217.00 198.00 168.00 177.00 183.00 112.00 205.00 179.00 195.00 222.00 195.00 224.00 203.00 187.00 187.00 211.00 201.00 115.00 168.00 SPILLED 187.00 208.00 168.00 199.00 PPM 131.00 189.00 200.00 185.00 220.00 176.00 231.00 NOT TAKEN 61.00 166.00 56.00 192.00 198.00 237.00 210.00 220.00 199.00 192.00 172.00 219.00 171.00 82.00 NOT TAKEN 223.00 190.00 211.00 182.00 164.00 147.00 205.00 196.00 180.00 214.00 178.00 234.00 181.00 NOT TAKEN 142.00 187.00 230.00 195.00 224.00 191.00 187.00 182.00 227.00 159.00 210.00 194.00 187.00 176.00 88.00 126.00 187.00 207.00 168.00 175.00 175.00 242.00 169.00 191.00 231.00 161.00 229.00 198.00 187.00 177.00 230.00 NOT TAKEN 225.00 188.00 181.00 184.00 19.00 155.00 195.00 196.00 187.00 SPILLED 170.00 228.00 160.00 190.00 208.00 164.00 220.00 198.00 186.00 184.00 208.00 198.00 194.00 124.00 174.00 170.00 167.00 200.00 188.00 244.00 174.00 SPILLED 160.00 172.00 163.00 SPILLED 179.00 242.00 263.00 177.00 152.00 193.00 177.00 214.00 SPILLED NOT TAKEN 21.00 219.00 203.00 188.00 SPILLED 195.00 182.00 NOT TAKEN 152.00 140.00 180.00 104.00 247.00 185.00 112.00 180.00 225.00 191.00 40.00 140.00 NOT TAKEN 169.00 NOT TAKEN Covance 6329-183 3M T-6295 PAGE: 70 1415 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 76 WEEK 80 WEEK 84 WEEK 88 WEEK 92 WEEK 96 WEEK 100 WEEK 104 GROUP: MALE 1 - 0 PPM C92558 C92559 C92560 C92563 C92565 C92567 C92568 C92569 106.00 191.00 166.00 SPILLED 204.00 190.00 184.00 174.00 NOT TAKEN NOT TAKEN 219.00 205.00 209.00 184.00 178.00 GROUP: MALE 2 - 0 .5 PPM C92571 C92572 C92573 C92576 C92577 C92578 C92579 C92580 C92581 C92582 C92583 C92587 C92588 C92589 C92590 C92591 C92592 C92594 C92595 C92597 206.00 196.00 167.00 209.00 191.00 191.00 143.00 44.00 240.00 162.00 174.00 202.00 185.00 218.00 200.00 190.00 188.00 195.00 148.00 163.00 230.00 199.00 92.00 188.00 171.00 162.00 103.00 193.00 139.00 163.00 178.00 170.00 177.00 189.00 186.00 181.00 183.00 130.00 177.00 NOT TAKEN 198.00 183.00 195.00 179.00 221.00 200.00 NOT TAKEN 207.00 197.00 71.00 108.00 229.00 169.00 178.00 105.00 192.00 179.00 202.00 180.00 202.00 194.00 NOT TAKEN 178.00 175.00 217.00 192.00 178.00 237.00 203.00 208.00 199.00 205.00 167.00 184.00 NOT TAKEN 196.00 175.00 210.00 189.00 NOT TAKEN 197.00 139.00 177.00 184.00 167.00 176.00 139.00 164.00 167.00 230.00 197.00 219.00 200.00 122.00 195.00 121.00 193.00 SPILLED 58.00 183.00 170.00 169.00 170.00 178.00 211.00 145.00 213.00 175.00 193.00 168.00 107.00 147.00 188.00 178.00 98.00 205.00 NOT TAKEN NOT TAKEN 168.00 197.00 SPILLED 158.00 83.00 NOT TAKEN 229.00 170.00 1.00 133.00 223.00 SPILLED Covance 6329-183 3M T-6295 PAGE: 71 1416 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 76 WEEK 80 WEEK 84 WE88EK WEEK 92 WEEK 96 W1E00EK WEEK 104 GROUP: MALE 2 - 0.5 PPM C92598 C92601 C92602 C92604 C92605 C92606 C92607 C92608 C92609 C92611 C92612 C92614 C92615 C92617 C92619 C92620 C92622 C92623 C92625 C92626 C92629 C92630 177.00 187.00 186.00 103.00 187.00 218291..0000 23.00 187.00 177.00 34.00 SPILLED 185.00 134.00 165.00 172.00 207.00 156.00 168.00 117081..0000 204.00 184.00 204.00 218.00 118.00 197.00 211.00 201.00 165.00 SPILLED 17648..0000 172.00 162.00 178.00 151.00 104.00 187.00 154.00 GROUP: MALE 3 - 2 PPM C92631 C92632 C92633 C92634 C92635 C92636 182.00 219052..0000 197.00 28.00 SPILLED 189.00 180.00 219072..0000 SPILLED 190.00 197.00 193.00 106.00 197.00 91.00 200.00 166.00 SPILLED 161.00 175.00 205.00 160.00 NOT TAKEN 199.00 67.00 181.00 184.00 188.00 90.00 194.00 82.00 200.00 NOT TAKEN SPILLED 50.00 178.00 188.00 164.00 SPILLED NOT TAKEN 183.00 203.00 177.00 171.00 198.00 NOT TAKEN 192.00 117.00 162.00 SPILLED 190.00 204.00 178.00 126.00 208.00 175.00 NOT TAKEN 161.00 201.00 181.00 203.00 167.00 133.00 202.00 192.00 165.00 SPILLED 193.00 181.00 219071..0000 199.00 184.00 173.00 183.00 SPILLED 201.00 SPILLED 199.00 SPILLED 205.00 186.00 219.00 SPILLED 199.00 187.00 209.00 169.00 186.00 181.00 NOT TAKEN NOT TAKEN 181.00 175.00 158.00 199.00 250.00 Covance 6329-183 3M T-6295 PAGE: 72 1417 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 76 WEEK 80 WEEK 84 WEEK 88 WEEK 92 WEEK 96 WEEK 100 WEEK 104 GROUP: MALE 3 - 2 PPM C92637 C92638 C92639 C92641 C92642 C92643 C92644 C92647 C92648 C92649 C92651 C92653 C92654 C92655 C92656 C92657 C92658 C92659 C92660 C92661 C92663 C92664 C92665 C92666 C92667 C92669 C92671 C92672 C92673 C92674 C92675 186.00 211.00 99.00 162.00 164.00 196.00 185.00 162.00 147.00 188.00 92.00 213.00 95.00 216.00 195.00 189.00 191.00 198.00 199.00 203.00 236.00 69.00 212.00 NOT TAKEN 5.00 191.00 203.00 202.00 215.00 209.00 191.00 180.00 194.00 194.00 162.00 166.00 190.00 188.00 NOT TAKEN 141.00 SPILLED 42.00 215.00 212.00 186.00 177.00 212.00 197.00 180.00 210.00 226.00 211.00 201.00 188.00 198.00 219.00 204.00 188.00 185.00 122.00 36.00 172.00 181.00 201.00 182.00 SPILLED 188.00 220.00 211.00 201.00 196.00 211.00 SPILLED 198.00 15.00 218.00 SPILLED 211.00 235.00 226.00 213.00 111.00 189.00 191.00 116.00 165.00 172.00 171.00 196.00 191.00 180.00 184.00 179.00 201.00 202.00 202.00 171.00 184.00 195.00 199.00 154.00 183.00 114.00 240.00 NOT TAKEN 223.00 213.00 123.00 89.00 210.00 180.00 172.00 SPILLED 213.00 206.00 213.00 204.00 196.00 196.00 179.00 216.00 188.00 164.00 173.00 198.00 160.00 179.00 199.00 184.00 199.00 187.00 204.00 207.00 33.00 203.00 SPILLED 202.00 225.00 214.00 130.00 194.00 SPILLED 194.00 181.00 185.00 201.00 206.00 204.00 SPILLED 199.00 225.00 211.00 81.00 219.00 83.00 194.00 188.00 148.00 201.00 105.00 192.00 SPILLED 201.00 178.00 205.00 Covance 6329-183 3M T-6295 PAGE: 73 1418 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 76 WEEK 80 WEEK 84 WEEK 88 WEEK 92 WEEK 96 WEEK 100 WEEK 104 GROUP: MALE 3 - 2 PPM C92677 C92680 C92681 C92682 C92683 C92685 C92686 C92688 C92690 199.00 143.00 182.00 197.00 203.00 132.00 238.00 SPILLED 236.00 177.00 105.00 164.00 215.00 217.00 164.00 SPILLED 233.00 GROUP: MALE 4 - 5 PPM C92691 C92693 C92694 C92695 C92696 C92698 C92699 C92700 C92702 C92704 C92705 C92706 C92707 C92708 C92709 C92711 C92712 C92716 C92717 173.00 SPILLED 190.00 174.00 173.00 216.00 184.00 158.00 191.00 187.00 193.00 231.00 175.00 173.00 150.00 170.00 210.00 SPILLED 228.00 178.00 182.00 197.00 183.00 210.00 216.00 176.00 175.00 195.00 190.00 183.00 SPILLED 177.00 155.00 131.00 187.00 169.00 SPILLED 214.00 190.00 NOT TAKEN 148.00 202.00 213.00 200.00 231.00 217.00 SPILLED 203.00 SPILLED 247.00 179.00 187.00 204.00 180.00 211.00 232.00 171.00 188.00 205.00 204.00 191.00 214.00 182.00 195.00 98.00 199.00 132.00 SPILLED 224.00 188.00 192.00 201.00 191.00 215.00 231.00 170.00 173.00 197.00 201.00 193.00 250.00 27.00 203.00 107.00 97.00 SPILLED 231.00 202.00 224.00 211.00 89.00 229.00 171.00 194.00 207.00 179.00 206.00 228.00 170.00 203.00 208.00 198.00 198.00 SPILLED 192.00 NOT TAKEN SPILLED 219.00 204.00 203.00 213.00 91.00 228.00 230.00 207.00 224.00 SPILLED 243.00 SPILLED 187.00 159.00 210.00 190.00 218.00 215.00 163.00 187.00 217.00 209.00 205.00 174.00 197.00 186.00 148.00 206.00 192.00 216.00 143.00 181.00 154.00 135.00 197.00 224.00 194.00 165.00 156.00 206.00 199.00 217.00 15.00 175.00 120.00 106.00 187.00 220.00 209.00 167.00 186.00 SPILLED 174.00 SPILLED 177.00 Covance 6329-183 3M T-6295 PAGE: 74 1419 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 76 WEEK 80 WEEK 84 WEEK 88 WEEK 92 WEEK 96 WEEK 100 WEEK 104 GROUP: MALE 4 - 5 PPM C92720 C92721 C92723 C92724 C92726 C92727 C92729 C92730 C92732 C92733 C92735 C92736 C92737 C92738 C92739 C92740 C92741 C92742 C92743 C92745 C92746 C92747 C92748 C92749 C92750 217.00 213.00 SPILLED 214.00 194.00 193.00 106.00 196.00 189.00 186.00 198.00 196.00 192.00 207.00 186.00 176.00 SPILLED SPILLED 245.00 241.00 235.00 195.00 SPILLED 179.00 198.00 173.00 NOT TAKE 197.00 192.00 183.00 191.00 185.00 191.00 194.00 190.00 192.00 217.00 188.00 201.00 SPILLED 198.00 234.00 212.00 181.00 247.00 184.00 188.00 GROUP: MALE 5 - 2 0 PPM C92753 C92756 C92757 214.00 224.00 218.00 197.00 210.00 194.00 219.00 191.00 183.00 198.00 88.00 201.00 198.00 179.00 210.00 195.00 196.00 144.00 190.00 NOT TAKEN 105.00 193.00 235.00 191.00 197.00 231.00 200.00 126.00 190.00 22.00 198.00 190.00 68.00 260.00 188.00 199.00 29.00 195.00 50.00 SPILLED 160.00 183.00 197.00 228.00 195.00 235.00 SPILLED 193.00 152.00 190.00 184.00 NOT TAKEN 197.00 179.00 210.00 195.00 189.00 207.00 197.00 211.00 SPILLED 218.00 186.00 187.00 SPILLED 189.00 198.00 202.00 229.00 86.00 201.00 228.00 NOT TAKEN 203.00 220.00 176.00 234.00 152.00 215.00 225.00 114.00 196.00 220.00 203.00 210.00 210.00 SPILLED 184.00 196.00 183.00 187.00 159.00 229.00 219.00 89.00 198.00 201.00 172.00 211.00 187.00 CNTMD 121.00 98.00 159.00 100.00 Covance 6329-183 3M T-6295 PAGE: 75 1420 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 76 WEEK 80 WEEK 84 WEEK 88 WEEK 92 WEEK 96 WEEK 100 WEEK 104 GROUP: MALE 5 - 2 0 PPM C92761 C92762 C92763 C92764 C92766 C92767 C92768 C92769 C92770 C92771 C92773 C92774 C92775 C92776 C92780 C92781 C92782 C92783 C92784 C92785 C92786 C92788 C92791 C92792 C92797 C92800 C92802 C92803 C92804 C92806 C92807 188.00 193.00 SPILLED NOT TAKEN 197.00 190.00 207.00 208.00 215.00 204.00 180.00 180.00 213.00 191.00 SPILLED 43.00 212.00 120.00 134.00 190.00 206.00 209.00 205.00 196.00 205.00 184.00 217.00 175.00 203.00 192.00 198.00 194.00 176.00 214.00 168.00 250.00 204.00 218.00 SPILLED 26.00 190.00 167.00 218.00 178.00 222.00 219.00 NOT TAKEN 202.00 201.00 193.00 199.00 189.00 190.00 171.00 215.00 169.00 199.00 196.00 138.00 176.00 182.00 229.00 181.00 254.00 206.00 215.00 SPILLED 169.00 183.00 232.00 199.00 SPILLED 206.00 204.00 201.00 215.00 214.00 209.00 223.00 179.00 223.00 178.00 222.00 180.00 102.00 200.00 193.00 219.00 165.00 297.00 203.00 169.00 219.00 176.00 196.00 217.00 202.00 232.00 215.00 188.00 205.00 211.00 199.00 190.00 184.00 165.00 204.00 176.00 209.00 203.00 179.00 192.00 68.00 176.00 228.00 163.00 213.00 207.00 188.00 184.00 209.00 206.00 238.00 210.00 194.00 209.00 203.00 204.00 186.00 38.00 158.00 209.00 184.00 200.00 185.00 207.00 185.00 181.00 50.00 197.00 215.00 187.00 183.00 191.00 198.00 188.00 254.00 167.00 206.00 208.00 210.00 186.00 171.00 223.00 175.00 202.00 157.00 252.00 170.00 167.00 140.00 99.00 201.00 199.00 166.00 190.00 125.00 236.00 SPILLED 162.00 183.00 174.00 42.00 202.00 69.00 214.00 215.00 202.00 203.00 183.00 155.00 225.00 182.00 228.00 212.00 215.00 210.00 144.00 153.00 199.00 192.00 231.00 Covance 6329-183 3M T-6295 PAGE: 76 1421 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 76 WEEK 80 WEEK 84 WEEK 88 WEEK 92 WEEK 96 WEEK 100 WEEK 104 GROUP: MALE 5 - 20 PPM C92808 C92809 C92810 C92813 C92814 C92815 C92818 C92819 175.00 186.00 220.00 224.00 142.00 SPILLED 179.00 184.00 171.00 191.00 216.00 188.00 153.00 214.00 181.00 188.00 GROUP: MALE 6 - 20 PPM C92821 C92822 C92823 C92824 C92825 C92827 C92828 C92829 C92830 C92831 C92833 C92834 C92836 C92839 C92840 C92842 C92843 C92844 C92845 C92846 198.00 98.00 187.00 84.00 263.00 192.00 196.00 220.00 139.00 148.00 217.00 202.00 196.00 249.00 164.00 201.00 212.00 165.00 174.00 155.00 190.00 76.00 198.00 215.00 229.00 206.00 194.00 204.00 130.00 192.00 202.00 187.00 205.00 SPILLED 175.00 179.00 120.00 188.00 167.00 175.00 204.00 231.00 208.00 142.00 SPILLED 189.00 203.00 187.00 197.00 165.00 257.00 201.00 SPILLED 126.00 117.00 217.00 180.00 195.00 184.00 181.00 195.00 184.00 181.00 181.00 202.00 153.00 178.00 148.00 227.00 199.00 205.00 203.00 205.00 210.00 226.00 202.00 SPILLED SPILLED 174.00 196.00 181.00 111.00 188.00 180.00 191.00 240.00 180.00 221.00 91.00 201.00 157.00 SPILLED 194.00 225.00 178.00 167.00 206.00 223.00 209.00 SPILLED 99.00 SPILLED 186.00 199.00 206.00 210.00 188.00 238.00 31.00 159.00 151.00 SPILLED 193.00 SPILLED 172.00 186.00 194.00 SPILLED 168.00 SPILLED 192.00 190.00 178.00 178.00 207.00 NOT TAKEN 146.00 252.00 180.00 219.00 175.00 173.00 158.00 249.00 189.00 NOT TAKEN 197.00 217.00 223.00 SPILLED SPILLED 175.00 71.00 173.00 209.00 217.00 98.00 122.00 184.00 155.00 Covance 6329-183 3M T-6295 PAGE: 77 1422 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 76 WEEK 80 WEEK 84 WEEK 88 WEEK 92 WEEK 96 WEEK 100 WEEK 104 GROUP: MALE 6 - 20 PPM C92847 C92848 C92850 C92851 C92853 C92854 C92856 C92857 C92858 C92860 256.00 161.00 182.00 194.00 209.00 208.00 181.00 197.00 202.00 197.00 245.00 154.00 207.00 204.00 209.00 218.00 187.00 106.00 191.00 194.00 GROUP: FEMALE 1 - 0 PPM C92863 C92866 C92867 C92868 C92869 C92870 C92871 C92872 C92873 C92874 C92875 C92877 C92878 C92879 C92881 C92882 C92883 C92884 110.00 134.00 144.00 143.00 161.00 139.00 142.00 158.00 144.00 155.00 35.00 127.00 150.00 132.00 141.00 134.00 143.00 139.00 121.00 139.00 150.00 159.00 146.00 159.00 155.00 159.00 159.00 160.00 137.00 203.00 50.00 141.00 127.00 131.00 140.00 218.00 92.00 197.00 224.00 209.00 212.00 183.00 210.00 184.00 218.00 144.00 181.00 187.00 204.00 207.00 134.00 203.00 188.00 151.00 SPILLED 159.00 180.00 203.00 221.00 194.00 58.00 193.00 89.00 SPILLED 115.00 170.00 205.00 204.00 180.00 60.00 74.00 SPILLED 132.00 205.00 177.00 155.00 160.00 SPILLED 62.00 197.00 108.00 178.00 132.00 154.00 159.00 181.00 168.00 170.00 179.00 140.00 160.00 145.00 114.00 156.00 152.00 79.00 102.00 136.00 130.00 156.00 140.00 159.00 166.00 147.00 160.00 163.00 154.00 SPILLED 155.00 139.00 97.00 144.00 141.00 153.00 SPILLED 117.00 161.00 147.00 156.00 120.00 SPILLED 147.00 141.00 SPILLED 120.00 148.00 169.00 141.00 110.00 170.00 164.00 156.00 156.00 139.00 108.00 NOT TAKEN 92.00 147.00 163.00 144.00 108.00 175.00 143.00 159.00 158.00 152.00 156.00 83.00 150.00 174.00 147.00 25.00 179.00 149.00 111.00 130.00 142.00 127.00 Covance 6329-183 3M T-6295 PAGE: 78 1423 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 76 WEEK 80 WEEK 84 WEEK 88 WEEK 92 WEEK 96 WEEK 100 WEEK 104 GROUP: C92885 C92886 C92888 C92890 C92891 C92892 C92893 C92895 C92896 C92897 C92901 C92902 C92904 C92906 C92907 C92908 C92909 C92911 C92912 C92913 C92915 C92916 C92924 C92925 C92927 C92928 GROUP: C92933 C92934 FEMALE 1 - 0 PPM 152.00 154.00 139.00 172.00 117.00 166.00 140.00 129.00 129.00 152.00 46.00 202.00 140.00 117.00 119.00 151.00 126.00 117.00 174.00 132.00 173.00 187.00 155.00 148.00 154.00 176.00 156.00 159.00 167.00 107.00 185.00 125.00 136.00 158.00 176.00 185.00 161.00 118.00 126.00 151.00 121.00 114.00 181.00 134.00 156.00 172.00 156.00 155.00 152.00 140.00 FEMALE 2 - 0.5 PPM 147.00 133.00 146.00 121.00 152.00 136.00 141.00 187.00 165.00 125.00 140.00 144.00 174.00 161.00 133.00 139.00 136.00 124.00 113.00 156.00 133.00 177.00 197.00 99.00 180.00 151.00 154.00 152.00 161.00 169.00 NOT TAKEN 116.00 143.00 186.00 NOT TAKEN 150.00 121.00 133.00 SPILLED 118.00 120.00 183.00 125.00 149.00 60.00 123.00 141.00 136.00 183.00 124.00 148.00 156.00 161.00 147.00 130.00 SPILLED 127.00 131.00 180.00 136.00 24.00 SPILLED 115.00 153.00 157.00 142.00 179.00 175.00 110.00 137.00 119.00 176.00 166.00 138.00 145.00 78.00 161.00 150.00 125.00 126.00 125.00 193.00 116.00 NOT TAKEN 153.00 SPILLED 127.00 119.00 132.00 SPILLED 129.00 112.00 147.00 51.00 135.00 122.00 129.00 151.00 145.00 140.00 94.00 102.00 134.00 75.00 116.00 SPILLED 96.00 209.00 131.00 171.00 76.00 163.00 NOT TAKEN Covance 6329-183 3M T-6295 PAGE: 79 1424 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 76 WEEK 80 WEEK 84 WEEK 88 WEEK 92 WEEK 96 WEEK 100 WEEK 104 GROUP: C92935 C92936 C92938 C92940 C92943 C92946 C92947 C92948 C92951 C92953 C92954 C92955 C92956 C92957 C92958 C92959 C92960 C92961 C92963 C92964 C92965 C92970 C92971 C92972 C92973 C92974 C92975 C92976 C92977 C92979 C92983 FEMALE 2 - 0.5 PPM 127.00 140.00 169.00 185.00 166.00 142.00 136.00 157.00 125.00 124.00 182.00 152.00 164.00 146.00 143.00 150.00 147.00 186.00 109.00 147.00 147.00 169.00 137.00 143.00 161.00 131.00 162.00 221.00 159.00 121.00 145.00 62.00 135.00 177.00 SPILLED 174.00 126.00 158.00 176.00 158.00 116.00 156.00 145.00 173.00 146.00 141.00 167.00 153.00 SPILLED 117.00 157.00 17.00 171.00 150.00 153.00 170.00 132.00 180.00 213.00 178.00 138.00 135.00 71.00 148.00 171.00 SPILLED 92.00 146.00 146.00 192.00 126.00 119.00 182.00 124.00 159.00 134.00 91.00 175.00 170.00 SPILLED 120.00 170.00 165.00 175.00 181.00 189.00 139.00 181.00 170.00 176.00 132.00 151.00 NOT TAKEN 120.00 173.00 82.00 137.00 111.00 195.00 111.00 124.00 181.00 109.00 167.00 153.00 78.00 184.00 168.00 195.00 132.00 155.00 145.00 189.00 152.00 179.00 147.00 147.00 181.00 176.00 5.00 137.00 111.00 173.00 62.00 146.00 55.00 164.00 132.00 128.00 SPILLED NOT TAKEN 165.00 126.00 189.00 SPILLED 164.00 SPILLED 147.00 143.00 59.00 174.00 138.00 174.00 148.00 169.00 156.00 123.00 200.00 76.00 146.00 19.00 141.00 122.00 174.00 152.00 SPILLED 65.00 SPILLED SPILLED 134.00 132.00 NOT TAKEN 164.00 134.00 146.00 147.00 151.00 144.00 98.00 171.00 157.00 128.00 114.00 7.00 114.00 SPILLED SPILLED 159.00 47.00 174.00 92.00 125.00 36.00 102.00 SPILLED SPILLED 140.00 142.00 SPILLED SPILLED SPILLED 55.00 113.00 93.00 165.00 53.00 Covance 6329-183 3M T-6295 PAGE: 80 1425 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 76 WEEK 80 WEEK 84 WEEK 88 WEEK 92 WEEK 96 WEEK 100 WEEK 104 GROUP: FEMALE 2 - 0.5 PPM C92984 C92985 C92986 C92988 C92989 C92990 171.00 180.00 122.00 158.00 170.00 158.00 177.00 201.00 125.00 134.00 140.00 148.00 GROUP: FEMALE 3 - 2 PPM C92991 C92992 C92994 C92997 C92999 C93001 C93002 C93003 C93005 C93007 C93009 C93010 C93011 C93012 C93013 C93014 C93015 C93016 C93017 C93022 C93024 C93025 158.00 148.00 126.00 108.00 118.00 128.00 137.00 181.00 137.00 128.00 130.00 158.00 143.00 140.00 72.00 NOT TAKEN 156.00 SPILLED 191.00 168.00 147.00 154.00 164.00 141.00 18.00 85.00 111.00 54.00 36.00 158.00 70.00 124.00 118.00 147.00 161.00 SPILLED 52.00 158.00 107.00 172.00 168.00 63.00 159.00 175.00 171.00 147.00 158.00 149.00 136. 00 144. 00 135. 00 146. 00 135. 00 147.00 NOT TAKEN 151. 00 88.00 NOT TAKEN 179.00 127.00 133.00 119.00 121.00 166.00 67.00 170.00 210.00 167.00 98.00 167.00 1 1 1 .00 SPILLED 140. 00 143. 00 98. 00 48. 00 156. 00 158. 00 130. 00 148. 00 170. 00 134.00 118.00 140.00 154.00 142.00 155.00 NOT TAKEN SPILLED 164.00 NOT TAKEN 158.00 189.00 145.00 173.00 152.00 65.00 131.00 157.00 162.00 144.00 142.00 159.00 92.00 149.00 164.00 113.00 146.00 170.00 146.00 106.00 153.00 168.00 209.00 130.00 142.00 114.00 146.00 161.00 Covance 6329-183 3M T-6295 PAGE: 81 1426 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 76 WEEK 80 WEEK 84 WEEK 88 WEEK 92 WEEK 96 WEEK 100 WEEK 104 GROUP: FEMALE 3 - 2 PPM C93026 C93027 C93028 C93030 C93032 C93034 C93036 C93039 C93040 C93041 C93042 C93043 C93046 C93047 C93048 C93050 167.00 140.00 157.00 157.00 64.00 128.00 197.00 148.00 122.00 148.00 181.00 SPILLED 160.00 214.00 142.00 152.00 132.00 153.00 134.00 145.00 NOT TAKEN 171.00 194.00 141.00 144.00 75.00 178.00 SPILLED NOT TAKEN 204.00 135.00 148.00 GROUP: FEMALE 4 - 5 PPM C93055 C93056 C93057 C93059 C93060 C93061 C93062 C93063 C93065 C93066 C93068 C93069 7.00 156.00 129.00 161.00 146.00 SPILLED 122.00 152.00 58.00 SPILLED 163.00 161.00 167.00 135.00 152.00 160.00 NOT TAKEN 136.00 135.00 104.00 157.00 141.00 147.00 132.00 114.00 98.00 157.00 125.00 148.00 135.00 134.00 176.00 200.00 134.00 165.00 158.00 143.00 158.00 152.00 132.00 140.00 128.00 138.00 155.00 178.00 149.00 24.00 92.00 135.00 139.00 NOT TAKEN 143.00 109.00 NOT TAKEN 115.00 144.00 142.00 115.00 150.00 200.00 115.00 190.00 144.00 68.00 68.00 155.00 214.00 133.00 171.00 180.00 164.00 SPILLED 213.00 135.00 75.00 154.00 103.00 205.00 149.00 155.00 141.00 164.00 167.00 131.00 139.00 126.00 143.00 149.00 202.00 101.00 85.00 167.00 131.00 141.00 150.00 SPILLED 144.00 138.00 212.00 70.00 105.00 157.00 142.00 144.00 154.00 144.00 243.00 162.00 103.00 139.00 153.00 94.00 151.00 158.00 138.00 123.00 124.00 Covance 6329-183 3M T-6295 PAGE: 82 1427 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 76 WEEK 80 WEEK 84 WEEK 88 WEEK 92 WEEK 96 WEEK 100 WEEK 104 GROUP: C93072 C93074 C93076 C93077 C93078 C93079 C93080 C93081 C93082 C93083 C93086 C93087 C93088 C93089 C93090 C93091 C93092 C93093 C93094 C93095 C93097 C93098 C93099 C93100 C93101 C93102 C93103 C93104 C93105 C93106 C93107 FEMALE 4 - 5 PPM 136.00 158.00 188.00 169.00 131.00 149.00 154.00 130.00 141.00 152.00 67.00 112.00 126.00 128.00 144.00 111.00 59.00 161.00 132.00 123.00 143.00 128.00 165.00 155.00 137.00 163.00 189.00 3.00 127.00 147.00 101.00 149.00 51.00 160.00 174.00 157.00 168.00 169.00 123.00 144.00 169.00 78.00 133.00 131.00 132.00 148.00 157.00 145.00 130.00 130.00 148.00 162.00 114.00 181.00 197.00 108.00 SPILLED 142.00 128.00 138.00 204.00 182.00 144.00 181.00 SPILLED 125.00 166.00 195.00 118.00 138.00 138.00 146.00 134.00 130.00 164.00 175.00 173.00 162.00 133.00 158.00 170.00 112.00 135.00 140.00 142.00 136.00 160.00 132.00 139.00 138.00 146.00 168.00 126.00 193.00 146.00 NOT TAKEN 56.00 145.00 130.00 147.00 129.00 102.00 137.00 123.00 116.00 156.00 118.00 189.00 170.00 SPILLED 140.00 128.00 145.00 48.00 159.00 181.00 152.00 137.00 132.00 145.00 155.00 150.00 63.00 145.00 142.00 144.00 147.00 150.00 138.00 105.00 136.00 SPILLED 165.00 127.00 96.00 145.00 140.00 157.00 199.00 169.00 SPILLED 138.00 32.00 160.00 126.00 144.00 209.00 184.00 SPILLED SPILLED 139.00 NOT TAKEN 147.00 142.00 NOT TAKEN NOT TAKEN 110.00 114.00 130.00 NOT TAKEN 125.00 SPILLED 59.00 110.00 126.00 130.00 SPILLED 133.00 133.00 154.00 156.00 219.00 160.00 199.00 140.00 161.00 92.00 12.00 125.00 144.00 224.00 138.00 Covance 6329-183 3M T-6295 PAGE: 83 1428 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 76 WEEK 80 WEEK 84 WEEK 88 WEEK 92 WEEK 96 WEEK 100 WEEK 104 GROUP: C93110 GROUP: C93113 C93115 C93118 C93119 C93121 C93122 C93124 C93125 C93128 C93129 C93131 C93132 C93133 C93135 C93137 C93139 C93140 C93141 C93146 C93148 C93149 C93150 C93151 C93153 C93156 C93157 C93158 FEMALE 4 - 5 PPM 192.00 209.00 FEMALE 5 - 20 PPM 129.00 161.00 151.00 141.00 76.00 176.00 128.00 116.00 127.00 155.00 147.00 136.00 140.00 SPILLED 152.00 124.00 176.00 151.00 141.00 158.00 133.00 156.00 131.00 166.00 44.00 143.00 114.00 163.00 155.00 121.00 162.00 219.00 126.00 124.00 148.00 161.00 123.00 129.00 129.00 SPILLED 200.00 141.00 172.00 177.00 141.00 175.00 141.00 153.00 132.00 147.00 196.00 142.00 139.00 169.00 140.00 164.00 SPILLED 155.00 223.00 123.00 127.00 149.00 168.00 163.00 140.00 134.00 SPILLED 178.00 136.00 178.00 175.00 144.00 178.00 139.00 136.00 127.00 166.00 141.00 151.00 149.00 83.00 157.00 157.00 194.00 159.00 152.00 160.00 111.00 150.00 143.00 152.00 144.00 126.00 SPILLED 146.00 SPILLED 178.00 162.00 147.00 173.00 154.00 142.00 122.00 163.00 148.00 146.00 165.00 163.00 168.00 NOT TAKEN NOT TAKEN 138.00 116.00 146.00 97.00 78.00 116.00 123.00 SPILLED 161.00 118.00 182.00 167.00 132.00 130.00 NOT TAKEN 137.00 139.00 144.00 140.00 131.00 167.00 144.00 112.00 143.00 65.00 NOT TAKEN 131.00 136.00 SPILLED 104.00 128.00 174.00 133.00 126.00 155.00 122.00 204.00 157.00 145.00 159.00 129.00 177.00 SPILLED 201.00 134.00 SPILLED 145.00 121.00 164.00 NOT TAKEN 137.00 58.00 149.00 135.00 150.00 150.00 171.00 118.00 176.00 SPILLED 2.00 134.00 92.00 119.00 135.00 127.00 106.00 94.00 142.00 SPILLED 155.00 145.00 Covance 6329-183 3M T-6295 PAGE: 84 1429 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 76 WEEK 80 20GROUP: FEMALE 5 - PPM C93160 C93161 C93162 C93164 C93165 C93166 C93168 C93170 C93172 C93173 C93174 C93175 C93176 C93177 C93178 C93179 GROUP: NOT TAKEN 184.00 141.00 159.00 138.00 139.00 136.00 SPILLED 73.00 139.00 153.00 119.00 130.00 133.00 111142..0000 135.00 137.00 147.00 139.00 125.00 137.00 196.00 125.00 164.00 110160..0000 115131..0000 119.00 FEMALE 6 - 20 PPM C93181 C93182 C93183 C93184 C93185 C93187 C93188 C93189 C93190 C93191 C93192 C93193 130.00 136.00 146.00 153.00 149.00 144.00 178.00 127.00 158.00 179.00 171.00 NOT TAKEN 128.00 159.00 164.00 157.00 148.00 172.00 176.00 130.00 154.00 186.00 143.00 WEEK 84 193.00 142.00 160.00 138.00 155.00 146.00 165.00 109.00 SPILLED 106.00 76.00 112290..0000 108.00 137.00 159.00 163.00 156.00 148.00 161.00 170.00 64.00 163.00 196.00 143.00 WE88EK WEEK 92 WEEK 96 SPILLED 139.00 156.00 118.00 126.00 178.00 115.00 164.00 143.00 113.00 NOT11T2A.0K0EN 111171..0000 202.00 140.00 175.00 137.00 142.00 113021..0000 153.00 147.00 NOT TAKEN 109.00 114.00 117.00 209.00 133.00 189.00 153.00 139.00 131.00 139.00 SPILLED 124.00 119.00 123.00 117.00 143.00 168.00 158.00 141.00 59.00 188.00 143.00 SPILLED 167.00 144.00 150.00 173.00 175.00 160.00 SPILLED 186.00 134.00 149.00 143.00 159.00 167.00 161.00 158.00 133.00 SPILLED 59.00 159.00 W1E00EK 215.00 124.00 147.00 133.00 115202..0000 97.00 150.00 133.00 104.00 95.00 133.00 152.00 141.00 171.00 155.00 84.00 SPILLED 67.00 168.00 WEEK 104 221.00 SPILLED 164.00 127.00 139.00 130.00 150.00 114280..0000 139.00 150.00 152.00 SPILLED 163.00 150.00 NOT TAKEN 116.00 154.00 132.00 Covance 6329-183 3M T-6295 PAGE: 85 1430 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 76 WEEK 80 WEEK 84 WEEK 88 WEEK 92 WEEK 96 WEEK 100 WEEK 104 GROUP: C93194 C93196 C93197 C93198 C93200 C93201 C93202 C93203 C93204 C93205 C93206 C93208 C93209 C93210 C93211 C93212 C93213 C93214 C93215 C93216 C93217 C93218 C93219 C93220 FEMALE 6 - 20 PPM 149.00 132.00 146.00 144.00 129.00 141.00 130.00 139.00 139.00 158.00 161.00 159.00 204.00 150.00 129.00 154.00 115.00 157.00 123.00 163.00 176.00 151.00 177.00 158.00 149.00 128.00 159.00 171.00 128.00 130.00 109.00 159.00 129.00 146.00 155.00 142.00 151.00 151.00 144.00 101.00 99.00 175.00 59.00 143.00 142.00 141.00 187.00 159.00 135.00 105.00 152.00 164.00 150.00 129.00 133.00 84.00 146.00 152.00 154.00 187.00 181.00 153.00 NOT TAKEN NOT TAKEN 167.00 SPILLED 162.00 196.00 139.00 185.00 165.00 189.00 NOT TAKEN SPILLED 137.00 149.00 126.00 134.00 130.00 SPILLED 141.00 158.00 171.00 173.00 131.00 167.00 158.00 167.00 128.00 191.00 151.00 NOT TAKEN SPILLED 158.00 120.00 156.00 132.00 NOT TAKEN 140.00 151.00 169.00 137.00 148.00 184.00 168.00 183.00 163.00 151.00 155.00 NOT TAKEN 122.00 147.00 162.00 29.00 SPILLED SPILLED 131.00 SPILLED 163.00 194.00 SPILLED 144.00 146.00 143.00 140.00 146.00 37.00 170.00 54.00 143.00 156.00 145.00 156.00 159.00 93.00 NOT TAKEN 172.00 30.00 73.00 164.00 192.00 150.00 171.00 206.00 154.00 Covance 6329-183 3M T-6295 PAGE: 86 1431 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 1 ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 WEEK 9 WEEK 10 GROUP: C92571 C92572 C92573 C92574 C92575 C92576 C92577 C92578 C92579 C92580 C92581 C92582 C92583 C92584 C92585 C92586 C92587 C92588 C92589 C92590 C92591 C92592 C92593 C92594 C92595 C92596 C92597 C92598 C92599 C92600 C92601 MALE 2 - 0 . 5 PPM 0 .055 0 .061 0 .056 0 .053 0 .053 0 .051 0 .060 0 .060 0 .052 0 .059 0 .054 0 .061 0 .054 SPILLED 0 .054 0 .057 0 .060 SPILLED 0 .058 0 .061 0 .052 0 .060 0 .053 0 .056 0 .053 0 .053 0 .053 0 .059 0 .051 0 .058 0 .059 0.049 0.049 0.049 0.046 CNTMD 0.044 0.050 0.047 0.045 0.049 0.044 0.050 SPILLED 0.050 CNTMD 0.046 0.047 0.045 0.048 0.050 0.043 0.049 0.045 0.047 0.045 0.044 0.044 0.048 0.044 0.046 0.049 0 .044 0 .042 SPILLED SPILLED 0 .038 0 .039 0 .044 0 .040 0 .041 0 .042 0 .039 SPILLED 0 .039 0 .045 0 .040 0 .042 0 .042 0 .041 0 .043 0 .043 0 .040 0 .042 0 .039 SPILLED 0 .044 0 .041 0 .040 0 .045 0 .039 0 .043 0 .045 0 .038 0 .041 0 .036 NOT TAKEN NOT TAKEN 0 .036 0 .039 0 .037 0 .037 0 .038 0 .036 SPILLED SPILLED 0 .041 0 .032 0 .037 0 .037 0 .037 0 .038 0 .039 0 .037 0 .038 0 .036 0 .040 0 .036 0 .038 0 .037 0 .040 0 .034 0 .039 0 .040 0 .035 0 .037 0 .037 NOT TAKEN NOT TAKEN 0 .033 0 .035 0 .035 0 .035 0 .036 0 .032 0 .034 SPILLED 0 .037 0 .035 0 .038 0 .038 0 .037 0 .036 0 .035 0 .034 0 .034 0 .032 0 .037 0 .034 0 .036 0 .039 0 .036 0 .035 0 .036 0 .034 0.035 0.035 0.035 NOT TAKEN NOT TAKEN 0.031 SPILLED SPILLED 0.032 SPILLED 0.031 0.032 SPILLED 0.035 0.033 0.034 0.034 0.034 0.033 SPILLED 0.031 0.032 0.030 0.036 0.033 0.034 0.036 0.035 SPILLED 0.034 0.032 SPILLED 0.034 SPILLED NOT TAKEN NOT TAKEN 0.029 0.035 0.035 0.032 SPILLED 0.031 0.033 SPILLED 0.033 CNTMD 0.033 0.034 SPILLED 0.032 0.026 SPILLED 0.031 0.029 0.035 0.031 0.032 0.034 0.033 SPILLED 0.033 0.030 0 .033 0 .031 0 .030 NOT TAKEN NOT TAKEN 0 .029 0 .030 0 .030 0 .030 0 .029 0 .029 0 .030 SPILLED 0 .032 0 .031 0 .031 0 .030 SPILLED 0 .029 0 .030 SPILLED 0 .029 0 .027 0 .033 0 .030 0 .031 0 .032 0 .031 0 .030 0 .030 0 .028 SPILLED 0 .031 0 .029 NOT TAKEN NOT TAKEN 0 .027 0 .027 0 .029 0 .027 0 .028 0 .027 0 .029 0 .028 0 .031 0 .027 0 .029 0 .028 SPILLED 0 .028 0 .029 0 .027 0 .027 0 .026 SPILLED 0 .028 0 .030 SPILLED 0 .029 0 .029 0 .029 0 .027 0 .032 0 .031 0 .026 NOT TAKEN NOT TAKEN 0 .026 0 .027 0 .029 0 .026 SPILLED 0 .025 0 .026 0 .028 0 .029 0 .028 0 .028 0 .027 SPILLED 0 .027 0 .028 0 .026 0 .026 0 .026 0 .030 0 .027 0 .029 0 .030 0 .028 0 .027 0 .027 0 .025 1432 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 2 ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 WEEK 9 WEEK 10 GROUP: C92602 C92603 C92604 C92605 C92606 C92607 C92608 C92609 C92610 C92611 C92612 C92613 C92614 C92615 C92616 C92617 C92618 C92619 C92620 C92621 C92622 C92623 C92624 C92625 C92626 C92627 C92628 C92629 C92630 MALE 2 - 0 . 5 PPM 0.059 0.055 0.052 0.051 0.057 0.063 0.055 0.057 0.054 0.054 0.057 0.062 0.055 0.065 0.060 0.058 0.062 0.057 0.056 0.055 CNTMD 0.054 CNTMD 0.061 0.061 0.056 0.053 0.064 0.059 0 .047 SPILLED 0 .046 0 .042 0 .048 0 .052 0 .045 0 .047 0 .045 0 .045 0 .046 0 .052 0 .047 0 .050 0 .048 0 .048 0 .055 SPILLED 0 .050 0 .046 0 .050 0 .046 0 .039 SPILLED 0 .048 0 .047 0 .045 0 .052 0 .047 0.043 SPILLED 0.041 0.039 0.046 0.046 0.043 0.042 SPILLED 0.040 0.042 0.044 0.042 0.046 0.044 0.043 SPILLED 0.046 0.046 0.042 0.042 0.040 0.038 SPILLED 0.042 0.044 0.041 0.046 0.042 0.042 SPILLED 0.037 0.035 0.039 0.041 0.038 0.038 NOT TAKEN 0.037 0.036 NOT TAKEN SPILLED 0.035 SPILLED 0.039 NOT TAKEN 0.040 SPILLED 0.039 0.039 0.039 0.035 SPILLED 0.039 SPILLED 0.042 0.045 0.037 0.037 0.034 0.034 0.034 0.036 0.037 SPILLED 0.035 NOT TAKEN 0.034 0.033 NOT TAKEN 0.039 0.036 SPILLED 0.036 NOT TAKEN 0.036 0.036 0.039 0.035 0.036 0.034 SPILLED 0.034 0.041 SPILLED 0.040 0.035 0.034 SPILLED 0.033 SPILLED 0.034 0.034 SPILLED 0.033 NOT TAKEN 0.032 0.030 NOT TAKEN 0.037 0.034 0.034 0.033 NOT TAKEN 0.033 0.034 0.035 0.033 0.034 0.031 SPILLED 0.031 SPILLED 0.036 SPILLED 0.033 0.033 SPILLED 0.032 0.030 0.032 SPILLED SPILLED 0.032 NOT TAKEN 0.030 0.029 NOT TAKEN 0.036 0.032 0.033 0.032 NOT TAKEN 0.033 0.032 0.033 0.030 0.032 0.031 SPILLED 0.032 SPILLED 0.033 0.034 0.035 SPILLED SPILLED 0.030 0.028 0.032 SPILLED SPILLED 0.029 NOT TAKEN 0.028 0.029 NOT TAKEN SPILLED 0.031 0.032 0.029 NOT TAKEN 0.031 0.032 0.033 0.029 0.028 0.029 SPILLED 0.031 SPILLED 0.032 0.033 0.036 0.032 0.029 0.029 0.028 0.031 0.030 0.030 0.028 NOT TAKEN 0.027 0.027 NOT TAKEN SPILLED 0.029 SPILLED 0.028 NOT TAKEN 0.030 0.029 0.031 0.028 0.028 0.029 0.029 0.029 SPILLED SPILLED 0.031 0.032 0.029 0.028 0.027 0.026 0.029 0.028 0.029 0.026 NOT TAKEN 0.026 0.026 NOT TAKEN SPILLED 0.027 0.031 0.025 NOT TAKEN 0.028 0.025 0.029 0.026 0.028 0.028 SPILLED 0.028 SPILLED SPILLED SPILLED 0.031 1433 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 3 ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 WEEK 9 WEEK 10 GROUP: MALE 3 - 2 PPM C92631 C92632 C92633 C92634 C92635 C92636 C92637 C92638 C92639 C92640 C92641 C92642 C92643 C92644 C92645 C92646 C92647 C92648 C92649 C92650 C92651 C92652 C92653 C92654 C92655 C92656 C92657 C92658 C92659 C92660 C92661 0.223 0.215 0.237 0.246 0.259 0.248 0.202 0.229 0.222 0.207 0.214 0.211 0.246 0.227 0.233 0.222 0.200 0.219 0.222 0.245 0.221 0.246 0.224 0.240 0.254 0.227 0.229 0.209 0.219 0.219 0.202 0.188 0.174 0.195 0.207 0.214 SPILLED 0.174 0.193 0.186 0.183 0.193 0.180 0.196 0.181 0.186 0.181 0.179 0.190 0.195 0.207 0.189 0.207 0.187 0.207 0.212 0.185 0.207 0.178 0.188 0.172 0.171 0.164 0.154 SPILLED 0.183 0.183 0.173 0.160 0.180 0.166 0.160 0.166 0.165 0.171 0.157 SPILLED 0.156 0.160 0.171 0.173 0.176 0.166 0.181 0.163 0.187 0.190 0.162 0.169 0.167 0.165 0.174 0.155 0.150 0.129 0.145 SPILLED SPILLED SPILLED 0.152 0.165 0.149 0.147 0.159 0.153 0.151 0.142 0.155 NOT TAKEN 0.142 0.151 0.160 NOT TAKEN 0.148 NOT TAKEN 0.148 0.163 0.169 0.151 0.144 0.170 0.156 0.177 0.139 0.138 0.133 0.147 0.151 0.146 SPILLED 0.132 0.151 0.143 0.132 0.140 0.140 0.135 0.129 0.141 NOT TAKEN 0.132 0.136 0.148 NOT TAKEN 0.137 NOT TAKEN 0.131 SPILLED 0.162 0.135 SPILLED 0.136 0.139 0.132 0.126 0.131 0.124 0.140 0.139 0.142 0.143 0.126 0.141 0.137 0.127 0.131 0.135 0.128 0.125 0.135 NOT TAKEN 0.127 0.131 0.143 NOT TAKEN 0.123 NOT TAKEN 0.122 SPILLED 0.151 0.123 SPILLED SPILLED 0.134 0.138 0.119 0.124 0.117 0.131 0.131 SPILLED 0.143 0.118 0.134 SPILLED 0.128 0.119 0.121 0.119 0.113 0.123 NOT TAKEN 0.120 0.119 0.136 NOT TAKEN 0.119 NOT TAKEN 0.116 SPILLED SPILLED 0.119 0.130 0.123 0.130 0.121 0.113 0.124 0.113 0.124 0.126 0.122 0.148 0.119 0.128 0.123 0.127 0.118 0.115 0.116 0.114 0.121 NOT TAKEN 0.117 0.119 0.126 NOT TAKEN 0.115 NOT TAKEN 0.115 0.127 SPILLED 0.112 SPILLED 0.121 0.122 0.125 0.114 0.110 0.102 0.117 0.116 0.116 SPILLED 0.106 0.119 0.108 0.116 0.112 0.109 0.113 0.112 0.116 NOT TAKEN 0.109 0.111 0.120 NOT TAKEN 0.112 NOT TAKEN 0.112 0.116 SPILLED 0.104 0.116 0.109 0.117 0.113 0.098 0.110 0.102 0.113 0.120 SPILLED SPILLED 0.108 0.115 0.108 0.120 0.111 0.107 0.111 0.114 0.120 NOT TAKEN 0.107 0.116 0.124 NOT TAKEN 0.108 NOT TAKEN 0.105 SPILLED SPILLED 0.105 0.116 0.114 0.119 SPILLED 0.103 1434 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 4 ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 WEEK 9 WEEK 10 GROUP: MALE 3 - 2 PPM C92662 C92663 C92664 C92665 C92666 C92667 C92668 C92669 C92670 C92671 C92672 C92673 C92674 C92675 C92676 C92677 C92678 C92679 C92680 C92681 C92682 C92683 C92684 C92685 C92686 C92687 C92688 C92689 C92690 0.225 0.255 0.208 0.238 0.221 0.225 0.214 0.226 0.239 0.254 0.227 0.218 0.242 0.232 0.219 0.213 0.244 0.230 0.202 0.227 0.217 0.231 0.222 0.228 0.208 0.207 0.219 0.239 0.232 0.196 0.219 0.187 0.194 0.185 0.195 0.185 0.192 0.197 0.209 0.185 0.171 0.193 0.190 0.178 0.179 0.206 0.189 0.185 SPILLED 0.187 0.185 0.178 0.183 0.171 0.168 0.181 0.194 0.194 0.191 0.191 0.162 0.173 0.169 0.182 0.168 0.177 0.171 0.187 0.164 0.152 0.165 0.168 SPILLED 0.155 0.177 0.164 0.165 0.163 0.163 0.170 0.161 0.169 0.157 0.157 0.167 0.169 SPILLED SPILLED 0.175 SPILLED 0.156 0.153 0.162 NOT TAKEN 0.161 0.152 0.163 0.146 0.136 0.160 0.147 0.129 0.137 NOT TAKEN 0.146 0.145 SPILLED 0.137 0.146 0.141 0.151 0.130 0.147 0.156 0.146 0.151 0.144 SPILLED 0.139 0.142 0.141 0.147 NOT TAKEN 0.146 0.142 0.147 0.129 0.131 0.138 0.135 0.147 0.128 NOT TAKEN 0.149 0.140 0.136 0.134 0.138 0.143 0.140 0.133 0.143 SPILLED 0.136 0.131 0.133 SPILLED 0.132 0.134 0.130 0.139 NOT TAKEN 0.139 0.134 0.140 0.134 0.121 SPILLED 0.128 0.128 SPILLED NOT TAKEN 0.128 SPILLED SPILLED 0.126 0.132 0.132 0.127 0.126 0.133 SPILLED 0.131 0.117 0.124 SPILLED 0.132 0.125 0.127 0.133 NOT TAKEN 0.133 0.126 0.108 0.128 0.118 SPILLED 0.123 0.123 0.103 NOT TAKEN 0.121 0.116 SPILLED 0.118 0.121 0.123 0.124 0.120 0.124 SPILLED 0.120 0.071 0.123 SPILLED 0.120 0.123 0.123 0.130 NOT TAKEN 0.134 0.122 0.131 0.123 0.111 SPILLED 0.118 0.119 0.110 NOT TAKEN 0.122 SPILLED SPILLED 0.119 SPILLED 0.123 0.121 0.113 0.121 SPILLED 0.111 0.117 0.114 0.117 0.114 0.117 0.118 0.118 NOT TAKEN 0.128 0.111 0.121 0.114 0.107 0.131 0.118 0.109 0.109 NOT TAKEN 0.116 0.116 SPILLED 0.109 0.110 0.115 0.113 0.107 0.114 SPILLED 0.101 0.108 0.115 SPILLED 0.111 0.112 0.118 0.116 NOT TAKEN 0.125 0.117 0.122 0.118 SPILLED 0.133 0.116 0.107 0.108 NOT TAKEN 0.114 0.116 SPILLED 0.112 0.110 0.115 0.113 0.107 0.112 SPILLED 0.103 0.106 1435 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 5 ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 WEEK 9 WEEK 10 GROUP: C92691 C92692 C92693 C92694 C92695 C92696 C92697 C92698 C92699 C92700 C92701 C92702 C92703 C92704 C92705 C92706 C92707 C92708 C92709 C92710 C92711 C92712 C92713 C92714 C92715 C92716 C92717 C92718 C92719 C92720 C92721 MALE 4 - 5 PPM 0.610 0.613 0.622 0.526 0.563 0.510 0.561 0.582 0.562 0.553 0.500 0.617 0.609 0.543 0.553 0.576 0.512 0.591 0.554 0.596 0.536 0.579 0.572 0.611 0.542 0.533 0.630 0.637 0.656 0.654 0.545 0.477 0.472 SPILLED 0.409 0.450 0.427 0.467 0.448 0.458 0.471 0.421 0.491 0.513 0.455 0.445 0.474 0.446 0.477 0.466 0.502 0.433 0.465 SPILLED SPILLED SPILLED 0.439 0.496 0.526 0.525 SPILLED 0.476 0.421 0.433 0.437 0.368 0.393 0.387 0.426 0.416 0.417 0.406 0.389 0.424 0.463 0.409 0.414 0.404 0.385 0.412 0.402 0.435 0.399 0.396 SPILLED 0.449 0.399 0.393 0.434 0.478 0.439 SPILLED 0.416 0.371 0.360 0.400 0.332 SPILLED SPILLED 0.371 0.368 0.371 0.371 0.331 0.374 0.410 0.369 0.380 0.359 0.333 0.344 0.347 0.390 0.344 0.350 SPILLED 0.363 NOT TAKEN 0.322 0.366 NOT TAKEN 0.382 SPILLED 0.319 0.348 0.337 0.377 SPILLED 0.341 SPILLED SPILLED 0.346 0.340 0.330 0.316 0.349 0.371 0.344 0.359 0.327 0.309 0.299 0.361 SPILLED 0.336 0.343 0.292 0.351 NOT TAKEN 0.335 0.333 NOT TAKEN 0.356 SPILLED 0.328 0.344 0.333 0.349 0.303 SPILLED SPILLED 0.340 0.312 0.330 0.313 0.292 0.312 0.355 0.322 0.325 0.306 0.281 0.292 0.334 0.341 0.308 0.323 SPILLED 0.327 NOT TAKEN 0.317 0.323 NOT TAKEN 0.334 SPILLED 0.334 0.328 0.303 0.323 0.294 0.308 0.321 0.320 SPILLED 0.285 0.296 0.286 0.301 0.334 0.309 0.328 0.283 0.273 0.294 0.325 SPILLED 0.303 SPILLED SPILLED SPILLED NOT TAKEN 0.303 0.313 NOT TAKEN 0.317 SPILLED 0.321 0.295 0.285 0.297 0.278 SPILLED SPILLED SPILLED 0.290 0.293 0.304 0.268 0.280 0.318 0.294 0.307 0.273 0.266 0.289 0.294 SPILLED 0.276 0.286 SPILLED SPILLED NOT TAKEN 0.279 0.294 NOT TAKEN 0.300 SPILLED 0.307 0.293 0.273 0.289 0.270 0.260 SPILLED 0.319 0.281 0.283 0.289 0.267 0.279 0.304 0.287 0.307 0.270 0.271 0.289 0.278 SPILLED 0.266 SPILLED SPILLED 0.306 NOT TAKEN 0.268 0.293 NOT TAKEN SPILLED SPILLED 0.304 0.278 0.263 0.282 0.255 0.261 SPILLED 0.286 0.268 0.286 0.275 0.240 0.258 0.292 0.264 0.298 0.249 0.242 0.271 0.261 0.283 0.249 0.274 0.265 0.287 NOT TAKEN 0.253 0.264 NOT TAKEN 0.289 SPILLED 0.267 1436 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 6 ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 WEEK 9 WEEK 10 GROUP: MALE 4 - 5 PPM C92722 C92723 C92724 C92725 C92726 C92727 C92728 C92729 C92730 C92731 C92732 C92733 C92734 C92735 C92736 C92737 C92738 C92739 C92740 C92741 C92742 C92743 C92744 C92745 C92746 C92747 C92748 C92749 C92750 0.562 0.571 0.611 0.591 0.576 0.520 0.599 0.523 0.605 SPILLED 0.645 0.562 0.580 0.504 0.555 0.510 0.590 0.543 0.601 0.495 0.594 0.612 0.595 0.540 0.541 0.540 0.514 0.590 0.546 0.463 0.513 0.517 0.495 0.487 0.457 0.484 0.459 0.503 SPILLED 0.539 0.464 0.479 0.433 0.459 0.431 0.496 0.446 0.490 0.421 0.491 0.510 0.533 0.470 0.475 0.450 0.425 0.471 0.468 0.410 SPILLED 0.438 0.422 0.432 0.413 0.454 0.428 0.461 0.383 0.484 0.419 0.420 0.381 0.420 0.402 0.462 0.412 0.422 0.373 0.432 0.434 0.472 0.426 0.405 0.414 0.388 0.421 0.441 0.365 SPILLED 0.392 0.343 0.373 SPILLED 0.383 0.369 SPILLED NOT TAKEN 0.379 0.356 NOT TAKEN 0.349 0.362 0.345 0.381 0.374 0.370 0.351 0.370 0.390 NOT TAKEN 0.384 0.335 0.378 0.351 0.373 0.380 0.351 SPILLED 0.368 0.368 0.353 0.357 0.353 0.348 0.373 NOT TAKEN 0.389 0.337 NOT TAKEN 0.333 0.345 0.333 0.370 0.359 0.342 0.321 0.344 0.362 NOT TAKEN 0.362 0.342 0.365 0.342 0.352 0.353 0.315 SPILLED 0.354 0.327 0.322 0.335 0.336 0.312 0.348 NOT TAKEN 0.345 0.309 NOT TAKEN 0.312 0.324 0.305 0.339 0.333 0.321 0.303 0.316 0.329 NOT TAKEN 0.340 SPILLED 0.349 0.331 0.343 0.322 0.305 SPILLED SPILLED 0.302 0.310 0.316 0.312 0.296 0.331 NOT TAKEN 0.333 0.293 NOT TAKEN 0.302 0.306 0.290 0.330 0.324 0.323 0.317 0.296 0.315 NOT TAKEN 0.321 SPILLED 0.327 0.349 0.325 SPILLED 0.294 0.305 0.302 0.288 0.296 SPILLED 0.286 0.281 0.296 NOT TAKEN 0.311 0.294 NOT TAKEN 0.282 0.290 0.277 0.301 0.307 0.300 SPILLED 0.285 SPILLED NOT TAKEN 0.299 SPILLED 0.317 SPILLED 0.300 0.301 0.297 SPILLED 0.303 SPILLED 0.279 0.288 0.268 0.283 0.288 NOT TAKEN 0.311 0.306 NOT TAKEN 0.280 0.298 0.287 0.303 0.326 0.289 0.268 0.274 0.287 NOT TAKEN 0.289 0.269 SPILLED 0.318 0.300 0.274 0.215 SPILLED SPILLED 0.280 0.266 0.277 0.276 0.267 0.284 NOT TAKEN 0.298 0.290 NOT TAKEN 0.263 0.277 0.267 0.291 0.304 0.260 0.257 0.261 0.276 NOT TAKEN 0.269 0.251 SPILLED SPILLED SPILLED 0.267 1437 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 7 ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 WEEK 9 WEEK 10 GROUP: MALE 5 - 2 0 PPM C92751 C92752 C92753 C92754 C92755 C92756 C92757 C92758 C92759 C92760 C92761 C92762 C92763 C92764 C92765 C92766 C92767 C92768 C92769 C92770 C92771 C92772 C92773 C92774 C92775 C92776 C92777 C92778 C92779 C92780 C92781 2.238 2.125 2.223 2.024 2.444 2.144 2.295 2.215 1.973 2.117 2.109 2.172 2.283 2.355 2.097 2.309 2.195 1.996 2.288 2.023 2.368 2.350 2.024 2.332 2.590 2.198 2.456 1.921 2.228 2.255 2.156 1.990 1.717 1.889 1.807 1.960 1.888 1.921 1.876 1.673 1.853 1.912 1.793 1.839 2.001 1.816 1.920 1.923 1.781 1.883 SPILLED 1.886 1.916 1.768 1.927 2.139 1.844 2.004 1.775 1.865 2.058 1.836 1.824 1.618 1.660 1.686 1.831 1.828 1.809 1.657 1.535 1.687 1.625 1.662 1.683 1.790 1.642 1.657 1.764 1.590 1.797 1.523 1.823 1.774 1.588 1.653 1.895 1.592 1.730 1.606 1.684 1.671 1.617 1.586 NOT TAKEN 1.526 1.492 1.514 1.638 1.688 1.424 NOT TAKEN 1.488 1.510 1.522 1.440 1.497 1.467 1.458 1.520 1.403 1.491 1.319 1.709 1.439 1.424 1.490 1.599 1.413 1.509 1.412 NOT TAKEN 1.550 1.441 1.429 NOT TAKEN 1.427 1.391 1.448 1.473 1.537 1.311 NOT TAKEN 1.369 1.357 1.384 1.377 1.509 1.372 1.373 1.345 1.330 1.401 1.283 SPILLED 1.361 1.322 1.454 1.522 1.333 1.414 1.230 NOT TAKEN 1.451 1.316 1.295 NOT TAKEN 1.248 1.291 1.298 1.425 1.440 1.259 NOT TAKEN 1.286 1.289 1.312 1.234 1.411 1.274 1.269 1.354 1.266 1.297 1.152 1.305 1.281 1.179 1.363 1.378 1.264 1.288 1.195 NOT TAKEN 1.363 1.263 SPILLED NOT TAKEN 1.282 1.327 1.268 SPILLED SPILLED 1.178 NOT TAKEN 1.274 1.194 1.199 1.232 1.345 1.276 1.222 1.273 1.157 1.273 1.163 SPILLED 1.163 1.147 1.291 1.338 1.205 1.301 1.184 NOT TAKEN 1.346 1.212 SPILLED NOT TAKEN 1.187 1.160 1.171 SPILLED SPILLED 1.088 NOT TAKEN 1.150 1.186 SPILLED 1.155 1.316 1.158 1.167 1.291 1.015 1.159 1.065 1.093 1.116 1.074 1.201 1.250 1.140 1.173 1.117 NOT TAKEN 1.276 0.921 1.086 NOT TAKEN 1.166 1.129 1.196 SPILLED 1.217 1.033 NOT TAKEN 1.112 1.114 1.068 1.115 SPILLED 1.117 1.148 1.251 1.033 1.108 1.016 1.061 1.118 1.093 1.203 1.141 1.092 1.151 1.077 NOT TAKEN 1.229 1.081 1.044 NOT TAKEN 0.982 1.121 1.112 SPILLED 1.160 0.983 NOT TAKEN 1.105 1.093 1.066 1.061 1.151 1.085 1.115 1.196 0.970 1.076 0.997 1.004 1.049 0.987 1.098 1.150 1.071 1.144 1.067 NOT TAKEN SPILLED 1.048 1438 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 8 ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 WEEK 9 WEEK 10 GROUP: MALE 5 - 2 0 PPM C92782 C92783 C92784 C92785 C92786 C92787 C92788 C92789 C92790 C92791 C92792 C92793 C92794 C92795 C92796 C92797 C92798 C92799 C92800 C92801 C92802 C92803 C92804 C92805 C92806 C92807 C92808 C92809 C92810 C92811 C92812 2.583 2.109 2.165 2.051 2.136 2.134 2.438 2.233 2.514 2.101 2.078 2.122 2.035 2.163 2.002 1.892 2.390 2.150 2.221 1.993 2.221 2.051 2.265 2.170 2.348 2.336 2.354 2.179 2.169 2.391 2.072 2.064 1.739 1.768 1.804 1.741 1.821 2.089 1.824 1.944 1.745 1.937 1.703 1.694 1.823 1.690 1.612 1.820 1.711 1.898 1.693 1.797 1.720 1.803 1.687 1.897 1.978 1.896 1.924 1.803 1.972 1.715 1.887 1.633 1.718 1.675 1.618 1.749 1.833 1.709 1.750 1.717 1.613 1.524 1.642 1.657 1.548 1.481 1.652 1.612 1.696 1.604 1.594 1.569 1.698 1.562 1.670 1.762 1.782 1.654 1.657 1.792 1.523 1.617 1.425 1.373 1.506 1.401 1.584 SPILLED 1.498 1.506 1.531 1.403 NOT TAKEN 1.371 1.480 1.433 1.361 NOT TAKEN 1.382 SPILLED 1.470 1.437 1.458 1.494 1.300 1.453 1.435 1.512 1.469 1.465 1.465 1.340 1.509 1.350 1.461 1.417 1.338 1.420 1.555 1.382 1.390 1.353 1.362 NOT TAKEN 1.308 1.363 1.340 1.265 NOT TAKEN 1.402 1.392 1.375 1.352 1.379 1.463 1.393 1.348 1.561 1.397 1.453 1.412 1.563 1.293 1.381 1.270 1.335 1.361 1.236 1.334 1.304 1.300 1.304 1.300 1.271 NOT TAKEN 1.261 1.285 1.273 1.215 NOT TAKEN 1.318 1.280 1.220 1.279 1.226 1.289 1.234 1.201 1.354 1.238 1.264 1.278 1.411 1.257 1.376 1.214 1.190 1.287 1.226 1.338 1.263 1.272 1.254 1.296 1.242 NOT TAKEN 1.237 1.252 1.155 1.180 NOT TAKEN 1.184 SPILLED 1.156 1.228 1.162 1.222 1.209 1.170 1.298 1.225 1.249 1.241 1.350 1.186 1.163 1.124 1.196 SPILLED 1.173 0.886 1.022 1.108 1.148 1.217 1.174 NOT TAKEN 1.174 1.212 1.101 1.141 NOT TAKEN 1.088 1.173 1.097 1.175 1.119 1.217 1.100 0.946 1.223 1.098 1.198 1.232 1.277 1.302 1.149 1.023 1.149 1.154 1.055 SPILLED 1.159 1.091 1.089 1.096 1.089 NOT TAKEN 1.101 1.148 1.024 1.074 NOT TAKEN 1.068 SPILLED SPILLED 1.086 1.075 1.164 1.084 0.801 1.111 1.043 1.170 1.130 1.200 0.995 1.105 1.018 1.127 1.129 1.076 SPILLED 1.090 SPILLED 1.058 1.101 1.056 NOT TAKEN 1.049 1.107 0.996 1.063 NOT TAKEN 1.058 SPILLED 0.987 1.084 1.019 1.112 1.054 0.971 1.037 1.056 1.074 1.088 1.180 1.011 1439 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 9 ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 WEEK 9 WEEK 10 GROUP: MALE 5 - 2 0 PPM C92813 C92814 C92815 C92816 C92817 C92818 C92819 C92820 2.323 2.439 2.006 2.200 2.154 2.041 2.404 SPILLED 1.870 1.983 1.600 1.816 1.814 1.663 1.939 1.912 GROUP: MALE 6 - 2 0 PPM C92821 C92822 C92823 C92824 C92825 C92826 C92827 C92828 C92829 C92830 C92831 C92832 C92833 C92834 C92835 C92836 C92837 C92838 C92839 C92840 2.391 2.367 2.310 2.097 2.453 2.215 2.096 2.214 2.323 2.562 2.323 2.396 2.411 2.232 2.409 2.079 2.353 2.455 2.592 2.134 1.883 1.905 1.865 1.695 1.997 SPILLED 1.769 1.786 1.819 2.071 1.944 1.988 1.901 1.901 1.793 1.752 1.948 1.946 2.137 1.710 1.649 1.787 1.514 1.637 1.652 1.499 1.798 1.637 1.730 1.641 1.698 1.526 1.754 1.717 1.650 1.638 1.710 1.808 1.720 1.706 1.705 1.726 1.533 1.586 1.728 1.721 SPILLED 1.512 1.493 1.534 SPILLED 1.431 1.459 1.386 1.570 1.542 1.480 1.480 1.459 1.421 1.568 1.510 1.507 1.433 1.525 1.731 1.471 1.499 1.474 1.507 1.426 1.438 1.548 1.568 1.681 1.439 1.366 1.388 1.414 1.347 1.351 1.307 1.429 1.501 1.397 1.402 1.369 1.336 1.502 1.382 1.448 1.385 1.475 1.579 1.360 1.400 1.358 1.368 1.309 1.352 1.401 1.438 1.553 1.295 1.257 1.292 1.102 1.219 1.241 1.224 1.355 1.432 1.302 1.189 1.313 1.172 1.207 1.148 1.265 1.333 1.214 1.162 1.434 1.127 1.223 1.217 1.269 1.274 1.127 1.047 1.305 0.993 1.063 1.091 1.122 1.266 1.102 1.047 1.290 1.024 1.059 1.070 1.136 SPILLED 1.312 1.361 1.222 1.274 1.404 1.289 1.340 1.286 1.385 1.431 1.268 1.266 1.205 1.279 1.290 1.233 1.258 1.322 1.368 1.219 1.211 1.249 1.150 1.218 1.302 SPILLED 1.293 1.217 1.291 1.271 1.245 SPILLED SPILLED 1.213 1.168 1.166 1.186 1.258 1.254 1.133 1.136 1.166 1.096 1.120 1.255 SPILLED 1.266 1.171 1.242 1.339 1.232 SPILLED SPILLED 1.145 1.123 0.731 1.099 1.217 SPILLED 1.065 1.071 1.114 1.081 1.162 1.178 1.221 1.154 1.137 1.175 1.296 1.139 SPILLED SPILLED 1.143 1.109 1.091 1.043 1.177 1.173 1.079 1.073 1.067 1.070 1.195 SPILLED 1.233 1.174 1.095 1.162 1.299 1.146 1.021 1.035 1.093 1.065 1.259 1.033 1.118 1.140 1.052 1440 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 10 ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 WEEK 9 WEEK 10 GROUP: C92841 C92842 C92843 C92844 C92845 C92846 C92847 C92848 C92849 C92850 C92851 C92852 C92853 C92854 C92855 C92856 C92857 C92858 C92859 C92860 MALE 6 - 2 0 PPM 2.011 2.260 2.248 2.442 SPILLED 2.491 2.519 2.611 2.521 2.122 2.213 2.393 2.159 2.416 2.489 2.719 2.228 2.214 2.121 2.519 1.822 1.830 1.892 1.892 SPILLED 1.935 2.155 2.062 2.072 1.800 1.890 1.994 1.819 2.022 SPILLED 2.028 1.864 1.762 1.848 2.070 GROUP: FEMALE 2 - 0.5 PPM C92931 C92932 C92933 C92934 C92935 C92936 C92937 C92938 0.052 SPILLED 0.051 0.053 0.054 0.053 0.050 0.050 0.049 SPILLED 0.050 0.052 0.050 0.049 0.050 SPILLED 1.575 1.616 1.726 1.685 1.728 1.680 1.890 1.873 1.763 1.639 1.607 1.861 1.602 1.811 SPILLED 1.786 1.620 1.766 1.711 1.905 0.046 SPILLED 0.046 0.050 0.051 0.048 0.046 SPILLED 1.515 1.452 1.506 1.519 1.610 1.543 1.624 1.709 1.551 1.513 1.476 1.782 1.483 SPILLED SPILLED 1.527 1.486 1.531 1.523 1.652 0.042 SPILLED 0.047 SPILLED 0.046 0.040 0.043 SPILLED 1.267 1.276 1.400 1.377 1.515 1.406 1.434 1.552 1.473 1.394 1.341 1.451 1.359 1.425 1.402 1.425 1.322 1.460 1.481 1.468 0.040 SPILLED 0.043 0.043 0.042 0.042 0.042 SPILLED 1.225 1.182 1.281 1.293 1.419 1.354 1.326 1.460 1.366 SPILLED 1.223 1.297 1.287 1.297 SPILLED 1.302 1.263 1.281 1.378 1.407 0.038 SPILLED SPILLED SPILLED 0.036 0.038 0.040 SPILLED 1.141 1.160 1.190 1.264 1.331 1.288 1.243 1.361 1.322 1.338 1.190 1.323 1.198 1.246 0.943 1.213 1.176 1.251 1.316 1.309 0.036 SPILLED 0.040 0.046 0.036 0.036 0.040 SPILLED 1.114 1.096 1.133 1.204 1.248 1.219 1.219 1.312 SPILLED 1.265 1.134 1.347 1.208 1.211 SPILLED 1.163 1.157 1.172 1.201 1.237 0.036 SPILLED 0.039 0.038 0.036 0.035 SPILLED SPILLED 1.068 1.062 1.123 1.163 SPILLED 1.140 1.137 1.272 SPILLED SPILLED 1.067 SPILLED 1.120 1.106 SPILLED 1.124 1.036 1.112 SPILLED 1.255 0.033 SPILLED 0.039 0.036 0.034 0.034 0.037 SPILLED 1.044 1.039 1.088 1.108 1.236 1.149 1.134 1.260 1.251 1.231 1.050 1.252 1.057 1.134 1.200 1.106 1.022 1.081 SPILLED 1.175 0.031 SPILLED 0.038 SPILLED 0.032 0.030 SPILLED SPILLED 1441 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 11 ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 WEEK 9 WEEK 10 GROUP: C92939 C92940 C92941 C92942 C92943 C92944 C92945 C92946 C92947 C92948 C92949 C92950 C92951 C92952 C92953 C92954 C92955 C92956 C92957 C92958 C92959 C92960 C92961 C92962 C92963 C92964 C92965 C92966 C92967 C92968 C92969 FEMALE 2 - 0.5 PPM 0.047 0.053 0.057 0.053 0.054 0.050 0.054 0.047 0.052 0.048 SPILLED 0.054 0.048 0.048 0.050 0.054 0.055 0.049 SPILLED 0.047 0.054 SPILLED SPILLED 0.048 0.046 0.049 0.059 0.049 0.056 0.056 0.048 0.046 0.051 SPILLED SPILLED 0.049 SPILLED 0.056 0.047 0.049 0.052 SPILLED 0.048 0.047 0.046 0.046 0.053 0.049 0.046 0.050 0.050 0.052 0.061 SPILLED 0.048 0.045 0.067 0.064 0.045 0.052 SPILLED 0.051 0.056 0.051 SPILLED SPILLED 0.049 0.045 0.051 0.044 0.048 0.045 0.054 0.050 SPILLED 0.047 SPILLED 0.046 0.049 0.047 0.048 0.043 0.049 SPILLED SPILLED 0.046 0.043 0.048 SPILLED 0.041 0.050 0.050 SPILLED 0.043 0.053 NOT TAKEN SPILLED 0.044 0.045 NOT TAKEN 0.039 0.044 0.040 0.046 NOT TAKEN 0.041 0.043 0.038 0.044 SPILLED 0.041 0.041 0.045 0.036 SPILLED 0.040 0.038 0.041 SPILLED SPILLED 0.040 0.047 SPILLED NOT TAKEN 0.045 0.048 NOT TAKEN SPILLED 0.043 0.043 NOT TAKEN 0.040 0.045 0.043 0.047 NOT TAKEN 0.045 0.043 0.043 0.046 SPILLED 0.039 0.042 0.043 0.046 0.048 0.037 0.039 0.039 SPILLED 0.045 0.040 0.043 0.046 NOT TAKEN SPILLED SPILLED NOT TAKEN SPILLED SPILLED 0.038 NOT TAKEN 0.040 0.040 0.036 0.046 NOT TAKEN 0.037 0.037 0.040 0.039 SPILLED 0.038 SPILLED 0.037 0.040 SPILLED 0.034 0.036 0.036 SPILLED SPILLED 0.035 0.045 SPILLED NOT TAKEN SPILLED SPILLED NOT TAKEN 0.041 0.039 0.037 NOT TAKEN 0.036 0.038 0.038 0.039 NOT TAKEN 0.038 0.038 0.037 0.039 0.038 0.036 0.042 0.038 0.040 0.040 0.034 0.035 0.036 SPILLED 0.041 0.037 0.037 0.037 NOT TAKEN SPILLED SPILLED NOT TAKEN SPILLED SPILLED 0.038 NOT TAKEN 0.034 0.039 0.038 SPILLED NOT TAKEN 0.038 0.037 SPILLED 0.037 0.037 0.033 SPILLED 0.038 0.041 SPILLED 0.031 0.036 0.038 SPILLED SPILLED 0.035 SPILLED 0.035 NOT TAKEN 0.038 SPILLED NOT TAKEN 0.036 0.035 0.035 NOT TAKEN 0.033 0.035 0.032 0.033 NOT TAKEN 0.037 0.034 0.035 0.035 0.036 0.032 SPILLED 0.034 0.040 0.034 0.030 0.033 0.037 0.033 0.035 0.035 0.033 0.037 NOT TAKEN 0.035 SPILLED NOT TAKEN SPILLED 0.035 0.034 NOT TAKEN 0.032 0.033 0.029 0.033 NOT TAKEN 0.032 0.035 0.035 0.035 0.031 0.031 0.039 0.031 0.033 0.033 0.032 0.032 SPILLED SPILLED 0.034 0.033 0.031 0.033 NOT TAKEN 1442 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 12 ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 WEEK 9 WEEK 10 GROUP: FEMALE 2 - 0.5 PPM C92970 C92971 C92972 C92973 C92974 C92975 C92976 C92977 C92978 C92979 C92980 C92981 C92982 C92983 C92984 C92985 C92986 C92987 C92988 C92989 C92990 0.054 0.047 0.051 0.051 0.049 0.056 0.046 0.054 0.053 0.051 0.051 0.053 0.053 0.056 0.048 0.057 0.054 0.051 0.047 0.067 0.053 0.049 0.047 0.053 0.055 0.046 0.054 0.046 0.048 0.050 0.046 0.051 0.047 SPILLED SPILLED 0.048 0.051 0.054 0.051 0.051 SPILLED SPILLED GROUP: FEMALE 3 - 2 PPM C92991 C92992 C92993 C92994 C92995 C92996 C92997 SPILL0E.2D22 00..221002 0.231 0.208 0.198 SPILLED 0.183 0.188 00..210718 0.192 0.182 000000000.........000000000444444445684668971 0.045 00..004486 SPILL000E...D000444479 00000.....000004455544151 0000000.......221111120767786038641 0.046 0.040 0.045 0.044 0.040 0.043 0.043 0.043 0.042 0.044 0.045 SPILLED NOT TAKEN 0.045 0.045 0.045 0.045 0.040 0.043 TECH SPILL 0.045 0.043 0.039 0.043 0.047 0.039 0.048 0.043 0.047 0.044 0.041 0.045 0.037 NOT TAKEN SPILLED 0.039 0.042 0.044 0.039 0.045 SPILLED 0.041 SPILLED SPILLED 0.153 0.166 0.203 NOT TAKEN 0.170 SPILLED SPILLED 0.176 0.151 0.158 NOT TAKEN 0.153 0.038 0.035 0.040 0.042 0.037 0.042 0.035 0.042 0.039 0.039 0.043 SPILLED NOT TAKEN 0.044 0.039 0.042 0.043 0.041 0.042 0.063 0.045 SPILLED SPILLED 0.165 0.146 0.161 NOT TAKEN 0.138 0.038 0.037 0.039 0.049 0.037 0.044 0.034 0.039 0.040 0.037 0.038 0.036 NOT TAKEN 0.041 0.036 0.040 0.039 0.035 0.038 SPILLED 0.040 0.156 0.141 0.142 0.132 0.151 NOT TAKEN 0.143 0.039 0.033 0.040 0.041 0.034 0.037 0.033 0.036 0.035 0.033 0.039 0.033 NOT TAKEN SPILLED 0.036 0.035 0.037 0.036 0.037 SPILLED SPILLED 0.141 SPILLED 0.145 0.142 0.152 NOT TAKEN 0.151 0.035 0.034 0.035 SPILLED 0.032 0.038 0.032 0.037 0.032 0.033 0.033 0.031 NOT TAKEN SPILLED 0.032 0.031 0.037 0.033 0.036 SPILLED 0.037 0.136 0.135 0.142 0.133 NOT TAKEN NOT TAKEN 0.136 0.032 0.032 0.036 0.036 0.033 0.037 0.031 0.034 0.033 0.033 0.035 0.032 NOT TAKEN 0.033 0.030 0.032 0.036 SPILLED 0.037 SPILLED 0.037 0.134 0.136 0.132 0.124 NOT TAKEN NOT TAKEN SPILLED 1443 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 13 ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 WEEK 9 WEEK 10 GROUP: C92998 C92999 C93000 C93001 C93002 C93003 C93004 C93005 C93006 C93007 C93008 C93009 C93010 C93011 C93012 C93013 C93014 C93015 C93016 C93017 C93018 C93019 C93020 C93021 C93022 C93023 C93024 C93025 C93026 C93027 C93028 FEMALE 3 - 2 PPM 0.212 SPILLED SPILLED 0.220 0.209 0.287 0.227 0.219 0.186 SPILLED 0.221 0.230 0.241 0.226 SPILLED SPILLED SPILLED 0.211 0.164 0.200 0.208 0.207 0.221 0.222 0.195 0.174 0.207 0.210 0.201 0.191 0.188 0.211 SPILLED SPILLED 0.202 0.190 SPILLED 0.203 0.193 0.177 SPILLED 0.213 0.190 0.207 0.189 SPILLED 0.204 0.220 0.198 0.186 0.205 0.194 0.190 0.225 0.207 0.206 0.189 0.188 0.193 0.195 0.181 0.184 0.219 SPILLED 0.202 0.177 0.179 0.175 0.240 0.176 0.149 SPILLED 0.195 0.264 0.202 0.179 0.175 0.182 SPILLED 0.187 0.216 0.180 0.182 SPILLED 0.208 0.193 0.170 0.178 0.188 0.201 0.178 0.173 0.170 0.181 SPILLED SPILLED 0.196 0.151 0.185 0.174 0.177 NOT TAKEN SPILLED NOT TAKEN 0.176 0.187 0.163 0.185 SPILLED 0.214 SPILLED 0.221 0.193 SPILLED SPILLED 0.173 SPILLED 0.178 0.157 0.175 0.171 0.179 0.171 0.163 0.187 SPILLED 0.173 SPILLED 0.165 0.176 0.167 0.168 NOT TAKEN 0.173 NOT TAKEN 0.169 0.169 0.152 0.163 SPILLED 0.179 0.157 0.189 0.177 0.169 SPILLED 0.168 0.165 0.170 0.162 0.161 0.151 0.178 0.170 SPILLED 0.163 SPILLED SPILLED SPILLED 0.147 0.147 0.165 0.157 NOT TAKEN SPILLED NOT TAKEN SPILLED 0.166 SPILLED 0.141 SPILLED 0.161 SPILLED 0.168 0.187 0.157 SPILLED SPILLED 0.157 0.148 SPILLED 0.157 0.166 0.156 0.172 SPILLED 0.163 SPILLED 0.144 0.144 0.128 0.145 0.153 0.141 NOT TAKEN SPILLED NOT TAKEN 0.145 0.152 0.126 0.146 0.149 SPILLED 0.149 0.160 0.161 0.140 SPILLED 0.160 0.145 0.147 SPILLED 0.148 0.143 0.144 0.148 0.150 0.152 SPILLED SPILLED 0.154 0.149 SPILLED 0.143 0.150 NOT TAKEN SPILLED NOT TAKEN 0.147 0.150 0.133 0.127 0.145 0.133 SPILLED 0.149 0.160 0.137 0.153 SPILLED 0.140 0.137 0.139 0.144 0.151 0.143 0.146 0.141 0.145 SPILLED SPILLED 0.155 0.125 0.137 0.136 0.127 NOT TAKEN SPILLED NOT TAKEN 0.147 0.144 0.124 0.129 0.128 0.129 SPILLED 0.148 0.157 0.131 SPILLED 0.139 0.128 0.130 SPILLED 0.134 SPILLED SPILLED 0.137 0.145 0.145 SPILLED SPILLED 0.156 0.137 0.119 0.138 0.139 NOT TAKEN SPILLED NOT TAKEN SPILLED 0.145 0.116 0.121 0.145 0.133 0.152 0.146 SPILLED 0.136 SPILLED SPILLED 0.139 0.134 SPILLED 0.139 0.139 0.148 0.131 0.144 1444 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 14 ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 WEEK 9 WEEK 10 GROUP: FEMALE 3 - 2 PPM C93029 C93030 C93031 C93032 C93033 C93034 C93035 C93036 C93037 C93038 C93039 C93040 C93041 C93042 C93043 C93044 C93045 C93046 C93047 C93048 C93049 C93050 0.206 SPILLED 0.221 0.207 SPILLED 0.195 SPILLED 0.192 0.219 0.251 0.224 SPILLED 0.222 0.205 0.217 0.218 SPILLED 0.222 0.226 0.227 0.207 0.198 0.207 0.191 0.205 0.187 SPILLED 0.186 0.212 0.210 0.202 0.205 0.223 SPILLED 0.221 0.181 0.203 SPILLED 0.301 0.204 0.204 0.207 0.196 0.195 GROUP: FEMALE 4 - 5 PPM C93051 C93052 C93053 C93054 C93055 C93056 0.567 0.560 0.513 SPILLED 0.570 0.569 SPILLED SPILLED 0.457 SPILLED 0.473 SPILLED 0.180 0.189 0.216 0.187 SPILLED 0.193 0.245 SPILLED 0.190 0.227 0.227 0.289 0.222 0.178 0.186 SPILLED SPILLED SPILLED 0.176 0.189 0.174 0.201 0.554 0.491 0.462 0.493 0.461 0.442 SPILLED 0.185 NOT TAKEN 0.156 SPILLED 0.149 0.187 0.163 0.178 SPILLED 0.191 SPILLED 0.189 0.180 0.172 0.178 NOT TAKEN 0.190 0.226 0.197 0.164 0.205 SPILLED SPILLED 0.415 0.456 0.453 0.426 SPILLED 0.162 NOT TAKEN 0.168 SPILLED 0.171 0.190 0.193 0.174 SPILLED 0.183 SPILLED SPILLED 0.175 0.178 0.159 NOT TAKEN 0.154 0.184 0.175 0.166 0.188 SPILLED SPILLED 0.441 SPILLED 0.427 0.421 SPILLED 0.154 NOT TAKEN 0.153 SPILLED 0.146 0.076 0.153 0.164 SPILLED 0.171 SPILLED SPILLED 0.148 0.166 0.142 NOT TAKEN 0.200 SPILLED 0.168 0.149 0.174 SPILLED SPILLED 0.372 SPILLED 0.380 0.380 SPILLED 0.138 NOT TAKEN 0.144 SPILLED 0.125 0.151 0.148 0.150 0.159 0.155 SPILLED 0.167 0.142 0.159 0.146 NOT TAKEN SPILLED 0.150 SPILLED 0.141 0.171 0.394 SPILLED 0.408 SPILLED 0.352 0.363 SPILLED 0.150 NOT TAKEN 0.145 0.173 0.133 0.167 0.150 0.136 0.183 0.152 SPILLED 0.161 0.144 0.142 SPILLED NOT TAKEN SPILLED 0.138 0.162 SPILLED 0.169 0.412 SPILLED 0.406 SPILLED SPILLED 0.343 SPILLED 0.148 NOT TAKEN 0.132 SPILLED 0.137 SPILLED SPILLED 0.142 0.176 0.150 0.262 0.174 0.124 0.144 0.131 NOT TAKEN 0.142 0.122 0.148 0.157 SPILLED 0.368 SPILLED 0.399 SPILLED 0.371 0.363 SPILLED SPILLED NOT TAKEN 0.135 SPILLED 0.137 0.136 0.141 0.129 SPILLED 0.151 SPILLED 0.158 0.133 0.138 0.141 NOT TAKEN 0.132 0.125 0.160 0.145 SPILLED 0.478 SPILLED 0.363 SPILLED SPILLED 0.330 1445 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 15 ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 WEEK 9 WEEK 10 GROUP: C93057 C93058 C93059 C93060 C93061 C93062 C93063 C93064 C93065 C93066 C93067 C93068 C93069 C93070 C93071 C93072 C93073 C93074 C93075 C93076 C93077 C93078 C93079 C93080 C93081 C93082 C93083 C93084 C93085 C93086 C93087 FEMALE 4 - 5 PPM 0.643 0.569 0.583 0.557 0.483 0.514 0.503 0.539 0.576 0.488 0.495 0.525 0.568 0.606 0.502 0.565 0.591 0.558 0.534 0.557 0.487 0.570 0.572 0.581 0.576 SPILLED 0.565 0.528 0.547 0.597 SPILLED 0.509 0.493 0.506 0.597 0.458 0.472 0.488 0.496 0.502 0.424 0.437 SPILLED 0.605 SPILLED 0.434 0.531 0.515 0.563 0.480 0.498 0.435 0.531 0.679 0.503 0.469 SPILLED 0.488 0.472 SPILLED SPILLED 0.589 0.525 0.464 0.496 0.507 0.439 0.458 0.429 0.476 0.503 0.440 0.495 0.469 0.488 0.527 0.436 0.504 0.499 0.539 0.489 0.563 0.433 0.486 0.467 0.457 0.472 SPILLED 0.466 0.451 0.477 SPILLED 0.606 0.457 NOT TAKEN 0.432 0.422 0.379 0.457 SPILLED 0.430 0.493 0.380 NOT TAKEN 0.401 0.424 NOT TAKEN 0.397 SPILLED 0.469 0.396 NOT TAKEN 0.452 0.379 0.483 0.498 0.407 0.411 SPILLED 0.424 NOT TAKEN 0.425 0.415 0.436 0.433 NOT TAKEN 0.426 TECH SPILL 0.381 0.418 0.396 0.405 0.439 SPILLED NOT TAKEN 0.423 0.403 NOT TAKEN 0.378 SPILLED 0.479 SPILLED NOT TAKEN 0.464 0.363 0.483 0.437 0.444 0.411 SPILLED 0.498 NOT TAKEN 0.486 0.424 SPILLED 0.397 NOT TAKEN 0.374 0.389 0.374 0.380 0.342 0.372 0.421 SPILLED NOT TAKEN 0.361 0.350 NOT TAKEN 0.366 SPILLED 0.405 0.450 NOT TAKEN SPILLED 0.352 0.436 0.406 0.363 0.395 SPILLED 0.404 NOT TAKEN 0.383 0.352 0.405 0.385 NOT TAKEN 0.380 0.400 0.354 0.386 0.326 0.387 0.418 SPILLED NOT TAKEN 0.349 0.341 NOT TAKEN 0.378 SPILLED 0.412 0.394 NOT TAKEN 0.419 0.361 0.403 0.416 0.331 0.362 0.402 0.399 NOT TAKEN SPILLED 0.357 0.397 0.350 NOT TAKEN 0.352 0.355 0.352 0.371 0.314 0.400 0.412 SPILLED NOT TAKEN 0.360 0.336 NOT TAKEN 0.367 SPILLED 0.392 0.378 NOT TAKEN 0.378 0.333 0.408 0.463 0.333 0.375 SPILLED 0.383 NOT TAKEN 0.339 SPILLED SPILLED 0.368 NOT TAKEN 0.343 0.329 0.317 0.345 0.311 0.388 SPILLED 0.369 NOT TAKEN 0.356 0.340 NOT TAKEN 0.360 SPILLED 0.388 0.379 NOT TAKEN 0.378 0.308 0.399 0.328 0.337 0.340 SPILLED 0.401 NOT TAKEN 0.345 SPILLED 0.331 0.375 NOT TAKEN 0.343 0.314 SPILLED 0.332 0.336 0.368 0.383 0.341 NOT TAKEN 0.340 0.299 NOT TAKEN 0.344 0.414 0.348 0.389 NOT TAKEN 0.383 0.304 0.398 0.350 0.299 0.380 0.347 0.344 NOT TAKEN SPILLED 0.344 SPILLED 1446 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 16 ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 WEEK 9 WEEK 10 GROUP: FEMALE 4 - 5 PPM C93088 C93089 C93090 C93091 C93092 C93093 C93094 C93095 C93096 C93097 C93098 C93099 C93100 C93101 C93102 C93103 C93104 C93105 C93106 C93107 C93108 C93109 C93110 0.565 0.586 SPILLED 0.519 0.566 0.591 0.658 0.598 0.650 0.487 0.588 0.534 0.609 0.486 0.595 0.537 0.544 0.706 0.506 0.549 0.551 0.534 SPILLED 0.477 0.484 SPILLED 0.460 0.495 0.474 0.663 0.485 SPILLED 0.467 SPILLED 0.492 0.560 SPILLED 0.528 0.528 0.517 0.527 0.467 0.483 0.508 0.477 0.602 GROUP: FEMALE 5 - 20 PPM C93111 C93112 C93113 C93114 C93115 2.224 2.139 SPILLED 2.022 1.991 2.088 1.969 SPILLED 1.937 2.001 0.459 0.459 SPILLED 0.413 0.501 0.460 0.520 0.479 0.568 0.462 0.527 0.461 0.504 0.432 0.482 0.467 0.488 0.478 0.429 SPILLED 0.449 0.453 SPILLED 1.889 1.709 SPILLED 1.749 1.837 0.416 0.569 SPILLED 0.434 SPILLED 0.431 0.654 0.461 SPILLED 0.475 SPILLED 0.410 0.489 0.412 0.442 0.408 0.463 0.414 0.404 0.444 0.425 0.433 SPILLED 1.644 1.581 1.833 NOT TAKEN 1.675 0.402 0.432 SPILLED 0.352 0.428 0.420 0.433 0.483 0.511 0.439 0.410 0.382 0.441 0.405 0.459 0.454 0.476 0.451 0.408 0.412 0.410 0.417 0.497 1.546 1.546 1.745 NOT TAKEN 1.876 0.352 SPILLED SPILLED 0.363 0.428 SPILLED 0.502 SPILLED 0.459 0.405 0.402 0.344 SPILLED 0.409 0.403 0.396 SPILLED 0.351 0.385 0.392 0.378 0.368 0.456 1.408 1.407 1.575 NOT TAKEN 1.534 0.372 SPILLED 0.368 0.336 0.398 0.395 0.381 SPILLED 0.425 0.408 0.365 0.336 0.410 SPILLED 0.393 0.346 0.461 0.370 0.368 0.387 0.385 0.379 SPILLED 1.409 1.350 1.631 NOT TAKEN 1.536 0.330 0.387 0.355 0.305 0.366 0.373 0.358 SPILLED 0.390 SPILLED SPILLED 0.352 0.390 0.365 0.402 0.381 SPILLED 0.358 0.334 0.357 0.366 0.372 SPILLED 1.317 1.288 1.621 NOT TAKEN 1.555 0.338 0.349 0.317 0.330 0.364 0.378 0.367 0.349 0.374 SPILLED SPILLED 0.310 0.392 0.347 0.370 0.358 0.426 0.331 0.334 0.318 0.360 0.340 SPILLED 1.217 1.199 1.562 NOT TAKEN 1.432 0.374 0.377 0.349 0.322 0.374 0.347 0.360 0.348 0.391 SPILLED 0.379 0.303 0.382 0.327 SPILLED 0.328 SPILLED 0.375 0.364 0.327 0.368 0.345 SPILLED SPILLED 1.322 1.645 NOT TAKEN 1.487 1447 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 17 ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 WEEK 9 WEEK 10 GROUP: C93116 C93117 C93118 C93119 C93120 C93121 C93122 C93123 C93124 C93125 C93126 C93127 C93128 C93129 C93130 C93131 C93132 C93133 C93134 C93135 C93136 C93137 C93138 C93139 C93140 C93141 C93142 C93143 C93144 C93145 C93146 FEMALE 5 - 2 0 PPM 2.068 2.117 1.953 2.114 2.296 SPILLED 2.121 2.027 SPILLED 2.035 1.975 2.101 1.803 2.344 2.145 2.190 2.122 2.194 2.016 2.399 2.035 2.331 1.927 2.492 2.314 2.238 2.307 2.095 SPILLED 2.131 2.060 1.815 1.932 1.981 1.808 2.016 1.869 2.040 2.122 SPILLED 2.101 1.795 1.801 1.915 2.159 2.002 1.862 1.816 1.826 1.648 1.980 1.694 2.348 1.783 2.269 2.139 SPILLED 1.891 2.006 2.145 1.888 1.671 1.691 SPILLED 1.845 1.745 1.788 1.751 SPILLED 1.701 2.110 1.954 SPILLED 1.714 1.616 2.045 1.743 1.737 1.660 1.732 1.550 1.878 1.525 2.071 1.619 SPILLED 2.043 2.104 1.811 SPILLED 1.984 SPILLED 1.546 1.659 1.656 1.727 1.695 1.621 1.510 1.553 NOT TAKEN SPILLED 1.813 1.521 1.764 1.642 1.858 1.819 1.551 1.465 1.587 1.524 1.721 1.531 1.689 1.547 1.745 1.729 SPILLED NOT TAKEN SPILLED NOT TAKEN 1.842 1.526 SPILLED SPILLED 1.678 1.621 SPILLED SPILLED 1.683 NOT TAKEN SPILLED 1.783 1.516 1.530 1.542 1.756 1.707 1.531 1.461 1.541 1.506 1.761 1.493 1.864 1.562 1.827 1.836 2.084 NOT TAKEN 1.766 NOT TAKEN SPILLED SPILLED 1.516 SPILLED 1.455 1.488 1.568 SPILLED 1.317 NOT TAKEN SPILLED 1.684 1.391 1.302 1.438 1.641 1.458 1.468 1.306 1.339 1.473 1.501 1.375 1.697 1.413 SPILLED 1.606 1.522 NOT TAKEN SPILLED NOT TAKEN 1.756 SPILLED SPILLED 1.471 1.587 1.453 1.481 1.509 1.325 NOT TAKEN 1.512 SPILLED 1.805 1.513 1.483 1.547 1.592 1.341 1.328 SPILLED 1.340 1.519 1.340 1.669 1.431 1.592 1.554 SPILLED NOT TAKEN 1.666 NOT TAKEN 1.635 1.673 SPILLED 1.458 1.429 1.303 1.480 1.353 SPILLED NOT TAKEN 1.300 1.656 1.330 1.511 1.366 1.494 1.522 1.327 1.229 1.392 1.349 1.462 1.411 1.493 1.363 1.485 1.609 1.744 NOT TAKEN SPILLED NOT TAKEN SPILLED 1.392 1.387 SPILLED 1.479 1.404 1.306 1.337 1.371 NOT TAKEN 1.411 1.398 1.290 1.289 1.405 1.490 1.415 1.300 1.322 1.362 1.213 1.448 1.329 1.419 1.370 1.520 1.369 SPILLED NOT TAKEN 1.421 NOT TAKEN 1.597 SPILLED 1.414 1.367 1.450 1.439 1.485 1.366 1.268 NOT TAKEN 1.613 1.636 1.290 1.369 1.326 1.398 1.407 1.209 1.401 1.387 1.301 1.349 1.457 1.535 1.313 1.598 1.468 1.459 NOT TAKEN SPILLED NOT TAKEN 1.441 1.463 1448 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 18 ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 WEEK 9 WEEK 10 GROUP: C93147 C93148 C93149 C93150 C93151 C93152 C93153 C93154 C93155 C93156 C93157 C93158 C93159 C93160 C93161 C93162 C93163 C93164 C93165 C93166 C93167 C93168 C93169 C93170 C93171 C93172 C93173 C93174 C93175 C93176 C93177 FEMALE 5 - 20 PPM 2.107 2.215 2.124 2.491 2.087 2.178 SPILLED 2.258 2.347 2.169 2.172 2.201 2.114 2.395 2.118 2.235 2.020 2.303 2.187 2.025 1.893 2.449 2.087 2.075 SPILLED 2.035 2.094 2.099 2.127 2.207 2.043 1.915 1.875 SPILLED 2.299 1.779 2.107 SPILLED 2.024 2.276 1.944 1.884 1.950 1.825 2.125 SPILLED 2.056 1.926 1.957 1.992 1.783 2.004 2.461 1.940 1.945 SPILLED 1.749 2.049 SPILLED 2.049 1.949 1.852 1.721 1.579 2.004 1.970 1.621 SPILLED 2.020 1.748 2.088 1.770 2.006 1.692 1.716 1.901 SPILLED 2.004 SPILLED 1.609 1.815 1.666 1.704 1.993 1.773 1.855 SPILLED 1.639 1.951 SPILLED 1.787 1.855 1.742 1.681 1.480 1.867 1.881 1.416 1.583 SPILLED 1.640 1.494 1.549 1.622 1.543 NOT TAKEN 1.740 1.693 1.648 1.564 SPILLED 1.636 1.644 1.483 1.887 1.787 1.684 SPILLED SPILLED 1.419 1.777 1.533 1.684 1.607 1.651 1.565 2.032 1.823 1.606 1.513 SPILLED 1.669 1.697 1.637 1.732 1.706 NOT TAKEN 1.807 1.664 1.665 1.548 SPILLED 1.734 1.505 1.596 1.850 1.654 1.825 SPILLED 1.502 1.609 1.824 1.642 1.668 1.564 1.578 1.526 SPILLED 1.557 1.474 1.596 SPILLED 1.507 1.651 1.479 1.672 1.413 NOT TAKEN 1.551 SPILLED 1.637 1.527 1.575 1.569 1.487 1.370 1.752 1.456 1.505 SPILLED 1.426 1.454 1.555 1.403 1.454 1.435 1.517 1.631 SPILLED 1.516 1.452 SPILLED SPILLED 1.480 1.803 1.515 1.619 1.335 NOT TAKEN 1.495 SPILLED 1.458 1.419 1.503 1.462 SPILLED 1.319 1.605 1.467 1.442 SPILLED 1.489 SPILLED SPILLED 1.530 1.468 1.495 1.376 1.450 1.828 1.579 1.423 1.415 SPILLED 1.443 1.913 1.422 1.467 1.466 NOT TAKEN 1.479 1.445 SPILLED 1.286 SPILLED 1.358 1.351 1.376 1.480 1.396 SPILLED SPILLED 1.283 SPILLED SPILLED 1.314 1.362 1.373 1.365 1.405 1.849 1.611 1.520 1.385 SPILLED 1.398 1.591 1.332 1.503 1.375 NOT TAKEN 1.402 SPILLED 1.522 1.293 1.438 1.385 1.216 1.162 1.390 1.417 1.524 SPILLED SPILLED 1.504 SPILLED 1.390 1.337 1.650 1.295 1.298 SPILLED 1.545 1.359 1.306 SPILLED 1.394 SPILLED 1.319 1.487 1.393 NOT TAKEN 1.351 1.352 SPILLED 1.337 1.328 1.324 1.255 1.269 SPILLED 1.285 SPILLED SPILLED 1.431 SPILLED SPILLED 1.349 1.302 1.528 1449 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104 -WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 19 ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 WEEK 9 WEEK 10 GROUP: C93178 C93179 C93180 GROUP: C93181 C93182 C93183 C93184 C93185 C93186 C93187 C93188 C93189 C93190 C93191 C93192 C93193 C93194 C93195 C93196 C93197 C93198 C93199 C93200 C93201 C93202 C93203 C93204 C93205 FEMALE 5 - 20 PPM SPILLED 2.080 SPILLED 1.935 1.831 2.033 FEMALE 6 - 20 PPM 2.207 2.118 2.137 2.243 SPILLED 2.049 2.136 2.299 SPILLED 2.368 2.107 1.933 2.182 1.981 2.399 2.230 1.905 2.177 2.073 2.390 SPILLED 1.950 2.191 2.355 2.039 1.953 1.952 1.815 1.887 2.434 1.944 1.985 1.861 SPILLED 1.865 2.045 1.682 2.104 1.801 SPILLED 2.349 1.675 1.930 1.711 2.006 2.028 1.993 1.900 2.012 SPILLED 1.859 1.659 SPILLED 1.846 1.900 1.792 1.957 SPILLED 1.840 1.683 1.822 SPILLED 1.773 1.552 1.553 1.896 1.864 1.796 SPILLED 1.595 1.817 1.690 1.949 1.929 1.710 1.602 1.967 1.945 1.763 1.573 1.555 1.655 1.690 1.557 1.814 SPILLED 1.603 1.835 1.380 SPILLED 1.553 1.621 1.751 1.691 1.759 1.533 SPILLED 1.542 1.774 1.508 1.993 1.655 1.629 1.733 1.732 1.488 1.552 1.602 1.605 1.802 1.682 1.549 1.739 SPILLED 1.557 1.496 1.541 SPILLED 1.634 1.514 1.579 1.645 1.634 1.509 SPILLED 1.436 1.660 1.506 1.992 1.618 1.571 1.505 1.751 1.486 1.474 1.464 1.439 1.665 1.393 1.524 1.544 1.685 1.635 1.501 1.397 1.566 1.478 1.423 1.464 1.583 1.531 1.390 SPILLED 1.391 1.481 1.453 1.573 1.384 SPILLED 1.649 1.609 1.391 1.464 1.410 1.385 1.544 1.478 1.362 1.583 1.526 1.402 1.518 1.412 1.602 1.460 1.448 1.517 1.446 1.573 1.343 1.606 1.418 1.403 1.298 SPILLED 1.315 1.263 1.439 1.521 1.457 1.320 1.376 1.408 1.546 1.304 1.296 1.504 1.693 1.396 1.341 1.366 1.544 1.350 1.429 1.323 1.350 1.374 SPILLED 1.492 1.322 1.247 1.347 SPILLED SPILLED SPILLED SPILLED 1.463 1.332 1.373 1.377 SPILLED 1.507 1.426 1.283 1.410 1.532 1.449 1.384 1.314 1.526 1.415 1.251 1.247 1.237 1.473 1.663 1.543 1.377 1.366 1.292 2.433 1.429 SPILLED 1.415 1.455 1.338 1.323 SPILLED 1.356 1.494 1.293 1.399 1.404 1.634 1.569 1.306 1.304 1.497 1.437 1.381 1.355 1.472 1.705 1.328 1.435 1.381 1.395 1.297 SPILLED 1.333 SPILLED 1.303 1.562 1.365 1450 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 20 ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 WEEK 9 WEEK 10 GROUP: C93206 C93207 C93208 C93209 C93210 C93211 C93212 C93213 C93214 C93215 C93216 C93217 C93218 C93219 C93220 FEMALE 6 - 20 PPM 2.364 2.324 1.992 1.931 2.276 1.862 2.049 2.016 2.111 2.639 2.076 SPILLED 2.435 2.040 SPILLED 1.910 2.120 1.756 1.798 1.815 SPILLED 2.052 1.811 1.862 1.931 1.745 SPILLED 1.949 1.892 SPILLED 1.775 1.855 1.733 SPILLED 1.689 1.787 1.830 1.748 1.651 SPILLED 1.707 SPILLED 2.079 1.811 SPILLED 1.554 1.665 1.429 SPILLED 2.035 1.653 1.796 1.575 SPILLED 1.618 1.565 SPILLED 1.667 1.613 SPILLED 1.765 1.653 1.487 SPILLED 1.629 1.596 1.722 1.520 SPILLED 1.603 1.584 SPILLED 1.679 1.579 SPILLED SPILLED 1.416 1.211 SPILLED SPILLED 1.399 1.479 1.414 SPILLED 1.469 1.407 1.628 SPILLED SPILLED SPILLED 1.483 1.498 1.429 1.312 1.401 1.416 1.572 1.343 1.429 1.380 1.363 1.498 SPILLED SPILLED SPILLED SPILLED 1.550 1.279 SPILLED 1.396 1.422 1.456 1.281 1.377 1.398 1.310 1.326 SPILLED 1.346 SPILLED SPILLED 1.407 1.213 1.423 1.372 1.561 1.481 1.305 1.432 1.310 1.376 1.294 SPILLED 1.245 SPILLED 1.571 1.380 1.262 SPILLED 1.295 1.499 1.520 1.311 1.417 1.312 1.277 1.345 SPILLED SPILLED SPILLED 1451 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 21 ANIMAL NUMBER WEEK 11 WEEK 12 WEEK 13 WEEK 14 WEEK 15 WEEK 16 WEEK 20 WEEK 24 WEEK 28 WEEK 32 GROUP: MALE 2 - 0 . 5 PPM C92571 C92572 C92573 C92574 C92575 C92576 C92577 C92578 C92579 C92580 C92581 C92582 C92583 C92584 C92585 C92586 C92587 C92588 C92589 C92590 C92591 C92592 C92593 C92594 C92595 C92596 C92597 C92598 C92599 C92600 C92601 0.031 0.028 0.025 NOT TAKEN NOT TAKEN 0.025 0.026 0.027 0.026 0.026 0.024 0.025 0.027 0.029 CNTMD 0.026 0.025 SPILLED 0.027 0.026 0.026 0.024 0.025 0.028 TECH SPILL 0.028 SPILLED 0.026 0.027 0.027 0.024 0.030 0.028 0.025 NOT TAKEN NOT TAKEN 0.025 0.026 0.026 0.025 0.027 0.024 0.025 0.026 0.030 CNTMD 0.026 0.025 0.026 0.025 0.027 0.025 0.024 0.024 0.028 0.025 0.027 0.028 0.026 0.027 0.025 0.025 0 .029 0 .027 0 .024 NOT TAKEN NOT TAKEN 0 .025 0 .025 0 .026 0 .025 0 .026 0 .024 0 .024 0 .024 0 .029 0 .027 0 .026 0 .024 0 .026 0 .024 0 .026 0 .024 0 .023 0 .023 0 .026 0 .024 0 .027 0 .028 0 .025 0 .026 0 .025 0 .024 0 .027 0 .027 0 .023 NOT TAKEN NOT TAKEN 0 .024 0 .024 0 .026 0 .023 0 .025 0 .023 0 .024 0 .022 0 .027 0 .024 0 .026 0 .024 0 .025 0 .024 0 .025 0 .024 0 .024 NOT TAKEN 0 .026 0 .024 0 .026 0 .027 0 .022 0 .026 NOT TAKEN 0 .023 0.027 0.026 0.023 NOT TAKEN NOT TAKEN 0.024 0.023 0.024 0.023 0.025 0.022 0.024 0.027 0.029 CNTMD 0.023 0.024 0.027 0.024 0.025 0.023 0.023 NOT TAKEN 0.027 0.026 0.025 0.028 0.024 0.025 NOT TAKEN 0.023 0 .025 0 .026 0 .023 NOT TAKEN NOT TAKEN 0 .024 0 .024 0 .025 0 .022 0 .025 0 .022 0 .024 0 .025 0 .027 0 .024 0 .025 0 .024 0 .026 0 .023 0 .024 0 .024 0 .024 NOT TAKEN 0 .026 0 .024 0 .025 0 .029 0 .024 0 .025 NOT TAKEN 0 .024 0 .023 0 .025 0 .021 NOT TAKEN NOT TAKEN 0 .025 0 .023 0 .024 0 .023 0 .025 0 .023 0 .024 0 .023 0 .026 0 .025 0 .025 0 .024 SPILLED 0 .023 0 .022 0 .024 0 .023 NOT TAKEN 0 .025 0 .022 0 .026 0 .027 0 .025 0 .024 NOT TAKEN 0 .023 0 .021 0 .022 0 .020 NOT TAKEN NOT TAKEN 0 .021 0 .020 0 .022 0 .021 0 .021 0 .020 0 .022 0 .022 0 .024 0 .023 0 .021 0 .022 0 .023 0 .022 0 .022 0 .022 0 .022 NOT TAKEN 0 .023 0 .021 0 .024 0 .025 0 .022 0 .022 NOT TAKEN 0 .022 0 .019 0 .021 0 .019 NOT TAKEN NOT TAKEN 0 .021 0 .019 0 .020 0 .019 0 .020 0 .019 0 .023 0 .021 0 .025 0 .021 0 .020 0 .020 0 .021 0 .020 SPILLED 0 .021 0 .020 NOT TAKEN 0 .022 0 .021 0 .022 0 .025 0 .022 0 .022 NOT TAKEN 0 .021 0 .020 0 .020 0 .018 NOT TAKEN NOT TAKEN 0 .019 0 .018 0 .019 0 .018 0 .019 0 .019 0 .021 0 .020 0 .023 0 .019 0 .020 0 .018 0 .020 0 .019 0 .018 0 .019 0 .018 NOT TAKEN 0 .020 0 .019 0 .020 0 .022 0 .020 0 .022 NOT TAKEN 0 .020 1452 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 22 ANIMAL NUMBER WEEK 11 WEEK 12 WEEK 13 WEEK 14 WEEK 15 WEEK 16 WEEK 20 WEEK 24 WEEK 28 WEEK 32 GROUP: MALE 2 - 0.5 PPM C92602 C92603 C92604 C92605 C92606 C92607 C92608 C92609 C92610 C92611 C92612 C92613 C92614 C92615 C92616 C92617 C92618 C92619 C92620 C92621 C92622 C92623 C92624 C92625 C92626 C92627 C92628 C92629 C92630 0.028 0.027 0.026 0.026 0.029 0.028 SPILLED 0.026 NOT TAKEN 0.025 0.026 NOT TAKEN SPILLED 0.027 0.031 0.026 NOT TAKEN 0.028 0.026 SPILLED 0.026 0.026 0.027 0.027 0.026 SPILLED 0.028 SPILLED 0.032 0.029 0.027 0.026 0.026 0.028 0.027 0.028 0.025 NOT TAKEN 0.025 0.024 NOT TAKEN SPILLED 0.027 0.029 0.026 NOT TAKEN 0.027 0.027 0.028 0.025 0.026 0.025 0.026 0.025 SPILLED SPILLED 0.028 0.031 0.028 0.024 0.025 0.024 0.026 0.026 0.025 0.025 NOT TAKEN 0.024 0.023 NOT TAKEN SPILLED 0.025 0.029 0.024 NOT TAKEN 0.026 0.030 0.027 0.023 0.024 0.025 0.024 0.024 SPILLED SPILLED 0.027 0.030 0.028 0.025 0.024 0.024 0.025 0.027 0.024 0.025 NOT TAKEN 0.024 0.021 NOT TAKEN SPILLED 0.025 NOT TAKEN 0.024 NOT TAKEN 0.026 0.028 NOT TAKEN 0.024 0.023 0.023 0.024 0.023 NOT TAKEN 0.028 0.024 0.029 0.027 0.023 0.024 0.024 0.024 0.025 0.015 0.025 NOT TAKEN 0.024 0.024 NOT TAKEN SPILLED 0.025 NOT TAKEN 0.024 NOT TAKEN 0.027 0.026 NOT TAKEN 0.023 0.024 0.025 0.025 0.023 NOT TAKEN SPILLED 0.026 0.030 0.026 0.025 0.024 0.024 0.026 0.025 0.021 0.025 NOT TAKEN 0.023 0.024 NOT TAKEN SPILLED 0.024 NOT TAKEN 0.023 NOT TAKEN 0.025 0.026 NOT TAKEN 0.024 0.025 0.025 0.024 0.019 NOT TAKEN 0.026 0.026 0.030 0.026 NOT TAKEN 0.023 0.021 0.025 0.024 0.025 0.025 NOT TAKEN 0.022 0.022 NOT TAKEN SPILLED 0.022 NOT TAKEN 0.022 NOT TAKEN 0.024 0.024 NOT TAKEN 0.022 0.023 0.023 0.021 0.023 NOT TAKEN 0.026 0.026 SPILLED 0.023 NOT TAKEN 0.021 0.021 0.022 0.023 0.022 0.023 NOT TAKEN 0.021 0.015 NOT TAKEN SPILLED 0.021 NOT TAKEN 0.021 NOT TAKEN 0.021 0.022 NOT TAKEN 0.022 0.021 0.021 0.020 0.022 NOT TAKEN 0.023 0.021 0.025 0.024 NOT TAKEN 0.020 0.019 0.019 0.019 0.020 0.023 NOT TAKEN 0.019 0.019 NOT TAKEN SPILLED 0.019 NOT TAKEN 0.019 NOT TAKEN 0.021 0.021 NOT TAKEN 0.019 0.020 0.020 0.019 0.018 NOT TAKEN 0.023 0.021 SPILLED 0.022 NOT TAKEN 0.019 0.017 0.019 0.020 0.019 0.021 NOT TAKEN 0.020 0.019 NOT TAKEN SPILLED 0.018 NOT TAKEN 0.018 NOT TAKEN 0.020 0.021 NOT TAKEN 0.018 0.018 0.020 0.017 SPILLED NOT TAKEN SPILLED 0.019 SPILLED 1453 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 23 ANIMAL NUMBER WEEK 11 WEEK 12 WEEK 13 WEEK 14 WEEK 15 WEEK 16 WEEK 20 WEEK 24 WEEK 28 WEEK 32 GROUP: C92631 C92632 C92633 C92634 C92635 C92636 C92637 C92638 C92639 C92640 C92641 C92642 C92643 C92644 C92645 C92646 C92647 C92648 C92649 C92650 C92651 C92652 C92653 C92654 C92655 C92656 C92657 C92658 C92659 C92660 C92661 MALE 3 - 2 PPM 0.110 0.100 0.106 0.119 SPILLED SPILLED 0.107 0.112 0.106 0.110 0.079 0.104 0.112 0.114 0.117 NOT TAKEN 0.103 0.105 0.119 NOT TAKEN 0.109 NOT TAKEN 0.104 0.110 SPILLED 0.101 SPILLED 0.106 0.108 0.111 0.098 0.108 0.096 0.104 0.115 0.115 0.112 0.108 SPILLED 0.104 0.112 0.099 0.101 0.107 0.111 0.119 NOT TAKEN 0.104 0.108 0.121 NOT TAKEN 0.110 NOT TAKEN 0.103 0.112 SPILLED 0.099 SPILLED 0.107 0.110 0.104 0.097 0.104 0.090 0.099 0.114 0.107 0.113 0.104 0.100 0.102 0.110 0.106 0.102 0.106 0.106 0.118 NOT TAKEN 0.101 0.103 0.121 NOT TAKEN 0.103 NOT TAKEN 0.101 0.105 SPILLED 0.098 0.105 0.103 0.105 0.103 0.098 0.101 0.089 0.098 0.119 0.105 0.099 0.105 0.104 0.100 NOT TAKEN 0.106 0.094 0.106 0.096 NOT TAKEN NOT TAKEN 0.097 0.107 0.117 NOT TAKEN 0.092 NOT TAKEN 0.101 0.107 0.121 0.091 0.101 0.099 0.098 0.106 0.088 0.100 0.106 0.095 0.119 0.100 SPILLED 0.105 0.095 0.098 NOT TAKEN 0.103 0.095 0.100 0.111 NOT TAKEN NOT TAKEN 0.101 0.107 0.117 NOT TAKEN 0.102 NOT TAKEN 0.095 0.104 SPILLED 0.093 0.102 0.099 0.108 0.102 0.104 0.105 0.090 0.100 0.105 0.100 0.109 0.103 0.100 0.101 NOT TAKEN 0.106 0.094 SPILLED 0.107 NOT TAKEN NOT TAKEN 0.100 0.105 0.113 NOT TAKEN 0.102 NOT TAKEN 0.091 0.099 SPILLED 0.091 0.097 0.093 0.101 0.103 0.101 0.097 0.086 0.099 0.111 0.095 0.100 0.102 0.091 0.093 NOT TAKEN 0.098 0.086 0.096 0.100 NOT TAKEN NOT TAKEN 0.094 0.103 0.113 NOT TAKEN 0.092 NOT TAKEN 0.093 0.093 0.107 0.085 0.099 0.093 0.096 0.115 0.091 0.091 0.085 0.089 0.100 0.093 0.089 0.095 0.091 0.094 NOT TAKEN 0.091 0.081 0.097 0.094 NOT TAKEN NOT TAKEN 0.089 0.094 0.097 NOT TAKEN 0.093 NOT TAKEN 0.082 0.094 0.103 0.079 0.089 0.081 0.084 0.108 0.087 0.089 0.074 0.084 0.087 0.083 0.084 0.088 0.078 0.083 NOT TAKEN 0.071 0.079 0.089 0.088 NOT TAKEN NOT TAKEN 0.082 0.088 0.091 NOT TAKEN 0.083 NOT TAKEN 0.078 0.085 0.099 0.074 0.080 0.079 0.083 0.095 0.081 0.081 0.070 0.076 0.082 0.080 0.082 0.085 0.075 0.075 NOT TAKEN 0.075 0.073 0.084 0.091 NOT TAKEN NOT TAKEN 0.080 0.082 0.090 NOT TAKEN 0.080 NOT TAKEN 0.075 0.082 0.091 0.071 0.079 0.076 0.079 SPILLED 0.073 1454 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 24 ANIMAL NUMBER WEEK 11 WEEK 12 WEEK 13 WEEK 14 WEEK 15 WEEK 16 WEEK 20 WEEK 24 WEEK 28 WEEK 32 GROUP: MALE 3 - 2 PPM C92662 C92663 C92664 C92665 C92666 C92667 C92668 C92669 C92670 C92671 C92672 C92673 C92674 C92675 C92676 C92677 C92678 C92679 C92680 C92681 C92682 C92683 C92684 C92685 C92686 C92687 C92688 C92689 C92690 0.106 0.112 0.110 0.103 0.116 0.110 NOT TAKEN SPILLED 0.111 0.115 0.107 0.102 SPILLED 0.115 0.109 0.102 NOT TAKEN 0.110 0.107 0.107 0.106 0.108 0.117 0.105 0.100 0.108 SPILLED 0.104 0.099 0.104 0.112 0.107 0.099 0.118 0.106 NOT TAKEN 0.123 0.110 0.109 0.105 0.103 0.111 0.107 0.101 0.098 NOT TAKEN 0.104 0.111 0.105 0.103 0.105 0.064 0.097 0.099 0.104 0.121 0.093 0.097 0.108 0.113 0.102 0.103 0.113 0.106 NOT TAKEN 0.119 0.110 0.108 0.097 0.103 0.110 0.107 0.100 0.099 NOT TAKEN 0.105 0.104 0.097 0.103 0.103 0.105 0.098 0.099 0.103 0.119 0.097 0.097 NOT TAKEN 0.112 0.103 0.098 0.110 0.102 NOT TAKEN SPILLED 0.105 0.103 0.101 0.099 0.112 0.105 NOT TAKEN 0.101 NOT TAKEN 0.101 0.101 0.098 0.090 0.102 NOT TAKEN 0.101 0.101 0.099 SPILLED 0.099 0.095 NOT TAKEN 0.107 0.102 0.097 0.107 0.092 NOT TAKEN 0.122 0.105 0.104 0.100 0.096 0.112 0.103 NOT TAKEN 0.102 NOT TAKEN 0.103 0.100 0.097 0.101 0.100 NOT TAKEN 0.099 0.095 0.099 SPILLED 0.102 0.093 NOT TAKEN 0.106 0.103 0.096 0.113 0.095 NOT TAKEN 0.109 0.102 0.104 0.097 0.096 0.103 0.106 NOT TAKEN 0.104 NOT TAKEN 0.102 0.093 0.102 0.096 0.094 NOT TAKEN 0.095 0.097 0.091 0.113 0.099 0.088 NOT TAKEN 0.100 0.102 0.088 0.109 0.093 NOT TAKEN 0.099 0.099 0.097 0.086 0.091 0.097 0.100 NOT TAKEN 0.101 NOT TAKEN 0.096 0.090 0.091 0.096 0.097 NOT TAKEN 0.091 0.095 0.088 SPILLED 0.092 0.089 NOT TAKEN 0.097 0.090 0.083 0.115 0.082 NOT TAKEN 0.093 0.090 0.090 0.086 0.081 0.094 0.096 NOT TAKEN 0.094 NOT TAKEN 0.089 0.083 0.090 0.086 0.092 NOT TAKEN 0.083 0.089 0.085 SPILLED 0.090 0.077 NOT TAKEN 0.085 0.080 0.077 0.104 0.082 NOT TAKEN 0.085 0.088 0.085 0.076 0.074 0.079 0.089 NOT TAKEN 0.090 NOT TAKEN 0.086 0.079 SPILLED 0.079 0.083 NOT TAKEN 0.074 0.083 0.080 SPILLED 0.080 0.075 NOT TAKEN 0.080 0.082 0.075 SPILLED 0.078 NOT TAKEN 0.082 0.082 0.085 0.075 0.076 0.076 0.090 NOT TAKEN 0.085 NOT TAKEN 0.082 0.074 0.077 0.079 0.080 NOT TAKEN 0.076 0.076 0.074 0.097 0.076 0.074 1455 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 25 ANIMAL NUMBER WEEK 11 WEEK 12 WEEK 13 WEEK 14 WEEK 15 WEEK 16 WEEK 20 WEEK 24 WEEK 28 WEEK 32 GROUP: MALE 4 - 5 PPM C92691 C92692 C92693 C92694 C92695 C92696 C92697 C92698 C92699 C92700 C92701 C92702 C92703 C92704 C92705 C92706 C92707 C92708 C92709 C92710 C92711 C92712 C92713 C92714 C92715 C92716 C92717 C92718 C92719 C92720 C92721 0.265 0.249 0.271 0.240 0.261 0.279 0.304 0.260 0.273 0.270 0.252 0.249 0.294 0.261 0.283 0.241 0.250 0.264 0.267 0.275 0.243 0.267 0.256 0.278 NOT TAKEN 0.242 0.269 NOT TAKEN 0.278 SPILLED 0.260 0.254 0.246 0.256 0.229 0.259 SPILLED 0.295 0.254 0.270 0.269 0.247 0.251 0.298 0.251 0.275 0.246 0.235 0.237 0.262 0.275 0.240 0.258 0.256 0.265 NOT TAKEN 0.247 0.275 NOT TAKEN 0.275 SPILLED 0.259 0.249 0.246 0.245 0.209 0.248 0.266 0.288 0.253 0.258 0.270 0.244 0.239 0.283 0.240 0.260 0.235 0.234 0.260 0.261 0.255 0.239 0.263 0.242 0.263 NOT TAKEN 0.240 0.257 NOT TAKEN 0.272 SPILLED 0.260 0.244 0.245 0.249 0.228 0.246 0.248 SPILLED 0.247 0.256 0.261 0.237 0.238 0.279 0.245 0.265 0.230 0.233 0.250 0.249 00..220511 0.278 NOT TAKEN NOT TAKEN NOT TAKEN 0.204 0.258 NOT TAKEN NOT TAKEN SPILLED 0.246 0.257 0.234 0.252 0.234 0.244 0.249 0.260 0.243 0.248 0.257 0.227 0.236 0.264 0.235 0.266 0.231 0.236 0.256 0.260 0.247 0.239 0.273 NOT TAKEN NOT TAKEN NOT TAKEN SPILLED 0.256 NOT TAKEN NOT TAKEN SPILLED 0.251 0.251 0.229 0.242 0.239 0.247 0.245 0.272 0.226 0.247 0.263 0.226 0.232 0.273 0.236 0.251 0.216 0.232 0.249 0.247 0.238 0.241 0.257 NOT TAKEN NOT TAKEN NOT TAKEN 0.231 0.264 NOT TAKEN NOT TAKEN 0.287 0.262 0.244 00..222019 0.225 0.227 0.243 0.269 0.226 0.179 0.262 0.227 0.219 00..222522 0.248 0.207 0.225 0.230 0.231 0.226 0.219 SPILLED NOT TAKEN NOT TAKEN NOT T0A.2K2E0N 0.245 NOT TAKEN NOT TAKEN SPILLED 0.230 0.222 00..211915 0.195 0.214 0.236 0.249 0.198 0.227 0.228 0.200 0.205 00..220213 0.228 0.193 0.206 0.207 0.217 0.208 0.204 0.233 NOT TAKEN NOT TAKEN NOT TAKEN 0.203 0.215 NOT TAKEN NOT TAKEN 0.254 0.227 0.206 0.193 0.194 0.192 0.213 SPILLED 0.223 0.183 0.203 0.248 00..210917 0.206 0.193 0.218 0.194 0.200 0.201 0.207 0.209 0.193 0.219 NOT TAKEN NOT TAKEN NOT TAKEN 0.191 0.207 NOT TAKEN NOT TAKEN 00..222329 0.199 0.180 0.195 0.187 0.199 0.186 0.224 0.183 0.224 SPILLED 0.187 0.206 0.198 0.179 0.197 0.190 0.201 0.199 0.193 0.194 0.189 0.209 NOT TAKEN NOT TAKEN NOT TAKEN 0.191 0.204 NOT TAKEN NOT TAKEN 0.224 0.204 1456 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 26 ANIMAL NUMBER WEEK 11 WEEK 12 WEEK 13 WEEK 14 WEEK 15 WEEK 16 WEEK 20 WEEK 24 WEEK 28 WEEK 32 GROUP: MALE 4 - 5 PPM C92722 C92723 C92724 C92725 C92726 C92727 C92728 C92729 C92730 C92731 C92732 C92733 C92734 C92735 C92736 C92737 C92738 C92739 C92740 C92741 C92742 C92743 C92744 C92745 C92746 C92747 C92748 C92749 C92750 0.297 SPILLED SPILLED 0.268 0.264 0.264 0.271 0.268 SPILLED NOT TAKEN 0.282 0.283 NOT TAKEN 0.254 0.256 0.266 0.273 SPILLED 0.265 0.256 0.257 SPILLED NOT TAKEN 0.265 SPILLED 0.266 SPILLED SPILLED SPILLED 0.291 SPILLED SPILLED 0.256 0.253 0.281 0.266 0.269 0.262 NOT TAKEN 0.293 0.273 NOT TAKEN 0.253 0.258 0.257 0.265 0.297 0.259 0.250 0.248 0.254 NOT TAKEN 0.261 SPILLED 0.271 SPILLED 0.275 0.266 0.274 SPILLED 0.310 0.252 0.248 0.275 0.254 0.272 0.254 NOT TAKEN 0.281 0.275 NOT TAKEN 0.249 0.253 0.247 0.251 0.281 0.252 0.251 0.242 0.255 NOT TAKEN 0.252 0.225 0.258 SPILLED 0.241 0.257 NOT TAKEN SPILLED SPILLED 0.252 0.217 0.243 NOT TAKEN 0.254 0.257 NOT TAKEN 0.270 0.287 NOT TAKEN 0.249 0.246 0.252 0.252 0.269 0.248 0.225 0.230 0.253 NOT TAKEN 0.252 0.218 0.261 SPILLED 0.263 0.254 NOT TAKEN SPILLED SPILLED 0.242 0.272 0.248 NOT TAKEN 0.235 0.257 NOT TAKEN 0.267 0.286 NOT TAKEN 0.244 0.245 0.251 0.255 0.267 0.240 0.249 0.227 0.245 NOT TAKEN 0.244 0.229 0.255 SPILLED 0.263 0.252 NOT TAKEN 0.256 0.294 0.243 0.249 0.261 NOT TAKEN 0.237 0.252 NOT TAKEN 0.273 0.260 NOT TAKEN 0.254 0.231 0.241 0.229 0.273 0.236 0.236 0.220 0.239 NOT TAKEN 0.249 0.222 0.243 SPILLED 0.252 0.237 NOT TAKEN SPILLED 0.258 0.228 0.222 0.244 NOT TAKEN 0.229 0.226 NOT TAKEN 0.247 0.283 NOT TAKEN 0.223 0.238 0.235 0.227 0.270 0.229 0.253 0.199 0.235 NOT TAKEN 0.227 0.220 0.236 0.277 0.248 0.239 NOT TAKEN 0.242 0.245 0.204 0.235 0.224 NOT TAKEN 0.213 0.223 NOT TAKEN 0.218 0.255 NOT TAKEN 0.206 0.223 0.210 0.234 0.260 0.205 0.222 0.189 0.200 NOT TAKEN 0.213 0.205 0.223 0.258 0.222 0.215 NOT TAKEN SPILLED SPILLED 0.194 0.215 0.197 NOT TAKEN 0.197 0.212 NOT TAKEN 0.213 0.248 NOT TAKEN 0.201 0.204 0.204 0.208 0.228 0.193 SPILLED 0.180 0.191 NOT TAKEN 0.189 0.193 0.216 0.231 0.225 0.205 NOT TAKEN SPILLED 0.209 0.190 0.203 0.203 NOT TAKEN 0.190 0.194 NOT TAKEN 0.203 0.228 NOT TAKEN 0.202 0.190 0.198 0.205 0.222 0.197 SPILLED 0.177 0.192 NOT TAKEN 0.201 0.186 0.200 0.220 0.206 0.194 1457 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 27 ANIMAL NUMBER WEEK 11 WEEK 12 WEEK 13 WEEK 14 WEEK 15 WEEK 16 WEEK 20 WEEK 24 WEEK 28 WEEK 32 GROUP: MALE 5 - 2 0 PPM C92751 C92752 C92753 C92754 C92755 C92756 C92757 C92758 C92759 C92760 C92761 C92762 C92763 C92764 C92765 C92766 C92767 C92768 C92769 C92770 C92771 C92772 C92773 C92774 C92775 C92776 C92777 C92778 C92779 C92780 C92781 0.991 NOT TAKEN 0.884 1.033 1.068 1.194 1.127 0.978 NOT TAKEN 1.073 1.077 1.170 1.006 1.206 1.064 1.063 1.154 0.999 1.080 1.004 0.976 0.998 1.019 1.043 1.115 1.017 1.132 1.019 NOT TAKEN SPILLED 1.020 0.902 NOT TAKEN SPILLED 1.107 1.051 1.276 1.099 0.962 NOT TAKEN 1.144 1.045 1.139 1.043 1.260 1.006 1.078 1.131 0.977 1.088 0.998 SPILLED 1.073 1.027 1.077 1.108 1.016 1.143 1.038 NOT TAKEN 1.173 0.958 0.994 NOT TAKEN 1.137 1.035 1.037 1.204 1.047 0.951 NOT TAKEN 1.055 1.057 1.013 0.972 1.138 1.026 1.042 1.082 0.947 1.033 0.908 0.910 1.032 0.951 1.076 1.058 1.015 1.097 1.012 NOT TAKEN 1.232 1.015 0.968 NOT TAKEN SPILLED 0.921 1.031 SPILLED 1.037 0.920 NOT TAKEN 1.038 1.044 0.991 0.980 1.146 NOT TAKEN 1.038 0.896 0.930 1.025 0.896 0.964 0.999 0.959 0.928 1.055 0.983 NOT TAKEN 0.984 NOT TAKEN 1.201 1.021 1.008 NOT TAKEN SPILLED 1.023 1.011 SPILLED 1.029 0.925 NOT TAKEN 0.985 0.969 0.985 0.952 1.087 NOT TAKEN 1.064 1.048 0.950 1.024 0.900 0.926 1.013 0.991 1.080 1.006 0.963 NOT TAKEN 0.992 NOT TAKEN 1.129 0.988 0.997 NOT TAKEN 1.171 0.981 1.010 1.133 1.079 0.919 NOT TAKEN 0.970 1.045 0.963 0.930 1.145 NOT TAKEN 1.015 SPILLED 0.919 1.004 0.891 0.867 1.001 1.003 1.012 1.015 0.895 NOT TAKEN 0.972 NOT TAKEN 1.097 0.911 0.920 NOT TAKEN SPILLED 1.011 0.962 SPILLED 1.069 0.928 NOT TAKEN 0.993 1.071 1.027 0.887 1.040 NOT TAKEN 1.052 1.012 0.926 1.040 0.882 0.930 0.954 0.989 0.945 1.017 0.933 NOT TAKEN 0.915 NOT TAKEN 1.125 0.898 0.845 NOT TAKEN 1.015 0.914 0.875 1.084 1.051 0.806 NOT TAKEN 0.909 1.016 0.888 0.820 1.020 NOT TAKEN 0.992 0.936 0.822 0.912 0.805 0.867 0.884 0.937 0.884 0.904 0.828 NOT TAKEN 0.829 NOT TAKEN 1.051 0.898 0.789 NOT TAKEN 0.817 0.877 0.811 SPILLED 0.924 0.767 NOT TAKEN 0.914 0.972 0.885 0.805 1.003 NOT TAKEN 0.885 0.849 0.810 0.829 0.837 0.812 0.889 0.978 0.749 0.857 0.819 NOT TAKEN 0.798 NOT TAKEN 1.081 0.817 0.760 NOT TAKEN 0.875 0.870 0.797 0.880 0.902 0.754 NOT TAKEN 0.836 1.013 0.870 0.732 0.952 NOT TAKEN 0.854 0.814 SPILLED 0.857 0.783 0.750 0.854 0.940 0.772 0.828 0.795 NOT TAKEN 0.797 NOT TAKEN 1.026 0.817 1458 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 28 ANIMAL NUMBER WEEK 11 WEEK 12 WEEK 13 WEEK 14 WEEK 15 WEEK 16 WEEK 20 WEEK 24 WEEK 28 WEEK 32 GROUP: MALE 5 - 2 0 PPM C92782 C92783 C92784 C92785 C92786 C92787 C92788 C92789 C92790 C92791 C92792 C92793 C92794 C92795 C92796 C92797 C92798 C92799 C92800 C92801 C92802 C92803 C92804 C92805 C92806 C92807 C92808 C92809 C92810 C92811 C92812 1.075 0.999 1.115 1.103 1.061 SPILLED 1.055 SPILLED 1.059 1.079 1.027 NOT TAKEN 1.041 1.089 1.000 1.027 NOT TAKEN 1.066 SPILLED 1.025 1.086 1.020 1.119 1.064 1.021 1.060 1.011 1.114 1.079 1.146 0.959 1.054 0.994 1.126 1.040 1.041 1.152 0.983 1.046 1.035 1.062 1.038 NOT TAKEN 0.998 1.044 0.975 1.035 NOT TAKEN 1.028 1.024 0.985 1.051 0.990 1.078 1.020 1.050 1.013 0.945 1.049 1.055 TECH SPILL 0.950 1.023 0.969 1.037 1.052 0.963 SPILLED 0.985 0.905 1.025 1.047 0.994 NOT TAKEN 1.009 0.967 0.949 0.988 NOT TAKEN 1.062 1.013 0.945 1.049 0.984 1.095 1.010 1.020 0.981 0.968 1.118 1.049 1.046 0.932 1.033 0.957 0.943 0.992 0.961 SPILLED 1.027 NOT TAKEN 1.011 1.040 0.959 NOT TAKEN 0.965 0.998 0.958 1.015 NOT TAKEN NOT TAKEN 0.978 0.920 1.025 0.980 1.104 0.971 0.916 0.977 0.958 1.088 1.037 1.083 NOT TAKEN 1.025 0.930 1.051 0.993 1.014 SPILLED 1.000 NOT TAKEN 0.957 1.042 0.980 NOT TAKEN 0.951 1.017 0.993 1.002 NOT TAKEN NOT TAKEN 0.967 0.970 1.041 0.989 1.053 0.964 0.877 1.035 0.972 1.097 1.056 1.100 NOT TAKEN 0.992 0.953 1.041 0.988 1.021 1.073 0.952 NOT TAKEN 0.882 1.064 1.003 NOT TAKEN 0.930 1.031 0.991 1.011 NOT TAKEN NOT TAKEN 0.958 0.913 1.048 0.994 1.049 0.914 0.937 0.986 0.961 1.055 0.975 1.101 NOT TAKEN 0.915 0.898 0.949 0.961 0.972 1.037 0.971 NOT TAKEN 0.923 1.076 0.947 NOT TAKEN 0.880 0.963 0.902 1.054 NOT TAKEN NOT TAKEN 0.958 0.840 0.987 0.973 1.030 0.903 0.884 0.904 0.930 1.024 0.920 1.034 NOT TAKEN 0.886 0.858 0.889 0.865 0.883 0.984 0.867 NOT TAKEN 0.856 0.974 0.876 NOT TAKEN 0.872 0.861 0.909 0.958 NOT TAKEN NOT TAKEN 0.862 0.862 0.913 0.935 0.950 0.825 0.844 0.837 0.872 0.858 0.881 0.944 NOT TAKEN 0.801 0.843 0.897 0.855 0.859 1.045 0.886 NOT TAKEN 0.843 0.973 0.802 NOT TAKEN 0.779 0.840 0.868 0.892 NOT TAKEN NOT TAKEN 0.854 0.830 0.905 0.938 0.897 0.787 0.891 0.802 0.864 0.865 0.848 0.897 NOT TAKEN 0.772 0.790 0.864 0.809 0.828 1.040 0.825 NOT TAKEN 0.821 0.916 0.817 NOT TAKEN 0.797 0.809 0.872 0.884 NOT TAKEN NOT TAKEN 0.797 0.766 0.889 0.931 0.891 0.750 0.810 0.781 0.825 0.809 0.814 0.840 NOT TAKEN 1459 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 29 ANIMAL NUMBER WEEK 11 WEEK 12 WEEK 13 WEEK 14 WEEK 15 WEEK 16 WEEK 20 WEEK 24 WEEK 28 WEEK 32 GROUP: MALE 5 - 2 0 PPM C92813 C92814 C92815 C92816 C92817 C92818 C92819 C92820 1.129 0.989 SPILLED 1.005 1.026 1.069 1.049 SPILLED 1.017 0.983 SPILLED 0.960 0.997 1.010 0.991 1.617 GROUP: MALE 6 - 2 0 PPM C92821 C92822 C92823 C92824 C92825 C92826 C92827 C92828 C92829 C92830 C92831 C92832 C92833 C92834 C92835 C92836 C92837 C92838 C92839 C92840 1.088 1.042 1.053 1.056 1.253 1.116 1.095 1.006 1.068 1.183 1.080 SPILLED 0.995 1.051 1.043 1.116 0.998 1.068 1.086 1.023 1.147 1.031 1.027 1.032 1.074 1.264 1.082 0.975 1.070 1.196 1.085 1.033 0.964 1.023 1.011 0.984 0.962 1.052 1.076 0.998 1.045 0.954 SPILLED 0.951 0.990 1.009 1.033 1.563 1.092 0.994 0.985 1.011 1.069 1.145 1.111 1.005 1.057 1.204 1.062 1.021 0.966 0.956 0.994 0.944 0.937 1.070 1.072 1.026 1.068 1.027 1.246 0.958 0.969 1.010 1.099 1.194 1.021 1.023 SPILLED 0.929 0.964 1.020 1.048 1.407 1.006 1.082 1.256 0.900 0.947 1.035 1.074 1.286 0.965 0.963 SPILLED 0.862 0.934 0.991 1.051 1.115 0.919 0.883 1.108 0.810 0.829 0.886 0.911 0.953 0.904 0.925 1.129 0.833 0.816 0.838 0.896 1.140 0.934 0.857 1.071 0.737 0.792 0.860 0.884 1.138 1.023 1.052 0.985 1.037 1.080 1.161 1.183 1.046 1.059 1.179 0.905 1.033 0.993 0.992 1.014 1.005 0.917 1.083 1.022 0.987 1.019 1.021 1.002 0.997 1.060 1.110 1.138 1.008 1.043 1.151 1.070 0.978 0.953 0.989 1.044 0.997 0.954 1.011 1.063 0.992 0.946 0.969 1.015 1.019 SPILLED 1.090 1.105 0.966 1.012 1.090 1.072 0.915 0.927 0.988 1.008 0.927 0.880 0.991 1.046 0.974 0.961 1.013 0.811 0.966 1.056 1.158 1.040 0.991 0.978 0.836 0.959 0.928 0.877 0.978 0.962 0.916 0.960 0.958 0.933 0.886 SPILLED 0.864 0.655 0.885 0.927 1.031 0.982 0.911 0.870 0.953 0.901 0.861 0.811 0.930 0.876 0.826 0.848 0.894 0.884 0.862 1.131 0.874 0.868 SPILLED 0.881 0.970 0.892 0.863 0.842 0.925 0.803 0.809 0.752 0.864 0.845 0.831 0.825 0.883 0.818 0.849 0.937 0.791 0.791 SPILLED SPILLED 0.896 0.859 0.820 0.823 0.887 0.762 0.822 0.732 0.839 0.807 0.805 0.841 0.848 0.798 0.824 1460 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 30 ANIMAL NUMBER WEEK 11 WEEK 12 WEEK 13 WEEK 14 WEEK 15 WEEK 16 WEEK 20 WEEK 24 WEEK 28 WEEK 32 GROUP: MALE 6 - 2 0 PPM C92841 C92842 C92843 C92844 C92845 C92846 C92847 C92848 C92849 C92850 C92851 C92852 C92853 C92854 C92855 C92856 C92857 C92858 C92859 C92860 1.087 1.032 1.051 1.071 1.237 1.099 1.091 1.230 SPILLED 1.183 0.989 1.225 1.029 1.096 1.154 1.073 0.955 1.069 1.064 1.130 0.997 0.961 0.988 0.981 1.167 1.062 1.060 1.183 SPILLED 1.166 0.988 1.143 0.982 1.064 1.062 1.014 0.926 1.012 1.037 1.080 GROUP: FEMALE 2 - 0.5 PPM C92931 C92932 C92933 C92934 C92935 C92936 C92937 C92938 0.030 SPILLED 0.036 0.041 0.030 0.029 0.034 0.032 0.028 SPILLED 0.034 SPILLED 0.029 0.032 0.037 SPILLED 0.768 0.965 0.968 1.003 SPILLED 1.034 1.031 1.222 SPILLED SPILLED 0.964 1.085 0.971 1.023 1.094 1.055 0.933 0.995 0.998 1.059 0.029 SPILLED 0.031 0.036 0.028 0.030 0.034 0.045 1.071 0.941 0.920 1.024 1.188 0.953 1.002 SPILLED SPILLED 1.193 0.987 1.023 0.992 1.025 1.484 1.045 0.956 0.963 1.013 1.088 0.026 SPILLED 0.027 SPILLED 0.023 0.028 0.034 SPILLED 1.054 0.847 0.972 1.043 1.120 0.976 1.006 1.118 1.170 1.162 1.036 1.049 0.948 1.025 1.145 1.038 0.946 0.964 1.008 1.057 0.028 SPILLED 0.029 0.034 0.026 0.030 0.034 0.033 0.983 0.936 1.032 1.075 1.120 1.005 0.946 1.071 1.198 SPILLED 1.013 1.099 0.994 1.006 1.067 1.006 0.955 0.923 0.962 1.040 0.028 0.036 0.031 0.034 0.026 0.029 0.033 SPILLED 0.948 0.923 1.024 1.082 1.124 0.946 0.944 1.065 SPILLED SPILLED 0.934 1.010 1.007 1.013 0.879 0.947 0.908 0.913 0.997 1.046 0.027 SPILLED 0.029 0.032 0.027 0.025 0.034 SPILLED 0.848 0.864 0.820 0.978 1.004 0.851 0.868 1.048 1.114 1.079 0.874 0.904 0.898 0.932 0.927 0.897 0.780 0.878 0.963 0.960 0.026 0.040 0.027 0.030 0.025 0.024 0.031 SPILLED 0.846 0.796 0.835 0.964 0.958 SPILLED 0.808 0.959 SPILLED SPILLED 0.879 0.898 0.876 0.907 0.922 0.877 0.763 0.815 0.883 0.880 0.025 0.041 0.025 0.028 0.021 0.026 0.030 0.025 0.790 0.788 0.760 0.854 0.921 0.888 0.782 0.891 SPILLED SPILLED 0.981 0.960 0.858 0.811 0.835 0.854 0.728 SPILLED 0.874 0.831 0.024 0.054 0.024 0.027 0.025 0.024 0.028 SPILLED 1461 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 31 ANIMAL NUMBER WEEK 11 WEEK 12 WEEK 13 WEEK 14 WEEK 15 WEEK 16 WEEK 20 WEEK 24 WEEK 28 WEEK 32 GROUP: C92939 C92940 C92941 C92942 C92943 C92944 C92945 C92946 C92947 C92948 C92949 C92950 C92951 C92952 C92953 C92954 C92955 C92956 C92957 C92958 C92959 C92960 C92961 C92962 C92963 C92964 C92965 C92966 C92967 C92968 C92969 FEMALE 2 - 0.5 PPM 0 .035 SPILLED NOT TAKEN 0 .033 0 .029 0 .034 NOT TAKEN 0 .031 0 .034 0 .031 0 .027 NOT TAKEN 0 .029 0 .033 0 .033 0 .034 0 .033 0 .032 0 .035 0 .033 0 .035 0 .031 0 .032 0 .032 0 .033 0 .032 0 .033 0 .032 0 .030 0 .030 NOT TAKEN SPILLED SPILLED NOT TAKEN SPILLED 0.031 SPILLED NOT TAKEN 0.031 0.031 0.028 0.030 NOT TAKEN SPILLED 0.030 0.031 0.035 0.030 0.027 0.033 0.032 0.032 0.036 0.030 0.031 0.036 SPILLED 0.034 0.033 SPILLED 0.030 NOT TAKEN SPILLED SPILLED NOT TAKEN 0.034 0.032 SPILLED NOT TAKEN 0.031 0.032 0.031 0.031 NOT TAKEN SPILLED 0.030 0.032 SPILLED 0.030 0.027 SPILLED 0.032 0.035 0.035 0.029 0.031 0.032 0.031 0.031 0.034 0.029 0.031 NOT TAKEN SPILLED SPILLED NOT TAKEN SPILLED 0.035 NOT TAKEN NOT TAKEN 0.027 0.023 0.025 0.029 NOT TAKEN 0.031 0.033 0.030 0.034 0.029 0.022 SPILLED 0.028 0.034 0.032 0.028 NOT TAKEN 0.034 SPILLED 0.030 0.032 NOT TAKEN 0.028 NOT TAKEN 0 .044 0 .047 NOT TAKEN 0 .033 0 .032 NOT TAKEN NOT TAKEN 0 .030 0 .029 0 .030 0 .027 NOT TAKEN 0 .031 0 .031 0 .028 0 .032 0 .029 0 .028 0 .034 0 .031 0 .030 0 .033 0 .028 NOT TAKEN 0 .031 0 .031 0 .031 0 .031 NOT TAKEN 0 .028 NOT TAKEN SPILLED 0 .036 NOT TAKEN SPILLED 0 .030 NOT TAKEN NOT TAKEN 0 .031 0 .030 0 .028 0 .029 NOT TAKEN 0 .032 0 .030 0 .031 0 .032 0 .029 0 .028 0 .033 0 .033 0 .032 0 .032 0 .027 NOT TAKEN 0 .031 0 .050 0 .031 0 .033 NOT TAKEN 0 .028 NOT TAKEN SPILLED 0 .033 NOT TAKEN 0 .031 0 .027 NOT TAKEN NOT TAKEN 0 .025 0 .030 0 .029 0 .026 NOT TAKEN 0 .033 0 .030 0 .031 0 .030 0 .028 0 .028 0 .032 0 .033 0 .033 0 .032 0 .025 NOT TAKEN 0 .031 SPILLED 0 .030 0 .033 NOT TAKEN 0 .027 NOT TAKEN SPILLED 0.030 NOT TAKEN 0.029 0.023 NOT TAKEN NOT TAKEN 0.028 0.023 0.029 0.024 NOT TAKEN SPILLED 0.029 0.027 0.027 0.028 0.024 SPILLED 0.029 0.031 SPILLED 0.024 NOT TAKEN 0.027 SPILLED 0.031 0.028 NOT TAKEN 0.025 NOT TAKEN 0 .032 0 .028 NOT TAKEN 0 .029 0 .025 NOT TAKEN NOT TAKEN 0 .024 0 .024 0 .025 0 .020 NOT TAKEN SPILLED 0 .027 0 .026 0 .029 0 .027 0 .025 0 .029 0 .027 0 .044 0 .035 0 .023 NOT TAKEN 0 .030 0 .029 CNTMD 0 .027 NOT TAKEN 0 .025 NOT TAKEN SPILLED 0.028 NOT TAKEN 0.028 0.027 NOT TAKEN NOT TAKEN 0.025 0.024 0.023 0.014 NOT TAKEN 0.028 0.025 0.029 0.028 0.024 0.024 0.026 0.028 0.027 0.027 0.021 NOT TAKEN 0.029 0.033 0.031 0.024 NOT TAKEN 0.020 NOT TAKEN 1462 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 32 ANIMAL NUMBER WEEK 11 WEEK 12 WEEK 13 WEEK 14 WEEK 15 WEEK 16 WEEK 20 WEEK 24 WEEK 28 WEEK 32 GROUP: FEMALE 2 - 0 . 5 PPM C92970 C92971 C92972 C92973 C92974 C92975 C92976 C92977 C92978 C92979 C92980 C92981 C92982 C92983 C92984 C92985 C92986 C92987 C92988 C92989 C92990 0 .030 0 .031 0 .036 0 .037 0 .032 0 .037 0 .030 0 .035 0 .031 0 .034 0 .034 0 .031 NOT TAKEN 0 .033 0 .031 0 .031 0 .034 0 .030 0 .035 SPILLED 0 .037 0 .032 0 .032 0 .037 0 .036 0 .030 0 .036 0 .030 0 .041 0 .031 0 .029 0 .032 SPILLED NOT TAKEN 0 .032 0 .029 0 .031 0 .032 0 .031 0 .031 SPILLED 0 .036 GROUP: FEMALE: 3 - 2 PPM C92991 C92992 C92993 C92994 C92995 C92996 C92997 0 .127 0 .137 0 .129 0 .127 NOT TAKEN NOT TAKEN 0 .134 0 .123 0 .126 0 .127 0 .134 NOT TAKEN NOT TAKEN 0 .128 0.031 0.030 0.035 SPILLED 0.030 0.035 0.027 0.036 0.029 0.032 0.031 0.030 NOT TAKEN 0.032 0.029 0.032 0.033 0.031 0.035 SPILLED 0.034 0.119 0.125 0.120 0.119 NOT TAKEN NOT TAKEN 0.123 0.030 0.032 0.032 0.038 0.034 0.034 0.026 0.033 NOT TAKEN 0.030 0.030 0.030 NOT TAKEN SPILLED 0.027 0.028 0.029 NOT TAKEN 0.036 SPILLED 0.036 0.125 SPILLED NOT TAKEN 0.115 NOT TAKEN NOT TAKEN SPILLED 0.031 0.028 0.034 0.037 0.032 0.033 0.027 0.031 NOT TAKEN 0.029 0.029 0.029 NOT TAKEN 0.027 0.027 0.028 0.033 NOT TAKEN 0.033 0.038 0.034 0.119 0.139 NOT TAKEN 0.131 NOT TAKEN NOT TAKEN 0.127 0.031 0.030 0.037 0.036 0.031 0.035 0.028 SPILLED NOT TAKEN 0.028 0.028 0.027 NOT TAKEN 0.028 0.026 SPILLED 0.033 NOT TAKEN 0.031 SPILLED 0.033 0.126 0.134 NOT TAKEN 0.122 NOT TAKEN NOT TAKEN SPILLED 0.027 0.029 0.033 0.034 0.029 0.035 0.028 0.029 NOT TAKEN 0.025 0.029 0.027 NOT TAKEN 0.035 0.028 0.029 0.031 NOT TAKEN 0.029 0.037 0.030 0.123 0.133 NOT TAKEN 0.122 NOT TAKEN NOT TAKEN SPILLED 0.025 0.026 0.030 0.038 0.026 0.030 0.028 0.027 NOT TAKEN 0.028 0.027 0.031 NOT TAKEN 0.026 0.025 0.027 0.028 NOT TAKEN 0.028 0.036 0.030 0.115 0.128 NOT TAKEN 0.106 NOT TAKEN NOT TAKEN 0.124 0.025 0.030 0.028 0.030 0.024 0.030 0.025 0.026 NOT TAKEN 0.026 0.021 0.023 NOT TAKEN 0.026 0.024 0.024 0.027 NOT TAKEN 0.031 0.044 0.031 0.129 0.127 NOT TAKEN 0.106 NOT TAKEN NOT TAKEN 0.129 0.023 0.027 0.028 0.032 0.024 0.027 0.023 0.027 NOT TAKEN 0.023 0.030 0.027 NOT TAKEN 0.026 0.022 0.028 0.026 NOT TAKEN 0.025 SPILLED 0.041 0.120 0.126 NOT TAKEN 0.102 NOT TAKEN NOT TAKEN 0.120 1463 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 33 ANIMAL NUMBER WEEK 11 WEEK 12 WEEK 13 WEEK 14 WEEK 15 WEEK 16 WEEK 20 WEEK 24 WEEK 28 WEEK 32 GROUP: FEMALE 3 - 2 PPM C92998 C92999 C93000 C93001 C93002 C93003 C93004 C93005 C93006 C93007 C93008 C93009 C93010 C93011 C93012 C93013 C93014 C93015 C93016 C93017 C93018 C93019 C93020 C93021 C93022 C93023 C93024 C93025 C93026 C93027 C93028 0.143 SPILLED 0.129 0.141 0.137 0.109 0.132 0.133 NOT TAKEN 0.132 NOT TAKEN SPILLED 0.152 0.125 0.132 0.138 0.123 0.128 0.128 0.152 0.135 0.132 0.132 0.132 0.125 SPILLED 0.144 0.147 0.142 0.127 0.139 0.139 SPILLED 0.148 0.146 0.143 0.123 0.133 0.133 NOT TAKEN SPILLED NOT TAKEN 0.171 0.147 0.117 0.120 0.147 0.140 SPILLED SPILLED 0.148 0.127 SPILLED 0.196 0.114 0.112 SPILLED 0.141 0.146 0.137 0.136 0.128 0.135 0.134 0.123 0.133 0.130 0.118 0.125 0.138 NOT TAKEN 0.115 NOT TAKEN 0.128 0.139 0.110 0.121 0.124 0.122 SPILLED 0.143 0.147 0.123 SPILLED 0.134 0.119 0.112 SPILLED 0.128 0.137 0.122 0.128 SPILLED 0.136 SPILLED NOT TAKEN 0.129 0.133 0.114 0.123 0.146 NOT TAKEN 0.129 NOT TAKEN 0.139 SPILLED 0.115 0.122 0.145 0.116 SPILLED 0.142 0.149 NOT TAKEN SPILLED 0.146 0.121 0.119 NOT TAKEN 0.128 0.132 0.108 0.154 SPILLED 0.135 0.150 NOT TAKEN 0.132 0.118 0.121 0.126 0.141 NOT TAKEN 0.132 NOT TAKEN 0.122 0.145 0.113 0.128 0.124 0.124 0.128 SPILLED 0.137 NOT TAKEN SPILLED 0.132 0.108 0.106 NOT TAKEN 0.143 0.141 0.120 0.118 SPILLED 0.139 SPILLED NOT TAKEN 0.131 0.133 0.111 0.120 0.128 NOT TAKEN 0.118 NOT TAKEN 0.123 0.169 0.118 0.124 0.133 0.112 SPILLED 0.171 0.135 NOT TAKEN 0.127 0.126 0.117 0.112 NOT TAKEN 0.129 0.104 0.120 0.120 0.131 0.123 SPILLED NOT TAKEN 0.139 0.131 0.127 0.112 0.149 NOT TAKEN 0.116 NOT TAKEN 0.117 0.130 0.103 0.121 0.115 0.113 SPILLED SPILLED 0.128 NOT TAKEN 0.123 0.128 0.128 0.103 NOT TAKEN 0.119 0.129 0.127 0.115 SPILLED 0.124 0.109 NOT TAKEN 0.145 0.119 0.108 0.105 0.133 NOT TAKEN 0.114 NOT TAKEN 0.122 0.117 0.099 0.114 0.142 0.093 SPILLED SPILLED 0.112 NOT TAKEN SPILLED 0.132 0.110 0.099 NOT TAKEN 0.118 0.109 0.124 0.114 0.120 0.125 0.117 NOT TAKEN 0.142 0.124 0.123 0.094 0.116 NOT TAKEN 0.112 NOT TAKEN 0.139 0.110 0.096 0.114 0.108 0.107 0.111 0.206 0.120 NOT TAKEN 0.110 0.122 0.102 0.092 NOT TAKEN 0.118 0.119 0.118 0.108 SPILLED 0.106 0.112 NOT TAKEN 0.121 0.120 0.094 0.101 0.116 NOT TAKEN 0.101 NOT TAKEN 0.116 0.124 0.105 0.106 0.110 0.105 0.122 0.167 0.114 NOT TAKEN 0.105 0.096 0.098 0.099 NOT TAKEN 0.110 0.103 0.120 0.104 SPILLED 1464 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 34 ANIMAL NUMBER WEEK 11 WEEK 12 WEEK 13 WEEK 14 WEEK 15 WEEK 16 WEEK 20 WEEK 24 WEEK 28 WEEK 32 GROUP: FEMALE 3 - 2 PPM C93029 C93030 C93031 C93032 C93033 C93034 C93035 C93036 C93037 C93038 C93039 C93040 C93041 C93042 C93043 C93044 C93045 C93046 C93047 C93048 C93049 C93050 SPILLED 0.146 NOT TAKEN 0.131 SPILLED 0.147 0.142 SPILLED 0.133 SPILLED 0.146 SPILLED 0.165 0.133 0.145 0.140 NOT TAKEN 0.107 0.134 0.154 0.137 0.145 SPILLED SPILLED NOT TAKEN 0.138 SPILLED SPILLED 0.134 0.131 0.134 SPILLED 0.134 SPILLED SPILLED 0.132 0.150 0.126 NOT TAKEN 0.156 0.128 SPILLED 0.133 SPILLED GROUP: FEMALE 4 - 5 PPM C93051 C93052 C93053 C93054 C93055 C93056 0.351 SPILLED 0.385 SPILLED SPILLED 0.321 0.397 SPILLED 0.345 SPILLED 0.471 0.322 SPILLED 0.131 NOT TAKEN 0.128 SPILLED 0.133 0.125 0.121 0.127 0.149 0.134 SPILLED 0.158 0.125 0.142 0.122 NOT TAKEN 0.135 0.117 0.155 0.131 0.151 0.358 SPILLED 0.332 0.358 0.328 0.321 SPILLED 0.119 NOT TAKEN 0.117 SPILLED 0.123 NOT TAKEN SPILLED 0.128 0.148 0.127 0.151 SPILLED 0.133 0.132 0.129 NOT TAKEN 0.123 0.121 0.144 0.129 SPILLED NOT TAKEN SPILLED 0.357 0.379 SPILLED 0.305 SPILLED 0.142 NOT TAKEN 0.124 SPILLED 0.119 NOT TAKEN 0.127 0.124 0.149 0.119 SPILLED 0.137 0.115 0.142 0.130 NOT TAKEN 0.125 0.124 0.144 0.126 0.140 NOT TAKEN SPILLED 0.330 0.337 0.312 0.311 SPILLED SPILLED NOT TAKEN 0.130 SPILLED 0.127 NOT TAKEN 0.109 0.146 0.153 0.112 SPILLED 0.143 0.101 0.131 SPILLED NOT TAKEN 0.145 0.117 0.162 0.129 0.154 NOT TAKEN SPILLED 0.366 SPILLED 0.307 0.311 SPILLED 0.125 NOT TAKEN 0.127 SPILLED 0.107 NOT TAKEN 0.117 0.104 0.135 0.112 0.131 0.148 0.143 0.119 0.122 NOT TAKEN 0.124 0.106 0.144 0.122 0.137 NOT TAKEN SPILLED 0.326 0.331 0.300 0.322 SPILLED 0.121 NOT TAKEN 0.119 0.159 0.117 NOT TAKEN 0.116 0.101 0.132 0.109 0.155 0.147 0.126 0.129 SPILLED NOT TAKEN 0.126 0.093 0.137 0.112 0.138 NOT TAKEN SPILLED 0.301 0.325 0.268 0.293 0.165 0.104 NOT TAKEN 0.116 SPILLED 0.116 NOT TAKEN 0.103 0.108 0.121 0.108 0.161 0.129 0.122 0.111 0.178 NOT TAKEN TECH SPILL 0.099 SPILLED 0.114 0.126 SPILLED 0.111 NOT TAKEN 0.110 0.121 0.113 NOT TAKEN 0.090 0.100 0.121 0.100 0.142 0.115 0.105 0.115 SPILLED NOT TAKEN SPILLED 0.107 0.125 0.108 0.124 NOT TAKEN 0.282 0.272 0.320 0.286 0.314 NOT TAKEN SPILLED 0.262 0.307 0.296 0.299 1465 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 35 ANIMAL NUMBER WEEK 11 WEEK 12 WEEK 13 WEEK 14 WEEK 15 WEEK 16 WEEK 20 WEEK 24 WEEK 28 WEEK 32 GROUP: FEMALE 4 - 5 PPM C93057 C93058 C93059 C93060 C93061 C93062 C93063 C93064 C93065 C93066 C93067 C93068 C93069 C93070 C93071 C93072 C93073 C93074 C93075 C93076 C93077 C93078 C93079 C93080 C93081 C93082 C93083 C93084 C93085 C93086 C93087 0.366 NOT TAKEN 0.348 0.360 0.348 0.347 0.319 0.372 0.380 0.367 NOT TAKEN 0.336 0.313 NOT TAKEN 0.340 0.420 0.371 0.431 NOT TAKEN SPILLED 0.324 0.360 0.345 0.343 0.323 0.295 0.343 NOT TAKEN 0.345 0.314 0.362 0.359 NOT TAKEN 0.346 0.345 0.296 0.327 0.293 0.340 0.417 0.333 NOT TAKEN 0.338 0.307 NOT TAKEN 0.329 0.413 0.365 SPILLED NOT TAKEN 0.322 0.300 0.455 0.361 0.335 0.329 0.295 0.342 NOT TAKEN 0.338 0.373 0.364 0.362 NOT TAKEN 0.320 0.313 0.314 0.319 0.324 0.331 0.360 0.326 NOT TAKEN 0.315 0.306 NOT TAKEN 0.316 SPILLED 0.363 0.361 NOT TAKEN SPILLED 0.288 SPILLED 0.351 0.317 0.332 0.294 0.349 NOT TAKEN SPILLED 0.303 0.303 0.351 NOT TAKEN 0.325 0.319 0.298 0.310 0.297 NOT TAKEN 0.368 0.321 NOT TAKEN 0.314 0.289 NOT TAKEN NOT TAKEN 0.348 0.336 SPILLED NOT TAKEN 0.260 0.270 SPILLED 0.330 0.296 0.303 0.285 0.336 NOT TAKEN NOT TAKEN SPILLED 0.314 0.353 NOT TAKEN 0.315 0.319 0.319 0.314 0.306 NOT TAKEN 0.356 0.330 NOT TAKEN 0.309 0.278 NOT TAKEN NOT TAKEN SPILLED 0.349 0.351 NOT TAKEN SPILLED 0.262 0.353 0.366 0.320 0.319 0.291 0.346 NOT TAKEN NOT TAKEN 0.309 0.313 0.325 NOT TAKEN 0.338 0.296 0.304 0.305 0.290 NOT TAKEN 0.367 0.317 NOT TAKEN 0.310 0.280 NOT TAKEN NOT TAKEN 0.349 0.357 0.369 NOT TAKEN SPILLED 0.266 0.366 0.316 0.293 0.288 0.304 0.334 NOT TAKEN NOT TAKEN 0.306 0.326 0.312 NOT TAKEN 0.307 0.277 0.321 0.308 0.265 NOT TAKEN 0.340 SPILLED NOT TAKEN 0.294 0.286 NOT TAKEN NOT TAKEN 0.348 0.335 0.357 NOT TAKEN 0.306 0.299 0.401 0.311 0.384 0.304 0.305 0.305 NOT TAKEN NOT TAKEN 0.305 0.317 0.322 NOT TAKEN 0.278 0.279 0.312 0.293 0.251 NOT TAKEN 0.323 SPILLED NOT TAKEN 0.296 0.298 NOT TAKEN NOT TAKEN 0.322 0.316 0.303 NOT TAKEN 0.287 0.277 0.306 0.264 0.311 0.277 0.316 0.294 NOT TAKEN NOT TAKEN 0.268 0.283 0.304 NOT TAKEN 0.267 0.249 0.296 0.298 0.251 NOT TAKEN 0.304 0.335 NOT TAKEN 0.250 0.251 NOT TAKEN NOT TAKEN 0.318 0.306 0.272 NOT TAKEN 0.273 0.263 0.309 0.277 SPILLED 0.304 0.265 0.294 NOT TAKEN NOT TAKEN 0.271 0.273 0.304 NOT TAKEN 0.280 0.270 0.278 SPILLED 0.260 NOT TAKEN 0.272 SPILLED NOT TAKEN 0.284 0.261 NOT TAKEN NOT TAKEN 0.293 0.288 0.242 NOT TAKEN 0.235 0.252 0.310 0.253 SPILLED 0.266 0.261 0.267 NOT TAKEN NOT TAKEN 0.248 0.297 1466 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 36 ANIMAL NUMBER WEEK 11 WEEK 12 WEEK 13 WEEK 14 WEEK 15 WEEK 16 WEEK 20 WEEK 24 WEEK 28 WEEK 32 GROUP: FEMALE 4 - 5 PPM C93088 C93089 C93090 C93091 C93092 C93093 C93094 C93095 C93096 C93097 C93098 C93099 C93100 C93101 C93102 C93103 C93104 C93105 C93106 C93107 C93108 C93109 C93110 0.300 0.355 0.345 0.303 0.365 0.293 0.345 0.337 0.345 SPILLED 0.332 0.322 0.399 0.326 SPILLED 0.346 SPILLED 0.337 0.338 0.325 0.324 0.351 0.379 0.359 0.317 0.311 0.308 0.350 0.292 0.349 SPILLED 0.361 SPILLED 0.351 0.289 0.374 0.300 SPILLED 0.324 0.404 0.346 0.307 0.300 SPILLED 0.334 0.362 GROUP: FEMALE 5 - 20 PPM C93111 C93112 C93113 C93114 C93115 1.235 1.298 1.511 NOT TAKEN 1.445 1.190 1.219 1.411 NOT TAKEN 1.486 0.326 0.296 0.316 0.288 0.307 0.335 0.310 0.349 0.343 SPILLED 0.309 0.301 0.381 0.267 0.329 0.335 0.355 0.318 0.298 0.275 0.298 0.291 0.366 1.065 1.118 1.276 NOT TAKEN 1.406 0.335 0.268 0.284 0.298 0.322 0.237 0.304 0.316 SPILLED SPILLED 0.349 0.283 0.346 0.290 SPILLED 0.302 SPILLED 0.290 0.292 0.283 0.282 NOT TAKEN SPILLED NOT TAKEN 1.208 1.315 NOT TAKEN 1.395 0.323 0.314 0.334 0.286 0.329 0.322 0.319 0.317 0.323 SPILLED 0.297 0.280 0.353 0.300 0.335 0.286 SPILLED 0.299 0.313 0.289 0.298 NOT TAKEN 0.326 NOT TAKEN 1.149 1.498 NOT TAKEN 1.331 0.340 0.295 0.298 0.267 0.320 0.331 0.334 0.316 0.311 SPILLED 0.308 0.291 0.369 0.285 0.331 0.290 0.322 0.299 0.295 0.286 0.300 NOT TAKEN SPILLED NOT TAKEN 1.197 1.420 NOT TAKEN 1.346 0.327 0.310 0.292 0.295 0.366 SPILLED 0.313 0.302 0.320 SPILLED 0.312 0.260 0.376 SPILLED 0.347 0.318 0.305 0.298 0.310 0.285 SPILLED NOT TAKEN 0.291 NOT TAKEN SPILLED 1.347 NOT TAKEN 1.323 0.300 0.280 0.262 0.278 0.315 SPILLED 0.267 0.255 0.296 0.329 0.286 0.292 0.316 0.257 0.302 0.212 0.285 0.261 0.289 0.250 0.275 NOT TAKEN 0.288 NOT TAKEN 1.114 1.249 NOT TAKEN 1.271 0.314 0.265 0.262 0.281 0.311 0.220 0.276 0.251 0.250 0.304 0.254 0.277 0.284 0.264 SPILLED 0.288 0.255 0.248 0.294 0.253 0.244 NOT TAKEN 0.282 NOT TAKEN 1.123 1.191 NOT TAKEN 1.150 0.293 0.279 0.247 0.231 0.325 0.265 0.275 0.242 NOT TAKEN 0.289 0.266 0.270 0.290 0.253 0.302 0.372 0.243 0.252 0.272 0.254 0.242 NOT TAKEN 0.223 NOT TAKEN 1.093 1.231 NOT TAKEN 1.087 1467 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 37 ANIMAL NUMBER WEEK 11 WEEK 12 WEEK 13 WEEK 14 WEEK 15 WEEK 16 WEEK 20 WEEK 24 WEEK 28 WEEK 32 GROUP: FEMALE 5 - 2 0 PPM C93116 C93117 C93118 C93119 C93120 C93121 C93122 C93123 C93124 C93125 C93126 C93127 C93128 C93129 C93130 C93131 C93132 C93133 C93134 C93135 C93136 C93137 C93138 C93139 C93140 C93141 C93142 C93143 C93144 C93145 C93146 SPILLED 1.326 1.416 1.455 1.276 1.321 1.263 NOT TAKEN 1.451 1.533 1.356 1.366 1.374 1.481 1.434 1.338 1.347 1.369 1.240 1.349 1.420 1.453 1.175 1.575 1.538 1.448 NOT TAKEN SPILLED NOT TAKEN 1.673 1.370 1.405 1.374 1.373 1.442 1.491 1.386 1.280 NOT TAKEN 1.404 1.581 1.224 1.429 1.454 1.382 1.493 1.202 1.257 1.385 1.238 1.381 1.434 1.497 1.310 1.497 1.473 1.389 NOT TAKEN 1.824 NOT TAKEN 1.488 1.363 1.259 1.173 1.381 1.351 1.370 1.286 0.968 NOT TAKEN 1.441 1.562 1.182 1.252 1.385 1.336 1.332 1.202 1.192 1.246 1.131 1.366 1.298 1.443 1.223 1.471 1.321 1.434 NOT TAKEN 1.813 NOT TAKEN SPILLED 1.373 1.232 1.257 1.417 1.317 1.204 1.257 1.202 NOT TAKEN 1.086 1.579 1.277 NOT TAKEN 1.316 1.394 1.321 1.196 1.256 1.420 1.199 1.195 1.377 1.513 1.193 1.483 1.371 1.287 NOT TAKEN NOT TAKEN NOT TAKEN 1.593 SPILLED 1.311 SPILLED 1.417 1.378 1.443 1.273 1.199 NOT TAKEN 1.113 1.469 1.292 NOT TAKEN 1.295 1.283 1.382 1.172 1.287 1.386 1.176 1.452 1.410 1.483 1.194 1.429 1.470 1.342 NOT TAKEN NOT TAKEN NOT TAKEN 1.750 1.224 1.282 1.258 1.498 1.266 1.257 1.261 1.197 NOT TAKEN 1.277 1.441 1.284 NOT TAKEN 1.306 1.466 1.367 SPILLED 1.286 1.457 1.156 1.299 1.405 1.409 1.213 1.378 1.464 1.491 NOT TAKEN NOT TAKEN NOT TAKEN 1.755 1.316 1.265 SPILLED 1.401 1.303 1.355 1.319 1.185 NOT TAKEN 1.218 1.499 1.315 NOT TAKEN 1.265 1.544 1.317 1.168 1.385 SPILLED 1.220 1.168 1.410 1.467 1.234 SPILLED 1.376 1.301 NOT TAKEN NOT TAKEN NOT TAKEN 1.423 1.458 1.280 1.190 1.258 1.190 1.291 1.215 1.076 NOT TAKEN 1.279 1.234 1.219 NOT TAKEN 1.153 1.604 1.208 1.157 1.178 SPILLED 1.054 1.049 1.419 1.301 1.143 1.235 1.233 1.168 NOT TAKEN NOT TAKEN NOT TAKEN SPILLED 1.165 1.177 1.232 0.955 1.228 1.229 1.134 1.152 NOT TAKEN 1.055 1.232 1.223 NOT TAKEN 1.039 1.177 1.022 1.121 1.109 SPILLED 1.058 1.154 1.324 1.224 1.106 1.234 1.091 1.214 NOT TAKEN NOT TAKEN NOT TAKEN 1.778 0.993 1.231 1.209 1.197 1.202 1.196 1.101 1.180 NOT TAKEN 0.985 1.198 1.189 NOT TAKEN 1.219 1.270 1.212 1.095 1.068 1.200 0.982 1.312 1.317 1.185 1.001 1.242 1.135 1.078 NOT TAKEN NOT TAKEN NOT TAKEN SPILLED 1.129 1468 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 38 ANIMAL NUMBER WEEK 11 WEEK 12 WEEK 13 WEEK 14 WEEK 15 WEEK 16 WEEK 20 WEEK 24 WEEK 28 WEEK 32 GROUP: FEMALE 5 - 20 PPM C93147 C93148 C93149 C93150 C93151 C93152 C93153 C93154 C93155 C93156 C93157 C93158 C93159 C93160 C93161 C93162 C93163 C93164 C93165 C93166 C93167 C93168 C93169 C93170 C93171 C93172 C93173 C93174 C93175 C93176 C93177 1.237 1.399 1.392 SPILLED 1.336 1.331 SPILLED 1.303 SPILLED 1.323 1.487 1.312 NOT TAKEN 1.327 1.352 SPILLED 1.269 1.332 1.233 1.214 1.285 1.257 1.408 SPILLED SPILLED 1.289 1.309 SPILLED 1.294 1.294 SPILLED 1.380 1.332 SPILLED 1.611 1.319 1.366 2.495 1.266 SPILLED 1.267 1.519 1.305 NOT TAKEN 1.445 1.293 SPILLED 1.293 1.372 1.313 1.282 1.276 SPILLED 1.380 1.834 SPILLED 1.314 1.288 SPILLED 1.417 1.304 SPILLED 1.297 1.385 SPILLED 1.453 1.260 1.299 SPILLED 1.299 2.449 1.249 1.329 1.330 NOT TAKEN 1.346 SPILLED 1.266 1.269 11..232215 1.163 1.233 1.340 1.310 1.326 1.218 1.389 SPILLED SPILLED 1.233 1.281 1.315 1.277 1.239 1.614 1.275 1.252 1.328 SPILLED 1.224 NOT TAKEN 1.205 1.299 1.302 NOT TAKEN 1.317 1.183 1.030 1.245 1.234 1.283 1.087 1.252 1.383 NOT TAKEN SPILLED SPILLED SPILLED 1.173 SPILLED 1.281 1.271 1.256 1.308 1.285 1.303 1.506 1.285 1.349 SPILLED 1.270 NOT TAKEN 1.228 1.350 1.270 NOT TAKEN 1.311 11..231618 1.223 1.328 1.256 1.255 1.224 1.343 NOT TAKEN 1.714 1.400 1.257 1.228 SPILLED 11..220418 1.336 1.301 1.277 1.269 1.313 1.243 1.266 SPILLED 1.241 NOT TAKEN 1.211 1.322 1.318 NOT TAKEN 11..221624 SPILLED 1.200 1.375 11..120305 1.214 1.414 NOT TAKEN 11..252126 1.245 1.227 SPILLED 1.243 1.237 1.301 1.291 1.278 1.273 1.398 1.334 SPILLED SPILLED 1.184 NOT TAKEN 1.194 1.383 1.337 NOT TAKEN 1.302 1.227 SPILLED 1.573 1.262 1.195 11..112915 SPILLED NOT TAKEN SPILLED 1.143 1.278 1.156 SPILLED 1.235 1.275 SPILLED 1.213 1.261 1.125 1.319 1.274 SPILLED 1.312 1.106 NOT TAKEN 1.142 1.212 1.278 NOT TAKEN 1.136 1.117 SPILLED 1.375 11..122407 1.179 1.118 1.078 NOT TAKEN SPILLED 1.087 1.324 1.352 SPILLED 1.183 1.182 1.573 1.125 1.150 1.098 1.320 1.161 SPILLED 1.438 1.081 NOT TAKEN 1.120 1.291 1.227 NOT TAKEN 1.336 1.113 1.162 1.088 1.175 1.081 SPILLED 1.030 1.141 NOT TAKEN 1.992 1.117 1.218 1.133 SPILLED 1.064 1.104 1.191 1.107 1.092 1.133 1.236 1.130 1.231 1.176 1.141 NOT TAKEN 1.142 1.188 1.193 NOT TAKEN 1.308 1.087 1.196 1.068 1.164 1.092 0.977 0.946 1.149 NOT TAKEN 1.671 1.151 1.067 0.998 SPILLED 1.104 1.028 1.146 1469 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104 -WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 11 WEEK 12 WEEK 13 WEEK 14 WEEK 15 WEEK 16 WEEK 20 WEEK 24 GROUP: C93178 C93179 C93180 GROUP: C93181 C93182 C93183 C93184 C93185 C93186 C93187 C93188 C93189 C93190 C93191 C93192 C93193 C93194 C93195 C93196 C93197 C93198 C93199 C93200 C93201 C93202 C93203 C93204 C93205 FEMALE 5 - 20 PPM 1.304 1.485 1.218 1.306 1.506 1.288 FEMALE 6 - 20 PPM 1.431 1.220 1.248 1.383 1.339 1.359 1.469 1.293 1.443 1.396 1.165 1.471 1.392 1.523 1.257 1.488 1.317 1.294 1.324 SPILLED 1.338 1.257 1.325 1.451 1.672 1.412 1.254 1.250 1.365 1.509 1.322 1.354 1.271 1.393 1.399 1.172 1.492 1.384 1.556 1.229 1.366 1.438 1.453 1.320 SPILLED 1.629 1.324 1.321 1.384 1.297 1.347 1.571 1.285 1.467 1.355 1.264 1.345 SPILLED 1.336 1.362 1.311 1.355 1.293 1.168 1.358 1.379 1.536 1.224 1.429 1.316 1.415 1.292 SPILLED 1.497 SPILLED 1.204 1.395 1.249 1.161 1.274 1.309 1.347 1.236 1.210 1.331 1.416 1.501 1.309 1.222 1.556 1.322 1.183 1.321 1.429 SPILLED 1.197 1.510 1.347 1.313 1.186 SPILLED 1.364 SPILLED 1.403 1.415 1.286 1.412 1.328 1.400 1.309 1.517 1.280 1.344 1.352 1.372 1.162 1.172 1.203 1.346 1.253 1.261 1.361 1.322 1.366 1.383 1.227 1.304 1.338 1.156 1.388 1.302 1.447 1.196 1.411 1.322 1.286 1.199 2.005 1.417 1.202 1.490 1.328 1.319 1.308 1.143 1.159 1.372 1.459 1.379 SPILLED 1.343 1.417 1.255 1.206 1.385 1.376 1.612 1.141 1.417 1.213 1.277 1.201 SPILLED SPILLED 1.669 1.324 1.324 1.177 1.423 1.318 1.187 1.415 1.379 1.485 1.543 1.347 1.416 1.270 1.231 SPILLED 1.409 SPILLED SPILLED 1.376 SPILLED 1.328 1.236 1.592 SPILLED 1.272 1.528 1.322 1.239 1.284 1.105 1.095 1.374 1.373 1.225 1.271 1.248 1.275 1.215 1.178 1.201 1.339 1.051 1.102 1.380 SPILLED 1.151 1.124 1.381 SPILLED 1.133 1.213 1.200 1.084 Covance 6329-183 3M T-6295 PAGE: 39 WEEK 28 WEEK 32 1.096 1.219 1.115 1.221 1.056 1.046 1.268 0.656 1.128 1.154 1.197 1.191 1.186 1.067 1.214 1.142 1.072 1.138 1.184 1.088 1.105 1.094 1.189 1.790 1.058 1.212 1.156 1.029 1.031 1.221 1.094 1.181 1.076 0.939 1.202 1.350 1.295 1.032 1.085 1.252 1.243 1.131 1.241 1.110 1.131 1.103 1.206 1.159 1.151 1.114 1.194 1.665 1.074 1.126 1.147 1.049 1470 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 11 WEEK 12 WEEK 13 WEEK 14 WEEK 15 WEEK 16 WEEK 20 WEEK 24 GROUP: C93206 C93207 C93208 C93209 C93210 C93211 C93212 C93213 C93214 C93215 C93216 C93217 C93218 C93219 C93220 FEMALE 6 - 20 PPM 1.571 1.468 1.440 SPILLED 1.306 1.324 1.600 1.423 1.423 1.291 1.289 1.399 1.666 1.298 1.566 SPILLED 1.597 1.342 SPILLED 1.341 1.374 1.381 1.232 1.365 1.284 1.236 SPILLED 1.621 1.028 SPILLED 1.345 1.458 1.174 SPILLED 1.439 1.297 1.220 1.353 1.323 1.213 1.210 SPILLED 1.614 1.433 1.804 SPILLED 1.307 1.228 SPILLED 1.272 1.342 1.266 1.266 1.364 1.224 1.158 SPILLED SPILLED 1.191 SPILLED 1.461 1.313 1.229 1.308 1.207 1.222 1.343 1.143 1.309 1.200 1.232 SPILLED 1.549 1.135 1.718 SPILLED 1.290 1.186 SPILLED 1.320 1.302 1.325 1.124 1.331 1.155 1.124 1.328 1.466 1.148 SPILLED SPILLED 1.361 1.179 SPILLED SPILLED 1.323 1.338 SPILLED 1.350 1.279 1.258 SPILLED SPILLED 1.254 SPILLED SPILLED 1.194 1.058 SPILLED 1.224 1.138 1.113 SPILLED 1.262 1.116 1.202 SPILLED SPILLED 1.090 SPILLED Covance 6329-183 3M T-6295 PAGE: 40 WEEK 28 WEEK 32 1.285 1.190 1.095 1.702 1.204 1.119 1.083 1.015 1.187 1.040 1.158 1.098 1.342 1.027 1.072 1.221 1.257 1.033 1.795 1.170 1.112 1.150 1.022 1.132 0.995 1.163 1.104 1.106 1.150 1.127 1471 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 41 ANIMAL NUMBER WEEK 36 WEEK 40 WEEK 44 WEEK 48 WEEK 52 WEEK 56 WEEK 60 WEEK 64 WEEK 68 WEEK 72 GROUP: C92571 C92572 C92573 C92574 C92575 C92576 C92577 C92578 C92579 C92580 C92581 C92582 C92583 C92584 C92585 C92586 C92587 C92588 C92589 C92590 C92591 C92592 C92593 C92594 C92595 C92596 C92597 C92598 C92599 C92600 C92601 MALE 2 - 0 . 5 PPM 0 .019 0 .020 0 .016 NOT TAKEN NOT TAKEN 0 .019 0 .018 0 .018 0 .018 0 .018 0 .018 0 .019 0 .019 0 .023 0 .004 0 .019 0 .019 0 .019 0 .018 0 .018 0 .018 0 .018 NOT TAKEN 0 .020 0 .019 0 .019 0 .021 0 .020 0 .020 NOT TAKEN 0 .020 0.019 0. 020 0.014 NOT TAKEN NOT TAKEN 0.018 0.016 0.018 0.017 0.018 0.017 0.018 0.019 0.021 NOT TAKEN 0.018 0.017 0.018 0.018 0.018 0.018 0.017 NOT TAKEN 0.019 0.018 0.018 0. 022 0. 020 0. 020 NOT TAKEN 0.019 0.018 0.018 0.017 NOT TAKEN NOT TAKEN 0.017 0.016 0.017 0.017 0.019 0.017 0.019 0.019 0.024 NOT TAKEN 0.017 0.018 0.019 0.018 0.018 0.017 0.018 NOT TAKEN 0.019 0.018 0.019 0. 022 0. 020 0. 020 NOT TAKEN 0.019 0.018 0.018 0.017 NOT TAKEN NOT TAKEN 0.017 0.015 0.017 0.016 0.018 0.017 0. 020 0.018 0.024 NOT TAKEN 0.016 0.017 0.018 0.017 0.017 0.018 0.016 NOT TAKEN 0.019 0.017 0.019 0. 022 0.019 0.018 NOT TAKEN 0.018 0.017 0.016 0.015 NOT TAKEN NOT TAKEN 0.017 0.016 0.016 0.017 0.017 0.017 0.018 0.018 SPILLED NOT TAKEN 0.015 0.016 0.017 0.017 0.017 0.017 0.016 NOT TAKEN 0.018 0.018 0.018 0. 020 0.018 0.017 NOT TAKEN 0.018 0.017 0.019 0.016 NOT TAKEN NOT TAKEN 0.018 0.016 0.017 0.017 0.018 0.018 0.018 0.018 SPILLED NOT TAKEN 0.016 0.018 0.019 0.018 0.017 0.017 0.018 NOT TAKEN 0.019 0.017 0.018 0. 020 0.018 NOT TAKEN NOT TAKEN 0.018 0.017 0.019 0.015 NOT TAKEN NOT TAKEN 0.017 0.016 0.017 0.018 0.018 0.018 0.019 0.018 NOT TAKEN NOT TAKEN 0.015 0.018 0.019 0.018 0.017 0.016 0.017 NOT TAKEN 0. 020 0.017 0.018 0.019 0.018 NOT TAKEN NOT TAKEN 0.017 0.016 SPILLED 0.015 NOT TAKEN NOT TAKEN 0.017 0.015 0.016 0.017 0.017 0.017 0.018 0.018 NOT TAKEN NOT TAKEN 0.016 0.017 0.019 0.017 0.017 0.017 0.016 NOT TAKEN 0.018 0.018 0.017 0.018 0.017 NOT TAKEN NOT TAKEN 0.017 0.017 0.018 0. 01 0 NOT TAKEN NOT TAKEN 0.017 0.015 0.016 0.016 0.019 0.018 SPILLED 0.017 NOT TAKEN NOT TAKEN 0.005 0.018 0.019 0.018 0.018 0.017 0.017 NOT TAKEN 0. 020 0.016 0.005 0.019 0.017 NOT TAKEN NOT TAKEN 0.018 0.014 0.017 0.016 NOT TAKEN NOT TAKEN 0.016 0.015 0.016 0.014 0.013 0.018 0.017 0.018 NOT TAKEN NOT TAKEN 0. 000 0.017 0.018 0.017 0.017 0.016 0.016 NOT TAKEN 0.018 0.017 NOT TAKEN 0.019 0.017 NOT TAKEN NOT TAKEN 0.017 1472 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 42 ANIMAL NUMBER WEEK 36 WEEK 40 WEEK 44 WEEK 48 WEEK 52 WEEK 56 WEEK 60 WEEK 64 WEEK 68 WEEK 72 GROUP: C92602 C92603 C92604 C92605 C92606 C92607 C92608 C92609 C92610 C92611 C92612 C92613 C92614 C92615 C92616 C92617 C92618 C92619 C92620 C92621 C92622 C92623 C92624 C92625 C92626 C92627 C92628 C92629 C92630 MALE 2 - 0 . 5 PPM 0. 022 NOT TAKEN 0.018 0.017 0.019 0.021 0.016 0. 020 NOT TAKEN 0.018 0.019 NOT TAKEN SPILLED 0.018 NOT TAKEN 0.018 NOT TAKEN 0.018 0. 020 NOT TAKEN 0.019 0.018 0.019 0.018 0.016 NOT TAKEN 0. 022 0.019 0.021 0. 020 NOT TAKEN 0.018 0.017 0.019 0.021 0.019 0. 020 NOT TAKEN 0.018 0.017 NOT TAKEN SPILLED 0.018 NOT TAKEN 0.018 NOT TAKEN 0.019 0. 020 NOT TAKEN 0.017 0.017 0.019 0.017 0.021 NOT TAKEN SPILLED 0.018 0.021 0.021 NOT TAKEN 0.017 0.015 0.018 0.019 0.017 0. 020 NOT TAKEN 0.017 0.017 NOT TAKEN 0.025 0.016 NOT TAKEN 0.017 NOT TAKEN 0. 020 0.019 NOT TAKEN 0.017 0.016 0.017 0.017 0.018 NOT TAKEN 0.023 0.018 0. 020 0.021 NOT TAKEN 0.017 0.015 0.017 0.019 0.019 0.018 NOT TAKEN 0.018 0.016 NOT TAKEN 0.024 0.017 NOT TAKEN 0.016 NOT TAKEN 0. 020 0. 020 NOT TAKEN 0.017 0.017 0.018 0.017 0. 020 NOT TAKEN 0.023 0.017 0. 020 0.021 NOT TAKEN 0.017 0.015 0.017 0.017 0.019 0.018 NOT TAKEN 0.018 0.016 NOT TAKEN SPILLED 0.016 NOT TAKEN 0.016 NOT TAKEN 0.018 0.021 NOT TAKEN 0.016 0.017 0.018 0.015 0. 020 NOT TAKEN 0.012 0.016 0.019 0.021 NOT TAKEN 0.017 0.017 0.019 0.019 0.018 0.019 NOT TAKEN 0.018 0.017 NOT TAKEN SPILLED 0.016 NOT TAKEN 0.019 NOT TAKEN SPILLED 0. 020 NOT TAKEN 0.017 0.017 0.018 0.017 0.017 NOT TAKEN SPILLED 0.017 0. 020 0.023 NOT TAKEN 0.017 0.016 0.018 0.021 0.017 0.018 NOT TAKEN 0.017 0.016 NOT TAKEN 0.026 0.016 NOT TAKEN 0. 020 NOT TAKEN 0.019 0.019 NOT TAKEN 0.017 0.016 0.019 0.017 0.017 NOT TAKEN 0.006 0.017 0.019 0.023 NOT TAKEN 0.016 0.016 0.018 0.019 0.016 0.018 NOT TAKEN 0.018 0.016 NOT TAKEN SPILLED 0.014 NOT TAKEN 0.019 NOT TAKEN 0.019 0. 020 NOT TAKEN 0.017 0.017 NOT TAKEN 0.016 0. 020 NOT TAKEN SPILLED 0.016 0. 020 0.024 NOT TAKEN 0.015 0.015 0.018 0.019 0.017 0.018 NOT TAKEN 0.018 0.015 NOT TAKEN SPILLED 0.016 NOT TAKEN 0. 022 NOT TAKEN 0. 020 0.021 NOT TAKEN 0.017 0.017 NOT TAKEN 0.016 0.021 NOT TAKEN 0.015 0.015 0.019 0.023 NOT TAKEN 0.012 0.016 0.019 0.019 0.009 0.018 NOT TAKEN 0.017 0.013 NOT TAKEN SPILLED 0.015 NOT TAKEN 0.021 NOT TAKEN 0.019 0.019 NOT TAKEN 0.016 0.016 NOT TAKEN 0.016 0.014 NOT TAKEN 0.016 0.012 0. 020 1473 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 43 ANIMAL NUMBER WEEK 36 WEEK 40 WEEK 44 WEEK 48 WEEK 52 WEEK 56 WEEK 60 WEEK 64 WEEK 68 WEEK 72 GROUP: MALE 3 - 2 PPM C92631 C92632 C92633 C92634 C92635 C92636 C92637 C92638 C92639 C92640 C92641 C92642 C92643 C92644 C92645 C92646 C92647 C92648 C92649 C92650 C92651 C92652 C92653 C92654 C92655 C92656 C92657 C92658 C92659 C92660 C92661 0.081 0.071 0.072 0.077 0.077 0.072 0.085 0.070 0.073 NOT TAKEN 0.075 0.066 0.082 0.083 NOT TAKEN NOT TAKEN 0.072 0.078 0.097 NOT TAKEN 0.075 NOT TAKEN 0.073 0.079 0.077 0.069 0.077 0.075 0.076 0.099 0.067 0.078 0.065 0.077 0.075 0.075 0.071 0.084 0.070 0.072 NOT TAKEN 0.084 0.064 0.081 0.081 NOT TAKEN NOT TAKEN 0.074 0.075 0.090 NOT TAKEN 0.074 NOT TAKEN 0.071 0.077 0.082 0.069 0.072 0.073 0.077 SPILLED 0.076 0.077 0.064 0.068 0.077 0.074 0.075 0.079 0.064 0.071 NOT TAKEN 0.076 0.064 0.080 0.077 NOT TAKEN NOT TAKEN 0.073 0.076 0.093 NOT TAKEN 0.074 NOT TAKEN 0.070 0.073 0.076 0.067 0.072 0.073 0.079 0.099 0.074 0.075 0.065 0.070 0.074 0.069 0.078 0.080 0.068 0.069 NOT TAKEN 0.075 0.065 0.086 0.079 NOT TAKEN NOT TAKEN 0.070 0.070 SPILLED NOT TAKEN 0.075 NOT TAKEN 0.064 0.070 0.075 0.063 0.070 0.071 0.076 0.103 0.072 0.071 0.063 0.064 0.071 0.071 0.081 0.078 0.064 0.067 NOT TAKEN 0.084 0.066 0.083 0.077 NOT TAKEN NOT TAKEN 0.067 0.069 0.087 NOT TAKEN 0.077 NOT TAKEN 0.069 0.072 0.075 0.064 0.071 0.067 0.077 SPILLED 0.073 0.075 0.064 0.068 0.078 0.073 0.082 0.076 0.063 0.070 NOT TAKEN 0.073 0.065 SPILLED 0.081 NOT TAKEN NOT TAKEN 0.071 0.075 0. 1 00 NOT TAKEN 0.077 NOT TAKEN 0.062 0.077 SPILLED 0.068 0.075 0.073 0.081 SPILLED 0.074 0.073 0.057 0.069 0.075 0.073 SPILLED 0.081 0.066 0.072 NOT TAKEN 0.047 0.066 0.090 0.081 NOT TAKEN NOT TAKEN 0.068 0.072 0.089 NOT TAKEN 0.078 NOT TAKEN 0.074 0.080 0.073 0.066 0.070 0.075 0.081 SPILLED 0.075 0.073 0.052 0.068 0.071 0.064 SPILLED 0.077 0.063 0.070 NOT TAKEN 0.073 0.060 SPILLED 0.077 NOT TAKEN NOT TAKEN 0.070 0.072 0.078 NOT TAKEN 0.067 NOT TAKEN 0.051 0.086 0.070 0.067 0.072 0.069 0.079 0.098 0.081 0.075 0.066 0.070 0.070 0.073 SPILLED 0.079 0.065 0.066 NOT TAKEN 0.081 0.065 SPILLED 0.077 NOT TAKEN NOT TAKEN 0.071 0.069 0.091 NOT TAKEN 0.066 NOT TAKEN 0.070 0.075 0.078 0.066 0.072 0.070 0.077 0.090 0.078 0.074 0.076 0.065 0.071 0.067 SPILLED 0.073 0.064 0.054 NOT TAKEN 0.067 0.079 0.092 0.078 NOT TAKEN NOT TAKEN 0.072 0.067 0.086 NOT TAKEN 0.061 NOT TAKEN 0.068 0.073 0.075 0.064 0.071 0.067 0.082 0.082 0.077 1474 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 44 ANIMAL NUMBER WEEK 36 WEEK 40 WEEK 44 WEEK 48 WEEK 52 WEEK 56 WEEK 60 WEEK 64 WEEK 68 WEEK 72 GROUP: MALE 3 - 2 PPM C92662 C92663 C92664 C92665 C92666 C92667 C92668 C92669 C92670 C92671 C92672 C92673 C92674 C92675 C92676 C92677 C92678 C92679 C92680 C92681 C92682 C92683 C92684 C92685 C92686 C92687 C92688 C92689 C92690 NOT TAKEN 0.075 0.076 0.071 0.090 0.074 NOT TAKEN 0.076 0.081 0.081 0.069 0.070 0.067 0.085 NOT TAKEN 0.083 NOT TAKEN 0.079 0.070 0.075 0.074 0.073 NOT TAKEN 0.069 0.077 0.060 0.095 0.071 0.071 NOT TAKEN 0.072 0.076 0.070 0.085 0.075 NOT TAKEN 0.076 0.079 0.079 0.073 0.070 0.070 0.085 NOT TAKEN 0.082 NOT TAKEN 0.078 0.073 0.078 0.074 0.073 NOT TAKEN 0.070 0.078 0.080 SPILLED 0.079 0.071 NOT TAKEN 0.071 0.075 0.071 0.076 0.070 NOT TAKEN 0.071 0.077 0.077 0.066 0.072 0.067 0.080 NOT TAKEN 0.077 NOT TAKEN 0.078 0.069 0.075 0.071 0.070 NOT TAKEN 0.066 0.073 0.067 SPILLED 0.074 0.068 NOT TAKEN 0.070 0.074 0.065 0.072 0.068 NOT TAKEN 0.074 0.075 0.076 0.065 0.064 0.063 0.079 NOT TAKEN 0.079 NOT TAKEN 0.081 0.070 0.070 0.070 0.068 NOT TAKEN 0.067 0.074 0.058 SPILLED 0.070 0.072 NOT TAKEN 0.069 0.070 0.064 0.071 0.064 NOT TAKEN 0.068 0.074 0.074 0.065 0.064 0.068 0.077 NOT TAKEN 0.076 NOT TAKEN 0.070 0.067 0.071 0.068 0.067 NOT TAKEN 0.063 0.070 0.066 SPILLED 0.064 0.070 NOT TAKEN 0.070 0.074 0.067 0.071 0.070 NOT TAKEN 0.072 0.081 0.083 0.067 0.072 0.067 0.081 NOT TAKEN 0.080 NOT TAKEN NOT TAKEN 0.074 0.075 0.070 0.062 NOT TAKEN 0.070 0.077 0.064 SPILLED NOT TAKEN 0.072 NOT TAKEN TECH SPILL 0.074 0.069 0.073 0.068 NOT TAKEN 0.071 0.088 0.076 0.068 0.073 0.067 0.076 NOT TAKEN 0.083 NOT TAKEN NOT TAKEN 0.070 0.072 0.071 0.067 NOT TAKEN 0.070 0.078 0.066 0.087 NOT TAKEN 0.069 NOT TAKEN SPILLED 0.073 SPILLED 0.068 0.067 NOT TAKEN 0.071 0.106 0.069 0.068 0.071 0.065 0.077 NOT TAKEN 0.078 NOT TAKEN NOT TAKEN 0.068 0.071 0.067 0.074 NOT TAKEN 0.068 0.077 0.049 0.080 NOT TAKEN 0.069 NOT TAKEN 0.072 0.077 0.069 0.046 0.069 NOT TAKEN 0.068 0.111 0.077 0.066 0.070 0.067 0.075 NOT TAKEN 0.082 NOT TAKEN NOT TAKEN 0.042 0.069 0.068 0.070 NOT TAKEN 0.065 0.072 0.024 0.077 NOT TAKEN 0.066 NOT TAKEN 0.066 0.066 0.068 0.049 0.065 NOT TAKEN 0.072 0. 1 00 0.072 0.070 0.066 0.065 0.074 NOT TAKEN 0.073 NOT TAKEN NOT TAKEN 0.052 0.066 0.066 0.065 NOT TAKEN 0.045 0.072 NOT TAKEN SPILLED NOT TAKEN 0.065 1475 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 45 ANIMAL NUMBER WEEK 36 WEEK 40 WEEK 44 WEEK 48 WEEK 52 WEEK 56 WEEK 60 WEEK 64 WEEK 68 WEEK 72 GROUP: MALE 4 - 5 PPM C92691 C92692 C92693 C92694 C92695 C92696 C92697 C92698 C92699 C92700 C92701 C92702 C92703 C92704 C92705 C92706 C92707 C92708 C92709 C92710 C92711 C92712 C92713 C92714 C92715 C92716 C92717 C92718 C92719 C92720 C92721 0.189 0.172 0.185 0.181 0.199 0.219 0.215 0.173 0.209 0.268 0.181 0.197 0.199 0.173 0.195 0.166 0.186 0.194 0.182 0.195 0.181 0.190 NOT TAKEN NOT TAKEN NOT TAKEN 0.180 0.193 NOT TAKEN NOT TAKEN 0.199 0.195 0.183 0.178 0.171 0.177 0.197 0.206 0.199 0.177 0.198 0.246 0.183 0.198 0.203 0.170 0. 202 0.176 0.190 0.193 0.206 0.198 0.180 0. 202 NOT TAKEN NOT TAKEN NOT TAKEN 0.275 0.194 NOT TAKEN NOT TAKEN 0.203 0.210 0.181 0.142 0.182 0.167 0.182 0.195 0.203 0.173 0.205 0.239 0.181 0.190 0.174 0.163 0.194 0.175 0.190 0.195 0.198 0.197 0.179 0. 200 NOT TAKEN NOT TAKEN NOT TAKEN SPILLED 0.192 NOT TAKEN NOT TAKEN 0.191 0.229 0.181 0.156 0.177 0.163 0.181 0.198 0.196 0.171 0.195 0.219 0.159 0.184 0.181 0.167 0.185 0.169 0.184 0.185 0.196 0.189 0.176 0.184 NOT TAKEN NOT TAKEN NOT TAKEN 0.232 0.185 NOT TAKEN NOT TAKEN 0.184 0.229 0.170 NOT TAKEN 0.171 0.160 0.178 0.194 0.185 0.167 0.201 0.217 0.043 0.185 0.180 0.162 0.180 0.161 0.181 0.180 0.173 0.183 0.164 0.181 NOT TAKEN NOT TAKEN NOT TAKEN SPILLED 0.185 NOT TAKEN NOT TAKEN 0.171 0. 200 0.170 NOT TAKEN 0.173 0.165 0.170 0.197 0.192 0.165 0.207 0.226 NOT TAKEN 0.189 0.186 0.167 0.172 0.166 0.183 0.169 0.179 0.197 0.172 0.201 NOT TAKEN NOT TAKEN NOT TAKEN SPILLED 0.187 NOT TAKEN NOT TAKEN 0.180 0.206 0.189 NOT TAKEN 0.176 0.171 0.196 0.196 0.187 0.160 0.227 SPILLED NOT TAKEN 0.206 0.066 0.168 0.180 0.183 0.203 0.172 0.197 0.191 0.170 0. 200 NOT TAKEN NOT TAKEN NOT TAKEN SPILLED 0.190 NOT TAKEN NOT TAKEN 0.167 0. 222 0.170 NOT TAKEN 0.172 0.161 0.187 0.196 0.121 0.168 0.199 SPILLED NOT TAKEN 0.197 NOT TAKEN 0.155 0.169 0.151 0.180 0.174 0.182 0.183 0.172 0.174 NOT TAKEN NOT TAKEN NOT TAKEN 0.151 0.188 NOT TAKEN NOT TAKEN 0.176 0.213 0.172 NOT TAKEN 0.167 0.166 0.182 0.177 NOT TAKEN 0.164 0.198 0. 220 NOT TAKEN 0.194 NOT TAKEN 0.161 0.172 0.167 0.186 0.189 0.181 0.183 0.170 SPILLED NOT TAKEN NOT TAKEN NOT TAKEN 0.524 0.191 NOT TAKEN NOT TAKEN 0.172 0.208 0.165 NOT TAKEN 0.161 0.161 0.182 0.190 NOT TAKEN 0.156 0.209 0.209 NOT TAKEN 0.195 NOT TAKEN 0.169 0.167 0.173 0.180 0.174 0.180 NOT TAKEN 0.157 0.189 NOT TAKEN NOT TAKEN NOT TAKEN SPILLED 0.198 NOT TAKEN NOT TAKEN 0.174 0. 202 1476 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 46 ANIMAL NUMBER WEEK 36 WEEK 40 WEEK 44 WEEK 48 WEEK 52 WEEK 56 WEEK 60 WEEK 64 WEEK 68 WEEK 72 GROUP: MALE 4 - 5 PPM C92722 C92723 C92724 C92725 C92726 C92727 C92728 C92729 C92730 C92731 C92732 C92733 C92734 C92735 C92736 C92737 C92738 C92739 C92740 C92741 C92742 C92743 C92744 C92745 C92746 C92747 C92748 C92749 C92750 NOT TAKEN 0. 220 0.203 0.193 0.212 0.188 NOT TAKEN 0.190 0.189 NOT TAKEN 0.198 0.238 NOT TAKEN 0.191 0.183 0.180 0. 220 0.214 0.175 0.269 0.167 0.185 NOT TAKEN 0.192 0.173 0.185 0.211 0.190 0.194 NOT TAKEN SPILLED 0.196 0.187 0.209 0.195 NOT TAKEN 0.183 0.181 NOT TAKEN 0.190 0.236 NOT TAKEN 0.192 0.184 0.189 0.201 0.225 0.185 SPILLED 0.168 0.178 NOT TAKEN 0.174 0.185 0. 202 0.224 0. 202 0.184 NOT TAKEN SPILLED 0.207 0.176 0.204 0.182 NOT TAKEN 0.180 0.184 NOT TAKEN 0.183 0.212 NOT TAKEN 0.191 0.180 0.179 0.191 0.208 0.183 SPILLED 0.169 0.177 NOT TAKEN 0.173 0.170 0.188 0.223 0.194 0.177 NOT TAKEN SPILLED 0.189 0.166 0.198 0.189 NOT TAKEN 0.172 0.183 NOT TAKEN 0.175 0.217 NOT TAKEN 0.183 0.177 0.180 0.189 0.208 0.178 SPILLED 0.163 0.167 NOT TAKEN 0.160 0.175 0.183 SPILLED 0.178 0.182 NOT TAKEN SPILLED 0.176 NOT TAKEN 0.197 0.170 NOT TAKEN 0.168 0.170 NOT TAKEN 0.170 0.213 NOT TAKEN 0.177 0.162 0.172 0.180 0.198 0.180 SPILLED 0.156 0.161 NOT TAKEN 0.160 0.168 0.178 SPILLED 0.173 0.167 NOT TAKEN SPILLED 0.169 NOT TAKEN 0.193 0.184 NOT TAKEN 0.181 0.176 NOT TAKEN 0.172 0.218 NOT TAKEN 0.187 0.171 0.170 0.193 0.211 0.179 SPILLED 0.159 0.175 NOT TAKEN 0.163 0.179 0.190 0.213 0.176 0.182 NOT TAKEN 0.227 0.184 NOT TAKEN 0.181 0.184 NOT TAKEN 0.161 0.183 NOT TAKEN 0.188 0.217 NOT TAKEN 0.192 0.183 0.184 0.181 0.203 0.175 SPILLED 0.164 0.161 NOT TAKEN 0.164 0.172 0.185 0.210 0.173 0.173 NOT TAKEN 0.179 0.170 NOT TAKEN 0.181 0.169 NOT TAKEN 0.148 0.182 NOT TAKEN 0.170 0.205 NOT TAKEN 0.179 0.168 0.172 0.182 0.192 0.168 SPILLED 0.160 0.140 NOT TAKEN 0.152 0.168 0.176 SPILLED 0.169 0.170 NOT TAKEN 0.208 0.176 NOT TAKEN 0.186 0.167 NOT TAKEN 0.126 0.171 NOT TAKEN 0.168 0.213 NOT TAKEN 0.171 0.165 0.171 0.187 0.197 0.176 SPILLED 0.163 0.156 NOT TAKEN 0.156 0.181 0.171 SPILLED 0.179 0.169 NOT TAKEN 0.210 0.166 NOT TAKEN 0.193 0.167 NOT TAKEN 0.124 0.176 NOT TAKEN 0.170 0.209 NOT TAKEN 0.178 0.153 0.167 0.188 0.195 0.181 0.170 0.152 0.163 NOT TAKEN 0.149 0.173 0.170 0.141 0.169 0.165 1477 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 47 ANIMAL NUMBER WEEK 36 WEEK 40 WEEK 44 WEEK 48 WEEK 52 WEEK 56 WEEK 60 WEEK 64 WEEK 68 WEEK 72 GROUP: MALE 5 - 2 0 PPM C92751 C92752 C92753 C92754 C92755 C92756 C92757 C92758 C92759 C92760 C92761 C92762 C92763 C92764 C92765 C92766 C92767 C92768 C92769 C92770 C92771 C92772 C92773 C92774 C92775 C92776 C92777 C92778 C92779 C92780 C92781 0.642 NOT TAKEN 0.878 0.827 0.733 0.829 0.880 0.741 NOT TAKEN 0.841 0.967 0.878 0.725 0.901 NOT TAKEN 0.848 0.804 0.819 0.820 0.774 0.740 0.900 0.959 0.746 0.809 0.766 NOT TAKEN 0.769 NOT TAKEN 0.993 0.780 0.708 NOT TAKEN 0.893 0.834 0.752 0.859 SPILLED 0.755 NOT TAKEN 0.845 0.938 0.831 0.690 0.943 NOT TAKEN 0.800 0.744 0.810 0.833 0.785 0.757 0.832 0.928 0.751 0.808 0.782 NOT TAKEN 0.703 NOT TAKEN 1.001 0.779 0.733 NOT TAKEN 0.892 0.815 0.732 0.806 SPILLED 0.705 NOT TAKEN 0.838 0.921 0.809 0.666 0.904 NOT TAKEN 0.798 SPILLED 0.759 0.807 0.740 0.690 0.845 0. 886 0.680 0.581 0.767 NOT TAKEN 0.740 NOT TAKEN 0.970 0.793 0.666 NOT TAKEN 0.622 0.825 0.729 0.810 0.949 0.638 NOT TAKEN 0.812 0.903 0.803 0.651 SPILLED NOT TAKEN 0.726 SPILLED 0.758 0.800 0.728 0.640 0.784 0.892 0.680 0.781 0.737 NOT TAKEN 0.700 NOT TAKEN 0.947 0.748 0.626 NOT TAKEN 0.852 0.795 0.691 0.759 SPILLED 0.549 NOT TAKEN 0.771 0.848 0.772 0.614 0.850 NOT TAKEN 0.710 0.604 0.759 0.745 0.633 0.675 NOT TAKEN 0.852 0.699 0.750 0.715 NOT TAKEN 0.533 NOT TAKEN 0.941 0.717 NOT TAKEN NOT TAKEN 0.822 NOT TAKEN NOT TAKEN 0.749 0.954 0.524 NOT TAKEN 0.766 0.859 0.741 0.640 0.843 NOT TAKEN 0.813 0.636 0.769 0.844 0.733 0.710 NOT TAKEN 0.840 0.706 0.683 0.775 NOT TAKEN NOT TAKEN NOT TAKEN 0.871 0.743 NOT TAKEN NOT TAKEN 0.816 NOT TAKEN NOT TAKEN 0.745 SPILLED 0.586 NOT TAKEN 0.795 0.850 0.731 0.626 0.864 NOT TAKEN 0.887 SPILLED 0.755 0.775 0.717 0.752 NOT TAKEN 0.861 0.698 0.761 0.770 NOT TAKEN NOT TAKEN NOT TAKEN 0.962 0.732 NOT TAKEN NOT TAKEN 0.846 NOT TAKEN NOT TAKEN 0.681 SPILLED NOT TAKEN NOT TAKEN 0.733 0.861 0.745 0.563 0.782 NOT TAKEN 0.788 0.695 0.702 0.714 0.649 0.660 NOT TAKEN 0.861 0.649 0.750 0.630 NOT TAKEN NOT TAKEN NOT TAKEN 0.938 0.774 NOT TAKEN NOT TAKEN 0.826 NOT TAKEN NOT TAKEN 0.661 0.948 NOT TAKEN NOT TAKEN 0.524 0.954 0. 688 0.633 0.666 NOT TAKEN 0.846 SPILLED 0.707 0.749 0.651 0.659 NOT TAKEN 0.915 0.692 0.744 0.754 NOT TAKEN NOT TAKEN NOT TAKEN 1.016 0.806 NOT TAKEN NOT TAKEN 0.825 NOT TAKEN NOT TAKEN 0.719 0.854 NOT TAKEN NOT TAKEN 0.345 0.882 0.716 0.600 0.392 NOT TAKEN 0.789 0.630 0.695 0.740 0.633 0.634 NOT TAKEN 0.854 0.649 0.694 0.708 NOT TAKEN NOT TAKEN NOT TAKEN 0.951 0.116 1478 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 48 ANIMAL NUMBER WEEK 36 WEEK 40 WEEK 44 WEEK 48 WEEK 52 WEEK 56 WEEK 60 WEEK 64 WEEK 68 WEEK 72 GROUP: MALE 5 - 2 0 PPM C92782 C92783 C92784 C92785 C92786 C92787 C92788 C92789 C92790 C92791 C92792 C92793 C92794 C92795 C92796 C92797 C92798 C92799 C92800 C92801 C92802 C92803 C92804 C92805 C92806 C92807 C92808 C92809 C92810 C92811 C92812 0.784 0.738 0.839 0.823 0.815 0.985 0.833 NOT TAKEN 0.769 0.898 0.749 NOT TAKEN 0.797 0.789 0.850 0.895 NOT TAKEN NOT TAKEN 0.797 0.760 0.830 0.821 0.823 0.669 0.662 0.739 0.839 0.806 0.795 NOT TAKEN NOT TAKEN 0.756 0.724 0.811 0.801 0.788 1.064 0.819 NOT TAKEN 0.788 0.850 0.757 NOT TAKEN 0.755 0.804 0.860 0.855 NOT TAKEN NOT TAKEN 0.745 0.719 0.797 0.790 0.813 0.705 0.747 0.695 0.783 0.805 0.787 NOT TAKEN NOT TAKEN 0.726 0.721 0.781 0.803 0.761 SPILLED 0.781 NOT TAKEN 0.741 0.846 0.802 NOT TAKEN 0.762 0.764 0.850 0.864 NOT TAKEN NOT TAKEN 0.819 0.676 0.850 0.836 0.803 0.739 0.800 0.747 0.800 0.781 0.777 NOT TAKEN NOT TAKEN 0.719 0.677 0.784 0.770 0.761 NOT TAKEN 0.768 NOT TAKEN 0.744 0.813 0.756 NOT TAKEN 0.733 0.744 0.840 0.762 NOT TAKEN NOT TAKEN 0.744 0.681 0.773 0.771 0.782 0.694 0.696 0.718 0.780 0.776 0.762 NOT TAKEN NOT TAKEN 0.680 0.662 0.723 0.744 0.710 NOT TAKEN 0.693 NOT TAKEN 0.738 0.736 0.712 NOT TAKEN 0.698 0.617 0.677 0.783 NOT TAKEN NOT TAKEN 0.734 0.580 0.724 0.763 0.717 0.647 0.695 0.637 0.771 0.724 0.708 NOT TAKEN NOT TAKEN 0.702 0.713 0.782 0.789 0.786 NOT TAKEN 0.723 NOT TAKEN NOT TAKEN 0.754 0.776 NOT TAKEN 0.290 NOT TAKEN NOT TAKEN 0.798 NOT TAKEN NOT TAKEN 0.717 NOT TAKEN 0.786 0.768 0.711 0.694 0.739 0.717 0.794 0.737 0.736 NOT TAKEN NOT TAKEN 0.745 0.706 0.733 0.801 0.767 NOT TAKEN 0.797 NOT TAKEN NOT TAKEN 0.791 0.750 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.841 NOT TAKEN NOT TAKEN 0.790 NOT TAKEN 0.774 0.796 0.784 0.765 0.838 0.731 0.818 0.777 0.799 NOT TAKEN NOT TAKEN 0.687 0.646 0.786 0.732 0.744 NOT TAKEN 0.729 NOT TAKEN NOT TAKEN 0.727 0.749 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.792 NOT TAKEN NOT TAKEN 0.775 NOT TAKEN 0.721 0.754 0.739 0.646 0.816 0.661 0.799 0.738 0.734 NOT TAKEN NOT TAKEN 0.679 0.677 0.763 0.774 0.782 NOT TAKEN 0.678 NOT TAKEN NOT TAKEN 0.744 0.765 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.825 NOT TAKEN NOT TAKEN 0.799 NOT TAKEN 0.740 0.757 0.744 0.408 0.784 0.679 0.788 0.722 0.782 NOT TAKEN NOT TAKEN 0.643 0.596 0.806 0.758 0.720 NOT TAKEN 0.698 NOT TAKEN NOT TAKEN 0.704 0.777 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.777 NOT TAKEN NOT TAKEN 0.803 NOT TAKEN 0.701 0.727 0.689 NOT TAKEN 0.743 0.669 0.741 0.729 0.758 NOT TAKEN NOT TAKEN 1479 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 49 ANIMAL NUMBER WEEK 36 WEEK 40 WEEK 44 WEEK 48 WEEK 52 WEEK 56 WEEK 60 WEEK 64 WEEK 68 WEEK 72 GROUP: MALE 5 - 2 0 PPM C92813 C92814 C92815 C92816 C92817 C92818 C92819 C92820 0.847 0.834 SPILLED 0.707 0.742 0.824 0.844 1.133 0.885 0.772 1.137 0.748 0.733 0.804 0.823 1.034 GROUP: MALE 6 - 2 0 PPM C92821 C92822 C92823 C92824 C92825 C92826 C92827 C92828 C92829 C92830 C92831 C92832 C92833 C92834 C92835 C92836 C92837 C92838 C92839 C92840 0.831 0.737 0.834 0.867 0.817 0.862 0.873 0.801 0.814 0.819 0.737 0.793 0.744 0.826 0.778 0.761 0.784 0.835 0.813 0.775 0.803 0.731 0.816 0.750 0.793 0.835 0.819 0.835 0.734 0.913 0.735 0.801 0.752 0.830 0.780 0.822 0.792 0.808 0.771 0.790 0.850 0.815 SPILLED 0.739 0.748 0.801 0.863 0.720 0.734 0.729 0.832 0.810 0.786 0.840 0.844 0.835 0.861 0.915 0.759 0.789 0.730 0.835 0.780 0.780 0.824 0.820 0.735 0.765 0.817 0.788 SPILLED 0.716 0.706 0.815 0.830 1.054 0.759 0.732 0.837 0.787 SPILLED 0.849 0.807 0.845 0.777 0.905 0.744 0.786 0.739 0.815 0.756 0.730 0.802 0.779 SPILLED 0.759 0.763 0.773 SPILLED 0.681 0.500 0.737 0.779 SPILLED 0.733 0.710 0.773 0.777 SPILLED 0.779 0.704 0.787 SPILLED 0.679 0.732 0.741 0.644 0.795 0.680 0.710 0.746 0.711 0.643 0.708 0.793 0.808 SPILLED 0.747 NOT TAKEN 0.815 0.820 NOT TAKEN 0. 000 0. 000 0. 000 0. 000 0. 000 NOT TAKEN 0. 000 0. 000 0. 000 0. 000 0. 000 0. 000 0. 000 0. 000 0. 000 0. 000 0. 000 0. 000 SPILLED 0. 000 0.793 0.794 1.058 0.746 NOT TAKEN 0.794 0.801 NOT TAKEN 0. 000 SPILLED 0. 000 0. 000 0. 000 NOT TAKEN 0. 000 0. 000 0. 000 0. 000 0. 000 0. 000 0. 000 0. 000 0. 000 SPILLED 0. 000 0. 000 0. 000 0. 000 0.805 0.746 TECH SPILL NOT TAKEN NOT TAKEN 0.753 0.768 NOT TAKEN 0.813 0.730 1.027 NOT TAKEN NOT TAKEN 0.797 0.777 NOT TAKEN 0. 000 0. 000 0. 000 0. 000 SPILLED NOT TAKEN 0. 000 0. 000 0. 000 0. 000 0. 000 0. 000 0. 000 0. 000 0. 000 0. 000 0. 000 0. 000 0. 000 0. 000 0. 000 0. 000 SPILLED 0. 000 SPILLED NOT TAKEN 0. 000 0. 000 SPILLED SPILLED 0. 000 0. 000 0. 000 0. 000 0. 000 0. 000 NOT TAKEN NOT TAKEN SPILLED 0. 000 0.769 0.753 0.965 NOT TAKEN NOT TAKEN 0.759 0.748 NOT TAKEN 0. 000 0. 000 0. 000 0. 000 0. 000 NOT TAKEN 0. 000 0. 000 0. 000 0. 000 0. 000 NOT TAKEN 0. 000 0. 000 0. 000 0. 000 NOT TAKEN NOT TAKEN 0. 000 0. 000 1480 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 50 ANIMAL NUMBER WEEK 36 WEEK 40 WEEK 44 WEEK 48 WEEK 52 WEEK 56 WEEK 60 WEEK 64 WEEK 68 WEEK 72 GROUP: MALE 6 - 2 0 PPM C92841 C92842 C92843 C92844 C92845 C92846 C92847 C92848 C92849 C92850 C92851 C92852 C92853 C92854 C92855 C92856 C92857 C92858 C92859 C92860 0.805 0.775 0.783 0.818 0.872 0.820 0.793 0.817 0.919 SPILLED 0.801 0.903 0.788 0.753 0.744 0.835 0.743 0.712 0.792 0.823 0.743 0.752 0.763 0.746 0.902 0.707 0.754 0.820 0.858 SPILLED 0.808 0.889 0.835 0.761 0.822 0.862 0.715 0.706 0.816 0.790 GROUP: FEMALE 2 - 0.5 PPM C92931 C92932 C92933 C92934 C92935 C92936 C92937 C92938 0.021 0.017 0.023 0.026 0. 020 0.024 0.030 0.026 0. 022 SPILLED 0. 022 0.027 0. 022 0. 022 0.028 0. 022 0.791 0.763 0.756 0.848 0.889 0.809 0.733 0.869 0.858 SPILLED 0.793 0.880 0.756 0.785 0.826 0.844 0.658 0.711 0.825 0.831 0.018 NOT TAKEN SPILLED 0.024 0. 020 0. 022 0.026 0.023 0.725 0.736 0.713 0.837 0.850 0.778 0.729 0.820 0.780 SPILLED 0.748 0.852 0.754 0.719 0.708 0.836 0.718 0.733 0.805 0.806 0.018 NOT TAKEN 0. 020 0.023 0.019 0.021 0.025 0. 022 0.693 0.656 0.667 0.816 0.803 0.711 0.698 0.739 0.836 SPILLED 0.737 0.757 0.689 0.691 0.823 0.778 0.685 0.678 0.759 0.807 0.019 NOT TAKEN 0.021 0.024 0.019 0. 022 0.026 0. 022 0. 000 0. 000 SPILLED 0. 000 0. 000 0. 000 0. 000 0. 000 0. 000 SPILLED 0. 000 0. 000 0. 000 0. 000 0. 000 0. 000 SPILLED 0. 000 0. 000 0. 000 0.019 NOT TAKEN 0.021 0.025 0.019 0.024 0.013 0.021 0. 000 0. 000 0. 000 0. 000 0. 000 0. 000 0. 000 0. 000 0. 000 0. 000 0. 000 0. 000 0. 000 0. 000 0. 000 0. 000 0. 000 0. 000 0. 000 0. 000 0. 020 NOT TAKEN 0.023 0. 022 0. 020 0.021 0. 022 0. 020 0. 000 0. 000 0. 000 0. 000 0. 000 0. 000 0. 000 0. 000 0. 000 SPILLED 0. 000 0. 000 0. 000 0. 000 SPILLED 0. 000 SPILLED 0. 000 0. 000 0. 000 0.018 NOT TAKEN 0.019 0.015 0. 020 0.023 0.017 0.021 NOT TAKEN 0. 000 0. 000 0. 000 0. 000 0. 000 0. 000 0. 000 0. 000 0. 000 0. 000 NOT TAKEN 0. 000 0. 000 0. 000 0. 000 SPILLED 0. 000 0. 000 0. 000 0.015 NOT TAKEN 0. 022 0.023 0. 020 0.024 NOT TAKEN 0.019 NOT TAKEN 0. 000 0. 000 0. 000 0. 000 0. 000 0. 000 0. 000 NOT TAKEN 0. 000 0. 000 NOT TAKEN 0. 000 0. 000 NOT TAKEN 0. 000 0. 000 0. 000 NOT TAKEN 0. 000 NOT TAKEN NOT TAKEN 0.018 0. 022 0.019 0.023 NOT TAKEN 0.017 1481 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 51 ANIMAL NUMBER WEEK 36 WEEK 40 WEEK 44 WEEK 48 WEEK 52 WEEK 56 WEEK 60 WEEK 64 WEEK 68 WEEK 72 GROUP: FEMALE 2 - 0 . 5 PPM C92939 C92940 C92941 C92942 C92943 C92944 C92945 C92946 C92947 C92948 C92949 C92950 C92951 C92952 C92953 C92954 C92955 C92956 C92957 C92958 C92959 C92960 C92961 C92962 C92963 C92964 C92965 C92966 C92967 C92968 C92969 0.030 0.027 NOT TAKEN 0.030 0.025 NOT TAKEN NOT TAKEN 0.026 0.025 0.025 NOT TAKEN NOT TAKEN 0.030 0.027 0.024 0.030 0.023 0.023 0.026 0.028 0.035 0.033 0.022 NOT TAKEN 0.029 0.025 0.029 0.027 NOT TAKEN 0.025 NOT TAKEN 0.029 0.029 NOT TAKEN 0.028 0.024 NOT TAKEN NOT TAKEN 0.024 0.023 0.024 NOT TAKEN NOT TAKEN 0.030 SPILLED 0.028 0.030 0.021 0.021 SPILLED 0.026 0.029 0.024 0.020 NOT TAKEN 0.026 SPILLED 0.031 0.026 NOT T0A.0K2E2N NOT TAKEN 0.026 0.027 NOT TAKEN SPILL0E.0D21 NOT TAKEN NOT T0A.0K2E2N 0.025 0.022 NOT TAKEN NOT TAKEN 00..002226 0.023 0.029 0.019 0.023 0.027 0.025 SPILLED 0.027 0.021 NOT TAKEN 0.029 0.025 0.028 0.026 NOT T0A.0K1E2N NOT TAKEN 0.024 0.026 NOT TAKEN 00..002219 NOT TAKEN NOT TAKEN 00..002109 0.020 NOT TAKEN NOT TAKEN 00..002224 0.024 0.028 0.020 0.020 0.023 0.024 0.032 0.024 0.018 NOT TAKEN 0.024 0.023 0.028 0.023 NOT TAKEN 0.019 NOT TAKEN 0.025 0.026 NOT TAKEN NOT T0A.0K2E1N NOT TAKEN NOT TAKEN 00..002128 0.022 NOT TAKEN NOT TAKEN 00..002215 0.021 0.024 0.021 00..002222 0.023 SPILLED 0.024 0.020 NOT TAKEN 00..002226 0.029 0.024 NOT T0A.0K2E1N NOT TAKEN 0.025 0.026 NOT TAKEN NOT TAKEN 0.023 NOT TAKEN NOT TAKEN 00..002127 0.023 NOT TAKEN NOT TAKEN 00..002425 0.021 0.027 0.022 0.022 0.026 0.025 00..002225 0.021 NOT TAKEN 0.023 0.023 0.027 0.025 NOT TAKEN 0.017 NOT TAKEN 00..002214 NOT TAKEN NOT TAKEN 0.019 NOT TAKEN NOT TAKEN 0.018 0.024 0.018 NOT TAKEN NOT TAKEN 00..002214 0.022 0.028 0.020 00..002129 00..001201 0.021 0.019 NOT TAKEN 00..002226 0.025 0.025 NOT TAKEN 0.025 NOT TAKEN 0.028 0.024 NOT TAKEN NOT T0A.0K2E2N NOT TAKEN NOT T0A.0K2E2N 0.022 0.019 NOT TAKEN NOT TAKEN 0.030 TECH 0S.0P2I2LL 0.029 0.020 0.020 00..002203 0.024 0.023 0.019 NOT T0A.0K2E2N SPILL0E.0D21 0.027 NOT T0A.0K2E2N NOT TAKEN 0.026 0.026 NOT TAKEN NOT TAKEN 0.023 NOT TAKEN NOT T0A.0K2E1N 0.023 0.020 NOT TAKEN NOT TAKEN 0.023 NOT T0A.0K2E2N 0.025 0.020 00..002202 00..002202 0.021 0.019 NOT TAKEN 0.024 00..002213 0.016 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN SPILLED NOT TAKEN NOT T0A.0K2E1N NOT TAKEN NOT T0A.0K2E0N 0.021 0.017 NOT TAKEN NOT TAKEN 0.025 NOT T0A.0K2E1N 0.025 0.018 00..002109 00..002202 0.018 0.018 NOT TAKEN 00..002129 0.021 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 1482 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 52 ANIMAL NUMBER WEEK 36 WEEK 40 WEEK 44 WEEK 48 WEEK 52 WEEK 56 WEEK 60 WEEK 64 WEEK 68 WEEK 72 GROUP: FEMALE 2 - 0 . 5 PPM C92970 C92971 C92972 C92973 C92974 C92975 C92976 C92977 C92978 C92979 C92980 C92981 C92982 C92983 C92984 C92985 C92986 C92987 C92988 C92989 C92990 0.021 0.027 0.026 0.028 0.026 0.027 0.026 0.028 NOT TAKEN 0.024 0.025 0.023 NOT TAKEN 0.024 0.023 0.022 0.026 NOT TAKEN 0.026 0.029 0.031 0.022 0.028 0.026 0.027 0.026 0.028 0.026 0.028 NOT TAKEN 0.023 0.023 0.024 NOT TAKEN 0.025 0.024 0.026 0.026 NOT TAKEN 0.027 SPILLED 0.030 GROUP: FEMALE 3 - 2 PPM C92991 C92992 C92993 C92994 C92995 C92996 C92997 0.120 0.129 NOT TAKEN 0.096 NOT TAKEN NOT TAKEN 0.112 0.113 0.113 NOT TAKEN 0.121 NOT TAKEN NOT TAKEN 0.112 0.020 0.025 0.023 0.026 0.023 0.027 0.024 0.023 NOT TAKEN 0.019 0.024 0.022 NOT TAKEN 0.022 0.024 0.022 0.025 NOT TAKEN 0.024 0.029 0.028 0.093 0.101 NOT TAKEN 0.081 NOT TAKEN NOT TAKEN 0.102 0.020 0.024 0.025 0.023 0.024 0.024 0.019 0.025 NOT TAKEN 0.020 0.021 0.021 NOT TAKEN 0.022 0.022 0.020 0.023 NOT TAKEN 0.024 0.026 0.026 0.096 0.098 NOT TAKEN 0.099 NOT TAKEN NOT TAKEN 0.109 0.020 0.025 0.025 0.022 0.023 0.025 0.024 0.024 NOT TAKEN 0.017 0.022 0.021 NOT TAKEN 0.018 0.022 0.017 0.022 NOT TAKEN 0.022 SPILLED 0.017 0.098 0.107 NOT TAKEN 0.100 NOT TAKEN NOT TAKEN 0.107 0.020 0.027 0.025 0.020 0.024 0.025 0.022 0.024 NOT TAKEN 0.022 0.019 0.022 NOT TAKEN 0.023 0.021 0.022 0.024 NOT TAKEN 0.020 0.028 0.024 0.097 0.091 NOT TAKEN 0.095 NOT TAKEN NOT TAKEN 0.104 0.019 0.024 0.020 0.023 0.022 0.024 0.016 0.022 NOT TAKEN 0.021 0.022 0.022 NOT TAKEN 0.021 0.018 0.019 0.022 NOT TAKEN 0.021 0.026 0.021 0.085 0.092 NOT TAKEN 0.071 NOT TAKEN NOT TAKEN 0.098 0.020 0.025 0.023 0.020 0.023 0.026 0.019 0.020 NOT TAKEN 0.020 NOT TAKEN 0.022 NOT TAKEN 0.022 0.023 0.021 0.026 NOT TAKEN 0.021 0.026 0.023 0.095 0.090 NOT TAKEN 0.104 NOT TAKEN NOT TAKEN 0.101 0.019 0.026 0.022 0.023 0.021 0.028 0.024 0.023 NOT TAKEN 0.020 NOT TAKEN 0.015 NOT TAKEN 0.022 0.019 0.025 0.022 NOT TAKEN 0.021 0.025 0.024 0.089 0.094 NOT TAKEN 0.097 NOT TAKEN NOT TAKEN 0.099 0.017 0.021 0.023 0.021 0.022 0.025 0.023 0.023 NOT TAKEN 0.018 NOT TAKEN NOT TAKEN NOT TAKEN 0.022 0.023 0.021 0.022 NOT TAKEN 0.019 0.021 0.022 0.087 0.087 NOT TAKEN 0.096 NOT TAKEN NOT TAKEN 0.092 1483 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 53 ANIMAL NUMBER WEEK 36 WEEK 40 WEEK 44 WEEK 48 WEEK 52 WEEK 56 WEEK 60 WEEK 64 WEEK 68 WEEK 72 GROUP: FEMALE 3 - 2 PPM C92998 C92999 C93000 C93001 C93002 C93003 C93004 C93005 C93006 C93007 C93008 C93009 C93010 C93011 C93012 C93013 C93014 C93015 C93016 C93017 C93018 C93019 C93020 C93021 C93022 C93023 C93024 C93025 C93026 C93027 C93028 0.095 0.099 NOT TAKEN 0.112 0.110 0.098 0.087 0.122 NOT TAKEN 0.101 NOT TAKEN SPILLED 0.118 0.090 0.117 0.097 0.102 0.108 0.132 0.104 NOT TAKEN 0.087 0.114 0.094 0.085 NOT TAKEN 0.106 0.105 0.121 0.104 0.113 0.035 0.100 NOT TAKEN 0.121 0.108 0.108 0.111 0.096 NOT TAKEN 0.100 NOT TAKEN 0.106 0.114 0.088 0.107 0.106 0.098 0.110 0.123 0.100 NOT TAKEN 0.084 0.117 0.102 0.099 NOT TAKEN 0.108 0.108 0.118 0.106 0.117 TECH SPILL 0.095 NOT TAKEN 0.097 0.104 0.087 0.094 0.091 NOT TAKEN 0.089 NOT TAKEN SPILLED 0.078 0.089 0.103 0.092 0.091 0.106 0.114 0.103 NOT TAKEN SPILLED 0.077 0.088 0.083 NOT TAKEN 0.106 0.087 0.105 0.098 0.107 NOT TAKEN 0.087 NOT TAKEN 0.109 0.107 0.078 0.092 0.097 NOT TAKEN 0.090 NOT TAKEN 0.092 0.084 0.084 0.108 0.089 0.081 0.098 0.124 0.106 NOT TAKEN NOT TAKEN 0.097 0.090 0.072 NOT TAKEN 0.104 0.099 0.101 0.105 0.107 NOT TAKEN 0.088 NOT TAKEN 0.096 0.108 0.095 0.081 0.101 NOT TAKEN 0.092 NOT TAKEN 0.091 0.089 0.081 0.101 0.091 0.076 0.114 0.107 0.099 NOT TAKEN NOT TAKEN 0.081 0.082 0.066 NOT TAKEN 0.100 0.091 0.104 0.090 0.109 NOT TAKEN 0.088 NOT TAKEN 0.094 0.101 0.089 0.086 0.104 NOT TAKEN 0.090 NOT TAKEN 0.092 0.093 0.082 0.104 0.085 0.076 0.098 0.122 0.104 NOT TAKEN NOT TAKEN 0.091 0.042 0.083 NOT TAKEN 0.092 0.087 0.099 0.101 0.093 NOT TAKEN 0.092 NOT TAKEN 0.098 0.109 0.088 0.083 0.094 NOT TAKEN 0.085 NOT TAKEN 0.083 0.083 0.080 0.098 0.088 0.081 0.104 0.117 0.103 NOT TAKEN NOT TAKEN 0.064 0.041 0.079 NOT TAKEN 0.093 0.089 0.101 0.087 0.090 NOT TAKEN 0.090 NOT TAKEN 0.091 0.099 0.088 0.079 0.084 NOT TAKEN 0.088 NOT TAKEN 0.083 0.083 0.077 0.101 0.084 0.074 0.090 0.072 SPILLED NOT TAKEN NOT TAKEN 0.026 NOT TAKEN 0.078 NOT TAKEN 0.089 0.068 0.100 0.085 0.103 NOT TAKEN 0.100 NOT TAKEN 0.094 0.102 0.094 0.058 0.097 NOT TAKEN 0.080 NOT TAKEN 0.081 0.080 0.087 0.089 0.084 0.048 0.072 0.125 0.109 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.078 NOT TAKEN 0.092 0.086 0.074 0.090 0.092 NOT TAKEN 0.083 NOT TAKEN 0.087 0.091 0.078 0.070 0.090 NOT TAKEN 0.073 NOT TAKEN 0.079 0.083 0.079 SPILLED 0.078 0.044 0.108 SPILLED 0.101 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.051 NOT TAKEN 0.082 SPILLED 0.103 0.085 0.083 1484 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 54 ANIMAL NUMBER WEEK 36 WEEK 40 WEEK 44 WEEK 48 WEEK 52 WEEK 56 WEEK 60 WEEK 64 WEEK 68 WEEK 72 GROUP: FEMALE 3 - 2 PPM C93029 C93030 C93031 C93032 C93033 C93034 C93035 C93036 C93037 C93038 C93039 C93040 C93041 C93042 C93043 C93044 C93045 C93046 C93047 C93048 C93049 C93050 SPILLED 0.098 NOT TAKEN 0.101 0.120 0.104 NOT TAKEN 0.092 0.098 0.116 0.098 0.128 0.104 0.098 0.109 0.121 NOT TAKEN 0.110 0.087 CNTMD 0.103 0.127 SPILLED 0.091 NOT TAKEN 0.105 0.106 0.110 NOT TAKEN 0.102 0.108 0.121 0.103 0.125 0.109 0.095 0.103 0.108 NOT TAKEN 0.117 0.097 0.125 0.096 0.115 GROUP: FEMALE 4 - 5 PPM C93051 C93052 C93053 C93054 C93055 C93056 NOT TAKEN 0.237 0.250 0.309 0.221 0.286 NOT TAKEN 0.254 0.232 0.314 0.258 0.277 SPILLED 0.088 NOT TAKEN 0.093 0.126 0.098 NOT TAKEN 0.101 0.084 0.105 0.088 0.145 0.102 0.091 0.112 0.103 NOT TAKEN 0.109 0.091 0.105 0.089 0.108 NOT TAKEN 0.229 0.213 0.275 0.232 0.266 0.129 0.082 NOT TAKEN 0.103 0.125 0.103 NOT TAKEN 0.097 0.098 0.104 0.083 0.123 0.105 0.087 0.115 0.100 NOT TAKEN 0.110 0.078 0.106 0.101 0.107 NOT TAKEN 0.225 0.221 0.281 0.229 0.260 SPILLED 0.082 NOT TAKEN 0.094 0.067 0.103 NOT TAKEN 0.089 0.094 0.109 0.098 0.113 0.100 0.078 0.123 0.102 NOT TAKEN 0.087 0.075 0.098 0.091 0.107 NOT TAKEN 0.226 0.225 0.263 0.221 0.246 0.126 0.081 NOT TAKEN 0.092 0.131 0.108 NOT TAKEN 0.079 0.081 0.104 0.083 0.107 0.104 0.093 SPILLED 0.094 NOT TAKEN 0.104 0.084 0.118 0.089 0.102 NOT TAKEN 0.203 0.229 0.248 0.197 0.251 0.130 0.077 NOT TAKEN 0.089 SPILLED 0.103 NOT TAKEN 0.087 0.080 0.088 0.083 0.127 0.097 0.088 0.108 0.042 NOT TAKEN 0.100 0.074 0.108 0.093 0.098 NOT TAKEN 0.181 0.121 0.215 0.201 0.233 SPILLED 0.077 NOT TAKEN 0.085 0.070 0.105 NOT TAKEN 0.080 0.053 0.078 0.079 0.126 0.096 0.087 0.105 0.048 NOT TAKEN 0.093 0.080 0.104 0.048 0.094 NOT TAKEN 0.123 0.153 0.203 0.201 0.238 SPILLED 0.084 NOT TAKEN 0.087 NOT TAKEN 0.109 NOT TAKEN 0.077 NOT TAKEN 0.079 0.083 SPILLED 0.095 0.078 SPILLED NOT TAKEN NOT TAKEN 0.087 0.076 0.104 0.042 0.094 NOT TAKEN 0.117 NOT TAKEN 0.259 0.192 0.248 NOT TAKEN 0.072 NOT TAKEN 0.075 NOT TAKEN 0.095 NOT TAKEN 0.076 NOT TAKEN NOT TAKEN 0.080 0.122 0.090 0.082 SPILLED NOT TAKEN NOT TAKEN 0.081 0.076 0.095 NOT TAKEN 0.081 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.187 0.229 1485 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 55 ANIMAL NUMBER WEEK 36 WEEK 40 WEEK 44 WEEK 48 WEEK 52 WEEK 56 WEEK 60 WEEK 64 WEEK 68 WEEK 72 GROUP: FEMALE 4 - 5 PPM C93057 C93058 C93059 C93060 C93061 C93062 C93063 C93064 C93065 C93066 C93067 C93068 C93069 C93070 C93071 C93072 C93073 C93074 C93075 C93076 C93077 C93078 C93079 C93080 C93081 C93082 C93083 C93084 C93085 C93086 C93087 0.263 NOT TAKEN 0.269 0.239 0.269 0.291 0.249 NOT TAKEN 0.306 SPILLED NOT TAKEN 0.270 0.240 NOT TAKEN NOT TAKEN 0.253 0.278 0.216 NOT TAKEN 0.269 0.254 0.289 0.244 SPILLED 0.236 0.237 0.252 NOT TAKEN NOT TAKEN 0.267 0.246 0.268 NOT TAKEN 0.259 0.296 0.254 0.276 0.242 NOT TAKEN 0.291 SPILLED NOT TAKEN 0.273 0.255 NOT TAKEN NOT TAKEN 0.234 0.296 0.217 NOT TAKEN 0.256 0.240 0.266 0.250 SPILLED 0.255 0.240 0.273 NOT TAKEN NOT TAKEN 0.257 0.273 0.223 NOT TAKEN 0.235 0.257 0.260 0.259 0.223 NOT TAKEN 0.282 SPILLED NOT TAKEN 0.251 0.249 NOT TAKEN NOT TAKEN 0.226 0.269 0.126 NOT TAKEN 0.255 0.248 0.268 0.267 SPILLED 0.223 0.219 0.262 NOT TAKEN NOT TAKEN 0.255 0.264 0.250 NOT TAKEN 0.247 0.267 0.249 0.259 0.227 NOT TAKEN 0.295 0.274 NOT TAKEN 0.262 0.236 NOT TAKEN NOT TAKEN 0.242 SPILLED 0.275 NOT TAKEN 0.221 0.231 0.257 0.249 0.257 0.251 0.231 0.244 NOT TAKEN NOT TAKEN 0.231 0.232 0.226 NOT TAKEN 0.217 0.298 0.249 0.261 0.206 NOT TAKEN 0.282 SPILLED NOT TAKEN 0.243 0.245 NOT TAKEN NOT TAKEN 0.232 0.328 0.219 NOT TAKEN 0.210 0.193 0.247 0.239 SPILLED 0.230 0.196 0.235 NOT TAKEN NOT TAKEN 0.224 0.231 0.227 NOT TAKEN 0.225 0.262 0.266 0.251 0.216 NOT TAKEN 0.259 0.289 NOT TAKEN 0.260 0.220 NOT TAKEN NOT TAKEN 0.212 0.286 0.204 NOT TAKEN 0.234 0.226 0.340 0.230 SPILLED 0.265 0.210 0.226 NOT TAKEN NOT TAKEN 0.216 0.236 0.223 NOT TAKEN 0.234 0.251 0.263 0.248 0.206 NOT TAKEN 0.261 0.222 NOT TAKEN 0.228 0.216 NOT TAKEN NOT TAKEN 0.202 0.257 0.188 NOT TAKEN 0.212 0.186 0.225 0.187 0.223 0.225 0.222 0.212 NOT TAKEN NOT TAKEN 0.222 0.214 0.225 NOT TAKEN 0.223 0.235 SPILLED 0.242 0.201 NOT TAKEN 0.268 SPILLED NOT TAKEN 0.233 0.214 NOT TAKEN NOT TAKEN 0.224 0.238 0.187 NOT TAKEN 0.180 0.273 0.236 0.219 SPILLED 0.201 0.226 0.217 NOT TAKEN NOT TAKEN 0.231 0.222 0.244 NOT TAKEN 0.213 0.259 0.272 0.233 0.223 NOT TAKEN 0.257 SPILLED NOT TAKEN 0.238 0.224 NOT TAKEN NOT TAKEN 0.236 0.266 0.182 NOT TAKEN 0.250 0.255 0.240 0.222 0.256 0.220 0.217 0.232 NOT TAKEN NOT TAKEN 0.209 0.250 0.225 NOT TAKEN 0.202 0.180 0.221 0.225 0.189 NOT TAKEN 0.212 SPILLED NOT TAKEN 0.242 0.190 NOT TAKEN NOT TAKEN 0.226 0.128 0.168 NOT TAKEN 0.211 0.238 0.235 0.192 SPILLED 0.194 0.215 0.225 NOT TAKEN NOT TAKEN 0.220 0.207 1486 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 56 ANIMAL NUMBER WEEK 36 WEEK 40 WEEK 44 WEEK 48 WEEK 52 WEEK 56 WEEK 60 WEEK 64 WEEK 68 WEEK 72 GROUP: FEMALE 4 - 5 PPM C93088 C93089 C93090 C93091 C93092 C93093 C93094 C93095 C93096 C93097 C93098 C93099 C93100 C93101 C93102 C93103 C93104 C93105 C93106 C93107 C93108 C93109 C93110 0.282 0.260 0.253 0.251 0.298 0.274 0.258 0.254 NOT TAKEN 0.295 0.261 0.250 0.256 0.241 0.296 0.316 0.248 0.266 0.257 0.226 0.242 NOT TAKEN 0.241 0.291 0.289 0.261 0.258 0.314 0.260 0.275 0.246 NOT TAKEN 0.276 0.228 0.239 0.313 0.227 0.284 0.304 0.215 0.269 0.247 0.255 0.243 NOT TAKEN 0.252 GROUP: FEMALE 5 - 20 PPM C93111 C93112 C93113 C93114 C93115 NOT TAKEN 1.040 1.173 NOT TAKEN 1.257 NOT TAKEN 1.061 1.266 NOT TAKEN 1.215 0.251 0.213 0.267 0.233 0.272 0.232 0.242 0.245 NOT TAKEN 0.256 0.228 0.221 0.273 0.262 0.281 0.257 0.227 0.257 0.256 0.219 0.214 NOT TAKEN 0.246 NOT TAKEN SPILLED 1.108 NOT TAKEN 1.116 0.262 0.273 0.247 0.244 0.276 0.254 0.233 0.235 NOT TAKEN 0.249 0.204 0.222 0.268 0.255 0.281 0.257 0.227 0.260 0.241 SPILLED 0.221 NOT TAKEN 0.216 NOT TAKEN 1.137 1.043 NOT TAKEN 1.080 0.290 0.253 0.226 0.235 0.271 0.213 0.231 0.223 NOT TAKEN SPILLED 0.202 0.223 0.241 0.207 0.269 0.234 0.210 0.246 0.203 0.228 NOT TAKEN NOT TAKEN 0.248 NOT TAKEN 0.855 1.127 NOT TAKEN 1.067 0.293 0.268 0.237 0.228 0.269 0.225 0.235 0.222 NOT TAKEN 0.230 0.224 0.215 0.251 0.204 0.254 0.231 0.221 0.252 0.204 0.210 NOT TAKEN NOT TAKEN 0.210 NOT TAKEN NOT TAKEN 1.032 NOT TAKEN 1.034 0.285 0.236 0.242 0.225 0.237 0.210 0.208 0.216 NOT TAKEN 0.234 0.245 0.201 0.247 0.205 0.241 0.234 0.211 0.238 0.228 0.224 NOT TAKEN NOT TAKEN 0.250 NOT TAKEN NOT TAKEN 0.959 NOT TAKEN 0.968 0.293 0.233 0.249 0.221 SPILLED 0.206 0.228 0.209 NOT TAKEN 0.227 0.210 0.199 0.256 0.192 0.289 0.230 0.202 0.253 0.210 0.232 NOT TAKEN NOT TAKEN 0.201 NOT TAKEN NOT TAKEN 1.006 NOT TAKEN 0.997 0.208 0.232 0.244 0.218 0.266 0.192 0.210 0.228 NOT TAKEN 0.267 0.211 0.192 0.238 0.191 0.261 0.229 0.167 0.247 0.216 0.222 NOT TAKEN NOT TAKEN 0.188 NOT TAKEN NOT TAKEN 0.969 NOT TAKEN 0.978 0.166 0.246 0.222 0.240 0.125 0.223 0.200 0.207 NOT TAKEN 0.207 0.209 0.183 0.230 0.217 0.225 0.211 0.231 SPILLED 0.229 0.209 NOT TAKEN NOT TAKEN 0.223 NOT TAKEN NOT TAKEN 0.980 NOT TAKEN 0.863 1487 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 57 ANIMAL NUMBER WEEK 36 WEEK 40 WEEK 44 WEEK 48 WEEK 52 WEEK 56 WEEK 60 WEEK 64 WEEK 68 WEEK 72 GROUP: C93116 C93117 C93118 C93119 C93120 C93121 C93122 C93123 C93124 C93125 C93126 C93127 C93128 C93129 C93130 C93131 C93132 C93133 C93134 C93135 C93136 C93137 C93138 C93139 C93140 C93141 C93142 C93143 C93144 C93145 C93146 FEMALE 5 - 2 0 PPM 1.220 1.229 1.058 1.143 1.207 1.050 1.034 NOT TAKEN 1.030 1.216 1.103 NOT TAKEN 1.004 1.208 1.049 1.097 1.049 1.070 1.066 1.257 1.242 1.246 0.957 1.326 1.123 1.118 NOT TAKEN NOT TAKEN NOT TAKEN SPILLED 0.984 1.242 1.198 1.163 1.199 1.255 1.117 1.122 NOT TAKEN 1.074 1.266 1.169 NOT TAKEN 1.134 1.314 1.140 1.019 0.983 1.414 0.998 1.321 1.298 1.180 1.018 1.172 1.095 1.123 NOT TAKEN NOT TAKEN NOT TAKEN SPILLED 1.207 1.212 1.104 1.129 1.123 1.141 SPILLED 1.049 NOT TAKEN 1.215 1.257 1.065 NOT TAKEN 1.105 1.255 0.973 1.028 0.931 SPILLED 0.990 1.140 1.201 1.192 0.889 1.072 0.922 1.023 NOT TAKEN NOT TAKEN NOT TAKEN SPILLED 1.055 1.203 SPILLED 1.081 1.116 1.112 1.067 1.126 NOT TAKEN 1.119 1.163 1.059 NOT TAKEN 1.043 1.251 0.999 0.993 0.972 1.183 1.007 1.195 1.232 1.171 0.944 1.123 0.924 0.955 NOT TAKEN NOT TAKEN NOT TAKEN 1.354 1.400 1.116 1.398 1.006 1.173 0.991 1.111 1.040 NOT TAKEN 1.151 1.189 1.024 NOT TAKEN 1.013 1.294 1.031 0.959 0.811 0.896 1.011 1.180 1.256 1.030 1.028 1.070 0.987 1.020 NOT TAKEN NOT TAKEN NOT TAKEN SPILLED 1.115 NOT TAKEN NOT TAKEN 0.980 1.092 NOT TAKEN 1.081 0.967 NOT TAKEN 1.080 1.163 0.599 NOT TAKEN 0.957 1.156 NOT TAKEN 0.919 0.900 1.171 0.897 1.301 NOT TAKEN 0.914 NOT TAKEN 1.005 0.952 0.963 NOT TAKEN NOT TAKEN NOT TAKEN 1.240 1.126 NOT TAKEN NOT TAKEN 1.014 1.028 NOT TAKEN 1.090 0.989 NOT TAKEN 1.087 1.210 NOT TAKEN NOT TAKEN 0.959 1.091 NOT TAKEN 0.835 0.909 0.837 0.650 SPILLED NOT TAKEN 1.066 NOT TAKEN 1.034 0.977 0.894 NOT TAKEN NOT TAKEN NOT TAKEN SPILLED 1.194 NOT TAKEN NOT TAKEN 1.093 SPILLED NOT TAKEN 3.483 0.961 NOT TAKEN 1.101 1.179 NOT TAKEN NOT TAKEN 1.108 SPILLED NOT TAKEN 0.856 0.905 0.987 0.658 1.314 NOT TAKEN 0.927 NOT TAKEN 0.983 0.981 1.105 NOT TAKEN NOT TAKEN NOT TAKEN 1.133 1.133 NOT TAKEN NOT TAKEN 1.287 1.245 NOT TAKEN 1.118 1.006 NOT TAKEN 1.026 1.179 NOT TAKEN NOT TAKEN 1.043 1.396 NOT TAKEN 0.940 0.947 1.047 NOT TAKEN 1.401 NOT TAKEN 1.003 NOT TAKEN 0.931 0.965 0.960 NOT TAKEN NOT TAKEN NOT TAKEN 1.059 1.253 NOT TAKEN NOT TAKEN 1.120 1.268 NOT TAKEN 0.857 1.038 NOT TAKEN 1.022 1.084 NOT TAKEN NOT TAKEN 0.985 1.163 NOT TAKEN 0.856 0.806 0.935 NOT TAKEN SPILLED NOT TAKEN 0.974 NOT TAKEN 0.974 0.887 0.936 NOT TAKEN NOT TAKEN NOT TAKEN 1.270 1.173 1488 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 58 ANIMAL NUMBER WEEK 36 WEEK 40 WEEK 44 WEEK 48 WEEK 52 WEEK 56 WEEK 60 WEEK 64 WEEK 68 WEEK 72 GROUP: FEMALE 5 - 2 0 PPM C93147 C93148 C93149 C93150 C93151 C93152 C93153 C93154 C93155 C93156 C93157 C93158 C93159 C93160 C93161 C93162 C93163 C93164 C93165 C93166 C93167 C93168 C93169 C93170 C93171 C93172 C93173 C93174 C93175 C93176 C93177 1.043 1.164 1.026 1.310 1.162 1.151 1.224 1.199 NOT TAKEN 1.127 1.154 1.130 NOT TAKEN 1.203 1.152 1.053 1.118 1.055 1.126 0.867 0.907 1.105 NOT TAKEN 0.858 1.108 1.046 1.041 SPILLED 1.091 1.051 1.167 1.113 1.189 1.212 1.252 1.437 1.194 1.287 1.166 NOT TAKEN 1.078 1.215 1.526 NOT TAKEN 1.245 0.996 1.121 1.133 1.147 1.061 1.182 NOT TAKEN 1.170 NOT TAKEN SPILLED 1.715 1.075 1.137 SPILLED 1.107 0.995 1.172 1.052 1.182 1.042 1.240 1.042 1.164 1.262 1.014 NOT TAKEN 1.024 1.101 1.137 NOT TAKEN 1.192 1.040 0.809 0.950 1.210 0.975 0.772 NOT TAKEN 1.049 NOT TAKEN SPILLED 1.054 1.005 1.013 SPILLED 1.065 0.888 1.109 0.980 1.171 1.014 1.202 1.036 1.241 1.262 1.093 NOT TAKEN 1.022 1.011 1.100 NOT TAKEN SPILLED 1.086 0.999 1.016 1.181 0.996 0.807 NOT TAKEN 1.091 NOT TAKEN 1.624 1.014 1.012 0.999 1.420 1.060 0.874 1.116 1.073 1.115 0.984 1.211 1.077 1.358 1.243 0.967 NOT TAKEN 0.971 1.029 1.140 NOT TAKEN SPILLED 1.025 1.008 0.924 1.192 1.004 1.055 NOT TAKEN 1.177 NOT TAKEN 1.200 0.927 0.972 0.947 1.376 1.072 0.899 1.120 NOT TAKEN 1.149 0.975 1.120 1.060 1.020 1.089 NOT TAKEN NOT TAKEN 1.003 0.982 1.017 NOT TAKEN 1.190 0.964 1.074 NOT TAKEN 1.041 0.988 0.812 NOT TAKEN 1.114 NOT TAKEN 1.265 NOT TAKEN 1.096 1.058 SPILLED 1.019 0.924 1.255 NOT TAKEN 1.156 0.988 1.161 1.109 1.043 1.136 NOT TAKEN NOT TAKEN 0.972 0.959 0.975 NOT TAKEN 1.112 0.868 1.119 NOT TAKEN 1.112 0.965 0.893 NOT TAKEN 1.051 NOT TAKEN SPILLED NOT TAKEN 0.892 0.964 0.958 1.028 0.831 1.113 NOT TAKEN 1.068 0.984 1.184 1.111 1.071 1.153 NOT TAKEN NOT TAKEN 1.061 0.960 0.953 NOT TAKEN SPILLED 0.986 1.030 NOT TAKEN 1.064 1.004 0.975 NOT TAKEN 1.118 NOT TAKEN SPILLED NOT TAKEN 1.075 SPILLED SPILLED 1.051 0.879 1.146 NOT TAKEN 1.022 1.060 1.201 1.147 NOT TAKEN 1.248 NOT TAKEN NOT TAKEN 0.948 0.934 0.955 NOT TAKEN SPILLED 0.765 1.095 NOT TAKEN 1.133 1.004 0.852 NOT TAKEN 1.070 NOT TAKEN SPILLED NOT TAKEN 0.948 0.964 1.078 0.989 0.899 1.275 NOT TAKEN 0.982 0.903 1.175 1.124 NOT TAKEN 1.201 NOT TAKEN NOT TAKEN 0.958 0.862 0.939 NOT TAKEN 0.081 0.981 SPILLED NOT TAKEN 1.015 0.941 0.857 NOT TAKEN 1.005 NOT TAKEN SPILLED NOT TAKEN 0.425 0.941 0.918 1.028 0.753 1.073 1489 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104 -WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 59 ANIMAL NUMBER WEEK 36 WEEK 40 WEEK 44 WEEK 48 WEEK 52 WEEK 56 WEEK 60 WEEK 64 WEEK 68 WEEK 72 GROUP: C93178 C93179 C93180 GROUP: C93181 C93182 C93183 C93184 C93185 C93186 C93187 C93188 C93189 C93190 C93191 C93192 C93193 C93194 C93195 C93196 C93197 C93198 C93199 C93200 C93201 C93202 C93203 C93204 C93205 FEMALE 5 - 20 PPM 1.033 1.156 1.082 1.024 1.207 1.106 FEMALE 6 - 20 PPM 1.134 1.097 1.031 1.231 1.137 1.209 1.036 1.055 1.049 1.156 1.288 1.238 1.016 1.082 1.065 1.111 1.140 0.983 1.118 1.174 1.047 1.044 1.119 1.100 1.070 1.171 1.122 0.859 1.214 1.112 1.222 1.054 1.091 1.255 1.193 1.210 1.296 1.134 1.074 1.124 1.135 1.275 1.017 1.097 1.204 1.263 0.973 1.065 1.129 1.044 0.907 1.050 1.011 1.071 1.027 0.784 1.190 1.149 1.362 0.964 1.017 1.571 1.125 1.095 1.155 0.972 1.014 1.045 1.118 1.093 1.001 1.056 1.102 1.061 0.986 0.998 1.099 0.975 0.995 1.073 1.130 1.094 1.052 0.957 1.164 1.087 1.199 0.935 0.958 1.320 1.155 0.984 1.180 0.951 0.968 1.009 1.050 1.265 0.955 1.026 1.114 1.410 1.013 0.993 1.141 1.071 0.918 1.015 1.043 0.909 1.135 0.911 0.886 1.011 0.666 0 .928 1 .035 NOT TAKEN 0.885 1.029 NOT TAKEN 0.882 1.012 NOT TAKEN 1.131 1.076 0.933 1.132 1.046 1.202 0.963 0.952 1.262 1.108 0.964 1.114 0.895 1.053 0.998 1.098 1.254 0.960 0.999 1.133 1.052 0.930 1.127 1.076 1.037 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 SPILLED 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 SPILLED 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0 .000 0 .000 0 .000 0 .000 0 .000 0 .000 0 .000 0 .000 0 .000 0 .000 0 .000 0 .000 0 .000 0 .000 0 .000 0 .000 SPILLED 0 .000 0 .000 0 .000 SPILLED 0 .000 0 .000 0 .000 0 .000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 SPILLED 0.000 0.000 0.000 0.000 0.000 0.000 0.000 NOT TAKEN 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 SPILLED 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 NOT TAKEN 0.000 0.000 0.000 NOT TAKEN 0.000 0.000 0.000 0.000 0.000 0.000 1490 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104 -WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS Covance 6329-183 3M T-6295 PAGE: 60 ANIMAL NUMBER WEEK 36 WEEK 40 WEEK 44 WEEK 48 WEEK 52 WEEK 56 WEEK 60 WEEK 64 WEEK 68 WEEK 72 GROUP: C93206 C93207 C93208 C93209 C93210 C93211 C93212 C93213 C93214 C93215 C93216 C93217 C93218 C93219 C93220 FEMALE 6 - 20 PPM 1.156 1.211 1.225 SPILLED 0.987 1.169 1.096 1.061 1.146 1.000 1.095 1.141 1.073 0.938 0.983 1.206 SPILLED 1.054 SPILLED 1.043 1.192 1.185 1.076 1.158 1.039 1.051 1.107 1.151 1.004 1.042 1.186 NOT TAKEN 1.073 SPILLED 1.006 1.135 1.072 0.854 1.098 0.983 1.024 1.014 1.039 0.997 0.976 1.167 NOT TAKEN 1.057 SPILLED 0.954 1.163 0.998 0.874 1.010 0.906 0.992 0.998 1.020 0.924 0.936 1.103 NOT TAKEN 0.910 SPILLED 0.994 1.112 0.978 1.240 1.026 0.975 1.013 1.000 1.076 0.918 0.928 0 .000 NOT TAKEN 0 .000 SPILLED SPILLED SPILLED 0 .000 0 .000 0 .000 0 .000 0 .000 0 .000 0 .000 0 .000 0 .000 0 .000 NOT TAKEN 0 .000 SPILLED 0 .000 0 .000 0 .000 0 .000 0 .000 0 .000 0 .000 0 .000 0 .000 0 .000 0 .000 0 .000 NOT TAKEN 0 .000 0 .000 0 .000 0 .000 0 .000 0 .000 0 .000 0 .000 0 .000 0 .000 0 .000 0 .000 0 .000 0 .000 NOT TAKEN 0 .000 0 .000 0 .000 0 .000 0 .000 0 .000 0 .000 0 .000 0 .000 0 .000 0 .000 0 .000 0 .000 0 .000 NOT TAKEN 0 .000 0 .000 SPILLED 0 .000 0 .000 0 .000 0 .000 0 .000 0 .000 0 .000 0 .000 0 .000 0 .000 1491 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 76 WEEK 80 WEEK 84 WEEK 88 WEEK 92 WEEK 96 WEEK 100 WEEK 104 GROUP: MALE 2 - 0.5 PPM C92571 C92572 C92573 C92574 C92575 C92576 C92577 C92578 C92579 C92580 C92581 C92582 C92583 C92584 C92585 C92586 C92587 C92588 C92589 C92590 C92591 C92592 C92593 C92594 C92595 C92596 C92597 C92598 C92599 C92600 C92601 0.017 0.016 0.015 NOT TAKEN NOT TAKEN 0.016 0.014 0.014 0.013 0.005 0.018 0.017 0.017 NOT TAKEN NOT TAKEN NOT TAKEN 0.017 0.019 0.019 0.017 0.017 0.015 NOT TAKEN 0.018 0.016 NOT TAKEN 0.017 0.017 NOT TAKEN NOT TAKEN 0.016 0.018 0.017 0.009 NOT TAKEN NOT TAKEN 0.014 0.012 0.012 0.010 NOT TAKEN 0.015 0.014 0.015 NOT TAKEN NOT TAKEN NOT TAKEN 0.016 0.018 0.016 0.016 0.016 0.015 NOT TAKEN 0.017 0.015 NOT TAKEN 0.018 0.017 NOT TAKEN NOT TAKEN 0.018 0.017 0.017 NOT TAKEN NOT TAKEN NOT TAKEN 0.016 0.015 0.006 0.013 NOT TAKEN 0.017 0.017 0.017 NOT TAKEN NOT TAKEN NOT TAKEN 0.011 0.021 0.017 0.017 0.016 0.016 NOT TAKEN 0.018 NOT TAKEN NOT TAKEN 0.018 0.017 NOT TAKEN NOT TAKEN 0.017 0.017 0.018 NOT TAKEN NOT TAKEN NOT TAKEN 0.016 0.015 NOT TAKEN NOT TAKEN NOT TAKEN 0.016 0.017 0.018 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.021 0.018 0.017 0.016 NOT TAKEN NOT TAKEN 0.018 NOT TAKEN NOT TAKEN 0.014 0.017 NOT TAKEN NOT TAKEN 0.016 0.016 0.018 NOT TAKEN NOT TAKEN NOT TAKEN 0.017 0.015 NOT TAKEN NOT TAKEN NOT TAKEN 0.014 0.016 0.017 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.023 0.016 0.017 0.015 NOT TAKEN NOT TAKEN 0.017 NOT TAKEN NOT TAKEN 0.017 0.017 NOT TAKEN NOT TAKEN 0.017 0.009 0.019 NOT TAKEN NOT TAKEN NOT TAKEN 0.011 0.014 NOT TAKEN NOT TAKEN NOT TAKEN 0.011 0.014 0.018 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.020 0.013 0.017 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.017 0.017 NOT TAKEN NOT TAKEN 0.017 SPILLED 0.008 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.014 NOT TAKEN NOT TAKEN NOT TAKEN 0.023 SPILLED 0.016 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.011 NOT TAKEN 0.018 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.017 0.016 NOT TAKEN NOT TAKEN 0.017 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.013 NOT TAKEN NOT TAKEN NOT TAKEN 0.000 NOT TAKEN 0.015 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.018 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN SPILLED 0.015 NOT TAKEN NOT TAKEN 0.016 Covance 6329-183 3M T-6295 PAGE: 61 1492 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 76 WEEK 80 WEEK 84 WEEK 88 WEEK 92 WEEK 96 WEEK 100 WEEK 104 GROUP: C92602 C92603 C92604 C92605 C92606 C92607 C92608 C92609 C92610 C92611 C92612 C92613 C92614 C92615 C92616 C92617 C92618 C92619 C92620 C92621 C92622 C92623 C92624 C92625 C92626 C92627 C92628 C92629 C92630 MALE 2 - 0.5 PPM 0.022 NOT TAKEN 0.009 0.015 0.018 0.018 0.003 0.017 NOT TAKEN 0.018 0.004 NOT TAKEN SPILLED 0.014 NOT TAKEN 0.013 NOT TAKEN 0.018 0.020 NOT TAKEN 0.016 0.016 NOT TAKEN 0.015 0.017 NOT TAKEN NOT TAKEN 0.011 0.020 0.026 NOT TAKEN 0.012 0.016 NOT TAKEN 0.018 NOT TAKEN 0.018 NOT TAKEN 0.016 NOT TAKEN NOT TAKEN SPILLED 0.014 NOT TAKEN 0.008 NOT TAKEN 0.018 0.020 NOT TAKEN 0.014 0.015 NOT TAKEN 0.011 0.018 NOT TAKEN NOT TAKEN NOT TAKEN 0.017 0.023 NOT TAKEN 0.012 0.016 NOT TAKEN 0.010 NOT TAKEN 0.018 NOT TAKEN 0.015 NOT TAKEN NOT TAKEN SPILLED 0.013 NOT TAKEN NOT TAKEN NOT TAKEN 0.018 0.024 NOT TAKEN NOT TAKEN 0.016 NOT TAKEN NOT TAKEN 0.019 NOT TAKEN NOT TAKEN NOT TAKEN 0.008 0.022 NOT TAKEN 0.013 0.016 NOT TAKEN 0.011 NOT TAKEN 0.017 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN SPILLED 0.005 NOT TAKEN NOT TAKEN NOT TAKEN 0.018 0.022 NOT TAKEN NOT TAKEN 0.016 NOT TAKEN NOT TAKEN SPILLED NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.020 NOT TAKEN NOT TAKEN 0.014 NOT TAKEN NOT TAKEN NOT TAKEN 0.017 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.019 0.015 NOT TAKEN NOT TAKEN 0.016 NOT TAKEN NOT TAKEN SPILLED NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.019 NOT TAKEN NOT TAKEN 0.011 NOT TAKEN NOT TAKEN NOT TAKEN 0.018 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.018 NOT TAKEN NOT TAKEN NOT TAKEN 0.016 NOT TAKEN NOT TAKEN 0.018 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.018 NOT TAKEN NOT TAKEN 0.014 NOT TAKEN NOT TAKEN NOT TAKEN 0.017 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.019 NOT TAKEN NOT TAKEN NOT TAKEN 0.016 NOT TAKEN NOT TAKEN SPILLED NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.019 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.018 NOT TAKEN NOT TAKEN NOT TAKEN 0.017 NOT TAKEN NOT TAKEN 0.014 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN Covance 6329-183 3M T-6295 PAGE: 62 1493 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 76 WEEK 80 WEEK 84 WEEK 88 WEEK 92 WEEK 96 WEEK 100 WEEK 104 GROUP: MALE 3 - 2 PPM C92631 C92632 C92633 C92634 C92635 C92636 C92637 C92638 C92639 C92640 C92641 C92642 C92643 C92644 C92645 C92646 C92647 C92648 C92649 C92650 C92651 C92652 C92653 C92654 C92655 C92656 C92657 C92658 C92659 C92660 C92661 0.071 0.066 0.069 0.068 0.013 SPILLED 0.075 0.065 0.047 NOT TAKEN 0.064 0.060 0.084 0.076 NOT TAKEN NOT TAKEN 0.060 0.062 0.079 NOT TAKEN 0.045 NOT TAKEN 0.065 0.041 0.072 0.066 0.070 0.063 0.070 0.091 0.084 0.073 0.063 0.067 0.069 NOT TAKEN SPILLED 0.072 0.065 0.089 NOT TAKEN 0.063 0.061 0.082 0.077 NOT TAKEN NOT TAKEN NOT TAKEN 0.063 SPILLED NOT TAKEN 0.030 NOT TAKEN 0.065 NOT TAKEN 0.072 0.064 0.065 0.069 0.072 0.084 0.096 0.073 0.065 0.066 0.067 NOT TAKEN 0.063 0.073 0.048 0.020 NOT TAKEN 0.066 0.066 0.085 0.075 NOT TAKEN NOT TAKEN NOT TAKEN SPILLED 0.077 NOT TAKEN NOT TAKEN NOT TAKEN 0.066 NOT TAKEN 0.071 0.070 0.071 0.069 SPILLED 0.089 0.010 0.069 0.063 0.063 NOT TAKEN NOT TAKEN SPILLED 0.075 0.056 NOT TAKEN NOT TAKEN 0.066 0.063 0.072 0.079 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.082 NOT TAKEN NOT TAKEN NOT TAKEN 0.060 NOT TAKEN 0.059 0.069 0.071 0.064 0.057 0.079 0.076 0.073 SPILLED 0.066 NOT TAKEN NOT TAKEN SPILLED 0.077 NOT TAKEN NOT TAKEN NOT TAKEN 0.072 0.066 0.074 0.078 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.088 NOT TAKEN NOT TAKEN NOT TAKEN 0.063 NOT TAKEN 0.049 0.040 0.075 0.061 0.063 SPILLED NOT TAKEN 0.073 0.069 0.072 NOT TAKEN NOT TAKEN SPILLED 0.075 NOT TAKEN NOT TAKEN NOT TAKEN 0.080 0.065 0.082 0.072 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.082 NOT TAKEN NOT TAKEN NOT TAKEN 0.061 NOT TAKEN NOT TAKEN 0.020 0.072 NOT TAKEN SPILLED 0.087 NOT TAKEN 0.071 0.070 0.070 NOT TAKEN NOT TAKEN NOT TAKEN 0.090 NOT TAKEN NOT TAKEN NOT TAKEN SPILLED 0.067 0.072 0.071 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.079 NOT TAKEN NOT TAKEN NOT TAKEN 0.062 NOT TAKEN NOT TAKEN NOT TAKEN 0.070 NOT TAKEN SPILLED 0.085 NOT TAKEN 0.070 NOT TAKEN 0.082 NOT TAKEN NOT TAKEN NOT TAKEN 0.115 NOT TAKEN NOT TAKEN NOT TAKEN 0.035 0.067 0.073 0.060 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.079 NOT TAKEN NOT TAKEN NOT TAKEN 0.036 NOT TAKEN NOT TAKEN NOT TAKEN 0.067 NOT TAKEN SPILLED 0.082 NOT TAKEN Covance 6329-183 3M T-6295 PAGE: 63 1494 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 76 WEEK 80 WEEK 84 WEEK 88 WEEK 92 WEEK 96 WEEK 100 WEEK 104 GROUP: MALE 3 - 2 PPM C92662 C92663 C92664 C92665 C92666 C92667 C92668 C92669 C92670 C92671 C92672 C92673 C92674 C92675 C92676 C92677 C92678 C92679 C92680 C92681 C92682 C92683 C92684 C92685 C92686 C92687 C92688 C92689 C92690 NOT TAKEN 0.071 0.032 0.065 NOT T0A.0K0E2N NOT TAKEN 0.065 NOT TAKEN 0.075 0.064 0.072 0.066 0.071 NOT TAKEN 0.076 NOT TAKEN NOT TAKEN 0.074 0.071 0.067 0.063 NOT TAKEN 0.051 0.074 NOT TAKEN SPILLED NOT TAKEN 0.065 NOT TAKEN 0.067 NOT TAKEN 0.065 NOT TAKEN NOT TAKEN NOT TAKEN 0.069 NOT TAKEN 0.070 0.062 0.073 0.064 0.070 NOT TAKEN 0.068 NOT TAKEN NOT TAKEN 0.057 0.069 0.073 0.066 NOT TAKEN NOT TAKEN 0.054 NOT TAKEN SPILLED NOT TAKEN 0.065 NOT TAKEN 0.064 NOT TAKEN SPILLED NOT TAKEN NOT TAKEN NOT TAKEN 0.071 NOT TAKEN 0.084 0.070 0.069 0.034 0.071 NOT TAKEN 0.074 NOT TAKEN NOT TAKEN NOT TAKEN 0.071 0.068 0.064 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN SPILLED NOT TAKEN 0.057 NOT TAKEN 0.070 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.067 NOT TAKEN 0.075 0.064 0.064 0.060 0.067 NOT TAKEN 0.075 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.076 0.065 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN SPILLED NOT TAKEN 0.068 NOT TAKEN 0.061 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.070 NOT TAKEN 0.068 0.052 0.058 0.060 0.061 NOT TAKEN 0.075 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.071 0.061 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.048 NOT TAKEN 0.063 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.070 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.064 0.053 NOT TAKEN 0.077 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.063 0.061 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.061 NOT TAKEN 0.062 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.070 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.062 0.040 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.070 0.059 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.065 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.059 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.060 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.069 SPILLED NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN SPILLED Covance 6329-183 3M T-6295 PAGE: 64 1495 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 76 WEEK 80 WEEK 84 WEEK 88 WEEK 92 WEEK 96 WEEK 100 WEEK 104 GROUP: MALE 4 - 5 PPM C92691 C92692 C92693 C92694 C92695 C92696 C92697 C92698 C92699 C92700 C92701 C92702 C92703 C92704 C92705 C92706 C92707 C92708 C92709 C92710 C92711 C92712 C92713 C92714 C92715 C92716 C92717 C92718 C92719 C92720 C92721 0.160 NOT TAKEN SPILLED 0.157 0.172 0.169 NOT TAKEN 0.151 0.210 0.190 NOT TAKEN 0.184 NOT TAKEN 0.159 0.167 0.162 0.174 0.177 0.175 NOT TAKEN 0.152 0.170 NOT TAKEN NOT TAKEN NOT TAKEN SPILLED 0.196 NOT TAKEN NOT TAKEN 0.171 0.217 0.166 NOT TAKEN 0.168 0.161 0.180 0.203 NOT TAKEN 0.152 0.198 0.209 NOT TAKEN 0.184 NOT TAKEN 0.160 0.158 SPILLED 0.176 0.157 0.162 NOT TAKEN 0.165 0.154 NOT TAKEN NOT TAKEN NOT TAKEN SPILLED 0.186 NOT TAKEN NOT TAKEN 0.123 NOT TAKEN 0.169 NOT TAKEN 0.173 0.164 0.177 0.201 NOT TAKEN 0.163 0.189 0.223 NOT TAKEN 0.190 NOT TAKEN 0.171 0.164 0.163 0.179 0.197 0.137 NOT TAKEN 0.174 0.150 NOT TAKEN NOT TAKEN NOT TAKEN SPILLED 0.193 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.176 NOT TAKEN 0.174 0.157 0.185 0.198 NOT TAKEN 0.159 0.181 0.201 NOT TAKEN 0.176 NOT TAKEN 0.165 0.163 0.192 0.032 0. 200 0.162 NOT TAKEN 0.094 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN SPILLED 0.199 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.159 NOT TAKEN 0.170 0.158 0.168 0.184 NOT TAKEN 0.152 0.179 0.231 NOT TAKEN 0.184 NOT TAKEN 0.157 0.164 SPILLED NOT TAKEN 0.180 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN SPILLED 0.188 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.173 NOT TAKEN 0.140 0.157 0.176 0.191 NOT TAKEN 0.141 0.176 0.210 NOT TAKEN 0.191 NOT TAKEN 0.164 0.169 0.139 NOT TAKEN 0.180 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.232 0.173 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.172 NOT TAKEN 0.140 0.151 0.176 0.187 NOT TAKEN 0.096 0.191 0.176 NOT TAKEN 0.127 NOT TAKEN 0.154 0.183 NOT TAKEN NOT TAKEN 0.179 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN SPILLED 0.176 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.153 NOT TAKEN 0.148 0.152 0.185 0.188 NOT TAKEN 0.013 0.185 0.149 NOT TAKEN 0.117 NOT TAKEN 0.148 0.175 NOT TAKEN NOT TAKEN 0.197 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN SPILLED 0.193 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN Covance 6329-183 3M T-6295 PAGE: 65 1496 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 76 WEEK 80 WEEK 84 WEEK 88 WEEK 92 WEEK 96 WEEK 100 WEEK 104 GROUP: MALE 4 - 5 PPM C92722 C92723 C92724 C92725 C92726 C92727 C92728 C92729 C92730 C92731 C92732 C92733 C92734 C92735 C92736 C92737 C92738 C92739 C92740 C92741 C92742 C92743 C92744 C92745 C92746 C92747 C92748 C92749 C92750 NOT TAKEN SPILLED 0.160 NOT TAKEN 0.189 0.164 NOT TAKEN 0.118 0.173 NOT TAKEN 0.163 0.191 NOT TAKEN 0.178 0.162 0.166 0.201 0.192 0.169 SPILLED SPILLED 0.163 NOT TAKEN 0.154 0.188 0.173 SPILLED 0.171 0.173 NOT TAKEN NOT TAKEN 0.149 NOT TAKEN 0.188 0.160 NOT TAKEN NOT TAKEN 0.168 NOT TAKEN 0.161 0.199 NOT TAKEN 0.174 0.156 0.164 0.215 0.193 0.187 SPILLED 0.150 0.154 NOT TAKEN 0.144 NOT TAKEN 0.159 0.217 0.176 0.163 NOT TAKEN NOT TAKEN 0.165 NOT TAKEN 0.187 0.161 NOT TAKEN NOT TAKEN 0.170 NOT TAKEN NOT TAKEN 0.108 NOT TAKEN 0.177 0.162 0.155 0.221 0.196 0.180 0.145 0.146 NOT TAKEN NOT TAKEN 0.084 NOT TAKEN 0.167 0.205 0.180 0.169 NOT TAKEN NOT TAKEN 0.172 NOT TAKEN 0.191 0.115 NOT TAKEN NOT TAKEN 0.162 NOT TAKEN NOT TAKEN 0.036 NOT TAKEN 0.173 0.153 0.067 0.285 0.184 0.177 0.037 0.151 NOT TAKEN NOT TAKEN 0.049 NOT TAKEN SPILLED 0.139 0.168 0.168 NOT TAKEN NOT TAKEN 0.168 NOT TAKEN 0.183 NOT TAKEN NOT TAKEN NOT TAKEN 0.165 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.163 0.149 NOT TAKEN NOT TAKEN 0.187 0.155 NOT TAKEN 0.159 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN SPILLED 0.188 0.167 0.159 NOT TAKEN NOT TAKEN 0.168 NOT TAKEN SPILLED NOT TAKEN NOT TAKEN NOT TAKEN 0.138 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.168 0.155 NOT TAKEN NOT TAKEN 0.193 0.168 NOT TAKEN 0.157 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN SPILLED 0.176 0.173 0.172 NOT TAKEN NOT TAKEN 0.154 NOT TAKEN 0.219 NOT TAKEN NOT TAKEN NOT TAKEN 0.115 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.169 0.175 NOT TAKEN NOT TAKEN 0.186 0.172 NOT TAKEN 0.156 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN SPILLED 0.182 0.171 0.159 NOT TAKEN NOT TAKEN 0.164 NOT TAKEN 0.203 NOT TAKEN NOT TAKEN NOT TAKEN 0.106 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.171 0.160 NOT TAKEN NOT TAKEN 0.158 0.170 NOT TAKEN 0.143 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN CNTMD 0.137 NOT TAKEN 0.098 Covance 6329-183 3M T-6295 PAGE: 66 1497 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 76 WEEK 80 WEEK 84 WEEK 88 WEEK 92 WEEK 96 WEEK 100 WEEK 104 GROUP: MALE 5 - 2 0 PPM C92751 C92752 C92753 C92754 C92755 C92756 C92757 C92758 C92759 C92760 C92761 C92762 C92763 C92764 C92765 C92766 C92767 C92768 C92769 C92770 C92771 C92772 C92773 C92774 C92775 C92776 C92777 C92778 C92779 C92780 C92781 NOT TAKEN NOT TAKEN 0.823 NOT TAKEN NOT TAKEN 0.711 0.867 NOT TAKEN NOT TAKEN NOT TAKEN 0.840 0.670 SPILLED NOT TAKEN NOT TAKEN 0.864 0.449 0.712 0.720 0.661 0.627 NOT TAKEN 0.921 0.661 0.670 0.715 NOT TAKEN NOT TAKEN NOT TAKEN SPILLED 0.310 NOT TAKEN NOT TAKEN 0.748 NOT TAKEN NOT TAKEN 0.671 0.767 NOT TAKEN NOT TAKEN NOT TAKEN 0.863 0.624 0.547 NOT TAKEN NOT TAKEN 0.726 0.609 0.696 0.753 SPILLED 0.095 NOT TAKEN 0.942 0.609 0.685 0.681 NOT TAKEN NOT TAKEN NOT TAKEN 0.946 NOT TAKEN NOT TAKEN NOT TAKEN 0.851 NOT TAKEN NOT TAKEN 0.313 0.766 NOT TAKEN NOT TAKEN NOT TAKEN 0.816 0.660 0.575 NOT TAKEN NOT TAKEN 0.770 0.617 0.692 0.738 SPILLED NOT TAKEN NOT TAKEN 0.852 0.665 0.724 0.755 NOT TAKEN NOT TAKEN NOT TAKEN SPILLED NOT TAKEN NOT TAKEN NOT TAKEN 0.812 NOT TAKEN NOT TAKEN NOT TAKEN 0.760 NOT TAKEN NOT TAKEN NOT TAKEN 0.965 0.690 0.557 NOT TAKEN NOT TAKEN 0.720 0.697 0.683 0.602 0.703 NOT TAKEN NOT TAKEN 0.904 0.695 0.667 0.763 NOT TAKEN NOT TAKEN NOT TAKEN 0.952 NOT TAKEN NOT TAKEN NOT TAKEN 0.765 NOT TAKEN NOT TAKEN NOT TAKEN 0.666 NOT TAKEN NOT TAKEN NOT TAKEN 0.881 0.680 0.203 NOT TAKEN NOT TAKEN 0.750 0.541 0.508 0.771 0.653 NOT TAKEN NOT TAKEN 0.950 0.649 0.648 0.769 NOT TAKEN NOT TAKEN NOT TAKEN 0.986 NOT TAKEN NOT TAKEN NOT TAKEN 0.795 NOT TAKEN NOT TAKEN NOT TAKEN 0.584 NOT TAKEN NOT TAKEN NOT TAKEN 1.029 0.667 NOT TAKEN NOT TAKEN NOT TAKEN 0.778 0.128 NOT TAKEN 0.723 0.678 NOT TAKEN NOT TAKEN 0.939 0.645 0.613 0.728 NOT TAKEN NOT TAKEN NOT TAKEN 0.802 NOT TAKEN NOT TAKEN NOT TAKEN 0.656 NOT TAKEN NOT TAKEN NOT TAKEN 0.643 NOT TAKEN NOT TAKEN NOT TAKEN 1.251 0.654 NOT TAKEN NOT TAKEN NOT TAKEN 0.727 NOT TAKEN NOT TAKEN 0.560 0.395 NOT TAKEN NOT TAKEN 0.993 0.694 0.573 0.690 NOT TAKEN NOT TAKEN NOT TAKEN 0.578 NOT TAKEN NOT TAKEN NOT TAKEN 0.582 NOT TAKEN NOT TAKEN NOT TAKEN 0.461 NOT TAKEN NOT TAKEN NOT TAKEN SPILLED 0.676 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.879 0.621 0.174 0.735 NOT TAKEN NOT TAKEN NOT TAKEN 0.357 NOT TAKEN Covance 6329-183 3M T-6295 PAGE: 67 1498 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 76 WEEK 80 WEEK 84 WEEK 88 WEEK 92 WEEK 96 WEEK 100 WEEK 104 GROUP: MALE 5 - 2 0 PPM C92782 C92783 C92784 C92785 C92786 C92787 C92788 C92789 C92790 C92791 C92792 C92793 C92794 C92795 C92796 C92797 C92798 C92799 C92800 C92801 C92802 C92803 C92804 C92805 C92806 C92807 C92808 C92809 C92810 C92811 C92812 0.667 0.393 0.506 0.693 0.730 NOT TAKEN 0.745 NOT TAKEN NOT TAKEN 0.737 0.716 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.753 NOT TAKEN NOT TAKEN 0.771 NOT TAKEN 0.687 0.710 0.685 NOT TAKEN 0.723 0.656 0.800 0.703 0.737 NOT TAKEN NOT TAKEN 0.677 NOT TAKEN NOT TAKEN 0.730 0.710 NOT TAKEN 0.690 NOT TAKEN NOT TAKEN 0.709 0.696 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.701 NOT TAKEN NOT TAKEN 0.721 NOT TAKEN 0.678 0. 688 0.675 NOT TAKEN 0.734 0.483 0.776 0.711 0.728 NOT TAKEN NOT TAKEN 0.618 NOT TAKEN NOT TAKEN 0.722 0.706 NOT TAKEN 0.745 NOT TAKEN NOT TAKEN 0.748 0.753 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.819 NOT TAKEN NOT TAKEN 0.750 NOT TAKEN 0.685 0.722 0.726 NOT TAKEN 0.664 0.407 0.791 0.744 0.765 NOT TAKEN NOT TAKEN 0.631 NOT TAKEN NOT TAKEN 0.663 0.704 NOT TAKEN 0.722 NOT TAKEN NOT TAKEN 0.690 0. 688 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.640 NOT TAKEN NOT TAKEN 0.679 NOT TAKEN 0.621 0.712 0.675 NOT TAKEN 0.740 NOT TAKEN 0.805 0.725 0.550 NOT TAKEN NOT TAKEN 0.610 NOT TAKEN NOT TAKEN 0.678 0.711 NOT TAKEN 0.682 NOT TAKEN NOT TAKEN 0.698 0.667 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.115 NOT TAKEN NOT TAKEN 0.654 NOT TAKEN 0.637 0.732 0.641 NOT TAKEN 0.680 NOT TAKEN 0.796 0.757 0.381 NOT TAKEN NOT TAKEN 0.719 NOT TAKEN NOT TAKEN 0.625 0.692 NOT TAKEN 0.682 NOT TAKEN NOT TAKEN 0.711 0.691 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.710 NOT TAKEN 0.653 0.691 0.650 NOT TAKEN 0.617 NOT TAKEN 0.844 0.773 0.196 NOT TAKEN NOT TAKEN 0.658 NOT TAKEN NOT TAKEN NOT TAKEN 0.712 NOT TAKEN 0.649 NOT TAKEN NOT TAKEN 0.683 0.695 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.652 NOT TAKEN 0.637 0.710 0.699 NOT TAKEN NOT TAKEN NOT TAKEN 0.783 0.658 NOT TAKEN NOT TAKEN NOT TAKEN 0.601 NOT TAKEN NOT TAKEN NOT TAKEN 0.695 NOT TAKEN 0.691 NOT TAKEN NOT TAKEN 0.699 0.573 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.680 NOT TAKEN 0.574 0.731 0.695 NOT TAKEN NOT TAKEN NOT TAKEN 0.776 0.564 NOT TAKEN NOT TAKEN NOT TAKEN Covance 6329-183 3M T-6295 PAGE: 68 1499 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 76 WEEK 80 WEEK 84 WEEK 88 WEEK 92 WEEK 96 WEEK 100 WEEK 104 GROUP: MALE 5 - 2 0 PPM C92813 C92814 C92815 C92816 C92817 C92818 C92819 C92820 0.785 0.735 SPILLED NOT TAKEN NOT TAKEN 0.704 0.730 NOT TAKEN 0.678 0.774 0.937 NOT TAKEN NOT TAKEN 0.709 0.744 NOT TAKEN GROUP: MALE 6 - 2 0 PPM C92821 C92822 C92823 C92824 C92825 C92826 C92827 C92828 C92829 C92830 C92831 C92832 C92833 C92834 C92835 C92836 C92837 C92838 C92839 C92840 0. 000 0. 000 0. 000 0. 000 0. 000 NOT TAKEN 0. 000 0. 000 0. 000 0. 000 0. 000 NOT TAKEN 0. 000 0. 000 NOT TAKEN 0. 000 NOT TAKEN NOT TAKEN 0. 000 0. 000 0. 000 0. 000 0. 000 0. 000 0. 000 NOT TAKEN 0. 000 0. 000 0. 000 0. 000 0. 000 NOT TAKEN 0. 000 0. 000 NOT TAKEN 0. 000 NOT TAKEN NOT TAKEN SPILLED 0. 000 0.753 0.716 SPILLED NOT TAKEN NOT TAKEN 0.737 0.791 NOT TAKEN 0. 000 NOT TAKEN 0. 000 0. 000 0. 000 NOT TAKEN 0. 000 NOT TAKEN SPILLED 0. 000 0. 000 NOT TAKEN 0. 000 0. 000 NOT TAKEN 0. 000 NOT TAKEN NOT TAKEN 0. 000 0. 000 0.673 0.741 0.941 NOT TAKEN NOT TAKEN 0.778 0.788 NOT TAKEN 0. 000 NOT TAKEN 0. 000 0. 000 0. 000 NOT TAKEN 0. 000 NOT TAKEN SPILLED SPILLED NOT TAKEN NOT TAKEN 0. 000 0. 000 NOT TAKEN 0. 000 NOT TAKEN NOT TAKEN 0. 000 0. 000 0.775 0.766 SPILLED NOT TAKEN NOT TAKEN 0.741 0.834 NOT TAKEN 0. 000 NOT TAKEN 0. 000 0. 000 0. 000 NOT TAKEN 0. 000 NOT TAKEN SPILLED 0. 000 NOT TAKEN NOT TAKEN SPILLED 0. 000 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0. 000 0.720 0.730 SPILLED NOT TAKEN NOT TAKEN 0.734 SPILLED NOT TAKEN 0. 000 NOT TAKEN 0. 000 NOT TAKEN NOT TAKEN NOT TAKEN 0. 000 NOT TAKEN SPILLED 0. 000 NOT TAKEN NOT TAKEN SPILLED 0. 000 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0. 000 NOT TAKEN 0. 688 0.944 NOT TAKEN NOT TAKEN 0.714 0.811 NOT TAKEN 0. 000 NOT TAKEN 0. 000 NOT TAKEN NOT TAKEN NOT TAKEN 0. 000 NOT TAKEN SPILLED NOT TAKEN NOT TAKEN NOT TAKEN SPILLED 0. 000 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0. 000 NOT TAKEN 0.729 0.925 NOT TAKEN NOT TAKEN 0.720 NOT TAKEN NOT TAKEN 0. 000 NOT TAKEN 0. 000 NOT TAKEN NOT TAKEN NOT TAKEN 0. 000 NOT TAKEN 0. 000 NOT TAKEN NOT TAKEN NOT TAKEN 0. 000 0. 000 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN Covance 6329-183 3M T-6295 PAGE: 69 1500 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 76 WEEK 80 WEEK 84 WEEK 88 WEEK 92 WEEK 96 WEEK 100 WEEK 104 GROUP: MALE 6 - 2 0 PPM C92841 C92842 C92843 C92844 C92845 C92846 C92847 C92848 C92849 C92850 C92851 C92852 C92853 C92854 C92855 C92856 C92857 C92858 C92859 C92860 NOT TAKEN 0. 000 0. 000 0. 000 0. 000 0. 000 0. 000 0. 000 NOT TAKEN 0. 000 0. 000 NOT TAKEN 0. 000 0. 000 NOT TAKEN 0. 000 0. 000 0. 000 NOT TAKEN 0. 000 NOT TAKEN 0. 000 NOT TAKEN 0. 000 0. 000 0. 000 0. 000 0. 000 NOT TAKEN 0. 000 0. 000 NOT TAKEN 0. 000 0. 000 NOT TAKEN 0. 000 0. 000 0. 000 NOT TAKEN 0. 000 GROUP: FEMALE 2 - 0.5 PPM C92931 C92932 C92933 C92934 C92935 C92936 C92937 C92938 NOT TAKEN NOT TAKEN 0.018 0. 020 0.014 0.024 NOT TAKEN 0.019 NOT TAKEN NOT TAKEN 0.017 0.018 0.008 0. 022 NOT TAKEN 0.019 NOT TAKEN 0. 000 NOT TAKEN NOT TAKEN 0. 000 0. 000 0. 000 0. 000 NOT TAKEN 0. 000 0. 000 NOT TAKEN 0. 000 0. 000 NOT TAKEN 0. 000 NOT TAKEN 0. 000 NOT TAKEN 0. 000 NOT TAKEN NOT TAKEN 0. 020 0.013 0.011 0.024 NOT TAKEN 0.019 NOT TAKEN 0. 000 NOT TAKEN NOT TAKEN 0. 000 0. 000 0. 000 0. 000 NOT TAKEN 0. 000 0. 000 NOT TAKEN 0. 000 0. 000 NOT TAKEN 0. 000 NOT TAKEN 0. 000 NOT TAKEN 0. 000 NOT TAKEN NOT TAKEN 0.019 NOT TAKEN NOT TAKEN 0.019 NOT TAKEN 0.018 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0. 000 0. 000 0. 000 SPILLED NOT TAKEN 0. 000 0. 000 NOT TAKEN 0. 000 0. 000 NOT TAKEN 0. 000 NOT TAKEN 0. 000 NOT TAKEN 0. 000 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.018 NOT TAKEN 0.018 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0. 000 0. 000 SPILLED NOT TAKEN 0. 000 0. 000 NOT TAKEN 0. 000 0. 000 NOT TAKEN 0. 000 NOT TAKEN 0. 000 NOT TAKEN 0. 000 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0. 020 NOT TAKEN 0. 020 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0. 000 NOT TAKEN SPILLED NOT TAKEN 0. 000 0. 000 NOT TAKEN 0. 000 0. 000 NOT TAKEN 0. 000 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.017 NOT TAKEN 0.017 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN SPILLED NOT TAKEN 0. 000 0. 000 NOT TAKEN 0. 000 NOT TAKEN NOT TAKEN 0. 000 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN SPILLED NOT TAKEN SPILLED Covance 6329-183 3M T-6295 PAGE: 70 1501 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 76 WEEK 80 WEEK 84 WEEK WEEK 92 WEEK 96 WEEK 100 WEEK 104 i i i CO 1 CO 1 1 GROUP: FEMALE 2 - 0.5 ]PPM C92939 C92940 C92941 C92942 C92943 C92944 C92945 C92946 C92947 C92948 C92949 C92950 C92951 C92952 C92953 C92954 C92955 C92956 C92957 C92958 C92959 C92960 C92961 C92962 C92963 C92964 C92965 C92966 C92967 C92968 C92969 NOT TAKEN 0.028 NOT TAKEN NOT TAKEN 0.019 NOT TAKEN NOT TAKEN 0. 020 0.021 0.019 NOT TAKEN NOT TAKEN 0.023 NOT TAKEN 0. 022 0.026 0.018 0. 020 0. 022 0.023 0. 022 0.019 0.018 NOT TAKEN 0.021 0.023 0.023 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN SPILLED NOT TAKEN NOT TAKEN 0.019 NOT TAKEN NOT TAKEN 0.018 0.024 0.021 NOT TAKEN NOT TAKEN 0.028 NOT TAKEN 0. 020 0. 022 0.018 0. 020 0. 022 0. 022 0.024 0.019 SPILLED NOT TAKEN 0. 022 0.025 0.004 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN SPILLED NOT TAKEN NOT TAKEN 0.011 NOT TAKEN NOT TAKEN 0. 020 0. 022 0.023 NOT TAKEN NOT TAKEN 0. 022 NOT TAKEN 0. 020 0.025 0.017 0.018 0. 020 0.016 0.025 0. 020 SPILLED NOT TAKEN 0.023 0.026 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.011 NOT TAKEN NOT TAKEN 0.018 0.017 0.023 NOT TAKEN NOT TAKEN 0.019 NOT TAKEN 0. 020 0.023 0.018 0.018 0. 022 0.015 0.024 0.019 0.017 NOT TAKEN 0.025 0. 022 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.011 NOT TAKEN NOT TAKEN 0.018 0.009 0. 020 NOT TAKEN NOT TAKEN 0. 022 NOT TAKEN 0.021 SPILLED NOT TAKEN 0.018 0.018 NOT TAKEN 0.024 SPILLED 0.014 NOT TAKEN SPILLED 0.021 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.015 NOT TAKEN NOT TAKEN 0.018 NOT TAKEN 0.003 NOT TAKEN NOT TAKEN 0.025 NOT TAKEN 0.019 0. 022 NOT TAKEN 0.016 SPILLED NOT TAKEN 0. 01 0 SPILLED SPILLED NOT TAKEN NOT TAKEN 0.019 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.019 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0. 022 NOT TAKEN 0.018 0. 001 NOT TAKEN 0.013 SPILLED NOT TAKEN NOT TAKEN SPILLED NOT TAKEN NOT TAKEN NOT TAKEN 0. 022 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.017 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0. 022 NOT TAKEN NOT TAKEN NOT TAKEN SPILLED NOT TAKEN NOT TAKEN SPILLED NOT TAKEN NOT TAKEN NOT TAKEN SPILLED NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN Covance 6329-183 3M T-6295 PAGE: 71 1502 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 76 WEEK 80 WEEK 84 WEEK 88 WEEK 92 WEEK 96 WEEK 100 WEEK 104 GROUP: FEMALE 2 - 0 . 5 PPM C92970 C92971 C92972 C92973 C92974 C92975 C92976 C92977 C92978 C92979 C92980 C92981 C92982 C92983 C92984 C92985 C92986 C92987 C92988 C92989 C92990 0.019 0.028 0.021 0. 020 0. 022 0.026 0.024 0.021 NOT TAKEN 0.018 NOT TAKEN NOT TAKEN NOT TAKEN 0. 020 0. 022 0.019 0. 022 NOT TAKEN 0.021 0.027 0. 022 0.019 0.027 0.021 0. 020 0. 022 0.028 0.021 0.023 NOT TAKEN 0. 020 NOT TAKEN NOT TAKEN NOT TAKEN 0.018 0. 022 0. 020 0.021 NOT TAKEN 0.018 0. 022 0. 020 GROUP: FEMALE 3 - 2 PPM C92991 C92992 C92993 C92994 C92995 C92996 C92997 0.091 0.083 NOT TAKEN 0.087 NOT TAKEN NOT TAKEN 0.085 0.090 0.077 NOT TAKEN 0.014 NOT TAKEN NOT TAKEN 0.068 0.018 0.027 0.025 0.021 0.023 0.027 0.017 0.023 NOT TAKEN 0.018 NOT TAKEN NOT TAKEN NOT TAKEN 0. 020 0. 022 0.017 0.024 NOT TAKEN 0. 020 NOT TAKEN 0.019 0.077 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.016 0.024 0. 022 0. 020 0.023 0.021 0.018 0. 022 NOT TAKEN 0. 001 NOT TAKEN NOT TAKEN NOT TAKEN 0.017 0.017 0.014 0.021 NOT TAKEN 0.018 NOT TAKEN 0.018 0.078 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.016 NOT TAKEN 0. 01 0 0.019 0. 022 0.024 0.015 0.021 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.019 0.016 0.012 0.021 NOT TAKEN 0.019 NOT TAKEN 0.019 0.077 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.016 NOT TAKEN NOT TAKEN 0.017 0.021 0. 020 0.016 0.019 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.017 0.018 0.007 0.019 NOT TAKEN 0.018 NOT TAKEN NOT TAKEN 0.079 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.007 NOT TAKEN NOT TAKEN 0.018 0.016 0.018 0.005 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.013 0.014 NOT TAKEN 0.021 NOT TAKEN 0. 020 NOT TAKEN NOT TAKEN 0.070 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0. 01 0 NOT TAKEN NOT TAKEN 0.012 0.018 0. 022 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.009 0.015 NOT TAKEN 0.021 NOT TAKEN 0.019 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN Covance 6329-183 3M T-6295 PAGE: 72 1503 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 76 WEEK 80 WEEK 84 WEEK 88 WEEK 92 WEEK 96 WEEK 100 WEEK 104 GROUP: FEMALE 3 - 2 PPM C92998 C92999 C93000 C93001 C93002 C93003 C93004 C93005 C93006 C93007 C93008 C93009 C93010 C93011 C93012 C93013 C93014 C93015 C93016 C93017 C93018 C93019 C93020 C93021 C93022 C93023 C93024 C93025 C93026 C93027 C93028 NOT TAKEN 0.074 NOT TAKEN 0.077 0.085 0.103 NOT TAKEN 0.081 NOT TAKEN 0.080 NOT TAKEN 0.078 0.079 0.075 0.066 0.046 NOT TAKEN 0.092 SPILLED 0.104 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.077 NOT TAKEN 0.083 0.075 0.113 0.079 0.091 NOT TAKEN 0.076 NOT TAKEN 0.038 0.026 0.089 NOT TAKEN 0.051 NOT TAKEN 0.076 NOT TAKEN 0.071 0.071 0.083 SPILLED 0.040 NOT TAKEN 0.091 0.109 0.094 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.074 NOT TAKEN 0.041 0.076 0.090 0.081 0.078 NOT TAKEN 0.073 NOT TAKEN NOT TAKEN NOT TAKEN 0.097 NOT TAKEN 0.103 NOT TAKEN 0.079 NOT TAKEN 0.073 0.060 0.082 NOT TAKEN 0.062 NOT TAKEN 0.097 NOT TAKEN 0.113 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.071 NOT TAKEN 0.074 0.078 0.091 0.061 0.060 NOT TAKEN 0.098 NOT TAKEN NOT TAKEN NOT TAKEN SPILLED NOT TAKEN 0.105 NOT TAKEN 0.085 NOT TAKEN 0.063 0.028 0.074 NOT TAKEN NOT TAKEN NOT TAKEN 0.088 NOT TAKEN 0.071 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.062 NOT TAKEN NOT TAKEN 0.077 0. 1 00 0.016 0.061 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN SPILLED NOT TAKEN NOT TAKEN NOT TAKEN 0.092 NOT TAKEN NOT TAKEN NOT TAKEN 0.073 NOT TAKEN NOT TAKEN NOT TAKEN 0.097 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.061 NOT TAKEN NOT TAKEN 0.076 0.096 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.072 NOT TAKEN NOT TAKEN NOT TAKEN 0.079 NOT TAKEN NOT TAKEN NOT TAKEN 0.071 NOT TAKEN NOT TAKEN NOT TAKEN 0.052 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.063 NOT TAKEN NOT TAKEN 0.071 0.078 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.056 NOT TAKEN NOT TAKEN NOT TAKEN 0.083 NOT TAKEN NOT TAKEN NOT TAKEN 0.073 NOT TAKEN NOT TAKEN NOT TAKEN 0.123 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.057 NOT TAKEN NOT TAKEN 0.064 0.087 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN Covance 6329-183 3M T-6295 PAGE: 73 1504 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 76 WEEK 80 WEEK 84 WEEK 88 WEEK 92 WEEK 96 WEEK 100 WEEK 104 GROUP: FEMALE 3 - 2 PPM C93029 C93030 C93031 C93032 C93033 C93034 C93035 C93036 C93037 C93038 C93039 C93040 C93041 C93042 C93043 C93044 C93045 C93046 C93047 C93048 C93049 C93050 NOT TAKEN 0.076 NOT TAKEN 0.045 NOT TAKEN 0.090 NOT TAKEN 0.076 NOT TAKEN NOT TAKEN 0.073 0.093 0.079 0.076 SPILLED NOT TAKEN NOT TAKEN 0.094 0.074 0.099 NOT TAKEN 0.084 NOT TAKEN 0.068 NOT TAKEN NOT TAKEN NOT TAKEN 0.114 NOT TAKEN 0.072 NOT TAKEN NOT TAKEN 0.067 0.107 0.048 0.078 SPILLED NOT TAKEN NOT TAKEN NOT TAKEN 0.071 0.092 NOT TAKEN 0.079 GROUP: FEMALE 4 - 5 PPM C93051 C93052 C93053 C93054 C93055 C93056 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.009 0.226 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.231 NOT TAKEN 0.073 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.047 NOT TAKEN NOT TAKEN 0.070 0. 1 00 NOT TAKEN 0.064 0.083 NOT TAKEN NOT TAKEN NOT TAKEN 0.068 0.090 NOT TAKEN 0.088 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.211 NOT TAKEN 0.062 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.069 0.106 NOT TAKEN 0.063 0.070 NOT TAKEN NOT TAKEN NOT TAKEN 0.065 0.076 NOT TAKEN 0. 1 00 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.201 NOT TAKEN 0.070 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.069 0.055 NOT TAKEN 0.044 0.073 NOT TAKEN NOT TAKEN NOT TAKEN 0.069 0.086 NOT TAKEN 0.089 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.136 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.084 0.131 NOT TAKEN NOT TAKEN SPILLED NOT TAKEN NOT TAKEN NOT TAKEN 0.067 0.087 NOT TAKEN 0.042 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.113 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.071 0.088 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.064 0.090 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN Covance 6329-183 3M T-6295 PAGE: 74 1505 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 76 WEEK 80 WEEK 84 WEEK 88 WEEK 92 WEEK 96 WEEK 100 WEEK 104 GROUP: C93057 C93058 C93059 C93060 C93061 C93062 C93063 C93064 C93065 C93066 C93067 C93068 C93069 C93070 C93071 C93072 C93073 C93074 C93075 C93076 C93077 C93078 C93079 C93080 C93081 C93082 C93083 C93084 C93085 C93086 C93087 FEMALE 4 - 5 PPM 0.214 NOT TAKEN 0.226 0.221 SPILLED 0.215 0.244 NOT TAKEN 0.135 SPILLED NOT TAKEN 0.256 0.204 NOT TAKEN NOT TAKEN 0.209 NOT TAKEN 0.182 NOT TAKEN 0.219 0.232 0.196 0.181 0.233 0.203 0.203 0.213 NOT TAKEN NOT TAKEN 0.105 0.199 0.221 NOT TAKEN 0.209 0.239 NOT TAKEN 0.231 0.208 NOT TAKEN 0.241 0.228 NOT TAKEN 0.224 0.184 NOT TAKEN NOT TAKEN 0.223 NOT TAKEN 0.068 NOT TAKEN 0.184 0.227 0.231 0.201 0.255 0.195 0.203 0.227 NOT TAKEN NOT TAKEN 0.143 0.226 0.224 NOT TAKEN 0.210 0.226 NOT TAKEN 0. 220 0.217 NOT TAKEN 0.311 0. 200 NOT TAKEN 0.251 0.217 NOT TAKEN NOT TAKEN 0.205 NOT TAKEN NOT TAKEN NOT TAKEN 0.219 0.223 0.212 0.211 SPILLED 0.197 0.227 0.248 NOT TAKEN NOT TAKEN 0.240 0.232 0. 220 NOT TAKEN 0.211 0.239 NOT TAKEN 0.219 0.207 NOT TAKEN 0.281 0.195 NOT TAKEN 0.237 0.237 NOT TAKEN NOT TAKEN 0. 202 NOT TAKEN NOT TAKEN NOT TAKEN 0.138 0.195 0.247 0.199 0.225 0.208 0.209 0.215 NOT TAKEN NOT TAKEN 0.234 0.219 0.141 NOT TAKEN 0.205 0.184 NOT TAKEN 0.234 0.214 NOT TAKEN SPILLED 0.194 NOT TAKEN 0.228 0.237 NOT TAKEN NOT TAKEN 0.205 NOT TAKEN NOT TAKEN NOT TAKEN 0.059 0.182 0.243 0.175 0.189 0.197 0.183 0.193 NOT TAKEN NOT TAKEN NOT TAKEN 0.231 0.171 NOT TAKEN 0.189 0.199 NOT TAKEN 0.234 0.210 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.239 0.246 NOT TAKEN NOT TAKEN 0. 200 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.176 0.243 0.192 SPILLED 0.201 0.044 0.192 NOT TAKEN NOT TAKEN NOT TAKEN 0.204 NOT TAKEN NOT TAKEN 0.189 0.152 NOT TAKEN 0.229 0.207 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.173 0.157 NOT TAKEN NOT TAKEN 0.197 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.164 0.249 0.204 SPILLED SPILLED NOT TAKEN 0.177 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.182 0.199 NOT TAKEN 0.203 0.167 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.212 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.179 0.245 0.183 0.265 0.207 NOT TAKEN 0.224 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN Covance 6329-183 3M T-6295 PAGE: 75 1506 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 76 WEEK 80 WEEK 84 WEEK 88 WEEK 92 WEEK 96 WEEK 100 WEEK 104 GROUP: FEMALE 4 - 5 PPM C93088 C93089 C93090 C93091 C93092 C93093 C93094 C93095 C93096 C93097 C93098 C93099 C93100 C93101 C93102 C93103 C93104 C93105 C93106 C93107 C93108 C93109 C93110 0.242 0.224 0.234 0.154 0.132 0.221 0.180 0.205 NOT TAKEN 0.232 0.196 0.209 0.216 0.218 0.230 0.224 0.006 0.221 0.212 0.184 NOT TAKEN NOT TAKEN 0.205 NOT TAKEN 0.227 0.209 NOT TAKEN NOT TAKEN 0.203 0.211 0.234 NOT TAKEN 0.211 0.195 0.183 0.217 0.182 0.254 0.223 0.267 SPILLED 0. 202 0.228 NOT TAKEN NOT TAKEN 0.226 GROUP: FEMALE 5 - 20 PPM C93111 C93112 C93113 C93114 C93115 NOT TAKEN NOT TAKEN 0.872 NOT TAKEN 1.001 NOT TAKEN NOT TAKEN 1.059 NOT TAKEN 0.923 NOT TAKEN 0.236 0.229 NOT TAKEN NOT TAKEN 0.194 0.206 0.210 NOT TAKEN 0.226 0.205 0.182 0.221 0. 200 0.265 0.169 NOT TAKEN 0.125 0. 202 0.223 NOT TAKEN NOT TAKEN 0.193 NOT TAKEN NOT TAKEN 0.898 NOT TAKEN 0.930 NOT TAKEN 0.240 0.215 NOT TAKEN NOT TAKEN 0.218 0.167 0.169 NOT TAKEN 0.230 0.182 0.148 0.208 0.192 0.254 0.192 NOT TAKEN SPILLED 0.195 0.217 NOT TAKEN NOT TAKEN 0.119 NOT TAKEN NOT TAKEN 0.957 NOT TAKEN 0. 868 NOT TAKEN 0.118 0.209 NOT TAKEN NOT TAKEN 0.213 0.191 0.244 NOT TAKEN 0.243 0.198 0.139 NOT TAKEN 0.212 SPILLED 0.183 NOT TAKEN NOT TAKEN 0.175 0.158 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.938 NOT TAKEN 0.865 NOT TAKEN NOT TAKEN 0.206 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.186 NOT TAKEN 0.196 0.180 NOT TAKEN NOT TAKEN 0.186 SPILLED 0.075 NOT TAKEN NOT TAKEN NOT TAKEN 0.205 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.716 NOT TAKEN 0.853 NOT TAKEN NOT TAKEN 0.198 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.180 NOT TAKEN NOT TAKEN 0.173 NOT TAKEN NOT TAKEN 0.194 SPILLED NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.201 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.836 NOT TAKEN 0.692 NOT TAKEN NOT TAKEN 0.143 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.028 NOT TAKEN NOT TAKEN 0.181 NOT TAKEN NOT TAKEN 0.211 0.286 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.201 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.849 NOT TAKEN 0.662 Covance 6329-183 3M T-6295 PAGE: 76 1507 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 76 WEEK 80 WEEK 84 WEEK 88 WEEK 92 WEEK 96 WEEK 100 WEEK 104 GROUP: FEMALE 5 - 2 0 PPM C93116 C93117 C93118 C93119 C93120 C93121 C93122 C93123 C93124 C93125 C93126 C93127 C93128 C93129 C93130 C93131 C93132 C93133 C93134 C93135 C93136 C93137 C93138 C93139 C93140 C93141 C93142 C93143 C93144 C93145 C93146 NOT TAKEN NOT TAKEN 1.075 1.229 NOT TAKEN 0.979 0.838 NOT TAKEN 1.024 1.058 NOT TAKEN NOT TAKEN 0.956 1.118 NOT TAKEN 0.852 0.858 0.993 NOT TAKEN SPILLED NOT TAKEN 0.945 NOT TAKEN 0.976 0.935 0.885 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 1.224 NOT TAKEN NOT TAKEN 0.904 1.363 NOT TAKEN NOT TAKEN 1.035 NOT TAKEN 1.047 1.148 NOT TAKEN NOT TAKEN 1.068 1.127 NOT TAKEN 0.727 0.805 0.916 NOT TAKEN SPILLED NOT TAKEN 1.140 NOT TAKEN 1.081 0.903 0.994 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 1.208 NOT TAKEN NOT TAKEN SPILLED 1.305 NOT TAKEN NOT TAKEN 1.052 NOT TAKEN 1.044 1.178 NOT TAKEN NOT T1A.0K2E2N 1.134 NOT TAKEN 0.958 0.831 0.928 NOT TAKEN SPILLED NOT TAKEN 1.026 NOT TAKEN 1.041 0.913 0.941 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 1.207 NOT TAKEN NOT TAKEN 1.309 1.268 NOT TAKEN NOT TAKEN 0.788 NOT TAKEN 1.408 1.033 NOT TAKEN NOT T1A.0K0E0N 0.953 NOT TAKEN 0.867 0.836 0.871 NOT TAKEN SPILLED NOT TAKEN 0.830 NOT TAKEN SPILLED 0.904 0.863 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 1.194 NOT TAKEN NOT TAKEN 1.213 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT T1A.1K2E1N 1.108 NOT TAKEN NOT TAKEN 0.958 0.693 NOT TAKEN 0.473 0.698 0.846 NOT TAKEN SPILLED NOT TAKEN 0.926 NOT TAKEN 0.953 0.912 0.877 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 1.071 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 1.161 1.093 NOT TAKEN NOT TAKEN 0.919 0.542 NOT TAKEN NOT TAKEN 0.835 0.919 NOT TAKEN SPILLED NOT TAKEN 0.614 NOT TAKEN 1.057 0.879 0.714 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 1.003 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 1.312 SPILLED NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 1.291 0.899 NOT TAKEN SPILLED NOT TAKEN 0.884 NOT TAKEN 1.036 0.852 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 1.054 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 1.178 SPILLED NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.020 0.901 NOT TAKEN 0.559 NOT TAKEN 0.758 NOT TAKEN 1.171 0.700 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.846 Covance 6329-183 3M T-6295 PAGE: 77 1508 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 76 WEEK 80 WEEK 84 WEEK 88 WEEK 92 WEEK 96 WEEK 100 WEEK 104 GROUP: FEMALE 5 - 2 0 PPM C93147 C93148 C93149 C93150 C93151 C93152 C93153 C93154 C93155 C93156 C93157 C93158 C93159 C93160 C93161 C93162 C93163 C93164 C93165 C93166 C93167 C93168 C93169 C93170 C93171 C93172 C93173 C93174 C93175 C93176 C93177 NOT TAKEN 0.977 0.884 1.177 1.117 NOT TAKEN 1.158 NOT TAKEN NOT TAKEN 0.464 0.896 0.757 NOT TAKEN NOT TAKEN 0.938 0.981 NOT TAKEN 1.198 0.955 0.845 NOT TAKEN 1.006 NOT TAKEN SPILLED NOT TAKEN 0.770 0.979 0.986 1.065 0.826 1.190 NOT TAKEN 1.048 0.917 1.231 1.123 NOT TAKEN 1.040 NOT TAKEN NOT TAKEN 1.602 0. 868 0.909 NOT TAKEN NOT TAKEN 0.692 0.952 NOT TAKEN 1.080 0.945 0.768 NOT TAKEN 1.002 NOT TAKEN 1.275 NOT TAKEN NOT TAKEN 0.889 1.027 0.952 0.736 1.366 NOT TAKEN 1.037 0.917 1.082 1.072 NOT TAKEN 1.145 NOT TAKEN NOT TAKEN 1.161 0.893 0.936 NOT TAKEN NOT TAKEN 0.951 0.983 NOT TAKEN 1.122 0.927 0.916 NOT TAKEN 1.048 NOT TAKEN 1.058 NOT TAKEN NOT TAKEN 0.805 SPILLED 0.985 0.567 1.146 NOT TAKEN 0.982 0.985 1.182 1.013 NOT TAKEN 1.108 NOT TAKEN NOT TAKEN NOT TAKEN 0.850 0.916 NOT TAKEN NOT TAKEN SPILLED 0.923 NOT TAKEN 1.073 0.795 0.754 NOT TAKEN 1.286 NOT TAKEN 0.756 NOT TAKEN NOT TAKEN 1.088 0.862 1.026 NOT TAKEN 0.980 NOT TAKEN NOT TAKEN 0.830 NOT TAKEN 1.049 NOT TAKEN 0.965 NOT TAKEN NOT TAKEN NOT TAKEN 0.805 0.863 NOT TAKEN NOT TAKEN 0.897 0.913 NOT TAKEN 1.168 0.897 0.822 NOT TAKEN 0.944 NOT TAKEN 0.741 NOT TAKEN NOT TAKEN 0.933 0. 868 NOT TAKEN NOT TAKEN 0.942 NOT TAKEN NOT TAKEN 0.945 NOT TAKEN 0.876 NOT TAKEN 1.223 NOT TAKEN NOT TAKEN NOT TAKEN 0.820 0.901 NOT TAKEN NOT TAKEN 0.875 0.844 NOT TAKEN 1.190 0.951 0.783 NOT TAKEN 0.898 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.794 SPILLED NOT TAKEN NOT TAKEN 1.040 NOT TAKEN NOT TAKEN 0.394 NOT TAKEN 0.948 NOT TAKEN 0.845 NOT TAKEN NOT TAKEN NOT TAKEN 0.753 0.925 NOT TAKEN NOT TAKEN 0.880 0.791 NOT TAKEN 0.907 0.822 0.824 NOT TAKEN 0.842 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.562 0.870 NOT TAKEN NOT TAKEN 1.100 NOT TAKEN NOT TAKEN 0.773 NOT TAKEN 0.784 NOT TAKEN SPILLED NOT TAKEN NOT TAKEN NOT TAKEN 0.761 0.859 NOT TAKEN NOT TAKEN 0.870 SPILLED NOT TAKEN 0.988 0.782 0.775 NOT TAKEN 0.911 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0. 866 NOT TAKEN NOT TAKEN 1.195 Covance 6329-183 3M T-6295 PAGE: 78 1509 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 76 WEEK 80 WEEK 84 WEEK 88 WEEK 92 WEEK 96 WEEK 100 WEEK 104 GROUP: FEMALE 5 - 2 0 PPM C93178 C93179 C93180 0.863 1.005 NOT TAKEN 0.841 1.049 NOT TAKEN GROUP: FEMALE 6 - 20 PPM C93181 C93182 C93183 C93184 C93185 C93186 C93187 C93188 C93189 C93190 C93191 C93192 C93193 C93194 C93195 C93196 C93197 C93198 C93199 C93200 C93201 C93202 C93203 C93204 C93205 0. 000 0. 000 0. 000 0. 000 0. 000 NOT TAKEN 0. 000 0. 000 0. 000 0. 000 0. 000 0. 000 NOT TAKEN 0. 000 NOT TAKEN 0. 000 0. 000 0. 000 NOT TAKEN 0. 000 0. 000 0. 000 0. 000 0. 000 0. 000 0. 000 0. 000 0. 000 0. 000 0. 000 NOT TAKEN 0. 000 0. 000 0. 000 0. 000 0. 000 0. 000 NOT TAKEN 0. 000 NOT TAKEN 0. 000 0. 000 0. 000 NOT TAKEN 0. 000 0. 000 0. 000 0. 000 0. 000 0. 000 0.901 0.995 NOT TAKEN 0. 000 0. 000 0. 000 0. 000 0. 000 NOT TAKEN 0. 000 0. 000 0. 000 0. 000 0. 000 0. 000 NOT TAKEN 0. 000 NOT TAKEN 0. 000 0. 000 0. 000 NOT TAKEN 0. 000 0. 000 0. 000 0. 000 0. 000 0. 000 0.864 1.033 NOT TAKEN 0. 000 0. 000 0. 000 0. 000 0. 000 NOT TAKEN 0. 000 0. 000 NOT TAKEN 0. 000 SPILLED 0. 000 NOT TAKEN 0. 000 NOT TAKEN NOT TAKEN SPILLED 0. 000 NOT TAKEN 0. 000 0. 000 0. 000 0. 000 SPILLED 0. 000 0.825 1.062 NOT TAKEN 0. 000 0. 000 0. 000 0. 000 0. 000 NOT TAKEN SPILLED 0. 000 NOT TAKEN 0. 000 NOT TAKEN 0. 000 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN SPILLED 0. 000 NOT TAKEN 0. 000 0. 000 0. 000 NOT TAKEN NOT TAKEN 0. 000 0.846 1.068 NOT TAKEN 0. 000 0. 000 0. 000 0. 000 0. 000 NOT TAKEN 0. 000 SPILLED NOT TAKEN 0. 000 NOT TAKEN 0. 000 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0. 000 0. 000 NOT TAKEN NOT TAKEN 0. 000 0. 000 NOT TAKEN NOT TAKEN 0. 000 0.749 0.831 NOT TAKEN 0. 000 0. 000 0. 000 0. 000 0. 000 NOT TAKEN 0. 000 SPILLED NOT TAKEN 0. 000 NOT TAKEN 0. 000 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0. 000 0. 000 NOT TAKEN NOT TAKEN 0. 000 0. 000 NOT TAKEN NOT TAKEN 0. 000 0.870 1.202 NOT TAKEN 0. 000 0. 000 SPILLED 0. 000 0. 000 NOT TAKEN NOT TAKEN 0. 000 NOT TAKEN 0. 000 NOT TAKEN 0. 000 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0. 000 0. 000 NOT TAKEN NOT TAKEN 0. 000 0. 000 NOT TAKEN NOT TAKEN 0. 000 Covance 6329-183 3M T-6295 PAGE: 79 1510 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER WEEK 76 WEEK 80 WEEK 84 WEEK 88 WEEK 92 WEEK 96 WEEK 100 WEEK 104 GROUP: C93206 C93207 C93208 C93209 C93210 C93211 C93212 C93213 C93214 C93215 C93216 C93217 C93218 C93219 C93220 FEMALE 6 - 2 0 PPM 0 .000 NOT TAKEN 0 .000 0 .000 0 .000 0 .000 0 .000 0 .000 0 .000 0 .000 0 .000 0 .000 0 .000 0 .000 0 .000 0 .000 NOT TAKEN 0 .000 0 .000 0 .000 0 .000 0 .000 0 .000 0 .000 0 .000 0 .000 0 .000 0 .000 0 .000 0 .000 0. 000 NOT TAKEN NOT TAKEN 0. 000 0. 000 0. 000 NOT TAKEN NOT TAKEN 0. 000 SPILLED 0. 000 0. 000 0. 000 0. 000 0. 000 0. 000 NOT TAKEN NOT TAKEN 0. 000 0. 000 0. 000 NOT TAKEN NOT TAKEN 0. 000 NOT TAKEN 0. 000 0. 000 0. 000 0. 000 0. 000 0. 000 NOT TAKEN NOT TAKEN 0. 000 0. 000 0. 000 NOT TAKEN NOT TAKEN 0. 000 NOT TAKEN 0. 000 NOT TAKEN NOT TAKEN 0. 000 0. 000 0. 000 NOT TAKEN NOT TAKEN SPILLED SPILLED 0. 000 NOT TAKEN NOT TAKEN SPILLED NOT TAKEN 0. 000 NOT TAKEN NOT TAKEN 0. 000 SPILLED 0. 000 NOT TAKEN NOT TAKEN 0. 000 0. 000 0. 000 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0. 000 NOT TAKEN NOT TAKEN 0. 000 0. 000 NOT TAKEN NOT TAKEN NOT TAKEN 0. 000 0. 000 0. 000 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0. 000 NOT TAKEN NOT TAKEN 0. 000 0. 000 Covance 6329-183 3M T-6295 PAGE: 80 1511 APPENDIX 5 Individual Clinical Hematology Data Individual Clinical Chemistry Data Individual Clinical Urinalysis Data Individual Urine Chemistry Data Covance 6329-183 3M T-6295 1512 Appendix 5 Individual Clinical Hematology Data Males Week 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL RBC HGB HCT MCV MCH MCHC PLT NUMBER X 106/m L G/DL % FL PG % X 103/m L Group: 1 C92505 C92506 C92524 C92526 C92529 C92542 C92543 C92546 C92548 C92553 MEAN S.D. N Group: 2 C92573 C92574 C92575 C92585 C92586 C92587 C92610 C92613 C92615 C92618 MEAN S.D. N Dose Level: 0 6.96 7.13 8.05 7.86 7.61 7.22 7.59 8.09 6.73 7.48 14.5 15.1 16.4 16.2 15.1 14.2 15.1 16.1 14.5 14.4 7.47 .459 10 15.2 .81 10 Dose Level: 0.5 7.14 8. 02 6.58 7.14 7.45 6.80 7.19 7.65 6.97 7.22 14.5 15.9 14.0 13.8 14.9 13.9 15.6 15.6 14.2 14.8 7.22 .414 10 14.7 .77 10 Dosage Unit: ppm T-6295 42.0 44.7 47.6 47.4 45.3 41.9 45.1 48.0 42.1 43.8 60.3 62.7 59.1 60.4 59.5 58.0 59.4 59.2 62.5 58.6 20.8 21.2 20.4 20.6 19.9 19.6 19.9 19.9 21.6 19.3 44.8 2.35 10 60.0 1.56 10 20.3 .73 10 Dosage Unit: ppm T-6295 42.5 47.6 40.8 41.4 44.1 40.7 46.0 45.8 41.6 43.9 59.5 59.4 62.1 58.0 59.2 59.8 64.0 59.9 59.7 60.7 20.4 19.9 21.3 19.3 19.9 20.5 21.6 20.4 20.3 20.5 43.4 2.43 10 60.2 1.69 10 20.4 .67 10 34.6 33.7 34.6 34.1 33.4 33.8 33.4 33.6 34.6 32.9 33.9 .59 10 34.2 33.4 34.4 33.3 33.7 34.2 33.8 34.0 34.1 33.7 33.9 .36 10 1086 1208 1021 941 1057 909 1181 914 1022 1145 1048 107 10 993 1028 1349 1138 1096 1033 1071 952 1022 1139 1082 111 10 Covance 6329-183 3M T-6295 1513 Appendix 5 Individual Clinical Hematology Data Males Week 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL RBC HGB HCT MCV MCH MCHC PLT NUMBER X106///L G/DL FL PG X 103///L Group: 3 C92632 C92646 C92650 C92652 C92657 C92668 C92676 C92678 C92684 C92686 MEAN S.D. N Group: 4 C92709 C92715 C92716 C92718 C92725 C92731 C92732 C92734 C92744 C92746 MEAN S.D. N Dose Level: 2. 0 6.27 8.26 8.07 7.80 7.21 7.93 7.59 7.82 7.57 6.90 13.6 17.3 16.2 16.5 14.5 15.6 15.0 15.3 14.7 14.7 7.54 .599 10 15.3 1.09 10 Dose Level: 5.0 7.39 8.70 6.87 7.81 6.91 7.89 6.93 7.39 7.71 6.66 15.0 16.6 14.4 16.4 14.7 16.3 14.3 16.0 16.2 13.8 7.43 .622 10 15.4 1.04 10 Dosage Unit: ppm T-6295 39.3 50.4 48.4 48.1 42.6 46.8 43.8 46.0 43.9 42.1 62.7 61.1 60.0 61.6 59.1 59.1 57.7 58.8 58.0 61.1 21.7 20.9 20.1 21.2 20.1 19.6 19.8 19.5 19.4 21.4 45.1 3.39 10 59.9 1.65 10 20.4 .85 10 Dosage Unit: ppm T-6295 43.8 49.2 40.9 47.9 43.2 48.0 41.9 46.9 48.0 40.5 59.3 56.5 59.5 61.3 62.5 60.8 60.5 63.5 62.3 60.7 20.3 19.1 20.9 21.0 21.2 20.7 20.7 21.7 21.0 20.8 45.0 3.32 10 60.7 1.97 10 20.7 .68 10 34.6 34.2 33.5 34.3 34.1 33.3 34.3 33.1 33.5 35.0 34.0 .61 10 34.2 33.8 35.2 34.2 34.0 34.0 34.1 34.1 33.7 34.2 34.1 .41 10 942 1075 1006 974 1048 1011 1040 968 1186 1033 1028 68. 10 998 1095 1043 956 994 794 1092 1076 1208 875 1013 118 10 Covance 6329-183 3M T-6295 1514 Appendix 5 Individual Clinical Hematology Data Males Week 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL RBC HGB HCT MCV MCH MCHC PLT NUMBER X106/iL G/DL % FL PG % X103/iL Group: 5 C92752 C92759 C92779 C92784 C92793 C92798 C92799 C92805 C92807 C92811 MEAN S.D. N Dose Level: 20.0 7.83 7.17 7.46 7.01 7.22 7.11 7.47 7.26 7.24 7.21 15.6 15.0 15.6 14.2 14.9 15.0 15.1 14.6 14.7 15.3 7.30 .234 10 15.0 .44 10 Dosage Unit: ppm T-6295 46.7 44.4 46.4 41.6 43.5 44.8 44.6 42.7 43.4 44.1 44.2 1.56 10 59.6 61.9 62.1 59.4 60.3 63.1 59.7 58.8 59.9 61.2 60.6 1.40 10 19.9 20.9 20.9 20.2 20.6 21.1 20.3 20.1 20.3 21.2 20.6 .45 10 33.5 33.8 33.6 34.1 34.2 33.4 33.9 34.2 33.9 34.6 33.9 .37 10 995 935 1224 1120 831 1051 1145 1170 999 1044 1051 118.1 10 Covance 6329-183 3M T-6295 1515 ANIMAL NUMBER Group: 1 C92505 C92506 C92524 C92526 C92529 C92542 C92543 C92546 C92548 C92553 MEAN S.D. N Group: 2 C92573 C92574 C92575 C92585 C92586 C92587 C92610 C92613 C92615 C92618 MEAN S.D. N Appendix 5 Individual Clinical Hematology Data Males Week 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS WBC X103///L N-SEG LYMPH X103///L X103//L MONO EOSIN X103///L X103//L BASO N-SEG% X 103//L LYMPH% MONO% EOSIN% Covance 6329-183 3M T-6295 BASO% Dose Level : 0 Dosage Unit: ppm T-6295 10.7 .6 9.2 .7 . 1 .0 6 86 6 1 0 10.7 1 . 2 8.8 .6 .0 .0 12 82 5 0 0 8.4 .8 7.0 .5 . 1 .0 9 84 5 1 0 12. 0 .6 10.7 .6 . 1 .0 5 89 5 1 0 8.8 .6 7.6 .5 . 1 .0 7 86 6 1 0 6. 6 .5 5.4 .6 . 1 .0 8 82 9 1 0 7.1 .9 5.7 .4 .0 .0 13 81 6 1 0 5.7 .2 5.1 .3 . 1 .0 4 89 6 1 0 9.0 1. 0 7.2 .6 . 1 .0 11 80 7 1 0 7.2 1. 0 5.1 .9 .2 .0 14 71 12 2 0 8. 6 .7 7.2 .6 . 1 .0 9 83 7 1 0 2.03 .30 1.92 .16 .06 .00 3..5 5..3 2 ..2 .5 .0 10 10 10 10 10 10 10 10 10 10 10 Dose Level : 0.5 Dosage Unit: ppm T-6295 7.8 .6 6. 6 .5 . 1 .0 8 85 6 1 0 7.1 .5 6.3 .2 .0 .0 8 90 2 1 0 15.1 4.9 8.8 1 . 2 . 1 .0 33 58 8 1 0 9.8 1 . 1 7.7 .8 . 1 .0 11 79 9 1 0 10.7 1.3 8. 6 .7 . 1 .0 12 80 7 1 0 8. 2 .9 6.4 .7 .2 .0 11 78 9 2 0 7.2 .6 6. 2 .3 .0 .0 9 86 4 1 0 8.5 .6 7.5 .3 .0 .0 8 88 3 1 0 1 1 . 2 .8 9.7 .6 .1 .0 8 86 5 1 0 7.0 .7 6.0 .3 . 1 .0 9 85 4 1 0 9.3 1 . 2 7.4 .6 . 1 .0 12 82 6 1 0 2.54 1.32 1.29 .31 .06 .00 7..6 9..1 2 .5 .3 .0 10 10 10 10 10 10 10 10 10 10 10 1516 ANIMAL NUMBER Group: 3 C92632 C92646 C92650 C92652 C92657 C92668 C92676 C92678 C92684 C92686 MEAN S.D. N Group: 4 C92709 C92715 C92716 C92718 C92725 C92731 C92732 C92734 C92744 C92746 MEAN S.D. N Appendix 5 Individual Clinical Hematology Data Males Week 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS WBC X103///L N-SEG LYMPH X103///L X103///L MONO EOSIN X103///L X103///L BASO N-SEG% X103///L LYMPH% MONO% EOSIN% Covance 6329-183 3M T-6295 BASO% Dose Level : 2. 0 Dosage Unit: ppm T-6295 12.9 1. 0 10.7 1.0 .1 .0 8 83 8 1 0 9.1 .5 8.0 .5 . 1 .0 6 88 6 1 0 5.6 .5 4.8 .2 .0 .0 8 86 4 1 0 7.7 .3 6.8 .4 . 1 .0 4 90 5 1 0 11.8 1.0 10.1 .6 .1 .0 8 86 5 1 0 8. 2 .5 7.4 .2 . 1 .0 7 90 3 1 0 9.4 .7 7.9 .7 .0 .0 8 84 7 0 0 7.2 .6 5.7 .8 . 1 .0 9 79 11 1 0 9.6 .7 7.9 .8 .2 .0 7 83 8 2 0 11.8 .8 10.2 .7 . 1 .0 6 86 6 1 0 9.3 .7 8.0 .6 . 1 .0 7 86 6 1 0 2.30 .23 1.94 .26 .06 .00 1.4 3.4 2.3 .5 .0 10 10 10 10 10 10 10 10 10 10 10 Dose Level: 5.0 Dosage Unit:: ppm T-6295 7.6 .8 5.3 .9 .5 .0 11 70 12 7 0 5.8 .4 5.1 .2 . 1 .0 6 88 4 2 0 6.8 .6 5.6 .5 . 1 .0 9 82 7 1 0 7.0 .5 6.3 .2 .0 .0 6 90 3 0 0 9.3 1. 0 7.4 .7 . 1 .0 11 80 8 1 0 5.6 .3 4.9 .3 .0 .0 5 89 5 1 0 8. 2 .7 7.1 .3 . 1 .0 8 87 4 1 0 7.7 .4 7.0 .3 .0 .0 5 90 4 1 0 7.9 .5 7.1 .2 .0 .0 7 90 3 0 0 6.3 .6 5.1 .5 . 1 .0 10 81 8 1 0 7.2 .6 6 . 1 .4 . 1 .0 8 85 6 2 0 1.15 . 21 .99 .24 .15 .00 2.3 6.5 2.9 2. 0 .0 10 10 10 10 10 10 10 10 10 10 10 1517 ANIMAL NUMBER Appendix 5 Individual Clinical Hematology Data Males Week 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS WBC X103///L N-SEG LYMPH X103///L X103//L MONO EOSIN X103///L X103//L BASO N-SEG% X103//L LYMPH% MONO% EOSIN% Covance 6329-183 3M T-6295 BASO% Group: 5 Dose Level : 20.0 Dosage Unit: ppm T-6295 C92752 C92759 C92779 C92784 C92793 C92798 C92799 C92805 C92807 C92811 5.6 6.7 132..19 6.5 ..68 1..84 .4 4.6 5.8 38..62 5.8 ...214 1..24 7.3 7.8 .4 .9 66..23 ..65 11.4 1.4 9.3 10.9 .9 9.0 11.4 .9 9.7 ..85 .8 ..........0002211111 ..........0000000000 111201 8862 83 15 72 76 12 12 8 8 89 87 79 82 83 85 11276 46 7 4 7 7 2211111111 0000000000 MEAN S.D. 8.4 2.87 .8 6.8 .46 2.19 .6 .38 .1 .07 ..000 102.8 83 4.8 6 2.4 1 .4 0.0 N 10 10 10 10 10 10 10 10 10 10 10 1518 Appendix 5 Individual Clinical Hematology Data Males Week 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ANISO POLY POIK HYPO TOXNEUT Group: 1 C92505 C92506 C92524 C92526 C92529 C92542 C92543 C92546 C92548 C92553 Group: 2 C92573 C92574 C92575 C92585 C92586 C92587 C92610 C92613 C92615 C92618 Dose Level: 0 -------------------- Dose Level: 0.5 --------------------- Dosage Unit: ppm T-6295 - - Dosage Unit: ppm T-6295 ----------- Covance 6329-183 3M T-6295 1519 Appendix 5 Individual Clinical Hematology Data Males Week 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ANISO POLY POIK HYPO TOXNEUT Group: 3 C92632 C92646 C92650 C92652 C92657 C92668 C92676 C92678 C92684 C92686 Group: 4 C92709 C92715 C92716 C92718 C92725 C92731 C92732 C92734 C92744 C92746 Dose Level: 2.0 -------------------- Dose Level: 5.0 --------------------- Dosage Unit: ppm T-6295 - - Dosage Unit: ppm T-6295 ----------- Covance 6329-183 3M T-6295 1520 Appendix 5 Individual Clinical Hematology Data Males Week 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ANISO POLY POIK HYPO TOXNEUT Group: 5 C92752 C92759 C92779 C92784 C92793 C92798 C92799 C92805 C92807 C92811 Dose Level: 20.0 --------------------- Dosage Unit: ppm T-6295 ----------- Covance 6329-183 3M T-6295 1521 Appendix 5 Individual Clinical Hematology Data Females Week 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL RBC HGB HCT MCV MCH MCHC PLT NUMBER X 1 0 6//2L G/DL FL PG X 103//2L Group: 1 C92861 C92864 C92876 C92877 C92882 C92883 C92914 C92919 C92925 C92926 MEAN S.D. N Group: 2 C92941 C92945 C92950 C92953 C92959 C92964 C92968 C92969 C92976 C92982 MEAN S.D. N Dose Level: 0 7.48 6.77 7.39 7.18 7.13 7.26 7.62 6.97 7.43 7.61 15.5 14.4 15.7 15.4 15.2 15.0 15.2 15.0 14.6 15.8 7.28 .276 10 15.2 .45 10 Dose Level: 0.5 6.97 7.38 7.78 6.99 6.94 7.48 7.32 7.36 7.74 8.01 14.6 15.2 16.3 14.6 14.6 15.9 15.0 15.8 15.4 15.8 7.40 .367 10 15.3 .62 10 Dosage Unit: ppm T-6295 46.1 42.8 46.4 45.5 45.3 45.2 44.2 44.1 44.4 46.7 45.1 1.20 10 61.6 63.2 62.9 63.4 63.6 62.3 58.1 63.3 59.8 61.4 62.0 1.80 10 20.7 21.3 21.2 21.4 21.3 20.7 19.9 21.5 19.7 20.7 20.8 .63 10 Dosage Unit: ppm T-6295 43.6 45.0 47.7 44.1 43.9 46.7 45.6 46.0 46.9 47.1 62.5 60.9 61.3 63.1 63.2 62.4 62.4 62.4 60.6 58.8 20.9 20.6 20.9 20.9 21.0 21.3 20.6 21.5 19.8 19.7 45.7 1.46 10 61.8 1.36 10 20.7 .58 10 33.6 33.8 33.8 33.8 33.5 33.1 34.3 34.0 32.9 33.8 33.7 .41 10 33.4 33.9 34.1 33.1 33.3 34.1 32.9 34.4 32.7 33.5 33.5 .57 10 1199 983 1058 874 1146 1222 1083 1208 1103 1153 1103 109. 10 1182 1595 1061 1095 983 955 1106 1187 1069 1023 1126 181. 10 Covance 6329-183 3M T-6295 1522 Appendix 5 Individual Clinical Hematology Data Females Week 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL RBC HGB HCT MCV MCH MCHC PLT NUMBER X 1 0 6/^L G/DL % FL PG % X 103/m L Group: 3 C92993 C92996 C92998 C93004 C93006 C93008 C93023 C93031 C93033 C93045 MEAN S.D. N Group: 4 C93058 C93067 C93070 C93074 C93075 C93083 C93084 C93089 C93094 C93105 MEAN S.D. N Dose Level: 2.0 7.25 7.84 7.43 7.72 7.82 7.65 7.76 6.79 6.99 7.66 14.8 16.0 14.8 15.1 15.4 16.2 15.1 14.6 13.8 15.6 7.49 .367 10 15.1 .70 10 Dose Level: 5.0 8.08 7.30 7.37 6.98 8.01 7.00 7.69 7.33 7.21 7.08 15.7 15.8 15.5 15.3 16.2 15.0 15.5 15.2 14.8 15.1 7.40 .396 10 15.4 .42 10 Dosage Unit: ppm T-6295 44.5 46.6 45.4 45.6 45.5 47.4 45.4 43.0 42.1 46.7 45.2 1.64 10 61.3 59.4 61.2 59.1 58.2 61.9 58.5 63.3 60.2 60.9 60.4 1.62 10 20.3 20.4 20.0 19.6 19.7 21.1 19.5 21.4 19.7 20.4 20.2 .64 10 Dosage Unit: ppm T-6295 47.2 45.8 46.5 45.8 47.2 44.7 46.1 45.5 45.0 44.2 58.4 62.8 63.1 65.7 58.9 63.8 60.0 62.0 62.4 62.4 19.4 21.6 21.1 22.0 20.2 21.4 20.1 20.8 20.5 21.3 45.8 1.00 10 62.0 2.25 10 20.8 .79 10 33.2 34.3 32.7 33.1 33.8 34.1 33.2 33.9 32.8 33.5 33.5 .55 10 33.2 34.5 33.3 33.5 34.3 33.5 33.6 33.5 32.8 34.1 33.6 .52 10 973 1189 1096 1273 951 1127 1175 856 1068 979 1069 128 10 1324 1085 1046 893 1091 1023 1211 1115 1181 1066 1104 116 10 Covance 6329-183 3M T-6295 1523 Appendix 5 Individual Clinical Hematology Data Females Week 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL RBC HGB HCT MCV MCH MCHC PLT NUMBER X106//2L G/DL FL PG X103//2L Group: 5 C93114 C93123 C93142 C93144 C93151 C93155 C93158 C93159 C93164 C93175 MEAN S.D. N Dose Level: 20.0 7.27 7.19 7.54 7.36 7.57 7.13 6.97 7.38 7.68 7.66 15.2 15.1 15.0 15.6 15.3 15.6 15.0 15.3 15.3 15.8 7.38 .238 10 15.3 .27 10 Dosage Unit: ppm T-6295 44.5 45.2 45.5 46.3 46.4 45.3 44.0 45.3 46.1 47.8 61.3 62.9 60.4 62.9 61.3 63.5 63.1 61.5 60.0 62.5 21.0 21.0 19.9 21.3 20.2 21.8 21.5 20.7 20.0 20.7 45.6 1.07 10 61.9 1.21 10 20.8 .64 10 34.2 33.3 32.9 33.8 33.0 34.4 34.1 33.7 33.3 33.1 33.6 .53 10 1171 1092 1015 1155 1006 1022 1193 1042 1269 1112 1108 88.4 10 Covance 6329-183 3M T-6295 1524 ANIMAL NUMBER Group: 1 C92861 C92864 C92876 C92877 C92882 C92883 C92914 C92919 C92925 C92926 MEAN S.D. N Group: 2 C92941 C92945 C92950 C92953 C92959 C92964 C92968 C92969 C92976 C92982 MEAN S.D. N Appendix 5 Individual Clinical Hematology Data Females Week 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS WBC X103//2L N-SEG LYMPH X103//2L X103//2L MONO EOSIN X103/iL X103/iL BASO N-SEG% X103/iL LYMPH% MONO% EOSIN% Covance 6329-183 3M T-6295 BASO% Dose Level : 0 Dosage Unit: ppm T-6295 7.6 .7 6.5 .3 .1 .0 9 6.6 .5 5.9 .1 .1 .0 8 4.6 .7 3.6 .1 .2 .0 15 6.4 .9 5.2 .3 .0 .0 14 4.5 .5 3.7 .2 .1 .0 11 7.7 .8 6.2 .6 .1 .0 11 8.9 .8 7.8 .2 .0 .0 9 11.7 1.0 10.2 .5 .1 .0 8 9.9 .4 8.8 .6 .1 .0 4 6.2 .3 5.2 .5 .1 .0 5 7.4 .7 6.3 .3 .1 .0 9 2.27 .23 2 . 12 .20 .06 .00 3 10 10 10 10 10 10 10 Dose Level : 0.5 Dosage Unit: ppm T-6295 5.5 1 . 1 4.2 .2 .0 .0 20 8 . 1 .6 7.1 .3 .1 .0 8 7.3 2.8 4.3 .2 .0 .0 38 6 . 1 .3 5.7 .1 .1 .0 4 5.6 .2 5.1 .2 .0 .0 4 7.8 .3 6.9 .5 .1 .0 4 6.7 .4 5.8 .4 .1 .0 6 6.2 .4 5.5 .2 .0 .0 7 6.9 .4 5.9 .4 .1 .0 6 5.1 .4 4.5 .1 .0 .0 7 6.5 .7 5.5 .3 .0 .0 10 1 . 0 1 .78 1 . 0 1 .13 .05 .00 10 10 10 10 10 10 10 10 86 89 77 81 82 80 88 87 88 84 84 4.0 10 76 87 58 94 91 89 86 89 85 89 84 10.4 10 4 1 3 5 5 8 3 4 6 8 5 2.2 10 3 3 3 1 4 6 6 3 6 3 4 1.7 10 1 1 5 1 2 1 0 1 1 2 2 1.4 10 1 2 1 1 1 1 1 0 2 1 1 .6 10 0 0 0 0 0 0 0 0 0 0 0 .0 10 0 0 0 0 0 0 0 0 0 0 0 .0 10 1525 ANIMAL NUMBER Group: 3 C92993 C92996 C92998 C93004 C93006 C93008 C93023 C93031 C93033 C93045 MEAN S.D. N Group: 4 C93058 C93067 C93070 C93074 C93075 C93083 C93084 C93089 C93094 C93105 MEAN S.D. N Appendix 5 Individual Clinical Hematology Data Females Week 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS WBC X103//2L N-SEG LYMPH X103//2L X103//2L MONO EOSIN X103/iL X103/iL BASO N-SEG% X103/iL LYMPH% MONO% EOSIN% Covance 6329-183 3M T-6295 BASO% Dose Level : 2.0 Dosage Unit: ppm T-6295 6 . 1 .7 5.0 .4 .0 .0 11 8.2 .2 7.7 .3 .1 .0 2 3.4 .5 2.8 .1 .0 .0 14 2.3 .4 1 . 6 .3 .0 .0 16 4.4 .2 4.1 .1 .0 .0 4 7.0 .4 6.3 .2 .0 .0 5 4.4 .5 3.4 .3 .1 .0 12 6.0 .3 5.4 .3 .1 .0 5 3.6 .5 2.8 .2 .1 .0 15 4.5 .4 4.0 .1 .0 .0 8 5.0 .4 4.3 .2 .0 .0 9 1.80 .15 1.83 . 1 1 .05 .00 5 10 10 10 10 10 10 10 Dose Level : 5.0 Dosage Unit: ppm T-6295 5.0 .6 4.0 .2 .1 .0 12 4.8 .4 4.2 .1 .0 .0 8 5.1 .4 4.5 .1 .1 .0 7 13.0 1. 0 11.5 .4 .1 .0 8 6 . 1 .3 5.6 .2 .0 .0 6 3.4 .3 2.7 .3 .1 .0 9 7.9 .5 7.0 .4 .1 .0 6 9.2 .8 8.0 .3 .1 .0 9 5.3 .3 4.7 .2 .1 .0 6 9.0 .5 8 . 1 .3 .1 .0 5 6.9 .5 6.0 .2 .1 .0 8 2.88 .23 2.62 . 1 1 .04 .00 2 10 10 10 10 10 10 10 82 93 82 69 93 90 78 90 77 88 84 7.9 10 81 88 88 88 91 80 88 86 90 90 87 3.7 10 6 3 3 14 2 3 7 4 6 3 5 3.5 10 4 3 3 3 2 8 5 3 4 4 4 1.7 10 0 1 1 1 1 1 2 1 2 1 1 .6 10 2 1 2 1 0 2 1 1 1 1 1 .6 10 0 0 0 0 0 0 0 0 0 0 0 .0 10 0 0 0 0 0 0 0 0 0 0 0 .0 10 1526 ro LD co o PI (uN) 0I 01 E-i 2ro ro <D U > O U Individual Clinical Hematology Data o oooooooooo o o oo\ P ui C m I o\ I P' uoi H H MONO % s U o O 04 O P J h 04 H ^ Ui i-H Eh Sc B* ^p XM B 1/1 o\ tu C2U X P i i11l 11i SEG% s EMh B^ s i! U. P | g 3. | X ( CD & oH(J O' O ui ^o ii <|H PQX 111i [> OPPPPCNCNPCNCN P O rH ( N '^ C N ^ ' X ' ^ C n ^ C N * ^ rH st1 (N O rH ro^'X'OLD^'X'O'X'CO ococoococoi>ococo CO P Lnoo'X' OocNLnoLD rHrH rHrH rH [> o o o o o o o o o o o ooo ............... rH CARCII SALT CO 1--d) rti B a) h Q8 2P 3^ HwH K,w cc P Eh P PHHJ^i 1 11 Wq H 11 wx ; i P o Ol u) Ri O rlrlrlrlrlrlO rlrl ............................................................... UB * XQ S P, *! O Po 1i B P P .C.N.C.N.^.^.C.N.P.C..N .P .C.N U^ O2 rH X 111 -H d P rHOO rH ( N HOrH 2 a) Ej oU oJ- P P ffi 3. 11 e2u \ I i Oi rti C0O (NC0l>C0(NO^C0'>'> ............... ^rOLDOLD^^^Ln^ cEh H X P ^1X-1 i 11i O! ^ I> 0 rH LDH X H H & P [> roLT)l>'X'Lr)Lr)l>roi>ro in h o O H 3- i i Wo P P X 11 | O O Ol ............... rH O rH 1 1--1 d) >O P1 d) P 0 .' .>.' .>.O.H.c.o.c .o r.o.L.r .) (.N c n c o roH UPPQ--oOrH7 X 11! 11 d) C0O ')^')0')^LnLn'>Ln rH U) H CU i Hi P2Q i i Pi Pi LD d 0 U O P P ro OPl Ol P P P P P P P P CO P P o LT) P U) P P [> P ppppppPPpp o o o o o o o Oo o UUUUUUUUUU 1527 Appendix 5 Individual Clinical Hematology Data Females Week 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ANISO POLY POIK HYPO TOXNEUT Group: 1 C92861 C92864 C92876 C92877 C92882 C92883 C92914 C92919 C92925 C92926 Group: 2 C92941 C92945 C92950 C92953 C92959 C92964 C92968 C92969 C92976 C92982 Dose Level: 0 -------------------- Dose Level: 0.5 --------------------- Dosage Unit: ppm T-6295 - - Dosage Unit: ppm T-6295 ----------- Covance 6329-183 3M T-6295 1528 Appendix 5 Individual Clinical Hematology Data Females Week 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ANISO POLY POIK HYPO TOXNEUT Group: 3 C92993 C92996 C92998 C93004 C93006 C93008 C93023 C93031 C93033 C93045 Group: 4 C93058 C93067 C93070 C93074 C93075 C93083 C93084 C93089 C93094 C93105 Dose Level: 2.0 --------------------- Dose Level: 5.0 Dosage Unit: ppm T-6295 ----------- Dosage Unit: ppm T-6295 Covance 6329-183 3M T-6295 1529 Appendix 5 Individual Clinical Hematology Data Females Week 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ANISO POLY POIK HYPO TOXNEUT Group: 5 C93114 C93123 C93142 C93144 C93151 C93155 C93158 C93159 C93164 C93175 Dose Level: 20.0 ----------- Dosage Unit: ppm T-6295 --------------------- Covance 6329-183 3M T-6295 1530 Appendix 5 Individual Clinical Hematology Data Males Week 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL RBC HGB HCT MCV MCH MCHC PLT NUMBER X106/^L G/DL % FL PG % X 103/m L Group: 1 C92508 C92509 C92511 C92521 C92527 C92528 C92532 C92535 C92537 C92562 MEAN S.D. N Group: 2 C92583 C92593 C92598 C92600 C92612 C92616 C92621 C92624 C92627 C92629 MEAN S.D. N Dose Level: 0 8.63 8.60 8.93 8.84 7.98 8.39 9.27 8.69 7.74 8.46 15.1 15.9 16.7 15.8 15.4 15.3 16.7 15.3 14.5 15.8 8.55 .446 10 15.6 .69 10 Dose Level: 0.5 8.56 9.75 8.77 8.90 8.69 8.87 8.09 8.98 8.80 8.32 15.4 17.1 15.4 16.4 15.5 15.9 15.5 15.8 15.9 15.3 8.77 .441 10 15.8 .56 10 Dosage Unit: ppm T-6295 45.7 46.3 48.0 45.7 45.2 44.7 48.5 45.5 42.0 47.1 53.0 53.8 53.7 51.7 56.6 53.3 52.3 52.4 54.3 55.6 17.5 18.5 18.7 17.9 19.3 18.2 18.0 17.6 18.7 18.6 45.9 1.83 10 53.7 1.52 10 18.3 .56 10 Dosage Unit: ppm T-6295 46.7 49.8 46.2 48.4 45.3 46.5 46.0 47.2 45.9 45.0 54.5 51.1 52.6 54.4 52.1 52.4 56.9 52.6 52.2 54.1 18.0 17.6 17.6 18.5 17.8 17.9 19.1 17.6 18.1 18.3 46.7 1.45 10 53.3 1.68 10 18.0 .48 10 33.1 34.4 34.7 34.6 34.2 34.1 34.5 33.5 34.4 33.5 34.1 .55 10 32.9 34.4 33.4 33.9 34.2 34.2 33.6 33.5 34.7 33.9 33.9 .53 10 940 1103 1188 1157 1078 1533 1165 1173 1147 940 1142 164 10 1088 1141 1143 1140 1156 1135 1093 907 1218 904 1092 104 10 Covance 6329-183 3M T-6295 1531 Appendix 5 Individual Clinical Hematology Data Males Week 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL RBC HGB HCT MCV MCH MCHC PLT NUMBER X106/m L G/DL % FL PG % X 103/m L Group: 3 C92636 C92640 C92644 C92645 C92651 C92661 C92662 C92676 C92682 C92684 MEAN S.D. N Group: 4 C92711 C92713 C92714 C92716 C92719 C92722 C92726 C92728 C92741 C92748 MEAN S.D. N Dose Level: 2.0 8.63 9.12 7.99 9.01 8.78 8.30 8.79 9.04 7.97 9.22 8.68 .456 10 16.0 15.9 14.6 16.7 15.7 15.8 15.8 15.8 14.8 15.8 15.7 .60 10 Dose Level: 5.0 8.48 9.13 9.14 7.64 8.20 8.76 8.74 8.82 8.71 8.06 14.9 16.3 15.8 14.0 15.2 15.1 15.3 15.8 15.2 14.8 8.57 .478 10 15.2 .63 10 Dosage Unit: ppm T-6295 47.2 46.4 43.4 48.6 47.7 47.7 46.8 46.9 43.9 46.1 54.6 50.9 54.3 54.0 54.4 57.5 53.2 51.9 55.1 50.0 18.5 17.4 18.2 18.5 17.9 19.0 18.0 17.5 18.6 17.1 46.5 1.65 10 53.6 2.19 10 18.1 .60 10 Dosage Unit: ppm T-6295 44.9 47.5 46.1 41.4 44.8 44.6 46.2 46.2 45.3 44.9 53.0 52.1 50.5 54.3 54.7 51.0 52.8 52.4 52.0 55.7 17.6 17.9 17.3 18.3 18.6 17.3 17.5 17.9 17.4 18.4 45.2 1.61 10 52.8 1.64 10 17.8 .48 10 33.9 34.3 33.5 34.3 33.0 33.0 33.8 33.7 33.7 34.2 33.7 .47 10 33.3 34.4 34.2 33.7 34.0 33.9 33.1 34.2 33.5 33.0 33.7 .49 10 1090 1165 1156 1507 1422 905 1111 1161 922 1239 1168 189 10 1091 1319 1025 1062 1161 1146 885 1279 839 977 1078 155 10 Covance 6329-183 3M T-6295 1532 Appendix 5 Individual Clinical Hematology Data Males Week 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL RBC HGB HCT MCV MCH MCHC PLT NUMBER X106/iL G/DL % FL PG % X103/iL Group: 5 C92754 C92765 C92767 C92774 C92777 C92784 C92789 C92799 C92812 C92820 MEAN S.D. N Dose Level: 20.0 7.96 8.28 8.11 7.96 8.09 8.21 8.67 8.32 8.30 8.41 15.1 14.9 14.8 14.7 15.1 14.3 15.9 15.5 14.7 15.8 8.23 .216 10 15.1 .51 10 Dosage Unit: ppm T-6295 45.1 44.2 43.8 44.1 43.9 42.8 46.5 45.2 43.4 46.8 56.6 53.4 54.0 55.5 54.2 52.1 53.7 54.4 52.4 55.6 19.0 18.0 18.3 18.5 18.7 17.4 18.4 18.7 17.7 18.8 44.6 1.30 10 54.2 1.42 10 18.4 .51 10 33.5 33.7 33.8 33.4 34.5 33.5 34.3 34.3 33.8 33.8 33.9 .38 10 1098 1287 941 1000 1061 1124 1139 1328 1261 1147 1139 124.3 10 Covance 6329-183 3M T-6295 1533 ANIMAL NUMBER Group: 1 C92508 C92509 C92511 C92521 C92527 C92528 C92532 C92535 C92537 C92562 MEAN S.D. N Group: 2 C92583 C92593 C92598 C92600 C92612 C92616 C92621 C92624 C92627 C92629 MEAN S.D. N Appendix 5 Individual Clinical Hematology Data Males Week 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS WBC X103///L N-SEG LYMPH X103///L X103//L MONO EOSIN X103///L X103//L BASO N-SEG% X103/iL LYMPH% MONO% EOSIN% Covance 6329-183 3M T-6295 BASO% Dose Level : 0 Dosage Unit: ppm T-6295 6.2 .7 4.9 .5 .1 .0 12 79 8 1 0 7.0 .8 5.4 .7 .1 .0 12 77 9 1 0 7.2 .7 5.5 .8 .1 .0 10 76 12 2 0 5.1 .8 3.6 .5 .1 .0 17 71 10 2 0 9.2 1 . 1 6.9 1. 0 .2 .0 12 75 11 2 0 1 1 . 8 1.3 9.0 1 . 2 .2 .1 11 76 10 2 0 9.4 1 . 6 7.0 .7 .2 .0 17 74 7 2 0 7.6 1.3 5.5 .6 .2 .0 17 72 8 2 0 9.1 1. 0 6.6 1 . 2 .2 .0 11 73 14 2 0 9.4 1.7 6.4 1. 0 .3 .0 18 68 10 3 0 8.2 1 . 1 6 . 1 .8 .2 .0 14 74 10 2 0 1.95 .37 1.46 .27 .07 .03 3.1 3.2 2 . 1 .6 .0 10 10 10 10 10 10 10 10 10 10 10 Dose Level: 0.5 Dosage Unit: ppm T-6295 7.9 1.5 5.3 .9 .1 .0 19 68 11 2 0 7.7 .7 6.3 .5 .1 .0 9 83 7 1 0 10.0 1.3 7.3 1 . 1 .3 .0 13 73 11 3 0 9.0 1.4 6.7 .9 .0 .0 15 74 10 0 0 10.2 1. 0 7.9 1. 0 .2 .0 10 78 10 2 0 6.9 1 . 2 4.8 .7 .1 .0 18 71 10 2 0 7.8 1 . 1 5.7 .7 .3 .0 14 73 9 4 0 9.8 1 . 6 7.0 1. 0 .2 .0 16 71 10 2 0 8.7 1 . 6 6.3 .6 .1 .0 19 72 7 1 0 7.2 1.3 4.9 .9 .1 .0 18 68 12 2 0 8.5 1.3 6.2 .8 .2 .0 15 73 10 2 0 1.20 .28 1.04 .19 .10 .00 3.6 4.5 1 . 6 1 . 1 .0 10 10 10 10 10 10 10 10 10 10 10 1534 ANIMAL NUMBER Group: 3 C92636 C92640 C92644 C92645 C92651 C92661 C92662 C92676 C92682 C92684 MEAN S.D. N Group: 4 C92711 C92713 C92714 C92716 C92719 C92722 C92726 C92728 C92741 C92748 MEAN S.D. N Appendix 5 Individual Clinical Hematology Data Males Week 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS WBC X103///L N-SEG LYMPH X103///L X103//L MONO EOSIN X 103//L X103//L BASO N-SEG% X103/iL LYMPH% MONO% EOSIN% Covance 6329-183 3M T-6295 BASO% Dose Level : 2. 0 Dosage Unit: ppm T-6295 7.9 1 . 2 5.8 .7 .2 .0 15 73 9 2 0 8.0 1 . 1 5.9 .7 .2 .0 14 74 9 2 0 6.7 1 . 1 4.8 .7 . 1 .0 16 71 11 2 0 8.0 1. 0 6.6 .3 .1 .0 12 82 4 2 0 10.9 1.7 7.9 1 . 1 .2 .0 16 72 10 2 0 9.6 1.7 6.5 1 . 2 . 1 . 1 18 68 12 1 1 5.2 .7 3.6 .7 . 1 .0 14 70 14 2 0 7.0 1 . 1 5.2 .6 . 1 .0 16 74 9 1 0 8.7 1 . 2 6. 2 1 . 1 .2 .0 14 70 13 2 0 6.4 .8 4.7 .5 .3 .0 13 74 8 5 0 7.8 1 . 2 5.7 .8 .2 .0 15 73 10 2 0 1.64 .33 1. 20 .29 .07 .03 1 . 8 3.8 2. 8 1 . 1 .3 10 10 10 10 10 10 10 10 10 10 10 Dose Level: 5.0 Dosage Unit: ppm T-6295 8.4 1.7 5.7 .8 .2 .0 20 68 10 2 0 5.1 .9 3.7 .4 . 1 .0 18 72 7 2 0 6. 6 .7 5.0 .8 . 1 .0 11 75 12 2 0 6.4 1 . 1 4.7 .4 . 1 .0 17 74 7 2 0 6.7 1.3 4.4 .8 .2 .0 20 65 12 3 0 8.9 1.7 6.0 1. 0 .2 .0 19 68 11 2 0 8.0 1. 0 6. 2 .5 .2 .0 13 78 6 3 0 8.7 1.7 6.0 .8 . 1 .0 20 69 10 2 0 1 2. 6 .9 10. 8 .6 .2 .0 7 86 5 1 0 6.5 1 . 2 4.6 .6 . 1 .0 18 71 9 2 0 7.8 1 . 2 5.7 .7 .2 .0 16 73 9 2 0 2.08 .37 1.97 .20 .05 .00 4.5 6 . 1 2.5 .6 .0 10 10 10 10 10 10 10 10 10 10 10 1535 ANIMAL NUMBER Group: 5 C92754 C92765 C92767 C92774 C92777 C92784 C92789 C92799 C92812 C92820 MEAN S.D. N Appendix 5 Individual Clinical Hematology Data Males Week 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS WBC X103///L N-SEG LYMPH X103///L X103//L MONO EOSIN X103///L X103//L BASO N-SEG% X 103//L LYMPH% MONO% EOSIN% Covance 6329-183 3M T-6295 BASO% Dose Level : 20.0 Dosage Unit: ppm T-6295 10. 2 1.3 7.5 1 . 2 .2 .0 13 74 11 2 0 11.5 1 . 8 8.7 .8 .2 .0 16 76 7 2 0 18.9 1.5 16.0 1.2 .1 .1 8 84 7 1 0 7.8 1 . 2 4.8 1.5 .3 .0 15 62 19 3 0 7.6 1.5 5.8 .3 . 1 .0 19 76 4 1 0 13.1 2 . 1 9.4 1 . 2 .3 .0 16 72 10 2 0 8.5 1.4 6.3 .6 . 1 .0 17 75 7 1 0 6.0 1 . 6 3.8 .5 . 1 .0 27 62 8 2 0 9.7 2. 2 6.7 .6 .2 .0 22 70 6 2 0 11.5 1 . 2 9.4 .7 . 1 .0 11 82 6 1 0 10.5 1 . 6 7.8 .9 .2 .0 16 73 8 2 0 3.65 .35 3.43 .39 .08 .03 5.4 7.3 4.2 .7 .0 10 10 10 10 10 10 10 10 10 10 10 1536 Appendix 5 Individual Clinical Hematology Data Males Week 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ANISO POLY POIK HYPO TOXNEUT Group: 1 C92508 C92509 C92511 C92521 C92527 C92528 C92532 C92535 C92537 C92562 Group: 2 C92583 C92593 C92598 C92600 C92612 C92616 C92621 C92624 C92627 C92629 Dose Level: 0 -------------------- Dose Level: 0.5 --------------------- Dosage Unit: ppm T-6295 - - Dosage Unit: ppm T-6295 ----------- Covance 6329-183 3M T-6295 1537 Appendix 5 Individual Clinical Hematology Data Males Week 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ANISO POLY POIK HYPO TOXNEUT Group: 3 C92636 C92640 C92644 C92645 C92651 C92661 C92662 C92676 C92682 C92684 Group: 4 C92711 C92713 C92714 C92716 C92719 C92722 C92726 C92728 C92741 C92748 Dose Level: 2.0 -------------------- Dose Level: 5.0 --------------------- Dosage Unit: ppm T-6295 - - Dosage Unit: ppm T-6295 ----------- Covance 6329-183 3M T-6295 1538 Appendix 5 Individual Clinical Hematology Data Males Week 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ANISO POLY POIK HYPO TOXNEUT Group: 5 C92754 C92765 C92767 C92774 C92777 C92784 C92789 C92799 C92812 C92820 Dose Level: 20.0 --------------------- Dosage Unit: ppm T-6295 ----------- Covance 6329-183 3M T-6295 1539 Appendix 5 Individual Clinical Hematology Data Females Week 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL RBC HGB HCT MCV MCH MCHC PLT NUMBER X 1 0 6/^L G/DL % FL PG % X 103/m L Group: 1 C92863 C92865 C92871 C92880 C92887 C92898 C92903 C92905 C92906 C92927 MEAN S.D. N Group: 2 C92935 C92940 C92944 C92948 C92956 C92962 C92967 C92978 C92986 C92987 MEAN S.D. N Dose Level: 0 8.49 8. 00 8.90 8.61 7.73 7.65 8.13 7.78 7.55 7.17 8. 00 .535 10 15.9 15.5 16.8 16.1 14.9 14.7 15.7 15.0 14.4 13.9 15.3 .87 10 Dose Level: 0.5 8.37 8.65 8.46 7.60 7.90 7.90 7.89 8.24 8.40 8.75 15.0 16.2 15.8 15.0 14.5 16.0 14.9 15.9 15.9 15.8 8. 22 .377 10 15.5 .59 10 Dosage Unit: ppm T-6295 47.9 45.8 50.3 46.8 43.1 42.9 46.0 43.9 43.5 42.2 56.4 57.3 56.5 54.4 55.7 56.0 56.6 56.4 57.7 58.8 18.8 19.4 18.9 18.7 19.2 19.2 19.4 19.2 19.1 19.4 45.2 2.58 10 56.6 1.18 10 19.1 .25 10 Dosage Unit: ppm T-6295 45.8 49.6 46.6 45.3 43.9 45.2 43.3 46.5 47.6 45.8 54.7 57.4 55.1 59.6 55.6 57.1 54.9 56.4 56.6 52.4 17.9 18.7 18.7 19.8 18.3 20.2 18.9 19.3 18.9 18.0 46.0 1.80 10 56.0 1.93 10 18.9 .74 10 33.2 33.9 33.5 34.4 34.5 34.3 34.2 34.1 33.1 33.0 33.8 .57 10 32.7 32.6 33.9 33.2 32.9 35.4 34.4 34.2 33.3 34.4 33.7 .91 10 1041 1022 1194 1054 1078 1094 1133 1027 1015 948 1061 68 10 1153 1091 1234 1136 1126 1092 1206 1009 1207 1179 1143 67 10 Covance 6329-183 3M T-6295 1540 Appendix 5 Individual Clinical Hematology Data Females Week 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL RBC HGB HCT MCV MCH MCHC PLT NUMBER X106///L G/DL FL PG X103///L Group: 3 C92992 C92993 C93000 C93018 C93019 C93023 C93024 C93026 C93030 C93035 MEAN S.D. N Group: 4 C93051 C93064 C93071 C93076 C93078 C93085 C93089 C93090 C93093 C93109 MEAN S.D. N Dose Level: 2. 0 7.82 7.80 8.31 7.95 8.06 8.38 8.34 7.87 7.76 8.32 15.3 14.5 15.2 15.5 14.8 15.1 16.5 14.5 15.4 15.3 8.06 .253 10 15.2 .58 10 Dose Level: 5.0 8.40 8.55 7.84 7.30 8.09 7.49 7.81 7.51 8.15 8.56 16.0 16.1 15.0 14.5 15.2 14.6 14.6 14.3 15.8 15.9 7.97 .453 10 15.2 .70 10 Dosage Unit: ppm T-6295 46.1 42.2 44.5 45.8 45.2 44.8 49.1 44.2 45.8 44.5 58.9 54.1 53.5 57.6 56.1 53.5 58.8 56.2 59.1 53.5 19.6 18.6 18.2 19.4 18.3 18.0 19.8 18.4 19.8 18.3 45.2 1.76 10 56.1 2.37 10 18.8 .72 10 Dosage Unit: ppm T-6295 46.8 47.0 44.0 42.9 46.5 41.7 44.1 42.5 47.4 46.9 55.7 55.0 56.1 58.8 57.5 55.7 56.5 56.6 58.1 54.7 19.0 18.8 19.2 19.8 18.8 19.5 18.6 19.0 19.3 18.5 45.0 2.17 10 56.5 1.32 10 19.0 .41 10 33.3 34.4 34.1 33.8 32.7 33.7 33.6 32.8 33.6 34.3 33.6 .57 10 34.2 34.1 34.1 33.7 32.7 34.9 33.0 33.6 33.2 33.9 33.7 .65 10 993 989 1126 1118 1070 1201 1056 1192 902 1058 1070 93. 10 1174 1120 1055 1088 1083 1039 1181 1025 972 1078 1082 64 10 Covance 6329-183 3M T-6295 1541 Appendix 5 Individual Clinical Hematology Data Females Week 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL RBC HGB HCT MCV MCH MCHC PLT NUMBER X106//2L G/DL FL PG X103//2L Group: 5 C93111 C93113 C93120 C93127 C93135 C93143 C93155 C93162 C93169 C93178 MEAN S.D. N Dose Level: 20.0 7.78 8. 22 7.59 7.90 8.08 8.10 7.89 7.44 8.04 7.70 14.7 15.3 14.3 15.5 15.1 16.1 15.7 13.8 15.1 15.3 7.87 .247 10 15.1 .67 10 Dosage Unit: ppm T-6295 41.0 46.4 43.0 44.2 45.6 46.3 44.4 41.9 43.8 45.4 52.7 56.4 56.7 56.0 56.5 57.1 56.3 56.3 54.5 59.0 18.9 18.6 18.8 19.6 18.7 19.9 19.9 18.5 18.8 19.9 44.2 1.82 10 56.2 1.64 10 19.2 .59 10 35.9 33.1 33.2 35.0 33.1 34.8 35.3 32.9 34.4 33.7 34.1 1.08 10 1033 1128 1188 1150 1024 1226 1180 1339 1250 921 1144 122.9 10 Covance 6329-183 3M T-6295 1542 ANIMAL NUMBER Group: 1 C92863 C92865 C92871 C92880 C92887 C92898 C92903 C92905 C92906 C92927 MEAN S.D. N Group: 2 C92935 C92940 C92944 C92948 C92956 C92962 C92967 C92978 C92986 C92987 MEAN S.D. N Appendix 5 Individual Clinical Hematology Data Females Week 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS WBC X103///L N-SEG LYMPH X103///L X103//L MONO EOSIN X 103//L X103//L BASO N-SEG% X 103//L LYMPH% MONO% EOSIN% Covance 6329-183 3M T-6295 BASO% Dose Level : 0 Dosage Unit: ppm T-6295 4.5 .7 3.0 .6 .2 .0 15 67 14 4 0 6.4 2 . 1 3.4 .7 . 1 .0 33 54 11 2 0 6.5 1.5 4.4 .4 . 1 .0 24 68 7 1 0 2.3 .8 1.3 . 1 .0 .0 34 58 5 2 0 3.5 .9 2 . 1 .4 . 1 .0 25 60 12 2 0 6. 6 1.3 4.6 .5 .2 .0 20 70 7 3 0 4.4 .7 3.3 .3 . 1 .0 17 75 6 3 0 3.7 .3 3.1 .3 .0 .0 9 83 7 1 0 6.4 .7 5.1 .4 . 1 .0 11 80 7 2 0 4.6 .6 3.5 .4 . 1 .0 13 77 8 2 0 4.9 1. 0 3.4 .4 . 1 .0 20 69 8 2 0 1.51 .53 1.14 .17 .07 .00 8.8 9.7 2 ..9 .9 .0 10 10 10 10 10 10 10 10 10 10 10 Dose Level: 0.5 Dosage Unit: ppm T-6295 4.4 .8 3.0 .5 . 1 .0 18 68 12 2 0 9.6 1.7 7.0 .7 . 1 .0 18 73 7 1 0 9.0 1.4 7.0 .5 . 1 .0 15 78 6 1 0 10. 2 1 . 8 7.2 1. 0 . 1 .0 18 71 9 1 0 3.1 .9 1 . 6 .5 . 1 .0 28 53 16 3 0 4.5 .5 3.6 .2 . 1 .0 12 81 5 2 0 4.0 .7 3.0 .2 .0 .0 17 76 6 1 0 6 . 1 1. 0 4.5 .6 . 1 .0 16 73 9 2 0 5.0 .7 3.5 .7 . 1 .0 15 69 13 3 0 8. 6 .7 7.5 .3 . 1 .0 8 87 3 1 0 6.4 1. 0 4.8 .5 . 1 .0 16 73 9 2 0 2.64 .45 2.18 .25 .03 .00 5.1 9.1 4.0 .8 .0 10 10 10 10 10 10 10 10 10 10 10 1543 ANIMAL NUMBER Group: 3 C92992 C92993 C93000 C93018 C93019 C93023 C93024 C93026 C93030 C93035 MEAN S.D. N Group: 4 C93051 C93064 C93071 C93076 C93078 C93085 C93089 C93090 C93093 C93109 MEAN S.D. N Appendix 5 Individual Clinical Hematology Data Females Week 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS WBC X103///L N-SEG LYMPH X103///L X103//L MONO EOSIN X 103//L X103//L BASO N-SEG% X103/iL LYMPH% MONO% EOSIN% Covance 6329-183 3M T-6295 BASO% Dose Level : 2. 0 Dosage Unit: ppm T-6295 4.1 .9 2.9 .3 . 1 .0 21 70 7 2 0 3.8 .4 3.1 .3 .0 .0 11 80 7 1 0 3.8 .7 2.7 .3 . 1 .0 18 71 8 2 0 6.4 .7 5.3 .3 . 1 .0 10 83 5 2 0 3.8 .6 2.9 .3 . 1 .0 15 75 8 3 0 3.0 .5 2. 2 .2 . 1 .0 18 74 5 3 0 4.5 .8 3.4 .3 . 1 .0 17 75 6 1 0 7.7 .9 6.3 .4 . 1 .0 12 81 5 1 0 4.8 .6 3.8 .3 . 1 .0 12 79 7 3 0 7.4 .5 6. 2 .5 . 1 .0 7 85 7 1 0 4.9 .7 3.9 .3 . 1 .0 14 77 6 2 0 1.65 .17 1.50 .08 .03 .00 4.4 5.1 1 . 2 .9 .0 10 10 10 10 10 10 10 10 10 10 10 Dose Level: 5.0 Dosage Unit: ppm T-6295 6.0 .6 5.2 .2 . 1 .0 10 85 3 1 0 3.2 .6 2 . 1 .3 .0 .0 20 68 11 1 1 5.8 .4 5.1 .2 . 1 .0 7 88 4 1 0 6.5 .8 5.1 .5 .2 .0 12 78 7 3 0 5.1 1 . 2 3.3 .4 . 1 .0 24 65 8 2 0 4.4 .5 3.5 .3 . 1 .0 10 80 7 2 0 8.3 .5 6. 2 1.3 . 1 . 1 6 75 16 1 1 9.6 2. 2 6.8 .4 . 1 .0 23 71 4 1 0 5.8 1.3 4.1 .3 . 1 .0 23 69 6 2 0 3.8 .7 2. 6 .3 . 1 .0 19 69 9 3 0 5.8 .9 4.4 .4 . 1 .0 15 75 8 2 0 1.96 .55 1.54 .32 .05 .03 7.1 7.7 3.9 .8 .4 10 10 10 10 10 10 10 10 10 10 10 1544 ANIMAL NUMBER Appendix 5 Individual Clinical Hematology Data Females Week 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS WBC X103///L N-SEG LYMPH X103///L X103//L MONO EOSIN X103//L X103//L BASO N-SEG% X103//L LYMPH% MONO% EOSIN% Covance 6329-183 3M T-6295 BASO% Group: 5 Dose Level : 20.0 Dosage Unit: ppm T-6295 C93111 C93113 C93120 C93127 C93135 C93143 C93155 C93162 C93169 C93178 4.8 7.2 1..08 3.7 5.5 5.4 3.1 5.7 3.2 ..67 1.1 .7 42..20 42..01 3.2 .5 2.5 7.6 3.4 4.0 2..67 .8 4.4 2.7 3.0 ....6621 .4 ..23 ..24 .3 ..........2001111111 ..........0000000000 17 15 13 18 19 23 15 35 16 19 78 76 73 72 71 62 76 58 78 74 1238 78 10 6 6 5 7 231 23 5 31 1 1 1000000000 MEAN S.D. 41..688 1.0 .64 31..142 .3 .17 .1 .06 ..000 19 6.3 72 6.7 7 2.5 2 1.3 0 .3 N 10 10 10 10 10 10 10 10 10 10 10 1545 Appendix 5 Individual Clinical Hematology Data Females Week 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ANISO POLY POIK HYPO TOXNEUT Group: 1 C92863 C92865 C92871 C92880 C92887 C92898 C92903 C92905 C92906 C92927 Group: 2 C92935 C92940 C92944 C92948 C92956 C92962 C92967 C92978 C92986 C92987 Dose Level: 0 -------------------- Dose Level: 0.5 --------------------- Dosage Unit: ppm T-6295 - - Dosage Unit: ppm T-6295 ----------- Covance 6329-183 3M T-6295 1546 Appendix 5 Individual Clinical Hematology Data Females Week 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ANISO POLY POIK HYPO TOXNEUT Group: 3 C92992 C92993 C93000 C93018 C93019 C93023 C93024 C93026 C93030 C93035 Group: 4 C93051 C93064 C93071 C93076 C93078 C93085 C93089 C93090 C93093 C93109 Dose Level: 2.0 --------------------- Dose Level: 5.0 Dosage Unit: ppm T-6295 ----------- Dosage Unit: ppm T-6295 Covance 6329-183 3M T-6295 1547 Appendix 5 Individual Clinical Hematology Data Females Week 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ANISO POLY POIK HYPO TOXNEUT Group: 5 C93111 C93113 C93120 C93127 C93135 C93143 C93155 C93162 C93169 C93178 Dose Level: 20.0 --------------------- Dosage Unit: ppm T-6295 ----------- Covance 6329-183 3M T-6295 1548 Appendix 5 Individual Clinical Hematology Data Males Week 27 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL RBC HGB HCT MCV MCH MCHC PLT NUMBER X 1 0 6/^L G/DL % FL PG % X 103/m L Group: 1 C92505 C92510 C92512 C92525 C92537 C92543 C92545 C92563 C92567 C92570 MEAN S.D. N Group: 2 C92577 C92583 C92585 C92586 C92588 C92590 C92601 C92602 C92617 C92630 MEAN S.D. N Dose Level: 0 8.48 8.86 8.77 8.82 8.32 8.18 8.90 8.51 9.20 9.81 15.6 15.8 16.3 15.2 15.0 14.8 15.6 15.8 16.2 16.6 8.78 .471 10 15.7 .58 10 Dose Level: 0.5 9.24 8.48 7.82 9.59 8.58 8.83 8.11 9.84 9.11 8.83 16.2 15.2 13.7 15.7 15.3 15.2 15.0 16.8 15.8 16.1 8.84 .629 10 15.5 .84 10 Dosage Unit: ppm T-6295 46.3 46.0 48.4 45.3 43.7 42.8 45.8 46.2 47.8 49.1 54.5 52.0 55.2 51.4 52.5 52.3 51.5 54.3 52.0 50.1 18.3 17.8 18.6 17.3 18.1 18.0 17.5 18.6 17.6 16.9 46.1 1.96 10 52.6 1.60 10 17.9 .56 10 Dosage Unit: ppm T-6295 48.0 44.8 41.3 47.2 45.7 44.9 43.9 49.4 46.8 47.6 52.0 52.8 52.9 49.2 53.3 50.8 54.1 50.3 51.3 53.9 17.6 18.0 17.6 16.4 17.8 17.2 18.5 17.1 17.4 18.2 46.0 2.34 10 52.1 1.63 10 17.6 .60 10 33.6 34.3 33.6 33.6 34.4 34.4 34.0 34.3 33.8 33.8 34.0 .34 10 33.8 34.0 33.2 33.3 33.4 33.7 34.1 34.0 33.8 33.7 33.7 .31 10 1112 1185 965 1414 1325 1130 1040 1318 1464 1394 1235 171 10 1197 1249 1262 1294 1483 1291 1003 1139 1360 1424 1270 138 10 Covance 6329-183 3M T-6295 1549 Appendix 5 Individual Clinical Hematology Data Males Week 27 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL RBC HGB HCT MCV MCH MCHC PLT NUMBER X 106/m L G/DL % FL PG % X 103/m L Group: 3 C92633 C92644 C92647 C92658 C92660 C92667 C92673 C92679 C92686 C92687 MEAN S.D. N Group: 4 C92696 C92698 C92708 C92709 C92710 C92725 C92743 C92745 C92749 C92750 MEAN S.D. N Dose Level: 2.0 8.78 8.46 8.57 8.56 9.53 8.87 8.96 8.69 8.82 8.92 15.9 14.9 14.8 14.8 16.2 15.7 16.0 15.7 15.7 15.0 8.82 .301 10 15.5 .54 10 Dose Level: 5.0 8.70 9.46 10.00 9.10 8.80 8.63 9.08 9.19 8.53 8.95 15.8 16.6 16.4 16.1 15.1 15.4 16.1 15.4 14.7 16.1 9.04 .439 10 15.8 .60 10 Dosage Unit: ppm T-6295 46.2 44.2 43.4 43.9 48.5 46.7 47.2 45.9 45.7 44.5 52.6 52.2 50.7 51.3 50.9 52.7 52.6 52.8 51.8 49.9 18.1 17.6 17.3 17.2 17.0 17.8 17.8 18.1 17.8 16.8 45.6 1.62 10 51.8 1.01 10 17.5 .45 10 Dosage Unit: ppm T-6295 46.1 48.4 49.3 47.5 44.4 45.6 47.2 46.3 43.4 48.1 53.0 51.2 49.2 52.2 50.5 52.8 52.0 50.3 50.9 53.7 18.1 17.5 16.3 17.7 17.1 17.9 17.7 16.7 17.2 17.9 46.6 1.84 10 51.6 1.40 10 17.4 .58 10 34.5 33.8 34.1 33.6 33.4 33.7 33.8 34.3 34.4 33.6 33.9 .38 10 34.2 34.2 33.2 33.9 33.9 33.8 34.1 33.3 33.8 33.4 33.8 .36 10 1325 1313 1003 1112 1219 1243 1324 1384 1097 1354 1237 127 10 1227 1289 1300 1176 1397 1072 1303 1074 1062 1269 1217 116 10 Covance 6329-183 3M T-6295 1550 Appendix 5 Individual Clinical Hematology Data Males Week 27 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL RBC HGB HCT MCV MCH MCHC PLT NUMBER X106///L G/DL FL PG X103///L Group: 5 C92756 C92761 C92764 C92773 C92783 C92788 C92791 C92803 C92804 C92820 MEAN S.D. N Dose Level: 20.0 8.64 8.64 8.71 9.27 8.59 8.28 8.75 8.46 8.70 8.66 14.8 16.4 15.2 15.2 14.6 14.9 14.6 14.8 14.6 16.0 8.67 .252 10 15.1 .62 10 Dosage Unit: ppm T-6295 44.6 47.6 44.5 44.7 44.0 44.6 43.2 44.5 43.9 45.9 51.6 55.1 51.1 48.2 51.3 53.8 49.4 52.6 50.4 53.0 17.2 19.0 17.4 16.4 17.0 18.0 16.7 17.5 16.8 18.5 44.7 1.22 10 51.6 2.06 10 17.4 .83 10 33.3 34.5 34.1 34.0 33.2 33.4 33.8 33.3 33.3 34.8 33.8 .57 10 1362 1349 1367 1342 1068 1021 1394 1473 1087 1410 1287 162.9 10 Covance 6329-183 3M T-6295 1551 ANIMAL NUMBER Group: 1 C92505 C92510 C92512 C92525 C92537 C92543 C92545 C92563 C92567 C92570 MEAN S.D. N Group: 2 C92577 C92583 C92585 C92586 C92588 C92590 C92601 C92602 C92617 C92630 MEAN S.D. N Appendix 5 Individual Clinical Hematology Data Males Week 27 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS WBC X103//2L N-SEG LYMPH X103//2L X103//2L MONO EOSIN X103//2L X103//2L BASO N-SEG% X103//2L LYMPH% MONO% EOSIN% Covance 6329-183 3M T-6295 BASO% Dose Level : 0 Dosage Unit: ppm T-6295 8.0 1 . 2 5.6 1. 0 .1 .1 16 70 13 1 1 8.9 1 . 2 6.0 1.3 .3 .0 14 68 15 3 0 7.4 1.7 4.6 1. 0 .1 .1 23 62 14 1 1 10.6 1 . 6 7.6 1 . 2 .2 .1 15 72 11 2 1 7.6 .8 5.5 1 . 1 .2 .1 10 72 14 2 1 5.8 1 . 2 3.9 .5 .1 .0 21 68 9 2 0 6.4 1 . 1 4.3 .9 .1 .0 17 67 14 1 1 6.7 1 . 2 4.3 1 . 1 .1 .1 17 64 16 2 1 7.6 1.7 4.7 1. 0 .3 .0 22 61 13 4 0 6.2 1.3 4.0 .7 .2 .0 21 64 11 3 1 7.5 1.3 5.0 1. 0 .2 .0 18 67 13 2 1 1.43 .29 1.14 .23 .08 .05 4.1 3.9 2 . 1 1. 0 .5 10 10 10 10 10 10 10 10 10 10 10 Dose Level: 0.5 Dosage Unit: ppm T-6295 8.5 1.4 6.2 .7 .2 .0 16 72 8 3 0 7.6 1 . 6 4.6 1 . 1 .2 .1 22 61 15 2 1 6.5 1.4 4.2 .7 .2 .0 22 64 10 3 1 7.5 1.9 4.6 .8 .2 .0 25 61 11 3 1 8.7 1.4 6.4 .7 .2 .0 16 73 8 3 0 8.4 1 . 1 6 . 1 1. 0 .2 .0 13 73 12 2 1 11.5 1.7 8.4 1 . 1 .2 .1 15 73 10 1 1 6.7 2.0 3.8 .7 .2 .0 29 57 10 3 0 7.8 1.3 5.2 1 . 1 .2 .0 16 67 14 3 1 9.1 1 . 1 6.7 1 . 1 .1 .1 12 73 12 2 1 8.2 1.5 5.6 .9 .2 .0 19 67 11 2 1 1.42 .31 1.40 .19 .03 .05 5.6 6.2 2.3 .7 .5 10 10 10 10 10 10 10 10 10 10 10 1552 ANIMAL NUMBER Group: 3 C92633 C92644 C92647 C92658 C92660 C92667 C92673 C92679 C92686 C92687 MEAN S.D. N Group: 4 C92696 C92698 C92708 C92709 C92710 C92725 C92743 C92745 C92749 C92750 MEAN S.D. N Appendix 5 Individual Clinical Hematology Data Males Week 27 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS WBC X103///L N-SEG LYMPH X103///L X103//L MONO EOSIN X103///L X103//L BASO N -SEG% X103///L LYMPH% MONO% EOSIN% Covance 6329-183 3M T-6295 BASO% Dose Level : 2.0 Dosage Unit: ppm 'T-6295 7.8 1 . 8 4.9 .9 .2 .0 22 64 11 2 0 5.8 1 . 2 3.8 .7 .1 .0 20 66 11 1 1 8.3 1 . 1 6.2 .8 .2 .0 13 75 9 2 0 9.4 2.4 5.5 1 . 2 .2 .0 26 58 13 3 0 7.6 1 . 1 5.5 .7 .1 .1 15 73 9 2 1 9.4 1.3 6.9 1. 0 .2 .0 14 73 10 2 0 7.4 2.4 3.7 1 . 2 .2 .0 32 50 16 2 0 10.2 1.5 7.4 1 . 1 .2 .0 14 72 11 2 0 10.6 2.2 6.8 1.2 .3 .0 21 64 12 3 0 1 0 . 1 1.7 6.5 1.5 .3 .1 17 65 14 3 1 8.7 1.7 5.7 1. 0 .2 .0 19 66 12 2 0 1.53 .52 1.28 .26 .07 .04 6 ..1 7..8 2 ..2 .6 .5 10 10 10 10 10 10 10 10 10 10 10 Dose Level : 5.0 Dosage Unit: ppm 'T-6295 7.6 1 . 2 5.6 .6 .2 .0 15 74 8 2 0 8.8 1.7 5.5 1.3 .2 .1 19 62 15 2 1 1 0 . 1 1.7 6.3 1 . 8 .2 .0 17 62 18 2 0 6.3 1.5 3.7 .9 .2 .0 24 59 14 2 0 9.1 2.0 5.7 1 . 2 .1 .0 23 62 13 2 0 9.6 2.0 6.3 1 . 1 .2 .1 20 66 12 2 1 12.5 1 . 1 9.9 1 . 2 .2 .1 9 79 10 1 1 8.8 1.9 5.3 1.3 .2 .1 21 61 15 2 1 6.5 1.4 4.1 .9 .1 .0 22 63 13 1 1 7.1 1 . 6 4.2 1 . 2 .1 .1 22 58 17 2 1 8.6 1 . 6 5.7 1 . 2 .2 .0 19 65 14 2 1 1.88 .31 1.74 .32 .05 .05 4..5 6 .7 3..0 .4 .5 10 10 10 10 10 10 10 10 10 10 10 1553 ANIMAL NUMBER Group: 5 C92756 C92761 C92764 C92773 C92783 C92788 C92791 C92803 C92804 C92820 MEAN S.D. N Appendix 5 Individual Clinical Hematology Data Males Week 27 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS WBC X103///L N-SEG LYMPH X103///L X103//L MONO EOSIN X 103//L X103//L BASO N-SEG% X103///L LYMPH% MONO% EOSIN% Covance 6329-183 3M T-6295 BASO% Dose Level : 20.0 Dosage Unit: ppm T-6295 6.4 1. 0 4.4 .9 .1 .0 15 69 14 1 0 1 2. 2 2 . 1 8.4 1.5 .1 .1 17 69 12 1 1 7.0 1 . 6 4.3 .9 .2 .0 22 61 14 2 1 7.0 1 . 2 5.0 .6 .2 .0 18 71 8 3 1 8.5 1 . 8 5.2 1.3 .1 .0 22 61 15 2 0 9.9 2. 2 6.3 1 . 2 .1 .0 22 64 12 1 0 10.3 1 . 8 7.3 1. 0 .1 .1 18 71 10 1 1 6.8 1 . 1 4.7 .8 .2 .0 16 70 12 2 0 9.6 1.4 6.9 1 . 1 .2 .0 14 72 12 2 0 9.0 1.3 6.9 .7 .1 .0 14 76 7 1 0 8.7 1 . 6 5.9 1. 0 .1 .0 18 68 12 2 0 1.88 .42 1.41 .28 .05 .04 3.2 4.9 2.6 .7 .5 10 10 10 10 10 10 10 10 10 10 10 1554 Appendix 5 Individual Clinical Hematology Data Males Week 27 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ANISO POLY POIK HYPO TOXNEUT Group: 1 C92505 C92510 C92512 C92525 C92537 C92543 C92545 C92563 C92567 C92570 Group: 2 C92577 C92583 C92585 C92586 C92588 C92590 C92601 C92602 C92617 C92630 Dose Level: 0 -------------------- Dose Level: 0.5 --------------------- Dosage Unit: ppm T-6295 - - Dosage Unit: ppm T-6295 ----------- Covance 6329-183 3M T-6295 1555 Appendix 5 Individual Clinical Hematology Data Males Week 27 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ANISO POLY POIK HYPO TOXNEUT Group: 3 C92633 C92644 C92647 C92658 C92660 C92667 C92673 C92679 C92686 C92687 Group: 4 C92696 C92698 C92708 C92709 C92710 C92725 C92743 C92745 C92749 C92750 Dose Level: 2.0 --------------------- Dose Level: 5.0 ----------- Dosage Unit: ppm T-6295 ----------- Dosage Unit: ppm T-6295 Covance 6329-183 3M T-6295 1556 Appendix 5 Individual Clinical Hematology Data Males Week 27 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ANISO POLY POIK HYPO TOXNEUT Group: 5 C92756 C92761 C92764 C92773 C92783 C92788 C92791 C92803 C92804 C92820 Dose Level: 20.0 --------------------- Dosage Unit: ppm T-6295 ----------- Covance 6329-183 3M T-6295 1557 Appendix 5 Individual Clinical Hematology Data Females Week 27 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL RBC HGB HCT MCV MCH MCHC PLT NUMBER X106/^L G/DL % FL PG % X 103/m L Group: 1 C92876 C92878 C92888 C92906 C92916 C92917 C92921 C92922 C92923 C92928 MEAN S.D. N Group: 2 C92932 C92935 C92938 C92951 C92954 C92956 C92960 C92974 C92979 C92989 MEAN S.D. N Dose Level: 0 7.70 7.02 8.77 6.91 7.04 7.40 7.66 7.28 7.77 8.04 15.0 13.7 16.3 13.3 13.6 14.4 14.6 13.8 15.3 15.5 7.56 .563 10 14.6 .97 10 Dose Level: 0.5 6.36 8.39 8.07 7.82 7.35 8.16 8.28 8.47 8.30 8.08 14.0 15.3 15.0 14.7 14.1 15.0 15.7 15.0 15.1 15.2 7.93 .639 10 14.9 .52 10 Dosage Unit: ppm T-6295 45.1 40.6 49.8 39.9 41.4 41.2 44.2 41.2 45.8 45.9 58.6 57.8 56.8 57.8 58.8 55.6 57.7 56.6 58.9 57.1 19.5 19.5 18.6 19.2 19.3 19.5 19.1 18.9 19.7 19.2 43.5 3.16 10 57.6 1.06 10 19.2 .33 10 Dosage Unit: ppm T-6295 40.9 46.1 45.1 43.9 42.4 45.7 47.4 45.2 45.5 46.0 64.3 54.9 55.9 56.2 57.7 56.0 57.2 53.4 54.9 57.0 22.0 18.2 18.6 18.8 19.2 18.4 18.9 17.7 18.2 18.9 44.8 1.92 10 56.8 2.94 10 18.9 1.18 10 33.3 33.8 32.8 33.2 32.8 35.0 33.1 33.4 33.4 33.6 33.4 .63 10 34.2 33.2 33.3 33.4 33.3 32.9 33.1 33.2 33.2 33.1 33.3 .35 10 1168 1150 1102 841 1043 1152 1057 828 1102 1071 1051 121 10 797 1158 1116 1085 1001 1205 930 1025 1201 1049 1057 127 10 Covance 6329-183 3M T-6295 1558 Appendix 5 Individual Clinical Hematology Data Females Week 27 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL RBC HGB HCT MCV MCH MCHC PLT NUMBER X 106/m L G/DL % FL PG % X 103/m L Group: 3 C93003 C93004 C93011 C93020 C93027 C93030 C93034 C93046 C93047 C93049 MEAN S.D. N Group: 4 C93052 C93055 C93072 C93080 C93081 C93090 C93094 C93095 C93103 C93104 MEAN S.D. N Dose Level: 2.0 7.70 8.19 8.15 7.79 8.59 7.85 7.49 8.22 7.96 8.03 8.00 .312 10 14.5 15.2 15.7 14.4 16.2 15.7 14.4 15.4 14.4 15.0 15.1 .66 10 Dose Level: 5.0 8.61 8.04 8.38 7.74 8.40 7.84 8.61 7.69 7.86 8.00 15.7 15.9 16.1 14.3 15.6 14.4 15.7 14.9 15.4 15.4 8.12 .353 10 15.3 .61 10 Dosage Unit: ppm T-6295 43.6 45.5 46.8 43.3 48.9 46.4 43.2 45.7 42.9 45.9 56.6 55.6 57.4 55.6 56.9 59.1 57.7 55.6 53.9 57.1 18.8 18.5 19.2 18.5 18.8 20.0 19.3 18.8 18.1 18.7 45.2 1.94 10 56.5 1.44 10 18.9 .53 10 Dosage Unit: ppm T-6295 47.2 46.4 47.9 43.4 46.9 43.9 48.1 44.8 45.6 46.5 54.9 57.7 57.1 56.1 55.8 56.0 55.8 58.3 57.9 58.2 18.2 19.7 19.1 18.5 18.6 18.4 18.3 19.4 19.6 19.3 46.1 1.61 10 56.8 1.20 10 18.9 .57 10 33.2 33.3 33.5 33.3 33.1 33.8 33.4 33.7 33.6 32.7 33.4 .32 10 33.2 34.2 33.5 33.0 33.3 32.9 32.7 33.3 33.8 33.1 33.3 .44 10 934 1154 1080 1123 1119 979 1070 1099 1163 1023 1074 75 10 1098 1065 1358 1056 1240 1092 1160 925 1013 944 1095 131 10 Covance 6329-183 3M T-6295 1559 Appendix 5 Individual Clinical Hematology Data Females Week 27 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL RBC HGB HCT MCV MCH MCHC PLT NUMBER X106/iL G/DL % FL PG % X103/iL Group: 5 C93119 C93131 C93133 C93138 C93149 C93153 C93157 C93160 C93162 C93179 MEAN S.D. N Dose Level: 20.0 7.83 7.52 7.46 8.05 8.24 7.96 8.04 6.97 7.31 7.91 14.8 13.9 14.6 14.3 15.1 14.6 14.9 13.3 14.6 14.7 7.73 .398 10 14.5 .53 10 Dosage Unit: ppm T-6295 44.2 41.6 43.2 43.0 46.1 44.0 43.3 39.7 43.7 44.1 56.5 55.4 57.9 53.4 55.9 55.3 53.8 56.9 59.8 55.7 19.0 18.5 19.6 17.7 18.4 18.4 18.5 2190..01 18.6 43.3 1.70 10 56.1 1.87 10 18..686 10 33.6 33.3 33.9 33.2 32.9 33.3 34.3 33.6 33.5 33.3 33.5 .39 10 1040 1065 867 907 1229 1075 1081 848 1278 1233 1062 153.7 10 Covance 6329-183 3M T-6295 1560 ANIMAL NUMBER Group: 1 C92876 C92878 C92888 C92906 C92916 C92917 C92921 C92922 C92923 C92928 MEAN S.D. N Group: 2 C92932 C92935 C92938 C92951 C92954 C92956 C92960 C92974 C92979 C92989 MEAN S.D. N Appendix 5 Individual Clinical Hematology Data Females Week 27 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS WBC X103///L N-SEG LYMPH X103///L X103//L MONO EOSIN X103///L X103//L BASO N-SEG% X 103//L LYMPH% MONO% EOSIN% Covance 6329-183 3M T-6295 BASO% Dose Level : 0 Dosage Unit: ppm T-6295 5.2 1 . 1 3.4 .5 . 1 .0 22 66 10 2 0 5.5 2. 0 3.0 .5 . 1 .0 36 53 9 2 0 6.0 .4 4.8 .7 . 1 .0 7 79 11 2 0 5.1 .9 3.8 .3 . 1 .0 17 74 7 2 1 6.9 .9 5.1 .5 .4 .0 12 74 7 6 0 5.0 .4 3.8 .6 . 1 .0 9 75 13 2 0 5.0 .6 3.8 .5 .2 .0 12 75 10 3 0 5.1 .3 4.4 .3 . 1 .0 6 87 6 2 0 6.8 1.5 4.0 1 . 2 . 1 . 1 22 59 17 1 1 4.8 .7 3.7 .4 . 1 .0 14 76 8 2 0 5.5 .9 4.0 .6 . 1 .0 16 72 10 2 0 .77 .54 .63 .26 .10 .03 9.1 9.9 3.3 1.3 .4 10 10 10 10 10 10 10 10 10 10 10 Dose Level: 0.5 Dosage Unit: ppm T-6295 19.3 4.4 13.2 1.3 .2 .1 23 69 7 1 0 3.3 .4 2.4 .4 . 1 .0 11 74 13 2 0 6. 2 .4 5.4 .4 . 1 .0 6 87 6 1 0 2. 8 .6 2 . 1 .1 .1 .0 21 73 4 2 0 7.6 1 . 2 5.8 .5 .2 .0 15 76 7 2 0 3.2 .4 2. 6 .2 . 1 .0 11 82 5 2 0 5.8 .8 4.5 .5 . 1 .0 13 77 8 2 0 5.0 .5 4.1 .4 . 1 .0 9 81 8 1 0 5.1 .8 3.6 .6 . 1 .0 15 70 12 3 1 8. 6 1.5 6. 6 .3 .2 .0 18 77 3 2 0 6.7 1 . 1 5.0 .5 . 1 .0 14 77 7 2 0 4.82 1.22 3.24 .33 .05 .03 5.3 5.6 3.2 .6 .3 10 10 10 10 10 10 10 10 10 10 10 1561 ro in co cn HI u()N O I m E-i ro 2 ro d) Ut rd > o u Individual Clinical Hematology Data oooooooooo oo\ w C CQ H H C N H -s^ rO C N C N C N *^ o\ s o w 'X '^ ^ U J'X io L O c o O iro oo\ so2 OOOHOOOOHO (N H rO H H C N C N C N C N C N oco'X'(NLn^'X''X'i>i> off\i p-l 2X P o\ o H P C/3O <|H CQ X oooooooooo oooooooooo rlrlrlrlO ]H rlrlrlrl 3- iCO d) i-- o 5 iit! os 8P 3^ 1w-1 5M ^ 1 in o U i^ 1 i> fc ifdcs) K, U l cc Wx ; n Eh Fh U g ffttX Q O8 *! O 1 4P CO r o r o in r o u^ -H 1 d P Og nu p ^P c> p1 fp-fli \ 3 . 11 2 i JH X d) Di rd CO 0 Q r o !> o in i> o in CO r o o L4) r o r o r o ro rH CO rH O rH [> i> O O rH ro (NrHCNrHrHCNrHrHrHrH in o i> Fh Ffftti l>^ro'>ro^ro(N''0 4P -H d P d) U) (6 coHHco'>'>[>'>ro'> l> i> o CO rH n in^^ro^corocN'X'ro st1 rH U ji rd 3W i i Ul o 1 O o rH rH 5X | i D a. ! 01-o 1 !s O rH 1 1 X1 i-- 1 d) > d) P d) CO 0 O !> i>in OOOOLDfO'X'OOO CO H o rH *nF rH <D > d) i>-st<(N(NLr)Lr)'>o[>'> HOO p rH [>inininino^roi>in l>H O Di 1 W1 CQ 1 21 p1 51 ro ft ro rH o [> o i>!> O o o rH ro ro d o o Oo o o o o o o s 0 ro ro ro ro ro ro ro ro ro ro Pi o o o o o O O O cn cn H O UUuUUUUUUU 2 S N ft in o rH o in ro in in !> CO CO cn O O o o d o o o o O o o o rH rH 0 ro ro ro ro ro ro ro ro ro ro u O O cn O O O cn cn O O o u u U U U U U UUU 1562 ro LO co cn H (N O0I1 loI E-i ro lo 2 ro d) U > O U Individual Clinical Hematology Data oo\ ui C m o\ s Uol H H MONO% H U O O X O P i Pfc X H ^ ui i-HEh c o\ fP2Xf-il p i111 11i OHOOOHOOHO LD oo i--I rorOHCNCNrOHCN^H o ( NHOi--I cocnLO^Hco^cncNco rH 01 o ro o rH ^ O O ^ ^ L O C N C N C N C O [>Locococoi>coLr)Loco [> LO O O i> h h SEG% Q 1/1 3 Eh p i M^ !i [> U . O! is J 3. | X d) d) & oX(J^&_- ui o ii <|H PQX 111i Lncnrocnrororoi>(NCO lo co o i--li--li--I i--li--li--irocN i--I i--I o o o o o o o o o o o ooo ............... i--I CARCII SALT CO 1--d) rd Bd) En Q8 3^ 2P1w--1 K, Ul c Eh P1 5M ^3- 11 Wo q5 1i Wx ; i LD O O! LO Eh rlCNHi--I r ICNHi--I r I r I i--IOO ............... i--I U X o 8Q ftB P, *! ft rOLOroCNi-HLOrOLO-sF-sF ............... O i So i jj CO -sPi-HO i--I u^ O2 rH X 111 -H d P o 5 d) Ej ou OJ' c> cEh H p fPf-il\3. |11 2 X P ^1X-1 ii 11i Drdi C0O Q ' > L T )O [> (N (N L r)r0 (N C 0 ................................................... (N ^^^LnLOLO fOCN *^ i--I -sF-sFO I--I *=tfrH X H H & rd j-x ii o 10 W OJ i o i--l Ol 5X | o i--I i 1-- 1 d) > d) LO D a. (U-o !s O rH : 11 l p d) C0O ro 1 0 Xl i--I cn lo o rH LD H 01 i Wi C2Q i i Pi 5i LD ft o i--I ro CO ro l> o O! cn I--1ro ro ro LOLOLOLOl> d I--1i--l i--l i--l i--I i--1i--1i--1i--1i--I 0 ro ro ro ro ro ro ro ro ro ro H o o o o o o o o O O o UUUUUUUUUU 1563 Appendix 5 Individual Clinical Hematology Data Females Week 27 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ANISO POLY POIK HYPO TOXNEUT Group: 1 C92876 C92878 C92888 C92906 C92916 C92917 C92921 C92922 C92923 C92928 Group: 2 C92932 C92935 C92938 C92951 C92954 C92956 C92960 C92974 C92979 C92989 Dose Level: 0 -------------------- Dose Level: 0.5 --------------------- Dosage Unit: ppm T-6295 - - Dosage Unit: ppm T-6295 ----------- Covance 6329-183 3M T-6295 1564 Appendix 5 Individual Clinical Hematology Data Females Week 27 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ANISO POLY POIK HYPO TOXNEUT Group: 3 C93003 C93004 C93011 C93020 C93027 C93030 C93034 C93046 C93047 C93049 Group: 4 C93052 C93055 C93072 C93080 C93081 C93090 C93094 C93095 C93103 C93104 Dose Level: 2.0 --------------------- Dose Level: 5.0 Dosage Unit: ppm T-6295 ----------- Dosage Unit: ppm T-6295 Covance 6329-183 3M T-6295 1565 Appendix 5 Individual Clinical Hematology Data Females Week 27 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ANISO POLY POIK HYPO TOXNEUT Group: 5 C93119 C93131 C93133 C93138 C93149 C93153 C93157 C93160 C93162 C93179 Dose Level: 20.0 --------------------- Dosage Unit: ppm T-6295 ----------- Covance 6329-183 3M T-6295 1566 Appendix 5 Individual Clinical Hematology Data Males Week 53 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL RBC HGB HCT MCV MCH MCHC PLT NUMBER X106///L G/DL FL PG X103///L Group: 1 C92501 C92504 C92507 C92512 C92517 C92523 C92534 C92540 C92562 C92570 MEAN S.D. N Group: 2 C92573 C92587 C92592 C92596 C92604 C92612 C92614 C92624 C92625 C92629 MEAN S.D. N Dose Level: 0 8. 22 8.32 8.64 8.24 7.98 7.77 8.55 8.39 8.59 8.58 15.5 15.3 16.0 15.6 15.6 13.7 15.9 15.8 16.2 15.2 8.33 .286 10 15.5 .70 10 Dose Level: 0.5 8.44 8.55 8.73 8. 20 8.70 8.34 8.04 8.27 8.03 8.04 15.4 14.9 15.4 15.3 15.3 15.0 15.1 15.6 15.1 15.2 8.33 .266 10 15.2 .21 10 Dosage Unit: ppm T-6295 43.4 43.8 44.0 44.1 43.5 38.5 45.4 44.3 45.9 42.5 52.8 52.6 50.9 53.5 54.5 49.6 53.1 52.8 53.5 49.5 18.9 18.4 18.5 19.0 19.5 17.6 18.6 18.9 18.9 17.7 43.5 2. 02 10 52.3 1.70 10 18.6 .59 10 Dosage Unit: ppm T-6295 43.6 43.7 45.0 43.5 43.7 42.5 42.6 43.7 42.4 43.5 51.7 51.1 51.5 53.1 50.2 50.9 53.0 52.8 52.7 54.2 18.3 17.5 17.7 18.6 17.6 18.0 18.8 18.8 18.8 19.0 43.4 .77 10 52.1 1.23 10 18.3 .57 10 35.8 35.0 36.3 35.5 35.8 35.6 35.1 35.8 35.4 35.7 35.6 .38 10 35.3 34.2 34.3 35.1 35.0 35.4 35.4 35.6 35.6 35.0 35.1 .49 10 1161 971 1103 919 1206 1194 1234 890 1056 1388 1112 155. 10 992 1414 924 1049 887 1038 1124 955 1292 915 1059 172. 10 Covance 6329-183 3M T-6295 1567 Appendix 5 Individual Clinical Hematology Data Males Week 53 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL RBC HGB HCT MCV MCH MCHC PLT NUMBER X106/m L G/DL % FL PG % X 103/m L Group: 3 C92634 C92635 C92643 C92649 C92653 C92654 C92667 C92669 C92680 C92686 MEAN S.D. N Group: 4 C92694 C92704 C92710 C92711 C92727 C92729 C92739 C92741 C92742 C92748 MEAN S.D. N Dose Level: 2. 0 8.23 8.01 8.26 8.32 8.25 7.89 8.26 8.16 7.40 8.35 8.11 .288 10 14.2 15.8 15.2 14.8 14.9 14.1 15.5 15.6 14.0 15.0 14.9 .64 10 Dose Level: 5.0 8.47 7.88 8.59 8.36 9.14 7.29 8. 88 8.65 8.81 8. 20 15.2 14.8 14.7 14.7 15.7 14.7 15.2 15.6 15.9 15.1 8.43 .536 10 15.2 .45 10 Dosage Unit: ppm T-6295 41.4 44.7 43.0 42.3 42.2 40.1 43.8 44.2 40.0 43.5 50.3 55.8 52.0 50.8 51.1 50.9 53.0 54.2 54.0 52.1 17.3 19.8 18.4 17.8 18.0 17.9 18.8 19.1 18.9 18.0 42.5 1.63 10 52.4 1.79 10 18.4 .75 10 Dosage Unit: ppm T-6295 43.3 42.2 42.6 42.5 45.5 42.2 45.5 44.0 46.4 42.8 51.1 53.6 49.5 50.8 49.7 57.8 51.3 50.9 52.6 52.2 17.9 18.8 17.2 17.6 17.2 20.2 17.1 18.0 18.0 18.5 43.7 1.56 10 52.0 2.41 10 18.0 .94 10 34.4 35.5 35.4 35.1 35.2 35.1 35.4 35.3 34.9 34.6 35.1 .36 10 35.0 35.1 34.7 34.6 34.6 35.0 33.4 35.3 34.3 35.4 34.7 .58 10 913 830 944 938 1127 952 1053 838 963 752 931 108 10 832 1037 1199 1062 1001 900 1124 910 964 1078 1011 111 10 Covance 6329-183 3M T-6295 1568 Appendix 5 Individual Clinical Hematology Data Males Week 53 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL RBC HGB HCT MCV MCH MCHC PLT NUMBER X106/iL G/DL % FL PG % X103/iL Group: 5 C92751 C92754 C92755 C92778 C92790 C92795 C92796 C92817 C92820 MEAN S.D. N Dose Level: 20.0 7.98 8.70 8.74 8.14 8.46 8.28 7.85 7.81 8.60 14.3 15.6 15.9 14.3 15.2 14.3 13.9 14.8 15.5 8.28 .360 9 14.9 .71 9 Dosage Unit: ppm T-6295 40.3 43.1 44.0 40.6 43.3 40.5 38.8 42.4 42.6 50.5 49.6 50.3 49.9 51.2 48.9 49.5 54.2 49.5 17.9 18.0 18.1 17.6 17.9 17.3 17.7 19.0 18.0 41.7 1.74 9 50.4 1.58 9 17.9 .47 9 35.3 36.2 36.0 35.3 35.0 35.4 35.7 35.0 36.3 35.6 .49 9 1011 1367 1367 1378 1231 1186 1246 1114 1271 1241 124.3 9 Covance 6329-183 3M T-6295 1569 ANIMAL NUMBER Group: 1 C92501 C92504 C92507 C92512 C92517 C92523 C92534 C92540 C92562 C92570 MEAN S.D. N Group: 2 C92573 C92587 C92592 C92596 C92604 C92612 C92614 C92624 C92625 C92629 MEAN S.D. N Appendix 5 Individual Clinical Hematology Data Males Week 53 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS WBC X103///L N-SEG LYMPH X103///L X103///L MONO EOSIN X103///L X103///L BASO N -SEG% X103///L LYMPH% MONO% EOSIN% Covance 6329-183 3M T-6295 BASO% Dose Level : 0 Dosage Unit: ppm 'T-6295 6 . 1 1 . 8 3.1 1. 0 . 1 .0 29 52 17 1 1 6.7 3.2 2. 8 .6 . 1 .0 47 42 9 2 0 6. 6 1.3 4.4 .8 . 1 .0 19 66 12 1 1 6.3 2. 2 3.0 1 . 1 . 1 .0 34 47 17 1 0 4.6 .8 3.0 .7 . 1 .0 18 65 14 3 0 9.1 2.3 5.7 1. 0 .2 .0 25 62 11 2 0 8. 6 3.4 3.8 1.3 .2 .0 39 43 15 2 0 7.0 3.9 2.4 .6 . 1 .0 56 34 8 2 0 7.6 2. 6 3.9 1. 0 . 1 .0 34 52 13 2 0 8. 2 2.9 3.8 1.3 .2 .0 36 47 16 2 0 7.1 2.4 3.6 .9 . 1 .0 34 51 13 2 0 1.33 .96 .96 .26 .05 .00 1 1 ..9 1 0 ..6 3..2 .6 .4 10 10 10 10 10 10 10 10 10 10 10 Dose Level : 0.5 Dosage Unit: ppm 'T-6295 7.1 1 . 8 4.1 1. 0 . 1 .0 25 58 14 2 0 10. 2 2.3 6.4 1.3 .2 .0 22 63 13 2 0 9.4 1.9 6.0 1.3 . 1 .0 20 64 14 1 1 5.2 1.4 3.2 .5 .0 .0 27 62 10 1 0 6.7 1.5 4.1 1. 0 . 1 .0 22 61 15 2 0 8.0 1.3 5.6 .9 .2 .0 16 70 12 2 0 6. 2 1.4 3.6 1 . 1 . 1 .0 22 58 17 2 0 8.3 1.7 5.6 .9 . 1 .0 20 68 11 1 0 9.6 1.9 5.7 1.7 .2 . 1 20 59 18 3 1 7.8 1 . 8 4.9 1. 0 . 1 .0 22 63 13 1 0 7.8 1.7 4.9 1 . 1 . 1 .0 22 63 14 2 0 1.59 .31 1 . 1 0 .32 .06 .03 3..0 4..0 2 .5 .7 .4 10 10 10 10 10 10 10 10 10 10 10 1570 ANIMAL NUMBER Group: 3 C92634 C92635 C92643 C92649 C92653 C92654 C92667 C92669 C92680 C92686 MEAN S.D. N Group: 4 C92694 C92704 C92710 C92711 C92727 C92729 C92739 C92741 C92742 C92748 MEAN S.D. N Appendix 5 Individual Clinical Hematology Data Males Week 53 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS WBC X103///L N-SEG LYMPH X103///L X103///L MONO EOSIN X103///L X103///L BASO N-SEG% X103/iL LYMPH% MONO% EOSIN% Covance 6329-183 3M T-6295 BASO% Dose Level : 2. 0 Dosage Unit: ppm T-6295 7.6 2. 0 4.6 .9 . 1 .0 26 61 11 1 0 7.1 1.3 4.8 .9 . 1 .0 18 67 13 1 0 16.6 6.7 7.2 2. 6 . 1 . 1 40 43 15 1 0 7.4 1 . 6 5.7 .0 . 1 .0 21 77 01 0 9.0 1.5 6 . 1 1 . 2 . 1 .0 16 68 14 1 0 6.7 1 . 8 3.7 1. 0 .2 .0 26 55 16 2 0 8.8 1 . 1 6.8 .7 .1 .0 12 78 8 1 0 6.3 1 . 6 3.8 .8 . 1 .0 25 61 12 2 0 7.4 1.5 4.9 .9 . 1 .0 20 66 12 1 0 9.1 1 . 8 6 . 1 1 . 1 .2 .0 20 67 12 2 0 8.6 2 . 1 5.4 1. 0 .1 .0 22 64 11 1 0 2.97 1.64 1.20 .65 .04 .03 7.6 10. 2 4.5 .5 .0 10 10 10 10 10 10 10 10 10 10 10 Dose Level: 5.0 Dosage Unit: ppm T-6295 9.2 2.3 5.4 1.3 .2 .0 25 58 14 2 0 8.5 1 . 6 6.0 .8 . 1 .0 19 70 10 1 0 6.8 2 . 1 4.1 .5 . 1 .0 31 60 7 2 0 6.9 1 . 6 4.4 .7 . 1 .0 23 64 11 2 0 9.0 2. 2 5.3 1.3 .2 .0 25 58 14 2 0 7.4 1.7 4.8 .7 . 1 .0 23 66 9 2 0 11.7 3.9 6. 2 1.5 . 1 .0 33 52 13 1 0 10.5 1 . 2 8.4 .7 . 1 .0 11 80 7 1 0 8.4 1.7 5.6 .9 . 1 .0 21 67 11 1 0 7.0 1.9 4.1 .8 . 1 .0 27 59 12 2 0 8.5 2. 0 5.4 .9 . 1 .0 24 63 11 2 0 1.63 .74 1.28 .33 .04 .00 6. 2 7.9 2. 6 .5 .0 10 10 10 10 10 10 10 10 10 10 10 1571 ANIMAL NUMBER Group: 5 C92751 C92754 C92755 C92778 C92790 C92795 C92796 C92817 C92820 MEAN S.D. N Appendix 5 Individual Clinical Hematology Data Males Week 53 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS WBC X103//2L N-SEG LYMPH X103//2L X103//2L MONO EOSIN X103//2L X103//2L BASO N-SEG% X103//2L LYMPH% MONO% EOSIN% Covance 6329-183 3M T-6295 BASO% Dose Level : 20.0 Dosage Unit: ppm T-6295 7.9 1.5 6.3 .0 . 1 .0 19 80 01 0 10.5 6.3 2. 6 1.4 . 1 . 1 60 25 13 1 1 7.8 1 . 8 4.7 1 . 1 .2 .0 24 60 14 2 0 7.4 2.4 3.8 1. 0 . 1 .0 32 52 14 1 0 4.7 1. 0 3.0 .5 . 1 .0 22 64 12 2 0 6.8 1 . 8 4.0 .8 . 1 .0 27 59 12 2 0 6. 6 2.4 3.0 1 . 2 . 1 .0 36 45 18 1 0 8. 6 2 . 1 5.5 .8 . 1 .0 25 64 10 2 0 5.0 1.3 2.9 .7 . 1 .0 26 58 14 2 0 7.3 2.3 4.0 .8 . 1 .0 30 56 12 2 0 1.78 1.58 1.29 .42 .03 .03 12.3 15.1 5.0 .5 .3 99999999999 1572 Appendix 5 Individual Clinical Hematology Data Males Week 53 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ANISO POLY POIK HYPO TOXNEUT Group: 1 C92501 C92504 C92507 C92512 C92517 C92523 C92534 C92540 C92562 C92570 Group: 2 C92573 C92587 C92592 C92596 C92604 C92612 C92614 C92624 C92625 C92629 Dose Level: 0 -------------------- Dose Level: 0.5 --------------------- Dosage Unit: ppm T-6295 - - Dosage Unit: ppm T-6295 ----------- Covance 6329-183 3M T-6295 1573 Appendix 5 Individual Clinical Hematology Data Males Week 53 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ANISO POLY POIK HYPO TOXNEUT Group: 3 C92634 C92635 C92643 C92649 C92653 C92654 C92667 C92669 C92680 C92686 Group: 4 C92694 C92704 C92710 C92711 C92727 C92729 C92739 C92741 C92742 C92748 Dose Level: 2.0 -------------------- Dose Level: 5.0 --------------------- Dosage Unit: ppm T-6295 - - Dosage Unit: ppm T-6295 ----------- Covance 6329-183 3M T-6295 1574 Appendix 5 Individual Clinical Hematology Data Males Week 53 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ANISO POLY POIK HYPO TOXNEUT Group: 5 C92751 C92754 C92755 C92778 C92790 C92795 C92796 C92817 C92820 Dose Level: 20.0 ------------------- Dosage Unit: ppm T-6295 ---------- Covance 6329-183 3M T-6295 1575 Appendix 5 Individual Clinical Hematology Data Females Week 53 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL RBC HGB HCT MCV MCH MCHC PLT NUMBER X106/m L G/DL % FL PG % X 103/m L Group: 1 C92862 C92876 C92889 C92894 C92899 C92910 C92917 C92922 C92923 C92930 MEAN S.D. N Group: 2 C92931 C92938 C92946 C92952 C92954 C92958 C92964 C92972 C92985 C92990 MEAN S.D. N Dose Level: 0 8.39 7.33 7.40 7.72 7.01 7.77 7.24 7.40 6.99 6.66 16.3 14.5 14.3 14.6 13.9 14.9 14.6 13.9 13.9 13.7 7.39 .485 10 14.5 .76 10 Dose Level: 0.5 6.83 7.47 8.40 6.79 7.18 8.28 6.23 6.61 7.25 7.53 14.2 13.9 16.4 13.3 14.1 15.5 12.9 13.9 13.6 14.5 7.26 .695 10 14.2 1.04 10 Dosage Unit: ppm T-6295 44.6 40.6 40.3 41.0 39.0 41.9 40.9 38.9 39.2 38.3 53.2 55.4 54.5 53.2 55.6 54.0 56.6 52.6 56.0 57.6 19.4 19.7 19.3 18.9 19.9 19.1 20.2 18.7 19.9 20.6 40.5 1.84 10 54.9 1.64 10 19.6 .60 10 Dosage Unit: ppm T-6295 40.1 40.4 48.0 38.3 40.1 44.3 37.5 39.7 39.5 42.3 58.8 54.1 57.1 56.3 55.8 53.5 60.2 60.1 54.4 56.2 20.8 18.5 19.5 19.6 19.6 18.7 20.8 21.1 18.8 19.2 41.0 3.11 10 56.6 2.40 10 19.7 .94 10 36.5 35.6 35.5 35.5 35.7 35.4 35.7 35.6 35.5 35.7 35.7 .31 10 35.4 34.3 34.2 34.9 35.1 34.9 34.5 35.0 34.5 34.2 34.7 .42 10 1127 995 888 1055 1010 988 1146 947 992 941 1009 80 10 874 1010 907 1068 806 845 1012 758 938 858 908 99 10 Covance 6329-183 3M T-6295 1576 Appendix 5 Individual Clinical Hematology Data Females Week 53 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL RBC HGB HCT MCV MCH MCHC PLT NUMBER X106/m L G/DL % FL PG % X 103/m L Group: 3 C92992 C92997 C93012 C93022 C93027 C93029 C93032 C93042 C93046 C93048 MEAN S.D. N Group: 4 C93052 C93054 C93060 C93078 C93081 C93083 C93091 C93095 C93097 C93110 MEAN S.D. N Dose Level: 2. 0 6.95 7.11 6.18 7.83 7.97 7.28 7.35 6.87 7.62 7.97 14.5 14.2 13.1 14.8 14.8 14.2 14.8 13.5 14.6 14.9 7.31 .566 10 14.3 .61 10 Dose Level: 5.0 7.84 7.92 7.05 7.08 8.06 6.87 7.24 7.27 7.33 6.58 14.4 14.7 14.2 13.7 15.2 14.5 14.1 14.3 14.3 13.1 7.32 .479 10 14.2 .56 10 Dosage Unit: ppm T-6295 41.2 41.7 37.4 42.3 43.1 40.8 41.4 38.2 42.0 43.1 59.2 58.6 60.5 54.0 54.1 56.0 56.3 55.5 55.1 54.1 20.9 20.0 21.2 19.0 18.6 19.5 20.1 19.6 19.2 18.6 41.1 1.91 10 56.3 2.32 10 19.7 .89 10 Dosage Unit: ppm T-6295 41.6 42.6 40.7 39.9 44.1 41.0 40.5 41.4 41.8 37.5 53.0 53.8 57.7 56.4 54.7 59.7 56.0 56.9 57.0 57.0 18.3 18.5 20.1 19.4 18.8 21.1 19.5 19.7 19.5 20.0 41.1 1.73 10 56.2 1.96 10 19.5 .83 10 35.2 34.2 35.1 35.1 34.4 34.9 35.7 35.3 34.9 34.5 34.9 .45 10 34.6 34.4 34.9 34.3 34.4 35.4 34.9 34.6 34.1 35.1 34.7 .40 10 858 940 799 724 896 946 850 659 847 988 851 101 10 799 828 842 788 1042 787 883 741 774 888 837 85 10 Covance 6329-183 3M T-6295 1577 Appendix 5 Individual Clinical Hematology Data Females Week 53 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL RBC HGB HCT MCV MCH MCHC PLT NUMBER X106///L G/DL FL PG X103///L Group: 5 C93112 C93116 C93117 C93120 C93130 C93138 C93147 C93154 C93163 C93171 MEAN S.D. N Dose Level: 20.0 6.23 7.17 7.17 5.39 7.58 7.64 8.64 6.68 7.34 6.66 12.0 14.3 13.6 10.5 14.7 14.1 16.6 12.4 13.9 13.1 7.05 .881 10 13.5 1.66 10 Dosage Unit: ppm T-6295 33.9 40.2 38.3 29.9 41.0 39.8 46.8 35.3 38.9 36.5 54.5 56.1 53.4 55.4 54.1 52.0 54.2 52.8 53.0 54.7 19.2 20.0 18.9 19.4 19.4 18.5 19.2 18.6 18.9 19.6 38.1 4.56 10 54.0 1.25 10 19.2 .46 10 35.3 35.6 35.4 35.0 35.9 35.5 35.4 35.2 35.7 35.9 35.5 .29 10 1029 819 1053 1058 1077 849 1142 1083 1158 964 1023 113.7 10 Covance 6329-183 3M T-6295 1578 ANIMAL NUMBER Group: 1 C92862 C92876 C92889 C92894 C92899 C92910 C92917 C92922 C92923 C92930 MEAN S.D. N Group: 2 C92931 C92938 C92946 C92952 C92954 C92958 C92964 C92972 C92985 C92990 MEAN S.D. N Appendix 5 Individual Clinical Hematology Data Females Week 53 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS WBC X103///L N-SEG LYMPH X103///L X103///L MONO EOSIN X103///L X103///L BASO N-SEG% X103///L LYMPH% MONO% EOSIN% Covance 6329-183 3M T-6295 BASO% Dose Level : 0 Dosage Unit: ppm T-6295 3.1 1.4 1 . 2 .4 . 1 .0 46 39 13 2 0 3.6 1. 0 2. 2 .4 . 1 .0 27 60 11 2 0 4.7 2.4 1.9 .4 . 1 .0 50 41 7 2 0 3.5 1. 0 1 . 8 .5 . 1 .0 30 52 13 4 0 5.6 2.5 2.3 .8 . 1 .0 44 41 14 2 0 3.1 .9 1 . 6 .5 . 1 .0 29 52 16 2 0 2 . 1 .6 1 . 1 .3 . 1 .0 30 50 16 3 0 3.0 .8 1 . 8 .3 . 1 .0 28 60 9 3 0 4.7 1.5 2. 6 .5 . 1 .0 32 55 10 2 0 8. 1 4.4 2.9 .7 . 1 .0 55 36 8 1 0 4.2 1 . 6 1.9 .5 . 1 .0 37 49 12 2 0 1.72 1.16 .57 .16 .00 .00 10.5 8. 8 3.2 .8 .0 10 10 10 10 10 10 10 10 10 10 10 Dose Level: 0.5 Dosage Unit:: ppm T-6295 4.3 .8 2.7 .8 . 1 .0 18 62 18 2 0 3.9 .8 2.5 .6 . 1 .0 20 62 15 2 0 7.9 1.4 5.8 .6 . 1 .0 18 73 8 2 0 3.5 .9 2. 0 .5 .0 .0 26 57 14 1 0 5.4 1.5 3.2 .5 . 1 .0 28 60 10 2 0 4.6 1. 0 2. 6 .9 . 1 .0 22 56 19 2 1 5.2 1 . 1 3.6 .4 . 1 .0 21 69 7 3 0 2.5 .8 1.4 .3 . 1 .0 30 54 12 4 0 3.5 .6 2.4 .4 . 1 .0 16 69 12 3 0 6.0 1 . 1 4.4 .3 . 1 .0 19 74 5 2 0 4.7 1. 0 3.1 .5 . 1 .0 22 64 12 2 0 1.53 .28 1.27 .20 .03 .00 4.7 7.2 4.6 .8 .3 10 10 10 10 10 10 10 10 10 10 10 1579 ANIMAL NUMBER Group: 3 C92992 C92997 C93012 C93022 C93027 C93029 C93032 C93042 C93046 C93048 MEAN S.D. N Group: 4 C93052 C93054 C93060 C93078 C93081 C93083 C93091 C93095 C93097 C93110 MEAN S.D. N Appendix 5 Individual Clinical Hematology Data Females Week 53 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS WBC X103///L N-SEG LYMPH X103///L X103//L MONO EOSIN X103///L X103//L BASO N-SEG% X103/iL LYMPH% MONO% EOSIN% Covance 6329-183 3M T-6295 BASO% Dose Level : 2. 0 Dosage Unit: ppm T-6295 5.2 2 . 1 2.3 .7 . 1 .0 40 43 14 3 0 6 . 1 2.4 2.9 .6 . 1 .0 40 48 10 2 0 7.4 1 . 6 5.0 .7 .2 .0 21 68 9 2 0 5.0 .9 3.5 .4 . 1 .0 19 70 9 2 0 5.3 1.4 3.4 .4 . 1 .0 26 64 8 3 0 4.7 1 . 2 2.7 .7 .2 .0 25 57 15 4 0 4.8 1. 0 3.2 .5 . 1 .0 21 66 10 2 0 6.7 2. 0 3.9 .6 .2 .0 31 58 9 3 0 2. 8 .9 1 . 6 .2 . 1 .0 31 59 7 3 0 4.4 1 . 2 2.5 .5 . 1 .0 28 58 11 2 0 5.2 1.5 3.1 .5 . 1 .0 28 59 10 3 0 1.28 .54 .94 .16 .05 .00 7.4 8. 6 2.5 .7 .0 10 10 10 10 10 10 10 10 10 10 10 Dose Level: 5.0 Dosage Unit:: ppm T-6295 6. 6 2. 2 3.6 .6 . 1 .0 34 55 9 2 0 4.8 1. 0 3.0 .7 . 1 .0 21 62 14 3 1 3.6 .9 2.4 .3 .0 .0 26 66 7 1 0 3.8 1. 0 2. 0 .6 . 1 .0 27 53 16 3 0 3.8 .6 2. 6 .4 . 1 .0 16 70 11 3 0 2.5 .4 1 . 8 .2 .0 .0 16 74 8 2 0 5.4 1 . 2 3.3 .8 . 1 .0 22 60 16 2 0 4.0 1 . 2 2.3 .5 . 1 .0 29 57 13 2 0 2.7 .9 1.3 .4 . 1 .0 34 48 14 3 0 5.2 2 . 1 2.7 .3 . 1 .0 41 53 5 1 0 4.2 1 . 2 2.5 .5 . 1 .0 27 60 11 2 0 1.27 .58 .70 .19 .04 .00 8. 2 8. 2 3.9 .8 .3 10 10 10 10 10 10 10 10 10 10 10 1580 ro LD co cn H (N I u) O01 I E-i 2ro ro d) U > O U Individual Clinical Hematology Data OOOOOOOHOH oo o \ rH O UI C m o \ s UoI H H MONO% H U O O Ctf O PPfc Ctf W ^ C/0 i-HEh c o\ fP2Xf-ii p i i111 11i SEG% XM Q B 1/1 3 Eh p i M^ i! ro U . LD is P 3. | d) d) oX(J o^ & ui o ii <|H PQX 111i HroCNHroroCNHCN*^ o (NHO rH C ncn^CnCNCnLnC NCNCn rH rH rH rH LD ocno rH rH L n c n 'X '^ 'X 'ro (N l> 'X '(N s^ L T )L r)(N 'X 'l> c o ro ^ ^ ro l> o ld H H ^ C n O 'X 'C n 'X 'rH C n cn ^ s^ C N C N 'X 'rH rH rH ^ ro ^ rH ^ i> o ro H H oooooooooo ............................................................................. o ooo rH CARCII SALT fc P 1 CO d) i-- rti Bd) Q8 3^ 2P1--1 Ui K,W cc P Eh 5M ^3- 11 Wo q5 1i Wx ; i LD O O! U) Eh r lr lr lr lr lr lr lr ltN O ) ............................................................................. U X o 8Q ftB P , ft ^ r o ' X ' C n ^ r o r o o c o ^ *! O i So i 4P u^ O2 rH X 111 -H d P rH O O rH o 5 d) Ej ou OJ' c> cEh W p fPf-iI\3. 111 2 X P ^1X-1 ii 11i Di rti C0O o O C O L D r O r lr lC O r lr lO W Ol O .............................................................................................. rH ( N H C N C N C N C N ^ r o r O C N CN ji r i -x i Wi ui 5 X| i D 3. (U -o !s O rH 1 1 1 1 X1 o cn LD O O! rH Ol O! O O! 1--1 d) > d) 0 o LD o \ <N O CO ro cn p ro o \ ro O ! U ) CO K0 d) CO 0 O o rH O! rH ro ro O rH LD O! LD 01 1 H1 CQ i ft O l [> o o CO1> ro rH rH rH rH O ! ro ro LD ^ 0 1> d rH rH rH rH rH rH rH rH rH rH s 2 i 0 ro ro ro ro ro ro ro ro ro ro P i U O O O cn O O O cn cn cn H 5 i O u u UUUUUUUU 2 w 1581 Appendix 5 Individual Clinical Hematology Data Females Week 53 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ANISO POLY POIK HYPO TOXNEUT Group: 1 C92862 C92876 C92889 C92894 C92899 C92910 C92917 C92922 C92923 C92930 Group: 2 C92931 C92938 C92946 C92952 C92954 C92958 C92964 C92972 C92985 C92990 Dose Level: 0 -------------------- Dose Level: 0.5 --------------------- Dosage Unit: ppm T-6295 - - Dosage Unit: ppm T-6295 ----------- Covance 6329-183 3M T-6295 1582 Appendix 5 Individual Clinical Hematology Data Females Week 53 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ANISO POLY POIK HYPO TOXNEUT Group: 3 C92992 C92997 C93012 C93022 C93027 C93029 C93032 C93042 C93046 C93048 Group: 4 C93052 C93054 C93060 C93078 C93081 C93083 C93091 C93095 C93097 C93110 Dose Level: 2.0 -------------------- Dose Level: 5.0 --------------------- Dosage Unit: ppm T-6295 - - Dosage Unit: ppm T-6295 ----------- Covance 6329-183 3M T-6295 1583 Appendix 5 Individual Clinical Hematology Data Females Week 53 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER ANISO POLY POIK HYPO TOXNEUT Group: 5 C93112 C93116 C93117 C93120 C93130 C93138 C93147 C93154 C93163 C93171 Dose Level: 20.0 ----------- Dosage Unit: ppm T-6295 --------------------- Covance 6329-183 3M T-6295 1584 Appendix 5 Individual Clinical Chemistry Data Males Week 4 104 WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER GLU MG/DL UN MG/DL CREAT MG/DL T PRO G/DL ALB G/DL GLOB G/DL T BILI MG/DL CHOL MG/DL Group: 1 Dose Level: 0 Dosage Unit: ppm T-6295 C92505 C92506 C92524 C92526 C92529 C92542 C92543 C92546 C92548 C92553 88 13 .6 5.5 4.2 1.3 . 1 45 114 11 .7 5.9 3.8 2 . 1 .0 46 104 12 .7 6.8 4.5 2.3 . 1 52 113 14 .6 6. 2 4.5 1.7 .0 39 93 13 .5 6 . 1 4.5 1 . 6 .0 47 92 10 .7 5.9 4.3 1 . 6 . 1 53 96 11 .5 6.3 4.5 1 . 8 .3 51 95 13 .8 6.9 4.8 2 . 1 . 1 62 98 11 .4 6.0 4.5 1.5 . 1 54 76 12 .2 5.9 4.4 1.5 . 1 43 MEAN S.D. N 97 12 .6 6. 2 4.4 1 . 8 . 1 49 11.4 1 .2 .18 .43 .26 .32 .09 6 10 10 10 10 10 10 10 10 Group: 2 Dose Level: 0.5 Dosage Unit: ppm T-6295 C92573 C92574 C92575 C92585 C92586 C92587 C92610 C92613 C92615 C92618 MEAN S.D. N 98 10 .7 6 . 1 4.4 1.7 .2 47 97 12 .6 6.7 4.7 2. 0 . 1 36 91 16 .7 6. 6 4.1 2.5 .0 54 91 14 .7 5.9 4.1 1 . 8 .2 48 93 13 .6 6. 2 4.6 1 . 6 .0 42 104 12 .6 6 . 1 4.6 1.5 .2 48 91 12 .6 6. 6 4.8 1 . 8 .0 56 105 12 .6 6. 6 4.8 1 . 8 . 1 53 98 12 .6 6. 2 4.8 1.4 .2 57 99 10 .5 6.9 4.9 2. 0 .0 47 97 12 .6 6.4 4.6 1 . 8 . 1 49 5.2 1 .8 .06 .33 .29 .31 .09 6 10 10 10 10 10 10 10 10 Covance 6329-183 3M T-6295 1585 Appendix 5 Individual Clinical Chemistry Data Males Week 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER GLU MG/DL UN MG/DL CREAT MG/DL T PRO G/DL ALB G/DL GLOB G/DL T BILI MG/DL CHOL MG/DL Group: 3 Dose Level: 2. 0 Dosage Unit: ppm T-6295 C92632 C92646 C92650 C92652 C92657 C92668 C92676 C92678 C92684 C92686 93 11 .5 5.9 4.4 1.5 . 1 56 103 11 .7 6.5 4.9 1 . 6 . 1 51 82 10 .6 6. 6 5.0 1 . 6 .2 39 98 12 .7 6.3 4.7 1 . 6 . 1 47 89 12 .9 5.8 4.5 1.3 .2 44 108 13 .7 6. 2 4.3 1.9 . 1 45 93 13 .5 5.7 4.2 1.5 .0 25 70 14 .5 6.0 4.3 1.7 .0 38 91 14 .4 5.8 4.3 1.5 .0 50 85 12 .6 5.7 4.2 1.5 .2 44 MEAN S.D. N 91 12 .6 6.0 4.5 1 . 6 . 1 44 10. 8 1.3 .14 .33 .29 .16 .08 8. 6 10 10 10 10 10 10 10 10 Group: 4 Dose Level: 5. 0 Dosage Unit: ppm T-6295 C92709 C92715 C92716 C92718 C92725 C92731 C92732 C92734 C92744 C92746 87 15 .6 6.5 4.7 1 . 8 . 1 67 105 13 .7 6.8 4.9 1.9 . 1 53 92 12 .5 6. 2 4.6 1 . 6 . 1 36 95 16 .7 6.5 4.8 1.7 .0 35 78 13 .6 5.9 4.5 1.4 .0 55 92 13 .5 6.3 4.7 1 . 6 .0 22 86 10 .6 5.9 4.4 1.5 .0 35 108 10 .7 6 . 1 4.3 1 . 8 .0 53 95 10 .8 6.5 4.8 1.7 .0 70 99 13 .7 6.3 4.5 1 . 8 . 1 52 MEAN S.D. N 94 12 .6 6.3 4.6 1.7 .0 48 8.9 2 . 1 .10 .29 .19 .15 .05 15.3 10 10 10 10 10 10 10 10 Covance 6329-183 3M T-6295 1586 Appendix 5 Individual Clinical Chemistry Data Males Week 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER GLU MG/DL UN MG/DL CREAT MG/DL T PRO G/DL ALB G/DL GLOB G/DL T BILI MG/DL CHOL MG/DL Group: 5 Dose Level: 20.0 Dosage Unit: ppm T-6295 C92752 C92759 C92779 C92784 C92793 C92798 C92799 C92805 C92807 C92811 91 15 .5 6.5 4.9 1 . 6 . 1 41 89 14 .6 6.4 4.8 1 . 6 .1 21 74 13 .6 6. 6 4.7 1.9 .0 58 79 13 .6 6.3 4.6 1.7 .0 60 83 11 .3 6.0 4.5 1.5 .0 44 82 11 .5 6.4 4.6 1 . 8 . 1 60 88 15 .5 6.3 4.6 1.7 . 1 27 83 14 .5 5.9 4.3 1 . 6 .3 36 85 15 .7 6. 2 4.4 1 . 8 . 1 50 81 13 .7 6.0 4.5 1.5 .0 51 MEAN S.D. N 84 13 .6 6.3 4.6 1.7 . 1 45 5.0 1.5 .12 .23 .18 .13 .09 13.7 10 10 10 10 10 10 10 10 Covance 6329-183 3M T-6295 1587 Appendix 5 Individual Clinical Chemistry Data Males Week 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER AST/SGOT ALT/SGPT IU/L IU/L GGT IU/L CA MG/DL I PHOS MG/DL NA MMOL/L K MMOL/L CL MMOL/L Group: 1 C92505 C92506 C92524 C92526 C92529 C92542 C92543 C92546 C92548 C92553 MEAN S.D. N Group: 2 C92573 C92574 C92575 C92585 C92586 C92587 C92610 C92613 C92615 C92618 MEAN S.D. N Dose Level: 0 107 104 104 72 153 157 122 125 146 134 122 26.4 10 33 26 31 31 37 27 36 30 32 31 31 3. 4 10 Dose Level: 0.5 135 29 133 27 161 35 135 29 121 34 148 28 177 32 110 35 130 41 207 33 146 28.9 10 32 4. 2 10 Dosage Unit: ppm T-6295 0 1 1 2 0 1 4 0 0 0 1 1.3 10 10.3 11.0 10.9 11.1 10.3 10.7 10.4 11.5 10.3 10.7 10.7 .41 10 9.3 10.4 10.5 10.2 9.7 10.5 9.5 10.5 9.1 9.9 10.0 .54 10 Dosage Unit: ppm T-6295 0 10.4 8.9 0 10.8 10.2 1 10.7 10. 0 0 10.5 9.3 0 10.5 9.1 0 10.5 8.7 2 11.0 10.5 0 11.4 10.0 0 10.6 9.0 0 10.9 9.6 0 .7 10 10.7 .31 10 9.5 .62 10 148 150 147 145 147 146 148 150 146 148 148 1 .6 10 5.5 7.1 6.5 6.4 6.0 5.7 6.5 7.7 5.7 6.0 6.3 .69 10 146 147 149 145 146 146 147 148 147 149 147 1 .3 10 5.5 6.4 6.8 6.7 6.4 6.2 6.4 6.3 5.6 6.4 6.3 .42 10 105 106 103 101 102 104 104 105 104 104 104 1 10 103 105 105 102 104 102 104 104 103 104 104 1 10 Covance 6329-183 3M T-6295 1588 Appendix 5 Individual Clinical Chemistry Data Males Week 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER AST/SGOT ALT/SGPT IU/L IU/L GGT IU/L CA MG/DL I PHOS MG/DL NA MMOL/L K MMOL/L CL MMOL/L Group: 3 C92632 C92646 C92650 C92652 C92657 C92668 C92676 C92678 C92684 C92686 MEAN S.D. N Group: 4 C92709 C92715 C92716 C92718 C92725 C92731 C92732 C92734 C92744 C92746 MEAN S.D. N Dose Level: 2.0 119 28 94 24 115 30 79 22 119 40 64 28 89 23 103 30 124 32 137 28 104 2 2 .6 10 28 5.2 10 Dose Level: 5.0 110 31 83 28 114 29 126 36 142 36 106 28 137 31 104 24 116 27 107 30 114 17.2 10 30 3.8 10 Dosage Unit: ppm T-6295 0 0 0 0 0 4 0 1 4 1 1 1.6 10 10.8 10.8 10.7 11.4 10.1 10.9 10.2 10.5 10.2 10.3 10.6 .41 10 9.6 11.0 10.2 10.3 9.8 10.1 10.1 10.2 9.7 10.1 10.1 .39 10 Dosage Unit: ppm T-6295 2 11.1 10.1 0 11.1 9.7 0 10.9 10. 2 1 10.6 10.0 0 10.6 9.6 0 10.6 9.1 0 10.6 9.5 1 1 1 . 2 10.7 0 11.3 10.5 0 10.7 8.7 0 .7 10 10.9 .28 10 9.8 .62 10 148 149 148 148 145 149 148 146 148 146 148 1.4 10 5.5 7.5 6.6 6.0 5.4 6.6 6.6 5.6 6.5 5.3 6.2 .71 10 146 147 146 147 146 150 145 147 148 146 147 1.4 10 6.0 6.0 5.1 6.5 5.8 6.4 6.0 7.2 7.0 5.7 6.2 .62 10 104 103 105 106 103 105 106 105 105 104 105 1 10 99 104 101 104 102 107 103 105 103 104 103 2 10 Covance 6329-183 3M T-6295 1589 Appendix 5 Individual Clinical Chemistry Data Males Week 4 104 WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER AST/SGOT ALT/SGPT IU/L IU/L GGT IU/L CA MG/DL I PHOS MG/DL NA MMOL/L K MMOL/L CL MMOL/L Group: 5 C92752 C92759 C92779 C92784 C92793 C92798 C92799 C92805 C92807 C92811 MEAN S.D. N Dose Level: 20.0 122 30 170 33 121 35 143 33 95 33 118 28 145 38 137 33 129 29 133 34 131 19. 9 10 33 3.0 10 Dosage Unit: ppm T-6295 1 10.5 1 0 . 1 0 10.2 8.5 0 10.6 9.9 4 10.9 9.7 0 10.2 9.6 0 11.2 10.7 0 10.9 10.4 1 10.6 9.5 0 10.7 9.6 0 10.5 9.9 1 1.3 10 10.6 .31 10 9.8 .59 10 150 146 147 146 146 148 149 148 149 147 148 1.4 10 6.6 6.0 6.2 5.4 5.1 7.0 6.5 5.6 6.3 5.9 6.1 .58 10 104 103 104 102 102 104 103 105 103 103 103 .9 10 Covance 6329-183 3M T-6295 1590 Appendix 5 Individual Clinical Chemistry Data Females Week 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER GLU MG/DL UN MG/DL CREAT MG/DL T PRO G/DL ALB G/DL GLOB G/DL T BILI MG/DL CHOL MG/DL Group: 1 C92861 C92864 C92876 C92877 C92882 C92883 C92914 C92919 C92925 C92926 MEAN S.D. N Group: 2 C92941 C92945 C92950 C92953 C92959 C92964 C92968 C92969 C92976 C92982 MEAN S.D. N Dose Level: 0 110 12 115 12 105 14 111 12 106 14 127 14 118 16 123 14 1 1 1 17 112 13 114 7.1 10 14 1.7 10 Dose Level: 0.5 112 15 102 13 102 12 116 15 123 14 116 15 106 16 107 11 118 11 111 12 111 7.0 10 13 1.8 10 Dosage Unit: ppm T-6295 .5 6. 8 5.2 .7 6.9 5.3 .7 6.9 5.2 .7 7.0 5.1 .6 7.1 5.0 .8 7.1 5.2 .5 6.7 5.0 .7 7.1 5.2 .7 6.9 5.2 .7 6. 8 5.0 .7 6.9 5.1 .10 .14 .11 10 10 10 Dosage Unit: ppm T-6295 .9 7.0 5.1 .7 6.9 4.8 .7 7.0 4.8 .8 6. 6 4.9 .6 6.7 5.2 .8 7.0 5.4 .8 6. 8 5.1 .7 6. 6 5.0 .8 6.7 5.1 .9 6. 8 5.0 .8 6. 8 5.0 .09 .16 .18 10 10 10 1.6 1.6 1.7 1.9 2.1 1.9 1.7 1.9 1.7 1.8 1.8 .16 10 1.9 2.1 2.2 1.7 1.5 1.6 1.7 1.6 1.6 1.8 1.8 .23 10 .3 54 .0 89 .2 79 .0 74 . 1 87 .0 76 . 1 75 .2 66 . 1 58 . 1 65 . 1 72 .10 11.5 10 10 . 1 82 .0 65 .0 85 . 1 80 .0 27 . 1 49 .2 124 .0 58 .0 68 .0 58 .0 70 .07 25.8 10 10 Covance 6329-183 3M T-6295 1591 Appendix 5 Individual Clinical Chemistry Data Females Week 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER GLU MG/DL UN MG/DL CREAT MG/DL T PRO G/DL ALB G/DL GLOB G/DL T BILI MG/DL CHOL MG/DL Group: 3 Dose Level: 2. 0 Dosage Unit: ppm T-6295 C92993 C92996 C92998 C93004 C93006 C93008 C93023 C93031 C93033 C93045 106 13 .7 6.8 5.0 1 . 8 .2 75 107 16 .7 6. 6 4.9 1.7 .0 41 143 15 .9 7.0 5.1 1.9 .0 60 149 16 .7 7.0 5.1 1.9 .0 61 99 12 .7 6.5 5.0 1.5 . 1 32 101 13 .7 7.6 5.8 1 . 8 . 1 86 109 15 .9 6.8 5.0 1 . 8 .0 45 98 12 .5 6.3 5.0 1.3 . 1 79 109 17 .8 6.4 4.6 1 . 8 .0 73 109 13 .9 7.1 5.4 1.7 .0 55 MEAN S.D. N 113 14 .8 6.8 5.1 1.7 .0 61 17.9 1 . 8 .13 .39 .32 .19 .07 17.7 10 10 10 10 10 10 10 10 Group: 4 Dose Level: 5. 0 Dosage Unit: ppm T-6295 C93058 C93067 C93070 C93074 C93075 C93083 C93084 C93089 C93094 C93105 126 10 .8 7.3 5.2 2 . 1 .0 54 114 12 .9 7.6 5.7 1.9 . 1 59 105 12 .7 6.8 5.1 1.7 .0 49 125 14 .7 6.9 5.2 1.7 .0 82 105 13 .7 7.1 5.1 2. 0 . 1 42 110 12 .7 7.6 5.9 1.7 .0 78 92 14 .6 7.1 5.1 2. 0 .0 58 102 13 .7 7.2 5.3 1.9 .0 53 115 13 .8 6.8 5.1 1.7 .0 68 98 13 .7 6.5 4.9 1 . 6 .0 35 MEAN S.D. N 109 13 .7 7.1 5.3 1 . 8 .0 58 1 1 . 0 1 . 2 .08 .35 .31 .17 .04 14.8 10 10 10 10 10 10 10 10 Covance 6329-183 3M T-6295 1592 Appendix 5 Individual Clinical Chemistry Data Females Week 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER GLU MG/DL UN MG/DL CREAT MG/DL T PRO G/DL ALB G/DL GLOB G/DL T BILI MG/DL CHOL MG/DL Group: 5 Dose Level : 20.0 Dosage Unit: ppm T-6295 C93114 C93123 C93142 C93144 C93151 C93155 C93158 C93159 C93164 C93175 1 1 1 15 .8 7.4 5.4 2. 0 . 1 38 116 13 .6 7.0 5.2 1 . 8 .0 52 113 16 .6 7.2 5.4 1 . 8 .0 50 113 14 .8 6.5 5.0 1.5 . 1 52 100 12 .6 6.3 4.9 1.4 .3 65 105 14 .6 6. 6 5.1 1.5 .0 54 100 11 .6 7.0 5.3 1.7 .0 38 97 14 .6 6.5 5.0 1.5 .0 64 118 13 .8 6.8 5.1 1.7 .0 59 100 17 .6 7.0 5.4 1 . 6 .0 70 MEAN S.D. N 107 14 .7 6.8 5.2 1 . 6 .0 54 7.7 1 .8 .10 .35 .19 .18 .10 10.7 10 10 10 10 10 10 10 10 Covance 6329-183 3M T-6295 1593 Appendix 5 Individual Clinical Chemistry Data Females Week 4 104 WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER AST/SGOT ALT/SGPT IU/L IU/L GGT IU/L CA MG/DL I PHOS MG/DL NA MMOL/L K MMOL/L CL MMOL/L Group: 1 C92861 C92864 C92876 C92877 C92882 C92883 C92914 C92919 C92925 C92926 MEAN S.D. N Group: 2 C92941 C92945 C92950 C92953 C92959 C92964 C92968 C92969 C92976 C92982 MEAN S.D. N Dose Level: 0 148 28 143 27 121 29 99 40 108 33 104 28 143 29 96 38 93 36 177 36 123 28.2 10 32 4.8 10 Dose Level: 0. 5 199 34 172 44 124 30 120 32 99 23 104 26 103 27 106 31 86 30 86 30 120 37.2 10 31 5.6 10 Dosage Unit: ppm T-6295 0 10.6 8.3 0 10.4 7.7 0 10.9 8.2 1 10.5 7.6 1 10.6 7.3 2 11.1 8. 6 0 10.9 10.5 0 11.1 8. 8 0 11.2 8. 8 0 10.2 8. 8 0 .7 10 10.8 .34 10 8.5 .90 10 Dosage Unit: ppm T-6295 0 10.4 8.3 4 10.3 8.4 2 10.6 9.1 0 10.5 8. 0 1 10.5 6.7 0 11.2 7.1 0 11.0 9.2 2 10.3 1 0 . 1 2 10.9 7.8 0 10.5 9.1 1 1.4 10 10.6 .31 10 8.4 1.03 10 146 145 146 145 144 146 147 145 144 146 145 1.0 10 6.0 5.2 6.1 5.3 5.5 6.6 6.2 5.9 6.0 6.1 5.9 .43 10 144 146 146 146 145 145 145 147 145 145 145 .8 10 5.3 5.9 6.3 6.0 5.8 6.1 6.7 6.8 6.5 6.0 6.1 .45 10 106 104 102 105 104 103 105 104 104 106 104 1.3 10 104 105 104 104 105 103 103 105 104 106 104 .9 10 Covance 6329-183 3M T-6295 1594 Appendix 5 Individual Clinical Chemistry Data Females Week 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER AST/SGOT ALT/SGPT IU/L IU/L GGT IU/L CA MG/DL I PHOS MG/DL NA MMOL/L K MMOL/L CL MMOL/L Group: 3 C92993 C92996 C92998 C93004 C93006 C93008 C93023 C93031 C93033 C93045 MEAN S.D. N Group: 4 C93058 C93067 C93070 C93074 C93075 C93083 C93084 C93089 C93094 C93105 MEAN S.D. N Dose Level: 2. 0 96 96 115 86 104 124 94 85 104 104 101 12.2 10 31 19 34 24 25 28 31 26 36 36 29 5.6 10 Dose Level: 5.0 158 30 92 33 98 41 114 37 143 24 78 26 110 33 114 35 97 29 120 33 112 23.9 10 32 5.1 10 Dosage Unit: ppm T-6295 5 1 0 0 4 4 1 4 0 1 2 2.0 10 11.1 10.5 10.6 10.2 10.4 11.4 10.9 10.5 10.4 10.9 10.7 .37 10 8. 8 10.4 7.1 7.2 8.9 9.4 8.4 8.5 7.1 9.0 8.5 1.08 10 Dosage Unit: ppm T-6295 4 10.2 1 10.8 2 10.6 1 10.8 1 10.4 0 11.5 3 10.9 1 10.8 0 10.9 2 10.5 7.0 7.5 8.5 7.3 9.6 7.7 8.9 7.1 7.8 8. 0 2 1.3 10 10.7 .35 10 7.9 .83 10 145 147 147 146 148 149 147 147 145 148 147 1 .3 10 6.2 6.1 6.6 5.8 6.7 7.2 6.1 5.5 5.7 6.1 6.2 .51 10 146 150 147 146 150 145 148 146 145 143 147 2 .2 10 5.6 6.2 6.4 5.5 6.1 6.2 6.7 5.8 6.5 5.7 6.1 .41 10 104 105 105 105 107 105 104 107 106 107 106 1 10 103 105 107 104 107 104 105 105 103 103 105 1 10 Covance 6329-183 3M T-6295 1595 Appendix 5 Individual Clinical Chemistry Data Females Week 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER AST/SGOT ALT/SGPT IU/L IU/L GGT IU/L CA MG/DL I PHOS MG/DL NA MMOL/L K MMOL/L CL MMOL/L Group: 5 C93114 C93123 C93142 C93144 C93151 C93155 C93158 C93159 C93164 C93175 MEAN S.D. N Dose Level: 20.0 100 30 129 48 76 36 90 26 83 39 73 25 86 31 92 31 91 31 103 26 92 16. 0 10 32 7.1 10 Dosage Unit: ppm T-6295 0 10.7 1 1 . 1 0 10.3 6.4 0 10.6 6.9 0 10.9 10. 0 0 10.6 8. 0 2 10.6 7.8 3 10.6 7.9 0 10.3 8. 0 1 10.6 6.7 0 11.1 8.7 1 1.1 10 10.6 .24 10 8.2 1.47 10 150 147 146 148 144 145 145 145 146 145 146 1.8 10 6.2 5.6 5.2 7.2 5.9 5.7 6.0 5.9 5.6 6.5 6.0 .56 10 107 105 105 105 106 105 104 105 104 103 105 1.1 10 Covance 6329-183 3M T-6295 1596 Appendix 5 Individual Clinical Chemistry Data Males Week 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER PCOAO IU/G Group: 1 C92506 C92524 C92526 C92529 C92546 MEAN S.D. N Group: 2 C92574 C92575 C92610 C92613 C92618 MEAN S.D. N Group: 3 C92646 C92650 C92652 C92668 C92678 MEAN S.D. N Dose Level: 0 11 8 12 8 7 9 2.2 5 Dose Level: 0 8 12 10 10 6 9 2.3 5 Dose Level: 2 7 3 4 12 11 7 4.0 5 Dosage Unit: ppm T-6295 Dosage Unit: ppm T-6295 Dosage Unit: ppm T-6295 Covance 6329-183 3M T-6295 1597 Appendix 5 Individual Clinical Chemistry Data Males Week 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER PCOAO IU/G Group: 4 C92715 C92718 C92731 C92734 C92744 MEAN S.D. N Group: 5 C92752 C92759 C92779 C92793 C92798 MEAN S.D. N Dose Level: 5.0 9 7 7 8 8 8 .8 5 Dose Level: 20.0 4 6 6 8 6 6 1.4 5 Dosage Unit: ppm T-6295 Dosage Unit: ppm T-6295 Covance 6329-183 3M T-6295 1598 Appendix 5 Individual Clinical Chemistry Data Females Week 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER PCOAO IU/G Group: 1 C92861 C92864 C92914 C92919 C92926 MEAN S.D. N Group: 2 C92941 C92945 C92950 C92969 C92982 MEAN S.D. N Group: 3 C92996 C93006 C93008 C93031 C93045 MEAN S.D. N Dose Level: 0 4 4 5 7 3 5 1.5 5 Dose Level: 0.5 5 4 6 6 7 61.1 5 Dose Level: 2.0 3 1 3 5 1 3 1.7 5 Dosage Unit: ppm T-6295 Dosage Unit: ppm T-6295 Dosage Unit: ppm T-6295 Covance 6329-183 3M T-6295 1599 Appendix 5 Individual Clinical Chemistry Data Females Week 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER PCOAO IU/G Group: 4 C93058 C93067 C93070 C93075 C93084 MEAN S.D. N Group: 5 C93114 C93123 C93142 C93144 C93159 MEAN S.D. N Dose Level: 5.0 4 2 1 2 2 2 1.1 5 Dose Level: 20.0 4 3 5 4 6 4 1.1 5 Dosage Unit: ppm T-6295 Dosage Unit: ppm T-6295 Covance 6329-183 3M T-6295 1600 Appendix 5 Individual Clinical Chemistry Data Males Week 14 104 WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER GLU MG/DL UN MG/DL CREAT MG/DL T PRO G/DL ALB G/DL GLOB G/DL T BILI MG/DL CHOL MG/DL Group: 1 Dose Level: 0 Dosage Unit: ppm T-6295 C92508 C92509 C92511 C92521 C92527 C92528 C92532 C92535 C92537 C92562 MEAN S.D. N 106 10 .4 6.7 4.5 2. 2 .0 66 108 12 .6 6.9 4.8 2 . 1 .0 56 91 11 .4 7.2 5.2 2. 0 .0 64 108 14 .7 7.1 5.1 2. 0 . 1 47 103 11 .3 6. 6 4.8 1 . 8 .3 53 101 12 .4 7.4 5.2 2. 2 . 1 80 109 14 .6 7.6 5.0 2. 6 .2 74 97 16 .4 6. 6 4.5 2 . 1 . 1 42 101 14 .3 6.5 5.0 1.5 .6 81 92 13 .3 6.4 4.7 1.7 .2 68 102 13 .4 6.9 4.9 2. 0 .2 63 6.5 1 . 8 .14 .41 .26 .30 .18 13.4 10 10 10 10 10 10 10 10 Group: 2 Dose Level: 0.5 Dosage Unit: ppm T-6295 C92583 C92593 C92598 C92600 C92612 C92616 C92621 C92624 C92627 C92629 92 14 .6 7.2 5.0 2. 2 .3 52 117 17 .8 7.4 5.4 2. 0 .0 52 116 12 .6 7.2 4.9 2.3 .2 65 87 14 .6 7.2 5.1 2 . 1 .3 51 108 15 .3 6.9 4.9 2. 0 .2 40 99 14 .7 6. 6 5.1 1.5 . 1 33 117 13 .6 7.1 5.0 2 . 1 .0 91 104 11 .6 6.7 5.0 1.7 .0 41 101 14 .5 7.1 4.9 2. 2 .0 42 118 13 .4 6.3 4.6 1.7 .4 60 MEAN S.D. N 106 14 .6 7.0 5.0 2. 0 .2 53 1 1 . 2 1 . 6 .14 .34 .20 .26 .15 16.6 10 10 10 10 10 10 10 10 Covance 6329-183 3M T-6295 1601 Appendix 5 Individual Clinical Chemistry Data Males Week 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER GLU MG/DL UN MG/DL CREAT MG/DL T PRO G/DL ALB G/DL GLOB G/DL T BILI MG/DL CHOL MG/DL Group: 3 Dose Level: 2. 0 Dosage Unit: ppm T-6295 C92636 C92640 C92644 C92645 C92651 C92661 C92662 C92676 C92682 C92684 MEAN S.D. N 99 12 .4 6.7 4.6 2 . 1 .3 65 73 16 .6 6. 6 4.7 1.9 .0 40 99 17 .4 6.3 4.5 1 . 8 .3 32 99 13 .5 7.4 5.0 2.4 .0 76 106 13 .6 7.2 4.8 2.4 . 1 50 99 11 .4 6.5 4.6 1.9 .2 66 79 14 .6 6. 6 4.9 1.7 .2 50 65 14 .7 6.4 4.7 1.7 .0 34 94 13 .5 6.7 4.7 2. 0 . 1 42 99 11 .5 6.8 4.9 1.9 .0 51 91 13 .5 6.7 4.7 2. 0 . 1 51 13.7 2 .0 .10 .34 .16 .25 .12 14 10 10 10 10 10 10 10 10 Group: 4 Dose Level: 5.0 Dosage Unit: ppm T-6295 C92711 C92713 C92714 C92716 C92719 C92722 C92726 C92728 C92741 C92748 105 18 .4 6.7 4.7 2. 0 .2 48 105 14 .8 8.0 5.5 2.5 . 1 52 89 14 .6 6.9 4.9 2. 0 . 1 53 110 13 .6 6.7 4.8 1.9 .5 58 84 14 .5 6.8 4.7 2 . 1 .2 59 90 14 .6 6.9 4.9 2. 0 .4 70 107 15 .5 6.8 4.7 2 . 1 . 1 53 98 14 .4 7.6 5.3 2.3 .0 50 98 13 .5 6.5 4.6 1.9 .3 63 106 14 .6 7.2 4.9 2.3 .3 63 MEAN S.D. N 99 14 .6 7.0 4.9 2 . 1 .2 57 8.9 1 .4 .12 .46 .29 .20 .15 6 10 10 10 10 10 10 10 10 Covance 6329-183 3M T-6295 1602 Appendix 5 Individual Clinical Chemistry Data Males Week 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER GLU MG/DL UN MG/DL CREAT MG/DL T PRO G/DL ALB G/DL GLOB G/DL T BILI MG/DL CHOL MG/DL Group: 5 Dose Level: 20.0 Dosage Unit: ppm T-6295 C92754 C92765 C92767 C92774 C92777 C92784 C92789 C92799 C92812 C92820 100 19 .6 6.4 4.5 1.9 .2 41 85 17 .8 6.8 4.9 1.9 .0 39 105 14 .5 7.0 4.8 2. 2 .3 27 95 16 .5 6.5 4.7 1 . 8 .0 37 74 16 .7 6.9 5.1 1 . 8 .0 30 91 15 .6 7.2 5.0 2. 2 .3 45 96 18 .6 6.9 4.9 2. 0 . 1 18 1 1 1 17 .7 7.4 5.1 2.3 .0 29 96 16 .6 7.4 5.3 2 . 1 . 1 66 98 14 .2 6.8 4.7 2 . 1 .3 37 MEAN S.D. N 95 16 .6 6.9 4.9 2. 0 . 1 37 10.3 1 . 6 .16 .34 .24 .18 .13 12.9 10 10 10 10 10 10 10 10 Covance 6329-183 3M T-6295 1603 Appendix 5 Individual Clinical Chemistry Data Males Week 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER AST/SGOT ALT/SGPT IU/L IU/L GGT IU/L CA MG/DL I PHOS MG/DL NA MMOL/L K MMOL/L CL MMOL/L Group: 1 C92508 C92509 C92511 C92521 C92527 C92528 C92532 C92535 C92537 C92562 MEAN S.D. N Group: 2 C92583 C92593 C92598 C92600 C92612 C92616 C92621 C92624 C92627 C92629 MEAN S.D. N Dose Level: 0 116 73 149 128 122 83 110 89 125 116 111 23. 1 10 31 36 33 33 28 37 49 37 28 46 36 7.0 10 Dose Level: 0.5 123 40 115 48 92 34 104 40 122 51 114 43 140 32 102 36 131 48 77 39 112 18.8 10 41 6.3 10 Dosage Unit: ppm T-6295 1 1 0 0 4 0 0 4 1 1 1 1.5 10 10.1 11.1 10.6 10.5 10.2 10.7 10.4 10.4 10.5 10.4 10.5 .28 10 7.3 8.4 7.5 7.4 8.1 7.1 6.3 7.5 7.8 7.7 7.5 .57 10 Dosage Unit: ppm T-6295 1 10.4 1 11.0 1 11.0 0 10.6 1 10.2 2 10.6 0 10.5 1 10.1 0 10.5 0 10.4 7.9 8.5 8.3 8.9 7.6 7.2 7.0 7.4 7.1 7.6 1 .7 10 10.5 .29 10 7.8 .64 10 147 147 145 146 146 146 145 146 146 146 146 .7 10 6.7 6.2 6.1 6.3 6.1 6.6 5.5 5.7 6.3 6.0 6.2 .37 10 145 154 147 146 146 146 146 146 146 145 147 2.6 10 6.5 7.3 6.6 6.6 6.4 6.3 6.4 6.3 6.2 6.2 6.5 .32 10 106 106 103 107 104 105 104 105 106 104 105 1 10 102 108 102 103 104 107 105 105 106 104 105 2 10 Covance 6329-183 3M T-6295 1604 Appendix 5 Individual Clinical Chemistry Data Males Week 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER AST/SGOT ALT/SGPT IU/L IU/L GGT IU/L CA MG/DL I PHOS MG/DL NA MMOL/L K MMOL/L CL MMOL/L Group: 3 C92636 C92640 C92644 C92645 C92651 C92661 C92662 C92676 C92682 C92684 MEAN S.D. N Group: 4 C92711 C92713 C92714 C92716 C92719 C92722 C92726 C92728 C92741 C92748 MEAN S.D. N Dose Level: 2. 0 118 42 153 37 141 39 121 33 89 44 112 36 142 47 130 39 109 46 151 45 127 20.5 10 41 4. 7 10 Dose Level: 5.0 117 38 130 47 145 34 104 29 124 43 147 71 143 34 141 62 134 46 109 32 129 15.4 10 44 13. 6 10 Dosage Unit: ppm T-6295 0 0 3 3 1 0 0 0 2 0 1 1.3 10 10.5 10.6 10.3 10.6 10.5 10.3 10.5 10.0 10.6 10.2 10.4 .20 10 7.9 8.2 8.5 7.8 7.6 7.3 7.9 8.7 7.3 6.5 7.8 .64 10 Dosage Unit: ppm T-6295 2 10.4 0 11.0 0 10.6 0 10.4 0 10.5 0 10.3 1 10.2 0 10.9 1 10.0 5 10.3 7.7 7.1 7.8 7.5 8.3 7.1 7.0 8.4 7.2 6.7 1 1.6 10 10.5 .31 10 7.5 .57 10 146 146 147 147 147 146 147 147 146 152 147 1 .8 10 7.0 6.2 6.9 7.1 6.7 6.0 5.8 6.1 6.1 6.3 6.4 .46 10 146 153 146 144 146 147 144 150 145 147 147 2 .8 10 6.3 6.5 6.0 5.5 6.3 5.7 5.9 6.7 6.1 5.9 6.1 .37 10 104 102 103 103 105 106 105 104 105 110 105 2 10 104 108 101 104 104 103 102 102 102 105 104 2 10 Covance 6329-183 3M T-6295 1605 Appendix 5 Individual Clinical Chemistry Data Males Week 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER AST/SGOT ALT/SGPT IU/L IU/L GGT IU/L CA MG/DL I PHOS MG/DL NA MMOL/L K MMOL/L CL MMOL/L Group: 5 C92754 C92765 C92767 C92774 C92777 C92784 C92789 C92799 C92812 C92820 MEAN S.D. N Dose Level: 20.0 113 67 293 213 100 57 1 1 1 37 140 40 79 38 108 57 131 52 117 44 88 46 128 60. 7 10 65 52.9 10 Dosage Unit: ppm T-6295 0 10.2 0 10.6 5 10.7 1 10.6 0 10.1 0 10.5 0 10.4 0 10.7 0 10.8 0 10.6 8.1 8.1 7.8 8.3 7.1 7.3 6.8 7.9 8. 0 8.7 1 1.6 10 10.5 .23 10 7.8 .58 10 145 147 144 147 147 145 147 147 145 148 146 1.3 10 6.1 6.2 6.4 7.0 5.9 5.6 6.7 6.3 6.4 7.4 6.4 .52 10 102 104 104 103 105 104 105 102 102 106 104 1 10 Covance 6329-183 3M T-6295 1606 Appendix 5 Individual Clinical Chemistry Data Females Week 14 104 WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER GLU MG/DL UN MG/DL CREAT MG/DL T PRO G/DL ALB G/DL GLOB G/DL T BILI MG/DL CHOL MG/DL Group: 1 C92863 C92865 C92871 C92880 C92887 C92898 C92903 C92905 C92906 C92927 MEAN S.D. N Group: 2 C92935 C92940 C92944 C92948 C92956 C92962 C92967 C92978 C92986 C92987 MEAN S.D. N Dose Level: 0 109 14 108 16 113 16 112 13 112 14 113 14 94 16 77 15 103 19 117 16 106 12.0 10 15 1 .7 10 Dose Level: 0.5 107 13 113 15 88 15 106 17 109 9 112 21 110 15 92 12 117 15 105 16 106 9.1 10 15 3. 2 10 Dosage Unit: ppm T-6295 .7 7.6 5.5 .5 7.4 5.2 .8 8.4 5.8 .6 7.5 5.4 .6 8.0 5.9 .5 7.3 5.3 .8 7.3 5.3 .7 7.7 5.5 .5 7.6 5.8 .6 7.7 5.8 .6 7.6 5.6 .12 .34 .25 10 10 10 Dosage Unit: ppm T-6295 .6 8.4 6.5 .8 8. 1 5.9 .6 7.8 5.7 .8 8. 2 5.8 .4 8.0 6. 2 .7 7.5 5.7 .6 8.4 6.5 .5 8. 6 6. 6 .7 8. 2 6.0 .6 9.0 7.0 .6 8.2 6.2 .13 .42 .44 10 10 10 2.1 2.2 2.6 2.1 2.1 2.0 2.0 2.2 1.8 1.9 2.1 .22 10 1.9 2.2 2.1 2.4 1.8 1.8 1.9 2.0 2.2 2.0 2.0 .19 10 .0 89 .3 63 .0 63 .0 56 .2 72 . 1 107 .0 83 . 1 72 .6 70 . 1 71 . 1 75 .19 14 10 10 . 1 81 .4 69 . 1 65 .2 101 .3 63 .4 74 .0 80 . 1 98 .2 94 .3 155 .2 88 .14 27 10 10 Covance 6329-183 3M T-6295 1607 Appendix 5 Individual Clinical Chemistry Data Females Week 14 104 WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER GLU MG/DL UN MG/DL CREAT MG/DL T PRO G/DL ALB G/DL GLOB G/DL T BILI MG/DL CHOL MG/DL Group: 3 C92992 C92993 C93000 C93018 C93019 C93023 C93024 C93026 C93030 C93035 MEAN S.D. N Group: 4 C93051 C93064 C93071 C93076 C93078 C93085 C93089 C93090 C93093 C93109 MEAN S.D. N Dose Level: 2. 0 106 14 101 15 105 15 109 14 108 16 109 14 122 19 113 18 108 16 103 11 108 5.9 10 15 2 .3 10 Dose Level: 5. 0 85 19 103 15 87 16 96 12 92 14 85 17 105 13 108 14 96 16 91 15 95 8.3 10 15 2 .0 10 Dosage Unit: ppm T-6295 .7 .7 .8 .8 .7 .7 1.0 .9 .6 .9 .8 .12 10 7.8 7.7 7.8 7.8 7.9 7.5 8.3 8.5 7.7 8.1 7.9 .30 10 5.7 5.8 5.7 6.1 6.2 5.5 6.2 6.1 5.8 5.9 5.9 .24 10 Dosage Unit: ppm T-6295 .7 8.0 5.9 .8 8.7 6.4 .5 8.0 6.3 .6 7.8 5.3 .3 7.4 5.2 .6 7.8 6.0 .7 8. 6 6 . 1 .6 7.4 5.7 .5 7.9 5.5 .7 8.3 6.3 .6 8.0 5.9 .14 .44 .43 10 10 10 2.1 1.9 2.1 1.7 1.7 2.0 2.1 2.4 1.9 2.2 2.0 .22 10 2.1 2.3 1.7 2.5 2.2 1.8 2.5 1.7 2.4 2.0 2.1 .31 10 .2 71 .2 63 .0 63 . 1 113 . 1 106 .0 68 . 1 98 .0 131 . 1 71 .0 88 . 1 87 .08 23. 9 10 10 . 1 54 . 1 76 .2 94 . 1 71 .5 82 . 1 47 .2 62 . 1 70 .2 73 .4 70 .2 70 .14 13. 4 10 10 Covance 6329-183 3M T-6295 1608 Appendix 5 Individual Clinical Chemistry Data Females Week 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER GLU MG/DL UN MG/DL CREAT MG/DL T PRO G/DL ALB G/DL GLOB G/DL T BILI MG/DL CHOL MG/DL Group: 5 Dose Level: 20.0 Dosage Unit: ppm T-6295 C93111 C93113 C93120 C93127 C93135 C93143 C93155 C93162 C93169 C93178 104 14 .6 8.0 6.0 2. 0 .0 44 102 19 .6 7.6 5.9 1.7 .4 67 92 20 .7 7.9 6.0 1.9 . 1 45 87 15 .6 7.7 5.9 1 . 8 .2 69 94 18 .7 8.0 5.9 2 . 1 .3 76 104 18 .7 8. 6 6.4 2. 2 . 1 88 103 18 .6 7.4 5.8 1 . 6 .2 56 93 19 .6 8.0 6.0 2. 0 .2 73 102 17 .6 7.9 6. 2 1.7 .0 69 107 23 .7 8.9 6.9 2. 0 .4 75 MEAN S.D. N 99 18 .6 8.0 6 . 1 1.9 .2 66 6.7 2.5 .05 .45 .33 .19 .14 14.0 10 10 10 10 10 10 10 10 Covance 6329-183 3M T-6295 1609 Appendix 5 Individual Clinical Chemistry Data Females Week 14 104 WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER AST/SGOT ALT/SGPT IU/L IU/L GGT IU/L CA MG/DL I PHOS MG/DL NA MMOL/L K MMOL/L CL MMOL/L Group: 1 C92863 C92865 C92871 C92880 C92887 C92898 C92903 C92905 C92906 C92927 MEAN S.D. N Group: 2 C92935 C92940 C92944 C92948 C92956 C92962 C92967 C92978 C92986 C92987 MEAN S.D. N Dose Level: 0 89 29 110 35 115 36 100 37 94 33 77 31 133 34 113 34 107 36 88 33 103 16.3 10 34 2.4 10 Dose Level: 0. 5 87 28 132 36 140 32 113 23 95 28 97 35 108 55 88 34 100 43 113 41 107 17.8 10 36 9.1 10 Dosage Unit: ppm T-6295 3 2 3 0 0 1 0 0 1 1 1 1.2 10 11.0 10.3 10.7 10.6 10.6 11.7 10.1 10.3 10.7 10.8 10.7 .45 10 6.3 5.4 6.0 6.4 4.6 7.9 7.4 6.8 6.4 5.8 6.3 .95 10 Dosage Unit: ppm T-6295 4 11.3 0 10.7 0 10.4 3 11.1 1 10.5 0 11.0 0 11.0 1 11.3 4 11.3 0 12.1 7.4 7.2 6.1 7.9 4.5 5.9 4.0 6.1 7.4 7.1 1 1.7 10 11.1 .49 10 6.4 1.30 10 150 147 149 151 149 146 145 146 146 147 148 2.0 10 7.0 5.6 6.3 5.8 5.7 6.2 6.0 5.6 6.0 6.3 6.0 .43 10 146 149 146 149 147 149 147 147 153 149 148 2.1 10 6.9 6.7 6.1 7.4 5.5 5.9 5.9 5.5 7.5 7.1 6.4 .76 10 106 108 106 107 107 107 108 105 107 107 107 .9 10 103 107 105 105 106 108 106 105 108 104 106 1.6 10 Covance 6329-183 3M T-6295 1610 Appendix 5 Individual Clinical Chemistry Data Females Week 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER AST/SGOT ALT/SGPT IU/L IU/L GGT IU/L CA MG/DL I PHOS MG/DL NA MMOL/L K MMOL/L CL MMOL/L Group: 3 C92992 C92993 C93000 C93018 C93019 C93023 C93024 C93026 C93030 C93035 MEAN S.D. N Group: 4 C93051 C93064 C93071 C93076 C93078 C93085 C93089 C93090 C93093 C93109 MEAN S.D. N Dose Level: 2. 0 81 31 100 25 108 25 93 40 174 85 93 33 115 40 93 28 85 25 90 35 103 26.9 10 37 17.9 10 Dose Level: 5.0 144 37 92 37 105 36 90 34 90 26 91 29 101 35 102 37 100 30 115 42 103 16.5 10 34 4.7 10 Dosage Unit: ppm T-6295 0 0 0 0 3 0 1 1 4 0 1 1.4 10 10.6 10.5 10.5 11.3 11.6 10.5 11.1 11.5 10.8 10.7 10.9 .43 10 6.4 5.3 6.7 6.6 7.4 5.7 7.7 7.6 6.6 5.4 6.5 .87 10 Dosage Unit: ppm T-6295 0 10.7 0 11.3 2 10.7 5 10.3 0 10.4 1 10.4 1 11.3 4 9.8 4 10.7 0 11.4 8.1 6.5 7.4 6.6 6.3 8.2 6.6 4.5 6.5 5.3 2 1.9 10 10.7 .51 10 6.6 1.14 10 148 145 146 147 149 148 155 148 145 145 148 3.0 10 6.4 5.5 5.1 6.3 7.0 6.1 7.1 6.8 6.1 5.2 6.2 .71 10 150 150 147 145 148 145 149 146 144 153 148 2.8 10 6.3 6.6 5.2 6.1 6.4 4.6 6.3 4.7 6.1 5.9 5.8 .72 10 106 106 104 103 107 107 107 105 105 104 105 1 10 108 108 106 106 108 104 107 106 104 110 107 1 10 Covance 6329-183 3M T-6295 1611 Appendix 5 Individual Clinical Chemistry Data Females Week 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER AST/SGOT ALT/SGPT IU/L IU/L GGT IU/L CA MG/DL I PHOS MG/DL NA MMOL/L K MMOL/L CL MMOL/L Group: 5 C93111 C93113 C93120 C93127 C93135 C93143 C93155 C93162 C93169 C93178 MEAN S.D. N Dose Level: 20.0 66 30 92 43 85 33 79 28 137 89 85 40 77 35 8880 27 31 115 37 2900.7 10 39 18.2 10 Dosage Unit: ppm T-6295 1 11.0 110 10.6 10.4 10.4 41 1101..25 00 1100..87 0 4 1101..27 76..81 76..10 66..27 5.5 7.3 5.0 7.2 1 10.8 1.5 .30 10 10 6.5 .77 10 148 149 146 145 147 153 151 145 148 150 1482.6 10 666...228 5.1 56..66 6.2 56..08 7.0 6.2 .57 10 106 106 106 104 111006 111 102 110 104 106 3.0 10 Covance 6329-183 3M T-6295 1612 Appendix 5 Individual Clinical Chemistry Data Males Week 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER PCOAO IU/G Group: 1 C92509 C92511 C92521 C92528 C92532 MEAN S.D. N Group: 2 C92593 C92600 C92616 C92621 C92627 MEAN S.D. N Group: 3 C92640 C92645 C92662 C92676 C92684 MEAN S.D. N Dose Level: 0 4 6 3 6 4 5 1.3 5 Dose Level: 0.5 8 1 8 5 2 5 3.3 5 Dose Level: 2.0 8 4 8 1 6 5 3.0 5 Dosage Unit: ppm T-6295 Dosage Unit: ppm T-6295 Dosage Unit: ppm T-6295 Covance 6329-183 3M T-6295 1613 Appendix 5 Individual Clinical Chemistry Data Males Week 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER PCOAO IU/G Group: 4 C92713 C92714 C92719 C92722 C92728 MEAN S.D. N Group: 5 C92765 C92777 C92789 C92799 C92812 MEAN S.D. N Dose Level: 5.0 2 0 4 3 0 2 1.8 5 Dose Level: 20.0 4 3 8 6 6 5 1.9 5 Dosage Unit: ppm T-6295 Dosage Unit: ppm T-6295 Covance 6329-183 3M T-6295 1614 Appendix 5 Individual Clinical Chemistry Data Females Week 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER PCOAO IU/G Group: 1 C92880 C92887 C92898 C92903 C92905 MEAN S.D. N Group: 2 C92944 C92962 C92967 C92978 C92987 MEAN S.D. N Group: 3 C92993 C93000 C93018 C93023 C93035 MEAN S.D. N Dose Level: 0 0 3 4 1 1 2 1.6 5 Dose Level: 0.5 2 4 6 0 1 3 2.4 5 Dose Level: 2.0 2 1 1 0 1 1 .7 5 Dosage Unit: ppm T-6295 Dosage Unit: ppm T-6295 Dosage Unit: ppm T-6295 Covance 6329-183 3M T-6295 1615 Appendix 5 Individual Clinical Chemistry Data Females Week 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER PCOAO IU/G Group: 4 C93051 C93064 C93071 C93085 C93109 MEAN S.D. N Group: 5 C93111 C93127 C93143 C93155 C93169 MEAN S.D. N Dose Level: 5.0 0 0 6 2 0 2 2.6 5 Dose Level: 20.0 5 7 6 0 7 5 2.9 5 Dosage Unit: ppm T-6295 Dosage Unit: ppm T-6295 Covance 6329-183 3M T-6295 1616 Appendix 5 Individual Clinical Chemistry Data Males Week 27 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER GLU MG/DL UN MG/DL CREAT MG/DL T PRO G/DL ALB G/DL GLOB G/DL T BILI MG/DL CHOL MG/DL Group: 1 C92505 C92510 C92512 C92525 C92537 C92543 C92545 C92563 C92567 C92570 MEAN S.D. N Group: 2 C92577 C92583 C92585 C92586 C92588 C92590 C92601 C92602 C92617 C92630 MEAN S.D. N Dose Level: 0 107 16 125 11 119 12 126 16 120 14 105 11 107 10 121 10 108 11 111 11 115 8.1 10 12 2.3 10 Dose Level: 0.5 126 15 103 12 125 15 113 10 102 12 131 12 93 11 118 12 141 14 119 14 117 14.7 10 13 1.7 10 Dosage Unit: ppm T-6295 .6 6.5 4.4 .8 6. 8 4.6 .6 7.8 4.9 .7 7.4 4.8 .8 7.1 5.3 .9 7.3 4.9 .7 7.1 4.8 .7 7.8 5.1 .6 7.7 5.2 .8 7.4 4.6 .7 7.3 4.9 .10 .43 .28 10 10 10 Dosage Unit: ppm T-6295 .7 7.0 4.9 .8 7.1 4.8 .8 6. 6 4.3 .6 7.2 5.1 .8 6.7 4.4 .8 7.2 4.8 .7 6.5 4.4 .7 7.4 5.1 1 . 1 8.2 5.0 .5 7.2 4.6 .8 7.1 4.7 .16 .48 .30 10 10 10 2.1 2.2 2.9 2.6 1.8 2.4 2.3 2.7 2.5 2.8 2.4 .34 10 2.1 2.3 2.3 2.1 2.3 2.4 2.1 2.3 3.2 2.6 2.4 .33 10 .2 55 . 1 70 . 1 91 .3 82 .4 110 .3 87 .2 66 .4 131 .2 120 . 1 74 .2 89 .12 24.7 10 10 .3 74 .4 63 .3 93 .3 55 .3 86 .3 76 .2 67 .4 50 .3 148 .3 129 .3 84 .06 31.8 10 10 Covance 6329-183 3M T-6295 1617 Appendix 5 Individual Clinical Chemistry Data Males Week 27 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER GLU MG/DL UN MG/DL CREAT MG/DL T PRO G/DL ALB G/DL GLOB G/DL T BILI MG/DL CHOL MG/DL Group: 3 C92633 C92644 C92647 C92658 C92660 C92667 C92673 C92679 C92686 C92687 MEAN S.D. N Group: 4 C92696 C92698 C92708 C92709 C92710 C92725 C92743 C92745 C92749 C92750 MEAN S.D. N Dose Level: 2. 0 129 13 103 18 115 14 119 12 97 12 136 13 104 10 103 10 110 14 128 12 114 13.2 10 13 2 .3 10 Dose Level: 5.0 98 14 91 11 113 15 118 10 116 19 109 15 121 13 124 12 110 14 130 13 113 11.7 10 14 2 .5 10 Dosage Unit: ppm T-6295 .9 7.4 4.6 .7 6.9 4.7 .8 7.2 5.0 .7 7.4 4.9 .8 7.2 4.7 .8 7.5 4.7 .6 7.2 4.8 .8 6.8 4.7 .7 6.9 4.8 .7 7.7 5.4 .8 7.2 4.8 .08 .29 .23 10 10 10 Dosage Unit: ppm T-6295 .8 7.5 5.1 .6 7.0 4.7 .7 7.6 4.9 .5 7.2 4.6 1. 0 7.2 4.7 .8 7.0 4.8 .9 8. 2 5.5 .5 7.5 4.9 .7 6.7 4.7 .8 7.9 5.2 .7 7.4 4.9 .16 .45 .28 10 10 10 2.8 2.2 2.2 2.5 2.5 2.8 2.4 2.1 2.1 2.3 2.4 .26 10 2.4 2.3 2.7 2.6 2.5 2.2 2.7 2.6 2.0 2.7 2.5 .24 10 .2 89 .5 44 .4 63 .6 86 .2 70 .4 84 .5 86 .5 65 .2 64 .2 92 .4 74 .16 15 10 10 .3 47 .4 47 .2 60 . 1 139 .2 67 . 1 71 .4 81 .4 80 .3 43 .3 89 .3 72 .12 28 10 10 Covance 6329-183 3M T-6295 1618 Appendix 5 Individual Clinical Chemistry Data Males Week 27 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER GLU MG/DL UN MG/DL CREAT MG/DL T PRO G/DL ALB G/DL GLOB G/DL T BILI MG/DL CHOL MG/DL Group: 5 Dose Level : 20.0 Dosage Unit: ppm T-6295 C92756 C92761 C92764 C92773 C92783 C92788 C92791 C92803 C92804 C92820 120 17 .7 7.2 4.9 2.3 . 1 46 86 19 1 . 1 8. 1 5.5 2. 6 .2 36 112 19 .6 7.2 4.7 2.5 .2 38 103 17 .7 7.8 5.2 2. 6 . 1 28 117 13 .7 7.8 5.0 2. 8 .4 65 118 16 .9 7.1 4.9 2. 2 . 1 45 114 19 .8 7.9 5.5 2.4 .3 61 114 20 .9 7.5 5.1 2.4 .3 44 132 16 .9 7.3 5.1 2. 2 .3 57 98 16 .9 7.5 5.1 2.4 .2 43 MEAN S.D. N 1 1 1 17 .8 7.5 5.1 2.4 .2 46 12. 8 2 ..1 .15 .34 .25 .19 .10 1 1 . 6 10 10 10 10 10 10 10 10 Covance 6329-183 3M T-6295 1619 Appendix 5 Individual Clinical Chemistry Data Males Week 27 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER AST/SGOT ALT/SGPT IU/L IU/L GGT IU/L CA MG/DL I PHOS MG/DL NA MMOL/L K MMOL/L CL MMOL/L Group: 1 C92505 C92510 C92512 C92525 C92537 C92543 C92545 C92563 C92567 C92570 MEAN S.D. N Group: 2 C92577 C92583 C92585 C92586 C92588 C92590 C92601 C92602 C92617 C92630 MEAN S.D. N Dose Level: 0 80 33 61 40 101 34 110 43 74 25 74 33 108 40 75 40 64 33 94 46 84 17.8 10 37 6 .2 10 Dose Level: 0.5 66 34 79 42 85 31 83 35 77 41 80 28 64 33 81 43 81 30 112 45 81 13.0 10 36 6 .1 10 Dosage Unit: ppm T-6295 0 2 0 0 1 2 1 3 4 1 1 1.3 10 10.6 10.7 11.4 11.1 11.1 10.9 10.7 11.4 11.7 11.2 11.1 .36 10 6.8 6.8 6.9 7.0 6.9 6.4 7.0 5.9 6.4 6.9 6.7 .36 10 Dosage Unit: ppm T-6295 0 11.3 1 11.0 4 10.5 4 10.8 3 11.1 1 10.9 0 10.8 0 11.4 4 12.0 3 11.5 7.0 7.3 5.8 6.5 6.7 6.6 7.0 6.5 7.7 7.7 2 1.8 10 11.1 .43 10 6.9 .59 10 146 148 146 145 144 146 147 146 147 146 146 1 .1 10 6.2 6.7 7.0 6.9 6.7 6.4 6.6 6.7 6.6 6.8 6.7 .23 10 148 145 143 147 146 146 146 149 148 148 147 1 .8 10 6.8 6.8 6.7 7.0 6.4 7.0 6.5 7.3 7.8 8. 0 7.0 .53 10 105 107 104 101 105 105 104 101 106 103 104 2 10 106 104 103 105 105 103 105 107 103 103 104 1 10 Covance 6329-183 3M T-6295 1620 Appendix 5 Individual Clinical Chemistry Data Males Week 27 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER AST/SGOT ALT/SGPT IU/L IU/L GGT IU/L CA MG/DL I PHOS MG/DL NA MMOL/L K MMOL/L CL MMOL/L Group: 3 C92633 C92644 C92647 C92658 C92660 C92667 C92673 C92679 C92686 C92687 MEAN S.D. N Group: 4 C92696 C92698 C92708 C92709 C92710 C92725 C92743 C92745 C92749 C92750 MEAN S.D. N Dose Level: 2. 0 71 39 124 37 70 53 103 60 104 44 65 40 128 54 88 42 88 34 82 65 92 22.0 10 47 1 0 .5 10 Dose Level: 5.0 85 42 92 36 89 47 71 30 92 41 82 34 76 45 69 34 83 28 65 40 80 9.7 10 38 6 .3 10 Dosage Unit: ppm T-6295 1 1 1 1 0 2 1 1 0 0 1 .6 10 11.4 10.8 11.0 11.3 10.9 11.5 10.8 10.9 10.9 11.4 11.1 .28 10 7.0 7.2 6.7 7.3 7.0 6.7 6.4 6.8 6.8 6.4 6.8 .30 10 Dosage Unit: ppm T-6295 1 11.1 0 10.8 0 11.6 1 11.8 0 11.0 3 11.3 4 12.2 0 10.9 2 10.3 1 11.8 6.8 6.8 8.3 6.9 7.1 6.6 8.2 6.8 6.6 6.9 1 1.4 10 11.3 .57 10 7.1 .62 10 148 149 146 146 147 150 147 144 145 145 147 1 .9 10 7.1 7.3 6.3 7.2 7.3 7.1 6.4 6.3 5.8 6.3 6.7 .54 10 149 148 150 146 147 147 151 146 145 149 148 1 .9 10 6.7 6.7 7.5 7.0 6.8 7.0 7.5 6.4 6.0 7.1 6.9 .46 10 104 107 106 104 103 105 104 102 104 103 104 1 10 106 104 105 104 106 106 103 102 104 105 104 1 10 Covance 6329-183 3M T-6295 1621 Appendix 5 Individual Clinical Chemistry Data Males Week 27 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER AST/SGOT ALT/SGPT IU/L IU/L GGT IU/L CA MG/DL I PHOS MG/DL NA MMOL/L K MMOL/L CL MMOL/L Group: 5 C92756 C92761 C92764 C92773 C92783 C92788 C92791 C92803 C92804 C92820 MEAN S.D. N Dose Level: 20.0 82 37 139 64 127 75 72 37 63 38 233 194 61 44 108 83 62 37 79 38 103 53. 6 10 65 48.7 10 Dosage Unit: ppm T-6295 2 10.7 0 11.7 1 11.1 0 11.0 1 11.6 6 10.9 0 11.0 0 11.6 1 11.0 3 11.3 7.3 7.9 7.7 6.8 6.7 7.6 5.4 8. 6 6.6 8.1 1 1.9 10 11.2 .34 10 7.3 .92 10 145 149 147 146 145 146 144 152 144 148 147 2 ..5 10 6.5 7.7 7.5 6.9 6.4 6.5 6.0 7.8 6.2 7.2 6.9 .65 10 103 105 105 104 105 106 102 107 104 104 104 1 10 Covance 6329-183 3M T-6295 1622 Appendix 5 Individual Clinical Chemistry Data Females Week 27 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER GLU MG/DL UN MG/DL CREAT MG/DL T PRO G/DL ALB G/DL GLOB G/DL T BILI MG/DL CHOL MG/DL Group: 1 C92876 C92878 C92888 C92906 C92916 C92917 C92921 C92922 C92923 C92928 MEAN S.D. N Group: 2 C92932 C92935 C92938 C92951 C92954 C92956 C92960 C92974 C92979 C92989 MEAN S.D. N Dose Level: 0 92 15 115 12 101 13 104 15 97 16 107 14 102 12 114 14 99 14 108 14 104 7.3 10 14 1.3 10 Dose Level: 0.5 89 18 104 12 89 15 102 17 101 16 106 13 99 16 93 12 92 14 101 13 98 6.3 10 15 2.1 10 Dosage Unit: ppm T-6295 1 . 1 8.8 6.4 1 . 1 8.7 6.4 .8 8.9 6.3 .7 8.7 6.4 1 . 1 8.3 5.6 .9 9.6 6.8 .8 8.6 6. 1 .9 9.3 7.1 .9 8.9 6.4 .6 9.1 6.8 .9 8.9 6.4 .17 .37 .41 10 10 10 Dosage Unit: ppm T-6295 1 . 1 7.9 6. 2 .6 9.3 7.1 1. 0 9.0 6. 2 .9 7.4 5.3 .9 7.7 5.4 .7 8.9 6.8 1 . 2 8.0 5.8 1 . 1 8. 2 5.8 .7 8.9 6. 6 1 . 1 9.0 6.5 .9 8.4 6. 2 . 21 .66 .59 10 10 10 2.4 2.3 2.6 2.3 2.7 2.8 2.5 2.2 2.5 2.3 2.5 .20 10 1.7 2.2 2.8 2.1 2.3 2.1 2.2 2.4 2.3 2.5 2.3 .29 10 .4 139 . 1 82 .5 79 .2 100 .3 149 .3 128 .4 129 .4 133 .4 129 .6 86 .4 115 .14 26 10 10 .2 137 .4 80 .4 110 .3 73 .4 92 .1 68 .3 82 .3 72 .4 87 .3 153 .3 95 .10 29 10 10 Covance 6329-183 3M T-6295 1623 Appendix 5 Individual Clinical Chemistry Data Females Week 27 104 WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER GLU MG/DL UN MG/DL CREAT MG/DL T PRO G/DL ALB G/DL GLOB G/DL T BILI MG/DL CHOL MG/DL Group: 3 C93003 C93004 C93011 C93020 C93027 C93030 C93034 C93046 C93047 C93049 MEAN S.D. N Group: 4 C93052 C93055 C93072 C93080 C93081 C93090 C93094 C93095 C93103 C93104 MEAN S.D. N Dose Level: 2.0 99 14 93 15 92 11 97 14 102 17 100 18 108 16 108 15 106 14 82 16 99 8.2 10 15 1.9 10 Dose Level: 5.0 112 14 86 20 99 15 111 11 89 14 94 17 96 17 88 14 98 17 112 9 98 10.0 10 15 3.2 10 Dosage Unit: ppm T-6295 .8 .9 1.0 .9 1.2 1.1 1.1 1.1 .9 .7 1.0 .16 10 7.7 8. 0 9.0 8.1 8.4 9.9 8.9 8.5 7.9 7.6 8.4 .71 10 5.2 5.4 6.2 5.6 5.9 7.2 6.9 6.1 5.8 5.4 6.0 .65 10 Dosage Unit: ppm T-6295 1 . 1 8.4 6. 2 .8 8.4 6.3 1 . 1 8.5 6.0 .9 8. 6 6. 2 1. 0 8. 2 5.7 .9 8. 2 6 . 1 .7 7.9 5.7 .7 8. 2 6.0 .9 7.4 5.4 1. 0 8.8 5.8 .9 8.3 5.9 .14 .39 .28 10 10 10 2.5 2.6 2.8 2.5 2.5 2.7 2.0 2.4 2.1 2.2 2.4 .26 10 2.2 2.1 2.5 2.4 2.5 2.1 2.2 2.2 2.0 3.0 2.3 .29 10 . 1 60 .5 70 .2 82 .4 56 . 1 87 .3 94 .4 107 .2 76 .2 64 .2 89 .3 78 .13 16 10 10 . 1 84 . 1 63 .2 80 .2 86 .3 68 .3 76 .4 72 .2 70 .2 95 .6 94 .3 79 .15 10 10 10 Covance 6329-183 3M T-6295 1624 Appendix 5 Individual Clinical Chemistry Data Females Week 27 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER GLU MG/DL UN MG/DL CREAT MG/DL T PRO G/DL ALB G/DL GLOB G/DL T BILI MG/DL CHOL MG/DL Group: 5 C93119 C93131 C93133 C93138 C93149 C93153 C93157 C93160 C93162 C93179 MEAN S.D. N Dose Level: 20.0 100 22 89 16 89 19 92 94 10906 2107 21 19 104 18 98 81 2206 946.8 202.8 10 10 Dosage Unit: ppm T-6295 1.0 9.2 6.8 1..07 .7 .7 ..89 9.5 88..15 89..10 8.1 666...605 6.6 6.0 6.0 1..09 .9 9.0 9.4 8.4 6.4 76..21 .9 8.7 6.4 .13 .55 .37 10 10 10 2.4 3.0 21..19 22..14 2.1 2.6 22..23 2.3 .31 10 ..65 75 90 .4 73 ..24 55 72 ..24 .2 .2 75 70 145 70 .4 75 .4 80 .14 24.4 10 10 Covance 6329-183 3M T-6295 1625 Appendix 5 Individual Clinical Chemistry Data Females Week 27 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER AST/SGOT ALT/SGPT IU/L IU/L GGT IU/L CA MG/DL I PHOS MG/DL NA MMOL/L K MMOL/L CL MMOL/L Group: 1 C92876 C92878 C92888 C92906 C92916 C92917 C92921 C92922 C92923 C92928 MEAN S.D. N Group: 2 C92932 C92935 C92938 C92951 C92954 C92956 C92960 C92974 C92979 C92989 MEAN S.D. N Dose Level: 0 321 194 106 49 113 96 82 32 94 36 84 44 92 36 102 49 107 67 85 39 119 71.9 10 64 49. 4 10 Dose Level: 0.5 128 31 79 30 104 30 97 27 85 34 79 34 97 28 130 26 118 54 102 41 102 18.6 10 34 8 .4 10 Dosage Unit: ppm T-6295 3 12.0 0 11.2 5 12.0 4 11.3 0 12.1 0 12.1 1 11.9 4 11.4 1 11.8 0 11.6 7.5 6.4 8.1 5.4 6.8 6.0 7.1 6.2 6.6 6.8 2 2.0 10 11.7 .34 10 6.7 .77 10 Dosage Unit: ppm T-6295 0 12.7 1 11.8 4 11.7 3 10.6 1 11.4 0 11.9 2 11.2 1 11.1 1 12.7 4 11.5 5.7 6.8 7.9 6.4 6.2 6.3 5.8 7.2 7.1 5.6 2 1.5 10 11.7 .67 10 6.5 .74 10 146 148 150 145 149 145 148 147 150 151 148 2.1 10 6.3 5.3 6.9 4.8 6.5 5.5 6.1 4.8 6.2 6.0 5.8 .71 10 145 146 152 147 144 147 146 147 145 148 147 2.2 10 4.9 6.9 5.8 5.5 5.9 6.1 5.9 6.6 6.5 4.8 5.9 .69 10 103 109 106 106 104 104 105 104 105 110 106 2 10 107 104 106 111 105 105 107 107 105 106 106 1 10 Covance 6329-183 3M T-6295 1626 Appendix 5 Individual Clinical Chemistry Data Females Week 27 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER AST/SGOT ALT/SGPT IU/L IU/L GGT IU/L CA MG/DL I PHOS MG/DL NA MMOL/L K MMOL/L CL MMOL/L Group: 3 C93003 C93004 C93011 C93020 C93027 C93030 C93034 C93046 C93047 C93049 MEAN S.D. N Group: 4 C93052 C93055 C93072 C93080 C93081 C93090 C93094 C93095 C93103 C93104 MEAN S.D. N Dose Level: 2. 0 100 30 468 448 77 30 97 33 104 54 87 32 130 49 110 33 160 29 93 34 143 116.7 10 77 130. 6 10 Dose Level: 5.0 97 28 105 36 86 34 96 46 104 33 100 40 185 115 85 33 195 63 119 42 117 39.6 10 47 25. 8 10 Dosage Unit: ppm T-6295 3 6 3 1 1 1 0 4 0 0 2 2.0 10 10.9 10.9 11.4 10.9 11.8 11.9 11.2 11.0 11.0 10.9 11.2 .38 10 6.9 8. 0 5.6 6.0 7.7 6.0 4.8 6.0 6.8 6.5 6.4 .96 10 Dosage Unit: ppm T-6295 4 11.9 4 11.3 3 11.7 4 11.0 3 11.1 4 10.8 7 11.4 4 11.0 4 10.8 0 11.3 7.2 6.4 6.6 5.7 8.2 6.9 6.9 7.2 5.7 6.4 4 1.7 10 11.2 .37 10 6.7 .75 10 146 146 148 147 154 148 147 149 147 146 148 2.4 10 6.1 5.6 5.9 5.3 7.1 5.4 5.1 5.7 6.2 5.9 5.8 .57 10 151 146 149 148 149 145 145 148 147 147 148 1.9 10 7.0 6.1 6.9 5.9 6.2 5.1 6.0 5.9 5.5 5.2 6.0 .63 10 107 105 107 110 110 107 105 108 107 106 107 1 10 112 107 109 108 108 106 106 109 108 105 108 2 10 Covance 6329-183 3M T-6295 1627 Appendix 5 Individual Clinical Chemistry Data Females Week 27 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER AST/SGOT ALT/SGPT IU/L IU/L GGT IU/L CA MG/DL I PHOS MG/DL NA MMOL/L K MMOL/L CL MMOL/L Group: 5 C93119 C93131 C93133 C93138 C93149 C93153 C93157 C93160 C93162 C93179 MEAN S.D. N Dose Level: 20.0 79 36 167 130 116 30 60 24 97 58 83 32 95 42 274 196 80 37 115 51 117 62. 6 10 64 55.5 10 Dosage Unit: ppm T-6295 0 11.3 6.9 1 12.0 6.7 0 11.3 8.2 0 11.1 6.3 2 11.3 7.0 0 10.9 1 11.0 6.5 6.9 7 11.7 6.5 4 11.8 7.4 6 12.2 10.3 2 2.6 10 11.5 .44 10 7.3 1.20 10 150 146 147 145 153 147 149 144 147 152 148 2 .9 10 6.2 6.0 5.9 5.3 5.8 5.6 5.3 5.5 6.8 7.4 6.0 .67 10 107 105 106 105 109 108 109 103 105 108 106 2.0 10 Covance 6329-183 3M T-6295 1628 Appendix 5 Individual Clinical Chemistry Data Males Week 53 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER GLU MG/DL UN MG/DL CREAT MG/DL T PRO G/DL ALB G/DL GLOB G/DL T BILI MG/DL CHOL MG/DL Group: 1 C92501 C92504 C92507 C92512 C92517 C92523 C92534 C92540 C92562 C92570 MEAN S.D. N Group: 2 C92573 C92587 C92592 C92596 C92604 C92612 C92614 C92624 C92625 C92629 MEAN S.D. N Dose Level: 0 123 117 127 127 154 137 128 147 116 115 129 13.2 10 11 9 11 11 10 12 8 12 9 9 10 1 .4 10 Dose Level: 0.5 120 12 113 12 139 11 129 11 117 13 121 12 106 11 126 10 133 14 148 11 125 12.5 10 12 1 .2 10 Dosage Unit: ppm T-6295 .8 7.3 4.6 .7 6.8 4.2 .4 7.5 5.0 .4 7.0 4.1 .8 7.3 4.6 .6 6.8 4.5 .5 6.7 3.8 .9 7.8 4.7 .7 6. 6 4.5 .6 6.3 4.1 .6 7.0 4.4 .17 .46 .35 10 10 10 Dosage Unit: ppm T-6295 .7 7.4 4.7 .7 7.6 4.5 .8 7.3 5.0 .6 8.0 5.4 .7 7.4 4.8 .7 7.4 4.7 .6 7.1 4.8 .8 7.4 4.9 .9 7.7 4.6 .7 7.2 4.6 .7 7.4 4.8 .09 .26 .26 10 10 10 2.7 2.6 2.5 2.9 2.7 2.3 2.9 3.1 2.1 2.2 2.6 .33 10 2.7 3.1 2.3 2.6 2.6 2.7 2.3 2.5 3.1 2.6 2.6 .28 10 .0 226 .0 85 .0 133 .0 110 . 1 143 .0 79 .0 92 .0 146 .2 99 .0 88 .0 120 .07 44 10 10 .0 92 .1 201 . 1 108 .0 94 .3 78 .3 76 .0 102 . 1 139 .2 187 .5 216 .2 129 .16 53 10 10 Covance 6329-183 3M T-6295 1629 Appendix 5 Individual Clinical Chemistry Data Males Week 53 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER GLU MG/DL UN MG/DL CREAT MG/DL T PRO G/DL ALB G/DL GLOB G/DL T BILI MG/DL CHOL MG/DL Group: 3 C92634 C92635 C92643 C92649 C92653 C92654 C92667 C92669 C92680 C92686 MEAN S.D. N Group: 4 C92694 C92704 C92710 C92711 C92727 C92729 C92739 C92741 C92742 C92748 MEAN S.D. N Dose Level: 2. 0 108 136 107 104 137 118 140 111 121 132 121 13.8 10 13 13 10 10 13 12 12 14 10 13 12 1 .5 10 Dose Level: 5. 0 116 16 122 14 117 16 126 14 130 14 130 12 115 13 107 13 131 13 118 15 121 8. 0 10 14 1 .3 10 Dosage Unit: ppm T-6295 .6 7.0 4.5 .8 7.4 5.0 .6 7.3 4.7 .5 6. 6 4.5 .7 7.6 4.8 .8 7.0 4.5 .9 8.0 4.8 .8 7.1 4.9 .4 7.4 4.6 .7 7.4 5.0 .7 7.3 4.7 .15 .38 .20 10 10 10 Dosage Unit: ppm T-6295 .5 7.5 5.1 .7 7.5 4.8 .8 7.4 4.7 .8 7.1 4.6 .9 8.4 5.2 .6 7.0 4.2 .8 8. 2 5.0 .5 7.3 4.9 1. 0 7.3 4.9 .8 7.9 4.9 .7 7.6 4.8 .16 .46 .28 10 10 10 2.5 2.4 2.6 2.1 2.8 2.5 3.2 2.2 2.8 2.4 2.6 .32 10 2.4 2.7 2.7 2.5 3.2 2.8 3.2 2.4 2.4 3.0 2.7 .32 10 .0 94 .0 85 . 1 94 .0 62 .2 94 .2 61 .1 111 .0 98 .0 111 .2 76 . 1 89 .09 17.7 10 10 . 1 78 .3 85 . 1 78 .0 82 . 1 106 .0 90 .0 101 .0 101 .3 75 .1 122 . 1 92 .12 15.2 10 10 Covance 6329-183 3M T-6295 1630 Appendix 5 Individual Clinical Chemistry Data Males Week 53 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER GLU MG/DL UN MG/DL CREAT MG/DL T PRO G/DL ALB G/DL GLOB G/DL T BILI MG/DL CHOL MG/DL Group: 5 Dose Level : 20.0 Dosage Unit: ppm T-6295 C92751 C92754 C92755 C92778 C92790 C92795 C92796 C92817 C92820 109 13 .8 6.8 4.8 2. 0 .0 24 108 14 .6 7.5 4.9 2. 6 .0 47 94 17 .6 7.8 5.4 2.4 .0 74 116 9 .7 6.7 4.3 2.4 .0 61 117 12 .8 7.8 5.0 2. 8 .0 69 107 15 .6 7.0 4.7 2.3 . 1 46 127 11 .8 6.0 3.5 2.5 .0 24 115 13 .6 6.5 4.3 2. 2 .0 59 97 14 .7 7.3 4.9 2.4 .0 46 MEAN S.D. N 110 13 .7 7.0 4.6 2.4 .0 50 10. 2 2.3 .09 .61 .55 .23 .03 17.8 99999999 Covance 6329-183 3M T-6295 1631 Appendix 5 Individual Clinical Chemistry Data Males Week 53 104 WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER AST/SGOT ALT/SGPT IU/L IU/L GGT IU/L CA MG/DL I PHOS MG/DL NA MMOL/L K MMOL/L CL MMOL/L Group: 1 C92501 C92504 C92507 C92512 C92517 C92523 C92534 C92540 C92562 C92570 MEAN S.D. N Group: 2 C92573 C92587 C92592 C92596 C92604 C92612 C92614 C92624 C92625 C92629 MEAN S.D. N Dose Level: 0 63 26 92 35 108 38 131 32 97 29 1 1 1 38 101 26 154 241 127 41 74 37 106 27.0 10 54 65.8 10 Dose Level: 0. 5 125 58 177 58 124 70 99 47 177 205 102 30 82 45 143 41 81 26 67 44 118 38.8 10 62 51.8 10 Dosage Unit: ppm T-6295 0 0 0 0 0 0 0 0 0 0 0 .0 10 11.1 9.9 10.9 10.2 10.9 10.3 10.3 10.7 10.3 10.2 10.5 .39 10 6.9 6.0 6.5 6.1 6.2 6.7 6.3 5.8 5.9 7.0 6.3 .42 10 Dosage Unit: ppm T-6295 0 11.1 0 11.4 1 11.1 0 11.4 0 11.1 1 11.3 0 11.3 0 10.8 1 12.2 0 11.9 6.9 7.8 6.6 7.0 7.3 7.3 6.9 6.8 7.2 7.2 0 .5 10 11.4 .41 10 7.1 .34 10 150 146 148 144 147 147 145 146 146 147 147 1.6 10 5.8 5.3 6.1 5.8 6.3 6.2 6.7 6.0 5.5 5.4 5.9 .44 10 149 150 149 150 148 145 151 143 149 149 148 2.5 10 6.5 7.5 6.7 6.9 5.8 5.8 7.0 6.0 6.5 6.4 6.5 .55 10 106 107 108 105 105 108 105 103 106 109 106 1.8 10 105 107 106 106 107 108 110 103 105 106 106 1.9 10 Covance 6329-183 3M T-6295 1632 Appendix 5 Individual Clinical Chemistry Data Males Week 53 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER AST/SGOT ALT/SGPT IU/L IU/L GGT IU/L CA MG/DL I PHOS MG/DL NA MMOL/L K MMOL/L CL MMOL/L Group: 3 C92634 C92635 C92643 C92649 C92653 C92654 C92667 C92669 C92680 C92686 MEAN S.D. N Group: 4 C92694 C92704 C92710 C92711 C92727 C92729 C92739 C92741 C92742 C92748 MEAN S.D. N Dose Level: 2. 0 105 134 91 119 101 107 94 143 107 112 111 16.6 10 57 42 30 43 38 34 40 43 33 42 40 7. 5 10 Dose Level: 5.0 119 39 167 40 125 44 166 54 104 55 116 36 118 49 113 51 144 34 130 33 130 21.9 10 44 8 .3 10 Dosage Unit: ppm T-6295 0 2 0 1 2 0 0 0 0 0 0 .8 10 10.7 11.4 11.1 10.3 11.4 11.2 11.4 10.4 10.7 11.3 11.0 .43 10 7.1 7.4 7.3 7.0 8. 0 8. 0 7.5 6.2 6.4 7.2 7.2 .59 10 Dosage Unit: ppm T-6295 4 11.3 0 11.5 0 11.1 1 10.6 0 11.9 0 10.4 0 11.6 0 10.5 0 10.7 0 11.7 8. 6 8.1 7.9 7.2 8.3 7.5 7.6 6.5 7.3 8.4 0 1.3 10 11.1 .55 10 7.7 .64 10 150 150 149 149 151 151 153 148 146 145 149 2 .4 10 6.5 6.9 6.8 6.3 7.3 7.2 6.9 5.9 6.0 5.7 6.6 .56 10 153 148 147 146 155 146 150 148 145 153 149 3. 5 10 7.5 6.8 6.6 7.0 7.5 6.7 7.1 5.9 5.6 7.2 6.8 .63 10 109 108 105 110 107 109 105 107 108 106 107 1 10 112 107 106 110 107 107 109 106 104 105 107 2 10 Covance 6329-183 3M T-6295 1633 Appendix 5 Individual Clinical Chemistry Data Males Week 53 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER AST/SGOT ALT/SGPT IU/L IU/L GGT IU/L CA MG/DL I PHOS MG/DL NA MMOL/L K MMOL/L CL MMOL/L Group: 5 C92751 C92754 C92755 C92778 C92790 C92795 C92796 C92817 C92820 MEAN S.D. N Dose Level: 20.0 101 54 241 292 257 140 64 40 81 40 110 48 104 48 89 44 1 1 1 43 129 70. 0 9 83 84.4 9 Dosage Unit: ppm T-6295 0 10.3 1 11.0 0 10.9 0 10.2 4 11.2 0 10.4 0 10.1 0 10.1 0 10.4 6.1 7.2 7.3 5.8 7.2 7.4 7.5 7.1 7.3 1 10.5 1.3 .41 99 7.0 .61 9 147 155 149 144 151 146 145 146 150 148 3. 5 9 6.3 7.2 6.6 5.0 7.3 5.9 4.9 5.8 7.0 6.2 .89 9 112 112 109 108 112 107 108 108 111 110 2.1 9 Covance 6329-183 3M T-6295 1634 Appendix 5 Individual Clinical Chemistry Data Females Week 53 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER GLU MG/DL UN MG/DL CREAT MG/DL T PRO G/DL ALB G/DL GLOB G/DL T BILI MG/DL CHOL MG/DL Group: 1 C92862 C92876 C92889 C92894 C92899 C92910 C92917 C92922 C92923 C92930 MEAN S.D. N Group: 2 C92931 C92938 C92946 C92952 C92954 C92958 C92964 C92972 C92985 C92990 MEAN S.D. N Dose Level: 0 119 99 117 96 99 118 112 127 111 113 111 10.1 10 10 13 9 11 13 15 10 7 11 8 11 2.5 10 Dose Level: 0.5 112 12 97 18 115 13 91 15 98 13 95 12 113 14 106 12 98 11 100 10 102 8.4 10 13 2.3 10 Dosage Unit: ppm T-6295 .7 9.1 6.4 .7 7.1 5.1 .8 6 . 1 4.3 .7 7.4 5.5 .9 7.3 5.3 .7 8.8 6.5 .6 9.3 6.5 .6 8.0 6. 1 .6 6.9 4.8 .6 7.4 4.7 .7 7.7 5.5 .10 1.04 .81 10 10 10 Dosage Unit: ppm T-6295 .4 7.5 5.6 .8 8.6 6. 1 1.3 8. 6 6.4 .8 8.5 6.0 .5 8.0 5.7 .7 8.5 6. 2 .7 7.9 5.5 .7 7.4 5.3 .8 8.3 5.9 .5 8. 1 5.7 .7 8. 1 5.8 .25 .44 .34 10 10 10 2.7 2.0 1.8 1.9 2.0 2.3 2.8 1.9 2.1 2.7 2.2 .38 10 1.9 2.5 2.2 2.5 2.3 2.3 2.4 2.1 2.4 2.4 2.3 .19 10 . 1 144 .0 95 .0 84 .0 109 . 1 108 .2 126 . 1 137 .0 102 .0 122 .0 136 .0 116 .07 19 10 10 .4 72 .0 137 .1 88 .3 150 .3 97 . 1 92 .2 106 . 1 91 .0 106 .0 142 .2 108 .14 26 10 10 Covance 6329-183 3M T-6295 1635 Appendix 5 Individual Clinical Chemistry Data Females Week 53 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER GLU MG/DL UN MG/DL CREAT MG/DL T PRO G/DL ALB G/DL GLOB G/DL T BILI MG/DL CHOL MG/DL Group: 3 C92992 C92997 C93012 C93022 C93027 C93029 C93032 C93042 C93046 C93048 MEAN S.D. N Group: 4 C93052 C93054 C93060 C93078 C93081 C93083 C93091 C93095 C93097 C93110 MEAN S.D. N Dose Level: 2. 0 100 12 98 16 70 14 103 9 92 14 85 16 117 12 100 12 107 14 90 15 96 12.9 10 13 2.2 10 Dose Level: 5. 0 91 18 98 15 106 15 96 11 94 16 98 13 96 13 85 10 105 17 102 10 97 6.3 10 14 2.9 10 Dosage Unit: ppm T-6295 .8 1.0 .5 .9 .8 .9 .9 .7 1.0 .8 .8 .15 10 8.1 8. 6 8.2 8.2 8. 0 7.6 7.7 7.4 8. 8 7.6 8. 0 .45 10 5.3 5.3 5.1 6.2 5.7 5.1 5.2 5.3 6.3 5.7 5.5 .44 10 Dosage Unit: ppm T-6295 .8 8. 1 5.8 .6 8.4 5.9 .5 8.7 6. 2 .8 8. 2 5.9 .8 7.6 5.3 .8 8.3 6.4 .9 8. 2 5.6 .4 7.8 5.9 .9 8. 2 5.8 1. 0 8.4 5.5 .8 8. 2 5.8 .19 .31 .32 10 10 10 2.8 3.3 3.1 2.0 2.3 2.5 2.5 2.1 2.5 1.9 2.5 .46 10 2.3 2.5 2.5 2.3 2.3 1.9 2.6 1.9 2.4 2.9 2.4 .30 10 .1 121 .0 130 .2 100 .0 89 . 1 91 .3 96 .0 77 .2 118 .0 106 .0 85 .1 101 . 1 1 17 10 10 .0 81 .0 90 .2 155 . 1 115 .0 58 .3 86 .2 96 .0 80 .2 104 .3 230 .1 110 .13 49 10 10 Covance 6329-183 3M T-6295 1636 Appendix 5 Individual Clinical Chemistry Data Females Week 53 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER GLU MG/DL UN MG/DL CREAT MG/DL T PRO G/DL ALB G/DL GLOB G/DL T BILI MG/DL CHOL MG/DL Group: 5 C93112 C93116 C93117 C93120 C93130 C93138 C93147 C93154 C93163 C93171 MEAN S.D. N Dose Level : 20.0 85 15 100 14 107 22 84 15 104 19 103 13 92 17 86 17 109 13 105 17 98 9.8 10 16 2.8 10 Dosage Unit: ppm T-6295 .7 .6 .7 .6 .8 .9 .9 1.0 .6 .7 .8 .14 10 7.5 8.4 8.9 6.6 7.7 8.5 8.7 6.9 7.9 7.6 7.9 .76 10 5.3 6.8 5.9 4.4 5.8 6.2 6.5 4.3 5.8 5.7 5.7 .81 10 2.2 1.6 3.0 2.2 1.9 2.3 2.2 2.6 2.1 1.9 2.2 .39 10 .0 89 .1 86 .0 141 .0 67 .0 63 .3 48 . 1 87 .0 59 .0 73 .0 81 .0 79 .10 25 10 10 Covance 6329-183 3M T-6295 1637 Appendix 5 Individual Clinical Chemistry Data Females Week 53 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER AST/SGOT ALT/SGPT IU/L IU/L GGT IU/L CA MG/DL I PHOS MG/DL NA MMOL/L K MMOL/L CL MMOL/L Group: 1 C92862 C92876 C92889 C92894 C92899 C92910 C92917 C92922 C92923 C92930 MEAN S.D. N Group: 2 C92931 C92938 C92946 C92952 C92954 C92958 C92964 C92972 C92985 C92990 MEAN S.D. N 0Dose Level: 129 54 135 42 82 42 11838 39 29 283 133 76 32 103 44 105 52 79 34 119 61.0 10 50 30. 2 10 Dose Level: 0.5 102 45 137 42 10918 101 38 38 52 123 42 126 39 104 32 128 52 132 53 115 1105.3 43 107. 1 Dosage Unit: ppm T-6295 0 10.8 000 111000...004 100000 10.5 11.4 11.4 10.4 9.9 10.7 7.5 66..04 4.7 4.6 66..14 5.0 56..24 0 10.6 .3 .54 10 10 5.8 .90 10 Dosage Unit: ppm T-6295 00 1101..82 1 12.2 00 1111..04 0 11.1 1110 10.9 111011...203 6.1 6.7 7.6 5.7 4.9 5.7 4.8 5.6 5.6 5.5 0 11.1 10 .5 1.048 5.8 1.083 147 142 145 146 144 150 147 144 143 147 146 2.4 10 5.1 5.2 5.6 4.6 46..06 5.4 5.4 4.8 4.7 5.1 .47 10 144 149 156 145 144 148 145 148 145 146 147 103.6 66..03 8.5 5.3 5.5 5.1 56..01 5.8 4.9 5.8 1.1004 102 111007 107 106 109 103 108 104 107 1062 10 104 105 111031 107 103 110141 107 105 106 103 Covance 6329-183 3M T-6295 1638 Appendix 5 Individual Clinical Chemistry Data Females Week 53 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER AST/SGOT ALT/SGPT IU/L IU/L GGT IU/L CA MG/DL I PHOS MG/DL NA MMOL/L K MMOL/L CL MMOL/L Group: 3 C92992 C92997 C93012 C93022 C93027 C93029 C93032 C93042 C93046 C93048 MEAN S.D. N Group: 4 C93052 C93054 C93060 C93078 C93081 C93083 C93091 C93095 C93097 C93110 MEAN S.D. N Dose Level: 2.0 85 109 148 119 137 158 107 124 115 111 121 21.4 10 35 40 53 66 80 64 39 65 46 52 54 14.5 10 Dose Level: 5.0 130 26 121 75 153 84 128 55 101 40 78 30 107 35 77 49 84 34 98 36 108 25.1 10 46 19.5 10 Dosage Unit: ppm T-6295 0 0 0 0 0 0 3 3 0 0 1 1.3 10 10.4 11.0 10.7 10.9 11.1 10.6 10.8 10.9 10.9 10.4 10.8 .24 10 5.1 6.3 5.9 5.0 6.7 5.3 5.2 5.8 5.1 5.9 5.6 .58 10 Dosage Unit: ppm T-6295 0 10.8 0 10.9 0 11.6 4 10.8 4 10.9 0 11.1 2 11.1 0 10.5 0 11.1 0 11.2 5.3 5.6 6.7 4.1 6.3 4.8 5.9 5.3 5.8 5.4 1 1.7 10 11.0 .29 10 5.5 .74 10 145 151 145 149 148 147 148 145 145 149 147 2.1 10 4.6 6.0 4.8 5.2 5.4 5.3 4.9 4.6 4.6 5.3 5.1 .45 10 146 147 149 145 147 145 150 146 148 145 147 1.8 10 5.1 4.5 6.1 5.2 6.6 5.3 6.1 5.1 5.5 4.8 5.4 .65 10 106 109 107 108 108 108 103 104 103 110 107 2 10 111 106 107 105 108 109 111 111 109 101 108 3 10 Covance 6329-183 3M T-6295 1639 Appendix 5 Individual Clinical Chemistry Data Females Week 53 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER AST/SGOT ALT/SGPT IU/L IU/L GGT IU/L CA MG/DL I PHOS MG/DL NA MMOL/L K MMOL/L CL MMOL/L Group: 5 C93112 C93116 C93117 C93120 C93130 C93138 C93147 C93154 C93163 C93171 MEAN S.D. N Dose Level: 20.0 99 42 73 36 88 86 129 63 77 27 79 37 98 49 112 49 80 34 95 36 93 17. 5 10 46 17.3 10 Dosage Unit: ppm T-6295 0 10.5 0 11.1 0 10.9 0 10.0 0 10.9 0 10.5 0 11.2 0 9.8 1 10.5 0 10.2 5.2 5.5 5.9 6.0 6.8 6.1 7.1 5.8 5.9 5.0 0 .3 10 10.6 .47 10 5.9 .65 10 145 148 151 145 148 144 153 146 145 144 147 3. 1 10 4.6 4.8 5.8 4.8 6.7 5.8 7.2 4.8 4.3 4.5 5.3 1.00 10 108 105 109 112 112 106 112 113 109 110 110 2 10 Covance 6329-183 3M T-6295 1640 Appendix 5 Individual Clinical Chemistry Data Females Week 103 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER CHOL MG/DL TRIG MG/DL Group: 3 C92991 C93003 C93007 C93011 C93022 C93039 C93040 C93047 C93048 MEAN S.D. N Dose Level : 2.0 139 101 66 247 107 73 145 285 145 211 133 174 202 108 79 180 100 143 124 41.1 9 169 70 9 Dosage Unit: ppm T-6295 Covance 6329-183 3M T-6295 1641 Appendix 5 Individual Clinical Chemistry Data Males Week 105 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER CHOL MG/DL TRIG MG/DL Group: 1 C92503 C92505 C92510 C92513 C92514 C92518 C92541 C92542 C92544 C92551 C92569 MEAN S.D. N Group: 2 C92577 C92583 C92590 C92597 C92598 C92601 C92602 C92619 C92623 C92626 MEAN S.D. N Dose Level: 0 101 42 60 124 116 220 136 187 123 216 328 207 110 54 187 262 120 62 184 590 220 230 153 73. 5 11 199 151 11 Dose Level: 0.5 189 134 239 322 147 195 84 108 241 96 125 255 116 193 137 306 86 153 76 110 144 60. 8 10 187 82 10 Dosage Unit: ppm T-6295 Dosage Unit: ppm T-6295 Covance 6329-183 3M T-6295 1642 Appendix 5 Individual Clinical Chemistry Data Males Week 105 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER CHOL MG/DL TRIG MG/DL Group: 3 C92631 C92633 C92637 C92641 C92642 C92643 C92644 C92649 C92653 C92657 C92659 C92660 C92669 C92674 C92682 C92683 C92690 MEAN S.D. N Dose Level: 2.0 121 218 147 86 202 360 134 182 123 71 181 423 153 375 89 86 198 186 117 152 75 58 128 132 139 114 81 188 91 383 94 100 123 272 129 38.4 17 199 120 17 Dosage Unit: ppm T-6295 Covance 6329-183 3M T-6295 1643 Appendix 5 Individual Clinical Chemistry Data Males Week 105 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER CHOL MG/DL TRIG MG/DL Group: 4 C92691 C92693 C92694 C92695 C92696 C92698 C92699 C92700 C92702 C92704 C92705 C92708 C92716 C92717 C92724 C92726 C92730 C92735 C92736 C92739 C92740 C92742 C92747 C92748 C92750 MEAN S.D. N Dose Level : 5.0 125 183 86 79 97 64 114 184 102 96 103 81 87 145 98 115 135 144 129 140 153 184 144 264 101 151 268 277 154 237 127 137 154 75 119 215 157 231 67 137 77 86 163 218 49 32 145 117 126 169 123 42.7 25 150 65.3 25 Dosage Unit: ppm T-6295 Covance 6329-183 3M T-6295 1644 Appendix 5 Individual Clinical Chemistry Data Males Week 105 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER CHOL MG/DL TRIG MG/DL Group: 5 C92753 C92757 C92761 C92762 C92774 C92775 C92776 C92780 C92782 C92786 C92788 C92791 C92792 C92800 C92802 C92803 C92804 C92808 C92809 C92814 C92815 C92818 MEAN S.D. N Dose Level : 20.0 97 116 102 107 163 428 99 87 126 204 96 203 43 77 139 88 134 237 185 212 80 38 201 421 91 146 98 142 33 53 67 35 90 1 1 1 150 213 214 410 62 55 213 267 102 76 118 52.2 22 169 121.9 22 Dosage Unit: ppm T-6295 Covance 6329-183 3M T-6295 1645 Appendix 5 Individual Clinical Chemistry Data Males Week 105 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER CHOL MG/DL TRIG MG/DL Group: 6 C92821 C92823 C92827 C92829 C92833 C92834 C92848 C92851 C92853 C92856 MEAN S.D. N Dose Level : 20.0 94 88 175 145 173 163 104 69 125 147 200 206 85 42 168 169 155 141 174 196 145 40.1 10 137 53 10 Dosage Unit: ppm T-6295 Covance 6329-183 3M T-6295 1646 Appendix 5 Individual Clinical Chemistry Data Females Week 105 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER CHOL MG/DL TRIG MG/DL Group: 1 C92866 C92867 C92868 C92869 C92871 C92873 C92874 C92877 C92881 C92882 C92885 C92890 C92892 C92895 C92896 C92897 C92906 C92907 C92909 C92911 C92912 C92913 C92925 C92927 MEAN S.D. N Dose Level: 0 116 52 151 149 115 101 114 98 162 203 108 102 113 91 100 138 114 181 142 123 218 234 94 80 108 108 97 72 123 101 182 46 102 51 147 405 90 188 120 118 83 44 105 55 222 98 58 118 124 39.6 24 123 78 24 Dosage Unit: ppm T-6295 Covance 6329-183 3M T-6295 1647 Appendix 5 Individual Clinical Chemistry Data Females Week 105 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER CHOL MG/DL TRIG MG/DL Group: 2 C92936 C92938 C92946 C92953 C92957 C92960 C92964 C92970 C92973 C92974 C92975 C92983 C92984 C92986 C92988 MEAN S.D. N Dose Level : 0.5 113 51 117 89 138 399 128 100 81 29 116 116 144 130 104 34 119 67 117 61 140 282 89 30 157 110 180 104 110 111 124 25.4 15 114 100 . 1 15 Dosage Unit: ppm T-6295 Covance 6329-183 3M T-6295 1648 Appendix 5 Individual Clinical Chemistry Data Females Week 105 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER CHOL MG/DL TRIG MG/DL Group: 4 C93059 C93060 C93062 C93063 C93072 C93077 C93078 C93079 C93080 C93081 C93090 C93098 C93101 C93102 C93107 MEAN S.D. N Dose Level : 5.0 100 160 142 100 126 45 72 73 128 86 115 91 175 63 101 408 88 203 91 97 83 82 169 158 106 207 100 191 118 71 114 29.8 15 136 92 .5 15 Dosage Unit: ppm T-6295 Covance 6329-183 3M T-6295 1649 Appendix 5 Individual Clinical Chemistry Data Females Week 105 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER CHOL MG/DL TRIG MG/DL Group: 5 C93113 C93115 C93124 C93125 C93133 C93135 C93137 C93139 C93140 C93146 C93149 C93151 C93153 C93157 C93158 C93161 C93162 C93164 C93165 C93166 C93168 C93174 C93177 C93178 C93179 MEAN S.D. N Dose Level: 20.0 122 188 160 124 113 77 114 32 61 80 94 56 38 54 76 104 340 585 117 60 149 55 86 51 127 132 119 160 70 59 89 58 80 79 71 56 59 29 86 54 103 117 68 49 84 48 71 38 77 47 103 57.2 25 96 109 25 Dosage Unit: ppm T-6295 Covance 6329-183 3M T-6295 1650 Appendix 5 Individual Clinical Chemistry Data Females Week 105 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER CHOL MG/DL TRIG MG/DL Group: 6 C93181 C93182 C93183 C93184 C93185 C93190 C93192 C93197 C93198 C93201 C93202 C93205 C93209 C93211 C93216 C93219 C93220 MEAN S.D. N Dose Level : 20.0 111202 133 172 78 55 81 124 129 128 139 56 141 175 118 98 94 306 79 89 77 146 14856 148 134 110111 63 148 124 310 118 241 109 23.3 17 149 76.2 17 Dosage Unit: ppm T-6295 Covance 6329-183 3M T-6295 1651 Appendix 5 Individual Clinical Urinalysis Data Males Week 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-62 ANIMAL NUMBER U VOL ML SP GR U PH Group: 1 C92505 C92506 C92524 C92526 C92529 C92542 C92543 C92546 C92548 C92553 MEAN S.D. N Group: 2 C92573 C92574 C92575 C92585 C92586 C92587 C92610 C92613 C92615 C92618 MEAN S.D. N Dose Level: 0 11.5 34.2 48.8 a 22.6 17.5 19.2 17.4 16.5 9.5 16.8 1.013 1.007 1.005 1.007 1.010 1.010 1.010 1.015 1.016 1.012 21.4 11.72 10 1.010 .0036 10 Dose Level: 0.5 13.2 14.4 12.2 17.5 48.5 a 18.3 42.6 6.4 14.5 38.5 22.6 14.76 10 1.017 1.017 1.022 1.013 1.002 1.012 1.004 1.027 1.016 1.005 1.014 .0080 10 Dossaagg<e Unit: ppm T-6295 7.0 7.0 7.0 7.0 7.0 7.0 7.5 7.0 7.0 7.0 7.0 .16 10 Dossaagg<e Unit: ppm T-6295 7.0 7.0 7.0 7.0 7.0 7.5 6.5 7.0 7.0 7.0 7.0 .24 10 a Sample was overflowing at the time of collection. 1652 Covance 6329-183 3M T-6295 Appendix 5 Individual Clinical Urinalysis Data Males Week 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295 ANIMAL NUMBER U VOL ML SP GR U PH Group: 3 C92632 C92646 C92650 C92652 C92657 C92668 C92676 C92678 C92684 C92686 MEAN S.D. N Group: 4 C92709 C92715 C92716 C92718 C92725 C92731 C92732 C92734 C92744 C92746 MEAN S.D. N Dose Level: 2.0 10.3 48.1 a 48.4 a 20.2 48.3 a 23.3 16.1 9.3 14.1 15.2 25.3 16.35 10 1.021 1.001 1.000 1.010 1.003 1.009 1.013 1.020 1.013 1.013 1.010 .0073 10 Dose Level: 5.0 15.5 27.3 49.9 a 49.5 a 24.5 48.0 a 23.5 11.2 42.8 25.5 1.015 1.010 1.004 1.002 1.008 1.004 1.008 1.020 1.006 1.010 31.8 14.52 10 1.009 .0055 10 Dosage 6.5 7.0 7.0 7.0 7.0 7.0 6.5 7.0 7.5 7.5 7.0 .33 10 Dosage Unit: ppm T-6295 7.0 7.5 7.0 7.0 7.0 7.0 7.5 7.0 7.0 7.5 7.2 .24 10 a Sample was overflowing at the time of collection. 1653 Covance 6329-183 3M T-6295 Appendix 5 Individual Clinical Urinalysis Data Males Week 4 DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS;: T-6295) IN RATS ANIMAL NUMBER U VOL ML SP GR U PH Group: 5 C92752 C92759 C92779 C92784 C92793 C92798 C92799 C92805 C92807 C92811 MEAN S.D. N Dose Level: 20.0 29.0 24.0 6.0 26.7 17.0 28.6 39.7 19.9 10.2 24.7 22.6 9.78 10 1.006 1.009 1.029 1.010 1.016 1.009 1.006 1.013 1.017 1.009 1.012 .0069 10 Dosage Unit: ppm T-6295 7.0 7.0 7.0 7.0 7.0 7.0 7.0 7.0 7.0 7.0 7.0 .00 10 Covance 6329-183 3M T-6295 1654 Appendix 5 Individual Clinical Urinalysis Data Males Week 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER U PRO MG/DL U GLU U KET U BILI U BLOOD UROBILI Group: 1 C92505 C92506 C92524 C92526 C92529 C92542 C92543 C92546 C92548 C92553 Group: 2 C92573 C92574 C92575 C92585 C92586 C92587 C92610 C92613 C92615 C92618 Dose Level: 0 10 10 010 10 10 10 10 10 30 - Dose Level: 0.5 10 30 10 10 010 030 10 10 - Dosage Unit: ppm T-6295 +-+ --------------+-+-- Dosage Unit: ppm T-6295 +-+-+---------+-+---- - - Covance 6329-183 3M T-6295 1655 Appendix 5 Individual Clinical Urinalysis Data Males Week 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER U PRO MG/DL U GLU U KET U BILI U BLOOD UROBILI Group: 3 C92632 C92646 C92650 C92652 C92657 C92668 C92676 C92678 C92684 C92686 Group: 4 C92709 C92715 C92716 C92718 C92725 C92731 C92732 C92734 C92744 C92746 Dose Level: 2.0 10 0010 10 10 10 10 10 10 - Dose Level: 5.0 10 10 10 010 010 10 10 30 - Dosage Unit: ppm T-6295 +---------------+-+ --- Dosage Unit: ppm T-6295 +-------+-------+---- - - Covance 6329-183 3M T-6295 1656 Appendix 5 Individual Clinical Urinalysis Data Males Week 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER U PRO MG/DL U GLU U KET U BILI U BLOOD UROBILI Group: 5 C92752 C92759 C92779 C92784 C92793 C92798 C92799 C92805 C92807 C92811 Dose Level: 20.0 10 10 30 10 10 10 10 10 10 10 - Dosage Unit: ppm T-6295 + +-+---------+-+-+-- - Covance 6329-183 3M T-6295 1657 Appendix 5 Individual Clinical Urinalysis Data Males Week 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER RBC WBC EPITH BACT CASTS CRYSTALS PER HPF PER HPF PER HPF PER HPF PER LPF PER LPF1 URINE COMMENTS APP1 Group: 1 Dose Level: 0 Dosage Unit: ppm T-6295 C92505 0 0 1 1 0 3D BJ Q C92506 0 0 1 2 0 0 AJ PQ C92524 0 0 0 1 0 0 AJ Q C92526 0 0 1 2 0 0 BK Q C92529 0 0 1 1 0 4D BJ Q C92542 0 0 1 1 0 4D BK PQ C92543 0 0 1 1 0 4D BJ Q C92546 0 0 0 2 0 4D BK PQ C92548 0 0 0 2 0 3D CJ Q C92553 0 0 0 1 0 4D BJ Q Group: 2 Dose Level: 0.5 Dosage Unit: ppm T-6295 C92573 0 0 0 3 0 4D CK Q C92574 0 0 1 1 0 3D CK Q C92575 0 0 1 2 0 4D CJ P C92585 0 0 1 1 0 2D BK PQ C92586 0 0 0 2 0 0 AJ Q C92587 0 0 0 1 0 0 CJ - C92610 0 0 1 1 0 0 BJ Q C92613 0 0 1 3 0 4D CK PQ C92615 0 0 0 2 0 4D CJ PQ C92618 0 0 1 1 0 0 AJ - Covance 6329-183 3M T-6295 1658 Appendix 5 Individual Clinical Urinalysis Data Males Week 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER RBC WBC EPITH BACT CASTS CRYSTALS PER HPF PER HPF PER HPF PER HPF PER LPF PER LPF1 URINE COMMENTS APP1 Group: 3 C92632 C92646 C92650 C92652 C92657 C92668 C92676 C92678 C92684 C92686 Group: 4 C92709 C92715 C92716 C92718 C92725 C92731 C92732 C92734 C92744 C92746 Dose Level: 2.0 00 00 00 00 00 00 00 00 00 00 Dose Level: 5.0 00 00 00 00 00 00 00 00 00 00 Dosage Unit: ppm T-6295 120 02 0 01 0 01 0 02 0 02 0 110 03 0 110 01 0 Dosage Unit: ppm T-6295 02 0 01 0 110 110 110 03 0 01 0 02 0 03 0 110 0 0 0 0 1B 2D 0 4D 4D 4D 2B4D 4D 0 0 1D 0 0 4D 4D 2D CK AJ AJ BJ AJ BJ BJ CK CK CJ CK BK AJ AJ BJ AJ BJ CK BK BJ Q Q Q PQ Q PQ Q PQ PQ - Q PQ Q Q Q Q Q PQ PQ Q Covance 6329-183 3M T-6295 1659 Appendix 5 Individual Clinical Urinalysis Data Males Week 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER RBC WBC EPITH BACT CASTS CRYSTALS PER HPF PER HPF PER HPF PER HPF PER LPF PER LPF1 URINE COMMENTS APP1 Group: 5 C92752 C92759 C92779 C92784 C92793 C92798 C92799 C92805 C92807 C92811 Dose Level: 20.0 00 00 00 00 00 00 00 00 00 00 Dosage Unit: ppm T-6295 120 01 0 110 01 0 02 0 03 0 03 0 01 0 120 01 0 0 1D 3D 1D 4D 0 0 0 4D 0 AJ PQ BJ Q CK Q BJ PQ CK PQ BJ Q BJO Q BJ PQ CJ PQ BJ Q Covance 6329-183 3M T-6295 1660 Appendix 5 Individual Clinical Urinalysis Data Females Week 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER U VOL ML SP GR U PH Group: 1 C92861 C92864 C92876 C92877 C92882 C92883 C92914 C92919 C92925 C92926 MEAN S.D. N Group: 2 C92941 C92945 C92950 C92953 C92959 C92964 C92968 C92969 C92976 C92982 MEAN S.D. N Dose Level: 0 9.0 3.4 2.2 6.6 6.4 22.5 2.7 45.2 a 9.5 1.9 1.016 1.023 1.036 1.018 1.018 1.008 1.026 1.001 1.015 1.030 10.9 13.48 10 1.019 .0103 10 Dose Level: 0.5 1.5 47.5 a 15.5 6.7 10.0 7.2 9.6 3.5 42.3 a 8.5 1.036 1.000 1.007 1.020 1.008 1.019 1.016 1.022 1.003 1.017 15.2 16.13 10 1.015 .0106 10 Dosage Unit: ppm T-6295 7.0 7.0 6.5 6.5 6.5 7.0 7.0 7.0 6.5 6.5 6.8 .26 10 Dosaaggee Unit: ppm T-6295 6.5 7.0 7.0 7.0 6.5 7.0 7.0 7.0 7.0 6.5 6.8 .24 10 a Sample was overflowing at the time of collection. 1661 Covance 6329-183 3M T-6295 Appendix 5 Individual Clinical Urinalysis Data Females Week 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER U VOL ML SP GR U PH Group: 3 C92993 C92996 C92998 C93004 C93006 C93008 C93023 C93031 C93033 C93045 MEAN S.D. N Group: 4 C93058 C93067 C93070 C93074 C93075 C93083 C93084 C93089 C93094 C93105 MEAN S.D. N Dose Level: 2.0 4.0 46.0 a 5.0 6.2 5.9 7.7 49.3 a 4.1 6.4 7.5 1.022 1.001 1.012 1.021 1.014 1.016 1.000 1.023 1.021 1.023 14.2 17.68 10 1.015 .0087 10 Dose Level: 5.0 6.5 14.1 5.2 8.0 9.0 18.2 12.5 11.2 29.0 18.0 1.017 1.010 1.021 1.023 1.016 1.008 1.008 1.018 1.006 1.011 13.2 7.12 10 1.014 .0060 10 Dosage Unit: ppm T-6295 6.5 6.5 7.0 6.5 7.0 7.0 7.0 7.0 7.0 6.5 6.8 .26 10 Dosage Unit: ppm T-6295 7.0 7.0 6.5 7.0 7.0 6.5 7.0 7.0 7.0 6.5 6.8 .24 10 a Sample was overflowing at the time of collection. 1662 Covance 6329-183 3M T-6295 Appendix 5 Individual Clinical Urinalysis Data Females Week 4 DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS;: T-6295) IN RATS ANIMAL NUMBER U VOL ML SP GR U PH Group: 5 C93114 C93123 C93142 C93144 C93151 C93155 C93158 C93159 C93164 C93175 MEAN S.D. N Dose Level: 20.0 28.7 9.5 9.8 10.0 5.1 4.1 43.0 a 48.4 a 10.2 6.5 1.005 1.014 1.015 1.012 1.019 1.026 1.002 1.001 1.014 1.022 17.5 16.39 10 1.013 .0083 10 Dosage Unit: ppm T-6295 7.0 7.0 7.0 6.5 6.5 6.5 7.0 7.0 7.0 6.5 6.8 .26 10 a Sample was overflowing at the time of collection. Covance 6329-183 3M T-6295 1663 Appendix 5 Individual Clinical Urinalysis Data Females Week 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER U PRO MG/DL U GLU U KET U BILI U BLOOD UROBILI Group: 1 C92861 C92864 C92876 C92877 C92882 C92883 C92914 C92919 C92925 C92926 Group: 2 C92941 C92945 C92950 C92953 C92959 C92964 C92968 C92969 C92976 C92982 Dose Level: 0 0 11000 - 10 - 0- 10 - 0- 0- 10 - Dose Level: 0.5 3000 - 11000 - 10 - 10 - 0- 0- Dosage Unit: ppm T-6295 ------------------- Dosage Unit: ppm T-6295 - ---------------------------- Covance 6329-183 3M T-6295 1664 Appendix 5 Individual Clinical Urinalysis Data Females Week 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER U PRO MG/DL U GLU U KET U BILI U BLOOD UROBILI Group: 3 C92993 C92996 C92998 C93004 C93006 C93008 C93023 C93031 C93033 C93045 Group: 4 C93058 C93067 C93070 C93074 C93075 C93083 C93084 C93089 C93094 C93105 Dose Level: 2.0 10 00010 0010 10 0- Dose Level: 5.0 10 010 10 000000- Dosage Unit: ppm T-6295 ------------------- Dosage Unit: ppm T-6295 - ---------------------------- Covance 6329-183 3M T-6295 1665 Appendix 5 Individual Clinical Urinalysis Data Females Week 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER U PRO MG/DL U GLU U KET U BILI U BLOOD UROBILI Group: 5 C93114 C93123 C93142 C93144 C93151 C93155 C93158 C93159 C93164 C93175 Dose Level: 20.0 0 00010 00000- Dosage Unit: ppm T-6295 ------------------- - Covance 6329-183 3M T-6295 1666 Appendix 5 Individual Clinical Urinalysis Data Females Week 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER RBC WBC EPITH BACT CASTS CRYSTALS PER HPF PER. HPF PER HPF PER HPF PER LPF PER LPF1 URINE COMMENTS APP1 Group: 1 C92861 C92864 C92876 C92877 C92882 C92883 C92914 C92919 C92925 C92926 Group: 2 C92941 C92945 C92950 C92953 C92959 C92964 C92968 C92969 C92976 C92982 Dose Level: 0 00 00 00 01 00 00 00 00 00 01 Dose Level: 0.5 00 00 01 00 01 01 00 01 01 00 Dosage Unit: ppm T-6295 01 0 110 110 110 110 120 120 01 0 110 01 0 Dosage Unit: ppm T-6295 110 01 0 120 110 01 0 01 0 120 01 0 110 01 0 2D 2D 2D 1B1D 1D 0 4D 0 2B 0 0 0 0 3D 0 2D 4D 3D 0 2D CJ CJ CK CJ CJ AJ CJ AJ CJ CJ CJ AJ BJ CK BJ CJ CJ CJ AJ CK P P P P P P P P - Covance 6329-183 3M T-6295 1667 Appendix 5 Individual Clinical Urinalysis Data Females Week 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER RBC WBC EPITH BACT CASTS CRYSTALS PER HPF PER HPF PER HPF PER HPF PER LPF PER LPF1 URINE COMMENTS APP1 Group: 3 Dose Level: 2.0 Dosage Unit: ppm T-6295 C92993 0 1 1 1 C92996 0 0 1 1 C92998 0 1 1 1 C93004 0 0 1 1 C93006 0 0 0 1 C93008 0 0 0 1 C93023 0 0 0 1 C93031 0 0 0 1 C93033 0 0 1 1 C93045 0 0 1 1 0 1D CJ P 0 0 AJ 0 0 BJ P 0 1D CJ 0 3D BJ 0 2D CJ 0 0 AJ 0 4D CJ P 0 4D CK 0 4D CK P Group: 4 Dose Level: 5.0 Dosage Unit: ppm T-6295 C93058 0 0 0 1 0 4D CK - C93067 0 0 0 1 0 3D BJ - C93070 0 1 1 2 0 0 CK P C93074 0 1 1 1 0 4D CK P C93075 0 1 1 1 0 4D CJ P C93083 0 1 01 0 1B BJ P C93084 0 1 1 1 0 0 BJ P C93089 0 1 01 1G 2D CJ P C93094 0 1 1 2 0 0 AJ - C93105 0 1 1 2 0 1D BJ P Covance 6329-183 3M T-6295 1668 Appendix 5 Individual Clinical Urinalysis Data Females Week 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER RBC WBC EPITH BACT CASTS CRYSTALS PER HPF PER HPF PER HPF PER HPF PER LPF PER LPF1 URINE COMMENTS APP1 Group: 5 Dose Level: 20.0 Dosage: Unit: ppm T-6295 C93114 0 0 0 1 0 0 BJ - C93123 0 0 1 1 0 4D BJ - C93142 0 0 1 1 0 4D BJ - C93144 0 0 1 2 0 0 BJ P C93151 0 0 1 1 0 2D CJ P C93155 0 0 1 1 0 2D CJ P C93158 0 0 0 1 0 1B AJ - C93159 0 0 0 1 0 0 AJ - C93164 0 1 1 1 0 1D BJ - C93175 0 1 1 1 0 2D CJ - Covance 6329-183 3M T-6295 1669 Appendix 5 Individual Clinical Urinalysis Data Males Week 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER U VOL ML SP GR U PH Group: 1 C92508 C92509 C92511 C92521 C92527 C92528 C92532 C92535 C92537 C92562 MEAN S.D. N Group: 2 C92583 C92593 C92598 C92600 C92612 C92616 C92621 C92624 C92627 C92629 MEAN S.D. N Dose Level: 0 30.2 19.1 31.4 9.1 26.0 49.5a 20.0 4.5 49.5 a 49.1 a 1.010 1.012 1.009 1.027 1.015 1.003 1.015 1.035 1.006 1.008 28.8 16.45 10 1.014 .0099 10 Dose Level: 0.5 16.8 46.3 a 23.5 7.4 49.3 a 28.7 3.4 17.0 35.6 7.3 1.016 1.003 1.014 1.022 1.006 1.008 1.044 1.018 1.008 1.040 23.5 16.23 10 1.018 .0140 10 Dosa 7.5 7.5 7.0 7.0 7.5 7.0 7.0 6.5 7.0 7.5 7.2 .3 10 Dosa 7.5 6.5 7.0 7.0 7.5 7.5 6.5 7.5 7.0 7.0 7.1 .3 10 ppm T-6295 ppm T-6295 a Sample was overflowing at the time of collection. 1670 Covance 6329-183 3M T-6295 Appendix 5 Individual Clinical Urinalysis Data Males Week 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER U VOL ML SP GR U PH Group: 3 C92636 C92640 C92644 C92645 C92651 C92661 C92662 C92676 C92682 C92684 MEAN S.D. N Group: 4 C92711 C92713 C92714 C92716 C92719 C92722 C92726 C92728 C92741 C92748 MEAN S.D. N Dose Level: 2.0 46.5 a 6.8 9.4 9.7 14.8 40.5 10.1 2.0 20.5 47.5 a 20.8 17.37 10 1.004 1.025 1.026 1.022 1.021 1.007 1.020 1.032 1.016 1.003 1.018 .0099 10 Dose Level: 5.0 10.1 47.7 a 22.1 26.3 12.0 35.0 12.8 15.5 49.0 a 23.5 1.021 1.005 1.013 1.013 1.021 1.005 1.021 1.020 1.007 1.016 25.4 14.27 10 1.014 .0067 10 Dosage Unit: ppm T-6295 6.5 6.5 7.0 7.0 7.5 7.5 7.0 6.5 7.0 7.0 7.0 .37 10 Dosage Unit: ppm T-6295 7.0 7.0 7.0 7.5 6.5 6.5 7.0 7.0 7.5 7.0 7.0 .33 10 a Sample was overflowing at the time of collection. 1671 Covance 6329-183 3M T-6295 Appendix 5 Individual Clinical Urinalysis Data Males Week 14 DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS;: T-6295) IN RATS ANIMAL NUMBER U VOL ML SP GR U PH Group: 5 C92754 C92765 C92767 C92774 C92777 C92784 C92789 C92799 C92812 C92820 MEAN S.D. N Dose Level: 20.0 9.3 9.8 37.0 12.5 48.1 12.0 5.5 12.7 7.6 30.8 1.020 1.024 1.010 1.020 1.007 1.021 1.028 1.025 1.027 1.012 18.5 14.64 10 1.019 .0073 10 Dosage Unit: ppm T-6295 7.0 7.0 7.0 7.0 7.0 7.0 6.5 6.5 7.0 7.5 7.0 .28 10 Covance 6329-183 3M T-6295 1672 Appendix 5 Individual Clinical Urinalysis Data Males Week 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER U PRO MG/DL U GLU U KET U BILI U BLOOD UROBILI Group: 1 C92508 C92509 C92511 C92521 C92527 C92528 C92532 C92535 C92537 C92562 Group: 2 C92583 C92593 C92598 C92600 C92612 C92616 C92621 C92624 C92627 C92629 Dose Level: 0 10 10 10 30 30 010 30 010 - Dose Level: 0.5 30 010 30 010 30 30 10 30 - Dosage Unit: ppm T-6295 ------+ +-+-----+------ Dosage Unit: ppm T-6295 ------------+-+ +---+-- - - Covance 6329-183 3M T-6295 1673 Appendix 5 Individual Clinical Urinalysis Data Males Week 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER U PRO MG/DL U GLU U KET U BILI U BLOOD UROBILI Group: 3 C92636 C92640 C92644 C92645 C92651 C92661 C92662 C92676 C92682 C92684 Group: 4 C92711 C92713 C92714 C92716 C92719 C92722 C92726 C92728 C92741 C92748 Dose Level: 2.0 0 30 10 30 30 10 30 30 10 0- Dose Level: 5.0 30 10 10 10 10 10 30 30 10 10 - Dosage Unit: ppm T-6295 --+-+-----+-+------ Dosage Unit: ppm T-6295 +---+-+-----+-+------ - - Covance 6329-183 3M T-6295 1674 Appendix 5 Individual Clinical Urinalysis Data Males Week 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER U PRO MG/DL U GLU U KET U BILI U BLOOD UROBILI Group: 5 C92754 C92765 C92767 C92774 C92777 C92784 C92789 C92799 C92812 C92820 Dose Level: 20.0 10 30 10 10 10 30 10 30 30 10 - Dosage Unit: ppm T-6295 + ----+---+-+-+ --+ --+-- - Covance 6329-183 3M T-6295 1675 Appendix 5 Individual Clinical Urinalysis Data Males Week 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER RBC WBC EPITH BACT CASTS CRYSTALS PER HPF PER HPF PER HPF PER HPF PER LPF PER LPF1 URINE COMMENTS APP1 Group: 1 C92508 C92509 C92511 C92521 C92527 C92528 C92532 C92535 C92537 C92562 Group: 2 C92583 C92593 C92598 C92600 C92612 C92616 C92621 C92624 C92627 C92629 Dose Level: 0 00 00 01 00 00 00 00 00 00 00 Dose Level: 0.5 00 10 00 10 00 00 01 01 00 00 Dosage Unit: ppm T-6295 110 110 110 02 0 120 01 0 110 110 120 110 Dosage Unit: ppm T-6295 120 110 110 01 0 110 01 0 110 110 110 120 4D 4D 1B1D 4D 4D 0 4D 3D 2D 2D 4D 0 4D 4D 3D 3D 4D 4D 3D 3D BJ BJ BJ BK BK AJ BK CK AJ AJ BK AJ BK BK AJ BK DK BK BJ CK Q Q PQ P Q Q Q Q Q Q Q Q Q PQ Q Q PQ Q Q PQ Covance 6329-183 3M T-6295 1676 Appendix 5 Individual Clinical Urinalysis Data Males Week 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER RBC WBC EPITH BACT CASTS CRYSTALS PER HPF PER: HPF PER HPF PER HPF PER LPF PER LPF1 URINE COMMENTS APP1 Group: 3 Dose Level: 2.0 Dosage Unit:: ppm T-6295 C92636 C92640 C92644 C92645 C92651 C92661 C92662 C92676 C92682 C92684 0000000000 1000000000 1111111000 2222211111 0000000000 0 AJ Q 4D CK Q 4D CK Q 4D BK P 4D CK Q 4D AJ Q 03D BK PQ BJ Q 04D BK PQ AJ Q Group: 4 Dose Level: 5.0 Dosage Unit: ppm T-6295 C92711 C92713 C92714 C92716 C92719 C92722 C92726 C92728 C92741 C92748 0000000000 2100000000 1111111100 11111 31 2 1 1 0000000000 04D CK PQ AJ Q 1D BJ Q 4D BK Q 02D BK PQ AJ Q 3D BK Q 4D BK Q 3D AJ Q 4D BK Q Covance 6329-183 3M T-6295 1677 Appendix 5 Individual Clinical Urinalysis Data Males Week 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER RBC WBC EPITH BACT CASTS CRYSTALS PER HPF PER HPF PER HPF PER HPF PER LPF PER LPF1 URINE COMMENTS APP1 Group: 5 C92754 C92765 C92767 C92774 C92777 C92784 C92789 C92799 C92812 C92820 Dose Level: 20.0 00 00 00 00 00 00 21 00 00 00 Dosage Unit: ppm T-6295 110 01 0 120 01 0 110 120 110 110 120 120 4D 3D 0 4D 0 1B4D 1D 1D 4D 2B4D CK BK AJ CJ BJ CJ BK BJ BK BJ Q Q Q PQ Q PQ PQ Q PQ PQ Covance 6329-183 3M T-6295 1678 Appendix 5 Individual Clinical Urinalysis Data Females Week 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295 ANIMAL NUMBER U VOL ML SP GR U PH Group: 1 C92863 C92865 C92871 C92880 C92887 C92898 C92903 C92905 C92906 C92927 MEAN S.D. N Group: 2 C92935 C92940 C92944 C92948 C92956 C92962 C92967 C92978 C92986 C92987 MEAN S.D. N Dose Level: 0 4.3 7.0 .7 2.3 6.2 4.5 6.5 49.5a 48.5a 45.5 a 17.5 21.04 10 1.033 1.023 1.056 1.025 1.020 1.022 1.020 1.002 1.003 1.001 1.020 .0166 10 Dose Level: 0.5 3.2 18.5 49.9 a 7.0 12.3 49.2 a 6.9 11.5 6.5 3.1 1.030 1.006 1.003 1.020 1.015 1.003 1.021 1.019 1.018 1.022 16.8 17.86 10 1.016 .0090 10 Dosage i 7.0 7.0 7.0 6.5 6.5 6.5 7.0 6.5 7.0 6.5 6.8 .26 10 Dosage 7.0 8.0 7.0 6.5 7.5 6.5 6.5 6.5 7.0 7.0 7.0 .50 10 a Sample was overflowing at the time of collection. 1679 Covance 6329-183 3M T-6295 Appendix 5 Individual Clinical Urinalysis Data Females Week 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER U VOL ML SP GR U PH Group: 3 C92992 C92993 C93000 C93018 C93019 C93023 C93024 C93026 C93030 C93035 MEAN S.D. N Group: 4 C93051 C93064 C93071 C93076 C93078 C93085 C93089 C93090 C93093 C93109 MEAN S.D. N Dose Level: 2.0 18.8 3.6 5.0 8.0 20.4 4.2 5.0 3.4 2.7 7.1 7.8 6.43 10 1.008 1.024 1.019 1.015 1.010 1.026 1.019 1.029 1.033 1.027 1.021 .0082 10 Dose Level: 5.0 8.7 16.0 48.0 a 19.6 45.5 a 12.1 21.5 41.5 25.8 18.5 1.019 1.008 1.002 1.009 1.003 1.012 1.012 1.006 1.013 1.010 25.7 14.20 10 1.009 .0050 10 Dosage Unit: ppm T-6295 7.0 6.5 6.5 7.0 7.5 6.5 7.0 7.0 6.5 6.5 6.8 .35 10 Dosage Unit: ppm T-6295 6.5 7.0 6.5 7.0 7.0 6.5 7.0 7.5 6.5 6.5 6.8 .35 10 a Sample was overflowing at the time of collection. 1680 Covance 6329-183 3M T-6295 Appendix 5 Individual Clinical Urinalysis Data Females Week 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER U VOL ML SP GR U PH Group: 5 C93111 C93113 C93120 C93127 C93135 C93143 C93155 C93162 C93169 C93178 MEAN S.D. N Dose Level: 20.0 13.5 12.2 8.0 38.0 46.5a 6.0 1.5 13.2 7.2 49.0 a 1.014 1.010 1.018 1.003 1.004 1.022 1.043 1.012 1.020 1.005 19.5 17.83 10 1.015 .0119 10 Dosage 7.0 7.0 7.5 6.5 7.0 7.0 6.5 7.0 6.5 7.0 6.9 .32 10 ppm T-6295 a Sample was overflowing at the time of collection. Covance 6329-183 3M T-6295 1681 Appendix 5 Individual Clinical Urinalysis Data Females Week 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER U PRO MG/DL U GLU U KET U BILI U BLOOD UROBILI Group: 1 C92863 C92865 C92871 C92880 C92887 C92898 C92903 C92905 C92906 C92927 Group: 2 C92935 C92940 C92944 C92948 C92956 C92962 C92967 C92978 C92986 C92987 Dose Level: 0 30 10 30 10 10 10 10 000- Dose Level: 0.5 10 0010 10 010 30 10 10 - Dosage Unit: ppm T-6295 --------------------- Dosage Unit: ppm T-6295 ----+ ----------------- - - Covance 6329-183 3M T-6295 1682 Appendix 5 Individual Clinical Urinalysis Data Females Week 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER U PRO MG/DL U GLU U KET U BILI U BLOOD UROBILI Group: 3 C92992 C92993 C93000 C93018 C93019 C93023 C93024 C93026 C93030 C93035 Group: 4 C93051 C93064 C93071 C93076 C93078 C93085 C93089 C93090 C93093 C93109 Dose Level: 2.0 0 10 10 10 010 10 30 30 10 - Dose Level: 5.0 10 000000000- Dosage Unit: ppm T-6295 ------------------- Dosage Unit: ppm T-6295 - ---------------------------- Covance 6329-183 3M T-6295 1683 Appendix 5 Individual Clinical Urinalysis Data Females Week 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER U PRO MG/DL U GLU U KET U BILI U BLOOD UROBILI Group: 5 C93111 C93113 C93120 C93127 C93135 C93143 C93155 C93162 C93169 C93178 Dose Level: 20.0 0010 0010 10 010 0- Dosage Unit: ppm T-6295 --------------------- - Covance 6329-183 3M T-6295 1684 Appendix 5 Individual Clinical Urinalysis Data Females Week 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER RBC WBC EPITH BACT CASTS CRYSTALS PER HPF PER. HPF PER HPF PER HPF PER LPF PER LPF1 URINE COMMENTS APP1 Group: 1 C92863 C92865 C92871 QS C92880 C92887 C92898 C92903 C92905 C92906 C92927 Group: 2 C92935 C92940 C92944 C92948 C92956 C92962 C92967 C92978 C92986 C92987 Dose Level: 0 10 00 -00 00 00 00 00 00 00 Dose Level: 0.5 01 00 00 00 00 00 00 00 00 00 Dosage Unit:: ppm T-6295 01 0 110 --- 02 0 110 110 120 110 01 0 110 Dosage Unit: ppm T-6295 120 03 0 01 0 01 0 120 01 0 110 110 120 01 0 0 0 0 0 1D 2D 1D 0 0 4D 3B4D 0 1B1D 2D 0 1D 1D 4D 4D CK CJ DJ BJ BJ BJ BJ AJ AJ AJ CJ CJ AJ CJ CJ AJ BJ BJ CJ BJ S P P - P P P P P P - Covance 6329-183 3M T-6295 1685 Appendix 5 Individual Clinical Urinalysis Data Females Week 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER RBC WBC EPITH BACT CASTS CRYSTALS PER HPF PER HPF PER HPF PER HPF PER LPF PER LPF1 URINE COMMENTS APP1 Group: 3 C92992 C92993 C93000 C93018 C93019 C93023 C93024 C93026 C93030 C93035 Group: 4 C93051 C93064 C93071 C93076 C93078 C93085 C93089 C93090 C93093 C93109 Dose Level: 2.0 00 00 00 00 01 00 00 01 00 01 Dose Level: 5.0 00 00 01 00 00 00 00 01 00 00 Dosage Unit: ppm T-6295 110 1 30 110 120 110 110 110 120 110 110 Dosage Unit: ppm T-6295 11 01 11 11 11 11 01 11 01 01 0 0 0 0 0 0 0 0 0 0 0 4B 3D 4D 1B1D 1D 1D 2D 2D 4D 1D 1B 0 0 0 0 4D 1D 1D 0 AJ BJ BJ BJ BJ CJ CJ CK CJ BJ BJ BJ AJ AJ AJ BJ BJ AJ BK BJ P P P P P P P P - Covance 6329-183 3M T-6295 1686 Appendix 5 Individual Clinical Urinalysis Data Females Week 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER RBC WBC EPITH BACT CASTS CRYSTALS PER HPF PER HPF PER HPF PER HPF PER LPF PER LPF1 URINE COMMENTS APP1 Group: 5 Dose Level: 20.0 Dosage: Unit: ppm T-6295 C93111 0 0 1 1 0 4D BJ P C93113 0 0 0 1 0 0 BJ P C93120 0 0 0 1 0 4D CJ - C93127 0 1 01 0 0 AJ - C93135 0 0 0 3 0 0 AJ - C93143 0 0 1 1 0 4D BJ P C93155 0 0 1 1 0 4D CJ P C93162 0 1 1 2 0 2B BJ P C93169 0 0 1 1 0 0 BJ - C93178 0 0 1 1 0 0 AJ - Covance 6329-183 3M T-6295 1687 Appendix 5 Individual Clinical Urinalysis Data Males Week 27 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER U VOL ML SP GR U PH Group: 1 C92505 C92510 C92512 C92525 C92537 C92543 C92545 C92563 C92567 C92570 MEAN S.D. N Group: 2 C92577 C92583 C92585 C92586 C92588 C92590 C92601 C92602 C92617 C92630 MEAN S.D. N Dose Level: 0 4.5 4.6 16.2 19.0 15.4 18.4 22.2 7.5 23.5 20.4 15.2 7.12 10 1.028 1.047 1.017 1.017 1.022 1.016 1.014 1.031 1.014 1.016 1.022 .0105 10 Dose Level: 0.5 12.2 10.6 10.6 13.0 14.6 28.2 10.0 17.8 11.0 34.5 1.019 1.018 1.024 1.020 1.016 1.010 1.021 1.012 1.025 1.009 16.2 8.42 10 1.017 .0056 10 Dosage Unit ppm T-6295 6.5 6.5 6.5 7.5 7.0 7.0 7.0 7.0 7.0 7.0 6.9 .32 10 Dosage Unit ppm T-6295 7.0 7.0 7.0 7.0 7.5 7.0 7.0 7.5 7.0 7.0 7.1 .21 10 Covance 6329-183 3M T-6295 1688 Appendix 5 Individual Clinical Urinalysis Data Males Week 27 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER U VOL ML SP GR U PH Group: 3 C92633 C92644 C92647 C92658 C92660 C92667 C92673 C92679 C92686 C92687 MEAN S.D. N Group: 4 C92696 C92698 C92708 C92709 C92710 C92725 C92743 C92745 C92749 C92750 MEAN S.D. N Dose Level: 2.0 17.1 10.6 30.5 11.5 9.2 21.9 10.4 17.4 12.0 20.9 1.021 1.026 1.010 1.022 1.024 1.013 1.024 1.015 1.019 1.017 16.2 6.79 10 1.019 .0053 10 Dose Level: 5.0 16.1 47.7 a 11.2 23.0 8.6 14.5 20.2 8.1 5.6 49.0 a 1.017 1.004 1.024 1.015 1.030 1.013 1.015 1.039 1.024 1.006 20.4 15.70 10 1.019 .0107 10 Dosage Unit: ppm T-6295 7.0 7.0 6.5 7.5 7.0 7.5 6.5 7.0 7.0 6.5 7.0 .37 10 Dosage Unit: ppm T-6295 7.0 6.5 7.0 7.0 7.0 7.5 7.0 7.0 7.0 7.0 7.0 .24 10 a Sample was overflowing at the time of collection. 1689 Covance 6329-183 3M T-6295 Appendix 5 Individual Clinical Urinalysis Data Males Week 27 DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS;: T-6295) IN RATS ANIMAL NUMBER U VOL ML SP GR U PH Group: 5 C92756 C92761 C92764 C92773 C92783 C92788 C92791 C92803 C92804 C92820 MEAN S.D. N Dose Level: 20.0 16.5 7.0 1.5 168..81 47.7 a 10.4 19.5 4.9 46.8 a 1.022 1.027 1.086 1.019 1.038 1.007 1.030 1.014 1.037 1.006 17.9 16.42 10 1.029 .0231 10 Dosage Unit: ppm T-6295 7.0 6.5 6.5 7.0 6.5 7.0 7.0 7.0 7.0 6.5 6.8 .26 10 a Sample was overflowing at the time of collection. Covance 6329-183 3M T-6295 1690 Appendix 5 Individual Clinical Urinalysis Data Males Week 27 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER U PRO MG/DL U GLU U KET U BILI U BLOOD UROBILI Group: 1 C92505 C92510 C92512 C92525 C92537 C92543 C92545 C92563 C92567 C92570 Group: 2 C92577 C92583 C92585 C92586 C92588 C92590 C92601 C92602 C92617 C92630 Dose Level: 0 10 _ 30 30 _ 30 10 10 _ 10 300 10 100 - Dose Level: 0.5 10 10 100 10 10 10 10 10 30 100 - Dosage Unit: ppm T-6295 +-+ - +++ --+ - +++ ----+-----+-- Dosage Unit: ppm T-6295 --+---------+-------- - - Covance 6329-183 3M T-6295 1691 Appendix 5 Individual Clinical Urinalysis Data Males Week 27 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T- 6295) IN RATS ANIMAL NUMBER U PRO MG/DL U GLU U KET U BILI U BLOOD UROBILI Group: 3 C92633 C92644 C92647 C92658 C92660 C92667 C92673 C92679 C92686 C92687 Group: 4 C92696 C92698 C92708 C92709 C92710 C92725 C92743 C92745 C92749 C92750 Dose Level: 2.0 10 10 030 30 10 10 10 10 100 - Dose Level: 5.0 10 010 300 30 10 10 100 10 0- Dosage Unit: ppm T-6295 +-------+---+ --+---- --- Dosage Unit: ppm T-6295 +-- ++ -- +++++-- - -- - - - Covance 6329-183 3M T-6295 1692 Appendix 5 Individual Clinical Urinalysis Data Males Week 27 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER U PRO MG/DL U GLU U KET U BILI U BLOOD UROBILI Group: 5 C92756 C92761 C92764 C92773 C92783 C92788 C92791 C92803 C92804 C92820 Dose Level: 20.0 30 10 300 10 100 030 10 30 10 - Dosage Unit: ppm T-6295 + --+---+---+---+---- - Covance 6329-183 3M T-6295 1693 Appendix 5 Individual Clinical Urinalysis Data Males Week 27 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER RBC WBC EPITH BACT CASTS CRYSTALS PER HPF PER HPF PER HPF PER HPF PER LPF PER LPF1 URINE COMMENTS APP1 Group: 1 C92505 C92510 C92512 C92525 C92537 C92543 C92545 C92563 C92567 C92570 Group: 2 C92577 C92583 C92585 C92586 C92588 C92590 C92601 C92602 C92617 C92630 Dose Level: 0 01 40 00 10 01 00 00 01 00 00 Dose Level: 0.5 00 01 01 00 00 01 01 01 00 01 Dosage Unit: ppm T-6295 120 110 120 120 01 0 01 0 120 1 2 1G 1 30 120 Dosage Unit: ppm T-6295 120 120 02 0 120 1 30 110 120 120 110 110 3D 3D 0 3D 4D 4D 1B3D 1B1D 1D1E 1D 4D 4D 3D 2B2D 4D 1B1D 2B2D 4D 2D 1D BJ CK BJ BJ BJ BJ BJ BJ BJ BJ BJ BJ BJ BJ BJ AJ BJ BJ BJ AJ PQ Q PQ PQ Q PQ Q PQ Q Q PQ PQ PQ PQ PQ Q PQ PQ Q Q Covance 6329-183 3M T-6295 1694 Appendix 5 Individual Clinical Urinalysis Data Males Week 27 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER RBC WBC EPITH BACT CASTS CRYSTALS PER HPF PER HPF PER HPF PER HPF PER LPF PER LPF1 URINE COMMENTS APP1 Group: 3 C92633 C92644 C92647 C92658 C92660 C92667 C92673 C92679 C92686 C92687 Group: 4 C92696 C92698 C92708 C92709 C92710 C92725 C92743 C92745 C92749 C92750 Dose Level: 2.0 01 00 01 00 01 00 00 00 01 01 Dose Level: 5.0 00 00 00 00 00 00 01 01 00 00 Dosage Unit: ppm T-6295 120 1 30 120 120 120 02 0 01 0 110 120 1 2 1H Dosage Unit: ppm T-6295 120 110 110 120 1 30 120 120 120 02 0 110 2B2D 4D 1B1D 3D 4D 2B2D 1D 1B1D 1B1D 1B1D 4D 1D 3D 4D 4D 4D 1B1D 4D 4D 1D BJ BJ AJ BJ BJ BJ BJ BJ BJ BJ BJ AJ BJ BJ BJ BJ BJ BJ BJ AJ PQ PQ Q Q Q Q Q Q Q Q Q Q Q Q Q PQ Q PQ PQ Q Covance 6329-183 3M T-6295 1695 Appendix 5 Individual Clinical Urinalysis Data Males Week 27 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER RBC WBC EPITH BACT CASTS CRYSTALS PER HPF PER HPF PER HPF PER HPF PER LPF PER LPF1 URINE COMMENTS APP1 Group: 5 Dose Level: 20.0 Dosage Unit: ppm T-6295 C92756 0 0 1 3 0 3D BJ PQ C92761 1 01 2 0 0 BJ Q C92764 0 0 0 1 0 4D CK PQ C92773 0 0 0 2 0 4D BJ Q C92783 0 0 1 2 0 3D BJ PQ C92788 0 0 1 1 0 1B AJ Q C92791 0 0 0 2 0 2D BJ PQ C92803 0 0 1 1 0 2D BJ PQ C92804 0 1 1 2 0 2D BJ Q C92820 0 1 1 1 0 1D AJ Q Covance 6329-183 3M T-6295 1696 Appendix 5 Individual Clinical Urinalysis Data Females Week 27 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER U VOL ML SP GR U PH Group: 1 C92876 C92878 C92888 C92906 C92916 C92917 C92921 C92922 C92923 C92928 MEAN S.D. N Group: 2 C92932 C92935 C92938 C92951 C92954 C92956 C92960 C92974 C92979 C92989 MEAN S.D. N Dose Level: 0 1.8 10.5 7.0 26.4 13.6 5.2 47.4 a 47.0 a 13.1 4.0 1.046 1.014 1.022 1.004 1.018 1.031 1.002 1.002 1.012 1.006 17.6 17.05 10 1.016 .0142 10 Dose Level: 0.5 13.6 6.1 20.4 8.0 22.5 11.5 14.8 47.0 a 9.5 5.2 1.018 1.026 1.007 1.017 1.006 1.018 1.016 1.002 1.016 1.031 15.9 12.34 10 1.016 .0089 10 Dosage 6.5 6.5 6.5 6.5 7.0 6.5 6.5 6.5 6.5 7.0 6.6 .21 10 Dosage 6.5 7.0 6.5 7.0 6.5 6.5 7.0 6.5 7.0 6.0 6.6 .34 10 ppm T-6295 ppm T-6295 a Sample was overflowing at the time of collection. 1697 Covance 6329-183 3M T-6295 Appendix 5 Individual Clinical Urinalysis Data Females Week 27 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER U VOL ML SP GR U PH Group: 3 C93003 C93004 C93011 C93020 C93027 C93030 C93034 C93046 C93047 C93049 MEAN S.D. N Group: 4 C93052 C93055 C93072 C93080 C93081 C93090 C93094 C93095 C93103 C93104 MEAN S.D. N Dose Level: 2.0 42.2 3.4 21.7 9.1 12.8 4.0 6.9 19.5 29.7 6.2 1.006 1.024 1.009 1.019 1.012 1.034 1.019 1.008 1.006 1.021 15.6 12.72 10 1.016 .0092 10 Dose Level: 5.0 46.0 a 5.5 9.5 30.5 39.2 19.2 24.9 1.7 20.0 19.5 1.002 1.032 1.018 1.006 1.006 1.010 1.010 1.032 1.012 1.013 21.6 14.18 10 1.014 .0104 10 Dosage Unit: ppm T-6295 7.0 7.0 7.0 7.0 7.0 7.0 6.5 6.5 6.5 7.0 6.8 .24 10 Dosage Unit: ppm T-6295 6.5 6.5 7.0 7.0 7.0 7.5 7.0 7.0 7.0 6.5 6.9 .32 10 a Sample was overflowing at the time of collection. 1698 Covance 6329-183 3M T-6295 Appendix 5 Individual Clinical Urinalysis Data Females Week 27 DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER U VOL ML SP GR U PH Group: 5 C93119 C93131 C93133 C93138 C93149 C93153 C93157 C93160 C93162 C93179 MEAN S.D. N Dose Level: 20.0 24.4 5.8 1.2 13.7 11.5 11.2 40.9 9.0 15.8 3.9 13.7 11.58 10 1.005 1.025 1.062 1.011 1.013 1.017 1.006 1.022 1.018 1.036 1.022 .0170 10 Dosage Unit: ppm T-6295 6.5 6.5 6.5 6.5 7.0 7.0 6.0 7.0 6.0 6.5 6.6 .37 10 Covance 6329-183 3M T-6295 1699 Appendix 5 Individual Clinical Urinalysis Data Females Week 27 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER U PRO MG/DL U GLU U KET U BILI U BLOOD UROBILI Group: 1 C92876 C92878 C92888 C92906 C92916 C92917 C92921 C92922 C92923 C92928 Group: 2 C92932 C92935 C92938 C92951 C92954 C92956 C92960 C92974 C92979 C92989 Dose Level: 0 100 000100 10 0000- Dose Level: 0.5 0 10 010 000010 0- Dosage Unit: ppm T-6295 -+ --+ ----+ ------------- Dosage Unit: ppm T-6295 - ---------------------------- Covance 6329-183 3M T-6295 1700 Appendix 5 Individual Clinical Urinalysis Data Females Week 27 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER U PRO MG/DL U GLU U KET U BILI U BLOOD UROBILI Group: 3 C93003 C93004 C93011 C93020 C93027 C93030 C93034 C93046 C93047 C93049 Group: 4 C93052 C93055 C93072 C93080 C93081 C93090 C93094 C93095 C93103 C93104 Dose Level: 2.0 010 00010 0000- Dose Level: 5.0 0010 000010 00- Dosage Unit: ppm T-6295 --------------------- Dosage Unit: ppm T-6295 --------------------- - - Covance 6329-183 3M T-6295 1701 Appendix 5 Individual Clinical Urinalysis Data Females Week 27 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER U PRO MG/DL U GLU U KET U BILI U BLOOD UROBILI Group: 5 C93119 C93131 C93133 C93138 C93149 C93153 C93157 C93160 C93162 C93179 Dose Level: 20.0 010 30 000010 010 - Dosage Unit: ppm T-6295 --------------------- - Covance 6329-183 3M T-6295 1702 Appendix 5 Individual Clinical Urinalysis Data Females Week 27 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER RBC WBC EPITH BACT CASTS CRYSTALS PER HPF PER. HPF PER HPF PER HPF PER LPF PER LPF1 URINE COMMENTS APP1 Group: 1 0Dose Level: Dosage Unit:: ppm T-6295 C92876 C92878 C92888 C92906 C92916 C92917 C92921 C92922 C92923 C92928 0000000000 0000000000 1111000000 211111 31 1 1 0000000000 00 CJ P BJ P 10B BJ AJ P - 10D 0 0 0 0 BJ BJ AJ AJ BJ AJ P P P - 2Group: Dose Level: 0.5 Dosage Unit: ppm T-6295 C92932 C92935 C92938 C92951 C92954 C92956 C92960 C92974 C92979 C92989 0000000000 1110000000 1111111100 21111 31 2 2 1 0000000000 0 BJ P 30D BJ AJ P P 200B 0 0 BJ AJ BJ BJ AJ P P - 20D BJ BJ P P Covance 6329-183 3M T-6295 1703 Appendix 5 Individual Clinical Urinalysis Data Females Week 27 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER RBC WBC EPITH BACT CASTS CRYSTALS PER HPF PER: HPF PER HPF PER HPF PER LPF PER LPF1 URINE COMMENTS APP1 Group: 3 Dose Level: 2. 0 Dosage Unit:: ppm T-6295 C93003 0 0 1 1 0 2D AJ - C93004 0 0 1 1 0 0 BJ - C93011 0 0 1 1 0 3D AJ P C93020 0 0 0 1 0 0 BJ - C93027 0 1 1 1 0 0 AJ P C93030 0 0 1 3 0 4D BJ P C93034 0 1 02 0 2B BJ P C93046 0 0 1 2 0 0 AJ P C93047 0 0 1 2 0 0 AJ - C93049 0 0 1 1 0 4D BJ P Group: 4 Dose Level: 5.0 Dosage Unit: ppm T-6295 C93052 0 0 1 1 0 0 AJ - C93055 0 0 1 2 0 2B BJ - C93072 0 0 1 2 0 1B BJ PT C93080 0 0 1 1 0 0 AJ - C93081 0 0 1 1 0 0 AJ - C93090 0 0 1 2 0 3D AJ P C93094 0 0 0 1 0 3D AJ P C93095 QS - - - - - - BJ - C93103 0 0 1 2 0 3D BJ P C93104 0 0 1 2 0 1B BJ P Covance 6329-183 3M T-6295 1704 Appendix 5 Individual Clinical Urinalysis Data Females Week 27 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER RBC WBC EPITH BACT CASTS CRYSTALS PER HPF PER HPF PER HPF PER HPF PER LPF PER LPF1 URINE COMMENTS APP1 Group: 5 Dose Level: 20.0 Dosage: Unit: ppm T-6295 C93119 0 0 1 1 0 0 AJ - C93131 0 0 0 2 0 1B BJ P C93133 0 0 1 1 0 4D CJ P C93138 0 0 1 1 0 0 AJ P C93149 0 0 1 2 0 0 BJ P C93153 0 0 1 1 0 0 BJ P C93157 0 0 0 1 0 0 AJ - C93160 0 0 1 1 0 0 BJ P C93162 0 0 1 2 0 2B BJ - C93179 0 0 1 1 0 0 BJ P Covance 6329-183 3M T-6295 1705 Appendix 5 Individual Clinical Urinalysis Data Males Week 53 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER U VOL ML SP GR U PH Group: 1 C92501 C92504 C92507 C92512 C92517 C92523 C92534 C92540 C92562 C92570 MEAN S.D. N Group: 2 C92573 C92587 C92592 C92596 C92604 C92612 C92614 C92624 C92625 C92629 MEAN S.D. N Dose Level: 0 13.3 19.0 30.5 9.6 6.5 6.8 4.6 9.0 9.5 16.2 1.030 1.014 1.007 1.021 1.043 1.028 1.038 1.029 1.023 1.016 12.5 7.75 10 1.025 .0110 10 Dose Level: 0.5 10.2 19.0 9.6 15.8 28.0 16.0 2.1 48.0 a 6.7 24.8 1.034 1.024 1.030 1.023 1.015 1.020 1.050 1.011 1.041 1.017 18.0 13.20 10 1.026 .0123 10 Dosage Unit: ppm T-6295 7.0 6.5 7.0 6.5 6.5 6.5 6.0 6.5 6.5 7.0 6.6 .32 10 Dosage Unit: ppm T-6295 6.5 7.0 6.5 7.0 7.0 7.0 6.5 6.5 6.5 6.5 6.7 .26 10 a Sample was overflowing at the time of collection. 1706 Covance 6329-183 3M T-6295 Appendix 5 Individual Clinical Urinalysis Data Males Week 53 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER U VOL ML SP GR U PH Group: 3 C92634 C92635 C92643 C92649 C92653 C92654 C92667 C92669 C92680 C92686 MEAN S.D. N Group: 4 C92694 C92704 C92710 C92711 C92727 C92729 C92739 C92741 C92742 C92748 MEAN S.D. N Dose Level: 2. 0 8.7 11.2 10.1 16.2 24.5 30.0 26.5 14.5 10.2 10.0 16.2 7.90 10 1.026 1.021 1.019 1.013 1.018 1.014 1.015 1.023 1.030 1.030 1.021 .0063 10 Dose Level: 5.0 28.5 4.0 8.5 9.0 12.6 11.0 8.2 11.4 3.2 19.8 1.011 1.051 1.025 1.028 1.025 1.025 1.034 1.021 1.074 1.021 11.6 7.53 10 1.032 .0182 10 Dosage Unit ppm T-6295 6.5 6.5 7.0 7.0 7.5 7.5 7.0 7.5 7.5 CO 7.1 .42 9 Dosage Unit ppm T-6295 6.5 7.0 7.0 7.5 6.5 7.0 6.5 7.5 6.5 7.0 6.9 .39 10 Covance 6329-183 3M T-6295 1707 Appendix 5 Individual Clinical Urinalysis Data Males Week 53 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER U VOL ML SP GR U PH Group: 5 C92751 C92754 C92755 C92778 C92790 C92795 C92796 C92817 C92820 MEAN S.D. N Dose Level: 20.0 9.4 11.0 3.0 12.2 6.7 17.4 16.0 9.2 14.2 1.032 1.023 1.038 1.026 1.037 1.020 1.020 1.033 1.020 11.0 4.55 9 1.028 .0074 9 Dosage 7.0 7.5 6.0 7.0 7.0 6.5 6.5 7.0 7.5 6.9 .49 9 ppm T-6295 Covance 6329-183 3M T-6295 1708 Appendix 5 Individual Clinical Urinalysis Data Males Week 53 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T- 6295) IN RATS ANIMAL NUMBER U PRO MG/DL U GLU U KET U BILI U BLOOD UROBILI Group: 1 C92501 C92504 C92507 C92512 C92517 C92523 C92534 C92540 C92562 C92570 Group: 2 C92573 C92587 C92592 C92596 C92604 C92612 C92614 C92624 C92625 C92629 Dose Level: 0 300 100 100 100 300 100 100 300 30 300 - Dose Level: 0.5 300 300 100 100 30 30 300 300 300 300 - Dosage Unit: ppm T-6295 ----------- - +++ --------- Dosage Unit: ppm T-6295 --------------------- - - Covance 6329-183 3M T-6295 1709 Appendix 5 Individual Clinical Urinalysis Data Males Week 53 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER U PRO MG/DL U GLU U KET U BILI U BLOOD UROBILI Group: 3 C92634 C92635 C92643 C92649 C92653 C92654 C92667 C92669 C92680 C92686 Group: 4 C92694 C92704 C92710 C92711 C92727 C92729 C92739 C92741 C92742 C92748 Dose Level: 2. 0 100 _ 30 30 _ 10 100 30 _ 100 30 300 CO - Dose Level: 5.0 10 100 30 100 300 300 30 30 300 300 - Dosage Unit: ppm T-6295 ------------- - +++ ----CO CO +++ Dosage Unit: ppm T-6295 --- - ++ ----------------- CO - Covance 6329-183 3M T-6295 1710 Appendix 5 Individual Clinical Urinalysis Data Males Week 53 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER U PRO MG/DL U GLU U KET U BILI U BLOOD UROBILI Group: 5 C92751 C92754 C92755 C92778 C92790 C92795 C92796 C92817 C92820 Dose Level: 20.0 30 30 100 100 300 30 30 30 30 - Dosage Unit: ppm T-6295 +---+ --+---------- - Covance 6329-183 3M T-6295 1711 Appendix 5 Individual Clinical Urinalysis Data Males Week 53 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T- 6295) IN RATS ANIMAL NUMBER RBC WBC EPITH BACT CASTS CRYSTALS PER HPF PER HPF PER HPF PER HPF PER LPF PER LPF1 URINE APP1 Group: 1 C92501 C92504 C92507 C92512 C92517 C92523 C92534 C92540 C92562 C92570 Group: 2 C92573 C92587 C92592 C92596 C92604 C92612 C92614 C92624 C92625 C92629 Dose Level: 0 00 11 00 00 00 00 00 01 00 01 Dose Level: 0.5 00 00 00 01 01 00 00 00 01 01 Dosage Unit: ppm T-6295 1 3 1G 110 02 0 110 1 30 120 110 1 3 1G 110 110 Dosage Unit: ppm T-6295 0 2 1G 01 0 1 30 120 01 0 02 0 02 0 0 2 1G1H 1 2 1G 1 2 1G1I 1D 0 0 0 4D 4D 0 4D 4D 1B 0 0 4D 1B 1B1D 2D 4D 0 1B1D 2B BJ BJ AJ BJ CJ BJ BJ BJ BJ BJ CK CK CJ CK BJ CK CJ AJ CK BK P P P PQ PQ PQ PQ P P P PQ P P Q PQ P PQ PQ Covance 6329-183 3M T-6295 1712 Appendix 5 Individual Clinical Urinalysis Data Males Week 53 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER RBC WBC EPITH BACT CASTS CRYSTALS PER HPF PER HPF PER HPF PER HPF PER LPF PER LPF1 URINE COMMENTS APP1 Group: 3 C92634 C92635 C92643 C92649 C92653 C92654 C92667 C92669 C92680 C92686 Group: 4 C92694 C92704 C92710 C92711 C92727 C92729 C92739 C92741 C92742 C92748 Dose Level: 2.0 00 01 00 00 00 00 10 00 00 40 Dose Level: 5.0 00 10 00 00 00 00 01 00 00 00 Dosage Unit: ppm T-6295 110 01 0 110 120 02 0 120 02 0 110 120 000 Dosage Unit: ppm T-6295 01 0 110 01 0 120 110 120 120 120 110 110 0 1B 4D 0 4D 4D 0 4D 4D 0 0 2D 1B 4D 0 2B4D 1B1D 4D 3D 2D CK CK CK BJ BK BK BK CK CK GL BJ DK CK CK CK BJ CK BJ DK CK PQ PQ PQ PQ Q PQ Q PQ PQ - PQ PQ PQ PQ PQ PQ PS P Q PQ Covance 6329-183 3M T-6295 1713 Appendix 5 Individual Clinical Urinalysis Data Males Week 53 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER RBC WBC EPITH BACT CASTS CRYSTALS PER HPF PER HPF PER HPF PER HPF PER LPF PER LPF1 URINE COMMENTS APP1 Group: 5 C92751 C92754 C92755 C92778 C92790 C92795 C92796 C92817 C92820 Dose Level: 20.0 00 00 00 00 01 00 01 00 02 Dosage Unit: ppm T-6295 1 30 120 1 30 110 02 0 120 110 110 02 0 4D 4D 0 4D 2B3D 0 2B 4D 1B3D CJ BJ CJ BJ CK BJ BJ CJ BJ PQ P P P PQ P PQ P PQ Covance 6329-183 3M T-6295 1714 Appendix 5 Individual Clinical Urinalysis Data Females Week 53 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-62 ANIMAL NUMBER U VOL ML SP GR U PH Group: 1 C92862 C92876 C92889 C92894 C92899 C92910 C92917 C92922 C92923 C92930 MEAN S.D. N Group: 2 C92931 C92938 C92946 C92952 C92954 C92958 C92964 C92972 C92985 C92990 MEAN S.D. N Dose Level: 0 6.8 8. 0 10.6 8.5 7.8 9.0 14.0 8.9 8. 0 9.4 9.1 2. 00 10 1.023 1.028 1.019 1.021 1.026 1.025 1.014 1.022 1.021 1.024 1.022 .0039 10 Dose Level: 0.5 15.3 3.0 5.7 9.5 4.7 6.5 3.8 10.7 5.7 12.5 1.020 1.036 1.025 1.020 1.030 1.026 1.035 1.016 1.025 1.017 7.7 4.07 10 1.025 .0070 10 Dossaagg<e Unit: ppm T-6295 6.0 6.5 7.0 6.5 6.5 6.0 6.5 7.0 6.5 6.5 6.5 .33 10 Dossaagg<e Unit: ppm T-6295 7.5 6.5 7.0 6.5 6.5 6.0 6.0 7.0 7.0 6.0 6.6 .52 10 Covance 6329-183 3M T-6295 1715 Appendix 5 Individual Clinical Urinalysis Data Females Week 53 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS;? T-6: ANIMAL NUMBER U VOL ML SP GR U PH Group: 3 C92992 C92997 C93012 C93022 C93027 C93029 C93032 C93042 C93046 C93048 MEAN S.D. N Group: 4 C93052 C93054 C93060 C93078 C93081 C93083 C93091 C93095 C93097 C93110 MEAN S.D. N Dose Level: 2.0 7.0 1.9 13.8 250..20 4.8 6.4 9.1 138..09 1.027 1.051 1.023 11..001224 1.034 1.023 11..001205 1.023 9.0 5.46 10 1.025 .0114 10 Dose Level: 5.0 23..83 5.7 28..25 43.5 4.2 1.5 4.2 7.1 1.040 1.036 1.023 1.033 1.019 1.008 1.028 1.041 1.037 1.034 8.3 12.1504 1.030 .011005 Dosage Unit: ppm T-6295 6.5 6.5 6.5 7.0 6.5 66..05 6.0 6.5 7.0 6.5 .3 10 Dosage Unit: ppm T-6295 6.5 6.5 66..05 7.5 7.0 6.5 6.5 6.5 7.0 6.6 1.04 Covance 6329-183 3M T-6295 1716 Appendix 5 Individual Clinical Urinalysis Data Females Week 53 DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER U VOL ML SP GR U PH Group: 5 C93112 C93116 C93117 C93120 C93130 C93138 C93147 C93154 C93163 C93171 MEAN S.D. N Dose Level: 20.0 8.7 6.2 7.3 6.5 9.8 47.5 a 4.3 2.5 3.8 4.0 10.1 13.35 10 1.025 1.024 1.021 1.024 1.022 1.004 1.035 1.043 1.036 1.042 1.028 .0117 10 Dosage Unit: ppm T-6295 6.0 6.0 6.5 6.0 6.5 6.5 6.5 6.0 6.5 6.0 6.2 .26 10 a Sample was overflowing at the time of collection. Covance 6329-183 3M T-6295 1717 Appendix 5 Individual Clinical Urinalysis Data Females Week 53 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER U PRO MG/DL U GLU U KET U BILI U BLOOD UROBILI Group: 1 C92862 C92876 C92889 C92894 C92899 C92910 C92917 C92922 C92923 C92930 Group: 2 C92931 C92938 C92946 C92952 C92954 C92958 C92964 C92972 C92985 C92990 Dose Level: 0 10 _ 100 10 _ 10 30 30 _ 100 30 10 300 - Dose Level: 0.5 10 100 30 30 30 30 100 10 300 10 - Dosage Unit: ppm T-6295 --------------+-+ ----- Dosage Unit: ppm T-6295 --+ ------------------- - - Covance 6329-183 3M T-6295 1718 Appendix 5 Individual Clinical Urinalysis Data Females Week 53 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T- 6295) IN RATS ANIMAL NUMBER U PRO MG/DL U GLU U KET U BILI U BLOOD UROBILI Group: 3 C92992 C92997 C93012 C93022 C93027 C93029 C93032 C93042 C93046 C93048 Group: 4 C93052 C93054 C93060 C93078 C93081 C93083 C93091 C93095 C93097 C93110 Dose Level: 2.0 30 100 10 30 10 30 10 30 300 10 - Dose Level: 5.0 30 30 10 30 10 10 30 30 30 300 - Dosage Unit: ppm T-6295 ----------+ ----------- Dosage Unit: ppm T-6295 --------------------- - - Covance 6329-183 3M T-6295 1719 Appendix 5 Individual Clinical Urinalysis Data Females Week 53 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER U PRO MG/DL U GLU U KET U BILI U BLOOD UROBILI Group: 5 C93112 C93116 C93117 C93120 C93130 C93138 C93147 C93154 C93163 C93171 Dose Level: 20.0 30 10 10 30 10 010 30 30 30 - Dosage Unit: ppm T-6295 ----------+ --------- - Covance 6329-183 3M T-6295 1720 Appendix 5 Individual Clinical Urinalysis Data Females Week 53 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER RBC WBC EPITH BACT CASTS CRYSTALS PER HPF PER. HPF PER HPF PER HPF PER LPF PER LPF1 URINE COMMENTS APP1 Group: 1 C92862 C92876 C92889 C92894 C92899 C92910 C92917 C92922 C92923 C92930 Group: 2 C92931 C92938 C92946 C92952 C92954 C92958 C92964 C92972 C92985 C92990 Dose Level: 0 02 01 01 00 01 00 01 40 01 01 Dose Level: 0.5 30 01 11 00 00 00 40 00 01 01 Dosage Unit:: ppm T-6295 110 110 110 110 110 230 01 0 120 110 01 0 Dosage Unit: ppm T-6295 120 03 0 110 110 01 0 01 0 01 0 01 0 120 01 0 0 0 0 0 0 0 1D 4D1E 0 1B 4D 0 3D 0 1B 0 0 1B 1B1D 1B BJ BJ BJ BJ BJ BJ BJ CK BJ BJ CK CK CK BK CK CJ GL BK CK CK P P P P P P P - P P P P S P - Covance 6329-183 3M T-6295 1721 Appendix 5 Individual Clinical Urinalysis Data Females Week 53 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER RBC WBC EPITH BACT CASTS CRYSTALS PER HPF PER HPF PER HPF PER HPF PER LPF PER LPF1 URINE COMMENTS APP1 Group: 3 C92992 C92997 C93012 C93022 C93027 C93029 C93032 C93042 C93046 C93048 Group: 4 C93052 C93054 C93060 C93078 C93081 C93083 C93091 C93095 C93097 C93110 Dose Level: 2.0 00 00 01 00 01 00 00 00 00 00 Dose Level: 5.0 00 01 01 00 00 00 00 01 01 01 Dosage Unit: ppm T-6295 110 1 30 120 110 110 01 0 1 1 1G 01 0 110 1 30 Dosage Unit: ppm T-6295 01 0 110 1 1 1G 01 0 110 120 1 3 1G 110 01 0 1 2 1G 0 0 2B2D 0 0 0 0 1B1D 1B 4D 0 0 0 1D 4D 1B2D 0 0 1B1D 2B2D CK DJ CK CJ BJ CK BJ CK BJ BJ CJ BJ BJ CJ BJ AJ CJ CJ CJ CK P P P P P P P P P P P P P P P P P P Covance 6329-183 3M T-6295 1722 Appendix 5 Individual Clinical Urinalysis Data Females Week 53 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER RBC WBC EPITH BACT CASTS CRYSTALS PER HPF PER HPF PER HPF PER HPF PER LPF PER LPF1 URINE COMMENTS APP1 Group: 5 C93112 C93116 C93117 C93120 C93130 C93138 C93147 C93154 C93163 C93171 Dose Level: 20.0 00 00 10 01 00 00 01 00 01 00 Dosage: Unit: ppm T-6295 110 120 1 30 110 02 0 02 0 110 110 110 110 1E 0 1D 3E 0 0 1B1D 3E 1B 3E BJ BJ CJ CJ BJ AJ CJ BJ CJ BJ P P P P - P P - P Covance 6329-183 3M T-6295 1723 Appendix 5 Individual Urine Chemistry Data Males Week 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER U NA MMOL/L UK MMOL/L NA EXC MMOL K EXC MMOL Group: 1 C92505 C92506 C92524 a C92526 C92529 C92542 C92543 C92546 C92548 C92553 MEAN S.D. N Group: 2 C92573 C92574 C92575 C92585 C92586 a C92587 C92610 C92613 C92615 C92618 MEAN S.D. N Dose Level: 0 12 80 12 50 7 28 8 48 9 57 12 73 11 71 19 78 13 100 15 61 12 3.5 10 65 2 0.2 10 Dose Level: 0. 5 23 13 22 12 8 28 5 52 21 7 19 13.9 10 111 111 113 75 19 61 27 192 114 32 86 53. 4 10 Dosage Unit: ppm .14 .92 .41 1.71 -- .18 1.08 .16 1.00 .23 1.40 .19 1.24 .31 1.29 .12 .95 .25 1.02 .22 .092 9 1.18 .258 9 Dosage Unit: ppm .30 1.47 .19 1.60 .27 1.38 .21 1.31 -.51 1 . 1 2 .21 1.15 .33 1.23 .30 1.65 .27 1.23 .29 .096 9 1.35 .191 9 a Unable to calculate due to inaccurate urine volume. 1724 Covance 6329-183 3M T-6295 Appendix 5 Individual Urine Chemistry Data Males Week 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER U NA MMOL/L UK MMOL/L NA EXC MMOL K EXC MMOL Group: 3 C92632 C92646 a C92650 a C92652 C92657 a C92668 C92676 C92678 C92684 C92686 MEAN S.D. N Group: 4 C92709 C92715 C92716 a C92718 a C92725 C92731 a C92732 C92734 C92744 C92746 MEAN S.D. N Dose Level: 2. 0 20 139 3 23 3 11 7 54 7 22 11 59 11 60 18 124 34 64 16 90 13 9.5 10 65 42. 5 10 Dose Level: 5. 0 18 89 22 60 7 25 4 15 10 49 6 21 24 48 16 119 7 45 24 66 14 7.9 10 54 32. 0 10 Dosage Unit: ppm .21 1.43 --- .14 1.09 -- .26 1.37 .18 .97 .17 1.15 .48 .90 .24 1.37 .24 .114 7 1.18 .211 7 Dosage Unit: ppm .28 1.38 .60 1.64 -- -.24 1.20 -- .56 1.13 .18 1.33 .30 1.93 .61 1. 68 .40 .186 7 1.47 .289 7 a Unable to calculate due to inaccurate urine volume. 1725 Covance 6329-183 3M T-6295 Appendix 5 Individual Urine Chemistry Data Males Week 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER U NA MMOL/L UK MMOL/L NA EXC MMOL K EXC MMOL Group: 5 C92752 C92759 C92779 C92784 C92793 C92798 C92799 C92805 C92807 C92811 MEAN S.D. N Dose Level: 20.0 6 40 8 56 62 163 14 49 11 87 12 50 9 29 11 66 22 95 14 46 17 16.4 10 68 39.1 10 Dosage Unit: ppm T-6295 17 1.16 19 1.34 37 .98 37 1.31 19 1.48 34 1.43 36 1.15 22 1.31 22 .97 35 1.14 .28 .086 10 1.23 .175 10 Covance 6329-183 3M T-6295 1726 Appendix 5 Individual Urine Chemistry Data Females Week 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER U NA MMOL/L UK MMOL/L NA EXC MMOL K EXC MMOL Group: 1 C92861 C92864 C92876 C92877 C92882 C92883 C92914 C92919 a C92925 C92926 MEAN S.D. N Group: 2 C92941 C92945 a C92950 C92953 C92959 C92964 C92968 C92969 C92976 a C92982 MEAN S.D. N Dose Level: 0 19 31 32 27 21 8 39 5 18 25 22 10.6 10 90 148 212 90 81 41 160 19 56 137 103 59. 9 10 Dose Level: 0. 5 64 265 6 11 6 42 24 116 7 42 29 94 29 78 35 126 9 15 21 73 23 18.0 10 86 74. 1 10 Dosage Unit: ppm .17 .81 . 1 1 .50 .07 .47 .18 .59 .13 .52 .18 .92 . 1 1 .43 -- .17 .53 .05 .26 .13 .049 9 .56 .198 9 Dosage Unit: ppm .10 .40 -- .09 .65 .16 .78 .07 .42 .21 .68 .28 .75 .12 .44 -- .18 .62 .15 .071 8 .59 .152 8 a Unable to calculate due to inaccurate urine volume. 1727 Covance 6329-183 3M T-6295 Appendix 5 Individual Urine Chemistry Data Females Week 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER U NA MMOL/L UK MMOL/L NA EXC MMOL K EXC MMOL Group: 3 C92993 C92996 a C92998 C93004 C93006 C93008 C93023 a C93031 C93033 C93045 MEAN S.D. N Group: 4 C93058 C93067 C93070 C93074 C93075 C93083 C93084 C93089 C93094 C93105 MEAN S.D. N Dose Level: 2. 0 46 106 4 11 15 63 25 118 24 60 33 78 47 51 126 42 112 38 116 28 16.7 10 80 43. 9 10 Dose Level: 5. 0 26 106 12 52 32 96 42 123 39 84 11 33 11 30 24 91 10 36 15 46 22 12.2 10 70 34. 1 10 Dosage Unit: ppm .18 - .08 .16 .14 .25 .21 .27 .28 .20 .069 8 .42 - .32 .73 .35 .60 .52 .72 .87 .57 .198 8 Dosage Unit: ppm .17 .69 .17 .73 .17 .50 .34 .98 .35 .76 .20 .60 .14 .38 .27 1.02 .29 1.04 .27 .83 .24 .076 10 .75 .222 10 a Unable to calculate due to inaccurate urine volume. 1728 Covance 6329-183 3M T-6295 Appendix 5 Individual Urine Chemistry Data Females Week 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER U NA MMOL/L UK MMOL/L NA EXC MMOL K EXC MMOL Group: 5 C93114 C93123 C93142 C93144 C93151 C93155 C93158 a C93159 a C93164 C93175 MEAN S.D. N Dose Level : 20.0 8 32 16 64 26 59 17 70 25 97 418 141 17 5 13 14 71 37 105 20 67 12.3 40.2 10 10 Dosage Unit: pp' .23 .15 .25 .17 .13 .17 .14 .24 .18 .048 8 .92 .61 .58 .70 .49 .58 - ..6782 .66 .130 8 a Unable to calculate due to inaccurate urine volume. Covance 6329-183 3M T-6295 1729 Appendix 5 Individual Urine Chemistry Data Males Week 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER U NA MMOL/L UK MMOL/L NA EXC MMOL K EXC MMOL Group: 1 C92508 C92509 C92511 C92521 C92527 C92528 a C92532 C92535 C92537 a C92562 a MEAN S.D. N Group: 2 C92583 C92593 a C92598 C92600 C92612 a C92616 C92621 C92624 C92627 C92629 MEAN S.D. N Dose Level: 0 7 17 23 59 13 8 38 19 6 6 20 17.1 10 29 81 43 129 43 16 70 159 12 19 60 50. 1 10 Dose Level: 0. 5 17 64 7 16 13 45 38 123 7 21 17 35 57 214 31 69 19 38 41 242 25 16.5 10 87 80. 7 10 Dosage Unit: ppp'm T-6295 .21 .88 .32 1.55 .72 1.35 .54 1.17 .34 1 . 1 2 -- .76 1.40 .09 .72 -- -- .43 .255 7 1.17 .294 7 Dosage Unit: pp .29 1.08 -- .31 1.06 .28 .91 -- .49 1.00 .19 .73 .53 1.17 .68 1.35 .30 1.77 .38 .165 8 1.13 .314 8 a Unable to calculate due to inaccurate urine volume. 1730 Covance 6329-183 3M T-6295 Appendix 5 Individual Urine Chemistry Data Males Week 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER U NA MMOL/L UK MMOL/L NA EXC MMOL K EXC MMOL Group: 3 C92636 a C92640 C92644 C92645 C92651 C92661 C92662 C92676 C92682 C92684 a MEAN S.D. N Group: 4 C92711 C92713 a C92714 C92716 C92719 C92722 C92726 C92728 C92741 a C92748 MEAN S.D. N Dose Level: 2. 0 3 11 20 102 28 119 49 95 24 107 15 33 32 90 24 194 20 56 11 12 23 12.5 10 82 56. 0 10 Dose Level: 5. 0 28 128 11 19 21 59 16 64 45 74 17 40 32 94 30 93 6 16 14 60 22 11.7 10 65 34. 8 10 Dosage Unit: ppm - .14 .26 .48 .36 .61 .32 .05 .41 - .33 .180 8 - .69 1.12 .92 1.58 1.34 .91 .39 1.15 - 1.01 .372 8 Dosage Unit: ppm .28 1.29 -- .46 1.30 .42 1. 68 .54 .89 .60 1.40 .41 1.20 .46 1.44 -- .33 1.41 .44 .104 8 1.33 .226 8 a Unable to calculate due to inaccurate urine volume. 1731 Covance 6329-183 3M T-6295 Appendix 5 Individual Urine Chemistry Data Males Week 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER U NA MMOL/L UK MMOL/L NA EXC MMOL K EXC MMOL Group: 5 C92754 C92765 C92767 C92774 C92777 C92784 C92789 C92799 C92812 C92820 MEAN S.D. N Dose Level: 20.0 12 102 41 127 12 41 27 79 7 30 35 96 23 74 34 125 47 146 25 66 26 13.2 10 89 37.8 10 Dosage Unit: ppm T-6295 11 .95 40 1.24 44 1.52 34 .99 34 1.44 42 1.15 13 .41 43 1.59 36 1 . 1 1 77 2.03 .37 .182 10 1.24 .438 10 Covance 6329-183 3M T-6295 1732 Appendix 5 Individual Urine Chemistry Data Females Week 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER U NA MMOL/L UK MMOL/L NA EXC MMOL K EXC MMOL Group: 1 C92863 C92865 C92871 QS C92880 C92887 C92898 C92903 C92905 a C92906 a C92927 a MEAN S.D. N Group: 2 C92935 C92940 C92944 a C92948 C92956 C92962 a C92967 C92978 C92986 C92987 MEAN S.D. N Dose Level: 0 60 138 37 118 -- 65 135 37 84 34 99 43 87 6 12 5 13 38 32 23.2 9 77 53. 0 9 Dose Level: 0. 5 65 142 9 20 11 16 28 66 21 52 13 20 32 101 27 87 16 82 47 118 27 17.6 10 70 43. 6 10 Dosage Unit: pp' .26 .26 .15 .23 .15 .28 - - .22 .058 6 .59 .83 .31 .52 .45 .57 - - .54 .172 6 Dosage Unit: pp .21 .45 .17 .37 -.20 .46 .26 .64 -.22 .70 .31 1.00 .10 .53 .15 .37 .20 .065 8 .56 .212 8 a Unable to calculate due to inaccurate urine volume. 1733 Covance 6329-183 3M T-6295 Appendix 5 Individual Urine Chemistry Data Females Week 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER U NA MMOL/L UK MMOL/L NA EXC MMOL K EXC MMOL Group: 3 C92992 C92993 C93000 C93018 C93019 C93023 C93024 C93026 C93030 C93035 MEAN S.D. N Group: 4 C93051 C93064 C93071 a C93076 C93078 a C93085 C93089 C93090 C93093 C93109 MEAN S.D. N Dose Level: 2.0 9 40 66 114 35 123 49 73 25 54 39 91 21 94 48 168 72 171 67 129 43 2 1 ..2 10 106 44. 1 10 Dose Level: 5.0 31 89 23 46 6 12 17 43 7 11 26 56 22 72 7 23 10 29 10 42 16 9..1 10 42 25. 2 10 Dosage Unit: ppi .17 .75 .24 .41 .18 .62 .39 .58 .51 1 . 1 0 .16 .38 .10 .47 .16 .57 .19 .46 .48 .92 .26 .146 10 .63 .234 10 Dosage Unit: ppm T-6295 .27 .77 .37 .74 -- .33 .84 -- .31 .68 .47 1.55 .29 .95 .26 .75 .18 .78 .31 .085 8 .88 .281 8 a Unable to calculate due to inaccurate urine volume. 1734 Covance 6329-183 3M T-6295 Appendix 5 Individual Urine Chemistry Data Females Week 14 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER U NA MMOL/L UK MMOL/L NA EXC MMOL K EXC MMOL Group: 5 C93111 C93113 C93120 C93127 C93135 a C93143 C93155 C93162 C93169 C93178 a MEAN S.D. N Dose Level : 20.0 36 58 13 43 46 66 20 32 6 16 50 86 143 252 20 50 31 81 4 10 37 40.5 10 69 6 8..9 10 Dosage Unit: ppi .49 .78 .16 .52 .37 .53 .76 1 . 22 -- .30 .52 .21 .38 .26 .66 .22 .58 -- .35 .197 8 .65 .259 8 a Unable to calculate due to inaccurate urine volume. Covance 6329-183 3M T-6295 1735 Appendix 5 Individual Urine Chemistry Data Males Week 27 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER U NA MMOL/L UK MMOL/L NA EXC MMOL K EXC MMOL Group: 1 C92505 C92510 C92512 C92525 C92537 C92543 C92545 C92563 C92567 C92570 MEAN S.D. N Group: 2 C92577 C92583 C92585 C92586 C92588 C92590 C92601 C92602 C92617 C92630 MEAN S.D. N Dose Level: 0 10 59 16 15 24 15 9 24 18 30 22 14.5 10 113 288 78 81 124 110 68 154 60 81 116 67. 1 10 Dose Level: 0. 5 16 96 18 76 16 113 13 89 12 62 8 46 9 71 16 55 42 124 7 40 16 10.0 10 77 28. 1 10 Dosage Unit: ppm .04 .51 .27 1.32 .26 1.26 .28 1.54 .37 1.91 .28 2.02 .20 1.51 .18 1.16 .42 1.41 .61 1.65 .29 .153 10 1.43 .422 10 Dosage Unit: ppm .20 1.17 .19 .81 .17 1.20 .17 1.16 .18 .91 .23 1.30 .09 .71 .28 .98 .46 1.36 .24 1.38 .22 .098 10 1.10 .234 10 Covance 6329-183 3M T-6295 1736 Appendix 5 Individual Urine Chemistry Data Males Week 27 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER U NA MMOL/L UK MMOL/L NA EXC MMOL K EXC MMOL Group: 3 C92633 C92644 C92647 TE C92658 C92660 C92667 C92673 C92679 C92686 C92687 MEAN S.D. N Group: 4 C92696 C92698 a C92708 C92709 C92710 C92725 C92743 C92745 C92749 C92750 a MEAN S.D. N Dose Level: 2.0 40 94 34 117 -- 28 110 25 125 18 53 18 105 14 59 10 75 30 95 24 9.9 9 93 25. 3 9 Dose Level: 5.0 22 62 2 11 24 113 17 63 26 158 13 56 17 70 60 228 19 110 9 20 21 15.5 10 89 65. 7 10 Dosage Unit: ppm .68 1.61 .36 1.24 -- .32 1.26 .23 1.15 .39 1.16 .19 1.09 .24 1.03 .12 .90 .63 1.99 .35 .192 9 1.27 .333 9 Dosage Unit: ppm .35 - .27 .39 .22 .19 .34 .49 .11 - .30 .122 8 1.00 - 1.27 1.45 1.36 .81 1.41 1.85 .62 - 1.22 .394 8 a Unable to calculate due to inaccurate urine volume. 1737 Covance 6329-183 3M T-6295 Appendix 5 Individual Urine Chemistry Data Males Week 27 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER U NA MMOL/L UK MMOL/L NA EXC MMOL K EXC MMOL Group: 5 C92756 C92761 C92764 C92773 C92783 C92788 a C92791 C92803 C92804 C92820 a MEAN S.D. N Dose Level : 20.0 23 136 38 116 142 414 30 91 67 187 6 25 83 153 13 58 47 192 6 18 46 42.4 10 139 114. 5 10 Dosage Unit: ppp'm T-6295 .38 2.24 .27 .81 .21 .62 .48 1.47 .59 1.65 -- .86 1.59 .25 1.13 .23 .94 -- .41 .226 8 1.31 .532 8 a Unable to calculate due to inaccurate urine volume. Covance 6329-183 3M T-6295 1738 Appendix 5 Individual Urine Chemistry Data Females Week 27 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER U NA MMOL/L UK MMOL/L NA EXC MMOL K EXC MMOL Group: 1 C92876 C92878 C92888 C92906 C92916 C92917 C92921 a C92922 a C92923 C92928 MEAN S.D. N Group: 2 C92932 C92935 C92938 C92951 C92954 C92956 C92960 C92974 a C92979 C92989 MEAN S.D. N Dose Level: 0 54 227 9 61 32 90 5 18 10 61 40 142 19 3 14 17 62 8 27 18 18.0 10 71 6 8 .2 10 Dose Level: 0. 5 17 75 35 154 8 30 12 70 5 22 13 80 9 71 2 14 25 66 19 163 14 9.9 10 74 50. 2 10 Dosage Unit: ppi .10 .41 .09 .64 .22 .63 .13 .48 .14 .83 .21 .74 -- -.22 .81 .03 . 1 1 .14 .070 8 .58 .241 8 Dosage Unit: pp .23 1.02 .21 .94 .16 .61 .10 .56 . 1 1 .50 .15 .92 .13 1.05 -- .24 .63 .10 .85 .16 .055 9 .79 .212 9 a Unable to calculate due to inaccurate urine volume. 1739 Covance 6329-183 3M T-6295 Appendix 5 Individual Urine Chemistry Data Females Week 27 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER U NA MMOL/L UK MMOL/L NA EXC MMOL K EXC MMOL Group: 3 C93003 C93004 C93011 C93020 C93027 C93030 C93034 C93046 C93047 C93049 MEAN S.D. N Group: 4 C93052 a C93055 C93072 C93080 C93081 C93090 C93094 C93095 C93103 C93104 MEAN S.D. N Dose Level: 2. 0 4 42 13 29 11 54 6 6 10 39 21 18.1 10 20 108 31 86 46 182 82 30 30 86 70 49. 9 10 Dose Level: 5. 0 3 10 54 152 27 80 5 23 3 17 11 44 21 48 38 180 13 56 5 58 18 17.2 10 67 56. 7 10 Dosage Unit: pp' .17 .14 .28 .26 .14 .22 .04 .12 .30 .24 .19 .083 10 .84 .37 .67 .78 .59 .73 .57 .58 .89 .53 .66 .158 10 Dosage Unit: pp -- .30 .84 .26 .76 .15 .70 .12 .67 .21 .84 .52 1.20 .06 .31 .26 1 . 1 2 .10 1.13 .22 .139 9 .84 .280 9 a Unable to calculate due to inaccurate urine volume. 1740 Covance 6329-183 3M T-6295 Appendix 5 Individual Urine Chemistry Data Females Week 27 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER U NA MMOL/L UK MMOL/L NA EXC MMOL K EXC MMOL Group: 5 C93119 C93131 C93133 C93138 C93149 C93153 C93157 C93160 C93162 C93179 MEAN S.D. N Dose Level : 20.0 5 20 36 103 133 319 7 38 28 91 32 71 3 24 20 100 28 71 32 124 32 37.4 10 96 85..7 10 Dosage Unit: pp' .12 .49 .21 .60 .16 .38 .10 .52 .32 1.05 .36 .80 .12 .98 .18 .90 .44 1 . 1 2 .12 .48 .21 .119 10 .73 .270 10 Covance 6329-183 3M T-6295 1741 Appendix 5 Individual Urine Chemistry Data Males Week 53 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER U NA MMOL/L UK MMOL/L NA EXC MMOL K EXC MMOL Group: 1 C92501 C92504 C92507 C92512 C92517 C92523 C92534 C92540 C92562 C92570 MEAN S.D. N Group: 2 C92573 C92587 C92592 C92596 C92604 C92612 C92614 C92624 a C92625 C92629 MEAN S.D. N Dose Level: 0 421007 171 71 37 36 116 59 228 16 130 38 236 45 160 2228 105 80 32 15.5 10 133 65. 6 10 Dose Level: 0. 5 18 174 32 108 46 160 1107 10688 12 107 61 204 37282 245 9 70 23 1108.2 144 10130. 4 Dosage Unit: ppm .63 2.27 .38 1.35 .30 .35 11..1113 ..1318 1..8488 .17 1.09 .40 .27 11..0404 .36 1.30 .34 .141 10 1.30 .390 10 Dosage Unit: ppm .18 1.77 .61 2.05 .44 1.54 .27 1.71 .28 1.90 .19 1.71 .13 .79 -- ..2123 1.64 1.74 .27 .158 9 1.65 .354 9 a Unable to calculate due to inaccurate urine volume. 1742 Covance 6329-183 3M T-6295 Appendix 5 Individual Urine Chemistry Data Males Week 53 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER U NA MMOL/L UK MMOL/L NA EXC MMOL K EXC MMOL Group: 3 C92634 C92635 C92643 C92649 C92653 C92654 C92667 C92669 C92680 C92686 MEAN S.D. N Group: 4 C92694 C92704 C92710 C92711 C92727 C92729 C92739 C92741 C92742 C92748 MEAN S.D. N Dose Level: 2.0 24 19 9 15 11 19 10 44 31 32 21 11.4 10 111 96 78 73 82 56 62 128 144 129 96 30. 6 10 Dose Level: 5.0 5 61 58 335 19 127 29 138 12 99 26 146 32 166 39 143 77 380 24 84 32 21.5 10 168 105. 3 10 Dosage Unit: ppm .21 .97 .21 1.08 .09 .79 .24 1.18 .27 2. 01 .57 1. 68 .26 1.64 .64 1. 86 .32 1.47 .32 1.29 .31 .168 10 1.40 .401 10 Dosage Unit: ppm .14 1.74 .23 1.34 .16 1.08 .26 1.24 .15 1.25 .29 1.61 .26 1.36 .44 1.63 .25 1 . 22 .48 1. 66 .27 .115 10 1.41 .228 10 Covance 6329-183 3M T-6295 1743 Appendix 5 Individual Urine Chemistry Data Males Week 53 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER U NA MMOL/L UK MMOL/L NA EXC MMOL K EXC MMOL Group: 5 C92751 C92754 C92755 C92778 C92790 C92795 C92796 C92817 C92820 MEAN S.D. N Dose Level : 20.0 85 155 51 135 20 135 54 130 48 192 31 1 1 1 23 1 1 1 58 215 41 119 46 20.0 9 145 36.4 9 Dosage Unit: pp' .80 1.46 .56 1.48 .06 .40 .66 1.59 .32 1.29 .54 1.93 .37 1.78 .53 1.98 .58 1.69 .49 .215 9 1.51 .473 9 Covance 6329-183 3M T-6295 1744 Appendix 5 Individual Urine Chemistry Data Females Week 53 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER U NA MMOL/L UK MMOL/L NA EXC MMOL K EXC MMOL Group: 1 C92862 C92876 C92889 C92894 C92899 C92910 C92917 C92922 C92923 C92930 MEAN S.D. N Group: 2 C92931 C92938 C92946 C92952 C92954 C92958 C92964 C92972 C92985 C92990 MEAN S.D. N Dose Level: 0 30 99 51 167 37 91 29 100 74 186 25 144 32 85 53 209 37 129 37 136 40 14.8 10 135 42. 4 10 Dose Level: 0. 5 40 99 28 228 59 119 16 94 20 140 35 182 17 166 22 70 26 152 16 70 28 13.6 10 132 51. 3 10 Dosage Unit: ppm .20 .67 .41 1.34 .39 .96 .25 .85 .58 1.45 .22 1.30 .45 1.19 .47 1. 86 .30 1.03 .35 1.28 .36 .122 10 1.19 .337 10 Dosage Unit: ppm .61 1.51 .08 .68 .34 .68 .15 .89 .09 .66 .23 1.18 .06 .63 .24 .75 .15 .87 .20 .88 .22 .163 10 .87 .278 10 Covance 6329-183 3M T-6295 1745 Appendix 5 Individual Urine Chemistry Data Females Week 53 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER U NA MMOL/L UK MMOL/L NA EXC MMOL K EXC MMOL Group: 3 C92992 C92997 C93012 C93022 C93027 C93029 C93032 C93042 C93046 C93048 MEAN S.D. N Group: 4 C93052 C93054 C93060 C93078 C93081 C93083 C93091 C93095 C93097 C93110 MEAN S.D. N Dose Level : 2.0 14 125 32 304 24 119 22 126 9 56 24 182 39 176 21 133 15 68 26 1 1 1 23 8.8 10 140 69. 9 10 Dose Level: 5.0 59 254 47 261 53 146 18 139 40 94 12 24 31 155 34 283 37 211 39 153 37 14.5 10 172 80.9 10 Dosage Unit: ppm .10 .88 .06 .58 .33 1.64 . 1 1 .63 .18 1.13 .12 .87 .25 1.13 .19 1 . 2 1 .21 .95 .21 .89 .18 .081 10 .99 .307 10 Dosage Unit: ppm T-6295 .19 .84 .13 .73 .30 .83 .04 .35 .33 .77 .52 1.04 .13 .65 .05 .42 .16 .89 .28 1.09 .21 .147 10 .76 .239 10 Covance 6329-183 3M T-6295 1746 Appendix 5 Individual Urine Chemistry Data Females Week 53 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS ANIMAL NUMBER U NA MMOL/L UK MMOL/L NA EXC MMOL K EXC MMOL Group: 5 C93112 C93116 C93117 C93120 C93130 C93138 a C93147 C93154 C93163 C93171 MEAN S.D. N Dose Level : 20.0 27 149 21 133 40 130 14 102 73 120 10 23 60 158 61 300 48 246 64 275 42 22.7 10 164 85.4 10 Dosage Unit: ppm T- .23 1.30 .13 .82 .29 .95 .09 .66 .72 1.18 -.26 .68 .15 .75 .18 .93 .26 1 . 1 0 .26 .186 9 .93 .226 9 a Unable to calculate due to inaccurate urine volume. Covance 6329-183 3M T-6295 1747 COVAH^ THE DEVELOPMENT SERVICES COMPANY Sponsor: 3M St. Paul, Minnesota FINAL REPORT Study Title: 104-Week Dietary Chronic Toxicity and Carcinogenicity Study with Perfluorooctane Sulfonic Acid Potassium Salt (PFOS; T-6295) in Rats Author: Peter J. Thomford, PhD Study Completion Date: January 2, 2002 Testing Facility: Covance Laboratories Inc. 3301 Kinsman Boulevard Madison, Wisconsin 53704-2595 Laboratory Study Identification: Covance 6329-183 Sponsor Study Identification: 3M T-6295 Volume V of IX Page 1748 o f 4068 Covance 6329-183 3M T-6295 VOLUME V OF IX CONTENTS Page APPENDIX 6................................................................................................................ 1750 Individual Anatomic Pathology Data........................................................................1751 1749 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 1750 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1 ANIMAL NUMBER: C92501 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 850.2 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 11:33 PROSECTOR: JOHN S. HALFORD RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT' (%) LF ADRENAL (AD0) RT ADRENAL (AD1) BRAIN (BR) LF KIDNEY (KD0) RT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LF TESTIS (TE0) RT TESTIS (TE1) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) .0462 .0172 2.2353 2.4173 2.4867 25.4814 2.3481 1.1213 1.8183 1.7568 .0346 .0258 .0054 % .0020 % .2629 % .2843 % .2925 % 2.9971 % .2762 % .1319 % .2139 % .2066 % .0041 % .0030 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0207 .0077 1.0000 1.0814 1.1125 11.3995 1.0505 .5016 .8134 .7859 .0155 .0115 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MODERATE SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SLIGHT 1751 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2 ANIMAL NUMBER: C92501 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 850.2 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 11:33 PROSECTOR: JOHN S. HALFORD RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN -ANIMAL OBESE PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-SLIGHT MARROW, FEMUR (FM) : -HYPOCELLULAR, MARROW,-SLIGHT THYMUS (TH) : -HEMORRHAGE,-PRESENT -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MINIMAL THYROID (TY) : -M-CARCINOMA, FOLLICULAR CELL,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 1752 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 3 ANIMAL NUMBER: C92501 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 850.2 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 11:33 PROSECTOR: JOHN S. HALFORD RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 1753 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 4 ANIMAL NUMBER: C92502 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/04/99 STUDY DAY OF DEATH: 533 STUDY WEEK OF DEATH: 77 TERMINAL BODY WEIGHT: 495.7 GRAMS DATE AND TIME OF NECROPSY: 10/04/99 14:45 PROSECTOR: LAURIE J. SCHULLER RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >EYES; RED DISCHARGE; EYE-LEFT; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; ATAXIC; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 9 X 7 X 6 MM, RED, DARK RED, TAN, AND SOFT HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -HYPERPLASIA, BILE DUCT,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -HYALINE DROPLETS, TUBULAR EPITHELIUM,MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATELY SEVERE HEART (HT) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL 1754 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 5 ANIMAL NUMBER: C92502 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/04/99 STUDY DAY OF DEATH: 533 STUDY WEEK OF DEATH: 77 TERMINAL BODY WEIGHT: 495.7 GRAMS DATE AND TIME OF NECROPSY: 10/04/99 14:45 PROSECTOR: LAURIE J. SCHULLER RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY SEMINAL VESICLES (SV) : -LARGE; BILATERAL: EACH IS 25 X 14 X 3 MM GENERAL COMMENT (GC) -BLOOD FILM TAKEN -DISCHARGE-OCULAR; MATERIAL -ANIMAL THIN : LEFT: DARK RED DRY JEJUNUM (JE) : >AUTOLYTIC PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT SEMINAL VESICLES (SV) : >UNREMARKABLE >NOTE:>SEE COAGULATING GLAND CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT COAGULATING GL (CG) : -SECRETORY MATERIAL, INCREASED,-PRESENT 1755 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 6 ANIMAL NUMBER: C92502 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/04/99 STUDY DAY OF DEATH: 533 STUDY WEEK OF DEATH: 77 TERMINAL BODY WEIGHT: 495.7 GRAMS DATE AND TIME OF NECROPSY: 10/04/99 14:45 PROSECTOR: LAURIE J. SCHULLER RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 1756 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 7 ANIMAL NUMBER: C92503 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 419.5 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 11:10 PROSECTOR: JAMES T. CALLEN RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); TAIL-PROXIMAL; WEEK 72; 15 X 10MM; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); TAIL-PROXIMAL; WEEK 105; 15 X 10MM BOTTOM; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY TESTIS (TE) : -SMALL; LEFT: 18 X 9 X 5 MM (RIGHT IS 25 X 11 X 10 MM) THYMUS (TH) : -MASS(ES); INVOLVING ENTIRE ORGAN: SINGLE MASS, 30 X 26 X 15 MM, RED, DARK RED, TAN, AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (MEDIASTINAL) NOT IDENTIFIED HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL -FIBROSIS, PERIBILIARY,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -INFLAMMATION, SUPPURATIVE, PELVIC,SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-MINIMAL ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-SLIGHT -HYPERPLASIA, CORTICAL,-MINIMAL -PIGMENT,-MINIMAL TESTIS (TE) : -ATROPHY/DEGENERATION,-SEVERE >NOTE:>UNILATERAL THYMUS (TH) : >UNREMARKABLE >NOTE:>SEE CAVITY, THORACIC FOR DIAGNOSIS 1757 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 8 ANIMAL NUMBER: C92503 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 419.5 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 11:10 PROSECTOR: JAMES T. CALLEN RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -RED FOCUS(I)/AREA(S); LEFT ASPECT: SINGLE DARK RED FOCUS, 1 MM IN DIAMETER STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: MULTIPLE DARK BROWN AREAS, UP TO 2 X 1 MM PROSTATE (PR) : -SMALL; 10 MM IN DIAMETER SEMINAL VESICLES (SV) : -SMALL; BILATERAL: EACH IS 19 X 12 X 3 MM LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL HEART (HT) : -CARDIOMYOPATHY,-SLIGHT THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : >UNREMARKABLE PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT SEMINAL VESICLES (SV) : -SECRETION, INCREASED,-PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT COAGULATING GL (CG) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE TAIL (TI) : -CYST, EPIDERMAL INCLUSION,-PRESENT 1758 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 9 ANIMAL NUMBER: C92503 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 419.5 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 11:10 PROSECTOR: JAMES T. CALLEN RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY TAIL (TI) : -MASS(ES); NEAR BASE OF TAIL: TWO MASSES, EACH IS 12 X 7 X 5 MM, EACH IS TAN AND FIRM; SAME ON CUT SECTIONS; COLLECTED; REGIONAL LN (SEE LUMBAR) LN, LUMBAR/ILIAC (LM) : -DIFFUSELY RED; LEFT: DARK RED (CAP 23) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -ANIMAL THIN LN, LUMBAR/ILIAC (LM) : -HEMORRHAGE,-PRESENT -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL CAVITY, THORACIC (TA) : -B-AORTIC BODY TUMOR,-NEOPLASM PRESENT 1759 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 10 ANIMAL NUMBER: C92503 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 419.5 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 11:10 PROSECTOR: JAMES T. CALLEN RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 1760 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 11 ANIMAL NUMBER: C92504 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 650.0 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 9:35 PROSECTOR: KEVIN BILLINGS RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT' (%) LF ADRENAL (AD0) RT ADRENAL (AD1) BRAIN (BR) LF KIDNEY (KD0) RT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LF TESTIS (TE0) RT TESTIS (TE1) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) .0300 .0306 2.0966 2.2133 2.1217 16.8986 2.0480 .8682 2.1700 2.2147 .0181 .0171 .0046 % .0047 % .3226 % .3405 % .3264 % 2.5998 % .3151 % .1336 % .3338 % .3407 % .0028 % .0026 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0143 .0146 1.0000 1.0557 1.0120 8.0600 .9768 .4141 1.0350 1.0563 .0086 .0082 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED LIVER (LI) : -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, PERIPORTAL,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, PELVIC,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MINIMAL 1761 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 12 ANIMAL NUMBER: C92504 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 650.0 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 9:35 PROSECTOR: KEVIN BILLINGS RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN -MALOCCLUSION; BILATERAL: UPPER INCISORS SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MINIMAL ADRENAL, CORTEX (AC) : -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL THYMUS (TH) : >SECTION EXAMINED; TISSUE NOT PRESENT PITUITARY (PI) : -HYPERPLASIA,-MINIMAL URINARY BLADDER (UB) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL EPIDIDYMIDES (EP) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 1762 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 13 ANIMAL NUMBER: C92504 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 650.0 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 9:35 PROSECTOR: KEVIN BILLINGS RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), SEMINAL VESICLES (SV), HEMATO NEOPLASIA (HN) 1763 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 14 ANIMAL NUMBER: C92505 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 644.5 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 13:38 PROSECTOR: JARED FISHEL RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-SLIGHT LIVER-OIL RED O (LI0) : -VACUOLES, OIL RED O-POSITIVE, HEPATOCELLULAR,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SLIGHT PANCREAS (PA) : -INFLAMMATION, VASCULAR,-MODERATE ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-MINIMAL -PIGMENT,-SLIGHT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),SLIGHT LUNG (LU) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL 1764 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 15 ANIMAL NUMBER: C92505 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 644.5 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 13:38 PROSECTOR: JARED FISHEL RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY THYROID (TY) : -LARGE; RIGHT: 9 X 7 X 6 MM (LEFT IS 7 X 3 X 3 MM) -DIFFUSELY DARK; RIGHT: DARK BROWN STOMACH, GL (ST) : -THICKENED WALL; DIFFUSE JEJUNUM (JE) : -THICKENED WALL; ILEUM (IL) : -THICKENED WALL; DIFFUSE DIFFUSE HEART (HT) : -CARDIOMYOPATHY,-MINIMAL THYROID (TY) : -M-CARCINOMA, FOLLICULAR CELL,-NEOPLASM PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : -INFLAMMATION, CHRONIC-ACTIVE,MODERATELY SEVERE -HYPERPLASIA, EPITHELIAL, GLANDULAR,MODERATELY SEVERE DUODENUM (DU) : -INFLAMMATION, CHRONIC-ACTIVE,MODERATELY SEVERE JEJUNUM (JE) : >UNREMARKABLE ILEUM (IL) : -INFLAMMATION, CHRONIC-ACTIVE,MODERATELY SEVERE CECUM (CE) : -INFLAMMATION, CHRONIC-ACTIVE,MODERATELY SEVERE COLON (CO) : -INFLAMMATION, CHRONIC-ACTIVE,-SEVERE >NOTE:>EOSINOPHILS PREDOMINATE RECTUM (RE) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE 1765 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 16 ANIMAL NUMBER: C92505 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 644.5 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 13:38 PROSECTOR: JARED FISHEL RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL EPIDIDYMIDES (EP) : -INFILTRATE, LYMPHOHISTIOCYTIC,-SLIGHT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 1766 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 17 ANIMAL NUMBER: C92505 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 644.5 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 13:38 PROSECTOR: JARED FISHEL RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB STOMACH, NONGL (SU), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), HEMATO NEOPLASIA (HN) (SG), 1767 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 18 ANIMAL NUMBER: C92506 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 330.0 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 10:54 PROSECTOR: JODI THIEREN RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN STATUS LF ADRENAL (AD0) RT ADRENAL (AD1) BRAIN (BR) LF KIDNEY (KD0) RT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LF TESTIS (TE0) RT TESTIS (TE1) LF THYROID/PARA RT THYROID/PARA (TT0) (TT1) ------------------------------------ 10.8663 ------------------------------------------- ------------------------------------ 3.2924 % ------------------------------------------- ------------------------------------------------------------------------------------- NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN WEIGHT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS 1768 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 19 ANIMAL NUMBER: C92506 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 330.0 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 10:54 PROSECTOR: JODI THIEREN RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) 1769 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 20 ANIMAL NUMBER: C92507 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 636.5 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 10:49 PROSECTOR: TODD ACKER RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT' (%) LF ADRENAL (AD0) RT ADRENAL (AD1) BRAIN (BR) LF KIDNEY (KD0) RT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LF TESTIS (TE0) RT TESTIS (TE1) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) .0224 .0247 2.3724 2.1481 2.1112 20.1131 2.1448 .9408 1.8775 1.7754 .0301 .0303 .0035 % .0039 % .3727 % .3375 % .3317 % 3.1600 % .3370 % .1478 % .2950 % .2789 % .0047 % .0048 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0094 .0104 1.0000 .9055 .8899 8.4780 .9041 .3966 .7914 .7484 .0127 .0128 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -VACUOLATION, HEPATOCELLULAR, MIDZONAL / CENTRILOBULAR,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-SLIGHT -HYPERPLASIA, BILE DUCT,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT 1770 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 21 ANIMAL NUMBER: C92507 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 636.5 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 10:49 PROSECTOR: TODD ACKER RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN ADRENAL, CORTEX (AC) : -HYPERTROPHY, CORTICAL CELL,-MINIMAL MARROW, FEMUR (FM) : -HYPOCELLULAR, MARROW,-MODERATE HEART (HT) : -CARDIOMYOPATHY,-MINIMAL PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 1771 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 22 ANIMAL NUMBER: C92507 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 636.5 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 10:49 PROSECTOR: TODD ACKER RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 1772 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 23 ANIMAL NUMBER: C92508 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 10/08/99 STUDY DAY OF DEATH: 537 STUDY WEEK OF DEATH: 77 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 10/08/99 8:23 PROSECTOR: NOT AVAILABLE RECORDER: NOT AVAILABLE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: NOT AVAILABLE WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; ROUGH HAIR COAT; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 11 X 8 X 6 MM, RED, DARK RED, TAN AND SOFT HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, PELVIC,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL JEJUNUM (JE) : >AUTOLYTIC ILEUM (IL) : >AUTOLYTIC CECUM (CE) : >AUTOLYTIC 1773 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 24 ANIMAL NUMBER: C92508 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 10/08/99 STUDY DAY OF DEATH: 537 STUDY WEEK OF DEATH: 77 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 10/08/99 8:23 PROSECTOR: NOT AVAILABLE RECORDER: NOT AVAILABLE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: NOT AVAILABLE WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY URINARY BLADDER (UB) : -CALCULUS(I); WITHIN LUMEN: SINGLE WHITE, FIRM CALCULI, 6 MM IN DIAMETER GENERAL COMMENT (GC) : -DISCHARGE-OCULAR; BILATERAL: DARK RED, DRY MATERIAL -DISCHARGE-NASAL; RED, DRY MATERIAL URINARY BLADDER (UB) : >AUTOLYTIC PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 1774 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 25 ANIMAL NUMBER: C92508 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 10/08/99 STUDY DAY OF DEATH: 537 STUDY WEEK OF DEATH: 77 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 10/08/99 8:23 PROSECTOR: NOT AVAILABLE RECORDER: NOT AVAILABLE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: NOT AVAILABLE WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUm /s KULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), COLON (CO), RECTUM (RE), SKIN (SK), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 1775 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 26 ANIMAL NUMBER: C92509 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 491.0 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 11:18 PROSECTOR: DONIA MEYER RECORDER: KIMBERLY DURLAND POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT' (%) LF ADRENAL (AD0) RT ADRENAL (AD1) BRAIN (BR) LF KIDNEY (KD0) RT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LF TESTIS (TE0) RT TESTIS (TE1) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) .0322 .0308 2.3068 1.7786 1.8602 14.5106 1.7988 .8923 1.8453 1.8482 .0131 .0128 .0066 % .0063 % .4698 % .3622 % .3789 % 2.9553 % .3664 % .1817 % .3758 % .3764 % .0027 % .0026 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0140 .0134 1.0000 .7710 .8064 6.2904 .7798 .3868 .7999 .8012 .0057 .0055 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL KIDNEY (KD) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MINIMAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 1776 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 27 ANIMAL NUMBER: C92509 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 491.0 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 11:18 PROSECTOR: DONIA MEYER RECORDER: KIMBERLY DURLAND POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE) 1777 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 28 ANIMAL NUMBER: C92510 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 765.1 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 13:22 PROSECTOR: JAMES T. CALLEN RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-SLIGHT -DEGENERATION, CYSTIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL -FIBROSIS, PERIBILIARY,-MINIMAL LIVER-OIL RED O (LI0) : -VACUOLES, OIL RED O-POSITIVE, HEPATOCELLULAR,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SLIGHT ADRENAL, CORTEX (AC) : -HYPERPLASIA, CORTICAL,-MINIMAL -PIGMENT,-MINIMAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),SLIGHT LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL 1778 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 29 ANIMAL NUMBER: C92510 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 765.1 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 13:22 PROSECTOR: JAMES T. CALLEN RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN HEART (HT) : -CARDIOMYOPATHY,-SLIGHT THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 1779 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 30 ANIMAL NUMBER: C92510 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 765.1 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 13:22 PROSECTOR: JAMES T. CALLEN RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 1780 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 31 ANIMAL NUMBER: C92511 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 506.8 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 12:55 PROSECTOR: JODI THIEREN RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT' (%) LF ADRENAL (AD0) RT ADRENAL (AD1) BRAIN (BR) LF KIDNEY (KD0) RT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LF TESTIS (TE0) RT TESTIS (TE1) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) .0348 .0213 2.0610 1.9719 1.9652 16.1948 2.1768 .7816 1.9467 1.9871 .0109 .0139 .0069 % .0042 % .4067 % .3891 % .3878 % 3.1955 % .4295 % .1542 % .3841 % .3921 % .0022 % .0027 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0169 .0103 1.0000 .9568 .9535 7.8577 1.0562 .3792 .9445 .9641 .0053 .0067 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED THYMUS (TH) : -RED FOCUS(I)/AREA(S); DIFFUSE: MULTIPLE RED PINPOINT FOCI GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -NECROSIS, INDIVIDUAL HEPATOCYTE,MINIMAL KIDNEY (KD) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL THYMUS (TH) : >NOT REQUIRED TO BE EXAMINED FOR ANIMAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 1781 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 32 ANIMAL NUMBER: C92511 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 506.8 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 12:55 PROSECTOR: JODI THIEREN RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAr /i LIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE) 1782 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 33 ANIMAL NUMBER: C92512 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 654.1 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 8:48 PROSECTOR: TODD ACKER RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT' (%) LF ADRENAL (AD0) RT ADRENAL (AD1) BRAIN (BR) LF KIDNEY (KD0) RT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LF TESTIS (TE0) RT TESTIS (TE1) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) .0380 .0269 2.4142 2.4170 2.3030 19.0310 2.0416 .8649 2.4547 1.8193 .0165 .0216 .0058 % .0041 % .3691 % .3695 % .3521 % 2.9095 % .3121 % .1322 % .3753 % .2781 % .0025 % .0033 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0157 .0111 1.0000 1.0012 .9539 7.8829 .8457 .3583 1.0168 .7536 .0068 .0089 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MINIMAL 1783 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 34 ANIMAL NUMBER: C92512 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 654.1 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 8:48 PROSECTOR: TODD ACKER RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL TESTIS (TE) : -ATROPHY/DEGENERATION,-SLIGHT MARROW, FEMUR (FM) : -HYPOCELLULAR, MARROW,-SLIGHT LUNG (LU) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 1784 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 35 ANIMAL NUMBER: C92512 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 654.1 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 8:48 PROSECTOR: TODD ACKER RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) (CE), 1785 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 36 ANIMAL NUMBER: C92513 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 751.7 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 13:27 PROSECTOR: GREGORY CODDINGTON RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-SLIGHT -HYPERPLASIA, BILE DUCT,-MINIMAL -VACUOLATION, HEPATOCELLULAR,-MINIMAL LIVER-OIL RED O (LI0) : -VACUOLES, OIL RED O-POSITIVE, HEPATOCELLULAR,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MINIMAL ADRENAL, CORTEX (AC) : -PIGMENT,-SLIGHT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MINIMAL LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL HEART (HT) : -CARDIOMYOPATHY,-SLIGHT 1786 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 37 ANIMAL NUMBER: C92513 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 751.7 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 13:27 PROSECTOR: GREGORY CODDINGTON RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN THYROID (TY) : -DILATATION / CYST, FOLLICULAR,-PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT -HYPERPLASIA,-SLIGHT HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL STOMACH, GL (ST) : -HYPERPLASIA, EPITHELIAL, GLANDULAR,MODERATE PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 1787 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 38 ANIMAL NUMBER: C92513 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 751.7 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 13:27 PROSECTOR: GREGORY CODDINGTON RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 1788 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 39 ANIMAL NUMBER: C92514 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 1041.3 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 8:40 PROSECTOR: JAMES T. CALLEN RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY HISTOPATHOLOGY EYE (EY) : -DYSTROPHY, CORNEAL IN EARLY STAGES, CONSISTS PRIMARILY OF FOCAL MINERALIZATION,-MINIMAL LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL LIVER-OIL RED O (LI0) : -VACUOLES, OIL RED O-POSITIVE, HEPATOCELLULAR,-MODERATE SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MINIMAL PANCREAS (PA) : -INFILTRATION, FAT, INTERSTITIAL,SLIGHT ADRENAL, MEDULLA (MA) : -UNILATERALLY EXAMINED,-PRESENT LUNG (LU) : -MINERALIZATION, VASCULAR,-SLIGHT 1789 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 40 ANIMAL NUMBER: C92514 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 1041.3 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 8:40 PROSECTOR: JAMES T. CALLEN RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY STOMACH, GL (ST) : -THICKENED WALL; ALL REGIONS DIFFUSE GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -ANIMAL OBESE LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL HEART (HT) : -CARDIOMYOPATHY,-SLIGHT PARATHYROID (PT) : -B-ADENOMA,-NEOPLASM PRESENT PITUITARY (PI) : -HYPERPLASIA,-MODERATE STOMACH, GL (ST) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT -HYPERPLASIA, EPITHELIAL, GLANDULAR,SLIGHT COLON (CO) : -PARASITISM,-PRESENT RECTUM (RE) : -PARASITISM,-PRESENT EPIDIDYMIDES (EP) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 1790 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 41 ANIMAL NUMBER: C92514 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 1041.3 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 8:40 PROSECTOR: JAMES T. CALLEN RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: BRAIN (BR), KIDNEY (KD), ADRENAL, CORTEX (AC), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), MUSCLE, SKELETAL NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), HEMATO NEOPLASIA (HN) (SM), 1791 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 42 ANIMAL NUMBER: C92515 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/12/00 STUDY DAY OF DEATH: 693 STUDY WEEK OF DEATH: 99 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/12/00 13:12 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY HISTOPATHOLOGY EYE (EY) : -DYSTROPHY, CORNEAL IN EARLY STAGES, CONSISTS PRIMARILY OF FOCAL MINERALIZATION,-SLIGHT LIVER (LI) : -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, PELVIC,-MINIMAL PANCREAS (PA) : -INFILTRATION, FAT, INTERSTITIAL,MINIMAL ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-MINIMAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATELY SEVERE THYROID (TY) : -B-ADENOMA, FOLLICULAR CELL,-NEOPLASM PRESENT PITUITARY (PI) : -HYPERPLASIA,-SLIGHT 1792 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 43 ANIMAL NUMBER: C92515 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/12/00 STUDY DAY OF DEATH: 693 STUDY WEEK OF DEATH: 99 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/12/00 13:12 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS JEJUNUM (JE) : >AUTOLYTIC ILEUM (IL) : >AUTOLYTIC CECUM (CE) : >AUTOLYTIC PROSTATE (PR) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL EPIDIDYMIDES (EP) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL CAUSE OF DEATH (CD) : -UNDETERMINED MICROSCOPICALLY,-PRESENT 1793 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 44 ANIMAL NUMBER: C92515 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/12/00 STUDY DAY OF DEATH: 693 STUDY WEEK OF DEATH: 99 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/12/00 13:12 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: BRAIN (BR), SPLEEN (SP), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), HEMATO NEOPLASIA (HN) 1794 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 45 ANIMAL NUMBER: C92516 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/11/99 STUDY DAY OF DEATH: 326 STUDY WEEK OF DEATH: 47 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/11/99 7:46 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY HISTOPATHOLOGY LIVER (LI) : -VACUOLATION, HEPATOCELLULAR, MIDZONAL / CENTRILOBULAR,-MINIMAL -CONGESTION,-PRESENT KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MINIMAL ADRENAL, CORTEX (AC) : -CONGESTION,-PRESENT LN, MESENTERIC (MS) : >TISSUE MISSING MARROW, FEMUR (FM) : -HYPOCELLULAR, MARROW,-MODERATE THYMUS (TH) : -HEMORRHAGE,-PRESENT -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATE LUNG (LU) : -CONGESTION,-PRESENT HEART (HT) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FIBROSIS,-MINIMAL 1795 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 46 ANIMAL NUMBER: C92516 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/11/99 STUDY DAY OF DEATH: 326 STUDY WEEK OF DEATH: 47 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/11/99 7:46 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS DUODENUM (DU) : >AUTOLYTIC JEJUNUM (JE) : >AUTOLYTIC ILEUM (IL) : >AUTOLYTIC CAUSE OF DEATH (CD) : -UNDETERMINED MICROSCOPICALLY,-PRESENT 1796 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 47 ANIMAL NUMBER: C92516 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/11/99 STUDY DAY OF DEATH: 326 STUDY WEEK OF DEATH: 47 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/11/99 7:46 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, MEDULLA (MA), TESTIS (TE), BONE, FEMUR (FE), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 1797 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 48 ANIMAL NUMBER: C92517 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 913.7 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 11:04 PROSECTOR: JOHN S. HALFORD RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT' (%) LF ADRENAL (AD0) RT ADRENAL (AD1) BRAIN (BR) LF KIDNEY (KD0) RT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LF TESTIS (TE0) RT TESTIS (TE1) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) .0325 .0365 2.3607 2.6614 2.6775 24.8245 2.5656 1.1036 1.8990 1.9440 .0289 .0308 .0036 % .0040 % .2584 % .2913 % .2930 % 2.7169 % .2808 % .1208 % .2078 % .2128 % .0032 % .0034 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0138 .0155 1.0000 1.1274 1.1342 10.5157 1.0868 .4675 .8044 .8235 .0122 .0130 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SLIGHT 1798 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 49 ANIMAL NUMBER: C92517 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 913.7 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 11:04 PROSECTOR: JOHN S. HALFORD RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY LUNG (LU) : -DARK FOCUS(I)/AREA(S); LEFT LOBE: SINGLE BLACK FOCUS, 2 MM IN DIAMETER GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN -ANIMAL OBESE PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL -HYPERPLASIA, ISLET CELLS,-MINIMAL ADRENAL, CORTEX (AC) : -HYPERTROPHY, CORTICAL CELL,-MODERATE THYMUS (TH) : -HEMORRHAGE,-PRESENT -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MINIMAL LUNG (LU) : -CONGESTION,-PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 1799 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 50 ANIMAL NUMBER: C92517 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 913.7 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 11:04 PROSECTOR: JOHN S. HALFORD RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAr /i LIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES HEMATO NEOPLASIA (HN) (SM), (CE), (EP), 1800 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 51 ANIMAL NUMBER: C92518 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 841.2 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 13:38 PROSECTOR: DAVID SCHUETTE RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -RED FOCUS(I)/AREA(S); DIAPHRAGMATIC SURFACE OF RIGHT MEDIAN LOBE: SINGLE DARK RED AREA, 4 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL LIVER-OIL RED O (LI0) : -VACUOLES, OIL RED O-POSITIVE, HEPATOCELLULAR,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MODERATE SPLEEN (SP) : -PIGMENT, INCREASED,-MINIMAL -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE PANCREAS (PA) : -HYPERTROPHY / BASOPHILIA, ACINAR CELL,MINIMAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT -PIGMENT,-MINIMAL THYMUS (TH) : >SECTION EXAMINED; TISSUE NOT PRESENT 1801 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 52 ANIMAL NUMBER: C92518 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 841.2 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 13:38 PROSECTOR: DAVID SCHUETTE RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -RED FOCUS(I)/AREA(S); RIGHT MID ASPECT: SINGLE DARK RED AREA, 2 MM IN DIAMETER GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -ANIMAL OBESE LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL HEART (HT) : -CARDIOMYOPATHY,-MINIMAL THYROID (TY) : -DILATATION / CYST, FOLLICULAR,-PRESENT PARATHYROID (PT) : -HYPERPLASIA,-SLIGHT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 1802 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 53 ANIMAL NUMBER: C92518 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 841.2 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 13:38 PROSECTOR: DAVID SCHUETTE RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 1803 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 54 ANIMAL NUMBER: C92519 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 10/28/99 STUDY DAY OF DEATH: 557 STUDY WEEK OF DEATH: 80 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 10/28/99 10:37 PROSECTOR: JARED FISHEL RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) ADRENAL, CORTEX (AC) : -LARGE; RIGHT: 6 X 4 X 3 MM (LEFT IS 3 X 2 X 2 MM) HISTOPATHOLOGY BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SLIGHT SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL ADRENAL, CORTEX (AC) : -UNILATERALLY EXAMINED,-PRESENT ADRENAL, MEDULLA (MA) : -UNILATERALLY EXAMINED,-PRESENT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATE LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL 1804 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 55 ANIMAL NUMBER: C92519 SEX: :MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 10/28/99 STUDY DAY OF DEATH: 557 STUDY WEEK OF DEATH: 80 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 10/28/99 10:37 PROSECTOR: JARED FISHEL RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 9 X 6 X 6 MM, DARK RED, GREY, AND SOFT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 1805 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 56 ANIMAL NUMBER: C92519 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 10/28/99 STUDY DAY OF DEATH: 557 STUDY WEEK OF DEATH: 80 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 10/28/99 10:37 PROSECTOR: JARED FISHEL RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUm /s KULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG), GENERAL COMMENT (GC) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 1806 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 57 ANIMAL NUMBER: C92520 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/18/99 STUDY DAY OF DEATH: 578 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/18/99 13:45 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; LIMITED USE; LIMB-HINDRIGHT; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; SWOLLEN; DIGIT(S)-HINDRIGHT; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY KIDNEY (KD) : -LARGE PELVIS(ES); BILATERAL -LARGE; BILATERAL: EACH ARE 33 X 23 X 10 MM; BOTH ARE SOFT SPLEEN (SP) : -ADHESION(S); CRANIAL END ADHERED TO DIAPHRAGM: MULTIPLE GRAY, FIBROUS ADHESIONS; COLLECTED INTACT PANCREAS (PA) : -LARGE VESSELS; ALONG DUODENUM: UP TO 5 MM IN DIAMETER; COLLECTED INTACT WITH DUODENUM HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL KIDNEY (KD) : -DILATATION, PELVIC,-MODERATE -MINERALIZATION, TUBULAR,-MINIMAL -ECTASIA, TUBULAR,-SEVERE -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE -FIBROSIS, CORTICAL,-SLIGHT SPLEEN (SP) : -FIBROSIS, CAPSULAR,-PRESENT PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL -INFLAMMATION, VASCULAR,-MODERATE -THROMBUS,-PRESENT ADRENAL, CORTEX (AC) : -HYPERTROPHY, CORTICAL CELL,-MINIMAL LN, MESENTERIC (MS) : -MINERALIZATION, VASCULAR,-MODERATE 1807 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 58 ANIMAL NUMBER: C92520 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/18/99 STUDY DAY OF DEATH: 578 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/18/99 13:45 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY THYROID (TY) : -LARGE; RIGHT: 6 X 4 X 3 MM (LEFT IS 5 X 3 X 3 MM) STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: MULTIPLE DARK BROWN PINPOINT FOCI URINARY BLADDER (UB) : -DIFFUSELY RED; DARK RED MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,MODERATELY SEVERE MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,-SEVERE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),SEVERE HEART (HT) : -CARDIOMYOPATHY,-SLIGHT -MINERALIZATION, VASCULAR,-SLIGHT -MINERALIZATION,-MINIMAL THYROID (TY) : -B-ADENOMA, FOLLICULAR CELL,-NEOPLASM PRESENT PARATHYROID (PT) : -HYPERPLASIA,-MODERATE STOMACH, GL (ST) : >UNREMARKABLE DUODENUM (DU) : -INFLAMMATION, VASCULAR,-MODERATE CECUM (CE) : >AUTOLYTIC URINARY BLADDER (UB) : -INFLAMMATION, CHRONIC-ACTIVE,-SEVERE PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SEVERE 1808 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 59 ANIMAL NUMBER: C92520 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/18/99 STUDY DAY OF DEATH: 578 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/18/99 13:45 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PROSTATE (PR) : -NODULAR; PROXIMAL, SURROUNDING URETHRA: GREEN AND NODULAR GENERAL COMMENT (GC) : -URINARY BLADDER-LARGE LUMEN; DISTENDED TO 35 X 25 X 15 MM WITH A DARK GREEN FLUID SEMINAL VESICLES (SV) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE CAUSE OF DEATH (CD) : -INFLAMMATION / INFECTION,-PRESENT 1809 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 60 ANIMAL NUMBER: C92520 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/18/99 STUDY DAY OF DEATH: 578 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/18/99 13:45 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAr /i LIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), ADRENAL, MEDULLA (MA), TESTIS (TE), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), JEJUNUM (JE), ILEUM (IL), COLON (CO), RECTUM (RE), SKIN (SK), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) (SC), 1810 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 61 ANIMAL NUMBER: C92521 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 467.7 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 10:33 PROSECTOR: TROY ELLENBECKER RECORDER: KIMBERLY DURLAND POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT' (%) LF ADRENAL (AD0) RT ADRENAL (AD1) BRAIN (BR) LF KIDNEY (KD0) RT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LF TESTIS (TE0) RT TESTIS (TE1) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) .0329 .0340 2.2509 2.0805 2.0278 16.3067 1.8853 .7612 1.9720 1.8773 .0130 .0164 .0070 % .0073 % .4813 % .4448 % .4336 % 3.4866 % .4031 % .1628 % .4216 % .4014 % .0028 % .0035 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0146 .0151 1.0000 .9243 .9009 7.2445 .8376 .3382 .8761 .8340 .0058 .0073 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-SLIGHT KIDNEY (KD) : -REGENERATION, TUBULAR EPITHELIUM,MINIMAL TESTIS (TE) : -ATROPHY/DEGENERATION,-MINIMAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 1811 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 62 ANIMAL NUMBER: C92521 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 467.7 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 10:33 PROSECTOR: TROY ELLENBECKER RECORDER: KIMBERLY DURLAND POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS) 1812 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 63 ANIMAL NUMBER: C92522 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/29/00 STUDY DAY OF DEATH: 681 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: 639.8 GRAMS DATE AND TIME OF NECROPSY: 02/29/00 14:29 PROSECTOR: DENNIS HOFFMAN RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; SORE/SCAB; PAWS-HIND; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; VENTRAL ABDOMINAL; VERIFIED AT NECROPSY: OBSERVED >EYES; RED DISCHARGE; EYES; VERIFIED AT NECROPSY: OBSERVED >EYES; SQUINTED; PERI-ORBITAL; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; RECUMBENT; LATERAL; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -DARK FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE DARK RED AREAS, UP TO 7 X 5 MM, COLLECTED ON ROUTINE SECTIONS HISTOPATHOLOGY BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-SLIGHT -HYPERPLASIA, BILE DUCT,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SLIGHT -MINERALIZATION, PELVIC,-MINIMAL PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-MINIMAL -PIGMENT,-SLIGHT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATELY SEVERE THYROID (TY) : -HYPERPLASIA, C-CELL,-SLIGHT 1813 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 64 ANIMAL NUMBER: C92522 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/29/00 STUDY DAY OF DEATH: 681 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: 639.8 GRAMS DATE AND TIME OF NECROPSY: 02/29/00 14:29 PROSECTOR: DENNIS HOFFMAN RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 10 X 8 X 4 MM, DARK RED, BROWN, AND FIRM SKIN (SK) : -CRUSTED AREA(S); VENTRAL ABDOMEN, OVERLYING RIGHT PREPUTIAL GLAND: SINGLE BROWN CRUSTED AREA, 2 X 3 MM (CAP 22) FOOT/FOOTPAD (FP) : -ABRASION(S)/ULCERATION(S); BILATERAL, HIND FEET: SINGLE DRY, RED, AND TAN AREA ON EACH, LEFT IS 11 X 7 MM, RIGHT IS 7 MM IN DIAMETER; COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DARK RED DRY MATERIAL PARATHYROID (PT) : -HYPERPLASIA,-SLIGHT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT SKIN (SK) : -ACANTHOSIS/HYPERKERATOSIS,-SLIGHT -INFLAMMATION, CHRONIC-ACTIVE,MODERATELY SEVERE PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE CAUSE OF DEATH (CD) : -INFLAMMATION / INFECTION,-PRESENT FOOT/FOOTPAD (FP) : -INFLAMMATION, CHRONIC-ACTIVE,-SEVERE -ULCERATION,-MODERATE 1814 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 65 ANIMAL NUMBER: C92522 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/29/00 STUDY DAY OF DEATH: 681 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: 639.8 GRAMS DATE AND TIME OF NECROPSY: 02/29/00 14:29 PROSECTOR: DENNIS HOFFMAN RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), HARDERIAN GLAND (HG), SALIV GL, STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) MANDIB (SG), 1815 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 66 ANIMAL NUMBER: C92523 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 771.0 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 11:24 PROSECTOR: TODD ACKER RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT' (%) LF ADRENAL (AD0) RT ADRENAL (AD1) BRAIN (BR) LF KIDNEY (KD0) RT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LF TESTIS (TE0) RT TESTIS (TE1) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) .0280 .0276 2.5515 2.2588 2.4189 19.4596 1.8566 1.0752 1.8292 1.7216 .0214 .0273 .0036 % .0036 % .3309 % .2930 % .3137 % 2.5239 % .2408 % .1395 % .2373 % .2233 % .0028 % .0035 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0110 .0108 1.0000 .8853 .9480 7.6267 .7277 .4214 .7169 .6747 .0084 .0107 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -VACUOLATION, HEPATOCELLULAR,-MINIMAL KIDNEY (KD) : -REGENERATION, TUBULAR EPITHELIUM,MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT PANCREAS (PA) : -HYPERPLASIA, ISLET CELLS,-MINIMAL 1816 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 67 ANIMAL NUMBER: C92523 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 771.0 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 11:24 PROSECTOR: TODD ACKER RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN ADRENAL, CORTEX (AC) : -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL HEART (HT) : -CARDIOMYOPATHY,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 1817 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 68 ANIMAL NUMBER: C92523 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 771.0 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 11:24 PROSECTOR: TODD ACKER RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) (JE), 1818 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 69 ANIMAL NUMBER: C92524 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 366.6 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 11:59 PROSECTOR: LYNN HORNER RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN STATUS LF ADRENAL (AD0) RT ADRENAL (AD1) BRAIN (BR) LF KIDNEY (KD0) RT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LF TESTIS (TE0) RT TESTIS (TE1) LF THYROID/PARA RT THYROID/PARA (TT0) (TT1) ------------------------------------ 14.3739 ------------------------------------------- ------------------------------------ 3.9212 % ------------------------------------------- ------------------------------------------------------------------------------------- NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN WEIGHT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS 1819 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 70 ANIMAL NUMBER: C92524 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 366.6 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 11:59 PROSECTOR: LYNN HORNER RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) 1820 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 71 ANIMAL NUMBER: C92525 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/30/99 STUDY DAY OF DEATH: 498 STUDY WEEK OF DEATH: 72 TERMINAL BODY WEIGHT: 474.0 GRAMS DATE AND TIME OF NECROPSY: 08/30/99 13:54 PROSECTOR: WENDY CHERRY RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); VENTRAL-ABDOMINAL; WEEK 61; 10 X 10MM; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: NOT OBSERVED >SKIN & PELAGE; ROUGH HAIR COAT; VERIFIED AT NECROPSY: NOT APPLICABLE >EXCRETION; NO FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; MODERATE; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 17 X 12 X 10 MM, TAN, BROWN, DARK RED AND SOFT HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, PELVIC,-MINIMAL -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-SLIGHT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),SLIGHT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT SKIN (SK) : -CYST, EPIDERMAL INCLUSION,-PRESENT 1821 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 72 ANIMAL NUMBER: C92525 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/30/99 STUDY DAY OF DEATH: 498 STUDY WEEK OF DEATH: 72 TERMINAL BODY WEIGHT: 474.0 GRAMS DATE AND TIME OF NECROPSY: 08/30/99 13:54 PROSECTOR: WENDY CHERRY RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PROSTATE (PR) : -SMALL; 1 0 X 1 0 X 6 MM SEMINAL VESICLES (SV) : -SMALL; BILATERAL: EACH IS 15 X 8 X 2 MM MAMMARY, MALE (MM) : -MASS(ES); MID ABDOMINAL REGION: SINGLE MASS, 17 X 12 X 10 MM, TAN, BROWN AND FIRM; CUT SURFACE CONTAINS A TAN AND CASEOUS SEMISOLID; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,MODERATELY SEVERE SEMINAL VESICLES (SV) : -SECRETION, DECREASED,-PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT MAMMARY, MALE (MM) : >NOT REQUIRED TO BE EXAMINED FOR ANIMAL >NOTE:>SEE SKIN FOR HISTOLOGIC DIAGNOSIS 1822 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 73 ANIMAL NUMBER: C92525 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/30/99 STUDY DAY OF DEATH: 498 STUDY WEEK OF DEATH: 72 TERMINAL BODY WEIGHT: 474.0 GRAMS DATE AND TIME OF NECROPSY: 08/30/99 13:54 PROSECTOR: WENDY CHERRY RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (JE), (HN) 1823 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 74 ANIMAL NUMBER: C92526 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 325.6 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 11:33 PROSECTOR: JODI THIEREN RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN STATUS LF ADRENAL (AD0) RT ADRENAL (AD1) BRAIN (BR) LF KIDNEY (KD0) RT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LF TESTIS (TE0) RT TESTIS (TE1) LF THYROID/PARA RT THYROID/PARA (TT0) (TT1) ------------------------------------ 12.0558 ------------------------------------------- ------------------------------------ 3.7028 % ------------------------------------------- ------------------------------------------------------------------------------------- NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN WEIGHT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS 1824 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 75 ANIMAL NUMBER: C92526 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 325.6 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 11:33 PROSECTOR: JODI THIEREN RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) 1825 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 76 ANIMAL NUMBER: C92527 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/24/99 STUDY DAY OF DEATH: 584 STUDY WEEK OF DEATH: 84 TERMINAL BODY WEIGHT: 561.4 GRAMS DATE AND TIME OF NECROPSY: 11/24/99 8:24 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; HEAD TILT; RIGHT; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; THIN; VERIFIED AT NECROPSY: NOT OBSERVED >BEHAVIOR; ATAXIC; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; LIMITED USE; LIMB-HINDRIGHT; VERIFIED AT NECROPSY: NOT APPLICABLE >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) THYMUS (TH) : -MISSING/NOT IDENTIFIED; NOT POSITIVELY IDENTIFIED; POSSIBLE REMNANTS COLLECTED HISTOPATHOLOGY BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT -M-ASTROCYTOMA,-NEOPLASM PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -NECROSIS, COAGULATIVE,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MINIMAL PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-MODERATE ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-MINIMAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),SLIGHT 1826 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 77 ANIMAL NUMBER: C92527 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/24/99 STUDY DAY OF DEATH: 584 STUDY WEEK OF DEATH: 84 TERMINAL BODY WEIGHT: 561.4 GRAMS DATE AND TIME OF NECROPSY: 11/24/99 8:24 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 11 X 8 X 5 MM, DARK RED, RED, TAN AND SOFT GENERAL COMMENT (GC) -BLOOD FILM TAKEN PARATHYROID (PT) : -HYPERPLASIA,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 1827 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 78 ANIMAL NUMBER: C92527 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/24/99 STUDY DAY OF DEATH: 584 STUDY WEEK OF DEATH: 84 TERMINAL BODY WEIGHT: 561.4 GRAMS DATE AND TIME OF NECROPSY: 11/24/99 8:24 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUm /s KULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 1828 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 79 ANIMAL NUMBER: C92528 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 430.7 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 10:32 PROSECTOR: DONIA MEYER RECORDER: KIMBERLY DURLAND POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT' (%) LF ADRENAL (AD0) RT ADRENAL (AD1) BRAIN (BR) LF KIDNEY (KD0) RT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LF TESTIS (TE0) RT TESTIS (TE1) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) .0347 .0315 2.2548 1.5393 1.6264 14.8186 1.8216 .6689 1.4100 1.4018 ..00111025 .0081 % .0073 % .5235 % .3574 % .3776 % 3.4406 % .4229 % .1553 % .3274 % .3255 % .0024 % .0026 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0154 .0140 1.0000 .6827 .7213 6.5720 .8079 .2967 .6253 .6217 .0047 .0050 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-MINIMAL KIDNEY (KD) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 1829 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 80 ANIMAL NUMBER: C92528 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 430.7 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 10:32 PROSECTOR: DONIA MEYER RECORDER: KIMBERLY DURLAND POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE) 1830 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 81 ANIMAL NUMBER: C92529 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 272.4 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 10:39 PROSECTOR: LYNN HORNER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN STATUS LF ADRENAL (AD0) RT ADRENAL (AD1) BRAIN (BR) LF KIDNEY (KD0) RT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LF TESTIS (TE0) RT TESTIS (TE1) LF THYROID/PARA RT THYROID/PARA (TT0) (TT1) ------------------------------------ 10.2021 ------------------------------------------- ------------------------------------ 3.7449 % ------------------------------------------- ------------------------------------------------------------------------------------- NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN WEIGHT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS 1831 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 82 ANIMAL NUMBER: C92529 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 272.4 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 10:39 PROSECTOR: LYNN HORNER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) 1832 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 83 ANIMAL NUMBER: C92530 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/18/99 STUDY DAY OF DEATH: 547 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: 597.0 GRAMS DATE AND TIME OF NECROPSY: 10/18/99 8:02 PROSECTOR: JARED FISHEL RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >RESPIRATION; LABORED; VERIFIED AT NECROPSY: NOT APPLICABLE >RESPIRATION; IRREGULAR; VERIFIED AT NECROPSY: NOT APPLICABLE >RESPIRATION; AUDIBLE; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; RECUMBENT; LATERAL; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; RED HAIR COAT; HEADENTIRE; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -RED FOCUS(I)/AREA(S); PAPILLARY PROCESS: SINGLE RED AREA, 3 MM IN DIAMETER; COLLECTED KIDNEY (KD) : -ROUGH SURFACE, DIFFUSE; BILATERAL HISTOPATHOLOGY BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL -ATROPHY, ACINAR,-MINIMAL ADRENAL, CORTEX (AC) : -PIGMENT,-MINIMAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATELY SEVERE LUNG (LU) : -MINERALIZATION, VASCULAR,-SLIGHT 1833 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 84 ANIMAL NUMBER: C92530 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/18/99 STUDY DAY OF DEATH: 547 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: 597.0 GRAMS DATE AND TIME OF NECROPSY: 10/18/99 8:02 PROSECTOR: JARED FISHEL RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 9 X 8 X 7 MM, RED, TAN, AND SOFT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: RED AND DRY MATERIAL -STAINS - SKIN/HAIRCOAT; ENTIRE HEAD: RED AND DRY MATERIAL HEART (HT) : -CARDIOMYOPATHY,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT URINARY BLADDER (UB) : -PROTEINACEOUS MATERIAL, LUMINAL,PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 1834 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 85 ANIMAL NUMBER: C92530 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/18/99 STUDY DAY OF DEATH: 547 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: 597.0 GRAMS DATE AND TIME OF NECROPSY: 10/18/99 8:02 PROSECTOR: JARED FISHEL RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUm /s KULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 1835 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 86 ANIMAL NUMBER: C92531 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/03/99 STUDY DAY OF DEATH: 471 STUDY WEEK OF DEATH: 68 TERMINAL BODY WEIGHT: 522.3 GRAMS DATE AND TIME OF NECROPSY: 08/03/99 8:06 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >BEHAVIOR; RECUMBENT; LATERAL; VERIFIED AT NECROPSY: NOT APPLICABLE >RESPIRATION; IRREGULAR; VERIFIED AT NECROPSY: NOT APPLICABLE >EXCRETION; NO FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >EYES; RED DISCHARGE; EYES; VERIFIED AT NECROPSY: OBSERVED >EYES; SQUINTED; PERI-ORBITAL; VERIFIED AT NECROPSY: NOT OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 9 X 8 X 7 MM, RED, DARK RED AND SOFT HISTOPATHOLOGY BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-MODERATE KIDNEY (KD) : -PIGMENT, TUBULAR,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL ADRENAL, CORTEX (AC) : -MINERALIZATION,-MINIMAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATELY SEVERE LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT 1836 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 87 ANIMAL NUMBER: C92531 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/03/99 STUDY DAY OF DEATH: 471 STUDY WEEK OF DEATH: 68 TERMINAL BODY WEIGHT: 522.3 GRAMS DATE AND TIME OF NECROPSY: 08/03/99 8:06 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA: MULTIPLE TAN ERODED AREAS, UP TO 6 X 4 MM GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DARK RED AND DRY MATERIAL STOMACH, NONGL (SU) : -ACANTHOSIS / HYPERKERATOSIS,-MODERATE -ULCERATION,-SLIGHT -INFLAMMATION, CHRONIC-ACTIVE,MODERATELY SEVERE PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 1837 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 88 ANIMAL NUMBER: C92531 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/03/99 STUDY DAY OF DEATH: 471 STUDY WEEK OF DEATH: 68 TERMINAL BODY WEIGHT: 522.3 GRAMS DATE AND TIME OF NECROPSY: 08/03/99 8:06 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUm /s KULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) (SN), 1838 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 89 ANIMAL NUMBER: C92532 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 581.4 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 13:54 PROSECTOR: JOHN S. HALFORD RECORDER: MATTHEW AXTMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: MATTHEW AXTMAN ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT' (%) LF ADRENAL (AD0) RT ADRENAL (AD1) BRAIN (BR) LF KIDNEY (KD0) RT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LF TESTIS (TE0) RT TESTIS (TE1) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) .0310 .0334 2.1402 2.2086 2.0147 17.2925 1.9369 .7755 1.5752 1.6085 .0336 .0310 .0053 % .0057 % .3681 % .3799 % .3465 % 2.9743 % .3331 % .1334 % .2709 % .2767 % .0058 % .0053 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0145 .0156 1.0000 1.0320 .9414 8.0799 .9050 .3623 .7360 .7516 .0157 .0145 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -NECROSIS, INDIVIDUAL HEPATOCYTE,MINIMAL KIDNEY (KD) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MINIMAL PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 1839 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 90 ANIMAL NUMBER: C92532 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 581.4 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 13:54 PROSECTOR: JOHN S. HALFORD RECORDER: MATTHEW AXTMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: MATTHEW AXTMAN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY LN, MANDIBULAR (MN) : -DIFFUSELY RED; RIGHT: DARK RED (CAP 21) LN, MANDIBULAR (MN) : >NOT REQUIRED TO BE EXAMINED FOR ANIMAL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG), GENERAL COMMENT (GC) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE) 1840 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 91 ANIMAL NUMBER: C92533 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/29/99 STUDY DAY OF DEATH: 589 STUDY WEEK OF DEATH: 85 TERMINAL BODY WEIGHT: 881.2 GRAMS DATE AND TIME OF NECROPSY: 11/29/99 15:37 PROSECTOR: TERRI L. WILLIAMS RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); INGUINAL REGION-LEFT; WEEK 69; 35 X 40MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); HEAD-MAXILLARY-RIGHT; WEEK 71; 30 X 40MM; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; HEAD MAXILLARY-RIGHT; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -DIFFUSELY LIGHT; ALL LOBES: TAN -RED FOCUS(I)/AREA(S); DIAPHRAGMATIC SURFACE OF RIGHT MEDIAN LOBE: MULTIPLE RED AREAS, UP TO 3 MM IN DIAMETER; SOME ARE SUNKEN WITH A STELLATE PATTERN; COLLECTED ON ROUTINE SECTIONS HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-SLIGHT -DEGENERATION, CYSTIC,-MINIMAL -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL -HYPERPLASIA, BILE DUCT,-SLIGHT -FIBROSIS, PERIBILIARY,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SLIGHT -HYALINE DROPLETS, TUBULAR EPITHELIUM,MODERATE SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-MINIMAL -HYPERPLASIA, CORTICAL,-SLIGHT -PIGMENT,-SLIGHT MARROW, FEMUR (FM) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT -HYPERPLASIA, MYELOID, MARROW,-MODERATE -N-FIBROSARCOMA,-NEOPLASM PRESENT 1841 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 92 ANIMAL NUMBER: C92533 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/29/99 STUDY DAY OF DEATH: 589 STUDY WEEK OF DEATH: 85 TERMINAL BODY WEIGHT: 881.2 GRAMS DATE AND TIME OF NECROPSY: 11/29/99 15:37 PROSECTOR: TERRI L. WILLIAMS RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY STOMACH, GL (ST) : -THICKENED WALL; MUCOSA, DIFFUSE PROSTATE (PR) : -NODULAR; DIFFUSE: GREEN AND FIRM LN, MANDIBULAR (MN) : -MOTTLED; BILATERAL: RED AND TAN; COLLECTED MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,-MODERATE LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL THYROID (TY) : -B-ADENOMA, FOLLICULAR CELL,-NEOPLASM PRESENT STOMACH, GL (ST) : -HYPERPLASIA, EPITHELIAL, GLANDULAR,SLIGHT SKIN (SK) : -M-FIBROSARCOMA,- 2 NEOPLASMS PRESENT PROSTATE (PR) : >UNREMARKABLE HEMATO NEOPLASIA (HN) : -M-SARCOMA, HISTIOCYTIC,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, SKIN/MUSCLE/SUBCUTIS,PRESENT LN, MANDIBULAR (MN) : -HYPERPLASIA, LYMPHOPLASMACYTIC,MODERATE MAMMARY, MALE (MM) : >NOT REQUIRED TO BE EXAMINED FOR ANIMAL >NOTE:>SEE SKIN FOR DIAGNOSIS 1842 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 93 ANIMAL NUMBER: C92533 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/29/99 STUDY DAY OF DEATH: 589 STUDY WEEK OF DEATH: 85 TERMINAL BODY WEIGHT: 881.2 GRAMS DATE AND TIME OF NECROPSY: 11/29/99 15:37 PROSECTOR: TERRI L. WILLIAMS RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY MAMMARY, MALE (MM) : -MASS(ES); LEFT INGUINAL REGION: SINGLE MASS, 44 X 40 X 18 MM, RED, TAN, AND FIRM; SAME ON CUT SECTION; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED -MASS #2; RIGHT CERVICAL REGION: SINGLE MASS, 38 X 30 X 20 MM, RED, DARK RED, TAN, AND FIRM; SAME ON CUT SECTION; COLLECTED; REGIONAL LN (SEE MANDIBULAR); OVERLYING SKIN HAS A SINGLE TAN ABRADED AREA; COLLECTED INTACT WITH MASS GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL OBESE 1843 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 94 ANIMAL NUMBER: C92533 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/29/99 STUDY DAY OF DEATH: 589 STUDY WEEK OF DEATH: 85 TERMINAL BODY WEIGHT: 881.2 GRAMS DATE AND TIME OF NECROPSY: 11/29/99 15:37 PROSECTOR: TERRI L. WILLIAMS RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUm /s KULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP) 1844 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 95 ANIMAL NUMBER: C92534 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 629.5 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 8:49 PROSECTOR: KEVIN BILLINGS RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT' (%) LF ADRENAL (AD0) RT ADRENAL (AD1) BRAIN (BR) LF KIDNEY (KD0) RT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LF TESTIS (TE0) RT TESTIS (TE1) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) .0341 .0350 2.2118 2.1935 2.3288 17.9201 1.9706 .9603 1.0650 2.0846 .0242 .0244 .0054 % .0056 % .3514 % .3485 % .3699 % 2.8467 % .3130 % .1525 % .1692 % .3312 % .0038 % .0039 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0154 .0158 1.0000 .9917 1.0529 8.1020 .8909 .4342 .4815 .9425 .0109 .0110 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: NOT OBSERVED LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL ADRENAL, CORTEX (AC) : -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL 1845 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 96 ANIMAL NUMBER: C92534 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 629.5 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 8:49 PROSECTOR: KEVIN BILLINGS RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY TESTIS (TE) : -SMALL; RIGHT: 20 X 12 X 6 MM (FOR COMPARISON, LEFT IS 14 X 10 X 10 MM); SOFT THYMUS (TH) : >NOTE:>TRIMMING COMMENT: THYMUS MISSING AT TIME OF TRIMMING LN, MANDIBULAR (MN) : -MOTTLED; TRIMMING COMMENT: DIFFUSELY RED AND TAN GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN BILATERAL: TESTIS (TE) : -ATROPHY/DEGENERATION,-MODERATE -GRANULOMA, SPERM,-PRESENT THYMUS (TH) : >TISSUE MISSING URINARY BLADDER (UB) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MANDIBULAR (MN) : -HEMORRHAGE,-PRESENT 1846 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 97 ANIMAL NUMBER: C92534 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 629.5 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 8:49 PROSECTOR: KEVIN BILLINGS RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA(PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC),VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE) MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU) ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), SEMINAL VESICLES (SV), HEMATO NEOPLASIA (HN) MARROW, FEMUR (FM), MUSCLE, SKELETAL (SM), PITUITARY (PI), DUODENUM (DU), JEJUNUM EPIDIDYMIDES (EP), (JE), 1847 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 98 ANIMAL NUMBER: C92535 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/14/00 STUDY DAY OF DEATH: 666 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: 747.0 GRAMS DATE AND TIME OF NECROPSY: 02/14/00 8:01 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >EXCRETION; DISCOLORED FECES; RED IN COLOR; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; TREMORS; LIMBS-ALL; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; COLD TO TOUCH; ENTIRE BODY; VERIFIED AT NECROPSY: NOT APPLICABLE >EXCRETION; NONFORMED FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >EYES; SQUINTED; PERI-ORBITAL; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) SPLEEN (SP) : -LIGHT FOCUS(I)/AREA(S); PARIETAL SURFACE OF CRANIAL END: SINGLE GRAY AREA, 15 X 10 MM -ADHESION(S); PARIETAL SURFACE ATTACHED TO GREATER CURVATURE OF STOMACH: MULTIPLE GRAY FIBROUS ADHESIONS; COLLECTED INTACT HISTOPATHOLOGY BRAIN (BR) : ^UNREMARKABLE LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, PERIPORTAL,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -PIGMENT, TUBULAR,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-SLIGHT -INFILTRATE, MACROPHAGE, PIGMENTED,SLIGHT -INFILTRATION, FAT, INTERSTITIAL,SLIGHT 1848 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 99 ANIMAL NUMBER: C92535 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/14/00 STUDY DAY OF DEATH: 666 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: 747.0 GRAMS DATE AND TIME OF NECROPSY: 02/14/00 8:01 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY TESTIS (TE) : -SMALL; LEFT: 15 X 5 X 3 MM (RIGHT IS 22 X 15 X 10 MM); LEFT IS SOFT PITUITARY (PI) : -MASS(ES); SINGLE MASS, 5 X 5 X 3 MM, RED, DARK RED, TAN, AND FIRM STOMACH, GL (ST) : -EROSION/ULCERATION; MUCOSA: MULTIPLE DARK BROWN, SLIGHTLY ERODED FOCI, UP TO 3 MM IN DIAMETER ILEUM (IL) : -DIFFUSELY RED; MUCOSA CECUM (CE) : -DIFFUSELY RED; MUCOSA ADRENAL, CORTEX (AC) : -PIGMENT,-SLIGHT ADRENAL, MEDULLA (MA) : -HYPERPLASIA, MEDULLARY,-SLIGHT TESTIS (TE) : -ATROPHY/DEGENERATION,-SEVERE >NOTE:>UNILATERAL BONE, STERNUM (SB) : -HYPERPLASIA, BONE,-MINIMAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATELY SEVERE THYROID (TY) : -M-CARCINOMA, FOLLICULAR CELL,-NEOPLASM PRESENT PITUITARY (PI) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE -NECROSIS,-SEVERE STOMACH, GL (ST) : -EDEMA,-MODERATE -EROSION,-MODERATE -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE ILEUM (IL) : -HEMORRHAGE,-PRESENT -NECROSIS,-SLIGHT CECUM (CE) : >UNREMARKABLE 1849 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 100 ANIMAL NUMBER: C92535 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/14/00 STUDY DAY OF DEATH: 666 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: 747.0 GRAMS DATE AND TIME OF NECROPSY: 02/14/00 8:01 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY RECTUM (RE) : -DIFFUSELY RED; MUCOSA ADIPOSE TISSUE (AT) : -MASS(ES); WITHIN ADIPOSE TISSUE, NEAR LEFT TESTIS: SINGLE MASS, 10 X 6 X 6 MM, YELLOW AND FIRM; SAME ON CUT SURFACE (CAP 22); REGIONAL LN (LUMBAR) NORMAL (CAP 23) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -GI TRACT-DARK FLUID CONTENT; ENTIRE GI TACT: DARK BROWN AND DARK RED FLUID MATERIAL RECTUM (RE) : -NECROSIS,-SEVERE EPIDIDYMIDES (EP) : -GRANULOMA, SPERM,-PRESENT CAUSE OF DEATH (CD) : -GI TRACT, EROSION/ULCERATION/HEMORRHAGE/INFLAMMA TION,-PRESENT LN, LUMBAR/ILIAC (LM) : -HYPERPLASIA, LYMPHOPLASMACYTIC,MODERATE ADIPOSE TISSUE (AT) : >NOT REQUIRED TO BE EXAMINED FOR ANIMAL >NOTE:>SEE EPIDIDYMIDES FOR DIAGNOSIS 1850 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 101 ANIMAL NUMBER: C92535 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/14/00 STUDY DAY OF DEATH: 666 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: 747.0 GRAMS DATE AND TIME OF NECROPSY: 02/14/00 8:01 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), COLON (CO), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAr /i LIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), COLON (CO), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), HEMATO NEOPLASIA (HN) (PT), 1851 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 102 ANIMAL NUMBER: C92536 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/17/99 STUDY DAY OF DEATH: 546 STUDY WEEK OF DEATH: 78 TERMINAL BODY WEIGHT: 808.5 GRAMS DATE AND TIME OF NECROPSY: 10/17/99 8:30 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >RESPIRATION; AUDIBLE; VERIFIED AT NECROPSY: NOT APPLICABLE >RESPIRATION; IRREGULAR; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; SWOLLEN; NOSE; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; COLD TO TOUCH; ENTIRE BODY; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY LUNG (LU) : -DIFFUSELY RED; ALL LOBES HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SLIGHT SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MINIMAL PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL -HYPERTROPHY / BASOPHILIA, ACINAR CELL, MINIMAL ADRENAL, CORTEX (AC) : -HYPERPLASIA, CORTICAL,-MINIMAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATE LUNG (LU) : >UNREMARKABLE THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : -HYPERPLASIA,-MINIMAL 1852 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 103 ANIMAL NUMBER: C92536 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/17/99 STUDY DAY OF DEATH: 546 STUDY WEEK OF DEATH: 78 TERMINAL BODY WEIGHT: 808.5 GRAMS DATE AND TIME OF NECROPSY: 10/17/99 8:30 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -LIGHT FOCUS(I)/AREA(S); LEFT ASPECT: SINGLE TAN FOCUS, 1 MM IN DIAMETER STOMACH, GL (ST) : -THICKENED WALL; MUCOSA SKIN (SK) : -RED FOCUS(I)/AREA(S); SUBCUTANEOUS TISSUE SURROUNDING MUZZLE: MULTIPLE INDISTINCT RED AND DARK RED AREAS; TISSUE SURROUNDING MUZZLE APPEARS SWOLLEN; ENTIRE MUZZLE COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-NASAL; DRY, RED MATERIAL -ANIMAL OBESE -DISCHARGE-ORAL; DRY, RED MATERIAL STOMACH, GL (ST) : -HYPERPLASIA, EPITHELIAL, GLANDULAR,SLIGHT SKIN (SK) : -ACANTHOSIS/HYPERKERATOSIS,-SLIGHT -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL -HEMORRHAGE,-PRESENT CAUSE OF DEATH (CD) : -POSSIBLE TRAUMATIC INJURY,-PRESENT 1853 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 104 ANIMAL NUMBER: C92536 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/17/99 STUDY DAY OF DEATH: 546 STUDY WEEK OF DEATH: 78 TERMINAL BODY WEIGHT: 808.5 GRAMS DATE AND TIME OF NECROPSY: 10/17/99 8:30 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 1854 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 105 ANIMAL NUMBER: C92537 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 02/14/00 STUDY DAY OF DEATH: 666 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 02/14/00 9:30 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); VENTRAL-CERVICAL-LEFT; WEEK 91; 45 X 50MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); DORSAL; WEEK 93; 15 X 15MM; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; DORSAL; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -DARK FOCUS(I)/AREA(S); DIAPHRAGMATIC SURFACE OF LEFT LATERAL LOBE: SINGLE DARK BROWN, SLIGHTLY DEPRESSED AREA, 8 X 8 X 1 MM; COLLECTED ON ROUTINE SECTION KIDNEY (KD) : -LIGHT FOCUS(I)/AREA(S); CORTEX OF RIGHT KIDNEY: SINGLE TAN FOCUS, 6 MM IN DIAMETER HISTOPATHOLOGY BRAIN (BR) : >UNREMARKABLE LIVER (LI) : -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-SLIGHT -HYPERTROPHY, HEPATOCELLULAR, PERIPORTAL,-MODERATE -DEGENERATION / NECROSIS, CENTRILOBULAR,-MODERATE KIDNEY (KD) : -N-SARCOMA,-NEOPLASM PRESENT SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : -VACUOLATION, DIFFUSE,-MODERATE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY) SEVERE LUNG (LU) : -N-SARCOMA,-NEOPLASM PRESENT 1855 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 106 ANIMAL NUMBER: C92537 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 02/14/00 STUDY DAY OF DEATH: 666 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 02/14/00 9:30 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY LUNG (LU) : -MASS(ES); ALL LOBES: MULTIPLE MASSES, UP TO 15 MM IN DIAMETER; RED, DARK RED, TAN, FIRM, AND LOBULATED; SAME ON CUT SURFACE; COLLECTED INTACT WITH LUNGS; REGIONAL LN (TRACHEOBRONCHIAL) NOT IDENTIFIED PITUITARY (PI) : -MASS(ES); SINGLE MASS, 6 X 5 X 4 MM, RED, DARK RED, TAN, LOBULATED, AND SOFT STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA: MULTIPLE TAN ERODED AREAS, UP TO 12 X 5 MM HEART (HT) : -N-SARCOMA,-NEOPLASM PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, NONGL (SU) : >UNREMARKABLE DUODENUM (DU) : >AUTOLYTIC JEJUNUM (JE) : >AUTOLYTIC ILEUM (IL) : >AUTOLYTIC CECUM (CE) : >AUTOLYTIC COLON (CO) : >AUTOLYTIC RECTUM (RE) : >AUTOLYTIC 1856 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 107 ANIMAL NUMBER: C92537 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 02/14/00 STUDY DAY OF DEATH: 666 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 02/14/00 9:30 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY SKIN (SK) : -MASS(ES); LEFT DORSAL SHOULDER REGION: SINGLE MASS, 15 X 13 X 5 MM, TAN AND BROWN; SAME ON CUT SURFACE; COLLECTED; OVERLYING SKIN HAS A DRY AND CRUSTED AREA, 15 X 13 MM; REGIONAL LN (AXILLARY) NORMAL (CAP 23) PROSTATE (PR) : -NODULAR; PROXIMAL, SURROUNDING URETHRA: GREEN, TAN, AND FIRM MAMMARY, MALE (MM) : -MASS(ES); LEFT CERVICAL REGION: SINGLE MASS, 65 X 50 X 35 MM, RED, DARK RED, GRAY, LOBULATED, AND VASCULAR; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (MANDIBULAR) NORMAL (CAP 22) SKIN (SK) : -B-KERATOACANTHOMA,-NEOPLASM PRESENT -M-FIBROSARCOMA,-NEOPLASM PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,MODERATELY SEVERE CAUSE OF DEATH (CD) : -NEOPLASM, SKIN/MUSCLE/SUBCUTIS,PRESENT MAMMARY, MALE (MM) : >NOT REQUIRED TO BE EXAMINED FOR ANIMAL >NOTE:>SEE SKIN FOR DIAGNOSIS OF MASS 1857 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 108 ANIMAL NUMBER: C92537 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 02/14/00 STUDY DAY OF DEATH: 666 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 02/14/00 9:30 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG), GENERAL COMMENT (GC) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN), LN, AXILLARY (AX), LN, MANDIBULAR (MN) 1858 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 109 ANIMAL NUMBER: C92538 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 09/14/99 STUDY DAY OF DEATH: 513 STUDY WEEK OF DEATH: 74 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 09/14/99 13:22 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; OBES; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-SLIGHT KIDNEY (KD) : -MINERALIZATION, PELVIC,-SLIGHT -MINERALIZATION, TUBULAR,-SLIGHT -INFLAMMATION, CHRONIC-ACTIVE, PELVIC,MODERATELY SEVERE PANCREAS (PA) : -B-ADENOMA, ISLET CELL,-NEOPLASM PRESENT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATE HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL ILEUM (IL) : >AUTOLYTIC CECUM (CE) : >AUTOLYTIC COLON (CO) : >AUTOLYTIC 1859 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 110 ANIMAL NUMBER: C92538 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 09/14/99 STUDY DAY OF DEATH: 513 STUDY WEEK OF DEATH: 74 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 09/14/99 13:22 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY URINARY BLADDER (UB) : -CALCULUS(I); WITHIN LUMEN: MULTIPLE TAN CALCULI, UP TO 6 MM IN DIAMETER GENERAL COMMENT (GC) : -URINARY BLADDER-LARGE LUMEN; MM IN DIAMETER -ANIMAL OBESE UP TO 30 URINARY BLADDER (UB) : -INFILTRATE, LYMPHOHISTIOCYTIC,MODERATELY SEVERE -HYPERPLASIA, TRANSITIONAL CELL,MODERATELY SEVERE -MINERALIZED MATERIAL, LUMEN,-PRESENT SEMINAL VESICLES (SV) : >AUTOLYTIC CAUSE OF DEATH (CD) : -INFECTION / NECROSIS / CALCULUS, URINARY TRACT,-PRESENT 1860 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 111 ANIMAL NUMBER: C92538 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 09/14/99 STUDY DAY OF DEATH: 513 STUDY WEEK OF DEATH: 74 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 09/14/99 13:22 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), RECTUM (RE), SKIN (SK), PROSTATE (PR), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 1861 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 112 ANIMAL NUMBER: C92539 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/28/00 STUDY DAY OF DEATH: 680 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: 517.8 GRAMS DATE AND TIME OF NECROPSY: 02/28/00 14:42 PROSECTOR: JARED FISHEL RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); TAIL-PROXIMAL; WEEK 87; VERIFIED AT NECROPSY: NOT OBSERVED >EYES; RED DISCHARGE; EYES; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; THIN; VERIFIED AT NECROPSY: NOT OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) KIDNEY (KD) : -ROUGH SURFACE, DIFFUSE; BILATERAL HISTOPATHOLOGY EYE (EY) : -DYSTROPHY, CORNEAL IN EARLY STAGES, CONSISTS PRIMARILY OF FOCAL MINERALIZATION,-MINIMAL BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -VACUOLATION, HEPATOCELLULAR,-SEVERE KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MODERATELY SEVERE -M-CARCINOMA, TUBULAR CELL,-NEOPLASM PRESENT SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,MODERATELY SEVERE -INFILTRATION, FAT, INTERSTITIAL,MODERATE 1862 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 113 ANIMAL NUMBER: C92539 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/28/00 STUDY DAY OF DEATH: 680 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: 517.8 GRAMS DATE AND TIME OF NECROPSY: 02/28/00 14:42 PROSECTOR: JARED FISHEL RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY TESTIS (TE) : -SMALL; BILATERAL: EACH IS 15 X 10 X 10 MM PITUITARY (PI) : -MASS(ES); 9 X 8 X 8 MM, DARK RED, TAN, AND SOFT STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA: MULTIPLE TAN ERODED AREAS, UP TO 2 MM IN DIAMETER PROSTATE (PR) : -NODULAR; PROXIMAL SURROUNDING URETHRA: GREEN AND FIRM ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-SLIGHT -PIGMENT,-MINIMAL TESTIS (TE) : -ATROPHY/DEGENERATION,-MINIMAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATELY SEVERE HEART (HT) : -CARDIOMYOPATHY,-MINIMAL -METAPLASIA, OSSEOUS / CARTILAGINOUS,SLIGHT THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, NONGL (SU) : -ACANTHOSIS / HYPERKERATOSIS,MODERATELY SEVERE -ULCERATION,-SLIGHT -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE PROSTATE (PR) : >UNREMARKABLE SEMINAL VESICLES (SV) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE 1863 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 114 ANIMAL NUMBER: C92539 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/28/00 STUDY DAY OF DEATH: 680 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: 517.8 GRAMS DATE AND TIME OF NECROPSY: 02/28/00 14:42 PROSECTOR: JARED FISHEL RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY TAIL (TI) : -CRUSTED AREA(S); PROXIMAL ONE-THIRD OF TAIL: MULTIPLE DARK BROWN, AND RAISED CRUSTED AREAS; COLLECTED WITH TAIL GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DRY RED MATERIAL -STAINS-PERINEUM/PERIANAL; EXTENDING TO MID ABDOMINAL REGION: MOIST BROWN MATERIAL SEMINAL VESICLES (SV) : -ATROPHY,-MODERATE EPIDIDYMIDES (EP) : -VACUOLATION, TUBULAR EPITHELIUM,MODERATE -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT TAIL (TI) : -INFLAMMATION, CHRONIC-ACTIVE,-SEVERE -ACANTHOSIS / HYPERKERATOSIS,-MODERATE 1864 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 115 ANIMAL NUMBER: C92539 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/28/00 STUDY DAY OF DEATH: 680 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: 517.8 GRAMS DATE AND TIME OF NECROPSY: 02/28/00 14:42 PROSECTOR: JARED FISHEL RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), HEMATO NEOPLASIA (HN) 1865 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 116 ANIMAL NUMBER: C92540 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 819.8 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 11:14 PROSECTOR: KEVIN BILLINGS RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT' (%) LF ADRENAL (AD0) RT ADRENAL (AD1) BRAIN (BR) LF KIDNEY (KD0) RT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LF TESTIS (TE0) RT TESTIS (TE1) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) .0399 .0359 2.2165 2.3557 2.4085 24.2163 2.7162 .8689 1.6604 1.5733 .0148 .0223 .0049 % .0044 % .2704 % .2874 % .2938 % 2.9539 % .3313 % .1060 % .2025 % .1919 % .0018 % .0027 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0180 .0162 1.0000 1.0628 1.0866 10.9255 1.2254 .3920 .7491 .7098 .0067 .0101 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,MODERATE -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-MINIMAL -VACUOLATION, HEPATOCELLULAR,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SLIGHT 1866 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 117 ANIMAL NUMBER: C92540 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 819.8 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 11:14 PROSECTOR: KEVIN BILLINGS RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY SPINAL CORD (SC) : >NOTE:>TRIMMING COMMENT: CERVICAL SPINAL CORD MISSING FROM WET TISSUE THYMUS (TH) : >NOTE:>TRIMMING COMMENT: MISSING FROM WET TISSUE PITUITARY (PI) : -RED FOCUS(I)/AREA(S); LEFT POSTERIOR ASPECT: SINGLE SLIGHTLY RAISED AREA, 2 MM IN DIAMETER GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN ADRENAL, CORTEX (AC) : -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL MARROW, STERNUM (SE) : -HYPOCELLULAR, MARROW,-SLIGHT SPINAL CORD (SC) : >UNREMARKABLE >NOTE:>TWO SEGMENTS EXAMINED THYMUS (TH) : >TISSUE MISSING LUNG (LU) : -HEMORRHAGE,-PRESENT THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT -ANGIECTASIS,-PRESENT PROSTATE (PR) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 1867 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 118 ANIMAL NUMBER: C92540 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 819.8 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 11:14 PROSECTOR: KEVIN BILLINGS RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAr /i LIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), BONE, STERNUM (SB), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 1868 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 119 ANIMAL NUMBER: C92541 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 709.4 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 12:34 PROSECTOR: JAMES T. CALLEN RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE DARK RED AREAS, UP TO 2 X 1 MM; COLLECTED ON ROUTINE SECTIONS HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL -DEGENERATION, CYSTIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, PELVIC,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MINIMAL PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-MODERATE -INFILTRATION, FAT, INTERSTITIAL,SLIGHT ADRENAL, CORTEX (AC) : -PIGMENT,-SLIGHT 1869 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 120 ANIMAL NUMBER: C92541 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 709.4 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 12:34 PROSECTOR: JAMES T. CALLEN RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY TESTIS (TE) : -SMALL; LEFT: 20 X 9 X 7 MM (RIGHT IS 26 X 15 X 15 MM); LEFT APPEARS SOFT PITUITARY (PI) : -MASS(ES); 10 X 7 X 6 MM, RED, DARK RED, TAN AND FIRM PROSTATE (PR) : -NODULAR; TISSUE SURROUNDING URETHRA GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN TESTIS (TE) : -ATROPHY/DEGENERATION,-SEVERE >NOTE:>UNILATERAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),SEVERE LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL HEART (HT) : -CARDIOMYOPATHY,-SLIGHT THYROID (TY) : -HYPERPLASIA, C-CELL,-SLIGHT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT URINARY BLADDER (UB) : -PROTEINACEOUS MATERIAL, LUMINAL,PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SEVERE CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 1870 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 121 ANIMAL NUMBER: C92541 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 709.4 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 12:34 PROSECTOR: JAMES T. CALLEN RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) (MS), THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 1871 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 122 ANIMAL NUMBER: C92542 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 792.2 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 10:16 PROSECTOR: TODD ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >WART LIKE LESION(S); HEAD-MAXILLARYRIGHT; WEEK 66; VERIFIED AT NECROPSY: OBSERVED >WART LIKE LESION(S); DORSAL-CERVICALRIGHT; WEEK 69; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); TAIL-PROXIMAL; WEEK 93; 15 X 10MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); DORSAL; WEEK 103; 10 X 10MM; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES, DIFFUSE: MULTIPLE DARK RED AREAS, UP TO 4 MM IN DIAMETER; COLLECTED ON ROUTINE SECTIONS HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MODERATE SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL -INFILTRATION, FAT, INTERSTITIAL,MODERATE ADRENAL, CORTEX (AC) : -B-ADENOMA,-NEOPLASM PRESENT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATE LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL 1872 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 123 ANIMAL NUMBER: C92542 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 792.2 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 10:16 PROSECTOR: TODD ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY STOMACH, GL (ST) : -THICKENED WALL; MUCOSA:: DIFFUSE SKIN (SK) : -CRUSTED AREA(S); RIGHT LATERAL CERVICAL REGION: SINGLE DARK RED, TAN, AND DRY AREA, 6 X 3 X 2 MM (CAP 24) -MASS(ES); RIGHT DORSAL THORACIC REGION: SINGLE MASS, 18 X 16 X 8 MM, TAN AND FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (AXILLARY) NORMAL (CAP 25) -RAISED FOCUS(I)/AREA(S); RIGHT SIDE OF MUZZLE: SINGLE DARK BROWN, TAN, AND DRY AREA, 3 X 3 X 8 MM (CAP 23) LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL -HYPERPLASIA, TYPE II PNEUMOCYTE,MINIMAL HEART (HT) : -CARDIOMYOPATHY,-MINIMAL STOMACH, GL (ST) : >UNREMARKABLE COLON (CO) : -PARASITISM,-PRESENT SKIN (SK) : -ACANTHOSIS/HYPERKERATOSIS,-MODERATELY SEVERE -INFLAMMATION, CHRONIC-ACTIVE,MODERATELY SEVERE -CYST, EPIDERMAL INCLUSION,-PRESENT -SUPERFICIAL CRUSTING,-MODERATELY SEVERE PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL SEMINAL VESICLES (SV) : -SECRETION, DECREASED,-PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 1873 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 124 ANIMAL NUMBER: C92542 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 792.2 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 10:16 PROSECTOR: TODD ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY LN, MANDIBULAR (MN) : -MOTTLED; BILATERAL: RED AND TAN; COLLECTED TAIL (TI) : -RAISED AREA(S); 40 MM FROM BASE: SINGLE TAN AREA, 12 X 7 X 3 MM; CUT SURFACE EXUDES A LIGHT GREEN SEMISOLID; COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN LN, MANDIBULAR (MN) : -HYPERPLASIA, LYMPHOPLASMACYTIC,MODERATE -HEMORRHAGE,-PRESENT TAIL (TI) : -CYST, EPIDERMAL INCLUSION,-PRESENT 1874 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 125 ANIMAL NUMBER: C92542 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 792.2 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 10:16 PROSECTOR: TODD ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), MAMMARY, MALE (MM), COAGULATING GL (CG), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), RECTUM (RE), URINARY BLADDER (UB), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN), LN, AXILLARY (AX) 1875 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 126 ANIMAL NUMBER: C92543 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/14/00 STUDY DAY OF DEATH: 695 STUDY WEEK OF DEATH: 100 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/14/00 7:06 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY PANCREAS (PA) : -MASS(ES); NEAR DUODENUM: SINGLE MASS, 4 MM IN DIAMETER, RED, TAN AND SOFT; SAME ON CUT SURFACE (CAP 22); REGIONAL LN (PANCREATIC) NOT IDENTIFIED PITUITARY (PI) : -DIFFUSELY RED; MID ASPECT: DARK RED HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, PERIPORTAL,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL PANCREAS (PA) : -M-CARCINOMA, ISLET CELL,-NEOPLASM PRESENT ADRENAL, CORTEX (AC) : -PIGMENT,-MINIMAL MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,-SLIGHT LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PARATHYROID (PT) : -HYPERPLASIA,-SLIGHT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT 1876 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 127 ANIMAL NUMBER: C92543 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/14/00 STUDY DAY OF DEATH: 695 STUDY WEEK OF DEATH: 100 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/14/00 7:06 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY ILEUM (IL) : >AUTOLYTIC CECUM (CE) : >AUTOLYTIC COLON (CO) : >AUTOLYTIC PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL CAUSE OF DEATH (CD) : -UNDETERMINED MICROSCOPICALLY,-PRESENT 1877 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 128 ANIMAL NUMBER: C92543 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/14/00 STUDY DAY OF DEATH: 695 STUDY WEEK OF DEATH: 100 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/14/00 7:06 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG), GENERAL COMMENT (GC) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 1878 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 129 ANIMAL NUMBER: C92544 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 531.6 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 11:14 PROSECTOR: TODD ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: NOT OBSERVED >WART LIKE LESION(S); PAW-HIND-LEFT; WEEK 95; VERIFIED AT NECROPSY: NOT OBSERVED >APPEARANCE; SWOLLEN; PAWS-HIND; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; MISSING; TEETH; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); LIMB-FRONT-LEFT; WEEK 102; 30 X 30MM; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; SWOLLEN; TAIL; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-SLIGHT -NECROSIS, INDIVIDUAL HEPATOCYTE,MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL -DEGENERATION, CYSTIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-MODERATE -FIBROSIS, PERIBILIARY,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SLIGHT PANCREAS (PA) : -ATROPHY, ACINAR,-MINIMAL -INFILTRATION, FAT, INTERSTITIAL,SLIGHT ADRENAL, CORTEX (AC) : -HYPERTROPHY, CORTICAL CELL,-MINIMAL -DEGENERATION, CYSTIC / HEMORRHAGIC,MINIMAL -PIGMENT,-SLIGHT ADRENAL, MEDULLA (MA) : -HYPERPLASIA, MEDULLARY,-SLIGHT -B-PHEOCHROMOCYTOMA,-NEOPLASM PRESENT 1879 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 130 ANIMAL NUMBER: C92544 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 531.6 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 11:14 PROSECTOR: TODD ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY THYMUS (TH) : -MISSING/NOT IDENTIFIED; NOT POSSIBLY IDENTIFIED, POSSIBLE REMNANTS COLLECTED MUSCLE, SKELETAL (SM) : -MASS(ES); MUSCLE SURROUNDING LEFT FRONT LEG: SINGLE MASS, 40 X 20 X 25 MM, TAN, RED, DARK RED, GREY, AND FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (AXILLARY) NORMAL (CAP 25) PITUITARY (PI) : -RED FOCUS(I)/AREA(S); RIGHT ANTERIOR ASPECT: SINGLE DARK RED FOCUS, 3 MM IN DIAMETER PROSTATE (PR) : -NODULAR; PROXIMAL SURROUNDING URETHRA: GREEN AND FIRM LN, MANDIBULAR (MN) : -LARGE; BILATERAL: UP TO 11 X 7 X 3 MM; COLLECTED THYMUS (TH) : >SECTION EXAMINED; TISSUE NOT PRESENT LUNG (LU) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL HEART (HT) : -CARDIOMYOPATHY,-MINIMAL MUSCLE, SKELETAL (SM) : -M-SARCOMA, UNDIFFERENTIATED,-NEOPLASM PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE SEMINAL VESICLES (SV) : -SECRETION, DECREASED,-PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MANDIBULAR (MN) : -HYPERPLASIA, LYMPHOPLASMACYTIC,MODERATELY SEVERE -LYMPHANGIECTASIS,-PRESENT 1880 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 131 ANIMAL NUMBER: C92544 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 531.6 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 11:14 PROSECTOR: TODD ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY TAIL (TI) : -LARGE; ENTIRE TAIL: UP TO 15 MM IN DIAMETER; COLLECTED LN, LUMBAR/ILIAC (LM) : -LARGE; BILATERAL: LEFT IS 7 X 6 X 4 MM, RIGHT IS 24 X 22 X 10 MM; BOTH ARE CYSTIC (CAP 24) JOINT, OTHER (JT) : -LARGE; BILATERAL TARSAL JOINT: BOTH ARE 13 MM IN DIAMETER; BOTH COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -TEETH MISSING/BROKEN/WORN; BILATERAL UPPER INCISORS: BROKEN OFF AT BASE TAIL (TI) : -ACANTHOSIS / HYPERKERATOSIS,-SLIGHT LN, LUMBAR/ILIAC (LM) : -LYMPHANGIECTASIS,-PRESENT JOINT, OTHER (JT) : -INFLAMMATION, CHRONIC-ACTIVE,MODERATELY SEVERE -FIBROSIS,-SEVERE 1881 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 132 ANIMAL NUMBER: C92544 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 531.6 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 11:14 PROSECTOR: TODD ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), MAMMARY, MALE (MM), COAGULATING GL (CG), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN), LN, AXILLARY (AX) 1882 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 133 ANIMAL NUMBER: C92545 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 01/31/00 STUDY DAY OF DEATH: 652 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 01/31/00 8:40 PROSECTOR: STEVE VAN ADESTINE RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY HISTOPATHOLOGY LIVER (LI) : -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -DILATATION, PELVIC,-MINIMAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SLIGHT PANCREAS (PA) : -ATROPHY, ACINAR,-MINIMAL ADRENAL, CORTEX (AC) : -HYPERTROPHY, CORTICAL CELL,-SLIGHT -PIGMENT,-SLIGHT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATELY SEVERE HEART (HT) : -CARDIOMYOPATHY,-SLIGHT PITUITARY (PI) : -HYPERPLASIA,-MODERATE CECUM (CE) : >AUTOLYTIC 1883 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 134 ANIMAL NUMBER: C92545 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 01/31/00 STUDY DAY OF DEATH: 652 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 01/31/00 8:40 PROSECTOR: STEVE VAN ADESTINE RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS COLON (CO) : >AUTOLYTIC CAUSE OF DEATH (CD) : -UNDETERMINED MICROSCOPICALLY,-PRESENT 1884 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 135 ANIMAL NUMBER: C92545 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 01/31/00 STUDY DAY OF DEATH: 652 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 01/31/00 8:40 PROSECTOR: STEVE VAN ADESTINE RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 1885 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 136 ANIMAL NUMBER: C92546 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 317.9 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 12:03 PROSECTOR: LYNN HORNER RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN STATUS LF ADRENAL (AD0) RT ADRENAL (AD1) BRAIN (BR) LF KIDNEY (KD0) RT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LF TESTIS (TE0) RT TESTIS (TE1) LF THYROID/PARA RT THYROID/PARA (TT0) (TT1) ------------------------------------ 11.0070 ------------------------------------------- ------------------------------------ 3.4623 % ------------------------------------------- ------------------------------------------------------------------------------------- NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN WEIGHT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS 1886 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 137 ANIMAL NUMBER: C92546 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 317.9 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 12:03 PROSECTOR: LYNN HORNER RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) 1887 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 138 ANIMAL NUMBER: C92547 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/21/99 STUDY DAY OF DEATH: 611 STUDY WEEK OF DEATH: 88 TERMINAL BODY WEIGHT: 627.2 GRAMS DATE AND TIME OF NECROPSY: 12/21/99 7:35 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; ROUGH HAIR COAT; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; THIN; VERIFIED AT NECROPSY: NOT OBSERVED >EXCRETION; NO FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; HEAD TILT; RIGHT; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; YELLOW HAIR COAT; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; ATAXIC; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -ROUGH SURFACE, DIFFUSE; ALL LOBES SPLEEN (SP) : -LARGE; 60 X 18 X 10 MM TESTIS (TE) : -SMALL; BILATERAL: EACH IS 20 X 12 X 6 MM; BOTH ARE SOFT HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT LIVER (LI) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SLIGHT -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT SPLEEN (SP) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL ADRENAL, CORTEX (AC) : -B-ADENOMA,-NEOPLASM PRESENT -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT TESTIS (TE) : -ATROPHY/DEGENERATION,-SEVERE 1888 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 139 ANIMAL NUMBER: C92547 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/21/99 STUDY DAY OF DEATH: 611 STUDY WEEK OF DEATH: 88 TERMINAL BODY WEIGHT: 627.2 GRAMS DATE AND TIME OF NECROPSY: 12/21/99 7:35 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 9 X 7 X 6 MM, RED, DARK RED, AND SOFT MARROW, FEMUR (FM) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT -HYPERPLASIA, MYELOID, MARROW,-MODERATE MARROW, STERNUM (SE) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT -HYPERPLASIA, MYELOID, MARROW,-MODERATE SPINAL CORD (SC) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),SEVERE LUNG (LU) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT HEART (HT) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT THYROID (TY) : -DILATATION / CYST, FOLLICULAR,-PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : >UNREMARKABLE 1889 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 140 ANIMAL NUMBER: C92547 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/21/99 STUDY DAY OF DEATH: 611 STUDY WEEK OF DEATH: 88 TERMINAL BODY WEIGHT: 627.2 GRAMS DATE AND TIME OF NECROPSY: 12/21/99 7:35 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: MULTIPLE BROWN AREAS, UP TO 1 MM IN DIAMETER STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA: MULTIPLE TAN ERODED AREAS, UP TO 5 X 4 MM PROSTATE (PR) : -NODULAR; PROXIMAL SURROUNDING URETHRA: GREEN AND FIRM SEMINAL VESICLES (SV) : -SMALL; BILATERAL: EACH IS 15 X 10 X 5 MM GENERAL COMMENT (GC) : -BLOOD FILM TAKEN STOMACH, NONGL (SU) : -ACANTHOSIS / HYPERKERATOSIS,-MODERATE -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE SEMINAL VESICLES (SV) : -SECRETION, DECREASED,-PRESENT HEMATO NEOPLASIA (HN) : -M-LYMPHOMA,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, HEMATOPOIETIC,-PRESENT 1890 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 141 ANIMAL NUMBER: C92547 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/21/99 STUDY DAY OF DEATH: 611 STUDY WEEK OF DEATH: 88 TERMINAL BODY WEIGHT: 627.2 GRAMS DATE AND TIME OF NECROPSY: 12/21/99 7:35 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), BONE, STERNUM (SB), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP) 1891 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 142 ANIMAL NUMBER: C92548 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/22/99 STUDY DAY OF DEATH: 582 STUDY WEEK OF DEATH: 84 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/22/99 10:10 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 13 X 10 X 8 MM, DARK RED, TAN AND SOFT HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-SLIGHT ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MINIMAL -PIGMENT,-MINIMAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),SEVERE LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT JEJUNUM (JE) : >AUTOLYTIC ILEUM (IL) : >AUTOLYTIC 1892 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 143 ANIMAL NUMBER: C92548 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/22/99 STUDY DAY OF DEATH: 582 STUDY WEEK OF DEATH: 84 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/22/99 10:10 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PROSTATE (PR) : -NODULAR; PROXIMAL, SURROUNDING URETHRA: GREEN AND FIRM CECUM (CE) : >AUTOLYTIC PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 1893 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 144 ANIMAL NUMBER: C92548 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/22/99 STUDY DAY OF DEATH: 582 STUDY WEEK OF DEATH: 84 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/22/99 10:10 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUm /s KULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG), GENERAL COMMENT (GC) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), KIDNEY (KD), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 1894 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 145 ANIMAL NUMBER: C92549 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 10/31/99 STUDY DAY OF DEATH: 560 STUDY WEEK OF DEATH: 80 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 10/31/99 14:51 PROSECTOR: TROY ELLENBECKER RECORDER: TROY ELLENBECKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS HISTOPATHOLOGY LIVER (LI) : -FIBROSIS, PERIBILIARY,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL PANCREAS (PA) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL ADRENAL, MEDULLA (MA) : -UNILATERALLY EXAMINED,-PRESENT TESTIS (TE) : -ATROPHY/DEGENERATION,-SEVERE >NOTE:>UNILATERAL THYMUS (TH) : -HEMORRHAGE,-PRESENT -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATELY SEVERE CECUM (CE) : >AUTOLYTIC CAUSE OF DEATH (CD) : -UNDETERMINED MICROSCOPICALLY,-PRESENT 1895 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 146 ANIMAL NUMBER: C92549 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 10/31/99 STUDY DAY OF DEATH: 560 STUDY WEEK OF DEATH: 80 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 10/31/99 14:51 PROSECTOR: TROY ELLENBECKER RECORDER: TROY ELLENBECKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), ADRENAL, CORTEX (AC), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 1896 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 147 ANIMAL NUMBER: C92550 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 04/15/00 STUDY DAY OF DEATH: 727 STUDY WEEK OF DEATH: 104 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 04/15/00 8:15 PROSECTOR: JARED FISHEL RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; SWOLLEN; PAW-HIND-RIGHT; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -HYPERPLASIA, BILE DUCT,-SLIGHT -FIBROSIS, PERIBILIARY,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SLIGHT ADRENAL, CORTEX (AC) : -PIGMENT,-SLIGHT ADRENAL, MEDULLA (MA) : -B-PHEOCHROMOCYTOMA,-NEOPLASM PRESENT LN, MESENTERIC (MS) : -HEMORRHAGE,-PRESENT TESTIS (TE) : -MINERALIZATION, VASCULAR,-MINIMAL 1897 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 148 ANIMAL NUMBER: C92550 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 04/15/00 STUDY DAY OF DEATH: 727 STUDY WEEK OF DEATH: 104 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 04/15/00 8:15 PROSECTOR: JARED FISHEL RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY FOOT/FOOTPAD (FP) : -LARGE; RIGHT HINDFOOT: 45 X 14 X 12 MM (LEFT IS 45 X 10 X 8 MM); RIGHT APPEARS SWOLLEN; COLLECTED THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATELY SEVERE LUNG (LU) : -EDEMA, ALVEOLAR,-SLIGHT HEART (HT) : -CARDIOMYOPATHY,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT CECUM (CE) : >AUTOLYTIC PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT CAUSE OF DEATH (CD) : -UNDETERMINED MICROSCOPICALLY,-PRESENT FOOT/FOOTPAD (FP) : >UNREMARKABLE 1898 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 149 ANIMAL NUMBER: C92550 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 04/15/00 STUDY DAY OF DEATH: 727 STUDY WEEK OF DEATH: 104 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 04/15/00 8:15 PROSECTOR: JARED FISHEL RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG), GENERAL COMMENT (GC) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 1899 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 150 ANIMAL NUMBER: C92551 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 674.3 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 8:15 PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-SLIGHT -VACUOLATION, HEPATOCELLULAR,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MODERATE -MINERALIZATION, PELVIC,-MODERATE -HYPERPLASIA, TRANSITIONAL CELL,MINIMAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE PANCREAS (PA) : -INFILTRATION, FAT, INTERSTITIAL,SLIGHT ADRENAL, CORTEX (AC) : -HYPERTROPHY, CORTICAL CELL,-SEVERE -PIGMENT,-SLIGHT 1900 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 151 ANIMAL NUMBER: C92551 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 674.3 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 8:15 PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY THYROID (TY) : -LARGE; RIGHT: 6 X 4 X 2 MM -SMALL; LEFT: 2 X 2 X 1 MM PITUITARY (PI) : -MASS(ES); 8 X 6 X 5 MM, DARK RED, TAN AND SOFT STOMACH, GL (ST) : -THICKENED WALL; DIFFUSE PROSTATE (PR) : -NODULAR; PROXIMAL, SURROUNDING URETHRA: GREEN AND FIRM LN, AXILLARY (AX) : -MOTTLED; BILATERAL: BROWN AND TAN (CAP 23) ADRENAL, MEDULLA (MA) : -HYPERPLASIA, MEDULLARY,-MINIMAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATELY SEVERE HEART (HT) : -CARDIOMYOPATHY,-MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-SLIGHT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : -HYPERPLASIA, EPITHELIAL, GLANDULAR,SLIGHT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, AXILLARY (AX) : -INFILTRATE, MACROPHAGE, PIGMENTED,SLIGHT MAMMARY, MALE (MM) : -GALACTOCELE,-PRESENT -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE 1901 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 152 ANIMAL NUMBER: C92551 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 674.3 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 8:15 PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY MAMMARY, MALE (MM) : -MASS(ES); RIGHT AXILLARY REGION: SINGLE MASS, 12 X 12 X 9 MM, DARK RED, TAN AND FIRM; CUT SURFACE EXUDES A TAN CREAMY SEMIFLUID; COLLECTED; REGIONAL LN (SEE AXILLARY) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN 1902 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 153 ANIMAL NUMBER: C92551 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 674.3 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 8:15 PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 1903 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 154 ANIMAL NUMBER: C92552 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/14/00 STUDY DAY OF DEATH: 726 STUDY WEEK OF DEATH: 104 TERMINAL BODY WEIGHT: 450.2 GRAMS DATE AND TIME OF NECROPSY: 04/14/00 7:35 PROSECTOR: JARED FISHEL RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; HEAD TILT; RIGHT; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; THIN; VERIFIED AT NECROPSY: NOT OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) TESTIS (TE) : -SMALL; BILATERAL: EACH IS 15 X 11 X 7 MM; BOTH ARE SOFT HISTOPATHOLOGY EYE (EY) : -DYSTROPHY, CORNEAL IN EARLY STAGES, CONSISTS PRIMARILY OF FOCAL MINERALIZATION,-MINIMAL BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MODERATE SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,MODERATELY SEVERE ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC / HEMORRHAGIC,MINIMAL -PIGMENT,-MINIMAL TESTIS (TE) : -ATROPHY/DEGENERATION,-MODERATELY SEVERE 1904 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 155 ANIMAL NUMBER: C92552 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/14/00 STUDY DAY OF DEATH: 726 STUDY WEEK OF DEATH: 104 TERMINAL BODY WEIGHT: 450.2 GRAMS DATE AND TIME OF NECROPSY: 04/14/00 7:35 PROSECTOR: JARED FISHEL RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 11 MM IN DIAMETER, DARK RED, GRAY AND SOFT PROSTATE (PR) : -MASS(ES); RIGHT PROXIMAL REGION, NEAR SEMINAL VESICLE: SINGLE MASS, 12 X 10 X 4 MM, GRAY, TAN AND SOFT; COLLECTED INTACT WITH PROSTATE; REGIONAL LN (LUMBAR) NORMAL (CAP 22) TESTIS (TE) : -MINERALIZATION, VASCULAR,-MINIMAL -B-INTERSTITIAL CELL TUMOR,-NEOPLASM PRESENT -INFILTRATE, LYMPHOHISTIOCYTIC,-SLIGHT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),SEVERE HEART (HT) : -CARDIOMYOPATHY,-SLIGHT THYROID (TY) : -HYPERPLASIA, C-CELL,-SLIGHT -DILATATION / CYST, FOLLICULAR,-PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,MODERATELY SEVERE -HYPERPLASIA, EPITHELIAL,-MODERATELY SEVERE EPIDIDYMIDES (EP) : -HYPOSPERMIA,-PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 1905 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 156 ANIMAL NUMBER: C92552 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/14/00 STUDY DAY OF DEATH: 726 STUDY WEEK OF DEATH: 104 TERMINAL BODY WEIGHT: 450.2 GRAMS DATE AND TIME OF NECROPSY: 04/14/00 7:35 PROSECTOR: JARED FISHEL RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; RIGHT: DRY RED MATERIAL LN, LUMBAR/ILIAC (LM) : -HYPERPLASIA, LYMPHOPLASMACYTIC,MODERATE 1906 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 157 ANIMAL NUMBER: C92552 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/14/00 STUDY DAY OF DEATH: 726 STUDY WEEK OF DEATH: 104 TERMINAL BODY WEIGHT: 450.2 GRAMS DATE AND TIME OF NECROPSY: 04/14/00 7:35 PROSECTOR: JARED FISHEL RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUm /s KULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), HEMATO NEOPLASIA (HN) 1907 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 158 ANIMAL NUMBER: C92553 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/28/99 STUDY DAY OF DEATH: 435 STUDY WEEK OF DEATH: 63 TERMINAL BODY WEIGHT: 344.7 GRAMS DATE AND TIME OF NECROPSY: 06/28/99 14:38 PROSECTOR: KEVIN BILLINGS RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; ROUGH HAIR COAT; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; BROWN HAIR COAT; PERINEAL AREA; VERIFIED AT NECROPSY: NOT OBSERVED >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; SWOLLEN; VENTRAL ABDOMINAL; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY ESOPHAGUS (ES) : -LARGE; DIFFUSE: DIAMETER TO 8 MM IN HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : -PIGMENT,-MINIMAL MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,-SLIGHT LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL -INFILTRATE, MACROPHAGE, ALVEOLAR,SLIGHT -INFLAMMATION, GRANULOMATOUS,-MINIMAL ESOPHAGUS (ES) : -DILATATION, LUMINAL,-PRESENT PARATHYROID (PT) : >TISSUE MISSING PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL CAUSE OF DEATH (CD) : -UNDETERMINED MICROSCOPICALLY,-PRESENT 1908 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 159 ANIMAL NUMBER: C92553 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/28/99 STUDY DAY OF DEATH: 435 STUDY WEEK OF DEATH: 63 TERMINAL BODY WEIGHT: 344.7 GRAMS DATE AND TIME OF NECROPSY: 06/28/99 14:38 PROSECTOR: KEVIN BILLINGS RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL, LOWER INCISORS -GI TRACT-LARGE LUMEN; DUODENUM, JEJUNUM, ILEUM AND CECUM: DUODENUM IS UP TO 10 MM IN DIAMETER; JEJUNUM, ILEUM AND CECUM ARE UP TO 20 MM IN DIAMETER; ALL ARE DISTENDED WITH GAS CAUSE OF DEATH (CD) : >NOTE:>DILATED ESOPHAGUS MAY SUGGEST OBSTRUCTION, WITH SOME ASPIRATION RESULTING IN THE GRANULOMATOUS CHANGES IN THE LUNG. 1909 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 160 ANIMAL NUMBER: C92553 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/28/99 STUDY DAY OF DEATH: 435 STUDY WEEK OF DEATH: 63 TERMINAL BODY WEIGHT: 344.7 GRAMS DATE AND TIME OF NECROPSY: 06/28/99 14:38 PROSECTOR: KEVIN BILLINGS RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAr /i LIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), THYROID (TY), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 1910 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 161 ANIMAL NUMBER: C92554 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/07/00 STUDY DAY OF DEATH: 719 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 510.8 GRAMS DATE AND TIME OF NECROPSY: 04/07/00 14:38 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >EXCRETION; NO FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; RED HAIR COAT; HEADENTIRE; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; RED HAIR COAT; VENTRAL ABDOMINAL; VERIFIED AT NECROPSY: OBSERVED >EYES; SQUINTED; PERI-ORBITAL; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; RECUMBENT; STERNAL; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -DARK FOCUS(I)/AREA(S); LEFT LATERAL LOBE: MULTIPLE DARK BROWN AREAS, UP TO 4 X 3 MM; SOME AREAS HAVE A STELLATE PATTERN; COLLECTED ON ROUTINE SECTION HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -HEMORRHAGE,-MINIMAL -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-MODERATE -HEMATOPOIESIS, EXTRAMEDULLARY,-SLIGHT -HYPERPLASIA, BILE DUCT,-MINIMAL -FIBROSIS, PERIBILIARY,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : -PIGMENT,-MINIMAL MARROW, FEMUR (FM) : -HYPOCELLULAR, MARROW,-SEVERE MARROW, STERNUM (SE) : -HYPOCELLULAR, MARROW,-MODERATELY SEVERE 1911 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 162 ANIMAL NUMBER: C92554 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/07/00 STUDY DAY OF DEATH: 719 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 510.8 GRAMS DATE AND TIME OF NECROPSY: 04/07/00 14:38 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 10 X 9 X 7 MM, RED, DARK RED, GREY, TAN AND SOFT STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA, SURROUNDING PYLORIC VALVE: MULTIPLE BROWN AREAS, UP TO 3 X 1 MM STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA, SURROUNDING CARDIAC VALVE: MULTIPLE TAN AND SLIGHTLY ERODED AREAS, UP TO 3 X 1 MM PROSTATE (PR) : -NODULAR; PROXIMAL, TISSUE SURROUNDING URETHRA: GREEN SEMINAL VESICLES (SV) : -SMALL; BILATERAL: EACH IS 14 X 5 X 4 MM GENERAL COMMENT (GC) : -BLOOD FILM TAKEN THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATELY SEVERE HEART (HT) : -CARDIOMYOPATHY,-SLIGHT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : -EROSION,-SLIGHT -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT STOMACH, NONGL (SU) : -ACANTHOSIS / HYPERKERATOSIS,-MODERATE -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE SEMINAL VESICLES (SV) : -ATROPHY,-SEVERE CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 1912 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 163 ANIMAL NUMBER: C92554 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/07/00 STUDY DAY OF DEATH: 719 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 510.8 GRAMS DATE AND TIME OF NECROPSY: 04/07/00 14:38 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -STAINS - SKIN/HAIRCOAT; ALL REGIONS, DIFFUSE: DRY, RED MATERIAL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAr /i LIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES HEMATO NEOPLASIA (HN) (EP), 1913 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 164 ANIMAL NUMBER: C92555 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/08/99 STUDY DAY OF DEATH: 415 STUDY WEEK OF DEATH: 60 TERMINAL BODY WEIGHT: 540.6 GRAMS DATE AND TIME OF NECROPSY: 06/08/99 14:30 PROSECTOR: GREGORY CODDINGTON RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; YELLOW HAIR COAT; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; NO FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; ROUGH HAIR COAT; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; HUNCHED; VERIFIED AT NECROPSY: NOT APPLICABLE >EYES; RED DISCHARGE; EYE-RIGHT; VERIFIED AT NECROPSY: NOT OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) HISTOPATHOLOGY BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-MODERATELY SEVERE KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-MODERATE -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL -HYPERPLASIA, ISLET CELLS,-MINIMAL ADRENAL, CORTEX (AC) : -PIGMENT,-MINIMAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),SEVERE HEART (HT) : -CARDIOMYOPATHY,-MINIMAL 1914 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 165 ANIMAL NUMBER: C92555 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/08/99 STUDY DAY OF DEATH: 415 STUDY WEEK OF DEATH: 60 TERMINAL BODY WEIGHT: 540.6 GRAMS DATE AND TIME OF NECROPSY: 06/08/99 14:30 PROSECTOR: GREGORY CODDINGTON RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 12 X 8 X 8 MM, RED, TAN AND SOFT STOMACH, NONGL (SU) : -THICKENED WALL; DIFFUSE RED, DARK GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; LEFT: DARK RED AND DRY -STAINS-PERINEUM/PERIANAL; MOIST YELLOW MATERIAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, NONGL (SU) : -ACANTHOSIS / HYPERKERATOSIS,-MODERATE -INFLAMMATION, CHRONIC-ACTIVE,MODERATELY SEVERE PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 1915 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 166 ANIMAL NUMBER: C92555 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/08/99 STUDY DAY OF DEATH: 415 STUDY WEEK OF DEATH: 60 TERMINAL BODY WEIGHT: 540.6 GRAMS DATE AND TIME OF NECROPSY: 06/08/99 14:30 PROSECTOR: GREGORY CODDINGTON RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUm /s KULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) (SN), 1916 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 167 ANIMAL NUMBER: C92556 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/28/99 STUDY DAY OF DEATH: 435 STUDY WEEK OF DEATH: 63 TERMINAL BODY WEIGHT: 402.2 GRAMS DATE AND TIME OF NECROPSY: 06/28/99 14:33 PROSECTOR: JERALD W. JOHNSON RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -MOTTLED; ALL LOBES: RED AND TAN TESTIS (TE) : -SMALL; BILATERAL: EACH IS 17 X 9 X 8 MM HISTOPATHOLOGY BRAIN (BR) : >UNREMARKABLE LIVER (LI) : -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL -GLYCOGEN, HEPATOCELLULAR, INCREASED,MODERATELY SEVERE KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SLIGHT -HYALINE DROPLETS, TUBULAR EPITHELIUM,MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE PANCREAS (PA) : ^UNREMARKABLE >NOTE:>SEE SLIDE #17 ADRENAL, CORTEX (AC) : -HYPERTROPHY, CORTICAL CELL,-MINIMAL -PIGMENT,-MINIMAL TESTIS (TE) : -ATROPHY/DEGENERATION,-MODERATE 1917 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 168 ANIMAL NUMBER: C92556 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/28/99 STUDY DAY OF DEATH: 435 STUDY WEEK OF DEATH: 63 TERMINAL BODY WEIGHT: 402.2 GRAMS DATE AND TIME OF NECROPSY: 06/28/99 14:33 PROSECTOR: JERALD W. JOHNSON RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 11 X 9 X 7 MM, RED, DARK RED, VASCULAR AND SOFT SEMINAL VESICLES (SV) : -SMALL; BILATERAL: EACH IS 10 X 4 X 1 MM GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL, UPPER INCISORS -ANIMAL THIN THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATE HEART (HT) : -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, NONGL (SU) : -ACANTHOSIS / HYPERKERATOSIS,-MODERATE -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT SEMINAL VESICLES (SV) : -SECRETION, DECREASED,-PRESENT EPIDIDYMIDES (EP) : -HYPOSPERMIA,-PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 1918 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 169 ANIMAL NUMBER: C92556 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/28/99 STUDY DAY OF DEATH: 435 STUDY WEEK OF DEATH: 63 TERMINAL BODY WEIGHT: 402.2 GRAMS DATE AND TIME OF NECROPSY: 06/28/99 14:33 PROSECTOR: JERALD W. JOHNSON RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), HEMATO NEOPLASIA (HN) 1919 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 170 ANIMAL NUMBER: C92557 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/27/99 STUDY DAY OF DEATH: 617 STUDY WEEK OF DEATH: 89 TERMINAL BODY WEIGHT: 486.8 GRAMS DATE AND TIME OF NECROPSY: 12/27/99 14:09 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; ROUGH HAIR COAT; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; HUNCHED; VERIFIED AT NECROPSY: NOT APPLICABLE >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; RED HAIR COAT; VENTRAL ABDOMINAL; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) TESTIS (TE) : -CONTAINS FLUID; BILATERAL: BOTH ARE SOFT AND GELATINOUS -RED/YELLOW FOCUS(I)/AREA(S); RIGHT, BENEATH TUNICA: SINGLE YELLOW AREA, 8 X 4 MM HISTOPATHOLOGY BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-MODERATE -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL -ATROPHY, ACINAR,-MINIMAL -B-ADENOMA, ISLET CELL,-NEOPLASM PRESENT TESTIS (TE) : -ATROPHY/DEGENERATION,-SEVERE -GRANULOMA, SPERM,-PRESENT >NOTE:>BILATERAL 1920 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 171 ANIMAL NUMBER: C92557 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/27/99 STUDY DAY OF DEATH: 617 STUDY WEEK OF DEATH: 89 TERMINAL BODY WEIGHT: 486.8 GRAMS DATE AND TIME OF NECROPSY: 12/27/99 14:09 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 10 X 9 X 8 MM, RED, DARK RED, TAN, GRAY AND SOFT STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA: MULTIPLE TAN ERODED AREAS, UP TO 3 MM IN DIAMETER PROSTATE (PR) : -NODULAR; PROXIMAL, SURROUNDING URETHRA: GREEN AND FIRM GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN -STAINS - SKIN/HAIRCOAT; VENTRAL ABDOMINAL REGION: DARK RED AND MOIST THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),SEVERE THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, NONGL (SU) : -ACANTHOSIS / HYPERKERATOSIS,-MODERATE -ULCERATION,-SLIGHT -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 1921 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 172 ANIMAL NUMBER: C92557 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/27/99 STUDY DAY OF DEATH: 617 STUDY WEEK OF DEATH: 89 TERMINAL BODY WEIGHT: 486.8 GRAMS DATE AND TIME OF NECROPSY: 12/27/99 14:09 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) (FM), (SG), 1922 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 173 ANIMAL NUMBER: C92558 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/28/99 STUDY DAY OF DEATH: 557 STUDY WEEK OF DEATH: 80 TERMINAL BODY WEIGHT: 470.6 GRAMS DATE AND TIME OF NECROPSY: 10/28/99 8:02 PROSECTOR: JARED FISHEL RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >EYES; SQUINTED; PERI-ORBITAL; VERIFIED AT NECROPSY: NOT APPLICABLE >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; HUNCHED; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 12 X 7 X 6 MM, DARK RED, TAN, AND SOFT HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, PELVIC,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MINIMAL PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-MODERATE -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),SEVERE LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL HEART (HT) : -CARDIOMYOPATHY,-SLIGHT -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT 1923 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 174 ANIMAL NUMBER: C92558 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/28/99 STUDY DAY OF DEATH: 557 STUDY WEEK OF DEATH: 80 TERMINAL BODY WEIGHT: 470.6 GRAMS DATE AND TIME OF NECROPSY: 10/28/99 8:02 PROSECTOR: JARED FISHEL RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY STOMACH, GL (ST) : -EROSION/ULCERATION; MUCOSA: MULTIPLE TAN ERODED AREAS, PINPOINT AND UP TO 1 MM IN DIAMETER GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN GENERAL INFORMATION (XX) : >NOTE:>IDENTIFICATION TRANSPONDER FOUND WITHIN ADIPOSE TISSUE OF ABDOMINAL CAVITY STOMACH, GL (ST) : -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL JEJUNUM (JE) : >AUTOLYTIC PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 1924 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 175 ANIMAL NUMBER: C92558 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/28/99 STUDY DAY OF DEATH: 557 STUDY WEEK OF DEATH: 80 TERMINAL BODY WEIGHT: 470.6 GRAMS DATE AND TIME OF NECROPSY: 10/28/99 8:02 PROSECTOR: JARED FISHEL RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUm /s KULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 1925 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 176 ANIMAL NUMBER: C92559 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 10/22/99 STUDY DAY OF DEATH: 551 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 10/22/99 15:34 PROSECTOR: KEVIN BILLINGS RECORDER: KEVIN BILLINGS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); TAIL-PROXIMAL; WEEK 72; 25 X 20MM; VERIFIED AT NECROPSY: NOT OBSERVED >SKIN & PELAGE; SORE/SCAB; TAILPROXIMAL; VERIFIED AT NECROPSY: NOT OBSERVED >MASS(ES); VENTRAL-THORACIC; WEEK 79; 20 X 20MM; VERIFIED AT NECROPSY: NOT OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -LARGE; ALL LOBES: THICKENED -MOTTLED; ALL LOBES: RED, DARK RED AND TAN HISTOPATHOLOGY LIVER (LI) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT KIDNEY (KD) : -HYALINE DROPLETS, TUBULAR EPITHELIUM,MODERATE SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATELY SEVERE PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL -ATROPHY, ACINAR,-SLIGHT ADRENAL, CORTEX (AC) : -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL -HYPERTROPHY, CORTICAL CELL,-MINIMAL LN, MESENTERIC (MS) : -HEMORRHAGE,-PRESENT TESTIS (TE) : -ATROPHY/DEGENERATION,-MINIMAL MARROW, FEMUR (FM) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT 1926 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 177 ANIMAL NUMBER: C92559 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 10/22/99 STUDY DAY OF DEATH: 551 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 10/22/99 15:34 PROSECTOR: KEVIN BILLINGS RECORDER: KEVIN BILLINGS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY LUNG (LU) : -MOTTLED; ALL LOBES: DARK RED AND TAN SKIN (SK) : -MASS(ES); LEFT SACRAL REGION: SINGLE MASS, 25 MM IN DIAMETER; DARK RED, TAN AND FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED MARROW, STERNUM (SE) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATE LUNG (LU) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT -HEMORRHAGE,-PRESENT HEART (HT) : -CARDIOMYOPATHY,-MINIMAL PITUITARY (PI) : -HYPERPLASIA,-MINIMAL SKIN (SK) : -ACANTHOSIS/HYPERKERATOSIS,-SLIGHT -INFLAMMATION, CHRONIC-ACTIVE,MODERATELY SEVERE -BACTERIAL COLONIES,-PRESENT -SUPERFICIAL CRUSTING,-MODERATELY SEVERE -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL HEMATO NEOPLASIA (HN) : -M-SARCOMA, HISTIOCYTIC,-NEOPLASM PRESENT 1927 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 178 ANIMAL NUMBER: C92559 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 10/22/99 STUDY DAY OF DEATH: 551 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 10/22/99 15:34 PROSECTOR: KEVIN BILLINGS RECORDER: KEVIN BILLINGS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY LN, OTHER (LN) : -LARGE; HEPATIC: UP TO 10 X 6 X 6 MM (CAP 22) CAUSE OF DEATH (CD) : -NEOPLASM, HEMATOPOIETIC,-PRESENT LN, OTHER (LN) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUm /s KULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG), GENERAL COMMENT (GC) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), ADRENAL, MEDULLA (MA), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (JE), (EP) 1928 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 179 ANIMAL NUMBER: C92560 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/31/99 STUDY DAY OF DEATH: 560 STUDY WEEK OF DEATH: 80 TERMINAL BODY WEIGHT: 588.2 GRAMS DATE AND TIME OF NECROPSY: 10/31/99 14:18 PROSECTOR: TROY ELLENBECKER RECORDER: TROY ELLENBECKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: TROY ELLENBECKER *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); TAIL-PROXIMAL; WEEK 79; VERIFIED AT NECROPSY: OBSERVED >RESPIRATION; IRREGULAR; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; YELLOW HAIR COAT; VERIFIED AT NECROPSY: NOT OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) KIDNEY (KD) : -ROUGH SURFACE, DIFFUSE; BILATERAL HISTOPATHOLOGY BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MODERATELY SEVERE SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE PANCREAS (PA) : -B-ADENOMA, ISLET CELL,-NEOPLASM PRESENT ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-MINIMAL MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,-MODERATE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATELY SEVERE HEART (HT) : -CARDIOMYOPATHY,-MINIMAL 1929 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 180 ANIMAL NUMBER: C92560 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/31/99 STUDY DAY OF DEATH: 560 STUDY WEEK OF DEATH: 80 TERMINAL BODY WEIGHT: 588.2 GRAMS DATE AND TIME OF NECROPSY: 10/31/99 14:18 PROSECTOR: TROY ELLENBECKER RECORDER: TROY ELLENBECKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: TROY ELLENBECKER LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 10 X 4 X 4 MM, RED, DARK RED, TAN AND SOFT TAIL (TI) : -RAISED AREA(S); NEAR BASE OF TAIL: SINGLE SOFT AND RAISED AREA, 15 X 10 X 5 MM; COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DRY RED MATERIAL THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT TAIL (TI) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE -CYST, EPIDERMAL INCLUSION,-PRESENT 1930 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 181 ANIMAL NUMBER: C92560 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/31/99 STUDY DAY OF DEATH: 560 STUDY WEEK OF DEATH: 80 TERMINAL BODY WEIGHT: 588.2 GRAMS DATE AND TIME OF NECROPSY: 10/31/99 14:18 PROSECTOR: TROY ELLENBECKER RECORDER: TROY ELLENBECKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: TROY ELLENBECKER THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 1931 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 182 ANIMAL NUMBER: C92561 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/29/99 STUDY DAY OF DEATH: 436 STUDY WEEK OF DEATH: 63 TERMINAL BODY WEIGHT: 624.5 GRAMS DATE AND TIME OF NECROPSY: 06/29/99 14:35 PROSECTOR: JERALD W. JOHNSON RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >BEHAVIOR; RECUMBENT; LATERAL; VERIFIED AT NECROPSY: NOT APPLICABLE >RESPIRATION; AUDIBLE; VERIFIED AT NECROPSY: NOT APPLICABLE >RESPIRATION; IRREGULAR; VERIFIED AT NECROPSY: NOT APPLICABLE >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) HISTOPATHOLOGY BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT -HEMORRHAGE,-MINIMAL LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -NECROSIS, INDIVIDUAL HEPATOCYTE,MINIMAL -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL -ANGIECTASIS,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SLIGHT -PIGMENT, TUBULAR,-MINIMAL -HYALINE DROPLETS, TUBULAR EPITHELIUM,MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-SLIGHT 1932 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 183 ANIMAL NUMBER: C92561 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/29/99 STUDY DAY OF DEATH: 436 STUDY WEEK OF DEATH: 63 TERMINAL BODY WEIGHT: 624.5 GRAMS DATE AND TIME OF NECROPSY: 06/29/99 14:35 PROSECTOR: JERALD W. JOHNSON RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 8 MM IN DIAMETER, DARK RED, GRAY AND SOFT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN THYROID (TY) : -DILATATION / CYST, FOLLICULAR,-PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT -ANGIECTASIS,-PRESENT RECTUM (RE) : -PARASITISM,-PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 1933 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 184 ANIMAL NUMBER: C92561 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/29/99 STUDY DAY OF DEATH: 436 STUDY WEEK OF DEATH: 63 TERMINAL BODY WEIGHT: 624.5 GRAMS DATE AND TIME OF NECROPSY: 06/29/99 14:35 PROSECTOR: JERALD W. JOHNSON RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 1934 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 185 ANIMAL NUMBER: C92562 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 667.7 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 8:48 PROSECTOR: GREGORY CODDINGTON RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT' (%) LF ADRENAL (AD0) RT ADRENAL (AD1) BRAIN (BR) LF KIDNEY (KD0) RT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LF TESTIS (TE0) RT TESTIS (TE1) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) .0217 .0222 2.3159 2.1698 2.1881 19.3134 1.9553 .9195 1.6813 1.6647 .0204 .0190 .0032 % .0033 % .3468 % .3250 % .3277 % 2.8925 % .2928 % .1377 % .2518 % .2493 % .0031 % .0028 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0094 .0096 1.0000 .9369 .9448 8.3395 .8443 .3970 .7260 .7188 .0088 .0082 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL -VACUOLATION, HEPATOCELLULAR,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MINIMAL 1935 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 186 ANIMAL NUMBER: C92562 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 667.7 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 8:48 PROSECTOR: GREGORY CODDINGTON RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MINIMAL PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL ADRENAL, CORTEX (AC) : -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL MARROW, FEMUR (FM) : -HYPOCELLULAR, MARROW,-SLIGHT LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL PITUITARY (PI) : -HYPERPLASIA,-MINIMAL PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 1936 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 187 ANIMAL NUMBER: C92562 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 667.7 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 8:48 PROSECTOR: GREGORY CODDINGTON RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) (CE), 1937 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 188 ANIMAL NUMBER: C92563 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/24/99 STUDY DAY OF DEATH: 584 STUDY WEEK OF DEATH: 84 TERMINAL BODY WEIGHT: 493.7 GRAMS DATE AND TIME OF NECROPSY: 11/24/99 8:04 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >EXCRETION; NO FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; THIN; VERIFIED AT NECROPSY: NOT OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) HISTOPATHOLOGY BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL -INFILTRATION, FAT, INTERSTITIAL,SLIGHT ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MINIMAL BONE, STERNUM (SB) : -HYPERPLASIA, CARTILAGINOUS,-SLIGHT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATE HEART (HT) : -CARDIOMYOPATHY,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT 1938 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 189 ANIMAL NUMBER: C92563 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/24/99 STUDY DAY OF DEATH: 584 STUDY WEEK OF DEATH: 84 TERMINAL BODY WEIGHT: 493.7 GRAMS DATE AND TIME OF NECROPSY: 11/24/99 8:04 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 10 X 8 X 7 MM, DARK RED, TAN AND SOFT MAMMARY, MALE (MM) : -CYST(S); BILATERAL PECTORAL REGIONS: MULTIPLE TAN AND CREAMY SEMIFLUID FILLED CYSTS, UP TO 4 MM IN DIAMETER (CAP 22) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN STOMACH, NONGL (SU) : -ACANTHOSIS / HYPERKERATOSIS,-MODERATE -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,MODERATELY SEVERE CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT MAMMARY, MALE (MM) : -GALACTOCELE,-PRESENT -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT 1939 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 190 ANIMAL NUMBER: C92563 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/24/99 STUDY DAY OF DEATH: 584 STUDY WEEK OF DEATH: 84 TERMINAL BODY WEIGHT: 493.7 GRAMS DATE AND TIME OF NECROPSY: 11/24/99 8:04 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUm /s KULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) (SN), 1940 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 191 ANIMAL NUMBER: C92564 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/19/99 STUDY DAY OF DEATH: 487 STUDY WEEK OF DEATH: 70 TERMINAL BODY WEIGHT: 681.9 GRAMS DATE AND TIME OF NECROPSY: 08/19/99 7:55 PROSECTOR: TONY HANFELD RECORDER: KEVIN BILLINGS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >EXCRETION; NONFORMED FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; HEAD TILT; RIGHT; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; THIN; VERIFIED AT NECROPSY: NOT OBSERVED OBSERVATIONS NECROPSY PITUITARY (PI) : -LARGE; 5 X 5 X 2 ; APPEARS SWOLLEN HISTOPATHOLOGY BRAIN (BR) : -M-ASTROCYTOMA,-NEOPLASM PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MODERATE SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,SLIGHT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATE HEART (HT) : -CARDIOMYOPATHY,-MINIMAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT 1941 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 192 ANIMAL NUMBER: C92564 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/19/99 STUDY DAY OF DEATH: 487 STUDY WEEK OF DEATH: 70 TERMINAL BODY WEIGHT: 681.9 GRAMS DATE AND TIME OF NECROPSY: 08/19/99 7:55 PROSECTOR: TONY HANFELD RECORDER: KEVIN BILLINGS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -RAISED AREA(S); RIGHT POSTERIOR ASPECT: SINGLE TAN AREA, 1 MM IN DIAMETER STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA, WITHIN THICKENED AREA: MULTIPLE DARK BROWN ERODED AREAS, UP TO 8 X 2 MM -THICKENED WALL; NEAR ESOPHAGEAL GROOVE: SINGLE AREA, 15 X 9 MM GENERAL COMMENT (GC) : -BLOOD FILM TAKEN STOMACH, GL (ST) : -INFLAMMATION, ACUTE,-MINIMAL -HYPERPLASIA, EPITHELIAL, GLANDULAR,SLIGHT STOMACH, NONGL (SU) : -ACANTHOSIS / HYPERKERATOSIS,-MODERATE -ULCERATION,-MINIMAL -INFLAMMATION, CHRONIC-ACTIVE,MODERATELY SEVERE CAUSE OF DEATH (CD) : -NEOPLASM, BRAIN,-PRESENT 1942 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 193 ANIMAL NUMBER: C92564 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/19/99 STUDY DAY OF DEATH: 487 STUDY WEEK OF DEATH: 70 TERMINAL BODY WEIGHT: 681.9 GRAMS DATE AND TIME OF NECROPSY: 08/19/99 7:55 PROSECTOR: TONY HANFELD RECORDER: KEVIN BILLINGS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAr /i LIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 1943 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 194 ANIMAL NUMBER: C92565 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 01/16/00 STUDY DAY OF DEATH: 637 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 01/16/00 8:28 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >RESPIRATION; LABORED; VERIFIED AT NECROPSY: NOT APPLICABLE >DISCHARGE; NASAL; RED IN COLOR; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; LIMITED USE; PAWS-HIND; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY HISTOPATHOLOGY EYE (EY) : -INFLAMMATION, ACUTE,-SLIGHT LIVER (LI) : -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-SLIGHT -HYPERPLASIA, BILE DUCT,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL -B-ADENOMA, ISLET CELL,-NEOPLASM PRESENT ADRENAL, CORTEX (AC) : -HYPERTROPHY, CORTICAL CELL,-MINIMAL TESTIS (TE) : -ATROPHY/DEGENERATION,-MINIMAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATELY SEVERE 1944 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 195 ANIMAL NUMBER: C92565 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 01/16/00 STUDY DAY OF DEATH: 637 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 01/16/00 8:28 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY STOMACH, GL (ST) : -THICKENED WALL; DIFFUSE HEART (HT) : -CARDIOMYOPATHY,-SLIGHT MUSCLE, SKELETAL (SM) : -ATROPHY,-MODERATELY SEVERE NERVE, SCIATIC (SN) : -DEGENERATION,-MINIMAL PITUITARY (PI) : -HYPERPLASIA,-SLIGHT STOMACH, GL (ST) : -HYPERPLASIA, EPITHELIAL, GLANDULAR,SLIGHT STOMACH, NONGL (SU) : -ACANTHOSIS / HYPERKERATOSIS,-MINIMAL -INFLAMMATION, SUBACUTE,-MINIMAL DUODENUM (DU) : -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL -EROSION,-MINIMAL ILEUM (IL) : >AUTOLYTIC CECUM (CE) : >AUTOLYTIC URINARY BLADDER (UB) : -PROTEINACEOUS MATERIAL, LUMINAL,PRESENT EPIDIDYMIDES (EP) : -IMMATURE SPERM FORMS,-MINIMAL CAUSE OF DEATH (CD) : -UNDETERMINED MICROSCOPICALLY,-PRESENT 1945 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 196 ANIMAL NUMBER: C92565 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 01/16/00 STUDY DAY OF DEATH: 637 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 01/16/00 8:28 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -DISCHARGE-OCULAR; BILATERAL: DARK RED AND MOIST -STAINS-PERINEUM/PERIANAL; YELLOW AND MOIST -DISCHARGE-NASAL; DARK RED AND MOIST -ANIMAL OBESE 1946 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 197 ANIMAL NUMBER: C92565 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 01/16/00 STUDY DAY OF DEATH: 637 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 01/16/00 8:28 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: BRAIN (BR), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), JEJUNUM (JE), COLON (CO), RECTUM (RE), SKIN (SK), PROSTATE (PR), SEMINAL VESICLES (SV), HEMATO NEOPLASIA (HN) 1947 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 198 ANIMAL NUMBER: C92566 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/21/98 STUDY DAY OF DEATH: 155 STUDY WEEK OF DEATH: 23 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 09/21/98 10:53 PROSECTOR: JAMES T. CALLEN RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -TEETH MISSING/BROKEN/WORN; UPPER INCISORS: MISSING BILATERAL HISTOPATHOLOGY EYE (EY) : -DYSTROPHY, CORNEAL IN EARLY STAGES, CONSISTS PRIMARILY OF FOCAL MINERALIZATION,-MINIMAL BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT -M-OLIGODENDROGLIOMA,-NEOPLASM PRESENT LIVER (LI) : -VACUOLATION, HEPATOCELLULAR, MIDZONAL / CENTRILOBULAR,-MODERATE KIDNEY (KD) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -REGENERATION, TUBULAR EPITHELIUM,MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT LUNG (LU) : -CONGESTION,-PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, BRAIN,-PRESENT 1948 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 199 ANIMAL NUMBER: C92566 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/21/98 STUDY DAY OF DEATH: 155 STUDY WEEK OF DEATH: 23 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 09/21/98 10:53 PROSECTOR: JAMES T. CALLEN RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -DISCHARGE-OCULAR; LEFT: DRY RED MATERIAL 1949 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 200 ANIMAL NUMBER: C92566 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/21/98 STUDY DAY OF DEATH: 155 STUDY WEEK OF DEATH: 23 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 09/21/98 10:53 PROSECTOR: JAMES T. CALLEN RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 1950 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 201 ANIMAL NUMBER: C92567 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 01/12/00 STUDY DAY OF DEATH: 633 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 01/12/00 8:22 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) KIDNEY (KD) : -LARGE PELVIS(ES); BILATERAL HISTOPATHOLOGY BRAIN (BR) : >UNREMARKABLE LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -NECROSIS, INDIVIDUAL HEPATOCYTE,MINIMAL -DEGENERATION, CYSTIC,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE PANCREAS (PA) : -INFILTRATION, FAT, INTERSTITIAL,SLIGHT MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,-SLIGHT MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,-SLIGHT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATE 1951 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 202 ANIMAL NUMBER: C92567 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 01/12/00 STUDY DAY OF DEATH: 633 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 01/12/00 8:22 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY MUSCLE, SKELETAL (SM) : -MASS(ES); LEFT BICEPS FEMORIS: SINGLE MASS, 40 X 20 X 20 MM, DARK RED, TAN, LOBULATED, AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (POPLITEAL) NOT IDENTIFIED NERVE, SCIATIC (SN) : >NOTE:>RIGHT SCIATIC NERVE COLLECTED DUE TO SM MASS ON LEFT THYROID (TY) : -LARGE; RIGHT: 9 X 7 X 7 MM (LEFT IS 8 X 5 X 5 MM) PITUITARY (PI) : -MASS(ES); 8 X 7 X 5 MM, DARK RED, TAN, AND SOFT HEART (HT) : -CARDIOMYOPATHY,-SLIGHT MUSCLE, SKELETAL (SM) : -M-SARCOMA, UNDIFFERENTIATED,-NEOPLASM PRESENT THYROID (TY) : >UNREMARKABLE PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT DUODENUM (DU) : >AUTOLYTIC JEJUNUM (JE) : >AUTOLYTIC ILEUM (IL) : >AUTOLYTIC CECUM (CE) : >AUTOLYTIC COLON (CO) : >AUTOLYTIC RECTUM (RE) : >AUTOLYTIC 1952 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 203 ANIMAL NUMBER: C92567 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 01/12/00 STUDY DAY OF DEATH: 633 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 01/12/00 8:22 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -ANIMAL OBESE PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE CAUSE OF DEATH (CD) : -NEOPLASM, SKIN/MUSCLE/SUBCUTIS,PRESENT 1953 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 204 ANIMAL NUMBER: C92567 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 01/12/00 STUDY DAY OF DEATH: 633 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 01/12/00 8:22 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), SKIN URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) (SK), 1954 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 205 ANIMAL NUMBER: C92568 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/20/00 STUDY DAY OF DEATH: 701 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/20/00 13:35 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >WART LIKE LESION(S); DORSAL; WEEK 95; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; DORSAL; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY KIDNEY (KD) : -CYST(S); RIGHT ON CORTICAL SURFACE: SINGLE CLEAR FLUID FILLED CYST, 1 MM IN DIAMETER -LARGE; BILATERAL: EACH IS 30 X 20 X 12 MM ADRENAL, CORTEX (AC) : -LARGE; LEFT:9 X 6 X 6 MM (RIGHT IS 5 X 4 X 2 MM) -MOTTLED; BILATERAL: RED, DARK RED AND TAN HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SEVERE -DILATATION, PELVIC,-SLIGHT -MINERALIZATION, PELVIC,-MINIMAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : -B-ADENOMA,-NEOPLASM PRESENT ADRENAL, MEDULLA (MA) : -B-PHEOCHROMOCYTOMA,-NEOPLASM PRESENT -M-MALIGNANT PHEOCHROMOCYTOMA,-NEOPLASM PRESENT 1955 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 206 ANIMAL NUMBER: C92568 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/20/00 STUDY DAY OF DEATH: 701 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/20/00 13:35 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY SKIN (SK) : -CRUSTED AREA(S); DORSAL THORACIC REGION: SINGLE DARK RED, DRY SLIGHTLY RAISED AREA, 10 X 6 X 4 MM (CAP 22) TESTIS (TE) : -ATROPHY/DEGENERATION,-MINIMAL -INFLAMMATION, VASCULAR,-MODERATELY SEVERE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MINIMAL HEART (HT) : -CARDIOMYOPATHY,-MODERATE THYROID (TY) : -DILATATION / CYST, FOLLICULAR,-PRESENT PARATHYROID (PT) : -HYPERPLASIA,-SLIGHT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT SKIN (SK) : -M-CARCINOMA, SQUAMOUS CELL,-NEOPLASM PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT EPIDIDYMIDES (EP) : -INFLAMMATION, VASCULAR,-SLIGHT CAUSE OF DEATH (CD) : -NEPHROPATHY,-PRESENT 1956 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 207 ANIMAL NUMBER: C92568 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/20/00 STUDY DAY OF DEATH: 701 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/20/00 13:35 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG), GENERAL COMMENT (GC) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), SEMINAL VESICLES (SV), HEMATO NEOPLASIA (HN) 1957 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 208 ANIMAL NUMBER: C92569 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 601.2 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 9:59 PROSECTOR: GREGORY CODDINGTON RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); AXILLARY REGION-LEFT; WEEK 93; 10 X 10MM; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY HISTOPATHOLOGY EYE (EY) : -DYSTROPHY, CORNEAL IN EARLY STAGES, CONSISTS PRIMARILY OF FOCAL MINERALIZATION,-MINIMAL LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-SLIGHT -HYPERPLASIA, BILE DUCT,-SLIGHT -FIBROSIS, PERIBILIARY,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MODERATE SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL -ATROPHY, ACINAR,-MINIMAL -INFILTRATION, FAT, INTERSTITIAL,MODERATE ADRENAL, CORTEX (AC) : -HYPERTROPHY, CORTICAL CELL,-MINIMAL 1958 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 209 ANIMAL NUMBER: C92569 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 601.2 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 9:59 PROSECTOR: GREGORY CODDINGTON RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY MAMMARY, MALE (MM) : -MASS(ES); LEFT AXILLARY REGION: SINGLE MASS, 20 X 15 X 10 MM, RED, TAN, GREY AND FIRM; SAME ON CUT SURFACE; (CAP 22); REGIONAL LN (AXILLARY) NORMAL (CAP 23) -MASS #2; LEFT AXILLARY REGION, CRANIAL TO MM MASS #1: SINGLE MASS, 25 X 15 X 12 MM, TAN, LOBULATED AND FIRM; SAME ON CUT SURFACE; (CAP 24); REGIONAL LN (AXILLARY) NORMAL (CAP 23) ADRENAL, CORTEX (AC) : -PIGMENT,-SLIGHT LN, MESENTERIC (MS) : -HEMORRHAGE,-PRESENT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),SLIGHT HEART (HT) : -CARDIOMYOPATHY,-MINIMAL PITUITARY (PI) : -HYPERPLASIA,-MINIMAL HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT MAMMARY, MALE (MM) : -INFLAMMATION, CHRONIC-ACTIVE,MODERATELY SEVERE -M-CARCINOMA,- 2 NEOPLASMS PRESENT 1959 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 210 ANIMAL NUMBER: C92569 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 601.2 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 9:59 PROSECTOR: GREGORY CODDINGTON RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY CAVITY, ABDOM (PC) : -MASS(ES); ATTACHED TO ADIPOSE TISSUE, NEAR STOMACH: SINGLE MASS, 20 X 12 X 10 MM, YELLOW, TAN AND FIRM; (CAP 25) REGIONAL LN (PANCREATIC) NOT IDENTIFIED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN CAVITY, ABDOM (PC) : -INFLAMMATION / NECROSIS, FAT,MODERATELY SEVERE -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE 1960 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 211 ANIMAL NUMBER: C92569 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 601.2 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 9:59 PROSECTOR: GREGORY CODDINGTON RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: BRAIN (BR), ADRENAL, MEDULLA (MA), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN), LN, AXILLARY (AX) 1961 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 212 ANIMAL NUMBER: C92570 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 648.0 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 9:52 PROSECTOR: NANCY DIEDRICH RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT' (%) LF ADRENAL (AD0) RT ADRENAL (AD1) BRAIN (BR) LF KIDNEY (KD0) RT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LF TESTIS (TE0) RT TESTIS (TE1) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) .0288 .0271 2.4190 2.6246 2.5830 17.2061 2.5087 .9586 1.9006 1.9018 .0274 .0279 .0044 % .0042 % .3733 % .4050 % .3986 % 2.6553 % .3871 % .1479 % .2933 % .2935 % .0042 % .0043 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0119 .0112 1.0000 1.0850 1.0678 7.1129 1.0371 .3963 .7857 .7862 .0113 .0115 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-MINIMAL PANCREAS (PA) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL -ATROPHY, ACINAR,-MODERATE 1962 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 213 ANIMAL NUMBER: C92570 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 648.0 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 9:52 PROSECTOR: NANCY DIEDRICH RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN ADRENAL, CORTEX (AC) : -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL MARROW, FEMUR (FM) : -HYPOCELLULAR, MARROW,-SLIGHT HEART (HT) : -CARDIOMYOPATHY,-MINIMAL STOMACH, GL (ST) : -INFLAMMATION, ACUTE,-MINIMAL STOMACH, NONGL (SU) : -ACANTHOSIS / HYPERKERATOSIS,-MINIMAL URINARY BLADDER (UB) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 1963 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 214 ANIMAL NUMBER: C92570 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 648.0 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 9:52 PROSECTOR: NANCY DIEDRICH RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 1964 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 215 ANIMAL NUMBER: C92571 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/21/00 STUDY DAY OF DEATH: 702 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: 645.6 GRAMS DATE AND TIME OF NECROPSY: 03/21/00 12:32 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; HEAD TILT; LEFT; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; ATAXIC; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-SLIGHT -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-MODERATE -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC.-MINIMAL -DEGENERATION, CYSTIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-SLIGHT -FIBROSIS, PERIBILIARY,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SLIGHT -HYPERPLASIA, TRANSITIONAL CELL,MODERATE -INFLAMMATION, SUPPURATIVE, PELVIC,MODERATE SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SLIGHT ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-SLIGHT 1965 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 216 ANIMAL NUMBER: C92571 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/21/00 STUDY DAY OF DEATH: 702 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: 645.6 GRAMS DATE AND TIME OF NECROPSY: 03/21/00 12:32 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY TESTIS (TE) : -SMALL; LEFT: 20 X 12 X 3 MM (RIGHT IS 23 X 14 X 8 MM); LEFT IS SOFT PITUITARY (PI) : -MASS(ES); 12 X 10 X 6 MM, RED, DARK RED AND SOFT URINARY BLADDER (UB) : -SEMIFLUID MATERIAL; RED SEMIFLUID -THICKENED WALL; DIFFUSE GENERAL COMMENT (GC) : -BLOOD FILM TAKEN ADRENAL, MEDULLA (MA) : -UNILATERALLY EXAMINED,-PRESENT -HYPERPLASIA, MEDULLARY,-MINIMAL TESTIS (TE) : -ATROPHY/DEGENERATION,-SEVERE >NOTE:>UNILATERAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATELY SEVERE HEART (HT) : -CARDIOMYOPATHY,-MINIMAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT URINARY BLADDER (UB) : -HEMORRHAGE,-MINIMAL -HYPERPLASIA, TRANSITIONAL CELL,-SLIGHT -INFLAMMATION, CHRONIC-ACTIVE,MODERATELY SEVERE PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,MODERATELY SEVERE CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 1966 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 217 ANIMAL NUMBER: C92571 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/21/00 STUDY DAY OF DEATH: 702 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: 645.6 GRAMS DATE AND TIME OF NECROPSY: 03/21/00 12:32 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -MALOCCLUSION; BILATERAL, UPPER INCISORS THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUm /s KULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 1967 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 218 ANIMAL NUMBER: C92572 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/21/00 STUDY DAY OF DEATH: 702 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/21/00 15:56 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; THIN; VERIFIED AT NECROPSY: NOT OBSERVED OBSERVATIONS NECROPSY KIDNEY (KD) : -ROUGH SURFACE, DIFFUSE; BILATERAL -MOTTLED; BILATERAL: RED AND TAN HISTOPATHOLOGY EYE (EY) : -DYSTROPHY, CORNEAL IN EARLY STAGES, CONSISTS PRIMARILY OF FOCAL MINERALIZATION,-MINIMAL BRAIN (BR) : -MINERALIZATION,-MINIMAL LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SEVERE -MINERALIZATION, PELVIC,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-MODERATE -INFILTRATION, FAT, INTERSTITIAL,MODERATE ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-MODERATELY SEVERE 1968 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 219 ANIMAL NUMBER: C92572 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/21/00 STUDY DAY OF DEATH: 702 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/21/00 15:56 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY ADRENAL, MEDULLA (MA) : -UNILATERALLY EXAMINED,-PRESENT LN, MESENTERIC (MS) : -MINERALIZATION, VASCULAR,-MODERATE TESTIS (TE) : -ATROPHY/DEGENERATION,-SLIGHT -INFLAMMATION, VASCULAR,-MODERATE -HYPERPLASIA, INTERSTITIAL CELL (LEYDIG CELL),-SLIGHT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),SLIGHT LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL HEART (HT) : -CARDIOMYOPATHY,-MODERATE -MINERALIZATION, VASCULAR,-MODERATE -MINERALIZATION,-MODERATE PARATHYROID (PT) : >SECTION EXAMINED; TISSUE NOT PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : -MINERALIZATION, GLANDULAR MUCOSA,SLIGHT -MINERALIZATION, VASCULAR,-SLIGHT CECUM (CE) : >AUTOLYTIC 1969 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 220 ANIMAL NUMBER: C92572 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/21/00 STUDY DAY OF DEATH: 702 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/21/00 15:56 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -DISCHARGE-ORAL; BROWN MATERIAL COLON (CO) : >AUTOLYTIC URINARY BLADDER (UB) : -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT CAUSE OF DEATH (CD) : -NEPHROPATHY,-PRESENT 1970 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 221 ANIMAL NUMBER: C92572 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/21/00 STUDY DAY OF DEATH: 702 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/21/00 15:56 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAr /i LIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), RECTUM (RE), SKIN (SK), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 1971 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 222 ANIMAL NUMBER: C92573 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/24/99 STUDY DAY OF DEATH: 584 STUDY WEEK OF DEATH: 84 TERMINAL BODY WEIGHT: 530.3 GRAMS DATE AND TIME OF NECROPSY: 11/24/99 7:41 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MISSING; TEETH; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: NOT OBSERVED >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >EYES; RED DISCHARGE; EYES; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) HISTOPATHOLOGY BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -VACUOLATION, HEPATOCELLULAR,-SLIGHT -GLYCOGEN, HEPATOCELLULAR, INCREASED,MODERATELY SEVERE KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-MODERATE -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL -INFILTRATION, FAT, INTERSTITIAL,MODERATE ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC / HEMORRHAGIC,SLIGHT TESTIS (TE) : -ATROPHY/DEGENERATION,-SLIGHT >NOTE:>UNILATERAL 1972 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 223 ANIMAL NUMBER: C92573 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/24/99 STUDY DAY OF DEATH: 584 STUDY WEEK OF DEATH: 84 TERMINAL BODY WEIGHT: 530.3 GRAMS DATE AND TIME OF NECROPSY: 11/24/99 7:41 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY THYMUS (TH) : -MISSING/NOT IDENTIFIED; NOT POSITIVELY IDENTIFIED; POSSIBLE REMNANTS COLLECTED PITUITARY (PI) : -MASS(ES); 9 X 7 X 7 MM, DARK RED, RED, TAN AND SOFT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; RIGHT UPPER INCISOR -TEETH MISSING/BROKEN/WORN; LEFT UPPER INCISOR: MISSING -DISCHARGE-OCULAR; BILATERAL: DARK RED AND DRY THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),SEVERE HEART (HT) : -CARDIOMYOPATHY,-MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-SLIGHT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 1973 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 224 ANIMAL NUMBER: C92573 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/24/99 STUDY DAY OF DEATH: 584 STUDY WEEK OF DEATH: 84 TERMINAL BODY WEIGHT: 530.3 GRAMS DATE AND TIME OF NECROPSY: 11/24/99 7:41 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUm /s KULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 1974 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 225 ANIMAL NUMBER: C92574 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 330.1 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 9:13 PROSECTOR: JODI THIEREN RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN STATUS LF ADRENAL (AD0) RT ADRENAL (AD1) BRAIN (BR) LF KIDNEY (KD0) RT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LF TESTIS (TE0) RT TESTIS (TE1) LF THYROID/PARA RT THYROID/PARA (TT0) (TT1) ------------------------------------ 12.0928 ------------------------------------------- ------------------------------------ 3.6630 % ------------------------------------------- ------------------------------------------------------------------------------------- NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN WEIGHT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS 1975 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 226 ANIMAL NUMBER: C92574 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 330.1 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 9:13 PROSECTOR: JODI THIEREN RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) 1976 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 227 ANIMAL NUMBER: C92575 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 288.1 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 10:32 PROSECTOR: LYNN HORNER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN STATUS LF ADRENAL (AD0) RT ADRENAL (AD1) BRAIN (BR) LF KIDNEY (KD0) RT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LF TESTIS (TE0) RT TESTIS (TE1) LF THYROID/PARA RT THYROID/PARA (TT0) (TT1) ------------------------------------ 10.4038 ------------------------------------------- ------------------------------------ 3.6115 % ------------------------------------------- ------------------------------------------------------------------------------------- NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN WEIGHT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS 1977 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 228 ANIMAL NUMBER: C92575 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 288.1 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 10:32 PROSECTOR: LYNN HORNER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) 1978 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 229 ANIMAL NUMBER: C92576 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/21/00 STUDY DAY OF DEATH: 673 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 792.6 GRAMS DATE AND TIME OF NECROPSY: 02/21/00 14:34 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); DORSAL; WEEK 73; 30 X 40MM; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; DORSAL; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; LIMITED USE; LIMBS-HIND; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) HISTOPATHOLOGY BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -VACUOLATION, HEPATOCELLULAR, MIDZONAL / CENTRILOBULAR,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MODERATE -MINERALIZATION, PELVIC,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SLIGHT PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-MODERATE -INFILTRATION, FAT, INTERSTITIAL,SLIGHT ADRENAL, CORTEX (AC) : -PIGMENT,-SLIGHT ADRENAL, MEDULLA (MA) : -B-PHEOCHROMOCYTOMA,-NEOPLASM PRESENT BONE, FEMUR (FE) : -HYPERPLASIA, BONE,-SLIGHT 1979 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 230 ANIMAL NUMBER: C92576 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/21/00 STUDY DAY OF DEATH: 673 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 792.6 GRAMS DATE AND TIME OF NECROPSY: 02/21/00 14:34 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY HEART (HT) : -LIGHT FOCUS(I)/AREA(S); EPICARDIUM OF BOTH VENTRICLES: MULTIPLE TAN, INDISTINCT AREAS PITUITARY (PI) : -MASS(ES); 8 X 5 X 5 MM, RED, DARK RED, AND SOFT SKIN (SK) : -CRUSTED AREA(S); DORSAL THORACIC REGION: SINGLE BLACK CRUSTED AREA, 30 X 20 MM; COLLECTED; POSSIBLY CONSISTENT WITH MASS NOTED IN CLINICAL OBSERVATIONS GENERAL COMMENT (GC) : -BLOOD FILM TAKEN THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATELY SEVERE HEART (HT) : -CARDIOMYOPATHY,-SLIGHT THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT SKIN (SK) : -INFLAMMATION, CHRONIC-ACTIVE,MODERATELY SEVERE -ULCERATION,-SLIGHT -B-KERATOACANTHOMA,-NEOPLASM PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 1980 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 231 ANIMAL NUMBER: C92576 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/21/00 STUDY DAY OF DEATH: 673 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 792.6 GRAMS DATE AND TIME OF NECROPSY: 02/21/00 14:34 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LN, MESENTERIC (MS), TESTIS (TE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 1981 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 232 ANIMAL NUMBER: C92577 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 895.6 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 9:52 PROSECTOR: JOHN S. HALFORD RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); DORSAL; WEEK 69; 30 X 30MM; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; DORSAL; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); AXILLARY REGION-LEFT; WEEK 101; 30 X 40MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); HEAD-MAXILLARY-LEFT; WEEK 105; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY SKIN (SK) : -MASS(ES); ATTACHED TO LOWER LEFT LIP: SINGLE MASS, 15 X 13 X 8 MM, RED, DARK RED, GRAY, AND SOFT; ON SECTION EXUDES A DARK BROWN GELATINOUS FLUID (CAP 22); REGIONAL LN (MANDIBULAR) NORMAL (CAP 23) HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MODERATELY SEVERE -CYST,-PRESENT PANCREAS (PA) : -ATROPHY, ACINAR,-MINIMAL -B-ADENOMA, ISLET CELL,-NEOPLASM PRESENT TESTIS (TE) : -INFLAMMATION, VASCULAR,-SLIGHT THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT SKIN (SK) : -INFLAMMATION, CHRONIC-ACTIVE,MODERATELY SEVERE -B-KERATOACANTHOMA,-NEOPLASM PRESENT -B-FIBROMA,-NEOPLASM PRESENT -FIBROSIS,-MODERATE >NOTE:>MASS #2 IS THE KERATOACANTHOMA THE FIBROMA WAS DESCRIBED GROSSLY UNDER MAMMARY, MALE 1982 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 233 ANIMAL NUMBER: C92577 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 895.6 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 9:52 PROSECTOR: JOHN S. HALFORD RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY SKIN (SK) : -MASS #2; RIGHT LUMBAR REGION: SINGLE MASS, 32 X 20 X 19 MM, RED, GRAY, TAN, CAULIFLOWER-LIKE, AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (INGUINAL) NORMAL (CAP 26); OVERLYING SKIN HAS A SINGLE DRY, TAN CRUSTED AREA, 10 X 10 X 5 MM; COLLECTED INTACT WITH MASS MAMMARY, MALE (MM) : -MASS(ES); LEFT AXILLARY REGION: SINGLE MASS, 53 X 45 X 18 MM, RED, GRAY, TAN, AND SOFT; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (AXILLARY) NORMAL (CAP 24) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -ANIMAL OBESE URINARY BLADDER (UB) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, AXILLARY (AX) : -HYPERPLASIA, LYMPHOPLASMACYTIC,MODERATE LN, MANDIBULAR (MN) : -HYPERPLASIA, LYMPHOPLASMACYTIC,MODERATE MAMMARY, MALE (MM) : >NOT REQUIRED TO BE EXAMINED FOR ANIMAL >NOTE:>SEE SKIN FOR DIAGNOSIS OF MASS THIS MASS IS THE FIBROMA 1983 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 234 ANIMAL NUMBER: C92577 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 895.6 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 9:52 PROSECTOR: JOHN S. HALFORD RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PARATHYROID (PT), LN, INGUINAL (IN) 1984 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 235 ANIMAL NUMBER: C92578 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/08/99 STUDY DAY OF DEATH: 598 STUDY WEEK OF DEATH: 86 TERMINAL BODY WEIGHT: 707.7 GRAMS DATE AND TIME OF NECROPSY: 12/08/99 12:52 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; THIN; VERIFIED AT NECROPSY: NOT OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; ATAXIC; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; PALE; ENTIRE BODY; VERIFIED AT NECROPSY: NOT APPLICABLE >EYES; RED DISCHARGE; EYE-LEFT; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -LIGHT FOCUS(I)/AREA(S); RIGHT MEDIAN LOBE, AT JUNCTION OF LEFT MEDIAN LOBE: SINGLE TAN AREA, 4 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION -RED FOCUS(I)/AREA(S); LEFT LATERAL LOBE: MULTIPLE DARK RED AREAS, UP TO 3 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION HISTOPATHOLOGY BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-SLIGHT -DEGENERATION, CYSTIC,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MINIMAL PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-MODERATE -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL -INFILTRATION, FAT, INTERSTITIAL,MINIMAL 1985 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 236 ANIMAL NUMBER: C92578 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/08/99 STUDY DAY OF DEATH: 598 STUDY WEEK OF DEATH: 86 TERMINAL BODY WEIGHT: 707.7 GRAMS DATE AND TIME OF NECROPSY: 12/08/99 12:52 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 12 X 6 X 5 MM, DARK RED, TAN, SOFT AND LOBULATED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; LEFT: DRY DARK RED MATERIAL ADRENAL, CORTEX (AC) : -PIGMENT,-MINIMAL TESTIS (TE) : -ATROPHY/DEGENERATION,-SLIGHT >NOTE:>UNILATERAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATE HEART (HT) : -CARDIOMYOPATHY,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 1986 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 237 ANIMAL NUMBER: C92578 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/08/99 STUDY DAY OF DEATH: 598 STUDY WEEK OF DEATH: 86 TERMINAL BODY WEIGHT: 707.7 GRAMS DATE AND TIME OF NECROPSY: 12/08/99 12:52 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUm /s KULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 1987 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 238 ANIMAL NUMBER: C92579 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/12/99 STUDY DAY OF DEATH: 602 STUDY WEEK OF DEATH: 86 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/12/99 14:18 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; PALE; ENTIRE BODY; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); VENTRAL-ABDOMINAL; WEEK 86; VERIFIED AT NECROPSY: NOT OBSERVED >BEHAVIOR; COLD TO TOUCH; ENTIRE BODY; VERIFIED AT NECROPSY: NOT APPLICABLE >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >RESPIRATION; LABORED; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY LIVER (LI) : -LIGHT FOCUS(I)/AREA(S); DIAPHRAGMATIC SURFACE OF LEFT LATERAL LOBE, NEAR MARGIN: SINGLE TAN AREA, 5 X 4 MM; COLLECTED ON ROUTINE SECTION -DIFFUSELY LIGHT; ALL LOBES: TAN KIDNEY (KD) : -ROUGH SURFACE, DIFFUSE; BILATERAL -DIFFUSELY LIGHT; BILATERAL: TAN HISTOPATHOLOGY LIVER (LI) : -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-MODERATE -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -DEGENERATION, CYSTIC,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SEVERE SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MINIMAL PANCREAS (PA) : -INFLAMMATION, VASCULAR,-MODERATE ADRENAL, CORTEX (AC) : -PIGMENT,-MINIMAL ADRENAL, MEDULLA (MA) : -HYPERPLASIA, MEDULLARY,-MINIMAL -B-PHEOCHROMOCYTOMA,-NEOPLASM PRESENT LN, MESENTERIC (MS) : -MINERALIZATION, VASCULAR,-SLIGHT TESTIS (TE) : -INFLAMMATION, VASCULAR,-MINIMAL BONE, FEMUR (FE) : -OSTEODYSTROPHY, FIBROUS,-MODERATE 1988 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 239 ANIMAL NUMBER: C92579 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/12/99 STUDY DAY OF DEATH: 602 STUDY WEEK OF DEATH: 86 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/12/99 14:18 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY HEART (HT) : -DIFFUSELY LIGHT; TAN PARATHYROID (PT) : -LARGE; BILATERAL: EACH IS 2 MM IN DIAMETER STOMACH, GL (ST) : -THICKENED WALL; DIFFUSE CECUM (CE) : -DIFFUSELY RED; MUCOSA MARROW, FEMUR (FM) : -HYPERPLASIA, ERYTHROID, MARROW,-SLIGHT MARROW, STERNUM (SE) : -HYPERPLASIA, ERYTHROID, MARROW,-SLIGHT BONE, STERNUM (SB) : -OSTEODYSTROPHY, FIBROUS,-MINIMAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATELY SEVERE HEART (HT) : -CARDIOMYOPATHY,-MODERATE -MINERALIZATION, VASCULAR,-MODERATE -MINERALIZATION,-MODERATE THYROID (TY) : -DILATATION / CYST, FOLLICULAR,-PRESENT PARATHYROID (PT) : -HYPERPLASIA,-SEVERE PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT -HYPERPLASIA, EPITHELIAL, GLANDULAR,MODERATE -MINERALIZATION, GLANDULAR MUCOSA,MODERATE CECUM (CE) : -EROSION,-MODERATE 1989 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 240 ANIMAL NUMBER: C92579 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/12/99 STUDY DAY OF DEATH: 602 STUDY WEEK OF DEATH: 86 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/12/99 14:18 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY CECUM (CE) : -THICKENED WALL; DIFFUSE SKIN (SK) : -ABRASION(S)/ULCERATION(S); MID VENTRAL ABDOMINAL REGION: SINGLE MOIST TAN ULCERATED AREA, 5 MM IN DIAMETER (CAP 22) LN, RENAL (RL) : -DIFFUSELY RED; RIGHT: 23) GENERAL COMMENT (GC) : -ANIMAL THIN DARK RED (CAP CECUM (CE) : -HEMORRHAGE,-MINIMAL -INFLAMMATION, CHRONIC-ACTIVE,MODERATELY SEVERE SKIN (SK) : -ACANTHOSIS/HYPERKERATOSIS,-SLIGHT -INFLAMMATION, CHRONIC-ACTIVE,-SEVERE -ULCERATION,-MODERATE -FIBROSIS,-MODERATELY SEVERE PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE SEMINAL VESICLES (SV) : -ATROPHY,-MODERATE CAUSE OF DEATH (CD) : -NEPHROPATHY,-PRESENT LN, RENAL (RL) : -HYPERPLASIA, LYMPHOPLASMACYTIC,-SLIGHT -HEMORRHAGE,-PRESENT 1990 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 241 ANIMAL NUMBER: C92579 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/12/99 STUDY DAY OF DEATH: 602 STUDY WEEK OF DEATH: 86 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/12/99 14:18 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAr /i LIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 1991 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 242 ANIMAL NUMBER: C92580 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/20/99 STUDY DAY OF DEATH: 549 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: 503.8 GRAMS DATE AND TIME OF NECROPSY: 10/20/99 11:30 PROSECTOR: JARED FISHEL RECORDER: KEVIN BILLINGS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >EXCRETION; NONFORMED FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >EYES; RED DISCHARGE; EYE-LEFT; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) HISTOPATHOLOGY BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-MODERATELY SEVERE -NECROSIS, COAGULATIVE,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-MODERATE -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL ADRENAL, CORTEX (AC) : -PIGMENT,-MINIMAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),SLIGHT HEART (HT) : -CARDIOMYOPATHY,-SLIGHT 1992 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 243 ANIMAL NUMBER: C92580 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/20/99 STUDY DAY OF DEATH: 549 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: 503.8 GRAMS DATE AND TIME OF NECROPSY: 10/20/99 11:30 PROSECTOR: JARED FISHEL RECORDER: KEVIN BILLINGS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 12 MM IN DIAMETER, DARK RED, TAN AND SOFT STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: MULTIPLE DARK BROWN AREAS, UP TO 2 X 1 MM PROSTATE (PR) : -SMALL; 15 X 10 X 5 MM SEMINAL VESICLES (SV) : -SMALL; BILATERAL: EACH IS 13 X 5 X 2 MM GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; LEFT: DRY AND RED MATERIAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : >UNREMARKABLE PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE SEMINAL VESICLES (SV) : -SECRETION, DECREASED,-PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 1993 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 244 ANIMAL NUMBER: C92580 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/20/99 STUDY DAY OF DEATH: 549 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: 503.8 GRAMS DATE AND TIME OF NECROPSY: 10/20/99 11:30 PROSECTOR: JARED FISHEL RECORDER: KEVIN BILLINGS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 1994 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 245 ANIMAL NUMBER: C92581 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/17/00 STUDY DAY OF DEATH: 729 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 363.3 GRAMS DATE AND TIME OF NECROPSY: 04/17/00 15:34 PROSECTOR: JODI THIEREN RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); LIMB-HIND-RIGHT; WEEK 93; 10 X 10MM; VERIFIED AT NECROPSY: OBSERVED >WART LIKE LESION(S); DORSAL; WEEK 95; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; RED HAIR COAT; HEADENTIRE; VERIFIED AT NECROPSY: NOT OBSERVED >EXCRETION; NO FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; HUNCHED; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) KIDNEY (KD) : -ROUGH SURFACE, DIFFUSE; BILATERAL HISTOPATHOLOGY BRAIN (BR) : ^UNREMARKABLE LIVER (LI) : -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-SLIGHT -DEGENERATION, CYSTIC,-MINIMAL -NECROSIS, COAGULATIVE,-SLIGHT -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL -KARYOMEGALY,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SEVERE SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MINIMAL ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-SLIGHT -PIGMENT,-MINIMAL TESTIS (TE) : -ATROPHY/DEGENERATION,-SLIGHT -INFLAMMATION, VASCULAR,-SLIGHT 1995 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 246 ANIMAL NUMBER: C92581 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/17/00 STUDY DAY OF DEATH: 729 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 363.3 GRAMS DATE AND TIME OF NECROPSY: 04/17/00 15:34 PROSECTOR: JODI THIEREN RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 10 X 6 X 6 MM, DARK RED, TAN AND FIRM STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: MULTIPLE DARK BROWN AREAS, UP TO 2 X 1 MM MARROW, FEMUR (FM) : -HYPOCELLULAR, MARROW,-MODERATELY SEVERE MARROW, STERNUM (SE) : -HYPOCELLULAR, MARROW,-MODERATE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),SLIGHT LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL HEART (HT) : -CARDIOMYOPATHY,-MINIMAL MUSCLE, SKELETAL (SM) : -ATROPHY,-MODERATE NERVE, SCIATIC (SN) : -DEGENERATION,-SLIGHT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL STOMACH, GL (ST) : >UNREMARKABLE STOMACH, NONGL (SU) : -ACANTHOSIS / HYPERKERATOSIS,-MODERATE -ULCERATION,-MINIMAL 1996 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 247 ANIMAL NUMBER: C92581 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/17/00 STUDY DAY OF DEATH: 729 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 363.3 GRAMS DATE AND TIME OF NECROPSY: 04/17/00 15:34 PROSECTOR: JODI THIEREN RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA: MULTIPLE TAN ERODED AREAS, UP TO 3 MM IN DIAMETER SKIN (SK) : -CRUSTED AREA(S); RIGHT LATERAL THORACIC REGION: SINGLE TAN AND DRY AREA, 8 X 5 MM (CAP 24); POSSIBLY CONSISTENT WITH WART-LIKE LESION (DORSAL) AS NOTED IN CLINICAL OBSERVATIONS -MASS(ES); RIGHT THIGH: SINGLE MASS, 17 X 12 X 8 MM, TAN AND FIRM; SAME ON CUT SURFACE (CAP 22); REGIONAL LN (POPLITEAL) NOT IDENTIFIED; OVERLYING SKIN HAS TWO TAN AND DRY ABRADED AREAS, BOTH ARE 3 MM IN DIAMETER; COLLECTED INTACT WITH MASS -MASS #2; RIGHT FLANK: SINGLE MASS, 10 MM IN DIAMETER, TAN AND FIRM; SAME ON CUT SURFACE (CAP 23); REGIONAL LN (INGUINAL) NOT IDENTIFIED PROSTATE (PR) : -NODULAR; SURROUNDING URETHRA: BROWN AND FIRM SEMINAL VESICLES (SV) : -SMALL; BILATERAL: EACH IS 13 X 6 X 2 MM STOMACH, NONGL (SU) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT SKIN (SK) : -CYST, EPIDERMAL INCLUSION,-PRESENT -B-PAPILLOMA, SQUAMOUS CELL,-NEOPLASM PRESENT >NOTE:>PAPILLOMA IS ON SLIDE #24 BOTH MASSES ARE INCLUSION CYSTS PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,MODERATELY SEVERE SEMINAL VESICLES (SV) : -ATROPHY,-SEVERE 1997 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 248 ANIMAL NUMBER: C92581 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/17/00 STUDY DAY OF DEATH: 729 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 363.3 GRAMS DATE AND TIME OF NECROPSY: 04/17/00 15:34 PROSECTOR: JODI THIEREN RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 1998 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 249 ANIMAL NUMBER: C92582 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/21/00 STUDY DAY OF DEATH: 702 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: 569.5 GRAMS DATE AND TIME OF NECROPSY: 03/21/00 13:11 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); SHOULDER-LEFT; WEEK 98; 60 X 40MM; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; SHOULDERLEFT; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; LIMITED USE; LIMB-FRONTLEFT; VERIFIED AT NECROPSY: NOT OBSERVED >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY LIVER (LI) : -LIGHT FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE TAN, FIRM AREAS, UP TO 9 X 5 MM; COLLECTED ON ROUTINE SECTIONS; EXTRA SECTIONS (CAP 22) HISTOPATHOLOGY LIVER (LI) : -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -DEGENERATION, CYSTIC,-SLIGHT -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -HYALINE DROPLETS, TUBULAR EPITHELIUM,MODERATELY SEVERE SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : -VACUOLATION, DIFFUSE,-MODERATE -PIGMENT,-MINIMAL MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,-SLIGHT -HYPERPLASIA, MEGAKARYOCYTIC, MARROW,MODERATE MARROW, STERNUM (SE) : -HYPERPLASIA, MEGAKARYOCYTIC, MARROW,MODERATE 1999 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 250 ANIMAL NUMBER: C92582 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/21/00 STUDY DAY OF DEATH: 702 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: 569.5 GRAMS DATE AND TIME OF NECROPSY: 03/21/00 13:11 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY LUNG (LU) : -LIGHT FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE TAN, FIRM AREAS, UP TO 4 X 2 MM STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA: MULTIPLE TAN ERODED AREAS, UP TO 4 MM IN DIAMETER THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATELY SEVERE -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT LUNG (LU) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT HEART (HT) : -CARDIOMYOPATHY,-MINIMAL ESOPHAGUS (ES) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT THYROID (TY) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT PITUITARY (PI) : -HYPERPLASIA,-MINIMAL STOMACH, NONGL (SU) : -ACANTHOSIS / HYPERKERATOSIS,-MODERATE -ULCERATION,-MODERATE -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE SKIN (SK) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT 2000 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 251 ANIMAL NUMBER: C92582 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/21/00 STUDY DAY OF DEATH: 702 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: 569.5 GRAMS DATE AND TIME OF NECROPSY: 03/21/00 13:11 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY MAMMARY, MALE (MM) : -MASS(ES); LEFT AXILLARY REGION EXTENDS TO LEFT SHOULDER REGION: SINGLE MASS, 45 X 25 X 15 MM, RED, TAN AND FIRM; SAME ON CUT SURFACE; OVERLYING SKIN HAS A BROWN, RED, DRY CRUSTED AREA, 30 X 25 MM; COLLECTED; REGIONAL LN (AXILLARY) NOT IDENTIFIED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN HEMATO NEOPLASIA (HN) : -M-SARCOMA, HISTIOCYTIC,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, HEMATOPOIETIC,-PRESENT MAMMARY, MALE (MM) : >NOT REQUIRED TO BE EXAMINED FOR ANIMAL >NOTE:>SEE SKIN FOR DIAGNOSIS OF MASS 2001 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 252 ANIMAL NUMBER: C92582 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/21/00 STUDY DAY OF DEATH: 702 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: 569.5 GRAMS DATE AND TIME OF NECROPSY: 03/21/00 13:11 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUm /s KULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP) 2002 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 253 ANIMAL NUMBER: C92583 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 577.6 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 10:40 PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); DORSAL; WEEK 74; 15 X 15MM; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; DORSAL; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -LIGHT FOCUS(I)/AREA(S); DIAPHRAGMATIC SURFACE OF RIGHT MEDIAN LOBE: SINGLE TAN AREA, 3 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION KIDNEY (KD) : -ROUGH SURFACE, DIFFUSE; BILATERAL -LARGE; BILATERAL: EACH IS 30 X 20 X 12 MM -DIFFUSELY LIGHT; BILATERAL: TAN ADRENAL, CORTEX (AC) : -LARGE; LEFT: 8 X 6 X 6 MM (RIGHT IS 5 X 4 X 3 MM) HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MODERATE -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL -NECROSIS, COAGULATIVE,-MINIMAL -HYPERPLASIA, BILE DUCT,-SLIGHT -B-ADENOMA, HEPATOCELLULAR,-NEOPLASM PRESENT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SEVERE PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-MODERATE ADRENAL, CORTEX (AC) : -CORTICAL TISSUE, EXTRACAPSULAR,PRESENT ADRENAL, MEDULLA (MA) : -B-PHEOCHROMOCYTOMA,- 2 NEOPLASMS PRESENT TESTIS (TE) : -ATROPHY/DEGENERATION,-MODERATE 2003 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 254 ANIMAL NUMBER: C92583 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 577.6 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 10:40 PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 5 X 5 X 3 MM, DARK RED, TAN AND SOFT STOMACH, GL (ST) : -THICKENED WALL; DIFFUSE SKIN (SK) : -MASS(ES); MID DORSAL LUMBAR REGION: SINGLE MASS, 15 X 15 X 10 MM, TAN AND FIRM; SAME ON CUT SURFACE; OVERLYING SKIN HAS A SINGLE BROWN ULCERATED AREA, 7 MM IN DIAMETER; COLLECTED INTACT WITH MASS; REGIONAL LN (AXILLARY) NORMAL (CAP 25) LN, RENAL (RL) : -LARGE; LEFT: 10 X 6 X 3 MM (RIGHT IS 5 X 4 X 3 MM) -DIFFUSELY RED; BILATERAL: DARK RED (CAP 23) TESTIS (TE) : -INFLAMMATION, VASCULAR,-SEVERE PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : >UNREMARKABLE SKIN (SK) : -M-CARCINOMA, SQUAMOUS CELL,-NEOPLASM PRESENT URINARY BLADDER (UB) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, AXILLARY (AX) : -HYPERPLASIA, LYMPHOPLASMACYTIC,MODERATELY SEVERE LN, RENAL (RL) : -HEMORRHAGE,-PRESENT MAMMARY, MALE (MM) : -GALACTOCELE,-PRESENT 2004 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 255 ANIMAL NUMBER: C92583 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 577.6 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 10:40 PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY MAMMARY, MALE (MM) : -CYST(S); BILATERAL, PECTORAL REGIONS: MULTIPLE TAN CREAMY FLUID FILLED CYSTS, UP TO 15 X 12 X 5 MM (CAP 22) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, MANDIBULAR (MN), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), THYROID (TY), PARATHYROID (PT) 2005 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 256 ANIMAL NUMBER: C92584 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/11/99 STUDY DAY OF DEATH: 418 STUDY WEEK OF DEATH: 60 TERMINAL BODY WEIGHT: 401.8 GRAMS DATE AND TIME OF NECROPSY: 06/11/99 13:56 PROSECTOR: MICHELE MARGGI RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MINIMAL PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-SLIGHT ADRENAL, CORTEX (AC) : -PIGMENT,-MINIMAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATELY SEVERE PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, NONGL (SU) : -ACANTHOSIS / HYPERKERATOSIS,-MODERATE -ULCERATION,-MINIMAL -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL 2006 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 257 ANIMAL NUMBER: C92584 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/11/99 STUDY DAY OF DEATH: 418 STUDY WEEK OF DEATH: 60 TERMINAL BODY WEIGHT: 401.8 GRAMS DATE AND TIME OF NECROPSY: 06/11/99 13:56 PROSECTOR: MICHELE MARGGI RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN -STAINS-PERINEUM/PERIANAL; MOIST MATERIAL YELLOW CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 2007 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 258 ANIMAL NUMBER: C92584 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/11/99 STUDY DAY OF DEATH: 418 STUDY WEEK OF DEATH: 60 TERMINAL BODY WEIGHT: 401.8 GRAMS DATE AND TIME OF NECROPSY: 06/11/99 13:56 PROSECTOR: MICHELE MARGGI RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) (CE), 2008 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 259 ANIMAL NUMBER: C92585 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/12/99 STUDY DAY OF DEATH: 268 STUDY WEEK OF DEATH: 39 TERMINAL BODY WEIGHT: 470.1 GRAMS DATE AND TIME OF NECROPSY: 01/12/98 15:02 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: NOT OBSERVED >APPEARANCE; MISSING; TEETH; VERIFIED AT NECROPSY: OBSERVED >RESPIRATION; LABORED; VERIFIED AT NECROPSY: NOT APPLICABLE >MASS(ES); VENTRAL-CERVICAL; WEEK 37; 30 X 20MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); AXILLARY REGION-RIGHT; WEEK 37; 20 X 20MM; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >MASS(ES); AXILLARY REGION-LEFT; WEEK 39; 10 X 10MM; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY SPLEEN (SP) : -LARGE; 50 X 15 X 10 MM LN, MESENTERIC (MS) : -LARGE; UP TO 10 X 10 X 8 MM HISTOPATHOLOGY LIVER (LI) : -CONGESTION,-PRESENT -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT KIDNEY (KD) : -REGENERATION, TUBULAR EPITHELIUM,MINIMAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT LN, MESENTERIC (MS) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT MARROW, FEMUR (FM) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT MARROW, STERNUM (SE) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT THYMUS (TH) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT 2009 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 260 ANIMAL NUMBER: C92585 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/12/99 STUDY DAY OF DEATH: 268 STUDY WEEK OF DEATH: 39 TERMINAL BODY WEIGHT: 470.1 GRAMS DATE AND TIME OF NECROPSY: 01/12/98 15:02 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY THYMUS (TH) : -MASS(ES); INVOLVING ENTIRE ORGAN: SINGLE MASS, 35 X 35 X 25 MM, DARK RED, TAN, AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (SEE MEDIASTINAL) SEMINAL VESICLES (SV) : -SMALL; BILATERAL: EACH IS 14 X 9 X 1 MM LN, MEDIASTINAL (ML) : -LARGE; UP TO 20 X 20 X 15 MM (CAP 23) LUNG (LU) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT HEART (HT) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT SALIV GL, MANDIB (SG) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT ILEUM (IL) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT SEMINAL VESICLES (SV) : -SECRETION, DECREASED,-PRESENT HEMATO NEOPLASIA (HN) : -M-LYMPHOMA,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, HEMATOPOIETIC,-PRESENT LN, MEDIASTINAL (ML) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT 2010 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 261 ANIMAL NUMBER: C92585 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/12/99 STUDY DAY OF DEATH: 268 STUDY WEEK OF DEATH: 39 TERMINAL BODY WEIGHT: 470.1 GRAMS DATE AND TIME OF NECROPSY: 01/12/98 15:02 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY LN, AXILLARY (AX) : -LARGE; BILATERAL: EACH IS UP TO 40 X 22 X 15 MM (CAP 22); POSSIBLY CONSISTENT WITH MASSES (LEFT AND RIGHT AXILLARY REGIONS) AS NOTED IN CLINICAL OBSERVATIONS LN, INGUINAL (IN) : -LARGE; BILATERAL: EACH IS 11 X 8 X 6 MM (CAP 24) LN, OTHER (LN) : -LARGE; HEPATIC: UP TO 25 X 15 X 15 MM (CAP 25) LN, RENAL (RL) : -LARGE; BILATERAL: EACH IS 25 X 17 X 15 MM (CAP 26) LN, MANDIBULAR (MN) : -LARGE; BILATERAL: EACH IS UP TO 25 X 15 X 12 MM; COLLECTED; POSSIBLY CONSISTENT WITH MASS (VENTRAL CERVICAL) NOTED IN CLINICAL OBSERVATIONS GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -TEETH MISSING/BROKEN/WORN; MISSING, BILATERAL UPPER INCISORS -DISCHARGE-OCULAR; LEFT: DARK RED AND DRY -FLUID-THORACIC CAVITY; CONTAINS A LARGE AMOUNT OF RED FLUID LN, AXILLARY (AX) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT LN, INGUINAL (IN) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT LN, OTHER (LN) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT LN, RENAL (RL) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT LN, MANDIBULAR (MN) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT 2011 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 262 ANIMAL NUMBER: C92585 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/12/99 STUDY DAY OF DEATH: 268 STUDY WEEK OF DEATH: 39 TERMINAL BODY WEIGHT: 470.1 GRAMS DATE AND TIME OF NECROPSY: 01/12/98 15:02 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), EPIDIDYMIDES (EP), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), TESTIS (TE), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), EPIDIDYMIDES (EP) 2012 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 263 ANIMAL NUMBER: C92586 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/07/99 STUDY DAY OF DEATH: 506 STUDY WEEK OF DEATH: 73 TERMINAL BODY WEIGHT: 518.1 GRAMS DATE AND TIME OF NECROPSY: 09/07/99 14:10 PROSECTOR: JERALD W. JOHNSON RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; THIN; VERIFIED AT NECROPSY: NOT OBSERVED >SKIN & PELAGE; YELLOW HAIR COAT; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 9 X 8 X 8 MM, DARK RED, RED, TAN AND SOFT STOMACH, GL (ST) : -RED FOCUS(I)/AREA(S); MUCOSA: MULTIPLE DARK RED AREAS, UP TO 2 X 1 MM HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-MODERATE KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SLIGHT -MINERALIZATION, PELVIC,-MINIMAL -PIGMENT, TUBULAR,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : -PIGMENT,-MINIMAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),SEVERE PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : >UNREMARKABLE 2013 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 264 ANIMAL NUMBER: C92586 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/07/99 STUDY DAY OF DEATH: 506 STUDY WEEK OF DEATH: 73 TERMINAL BODY WEIGHT: 518.1 GRAMS DATE AND TIME OF NECROPSY: 09/07/99 14:10 PROSECTOR: JERALD W. JOHNSON RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY STOMACH, GL (ST) : -THICKENED WALL; DIFFUSE STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA: MULTIPLE TAN ERODED AREAS, UP TO 2 MM IN DIAMETER -THICKENED WALL; DIFFUSE PROSTATE (PR) : -NODULAR; PROXIMAL, SURROUNDING URETHRA: GREEN AND FIRM SEMINAL VESICLES (SV) : -THICKENED; BILATERAL: RED, GRAY AND FIRM GENERAL COMMENT (GC) : -BLOOD FILM TAKEN STOMACH, NONGL (SU) : -ACANTHOSIS / HYPERKERATOSIS,MODERATELY SEVERE -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE SEMINAL VESICLES (SV) : -HYPERPLASIA, EPITHELIAL,-SLIGHT -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 2014 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 265 ANIMAL NUMBER: C92586 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/07/99 STUDY DAY OF DEATH: 506 STUDY WEEK OF DEATH: 73 TERMINAL BODY WEIGHT: 518.1 GRAMS DATE AND TIME OF NECROPSY: 09/07/99 14:10 PROSECTOR: JERALD W. JOHNSON RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAr /i LIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2015 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 266 ANIMAL NUMBER: C92587 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/20/99 STUDY DAY OF DEATH: 610 STUDY WEEK OF DEATH: 88 TERMINAL BODY WEIGHT: 480.5 GRAMS DATE AND TIME OF NECROPSY: 12/20/99 11:13 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; RED HAIR COAT; HEADENTIRE; VERIFIED AT NECROPSY: NOT OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) KIDNEY (KD) : -LARGE PELVIS(ES); BILATERAL -ROUGH SURFACE, DIFFUSE; BILATERAL HISTOPATHOLOGY BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT LIVER (LI) : -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-SLIGHT -DEGENERATION, CYSTIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SEVERE SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-MODERATE -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-SLIGHT -PIGMENT,-MINIMAL ADRENAL, MEDULLA (MA) : -HYPERPLASIA, MEDULLARY,-MODERATE 2016 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 267 ANIMAL NUMBER: C92587 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/20/99 STUDY DAY OF DEATH: 610 STUDY WEEK OF DEATH: 88 TERMINAL BODY WEIGHT: 480.5 GRAMS DATE AND TIME OF NECROPSY: 12/20/99 11:13 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 13 X 10 X 10 MM, RED, DARK RED, GRAY, AND SOFT STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: MULTIPLE BROWN AREAS, UP TO 2 MM IN DIAMETER MAMMARY, MALE (MM) : -CYST(S); BILATERAL PECTORAL REGIONS: MULTIPLE TAN AND CREAMY SEMIFLUID FILLED CYSTS, UP TO 3 MM IN DIAMETER (CAP 22) MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,-MODERATE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),SEVERE THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : >UNREMARKABLE URINARY BLADDER (UB) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SEVERE SEMINAL VESICLES (SV) : -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT MAMMARY, MALE (MM) : -GALACTOCELE,-PRESENT -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL 2017 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 268 ANIMAL NUMBER: C92587 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/20/99 STUDY DAY OF DEATH: 610 STUDY WEEK OF DEATH: 88 TERMINAL BODY WEIGHT: 480.5 GRAMS DATE AND TIME OF NECROPSY: 12/20/99 11:13 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN -DISCHARGE-ORAL; RED AND MOIST THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2018 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 269 ANIMAL NUMBER: C92588 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/03/00 STUDY DAY OF DEATH: 715 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 424.8 GRAMS DATE AND TIME OF NECROPSY: 04/03/00 15:26 PROSECTOR: NANCY DIEDRICH RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; MISSING; TEETH; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: NOT OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) HISTOPATHOLOGY BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, PELVIC,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,SLIGHT -ATROPHY, ACINAR,-MINIMAL -B-ADENOMA, ISLET CELL,-NEOPLASM PRESENT ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-MINIMAL -PIGMENT,-MINIMAL 2019 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 270 ANIMAL NUMBER: C92588 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/03/00 STUDY DAY OF DEATH: 715 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 424.8 GRAMS DATE AND TIME OF NECROPSY: 04/03/00 15:26 PROSECTOR: NANCY DIEDRICH RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 10 MM IN DIAMETER, RED, DARK RED AND SOFT PROSTATE (PR) : -LARGE; 3 5 X 2 0 X 1 0 MM SEMINAL VESICLES (SV) : -LARGE; BILATERAL: EACH IS 40 X 20 X 5 MM TESTIS (TE) : -HYPERPLASIA, INTERSTITIAL CELL (LEYDIG CELL),-MINIMAL MARROW, FEMUR (FM) : -HYPOCELLULAR, MARROW,-SEVERE MARROW, STERNUM (SE) : -HYPOCELLULAR, MARROW,-SLIGHT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATELY SEVERE HEART (HT) : -CARDIOMYOPATHY,-MINIMAL MUSCLE, SKELETAL (SM) : -ATROPHY,-MINIMAL NERVE, SCIATIC (SN) : -DEGENERATION,-MINIMAL PARATHYROID (PT) : >SECTION EXAMINED; TISSUE NOT PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT SEMINAL VESICLES (SV) : -SECRETION, INCREASED,-PRESENT 2020 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 271 ANIMAL NUMBER: C92588 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/03/00 STUDY DAY OF DEATH: 715 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 424.8 GRAMS DATE AND TIME OF NECROPSY: 04/03/00 15:26 PROSECTOR: NANCY DIEDRICH RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY MAMMARY, MALE (MM) : -THICKENED; BILATERAL, AXILLARY REGIONS: TAN AND FIRM (CAP 22) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; RIGHT UPPER INCISOR -TEETH MISSING/BROKEN/WORN; LEFT UPPER INCISOR: MISSING EPIDIDYMIDES (EP) : -VACUOLATION, TUBULAR EPITHELIUM,MODERATE CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT MAMMARY, MALE (MM) : -GALACTOCELE,-PRESENT 2021 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 272 ANIMAL NUMBER: C92588 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/03/00 STUDY DAY OF DEATH: 715 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 424.8 GRAMS DATE AND TIME OF NECROPSY: 04/03/00 15:26 PROSECTOR: NANCY DIEDRICH RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), BONE, LUNG (LU), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), HEMATO NEOPLASIA (HN) STERNUM (SB), SPINAL CORD (SC), STOMACH, GL (ST), STOMACH, NONGL SKIN (SK), URINARY BLADDER (UB), (SU), 2022 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 273 ANIMAL NUMBER: C92589 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/13/00 STUDY DAY OF DEATH: 694 STUDY WEEK OF DEATH: 100 TERMINAL BODY WEIGHT: 394.9 GRAMS DATE AND TIME OF NECROPSY: 03/13/00 13:21 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; THIN; VERIFIED AT NECROPSY: NOT OBSERVED >EYES; RED DISCHARGE; EYES; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; ATAXIC; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) ADRENAL, CORTEX (AC) : -CYST(S); RIGHT: SINGLE CLEAR FLUID FILLED CYST, 2 MM IN DIAMETER HISTOPATHOLOGY BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL -DEGENERATION, CYSTIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATELY SEVERE PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,MODERATELY SEVERE ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT -PIGMENT,-SLIGHT 2023 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 274 ANIMAL NUMBER: C92589 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/13/00 STUDY DAY OF DEATH: 694 STUDY WEEK OF DEATH: 100 TERMINAL BODY WEIGHT: 394.9 GRAMS DATE AND TIME OF NECROPSY: 03/13/00 13:21 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 15 X 10 X 7 MM, DARK RED, TAN AND SOFT PROSTATE (PR) : -NODULAR; PROXIMAL, SURROUNDING URETHRA: GREEN GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DARK RED AND DRY ADRENAL, MEDULLA (MA) : -HYPERPLASIA, MEDULLARY,-MINIMAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATELY SEVERE HEART (HT) : -CARDIOMYOPATHY,-MINIMAL MUSCLE, SKELETAL (SM) : -ATROPHY,-MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,MODERATELY SEVERE CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 2024 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 275 ANIMAL NUMBER: C92589 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/13/00 STUDY DAY OF DEATH: 694 STUDY WEEK OF DEATH: 100 TERMINAL BODY WEIGHT: 394.9 GRAMS DATE AND TIME OF NECROPSY: 03/13/00 13:21 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) (UB), 2025 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 276 ANIMAL NUMBER: C92590 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 834.8 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 12:55 PROSECTOR: TODD ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY ADRENAL, CORTEX (AC) : -LARGE; BILATERAL: EACH IS 8 X 6 X 5 MM TESTIS (TE) : -RED/YELLOW FOCUS(I)/AREA(S); LEFT, BENEATH TUNICA: DIFFUSE -LARGE; LEFT: 40 X 22 X 17 MM (RIGHT IS 22 X 14 X 12 MM) PITUITARY (PI) : -RED FOCUS(I)/AREA(S); DIFFUSE: MULTIPLE DARK RED AREAS, UP TO 2 X 1 MM HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -DEGENERATION, CYSTIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SLIGHT PANCREAS (PA) : -INFILTRATION, FAT, INTERSTITIAL,SLIGHT ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATELY SEVERE TESTIS (TE) : -MINERALIZATION, VASCULAR,-MINIMAL -B-INTERSTITIAL CELL TUMOR,-NEOPLASM PRESENT PITUITARY (PI) : -HYPERPLASIA,-MODERATE 2026 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 277 ANIMAL NUMBER: C92590 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 834.8 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 12:55 PROSECTOR: TODD ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY STOMACH, GL (ST) : -THICKENED WALL; MUCOSA: DIFFUSE GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN STOMACH, GL (ST) : -EDEMA,-MODERATELY SEVERE -INFLAMMATION, ACUTE,-MINIMAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUm /s KULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: ADRENAL, MEDULLA (MA), LUNG (LU), THYROID (TY), PARATHYROID (PT), URINARY BLADDER (UB) 2027 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 278 ANIMAL NUMBER: C92591 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 02/18/00 STUDY DAY OF DEATH: 670 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 02/18/00 7:16 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); INGUINAL REGION-LEFT; WEEK 87; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK 95; 10 X 10MM; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -LARGE; ALL LOBES: MARGINS APPEAR ROUNDED TESTIS (TE) : -CONTAINS FLUID; LEFT: CLEAR FLUID HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SLIGHT PANCREAS (PA) : -INFILTRATION, FAT, INTERSTITIAL,SLIGHT ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-MINIMAL -HYPERTROPHY, CORTICAL CELL,-MINIMAL TESTIS (TE) : -ATROPHY/DEGENERATION,-MODERATELY SEVERE PITUITARY (PI) : -HYPERPLASIA,-MINIMAL JEJUNUM (JE) : >AUTOLYTIC ILEUM (IL) : >AUTOLYTIC CECUM (CE) : >AUTOLYTIC 2028 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 279 ANIMAL NUMBER: C92591 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 02/18/00 STUDY DAY OF DEATH: 670 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 02/18/00 7:16 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY MAMMARY, MALE (MM) : -CYST(S); ALL REGIONS, DIFFUSE: MULTIPLE TAN CREAMY SEMIFLUID FILLED CYSTS, UP TO 16 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION; POSSIBLY CONSISTENT WITH MASSES NOTED IN CLINICAL OBSERVATIONS GENERAL COMMENT (GC) : -DISCHARGE-ORAL; YELLOW AND DRY COLON (CO) : >AUTOLYTIC SKIN (SK) : -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL -CYST, EPIDERMAL INCLUSION,-PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,MODERATELY SEVERE CAUSE OF DEATH (CD) : -INFLAMMATION / INFECTION,-PRESENT MAMMARY, MALE (MM) : >NOT REQUIRED TO BE EXAMINED FOR ANIMAL >NOTE:>SEE SKIN FOR DIAGNOSIS 2029 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 280 ANIMAL NUMBER: C92591 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 02/18/00 STUDY DAY OF DEATH: 670 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 02/18/00 7:16 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), RECTUM (RE), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2030 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 281 ANIMAL NUMBER: C92592 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/20/99 STUDY DAY OF DEATH: 610 STUDY WEEK OF DEATH: 88 TERMINAL BODY WEIGHT: 913.0 GRAMS DATE AND TIME OF NECROPSY: 12/20/99 15:02 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; LIMITED USE; LIMBS-HIND; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -VACUOLATION, HEPATOCELLULAR, MIDZONAL / CENTRILOBULAR,-MINIMAL -HYPERPLASIA, BILE DUCT,-SLIGHT -FIBROSIS, PERIBILIARY,-MINIMAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SLIGHT PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL -INFILTRATION, FAT, INTERSTITIAL,SLIGHT ADRENAL, CORTEX (AC) : -HYPERTROPHY, CORTICAL CELL,-MINIMAL -DEGENERATION, CYSTIC / HEMORRHAGIC,MINIMAL -PIGMENT,-MINIMAL SPINAL CORD (SC) : -NECROSIS / HEMORRHAGE, WHITE MATTER,MODERATELY SEVERE 2031 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 282 ANIMAL NUMBER: C92592 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/20/99 STUDY DAY OF DEATH: 610 STUDY WEEK OF DEATH: 88 TERMINAL BODY WEIGHT: 913.0 GRAMS DATE AND TIME OF NECROPSY: 12/20/99 15:02 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL OBESE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATE HEART (HT) : -CARDIOMYOPATHY,-SLIGHT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT CAUSE OF DEATH (CD) : -DEGENERATION/NECROSIS/HEMORRHAGE, SPINAL CORD/BRAIN,-PRESENT 2032 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 283 ANIMAL NUMBER: C92592 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/20/99 STUDY DAY OF DEATH: 610 STUDY WEEK OF DEATH: 88 TERMINAL BODY WEIGHT: 913.0 GRAMS DATE AND TIME OF NECROPSY: 12/20/99 15:02 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2033 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 284 ANIMAL NUMBER: C92593 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 479.8 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 9:38 PROSECTOR: TROY ELLENBECKER RECORDER: KIMBERLY DURLAND POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT' (%) LF ADRENAL (AD0) RT ADRENAL (AD1) BRAIN (BR) LF KIDNEY (KD0) RT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LF TESTIS (TE0) RT TESTIS (TE1) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) .0279 .0154 2.3137 2.0761 1.9731 15.4451 1.6009 .7002 1.6486 1.6414 .0144 .0110 .0058 % .0032 % .4822 % .4327 % .4112 % 3.2191 % .3337 % .1459 % .3436 % .3421 % .0030 % .0023 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0121 .0067 1.0000 .8973 .8528 6.6755 .6919 .3026 .7125 .7094 .0062 .0048 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC, -MINIMAL -VACUOLATION , HEPATOCELLULAR, PERIPORTAL, -MINIMAL KIDNEY (KD) : -DILATATION, PELVIC,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MINIMAL PANCREAS (PA) -INFILTRATE, LYMPHOHISTIOCYTIC, -MINIMAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2034 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 285 ANIMAL NUMBER: C92593 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 479.8 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 9:38 PROSECTOR: TROY ELLENBECKER RECORDER: KIMBERLY DURLAND POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL: (CAP 21) GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN DARK RED LN, MANDIBULAR (MN) : >NOT REQUIRED TO BE EXAMINED FOR ANIMAL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE) 2035 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 286 ANIMAL NUMBER: C92594 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 02/15/00 STUDY DAY OF DEATH: 667 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 02/15/00 13:15 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -LARGE; ALL LOBES: MARGINS APPEAR ROUNDED HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -B-ADENOMA, HEPATOCELLULAR,-NEOPLASM PRESENT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SLIGHT PANCREAS (PA) : -INFILTRATION, FAT, INTERSTITIAL,SLIGHT ADRENAL, CORTEX (AC) : -HYPERTROPHY, CORTICAL CELL,-MINIMAL -HYPERPLASIA, CORTICAL,-MINIMAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATE PITUITARY (PI) : -HYPERPLASIA,-SEVERE 2036 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 287 ANIMAL NUMBER: C92594 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 02/15/00 STUDY DAY OF DEATH: 667 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 02/15/00 13:15 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -RAISED AREA(S); LEFT ASPECT: SINGLE TAN, SLIGHTLY RAISED FOCUS, 2 MM IN DIAMETER ILEUM (IL) : >AUTOLYTIC CECUM (CE) : >AUTOLYTIC URINARY BLADDER (UB) : >AUTOLYTIC PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT CAUSE OF DEATH (CD) : -UNDETERMINED MICROSCOPICALLY,-PRESENT 2037 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 288 ANIMAL NUMBER: C92594 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 02/15/00 STUDY DAY OF DEATH: 667 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 02/15/00 13:15 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG), GENERAL COMMENT (GC) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM COLON (CO), RECTUM (RE), SKIN (SK), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) (JE), 2038 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 289 ANIMAL NUMBER: C92595 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/23/99 STUDY DAY OF DEATH: 583 STUDY WEEK OF DEATH: 84 TERMINAL BODY WEIGHT: 486.7 GRAMS DATE AND TIME OF NECROPSY: 11/23/99 14:40 PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; SORE/SCAB; DORSALTHORACIC; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); DORSAL; WEEK 73; 25 X 30MM; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >EYES; RED DISCHARGE; EYES; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; PALE; ENTIRE BODY; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; THIN; VERIFIED AT NECROPSY: NOT OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -MASS(ES); WITHIN CEREBRUM: SINGLE MASS, 12 X 8 X 8 MM, TAN AND SOFT; COLLECTED INTACT PANCREAS (PA) : -MASS(ES); WITHIN PANCREAS: SINGLE MASS, 1 0 X 1 0 X 8 MM, DARK RED, TAN AND FIRM; SAME ON CUT SURFACE (CAP 22); REGIONAL LN (SEE PANCREATIC) HISTOPATHOLOGY BRAIN (BR) : -M-GRANULAR CELL TUMOR,-NEOPLASM PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -NECROSIS, INDIVIDUAL HEPATOCYTE,MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -HYPERPLASIA, BILE DUCT,-SLIGHT -FIBROSIS, PERIBILIARY,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL PANCREAS (PA) : -ATROPHY, ACINAR,-SLIGHT -M-CARCINOMA, ISLET CELL,-NEOPLASM PRESENT ADRENAL, CORTEX (AC) : -HYPERTROPHY, CORTICAL CELL,-MODERATE -VACUOLATION, DIFFUSE,-MINIMAL -DEGENERATION, CYSTIC / HEMORRHAGIC,MINIMAL 2039 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 290 ANIMAL NUMBER: C92595 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/23/99 STUDY DAY OF DEATH: 583 STUDY WEEK OF DEATH: 84 TERMINAL BODY WEIGHT: 486.7 GRAMS DATE AND TIME OF NECROPSY: 11/23/99 14:40 PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY SKIN (SK) : -MASS(ES); MID DORSAL THORACIC REGION: SINGLE MASS, 30 X 30 X 12 MM, DARK RED, TAN AND FIRM; SAME ON CUT SURFACE; COLLECTED; OVERLYING SKIN HAS A SINGLE DARK RED AND TAN MOIST ULCERATED AREA, 20 MM IN DIAMETER; COLLECTED INTACT WITH MASS; REGIONAL LN (AXILLARY) NORMAL (CAP 24) SEMINAL VESICLES (SV) : -SMALL; BILATERAL: EACH IS 12 X 7 X 2 MM MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,MODERATELY SEVERE MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,MODERATELY SEVERE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY), SLIGHT HEART (HT) : -CARDIOMYOPATHY,-MINIMAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT SKIN (SK) : -B-ADENOMA, BASAL CELL/SQUAMOUS/SEBACEOUS,-NEOPLASM PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL SEMINAL VESICLES (SV) : -ATROPHY,-SEVERE 2040 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 291 ANIMAL NUMBER: C92595 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/23/99 STUDY DAY OF DEATH: 583 STUDY WEEK OF DEATH: 84 TERMINAL BODY WEIGHT: 486.7 GRAMS DATE AND TIME OF NECROPSY: 11/23/99 14:40 PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY LN, ANT MES/PANC (AP) : -MOTTLED; PANCREATIC: RED AND TAN (CAP 23) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DARK RED AND DRY EPIDIDYMIDES (EP) : -VACUOLATION, TUBULAR EPITHELIUM,MODERATE CAUSE OF DEATH (CD) : -NEOPLASM, BRAIN,-PRESENT LN, AXILLARY (AX) : -HYPERPLASIA, LYMPHOPLASMACYTIC,MODERATE LN, ANT MES/PANC (AP) : -HEMORRHAGE,-PRESENT 2041 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 292 ANIMAL NUMBER: C92595 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/23/99 STUDY DAY OF DEATH: 583 STUDY WEEK OF DEATH: 84 TERMINAL BODY WEIGHT: 486.7 GRAMS DATE AND TIME OF NECROPSY: 11/23/99 14:40 PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), HEMATO NEOPLASIA (HN) (ST), 2042 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 293 ANIMAL NUMBER: C92596 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/23/99 STUDY DAY OF DEATH: 491 STUDY WEEK OF DEATH: 71 TERMINAL BODY WEIGHT: 473.4 GRAMS DATE AND TIME OF NECROPSY: 08/23/99 10:58 PROSECTOR: TONY HANFELD RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; THIN; VERIFIED AT NECROPSY: NOT OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; BROWN HAIR COAT; SCROTUM; VERIFIED AT NECROPSY: NOT OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-SLIGHT -GLYCOGEN, HEPATOCELLULAR, INCREASED,MODERATELY SEVERE KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,MODERATELY SEVERE -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL ADRENAL, CORTEX (AC) : -PIGMENT,-MINIMAL LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT 2043 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 294 ANIMAL NUMBER: C92596 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/23/99 STUDY DAY OF DEATH: 491 STUDY WEEK OF DEATH: 71 TERMINAL BODY WEIGHT: 473.4 GRAMS DATE AND TIME OF NECROPSY: 08/23/99 10:58 PROSECTOR: TONY HANFELD RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 9 X 6 X 5 MM, RED, DARK RED, TAN AND SOFT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 2044 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 295 ANIMAL NUMBER: C92596 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/23/99 STUDY DAY OF DEATH: 491 STUDY WEEK OF DEATH: 71 TERMINAL BODY WEIGHT: 473.4 GRAMS DATE AND TIME OF NECROPSY: 08/23/99 10:58 PROSECTOR: TONY HANFELD RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2045 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 296 ANIMAL NUMBER: C92597 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 670.6 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 13:17 PROSECTOR: GREGORY CODDINGTON RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; SORE/SCAB; PAW-HINDLEFT; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; SWOLLEN; PAW-HIND-LEFT; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY TESTIS (TE) : -LARGE; RIGHT: 25 X 17 X 10 MM (LEFT IS 20 X 13 X 9 MM); BOTH ARE SOFT FOOT/FOOTPAD (FP) : -ABRASION(S)/ULCERATION(S); LEFT HINDFOOT: SINGLE DARK RED, MOIST ABRADED AREA, 8 MM IN DIAMETER; SURROUNDING TISSUE APPEARS SWOLLEN; COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -INFLAMMATION, CHRONIC-ACTIVE, PELVIC,SLIGHT PANCREAS (PA) : -INFILTRATION, FAT, INTERSTITIAL,MINIMAL TESTIS (TE) : -ATROPHY/DEGENERATION,-SEVERE URINARY BLADDER (UB) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT FOOT/FOOTPAD (FP) : -INFLAMMATION, CHRONIC-ACTIVE,-SEVERE -ULCERATION,-MODERATE -ACANTHOSIS / HYPERKERATOSIS,-MODERATE -BACTERIA,-PRESENT 2046 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 297 ANIMAL NUMBER: C92597 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 670.6 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 13:17 PROSECTOR: GREGORY CODDINGTON RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), THYROID (TY), PARATHYROID (PT) 2047 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 298 ANIMAL NUMBER: C92598 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 749.5 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 11:40 PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY PITUITARY (PI) : -RED FOCUS(I)/AREA(S); LEFT ANTERIOR ASPECT: SINGLE DARK RED AREA, 2 MM IN DIAMETER STOMACH, GL (ST) : -THICKENED WALL; DIFFUSE HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-SLIGHT -DEGENERATION, CYSTIC,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MODERATELY SEVERE -CYST,-PRESENT LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : -HYPERPLASIA, EPITHELIAL, GLANDULAR,SLIGHT URINARY BLADDER (UB) : -PROTEINACEOUS MATERIAL, LUMINAL,PRESENT 2048 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 299 ANIMAL NUMBER: C92598 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 749.5 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 11:40 PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAr /i LIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), TESTIS (TE), PARATHYROID (PT) 2049 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 300 ANIMAL NUMBER: C92599 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 05/09/99 STUDY DAY OF DEATH: 385 STUDY WEEK OF DEATH: 55 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 05/09/99 8:39 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -DIFFUSELY DARK; ALL LOBES: BROWN -LIGHT FOCUS(I)/a REA(S); ALL LOBES: MULTIPLE TAN, SLIGHTLY INDISTINCT AREAS, UP TO 3 MM IN DIAMETER; COLLECTED ON ROUTINE SECTIONS TESTIS (TE) : -CONTAINS FLUID; LEFT: CLEAR FLUID; LEFT IS ALSO SOFT HISTOPATHOLOGY LIVER (LI) : -NECROSIS, COAGULATIVE,-SLIGHT -INFLAMMATION, SUBACUTE,-SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT ADRENAL, CORTEX (AC) : -PIGMENT,-MINIMAL TESTIS (TE) : -ATROPHY/DEGENERATION,-SEVERE >NOTE:>UNILATERAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY) MODERATE HEART (HT) : -CARDIOMYOPATHY,-SLIGHT -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL PITUITARY (PI) : -HYPERPLASIA,-SLIGHT URINARY BLADDER (UB) : >UNREMARKABLE 2050 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 301 ANIMAL NUMBER: C92599 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 05/09/99 STUDY DAY OF DEATH: 385 STUDY WEEK OF DEATH: 55 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 05/09/99 8:39 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY URINARY BLADDER (UB) : -SEMIFLUID MATERIAL; DARK BROWN SEMIFLUID GENERAL COMMENT (GC) : -ANIMAL OBESE PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL CAUSE OF DEATH (CD) : -UNDETERMINED MICROSCOPICALLY,-PRESENT 2051 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 302 ANIMAL NUMBER: C92599 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 05/09/99 STUDY DAY OF DEATH: 385 STUDY WEEK OF DEATH: 55 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 05/09/99 8:39 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2052 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 303 ANIMAL NUMBER: C92600 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 471.8 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 13:28 PROSECTOR: TROY ELLENBECKER RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) LF ADRENAL (AD0) RT ADRENAL (AD1) BRAIN (BR) LF KIDNEY (KD0) RT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LF TESTIS (TE0) RT TESTIS (TE1) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) .0349 .0286 2.2319 1.7574 1.7944 15.0729 1.7674 .7466 1.6879 1.6725 ..00101923 .0074 % .0061 % .4731 % .3725 % .3803 % 3.1948 % .3746 % .1582 % .3578 % .3545 % .0020 % .0024 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0156 .0128 1.0000 .7874 .8040 6.7534 .7919 .3345 .7563 .7494 .0042 .0050 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL (CAP 21) GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-SLIGHT KIDNEY (KD) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MANDIBULAR (MN) : >NOT REQUIRED TO BE EXAMINED FOR ANIMAL 2053 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 304 ANIMAL NUMBER: C92600 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 471.8 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 13:28 PROSECTOR: TROY ELLENBECKER RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE) 2054 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 305 ANIMAL NUMBER: C92601 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 827.4 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 11:11 PROSECTOR: TERRI L. WILLIAMS RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >WART LIKE LESION(S); DORSAL; WEEK 104; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY PITUITARY (PI) : -LIGHT FOCUS(I)/AREA(S); LEFT ANTERIOR ASPECT: SINGLE TAN AREA, 1 MM IN DIAMETER STOMACH, GL (ST) : -THICKENED WALL; DIFFUSE HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL PANCREAS (PA) : -INFILTRATION, FAT, INTERSTITIAL,SLIGHT LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PARATHYROID (PT) : -HYPERPLASIA,-MODERATE PITUITARY (PI) : -HYPERPLASIA,-MODERATE STOMACH, GL (ST) : -HYPERPLASIA, EPITHELIAL, GLANDULAR,MODERATE SKIN (SK) : -B-KERATOACANTHOMA,-NEOPLASM PRESENT 2055 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 306 ANIMAL NUMBER: C92601 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 827.4 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 11:11 PROSECTOR: TERRI L. WILLIAMS RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY SKIN (SK) : -CRUSTED AREA(S); RIGHT DORSAL SHOULDER REGION: SINGLE TAN, SLIGHTLY RAISED, CRUSTED AREA, 8 MM IN DIAMETER (CAP 22) PROSTATE (PR) : -NODULAR; PROXIMAL SURROUNDING URETHRA: GREEN AND FIRM GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2056 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 307 ANIMAL NUMBER: C92601 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 827.4 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 11:11 PROSECTOR: TERRI L. WILLIAMS RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: TESTIS (TE), URINARY BLADDER (UB) 2057 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 308 ANIMAL NUMBER: C92602 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 637.6 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 9:52 PROSECTOR: TERRI L. WILLIAMS RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY TESTIS (TE) : -SMALL; RIGHT: 20 X 13 X 3 MM (LEFT IS 22 X 16 X 9 MM) -CONTAINS FLUID; LEFT BENEATH TUNICA: CLEAR FLUID GENERAL COMMENT (GC) : -BLOOD FILM TAKEN HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -NECROSIS, INDIVIDUAL HEPATOCYTE,MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL -FIBROSIS, PERIBILIARY,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL PANCREAS (PA) : -INFILTRATION, FAT, INTERSTITIAL,SLIGHT TESTIS (TE) : -ATROPHY/DEGENERATION,-SEVERE THYROID (TY) : -HYPERPLASIA, C-CELL,-MODERATE CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2058 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 309 ANIMAL NUMBER: C92602 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 637.6 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 9:52 PROSECTOR: TERRI L. WILLIAMS RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PARATHYROID (PT), URINARY BLADDER (UB) 2059 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 310 ANIMAL NUMBER: C92603 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/24/98 STUDY DAY OF DEATH: 127 STUDY WEEK OF DEATH: 19 TERMINAL BODY WEIGHT: 521.4 GRAMS DATE AND TIME OF NECROPSY: 08/24/98 10:16 PROSECTOR: JOHN S. HALFORD RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JOHN S. HALFORD *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; PARALYSIS; LIMBS-HIND; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; COLD TO TOUCH; LIMBS-HIND; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY SPINAL CORD (SC) : -FRACTURE(S); BETWEEN L3 AND L5: SINGLE INDISTINCT FRACTURE; COLLECTED HISTOPATHOLOGY LIVER (LI) : -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-SLIGHT -VACUOLATION, HEPATOCELLULAR, MIDZONAL / CENTRILOBULAR,-MINIMAL -HEMATOPOIESIS, EXTRAMEDULLARY,-SLIGHT KIDNEY (KD) : -REGENERATION, TUBULAR EPITHELIUM,MINIMAL -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MINIMAL -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATELY SEVERE MARROW, FEMUR (FM) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT MARROW, STERNUM (SE) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT SPINAL CORD (SC) : >UNREMARKABLE >NOTE:>SEE VERTEBRA 2060 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 311 ANIMAL NUMBER: C92603 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/24/98 STUDY DAY OF DEATH: 127 STUDY WEEK OF DEATH: 19 TERMINAL BODY WEIGHT: 521.4 GRAMS DATE AND TIME OF NECROPSY: 08/24/98 10:16 PROSECTOR: JOHN S. HALFORD RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JOHN S. HALFORD LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN LUNG (LU) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT RECTUM (RE) : -PARASITISM,-PRESENT HEMATO NEOPLASIA (HN) : -M-LYMPHOMA,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, HEMATOPOIETIC,-PRESENT VERTEBRA/MARROW (VT) : -X-HEMATOPOIETIC NEOPLASIA (SEE HN),NEOPLASM PRESENT 2061 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 312 ANIMAL NUMBER: C92603 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/24/98 STUDY DAY OF DEATH: 127 STUDY WEEK OF DEATH: 19 TERMINAL BODY WEIGHT: 521.4 GRAMS DATE AND TIME OF NECROPSY: 08/24/98 10:16 PROSECTOR: JOHN S. HALFORD RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JOHN S. HALFORD THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAr /i LIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), BONE, STERNUM (SB), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP) 2062 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 313 ANIMAL NUMBER: C92604 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/10/00 STUDY DAY OF DEATH: 631 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: 405.0 GRAMS DATE AND TIME OF NECROPSY: 01/10/00 14:41 PROSECTOR: JAMES T. CALLEN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; ROUGH HAIR COAT; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; BROWN HAIR COAT; VENTRAL-ABDOMINAL; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) ADRENAL, CORTEX (AC) : -LARGE; LEFT: 8 MM IN DIAMETER (RIGHT IS 4 X 3 X 3 MM) HISTOPATHOLOGY BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -INFLAMMATION, SUPPURATIVE, PELVIC,MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MINIMAL PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-SLIGHT ADRENAL, CORTEX (AC) : >UNREMARKABLE ADRENAL, MEDULLA (MA) : -M-MALIGNANT PHEOCHROMOCYTOMA,-NEOPLASM PRESENT MARROW, FEMUR (FM) : -HYPOCELLULAR, MARROW,-MODERATE 2063 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 314 ANIMAL NUMBER: C92604 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/10/00 STUDY DAY OF DEATH: 631 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: 405.0 GRAMS DATE AND TIME OF NECROPSY: 01/10/00 14:41 PROSECTOR: JAMES T. CALLEN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 15 X 10 X 8 MM, DARK RED, RED, LOBULATED AND FIRM SEMINAL VESICLES (SV) : -LARGE; BILATERAL: EACH IS 34 X 17 X 8 MM GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN -STAINS-PERINEUM/PERIANAL; MATERIAL DRY BROWN THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATELY SEVERE LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL HEART (HT) : -CARDIOMYOPATHY,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL SEMINAL VESICLES (SV) : -SECRETION, INCREASED,-PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 2064 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 315 ANIMAL NUMBER: C92604 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/10/00 STUDY DAY OF DEATH: 631 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: 405.0 GRAMS DATE AND TIME OF NECROPSY: 01/10/00 14:41 PROSECTOR: JAMES T. CALLEN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2065 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 316 ANIMAL NUMBER: C92605 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 04/11/00 STUDY DAY OF DEATH: 723 STUDY WEEK OF DEATH: 104 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 04/11/00 14:14 PROSECTOR: JARED FISHEL RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK 87; 40 X 50MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); DORSAL; WEEK 79; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; VENTRALABDOMINAL-RIGHT; VERIFIED AT NECROPSY: NOT OBSERVED >SKIN & PELAGE; SORE/SCAB; DORSAL; VERIFIED AT NECROPSY: NOT OBSERVED >SKIN & PELAGE; RED HAIR COAT; HEADENTIRE; VERIFIED AT NECROPSY: OBSERVED >RESPIRATION; AUDIBLE; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; COLD TO TOUCH; ENTIRE BODY; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY KIDNEY (KD) : -ROUGH SURFACE, DIFFUSE; BILATERAL ADRENAL, CORTEX (AC) : -LARGE ; LEFT: 9 MM IN DIAMETER (RIGHT IS 7 X 4 X 3 MM) HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -DEGENERATION, CYSTIC,-MODERATE -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL -HYPERPLASIA, BILE DUCT,-SLIGHT -FIBROSIS, PERIBILIARY,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SEVERE SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATELY SEVERE ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-SLIGHT ADRENAL, MEDULLA (MA) : -B-PHEOCHROMOCYTOMA,- 2 NEOPLASMS PRESENT LN, MESENTERIC (MS) : -MINERALIZATION, VASCULAR,-MODERATELY SEVERE TESTIS (TE) : -MINERALIZATION, VASCULAR,-MINIMAL 2066 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 317 ANIMAL NUMBER: C92605 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 04/11/00 STUDY DAY OF DEATH: 723 STUDY WEEK OF DEATH: 104 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 04/11/00 14:14 PROSECTOR: JARED FISHEL RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY LUNG (LU) : -UNCOLLAPSED; ALL LOBES BONE, FEMUR (FE) : -OSTEODYSTROPHY, FIBROUS,-MODERATE BONE, STERNUM (SB) : -OSTEODYSTROPHY, FIBROUS,-MINIMAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATELY SEVERE LUNG (LU) : -MINERALIZATION, VASCULAR,-MODERATE -INFILTRATE, MACROPHAGE, ALVEOLAR,SLIGHT -MINERALIZATION, SEPTAL,-SLIGHT HEART (HT) : -CARDIOMYOPATHY,-MODERATE -MINERALIZATION, VASCULAR,-MODERATELY SEVERE -MINERALIZATION,-MODERATE NERVE, SCIATIC (SN) : >TISSUE MISSING THYROID (TY) : -B-ADENOMA, FOLLICULAR CELL,-NEOPLASM PRESENT PARATHYROID (PT) : -HYPERPLASIA,-MODERATELY SEVERE PITUITARY (PI) : -HYPERPLASIA,-SLIGHT 2067 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 318 ANIMAL NUMBER: C92605 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 04/11/00 STUDY DAY OF DEATH: 723 STUDY WEEK OF DEATH: 104 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 04/11/00 14:14 PROSECTOR: JARED FISHEL RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY SKIN (SK) : -MASS(ES); RIGHT DORSAL LUMBAR REGION: SINGLE MASS, 15 MM IN DIAMETER, TAN, FIRM AND FRIABLE; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED MAMMARY, MALE (MM) : -MASS(ES); RIGHT ABDOMINAL REGION: SINGLE MASS, 45 X 35 X 25 MM, TAN AND FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED GENERAL COMMENT (GC) : -DISCHARGE-OCULAR; BILATERAL: DRY RED MATERIAL -DISCHARGE-NASAL; BILATERAL, COVERING ENTIRE HEAD: DRY RED MATERIAL STOMACH, GL (ST) : -HYPERPLASIA, EPITHELIAL, GLANDULAR,MODERATE -MINERALIZATION, GLANDULAR MUCOSA,MODERATE JEJUNUM (JE) : >AUTOLYTIC CECUM (CE) : >AUTOLYTIC SKIN (SK) : -B-KERATOACANTHOMA,-NEOPLASM PRESENT -B-LIPOMA,-NEOPLASM PRESENT >NOTE:>LIPOMA IS MASS DESCRIBED UNDER MAMMARY, MALE PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE CAUSE OF DEATH (CD) : -NEPHROPATHY,-PRESENT MAMMARY, MALE (MM) : >NOT REQUIRED TO BE EXAMINED FOR ANIMAL >NOTE:>SEE SKIN FOR DIAGNOSIS 2068 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 319 ANIMAL NUMBER: C92605 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 04/11/00 STUDY DAY OF DEATH: 723 STUDY WEEK OF DEATH: 104 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 04/11/00 14:14 PROSECTOR: JARED FISHEL RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -TRACHEA-CONTENT FOAMY MATTER; WHITE FOAMY MATERIAL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUm /s KULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), MARROW, FEMUR (FM), MARROW, STERNUM (SE), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), TRACHEA (TR), ESOPHAGUS (ES), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), ILEUM (IL), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), SEMINAL VESICLES EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) (SV), 2069 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 320 ANIMAL NUMBER: C92606 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/13/99 STUDY DAY OF DEATH: 542 STUDY WEEK OF DEATH: 78 TERMINAL BODY WEIGHT: 669.7 GRAMS DATE AND TIME OF NECROPSY: 10/13/99 9:51 PROSECTOR: STEVE VAN ADESTINE RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); SHOULDER-RIGHT; WEEK 66; 10 X 10MM; VERIFIED AT NECROPSY: OBSERVED >WART LIKE LESION(S); NOSE; WEEK 75; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; LIMITED USE; LIMBS-HIND; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; RECUMBENT; STERNAL; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY PANCREAS (PA) : -MASS(ES); WITHIN MID PANCREAS: SINGLE MASS, 10 X 7 X 5 MM, RED, DARK RED, TAN, GRAY, FIRM, AND LOBULATED; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (PANCREATIC) NOT IDENTIFIED HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-SLIGHT -HYPERPLASIA, BILE DUCT,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SLIGHT PANCREAS (PA) : -M-CARCINOMA, ISLET CELL,-NEOPLASM PRESENT ADRENAL, CORTEX (AC) : -PIGMENT,-MINIMAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATE HEART (HT) : -CARDIOMYOPATHY,-MINIMAL MUSCLE, SKELETAL (SM) : -ATROPHY,-MINIMAL 2070 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 321 ANIMAL NUMBER: C92606 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/13/99 STUDY DAY OF DEATH: 542 STUDY WEEK OF DEATH: 78 TERMINAL BODY WEIGHT: 669.7 GRAMS DATE AND TIME OF NECROPSY: 10/13/99 9:51 PROSECTOR: STEVE VAN ADESTINE RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: MULTIPLE DARK BROWN AREAS, UP TO 3 X 2 MM SKIN (SK) : -CRUSTED AREA(S); TIP OF SNOUT: SINGLE DARK RED, DRY, SLIGHTLY RAISED CRUSTED AREA, 2 X 1 MM (CAP 24); CONSISTENT WITH WART-LIKE LESION (NOSE) AS NOTED IN CLINICAL OBSERVATIONS -MASS(ES); RIGHT SHOULDER REGION: SINGLE MASS, 20 X 18 X 6 MM, TAN AND FIRM; ON SECTION EXUDES TAN, CASEOUS SEMISOLID; COLLECTED INTACT; REGIONAL LN (SEE AXILLARY) LN, AXILLARY (AX) : -MOTTLED; BILATERAL: BROWN AND TAN (CAP 23) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -STAINS-PERINEUM/PERIANAL; YELLOW AND MOIST PERINEUM: STOMACH, GL (ST) : >UNREMARKABLE SKIN (SK) : -ACANTHOSIS/HYPERKERATOSIS,-MODERATE -INFLAMMATION, CHRONIC-ACTIVE,MODERATELY SEVERE -CYST, EPIDERMAL INCLUSION,-PRESENT PROSTATE (PR) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL CAUSE OF DEATH (CD) : -NEOPLASM, PANCREAS,-PRESENT LN, AXILLARY (AX) : -HYPERPLASIA, LYMPHOPLASMACYTIC,MODERATE -INFILTRATE, MACROPHAGE, PIGMENTED,SLIGHT 2071 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 322 ANIMAL NUMBER: C92606 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/13/99 STUDY DAY OF DEATH: 542 STUDY WEEK OF DEATH: 78 TERMINAL BODY WEIGHT: 669.7 GRAMS DATE AND TIME OF NECROPSY: 10/13/99 9:51 PROSECTOR: STEVE VAN ADESTINE RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUm /s KULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2072 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 323 ANIMAL NUMBER: C92607 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/10/00 STUDY DAY OF DEATH: 631 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: 423.8 GRAMS DATE AND TIME OF NECROPSY: 01/10/00 14:30 PROSECTOR: WENDY CHERRY RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; ROUGH HAIR COAT; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -MOTTLED; ALL LOBES: BROWN AND TAN KIDNEY (KD) : -LARGE PELVIS(ES); BILATERAL -ROUGH SURFACE, DIFFUSE; BILATERAL HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-MODERATE -GLYCOGEN, HEPATOCELLULAR, INCREASED,MODERATE KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MODERATELY SEVERE -DILATATION, PELVIC,-MODERATE SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-SEVERE ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-MODERATE -PIGMENT,-MINIMAL TESTIS (TE) : -INFLAMMATION, VASCULAR,-MODERATELY SEVERE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),SEVERE 2073 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 324 ANIMAL NUMBER: C92607 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/10/00 STUDY DAY OF DEATH: 631 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: 423.8 GRAMS DATE AND TIME OF NECROPSY: 01/10/00 14:30 PROSECTOR: WENDY CHERRY RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 15 X 12 X 12 MM, DARK RED, TAN AND SOFT STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA: MULTIPLE TAN ERODED AREAS, UP TO 10 X 5 MM PROSTATE (PR) : -NODULAR; DIFFUSE: GREEN AND FIRM GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN HEART (HT) : -CARDIOMYOPATHY,-MODERATE PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-SLIGHT STOMACH, GL (ST) : -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL STOMACH, NONGL (SU) : -ACANTHOSIS / HYPERKERATOSIS,-MODERATE -ULCERATION,-SLIGHT -INFLAMMATION, CHRONIC-ACTIVE,MODERATELY SEVERE PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL EPIDIDYMIDES (EP) : -IMMATURE SPERM FORMS,-MINIMAL CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT MAMMARY, MALE (MM) : -GALACTOCELE,-PRESENT 2074 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 325 ANIMAL NUMBER: C92607 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/10/00 STUDY DAY OF DEATH: 631 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: 423.8 GRAMS DATE AND TIME OF NECROPSY: 01/10/00 14:30 PROSECTOR: WENDY CHERRY RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), HEMATO NEOPLASIA (HN) 2075 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 326 ANIMAL NUMBER: C92608 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/19/99 STUDY DAY OF DEATH: 548 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: 386.6 GRAMS DATE AND TIME OF NECROPSY: 10/19/99 14:16 PROSECTOR: JARED FISHEL RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; ROUGH HAIR COAT; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; HUNCHED; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) HISTOPATHOLOGY BRAIN (BR) : >UNREMARKABLE LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -INFILTRATE, MACROPHAGE, PIGMENTED,SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SLIGHT -MINERALIZATION, PELVIC,-SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-MODERATE -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-SLIGHT -PIGMENT,-SLIGHT ADRENAL, MEDULLA (MA) : -B-PHEOCHROMOCYTOMA,-NEOPLASM PRESENT THYMUS (TH) : >SECTION EXAMINED; TISSUE NOT PRESENT HEART (HT) : -CARDIOMYOPATHY,-MINIMAL 2076 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 327 ANIMAL NUMBER: C92608 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/19/99 STUDY DAY OF DEATH: 548 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: 386.6 GRAMS DATE AND TIME OF NECROPSY: 10/19/99 14:16 PROSECTOR: JARED FISHEL RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 9 MM IN DIAMETER, DARK RED, TAN, AND SOFT SEMINAL VESICLES (SV) : -SMALL; RIGHT: 20 X 10 X 3 MM (LEFT IS 34 X 18 X 5 MM) -GELATINOUS; RIGHT: CLEAR AND SOFT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL LOWER INCISORS -DISCHARGE-OCULAR; LEFT: DRY RED MATERIAL -ANIMAL THIN -STAINS-PERINEUM/PERIANAL; BROWN AND YELLOW MOIST MATERIAL THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE SEMINAL VESICLES (SV) : -SECRETION, INCREASED,-PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 2077 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 328 ANIMAL NUMBER: C92608 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/19/99 STUDY DAY OF DEATH: 548 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: 386.6 GRAMS DATE AND TIME OF NECROPSY: 10/19/99 14:16 PROSECTOR: JARED FISHEL RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2078 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 329 ANIMAL NUMBER: C92609 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/12/00 STUDY DAY OF DEATH: 724 STUDY WEEK OF DEATH: 104 TERMINAL BODY WEIGHT: 675.2 GRAMS DATE AND TIME OF NECROPSY: 04/12/00 14:00 PROSECTOR: JARED FISHEL RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >WART LIKE LESION(S); HEAD-MAXILLARYLEFT; WEEK 98; CORNER OF LEFT EYE; VERIFIED AT NECROPSY: NOT OBSERVED >MASS(ES); DORSAL; WEEK 102; 10 X 10MM; VERIFIED AT NECROPSY: OBSERVED >EYES; CLEAR DISCHARGE; EYE-LEFT; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; LIMITED USE; LIMBS-HIND; VERIFIED AT NECROPSY: NOT APPLICABLE >RESPIRATION; LABORED; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -DEPRESSED AREA(S); LEFT FRONTAL CORTEX: DUE TO MASS HISTOPATHOLOGY EYE (EY) : -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL BRAIN (BR) : -M-MENINGEAL SARCOMA,-NEOPLASM PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -HYPERPLASIA, BILE DUCT,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT PANCREAS (PA) : -INFILTRATION, FAT, INTERSTITIAL,SLIGHT ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-MINIMAL -PIGMENT,-SLIGHT MARROW, FEMUR (FM) : -HYPOCELLULAR, MARROW,-SEVERE 2079 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 330 ANIMAL NUMBER: C92609 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/12/00 STUDY DAY OF DEATH: 724 STUDY WEEK OF DEATH: 104 TERMINAL BODY WEIGHT: 675.2 GRAMS DATE AND TIME OF NECROPSY: 04/12/00 14:00 PROSECTOR: JARED FISHEL RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY LUNG (LU) : -MOTTLED; ALL LOBES: RED AND TAN PITUITARY (PI) : -LARGE; 7 X 5 X 3 MM; APPEARS SWOLLEN SKIN (SK) : -MASS(ES); LEFT DORSAL THORACIC REGION: SINGLE MASS, 12 MM IN DIAMETER, TAN AND FIRM; SAME ON CUT SURFACE (CAP 23); REGIONAL LN (SEE AXILLARY) PROSTATE (PR) : -NODULAR; PROXIMAL, SURROUNDING URETHRA: GREEN AND FIRM LN, AXILLARY (AX) : -DIFFUSELY RED; LEFT: DARK RED (CAP 22) THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATE LUNG (LU) : >UNREMARKABLE HEART (HT) : -CARDIOMYOPATHY,-MODERATE PITUITARY (PI) : -I-SARCOMA,-FROM BRAIN HARDERIAN GLAND (HG) : -I-SARCOMA,-FROM BRAIN SKIN (SK) : -B-FIBROMA,-NEOPLASM PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SEVERE CAUSE OF DEATH (CD) : -NEOPLASM, BRAIN,-PRESENT LN, AXILLARY (AX) : -HEMORRHAGE,-PRESENT -HYPERPLASIA, LYMPHOPLASMACYTIC,MODERATE CALVARIUM/SKULL (CA) : -I-SARCOMA,-FROM BRAIN 2080 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 331 ANIMAL NUMBER: C92609 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/12/00 STUDY DAY OF DEATH: 724 STUDY WEEK OF DEATH: 104 TERMINAL BODY WEIGHT: 675.2 GRAMS DATE AND TIME OF NECROPSY: 04/12/00 14:00 PROSECTOR: JARED FISHEL RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY CALVARIUM/SKULL (CA) : -MASS(ES); WITHIN CRANIAL VAULT, ATTACHED TO MENINGES ABOVE LEFT FRONTAL CORTEX: SINGLE MASS, 14 X 11 X 3 MM, TAN AND SOFT; COLLECTED INTACT WITH SKULL CAP GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; LEFT: CLEAR AND MOIST MATERIAL 2081 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 332 ANIMAL NUMBER: C92609 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/12/00 STUDY DAY OF DEATH: 724 STUDY WEEK OF DEATH: 104 TERMINAL BODY WEIGHT: 675.2 GRAMS DATE AND TIME OF NECROPSY: 04/12/00 14:00 PROSECTOR: JARED FISHEL RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2082 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 333 ANIMAL NUMBER: C92610 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 318.8 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 8:57 PROSECTOR: JODI THIEREN RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN STATUS LF ADRENAL (AD0) RT ADRENAL (AD1) BRAIN (BR) LF KIDNEY (KD0) RT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LF TESTIS (TE0) RT TESTIS (TE1) LF THYROID/PARA RT THYROID/PARA (TT0) (TT1) ------------------------------------ 13.5220 ------------------------------------------- ------------------------------------ 4.2417 % ------------------------------------------- ------------------------------------------------------------------------------------- NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN WEIGHT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS 2083 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 334 ANIMAL NUMBER: C92610 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 318.8 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 8:57 PROSECTOR: JODI THIEREN RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) 2084 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 335 ANIMAL NUMBER: C92611 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/20/99 STUDY DAY OF DEATH: 610 STUDY WEEK OF DEATH: 88 TERMINAL BODY WEIGHT: 988.9 GRAMS DATE AND TIME OF NECROPSY: 12/20/99 11:47 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); VENTRAL-ABDOMINAL-RIGHT; WEEK 85; 120 X 120MM; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -DIFFUSELY LIGHT; ALL LOBES: LIGHT BROWN HISTOPATHOLOGY LIVER (LI) : -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-SLIGHT -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, PERIPORTAL,-SLIGHT -CYST, BILIARY,-PRESENT KIDNEY (KD) : -ECTASIA, TUBULAR,-SLIGHT SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATELY SEVERE PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL -INFILTRATION, FAT, INTERSTITIAL,MINIMAL MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,-MODERATE -HYPERPLASIA, ERYTHROID, MARROW,MODERATE MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,-MODERATE -HYPERPLASIA, ERYTHROID, MARROW,MODERATE 2085 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 336 ANIMAL NUMBER: C92611 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/20/99 STUDY DAY OF DEATH: 610 STUDY WEEK OF DEATH: 88 TERMINAL BODY WEIGHT: 988.9 GRAMS DATE AND TIME OF NECROPSY: 12/20/99 11:47 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY THYROID (TY) : -LARGE; LEFT: 6 X 5 X 5 MM (RIGHT IS 4 X 3 X 3 MM) PITUITARY (PI) : -RAISED AREA(S); MID ASPECT: SINGLE DARK RED AND TAN AREA, 2 X 1 X 1 MM MAMMARY, MALE (MM) : -MASS(ES); RIGHT INGUINAL REGION: SINGLE MASS, 140 X 100 X 60 MM (478.0 GRAMS), RED, DARK RED, TAN, LOBULATED, AND FIRM; SOME SECTIONS CONTAIN A DARK RED FLUID; PORTION COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN THYMUS (TH) : >SECTION EXAMINED; TISSUE NOT PRESENT LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL HEART (HT) : -CARDIOMYOPATHY,-MINIMAL THYROID (TY) : -B-ADENOMA, FOLLICULAR CELL,-NEOPLASM PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT SKIN (SK) : -M-FIBROSARCOMA,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, SKIN/MUSCLE/SUBCUTIS,PRESENT MAMMARY, MALE (MM) : >NOT REQUIRED TO BE EXAMINED FOR ANIMAL >NOTE:>SEE SKIN FOR DIAGNOSIS 2086 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 337 ANIMAL NUMBER: C92611 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/20/99 STUDY DAY OF DEATH: 610 STUDY WEEK OF DEATH: 88 TERMINAL BODY WEIGHT: 988.9 GRAMS DATE AND TIME OF NECROPSY: 12/20/99 11:47 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE),ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUm /s KULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE),ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) (TR), 2087 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 338 ANIMAL NUMBER: C92612 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/18/99 STUDY DAY OF DEATH: 547 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: 476.5 GRAMS DATE AND TIME OF NECROPSY: 10/18/99 13:11 PROSECTOR: JARED FISHEL RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; ROUGH HAIR COAT; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; RECUMBENT; LATERAL; VERIFIED AT NECROPSY: NOT APPLICABLE >RESPIRATION; IRREGULAR; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; COLD TO TOUCH; ENTIRE BODY; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) THYROID (TY) : -LARGE; RIGHT: 8 X 5 X 5 MM (LEFT IS 4 X 2 X 1 MM) HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL -INFLAMMATION, CHRONIC-ACTIVE, PELVIC,SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL -ATROPHY, ACINAR,-MINIMAL -B-ADENOMA, ISLET CELL,-NEOPLASM PRESENT ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-MINIMAL THYMUS (TH) : >SECTION EXAMINED; TISSUE NOT PRESENT HEART (HT) : -CARDIOMYOPATHY,-MINIMAL THYROID (TY) : -M-CARCINOMA, FOLLICULAR CELL,-NEOPLASM PRESENT 2088 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 339 ANIMAL NUMBER: C92612 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/18/99 STUDY DAY OF DEATH: 547 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: 476.5 GRAMS DATE AND TIME OF NECROPSY: 10/18/99 13:11 PROSECTOR: JARED FISHEL RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 12 X 8 X 6 MM, RED, DARK RED, TAN, AND SOFT AUDITORY SEB GL (AS) : -MASS(ES); RIGHT: SINGLE MASS, 17 X 10 X 8 MM, TAN, LOBULATED, AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT WITH HEAD; REGIONAL LN (MANDIBULAR) NORMAL (CAP 22) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; RIGHT: DRY RED MATERIAL -ANIMAL THIN PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, NONGL (SU) : -ACANTHOSIS / HYPERKERATOSIS,-MODERATE -ULCERATION,-MINIMAL -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT LN, MANDIBULAR (MN) : -HYPERPLASIA, LYMPHOPLASMACYTIC,MODERATE AUDITORY SEB GL (AS) : -M-CARCINOMA,-NEOPLASM PRESENT 2089 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 340 ANIMAL NUMBER: C92612 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/18/99 STUDY DAY OF DEATH: 547 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: 476.5 GRAMS DATE AND TIME OF NECROPSY: 10/18/99 13:11 PROSECTOR: JARED FISHEL RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2090 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 341 ANIMAL NUMBER: C92613 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 319.6 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 9:28 PROSECTOR: LYNN HORNER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN STATUS LF ADRENAL (AD0) RT ADRENAL (AD1) BRAIN (BR) LF KIDNEY (KD0) RT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LF TESTIS (TE0) RT TESTIS (TE1) LF THYROID/PARA RT THYROID/PARA (TT0) (TT1) ------------------------------------ 13.1820 ------------------------------------------- ------------------------------------ 4.1251 % ------------------------------------------- ------------------------------------------------------------------------------------- NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN WEIGHT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS 2091 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 342 ANIMAL NUMBER: C92613 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 319.6 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 9:28 PROSECTOR: LYNN HORNER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) 2092 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 343 ANIMAL NUMBER: C92614 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 01/19/00 STUDY DAY OF DEATH: 640 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 01/19/00 7:39 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); DORSAL; WEEK 89; 15 X 15MM; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; DORSAL; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY HISTOPATHOLOGY EYE (EY) : >AUTOLYTIC LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SLIGHT PANCREAS (PA) : >AUTOLYTIC ADRENAL, CORTEX (AC) : -PIGMENT,-SLIGHT TESTIS (TE) : -ATROPHY/DEGENERATION,-SEVERE >NOTE:>UNILATERAL THYMUS (TH) : >SECTION EXAMINED; TISSUE NOT PRESENT LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL HEART (HT) : -CARDIOMYOPATHY,-MINIMAL 2093 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 344 ANIMAL NUMBER: C92614 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 01/19/00 STUDY DAY OF DEATH: 640 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 01/19/00 7:39 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -LARGE; 6 X 5 X 3 MM, APPEARS SWOLLEN SKIN (SK) : -CRUSTED AREA(S); MID DORSAL CERVICAL REGION: SINGLE DRY DARK RED CRUSTED AREA, 30 X 20 MM; COLLECTED; POSSIBLY CONSISTENT WITH MASS (DORSAL) NOTED IN CLINICAL OBSERVATION THYROID (TY) : >AUTOLYTIC PARATHYROID (PT) : -HYPERPLASIA,-MODERATELY SEVERE PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : >AUTOLYTIC DUODENUM (DU) : >AUTOLYTIC JEJUNUM (JE) : >AUTOLYTIC ILEUM (IL) : >AUTOLYTIC CECUM (CE) : >AUTOLYTIC SKIN (SK) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE -ULCERATION,-MODERATE -B-FIBROMA,-NEOPLASM PRESENT URINARY BLADDER (UB) : >AUTOLYTIC PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT CAUSE OF DEATH (CD) : -NEOPLASM, SKIN/MUSCLE/SUBCUTIS,PRESENT 2094 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 345 ANIMAL NUMBER: C92614 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 01/19/00 STUDY DAY OF DEATH: 640 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 01/19/00 7:39 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL: DARK RED; COLLECTED LN, MANDIBULAR (MN) : -CONGESTION,-PRESENT THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG), GENERAL COMMENT (GC) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: BRAIN (BR), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), COLON (CO), RECTUM (RE), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2095 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 346 ANIMAL NUMBER: C92615 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/03/00 STUDY DAY OF DEATH: 624 STUDY WEEK OF DEATH: 90 TERMINAL BODY WEIGHT: 633.0 GRAMS DATE AND TIME OF NECROPSY: 01/03/00 8:04 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; ROUGH HAIR COAT; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; RECUMBENT; LATERAL; VERIFIED AT NECROPSY: NOT APPLICABLE >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) TESTIS (TE) : -SMALL; RIGHT: 18 X 15 X 5 MM (LEFT IS 23 X 15 X 10 MM); BOTH ARE SOFT -CONTAINS FLUID; BILATERAL: CLEAR FLUID HISTOPATHOLOGY BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-MODERATE -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL -HYPERPLASIA, ISLET CELLS,-MINIMAL -INFILTRATION, FAT, INTERSTITIAL,MINIMAL TESTIS (TE) : -ATROPHY/DEGENERATION,-MODERATELY SEVERE >NOTE:>BILATERAL 2096 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 347 ANIMAL NUMBER: C92615 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/03/00 STUDY DAY OF DEATH: 624 STUDY WEEK OF DEATH: 90 TERMINAL BODY WEIGHT: 633.0 GRAMS DATE AND TIME OF NECROPSY: 01/03/00 8:04 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 17 X 11 X 10 MM, RED, DARK RED, GRAY, AND SOFT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DARK RED AND DRY -DISCHARGE-NASAL; DARK RED AND DRY THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),SEVERE HEART (HT) : -CARDIOMYOPATHY,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 2097 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 348 ANIMAL NUMBER: C92615 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/03/00 STUDY DAY OF DEATH: 624 STUDY WEEK OF DEATH: 90 TERMINAL BODY WEIGHT: 633.0 GRAMS DATE AND TIME OF NECROPSY: 01/03/00 8:04 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2098 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 349 ANIMAL NUMBER: C92616 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 401.2 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 10:26 PROSECTOR: LAURIE J. SCHULLER RECORDER: KIMBERLY DURLAND POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT' (%) LF ADRENAL (AD0) RT ADRENAL (AD1) BRAIN (BR) LF KIDNEY (KD0) RT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LF TESTIS (TE0) RT TESTIS (TE1) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) .0196 .0158 2.0788 1.5410 1.6127 11.5117 1.7953 .5603 1.5787 1.5658 .0102 .0099 .0049 % .0039 % .5181 % .3841 % .4020 % 2.8693 % .4475 % .1397 % .3935 % .3903 % .0025 % .0025 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0094 .0076 1.0000 .7413 .7758 5.5377 .8636 .2695 .7594 .7532 .0049 .0048 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED KIDNEY (KD) : -LARGE PELVIS(ES); RIGHT HARDERIAN GLAND (HG) : -MISSING/NOT IDENTIFIED; LEFT: NOT IDENTIFIED; RIGHT APPEARS NORMAL LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -NECROSIS, INDIVIDUAL HEPATOCYTE,MINIMAL KIDNEY (KD) : -DILATATION, PELVIC,-MODERATE ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-MINIMAL HARDERIAN GLAND (HG) : >NOT REQUIRED TO BE EXAMINED FOR ANIMAL STOMACH, GL (ST) : >NOT REQUIRED TO BE EXAMINED FOR ANIMAL 2099 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 350 ANIMAL NUMBER: C92616 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 401.2 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 10:26 PROSECTOR: LAURIE J. SCHULLER RECORDER: KIMBERLY DURLAND POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: MULTIPLE DARK BROWN PINPOINT FOCI GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUm /s KULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE) 2100 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 351 ANIMAL NUMBER: C92617 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/05/99 STUDY DAY OF DEATH: 565 STUDY WEEK OF DEATH: 81 TERMINAL BODY WEIGHT: 503.2 GRAMS DATE AND TIME OF NECROPSY: 11/05/99 7:09 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >EYES; PALE; EYES; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; NO FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; RECUMBENT; LATERAL; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >EYES; RED DISCHARGE; EYES; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY EYE (EY) : -WHITE INTRAOCULAR MATERIAL; BILATERAL; POSSIBLY CONSISTENT WITH PALE EYES AS NOTED IN CLINICAL OBSERVATIONS BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -LIGHT FOCUS(I)/AREA(S); DIAPHRAGMATIC SURFACE OF LEFT LATERAL LOBE: SINGLE TAN AREA, 17 X 11 MM; COLLECTED ON ROUTINE SECTION KIDNEY (KD) : -ROUGH SURFACE, DIFFUSE; BILATERAL HISTOPATHOLOGY EYE (EY) : -DEGENERATION, LENTICULAR,-MODERATE BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-SLIGHT -HYPERPLASIA, BILE DUCT,-MINIMAL -B-ADENOMA, HEPATOCELLULAR,-NEOPLASM PRESENT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MODERATELY SEVERE SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-MODERATE -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL 2101 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 352 ANIMAL NUMBER: C92617 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/05/99 STUDY DAY OF DEATH: 565 STUDY WEEK OF DEATH: 81 TERMINAL BODY WEIGHT: 503.2 GRAMS DATE AND TIME OF NECROPSY: 11/05/99 7:09 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 11 X 10 X 9 MM, RED, DARK RED, GREY, TAN, LOBULATED AND SOFT STOMACH, GL (ST) : -RED FOCUS(I)/AREA(S); MUCOSA, NEAR PYLORIC VALVE: SINGLE DARK RED FOCUS, 2 MM IN DIAMETER SEMINAL VESICLES (SV) : -GELATINOUS; BILATERAL: CLEAR AND SOFT GENERAL COMMENT (GC) -BLOOD FILM TAKEN -DISCHARGE-OCULAR; MATERIAL -ANIMAL THIN : BILATERAL: DRY, RED ADRENAL, CORTEX (AC) : -PIGMENT,-MINIMAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),SEVERE LUNG (LU) : -INFLAMMATION, SUBACUTE,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL STOMACH, GL (ST) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT SEMINAL VESICLES (SV) : >UNREMARKABLE CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 2102 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 353 ANIMAL NUMBER: C92617 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/05/99 STUDY DAY OF DEATH: 565 STUDY WEEK OF DEATH: 81 TERMINAL BODY WEIGHT: 503.2 GRAMS DATE AND TIME OF NECROPSY: 11/05/99 7:09 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAr /i LIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2103 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 354 ANIMAL NUMBER: C92618 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 319.7 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 10:23 PROSECTOR: LYNN HORNER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN STATUS LF ADRENAL (AD0) RT ADRENAL (AD1) BRAIN (BR) LF KIDNEY (KD0) RT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LF TESTIS (TE0) RT TESTIS (TE1) LF THYROID/PARA RT THYROID/PARA (TT0) (TT1) ------------------------------------ 14.8145 ------------------------------------------- ------------------------------------ 4.6341 % ------------------------------------------- ------------------------------------------------------------------------------------- NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN WEIGHT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS 2104 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 355 ANIMAL NUMBER: C92618 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 319.7 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 10:23 PROSECTOR: LYNN HORNER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) 2105 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 356 ANIMAL NUMBER: C92619 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 684.5 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 13:00 PROSECTOR: TROY ELLENBECKER RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -DEGENERATION, CYSTIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL -FIBROSIS, PERIBILIARY,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SLIGHT PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL -HYPERPLASIA, ISLET CELLS,-SLIGHT -ATROPHY, ACINAR,-SLIGHT -HYPERTROPHY / BASOPHILIA, ACINAR CELL,SLIGHT THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL -B-ADENOMA, FOLLICULAR CELL,-NEOPLASM PRESENT 2106 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 357 ANIMAL NUMBER: C92619 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 684.5 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 13:00 PROSECTOR: TROY ELLENBECKER RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY STOMACH, GL (ST) : -THICKENED WALL; DIFFUSE GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN STOMACH, GL (ST) : -HYPERPLASIA, EPITHELIAL, GLANDULAR,MODERATE CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: TESTIS (TE), LUNG (LU), PARATHYROID (PT), URINARY BLADDER (UB) 2107 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 358 ANIMAL NUMBER: C92620 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 02/15/00 STUDY DAY OF DEATH: 667 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 02/15/00 7:23 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; MISSING; TEETH; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); SINGLE MASS, 9 X 8 X 5 MM RED, DARK RED, TAN, GRAY AND SOFT HISTOPATHOLOGY BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -DILATATION, PELVIC,-SLIGHT -MINERALIZATION, PELVIC,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : -PIGMENT,-MINIMAL THYMUS (TH) : >SECTION EXAMINED; TISSUE NOT PRESENT LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL THYROID (TY) : -DILATATION / CYST, FOLLICULAR,-PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT 2108 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 359 ANIMAL NUMBER: C92620 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 02/15/00 STUDY DAY OF DEATH: 667 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 02/15/00 7:23 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PREPUTIAL GLAND (PG) : -LARGE; BILATERAL: LEFT IS 6 X 6 X 3 MM, RIGHT IS 5 X 4 X 3 MM; BOTH APPEAR CYSTIC WITH GREEN SEMISOLID (CAP 22) GENERAL COMMENT (GC) : -MALOCCLUSION; BILATERAL: LOWER INCISORS -TEETH MISSING/BROKEN/WORN; BILATERAL UPPER INCISORS: BROKEN OFF AT BASE -DISCHARGE-OCULAR; BILATERAL: DARK RED AND DRY HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL STOMACH, GL (ST) : -EDEMA,-SLIGHT -INFLAMMATION, ACUTE,-SLIGHT COLON (CO) : -PARASITISM,-PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT PREPUTIAL GLAND (PG) : -INFLAMMATION, CHRONIC-ACTIVE,MODERATELY SEVERE 2109 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 360 ANIMAL NUMBER: C92620 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 02/15/00 STUDY DAY OF DEATH: 667 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 02/15/00 7:23 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2110 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 361 ANIMAL NUMBER: C92621 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 526.8 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 9:51 PROSECTOR: STEVE VAN ADESTINE RECORDER: KIMBERLY DURLAND POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT' (%) LF ADRENAL (AD0) RT ADRENAL (AD1) BRAIN (BR) LF KIDNEY (KD0) RT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LF TESTIS (TE0) RT TESTIS (TE1) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) .0379 .0377 2.1646 1.9881 1.8534 18.7242 1.9337 .8626 1.6258 1.6364 .0117 .0145 .0072 % .0072 % .4109 % .3774 % .3518 % 3.5543 % .3671 % .1637 % .3086 % .3106 % .0022 % .0028 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0175 .0174 1.0000 .9185 .8562 8.6502 .8933 .3985 .7511 .7560 .0054 .0067 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED ADRENAL, CORTEX (AC) : >NOTE:>TRIMMING COMMENT: ONE ADRENAL MISSING FROM WET TISSUE AT TIME OF TRIMMING LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -NECROSIS, INDIVIDUAL HEPATOCYTE,MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MINIMAL PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL ADRENAL, CORTEX (AC) : -UNILATERALLY EXAMINED,-PRESENT 2111 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 362 ANIMAL NUMBER: C92621 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 526.8 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 9:51 PROSECTOR: STEVE VAN ADESTINE RECORDER: KIMBERLY DURLAND POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY ADRENAL, MEDULLA (MA) : >NOTE:>TRIMMING COMMENT: ONE ADRENAL MISSING FROM WET TISSUE AT TIME OF TRIMMING LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL: DARK RED (CAP 21) GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN GENERAL INFORMATION (XX) : >NOTE:>SODIUM PENTOBARBITAL ADMINISTERED PRIOR TO CARBON DIOXIDE ANESTHESIA ADRENAL, MEDULLA (MA) : -UNILATERALLY EXAMINED,-PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MANDIBULAR (MN) : >NOT REQUIRED TO BE EXAMINED FOR ANIMAL 2112 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 363 ANIMAL NUMBER: C92621 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 526.8 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 9:51 PROSECTOR: STEVE VAN ADESTINE RECORDER: KIMBERLY DURLAND POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), LN, MESENTERIC (MS), TESTIS (TE) 2113 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 364 ANIMAL NUMBER: C92622 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/18/99 STUDY DAY OF DEATH: 578 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/18/99 7:52 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LN, MESENTERIC (MS) -DIFFUSELY RED HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -DEGENERATION, CYSTIC,-MINIMAL -ANGIECTASIS,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SLIGHT PANCREAS (PA) : -ATROPHY, ACINAR,-MINIMAL -HYPERTROPHY / BASOPHILIA, ACINAR CELL,MINIMAL ADRENAL, CORTEX (AC) : -PIGMENT,-MINIMAL ADRENAL, MEDULLA (MA) : -B-PHEOCHROMOCYTOMA,- 2 NEOPLASMS PRESENT LN, MESENTERIC (MS) : -HEMORRHAGE,-PRESENT TESTIS (TE) : -MINERALIZATION, VASCULAR,-MINIMAL MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,-SLIGHT 2114 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 365 ANIMAL NUMBER: C92622 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/18/99 STUDY DAY OF DEATH: 578 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/18/99 7:52 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY HEART (HT) : -MASS(ES); INVOLVING RIGHT AND LEFT ATRIA: SINGLE MASS, 12 MM IN DIAMETER, TAN AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (SEE MEDIASTINAL) STOMACH, GL (ST) : -THICKENED WALL; DIFFUSE LN, MEDIASTINAL (ML) : -DIFFUSELY RED; DARK RED (CAP 22) GENERAL INFORMATION (XX) : >NOTE:>ANIMAL IDENTIFICATION TRANSPONDER DAMAGED AT NECROPSY THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATE HEART (HT) : -M-ATRIOCAVAL MESOTHELIOMA,-NEOPLASM PRESENT THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT -FIBROSIS,-SLIGHT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT CAUSE OF DEATH (CD) : -NEOPLASM, HEART,-PRESENT LN, MEDIASTINAL (ML) : -HEMORRHAGE,-PRESENT -INFILTRATE, MAST CELL,-MODERATE 2115 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 366 ANIMAL NUMBER: C92622 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/18/99 STUDY DAY OF DEATH: 578 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/18/99 7:52 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG), GENERAL COMMENT (GC) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), BONE, FEMUR (FE), MARROW, FEMUR (FM), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2116 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 367 ANIMAL NUMBER: C92623 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 703.8 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 7:59 PROSECTOR: TERRI L. WILLIAMS RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE DARK RED AREAS, UP TO 5 MM IN DIAMETER; COLLECTED ON ROUTINE SECTIONS PITUITARY (PI) : -RED FOCUS(I)/AREA(S); LEFT POSTERIOR ASPECT: SINGLE DARK RED AREA, 2 MM IN DIAMETER STOMACH, GL (ST) : -THICKENED WALL; DIFFUSE HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -DEGENERATION, CYSTIC,-MINIMAL -VACUOLATION, HEPATOCELLULAR,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL PANCREAS (PA) : -ATROPHY, ACINAR,-MINIMAL -INFILTRATION, FAT, INTERSTITIAL,SLIGHT THYROID (TY) : -DILATATION / CYST, FOLLICULAR,-PRESENT PITUITARY (PI) : -HYPERPLASIA,-SLIGHT STOMACH, GL (ST) : -HYPERPLASIA, EPITHELIAL, GLANDULAR,MODERATE URINARY BLADDER (UB) : -PROTEINACEOUS MATERIAL, LUMINAL,PRESENT 2117 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 368 ANIMAL NUMBER: C92623 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 703.8 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 7:59 PROSECTOR: TERRI L. WILLIAMS RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUm /s KULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: TESTIS (TE), LUNG (LU), PARATHYROID (PT) 2118 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 369 ANIMAL NUMBER: C92624 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 06/18/99 STUDY DAY OF DEATH: 425 STUDY WEEK OF DEATH: 61 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 06/18/99 14:44 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 10 X 7 X 6 MM, RED, DARK RED, TAN AND SOFT HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-MINIMAL ADRENAL, CORTEX (AC) : -PIGMENT,-MINIMAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT JEJUNUM (JE) : >AUTOLYTIC ILEUM (IL) : >AUTOLYTIC CECUM (CE) : >AUTOLYTIC 2119 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 370 ANIMAL NUMBER: C92624 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 06/18/99 STUDY DAY OF DEATH: 425 STUDY WEEK OF DEATH: 61 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 06/18/99 14:44 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -DISCHARGE-NASAL; BILATERAL: RED AND MOIST MATERIAL COLON (CO) : >AUTOLYTIC URINARY BLADDER (UB) : -PROTEINACEOUS MATERIAL, LUMINAL,PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 2120 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 371 ANIMAL NUMBER: C92624 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 06/18/99 STUDY DAY OF DEATH: 425 STUDY WEEK OF DEATH: 61 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 06/18/99 14:44 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), RECTUM SKIN (SK), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) (RE), 2121 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 372 ANIMAL NUMBER: C92625 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/28/99 STUDY DAY OF DEATH: 588 STUDY WEEK OF DEATH: 84 TERMINAL BODY WEIGHT: 460.6 GRAMS DATE AND TIME OF NECROPSY: 11/28/99 13:56 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; THIN; VERIFIED AT NECROPSY: NOT OBSERVED >SKIN & PELAGE; PALE; ENTIRE BODY; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; TREMORS; HEAD; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; COLD TO TOUCH; ENTIRE BODY; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; HUNCHED; VERIFIED AT NECROPSY: NOT APPLICABLE >RESPIRATION; IRREGULAR; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY KIDNEY (KD) : -CYST(S); BILATERAL, CORTICAL SURFACE: MULTIPLE CLEAR FLUID FILLED CYSTS, UP TO 10 MM IN DIAMETER -ROUGH SURFACE, DIFFUSE; BILATERAL HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -DEGENERATION, CYSTIC,-MINIMAL -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SEVERE -DILATATION, PELVIC,-SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL -HYPERTROPHY / BASOPHILIA, ACINAR CELL,SLIGHT ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-SLIGHT -HYPERTROPHY, CORTICAL CELL,-SLIGHT -HYPERPLASIA, CORTICAL,-MINIMAL 2122 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 373 ANIMAL NUMBER: C92625 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/28/99 STUDY DAY OF DEATH: 588 STUDY WEEK OF DEATH: 84 TERMINAL BODY WEIGHT: 460.6 GRAMS DATE AND TIME OF NECROPSY: 11/28/99 13:56 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -RED FOCUS(I)/AREA(S); LEFT POSTERIOR ASPECT: SINGLE DARK RED FOCUS, 1 MM IN DIAMETER STOMACH, GL (ST) : -THICKENED WALL; MUCOSA, DIFFUSE ADRENAL, MEDULLA (MA) : -HYPERPLASIA, MEDULLARY,-MODERATELY SEVERE LN, MESENTERIC (MS) : >TISSUE MISSING TESTIS (TE) : -ATROPHY/DEGENERATION,-SLIGHT BONE, FEMUR (FE) : -OSTEODYSTROPHY, FIBROUS,-SLIGHT BONE, STERNUM (SB) : -OSTEODYSTROPHY, FIBROUS,-SLIGHT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY) MODERATELY SEVERE HEART (HT) : -CARDIOMYOPATHY,-MODERATE THYROID (TY) : -B-ADENOMA, FOLLICULAR CELL,-NEOPLASM PRESENT PARATHYROID (PT) : -HYPERPLASIA,-MODERATELY SEVERE PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT -CYST,-PRESENT STOMACH, GL (ST) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT 2123 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 374 ANIMAL NUMBER: C92625 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/28/99 STUDY DAY OF DEATH: 588 STUDY WEEK OF DEATH: 84 TERMINAL BODY WEIGHT: 460.6 GRAMS DATE AND TIME OF NECROPSY: 11/28/99 13:56 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN STOMACH, GL (ST) : -HYPERPLASIA, EPITHELIAL, GLANDULAR,SLIGHT -MINERALIZATION, GLANDULAR MUCOSA,MODERATELY SEVERE CECUM (CE) : -EROSION,-SLIGHT -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT URINARY BLADDER (UB) : -PROTEINACEOUS MATERIAL, LUMINAL,PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL CAUSE OF DEATH (CD) : -NEPHROPATHY,-PRESENT 2124 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 375 ANIMAL NUMBER: C92625 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/28/99 STUDY DAY OF DEATH: 588 STUDY WEEK OF DEATH: 84 TERMINAL BODY WEIGHT: 460.6 GRAMS DATE AND TIME OF NECROPSY: 11/28/99 13:56 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUm /s KULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), MARROW, FEMUR (FM), MARROW, STERNUM (SE), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), COLON (CO), RECTUM (RE), SKIN (SK), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2125 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 376 ANIMAL NUMBER: C92626 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 726.2 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 10:55 PROSECTOR: JAMES T. CALLEN RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; MISSING; TEETH; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; PAW-HINDLEFT; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; SWOLLEN; PAW-HIND-LEFT; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY FOOT/FOOTPAD (FP) : -ABRASION(S)/ULCERATION(S); LEFT HINDFOOT: SINGLE MOIST, RED ABRADED AREA, 15 MM IN DIAMETER; COLLECTED HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL PANCREAS (PA) : -INFILTRATION, FAT, INTERSTITIAL,MINIMAL TESTIS (TE) : -ATROPHY/DEGENERATION,-MINIMAL LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,SLIGHT THYROID (TY) : -B-ADENOMA, FOLLICULAR CELL,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT FOOT/FOOTPAD (FP) : -INFLAMMATION, CHRONIC-ACTIVE,MODERATELY SEVERE -ULCERATION,-MODERATE 2126 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 377 ANIMAL NUMBER: C92626 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 726.2 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 10:55 PROSECTOR: JAMES T. CALLEN RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY JOINT, OTHER (JT) : -LARGE; LEFT TARSAL: UP TO 12 MM IN DIAMETER; COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -MALOCCLUSION; LEFT UPPER INCISOR -TEETH MISSING/BROKEN/WORN; RIGHT UPPER INCISOR: MISSING -DISCHARGE-OCULAR; RIGHT: DRY RED MATERIAL JOINT, OTHER (JT) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE 2127 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 378 ANIMAL NUMBER: C92626 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 726.2 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 10:55 PROSECTOR: JAMES T. CALLEN RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PARATHYROID (PT), URINARY BLADDER (UB) 2128 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 379 ANIMAL NUMBER: C92627 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 525.3 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 13:32 PROSECTOR: LAURIE J. SCHULLER RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT' (%) LF ADRENAL (AD0) RT ADRENAL (AD1) BRAIN (BR) LF KIDNEY (KD0) RT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LF TESTIS (TE0) RT TESTIS (TE1) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) .0277 .0262 2.3192 2.0656 1.9527 16.9241 1.8755 1.0427 2.0320 1.9782 .0148 .0192 .0053 % .0050 % .4415 % .3932 % .3717 % 3.2218 % .3570 % .1985 % .3868 % .3766 % .0028 % .0037 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0119 .0113 1.0000 .8907 .8420 7.2974 .8087 .4496 .8762 .8530 .0064 .0083 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-SLIGHT KIDNEY (KD) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL PANCREAS (PA) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2129 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 380 ANIMAL NUMBER: C92627 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 525.3 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 13:32 PROSECTOR: LAURIE J. SCHULLER RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE) 2130 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 381 ANIMAL NUMBER: C92628 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/07/99 STUDY DAY OF DEATH: 506 STUDY WEEK OF DEATH: 73 TERMINAL BODY WEIGHT: 471.9 GRAMS DATE AND TIME OF NECROPSY: 09/07/99 13:45 PROSECTOR: JERALD W. JOHNSON RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; THIN; VERIFIED AT NECROPSY: NOT OBSERVED >SKIN & PELAGE; ROUGH HAIR COAT; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY EYE (EY) : >NOTE:>LEFT RUPTURED AT NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) ADRENAL, CORTEX (AC) : -SMALL; BILATERAL: EACH IS 3 X 2 X 2 MM HISTOPATHOLOGY BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT -INFILTRATE, MACROPHAGE, PIGMENTED,SLIGHT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL ADRENAL, CORTEX (AC) : -PIGMENT,-SLIGHT -ATROPHY, CORTICAL,-MODERATELY SEVERE MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,-SLIGHT MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,-SLIGHT 2131 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 382 ANIMAL NUMBER: C92628 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/07/99 STUDY DAY OF DEATH: 506 STUDY WEEK OF DEATH: 73 TERMINAL BODY WEIGHT: 471.9 GRAMS DATE AND TIME OF NECROPSY: 09/07/99 13:45 PROSECTOR: JERALD W. JOHNSON RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 11 X 9 X 7 MM, DARK RED, GRAY AND SOFT STOMACH, GL (ST) : -EROSION/ULCERATION; MUCOSA: SINGLE TAN ERODED AREA, 2 X 1 MM -THICKENED WALL; DIFFUSE PROSTATE (PR) : -NODULAR; PROXIMAL, SURROUNDING URETHRA: GREEN, BROWN AND FIRM SEMINAL VESICLES (SV) : -LARGE; BILATERAL: EACH IS 25 X 15 X 5 MM GENERAL COMMENT (GC) : -BLOOD FILM TAKEN THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATELY SEVERE LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL MUSCLE, SKELETAL (SM) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT SEMINAL VESICLES (SV) : -SECRETION, INCREASED,-PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 2132 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 383 ANIMAL NUMBER: C92628 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/07/99 STUDY DAY OF DEATH: 506 STUDY WEEK OF DEATH: 73 TERMINAL BODY WEIGHT: 471.9 GRAMS DATE AND TIME OF NECROPSY: 09/07/99 13:45 PROSECTOR: JERALD W. JOHNSON RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAr /i LIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2133 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 384 ANIMAL NUMBER: C92629 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 10/17/99 STUDY DAY OF DEATH: 546 STUDY WEEK OF DEATH: 78 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 10/17/99 9:10 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY KIDNEY (KD) : -ROUGH SURFACE, DIFFUSE; BILATERAL -LARGE; BILATERAL: EACH IS 32 X 26 X 19 MM -DIFFUSELY LIGHT; BILATERAL: TAN ADRENAL, CORTEX (AC) : -LARGE; RIGHT: 5 MM IN DIAMETER (LEFT IS 4 MM IN DIAMETER) TESTIS (TE) : -SMALL; BILATERAL: EACH IS 19 X 15 X 15 MM; BOTH ARE SOFT HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SEVERE SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL -B-ADENOMA, ACINAR CELL,-NEOPLASM PRESENT ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-SLIGHT -ANGIECTASIS,-MODERATE -PIGMENT,-SLIGHT TESTIS (TE) : -ATROPHY/DEGENERATION,-MODERATELY SEVERE -INFLAMMATION, VASCULAR,-SLIGHT 2134 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 385 ANIMAL NUMBER: C92629 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 10/17/99 STUDY DAY OF DEATH: 546 STUDY WEEK OF DEATH: 78 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 10/17/99 9:10 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY STOMACH, GL (ST) : -RED FOCUS(I)/AREA(S); MUCOSA: MULTIPLE RED PINPOINT FOCI -THICKENED WALL; MUCOSA THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATE LUNG (LU) : -LEUKOCYTES, INCREASED, INTRAVASCULAR,PRESENT -MINERALIZATION, SEPTAL,-SLIGHT HEART (HT) : -CARDIOMYOPATHY,-MODERATE -MINERALIZATION, VASCULAR,-MODERATE PARATHYROID (PT) : -HYPERPLASIA,-MODERATE STOMACH, GL (ST) : -HYPERPLASIA, EPITHELIAL, GLANDULAR,SLIGHT -MINERALIZATION, GLANDULAR MUCOSA,MODERATE ILEUM (IL) : >AUTOLYTIC CECUM (CE) : >AUTOLYTIC PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE EPIDIDYMIDES (EP) : -IMMATURE SPERM FORMS,-SLIGHT -HYPOSPERMIA,-PRESENT CAUSE OF DEATH (CD) : -NEPHROPATHY,-PRESENT 2135 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 386 ANIMAL NUMBER: C92629 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 10/17/99 STUDY DAY OF DEATH: 546 STUDY WEEK OF DEATH: 78 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 10/17/99 9:10 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY AORTA (AO) : -LARGE; DIFFUSE: UP TO 5 MM IN DIAMETER -FIRM; DIFFUSE GENERAL COMMENT (GC) : -ANIMAL THIN -URINARY BLADDER-LARGE LUMEN; UP TO 18 MM IN DIAMETER -DISCHARGE-ORAL; CLEAR, MOIST MATERIAL AORTA (AO) : -MINERALIZATION,-MODERATELY SEVERE 2136 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 387 ANIMAL NUMBER: C92629 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 10/17/99 STUDY DAY OF DEATH: 546 STUDY WEEK OF DEATH: 78 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 10/17/99 9:10 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), HEMATO NEOPLASIA (HN) 2137 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 388 ANIMAL NUMBER: C92630 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/26/99 STUDY DAY OF DEATH: 616 STUDY WEEK OF DEATH: 88 TERMINAL BODY WEIGHT: 427.7 GRAMS DATE AND TIME OF NECROPSY: 12/26/99 7:19 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); VENTRAL-ABDOMINAL-RIGHT; WEEK 73; 10 X 10MM; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >EYES; RED DISCHARGE; EYES; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; ATAXIC; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY KIDNEY (KD) : -CYST(S); BILATERAL, DIFFUSE: MULTIPLE CLEAR FLUID FILLED CYSTS, UP TO 3 MM IN DIAMETER -LARGE PELVIS(ES); BILATERAL -DIFFUSELY LIGHT; BILATERAL: TAN ADRENAL, CORTEX (AC) : -LARGE; LEFT: 10 X 8 X 6 MM (RIGHT IS 5 X 4 X 4 MM) HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -DEGENERATION, CYSTIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-SLIGHT -FIBROSIS, PERIBILIARY,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SEVERE -HYPERPLASIA, TRANSITIONAL CELL,MODERATE -INFLAMMATION, CHRONIC-ACTIVE, PELVIC,SLIGHT -CYST,-PRESENT SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SLIGHT ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-SLIGHT -PIGMENT,-MINIMAL ADRENAL, MEDULLA (MA) : -M-MALIGNANT PHEOCHROMOCYTOMA,-NEOPLASM PRESENT LN, MESENTERIC (MS) : >TISSUE MISSING 2138 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 389 ANIMAL NUMBER: C92630 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/26/99 STUDY DAY OF DEATH: 616 STUDY WEEK OF DEATH: 88 TERMINAL BODY WEIGHT: 427.7 GRAMS DATE AND TIME OF NECROPSY: 12/26/99 7:19 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY TESTIS (TE) : -SMALL; RIGHT: 20 X 15 X 8 MM (LEFT IS 25 X 17 X 10 MM); RIGHT IS SOFT STOMACH, GL (ST) : -THICKENED WALL; DIFFUSE SEMINAL VESICLES (SV) : -SMALL; BILATERAL: EACH IS 17 X 10 X 3 MM TESTIS (TE) : -ATROPHY/DEGENERATION,-MODERATELY SEVERE -INFLAMMATION, VASCULAR,-SLIGHT >NOTE:>UNILATERAL BONE, FEMUR (FE) : -OSTEODYSTROPHY, FIBROUS,-MINIMAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATELY SEVERE HEART (HT) : -CARDIOMYOPATHY,-SLIGHT PARATHYROID (PT) : -HYPERPLASIA,-SEVERE PITUITARY (PI) : -HYPERPLASIA,-MINIMAL STOMACH, GL (ST) : -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL -HYPERPLASIA, EPITHELIAL, GLANDULAR,MODERATELY SEVERE SKIN (SK) : -CYST, EPIDERMAL INCLUSION,-PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,MODERATELY SEVERE SEMINAL VESICLES (SV) : -ATROPHY,-MODERATELY SEVERE 2139 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 390 ANIMAL NUMBER: C92630 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/26/99 STUDY DAY OF DEATH: 616 STUDY WEEK OF DEATH: 88 TERMINAL BODY WEIGHT: 427.7 GRAMS DATE AND TIME OF NECROPSY: 12/26/99 7:19 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY LN, RENAL (RL) : -DIFFUSELY RED; BILATERAL: DARK RED (CAP 22) MAMMARY, MALE (MM) : -CYST(S); RIGHT PERINEAL REGION: TWO TAN AND CREAMY SEMIFLUID FILLED CYSTS, ONE IS 15 MM IN DIAMETER, THE OTHER IS 5 MM IN DIAMETER; COLLECTED; POSSIBLY CONSISTENT WITH MASS NOTED IN CLINICAL OBSERVATIONS LN, LUMBAR/ILIAC (LM) : -CYST(S); RIGHT: SINGLE CLEAR FLUID FILLED CYST, 6 MM IN DIAMETER (CAP 23) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DARK RED AND DRY -ANIMAL THIN CAUSE OF DEATH (CD) : -NEPHROPATHY,-PRESENT LN, RENAL (RL) : -HYPERPLASIA, LYMPHOPLASMACYTIC,MODERATE MAMMARY, MALE (MM) : >NOT REQUIRED TO BE EXAMINED FOR ANIMAL >NOTE:>SEE SKIN FOR DIAGNOSIS LN, LUMBAR/ILIAC (LM) : -HYPERPLASIA, LYMPHOPLASMACYTIC,-SLIGHT -HEMORRHAGE,-PRESENT 2140 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 391 ANIMAL NUMBER: C92630 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/26/99 STUDY DAY OF DEATH: 616 STUDY WEEK OF DEATH: 88 TERMINAL BODY WEIGHT: 427.7 GRAMS DATE AND TIME OF NECROPSY: 12/26/99 7:19 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, MANDIBULAR (MN), COAGULATING GL (CG), TAIL (TI), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2141 COVAH^ THE DEVELOPMENT SERVICES COMPANY Sponsor: 3M St. Paul, Minnesota FINAL REPORT Study Title: 104-Week Dietary Chronic Toxicity and Carcinogenicity Study with Perfluorooctane Sulfonic Acid Potassium Salt (PFOS; T-6295) in Rats Author: Peter J. Thomford, PhD Study Completion Date: January 2, 2002 Testing Facility: Covance Laboratories Inc. 3301 Kinsman Boulevard Madison, Wisconsin 53704-2595 Laboratory Study Identification: Covance 6329-183 Sponsor Study Identification: 3M T-6295 Volume VI of IX Page 2142 of 4068 Covance 6329-183 3M T-6295 VOLUME VI OF IX CONTENTS Page APPENDIX 6 (Continued)........................................................................................... 2144 Individual Anatomic Pathology Data....................................................................... 2145 2143 APPENDIX 6 (Continued) Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 2144 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 392 ANIMAL NUMBER: C92631 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 765.4 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 11:10 PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -VACUOLATION, HEPATOCELLULAR, MIDZONAL / CENTRILOBULAR,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MODERATELY SEVERE -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-SLIGHT -HYPERPLASIA, BILE DUCT,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SLIGHT PANCREAS (PA) : -INFILTRATION, FAT, INTERSTITIAL,MODERATE TESTIS (TE) : -B-INTERSTITIAL CELL TUMOR,-NEOPLASM PRESENT LUNG (LU) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERPLASIA, TYPE II PNEUMOCYTE,MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-MODERATE 2145 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 393 ANIMAL NUMBER: C92631 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 765.4 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 11:10 PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY STOMACH, GL (ST) : -THICKENED WALL; DIFFUSE GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN STOMACH, GL (ST) : -HYPERPLASIA, EPITHELIAL, GLANDULAR,MODERATE URINARY BLADDER (UB) : -PROTEINACEOUS MATERIAL, LUMINAL,PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2146 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 394 ANIMAL NUMBER: C92631 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 765.4 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 11:10 PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PARATHYROID (PT) 2147 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 395 ANIMAL NUMBER: C92632 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/03/00 STUDY DAY OF DEATH: 715 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 650.4 GRAMS DATE AND TIME OF NECROPSY: 04/03/00 14:45 PROSECTOR: JOHN S. HALFORD RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; SORE/SCAB; EAR-LEFT; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); EAR-LEFT; WEEK 48; 30 X 30MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); VENTRAL-CERVICAL-LEFT; WEEK 103; 25 X 45MM; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: NOT OBSERVED >RESPIRATION; LABORED; VERIFIED AT NECROPSY: NOT APPLICABLE >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; COLD TO TOUCH; ENTIRE BODY; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY ADRENAL, CORTEX (AC) : -LARGE; RIGHT: 7 MM IN DIAMETER (LEFT IS 4 MM IN DIAMETER) THYMUS (TH) : -MISSING/NOT IDENTIFIED; NOT IDENTIFIED: POSSIBLE REMNANTS COLLECTED HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL -FIBROSIS, PERIBILIARY,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SLIGHT ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-SLIGHT ADRENAL, MEDULLA (MA) : -B-PHEOCHROMOCYTOMA,-NEOPLASM PRESENT TESTIS (TE) : -ATROPHY/DEGENERATION,-MINIMAL -HYPERPLASIA, INTERSTITIAL CELL (LEYDIG CELL),-MINIMAL MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,-MODERATE MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,MODERATELY SEVERE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATELY SEVERE 2148 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 396 ANIMAL NUMBER: C92632 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/03/00 STUDY DAY OF DEATH: 715 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 650.4 GRAMS DATE AND TIME OF NECROPSY: 04/03/00 14:45 PROSECTOR: JOHN S. HALFORD RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY LUNG (LU) : -LIGHT FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE GRAY, FIRM AND SLIGHTLY RAISED AREAS, UP TO 9 X 7 X 3 MM HEART (HT) : -LIGHT FOCUS(I)/AREA(S); EPICARDIUM OF LEFT VENTRICLE: SINGLE TAN AREA, 3 X 2 X 1 MM PITUITARY (PI) : -LARGE; 4 X 3 X 2 MM; APPEARS SWOLLEN STOMACH, GL (ST) : -THICKENED WALL; MUCOSA: DIFFUSE LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL -N-SARCOMA,-NEOPLASM PRESENT HEART (HT) : -CARDIOMYOPATHY,-SLIGHT -N-SARCOMA,-NEOPLASM PRESENT THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT SALIV GL, MANDIB (SG) : -I-SARCOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : >UNREMARKABLE SKIN (SK) : -M-FIBROSARCOMA,-NEOPLASM PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT CAUSE OF DEATH (CD) : -NEOPLASM, SKIN/MUSCLE/SUBCUTIS,PRESENT LN, MANDIBULAR (MN) : -HYPERPLASIA, LYMPHOPLASMACYTIC,-SLIGHT AUDITORY SEB GL (AS) : -I-SARCOMA,-NEOPLASM PRESENT 2149 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 397 ANIMAL NUMBER: C92632 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/03/00 STUDY DAY OF DEATH:: 715 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 650.4 GRAMS DATE AND TIME OF NECROPSY: 04/03/00 14:45 PROSECTOR: JOHN S. HALFORD RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY AUDITORY SEB GL (AS) : -MASS(ES); LEFT, INVOLVING ENTIRE ORGAN, EXTENDING TO LEFT VENTRAL CERVICAL REGION AND LEFT PINNA: SINGLE MASS, 57 X 23 X 18 MM, RED, DARK RED, GRAY, TAN, VASCULAR, LOBULATED AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (MANDIBULAR) NORMAL (CAP 22); SKIN OVERLYING LEFT PINNA HAS A SINGLE DRY BLACK CRUSTED AREA, 28 X 25 X 19 MM; COLLECTED INTACT WITH MASS PERICARDIAL SAC (PD) : -MASS(ES); INVOLVING ENTIRE TISSUE: SINGLE MASS, 34 X 24 X 14 MM, RED, DARK RED, GRAY, TAN, LOBULATED AND FIRM; SAME ON CUT SURFACE (CAP 23); REGIONAL LN (MEDIASTINAL) NOT IDENTIFIED; PORTIONS OF MASS ADHERED TO DIAPHRAGM; COLLECTED INTACT WITH MASS GENERAL COMMENT (GC) : -BLOOD FILM TAKEN PERICARDIAL SAC (PD) : -N-SARCOMA,-NEOPLASM PRESENT 2150 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 398 ANIMAL NUMBER: C92632 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/03/00 STUDY DAY OF DEATH: 715 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 650.4 GRAMS DATE AND TIME OF NECROPSY: 04/03/00 14:45 PROSECTOR: JOHN S. HALFORD RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), LN, MESENTERIC (MS), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2151 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 399 ANIMAL NUMBER: C92633 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 830.9 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 13:20 PROSECTOR: TODD ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; PAW-HINDRIGHT; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY TESTIS (TE) : -SMALL; LEFT: 20 X 13 X 4 MM (RIGHT IS 25 X 15 X 12 MM); LEFT APPEARS SOFT HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL -FIBROSIS, PERIBILIARY,-MINIMAL -B-ADENOMA, HEPATOCELLULAR,-NEOPLASM PRESENT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL PANCREAS (PA) : -ATROPHY, ACINAR,-MODERATE -INFILTRATION, FAT, INTERSTITIAL,MODERATELY SEVERE TESTIS (TE) : -ATROPHY/DEGENERATION,-SEVERE >NOTE:>UNILATERAL LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL 2152 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 400 ANIMAL NUMBER: C92633 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 830.9 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 13:20 PROSECTOR: TODD ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -RED FOCUS(I)/AREA(S); LEFT AND RIGHT ASPECT: SINGLE DARK RED AREA ON EACH, BOTH ARE 2 X 1 MM STOMACH, GL (ST) : -EROSION/ULCERATION; MUCOSA: SINGLE DARK BROWN ERODED AREA, 4 X 1 MM -THICKENED WALL; MUCOSA: DIFFUSE PROSTATE (PR) : -NODULAR; PROXIMAL SURROUNDING URETHRA: GREEN FOOT/FOOTPAD (FP) : -CRUSTED AREA(S); RIGHT HINDFOOT: SINGLE DARK RED AND DRY AREA, 6 X 5 MM; COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -MALOCCLUSION; BILATERAL UPPER INCISORS THYROID (TY) : -M-CARCINOMA, FOLLICULAR CELL,-NEOPLASM PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT -ULCERATION,-SLIGHT -HYPERPLASIA, EPITHELIAL, GLANDULAR,MODERATE PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT FOOT/FOOTPAD (FP) : -INFLAMMATION, CHRONIC-ACTIVE,MODERATELY SEVERE -ULCERATION,-MODERATE -ACANTHOSIS / HYPERKERATOSIS,-MODERATE 2153 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 401 ANIMAL NUMBER: C92633 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 830.9 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 13:20 PROSECTOR: TODD ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PARATHYROID (PT), URINARY BLADDER (UB) 2154 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 402 ANIMAL NUMBER: C92634 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/13/99 STUDY DAY OF DEATH: 603 STUDY WEEK OF DEATH: 87 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/13/99 7:22 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MINIMAL PANCREAS (PA) : -INFILTRATION, FAT, INTERSTITIAL,MINIMAL ADRENAL, CORTEX (AC) : -PIGMENT,-MINIMAL TESTIS (TE) : -ATROPHY/DEGENERATION,-SEVERE >NOTE:>BILATERAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MINIMAL 2155 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 403 ANIMAL NUMBER: C92634 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/13/99 STUDY DAY OF DEATH: 603 STUDY WEEK OF DEATH: 87 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/13/99 7:22 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 7 X 6 X 6 MM, RED, GREY, TAN AND SOFT HEART (HT) : -CARDIOMYOPATHY,-MINIMAL THYROID (TY) : -B-ADENOMA, FOLLICULAR CELL,-NEOPLASM PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT ILEUM (IL) : >AUTOLYTIC CECUM (CE) : >AUTOLYTIC RECTUM (RE) : >AUTOLYTIC URINARY BLADDER (UB) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT EPIDIDYMIDES (EP) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 2156 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 404 ANIMAL NUMBER: C92634 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/13/99 STUDY DAY OF DEATH: 603 STUDY WEEK OF DEATH: 87 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/13/99 7:22 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG), GENERAL COMMENT (GC) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), COLON (CO), SKIN (SK), SEMINAL VESICLES (SV), HEMATO NEOPLASIA (HN) 2157 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 405 ANIMAL NUMBER: C92635 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/20/99 STUDY DAY OF DEATH: 549 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: 481.8 GRAMS DATE AND TIME OF NECROPSY: 10/20/99 10:50 PROSECTOR: JARED FISHEL RECORDER: KEVIN BILLINGS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; RED HAIR COAT; HEADENTIRE; VERIFIED AT NECROPSY: NOT OBSERVED >MASS(ES); HEAD-MAXILLARY-RIGHT; WEEK 76; 20 X 15MM; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >EXCRETION; NONFORMED FECES; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) HISTOPATHOLOGY EYE (EY) : -DYSTROPHY, CORNEAL IN EARLY STAGES, CONSISTS PRIMARILY OF FOCAL MINERALIZATION,-MINIMAL BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-SLIGHT -DEGENERATION, CYSTIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, PELVIC,-SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MINIMAL PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-MODERATE -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL 2158 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 406 ANIMAL NUMBER: C92635 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/20/99 STUDY DAY OF DEATH: 549 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: 481.8 GRAMS DATE AND TIME OF NECROPSY: 10/20/99 10:50 PROSECTOR: JARED FISHEL RECORDER: KEVIN BILLINGS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY TESTIS (TE) : -CONTAINS FLUID; RIGHT: CLEAR AND SOFT MUSCLE, SKELETAL (SM) : -MASS(ES); RIGHT LATERAL MANDIBLE, NEAR BASE OF PINNA: SINGLE MASS, 17 MM IN DIAMETER, TAN AND FIRM; ON SECTION EXUDES A TAN, CASEOUS MATERIAL; COLLECTED INTACT; REGIONAL LN (MANDIBULAR) NORMAL (CAP 22) PITUITARY (PI) : -MASS(ES); 10 MM IN DIAMETER, DARK RED, TAN AND SOFT PROSTATE (PR) : -NODULAR; PROXIMAL SURROUNDING URETHRA: GREEN AND FIRM ADRENAL, CORTEX (AC) : -PIGMENT,-MINIMAL -B-ADENOMA,-NEOPLASM PRESENT TESTIS (TE) : -ATROPHY/DEGENERATION,-SEVERE >NOTE:>UNILATERAL THYMUS (TH) : >SECTION EXAMINED; TISSUE NOT PRESENT HEART (HT) : -CARDIOMYOPATHY,-MINIMAL MUSCLE, SKELETAL (SM) : >UNREMARKABLE >NOTE:>SEE AUDITORY SEBACEOUS GLAND FOR DIAGNOSIS PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE EPIDIDYMIDES (EP) : -HYPOSPERMIA,-PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 2159 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 407 ANIMAL NUMBER: C92635 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/20/99 STUDY DAY OF DEATH: 549 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: 481.8 GRAMS DATE AND TIME OF NECROPSY: 10/20/99 10:50 PROSECTOR: JARED FISHEL RECORDER: KEVIN BILLINGS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY LN, MANDIBULAR (MN) : -HYPERPLASIA, LYMPHOPLASMACYTIC,-SLIGHT AUDITORY SEB GL (AS) : -M-CARCINOMA,-NEOPLASM PRESENT THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG), GENERAL COMMENT (GC) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), HEMATO NEOPLASIA (HN) 2160 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 408 ANIMAL NUMBER: C92636 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/01/00 STUDY DAY OF DEATH: 682 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/01/00 14:29 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; SWOLLEN; TESTES; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; VENTRALABDOMINAL-LEFT; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -MOTTLED; ALL LOBES, DIFFUSE: RED, DARK RED AND TAN LN, MESENTERIC (MS) : -MOTTLED; DIFFUSE: RED, TAN AND GREEN HISTOPATHOLOGY BRAIN (BR) : -MINERALIZATION,-MINIMAL LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -DEGENERATION, CYSTIC,-MINIMAL -CONGESTION,-PRESENT -HYPERPLASIA, BILE DUCT,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE PANCREAS (PA) : -INFILTRATE, EOSINOPHILIC,-MODERATE -FIBROSIS,-SEVERE -INFLAMMATION, GRANULOMATOUS,MODERATELY SEVERE ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-MINIMAL -DEGENERATION, CYSTIC / HEMORRHAGIC,SLIGHT LN, MESENTERIC (MS) : -INFILTRATE, EOSINOPHILIC,-MODERATE -INFLAMMATION, GRANULOMATOUS,-MODERATE 2161 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 409 ANIMAL NUMBER: C92636 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/01/00 STUDY DAY OF DEATH: 682 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/01/00 14:29 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY TESTIS (TE) : -CONTAINS FLUID; BILATERAL: CLEAR FLUID PITUITARY (PI) : -CYST(S); LEFT ASPECT: SINGLE CYST, 2 X 3 MM, FILLED WITH A DARK RED FLUID TESTIS (TE) : -ATROPHY/DEGENERATION,-SEVERE MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,-MODERATE MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,-MODERATE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),SLIGHT -INFLAMMATION, GRANULOMATOUS,-SLIGHT LUNG (LU) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL HEART (HT) : -CARDIOMYOPATHY,-MODERATE PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT ILEUM (IL) : -INFILTRATE, EOSINOPHILIC,-SEVERE -INFLAMMATION, GRANULOMATOUS,-SEVERE COLON (CO) : >AUTOLYTIC PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT EPIDIDYMIDES (EP) : -INFILTRATE, EOSINOPHILIC,-SEVERE 2162 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 410 ANIMAL NUMBER: C92636 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/01/00 STUDY DAY OF DEATH: 682 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/01/00 14:29 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY LN, MEDIASTINAL (ML) : -LARGE; UP TO 3 X 6 X 9 MM (CAP 26) -DIFFUSELY RED; DARK RED (CAP 26) LN, AXILLARY (AX) : -LARGE; BILATERAL: EACH IS 3 X 5 X 8 MM (CAP 27) -DIFFUSELY RED; BILATERAL (CAP 27) LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL (CAP 25) LN, LUMBAR/ILIAC (LM) : -LARGE; BILATERAL: EACH IS 5 X 7 X 11 MM (CAP 28) -DIFFUSELY RED; BILATERAL (CAP 28) PREPUTIAL GLAND (PG) : -LARGE; LEFT: 10 X 10 X 15 MM (RIGHT IS 3 X 5 X 9 MM); LEFT IS FILLED WITH A LIGHT GREEN SEMIFLUID; OVERLYING SKIN HAS A SINGLE DRY, BROWN CRUSTED AREA, 4 X 6 MM (CAP 23) ADIPOSE TISSUE (AT) : -THICKENED; ATTACHED TO BOTH VAS DEFERENS AND EPIDIDYMIDES: FIRM, RED, TAN AND FIBROUS (CAP 24) CAUSE OF DEATH (CD) : -INFLAMMATION / INFECTION,-PRESENT LN, MEDIASTINAL (ML) : -HEMORRHAGE,-PRESENT -INFLAMMATION, GRANULOMATOUS,-SEVERE -INFILTRATE, EOSINOPHILIC,-MODERATE -FIBROSIS,-MODERATE LN, AXILLARY (AX) : -HEMORRHAGE,-PRESENT -HYPERPLASIA, LYMPHOPLASMACYTIC,MODERATE LN, MANDIBULAR (MN) : -HYPERPLASIA, LYMPHOPLASMACYTIC,MODERATELY SEVERE LN, LUMBAR/ILIAC (LM) : -LYMPHANGIECTASIS,-PRESENT -HEMORRHAGE,-PRESENT -INFLAMMATION, SUBACUTE,-SLIGHT PREPUTIAL GLAND (PG) : -INFLAMMATION, CHRONIC-ACTIVE,MODERATELY SEVERE -ECTASIA, DUCTAL,-PRESENT ADIPOSE TISSUE (AT) : -INFILTRATE, EOSINOPHILIC,-MODERATE -INFLAMMATION, GRANULOMATOUS,MODERATELY SEVERE 2163 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 411 ANIMAL NUMBER: C92636 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/01/00 STUDY DAY OF DEATH: 682 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/01/00 14:29 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY MUSCLE, OTHER (MO) : -THICKENED; MUSCLE ASSOCIATED WITH SCROTUM: UP TO 4 MM THICK (CAP 22) -DIFFUSELY RED; MUSCLE ASSOCIATED WITH SCROTUM: DARK RED (CAP 22) MUSCLE, OTHER (MO) : -INFLAMMATION, GRANULOMATOUS,MODERATELY SEVERE -INFILTRATE, EOSINOPHILIC,-MODERATE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG), GENERAL COMMENT (GC) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), CECUM (CE), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), HEMATO NEOPLASIA (HN) 2164 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 412 ANIMAL NUMBER: C92637 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 482.3 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 9:41 PROSECTOR: GREGORY CODDINGTON RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-MINIMAL -NECROSIS, INDIVIDUAL HEPATOCYTE,MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-SLIGHT -FIBROSIS, PERIBILIARY,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MODERATELY SEVERE PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-MODERATE -ATROPHY, ACINAR,-MINIMAL -INFILTRATION, FAT, INTERSTITIAL,MINIMAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT -HYPERTROPHY, FOLLICULAR CELL,MODERATELY SEVERE 2165 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 413 ANIMAL NUMBER: C92637 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 482.3 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 9:41 PROSECTOR: GREGORY CODDINGTON RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -RAISED AREA(S); MID ANTERIOR ASPECT: SINGLE TAN AREA, 1 MM IN DIAMETER GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAr /i LIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: TESTIS (TE), LUNG (LU), PARATHYROID (PT), URINARY BLADDER (UB) 2166 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 414 ANIMAL NUMBER: C92638 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/10/00 STUDY DAY OF DEATH: 631 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: 510.5 GRAMS DATE AND TIME OF NECROPSY: 01/10/00 14:08 PROSECTOR: JAMES T. CALLEN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; PALE; ENTIRE BODY; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) KIDNEY (KD) : -ROUGH SURFACE, DIFFUSE; BILATERAL -DIFFUSELY LIGHT; BILATERAL: TAN PANCREAS (PA) : -LARGE VESSELS; DIFFUSE: UP TO 5 MM IN DIAMETER (CAP 23) HISTOPATHOLOGY BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -DEGENERATION, CYSTIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, PERIPORTAL,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SEVERE -DILATATION, PELVIC,-SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-MODERATE -INFLAMMATION, VASCULAR,-MODERATE ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATE -PIGMENT,-SLIGHT -FIBROSIS,-SLIGHT 2167 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 415 ANIMAL NUMBER: C92638 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/10/00 STUDY DAY OF DEATH: 631 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: 510.5 GRAMS DATE AND TIME OF NECROPSY: 01/10/00 14:08 PROSECTOR: JAMES T. CALLEN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 11 X 9 X 7 MM; RED, TAN, VASCULAR AND FIRM PROSTATE (PR) : -NODULAR; TISSUE SURROUNDING URETHRA: GREEN AND FIRM SEMINAL VESICLES (SV) : -LARGE; BILATERAL: EACH IS 34 X 14 X 6 MM -GELATINOUS; BILATERAL: CLEAR AND SOFT ADRENAL, MEDULLA (MA) : -HYPERPLASIA, MEDULLARY,-MODERATELY SEVERE -B-PHEOCHROMOCYTOMA,-NEOPLASM PRESENT TESTIS (TE) : -INFLAMMATION, VASCULAR,-MODERATELY SEVERE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATELY SEVERE LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL HEART (HT) : -CARDIOMYOPATHY,-SLIGHT PARATHYROID (PT) : -HYPERPLASIA,-MODERATE PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT PROSTATE (PR) : >UNREMARKABLE SEMINAL VESICLES (SV) : -SECRETION, INCREASED,-PRESENT 2168 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 416 ANIMAL NUMBER: C92638 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/10/00 STUDY DAY OF DEATH: 631 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: 510.5 GRAMS DATE AND TIME OF NECROPSY: 01/10/00 14:08 PROSECTOR: JAMES T. CALLEN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY LN, RENAL (RL) : -LARGE; BILATERAL: EACH IS 10 X 6 X 6 MM (CAP 22) -DIFFUSELY RED; BILATERAL: DARK RED AORTA (AO) : -LARGE; UP TO 5 MM IN DIAMETER; COLLECTED -FIRM; ALONG ENTIRE LENGTH GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN EPIDIDYMIDES (EP) : -VACUOLATION, TUBULAR EPITHELIUM,MODERATE CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT LN, RENAL (RL) : -HEMORRHAGE,-PRESENT -DILATATION, SINUSOIDAL,-SEVERE AORTA (AO) : >UNREMARKABLE 2169 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 417 ANIMAL NUMBER: C92638 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/10/00 STUDY DAY OF DEATH: 631 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: 510.5 GRAMS DATE AND TIME OF NECROPSY: 01/10/00 14:08 PROSECTOR: JAMES T. CALLEN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), SPLEEN (SP), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAr /i LIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), CALVARIUm /s KULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), HEMATO NEOPLASIA (HN) 2170 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 418 ANIMAL NUMBER: C92639 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/29/99 STUDY DAY OF DEATH: 589 STUDY WEEK OF DEATH: 85 TERMINAL BODY WEIGHT: 486.0 GRAMS DATE AND TIME OF NECROPSY: 11/29/99 15:10 PROSECTOR: JODI THIEREN RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; HUNCHED; VERIFIED AT NECROPSY: NOT APPLICABLE >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) HISTOPATHOLOGY BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT -HEMORRHAGE,-MINIMAL LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, PELVIC,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-MODERATE -INFILTRATE, MACROPHAGE, PIGMENTED,SLIGHT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATE PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT 2171 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 419 ANIMAL NUMBER: C92639 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/29/99 STUDY DAY OF DEATH: 589 STUDY WEEK OF DEATH: 85 TERMINAL BODY WEIGHT: 486.0 GRAMS DATE AND TIME OF NECROPSY: 11/29/99 15:10 PROSECTOR: JODI THIEREN RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); SINGLE MASS, 14 X 8 X 7 MM, RED, DARK RED, GRAY, TAN, LOBULATED AND FIRM GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN JEJUNUM (JE) : >TISSUE MISSING PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 2172 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 420 ANIMAL NUMBER: C92639 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/29/99 STUDY DAY OF DEATH: 589 STUDY WEEK OF DEATH: 85 TERMINAL BODY WEIGHT: 486.0 GRAMS DATE AND TIME OF NECROPSY: 11/29/99 15:10 PROSECTOR: JODI THIEREN RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2173 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 421 ANIMAL NUMBER: C92640 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 388.9 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 13:27 PROSECTOR: JOHN S. HALFORD RECORDER: MATTHEW AXTMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: MATTHEW AXTMAN ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT' (%) LF ADRENAL (AD0) RT ADRENAL (AD1) BRAIN (BR) LF KIDNEY (KD0) RT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LF TESTIS (TE0) RT TESTIS (TE1) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) .0426 .0356 2.4406 1.9384 1.9241 12.6645 1.8519 .8027 1.7129 1.6810 .0298 .0134 .0110 % .0092 % .6276 % .4984 % .4948 % 3.2565 % .4762 % .2064 % .4404 % .4322 % .0077 % .0034 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0175 .0146 1.0000 .7942 .7884 5.1891 .7588 .3289 .7018 .6888 .0122 .0055 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL KIDNEY (KD) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MINIMAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2174 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 422 ANIMAL NUMBER: C92640 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 388.9 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 13:27 PROSECTOR: JOHN S. HALFORD RECORDER: MATTHEW AXTMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: MATTHEW AXTMAN THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE) 2175 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 423 ANIMAL NUMBER: C92641 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 613.4 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 13:05 PROSECTOR: GREGORY CODDINGTON RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; MISSING; TEETH; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE DARK RED AREAS, UP TO 5 MM IN DIAMETER; COLLECTED ON ROUTINE SECTIONS PITUITARY (PI) : -MASS(ES); 9 X 7 X 6 MM, RED, DARK RED, SOFT HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -MINERALIZATION,-MINIMAL LIVER (LI) : -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-MINIMAL -ANGIECTASIS,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SLIGHT -MINERALIZATION, PELVIC,-SLIGHT -INFLAMMATION, CHRONIC-ACTIVE, PELVIC,MODERATE PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,MODERATELY SEVERE -INFILTRATION, FAT, INTERSTITIAL,MODERATELY SEVERE PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT URINARY BLADDER (UB) : -INFILTRATE, LYMPHOHISTIOCYTIC,MODERATE 2176 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 424 ANIMAL NUMBER: C92641 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 613.4 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 13:05 PROSECTOR: GREGORY CODDINGTON RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -MALOCCLUSION; BILATERAL UPPER INCISORS -TEETH MISSING/BROKEN/WORN; BILATERAL UPPER INCISORS: BROKEN OFF AT BASE URINARY BLADDER (UB) : -HYPERPLASIA, TRANSITIONAL CELL,-SLIGHT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2177 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 425 ANIMAL NUMBER: C92641 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 613.4 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 13:05 PROSECTOR: GREGORY CODDINGTON RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUm /s KULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: TESTIS (TE), LUNG (LU), THYROID (TY), PARATHYROID (PT) 2178 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 426 ANIMAL NUMBER: C92642 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 799.6 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 13:32 PROSECTOR: JERALD W. JOHNSON RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -RED FOCUS(I)/AREA(S); LEFT LATERAL LOBE: MULTIPLE DARK RED AREAS, UP TO 2 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION PANCREAS (PA) : -MASS(ES); NEAR DUODENUM: SINGLE MASS, 8 MM IN DIAMETER, RED, TAN, AND FIRM; SAME ON CUT SURFACE (CAP 22); REGIONAL LN (SEE PANCREATIC) HISTOPATHOLOGY BRAIN (BR) : >UNREMARKABLE LIVER (LI) : -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MODERATE -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL -DEGENERATION, CYSTIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-MODERATE -FIBROSIS, PERIBILIARY,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SLIGHT PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL -M-CARCINOMA, ISLET CELL,-NEOPLASM PRESENT -INFILTRATION, FAT, INTERSTITIAL,SLIGHT LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,SLIGHT 2179 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 427 ANIMAL NUMBER: C92642 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 799.6 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 13:32 PROSECTOR: JERALD W. JOHNSON RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -LARGE; 5 X 4 X 3 MM; APPEARS SWOLLEN -DARK FOCUS(I)/AREA(S); LEFT POSTERIOR ASPECT: SINGLE DARK BROWN AREA, 3 X 2 MM PREPUTIAL GLAND (PG) : -LARGE; RIGHT: 8 X 7 X 7 MM (LEFT IS 8 X 6 X 2 MM); RIGHT CONTAINS A GREEN SEMISOLID (CAP 24) LN, ANT MES/PANC (AP) : -DIFFUSELY RED; DARK RED (CAP 23) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -ANIMAL OBESE THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PITUITARY (PI) : -HYPERPLASIA,-SLIGHT -CYST,-PRESENT URINARY BLADDER (UB) : -PROTEINACEOUS MATERIAL, LUMINAL,PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT PREPUTIAL GLAND (PG) : -INFLAMMATION, CHRONIC-ACTIVE,MODERATELY SEVERE -ECTASIA, DUCTAL,-PRESENT LN, ANT MES/PANC (AP) : -HEMORRHAGE,-PRESENT -HYPERPLASIA, LYMPHOPLASMACYTIC,-SLIGHT -INFILTRATE, MACROPHAGE, PIGMENTED,SLIGHT 2180 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 428 ANIMAL NUMBER: C92642 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 799.6 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 13:32 PROSECTOR: JERALD W. JOHNSON RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), c a l v a r i u m /s k u l l (c a ), j o i n t , o t h e r (j t ), u r e t e r (u e ), a d i p o s e t i s s u e (a t ), d i a p h r a g m (d p ), c a v i t y , t h o r a c i c (t a ), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: TESTIS (TE), PARATHYROID (PT) 2181 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 429 ANIMAL NUMBER: C92643 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 714.4 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 9:47 PROSECTOR: TODD ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); VENTRAL-THORACIC-RIGHT; WEEK 101; 60 X 60MM; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY PITUITARY (PI) : -RED FOCUS(I)/AREA(S); DIFFUSE: MULTIPLE DARK RED AREAS, UP TO 3 X 1 MM HISTOPATHOLOGY LIVER (LI) : -VACUOLATION, HEPATOCELLULAR, MIDZONAL / CENTRILOBULAR,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-SEVERE -DEGENERATION, CYSTIC,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SLIGHT PANCREAS (PA) : -INFILTRATION, FAT, INTERSTITIAL,MODERATE LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERPLASIA, TYPE II PNEUMOCYTE,MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-SLIGHT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT SKIN (SK) : -B-FIBROMA,-NEOPLASM PRESENT 2182 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 430 ANIMAL NUMBER: C92643 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 714.4 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 9:47 PROSECTOR: TODD ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY MAMMARY, MALE (MM) : -MASS(ES); RIGHT AXILLARY REGION: SINGLE MASS, 80 X 65 X 30 MM, RED, DARK RED, TAN, AND FIRM; SAME ON CUT SURFACE; PORTION COLLECTED; REGIONAL LN (AXILLARY) RIGHT NOT IDENTIFIED, LEFT IS NORMAL (CAP 22) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, AXILLARY (AX) : -HEMORRHAGE,-PRESENT -HYPERPLASIA, LYMPHOPLASMACYTIC,MODERATE MAMMARY, MALE (MM) : >NOT REQUIRED TO BE EXAMINED FOR ANIMAL >NOTE:>SEE SKIN FOR DIAGNOSIS OF MASS 2183 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 431 ANIMAL NUMBER: C92643 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 714.4 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 9:47 PROSECTOR: TODD ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: TESTIS (TE), PARATHYROID (PT), URINARY BLADDER (UB) 2184 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 432 ANIMAL NUMBER: C92644 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 658.3 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 13:01 PROSECTOR: TONY HANFELD RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); DORSAL; WEEK 104; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY KIDNEY (KD) : -CYST(S); LEFT WITHIN CORTEX: SINGLE CLEAR FLUID FILLED CYST, 4 X 5 MM PITUITARY (PI) : -RED FOCUS(I)/AREA(S); RIGHT ANTERIOR ASPECT: SINGLE DARK RED AREA, 3 X 1 MM SKIN (SK) : -RAISED FOCUS(I)/AREA(S); LUMBAR REGION: SINGLE DARK RED AND TAN RAISED AREA, 10 X 10 X 6 MM (CAP 22); POSSIBLY CONSISTENT WITH MASS (DORSAL) AS NOTED IN CLINICAL OBSERVATIONS HISTOPATHOLOGY LIVER (LI) : -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL -FIBROSIS, PERIBILIARY,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MODERATE -M-RENAL MESENCHYMAL TUMOR,-NEOPLASM PRESENT PANCREAS (PA) : -INFILTRATION, FAT, INTERSTITIAL,MODERATE PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT SKIN (SK) : -B-ADENOMA, BASAL CELL/SQUAMOUS/SEBACEOUS,-NEOPLASM PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT 2185 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 433 ANIMAL NUMBER: C92644 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 658.3 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 13:01 PROSECTOR: TONY HANFELD RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PROSTATE (PR) : -NODULAR; SURROUNDING URETHRA: BROWN, GREEN, AND FIRM GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -GI TRACT-DARK FLUID CONTENT; ILEUM AND CECUM: DARK GREEN SEMIFLUID CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2186 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 434 ANIMAL NUMBER: C92644 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 658.3 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 13:01 PROSECTOR: TONY HANFELD RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUm /s KULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: TESTIS (TE), LUNG (LU), THYROID (TY), PARATHYROID (PT), URINARY BLADDER (UB) 2187 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 435 ANIMAL NUMBER: C92645 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 474.7 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 13:40 PROSECTOR: DONIA MEYER RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT' (%) LF ADRENAL (AD0) RT ADRENAL (AD1) BRAIN (BR) LF KIDNEY (KD0) RT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LF TESTIS (TE0) RT TESTIS (TE1) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) .0336 .0367 2.1979 2.1058 2.1132 16.1865 1.8951 .8506 1.7410 1.7661 .0117 .0131 .0071 % .0077 % .4630 % .4436 % .4452 % 3.4098 % .3992 % .1792 % .3668 % .3720 % .0025 % .0028 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0153 .0167 1.0000 .9581 .9615 7.3645 .8622 .3870 .7921 .8035 .0053 .0060 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-MINIMAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2188 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 436 ANIMAL NUMBER: C92645 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 474.7 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 13:40 PROSECTOR: DONIA MEYER RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE) 2189 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 437 ANIMAL NUMBER: C92646 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 341.6 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 11:56 PROSECTOR: JODI THIEREN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN STATUS LF ADRENAL (AD0) RT ADRENAL (AD1) BRAIN (BR) LF KIDNEY (KD0) RT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LF TESTIS (TE0) RT TESTIS (TE1) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) -------- 12.3520 --------------- -------- 3.6159 % --------------- -------- ---------------------- NOT TAKEN WEIGHT TAKEN NOT TAKEN NOT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS NOTTAK NOTTAK NOTTAK NOTTAK NOTTAK NOTTAK NOTTAK NOTTAK 2190 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 438 ANIMAL NUMBER: C92646 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 341.6 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 11:56 PROSECTOR: JODI THIEREN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) 2191 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 439 ANIMAL NUMBER: C92647 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/28/99 STUDY DAY OF DEATH: 557 STUDY WEEK OF DEATH: 80 TERMINAL BODY WEIGHT: 558.5 GRAMS DATE AND TIME OF NECROPSY: 10/28/99 9:52 PROSECTOR: JARED FISHEL RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; ROUGH HAIR COAT; VERIFIED AT NECROPSY: NOT OBSERVED >EXCRETION; NO FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >EYES; SQUINTED; PERI-ORBITAL; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) TESTIS (TE) : -SMALL; BILATERAL: EACH IS 18 X 11 X 6 MM; BOTH ARE SOFT HISTOPATHOLOGY BRAIN (BR) : >UNREMARKABLE LIVER (LI) : -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-MODERATE -HYPERPLASIA, BILE DUCT,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT PANCREAS (PA) : -INFILTRATION, FAT, INTERSTITIAL,MODERATE ADRENAL, CORTEX (AC) : -PIGMENT,-SLIGHT TESTIS (TE) : >UNREMARKABLE MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,-MODERATE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),SLIGHT 2192 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 440 ANIMAL NUMBER: C92647 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/28/99 STUDY DAY OF DEATH: 557 STUDY WEEK OF DEATH: 80 TERMINAL BODY WEIGHT: 558.5 GRAMS DATE AND TIME OF NECROPSY: 10/28/99 9:52 PROSECTOR: JARED FISHEL RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 7 MM IN DIAMETER, DARK RED, TAN, AND SOFT STOMACH, GL (ST) : -EROSION/ULCERATION; MUCOSA: MULTIPLE TAN ERODED AREAS, UP TO 4 MM IN DIAMETER PROSTATE (PR) : -SMALL; 17 X 8 X 4 MM SEMINAL VESICLES (SV) : -SMALL; BILATERAL: EACH IS 13 X 6 X 2 MM GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -STAINS-PERINEUM/PERIANAL; YELLOW AND BROWN MATERIAL MOIST MUSCLE, SKELETAL (SM) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL STOMACH, GL (ST) : -INFLAMMATION, ACUTE,-MINIMAL STOMACH, NONGL (SU) : -ACANTHOSIS / HYPERKERATOSIS,-MODERATE -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT SEMINAL VESICLES (SV) : -ATROPHY,-SEVERE CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 2193 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 441 ANIMAL NUMBER: C92647 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/28/99 STUDY DAY OF DEATH: 557 STUDY WEEK OF DEATH: 80 TERMINAL BODY WEIGHT: 558.5 GRAMS DATE AND TIME OF NECROPSY: 10/28/99 9:52 PROSECTOR: JARED FISHEL RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), BONE, STERNUM SPINAL CORD (SC), LUNG (LU), HEART (HT), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) (SB), 2194 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 442 ANIMAL NUMBER: C92648 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/15/99 STUDY DAY OF DEATH: 605 STUDY WEEK OF DEATH: 87 TERMINAL BODY WEIGHT: 411.7 GRAMS DATE AND TIME OF NECROPSY: 12/15/99 14:08 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -LIGHT FOCUS(I)/AREA(S); VISCERAL SURFACE OF RIGHT MEDIAN LOBE, ALONG MARGIN: SINGLE TAN AREA, 4 X 3 MM; COLLECTED ON ROUTINE SECTION HISTOPATHOLOGY BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-SLIGHT -FIBROSIS, PERIBILIARY,-MINIMAL -VACUOLATION, HEPATOCELLULAR,-MODERATE KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SLIGHT -INFLAMMATION, CHRONIC-ACTIVE, PELVIC,SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SLIGHT PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-MODERATE -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL -INFILTRATION, FAT, INTERSTITIAL,SLIGHT 2195 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 443 ANIMAL NUMBER: C92648 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/15/99 STUDY DAY OF DEATH: 605 STUDY WEEK OF DEATH: 87 TERMINAL BODY WEIGHT: 411.7 GRAMS DATE AND TIME OF NECROPSY: 12/15/99 14:08 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 10 X 10 X 9 MM, DARK RED AND SOFT STOMACH, GL (ST) : -THICKENED WALL; MUCOSA, DIFFUSE STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA: MULTIPLE TAN AND BROWN ERODED AREAS, UP TO 8 X 5 MM -THICKENED WALL; MUCOSA, DIFFUSE PROSTATE (PR) : -LARGE; 30 X 20 X 12 MM -GELATINOUS; CLEAR AND SOFT SEMINAL VESICLES (SV) : -LARGE; BILATERAL: EACH IS 36 X 15 X 11 MM ADRENAL, CORTEX (AC) : -HYPERTROPHY, CORTICAL CELL,-MINIMAL -PIGMENT,-MINIMAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),SEVERE HEART (HT) : -CARDIOMYOPATHY,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL STOMACH, GL (ST) : -HYPERPLASIA, EPITHELIAL, GLANDULAR,MODERATE STOMACH, NONGL (SU) : -ACANTHOSIS / HYPERKERATOSIS,MODERATELY SEVERE -ULCERATION,-MODERATE -INFLAMMATION, CHRONIC-ACTIVE,-SEVERE PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE SEMINAL VESICLES (SV) : -SECRETION, INCREASED,-PRESENT 2196 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 444 ANIMAL NUMBER: C92648 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/15/99 STUDY DAY OF DEATH: 605 STUDY WEEK OF DEATH: 87 TERMINAL BODY WEIGHT: 411.7 GRAMS DATE AND TIME OF NECROPSY: 12/15/99 14:08 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAr /i LIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2197 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 445 ANIMAL NUMBER: C92649 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 682.8 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 13:28 PROSECTOR: TROY ELLENBECKER RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -LIGHT FOCUS(I)/AREA(S); AT JUNCTION OF MEDIAN LOBES: SINGLE TAN AREA, 6 X 4 MM; COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-SEVERE -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL -DEGENERATION, CYSTIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL -LIPIDOSIS, SUBCAPSULAR (TENSION LIPIDOSIS),-MODERATE KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL PANCREAS (PA) : -INFILTRATION, FAT, INTERSTITIAL,MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-SLIGHT URINARY BLADDER (UB) : -PROTEINACEOUS MATERIAL, LUMINAL,PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2198 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 446 ANIMAL NUMBER: C92649 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 682.8 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 13:28 PROSECTOR: TROY ELLENBECKER RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: TESTIS (TE), LUNG (LU), PARATHYROID (PT) 2199 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 447 ANIMAL NUMBER: C92650 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 295.5 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 11:49 PROSECTOR: JODI THIEREN RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN STATUS LF ADRENAL (AD0) RT ADRENAL (AD1) BRAIN (BR) LF KIDNEY (KD0) RT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LF TESTIS (TE0) RT TESTIS (TE1) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) -------- 12.2299 --------------- -------- 4.1391 % --------------- -------- ---------------------- NOT TAKEN WEIGHT TAKEN NOT TAKEN NOT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS NOTTAK NOTTAK NOTTAK NOTTAK NOTTAK NOTTAK NOTTAK NOTTAK 2200 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 448 ANIMAL NUMBER: C92650 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 295.5 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 11:49 PROSECTOR: JODI THIEREN RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) 2201 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 449 ANIMAL NUMBER: C92651 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/01/99 STUDY DAY OF DEATH: 561 STUDY WEEK OF DEATH: 81 TERMINAL BODY WEIGHT: 375.1 GRAMS DATE AND TIME OF NECROPSY: 11/01/99 13:35 PROSECTOR: JOHN S. HALFORD RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; SWOLLEN; PAW-HIND-RIGHT; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; SWOLLEN; TAIL-PROXIMAL; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >EYES; SQUINTED; PERI-ORBITAL; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LN, MESENTERIC (MS) : -SMALL; UP TO 2 MM IN DIAMETER THYMUS (TH) : -MISSING/NOT IDENTIFIED; NOT POSITIVELY IDENTIFIED; POSSIBLE REMNANTS COLLECTED HISTOPATHOLOGY BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -HYPERPLASIA, BILE DUCT,-SLIGHT -FIBROSIS, PERIBILIARY,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-MODERATE -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL -HYPERPLASIA, ISLET CELLS,-MINIMAL ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-MINIMAL -ANGIECTASIS,-MINIMAL LN, MESENTERIC (MS) : >UNREMARKABLE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),SEVERE 2202 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 450 ANIMAL NUMBER: C92651 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/01/99 STUDY DAY OF DEATH: 561 STUDY WEEK OF DEATH: 81 TERMINAL BODY WEIGHT: 375.1 GRAMS DATE AND TIME OF NECROPSY: 11/01/99 13:35 PROSECTOR: JOHN S. HALFORD RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 13 X 11 X 8 MM, RED, DARK RED, GRAY, TAN, LOBULATED, AND SOFT STOMACH, GL (ST) : -EROSION/ULCERATION; MUCOSA: MULTIPLE TAN AND BROWN SLIGHTLY ERODED AREAS, UP TO 3 X 2 MM SEMINAL VESICLES (SV) : -LARGE; BILATERAL: EACH IS 31 X 21 X 6 MM; BOTH APPEAR GELATINOUS TAIL (TI) : -LARGE; AT BASE: UP TO 15 MM IN DIAMETER; APPEARS FIRM AND RIGID; PORTION COLLECTED PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL STOMACH, GL (ST) : >UNREMARKABLE PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT SEMINAL VESICLES (SV) : -SECRETION, INCREASED,-PRESENT EPIDIDYMIDES (EP) : >UNREMARKABLE >NOTE:>VAS DEFERENS - UNREMARKABLE CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT MAMMARY, MALE (MM) : -GALACTOCELE,-PRESENT TAIL (TI) : -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL LN, LUMBAR/ILIAC (LM) : -HYPERPLASIA, LYMPHOPLASMACYTIC,MODERATE 2203 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 451 ANIMAL NUMBER: C92651 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/01/99 STUDY DAY OF DEATH: 561 STUDY WEEK OF DEATH: 81 TERMINAL BODY WEIGHT: 375.1 GRAMS DATE AND TIME OF NECROPSY: 11/01/99 13:35 PROSECTOR: JOHN S. HALFORD RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY CAVITY, ABDOM (PC) : -INFLAMMATION / NECROSIS, FAT,-MODERATE >NOTE:>GROSS RECORDED UNDER ADIPOSE TISSUE JOINT, OTHER (JT) : JOINT, OTHER (JT) : -LARGE; RIGHT, TARSAL: 15 X 11 X 11 MM -INFLAMMATION, CHRONIC-ACTIVE,-SEVERE (LEFT IS 10 X 9 X 8 MM); BOTH COLLECTED ADIPOSE TISSUE (AT) : ADIPOSE TISSUE (AT) : -MASS(ES); TISSUE SURROUNDING LEFT VAS >NOT REQUIRED TO BE EXAMINED FOR ANIMAL DEFERENS: SINGLE FIRM AND YELLOW MASS, 17 X 8 X 3 MM; ON SECTION, EXUDES A YELLOW CASEOUS SEMIFLUID MATERIAL (CAP 22) ; REGIONAL LN (LUMBAR) NORMAL (CAP 23) VAS DEFERENS (VS) : VAS DEFERENS (VS) : -LARGE; BILATERAL: EACH IS UP TO 5 MM >NOT REQUIRED TO BE EXAMINED FOR ANIMAL IN DIAMETER; BOTH APPEAR SOFT AND GELATINOUS (CAP 24) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN 2204 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 452 ANIMAL NUMBER: C92651 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/01/99 STUDY DAY OF DEATH: 561 STUDY WEEK OF DEATH: 81 TERMINAL BODY WEIGHT: 375.1 GRAMS DATE AND TIME OF NECROPSY: 11/01/99 13:35 PROSECTOR: JOHN S. HALFORD RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUm /s KULL (CA), LN, ANT MES/PANC (AP), URETER (UE), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), HEMATO NEOPLASIA (HN) 2205 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 453 ANIMAL NUMBER: C92652 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 292.4 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 11:31 PROSECTOR: LYNN HORNER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN STATUS LF ADRENAL (AD0) RT ADRENAL (AD1) BRAIN (BR) LF KIDNEY (KD0) RT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LF TESTIS (TE0) RT TESTIS (TE1) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) ------------------------------------ 11.5416 ------------------------------------------- ------------------------------------ 3.9468 % ------------------------------------------- ------------------------------------------------------------------------------------- NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN WEIGHT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS 2206 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 454 ANIMAL NUMBER: C92652 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 292.4 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 11:31 PROSECTOR: LYNN HORNER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) 2207 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 455 ANIMAL NUMBER: C92653 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 771.5 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 11:24 PROSECTOR: STEVE VAN ADESTINE RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE DARK RED AREAS, UP TO 3 MM IN DIAMETER; COLLECTED ON ROUTINE SECTIONS KIDNEY (KD) : -ROUGH SURFACE, DIFFUSE; BILATERAL -DIFFUSELY LIGHT; BILATERAL: TAN TESTIS (TE) : -SMALL; LEFT: 17 X 12 X 8 MM (RIGHT IS 22 X 14 X 10 MM) HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -NECROSIS, INDIVIDUAL HEPATOCYTE,MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL -DEGENERATION, CYSTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, PERIPORTAL,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MODERATELY SEVERE PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-MODERATE -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL TESTIS (TE) : -ATROPHY/DEGENERATION,-SLIGHT -INFLAMMATION, VASCULAR,-MODERATE THYROID (TY) : -DILATATION / CYST, FOLLICULAR,-PRESENT -B-ADENOMA, FOLLICULAR CELL,-NEOPLASM PRESENT 2208 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 456 ANIMAL NUMBER: C92653 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 771.5 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 11:24 PROSECTOR: STEVE VAN ADESTINE RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 8 MM IN DIAMETER, RED, GRAY, AND SOFT PROSTATE (PR) : -NODULAR; PROXIMAL SURROUNDING URETHRA: GREEN AND FIRM SEMINAL VESICLES (SV) : -GELATINOUS; BILATERAL: YELLOW AND CLEAR GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL SEMINAL VESICLES (SV) : >UNREMARKABLE CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2209 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 457 ANIMAL NUMBER: C92653 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 771.5 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 11:24 PROSECTOR: STEVE VAN ADESTINE RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PARATHYROID (PT), URINARY BLADDER (UB) 2210 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 458 ANIMAL NUMBER: C92654 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/20/99 STUDY DAY OF DEATH: 549 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: 517.1 GRAMS DATE AND TIME OF NECROPSY: 10/20/99 10:12 PROSECTOR: JARED FISHEL RECORDER: KEVIN BILLINGS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >EYES; OPAQUE; EYE-LEFT; VERIFIED AT NECROPSY: OBSERVED >EYES; SCAB; EYE-LEFT; VERIFIED AT NECROPSY: NOT OBSERVED >EYES; PROTRUDING; EYE-LEFT; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; NO FECES; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY EYE (EY) : -OPAQUE CORNEA; LEFT -PROTRUDING; LEFT -LARGE; LEFT: 9 MM IN DIAMETER IS 6 MM IN DIAMETER) (RIGHT HISTOPATHOLOGY EYE (EY) : -I-NEUROFIBROSARCOMA,-NEOPLASM PRESENT BRAIN (BR) : -M-NEUROFIBROSARCOMA,-NEOPLASM PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -NECROSIS, INDIVIDUAL HEPATOCYTE,MINIMAL -DEGENERATION, CYSTIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL -VACUOLATION, HEPATOCELLULAR,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -DILATATION, PELVIC,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MINIMAL PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL ADRENAL, CORTEX (AC) : -HYPERTROPHY, CORTICAL CELL,-MINIMAL 2211 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 459 ANIMAL NUMBER: C92654 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/20/99 STUDY DAY OF DEATH: 549 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: 517.1 GRAMS DATE AND TIME OF NECROPSY: 10/20/99 10:12 PROSECTOR: JARED FISHEL RECORDER: KEVIN BILLINGS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY HARDERIAN GLAND (HG) : -MISSING/NOT IDENTIFIED; BILATERAL: NOT POSITIVELY IDENTIFIED DUE TO CALVARIUM MASS; POSSIBLE REMNANTS COLLECTED LN, MANDIBULAR (MN) : -LARGE; LEFT: UP TO 12 X 8 X 4 MM (RIGHT IS UP TO 6 X 4 X 2 MM); COLLECTED ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC / HEMORRHAGIC,MINIMAL MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,-SLIGHT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATELY SEVERE PITUITARY (PI) : -HYPERPLASIA,-SLIGHT -CYST,-PRESENT HARDERIAN GLAND (HG) : >TISSUE MISSING STOMACH, NONGL (SU) : -ACANTHOSIS / HYPERKERATOSIS,-MODERATE -ULCERATION,-MINIMAL -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL CAUSE OF DEATH (CD) : -NEOPLASM, BRAIN,-PRESENT LN, MANDIBULAR (MN) : -HYPERPLASIA, LYMPHOPLASMACYTIC,MODERATE -LYMPHANGIECTASIS,-PRESENT 2212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 460 ANIMAL NUMBER: C92654 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/20/99 STUDY DAY OF DEATH: 549 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: 517.1 GRAMS DATE AND TIME OF NECROPSY: 10/20/99 10:12 PROSECTOR: JARED FISHEL RECORDER: KEVIN BILLINGS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY CALVARIUM/SKULL (CA) : -MASS(ES); BILATERAL ORBITS, EXTENDING INTO CRANIAL VAULT: SINGLE MASS, TAN AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; LEFT: DARK RED AND DRY MATERIAL CALVARIUM/SKULL (CA) : -I-SARCOMA,-NEOPLASM PRESENT 2213 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 461 ANIMAL NUMBER: C92654 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/20/99 STUDY DAY OF DEATH: 549 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: 517.1 GRAMS DATE AND TIME OF NECROPSY: 10/20/99 10:12 PROSECTOR: JARED FISHEL RECORDER: KEVIN BILLINGS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) (UB), 2214 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 462 ANIMAL NUMBER: C92655 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/01/00 STUDY DAY OF DEATH: 653 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: 669.9 GRAMS DATE AND TIME OF NECROPSY: 02/01/00 11:19 PROSECTOR: STEVE VAN ADESTINE RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); TAIL-MID; WEEK 85; 20 X 25MM; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; TAIL-MID; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -DIFFUSELY LIGHT; ALL LOBES: TAN HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -DEGENERATION, CYSTIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MODERATE SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : -PIGMENT,-MINIMAL ADRENAL, MEDULLA (MA) : -HYPERPLASIA, MEDULLARY,-SLIGHT MARROW, FEMUR (FM) : -HYPERPLASIA, ERYTHROID, MARROW,MODERATE MARROW, STERNUM (SE) : -HYPERPLASIA, ERYTHROID, MARROW,MODERATE 2215 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 463 ANIMAL NUMBER: C92655 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/01/00 STUDY DAY OF DEATH: 653 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: 669.9 GRAMS DATE AND TIME OF NECROPSY: 02/01/00 11:19 PROSECTOR: STEVE VAN ADESTINE RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 7 X 5 X 5 MM, RED, DARK RED, TAN, LOBULATED, AND SOFT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATE LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL -INFLAMMATION, GRANULOMATOUS,-MINIMAL HEART (HT) : -CARDIOMYOPATHY,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : -HYPERPLASIA, EPITHELIAL, GLANDULAR,MODERATE PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT CAUSE OF DEATH (CD) : -NEOPLASM, SKIN/MUSCLE/SUBCUTIS,PRESENT TAIL (TI) : -INFLAMMATION, CHRONIC-ACTIVE,MODERATELY SEVERE -ULCERATION,-MODERATELY SEVERE -M-NEUROFIBROSARCOMA,-NEOPLASM PRESENT 2216 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 464 ANIMAL NUMBER: C92655 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/01/00 STUDY DAY OF DEATH: 653 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: 669.9 GRAMS DATE AND TIME OF NECROPSY: 02/01/00 11:19 PROSECTOR: STEVE VAN ADESTINE RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY TAIL (TI) : -MASS(ES); 35 MM FROM BASE: SINGLE MASS, 35 X 32 X 20 MM, RED, DARK RED, TAN, AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (LUMBAR) NORMAL (CAP 22); OVERLYING SKIN HAS A SINGLE, DARK RED ULCERATED AREA, 20 MM IN DIAMETER; COLLECTED INTACT WITH MASS GENERAL COMMENT (GC) : -BLOOD FILM TAKEN LN, LUMBAR/ILIAC (LM) : -HYPERPLASIA, LYMPHOPLASMACYTIC,-SLIGHT 2217 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 465 ANIMAL NUMBER: C92655 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/01/00 STUDY DAY OF DEATH: 653 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: 669.9 GRAMS DATE AND TIME OF NECROPSY: 02/01/00 11:19 PROSECTOR: STEVE VAN ADESTINE RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2218 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 466 ANIMAL NUMBER: C92656 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/21/00 STUDY DAY OF DEATH: 673 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 462.2 GRAMS DATE AND TIME OF NECROPSY: 02/21/00 13:57 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; SORE/SCAB; DORSALTHORACIC; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; PALE; ENTIRE BODY; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) KIDNEY (KD) : -CYST(S); BILATERAL, CORTICAL SURFACE: MULTIPLE PINPOINT, CLEAR FLUID FILLED CYSTS -ROUGH SURFACE, DIFFUSE; BILATERAL -DIFFUSELY LIGHT; BILATERAL: TAN HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -DEGENERATION, CYSTIC,-MINIMAL -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL -FIBROSIS, PERIBILIARY,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SEVERE -INFLAMMATION, SUPPURATIVE, PELVIC,MINIMAL -THROMBUS,-PRESENT SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,MODERATELY SEVERE -ATROPHY, ACINAR,-MINIMAL -INFLAMMATION, VASCULAR,-MODERATE ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-MINIMAL -HYPERPLASIA, CORTICAL,-MINIMAL 2219 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 467 ANIMAL NUMBER: C92656 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/21/00 STUDY DAY OF DEATH: 673 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 462.2 GRAMS DATE AND TIME OF NECROPSY: 02/21/00 13:57 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY TESTIS (TE) : -SMALL; BILATERAL: EACH IS 20 X 10 X 5 MM HEART (HT) : -LIGHT FOCUS(I)/AREA(S); EPICARDIUM OF BOTH VENTRICLES: MULTIPLE TAN, INDISTINCT AREAS PARATHYROID (PT) : -LARGE; BILATERAL: EACH IS 2 MM IN DIAMETER ADRENAL, MEDULLA (MA) : -HYPERPLASIA, MEDULLARY,-SLIGHT LN, MESENTERIC (MS) : -MINERALIZATION, VASCULAR,-SLIGHT TESTIS (TE) : -ATROPHY/DEGENERATION,-MINIMAL -INFLAMMATION, VASCULAR,-SLIGHT BONE, FEMUR (FE) : -OSTEODYSTROPHY, FIBROUS,-SLIGHT BONE, STERNUM (SB) : -OSTEODYSTROPHY, FIBROUS,-MINIMAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY) MODERATELY SEVERE LUNG (LU) : -MINERALIZATION, SEPTAL,-SLIGHT HEART (HT) : -CARDIOMYOPATHY,-MODERATELY SEVERE -MINERALIZATION, VASCULAR,-SEVERE THYROID (TY) : -B-ADENOMA, FOLLICULAR CELL,-NEOPLASM PRESENT PARATHYROID (PT) : -HYPERPLASIA,-SEVERE PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT 2220 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 468 ANIMAL NUMBER: C92656 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/21/00 STUDY DAY OF DEATH: 673 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 462.2 GRAMS DATE AND TIME OF NECROPSY: 02/21/00 13:57 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 10 X 8 X 8 MM, RED, GRAY, TAN, AND SOFT STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: MULTIPLE BROWN AREAS, UP TO 3 X 2 MM -THICKENED WALL; DIFFUSE SKIN (SK) : -CRUSTED AREA(S); LEFT DORSAL THORACIC REGION: SINGLE TAN, LOBULATED CRUSTED AREA, 18 X 10 MM (CAP 25) PROSTATE (PR) : -NODULAR; DIFFUSE: GREEN AND FIRM MAMMARY, MALE (MM) : -CYST(S); ALL REGIONS, DIFFUSE: MULTIPLE TAN, CREAMY SEMIFLUID FILLED CYSTS, UP TO 5 MM IN DIAMETER (CAP 22) AORTA (AO) : -LARGE; DIFFUSE: UP TO 8 MM IN DIAMETER; COLLECTED -FIRM; DIFFUSE STOMACH, GL (ST) : -MINERALIZATION, GLANDULAR MUCOSA,SLIGHT SKIN (SK) : -B-KERATOACANTHOMA,-NEOPLASM PRESENT URINARY BLADDER (UB) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SEVERE SEMINAL VESICLES (SV) : -SECRETION, DECREASED,-PRESENT EPIDIDYMIDES (EP) : -IMMATURE SPERM FORMS,-MINIMAL CAUSE OF DEATH (CD) : -NEPHROPATHY,-PRESENT MAMMARY, MALE (MM) : -HYPERPLASIA, CYSTIC,-MODERATE -GALACTOCELE,-PRESENT AORTA (AO) : -MINERALIZATION,-MODERATELY SEVERE 2221 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 469 ANIMAL NUMBER: C92656 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/21/00 STUDY DAY OF DEATH: 673 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 462.2 GRAMS DATE AND TIME OF NECROPSY: 02/21/00 13:57 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY ARTERY, OTHER (AU) : -LARGE; BILATERAL, CAROTID: EACH IS UP TO 3 MM IN DIAMETER AND BOTH ARE FIRM (CAP 23) AORTIC ARCH (AA) : -LARGE; UP TO 10 MM IN DIAMETER AND FIRM (CAP 24) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN ARTERY, OTHER (AU) : -MINERALIZATION,-MODERATELY SEVERE AORTIC ARCH (AA) : -MINERALIZATION,-MODERATELY SEVERE 2222 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 470 ANIMAL NUMBER: C92656 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/21/00 STUDY DAY OF DEATH: 673 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 462.2 GRAMS DATE AND TIME OF NECROPSY: 02/21/00 13:57 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), MARROW, FEMUR (FM), MARROW, STERNUM (SE), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), HEMATO NEOPLASIA (HN) 2223 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 471 ANIMAL NUMBER: C92657 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 785.8 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 8:39 PROSECTOR: DAVID SCHUETTE RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >WART LIKE LESION(S); SHOULDER-LEFT; WEEK 90; VERIFIED AT NECROPSY: NOT OBSERVED OBSERVATIONS NECROPSY PITUITARY (PI) : -RED FOCUS(I)/AREA(S); LEFT ASPECT: SINGLE DARK RED AREA, 3 MM IN DIAMETER HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SLIGHT -MINERALIZATION, PELVIC,-MODERATE PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL -INFILTRATION, FAT, INTERSTITIAL,SLIGHT LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL THYROID (TY) : -DILATATION / CYST, FOLLICULAR,-PRESENT PITUITARY (PI) : -HYPERPLASIA,-MODERATE SKIN (SK) : -B-KERATOACANTHOMA,-NEOPLASM PRESENT 2224 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 472 ANIMAL NUMBER: C92657 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 785.8 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 8:39 PROSECTOR: DAVID SCHUETTE RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY SKIN (SK) : -MASS(ES); LEFT LATERAL THORACIC REGION: SINGLE MASS, 12 X 12 X 10 MM TAN AND HARD; SAME ON CUT SURFACE; OVERLYING SKIN HAS A SINGLE DRY, TAN CRUSTED, ULCERATED AREA, 10 MM IN DIAMETER (CAP 22); REGIONAL LN (AXILLARY) NORMAL (CAP 23) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, AXILLARY (AX) : -HYPERPLASIA, LYMPHOPLASMACYTIC,-SLIGHT 2225 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 473 ANIMAL NUMBER: C92657 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 785.8 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 8:39 PROSECTOR: DAVID SCHUETTE RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAr /i LIAC (LM), CAVITY, ABDOM (PC), AUDITORYSEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: TESTIS (TE), PARATHYROID (PT), URINARY BLADDER (UB) 2226 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 474 ANIMAL NUMBER: C92658 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 02/01/00 STUDY DAY OF DEATH: 653 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 02/01/00 14:40 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >EYES; RED DISCHARGE; EYE-RIGHT; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) HISTOPATHOLOGY BRAIN (BR) : ^UNREMARKABLE LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -DEGENERATION, CYSTIC,-SLIGHT -HYPERPLASIA, BILE DUCT,-SLIGHT -FIBROSIS, PERIBILIARY,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,MODERATELY SEVERE -ATROPHY, ACINAR,-MINIMAL ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-SLIGHT -PIGMENT,-MINIMAL -NECROSIS, FOCAL,-MINIMAL THYMUS (TH) : >SECTION EXAMINED; TISSUE NOT PRESENT 2227 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 475 ANIMAL NUMBER: C92658 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 02/01/00 STUDY DAY OF DEATH: 653 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 02/01/00 14:40 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 6 X 6 X 3 MM, RED, DARK RED, GREY AND SOFT GENERAL COMMENT (GC) : -DISCHARGE-OCULAR; BILATERAL: LEFT IS RED, RIGHT IS RED AND BROWN -ANIMAL OBESE HEART (HT) : -CARDIOMYOPATHY,-MINIMAL MUSCLE, SKELETAL (SM) : -ATROPHY,-SLIGHT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT ILEUM (IL) : >AUTOLYTIC CECUM (CE) : >AUTOLYTIC PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL CAUSE OF DEATH (CD) : -UNDETERMINED MICROSCOPICALLY,-PRESENT 2228 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 476 ANIMAL NUMBER: C92658 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 02/01/00 STUDY DAY OF DEATH: 653 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 02/01/00 14:40 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM(RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2229 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 477 ANIMAL NUMBER: C92659 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 705.8 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 10:04 PROSECTOR: TODD ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; SWOLLEN; PAW-HIND-LEFT; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: NOT OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -LIGHT FOCUS(I)/AREA(S); AT BIFURCATION OF RIGHT AND LEFT MEDIAN LOBE: SINGLE TAN AREA, 4 X 3 MM (CAP 22) HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-MINIMAL -VACUOLATION, HEPATOCELLULAR, MIDZONAL / CENTRILOBULAR,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-SLIGHT -DEGENERATION, CYSTIC,-MINIMAL -LIPIDOSIS, SUBCAPSULAR (TENSION LIPIDOSIS),-SEVERE KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, PELVIC,-SLIGHT -HYPERPLASIA, TRANSITIONAL CELL,-SLIGHT PANCREAS (PA) : -B-ADENOMA, ISLET CELL,-NEOPLASM PRESENT -INFILTRATION, FAT, INTERSTITIAL,SLIGHT 2230 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 478 ANIMAL NUMBER: C92659 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 705.8 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 10:04 PROSECTOR: TODD ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -RAISED AREA(S); LEFT POSTERIOR ASPECT: SINGLE DARK RED AREA, 2 MM IN DIAMETER JOINT, OTHER (JT) : -LARGE; LEFT TARSAL JOINT: 15 MM IN DIAMETER (RIGHT IS 9 MM IN DIAMETER); COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN TESTIS (TE) : -ATROPHY/DEGENERATION,-SEVERE LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT -HYPERPLASIA,-MODERATE CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT JOINT, OTHER (JT) : -INFLAMMATION, CHRONIC-ACTIVE,MODERATELY SEVERE 2231 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 479 ANIMAL NUMBER: C92659 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 705.8 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 10:04 PROSECTOR: TODD ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: THYROID (TY), PARATHYROID (PT), URINARY BLADDER (UB) 2232 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 480 ANIMAL NUMBER: C92660 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 659.1 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 12:46 PROSECTOR: GREGORY CODDINGTON RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE DARK RED AREAS, UP TO 2 MM IN DIAMETER, COLLECTED ON ROUTINE SECTIONS PITUITARY (PI) : -MASS(ES); 8 X 7 X 5 MM, RED, DARK RED, TAN, AND SOFT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, PERIPORTAL,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SLIGHT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2233 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 481 ANIMAL NUMBER: C92660 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 659.1 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 12:46 PROSECTOR: GREGORY CODDINGTON RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUm /s KULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), TESTIS (TE), LUNG (LU), THYROID (TY), PARATHYROID (PT), URINARY BLADDER (UB) 2234 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 482 ANIMAL NUMBER: C92661 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/03/00 STUDY DAY OF DEATH: 624 STUDY WEEK OF DEATH: 90 TERMINAL BODY WEIGHT: 376.6 GRAMS DATE AND TIME OF NECROPSY: 01/03/00 14:05 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; PALE; ENTIRE BODY; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -MASS(ES); RIGHT LATERAL LOBE: SINGLE PEDUNCULATED MASS, 40 X 20 X 10 MM, DARK RED, BROWN, TAN, AND FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (SEE HEPATIC) -DIFFUSELY LIGHT; ALL LOBES: TAN KIDNEY (KD) : -CYST(S); BILATERAL, DIFFUSE: MULTIPLE CLEAR FLUID FILLED CYSTS, UP TO 3 MM IN DIAMETER HISTOPATHOLOGY EYE (EY) : -DYSTROPHY, CORNEAL IN EARLY STAGES, CONSISTS PRIMARILY OF FOCAL MINERALIZATION,-SLIGHT BRAIN (BR) : -MINERALIZATION,-MINIMAL LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-SLIGHT -VACUOLATION, HEPATOCELLULAR, MIDZONAL / CENTRILOBULAR,-SLIGHT -NECROSIS, COAGULATIVE,-MODERATE -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL -HYPERPLASIA, BILE DUCT,-MODERATE -FIBROSIS, PERIBILIARY,-SLIGHT -HYPERTROPHY, HEPATOCELLULAR, PERIPORTAL,-MODERATE -INFILTRATE, MACROPHAGE, PIGMENTED,MODERATE -HEMORRHAGE,-PRESENT -B-ADENOMA, HEPATOCELLULAR,-NEOPLASM PRESENT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SEVERE 2235 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 483 ANIMAL NUMBER: C92661 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/03/00 STUDY DAY OF DEATH: 624 STUDY WEEK OF DEATH: 90 TERMINAL BODY WEIGHT: 376.6 GRAMS DATE AND TIME OF NECROPSY: 01/03/00 14:05 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY KIDNEY (KD) : -LARGE PELVIS(ES); BILATERAL LUNG (LU) : -DIFFUSELY LIGHT; ALL LOBES: LIGHT RED SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE PANCREAS (PA) : -INFLAMMATION, VASCULAR,-MODERATE ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-MINIMAL -PIGMENT,-MINIMAL ADRENAL, MEDULLA (MA) : -B-PHEOCHROMOCYTOMA,-NEOPLASM PRESENT TESTIS (TE) : -ATROPHY/DEGENERATION,-SLIGHT >NOTE:>UNILATERAL MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,-MODERATE MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,-MODERATE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),SEVERE LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL -MINERALIZATION, SEPTAL,-SLIGHT HEART (HT) : -CARDIOMYOPATHY,-MINIMAL 2236 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 484 ANIMAL NUMBER: C92661 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/03/00 STUDY DAY OF DEATH: 624 STUDY WEEK OF DEATH: 90 TERMINAL BODY WEIGHT: 376.6 GRAMS DATE AND TIME OF NECROPSY: 01/03/00 14:05 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 7 X 6 X 6 MM, TAN, DARK RED, AND SOFT STOMACH, GL (ST) : -RED FOCUS(I)/AREA(S); MUCOSA: MULTIPLE DARK RED PINPOINT FOCI, UP TO 1 MM IN DIAMETER -THICKENED WALL; DIFFUSE LN, OTHER (LN) : -DIFFUSELY RED; DARK RED (CAP 22) AORTA (AO) : -LARGE; UP TO 7 MM IN DIAMETER; COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN HEART (HT) : -MINERALIZATION, VASCULAR,-MODERATE PARATHYROID (PT) : -HYPERPLASIA,-SEVERE PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL STOMACH, GL (ST) : -HYPERPLASIA, EPITHELIAL, GLANDULAR,MODERATE -MINERALIZATION, GLANDULAR MUCOSA,MODERATELY SEVERE PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL CAUSE OF DEATH (CD) : -NEOPLASM, LIVER,-PRESENT LN, OTHER (LN) : -HEMORRHAGE,-PRESENT -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL AORTA (AO) : -MINERALIZATION,-SEVERE 2237 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 485 ANIMAL NUMBER: C92661 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/03/00 STUDY DAY OF DEATH: 624 STUDY WEEK OF DEATH: 90 TERMINAL BODY WEIGHT: 376.6 GRAMS DATE AND TIME OF NECROPSY: 01/03/00 14:05 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LN, MESENTERIC (MS), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2238 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 486 ANIMAL NUMBER: C92662 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 431.4 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 11:50 PROSECTOR: LAURIE J. SCHULLER RECORDER: KIMBERLY DURLAND POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT' (%) LF ADRENAL (AD0) RT ADRENAL (AD1) BRAIN (BR) LF KIDNEY (KD0) RT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LF TESTIS (TE0) RT TESTIS (TE1) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) .0238 .0252 2.2508 1.6677 2.0046 14.3620 1.9229 .6613 1.6859 1.7014 .0084 .0146 .0055 % .0058 % .5217 % .3866 % .4647 % 3.3292 % .4457 % .1533 % .3908 % .3944 % .0019 % .0034 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0106 .0112 1.0000 .7409 .8906 6.3808 .8543 .2938 .7490 .7559 .0037 .0065 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED KIDNEY (KD) : -LARGE PELVIS(ES); RIGHT GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL KIDNEY (KD) : -DILATATION, PELVIC,-SLIGHT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2239 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 487 ANIMAL NUMBER: C92662 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 431.4 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 11:50 PROSECTOR: LAURIE J. SCHULLER RECORDER: KIMBERLY DURLAND POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAr /i LIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE) 2240 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 488 ANIMAL NUMBER: C92663 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 02/08/00 STUDY DAY OF DEATH: 660 STUDY WEEK OF DEATH: 95 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 02/08/00 14:13 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); SHOULDER-LEFT; WEEK 89; 70 X 80MM; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) KIDNEY (KD) : -LARGE PELVIS(ES); RIGHT -GRANULAR MATERIAL; RIGHT: MULTIPLE TAN AND HARD GRANULES, UP TO 1 MM IN DIAMETER PANCREAS (PA) : -MASS(ES); DISTAL END FROM SPLEEN: SINGLE MASS, 7 MM IN DIAMETER, DARK RED AND FIRM; SAME ON CUT SURFACE (CAP 23); REGIONAL LN (PANCREATIC) NOT IDENTIFIED HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MODERATE -HYPERPLASIA, BILE DUCT,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MODERATE -DILATATION, PELVIC,-SLIGHT -MINERALIZATION, PELVIC,-MODERATE SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SLIGHT PANCREAS (PA) : -M-CARCINOMA, ISLET CELL,-NEOPLASM PRESENT ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-MINIMAL 2241 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 489 ANIMAL NUMBER: C92663 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 02/08/00 STUDY DAY OF DEATH: 660 STUDY WEEK OF DEATH: 95 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 02/08/00 14:13 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY TESTIS (TE) : -SMALL; BILATERAL: EACH IS 22 X 13 X 4 MM; BOTH ARE SOFT PITUITARY (PI) : -MASS(ES); 7 X 6 X 5 MM, DARK RED, TAN, GRAY AND SOFT ADRENAL, MEDULLA (MA) : -HYPERPLASIA, MEDULLARY,-MINIMAL TESTIS (TE) : -ATROPHY/DEGENERATION,-SEVERE -GRANULOMA, SPERM,-PRESENT MARROW, FEMUR (FM) : -HYPERPLASIA, ERYTHROID, MARROW,MODERATELY SEVERE MARROW, STERNUM (SE) : -HYPERPLASIA, ERYTHROID, MARROW,MODERATELY SEVERE THYMUS (TH) : -HEMORRHAGE,-PRESENT -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATE HEART (HT) : -CARDIOMYOPATHY,-MINIMAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) -ATROPHY,-MINIMAL JEJUNUM (JE) : >AUTOLYTIC ILEUM (IL) : >AUTOLYTIC : 2242 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 490 ANIMAL NUMBER: C92663 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 02/08/00 STUDY DAY OF DEATH: 660 STUDY WEEK OF DEATH: 95 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 02/08/00 14:13 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY SKIN (SK) : -MASS(ES); LEFT SHOULDER REGION: SINGLE MASS, 80 X 65 X 40 MM, RED, DARK RED, TAN, GRAY, LOBULATED AND FIRM; ON SECTION EXUDES A DARK RED FLUID; PORTION COLLECTED (CAP 25); REGIONAL LN (SEE AXILLARY) LN, AXILLARY (AX) : -DIFFUSELY RED; BILATERAL: DARK RED (CAP 24) LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL: DARK RED (CAP 22) TAIL (TI) : -CRUSTED AREA(S); ALONG ENTIRE LENGTH: MULTIPLE DARK BROWN AND DRY CRUSTED AREAS, UP TO 3 MM IN DIAMETER (CAP 26) CECUM (CE) : >AUTOLYTIC COLON (CO) : >AUTOLYTIC SKIN (SK) : -M-FIBROSARCOMA,-NEOPLASM PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT EPIDIDYMIDES (EP) : -HYPOSPERMIA,-PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, SKIN/MUSCLE/SUBCUTIS,PRESENT LN, AXILLARY (AX) : -CONGESTION,-PRESENT LN, MANDIBULAR (MN) : -HYPERPLASIA, LYMPHOPLASMACYTIC,-SLIGHT -CONGESTION,-PRESENT TAIL (TI) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT -ACANTHOSIS / HYPERKERATOSIS,-SLIGHT 2243 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 491 ANIMAL NUMBER: C92663 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 02/08/00 STUDY DAY OF DEATH: 660 STUDY WEEK OF DEATH: 95 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 02/08/00 14:13 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), SPLEEN (SP), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), MAMMARY, MALE (MM), COAGULATING GL (CG), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUm /s KULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG), GENERAL COMMENT (GC) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LN, MESENTERIC (MS), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), RECTUM (RE), URINARY BLADDER (UB), SEMINAL VESICLES (SV), HEMATO NEOPLASIA (HN) 2244 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 492 ANIMAL NUMBER: C92664 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 10/11/99 STUDY DAY OF DEATH: 540 STUDY WEEK OF DEATH: 78 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 10/11/99 8:10 PROSECTOR: KEVIN BILLINGS RECORDER: KEVIN BILLINGS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; THIN; VERIFIED AT NECROPSY: NOT OBSERVED >SKIN & PELAGE; ROUGH HAIR COAT; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; RECUMBENT; LATERAL; VERIFIED AT NECROPSY: NOT APPLICABLE >RESPIRATION; LABORED; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); SINGLE MASS, 10 X 8 X 8 MM RED, DARK RED, TAN AND SOFT HISTOPATHOLOGY BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL ADRENAL, CORTEX (AC) : -PIGMENT,-MINIMAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATE PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE 2245 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 493 ANIMAL NUMBER: C92664 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 10/11/99 STUDY DAY OF DEATH: 540 STUDY WEEK OF DEATH: 78 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 10/11/99 8:10 PROSECTOR: KEVIN BILLINGS RECORDER: KEVIN BILLINGS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG), GENERAL COMMENT (GC) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) (CE), 2246 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 494 ANIMAL NUMBER: C92665 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/24/99 STUDY DAY OF DEATH: 614 STUDY WEEK OF DEATH: 88 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/24/99 8:19 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; SWOLLEN; PAW-HIND-RIGHT; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY ADRENAL, CORTEX (AC) : -LARGE; LEFT: 12 X 10 X 10 MM (RIGHT IS 5 X 4 X 4 MM) HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SLIGHT SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SLIGHT PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL -ATROPHY, ACINAR,-MINIMAL ADRENAL, CORTEX (AC) : >UNREMARKABLE ADRENAL, MEDULLA (MA) : -B-PHEOCHROMOCYTOMA,-NEOPLASM PRESENT MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,-MODERATE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATELY SEVERE 2247 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 495 ANIMAL NUMBER: C92665 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/24/99 STUDY DAY OF DEATH: 614 STUDY WEEK OF DEATH: 88 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/24/99 8:19 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY JOINT, OTHER (JT) : -LARGE; RIGHT TARSAL: UP TO 15 MM IN DIAMETER; COLLECTED GENERAL COMMENT (GC) : -ANIMAL OBESE LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL HEART (HT) : -CARDIOMYOPATHY,-SLIGHT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT JEJUNUM (JE) : >AUTOLYTIC ILEUM (IL) : >AUTOLYTIC CECUM (CE) : >AUTOLYTIC URINARY BLADDER (UB) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE CAUSE OF DEATH (CD) : -POSSIBLE TRAUMATIC INJURY,-PRESENT JOINT, OTHER (JT) : -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL -PROBABLE FRACTURE,-PRESENT 2248 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 496 ANIMAL NUMBER: C92665 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/24/99 STUDY DAY OF DEATH: 614 STUDY WEEK OF DEATH: 88 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/24/99 8:19 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAr /i LIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), COLON (CO), RECTUM (RE), SKIN (SK), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2249 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 497 ANIMAL NUMBER: C92666 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/01/99 STUDY DAY OF DEATH: 530 STUDY WEEK OF DEATH: 76 TERMINAL BODY WEIGHT: 529.2 GRAMS DATE AND TIME OF NECROPSY: 10/01/99 13:55 PROSECTOR: LYNN HORNER RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; THIN; VERIFIED AT NECROPSY: NOT OBSERVED >APPEARANCE; HEAD TILT; RIGHT; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; ROUGH HAIR COAT; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 15 X 10 X 7 MM, DARK RED, RED, TAN AND SOFT HISTOPATHOLOGY BRAIN (BR) : >UNREMARKABLE LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MINIMAL PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL ADRENAL, CORTEX (AC) : -PIGMENT,-MINIMAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),SEVERE PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT 2250 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 498 ANIMAL NUMBER: C92666 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/01/99 STUDY DAY OF DEATH: 530 STUDY WEEK OF DEATH: 76 TERMINAL BODY WEIGHT: 529.2 GRAMS DATE AND TIME OF NECROPSY: 10/01/99 13:55 PROSECTOR: LYNN HORNER RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PROSTATE (PR) : -NODULAR; PROXIMAL SURROUNDING URETHRA: GREEN AND FIRM GENERAL COMMENT (GC) : -BLOOD FILM TAKEN CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 2251 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 499 ANIMAL NUMBER: C92666 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/01/99 STUDY DAY OF DEATH: 530 STUDY WEEK OF DEATH: 76 TERMINAL BODY WEIGHT: 529.2 GRAMS DATE AND TIME OF NECROPSY: 10/01/99 13:55 PROSECTOR: LYNN HORNER RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUm /s KULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) (CE), 2252 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 500 ANIMAL NUMBER: C92667 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/20/99 STUDY DAY OF DEATH: 549 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: 548.9 GRAMS DATE AND TIME OF NECROPSY: 10/20/99 13:10 PROSECTOR: JARED FISHEL RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); VENTRAL-ABDOMINAL-RIGHT; WEEK 69; 30 X 30MM; VERIFIED AT NECROPSY: NOT OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; RED HAIR COAT; HEADENTIRE; VERIFIED AT NECROPSY: NOT OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: NOT OBSERVED >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE DARK RED AREAS, UP TO 3 MM IN DIAMETER; COLLECTED ON ROUTINE SECTIONS HISTOPATHOLOGY EYE (EY) : -DYSTROPHY, CORNEAL IN EARLY STAGES, CONSISTS PRIMARILY OF FOCAL MINERALIZATION,-MINIMAL BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL -ANGIECTASIS,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MINIMAL PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,SLIGHT ADRENAL, CORTEX (AC) : -PIGMENT,-MINIMAL 2253 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 501 ANIMAL NUMBER: C92667 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/20/99 STUDY DAY OF DEATH: 549 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: 548.9 GRAMS DATE AND TIME OF NECROPSY: 10/20/99 13:10 PROSECTOR: JARED FISHEL RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY THYROID (TY) : -LARGE; RIGHT: 7 X 6 X 4 MM (LEFT IS 4 X 3 X 2 MM) PITUITARY (PI) : -MASS(ES); 11 X 8 X 6 MM, DARK RED, GRAY AND SOFT URINARY BLADDER (UB) : -SEMIFLUID MATERIAL; FILLED WITH A REDDISH-GREEN FLUID GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: RED AND DRY MATERIAL THYMUS (TH) : >SECTION EXAMINED; TISSUE NOT PRESENT THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT URINARY BLADDER (UB) : -HEMORRHAGE,-MODERATE -PROTEINACEOUS MATERIAL, LUMINAL,PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 2254 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 502 ANIMAL NUMBER: C92667 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/20/99 STUDY DAY OF DEATH: 549 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: 548.9 GRAMS DATE AND TIME OF NECROPSY: 10/20/99 13:10 PROSECTOR: JARED FISHEL RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) (MS), THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) (SE), 2255 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 503 ANIMAL NUMBER: C92668 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 310.4 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 11:25 PROSECTOR: JODI THIEREN RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN STATUS LF ADRENAL (AD0) RT ADRENAL (AD1) BRAIN (BR) LF KIDNEY (KD0) RT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LF TESTIS (TE0) RT TESTIS (TE1) LF THYROID/PARA RT THYROID/PARA (TT0) (TT1) ------------------------------------ 12.3505 ------------------------------------------- ------------------------------------ 3.9789 % ------------------------------------------- ------------------------------------------------------------------------------------- NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN WEIGHT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS 2256 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 504 ANIMAL NUMBER: C92668 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 310.4 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 11:25 PROSECTOR: JODI THIEREN RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) 2257 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 505 ANIMAL NUMBER: C92669 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 812.0 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 13:18 PROSECTOR: TONY HANFELD RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 7 X 6 X 5 MM, RED, DARK RED AND SOFT HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL PANCREAS (PA) : -INFILTRATION, FAT, INTERSTITIAL,SLIGHT TESTIS (TE) : -ATROPHY/DEGENERATION,-MINIMAL -B-INTERSTITIAL CELL TUMOR,-NEOPLASM PRESENT -INFILTRATE, LYMPHOHISTIOCYTIC,-SLIGHT THYROID (TY) : -HYPERPLASIA, C-CELL,-MODERATE -B-ADENOMA, C-CELL,-NEOPLASM PRESENT -B-ADENOMA, FOLLICULAR CELL,-NEOPLASM PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT -HYPERPLASIA,-MINIMAL 2258 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 506 ANIMAL NUMBER: C92669 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 812.0 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 13:18 PROSECTOR: TONY HANFELD RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY STOMACH, GL (ST) : -THICKENED WALL; DIFFUSE MAMMARY, MALE (MM) : -CYST(S); BILATERAL PECTORAL REGION: MULTIPLE WHITE FLUID FILLED CYSTS, UP TO 4 MM IN DIAMETER (CAP 22) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN STOMACH, GL (ST) : -HYPERPLASIA, EPITHELIAL, GLANDULAR,MODERATE CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT MAMMARY, MALE (MM) : -GALACTOCELE,-PRESENT 2259 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 507 ANIMAL NUMBER: C92669 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 812.0 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 13:18 PROSECTOR: TONY HANFELD RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOt /f OOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PARATHYROID (PT), URINARY BLADDER (UB) 2260 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 508 ANIMAL NUMBER: C92670 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/13/99 STUDY DAY OF DEATH: 512 STUDY WEEK OF DEATH: 74 TERMINAL BODY WEIGHT: 374.4 GRAMS DATE AND TIME OF NECROPSY: 09/13/99 10:52 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; PALE; ENTIRE BODY; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; ROUGH HAIR COAT; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; THIN; VERIFIED AT NECROPSY: NOT OBSERVED >APPEARANCE; HUNCHED; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY LIVER (LI) : -LARGE; ALL LOBES -MASS(ES); RIGHT MEDIAN LOBE, ALONG MARGIN: SINGLE MASS, 2 0 X 2 0 X 7 MM, RED, TAN, BROWN, AND SOFT; SAME ON CUT SURFACE; COLLECTED ON ROUTINE SECTION; REGIONAL LN (HEPATIC) NORMAL (CAP 23) -DIFFUSELY LIGHT; ALL LOBES: TAN KIDNEY (KD) : -ROUGH SURFACE, DIFFUSE; BILATERAL -DIFFUSELY LIGHT; BILATERAL: TAN SPLEEN (SP) : -DIFFUSELY LIGHT; RED HISTOPATHOLOGY BRAIN (BR) : -DEGENERATION, FOCAL,-MINIMAL LIVER (LI) : -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL -VACUOLATION, HEPATOCELLULAR,-SLIGHT -B-ADENOMA, HEPATOCELLULAR,-NEOPLASM PRESENT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SEVERE SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SEVERE ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-SLIGHT -HEMATOPOIESIS, EXTRAMEDULLARY,-SLIGHT MARROW, FEMUR (FM) : -HYPERPLASIA, ERYTHROID, MARROW,MODERATELY SEVERE MARROW, STERNUM (SE) : -HYPERPLASIA, ERYTHROID, MARROW,-SLIGHT 2261 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 509 ANIMAL NUMBER: C92670 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/13/99 STUDY DAY OF DEATH: 512 STUDY WEEK OF DEATH: 74 TERMINAL BODY WEIGHT: 374.4 GRAMS DATE AND TIME OF NECROPSY: 09/13/99 10:52 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY THYMUS (TH) : -GELATINOUS; CLEAR STOMACH, GL (ST) : -THICKENED WALL; MUCOSA, CRANIAL ONE HALF, AT JUNCTION OF NONGLANDULAR SEMINAL VESICLES (SV) : -SMALL; BILATERAL: EACH IS 12 X 6 X 2 MM THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATELY SEVERE LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,SLIGHT -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYALINE MATERIAL, ALVEOLAR SEPTA,MODERATE -HYPERPLASIA, TYPE II PNEUMOCYTE,SLIGHT HEART (HT) : -CARDIOMYOPATHY,-SLIGHT PARATHYROID (PT) : -HYPERPLASIA,-MODERATELY SEVERE STOMACH, GL (ST) : -HYPERPLASIA, EPITHELIAL, GLANDULAR,MODERATE -MINERALIZATION, GLANDULAR MUCOSA,MODERATE URINARY BLADDER (UB) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,MODERATELY SEVERE SEMINAL VESICLES (SV) : -SECRETION, DECREASED,-PRESENT 2262 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 510 ANIMAL NUMBER: C92670 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/13/99 STUDY DAY OF DEATH: 512 STUDY WEEK OF DEATH: 74 TERMINAL BODY WEIGHT: 374.4 GRAMS DATE AND TIME OF NECROPSY: 09/13/99 10:52 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY LN, MEDIASTINAL (ML) : -LARGE; UP TO 5 X 3 X 2 MM (CAP 22) -MOTTLED; RED AND TAN (CAP 22) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN EPIDIDYMIDES (EP) : -IMMATURE SPERM FORMS,-MODERATE CAUSE OF DEATH (CD) : -NEPHROPATHY,-PRESENT LN, MEDIASTINAL (ML) : -HEMORRHAGE,-PRESENT LN, OTHER (LN) : >UNREMARKABLE >NOTE:>HEPATIC LN 2263 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 511 ANIMAL NUMBER: C92670 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/13/99 STUDY DAY OF DEATH: 512 STUDY WEEK OF DEATH: 74 TERMINAL BODY WEIGHT: 374.4 GRAMS DATE AND TIME OF NECROPSY: 09/13/99 10:52 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), EPIDIDYMIDES (EP), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), HEMATO NEOPLASIA (HN) 2264 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 512 ANIMAL NUMBER: C92671 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 01/29/00 STUDY DAY OF DEATH: 650 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 01/29/00 7:22 PROSECTOR: TROY ELLENBECKER RECORDER: TROY ELLENBECKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -DEGENERATION, CYSTIC,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MINIMAL PANCREAS (PA) : -INFILTRATION, FAT, INTERSTITIAL,SLIGHT ADRENAL, CORTEX (AC) : -VACUOLATION, DIFFUSE,-MINIMAL ADRENAL, MEDULLA (MA) : -HYPERPLASIA, MEDULLARY,-SLIGHT MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,-SLIGHT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATELY SEVERE HEART (HT) : -CARDIOMYOPATHY,-SLIGHT 2265 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 513 ANIMAL NUMBER: C92671 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 01/29/00 STUDY DAY OF DEATH: 650 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 01/29/00 7:22 PROSECTOR: TROY ELLENBECKER RECORDER: TROY ELLENBECKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -DARK FOCUS(I)/AREA(S); MID ASPECT: SINGLE DARK RED AREA, 5 MM IN DIAMETER LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL: (CAP 22) GENERAL COMMENT (GC) : -MALOCCLUSION; BILATERAL; LOWER INCISORS -DISCHARGE-OCULAR; LEFT: DRY RED MATERIAL -STAINS-PERINEUM/PERIANAL; PERINEUM: MOIST BROWN FLUID PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT EPIDIDYMIDES (EP) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL CAUSE OF DEATH (CD) : -UNDETERMINED MICROSCOPICALLY,-PRESENT LN, MANDIBULAR (MN) : -HYPERPLASIA, LYMPHOPLASMACYTIC,MODERATE -CONGESTION,-PRESENT 2266 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 514 ANIMAL NUMBER: C92671 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 01/29/00 STUDY DAY OF DEATH: 650 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 01/29/00 7:22 PROSECTOR: TROY ELLENBECKER RECORDER: TROY ELLENBECKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), HEMATO NEOPLASIA (HN) 2267 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 515 ANIMAL NUMBER: C92672 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/13/00 STUDY DAY OF DEATH: 665 STUDY WEEK OF DEATH: 95 TERMINAL BODY WEIGHT: 732.1 GRAMS DATE AND TIME OF NECROPSY: 02/13/00 14:54 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >MASS(ES); DORSAL-CERVICAL-LEFT; WEEK 95; VERIFIED AT NECROPSY: OBSERVED >EYES; RED DISCHARGE; EYE-LEFT; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -LIGHT FOCUS(I)/AREA(S); AT JUNCTION OF MEDIAN LOBES: SINGLE TAN AREA, 5 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION HISTOPATHOLOGY BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-SLIGHT -HYPERTROPHY, HEPATOCELLULAR, PERIPORTAL,-SLIGHT -LIPIDOSIS, SUBCAPSULAR (TENSION LIPIDOSIS),-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, PELVIC,-SLIGHT -HYPERPLASIA, TRANSITIONAL CELL,MINIMAL -INFLAMMATION, SUPPURATIVE, PELVIC,SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE PANCREAS (PA) : -B-ADENOMA, ISLET CELL,-NEOPLASM PRESENT -INFILTRATION, FAT, INTERSTITIAL,SLIGHT 2268 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 516 ANIMAL NUMBER: C92672 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/13/00 STUDY DAY OF DEATH: 665 STUDY WEEK OF DEATH: 95 TERMINAL BODY WEIGHT: 732.1 GRAMS DATE AND TIME OF NECROPSY: 02/13/00 14:54 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 10 X 8 X 8 MM, DARK RED, GRAY AND SOFT STOMACH, NONGL (SU) : -THICKENED WALL; DIFFUSE -LIGHT FOCUS(I)/a REA(S); MUCOSA: MULTIPLE TAN AREAS, UP TO 2 X 1 MM ADRENAL, CORTEX (AC) : -PIGMENT,-SLIGHT MARROW, FEMUR (FM) : -HYPOCELLULAR, MARROW,-MODERATELY SEVERE MARROW, STERNUM (SE) : -HYPOCELLULAR, MARROW,-MODERATELY SEVERE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATELY SEVERE LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL HEART (HT) : -CARDIOMYOPATHY,-SLIGHT THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : -HYPERPLASIA, EPITHELIAL, GLANDULAR,MODERATE STOMACH, NONGL (SU) : -ACANTHOSIS / HYPERKERATOSIS,-MODERATE -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE 2269 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 517 ANIMAL NUMBER: C92672 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/13/00 STUDY DAY OF DEATH: 665 STUDY WEEK OF DEATH: 95 TERMINAL BODY WEIGHT: 732.1 GRAMS DATE AND TIME OF NECROPSY: 02/13/00 14:54 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY SKIN (SK) : -MASS(ES); LEFT DORSAL THORACIC REGION: SINGLE MASS, 12 X 8 X 6 MM, DARK RED, TAN AND FIRM; SAME ON CUT SURFACE (CAP 22); OVERLYING SKIN HAS A SINGLE DARK RED AND DRY CRUSTED AREA, 1 MM IN DIAMETER; COLLECTED INTACT WITH MASS; REGIONAL LN (AXILLARY) NORMAL (CAP 23) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DARK RED AND DRY SKIN (SK) : -B-KERATOACANTHOMA,-NEOPLASM PRESENT URINARY BLADDER (UB) : -HYPERPLASIA, TRANSITIONAL CELL,MODERATE -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SEVERE SEMINAL VESICLES (SV) : -SECRETION, DECREASED,-PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 2270 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 518 ANIMAL NUMBER: C92672 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/13/00 STUDY DAY OF DEATH: 665 STUDY WEEK OF DEATH: 95 TERMINAL BODY WEIGHT: 732.1 GRAMS DATE AND TIME OF NECROPSY: 02/13/00 14:54 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) (MS), THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN), LN, AXILLARY (AX) 2271 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 519 ANIMAL NUMBER: C92673 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 02/07/00 STUDY DAY OF DEATH: 659 STUDY WEEK OF DEATH: 95 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 02/07/00 8:56 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); AXILLARY REGION-LEFT; WEEK 81; 50 X 60MM; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 10 X 8 X 8 MM, TAN AND SOFT HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE PANCREAS (PA) : -ATROPHY, ACINAR,-MINIMAL ADRENAL, MEDULLA (MA) : -HYPERPLASIA, MEDULLARY,-SLIGHT -B-PHEOCHROMOCYTOMA,-NEOPLASM PRESENT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATELY SEVERE HEART (HT) : -CARDIOMYOPATHY,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT JEJUNUM (JE) : >AUTOLYTIC 2272 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 520 ANIMAL NUMBER: C92673 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 02/07/00 STUDY DAY OF DEATH: 659 STUDY WEEK OF DEATH: 95 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 02/07/00 8:56 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY MAMMARY, MALE (MM) : -MASS(ES); LEFT AXILLARY REGION: SINGLE MASS, 65 X 50 X 30 MM, RED, TAN, GRAY, LOBULATED AND FIRM; SAME ON CUT SURFACE (CAP 23); REGIONAL LN (AXILLARY) NORMAL (CAP 22) ILEUM (IL) : >AUTOLYTIC CECUM (CE) : >AUTOLYTIC SKIN (SK) : -B-FIBROMA,-NEOPLASM PRESENT PROSTATE (PR) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT MAMMARY, MALE (MM) : >NOT REQUIRED TO BE EXAMINED FOR ANIMAL >NOTE:>SEE SKIN FOR DIAGNOSIS OF MASS 2273 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 521 ANIMAL NUMBER: C92673 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 02/07/00 STUDY DAY OF DEATH: 659 STUDY WEEK OF DEATH: 95 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 02/07/00 8:56 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUm /s KULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG), GENERAL COMMENT (GC) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, CORTEX (AC), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN), LN, AXILLARY (AX) (SN), 2274 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 522 ANIMAL NUMBER: C92674 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 930.5 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 8:21 PROSECTOR: TONY HANFELD RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) TESTIS (TE) : -CONTAINS FLUID; LEFT: CLEAR FLUID -LARGE; LEFT: 4 5 X 2 5 X 2 0 MM (RIGHT IS 20 X 15 X 10 MM) PITUITARY (PI) : -MASS(ES); 5 X 5 X 4 MM, RED, TAN, AND FIRM STOMACH, GL (ST) : -THICKENED WALL; DIFFUSE HISTOPATHOLOGY BRAIN (BR) : >UNREMARKABLE LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -NECROSIS, INDIVIDUAL HEPATOCYTE,MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-SLIGHT -DEGENERATION, CYSTIC,-MINIMAL PANCREAS (PA) : -INFILTRATE, LYMPHOHISTIOCYTIC,-SLIGHT -ATROPHY, ACINAR,-MODERATE TESTIS (TE) : -ATROPHY/DEGENERATION,-SEVERE >NOTE:>UNILATERAL THYROID (TY) : -HYPERPLASIA, C-CELL,-SLIGHT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : -INFLAMMATION, ACUTE,-MINIMAL -HYPERPLASIA, EPITHELIAL, GLANDULAR,SLIGHT 2275 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 523 ANIMAL NUMBER: C92674 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 930.5 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 8:21 PROSECTOR: TONY HANFELD RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -ANIMAL OBESE CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUm /s KULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: KIDNEY (KD), LUNG (LU), PARATHYROID (PT), URINARY BLADDER (UB) 2276 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 524 ANIMAL NUMBER: C92675 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/31/00 STUDY DAY OF DEATH: 712 STUDY WEEK OF DEATH: 102 TERMINAL BODY WEIGHT: 503.9 GRAMS DATE AND TIME OF NECROPSY: 03/31/00 10:45 PROSECTOR: JERALD W. JOHNSON RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); DORSAL; WEEK 90; 15 X 15MM; VERIFIED AT NECROPSY: OBSERVED >EYES; RED DISCHARGE; EYE-LEFT; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: NOT OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) HISTOPATHOLOGY BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MODERATE -DEGENERATION, CYSTIC,-MINIMAL -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, PELVIC,-SLIGHT -HYPERPLASIA, TRANSITIONAL CELL,MODERATE -INFLAMMATION, SUPPURATIVE, PELVIC,SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE 2277 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 525 ANIMAL NUMBER: C92675 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/31/00 STUDY DAY OF DEATH: 712 STUDY WEEK OF DEATH: 102 TERMINAL BODY WEIGHT: 503.9 GRAMS DATE AND TIME OF NECROPSY: 03/31/00 10:45 PROSECTOR: JERALD W. JOHNSON RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 12 X 10 X 6 MM, DARK RED, BROWN AND SOFT SKIN (SK) : -MASS(ES); RIGHT DORSAL LUMBAR REGION: SINGLE MASS, 20 X 15 X 7 MM, TAN AND FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED PROSTATE (PR) : -LARGE; 30 X 20 X 18 MM PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL ADRENAL, CORTEX (AC) : -PIGMENT,-MINIMAL MARROW, FEMUR (FM) : -HYPOCELLULAR, MARROW,-MODERATE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATE HEART (HT) : -CARDIOMYOPATHY,-MINIMAL THYROID (TY) : -DILATATION / CYST, FOLLICULAR,-PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT SKIN (SK) : -CYST, EPIDERMAL INCLUSION,-PRESENT URINARY BLADDER (UB) : -HYPERPLASIA, TRANSITIONAL CELL,MODERATE -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SEVERE 2278 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 526 ANIMAL NUMBER: C92675 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/31/00 STUDY DAY OF DEATH: 712 STUDY WEEK OF DEATH: 102 TERMINAL BODY WEIGHT: 503.9 GRAMS DATE AND TIME OF NECROPSY: 03/31/00 10:45 PROSECTOR: JERALD W. JOHNSON RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PROSTATE (PR) : -GELATINOUS; DIFFUSE: LIGHT GREEN SEMINAL VESICLES (SV) : -SMALL; BILATERAL: LEFT IS 10 X 7 X 3 MM, RIGHT IS 15 X 8 X 5 MM BILE DUCT (BD) : -LARGE; UP TO 5 MM IN DIAMETER (CAP 23) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; LEFT: DARK RED DRY MATERIAL SEMINAL VESICLES (SV) : -SECRETION, DECREASED,-PRESENT -INFLAMMATION, CHRONIC-ACTIVE,MODERATELY SEVERE EPIDIDYMIDES (EP) : -VACUOLATION, TUBULAR EPITHELIUM,SLIGHT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT BILE DUCT (BD) : -CYST,-PRESENT -INFLAMMATION, CHRONIC,-MODERATELY SEVERE -THROMBUS,-PRESENT 2279 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 527 ANIMAL NUMBER: C92675 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/31/00 STUDY DAY OF DEATH: 712 STUDY WEEK OF DEATH: 102 TERMINAL BODY WEIGHT: 503.9 GRAMS DATE AND TIME OF NECROPSY: 03/31/00 10:45 PROSECTOR: JERALD W. JOHNSON RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, STERNUM BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), HEMATO NEOPLASIA (HN) (SE), (TR), 2280 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 528 ANIMAL NUMBER: C92676 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 448.1 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 12:55 PROSECTOR: DONIA MEYER RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT' (%) LF ADRENAL (AD0) RT ADRENAL (AD1) BRAIN (BR) LF KIDNEY (KD0) RT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LF TESTIS (TE0) RT TESTIS (TE1) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) .0301 .0281 2.2089 1.8605 1.8648 13.3586 1.4477 .6389 1.7039 1.6819 .0121 .0108 .0067 % .0063 % .4929 % .4152 % .4162 % 2.9812 % .3231 % .1426 % .3802 % .3753 % .0027 % .0024 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0136 .0127 1.0000 .8423 .8442 6.0476 .6554 .2892 .7714 .7614 .0055 .0049 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL KIDNEY (KD) : -REGENERATION, TUBULAR EPITHELIUM,MINIMAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2281 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 529 ANIMAL NUMBER: C92676 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 448.1 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 12:55 PROSECTOR: DONIA MEYER RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE) 2282 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 530 ANIMAL NUMBER: C92677 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/09/00 STUDY DAY OF DEATH: 690 STUDY WEEK OF DEATH: 99 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/09/00 8:13 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >WART LIKE LESION(S); DORSAL; WEEK 80; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -LARGE; ALL LOBES: MARGINS APPEAR ROUNDED -LIGHT FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE TAN AREAS, UP TO 1 MM IN DIAMETER; COLLECTED ON ROUTINE SECTIONS HISTOPATHOLOGY BRAIN (BR) : -MINERALIZATION,-MINIMAL LIVER (LI) : -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MODERATELY SEVERE -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, PERIPORTAL,-MODERATE KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE PANCREAS (PA) : -B-ADENOMA, ISLET CELL,-NEOPLASM PRESENT -INFILTRATION, FAT, INTERSTITIAL,SLIGHT ADRENAL, CORTEX (AC) : -HYPERPLASIA, CORTICAL,-MINIMAL -PIGMENT,-MINIMAL 2283 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 531 ANIMAL NUMBER: C92677 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/09/00 STUDY DAY OF DEATH: 690 STUDY WEEK OF DEATH: 99 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/09/00 8:13 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY SKIN (SK) : -RAISED FOCUS(I)/AREA(S); DORSAL LUMBAR REGION: SINGLE DRY TAN, CRUSTED RAISED AREA, 10 X 10 X 7 MM (CAP 22) MARROW, STERNUM (SE) : -HYPERPLASIA, ERYTHROID, MARROW,MODERATE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATE HEART (HT) : -CARDIOMYOPATHY,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT -HYPERPLASIA,-MINIMAL HARDERIAN GLAND (HG) : -ATROPHY,-MINIMAL JEJUNUM (JE) : >AUTOLYTIC CECUM (CE) : >AUTOLYTIC COLON (CO) : >AUTOLYTIC RECTUM (RE) : >AUTOLYTIC SKIN (SK) : -B-KERATOACANTHOMA,-NEOPLASM PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT 2284 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 532 ANIMAL NUMBER: C92677 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/09/00 STUDY DAY OF DEATH: 690 STUDY WEEK OF DEATH: 99 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/09/00 8:13 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY CAUSE OF DEATH (CD) : -UNDETERMINED MICROSCOPICALLY,-PRESENT THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG), GENERAL COMMENT (GC) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), ILEUM (IL), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2285 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 533 ANIMAL NUMBER: C92678 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 274.6 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 11:54 PROSECTOR: LYNN HORNER RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN STATUS LF ADRENAL (AD0) RT ADRENAL (AD1) BRAIN (BR) LF KIDNEY (KD0) RT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LF TESTIS (TE0) RT TESTIS (TE1) LF THYROID/PARA RT THYROID/PARA (TT0) (TT1) ------------------------------------ 10.0762 ------------------------------------------- ------------------------------------ 3.6700 % ------------------------------------------- ------------------------------------------------------------------------------------- NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN WEIGHT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS 2286 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 534 ANIMAL NUMBER: C92678 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 274.6 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 11:54 PROSECTOR: LYNN HORNER RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) 2287 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 535 ANIMAL NUMBER: C92679 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/07/99 STUDY DAY OF DEATH: 383 STUDY WEEK OF DEATH: 55 TERMINAL BODY WEIGHT: 698.8 GRAMS DATE AND TIME OF NECROPSY: 05/07/99 14:08 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >EYES; RED DISCHARGE; EYES; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >RESPIRATION; IRREGULAR; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -HEPATIC ANOMALY (LOBULE); DIAPHRAGMATIC SURFACE AT JUNCTION OF MEDIAN LOBES: SINGLE ANOMALY, 8 X 5 X 3 MM, LIVER-LIKE IN CONSISTENCY (CAP 21) PITUITARY (PI) : -MASS(ES); 8 MM IN DIAMETER, RED, DARK RED, GRAY, AND SOFT HISTOPATHOLOGY BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-SLIGHT -ANOMALY, SECONDARY TO DIAPHRAGMATIC HERNIA,-PRESENT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),SLIGHT HEART (HT) : -CARDIOMYOPATHY,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT 2288 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 536 ANIMAL NUMBER: C92679 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/07/99 STUDY DAY OF DEATH: 383 STUDY WEEK OF DEATH: 55 TERMINAL BODY WEIGHT: 698.8 GRAMS DATE AND TIME OF NECROPSY: 05/07/99 14:08 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PROSTATE (PR) : -NODULAR; PROXIMAL, SURROUNDING URETHRA: GREEN AND FIRM SEMINAL VESICLES (SV) : -GELATINOUS; BILATERAL: CLEAR GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DARK RED, DRY, AND CRUSTED PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,MODERATELY SEVERE SEMINAL VESICLES (SV) : -SECRETION, INCREASED,-PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 2289 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 537 ANIMAL NUMBER: C92679 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/07/99 STUDY DAY OF DEATH: 383 STUDY WEEK OF DEATH: 55 TERMINAL BODY WEIGHT: 698.8 GRAMS DATE AND TIME OF NECROPSY: 05/07/99 14:08 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2290 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 538 ANIMAL NUMBER: C92680 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/23/99 STUDY DAY OF DEATH: 583 STUDY WEEK OF DEATH: 84 TERMINAL BODY WEIGHT: 448.1 GRAMS DATE AND TIME OF NECROPSY: 11/23/99 15:03 PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; PALE; ENTIRE BODY; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; THIN; VERIFIED AT NECROPSY: NOT OBSERVED >EYES; SQUINTED; PERI-ORBITAL; VERIFIED AT NECROPSY: NOT APPLICABLE >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; HUNCHED; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, PELVIC,-MODERATE SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL ADRENAL, CORTEX (AC) : -ATROPHY, CORTICAL,-MODERATE TESTIS (TE) : -INFLAMMATION, VASCULAR,-MINIMAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATELY SEVERE 2291 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 539 ANIMAL NUMBER: C92680 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/23/99 STUDY DAY OF DEATH: 583 STUDY WEEK OF DEATH: 84 TERMINAL BODY WEIGHT: 448.1 GRAMS DATE AND TIME OF NECROPSY: 11/23/99 15:03 PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 12 X 11 X 7 MM, DARK RED RED AND SOFT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE SEMINAL VESICLES (SV) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE -ATROPHY,-MODERATELY SEVERE CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 2292 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 540 ANIMAL NUMBER: C92680 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/23/99 STUDY DAY OF DEATH: 583 STUDY WEEK OF DEATH: 84 TERMINAL BODY WEIGHT: 448.1 GRAMS DATE AND TIME OF NECROPSY: 11/23/99 15:03 PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2293 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 541 ANIMAL NUMBER: C92681 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/16/99 STUDY DAY OF DEATH: 606 STUDY WEEK OF DEATH: 87 TERMINAL BODY WEIGHT: 444.9 GRAMS DATE AND TIME OF NECROPSY: 12/16/99 15:05 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); TAIL-PROXIMAL; WEEK 62; 20 X 10MM; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) HISTOPATHOLOGY BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-SLIGHT -FIBROSIS, PERIBILIARY,-SLIGHT -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MODERATE SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL -INFILTRATION, FAT, INTERSTITIAL,SLIGHT MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,MODERATELY SEVERE 2294 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 542 ANIMAL NUMBER: C92681 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/16/99 STUDY DAY OF DEATH: 606 STUDY WEEK OF DEATH: 87 TERMINAL BODY WEIGHT: 444.9 GRAMS DATE AND TIME OF NECROPSY: 12/16/99 15:05 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 11 X 9 X 8 MM, RED, GREY, TAN, LOBULATED AND SOFT STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: MULTIPLE BROWN FOCI, UP TO 1 MM IN DIAMETER PROSTATE (PR) : -NODULAR; PROXIMAL, SURROUNDING URETHRA: GREEN AND FIRM -LARGE; 31 X 15 X 8 MM MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,MODERATELY SEVERE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATELY SEVERE LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL HEART (HT) : -CARDIOMYOPATHY,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL STOMACH, GL (ST) : >UNREMARKABLE URINARY BLADDER (UB) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT SEMINAL VESICLES (SV) : -SECRETION, INCREASED,-PRESENT 2295 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 543 ANIMAL NUMBER: C92681 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/16/99 STUDY DAY OF DEATH: 606 STUDY WEEK OF DEATH: 87 TERMINAL BODY WEIGHT: 444.9 GRAMS DATE AND TIME OF NECROPSY: 12/16/99 15:05 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY SEMINAL VESICLES (SV) : -LARGE; BILATERAL: EACH IS 32 X 21 X 6 MM TAIL (TI) : -RAISED AREA(S); ALONG ENTIRE LENGTH OF TAIL: MULTIPLE TAN AND DARK BROWN RAISED AREAS, UP TO 16 X 9 X 6 MM; POSSIBLY CONSISTENT WITH MASS (TAIL) AS NOTED IN CLINICAL OBSERVATIONS; PORTION COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN EPIDIDYMIDES (EP) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT TAIL (TI) : -INFLAMMATION, CHRONIC-ACTIVE,MODERATELY SEVERE -ACANTHOSIS / HYPERKERATOSIS,-SEVERE 2296 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 544 ANIMAL NUMBER: C92681 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/16/99 STUDY DAY OF DEATH: 606 STUDY WEEK OF DEATH: 87 TERMINAL BODY WEIGHT: 444.9 GRAMS DATE AND TIME OF NECROPSY: 12/16/99 15:05 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), HEMATO NEOPLASIA (HN) 2297 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 545 ANIMAL NUMBER: C92682 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 887.6 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 13:28 PROSECTOR: TONY HANFELD RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; SORE/SCAB; PAW-HINDRIGHT; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) THYROID (TY) : -LARGE; RIGHT: 6 X 3 X 3 MM (LEFT IS 4 X 2 X 2 MM) PITUITARY (PI) : -MASS(ES); 8 X 8 X 4 MM, RED, DARK RED, TAN, LOBULATED, AND FIRM HISTOPATHOLOGY BRAIN (BR) : >UNREMARKABLE LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -NECROSIS, INDIVIDUAL HEPATOCYTE,MINIMAL -VACUOLATION, HEPATOCELLULAR, MIDZONAL / CENTRILOBULAR,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -DEGENERATION, CYSTIC,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL PANCREAS (PA) : -INFILTRATION, FAT, INTERSTITIAL,SLIGHT LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL THYROID (TY) : -M-CARCINOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT 2298 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 546 ANIMAL NUMBER: C92682 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 887.6 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 13:28 PROSECTOR: TONY HANFELD RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY HARDERIAN GLAND (HG) : -MOTTLED; BILATERAL: BLACK AND TAN STOMACH, GL (ST) : -THICKENED WALL; DIFFUSE PROSTATE (PR) : -NODULAR; SURROUNDING URETHRA: GREEN, BROWN, AND FIRM FOOT/FOOTPAD (FP) : -ABRASION(S)/ULCERATION(S); RIGHT HINDFOOT: SINGLE DARK RED, TAN, AND DRY ABRADED AREA, 5 X 3 MM; COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN HARDERIAN GLAND (HG) : >UNREMARKABLE STOMACH, GL (ST) : -HYPERPLASIA, EPITHELIAL, GLANDULAR,SLIGHT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT FOOT/FOOTPAD (FP) : -INFLAMMATION, CHRONIC-ACTIVE,-SEVERE -ULCERATION,-SLIGHT -ACANTHOSIS / HYPERKERATOSIS,-SLIGHT 2299 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 547 ANIMAL NUMBER: C92682 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 887.6 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 13:28 PROSECTOR: TONY HANFELD RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: TESTIS (TE), PARATHYROID (PT), URINARY BLADDER (UB) 2300 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 548 ANIMAL NUMBER: C92683 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 922.3 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 10:13 PROSECTOR: TERRI L. WILLIAMS RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; SWOLLEN; LIMB-HIND-LEFT; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE DARK RED AREAS, UP TO 6 MM IN DIAMETER; COLLECTED ON ROUTINE SECTIONS STOMACH, GL (ST) : -THICKENED WALL; DIFFUSE PROSTATE (PR) : -NODULAR; PROXIMAL SURROUNDING URETHRA: GREEN AND FIRM HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SLIGHT PANCREAS (PA) : -B-ADENOMA, ISLET CELL,-NEOPLASM PRESENT -INFILTRATION, FAT, INTERSTITIAL,MODERATE PARATHYROID (PT) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : -HYPERPLASIA, EPITHELIAL, GLANDULAR,SLIGHT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2301 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 549 ANIMAL NUMBER: C92683 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 922.3 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 10:13 PROSECTOR: TERRI L. WILLIAMS RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -ANIMAL OBESE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUm /s KULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: TESTIS (TE), LUNG (LU), THYROID (TY), URINARY BLADDER (UB) 2302 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 550 ANIMAL NUMBER: C92684 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 427.6 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 9:38 PROSECTOR: LAURIE J. SCHULLER RECORDER: KIMBERLY DURLAND POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT' (%) LF ADRENAL (AD0) RT ADRENAL (AD1) BRAIN (BR) LF KIDNEY (KD0) RT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LF TESTIS (TE0) RT TESTIS (TE1) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) .0224 .0175 2.2858 1.8556 1.8072 13.5605 2.1432 .6898 1.5608 1.5278 .0078 .0109 .0052 % .0041 % .5346 % .4340 % .4226 % 3.1713 % .5012 % .1613 % .3650 % .3573 % .0018 % .0025 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0098 .0077 1.0000 .8118 .7906 5.9325 .9376 .3018 .6828 .6684 .0034 .0048 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-SLIGHT KIDNEY (KD) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2303 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 551 ANIMAL NUMBER: C92684 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 427.6 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 9:38 PROSECTOR: LAURIE J. SCHULLER RECORDER: KIMBERLY DURLAND POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE) 2304 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 552 ANIMAL NUMBER: C92685 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/25/99 STUDY DAY OF DEATH: 554 STUDY WEEK OF DEATH: 80 TERMINAL BODY WEIGHT: 575.8 GRAMS DATE AND TIME OF NECROPSY: 10/25/99 8:14 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; RECUMBENT; LATERAL; VERIFIED AT NECROPSY: NOT APPLICABLE >EYES; RED DISCHARGE; EYES; VERIFIED AT NECROPSY: OBSERVED >EYES; SQUINTED; PERI-ORBITAL-RIGHT; VERIFIED AT NECROPSY: NOT OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -LIGHT FOCUS(I)/AREA(S); AT JUNCTION OF MEDIAN LOBES: SINGLE TAN AREA, 8 X 5 MM; COLLECTED ON ROUTINE SECTION HISTOPATHOLOGY BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-SLIGHT -DEGENERATION, CYSTIC,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -INFLAMMATION, CHRONIC-ACTIVE, PELVIC,MINIMAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-MINIMAL -ANGIECTASIS,-MINIMAL -PIGMENT,-MINIMAL MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,-SEVERE 2305 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 553 ANIMAL NUMBER: C92685 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/25/99 STUDY DAY OF DEATH: 554 STUDY WEEK OF DEATH: 80 TERMINAL BODY WEIGHT: 575.8 GRAMS DATE AND TIME OF NECROPSY: 10/25/99 8:14 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 8 X 6 X 6 MM, RED, DARK RED, GREY, TAN AND SOFT PROSTATE (PR) : -MASS(ES); INVOLVING ENTIRE ORGAN: SINGLE MASS, 33 X 33 X 23 MM, RED, DARK RED, GREY, TAN, LOBULATED, GELATINOUS AND SOFT; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (SEE LUMBAR) SEMINAL VESICLES (SV) : -LARGE; BILATERAL : EACH IS 28 X 15 X 5 MM LN, RENAL (RL) : -LARGE; LEFT: UP TO 9 X 7 X 4 MM (RIGHT IS UP TO 3 X 2 X 2 MM) (CAP 23) MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,-SEVERE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATELY SEVERE HEART (HT) : -CARDIOMYOPATHY,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT URINARY BLADDER (UB) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SEVERE SEMINAL VESICLES (SV) : -SECRETION, INCREASED,-PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT LN, RENAL (RL) : -HYPERPLASIA, LYMPHOPLASMACYTIC,MODERATELY SEVERE 2306 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 554 ANIMAL NUMBER: C92685 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/25/99 STUDY DAY OF DEATH: 554 STUDY WEEK OF DEATH: 80 TERMINAL BODY WEIGHT: 575.8 GRAMS DATE AND TIME OF NECROPSY: 10/25/99 8:14 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY MAMMARY, MALE (MM) : -CYST(S); BILATERAL LATERAL CERVICAL REGIONS: MULTIPLE TAN, CREAMY SEMIFLUID FILLED CYSTS, UP TO 3 MM IN DIAMETER (CAP 22) LN, LUMBAR/ILIAC (LM) : -LARGE; BILATERAL: RIGHT IS UP TO 7 X 5 X 4 MM, LEFT IS UP TO 6 X 5 X 4 MM (CAP 24) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DRY, RED MATERIAL -ANIMAL THIN MAMMARY, MALE (MM) : -GALACTOCELE,-PRESENT LN, LUMBAR/ILIAC (LM) : -LYMPHANGIECTASIS,-PRESENT -HYPERPLASIA, LYMPHOPLASMACYTIC,MODERATE 2307 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 555 ANIMAL NUMBER: C92685 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/25/99 STUDY DAY OF DEATH: 554 STUDY WEEK OF DEATH: 80 TERMINAL BODY WEIGHT: 575.8 GRAMS DATE AND TIME OF NECROPSY: 10/25/99 8:14 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, MANDIBULAR (MN), COAGULATING GL (CG), TAIL (TI), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) (SU), 2308 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 556 ANIMAL NUMBER: C92686 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/22/99 STUDY DAY OF DEATH: 582 STUDY WEEK OF DEATH: 84 TERMINAL BODY WEIGHT: 622.1 GRAMS DATE AND TIME OF NECROPSY: 11/22/99 8:00 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; RED HAIR COAT; VENTRAL ABDOMINAL; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) HISTOPATHOLOGY BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-MODERATELY SEVERE -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC.-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, PELVIC,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL ADRENAL, MEDULLA (MA) : -HYPERPLASIA, MEDULLARY,-SLIGHT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),SEVERE 2309 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 557 ANIMAL NUMBER: C92686 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/22/99 STUDY DAY OF DEATH: 582 STUDY WEEK OF DEATH: 84 TERMINAL BODY WEIGHT: 622.1 GRAMS DATE AND TIME OF NECROPSY: 11/22/99 8:00 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); SINGLE MASS, 12 X 8 X 7 MM, RED, DARK RED, TAN, GRAY, AND SOFT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL: UPPER INCISORS -STAINS - SKIN/HAIRCOAT; VENTRAL ABDOMINAL REGION, EXTENDING TO PERIANAL REGION: BROWN AND DRY HEART (HT) : -CARDIOMYOPATHY,-SLIGHT THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 2310 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 558 ANIMAL NUMBER: C92686 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/22/99 STUDY DAY OF DEATH: 582 STUDY WEEK OF DEATH: 84 TERMINAL BODY WEIGHT: 622.1 GRAMS DATE AND TIME OF NECROPSY: 11/22/99 8:00 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, CORTEX (AC), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2311 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 559 ANIMAL NUMBER: C92687 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/03/99 STUDY DAY OF DEATH: 502 STUDY WEEK OF DEATH: 72 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 09/03/99 14:44 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >WART LIKE LESION(S); HEAD-MAXILLARYLEFT; WEEK 57; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; ROUGH HAIR COAT; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >EYES; DILATED PUPIL; EYES; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) HISTOPATHOLOGY BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -DEGENERATION, CYSTIC,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATELY SEVERE LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL HEART (HT) : -CARDIOMYOPATHY,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT 2312 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 560 ANIMAL NUMBER: C92687 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/03/99 STUDY DAY OF DEATH: 502 STUDY WEEK OF DEATH: 72 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 09/03/99 14:44 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 7 X 7 X 5 MM, RED, DARK RED, TAN, LOBULATED AND FIRM STOMACH, GL (ST) : -LIGHT FOCUS(I)/AREA(S); MUCOSA: MULTIPLE TAN AREAS, UP TO 3 X 2 MM STOMACH, NONGL (SU) : -LIGHT FOCUS(I)/AREA(S); MUCOSA: MULTIPLE TAN AREAS, UP TO 3 X 2 MM SKIN (SK) : -CRUSTED AREA(S); ALONG LOWER LEFT MANDIBLE: SINGLE TAN CRUSTED AREA, 3 X 2 MM (CAP 21) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN STOMACH, GL (ST) : -INFLAMMATION, ACUTE,-MINIMAL -EROSION,-MINIMAL STOMACH, NONGL (SU) : -ACANTHOSIS / HYPERKERATOSIS,MODERATELY SEVERE -ULCERATION,-SLIGHT -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT SKIN (SK) : -ACANTHOSIS/HYPERKERATOSIS,-MINIMAL PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 2313 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 561 ANIMAL NUMBER: C92687 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/03/99 STUDY DAY OF DEATH: 502 STUDY WEEK OF DEATH: 72 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 09/03/99 14:44 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2314 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 562 ANIMAL NUMBER: C92688 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/25/00 STUDY DAY OF DEATH: 677 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 336.4 GRAMS DATE AND TIME OF NECROPSY: 02/25/00 8:15 PROSECTOR: NANCY DIEDRICH RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; SWOLLEN; PAW-HIND-LEFT; VERIFIED AT NECROPSY: NOT OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -MOTTLED; ALL LOBES: DARK RED AND BROWN KIDNEY (KD) : -CYST(S); BILATERAL, CORTICAL SURFACE: MULTIPLE CLEAR FLUID FILLED CYSTS, PINPOINT AND UP TO 1 MM IN DIAMETER -ROUGH SURFACE, DIFFUSE; BILATERAL -MOTTLED; BILATERAL: RED, BROWN, AND TAN PANCREAS (PA) : -LARGE VESSELS; ALONG DUODENUM: SINGLE VESSEL, 3 MM IN DIAMETER (CAP 24) HISTOPATHOLOGY EYE (EY) : -ATROPHY, RETINAL,-MODERATE BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -NECROSIS, INDIVIDUAL HEPATOCYTE,MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, PERIPORTAL,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SEVERE SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-MODERATE -HYPERPLASIA, ISLET CELLS,-MODERATELY SEVERE 2315 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 563 ANIMAL NUMBER: C92688 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/25/00 STUDY DAY OF DEATH: 677 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 336.4 GRAMS DATE AND TIME OF NECROPSY: 02/25/00 8:15 PROSECTOR: NANCY DIEDRICH RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY ADRENAL, CORTEX (AC) : -LARGE; LEFT: 7 X 5 X 4 MM (RIGHT IS 5 X 4 X 3 MM) PARATHYROID (PT) : -LARGE; BILATERAL: LEFT IS 2 MM IN DIAMETER, RIGHT IS 4 MM IN DIAMETER PITUITARY (PI) : -MASS(ES); 12 X 10 X 8 MM, RED, DARK RED, LOBULATED, AND SOFT PANCREAS (PA) : -ATROPHY, ACINAR,-MINIMAL -INFLAMMATION, VASCULAR,-SLIGHT >NOTE:>SEE ALSO SLIDE #17 ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-SLIGHT -HYPERTROPHY, CORTICAL CELL,-MINIMAL -PIGMENT,-MINIMAL -DEGENERATION / NECROSIS, UNILATERAL,SEVERE ADRENAL, MEDULLA (MA) : -UNILATERALLY EXAMINED,-PRESENT -HYPERPLASIA, MEDULLARY,-SLIGHT TESTIS (TE) : -INFLAMMATION, VASCULAR,-MODERATE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATELY SEVERE HEART (HT) : -CARDIOMYOPATHY,-SLIGHT THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PARATHYROID (PT) : -HYPERPLASIA,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT 2316 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 564 ANIMAL NUMBER: C92688 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/25/00 STUDY DAY OF DEATH: 677 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 336.4 GRAMS DATE AND TIME OF NECROPSY: 02/25/00 8:15 PROSECTOR: NANCY DIEDRICH RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: MULTIPLE DARK BROWN AREAS UP TO 2 X 1 MM PROSTATE (PR) : -NODULAR; PROXIMAL, SURROUNDING URETHRA: GREEN AND FIRM -LARGE; 3 0 X 2 0 X 8 MM SEMINAL VESICLES (SV) : -LARGE; BILATERAL: LEFT IS 35 X 15 X 5 MM, RIGHT IS 30 X 15 X 3 MM LN, RENAL (RL) : -DIFFUSELY RED; BILATERAL: DARK RED (CAP 25) MAMMARY, MALE (MM) : -CYST(S); BILATERAL, AXILLARY REGIONS: MULTIPLE TAN CREAMY SEMIFLUID FILLED CYSTS, 10 X 5 X 5 MM (CAP 22) LN, ANT MES/PANC (AP) : -LARGE; UP TO 5 X 3 X 2 MM (CAP 23) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN STOMACH, GL (ST) : -EROSION,-MINIMAL PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL SEMINAL VESICLES (SV) : -SECRETION, INCREASED,-PRESENT CAUSE OF DEATH (CD) : -NEPHROPATHY,-PRESENT LN, RENAL (RL) : -HYPERPLASIA, LYMPHOPLASMACYTIC,MINIMAL -HEMORRHAGE,-PRESENT -INFILTRATE, MACROPHAGE, PIGMENTED,SLIGHT MAMMARY, MALE (MM) : -GALACTOCELE,-PRESENT -B-FIBROADENOMA,-NEOPLASM PRESENT LN, ANT MES/PANC (AP) : -HYPERPLASIA, LYMPHOPLASMACYTIC,-SLIGHT -INFILTRATE, MACROPHAGE, PIGMENTED,SLIGHT 2317 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 565 ANIMAL NUMBER: C92688 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/25/00 STUDY DAY OF DEATH: 677 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 336.4 GRAMS DATE AND TIME OF NECROPSY: 02/25/00 8:15 PROSECTOR: NANCY DIEDRICH RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -ANIMAL THIN THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), SPLEEN (SP), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, MANDIBULAR (MN), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2318 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 566 ANIMAL NUMBER: C92689 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 05/14/99 STUDY DAY OF DEATH: 390 STUDY WEEK OF DEATH: 56 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 05/14/99 8:26 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 8 X 5 X 5 MM, DARK RED AND SOFT HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL HEART (HT) : -CARDIOMYOPATHY,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT DUODENUM (DU) : >AUTOLYTIC JEJUNUM (JE) : >AUTOLYTIC ILEUM (IL) : >AUTOLYTIC CECUM (CE) : >AUTOLYTIC COLON (CO) : >AUTOLYTIC 2319 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 567 ANIMAL NUMBER: C92689 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 05/14/99 STUDY DAY OF DEATH: 390 STUDY WEEK OF DEATH: 56 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 05/14/99 8:26 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -MALOCCLUSION; BILATERAL UPPER AND LOWER INCISORS -TEETH MISSING/BROKEN/WORN; LEFT LOWER INCISOR: BROKEN; APPROXIMATELY ONEHALF OF INCISOR REMAINING URINARY BLADDER (UB) : >AUTOLYTIC PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 2320 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 568 ANIMAL NUMBER: C92689 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 05/14/99 STUDY DAY OF DEATH: 390 STUDY WEEK OF DEATH: 56 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 05/14/99 8:26 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), RECTUM (RE), SKIN (SK), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2321 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 569 ANIMAL NUMBER: C92690 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 1034.6 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 10:28 PROSECTOR: DAVID SCHUETTE RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; SORE/SCAB; PAW-HINDLEFT; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-MINIMAL -VACUOLATION, HEPATOCELLULAR, MIDZONAL / CENTRILOBULAR,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-SEVERE -DEGENERATION, CYSTIC,-SLIGHT -HYPERPLASIA, BILE DUCT,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SLIGHT PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL -HYPERPLASIA, ISLET CELLS,-MODERATE TESTIS (TE) : -HYPERPLASIA, INTERSTITIAL CELL (LEYDIG CELL),-SLIGHT THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT SALIV GL, MANDIB (SG) : >NOT REQUIRED TO BE EXAMINED FOR ANIMAL 2322 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 570 ANIMAL NUMBER: C92690 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 1034.6 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 10:28 PROSECTOR: DAVID SCHUETTE RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY SALIV GL, MANDIB (SG) : -MASS(ES); LEFT, ATTACHED TO CRANIAL END: SINGLE MASS, 12 X 10 X 8 MM, TAN AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (SEE MANDIBULAR) -DIFFUSELY LIGHT; LEFT: TAN LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL: DARK RED (CAP 23) LN, LUMBAR/ILIAC (LM) : -LARGE; LEFT: 17 X 12 X 6 MM (RIGHT IS 6 X 3 X 2 MM) (CAP 24) FOOT/FOOTPAD (FP) : -ABRASION(S)/ULCERATION(S); LEFT HINDFOOT: SINGLE DRY BROWN ABRADED AREA, 4 MM IN DIAMETER; COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -ANIMAL OBESE SALIV GL, MANDIB (SG) : >NOTE:>SEE SKIN FOR DIAGNOSIS OF MASS SKIN (SK) : -B-FIBROMA,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MANDIBULAR (MN) : -HYPERPLASIA, LYMPHOPLASMACYTIC,-SLIGHT LN, LUMBAR/ILIAC (LM) : -LYMPHANGIECTASIS,-PRESENT -HYPERPLASIA, LYMPHOPLASMACYTIC,-SLIGHT FOOT/FOOTPAD (FP) : -INFLAMMATION, CHRONIC-ACTIVE,MODERATELY SEVERE -ULCERATION,-MODERATE -ACANTHOSIS / HYPERKERATOSIS,-MODERATE 2323 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 571 ANIMAL NUMBER: C92690 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 1034.6 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 10:28 PROSECTOR: DAVID SCHUETTE RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PARATHYROID (PT), URINARY BLADDER (UB) 2324 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 572 ANIMAL NUMBER: C92691 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 736.0 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 8:38 PROSECTOR: GREGORY CODDINGTON RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >EYES; DISCOLORED/LIGHT RED; EYE-RIGHT; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY LIVER (LI) : -RED FOCUS(I)/AREA(S); LEFT LATERAL LOBE: MULTIPLE DARK RED AREAS, UP TO 3 X 2 MM; COLLECTED ON ROUTINE SECTION GENERAL COMMENT (GC) : -BLOOD FILM TAKEN HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MODERATE -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL -DEGENERATION, CYSTIC,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MODERATE PANCREAS (PA) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -ATROPHY, ACINAR,-MINIMAL -B-ADENOMA, ISLET CELL,-NEOPLASM PRESENT THYROID (TY) : -HYPERPLASIA, C-CELL,-SLIGHT -DILATATION / CYST, FOLLICULAR,-PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2325 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 573 ANIMAL NUMBER: C92691 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 736.0 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 8:38 PROSECTOR: GREGORY CODDINGTON RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN GENERAL INFORMATION (XX) : >NOTE:>ANIMAL NECROPSIED OUT OF ORDER DUE TO MORIBUND CONDITION THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAr /i LIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: TESTIS (TE), LUNG (LU), PARATHYROID (PT), URINARY BLADDER (UB) 2326 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 574 ANIMAL NUMBER: C92692 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 04/19/99 STUDY DAY OF DEATH: 365 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 04/19/99 8:00 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: NOT OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) HISTOPATHOLOGY BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -CONGESTION,-PRESENT KIDNEY (KD) : -REGENERATION, TUBULAR EPITHELIUM,MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT MARROW, FEMUR (FM) : -HYPOCELLULAR, MARROW,-MODERATE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),SEVERE LUNG (LU) : -CONGESTION,-PRESENT HEART (HT) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FIBROSIS,-SLIGHT TRACHEA (TR) : -EXUDATE, LUMINAL,-PRESENT PARATHYROID (PT) : >TISSUE MISSING 2327 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 575 ANIMAL NUMBER: C92692 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 04/19/99 STUDY DAY OF DEATH: 365 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 04/19/99 8:00 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 9 MM IN DIAMETER, RED, DARK RED, TAN, GREY AND SOFT SKIN (SK) : -CRUSTED AREA(S); SCROTUM: SINGLE RED, DRY CRUSTED AREA, 14 X 10 MM (CAP 21) SEMINAL VESICLES (SV) : -LARGE; BILATERAL: BOTH ARE 35 X 16 X 4 MM MAMMARY, MALE (MM) : -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM (CAP 22) COAGULATING GL (CG) : -LARGE; LEFT: 25 X 6 X 4 MM (FOR COMPARISON, RIGHT IS 10 X 6 X 3 MM); COLLECTED INTACT WITH SEMINAL VESICLES GENERAL COMMENT (GC) : -MALOCCLUSION; BILATERAL, UPPER INCISORS -DISCHARGE-OCULAR; BILATERAL: RED PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL JEJUNUM (JE) : >AUTOLYTIC ILEUM (IL) : >AUTOLYTIC SKIN (SK) : -ACANTHOSIS/HYPERKERATOSIS,-MINIMAL SEMINAL VESICLES (SV) : -SECRETION, INCREASED,-PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT MAMMARY, MALE (MM) : -HYPERPLASIA, CYSTIC,-MODERATE -SECRETORY MATERIAL IN LUMINA,-PRESENT COAGULATING GL (CG) : -SECRETORY MATERIAL, INCREASED,-PRESENT 2328 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 576 ANIMAL NUMBER: C92692 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 04/19/99 STUDY DAY OF DEATH: 365 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 04/19/99 8:00 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), ESOPHAGUS (ES), THYROID (TY), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2329 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 577 ANIMAL NUMBER: C92693 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 711.8 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 8:31 PROSECTOR: TERRI L. WILLIAMS RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); DORSAL; WEEK 98; 40 X 40MM; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; DORSAL; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; BLACK SKIN; DORSAL; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY LIVER (LI) : -LIGHT FOCUS(I)/AREA(S); RIGHT MEDIAN LOBE NEAR JUNCTION OF LEFT MEDIAN LOBE: SINGLE TAN AREA, 4 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION -RED FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE DARK RED AREAS, UP TO 5 MM IN DIAMETER; SOME ARE SUNKEN WITH A STELLATE PATTERN; COLLECTED ON ROUTINE SECTIONS LUNG (LU) : -MASS(ES); ALL LOBES, DIFFUSE: MULTIPLE MASSES, UP TO 15 MM IN DIAMETER, ALL ARE RED, TAN, AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (TRACHEOBRONCHIAL) NOT IDENTIFIED HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL -LIPIDOSIS, SUBCAPSULAR (TENSION LIPIDOSIS),-MODERATELY SEVERE KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SLIGHT PANCREAS (PA) : -INFILTRATION, FAT, INTERSTITIAL,SLIGHT LUNG (LU) : -N-SARCOMA,-FROM SKIN 2330 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 578 ANIMAL NUMBER: C92693 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 711.8 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 8:31 PROSECTOR: TERRI L. WILLIAMS RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY SKIN (SK) : -MASS(ES); LUMBAR REGION: SINGLE MASS, 40 X 35 X 20 MM, RED, DARK RED, TAN, AND FIRM; SAME ON CUT SURFACE; OVERLYING SKIN HAS A SINGLE ERODED AREA, 15 MM IN DIAMETER; COLLECTED INTACT; REGIONAL LN (INGUINAL) NOT IDENTIFIED LN, MEDIASTINAL (ML) : -LARGE; UP TO 5 MM IN DIAMETER; APPEARS CYSTIC (CAP 22) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -ANIMAL OBESE THYROID (TY) : -DILATATION / CYST, FOLLICULAR,-PRESENT SKIN (SK) : -M-FIBROSARCOMA,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MEDIASTINAL (ML) : -HYPERPLASIA, LYMPHOPLASMACYTIC,-SLIGHT -LYMPHANGIECTASIS,-SLIGHT 2331 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 579 ANIMAL NUMBER: C92693 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 711.8 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 8:31 PROSECTOR: TERRI L. WILLIAMS RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUm /s KULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: TESTIS (TE), PARATHYROID (PT), URINARY BLADDER (UB) 2332 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 580 ANIMAL NUMBER: C92694 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 918.8 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 13:04 PROSECTOR: JARED FISHEL RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; SORE/SCAB; PAW-HINDRIGHT; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-SLIGHT -VACUOLATION, HEPATOCELLULAR, MIDZONAL / CENTRILOBULAR,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL -DEGENERATION, CYSTIC,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL PANCREAS (PA) : -M-CARCINOMA, ISLET CELL,-NEOPLASM PRESENT THYROID (TY) : -HYPERPLASIA, C-CELL,-SLIGHT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT FOOT/FOOTPAD (FP) : -INFLAMMATION, CHRONIC-ACTIVE,MODERATELY SEVERE 2333 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 581 ANIMAL NUMBER: C92694 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 918.8 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 13:04 PROSECTOR: JARED FISHEL RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY FOOT/FOOTPAD (FP) : -CRUSTED AREA(S); RIGHT HINDFOOT: SINGLE DARK RED AND DRY CRUSTED AREA, 4 MM IN DIAMETER; COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -ANIMAL OBESE FOOT/FOOTPAD (FP) : -ULCERATION,-SLIGHT -ACANTHOSIS / HYPERKERATOSIS,-MODERATE 2334 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 582 ANIMAL NUMBER: C92694 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 918.8 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 13:04 PROSECTOR: JARED FISHEL RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: TESTIS (TE), LUNG (LU), PARATHYROID (PT), URINARY BLADDER (UB) 2335 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 583 ANIMAL NUMBER: C92695 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 727.2 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 8:21 PROSECTOR: JARED FISHEL RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY PITUITARY (PI) : -LARGE; 7 X 5 X 4 MM; APPEARS SWOLLEN HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-SLIGHT -VACUOLATION, HEPATOCELLULAR, MIDZONAL / CENTRILOBULAR,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-SLIGHT -DEGENERATION, CYSTIC,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL PARATHYROID (PT) : -HYPERPLASIA,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT -CYST,-PRESENT URINARY BLADDER (UB) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2336 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 584 ANIMAL NUMBER: C92695 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 727.2 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 8:21 PROSECTOR: JARED FISHEL RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY TRIGEMINAL NERVE (TG) : -LARGE; BILATERAL: EACH IS UP TO 4 MM IN DIAMETER; COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN TRIGEMINAL NERVE (TG) : -GANGLION,-PRESENT THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAr /i LIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), TESTIS (TE), THYROID (TY) 2337 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 585 ANIMAL NUMBER: C92696 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 785.1 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 12:51 PROSECTOR: GREGORY CODDINGTON RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; SORE/SCAB; PAW-HINDRIGHT; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-SLIGHT -VACUOLATION, HEPATOCELLULAR, MIDZONAL / CENTRILOBULAR,-SLIGHT -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -DEGENERATION, CYSTIC,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, PELVIC,-MINIMAL PANCREAS (PA) : -INFILTRATION, FAT, INTERSTITIAL,SLIGHT LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT 2338 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 586 ANIMAL NUMBER: C92696 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 785.1 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 12:51 PROSECTOR: GREGORY CODDINGTON RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -RAISED AREA(S); LEFT POSTERIOR ASPECT: SINGLE TAN AREA, 2 MM IN DIAMETER FOOT/FOOTPAD (FP) : -ABRASION(S)/ULCERATION(S); RIGHT HINDFOOT: SINGLE DARK RED AND TAN ABRADED AREA, 9 MM IN DIAMETER; COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN PITUITARY (PI) : -HYPERPLASIA,-MINIMAL URINARY BLADDER (UB) : -PROTEINACEOUS MATERIAL, LUMINAL,PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT FOOT/FOOTPAD (FP) : -INFLAMMATION, CHRONIC-ACTIVE,MODERATELY SEVERE -ULCERATION,-MODERATE -ACANTHOSIS / HYPERKERATOSIS,-MODERATE -BACTERIA,-PRESENT 2339 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 587 ANIMAL NUMBER: C92696 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 785.1 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 12:51 PROSECTOR: GREGORY CODDINGTON RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAr /i LIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: TESTIS (TE), PARATHYROID (PT) 2340 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 588 ANIMAL NUMBER: C92697 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/28/99 STUDY DAY OF DEATH: 465 STUDY WEEK OF DEATH: 67 TERMINAL BODY WEIGHT: 523.3 GRAMS DATE AND TIME OF NECROPSY: 07/28/99 7:35 PROSECTOR: JILL PAUS RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); DORSAL; WEEK 58; 15 X 15MM; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; DORSALTHORACIC; VERIFIED AT NECROPSY: NOT OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: NOT OBSERVED >EXCRETION; NO FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; RECUMBENT; LATERAL; VERIFIED AT NECROPSY: NOT APPLICABLE >EYES; RED DISCHARGE; EYES; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) HISTOPATHOLOGY BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT -MINERALIZATION,-MINIMAL LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MINIMAL PANCREAS (PA) : -B-ADENOMA, ISLET CELL,-NEOPLASM PRESENT ADRENAL, CORTEX (AC) : -PIGMENT,-MINIMAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),SEVERE LUNG (LU) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL 2341 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 589 ANIMAL NUMBER: C92697 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/28/99 STUDY DAY OF DEATH: 465 STUDY WEEK OF DEATH: 67 TERMINAL BODY WEIGHT: 523.3 GRAMS DATE AND TIME OF NECROPSY: 07/28/99 7:35 PROSECTOR: JILL PAUS RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 11 X 8 X 8 MM, RED, DARK RED, GREY, AND SOFT MAMMARY, MALE (MM) : -MASS(ES); LEFT DORSAL LUMBAR REGION: SINGLE MASS, 20 X 15 X 15 MM, RED, DARK RED, TAN, AND FIRM; SAME ON CUT SURFACE (CAP 21); REGIONAL LN (INGUINAL) NOT IDENTIFIED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: RED DRY MATERIAL -STAINS - SKIN/HAIRCOAT; ENTIRE VENTRAL SURFACE: BROWN DRY MATERIAL HEART (HT) : -CARDIOMYOPATHY,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT SKIN (SK) : -B-KERATOACANTHOMA,-NEOPLASM PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT MAMMARY, MALE (MM) : >UNREMARKABLE >NOTE:>SEE SKIN FOR DIAGNOSIS 2342 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 590 ANIMAL NUMBER: C92697 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/28/99 STUDY DAY OF DEATH: 465 STUDY WEEK OF DEATH: 67 TERMINAL BODY WEIGHT: 523.3 GRAMS DATE AND TIME OF NECROPSY: 07/28/99 7:35 PROSECTOR: JILL PAUS RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUm /s KULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), KIDNEY (KD), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2343 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 591 ANIMAL NUMBER: C92698 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 741.9 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 10:30 PROSECTOR: TONY HANFELD RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >WART LIKE LESION(S); AXILLARY REGIONLEFT; WEEK 82; VERIFIED AT NECROPSY: NOT OBSERVED >SKIN & PELAGE; SORE/SCAB; AXILLARY REGION-LEFT; VERIFIED AT NECROPSY: NOT OBSERVED >SKIN & PELAGE; BROWN HAIR COAT; PERINEAL AREA; VERIFIED AT NECROPSY: OBSERVED >EYES; PALE; EYES; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; OTHER; BLACK HAIR COAT PERINEAL; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >EXCRETION; LIQUID FECES; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY LIVER (LI) : -MASS(ES); ALL LOBES: MULTIPLE MASSES, UP 23 X 20 X 8 MM, ALL ARE TAN, BROWN, DARK RED AND FIRM; ALL ARE SAME ON CUT SURFACE; COLLECTED ON ROUTINE SECTIONS; REGIONAL LN (SEE HEPATIC) PANCREAS (PA) : -MASS(ES); INVOLVING ENTIRE ORGAN: SINGLE MASS, 45 X 40 X 35 MM, DARK RED AND TAN; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (SEE PANCREATIC) HISTOPATHOLOGY LIVER (LI) : -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL -N-CARCINOMA,-FROM PANCREAS KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MODERATELY SEVERE PANCREAS (PA) : -M-CARCINOMA, ISLET CELL,-NEOPLASM PRESENT TESTIS (TE) : -MINERALIZATION, VASCULAR,-MINIMAL LUNG (LU) : -N-CARCINOMA,-FROM PANCREAS THYROID (TY) : -HYPERPLASIA, C-CELL,-SLIGHT URINARY BLADDER (UB) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERPLASIA, TRANSITIONAL CELL,MINIMAL 2344 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 592 ANIMAL NUMBER: C92698 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 741.9 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 10:30 PROSECTOR: TONY HANFELD RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY LN, OTHER (LN) : -LARGE; HEPATIC: UP TO 7 X 3 X 3 MM (CAP 22) LN, ANT MES/PANC (AP) : -DIFFUSELY RED; PANCREATIC: DARK RED (CAP 23) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -STAINS-PERINEUM/PERIANAL; DRY BROWN MATERIAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, OTHER (LN) : >TISSUE MISSING LN, ANT MES/PANC (AP) : >TISSUE MISSING >NOTE:>NO LYMPH NODE; SEE PANCREAS 2345 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 593 ANIMAL NUMBER: C92698 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 741.9 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 10:30 PROSECTOR: TONY HANFELD RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUm /s KULL (CA), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PARATHYROID (PT) 2346 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 594 ANIMAL NUMBER: C92699 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 636.6 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 10:52 PROSECTOR: GREGORY CODDINGTON RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: NOT OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) TESTIS (TE) : -RED/YELLOW FOCUS(I)/AREA(S); RIGHT, BENEATH TUNICA: SINGLE YELLOW AREA, 11 X 10 MM HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL PANCREAS (PA) : -ATROPHY, ACINAR,-MINIMAL -INFILTRATION, FAT, INTERSTITIAL,MINIMAL TESTIS (TE) : -ATROPHY/DEGENERATION,-SEVERE -B-INTERSTITIAL CELL TUMOR,-NEOPLASM PRESENT >NOTE:>UNILATERAL 2347 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 595 ANIMAL NUMBER: C92699 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 636.6 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 10:52 PROSECTOR: GREGORY CODDINGTON RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 8 X 7 X 5 MM, RED, TAN, GREY AND SOFT TAIL (TI) : -CRUSTED AREA(S); PROXIMAL ONE THIRD: MULTIPLE BROWN AND DRY AREAS, UP TO 2 MM IN DIAMETER; COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT TAIL (TI) : -B-KERATOACANTHOMA,-NEOPLASM PRESENT 2348 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 596 ANIMAL NUMBER: C92699 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 636.6 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 10:52 PROSECTOR: GREGORY CODDINGTON RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), THYROID (TY), PARATHYROID (PT), URINARY BLADDER (UB) 2349 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 597 ANIMAL NUMBER: C92700 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 532.2 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 11:24 PROSECTOR: TROY ELLENBECKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; SWOLLEN; VENTRAL ABDOMINAL; VERIFIED AT NECROPSY: NOT OBSERVED >MASS(ES); AXILLARY REGION-LEFT; WEEK 98; 15 X 15MM; VERIFIED AT NECROPSY: NOT OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-MINIMAL -VACUOLATION, HEPATOCELLULAR, MIDZONAL / CENTRILOBULAR,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL -DEGENERATION, CYSTIC,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL PANCREAS (PA) : -INFILTRATION, FAT, INTERSTITIAL,SLIGHT -INFLAMMATION, VASCULAR,-MODERATE THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PARATHYROID (PT) : -B-ADENOMA,-NEOPLASM PRESENT 2350 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 598 ANIMAL NUMBER: C92700 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 532.2 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 11:24 PROSECTOR: TROY ELLENBECKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 10 X 7 X 5 MM, RED, TAN, AND SOFT PROSTATE (PR) : -NODULAR; PROXIMAL SURROUNDING URETHRA: GREEN AND FIRM GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT URINARY BLADDER (UB) : -PROTEINACEOUS MATERIAL, LUMINAL,PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2351 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 599 ANIMAL NUMBER: C92700 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 532.2 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 11:24 PROSECTOR: TROY ELLENBECKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUm /s KULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: TESTIS (TE), LUNG (LU) 2352 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 600 ANIMAL NUMBER: C92701 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/19/99 STUDY DAY OF DEATH: 365 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 555.8 GRAMS DATE AND TIME OF NECROPSY: 04/19/99 15:44 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); LIMB-FRONT-RIGHT; WEEK 47; 70 X 80MM; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; LIMB-FRONTRIGHT; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; HUNCHED; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; PALE; ENTIRE BODY; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; COLD TO TOUCH; ENTIRE BODY; VERIFIED AT NECROPSY: NOT APPLICABLE >EYES; RED DISCHARGE; EYE-RIGHT; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY HISTOPATHOLOGY LIVER (LI) : -NECROSIS, INDIVIDUAL HEPATOCYTE,MINIMAL -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, PERIPORTAL,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MODERATE -MINERALIZATION, PELVIC,-MINIMAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-MODERATE ADRENAL, MEDULLA (MA) : -UNILATERALLY EXAMINED,-PRESENT LN, MESENTERIC (MS) : -HEMORRHAGE,-PRESENT MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,MODERATELY SEVERE 2353 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 601 ANIMAL NUMBER: C92701 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/19/99 STUDY DAY OF DEATH: 365 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 555.8 GRAMS DATE AND TIME OF NECROPSY: 04/19/99 15:44 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY MUSCLE, SKELETAL (SM) : -MASS(ES); SURROUNDING ENTIRE FRONT RIGHT FORELIMB: SINGLE MASS,95 X 80 X 45 MM, TAN, RED, FIRM, AND LOBULATED; SAME ON CUT SURFACE; OVERLYING SKIN HAS A SINGLE DRY RED CRUSTED AREA, 60 X 55 MM; COLLECTED INTACT WITH MASS; REGIONAL LN (SEE AXILLARY) LN, AXILLARY (AX) : -LARGE; BILATERAL: EACH IS 7 X 4 X 2 MM (CAP 21) MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,MODERATELY SEVERE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATELY SEVERE MUSCLE, SKELETAL (SM) : -M-FIBROSARCOMA,-NEOPLASM PRESENT -INFLAMMATION, CHRONIC-ACTIVE,-SEVERE -BACTERIAL COLONIES,-PRESENT ESOPHAGUS (ES) : -HYPERKERATOSIS,-MODERATE PITUITARY (PI) : -HYPERPLASIA,-MINIMAL PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE EPIDIDYMIDES (EP) : -IMMATURE SPERM FORMS,-MINIMAL CAUSE OF DEATH (CD) : -NEOPLASM, SKIN/MUSCLE/SUBCUTIS,PRESENT LN, AXILLARY (AX) : -HEMORRHAGE,-PRESENT 2354 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 602 ANIMAL NUMBER: C92701 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/19/99 STUDY DAY OF DEATH: 365 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 555.8 GRAMS DATE AND TIME OF NECROPSY: 04/19/99 15:44 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY LN, AXILLARY (AX) : -DIFFUSELY RED; BILATERAL: DARK RED GENERAL COMMENT (GC) -BLOOD FILM TAKEN -DISCHARGE-OCULAR; MATERIAL -ANIMAL THIN : BILATERAL: DRY RED LN, AXILLARY (AX) : -HYPERPLASIA, LYMPHOPLASMACYTIC,MODERATE 2355 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 603 ANIMAL NUMBER: C92701 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/19/99 STUDY DAY OF DEATH: 365 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 555.8 GRAMS DATE AND TIME OF NECROPSY: 04/19/99 15:44 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), ADRENAL, CORTEX (AC), TESTIS (TE), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), NERVE, SCIATIC (SN), TRACHEA (TR), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), HEMATO NEOPLASIA (HN) 2356 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 604 ANIMAL NUMBER: C92702 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 601.7 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 10:10 PROSECTOR: JAMES T. CALLEN RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; HEAD TILT; LEFT; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -LIGHT FOCUS(I)/AREA(S); DIAPHRAGMATIC SURFACE OF LEFT LATERAL LOBE: SINGLE TAN AREA, 2 X 1 MM; COLLECTED ON ROUTINE SECTION PITUITARY (PI) : -MASS(ES); 12 X 7 X 5 MM, RED, DARK RED, TAN AND FIRM HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MODERATELY SEVERE -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-SLIGHT -FIBROSIS, PERIBILIARY,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SLIGHT PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-SLIGHT LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : -EROSION,-SLIGHT 2357 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 605 ANIMAL NUMBER: C92702 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 601.7 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 10:10 PROSECTOR: JAMES T. CALLEN RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: MULTIPLE DARK BROWN AREAS, UP TO 4 X 2 MM GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN URINARY BLADDER (UB) : -PROTEINACEOUS MATERIAL, LUMINAL,PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2358 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 606 ANIMAL NUMBER: C92702 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 601.7 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 10:10 PROSECTOR: JAMES T. CALLEN RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: TESTIS (TE), PARATHYROID (PT) 2359 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 607 ANIMAL NUMBER: C92703 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 06/16/99 STUDY DAY OF DEATH: 423 STUDY WEEK OF DEATH: 61 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 06/16/99 7:48 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: NOT OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); SINGLE MASS, 11 X 9 X 6 MM DARK RED AND SOFT HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATELY SEVERE PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL ADRENAL, CORTEX (AC) : -PIGMENT,-MINIMAL THYMUS (TH) : -HEMORRHAGE,-PRESENT -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATE HEART (HT) : -CARDIOMYOPATHY,-SLIGHT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT 2360 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 608 ANIMAL NUMBER: C92703 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 06/16/99 STUDY DAY OF DEATH: 423 STUDY WEEK OF DEATH: 61 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 06/16/99 7:48 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -DISCHARGE-OCULAR; BILATERAL: DARK RED -DISCHARGE-NASAL; DARK RED JEJUNUM (JE) : >AUTOLYTIC PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 2361 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 609 ANIMAL NUMBER: C92703 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 06/16/99 STUDY DAY OF DEATH: 423 STUDY WEEK OF DEATH: 61 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 06/16/99 7:48 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2362 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 610 ANIMAL NUMBER: C92704 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 860.3 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 12:35 PROSECTOR: JARED FISHEL RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK 90; 20 X 20MM; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LUNG (LU) : >NOTE:>TRIMMING COMMENT: MISSING FROM WET TISSUE AT TRIMMING TRACHEA (TR) : >NOTE:>TRIMMING COMMENT: MISSING FROM WET TISSUE AT TRIMMING ESOPHAGUS (ES) : >NOTE:>TRIMMING COMMENT: MISSING FROM WET TISSUE AT TRIMMING HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL -FIBROSIS, PERIBILIARY,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SLIGHT PANCREAS (PA) : -ATROPHY, ACINAR,-MINIMAL LUNG (LU) : >TISSUE MISSING TRACHEA (TR) : >NOT REQUIRED TO BE EXAMINED FOR ANIMAL ESOPHAGUS (ES) : >NOT REQUIRED TO BE EXAMINED FOR ANIMAL 2363 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 611 ANIMAL NUMBER: C92704 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 860.3 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 12:35 PROSECTOR: JARED FISHEL RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY THYROID (TY) : >NOTE:>TRIMMING COMMENT: MISSING FROM WET TISSUE AT TRIMMING PARATHYROID (PT) : >NOTE:>TRIMMING COMMENT: MISSING FROM WET TISSUE AT TRIMMING SKIN (SK) : -MASS(ES); LEFT LATERAL ABDOMINAL REGION: SINGLE MASS, 25 MM IN DIAMETER, TAN AND FIRM; ON CUT SURFACE EXUDES A TAN, CASEOUS SEMISOLID; COLLECTED; REGIONAL LN (AXILLARY) NORMAL (CAP 22); OVERLYING SKIN HAS A SINGLE DRY, TAN ULCERATED AREA, 8 MM IN DIAMETER; COLLECTED INTACT WITH MASS GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN THYROID (TY) : >TISSUE MISSING PARATHYROID (PT) : >TISSUE MISSING SKIN (SK) : -B-KERATOACANTHOMA,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2364 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 612 ANIMAL NUMBER: C92704 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 860.3 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 12:35 PROSECTOR: JARED FISHEL RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: TESTIS (TE), URINARY BLADDER (UB), LN, AXILLARY (AX) 2365 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 613 ANIMAL NUMBER: C92705 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 852.5 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 13:23 PROSECTOR: CHANESTA ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >EYES; DISCOLORED/LIGHT RED; EYE-RIGHT; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY LIVER (LI) : -LIGHT FOCUS(I)/AREA(S); LEFT MEDIAN LOBE: MULTIPLE TAN FOCI, UP TO 2 MM IN DIAMETER; COLLECTED HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-MODERATE -VACUOLATION, HEPATOCELLULAR, MIDZONAL / CENTRILOBULAR,-SLIGHT -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MODERATELY SEVERE -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MODERATE KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL PANCREAS (PA) : -ATROPHY, ACINAR,-SLIGHT -INFILTRATION, FAT, INTERSTITIAL,SLIGHT LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT 2366 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 614 ANIMAL NUMBER: C92705 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 852.5 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 13:23 PROSECTOR: CHANESTA ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY THYROID (TY) : -LARGE; LEFT: 6 X 4 X 3 MM (RIGHT IS 4 X 3 X 2 MM) MAMMARY, MALE (MM) : -MASS(ES); LEFT LATERAL THORACIC REGION: SINGLE MASS, 20 X 15 X 7 MM, TAN, FIRM AND LOBULATED; SAME ON CUT SURFACE (CAP 23); REGIONAL LN (AXILLARY) NOT IDENTIFIED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT MAMMARY, MALE (MM) : -B-FIBROADENOMA,-NEOPLASM PRESENT 2367 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 615 ANIMAL NUMBER: C92705 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 852.5 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 13:23 PROSECTOR: CHANESTA ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUm /s KULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: TESTIS (TE), PARATHYROID (PT), URINARY BLADDER (UB) 2368 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 616 ANIMAL NUMBER: C92706 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/28/00 STUDY DAY OF DEATH: 680 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: 789.7 GRAMS DATE AND TIME OF NECROPSY: 02/28/00 14:42 PROSECTOR: TERRI L. WILLIAMS RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >EYES; RED DISCHARGE; EYES; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; LIMITED USE; LIMBS-HIND; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) HISTOPATHOLOGY BRAIN (BR) : -M-ASTROCYTOMA,-NEOPLASM PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL -B-ADENOMA, HEPATOCELLULAR,-NEOPLASM PRESENT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SLIGHT -MINERALIZATION, PELVIC,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-SEVERE -INFILTRATION, FAT, INTERSTITIAL,MINIMAL ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-SLIGHT -PIGMENT,-MINIMAL ADRENAL, MEDULLA (MA) : -B-PHEOCHROMOCYTOMA,-NEOPLASM PRESENT 2369 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 617 ANIMAL NUMBER: C92706 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/28/00 STUDY DAY OF DEATH: 680 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: 789.7 GRAMS DATE AND TIME OF NECROPSY: 02/28/00 14:42 PROSECTOR: TERRI L. WILLIAMS RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 9 X 8 X 6 MM, RED, TAN, BROWN, AND SOFT STOMACH, GL (ST) : -THICKENED WALL; DIFFUSE GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DARK RED -DISCHARGE-NASAL; BILATERAL: RED -ANIMAL OBESE -DISCHARGE-ORAL; CLEAR THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),SEVERE HEART (HT) : -CARDIOMYOPATHY,-MINIMAL MUSCLE, SKELETAL (SM) : -ATROPHY,-MODERATE NERVE, SCIATIC (SN) : -DEGENERATION,-SLIGHT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : -HYPERPLASIA, EPITHELIAL, GLANDULAR,SLIGHT URINARY BLADDER (UB) : -PROTEINACEOUS MATERIAL, LUMINAL,PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, BRAIN,-PRESENT 2370 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 618 ANIMAL NUMBER: C92706 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/28/00 STUDY DAY OF DEATH: 680 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: 789.7 GRAMS DATE AND TIME OF NECROPSY: 02/28/00 14:42 PROSECTOR: TERRI L. WILLIAMS RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUm /s KULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2371 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 619 ANIMAL NUMBER: C92707 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/06/00 STUDY DAY OF DEATH: 627 STUDY WEEK OF DEATH: 90 TERMINAL BODY WEIGHT: 485.2 GRAMS DATE AND TIME OF NECROPSY: 01/06/00 14:55 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; THIN; VERIFIED AT NECROPSY: NOT OBSERVED >SKIN & PELAGE; ROUGH HAIR COAT; VERIFIED AT NECROPSY: NOT APPLICABLE >DISCHARGE; NASAL; RED IN COLOR; VERIFIED AT NECROPSY: OBSERVED >EYES; SQUINTED; PERI-ORBITAL; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -MOTTLED; ALL LOBES: TAN AND BROWN KIDNEY (KD) : -LARGE PELVIS(ES); BILATERAL -LIGHT FOCUS(I)/AREA(S); RIGHT CORTICAL SURFACE: MULTIPLE TAN AREAS, UP TO 1 MM IN DIAMETER HISTOPATHOLOGY BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-MODERATELY SEVERE -CYST, BILIARY,-PRESENT KIDNEY (KD) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE -BACTERIAL COLONIES,-PRESENT SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-MODERATE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATELY SEVERE HEART (HT) : -CARDIOMYOPATHY,-MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-SLIGHT 2372 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 620 ANIMAL NUMBER: C92707 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/06/00 STUDY DAY OF DEATH: 627 STUDY WEEK OF DEATH: 90 TERMINAL BODY WEIGHT: 485.2 GRAMS DATE AND TIME OF NECROPSY: 01/06/00 14:55 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 15 X 11 X 10 MM, DARK RED, TAN, AND SOFT STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA: MULTIPLE TAN ERODED AREAS, UP TO 3 MM IN DIAMETER PROSTATE (PR) : -NODULAR; DIFFUSE: GREEN AND FIRM LN, LUMBAR/ILIAC (LM) : -MOTTLED; BILATERAL: DARK RED AND TAN (CAP 23) CAVITY, ABDOM (PC) : -MASS(ES); ATTACHED TO ADIPOSE TISSUE CRANIAL TO LEFT TESTIS: SINGLE MASS, 6 X 5 X 5 MM, YELLOW AND FIRM; SAME ON CUT SURFACE (CAP 22); REGIONAL LN (SEE LUMBAR) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL STOMACH, NONGL (SU) : -ACANTHOSIS / HYPERKERATOSIS,MODERATELY SEVERE -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,MODERATELY SEVERE SEMINAL VESICLES (SV) : -ATROPHY,-MODERATE CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT LN, LUMBAR/ILIAC (LM) : -HYPERPLASIA, LYMPHOPLASMACYTIC,MODERATE -HEMORRHAGE,-PRESENT CAVITY, ABDOM (PC) : -INFLAMMATION / NECROSIS, FAT,-SLIGHT 2373 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 621 ANIMAL NUMBER: C92707 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/06/00 STUDY DAY OF DEATH: 627 STUDY WEEK OF DEATH: 90 TERMINAL BODY WEIGHT: 485.2 GRAMS DATE AND TIME OF NECROPSY: 01/06/00 14:55 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -DISCHARGE-OCULAR; BILATERAL: DARK RED AND MOIST -DISCHARGE-NASAL; DARK RED AND DRY -URINARY BLADDER-LARGE LUMEN; 25 X 20 X 20 MM; CONTAINS A GREEN FLUID 2374 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 622 ANIMAL NUMBER: C92707 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/06/00 STUDY DAY OF DEATH: 627 STUDY WEEK OF DEATH: 90 TERMINAL BODY WEIGHT: 485.2 GRAMS DATE AND TIME OF NECROPSY: 01/06/00 14:55 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2375 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 623 ANIMAL NUMBER: C92708 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 724.1 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 9:44 PROSECTOR: JARED FISHEL RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -MALOCCLUSION; BILATERAL UPPER INCISORS HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-SLIGHT -DEGENERATION, CYSTIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SLIGHT PANCREAS (PA) : -ECTOPIC HEPATOCYTES,-PRESENT TESTIS (TE) : -B-INTERSTITIAL CELL TUMOR,-NEOPLASM PRESENT THYROID (TY) : -HYPERPLASIA, C-CELL,-SLIGHT -B-ADENOMA, FOLLICULAR CELL,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2376 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 624 ANIMAL NUMBER: C92708 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 724.1 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 9:44 PROSECTOR: JARED FISHEL RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -DISCHARGE-OCULAR; RIGHT: DRY RED MATERIAL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PARATHYROID (PT), URINARY BLADDER (UB) 2377 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 625 ANIMAL NUMBER: C92709 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 01/12/00 STUDY DAY OF DEATH: 633 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 01/12/00 9:38 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; PALE; ENTIRE BODY; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) KIDNEY (KD) : -CYST(S); TRIMMING COMMENT: BILATERAL, DIFFUSE: MULTIPLE CLEAR FLUID FILLED CYSTS, UP TO 1 MM IN DIAMETER -LARGE PELVIS(ES); BILATERAL -ROUGH SURFACE, DIFFUSE; BILATERAL PANCREAS (PA) : -LARGE VESSELS; DIFFUSE: UP TO 6 MM IN DIAMETER (CAP 23) TESTIS (TE) : -SMALL; BILATERAL: LEFT IS 16 X 11 X 5 MM, RIGHT IS 20 X 12 X 6 MM; BOTH ARE SOFT HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -VACUOLATION, HEPATOCELLULAR, MIDZONAL / CENTRILOBULAR,-SLIGHT -NECROSIS, COAGULATIVE,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SEVERE SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATELY SEVERE PANCREAS (PA) : -INFLAMMATION, VASCULAR,-SEVERE ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MINIMAL -PIGMENT,-MINIMAL TESTIS (TE) : -ATROPHY/DEGENERATION,-SLIGHT -INFLAMMATION, VASCULAR,-SLIGHT 2378 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 626 ANIMAL NUMBER: C92709 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 01/12/00 STUDY DAY OF DEATH: 633 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 01/12/00 9:38 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES) ; 7 X 6 X 4 MM, TAN, GRAY AND SOFT STOMACH, GL (ST) : -THICKENED WALL; DIFFUSE MARROW, FEMUR (FM) : -HYPERPLASIA, ERYTHROID, MARROW,MODERATELY SEVERE MARROW, STERNUM (SE) : -HYPERPLASIA, ERYTHROID, MARROW,MODERATELY SEVERE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATE LUNG (LU) : -MINERALIZATION, SEPTAL,-MINIMAL HEART (HT) : -CARDIOMYOPATHY,-SLIGHT -MINERALIZATION, VASCULAR,-MODERATELY SEVERE PARATHYROID (PT) : -HYPERPLASIA,-MODERATE PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : -MINERALIZATION, GLANDULAR MUCOSA,MODERATELY SEVERE CECUM (CE) : >AUTOLYTIC COLON (CO) : >AUTOLYTIC 2379 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 627 ANIMAL NUMBER: C92709 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 01/12/00 STUDY DAY OF DEATH: 633 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 01/12/00 9:38 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY SEMINAL VESICLES (SV) : -SMALL; BILATERAL: EACH IS 15 X 6 X 2 MM AORTA (AO) : -LARGE; DIFFUSE: UP TO 6 MM IN DIAMETER; COLLECTED GENERAL COMMENT (GC) : -ANIMAL THIN -DISCHARGE-NASAL; DARK RED AND DRY PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL SEMINAL VESICLES (SV) : -ATROPHY,-SEVERE EPIDIDYMIDES (EP) : -ATROPHY/DEGENERATION,-MODERATE -VACUOLATION, TUBULAR EPITHELIUM,MODERATE CAUSE OF DEATH (CD) : -NEPHROPATHY,-PRESENT AORTA (AO) : -MINERALIZATION,-SLIGHT 2380 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 628 ANIMAL NUMBER: C92709 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 01/12/00 STUDY DAY OF DEATH: 633 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 01/12/00 9:38 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), SPLEEN (SP), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAr /i LIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), CALVARIUm /s KULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), HEMATO NEOPLASIA (HN) 2381 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 629 ANIMAL NUMBER: C92710 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 08/18/99 STUDY DAY OF DEATH: 486 STUDY WEEK OF DEATH: 70 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 08/18/99 15:15 PROSECTOR: DAVID SCHUETTE RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-SLIGHT -DEGENERATION, CYSTIC,-MINIMAL -NECROSIS, COAGULATIVE,-MINIMAL -VACUOLATION, HEPATOCELLULAR,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL PANCREAS (PA) : -HYPERPLASIA, ISLET CELLS,-MINIMAL -ATROPHY, ACINAR,-MINIMAL MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,-SLIGHT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MINIMAL HEART (HT) : -CARDIOMYOPATHY,-MINIMAL 2382 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 630 ANIMAL NUMBER: C92710 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 08/18/99 STUDY DAY OF DEATH: 486 STUDY WEEK OF DEATH: 70 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 08/18/99 15:15 PROSECTOR: DAVID SCHUETTE RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS PARATHYROID (PT) : >SECTION EXAMINED; TISSUE NOT PRESENT ILEUM (IL) : >AUTOLYTIC CECUM (CE) : >AUTOLYTIC COLON (CO) : >AUTOLYTIC CAUSE OF DEATH (CD) : -UNDETERMINED MICROSCOPICALLY,-PRESENT 2383 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 631 ANIMAL NUMBER: C92710 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 08/18/99 STUDY DAY OF DEATH: 486 STUDY WEEK OF DEATH: 70 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 08/18/99 15:15 PROSECTOR: DAVID SCHUETTE RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2384 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 632 ANIMAL NUMBER: C92711 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 01/19/00 STUDY DAY OF DEATH: 640 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 01/19/00 8:04 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) HISTOPATHOLOGY BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -HYPERPLASIA, BILE DUCT,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT ADRENAL, CORTEX (AC) : -HYPERTROPHY, CORTICAL CELL,-MINIMAL -HYPERPLASIA, CORTICAL,-MINIMAL -B-ADENOMA,-NEOPLASM PRESENT MARROW, FEMUR (FM) : -HYPOCELLULAR, MARROW,-MODERATE THYMUS (TH) : >TISSUE MISSING HEART (HT) : -CARDIOMYOPATHY,-SLIGHT ESOPHAGUS (ES) : -EXUDATE, LUMINAL,-PRESENT THYROID (TY) : -DILATATION / CYST, FOLLICULAR,-PRESENT 2385 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 633 ANIMAL NUMBER: C92711 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 01/19/00 STUDY DAY OF DEATH: 640 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 01/19/00 8:04 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 12 X 9 X 6 MM, DARK RED, TAN AND FIRM STOMACH, GL (ST) : -LIGHT FOCUS(I)/AREA(S); MUCOSA: SINGLE TAN SLIGHTLY RAISED AREA, 8 X 5 MM MAMMARY, MALE (MM) : -MASS(ES); LEFT INGUINAL REGION: SINGLE MASS, 30 X 20 X 15 MM, TAN AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (INGUINAL) NOT IDENTIFIED GENERAL COMMENT (GC) : -DISCHARGE-OCULAR; BILATERAL: DRY RED MATERIAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : -FIBROSIS,-SLIGHT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT MAMMARY, MALE (MM) : -B-FIBROADENOMA,-NEOPLASM PRESENT 2386 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 634 ANIMAL NUMBER: C92711 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 01/19/00 STUDY DAY OF DEATH: 640 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 01/19/00 8:04 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOt /f OOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2387 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 635 ANIMAL NUMBER: C92712 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/13/99 STUDY DAY OF DEATH: 603 STUDY WEEK OF DEATH: 87 TERMINAL BODY WEIGHT: 497.3 GRAMS DATE AND TIME OF NECROPSY: 12/13/99 8:32 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >EYES; SQUINTED; PERI-ORBITAL; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -LIGHT FOCUS(I)/AREA(S); LEFT LATERAL LOBE: SINGLE TAN AREA, 5 X 3 MM; COLLECTED ON ROUTINE SECTION >NOTE:>TRIMMING COMMENT: RIGHT MEDIAN LOBE OF LIVER MISSING AT TRIMMING KIDNEY (KD) : -LARGE PELVIS(ES); RIGHT HISTOPATHOLOGY BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-SLIGHT -NECROSIS, COAGULATIVE,-SLIGHT -INFILTRATE, MACROPHAGE, PIGMENTED,SLIGHT -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MODERATE SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SLIGHT PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-MODERATE -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL -B-ADENOMA, ISLET CELL,-NEOPLASM PRESENT -INFILTRATION, FAT, INTERSTITIAL,MINIMAL 2388 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 636 ANIMAL NUMBER: C92712 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/13/99 STUDY DAY OF DEATH: 603 STUDY WEEK OF DEATH: 87 TERMINAL BODY WEIGHT: 497.3 GRAMS DATE AND TIME OF NECROPSY: 12/13/99 8:32 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 10 X 9 X 7 MM, RED, RED, GREY, TAN AND SOFT PROSTATE (PR) : -GELATINOUS; CLEAR AND SOFT DARK LN, RENAL (RL) : -LARGE; LEFT: UP TO 4 X 4 X 3 MM (RIGHT IS UP TO 3 X 3 X 2 MM) (CAP 22) -DIFFUSELY RED; LEFT ADRENAL, CORTEX (AC) : -PIGMENT,-MINIMAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATELY SEVERE HEART (HT) : -CARDIOMYOPATHY,-MINIMAL THYROID (TY) : -DILATATION / CYST, FOLLICULAR,-PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SEVERE SEMINAL VESICLES (SV) : -ATROPHY,-SEVERE EPIDIDYMIDES (EP) : -VACUOLATION, TUBULAR EPITHELIUM,MODERATELY SEVERE CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT LN, RENAL (RL) : -HYPERPLASIA, LYMPHOPLASMACYTIC,MODERATE -HEMORRHAGE,-PRESENT -INFILTRATE, MACROPHAGE, PIGMENTED,SLIGHT 2389 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 637 ANIMAL NUMBER: C92712 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/13/99 STUDY DAY OF DEATH: 603 STUDY WEEK OF DEATH: 87 TERMINAL BODY WEIGHT: 497.3 GRAMS DATE AND TIME OF NECROPSY: 12/13/99 8:32 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) -BLOOD FILM TAKEN -DISCHARGE-OCULAR; MATERIAL -ANIMAL THIN : BILATERAL: DRY, RED THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), HEMATO NEOPLASIA (HN) 2390 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 638 ANIMAL NUMBER: C92713 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 540.6 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 9:38 PROSECTOR: DONIA MEYER RECORDER: KIMBERLY DURLAND POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT' (%) LF ADRENAL (AD0) RT ADRENAL (AD1) BRAIN (BR) LF KIDNEY (KD0) RT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LF TESTIS (TE0) RT TESTIS (TE1) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) .0305 .0304 2.2845 1.8515 1.8679 18.6941 2.0157 .6982 1.6468 1.6167 ..00111320 .0056 % .0056 % .4226 % .3425 % .3455 % 3.4580 % .3729 % .1292 % .3046 % .2991 % ..00002214 % % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0134 .0133 1.0000 .8105 .8176 8.1830 .8823 .3056 .7209 .7077 .0057 .0049 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-MINIMAL -NECROSIS, INDIVIDUAL HEPATOCYTE,MINIMAL -VACUOLATION, HEPATOCELLULAR, MIDZONAL / CENTRILOBULAR,-MINIMAL PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-SLIGHT -INFILTRATE, LYMPHOHISTIOCYTIC,-SLIGHT -HYPERPLASIA, ISLET CELLS,-SLIGHT 2391 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 639 ANIMAL NUMBER: C92713 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 540.6 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 9:38 PROSECTOR: DONIA MEYER RECORDER: KIMBERLY DURLAND POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE) 2392 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 640 ANIMAL NUMBER: C92714 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 533.8 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 13:29 PROSECTOR: MICHELE MARGGI RECORDER: MATTHEW AXTMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: MATTHEW AXTMAN ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT' (%) LF ADRENAL (AD0) RT ADRENAL (AD1) BRAIN (BR) LF KIDNEY (KD0) RT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LF TESTIS (TE0) RT TESTIS (TE1) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) .0325 .0386 2.4852 2.3377 2.2096 20.0992 5.3343 .6615 1.8935 1.9044 .0132 .0217 .0061 % .0072 % .4656 % .4379 % .4139 % 3.7653 % .9993 % .1239 % .3547 % .3568 % .0025 % .0041 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0131 .0155 1.0000 .9406 .8891 8.0876 2.1464 .2662 .7619 .7663 .0053 .0087 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-SLIGHT -VACUOLATION, HEPATOCELLULAR, MIDZONAL / CENTRILOBULAR,-SLIGHT KIDNEY (KD) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -REGENERATION, TUBULAR EPITHELIUM,MINIMAL PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-MINIMAL 2393 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 641 ANIMAL NUMBER: C92714 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 533.8 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 13:29 PROSECTOR: MICHELE MARGGI RECORDER: MATTHEW AXTMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: MATTHEW AXTMAN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN PANCREAS (PA) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE) 2394 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 642 ANIMAL NUMBER: C92715 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 323.7 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 12:06 PROSECTOR: JODI THIEREN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN STATUS LF ADRENAL (AD0) RT ADRENAL (AD1) BRAIN (BR) LF KIDNEY (KD0) RT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LF TESTIS (TE0) RT TESTIS (TE1) LF THYROID/PARA RT THYROID/PARA (TT0) (TT1) ------------------------------------ 11.7462 ------------------------------------------- ------------------------------------ 3.6290 % ------------------------------------------- ------------------------------------------------------------------------------------- NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN WEIGHT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS 2395 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 643 ANIMAL NUMBER: C92715 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 323.7 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 12:06 PROSECTOR: JODI THIEREN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) 2396 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 644 ANIMAL NUMBER: C92716 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 429.7 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 10:40 PROSECTOR: GREGORY CODDINGTON RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >EYES; DISCOLORED/LIGHT RED; EYES; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; THIN; VERIFIED AT NECROPSY: NOT OBSERVED >EXCRETION; NONFORMED FECES; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY TESTIS (TE) : -SMALL; BILATERAL: EACH IS 18 X 10 X 7 MM; BOTH ARE SOFT THYMUS (TH) : -MISSING/NOT IDENTIFIED; NOT POSITIVELY IDENTIFIED, POSSIBLE REMNANTS COLLECTED URINARY BLADDER (UB) : -THICKENED WALL; DIFFUSE PROSTATE (PR) : -NODULAR; DISTAL END: GREEN AND FIRM HISTOPATHOLOGY LIVER (LI) : -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SLIGHT -MINERALIZATION, TUBULAR,-MINIMAL -CYST,-PRESENT TESTIS (TE) : -ATROPHY/DEGENERATION,-SEVERE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATE LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL URINARY BLADDER (UB) : -HYPERPLASIA, TRANSITIONAL CELL,-SLIGHT -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SEVERE 2397 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 645 ANIMAL NUMBER: C92716 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 429.7 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 10:40 PROSECTOR: GREGORY CODDINGTON RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY LN, MEDIASTINAL (ML) : -CYST(S); SINGLE CLEAR FLUID FILLED CYST, 3 MM IN DIAMETER (CAP 22) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN PROSTATE (PR) : -HYPERPLASIA, EPITHELIAL,-MODERATE CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MEDIASTINAL (ML) : -LYMPHANGIECTASIS,-MODERATE 2398 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 646 ANIMAL NUMBER: C92716 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 429.7 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 10:40 PROSECTOR: GREGORY CODDINGTON RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), THYROID (TY), PARATHYROID (PT) 2399 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 647 ANIMAL NUMBER: C92717 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 599.1 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 10:44 PROSECTOR: TODD ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); VENTRAL-THORACIC-LEFT; WEEK 86; 30 X 30MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); DORSAL; WEEK 98; VERIFIED AT NECROPSY: NOT OBSERVED >SKIN & PELAGE; SORE/SCAB; DORSAL; VERIFIED AT NECROPSY: NOT OBSERVED OBSERVATIONS NECROPSY KIDNEY (KD) : -CYST(S); BILATERAL CORTICAL SURFACES: SINGLE CLEAR FLUID FILLED CYST ON EACH, LEFT IS 1 MM IN DIAMETER, RIGHT IS 2 MM IN DIAMETER -ROUGH SURFACE, DIFFUSE; BILATERAL -DIFFUSELY LIGHT; BILATERAL: TAN THYROID (TY) : -LARGE; TRIMMING COMMENT: BILATERAL: EACH IS 10 X 6 X 3 MM HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -DEGENERATION, CYSTIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-SLIGHT -FIBROSIS, PERIBILIARY,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SEVERE -CYST,-PRESENT -THROMBUS,-PRESENT PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-MODERATE -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL TESTIS (TE) : -INFLAMMATION, VASCULAR,-SLIGHT LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL THYROID (TY) : -DILATATION / CYST, FOLLICULAR,-PRESENT PARATHYROID (PT) : -HYPERPLASIA,-MODERATELY SEVERE 2400 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 648 ANIMAL NUMBER: C92717 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 599.1 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 10:44 PROSECTOR: TODD ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -RED FOCUS(I)/AREA(S); RIGHT ANTERIOR ASPECT, AND RIGHT POSTERIOR ASPECT: SINGLE DARK RED AREA ON EACH, BOTH ARE 2 MM IN DIAMETER MAMMARY, MALE (MM) : -MASS(ES); LEFT LATERAL THORACIC REGION: SINGLE MASS, 35 X 30 X 20 MM, TAN, FIRM, AND VASCULAR; SAME ON CUT SURFACE; REGIONAL LN (AXILLARY) NORMAL (CAP 22) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT -HYPERPLASIA,-SLIGHT SKIN (SK) : -B-FIBROMA,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT MAMMARY, MALE (MM) : >NOT REQUIRED TO BE EXAMINED FOR ANIMAL >NOTE:>SEE SKIN FOR DIAGNOSIS OF MASS 2401 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 649 ANIMAL NUMBER: C92717 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 599.1 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 10:44 PROSECTOR: TODD ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUm /s KULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: URINARY BLADDER (UB), LN, AXILLARY (AX) 2402 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 650 ANIMAL NUMBER: C92718 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 278.5 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 12:00 PROSECTOR: JODI THIEREN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN STATUS LF ADRENAL (AD0) RT ADRENAL (AD1) BRAIN (BR) LF KIDNEY (KD0) RT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LF TESTIS (TE0) RT TESTIS (TE1) LF THYROID/PARA RT THYROID/PARA (TT0) (TT1) ------------------------------------ 9.1215 ------------------------------------------- ------------------------------------ 3.2752 % ------------------------------------------- ------------------------------------------------------------------------------------- NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN WEIGHT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS 2403 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 651 ANIMAL NUMBER: C92718 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 278.5 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 12:00 PROSECTOR: JODI THIEREN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) 2404 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 652 ANIMAL NUMBER: C92719 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 461.2 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 13:00 PROSECTOR: MICHELE MARGGI RECORDER: MATTHEW AXTMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: MATTHEW AXTMAN ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) LF ADRENAL (AD0) RT ADRENAL (AD1) BRAIN (BR) LF KIDNEY (KD0) RT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LF TESTIS (TE0) RT TESTIS (TE1) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) .0301 .0255 2.2522 1.9914 2.0458 15.8391 1.7406 .6376 1.6425 1.6706 .0220 .0225 .0065 % .0055 % .4883 % .4318 % .4436 % 3.4343 % .3774 % .1382 % .3561 % .3622 % .0048 % .0049 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0134 .0113 1.0000 .8842 .9084 7.0327 .7728 .2831 .7293 .7418 ..00100908 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED KIDNEY (KD) : -LARGE PELVIS(ES); BILATERAL LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-MINIMAL KIDNEY (KD) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -DILATATION, PELVIC,-SLIGHT -REGENERATION, TUBULAR EPITHELIUM,MINIMAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2405 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 653 ANIMAL NUMBER: C92719 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 461.2 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 13:00 PROSECTOR: MICHELE MARGGI RECORDER: MATTHEW AXTMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: MATTHEW AXTMAN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAr /i LIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE) 2406 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 654 ANIMAL NUMBER: C92720 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/16/99 STUDY DAY OF DEATH: 576 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/16/99 13:21 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); VENTRAL-ABDOMINAL; WEEK 76; 140 X 130MM; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; VENTRALABDOMINAL-RIGHT; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -NECROSIS, INDIVIDUAL HEPATOCYTE,MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-MINIMAL -HYPERPLASIA, BILE DUCT,-SLIGHT -FIBROSIS, PERIBILIARY,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATELY SEVERE PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL ADRENAL, CORTEX (AC) : -PIGMENT,-MINIMAL MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,MODERATELY SEVERE MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,MODERATELY SEVERE 2407 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 655 ANIMAL NUMBER: C92720 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/16/99 STUDY DAY OF DEATH: 576 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/16/99 13:21 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY MAMMARY, MALE (MM) : -MASS(ES); RIGHT ABDOMINAL REGION: SINGLE MASS, 150 X 140 X 50 MM, RED, DARK RED, TAN, VASCULAR, AND FIRM; SOME AREAS EXUDE A DARK BROWN FLUID; PORTION COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED; OVERLYING SKIN OF MASS HAS A SINGLE DARK BROWN, DRY, CRUSTED AREA, 90 X 65 MM; COLLECTED WITH PORTION OF MASS CAVITY, ABDOM (PC) : -MASS(ES); ATTACHED TO ADIPOSE TISSUE SURROUNDING RIGHT TESTIS: SINGLE MASS, 13 X 5 X 4 MM, TAN AND FIRM; SAME ON CUT SURFACE; (CAP 22); REGIONAL LN (LUMBAR) NOT IDENTIFIED THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),SEVERE LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL SKIN (SK) : -M-FIBROSARCOMA,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, SKIN/MUSCLE/SUBCUTIS,PRESENT MAMMARY, MALE (MM) : >NOT REQUIRED TO BE EXAMINED FOR ANIMAL >NOTE:>SEE SKIN FOR DIAGNOSIS CAVITY, ABDOM (PC) : -INFLAMMATION / NECROSIS, FAT,-SLIGHT 2408 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 656 ANIMAL NUMBER: C92720 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/16/99 STUDY DAY OF DEATH: 576 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/16/99 13:21 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG), GENERAL COMMENT (GC) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2409 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 657 ANIMAL NUMBER: C92721 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/28/99 STUDY DAY OF DEATH: 557 STUDY WEEK OF DEATH: 80 TERMINAL BODY WEIGHT: 540.6 GRAMS DATE AND TIME OF NECROPSY: 10/28/99 8:30 PROSECTOR: JARED FISHEL RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; MISSING; TEETH; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >EXCRETION; NONFORMED FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; SENSITIVE TO TOUCH; VERIFIED AT NECROPSY: NOT APPLICABLE >EYES; SQUINTED; PERI-ORBITAL-RIGHT; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; RED HAIR COAT; HEADENTIRE; VERIFIED AT NECROPSY: NOT OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) KIDNEY (KD) : -ROUGH SURFACE, DIFFUSE; BILATERAL PANCREAS (PA) : -DIFFUSELY RED; RED HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-MODERATE KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,MODERATELY SEVERE -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL -ATROPHY, ACINAR,-MINIMAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),SEVERE HEART (HT) : -CARDIOMYOPATHY,-SLIGHT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT 2410 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 658 ANIMAL NUMBER: C92721 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/28/99 STUDY DAY OF DEATH: 557 STUDY WEEK OF DEATH: 80 TERMINAL BODY WEIGHT: 540.6 GRAMS DATE AND TIME OF NECROPSY: 10/28/99 8:30 PROSECTOR: JARED FISHEL RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 12 X 6 X 6 MM, DARK RED, TAN, SOFT, AND LOBULATED STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: MULTIPLE PINPOINT DARK BROWN AREAS -THICKENED WALL; MUCOSA: DIFFUSE STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA: SINGLE DARK BROWN ERODED AREA, 4 MM IN DIAMETER -THICKENED WALL; MUCOSA: DIFFUSE -DARK FOCUS(I)/AREA(S); MUCOSA: MULTIPLE PINPOINT DARK BROWN AREAS GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL, LOWER INCISORS -TEETH MISSING/BROKEN/WORN; BILATERAL, UPPER INCISORS: BROKEN OFF AT BASE HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL STOMACH, GL (ST) : -INFLAMMATION, ACUTE,-SLIGHT STOMACH, NONGL (SU) : -ACANTHOSIS / HYPERKERATOSIS,-MODERATE -ULCERATION,-MODERATE -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE URINARY BLADDER (UB) : -PROTEINACEOUS MATERIAL, LUMINAL,PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 2411 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 659 ANIMAL NUMBER: C92721 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/28/99 STUDY DAY OF DEATH: 557 STUDY WEEK OF DEATH: 80 TERMINAL BODY WEIGHT: 540.6 GRAMS DATE AND TIME OF NECROPSY: 10/28/99 8:30 PROSECTOR: JARED FISHEL RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -DISCHARGE-OCULAR; RIGHT: DRY RED MATERIAL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), SPLEEN (SP), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAr /i LIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), SEMINAL VESICLES(SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2412 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 660 ANIMAL NUMBER: C92722 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 504.7 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 11:30 PROSECTOR: LAURIE J. SCHULLER RECORDER: KIMBERLY DURLAND POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT' (%) LF ADRENAL (AD0) RT ADRENAL (AD1) BRAIN (BR) LF KIDNEY (KD0) RT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LF TESTIS (TE0) RT TESTIS (TE1) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) .0278 .0245 2.2279 1.7612 1.7928 16.2488 2.2034 .6357 1.5641 1.5630 .0102 .0107 .0055 % .0049 % .4414 % .3490 % .3552 % 3.2195 % .4366 % .1260 % .3099 % .3097 % .0020 % .0021 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0125 .0110 1.0000 .7905 .8047 7.2933 .9890 .2853 .7021 .7016 .0046 .0048 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED LN, MESENTERIC (MS) : >NOTE:>TRIMMING COMMENT: MESENTERIC LN MISSING FROM WET TISSUE AT TIME OF TRIMMING LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-MINIMAL KIDNEY (KD) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -REGENERATION, TUBULAR EPITHELIUM,MINIMAL LN, MESENTERIC (MS) : >TISSUE MISSING 2413 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 661 ANIMAL NUMBER: C92722 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 504.7 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 11:30 PROSECTOR: LAURIE J. SCHULLER RECORDER: KIMBERLY DURLAND POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), TESTIS (TE) 2414 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 662 ANIMAL NUMBER: C92723 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/23/99 STUDY DAY OF DEATH: 552 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: 685.3 GRAMS DATE AND TIME OF NECROPSY: 10/23/99 9:58 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >EYES; SQUINTED; PERI-ORBITAL-RIGHT; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; RED HAIR COAT; VENTRAL ABDOMINAL; VERIFIED AT NECROPSY: OBSERVED >EYES; RED DISCHARGE; EYES; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) HISTOPATHOLOGY BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-MODERATE -HYPERPLASIA, BILE DUCT,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SLIGHT -INFLAMMATION, SUPPURATIVE, PELVIC,MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL ADRENAL, CORTEX (AC) : -HYPERPLASIA, CORTICAL,-MINIMAL -PIGMENT,-MINIMAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATELY SEVERE 2415 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 663 ANIMAL NUMBER: C92723 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/23/99 STUDY DAY OF DEATH: 552 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: 685.3 GRAMS DATE AND TIME OF NECROPSY: 10/23/99 9:58 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 13 X 8 X 5 MM, DARK RED AND SOFT STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA: MULTIPLE REDDISH-TAN ERODED AREAS, UP TO 4 X 2 MM -THICKENED WALL; MUCOSA: DIFFUSE PROSTATE (PR) : -NODULAR; PROXIMAL, SURROUNDING URETHRA: GREEN AND FIRM SEMINAL VESICLES (SV) : -LARGE; BILATERAL: EACH IS 30 X 13 X 5 MM GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DARK RED AND CRUSTED HEART (HT) : -CARDIOMYOPATHY,-MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT SALIV GL, MANDIB (SG) : -HYPERPLASIA, GLANDULAR,-MINIMAL STOMACH, GL (ST) : -INFLAMMATION, ACUTE,-MINIMAL STOMACH, NONGL (SU) : -ACANTHOSIS / HYPERKERATOSIS,-MODERATE -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT SEMINAL VESICLES (SV) : -SECRETION, INCREASED,-PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 2416 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 664 ANIMAL NUMBER: C92723 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/23/99 STUDY DAY OF DEATH: 552 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: 685.3 GRAMS DATE AND TIME OF NECROPSY: 10/23/99 9:58 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -STAINS - SKIN/HAIRCOAT; ENTIRE VENTRAL SURFACE OF ANIMAL: DARK RED AND DRY THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2417 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 665 ANIMAL NUMBER: C92724 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 975.4 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 11:12 PROSECTOR: CHANESTA ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); VENTRAL-ABDOMINAL; WEEK 73; 10 X 10MM; VERIFIED AT NECROPSY: NOT OBSERVED >WART LIKE LESION(S); DORSAL; WEEK 96; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; VENTRAL ABDOMINAL; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY PITUITARY (PI) : -RED FOCUS(I)/AREA(S); RIGHT POSTERIOR ASPECT: SINGLE DARK RED AREA, 1 MM IN DIAMETER SKIN (SK) : -CRUSTED AREA(S); MID VENTRAL ABDOMINAL AREA: SINGLE TAN, RED, DARK RED AND DRY CRUSTED AREA, 10 X 8 MM; (CAP 22) HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SLIGHT PANCREAS (PA) : -INFILTRATION, FAT, INTERSTITIAL,MODERATE THYROID (TY) : -DILATATION / CYST, FOLLICULAR,-PRESENT -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT SKIN (SK) : -B-KERATOACANTHOMA,- 2 NEOPLASMS PRESENT 2418 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 666 ANIMAL NUMBER: C92724 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 975.4 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 11:12 PROSECTOR: CHANESTA ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY SKIN (SK) : -RAISED FOCUS(I)/AREA(S); MID DORSAL THORACIC REGION: TWO YELLOW, DARK RED AND DRY AREAS, UP TO 9 X 3 X 2 MM (CAP 23) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -ANIMAL OBESE CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2419 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 667 ANIMAL NUMBER: C92724 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 975.4 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 11:12 PROSECTOR: CHANESTA ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAr /i LIAC (LM), CAVITY, ABDOM (PC), AUDITORYSEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: TESTIS (TE), LUNG (LU), PARATHYROID (PT), URINARY BLADDER (UB) 2420 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 668 ANIMAL NUMBER: C92725 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 04/17/99 STUDY DAY OF DEATH: 363 STUDY WEEK OF DEATH: 52 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 04/17/99 9:08 PROSECTOR: STEVE VAN ADESTINE RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >RESPIRATION; AUDIBLE; VERIFIED AT NECROPSY: NOT APPLICABLE >RESPIRATION; LABORED; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; SENSITIVE TO TOUCH; VERIFIED AT NECROPSY: NOT APPLICABLE >EYES; RED DISCHARGE; EYES; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; RED HAIR COAT; HEAD MAXILLARY-LEFT; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; RED HAIR COAT; HEADMAXILLARY-RIGHT; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LN, MESENTERIC (MS) : -LARGE; UP TO 35 X 22 X 18 MM -DIFFUSELY RED; DARK RED HISTOPATHOLOGY BRAIN (BR) : -HEMORRHAGE,-MINIMAL LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MINIMAL ADRENAL, MEDULLA (MA) : -HYPERPLASIA, MEDULLARY,-MINIMAL LN, MESENTERIC (MS) : -M-HEMANGIOSARCOMA,-NEOPLASM PRESENT THYMUS (TH) : -HEMORRHAGE,-PRESENT -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATE LUNG (LU) : -CONGESTION,-PRESENT 2421 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 669 ANIMAL NUMBER: C92725 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 04/17/99 STUDY DAY OF DEATH: 363 STUDY WEEK OF DEATH: 52 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 04/17/99 9:08 PROSECTOR: STEVE VAN ADESTINE RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY STOMACH, GL (ST) : -RED FOCUS(I)/AREA(S); PYLORIC MUCOSA: SINGLE AREA, 2 X 1 MM URINARY BLADDER (UB) : -DIFFUSELY RED; DARK RED PROSTATE (PR) : -NODULAR; DIFFUSE: GREEN AND FIRM GENERAL COMMENT (GC) : -DISCHARGE-OCULAR; BILATERAL: DARK RED, DRY, CRUSTED MATERIAL -DISCHARGE-NASAL; DARK RED, DRY, CRUSTED MATERIAL; CONSISTENT WITH RED HAIR COAT (BILATERAL, MAXILLARY) AS NOTED IN CLINICAL OBSERVATIONS LUNG (LU) : -HEMORRHAGE,-PRESENT HEART (HT) : -CARDIOMYOPATHY,-SLIGHT ESOPHAGUS (ES) : -EXUDATE, LUMINAL,-PRESENT HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL STOMACH, GL (ST) : -EDEMA,-MODERATE -INFLAMMATION, ACUTE,-MINIMAL -HEMORRHAGE,-MINIMAL URINARY BLADDER (UB) : -HEMORRHAGE,-SLIGHT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT EPIDIDYMIDES (EP) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL CAUSE OF DEATH (CD) : -NEOPLASM, ABDOMINAL CAVITY,-PRESENT 2422 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 670 ANIMAL NUMBER: C92725 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 04/17/99 STUDY DAY OF DEATH: 363 STUDY WEEK OF DEATH: 52 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 04/17/99 9:08 PROSECTOR: STEVE VAN ADESTINE RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, CORTEX (AC), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), SEMINAL VESICLES HEMATO NEOPLASIA (HN) (SV), 2423 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 671 ANIMAL NUMBER: C92726 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 736.5 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 8:38 PROSECTOR: TROY ELLENBECKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY THYROID (TY) : -LARGE; RIGHT: 10 X 6 X 4 MM (LEFT IS 7 X 3 X 2 MM) PITUITARY (PI) : -RED FOCUS(I)/AREA(S); MID ASPECT: SINGLE DARK RED AREA, 4 X 3 MM HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -NECROSIS, INDIVIDUAL HEPATOCYTE,MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MODERATE -DEGENERATION, CYSTIC,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL PANCREAS (PA) : -INFILTRATION, FAT, INTERSTITIAL,MODERATE LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL THYROID (TY) : -M-CARCINOMA, FOLLICULAR CELL,-NEOPLASM PRESENT -B-ADENOMA, FOLLICULAR CELL,-NEOPLASM PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2424 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 672 ANIMAL NUMBER: C92726 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 736.5 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 8:38 PROSECTOR: TROY ELLENBECKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN GENERAL INFORMATION (XX) : >NOTE:>ANIMAL IDENTIFICATION TRANSPONDER DAMAGED AT NECROPSY THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAr /i LIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: TESTIS (TE), PARATHYROID (PT), URINARY BLADDER (UB) 2425 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 673 ANIMAL NUMBER: C92727 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 01/09/00 STUDY DAY OF DEATH: 630 STUDY WEEK OF DEATH: 90 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 01/09/00 10:04 PROSECTOR: DONIA MEYER RECORDER: DONIA MEYER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; SORE/SCAB; PAW-HINDLEFT; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -MOTTLED; ALL LOBES: DARK RED AND TAN KIDNEY (KD) : -GRANULAR MATERIAL; LEFT, WITHIN PELVIS: MULTIPLE TAN GRANULES, UP TO 2 MM IN DIAMETER -ROUGH SURFACE, DIFFUSE; BILATERAL TESTIS (TE) : -SMALL; RIGHT: 18 X 13 X 2 MM (LEFT IS 18 X 13 X 8 MM); RIGHT IS SOFT HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-MODERATELY SEVERE -HYPERPLASIA, BILE DUCT,-MINIMAL -FIBROSIS, PERIBILIARY,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SLIGHT -MINERALIZATION, PELVIC,-MODERATELY SEVERE -HYPERPLASIA, TRANSITIONAL CELL,-SLIGHT -INFLAMMATION, CHRONIC-ACTIVE, PELVIC,MODERATE PANCREAS (PA) : -ATROPHY, ACINAR,-MODERATELY SEVERE -INFILTRATION, FAT, INTERSTITIAL,MODERATELY SEVERE ADRENAL, CORTEX (AC) : -PIGMENT,-SLIGHT TESTIS (TE) : -ATROPHY/DEGENERATION,-SEVERE >NOTE:>UNILATERAL 2426 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 674 ANIMAL NUMBER: C92727 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 01/09/00 STUDY DAY OF DEATH: 630 STUDY WEEK OF DEATH: 90 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 01/09/00 10:04 PROSECTOR: DONIA MEYER RECORDER: DONIA MEYER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 10 X 9 X 5 MM, TAN AND SOFT STOMACH, GL (ST) : -RED FOCUS(I)/AREA(S); MUCOSA: MULTIPLE DARK RED AREAS, UP TO 2 X 1 MM MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,-MODERATE MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,-SEVERE THYMUS (TH) : >SECTION EXAMINED; TISSUE NOT PRESENT LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FIBROSIS,-MINIMAL -MINERALIZATION, ALVEOLAR LUMINA,MINIMAL HEART (HT) : -CARDIOMYOPATHY,-MODERATE PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : >UNREMARKABLE JEJUNUM (JE) : >AUTOLYTIC CECUM (CE) : >AUTOLYTIC URINARY BLADDER (UB) : -HYPERPLASIA, TRANSITIONAL CELL,-SLIGHT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SEVERE 2427 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 675 ANIMAL NUMBER: C92727 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 01/09/00 STUDY DAY OF DEATH: 630 STUDY WEEK OF DEATH: 90 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 01/09/00 10:04 PROSECTOR: DONIA MEYER RECORDER: DONIA MEYER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY SEMINAL VESICLES (SV) : -SMALL; BILATERAL: EACH IS 15 X 8 X 1 MM FOOT/FOOTPAD (FP) : -CRUSTED AREA(S); LEFT HIND FOOT: SINGLE DRY AREA, 5 MM IN DIAMETER; COLLECTED GENERAL COMMENT (GC) : -GI TRACT-LARGE LUMEN; ENTIRE GI TRACT: UP TO 10 MM IN DIAMETER SEMINAL VESICLES (SV) : -ATROPHY,-SEVERE CAUSE OF DEATH (CD) : -INFLAMMATION / INFECTION,-PRESENT FOOT/FOOTPAD (FP) : -INFLAMMATION, CHRONIC-ACTIVE,MODERATELY SEVERE 2428 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 676 ANIMAL NUMBER: C92727 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 01/09/00 STUDY DAY OF DEATH: 630 STUDY WEEK OF DEATH: 90 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 01/09/00 10:04 PROSECTOR: DONIA MEYER RECORDER: DONIA MEYER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAr /i LIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), ILEUM (IL), COLON (CO), RECTUM (RE), SKIN (SK), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2429 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 677 ANIMAL NUMBER: C92728 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 580.9 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 11:39 PROSECTOR: TROY ELLENBECKER RECORDER: KIMBERLY DURLAND POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) LF ADRENAL (AD0) RT ADRENAL (AD1) BRAIN (BR) LF KIDNEY (KD0) RT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LF TESTIS (TE0) RT TESTIS (TE1) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) .0439 .0398 2.1202 2.3132 2.3640 23.2456 2.0336 .8902 1.5272 1.5527 .0132 .0133 .0076 % .0069 % .3650 % .3982 % .4070 % 4.0017 % .3501 % .1532 % .2629 % .2673 % .0023 % .0023 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0207 .0188 1.0000 1.0910 1.1150 10.9639 .9592 .4199 .7203 .7323 .0062 .0063 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL: DARK RED (CAP 21) LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-MINIMAL -VACUOLATION, HEPATOCELLULAR, MIDZONAL / CENTRILOBULAR,-SLIGHT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MANDIBULAR (MN) : >NOT REQUIRED TO BE EXAMINED FOR ANIMAL 2430 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 678 ANIMAL NUMBER: C92728 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 580.9 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 11:39 PROSECTOR: TROY ELLENBECKER RECORDER: KIMBERLY DURLAND POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE) 2431 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 679 ANIMAL NUMBER: C92729 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/20/99 STUDY DAY OF DEATH: 549 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: 532.7 GRAMS DATE AND TIME OF NECROPSY: 10/20/99 13:55 PROSECTOR: JARED FISHEL RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; RED HAIR COAT; HEADENTIRE; VERIFIED AT NECROPSY: NOT OBSERVED >MASS(ES); INGUINAL REGION-LEFT; WEEK 77; 20 X 20MM; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; NO FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >EYES; RED DISCHARGE; EYES; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: NOT OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) HISTOPATHOLOGY BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SLIGHT -HYALINE DROPLETS, TUBULAR EPITHELIUM,SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,SLIGHT -ATROPHY, ACINAR,-SLIGHT ADRENAL, CORTEX (AC) : -HYPERTROPHY, CORTICAL CELL,-MINIMAL TESTIS (TE) : >UNREMARKABLE 2432 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 680 ANIMAL NUMBER: C92729 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/20/99 STUDY DAY OF DEATH: 549 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: 532.7 GRAMS DATE AND TIME OF NECROPSY: 10/20/99 13:55 PROSECTOR: JARED FISHEL RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY TESTIS (TE) : -SMALL; BILATERAL: EACH IS 18 X 10 X 6 MM; EACH IS SOFT PITUITARY (PI) : -MASS(ES); 9 MM IN DIAMETER, DARK RED AND SOFT PROSTATE (PR) : -SMALL; 15 X 10 X 5 MM SEMINAL VESICLES (SV) : -SMALL; BILATERAL: EACH IS 10 X 5 X 2 MM LN, LUMBAR/ILIAC (LM) : -CYST(S); RIGHT, INVOLVING ENTIRE NODE: SINGLE CLEAR FLUID FILLED CYST, 12 X 7 X 6 MM (CAP 22) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: RED AND DRY MATERIAL -DISCHARGE-NASAL; RED AND DRY MATERIAL HEART (HT) : -CARDIOMYOPATHY,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE SEMINAL VESICLES (SV) : -SECRETION, DECREASED,-PRESENT EPIDIDYMIDES (EP) : -HYPOSPERMIA,-PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT LN, LUMBAR/ILIAC (LM) : -LYMPHANGIECTASIS,-PRESENT 2433 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 681 ANIMAL NUMBER: C92729 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/20/99 STUDY DAY OF DEATH: 549 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: 532.7 GRAMS DATE AND TIME OF NECROPSY: 10/20/99 13:55 PROSECTOR: JARED FISHEL RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), HEMATO NEOPLASIA (HN) 2434 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 682 ANIMAL NUMBER: C92730 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 547.7 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 10:21 PROSECTOR: JARED FISHEL RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); HEAD-MAXILLARY-LEFT; WEEK 105; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; ATAXIC; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE PINPOINT DARK RED AREAS; COLLECTED ON ROUTINE SECTIONS PITUITARY (PI) : -MASS(ES); 9 MM IN DIAMETER, RED, TAN AND SOFT HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -DEGENERATION, CYSTIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SLIGHT PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-MODERATE -M-CARCINOMA, ISLET CELL,-NEOPLASM PRESENT -B-ADENOMA, ISLET CELL,- 2 NEOPLASMS PRESENT LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT SKIN (SK) : -ACANTHOSIS/HYPERKERATOSIS,-SLIGHT -INFLAMMATION, CHRONIC-ACTIVE,-SEVERE 2435 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 683 ANIMAL NUMBER: C92730 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 547.7 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 10:21 PROSECTOR: JARED FISHEL RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY SKIN (SK) : -MASS(ES); LEFT MAXILLARY REGION NEAR COMMISSURE: SINGLE MASS, 6 X 4 X 2 MM GRAY AND FIRM (CAP 22); REGIONAL LN (MANDIBULAR) NORMAL (CAP 23) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -DISCHARGE-OCULAR; BILATERAL: MATERIAL DRY RED SKIN (SK) : -ULCERATION,-MODERATE -FIBROSIS,-MODERATE CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MANDIBULAR (MN) : -HYPERPLASIA, LYMPHOPLASMACYTIC,MODERATELY SEVERE 2436 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 684 ANIMAL NUMBER: C92730 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 547.7 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 10:21 PROSECTOR: JARED FISHEL RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: TESTIS (TE), THYROID (TY), PARATHYROID (PT), URINARY BLADDER (UB) 2437 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 685 ANIMAL NUMBER: C92731 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 321.0 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 12:13 PROSECTOR: LYNN HORNER RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN STATUS LF ADRENAL (AD0) RT ADRENAL (AD1) BRAIN (BR) LF KIDNEY (KD0) RT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LF TESTIS (TE0) RT TESTIS (TE1) LF THYROID/PARA RT THYROID/PARA (TT0) (TT1) ------------------------------------ 11.3081 ------------------------------------------- ------------------------------------ 3.5232 % ------------------------------------------- ------------------------------------------------------------------------------------- NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN WEIGHT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS 2438 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 686 ANIMAL NUMBER: C92731 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 321.0 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 12:13 PROSECTOR: LYNN HORNER RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) 2439 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 687 ANIMAL NUMBER: C92732 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/18/99 STUDY DAY OF DEATH: 578 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/18/99 13:28 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY KIDNEY (KD) : -LARGE PELVIS(ES); LEFT HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-SLIGHT -FIBROSIS, PERIBILIARY,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MODERATE SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT ADRENAL, CORTEX (AC) : -VACUOLATION, DIFFUSE,-SLIGHT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATE HEART (HT) : -CARDIOMYOPATHY,-SLIGHT CAUSE OF DEATH (CD) : -UNDETERMINED MICROSCOPICALLY,-PRESENT 2440 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 688 ANIMAL NUMBER: C92732 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/18/99 STUDY DAY OF DEATH: 578 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/18/99 13:28 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAr /i LIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG), GENERAL COMMENT (GC) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2441 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 689 ANIMAL NUMBER: C92733 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/27/99 STUDY DAY OF DEATH: 617 STUDY WEEK OF DEATH: 89 TERMINAL BODY WEIGHT: 411.6 GRAMS DATE AND TIME OF NECROPSY: 12/27/99 14:38 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; NO FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; ROUGH HAIR COAT; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; RED HAIR COAT; LATERALABDOMINAL-RIGHT; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) HISTOPATHOLOGY BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -DEGENERATION, CYSTIC,-MINIMAL -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, PELVIC,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,SLIGHT -HYPERTROPHY / BASOPHILIA, ACINAR CELL,MINIMAL ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-MODERATE 2442 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 690 ANIMAL NUMBER: C92733 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/27/99 STUDY DAY OF DEATH: 617 STUDY WEEK OF DEATH: 89 TERMINAL BODY WEIGHT: 411.6 GRAMS DATE AND TIME OF NECROPSY: 12/27/99 14:38 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 10 X 9 X 9 MM, RED, TAN AND SOFT STOMACH, NONGL (SU) : -THICKENED WALL; MULTIPLE THICKENED AREAS GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN -STAINS - SKIN/HAIRCOAT; RIGHT LATERAL HEAD AND RIGHT LATERAL THORACIC REGIONS: DARK RED AND DRY ADRENAL, CORTEX (AC) : -PIGMENT,-MINIMAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),SEVERE PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL STOMACH, NONGL (SU) : >UNREMARKABLE PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 2443 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 691 ANIMAL NUMBER: C92733 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/27/99 STUDY DAY OF DEATH: 617 STUDY WEEK OF DEATH: 89 TERMINAL BODY WEIGHT: 411.6 GRAMS DATE AND TIME OF NECROPSY: 12/27/99 14:38 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUm /s KULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) (SK), 2444 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 692 ANIMAL NUMBER: C92734 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 318.2 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 12:08 PROSECTOR: LYNN HORNER RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN STATUS LF ADRENAL (AD0) RT ADRENAL (AD1) BRAIN (BR) LF KIDNEY (KD0) RT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LF TESTIS (TE0) RT TESTIS (TE1) LF THYROID/PARA RT THYROID/PARA (TT0) (TT1) ------------------------------------ 9.9230 ------------------------------------------- ------------------------------------ 3.1183 % ------------------------------------------- ------------------------------------------------------------------------------------- NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN WEIGHT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS 2445 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 693 ANIMAL NUMBER: C92734 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 318.2 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 12:08 PROSECTOR: LYNN HORNER RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) 2446 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 694 ANIMAL NUMBER: C92735 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 804.1 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 10:27 PROSECTOR: JERALD W. JOHNSON RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE DARK RED AREAS, UP TO 4 MM IN DIAMETER; COLLECTED ON ROUTINE SECTIONS THYROID (TY) : -LARGE; BILATERAL: EACH IS 7 X 5 X 3 MM GENERAL COMMENT (GC) : -BLOOD FILM TAKEN HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MODERATELY SEVERE -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-SLIGHT -HYPERPLASIA, BILE DUCT,-SLIGHT -FIBROSIS, PERIBILIARY,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SLIGHT -MINERALIZATION, PELVIC,-SLIGHT -HYPERPLASIA, TRANSITIONAL CELL,-SLIGHT PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL -INFILTRATION, FAT, INTERSTITIAL,MODERATE THYROID (TY) : -DILATATION / CYST, FOLLICULAR,-PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2447 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 695 ANIMAL NUMBER: C92735 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 804.1 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 10:27 PROSECTOR: JERALD W. JOHNSON RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN -ANIMAL OBESE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: TESTIS (TE), LUNG (LU), PARATHYROID (PT), URINARY BLADDER (UB) 2448 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 696 ANIMAL NUMBER: C92736 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 867.1 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 7:48 PROSECTOR: TODD ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY ADRENAL, CORTEX (AC) -LARGE; RIGHT: 6 MM IN DIAMETER (LEFT IS 5 X 4 X 4 MM) HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-SLIGHT -HYPERPLASIA, BILE DUCT,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MODERATE -MINERALIZATION, PELVIC,-MINIMAL -HYPERPLASIA, TRANSITIONAL CELL,-SLIGHT PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL -M-CARCINOMA, ISLET CELL,-NEOPLASM PRESENT ADRENAL, CORTEX (AC) : -PIGMENT,-SLIGHT 2449 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 697 ANIMAL NUMBER: C92736 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 867.1 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 7:48 PROSECTOR: TODD ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY TESTIS (TE) : -SMALL; RIGHT: 19 X 11 X 6 MM (LEFT IS 26 X 15 X 11 MM); RIGHT APPEARS SOFT PITUITARY (PI) : -RED FOCUS(I)/AREA(S); MID ANTERIOR ASPECT: SINGLE FOCUS, 1 MM IN DIAMETER STOMACH, GL (ST) : -THICKENED WALL; MUCOSA: DIFFUSE LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL: DARK RED; COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN GENERAL INFORMATION (XX) : >NOTE:>ANIMAL IDENTIFICATION TRANSPONDER DAMAGED AT NECROPSY ADRENAL, MEDULLA (MA) : -B-PHEOCHROMOCYTOMA,- 2 NEOPLASMS PRESENT TESTIS (TE) : -ATROPHY/DEGENERATION,-SEVERE -INFLAMMATION, VASCULAR,-SLIGHT >NOTE:>UNILATERAL LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL THYROID (TY) : -DILATATION / CYST, FOLLICULAR,-PRESENT PITUITARY (PI) : >NOT REQUIRED TO BE EXAMINED FOR ANIMAL STOMACH, GL (ST) : >UNREMARKABLE CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MANDIBULAR (MN) : -HEMORRHAGE,-PRESENT 2450 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 698 ANIMAL NUMBER: C92736 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 867.1 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 7:48 PROSECTOR: TODD ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PARATHYROID (PT), URINARY BLADDER (UB) 2451 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 699 ANIMAL NUMBER: C92737 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/16/00 STUDY DAY OF DEATH: 637 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: 566.2 GRAMS DATE AND TIME OF NECROPSY: 01/16/00 8:06 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); DORSAL; WEEK 78; 10 X 10MM; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; NO FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; CONVULSIONS; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >RESPIRATION; IRREGULAR; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-MINIMAL -DEGENERATION, CYSTIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MINIMAL PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-SEVERE -INFILTRATE, MACROPHAGE, PIGMENTED,SLIGHT ADRENAL, CORTEX (AC) : -PIGMENT,-SLIGHT MARROW, FEMUR (FM) : -HYPOCELLULAR, MARROW,-MODERATELY SEVERE 2452 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 700 ANIMAL NUMBER: C92737 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/16/00 STUDY DAY OF DEATH: 637 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: 566.2 GRAMS DATE AND TIME OF NECROPSY: 01/16/00 8:06 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 13 X 9 X 9 MM, DARK RED, TAN, AND SOFT STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA: MULTIPLE TAN ERODED AREAS, UP TO 2 MM IN DIAMETER -THICKENED WALL; DIFFUSE SKIN (SK) : -RAISED FOCUS(I)/AREA(S); MID DORSAL LUMBAR REGION: SINGLE TAN AND DRY AREA, 7 X 6 X 5 MM (CAP 22); POSSIBLY CONSISTENT WITH MASS NOTED IN CLINICAL OBSERVATIONS THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATELY SEVERE LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL HEART (HT) : -CARDIOMYOPATHY,-SLIGHT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : -HYPERPLASIA, EPITHELIAL, GLANDULAR,MODERATE STOMACH, NONGL (SU) : -ACANTHOSIS / HYPERKERATOSIS,-MODERATE -ULCERATION,-MINIMAL -INFLAMMATION, CHRONIC-ACTIVE,MODERATELY SEVERE SKIN (SK) : -B-KERATOACANTHOMA,-NEOPLASM PRESENT URINARY BLADDER (UB) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL 2453 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 701 ANIMAL NUMBER: C92737 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/16/00 STUDY DAY OF DEATH: 637 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: 566.2 GRAMS DATE AND TIME OF NECROPSY: 01/16/00 8:06 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; DARK RED AND DRY URINARY BLADDER (UB) : -PROTEINACEOUS MATERIAL, LUMINAL,PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 2454 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 702 ANIMAL NUMBER: C92737 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/16/00 STUDY DAY OF DEATH: 637 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: 566.2 GRAMS DATE AND TIME OF NECROPSY: 01/16/00 8:06 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), KIDNEY (KD), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2455 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 703 ANIMAL NUMBER: C92738 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 01/20/00 STUDY DAY OF DEATH: 641 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 01/20/00 7:58 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) ADRENAL, CORTEX (AC) : -LARGE; RIGHT: 10 X 8 X 6 MM (LEFT IS 5 X 4 X 3 MM) HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-MINIMAL -NECROSIS, COAGULATIVE,-MINIMAL -HYPERPLASIA, BILE DUCT,-SLIGHT -HYPERTROPHY, HEPATOCELLULAR, PERIPORTAL,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -DILATATION, PELVIC,-MINIMAL PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-SEVERE ADRENAL, CORTEX (AC) : -B-ADENOMA,-NEOPLASM PRESENT ADRENAL, MEDULLA (MA) : -B-PHEOCHROMOCYTOMA,-NEOPLASM PRESENT TESTIS (TE) : -M-MALIGNANT INTERSTITIAL CELL TUMOR,NEOPLASM PRESENT 2456 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 704 ANIMAL NUMBER: C92738 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 01/20/00 STUDY DAY OF DEATH: 641 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 01/20/00 7:58 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 10 X 6 X 6 MM, DARK RED, GREY, TAN AND FIRM GENERAL COMMENT (GC) : -DISCHARGE-OCULAR; BILATERAL: DRY RED MATERIAL -STAINS-PERINEUM/PERIANAL; DRY BROWN MATERIAL HEART (HT) : -CARDIOMYOPATHY,-SLIGHT THYROID (TY) : -HYPERTROPHY, FOLLICULAR CELL,-MODERATE PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT JEJUNUM (JE) : >AUTOLYTIC URINARY BLADDER (UB) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,MODERATELY SEVERE SEMINAL VESICLES (SV) : -ATROPHY,-MODERATELY SEVERE -INFILTRATE, LYMPHOHISTIOCYTIC,MODERATELY SEVERE CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 2457 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 705 ANIMAL NUMBER: C92738 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 01/20/00 STUDY DAY OF DEATH: 641 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 01/20/00 7:58 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUm /s KULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2458 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 706 ANIMAL NUMBER: C92739 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 736.7 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 10:50 PROSECTOR: STEVE VAN ADESTINE RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) -MOTTLED; ALL LOBES: RED AND TAN HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-SLIGHT -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL -DEGENERATION, CYSTIC,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -HYPERPLASIA, TRANSITIONAL CELL,-SLIGHT -INFLAMMATION, SUPPURATIVE, PELVIC,MINIMAL -THROMBUS,-PRESENT PANCREAS (PA) : -M-CARCINOMA, ISLET CELL,-NEOPLASM PRESENT THYROID (TY) : -M-CARCINOMA, FOLLICULAR CELL,-NEOPLASM PRESENT -B-ADENOMA, C-CELL,-NEOPLASM PRESENT 2459 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 707 ANIMAL NUMBER: C92739 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 736.7 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 10:50 PROSECTOR: STEVE VAN ADESTINE RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PROSTATE (PR) : -MASS(ES); INVOLVING ENTIRE PROSTATE: SINGLE MASS, 25 X 20 X 14 MM, GREEN, TAN, FIRM, LOBULATED, AND GELATINOUS; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (SEE LUMBAR) SEMINAL VESICLES (SV) : -LARGE; BILATERAL: EACH IS 25 X 10 X 10 MM; BOTH ARE FIRM -MOTTLED; BILATERAL: TAN AND GREEN LN, LUMBAR/ILIAC (LM) : -LARGE; BILATERAL: LEFT IS 2 X 6 X 3 MM, RIGHT IS 10 X 6 X 3 MM (CAP 22) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN URINARY BLADDER (UB) : -INFILTRATE, LYMPHOHISTIOCYTIC,MODERATE -PROTEINACEOUS MATERIAL, LUMINAL,PRESENT -HYPERPLASIA, TRANSITIONAL CELL,MODERATE PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SEVERE SEMINAL VESICLES (SV) : -INFLAMMATION, CHRONIC-ACTIVE,-SEVERE CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, LUMBAR/ILIAC (LM) : -LYMPHANGIECTASIS,-PRESENT -HYPERPLASIA, LYMPHOPLASMACYTIC,-SEVERE 2460 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 708 ANIMAL NUMBER: C92739 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 736.7 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 10:50 PROSECTOR: STEVE VAN ADESTINE RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOt /f OOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: TESTIS (TE), LUNG (LU), PARATHYROID (PT) 2461 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 709 ANIMAL NUMBER: C92740 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 855.2 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 8:37 PROSECTOR: TODD ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY TESTIS (TE) : -SMALL; RIGHT: 20 X 13 X 9 MM (LEFT IS 26 X 16 X 11 MM); RIGHT APPEARS SOFT STOMACH, GL (ST) : -THICKENED WALL; MUCOSA: DIFFUSE HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-MODERATE -VACUOLATION, HEPATOCELLULAR, MIDZONAL / CENTRILOBULAR,-MODERATE -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-SLIGHT PANCREAS (PA) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -INFLAMMATION, VASCULAR,-SLIGHT TESTIS (TE) : -ATROPHY/DEGENERATION,-SEVERE >NOTE:>UNILATERAL STOMACH, GL (ST) : >UNREMARKABLE CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, LUMBAR/ILIAC (LM) : -LYMPHANGIECTASIS,-PRESENT 2462 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 710 ANIMAL NUMBER: C92740 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 855.2 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 8:37 PROSECTOR: TODD ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY LN, LUMBAR/ILIAC (LM) : -LARGE; RIGHT: 11 X 9 X 5 MM (LEFT IS 6 X 3 X 2 MM); RIGHT APPEARS CYSTIC (CAP 22) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), THYROID (TY), PARATHYROID (PT), URINARY BLADDER (UB) 2463 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 711 ANIMAL NUMBER: C92741 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/12/00 STUDY DAY OF DEATH: 633 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: 449.4 GRAMS DATE AND TIME OF NECROPSY: 01/12/00 15:20 PROSECTOR: WENDY CHERRY RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; SORE/SCAB; PAWS-HIND; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; HUNCHED; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; COLD TO TOUCH; ENTIRE BODY; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; RECUMBENT; LATERAL; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) KIDNEY (KD) : -GRANULAR MATERIAL; RIGHT, WITHIN PELVIS: MULTIPLE TAN AND FIRM GRANULES, UP TO 1 MM IN DIAMETER TESTIS (TE) : -SMALL; BILATERAL: EACH IS 20 X 11 X 3 MM; BOTH ARE SOFT HISTOPATHOLOGY BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, PELVIC,-SLIGHT -HYPERPLASIA, TRANSITIONAL CELL,-SLIGHT -INFLAMMATION, SUPPURATIVE, PELVIC,SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MINIMAL ADRENAL, CORTEX (AC) : -PIGMENT,-SLIGHT -ATROPHY, CORTICAL,-MODERATE TESTIS (TE) : -ATROPHY/DEGENERATION,-SEVERE 2464 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 712 ANIMAL NUMBER: C92741 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/12/00 STUDY DAY OF DEATH: 633 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: 449.4 GRAMS DATE AND TIME OF NECROPSY: 01/12/00 15:20 PROSECTOR: WENDY CHERRY RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 15 X 10 X 10 MM, DARK RED, TAN AND SOFT STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA: MULTIPLE TAN ERODED AREAS, UP TO 3 MM IN DIAMETER URINARY BLADDER (UB) : -CALCULUS(I); WITHIN LUMEN: MULTIPLE TAN AND FIRM CALCULI, UP TO 9 MM IN DIAMETER -DIFFUSELY RED; MUCOSA: DARK RED -THICKENED WALL; DIFFUSE SEMINAL VESICLES (SV) : -SMALL; BILATERAL: EACH IS 14 X 10 X 2 MM THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),SLIGHT THYROID (TY) : -B-ADENOMA, FOLLICULAR CELL,-NEOPLASM PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, NONGL (SU) : -ACANTHOSIS / HYPERKERATOSIS,MODERATELY SEVERE -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE URINARY BLADDER (UB) : -HYPERPLASIA, TRANSITIONAL CELL,-SEVERE -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SEVERE SEMINAL VESICLES (SV) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE EPIDIDYMIDES (EP) : -VACUOLATION, TUBULAR EPITHELIUM,SLIGHT 2465 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 713 ANIMAL NUMBER: C92741 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/12/00 STUDY DAY OF DEATH: 633 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: 449.4 GRAMS DATE AND TIME OF NECROPSY: 01/12/00 15:20 PROSECTOR: WENDY CHERRY RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY LN, RENAL (RL) : -LARGE; BILATERAL: LEFT IS UP TO 20 X 10 X 10 MM, RIGHT IS UP TO 12 X 8 X 4 MM (CAP 22) TAIL (TI) : -RAISED AREA(S); ALONG ENTIRE LENGTH: MULTIPLE DARK RED AND TAN AREAS, UP TO 5 X 4 X 4 MM; COLLECTED FOOT/FOOTPAD (FP) : -CRUSTED AREA(S); BILATERAL HINDFEET: SINGLE DARK RED, TAN AND DRY AREA ON EACH, RIGHT IS 10 X 4 X 4 MM, LEFT IS 10 X 10 X 7 MM; BOTH COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN -URINARY BLADDER-LARGE LUMEN; 30 X 20 X 20 MM -STAINS - SKIN/HAIRCOAT; VENTRAL ABDOMINAL REGION: RED AND MOIST CAUSE OF DEATH (CD) : -INFECTION / NECROSIS / CALCULUS, URINARY TRACT,-PRESENT LN, RENAL (RL) : -HYPERPLASIA, LYMPHOPLASMACYTIC,-SEVERE -DILATATION, SINUSOIDAL,-SEVERE TAIL (TI) : -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL -ACANTHOSIS / HYPERKERATOSIS,-SLIGHT FOOT/FOOTPAD (FP) : -INFLAMMATION, CHRONIC-ACTIVE,-SEVERE -ULCERATION,-MODERATE -ACANTHOSIS / HYPERKERATOSIS,-MODERATE 2466 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 714 ANIMAL NUMBER: C92741 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/12/00 STUDY DAY OF DEATH: 633 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: 449.4 GRAMS DATE AND TIME OF NECROPSY: 01/12/00 15:20 PROSECTOR: WENDY CHERRY RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), PROSTATE (PR), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), HEMATO NEOPLASIA (HN) (SG), 2467 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 715 ANIMAL NUMBER: C92742 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 896.7 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 9:41 PROSECTOR: CHANESTA ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >WART LIKE LESION(S); DORSAL; WEEK 66; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; PAW-HINDRIGHT; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE DARK RED AREAS, UP TO 4 MM IN DIAMETER; COLLECTED ON ROUTINE SECTIONS PITUITARY (PI) : -MASS(ES); 8 X 4 X 2 MM, DARK RED, TAN, AND SOFT HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL -DEGENERATION, CYSTIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-SLIGHT -DEGENERATION / NECROSIS, CENTRILOBULAR,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MODERATE -ECTASIA, TUBULAR,-MODERATELY SEVERE PANCREAS (PA) : -INFILTRATION, FAT, INTERSTITIAL,SLIGHT THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PARATHYROID (PT) : -HYPERPLASIA,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT 2468 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 716 ANIMAL NUMBER: C92742 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 896.7 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 9:41 PROSECTOR: CHANESTA ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY SKIN (SK) : -CRUSTED AREA(S); MID DORSAL CERVICAL REGION: SINGLE, YELLOW, DARK RED, DRY AREA, 23 X 20 MM; COLLECTED FOOT/FOOTPAD (FP) : -CRUSTED AREA(S); RIGHT HINDFOOT: SINGLE RED, TAN, AND DRY AREA, 6 X 4 MM; COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN SKIN (SK) : -B-KERATOACANTHOMA,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT FOOT/FOOTPAD (FP) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE -ACANTHOSIS / HYPERKERATOSIS,-MODERATE 2469 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 717 ANIMAL NUMBER: C92742 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 896.7 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 9:41 PROSECTOR: CHANESTA ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: TESTIS (TE), LUNG (LU), URINARY BLADDER (UB) 2470 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 718 ANIMAL NUMBER: C92743 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/27/99 STUDY DAY OF DEATH: 587 STUDY WEEK OF DEATH: 84 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/27/99 14:06 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; OBES; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 7 MM IN DIAMETER, DARK RED, RED, TAN AND SOFT HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -NECROSIS, COAGULATIVE,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MINIMAL PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL ADRENAL, CORTEX (AC) : -PIGMENT,-SLIGHT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),SEVERE PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT DUODENUM (DU) : >AUTOLYTIC 2471 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 719 ANIMAL NUMBER: C92743 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/27/99 STUDY DAY OF DEATH: 587 STUDY WEEK OF DEATH: 84 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/27/99 14:06 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -ANIMAL OBESE JEJUNUM (JE) : >AUTOLYTIC ILEUM (IL) : >AUTOLYTIC CECUM (CE) : >AUTOLYTIC URINARY BLADDER (UB) : >AUTOLYTIC PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 2472 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 720 ANIMAL NUMBER: C92743 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/27/99 STUDY DAY OF DEATH: 587 STUDY WEEK OF DEATH: 84 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/27/99 14:06 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), COLON (CO), RECTUM (RE), SKIN (SK), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2473 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 721 ANIMAL NUMBER: C92744 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 302.1 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 12:11 PROSECTOR: JODI THIEREN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN STATUS LF ADRENAL (AD0) RT ADRENAL (AD1) BRAIN (BR) LF KIDNEY (KD0) RT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LF TESTIS (TE0) RT TESTIS (TE1) LF THYROID/PARA RT THYROID/PARA (TT0) (TT1) ------------------------------------ 11.7687 ------------------------------------------- ------------------------------------ 3.8962 % ------------------------------------------- ------------------------------------------------------------------------------------- NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN WEIGHT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS 2474 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 722 ANIMAL NUMBER: C92744 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 302.1 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 12:11 PROSECTOR: JODI THIEREN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) 2475 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 723 ANIMAL NUMBER: C92745 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/03/00 STUDY DAY OF DEATH: 624 STUDY WEEK OF DEATH: 90 TERMINAL BODY WEIGHT: 663.3 GRAMS DATE AND TIME OF NECROPSY: 01/03/00 14:05 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; SORE/SCAB; DORSAL; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); INGUINAL REGION-RIGHT; WEEK 84; 10 X 10MM; VERIFIED AT NECROPSY: NOT OBSERVED >SKIN & PELAGE; SORE/SCAB; PAWS-HIND; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; PALE; ENTIRE BODY; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -DIFFUSELY LIGHT; ALL LOBES: TAN KIDNEY (KD) : -ROUGH SURFACE, DIFFUSE; BILATERAL -DIFFUSELY LIGHT; BILATERAL: TAN HISTOPATHOLOGY EYE (EY) : -DYSTROPHY, CORNEAL IN EARLY STAGES, CONSISTS PRIMARILY OF FOCAL MINERALIZATION,-MODERATE BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -VACUOLATION, HEPATOCELLULAR, MIDZONAL / CENTRILOBULAR,-MODERATELY SEVERE -NECROSIS, COAGULATIVE,-MINIMAL -HEMATOPOIESIS, EXTRAMEDULLARY,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SEVERE SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,MODERATELY SEVERE -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL -INFILTRATION, FAT, INTERSTITIAL,MODERATE 2476 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 724 ANIMAL NUMBER: C92745 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/03/00 STUDY DAY OF DEATH: 624 STUDY WEEK OF DEATH: 90 TERMINAL BODY WEIGHT: 663.3 GRAMS DATE AND TIME OF NECROPSY: 01/03/00 14:05 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY TESTIS (TE) : -SMALL; RIGHT: 18 X 10 X 3 MM (LEFT IS 20 X 13 X 6 MM); BOTH ARE SOFT LUNG (LU) : -RED FOCUS(I)/AREA(S); VENTRAL RIGHT CAUDAL LOBE: SINGLE DARK RED AREA, 3 MM IN DIAMETER ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-SLIGHT -PIGMENT,-MINIMAL LN, MESENTERIC (MS) : -MINERALIZATION, VASCULAR,-MODERATE TESTIS (TE) : -ATROPHY/DEGENERATION,-SEVERE >NOTE:>UNILATERAL BONE, FEMUR (FE) : -HYPERPLASIA, BONE,-MODERATELY SEVERE MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,-SEVERE MARROW, STERNUM (SE) : >SECTION EXAMINED; TISSUE NOT PRESENT BONE, STERNUM (SB) : -HYPERPLASIA, CARTILAGINOUS,-MODERATE -HYPERPLASIA, BONE,-SEVERE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),SEVERE LUNG (LU) : -HEMORRHAGE,-PRESENT HEART (HT) : -CARDIOMYOPATHY,-SLIGHT -MINERALIZATION, VASCULAR,-MODERATE 2477 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 725 ANIMAL NUMBER: C92745 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/03/00 STUDY DAY OF DEATH: 624 STUDY WEEK OF DEATH: 90 TERMINAL BODY WEIGHT: 663.3 GRAMS DATE AND TIME OF NECROPSY: 01/03/00 14:05 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 9 X 6 X 5 MM, DARK BROWN, TAN AND SOFT STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: MULTIPLE DARK BROWN AREAS, UP TO 2 MM IN DIAMETER -THICKENED WALL; DIFFUSE SKIN (SK) : -CRUSTED AREA(S); MID DORSAL THORACIC REGION: SINGLE MOIST, RED AND BROWN CRUSTED AREA, 10 MM IN DIAMETER (CAP 22) SEMINAL VESICLES (SV) : -SMALL; BILATERAL: 18 X 5 X 2 MM -GELATINOUS; BILATERAL: TAN THYROID (TY) : -B-ADENOMA, FOLLICULAR CELL,-NEOPLASM PRESENT PARATHYROID (PT) : -HYPERPLASIA,-SEVERE PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : -EROSION,-MINIMAL -MINERALIZATION, GLANDULAR MUCOSA,MODERATE SKIN (SK) : -B-KERATOACANTHOMA,-NEOPLASM PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE SEMINAL VESICLES (SV) : -ATROPHY,-MODERATELY SEVERE CAUSE OF DEATH (CD) : -NEPHROPATHY,-PRESENT FOOT/FOOTPAD (FP) : -INFLAMMATION, CHRONIC-ACTIVE,-SEVERE -ULCERATION,-MODERATELY SEVERE 2478 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 726 ANIMAL NUMBER: C92745 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/03/00 STUDY DAY OF DEATH:: 624 STUDY WEEK OF DEATH: 90 TERMINAL BODY WEIGHT: 663.3 GRAMS DATE AND TIME OF NECROPSY: 01/03/00 14:05 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY FOOT/FOOTPAD (FP) : -ABRASION(S)/u LCERATION(S); BILATERAL: SINGLE MOIST AND BROWN ULCERATED AREA ON EACH, LEFT IS 6 MM IN DIAMETER, RIGHT IS 12 MM IN DIAMETER; BOTH ARE COLLECTED AORTA (AO) : -FIRM; AORTIC ARCH, DIFFUSE (CAP 23) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN AORTA (AO) : -MINERALIZATION,-SEVERE 2479 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 727 ANIMAL NUMBER: C92745 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/03/00 STUDY DAY OF DEATH: 624 STUDY WEEK OF DEATH: 90 TERMINAL BODY WEIGHT: 663.3 GRAMS DATE AND TIME OF NECROPSY: 01/03/00 14:05 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAr /i LIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: ADRENAL, MEDULLA (MA), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) (ES), 2480 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 728 ANIMAL NUMBER: C92746 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/22/99 STUDY DAY OF DEATH: 551 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: 749.0 GRAMS DATE AND TIME OF NECROPSY: 10/22/99 15:15 PROSECTOR: KEVIN BILLINGS RECORDER: KEVIN BILLINGS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; COLD TO TOUCH; ENTIRE BODY; VERIFIED AT NECROPSY: NOT APPLICABLE >EXCRETION; NO FECES; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LUNG (LU) : -MOTTLED; ALL LOBES: DARK RED AND TAN HISTOPATHOLOGY BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-MODERATE -DEGENERATION, CYSTIC,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT PANCREAS (PA) : -ATROPHY, ACINAR,-MODERATE ADRENAL, CORTEX (AC) : -PIGMENT,-MINIMAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATELY SEVERE LUNG (LU) : -CONGESTION,-PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT 2481 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 729 ANIMAL NUMBER: C92746 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/22/99 STUDY DAY OF DEATH: 551 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: 749.0 GRAMS DATE AND TIME OF NECROPSY: 10/22/99 15:15 PROSECTOR: KEVIN BILLINGS RECORDER: KEVIN BILLINGS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); SINGLE MASS, 8 X 6 X 6 DARK RED, TAN AND SOFT STOMACH, GL (ST) : -THICKENED WALL; DIFFUSE MM, STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA: MULTIPLE RED AND TAN ERODED AREAS, UP TO 6 X 3 MM -THICKENED WALL; DIFFUSE: APPEARS GELATINOUS GENERAL COMMENT (GC) : -BLOOD FILM TAKEN STOMACH, GL (ST) : -INFLAMMATION, ACUTE,-MINIMAL -HYPERPLASIA, EPITHELIAL, GLANDULAR,MODERATE STOMACH, NONGL (SU) : -ACANTHOSIS / HYPERKERATOSIS,MODERATELY SEVERE -ULCERATION,-MODERATE -INFLAMMATION, CHRONIC-ACTIVE,MODERATELY SEVERE PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL EPIDIDYMIDES (EP) : -HYPOSPERMIA,-PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 2482 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 730 ANIMAL NUMBER: C92746 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/22/99 STUDY DAY OF DEATH: 551 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: 749.0 GRAMS DATE AND TIME OF NECROPSY: 10/22/99 15:15 PROSECTOR: KEVIN BILLINGS RECORDER: KEVIN BILLINGS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), HEMATO NEOPLASIA (HN) 2483 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 731 ANIMAL NUMBER: C92747 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 716.4 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 12:38 PROSECTOR: TONY HANFELD RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; SWOLLEN; PAW-HIND-RIGHT; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; SWOLLEN; PAW-FRONT-RIGHT; VERIFIED AT NECROPSY: OBSERVED >EYES; RED DISCHARGE; EYE-RIGHT; VERIFIED AT NECROPSY: NOT OBSERVED OBSERVATIONS NECROPSY STOMACH, GL (ST) -THICKENED WALL; DIFFUSE LN, AXILLARY (AX) : -DIFFUSELY RED; BILATERAL: DARK RED (CAP 22) HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-MINIMAL -VACUOLATION, HEPATOCELLULAR, MIDZONAL / CENTRILOBULAR,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, PELVIC,-SLIGHT THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT STOMACH, GL (ST) : >UNREMARKABLE CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, AXILLARY (AX) : -HEMORRHAGE,-PRESENT -HYPERPLASIA, LYMPHOPLASMACYTIC,MODERATE -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL 2484 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 732 ANIMAL NUMBER: C92747 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 716.4 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 12:38 PROSECTOR: TONY HANFELD RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY LN, LUMBAR/ILIAC (LM) : -LARGE; RIGHT: 10 X 6 X 6 MM (LEFT IS 2 MM IN DIAMETER) (CAP 23) JOINT, OTHER (JT) : -LARGE; RIGHT CARPAL AND RIGHT TARSAL JOINT: RIGHT TARSAL IS 20 X 13 X 11 MM (LEFT IS 16 X 8 X 7 MM), RIGHT CARPAL IS 14 X 10 X 8 MM (LEFT IS 10 X 6 X 6 MM); BOTH COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN LN, LUMBAR/ILIAC (LM) : -LYMPHANGIECTASIS,-PRESENT -HYPERPLASIA, LYMPHOPLASMACYTIC,MODERATE JOINT, OTHER (JT) : -INFLAMMATION, CHRONIC-ACTIVE,MODERATELY SEVERE 2485 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 733 ANIMAL NUMBER: C92747 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 716.4 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 12:38 PROSECTOR: TONY HANFELD RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), TESTIS (TE), LUNG (LU), PARATHYROID (PT), URINARY BLADDER (UB) 2486 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 734 ANIMAL NUMBER: C92748 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 589.7 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 9:49 PROSECTOR: TONY HANFELD RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY KIDNEY (KD) : -ROUGH SURFACE, DIFFUSE; BILATERAL -LARGE; BILATERAL: EACH IS 30 X 23 X 12 MM -DIFFUSELY LIGHT; BILATERAL: TAN LN, MESENTERIC (MS) : -LARGE; DIFFUSE: UP TO 5 MM IN DIAMETER HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -NECROSIS, INDIVIDUAL HEPATOCYTE,MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -DEGENERATION, CYSTIC,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SEVERE PANCREAS (PA) : -INFLAMMATION, VASCULAR,-SEVERE LN, MESENTERIC (MS) : -HYPERPLASIA, LYMPHOPLASMACYTIC,MODERATE -INFLAMMATION, VASCULAR,-SEVERE TESTIS (TE) : -INFLAMMATION, VASCULAR,-MODERATE LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT 2487 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 735 ANIMAL NUMBER: C92748 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 589.7 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 9:49 PROSECTOR: TONY HANFELD RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PROSTATE (PR) : -NODULAR; PROXIMAL, SURROUNDING URETHRA: GREEN AND FIRM LN, RENAL (RL) : -LARGE; BILATERAL: EACH IS UP TO 7 X 5 X 3 MM (CAP 22) LN, LUMBAR/ILIAC (LM) : -LARGE; BILATERAL: EACH IS UP TO 11 X 8 X 5 MM (CAP 23) CAVITY, ABDOM (PC) : -LARGE VESSELS; INVOLVING ENTIRE MESENTERY: UP TO 2 MM IN DIAMETER (CAP 24) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN PARATHYROID (PT) : -HYPERPLASIA,-MODERATE PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, RENAL (RL) : -HYPERPLASIA, LYMPHOPLASMACYTIC,-SLIGHT LN, LUMBAR/ILIAC (LM) : -HYPERPLASIA, LYMPHOPLASMACYTIC,-SLIGHT CAVITY, ABDOM (PC) : -INFLAMMATION, VASCULAR,-SEVERE -THROMBUS,-PRESENT 2488 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 736 ANIMAL NUMBER: C92748 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 589.7 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 9:49 PROSECTOR: TONY HANFELD RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: URINARY BLADDER (UB) 2489 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 737 ANIMAL NUMBER: C92749 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 04/06/00 STUDY DAY OF DEATH: 718 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 04/06/00 9:59 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY TESTIS (TE) : -SMALL; RIGHT: 18 X 14 X 10 MM (LEFT IS 25 X 17 X 14 MM); RIGHT IS SOFT HISTOPATHOLOGY LIVER (LI) : -VACUOLATION, HEPATOCELLULAR, MIDZONAL / CENTRILOBULAR,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SLIGHT TESTIS (TE) : -ATROPHY/DEGENERATION,-SEVERE >NOTE:>UNILATERAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATELY SEVERE HEART (HT) : -CARDIOMYOPATHY,-MODERATE THYROID (TY) : -DILATATION / CYST, FOLLICULAR,-PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT JEJUNUM (JE) : >AUTOLYTIC 2490 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 738 ANIMAL NUMBER: C92749 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 04/06/00 STUDY DAY OF DEATH: 718 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 04/06/00 9:59 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -ANIMAL OBESE ILEUM (IL) : >AUTOLYTIC CECUM (CE) : >AUTOLYTIC URINARY BLADDER (UB) : >AUTOLYTIC PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT CAUSE OF DEATH (CD) : -UNDETERMINED MICROSCOPICALLY,-PRESENT 2491 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 739 ANIMAL NUMBER: C92749 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 04/06/00 STUDY DAY OF DEATH: 718 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 04/06/00 9:59 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), COLON (CO), RECTUM (RE), SKIN (SK), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2492 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 740 ANIMAL NUMBER: C92750 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 660.4 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 12:33 PROSECTOR: TROY ELLENBECKER RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 10 X 7 X 5 MM, RED, TAN AND SOFT STOMACH, GL (ST) : -EROSION/ULCERATION; MUCOSA, ALONG GREATER CURVATURE: SINGLE RED, ERODED AREA, 5 MM IN DIAMETER HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -DEGENERATION, CYSTIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SLIGHT PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-MODERATE -INFILTRATION, FAT, INTERSTITIAL,MODERATE LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-MODERATE PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : -EROSION,-MODERATE -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT 2493 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 741 ANIMAL NUMBER: C92750 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 660.4 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 12:33 PROSECTOR: TROY ELLENBECKER RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN URINARY BLADDER (UB) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUm /s KULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: TESTIS (TE), PARATHYROID (PT) 2494 COVAH^ THE DEVELOPMENT SERVICES COMPANY Sponsor: 3M St. Paul, Minnesota FINAL REPORT Study Title: 104-Week Dietary Chronic Toxicity and Carcinogenicity Study with Perfluorooctane Sulfonic Acid Potassium Salt (PFOS; T-6295) in Rats Author: Peter J. Thomford, PhD Study Completion Date: January 2, 2002 Testing Facility: Covance Laboratories Inc. 3301 Kinsman Boulevard Madison, Wisconsin 53704-2595 Laboratory Study Identification: Covance 6329-183 Sponsor Study Identification: 3M T-6295 Volume VII of IX Page 2495 of 4068 Covance 6329-183 3M T-6295 VOLUME VII OF IX CONTENTS Page APPENDIX 6 (Continued)........................................................................................... 2497 Individual Anatomic Pathology Data....................................................................... 2498 2496 APPENDIX 6 (Continued) Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 2497 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 742 ANIMAL NUMBER: C92751 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 801.5 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 10:39 PROSECTOR: JOHN S. HALFORD RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT' (%) LF ADRENAL (AD0) RT ADRENAL (AD1) BRAIN (BR) LF KIDNEY (KD0) RT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LF TESTIS (TE0) RT TESTIS (TE1) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) .0273 .0281 2.3191 2.4820 2.4333 32.0284 2.3737 .9244 1.5886 1.6268 .0240 .0264 .0034 % .0035 % .2893 % .3097 % .3036 % 3.9961 % .2962 % .1153 % .1982 % .2030 % .0030 % .0033 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0118 .0121 1.0000 1.0702 1.0492 13.8107 1.0235 .3986 .6850 .7015 .0103 .0114 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-MODERATE -HYPERTROPHY, HEPATOCELLULAR,MODERATELY SEVERE KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -PIGMENT, TUBULAR,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MINIMAL 2498 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 743 ANIMAL NUMBER: C92751 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 801.5 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 10:39 PROSECTOR: JOHN S. HALFORD RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY STOMACH, GL (ST) : -THICKENED WALL; MUCOSA STOMACH, NONGL (SU) : -THICKENED WALL; MUCOSA GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL ADRENAL, CORTEX (AC) : -HYPERTROPHY, CORTICAL CELL,-MINIMAL LN, MESENTERIC (MS) : -HEMORRHAGE,-PRESENT MARROW, FEMUR (FM) : -HYPOCELLULAR, MARROW,-SLIGHT THYMUS (TH) : -HEMORRHAGE,-PRESENT HEART (HT) : -CARDIOMYOPATHY,-SLIGHT HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL STOMACH, GL (ST) : >UNREMARKABLE STOMACH, NONGL (SU) : >UNREMARKABLE RECTUM (RE) : -PARASITISM,-PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2499 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 744 ANIMAL NUMBER: C92751 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 801.5 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 10:39 PROSECTOR: JOHN S. HALFORD RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAr /i LIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), ADRENAL, MEDULLA (MA), TESTIS (TE), BONE, FEMUR (FE), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM ILEUM (IL), CECUM (CE), COLON (CO), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) (JE), 2500 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 745 ANIMAL NUMBER: C92752 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 274.8 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 10:49 PROSECTOR: JODI THIEREN RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN STATUS LF ADRENAL (AD0) RT ADRENAL (AD1) BRAIN (BR) LF KIDNEY (KD0) RT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LF TESTIS (TE0) RT TESTIS (TE1) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) ------------------------------------ 11.7254 ------------------------------------------- ------------------------------------ 4.2670 % ------------------------------------------- ------------------------------------------------------------------------------------- NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN WEIGHT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS 2501 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 746 ANIMAL NUMBER: C92752 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 274.8 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 10:49 PROSECTOR: JODI THIEREN RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) 2502 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 747 ANIMAL NUMBER: C92753 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 733.7 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 10:51 PROSECTOR: TERRI L. WILLIAMS RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; MISSING; TEETH; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) KIDNEY (KD) : -LARGE PELVIS(ES); RIGHT HISTOPATHOLOGY BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -DILATATION, PELVIC,-MODERATE -THROMBUS,-PRESENT SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SLIGHT PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-MODERATE -INFILTRATION, FAT, INTERSTITIAL,SLIGHT 2503 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 748 ANIMAL NUMBER: C92753 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 733.7 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 10:51 PROSECTOR: TERRI L. WILLIAMS RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 9 X 6 X 6 MM, RED, DARK RED, GRAY, TAN, AND SOFT PROSTATE (PR) : -NODULAR; PROXIMAL SURROUNDING URETHRA: GREEN AND FIRM GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -MALOCCLUSION; BILATERAL UPPER INCISORS -TEETH MISSING/BROKEN/WORN; BILATERAL UPPER INCISORS: BROKEN AT BASE -ANIMAL OBESE ADRENAL, CORTEX (AC) : -HYPERTROPHY, CORTICAL CELL,-MINIMAL -PIGMENT,-MINIMAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATELY SEVERE HEART (HT) : -CARDIOMYOPATHY,-MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-SLIGHT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2504 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 749 ANIMAL NUMBER: C92753 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 733.7 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 10:51 PROSECTOR: TERRI L. WILLIAMS RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2505 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 750 ANIMAL NUMBER: C92754 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 553.8 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 11:23 PROSECTOR: GREGORY CODDINGTON RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT' (%) LF ADRENAL (AD0) RT ADRENAL (AD1) BRAIN (BR) LF KIDNEY (KD0) RT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LF TESTIS (TE0) RT TESTIS (TE1) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) .0265 .0263 2.4325 2.1434 2.1609 20.2188 2.1405 .7109 1.5214 1.5586 .0153 .0164 .0048 % .0047 % .4392 % .3870 % .3902 % 3.6509 % .3865 % .1284 % .2747 % .2814 % .0028 % .0030 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0109 .0108 1.0000 .8812 .8883 8.3119 .8800 .2923 .6254 .6407 .0063 .0067 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-MODERATE -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-MODERATE SPLEEN (SP) : -PIGMENT, INCREASED,-MINIMAL PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL -ATROPHY, ACINAR,-MINIMAL 2506 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 751 ANIMAL NUMBER: C92754 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 553.8 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 11:23 PROSECTOR: GREGORY CODDINGTON RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN ADRENAL, CORTEX (AC) : -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL -HYPERTROPHY, CORTICAL CELL,-SLIGHT URINARY BLADDER (UB) : -PROTEINACEOUS MATERIAL, LUMINAL,PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2507 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 752 ANIMAL NUMBER: C92754 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 553.8 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 11:23 PROSECTOR: GREGORY CODDINGTON RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2508 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 753 ANIMAL NUMBER: C92755 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 695.3 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 10:55 PROSECTOR: GREGORY CODDINGTON RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ORGAN NAME LF ADRENAL (AD0) RT ADRENAL (AD1) BRAIN (BR) LF KIDNEY (KD0) RT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LF TESTIS (TE0) RT TESTIS (TE1) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) ABSOLUTE ORGAN WEIGHT (GRAMS) .0201 .0226 2.2492 2.3890 2.4440 28.2494 1.8793 .8195 1.7651 1.8308 .0200 .0170 ORGAN WEIGHT RELATIVE TO BODY WEIGHT' (%) .0029 % .0033 % .3235 % .3436 % .3515 % 4.0629 % .2703 % .1179 % .2539 % .2633 % .0029 % .0024 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0089 .0100 1.0000 1.0622 1.0866 12.5598 .8355 .3644 .7848 .8140 .0089 .0076 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-MODERATE -VACUOLATION, HEPATOCELLULAR, MIDZONAL / CENTRILOBULAR,-MODERATE -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL KIDNEY (KD) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL 2509 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 754 ANIMAL NUMBER: C92755 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 695.3 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 10:55 PROSECTOR: GREGORY CODDINGTON RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY STOMACH, GL (ST) : -RED FOCUS(I)/AREA(S); MUCOSA: MULTIPLE DARK RED AREAS, UP TO 3 X 1 MM GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL ADRENAL, CORTEX (AC) : -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL MARROW, FEMUR (FM) : -HYPOCELLULAR, MARROW,-SLIGHT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MINIMAL LUNG (LU) : -HEMORRHAGE,-PRESENT -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL STOMACH, GL (ST) : -CONGESTION,-PRESENT EPIDIDYMIDES (EP) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2510 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 755 ANIMAL NUMBER: C92755 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 695.3 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 10:55 PROSECTOR: GREGORY CODDINGTON RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), HEMATO NEOPLASIA (HN) (SN), 2511 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 756 ANIMAL NUMBER: C92756 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/24/99 STUDY DAY OF DEATH: 614 STUDY WEEK OF DEATH: 88 TERMINAL BODY WEIGHT: 565.5 GRAMS DATE AND TIME OF NECROPSY: 12/24/99 7:38 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); DORSAL; WEEK 84; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; DORSAL; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) KIDNEY (KD) : -LARGE PELVIS(ES); BILATERAL HISTOPATHOLOGY BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED.-MODERATE -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -DILATATION, PELVIC,-SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-MODERATE -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL -INFILTRATION, FAT, INTERSTITIAL,SLIGHT 2512 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 757 ANIMAL NUMBER: C92756 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/24/99 STUDY DAY OF DEATH: 614 STUDY WEEK OF DEATH: 88 TERMINAL BODY WEIGHT: 565.5 GRAMS DATE AND TIME OF NECROPSY: 12/24/99 7:38 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 14 X 10 X 10 MM, DARK RED, GRAY, AND SOFT STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA: MULTIPLE TAN ERODED AREAS, UP TO 5 X 4 MM -THICKENED WALL; DIFFUSE SKIN (SK) : -CRUSTED AREA(S); MID DORSAL THORACIC REGION: SINGLE TAN, DRY, AND RAISED AREA, 7 X 7 X 6 MM; COLLECTED; POSSIBLY CONSISTENT WITH MASS AND SCAB NOTED IN CLINICAL OBSERVATIONS ADRENAL, CORTEX (AC) : -HYPERTROPHY, CORTICAL CELL,-MINIMAL -PIGMENT,-MINIMAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),SEVERE LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL HEART (HT) : -CARDIOMYOPATHY,-MINIMAL THYROID (TY) : -DILATATION / CYST, FOLLICULAR,-PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, NONGL (SU) : -ACANTHOSIS / HYPERKERATOSIS,-MODERATE -ULCERATION,-MODERATE -INFLAMMATION, CHRONIC-ACTIVE,MODERATELY SEVERE SKIN (SK) : -B-KERATOACANTHOMA,-NEOPLASM PRESENT 2513 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 758 ANIMAL NUMBER: C92756 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/24/99 STUDY DAY OF DEATH: 614 STUDY WEEK OF DEATH: 88 TERMINAL BODY WEIGHT: 565.5 GRAMS DATE AND TIME OF NECROPSY: 12/24/99 7:38 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN URINARY BLADDER (UB) : -PROTEINACEOUS MATERIAL, LUMINAL,PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 2514 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 759 ANIMAL NUMBER: C92756 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/24/99 STUDY DAY OF DEATH: 614 STUDY WEEK OF DEATH: 88 TERMINAL BODY WEIGHT: 565.5 GRAMS DATE AND TIME OF NECROPSY: 12/24/99 7:38 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2515 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 760 ANIMAL NUMBER: C92757 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 576.9 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 8:00 PROSECTOR: TROY ELLENBECKER RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); DORSAL; WEEK 98; 10 X 10MM; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-MODERATE -GRANULAR CYTOPLASM, EOSINOPHILIC, CENTRILOBULAR,-MODERATE -PIGMENT, HEPATOCELLULAR, CENTRILOBULAR,-SLIGHT -NECROSIS, INDIVIDUAL HEPATOCYTE,MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MODERATELY SEVERE -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-SLIGHT -DEGENERATION, CYSTIC,-MINIMAL -NECROSIS, COAGULATIVE,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL LIVER-OIL RED O (LI0) : -VACUOLES, OIL RED O-POSITIVE, HEPATOCELLULAR,-MODERATE 2516 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 761 ANIMAL NUMBER: C92757 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 576.9 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 8:00 PROSECTOR: TROY ELLENBECKER RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 12 X 8 X 6 MM, RED, DARK RED, TAN, AND SOFT SKIN (SK) : -MASS(ES); LEFT DORSAL THORACIC REGION: SINGLE MASS, 15 X 15 X 10 MM, TAN AND FIRM; SAME ON CUT SURFACE; SOME SECTIONS EXUDE A TAN CASEOUS MATERIAL; COLLECTED; REGIONAL LN (LEFT AXILLARY) NORMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SLIGHT PANCREAS (PA) : -HYPERPLASIA, ISLET CELLS,-SLIGHT -ATROPHY, ACINAR,-MINIMAL ADRENAL, CORTEX (AC) : -PIGMENT,-MINIMAL TESTIS (TE) : -ATROPHY/DEGENERATION,-SEVERE >NOTE:>UNILATERAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATELY SEVERE HEART (HT) : -CARDIOMYOPATHY,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT SKIN (SK) : -CYST, EPIDERMAL INCLUSION,-PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT 2517 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 762 ANIMAL NUMBER: C92757 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 576.9 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 8:00 PROSECTOR: TROY ELLENBECKER RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PROSTATE (PR) : -NODULAR; PROXIMAL SURROUNDING URETHRA: GREEN AND FIRM SEMINAL VESICLES (SV) : -LARGE; BILATERAL: EACH IS UP TO 35 X 20 X 6 MM GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -MALOCCLUSION; BILATERAL UPPER INCISORS SEMINAL VESICLES (SV) : -SECRETION, INCREASED,-PRESENT EPIDIDYMIDES (EP) : -HYPOSPERMIA,-PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2518 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 763 ANIMAL NUMBER: C92757 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 576.9 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 8:00 PROSECTOR: TROY ELLENBECKER RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUm /s KULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), HEMATO NEOPLASIA (HN), LN, AXILLARY (AX) (SN), 2519 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 764 ANIMAL NUMBER: C92758 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/21/99 STUDY DAY OF DEATH: 428 STUDY WEEK OF DEATH: 62 TERMINAL BODY WEIGHT: 407.5 GRAMS DATE AND TIME OF NECROPSY: 06/21/99 15:05 PROSECTOR: JAMES T. CALLEN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; ROUGH HAIR COAT; VERIFIED AT NECROPSY: NOT APPLICABLE >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-SLIGHT -GRANULAR CYTOPLASM, EOSINOPHILIC, CENTRILOBULAR,-MINIMAL -NECROSIS, INDIVIDUAL HEPATOCYTE,MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL ADRENAL, CORTEX (AC) : -PIGMENT,-MINIMAL TESTIS (TE) : -ATROPHY/DEGENERATION,-SEVERE >NOTE:>UNILATERAL 2520 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 765 ANIMAL NUMBER: C92758 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/21/99 STUDY DAY OF DEATH: 428 STUDY WEEK OF DEATH: 62 TERMINAL BODY WEIGHT: 407.5 GRAMS DATE AND TIME OF NECROPSY: 06/21/99 15:05 PROSECTOR: JAMES T. CALLEN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 12 X 9 X 8 MM, TAN, RED BROWN AND FIRM GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SEVERE CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 2521 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 766 ANIMAL NUMBER: C92758 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/21/99 STUDY DAY OF DEATH: 428 STUDY WEEK OF DEATH: 62 TERMINAL BODY WEIGHT: 407.5 GRAMS DATE AND TIME OF NECROPSY: 06/21/99 15:05 PROSECTOR: JAMES T. CALLEN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2522 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 767 ANIMAL NUMBER: C92759 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 320.3 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 8:22 PROSECTOR: JODI THIEREN RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN STATUS LF ADRENAL (AD0) RT ADRENAL (AD1) BRAIN (BR) LF KIDNEY (KD0) RT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LF TESTIS (TE0) RT TESTIS (TE1) LF THYROID/PARA RT THYROID/PARA (TT0) (TT1) ------------------------------------ 15.7536 ------------------------------------------- ------------------------------------ 4.9187 % ------------------------------------------- ------------------------------------------------------------------------------------- NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN WEIGHT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS 2523 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 768 ANIMAL NUMBER: C92759 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 320.3 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 8:22 PROSECTOR: JODI THIEREN RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) 2524 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 769 ANIMAL NUMBER: C92760 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/13/99 STUDY DAY OF DEATH: 512 STUDY WEEK OF DEATH: 74 TERMINAL BODY WEIGHT: 489.5 GRAMS DATE AND TIME OF NECROPSY: 09/13/99 11:20 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >EYES; RED DISCHARGE; EYES; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: NOT OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE DARK RED AREAS, UP TO 4 MM IN DIAMETER; COLLECTED ON ROUTINE SECTIONS KIDNEY (KD) : -MOTTLED; BILATERAL: BROWN AND DARK BROWN PITUITARY (PI) : -MASS(ES);r 10 X 8 X 7 MM, RED, DARK RED, TAN, BROWN, AND SOFT STOMACH, GL (ST) : -EROSION/ULCERATION; MUCOSA: SINGLE TAN ERODED AREA, 2 MM IN DIAMETER HISTOPATHOLOGY BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -ANGIECTASIS,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL THYMUS (TH) : >SECTION EXAMINED; TISSUE NOT PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : -INFLAMMATION, ACUTE,-MINIMAL -EROSION,-MINIMAL 2525 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 770 ANIMAL NUMBER: C92760 SEX: :MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/13/99 STUDY DAY OF DEATH: 512 STUDY WEEK OF DEATH: 74 DATE AND TIME OF NECROPSY: 09/13/99 11:20 PROSECTOR: TERRI L. WILLIAMS TERMINAL BODY WEIGHT: 489.5 GRAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: RED EPIDIDYMIDES (EP) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 2526 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 771 ANIMAL NUMBER: C92760 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/13/99 STUDY DAY OF DEATH: 512 STUDY WEEK OF DEATH: 74 TERMINAL BODY WEIGHT: 489.5 GRAMS DATE AND TIME OF NECROPSY: 09/13/99 11:20 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), HEMATO NEOPLASIA (HN) 2527 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 772 ANIMAL NUMBER: C92761 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 501.4 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 13:05 PROSECTOR: JARED FISHEL RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -DEGENERATION, CYSTIC,-SLIGHT -HYPERPLASIA, BILE DUCT,-MINIMAL -FIBROSIS, PERIBILIARY,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SLIGHT PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-MODERATE -INFILTRATION, FAT, INTERSTITIAL,SLIGHT ADRENAL, CORTEX (AC) : -HYPERPLASIA, CORTICAL,-MINIMAL THYMUS (TH) : >SECTION EXAMINED; TISSUE NOT PRESENT 2528 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 773 ANIMAL NUMBER: C92761 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 501.4 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 13:05 PROSECTOR: JARED FISHEL RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -RED FOCUS(I)/AREA(S); LEFT ANTERIOR ASPECT: SINGLE DARK RED AREA, 2 MM IN DIAMETER GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN HEART (HT) : -CARDIOMYOPATHY,-SLIGHT THYROID (TY) : -DILATATION / CYST, FOLLICULAR,-PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2529 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 774 ANIMAL NUMBER: C92761 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 501.4 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 13:05 PROSECTOR: JARED FISHEL RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAr /i LIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2530 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 775 ANIMAL NUMBER: C92762 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 645.3 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 8:20 PROSECTOR: DAVID SCHUETTE RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -MASS(ES); LEFT LATERAL LOBE: SINGLE MASS, 40 X 30 X 15 MM, DARK RED, BROWN, TAN, AND SOFT; SAME ON CUT SURFACE; COLLECTED ON ROUTINE SECTION; REGIONAL LN (HEPATIC) NORMAL (CAP 22) HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-SLIGHT -GRANULAR CYTOPLASM, EOSINOPHILIC, CENTRILOBULAR,-SLIGHT -NECROSIS, INDIVIDUAL HEPATOCYTE,MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL -FIBROSIS, PERIBILIARY,-MINIMAL -B-ADENOMA, HEPATOCELLULAR,-NEOPLASM PRESENT LIVER-OIL RED O (LI0) : -VACUOLES, OIL RED O-POSITIVE, HEPATOCELLULAR,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL 2531 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 776 ANIMAL NUMBER: C92762 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 645.3 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 8:20 PROSECTOR: DAVID SCHUETTE RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY TESTIS (TE) : -SMALL; BILATERAL: LEFT IS 17 X 13 X 4 MM, RIGHT IS 15 X 11 X 4 MM; BOTH ARE SOFT PITUITARY (PI) : -MASS(ES); 9 X 8 X 5 MM, DARK RED, TAN, AND SOFT STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: SINGLE DARK BROWN LINEAR AREA, 3 X 1 MM KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-SEVERE TESTIS (TE) : -ATROPHY/DEGENERATION,-SEVERE -INFILTRATE, LYMPHOHISTIOCYTIC,-SLIGHT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATE LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL HEART (HT) : -CARDIOMYOPATHY,-SLIGHT THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL -B-ADENOMA, FOLLICULAR CELL,-NEOPLASM PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : -HYPERPLASIA, EPITHELIAL, GLANDULAR,SLIGHT 2532 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 777 ANIMAL NUMBER: C92762 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 645.3 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 8:20 PROSECTOR: DAVID SCHUETTE RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY STOMACH, GL (ST) : -THICKENED WALL; DIFFUSE GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE EPIDIDYMIDES (EP) : -HYPOSPERMIA,-PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, OTHER (LN) : >TISSUE MISSING >NOTE:>HEPATIC 2533 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 778 ANIMAL NUMBER: C92762 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 645.3 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 8:20 PROSECTOR: DAVID SCHUETTE RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), HEMATO NEOPLASIA (HN) 2534 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 779 ANIMAL NUMBER: C92763 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/01/00 STUDY DAY OF DEATH: 653 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: 803.2 GRAMS DATE AND TIME OF NECROPSY: 02/01/00 11:48 PROSECTOR: STEVE VAN ADESTINE RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; SWOLLEN; PAWS-HIND; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; YELLOW HAIR COAT; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; BROWN HAIR COAT; PERINEAL AREA; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -MOTTLED; ALL LOBES: DARK RED AND TAN KIDNEY (KD) : -ROUGH SURFACE, DIFFUSE; BILATERAL -DIFFUSELY DARK; BILATERAL: DARK BROWN HISTOPATHOLOGY LIVER (LI) : -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-SLIGHT -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MODERATELY SEVERE SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MINIMAL PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-MODERATE -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL -ATROPHY, ACINAR,-SLIGHT ADRENAL, CORTEX (AC) : -PIGMENT,-MINIMAL ADRENAL, MEDULLA (MA) : -HYPERPLASIA, MEDULLARY,-MODERATE TESTIS (TE) : -INFLAMMATION, VASCULAR,-SLIGHT MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,MODERATELY SEVERE 2535 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 780 ANIMAL NUMBER: C92763 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/01/00 STUDY DAY OF DEATH: 653 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: 803.2 GRAMS DATE AND TIME OF NECROPSY: 02/01/00 11:48 PROSECTOR: STEVE VAN ADESTINE RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: MULTIPLE DARK BROWN AREAS, UP TO 4 X 2 MM -THICKENED WALL; DIFFUSE SEMINAL VESICLES (SV) : -SMALL; BILATERAL: EACH IS UP TO 13 X 6 X 2 MM MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,MODERATELY SEVERE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MINIMAL LUNG (LU) : -B-ADENOMA, BRONCHIOLAR-ALVEOLAR,NEOPLASM PRESENT MUSCLE, SKELETAL (SM) : -ATROPHY,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : -EROSION,-SLIGHT -HYPERPLASIA, EPITHELIAL, GLANDULAR,MODERATELY SEVERE SKIN (SK) : -ACANTHOSIS/HYPERKERATOSIS,-MODERATE -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE SEMINAL VESICLES (SV) : -SECRETION, DECREASED,-PRESENT EPIDIDYMIDES (EP) : -IMMATURE SPERM FORMS,-SLIGHT 2536 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 781 ANIMAL NUMBER: C92763 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/01/00 STUDY DAY OF DEATH: 653 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: 803.2 GRAMS DATE AND TIME OF NECROPSY: 02/01/00 11:48 PROSECTOR: STEVE VAN ADESTINE RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY EPIDIDYMIDES (EP) : -SMALL; BILATERAL: EACH IS 45 X 4 X 3 MM JOINT, OTHER (JT) : -LARGE; BILATERAL TARSAL JOINTS: EACH IS UP TO 13 MM IN DIAMETER; COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -STAINS-PERINEUM/PERIANAL; PERINEUM: BROWN, MOIST MATERIAL -STAINS - SKIN/HAIRCOAT; ENTIRE VENTRAL SURFACE: YELLOW AND MOIST EPIDIDYMIDES (EP) : -ATROPHY/DEGENERATION,-SLIGHT CAUSE OF DEATH (CD) : -INFLAMMATION / INFECTION,-PRESENT JOINT, OTHER (JT) : -INFLAMMATION, CHRONIC-ACTIVE,-SEVERE 2537 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 782 ANIMAL NUMBER: C92763 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/01/00 STUDY DAY OF DEATH: 653 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: 803.2 GRAMS DATE AND TIME OF NECROPSY: 02/01/00 11:48 PROSECTOR: STEVE VAN ADESTINE RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LN, MESENTERIC (MS), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), HEMATO NEOPLASIA (HN) 2538 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 783 ANIMAL NUMBER: C92764 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/01/99 STUDY DAY OF DEATH: 530 STUDY WEEK OF DEATH: 76 TERMINAL BODY WEIGHT: 393.7 GRAMS DATE AND TIME OF NECROPSY: 10/01/99 14:07 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; ATAXIC; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; HUNCHED; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; COLD TO TOUCH; LIMBS-ALL; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; COLD TO TOUCH; TAIL; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -LIGHT FOCUS(I)/AREA(S); DIAPHRAGMATIC SURFACE OF RIGHT MEDIAN LOBE: SINGLE TAN AREA, 2 X 1 MM; COLLECTED ON ROUTINE SECTION HISTOPATHOLOGY BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -DEGENERATION, CYSTIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL -FIBROSIS, PERIBILIARY,-SLIGHT -INFILTRATE, MACROPHAGE, PIGMENTED,SLIGHT -VACUOLATION, HEPATOCELLULAR,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-SLIGHT -HYPERTROPHY, CORTICAL CELL,-MINIMAL -PIGMENT,-SLIGHT 2539 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 784 ANIMAL NUMBER: C92764 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/01/99 STUDY DAY OF DEATH: 530 STUDY WEEK OF DEATH: 76 TERMINAL BODY WEIGHT: 393.7 GRAMS DATE AND TIME OF NECROPSY: 10/01/99 14:07 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); SINGLE MASS, 15 X 10 X 10 MM, RED, DARK RED, TAN AND FIRM STOMACH, GL (ST) : -EROSION/ULCERATION; MUCOSA, ALONG GREATER CURVATURE: SINGLE BLACK ERODED AREA, 2 X 1 MM GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),SEVERE LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : >UNREMARKABLE PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 2540 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 785 ANIMAL NUMBER: C92764 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/01/99 STUDY DAY OF DEATH: 530 STUDY WEEK OF DEATH: 76 TERMINAL BODY WEIGHT: 393.7 GRAMS DATE AND TIME OF NECROPSY: 10/01/99 14:07 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) (SN), 2541 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 786 ANIMAL NUMBER: C92765 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 493.3 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 12:52 PROSECTOR: STEVE VAN ADESTINE RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT' (%) LF ADRENAL (AD0) RT ADRENAL (AD1) BRAIN (BR) LF KIDNEY (KD0) RT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LF TESTIS (TE0) RT TESTIS (TE1) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) .0267 .0260 2.2505 1.6982 1.6461 18.5015 1.8585 .8329 1.6630 1.7142 .0113 .0118 .0054 % .0053 % .4562 % .3443 % .3337 % 3.7506 % .3767 % .1688 % .3371 % .3475 % .0023 % .0024 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0119 .0116 1.0000 .7546 .7314 8.2211 .8258 .3701 .7389 .7617 .0050 .0052 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED KIDNEY (KD) : -LARGE PELVIS(ES); LEFT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-SLIGHT KIDNEY (KD) : -DILATATION, PELVIC,-MODERATELY SEVERE -MINERALIZATION, PELVIC,-SLIGHT >NOTE:>RENAL LESIONS ARE UNILATERAL. SPLEEN (SP) : -PIGMENT, INCREASED,-MINIMAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2542 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 787 ANIMAL NUMBER: C92765 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 493.3 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 12:52 PROSECTOR: STEVE VAN ADESTINE RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAr /i LIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE) 2543 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 788 ANIMAL NUMBER: C92766 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/24/00 STUDY DAY OF DEATH: 705 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/24/00 7:57 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); RECTUM; WEEK 99; VERIFIED AT NECROPSY: NOT OBSERVED OBSERVATIONS NECROPSY HISTOPATHOLOGY LIVER (LI) : -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -HYPERPLASIA, TRANSITIONAL CELL,MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MINIMAL PANCREAS (PA) : -INFILTRATION, FAT, INTERSTITIAL,SLIGHT ADRENAL, CORTEX (AC) : -HYPERTROPHY, CORTICAL CELL,-SLIGHT -PIGMENT,-MINIMAL SPINAL CORD (SC) : -MINERALIZATION, DURA,-SLIGHT THYMUS (TH) : -HEMORRHAGE,-PRESENT 2544 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 789 ANIMAL NUMBER: C92766 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/24/00 STUDY DAY OF DEATH: 705 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/24/00 7:57 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY SKIN (SK) : -RAISED FOCUS(I)/AREA(S); OVERLYING RIGHT TESTIS: SINGLE RED, RAISED AREA, 4 MM IN DIAMETER (CAP 22); POSSIBLY CONSISTENT WITH MASS (RECTUM) NOTED IN CLINICAL OBSERVATIONS THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATE HEART (HT) : -CARDIOMYOPATHY,-SLIGHT THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT DUODENUM (DU) : >AUTOLYTIC JEJUNUM (JE) : >AUTOLYTIC ILEUM (IL) : >AUTOLYTIC CECUM (CE) : >AUTOLYTIC COLON (CO) : >AUTOLYTIC RECTUM (RE) : >AUTOLYTIC SKIN (SK) : -ANGIECTASIS WITH THROMBOSIS AND ENDOTHELIAL PROLIFERATION,-PRESENT EPIDIDYMIDES (EP) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL 2545 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 790 ANIMAL NUMBER: C92766 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/24/00 STUDY DAY OF DEATH: 705 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/24/00 7:57 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -DISCHARGE-OCULAR; RIGHT: DARK RED, DRY MATERIAL CAUSE OF DEATH (CD) : -UNDETERMINED MICROSCOPICALLY,-PRESENT 2546 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 791 ANIMAL NUMBER: C92766 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/24/00 STUDY DAY OF DEATH: 705 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/24/00 7:57 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), HEMATO NEOPLASIA (HN) (SN), 2547 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 792 ANIMAL NUMBER: C92767 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/10/00 STUDY DAY OF DEATH: 691 STUDY WEEK OF DEATH: 99 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/10/00 8:08 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; SORE/SCAB; LATERALABDOMINAL-LEFT; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; OBES; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; VENTRALABDOMINAL-RIGHT; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -NECROSIS, INDIVIDUAL HEPATOCYTE,MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SLIGHT SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE PANCREAS (PA) : -HYPERPLASIA, ISLET CELLS,-MODERATE -B-ADENOMA, ISLET CELL,-NEOPLASM PRESENT ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-MINIMAL -PIGMENT,-SLIGHT ADRENAL, MEDULLA (MA) : -HYPERPLASIA, MEDULLARY,-MINIMAL -B-PHEOCHROMOCYTOMA,-NEOPLASM PRESENT TESTIS (TE) : -ATROPHY/DEGENERATION,-MINIMAL -HYPERPLASIA, INTERSTITIAL CELL (LEYDIG CELL),-MODERATE 2548 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 793 ANIMAL NUMBER: C92767 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/10/00 STUDY DAY OF DEATH: 691 STUDY WEEK OF DEATH: 99 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/10/00 8:08 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,-MODERATE MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,-MODERATE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATELY SEVERE LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL HEART (HT) : -CARDIOMYOPATHY,-SLIGHT -METAPLASIA, OSSEOUS / CARTILAGINOUS,MINIMAL DUODENUM (DU) : >AUTOLYTIC JEJUNUM (JE) : >AUTOLYTIC ILEUM (IL) : >AUTOLYTIC CECUM (CE) : >AUTOLYTIC COLON (CO) : >AUTOLYTIC SKIN (SK) : -ACANTHOSIS/HYPERKERATOSIS,-MODERATELY SEVERE 2549 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 794 ANIMAL NUMBER: C92767 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/10/00 STUDY DAY OF DEATH: 691 STUDY WEEK OF DEATH: 99 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/10/00 8:08 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY SKIN (SK) : -ABRASION(S)/ULCERATION(S); LEFT LATERAL THORACIC REGION: SINGLE MOIST RED ULCERATED AREA, 35 X 25 MM; COLLECTED -CRUSTED AREA(S); RIGHT INGUINAL REGION: SINGLE DRY BROWN CRUSTED AREA, 10 MM IN DIAMETER (CAP 22) LN, LUMBAR/ILIAC (LM) : -LARGE; LEFT: 15 X 11 X 10 MM IS 7 X 4 X 2 MM)(CAP 24) GENERAL COMMENT (GC) : -ANIMAL OBESE (RIGHT SKIN (SK) : -INFLAMMATION, CHRONIC-ACTIVE,MODERATELY SEVERE -ULCERATION,-MODERATE -BACTERIAL COLONIES,-PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL CAUSE OF DEATH (CD) : -INFLAMMATION / INFECTION,-PRESENT LN, LUMBAR/ILIAC (LM) : >UNREMARKABLE 2550 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 795 ANIMAL NUMBER: C92767 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/10/00 STUDY DAY OF DEATH: 691 STUDY WEEK OF DEATH: 99 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/10/00 8:08 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LN, MESENTERIC (MS), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), RECTUM URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) (RE), 2551 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 796 ANIMAL NUMBER: C92768 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/08/00 STUDY DAY OF DEATH: 660 STUDY WEEK OF DEATH: 95 TERMINAL BODY WEIGHT: 944.6 GRAMS DATE AND TIME OF NECROPSY: 02/08/00 8:20 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); DORSAL; WEEK 85; 80 X 100MM; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; VENTRALABDOMINAL-RIGHT; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; BLACK SKIN; DORSAL; VERIFIED AT NECROPSY: NOT OBSERVED OBSERVATIONS NECROPSY TESTIS (TE) : -SMALL; LEFT: 18 X 12 X 4 MM (RIGHT IS 20 X 14 X 8 MM); LEFT IS SOFT HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-SLIGHT -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-SLIGHT -DEGENERATION, CYSTIC,-MINIMAL -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL KIDNEY (KD) : -HYALINE DROPLETS, TUBULAR EPITHELIUM,MODERATELY SEVERE SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : -PIGMENT,-MINIMAL TESTIS (TE) : -ATROPHY/DEGENERATION,-SEVERE >NOTE:>UNILATERAL MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,-MODERATE MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,-MODERATE 2552 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 797 ANIMAL NUMBER: C92768 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/08/00 STUDY DAY OF DEATH: 660 STUDY WEEK OF DEATH: 95 TERMINAL BODY WEIGHT: 944.6 GRAMS DATE AND TIME OF NECROPSY: 02/08/00 8:20 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -RED FOCUS(I)/AREA(S); POSTERIOR ASPECT: SINGLE DARK RED AREA, 1 MM IN DIAMETER SKIN (SK) : -CRUSTED AREA(S); RIGHT VENTRAL ABDOMINAL REGION: SINGLE TAN AND DRY CRUSTED AREA, 1 MM IN DIAMETER (CAP 22) -MASS(ES); DORSAL LUMBAR REGION: SINGLE MASS, 100 X 85 X 60 MM, RED, DARK RED, TAN AND FIRM; SOME SECTIONS EXUDE A TAN FLUID (CAP 23); OVERLYING SKIN HAS A SINGLE DARK RED, TAN AND DRY CRUSTED AREA, 70 X 60 MM; COLLECTED INTACT WITH MASS; REGIONAL LN (INGUINAL) NOT IDENTIFIED THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATELY SEVERE MUSCLE, SKELETAL (SM) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL NERVE, SCIATIC (SN) : >TISSUE MISSING THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT -HYPERPLASIA,-SLIGHT SKIN (SK) : -ACANTHOSIS/HYPERKERATOSIS,-MINIMAL -M-FIBROSARCOMA,-NEOPLASM PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT 2553 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 798 ANIMAL NUMBER: C92768 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/08/00 STUDY DAY OF DEATH: 660 STUDY WEEK OF DEATH: 95 TERMINAL BODY WEIGHT: 944.6 GRAMS DATE AND TIME OF NECROPSY: 02/08/00 8:20 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN EPIDIDYMIDES (EP) : -IMMATURE SPERM FORMS,-MODERATE CAUSE OF DEATH (CD) : -NEOPLASM, SKIN/MUSCLE/SUBCUTIS,PRESENT 2554 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 799 ANIMAL NUMBER: C92768 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/08/00 STUDY DAY OF DEATH: 660 STUDY WEEK OF DEATH: 95 TERMINAL BODY WEIGHT: 944.6 GRAMS DATE AND TIME OF NECROPSY: 02/08/00 8:20 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAr /i LIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), SEMINAL VESICLES (SV), HEMATO NEOPLASIA (HN) 2555 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 800 ANIMAL NUMBER: C92769 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 04/09/00 STUDY DAY OF DEATH: 721 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 04/09/00 8:20 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; LIMITED USE; PAWS-ALL; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) KIDNEY (KD) : -LARGE PELVIS(ES); RIGHT HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-SLIGHT -GRANULAR CYTOPLASM, EOSINOPHILIC, CENTRILOBULAR,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL -DEGENERATION / NECROSIS, CENTRILOBULAR,-SLIGHT KIDNEY (KD) : -DILATATION, PELVIC,-MODERATELY SEVERE -MINERALIZATION, PELVIC,-MINIMAL -NECROSIS, PAPILLARY,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT PANCREAS (PA) : -INFILTRATION, FAT, INTERSTITIAL SLIGHT ADRENAL, CORTEX (AC) -VACUOLATION, FOCAL, -MINIMAL 2556 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 801 ANIMAL NUMBER: C92769 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 04/09/00 STUDY DAY OF DEATH: 721 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 04/09/00 8:20 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); ATTACHED TO LEFT ASPECT: 5 MM IN DIAMETER, RED, TAN AND SOFT STOMACH, GL (ST) : -LIGHT FOCUS(I)/AREA(S); MUCOSA: MULTIPLE GREY AREAS, UP TO 2 X 1 MM THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATELY SEVERE LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL HEART (HT) : -CARDIOMYOPATHY,-SLIGHT MUSCLE, SKELETAL (SM) : -ATROPHY,-SEVERE NERVE, SCIATIC (SN) : -DEGENERATION,-MODERATE THYROID (TY) : -DILATATION / CYST, FOLLICULAR,-PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : >UNREMARKABLE JEJUNUM (JE) : >AUTOLYTIC ILEUM (IL) : >AUTOLYTIC CECUM (CE) : >AUTOLYTIC COLON (CO) : >AUTOLYTIC 2557 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 802 ANIMAL NUMBER: C92769 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 04/09/00 STUDY DAY OF DEATH: 721 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 04/09/00 8:20 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -DISCHARGE-OCULAR; BILATERAL: DRY, RED MATERIAL RECTUM (RE) : >AUTOLYTIC PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 2558 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 803 ANIMAL NUMBER: C92769 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 04/09/00 STUDY DAY OF DEATH: 721 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 04/09/00 8:20 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), SKIN (SK), URINARY BLADDER SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) (UB), 2559 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 804 ANIMAL NUMBER: C92770 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/21/00 STUDY DAY OF DEATH: 702 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/21/00 7:26 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); PAW-FRONT-LEFT; WEEK 97; VERIFIED AT NECROPSY: NOT OBSERVED >APPEARANCE; SWOLLEN; LIMBS-HIND; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; LIMITED USE; LIMBS-ALL; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; YELLOW HAIR COAT; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY ADRENAL, CORTEX (AC) : -LARGE; RIGHT: 8 X 5 X 5 MM (LEFT IS 5 X 3 X 3 MM) HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -GRANULAR CYTOPLASM, EOSINOPHILIC, CENTRILOBULAR,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL -VACUOLATION, HEPATOCELLULAR,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT ADRENAL, CORTEX (AC) : >UNREMARKABLE ADRENAL, MEDULLA (MA) : -B-PHEOCHROMOCYTOMA,- 2 NEOPLASMS PRESENT THYMUS (TH) : >SECTION EXAMINED; TISSUE NOT PRESENT HEART (HT) : -CARDIOMYOPATHY,-MINIMAL MUSCLE, SKELETAL (SM) : -ATROPHY,-MODERATELY SEVERE NERVE, SCIATIC (SN) : -DEGENERATION,-MODERATELY SEVERE 2560 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 805 ANIMAL NUMBER: C92770 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/21/00 STUDY DAY OF DEATH: 702 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/21/00 7:26 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY FOOT/FOOTPAD (FP) : -ABRASION(S)/ULCERATION(S); LEFT HINDFOOT: SINGLE RED, TAN MOIST ABRADED AREA, 4 X 3 MM; COLLECTED; POSSIBLY CONSISTENT WITH (MASS LEFT HINDFOOT) NOTED IN CLINICAL OBSERVATIONS GENERAL COMMENT (GC) : -DISCHARGE-OCULAR; LEFT: RED, BROWN AND MOIST -STAINS-PERINEUM/PERIANAL; YELLOW, BROWN AND MOIST PITUITARY (PI) : -CYST,-PRESENT JEJUNUM (JE) : >AUTOLYTIC ILEUM (IL) : >AUTOLYTIC CECUM (CE) : >AUTOLYTIC COLON (CO) : >AUTOLYTIC RECTUM (RE) : >AUTOLYTIC PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE CAUSE OF DEATH (CD) : -INFLAMMATION / INFECTION,-PRESENT FOOT/FOOTPAD (FP) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE 2561 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 806 ANIMAL NUMBER: C92770 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/21/00 STUDY DAY OF DEATH: 702 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/21/00 7:26 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAr /i LIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2562 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 807 ANIMAL NUMBER: C92771 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/07/99 STUDY DAY OF DEATH: 567 STUDY WEEK OF DEATH: 81 TERMINAL BODY WEIGHT: 684.7 GRAMS DATE AND TIME OF NECROPSY: 11/07/99 7:40 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; ROUGH HAIR COAT; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; RED HAIR COAT; VENTRAL ABDOMINAL; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; RED HAIR COAT; HEADENTIRE; VERIFIED AT NECROPSY: OBSERVED >EYES; RED DISCHARGE; EYES; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >EXCRETION; NO FECES; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -LARGE; ALL LOBES HISTOPATHOLOGY BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-SEVERE -DEGENERATION, CYSTIC,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE, MINIMAL -VACUOLATION, TUBULAR EPITHELIUM,MODERATELY SEVERE SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL -INFILTRATION, FAT, INTERSTITIAL,SLIGHT ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-SLIGHT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATELY SEVERE 2563 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 808 ANIMAL NUMBER: C92771 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/07/99 STUDY DAY OF DEATH: 567 STUDY WEEK OF DEATH: 81 TERMINAL BODY WEIGHT: 684.7 GRAMS DATE AND TIME OF NECROPSY: 11/07/99 7:40 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 10 X 8 X 7 MM, DARK RED AND SOFT STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: MULTIPLE BROWN AREAS, UP TO 2 X 1 MM GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DRY, RED MATERIAL -STAINS - SKIN/HAIRCOAT; ENTIRE VENTRAL SURFACE, EXTENDING TO ENTIRE HEAD: DRY, RED MATERIAL THYROID (TY) : -HYPERTROPHY, FOLLICULAR CELL,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : >UNREMARKABLE STOMACH, NONGL (SU) : -ACANTHOSIS / HYPERKERATOSIS,-SLIGHT -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,MODERATELY SEVERE EPIDIDYMIDES (EP) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 2564 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 809 ANIMAL NUMBER: C92771 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/07/99 STUDY DAY OF DEATH: 567 STUDY WEEK OF DEATH: 81 TERMINAL BODY WEIGHT: 684.7 GRAMS DATE AND TIME OF NECROPSY: 11/07/99 7:40 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), HEMATO NEOPLASIA (HN) 2565 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 810 ANIMAL NUMBER: C92772 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/14/99 STUDY DAY OF DEATH: 360 STUDY WEEK OF DEATH: 52 TERMINAL BODY WEIGHT: 670.3 GRAMS DATE AND TIME OF NECROPSY: 04/14/99 10:00 PROSECTOR: STEVE VAN ADESTINE RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -LARGE; ALL LOBES: MARGINS ARE ROUNDED -MOTTLED; ALL LOBES: RED AND TAN KIDNEY (KD) : -MOTTLED; BILATERAL: BROWN AND TAN HISTOPATHOLOGY LIVER (LI) : -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-MODERATE -CONGESTION,-PRESENT -VACUOLATION, HEPATOCELLULAR,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -PIGMENT, TUBULAR,-SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT THYMUS (TH) : -HEMORRHAGE,-PRESENT -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),SLIGHT LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL HEART (HT) : -CARDIOMYOPATHY,-MINIMAL HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL CAUSE OF DEATH (CD) : -UNDETERMINED MICROSCOPICALLY,-PRESENT 2566 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 811 ANIMAL NUMBER: C92772 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/14/99 STUDY DAY OF DEATH: 360 STUDY WEEK OF DEATH: 52 TERMINAL BODY WEIGHT: 670.3 GRAMS DATE AND TIME OF NECROPSY: 04/14/99 10:00 PROSECTOR: STEVE VAN ADESTINE RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) (TE), 2567 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 812 ANIMAL NUMBER: C92773 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 04/19/00 8:00 PROSECTOR: JODI THIEREN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; SWOLLEN; VENTRAL-ABDOMINALLEFT; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -MOTTLED; ALL LOBES: RED, BROWN AND TAN -MASS(ES); LEFT LATERAL LOBE AND RIGHT LATERAL LOBES: TWO MASSES, BOTH ARE 20 X 20 X 10 MM AND TAN, RED AND FIRM; SAME ON CUT SURFACE; LEFT LATERAL COLLECTED ON ROUTINE SECTION, RIGHT LATERAL COLLECTED AS EXTRA SECTION; REGIONAL LN (HEPATIC) NORMAL (CAP 22) HISTOPATHOLOGY LIVER (LI) : -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MODERATELY SEVERE -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MODERATE -DEGENERATION, CYSTIC,-SLIGHT -HYPERPLASIA, BILE DUCT,-SLIGHT -FIBROSIS, PERIBILIARY,-SLIGHT -HYPERTROPHY, HEPATOCELLULAR, PERIPORTAL,-SLIGHT -DEGENERATION / NECROSIS, CENTRILOBULAR,-SLIGHT -B-ADENOMA, HEPATOCELLULAR,- 2 NEOPLASMS PRESENT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MODERATE SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SLIGHT PANCREAS (PA) : -HYPERTROPHY / BASOPHILIA, ACINAR CELL, MINIMAL ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-SLIGHT 2568 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 813 ANIMAL NUMBER: C92773 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 04/19/00 8:00 PROSECTOR: JODI THIEREN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY LN, MESENTERIC (MS) : -LARGE; UP TO 15 X 10 X 6 MM -DIFFUSELY RED; DARK RED PITUITARY (PI) : >NOTE:>TRIMMING COMMENT: PITUITARY NOT FOUND AT TRIMMING LN, MESENTERIC (MS) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE -FIBROSIS,-MODERATE -ANGIECTASIS,-MODERATELY SEVERE MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,-SLIGHT MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,MODERATELY SEVERE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATE -CYST,-PRESENT THYROID (TY) : -DILATATION / CYST, FOLLICULAR,-PRESENT -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : >TISSUE MISSING HARDERIAN GLAND (HG) : -DYSPLASIA, EPITHELIAL, GLANDULAR,SLIGHT JEJUNUM (JE) : >AUTOLYTIC CECUM (CE) : -HEMORRHAGE,-MODERATE -INFLAMMATION, CHRONIC-ACTIVE,MODERATELY SEVERE 2569 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 814 ANIMAL NUMBER: C92773 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 04/19/00 8:00 PROSECTOR: JODI THIEREN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY CECUM (CE) : -MASS(ES); MID PORTION OF CECUM: SINGLE MASS, 65 X 50 X 35 MM, RED, DARK RED, TAN, FIRM, AND VASCULAR; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (SEE MESENTERIC); POSSIBLY CONSISTENT WITH SWOLLEN (ABDOMINALVENTRAL-LEFT) AS NOTED IN CLINICAL OBSERVATIONS LN, LUMBAR/ILIAC (LM) : -MOTTLED; BILATERAL: DARK RED AND TAN (CAP 25) CECUM (CE) : -BACTERIA,-PRESENT -ULCERATION,-MODERATELY SEVERE -B-LIPOMA,-NEOPLASM PRESENT COLON (CO) : >AUTOLYTIC PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL CAUSE OF DEATH (CD) : -NEOPLASM, ABDOMINAL CAVITY,-PRESENT LN, OTHER (LN) : -INFILTRATE, MACROPHAGE, PIGMENTED,SLIGHT >NOTE:>HEPATIC LN LN, LUMBAR/ILIAC (LM) : -LYMPHANGIECTASIS,-PRESENT -HYPERPLASIA, LYMPHOPLASMACYTIC,MINIMAL -HEMORRHAGE,-PRESENT CAVITY, ABDOM (PC) : -HEMATOMA,-PRESENT 2570 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 815 ANIMAL NUMBER: C92773 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 04/19/00 8:00 PROSECTOR: JODI THIEREN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY CAVITY, ABDOM (PC) : -MASS(ES); ATTACHED TO ADIPOSE TISSUE NEAR HEAD OF RIGHT EPIDIDYMIS: SINGLE MASS, 23 X 13 X 5 MM, RED, DARK RED, FIRM AND LOBULATED; SAME ON CUT SURFACE (CAP 24); REGIONAL LN (SEE LUMBAR) GENERAL COMMENT (GC) : -GI TRACT-LARGE LUMEN; CECUM AND COLON: DISTENDED UP TO 13 MM; FILLED WITH A DARK RED FLUID -FLUID-ABDOMINAL CAVITY; FILLED WITH A RED FLUID -GI TRACT-DARK FLUID CONTENT; JEJUNUM, ILEUM, CECUM AND COLON: FILLED WITH A DARK RED FLUID 2571 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 816 ANIMAL NUMBER: C92773 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 04/19/00 8:00 PROSECTOR: JODI THIEREN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), ADRENAL, MEDULLA (MA), TESTIS (TE), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), ILEUM (IL), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2572 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 817 ANIMAL NUMBER: C92774 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 767.6 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 9:43 PROSECTOR: TROY ELLENBECKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) KIDNEY (KD) : -ROUGH SURFACE, DIFFUSE; BILATERAL HISTOPATHOLOGY BRAIN (BR) : >UNREMARKABLE LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-SLIGHT -NECROSIS, INDIVIDUAL HEPATOCYTE,MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-SLIGHT -DEGENERATION, CYSTIC,-SLIGHT -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL -FIBROSIS, PERIBILIARY,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MODERATELY SEVERE SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE PANCREAS (PA) : -ATROPHY, ACINAR,-SLIGHT ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-MINIMAL ADRENAL, MEDULLA (MA) : -B-PHEOCHROMOCYTOMA,-NEOPLASM PRESENT 2573 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 818 ANIMAL NUMBER: C92774 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 767.6 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 9:43 PROSECTOR: TROY ELLENBECKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 7 X 6 X 4 MM, RED, DARK RED, TAN, AND SOFT STOMACH, GL (ST) : -THICKENED WALL; MUCOSA: DIFFUSE PROSTATE (PR) : -NODULAR; PROXIMAL SURROUNDING URETHRA: GREEN AND FIRM GENERAL COMMENT (GC) : -BLOOD FILM TAKEN TESTIS (TE) : -ATROPHY/DEGENERATION,-SEVERE >NOTE:>UNILATERAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATELY SEVERE LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL MUSCLE, SKELETAL (SM) : -DEGENERATION,-MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL -B-ADENOMA, FOLLICULAR CELL,-NEOPLASM PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : >UNREMARKABLE PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2574 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 819 ANIMAL NUMBER: C92774 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 767.6 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 9:43 PROSECTOR: TROY ELLENBECKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2575 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 820 ANIMAL NUMBER: C92775 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 638.8 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 7:38 PROSECTOR: TODD ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); DORSAL; WEEK 72; 10 X 10MM; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; DORSAL; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY LIVER (LI) : -LIGHT FOCUS(I)/AREA(S); DIAPHRAGMATIC SURFACE OF LEFT LATERAL LOBE: SINGLE TAN FOCUS, 2 MM IN DIAMETER (CAP 22) KIDNEY (KD) : -ROUGH SURFACE, DIFFUSE; BILATERAL -LARGE; BILATERAL: EACH IS 30 X 20 X 15 MM SPLEEN (SP) : -LARGE; 60 X 16 X 8 MM HISTOPATHOLOGY EYE (EY) : -DYSTROPHY, CORNEAL IN EARLY STAGES, CONSISTS PRIMARILY OF FOCAL MINERALIZATION,-MINIMAL BRAIN (BR) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT LIVER (LI) : -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MODERATE -DEGENERATION, CYSTIC,-SLIGHT -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT LIVER-OIL RED O (LI0) : -VACUOLES, OIL RED O-POSITIVE, HEPATOCELLULAR,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SEVERE SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SLIGHT -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT 2576 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 821 ANIMAL NUMBER: C92775 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 638.8 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 7:38 PROSECTOR: TODD ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY ADRENAL, CORTEX (AC) : -LARGE; LEFT: 10 X 8 X 6 MM (RIGHT IS 6 X 5 X 5 MM) TESTIS (TE) : -SMALL; RIGHT: 20 X 11 X 7 MM (LEFT IS 21 X 15 X 11 MM) PANCREAS (PA) : -M-CARCINOMA, ISLET CELL,-NEOPLASM PRESENT ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-MINIMAL -HYPERTROPHY, CORTICAL CELL,-MINIMAL -DEGENERATION, CYSTIC / HEMORRHAGIC,SLIGHT -HYPERPLASIA, CORTICAL,-MINIMAL -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT ADRENAL, MEDULLA (MA) : -B-PHEOCHROMOCYTOMA,- 2 NEOPLASMS PRESENT LN, MESENTERIC (MS) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT TESTIS (TE) : -ATROPHY/DEGENERATION,-SEVERE >NOTE:>UNILATERAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATELY SEVERE LUNG (LU) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT -HEMORRHAGE,-PRESENT 2577 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 822 ANIMAL NUMBER: C92775 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 638.8 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 7:38 PROSECTOR: TODD ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY THYROID (TY) : -LARGE; BILATERAL: EACH IS 7 X 4 X 3 MM PARATHYROID (PT) : -LARGE; BILATERAL: EACH IS 3 X 2 X 1 MM PITUITARY (PI) : -CYST(S); LEFT ANTERIOR ASPECT: SINGLE CLEAR FLUID FILLED CYST, 1 MM IN DIAMETER STOMACH, GL (ST) : -THICKENED WALL; MUCOSA: DIFFUSE LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL HEART (HT) : -CARDIOMYOPATHY,-MODERATE -MINERALIZATION, VASCULAR,-MODERATELY SEVERE THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL -DILATATION / CYST, FOLLICULAR,-PRESENT PARATHYROID (PT) : -HYPERPLASIA,-MODERATELY SEVERE PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : -HYPERPLASIA, EPITHELIAL, GLANDULAR,MODERATE -MINERALIZATION, GLANDULAR MUCOSA,MODERATELY SEVERE SKIN (SK) : -B-KERATOACANTHOMA,-NEOPLASM PRESENT -EDEMA,-SEVERE 2578 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 823 ANIMAL NUMBER: C92775 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 638.8 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 7:38 PROSECTOR: TODD ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY SKIN (SK) : -MASS #2; LEFT LATERAL THORACIC REGION: SINGLE MASS, 15 X 15 X 8 MM, TAN AND FIRM; CUT SURFACE EXUDES A TAN CASEOUS SEMISOLID; COLLECTED; OVERLYING SKIN HAS A SINGLE DARK RED AND DRY CRUSTED AREA, 5 X 4 MM; COLLECTED INTACT WITH MASS; REGIONAL LN (AXILLARY) NORMAL (CAP 25) -SUBCUTANEOUS EDEMA; VENTRAL CERVICAL REGION: CLEAR AND GELATINOUS (CAP 23) PROSTATE (PR) : -GELATINOUS; DISTAL PORTION: CLEAR LN, RENAL (RL) : -LARGE; LEFT: 8 X 6 X 4 MM (RIGHT IS 5 X 4 X 2 MM) (CAP 24) -DIFFUSELY RED; BILATERAL: DARK RED (CAP 24) PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SEVERE SEMINAL VESICLES (SV) : -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL EPIDIDYMIDES (EP) : -HYPOSPERMIA,-PRESENT -VACUOLATION, TUBULAR EPITHELIUM,SLIGHT HEMATO NEOPLASIA (HN) : -M-LYMPHOMA,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, RENAL (RL) : -HEMORRHAGE,-PRESENT -DILATATION, SINUSOIDAL,-SEVERE 2579 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 824 ANIMAL NUMBER: C92775 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 638.8 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 7:38 PROSECTOR: TODD ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY AORTA (AO) : -LARGE; DIFFUSE: UP TO 6 MM IN DIAMETER; COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN AORTA (AO) : -MINERALIZATION,-MODERATE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), HARDERIAN GLAND (HG), SALIV STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), LN, AXILLARY (AX) GL, MANDIB (SG), 2580 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 825 ANIMAL NUMBER: C92776 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 747.8 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 10:50 PROSECTOR: JARED FISHEL RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >WART LIKE LESION(S); MOUTH; WEEK 102; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -LIGHT FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE TAN, SLIGHTLY RAISED AREAS, UP TO 8 MM IN DIAMETER; COLLECTED ON ROUTINE SECTIONS KIDNEY (KD) : -MASS(ES); LEFT, CORTICAL SURFACE EXTENDING INTO MEDULLA: SINGLE MASS, 10 MM IN DIAMETER, TAN AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (SEE RENAL) HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-MODERATE -GRANULAR CYTOPLASM, EOSINOPHILIC, CENTRILOBULAR,-SLIGHT -PIGMENT, HEPATOCELLULAR, CENTRILOBULAR,-MINIMAL -VACUOLATION, HEPATOCELLULAR, MIDZONAL / CENTRILOBULAR,-MODERATELY SEVERE -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL -DEGENERATION, CYSTIC,-SLIGHT -HYPERPLASIA, BILE DUCT,-MINIMAL -B-ADENOMA, HEPATOCELLULAR,-NEOPLASM PRESENT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT -B-LIPOMA,-NEOPLASM PRESENT 2581 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 826 ANIMAL NUMBER: C92776 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 747.8 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 10:50 PROSECTOR: JARED FISHEL RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY SKIN (SK) : -MASS(ES); RIGHT MAXILLARY REGION: SINGLE MASS, 4 X 2 X 2 MM, TAN AND FIRM (CAP 22); REGIONAL LN (MANDIBULAR) NORMAL (CAP 23) PANCREAS (PA) : -INFILTRATION, FAT, INTERSTITIAL,SLIGHT ADRENAL, CORTEX (AC) : -HYPERTROPHY, CORTICAL CELL,-MINIMAL -PIGMENT,-MINIMAL ADRENAL, MEDULLA (MA) : -UNILATERALLY EXAMINED,-PRESENT THYMUS (TH) : >SECTION EXAMINED; TISSUE NOT PRESENT LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL HEART (HT) : -CARDIOMYOPATHY,-MINIMAL THYROID (TY) : -B-ADENOMA, FOLLICULAR CELL,-NEOPLASM PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT -HYPERPLASIA,-MINIMAL SKIN (SK) : ^UNREMARKABLE URINARY BLADDER (UB) : -PROTEINACEOUS MATERIAL, LUMINAL,PRESENT 2582 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 827 ANIMAL NUMBER: C92776 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 747.8 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 10:50 PROSECTOR: JARED FISHEL RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY LN, RENAL (RL) : -LARGE; LEFT: 6 MM IN DIAMETER, RIGHT IS 3 X 2 X 1 MM (CAP 25) MAMMARY, MALE (MM) : -MASS(ES); LEFT INGUINAL REGION: SINGLE MASS, 25 X 25 X 10 MM, TAN, FIRM AND LOBULATED; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE SEMINAL VESICLES (SV) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, RENAL (RL) : >NOT REQUIRED TO BE EXAMINED FOR ANIMAL >NOTE:>NO LYMPH NODE PRESENT MASSES APPEAR TO BE PART OF THE KIDNEY NEOPLASM LN, MANDIBULAR (MN) : -HYPERPLASIA, LYMPHOPLASMACYTIC,MODERATELY SEVERE -HEMORRHAGE,-PRESENT -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL MAMMARY, MALE (MM) : -B-FIBROADENOMA,-NEOPLASM PRESENT 2583 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 828 ANIMAL NUMBER: C92776 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 747.8 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 10:50 PROSECTOR: JARED FISHEL RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, MANDIBULAR (MN), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2584 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 829 ANIMAL NUMBER: C92777 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 425.4 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 13:15 PROSECTOR: DONIA MEYER RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT' (%) LF ADRENAL (AD0) RT ADRENAL (AD1) BRAIN (BR) LF KIDNEY (KD0) RT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LF TESTIS (TE0) RT TESTIS (TE1) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) .0202 .0180 1.8515 2.0608 2.1126 20.8174 1.5024 .6408 1.5350 1.5449 .0136 .0106 .0047 % .0042 % .4352 % .4844 % .4966 % 4.8936 % .3532 % .1506 % .3608 % .3632 % .0032 % .0025 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0109 .0097 1.0000 1.1130 1.1410 11.2435 .8115 .3461 .8291 .8344 .0073 .0057 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-MODERATE -NECROSIS, INDIVIDUAL HEPATOCYTE,MINIMAL -VACUOLATION, HEPATOCELLULAR, MIDZONAL / CENTRILOBULAR,-SLIGHT KIDNEY (KD) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -REGENERATION, TUBULAR EPITHELIUM,MINIMAL 2585 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 830 ANIMAL NUMBER: C92777 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 425.4 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 13:15 PROSECTOR: DONIA MEYER RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN LN, MESENTERIC (MS) : -HEMORRHAGE,-PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), TESTIS (TE) 2586 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 831 ANIMAL NUMBER: C92778 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 676.5 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 9:35 PROSECTOR: NANCY DIEDRICH RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT' (%) LF ADRENAL (AD0) RT ADRENAL (AD1) BRAIN (BR) LF KIDNEY (KD0) RT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LF TESTIS (TE0) RT TESTIS (TE1) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) .0286 .0286 2.3638 2.7978 2.6827 30.0439 2.2742 .9348 1.0030 1.0456 .0242 .0422 .0042 % .0042 % .3494 % .4136 % .3966 % 4.4411 % .3362 % .1382 % .1483 % .1546 % .0036 % .0062 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0121 .0121 1.0000 1.1836 1.1349 12.7100 .9621 .3955 .4243 .4423 .0102 .0179 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED LIVER (LI) : -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-MODERATE -VACUOLATION, HEPATOCELLULAR, MIDZONAL / CENTRILOBULAR,-MODERATE KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MINIMAL 2587 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 832 ANIMAL NUMBER: C92778 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 676.5 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 9:35 PROSECTOR: NANCY DIEDRICH RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY TESTIS (TE) : -SMALL; BILATERAL: EACH IS 18 X 13 X 8 MM; BOTH ARE SOFT GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,SLIGHT ADRENAL, CORTEX (AC) : -HYPERTROPHY, CORTICAL CELL,-MINIMAL TESTIS (TE) : >UNREMARKABLE EPIDIDYMIDES (EP) : -IMMATURE SPERM FORMS,-MINIMAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2588 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 833 ANIMAL NUMBER: C92778 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 676.5 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 9:35 PROSECTOR: NANCY DIEDRICH RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), HEMATO NEOPLASIA (HN) (SM), 2589 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 834 ANIMAL NUMBER: C92779 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 274.5 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 8:36 PROSECTOR: LYNN HORNER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN STATUS LF ADRENAL (AD0) RT ADRENAL (AD1) BRAIN (BR) LF KIDNEY (KD0) RT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LF TESTIS (TE0) RT TESTIS (TE1) LF THYROID/PARA RT THYROID/PARA (TT0) (TT1) ------------------------------------ 11.8757 ------------------------------------------- ------------------------------------ 4.3268 % ------------------------------------------- ------------------------------------------------------------------------------------- NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN WEIGHT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS 2590 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 835 ANIMAL NUMBER: C92779 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 274.5 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 8:36 PROSECTOR: LYNN HORNER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) 2591 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 836 ANIMAL NUMBER: C92780 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 515.3 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 10:30 PROSECTOR: STEVE VAN ADESTINE RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; HUNCHED; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -LIGHT FOCUS(I)/AREA(S); DIAPHRAGMATIC SURFACE OF RIGHT MEDIAN LOBE: SINGLE TAN AREA, 5 X 3 MM; COLLECTED ON ROUTINE SECTION -ROUGH SURFACE, DIFFUSE; ALL LOBES HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-SLIGHT -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-MODERATE -GRANULAR CYTOPLASM, EOSINOPHILIC, CENTRILOBULAR,-SLIGHT -NECROSIS, INDIVIDUAL HEPATOCYTE,MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-MODERATE -FIBROSIS, PERIBILIARY,-MODERATE -B-ADENOMA, HEPATOCELLULAR,-NEOPLASM PRESENT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL 2592 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 837 ANIMAL NUMBER: C92780 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 515.3 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 10:30 PROSECTOR: STEVE VAN ADESTINE RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 10 X 8 X 7 MM, RED, DARK RED, AND SOFT PROSTATE (PR) : -NODULAR; PROXIMAL SURROUNDING URETHRA: GREEN AND FIRM SPLEEN (SP) : -PIGMENT, INCREASED,-MINIMAL -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MINIMAL PANCREAS (PA) : -INFILTRATION, FAT, INTERSTITIAL,MODERATE ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-MODERATE -PIGMENT,-MODERATE -B-ADENOMA,-NEOPLASM PRESENT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATELY SEVERE LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL -HYPERPLASIA, TYPE II PNEUMOCYTE,MINIMAL HEART (HT) : -CARDIOMYOPATHY,-SLIGHT THYROID (TY) : -HYPERPLASIA, C-CELL,-SLIGHT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL 2593 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 838 ANIMAL NUMBER: C92780 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 515.3 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 10:30 PROSECTOR: STEVE VAN ADESTINE RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY SEMINAL VESICLES (SV) : -GELATINOUS; BILATERAL: YELLOW AND CLEAR SEMINAL VESICLES (SV) : >UNREMARKABLE CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG), GENERAL COMMENT (GC) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2594 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 839 ANIMAL NUMBER: C92781 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/06/99 STUDY DAY OF DEATH: 535 STUDY WEEK OF DEATH: 77 TERMINAL BODY WEIGHT: 356.5 GRAMS DATE AND TIME OF NECROPSY: 10/06/99 14:08 PROSECTOR: JODI THIEREN RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; PALE; ENTIRE BODY; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; COLD TO TOUCH; ENTIRE BODY; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; TREMORS; ENTIRE BODY; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) SPLEEN (SP) : -SMALL; 30 X 8 X 44 MM TESTIS (TE) : -SMALL; BILATERAL: EACH IS 20 X 9 X 5 MM; BOTH ARE SOFT HISTOPATHOLOGY BRAIN (BR) : >UNREMARKABLE LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-SLIGHT -PIGMENT, HEPATOCELLULAR, CENTRILOBULAR,-MINIMAL -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL ADRENAL, CORTEX (AC) : -PIGMENT,-MODERATE -ATROPHY, CORTICAL,-MODERATELY SEVERE TESTIS (TE) : -ATROPHY/DEGENERATION,-SEVERE 2595 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 840 ANIMAL NUMBER: C92781 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/06/99 STUDY DAY OF DEATH: 535 STUDY WEEK OF DEATH: 77 TERMINAL BODY WEIGHT: 356.5 GRAMS DATE AND TIME OF NECROPSY: 10/06/99 14:08 PROSECTOR: JODI THIEREN RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 7 X 7 X 6 MM, TAN AND FIRM PROSTATE (PR) : -SMALL; 15 X 8 X 4 MM SEMINAL VESICLES (SV) : -SMALL; BILATERAL: EACH IS 13 X 7 X 2 MM EPIDIDYMIDES (EP) : -SMALL; BILATERAL: BOTH ARE 40 X 5 X 4 MM THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),SLIGHT PARATHYROID (PT) : >SECTION EXAMINED; TISSUE NOT PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-SLIGHT -ATROPHY,-MODERATE STOMACH, GL (ST) : -EROSION,-MINIMAL -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE -HYPERPLASIA, EPITHELIAL, GLANDULAR,MODERATE PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,MODERATELY SEVERE -ATROPHY,-MODERATELY SEVERE SEMINAL VESICLES (SV) : -ATROPHY,-MODERATELY SEVERE EPIDIDYMIDES (EP) : -ATROPHY/DEGENERATION,-MODERATELY SEVERE CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 2596 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 841 ANIMAL NUMBER: C92781 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/06/99 STUDY DAY OF DEATH: 535 STUDY WEEK OF DEATH: 77 TERMINAL BODY WEIGHT: 356.5 GRAMS DATE AND TIME OF NECROPSY: 10/06/99 14:08 PROSECTOR: JODI THIEREN RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAr /i LIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), HEMATO NEOPLASIA (HN) 2597 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 842 ANIMAL NUMBER: C92782 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 972.9 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 12:57 PROSECTOR: JAMES T. CALLEN RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); VENTRAL-ABDOMINAL-RIGHT; WEEK 103; VERIFIED AT NECROPSY: NOT OBSERVED >SKIN & PELAGE; SORE/SCAB; VENTRALABDOMINAL-RIGHT; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE DARK RED AREAS, UP TO 4 X 3 MM; SOME ARE SUNKEN WITH A STELLATE PATTERN; COLLECTED ON ROUTINE SECTIONS HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-MINIMAL -DEGENERATION, CYSTIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE PANCREAS (PA) : -B-ADENOMA, ISLET CELL,-NEOPLASM PRESENT ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-SLIGHT ADRENAL, MEDULLA (MA) : -HYPERPLASIA, MEDULLARY,-MINIMAL 2598 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 843 ANIMAL NUMBER: C92782 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 972.9 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 12:57 PROSECTOR: JAMES T. CALLEN RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY 6PITUITARY (PI) : -MASS(ES); X 6 X 4 MM, TAN AND FIRM STOMACH, GL (ST) -THICKENED WALL; ALL REGIONS:: DIFFUSE SKIN (SK) : -CRUSTED AREA(S); MID VENTRAL ABDOMINAL REGION: SINGLE DRY, RED CRUSTED AREA, 4 X 2 MM (CAP 22) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATELY SEVERE LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL HEART (HT) : -CARDIOMYOPATHY,-SLIGHT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : >UNREMARKABLE RECTUM (RE) : -PARASITISM,-PRESENT SKIN (SK) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE -SUPERFICIAL CRUSTING,-SLIGHT -FIBROSIS,-MODERATE URINARY BLADDER (UB) : -HYPERPLASIA, TRANSITIONAL CELL,-SLIGHT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2599 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 844 ANIMAL NUMBER: C92782 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 972.9 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 12:57 PROSECTOR: JAMES T. CALLEN RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -URINARY BLADDER-LARGE LUMEN; X 15 MM -ANIMAL OBESE 25 X 18 THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2600 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 845 ANIMAL NUMBER: C92783 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/18/99 STUDY DAY OF DEATH: 547 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: 733.9 GRAMS DATE AND TIME OF NECROPSY: 10/18/99 12:04 PROSECTOR: STEVE VAN ADESTINE RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >EYES; RED DISCHARGE; EYE-RIGHT; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: NOT OBSERVED >EXCRETION; NO FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >RESPIRATION; AUDIBLE; VERIFIED AT NECROPSY: NOT APPLICABLE >RESPIRATION; LABORED; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; COLD TO TOUCH; ENTIRE BODY; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; RECUMBENT; STERNAL; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY LIVER (LI) : -LIGHT FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE TAN, SLIGHTLY RAISED AREAS, UP TO 20 X 12 MM; COLLECTED ON ROUTINE SECTIONS KIDNEY (KD) : -DIFFUSELY LIGHT; BILATERAL: TAN TESTIS (TE) : -SMALL; RIGHT: 20 X 10 X 4 MM (LEFT IS 24 X 12 X 10 MM) HISTOPATHOLOGY EYE (EY) : -INFLAMMATION, ACUTE,-MINIMAL BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -HEMORRHAGE,-MINIMAL LIVER (LI) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SLIGHT -HYALINE DROPLETS, TUBULAR EPITHELIUM,MODERATE SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SLIGHT ADRENAL, MEDULLA (MA) : -B-PHEOCHROMOCYTOMA,-NEOPLASM PRESENT TESTIS (TE) : -ATROPHY/DEGENERATION,-SEVERE >NOTE:>UNILATERAL 2601 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 846 ANIMAL NUMBER: C92783 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/18/99 STUDY DAY OF DEATH: 547 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: 733.9 GRAMS DATE AND TIME OF NECROPSY: 10/18/99 12:04 PROSECTOR: STEVE VAN ADESTINE RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY LUNG (LU) : -DARK FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE DARK BROWN AREAS, UP TO 3 X 2 MM PITUITARY (PI) : -MASS(ES); 12 X 10 X 8 MM, RED, GRAY, AND SOFT STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: MULTIPLE DARK BROWN AREAS, UP TO 3 X 2 MM -RED FOCUS(I)/AREA(S); MUCOSA: MULTIPLE AREAS, UP TO 7 X 4 MM CECUM (CE) : -RED FOCUS(I)/AREA(S); MUCOSA: MULTIPLE DARK RED AREAS, UP TO 13 X 6 MM PROSTATE (PR) : -NODULAR; PROXIMAL, SURROUNDING URETHRA: GREEN AND FIRM THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATELY SEVERE LUNG (LU) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT -MINERALIZATION, VASCULAR,-MINIMAL -HEMORRHAGE,-PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : -EDEMA,-MODERATE -EROSION,-MINIMAL -MINERALIZATION, GLANDULAR MUCOSA,MINIMAL -FIBROSIS,-MODERATE CECUM (CE) : -EROSION,-SLIGHT -HEMORRHAGE,-SLIGHT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT HEMATO NEOPLASIA (HN) : -M-SARCOMA, HISTIOCYTIC,-NEOPLASM PRESENT 2602 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 847 ANIMAL NUMBER: C92783 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/18/99 STUDY DAY OF DEATH: 547 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: 733.9 GRAMS DATE AND TIME OF NECROPSY: 10/18/99 12:04 PROSECTOR: STEVE VAN ADESTINE RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; RIGHT: DARK RED, MOIST MATERIAL CAUSE OF DEATH (CD) : -NEOPLASM, HEMATOPOIETIC,-PRESENT 2603 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 848 ANIMAL NUMBER: C92783 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/18/99 STUDY DAY OF DEATH: 547 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: 733.9 GRAMS DATE AND TIME OF NECROPSY: 10/18/99 12:04 PROSECTOR: STEVE VAN ADESTINE RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAr /i LIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), ADRENAL, CORTEX (AC), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP) 2604 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 849 ANIMAL NUMBER: C92784 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/27/99 STUDY DAY OF DEATH: 556 STUDY WEEK OF DEATH: 80 TERMINAL BODY WEIGHT: 576.2 GRAMS DATE AND TIME OF NECROPSY: 10/27/99 8:28 PROSECTOR: JARED FISHEL RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >EXCRETION; NONFORMED FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) HISTOPATHOLOGY EYE (EY) : -DYSTROPHY, CORNEAL IN EARLY STAGES, CONSISTS PRIMARILY OF FOCAL MINERALIZATION,-MINIMAL BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT LIVER (LI) : -DEGENERATION, CYSTIC,-MINIMAL -GLYCOGEN, HEPATOCELLULAR, INCREASED,SEVERE KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-MODERATE -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL -INFILTRATION, FAT, INTERSTITIAL,SLIGHT ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC / HEMORRHAGIC,SLIGHT -PIGMENT,-MINIMAL 2605 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 850 ANIMAL NUMBER: C92784 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/27/99 STUDY DAY OF DEATH: 556 STUDY WEEK OF DEATH: 80 TERMINAL BODY WEIGHT: 576.2 GRAMS DATE AND TIME OF NECROPSY: 10/27/99 8:28 PROSECTOR: JARED FISHEL RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 9 X 9 X 8 MM, DARK RED, SOFT, AND LOBULATED STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA: MULTIPLE TAN ERODED AREAS, UP TO 3 MM IN DIAMETER PROSTATE (PR) : -NODULAR; PROXIMAL, SURROUNDING URETHRA: GREEN AND FIRM GENERAL COMMENT (GC) : -BLOOD FILM TAKEN THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATELY SEVERE LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL HEART (HT) : -CARDIOMYOPATHY,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, NONGL (SU) : -ACANTHOSIS / HYPERKERATOSIS,-MINIMAL -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT URINARY BLADDER (UB) : -HEMORRHAGE,-MINIMAL -HYPERPLASIA, TRANSITIONAL CELL,MODERATE -INFILTRATE, MACROPHAGE, PIGMENTED,SLIGHT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 2606 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 851 ANIMAL NUMBER: C92784 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/27/99 STUDY DAY OF DEATH: 556 STUDY WEEK OF DEATH: 80 TERMINAL BODY WEIGHT: 576.2 GRAMS DATE AND TIME OF NECROPSY: 10/27/99 8:28 PROSECTOR: JARED FISHEL RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2607 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 852 ANIMAL NUMBER: C92785 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/05/00 STUDY DAY OF DEATH: 686 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/05/00 13:43 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY KIDNEY (KD) : -LARGE PELVIS(ES); BILATERAL -MOTTLED; BILATERAL: RED AND TAN HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-MINIMAL -HYPERPLASIA, BILE DUCT,-SLIGHT -FIBROSIS, PERIBILIARY,-SLIGHT KIDNEY (KD) : -DILATATION, PELVIC,-SLIGHT -MINERALIZATION, TUBULAR,-MODERATE SPLEEN (SP) : -PIGMENT, INCREASED,-MINIMAL PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-SLIGHT ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT -HYPERPLASIA, CORTICAL,-MINIMAL SPINAL CORD (SC) : >UNREMARKABLE >NOTE:>2 SECTIONS EXAMINED THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATELY SEVERE 2608 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 853 ANIMAL NUMBER: C92785 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/05/00 STUDY DAY OF DEATH: 686 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/05/00 13:43 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -CYST(S); LEFT ASPECT: SINGLE CYST, 4 MM IN DIAMETER, FILLED WITH A DARK RED FLUID URINARY BLADDER (UB) : -RED FOCUS(I)/AREA(S); MUCOSA: MULTIPLE DARK RED AREAS, UP TO 4 X 6 MM LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL (CAP 22) GENERAL COMMENT (GC) : -DISCHARGE-OCULAR; BILATERAL: DRY RED MATERIAL LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL HEART (HT) : -CARDIOMYOPATHY,-MODERATE MUSCLE, SKELETAL (SM) : -ATROPHY,-MODERATE THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : -MINERALIZATION, VASCULAR,-MINIMAL URINARY BLADDER (UB) : >AUTOLYTIC PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE SEMINAL VESICLES (SV) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT CAUSE OF DEATH (CD) : -UNDETERMINED MICROSCOPICALLY,-PRESENT LN, MANDIBULAR (MN) : -HEMORRHAGE,-PRESENT 2609 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 854 ANIMAL NUMBER: C92785 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/05/00 STUDY DAY OF DEATH: 686 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/05/00 13:43 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -DISCHARGE-NASAL; DRY RED MATERIAL -URINARY BLADDER-LARGE LUMEN; 22 X 22 X 40 MM, FILLED WITH A DARK RED FLUID -STAINS - SKIN/HAIRCOAT; ENTIRE VENTRAL SURFACE: DRY BROWN MATERIAL 2610 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 855 ANIMAL NUMBER: C92785 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/05/00 STUDY DAY OF DEATH: 686 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/05/00 13:43 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2611 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 856 ANIMAL NUMBER: C92786 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 829.4 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 11:16 PROSECTOR: DAVID SCHUETTE RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -LIGHT FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE TAN AREAS, UP TO 4 MM IN DIAMETER; COLLECTED ON ROUTINE SECTIONS HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-MODERATELY SEVERE -VACUOLATION, HEPATOCELLULAR, MIDZONAL / CENTRILOBULAR,-MODERATELY SEVERE -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MODERATE -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MODERATE LIVER-OIL RED O (LI0) : -VACUOLES, OIL RED O-POSITIVE, HEPATOCELLULAR,-MODERATE KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MODERATE SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SLIGHT PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,MODERATELY SEVERE -M-CARCINOMA, ISLET CELL,-NEOPLASM PRESENT 2612 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 857 ANIMAL NUMBER: C92786 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 829.4 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 11:16 PROSECTOR: DAVID SCHUETTE RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PANCREAS (PA) : -MASS(ES); ALONG DUODENUM: SINGLE MASS, 16 X 12 X 8 MM, RED, DARK RED, TAN, YELLOW, FIRM, AND LOBULATED; SAME ON CUT SURFACE (CAP 22); REGIONAL LN (ANTERIOR MESENTERIC) NORMAL (CAP 23) STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: SINGLE DARK BROWN AREA, 4 X 2 MM -THICKENED WALL; DIFFUSE GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -ANIMAL OBESE ADRENAL, CORTEX (AC) : -PIGMENT,-SLIGHT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MINIMAL HEART (HT) : -CARDIOMYOPATHY,-SLIGHT PARATHYROID (PT) : -HYPERPLASIA,-MODERATE PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT -HYPERPLASIA,-MINIMAL STOMACH, GL (ST) : -HYPERPLASIA, EPITHELIAL, GLANDULAR,SLIGHT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2613 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 858 ANIMAL NUMBER: C92786 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 829.4 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 11:16 PROSECTOR: DAVID SCHUETTE RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUm /s KULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN), LN, ANT MES/PANC (AP) 2614 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 859 ANIMAL NUMBER: C92787 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/13/99 STUDY DAY OF DEATH: 328 STUDY WEEK OF DEATH: 47 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/13/99 10:30 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; LIMITED USE; LIMBS-HIND; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY KIDNEY (KD) : -LARGE PELVIS(ES); BILATERAL HISTOPATHOLOGY LIVER (LI) : -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-SLIGHT -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-MODERATE -DEGENERATION / NECROSIS, MIDZONAL,MODERATE KIDNEY (KD) : -DILATATION, PELVIC,-SLIGHT -REGENERATION, TUBULAR EPITHELIUM,MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SLIGHT SPINAL CORD (SC) : -HEMORRHAGE,-SLIGHT THYMUS (TH) : -HEMORRHAGE,-PRESENT -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATELY SEVERE LUNG (LU) : -CONGESTION,-PRESENT 2615 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 860 ANIMAL NUMBER: C92787 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/13/99 STUDY DAY OF DEATH: 328 STUDY WEEK OF DEATH: 47 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/13/99 10:30 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY SKIN (SK) : -ABRASION(S)/ULCERATION(S); MID ABDOMINAL REGION: SINGLE RED AND TAN ULCERATED AREA, 10 MM IN DIAMETER (CAP 21) URINARY BLADDER (UB) : -CALCULUS(I); WITHIN LUMEN: MULTIPLE TAN AND FIRM CALCULI, UP TO 4 MM IN DIAMETER LN, MANDIBULAR (MN) : -LARGE; RIGHT: 8 X 5 X 2 MM (FOR COMPARISON LEFT IS 4 X 2 X 1 MM) MUSCLE, SKELETAL (SM) : -ATROPHY,-MODERATE PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT DUODENUM (DU) : >AUTOLYTIC ILEUM (IL) : >AUTOLYTIC CECUM (CE) : >AUTOLYTIC COLON (CO) : >AUTOLYTIC SKIN (SK) : -ACANTHOSIS/HYPERKERATOSIS,-MINIMAL -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT -ULCERATION,-MODERATE URINARY BLADDER (UB) : >AUTOLYTIC PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,MODERATELY SEVERE CAUSE OF DEATH (CD) : -INFLAMMATION / INFECTION,-PRESENT LN, MANDIBULAR (MN) : -HYPERPLASIA, LYMPHOPLASMACYTIC,MODERATE 2616 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 861 ANIMAL NUMBER: C92787 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/13/99 STUDY DAY OF DEATH: 328 STUDY WEEK OF DEATH: 47 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/13/99 10:30 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -URINARY BLADDER-LARGE LUMEN; 54 X 33 X 30 MM, FILLED WITH A DARK RED FLUID THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), HEART (HT), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), JEJUNUM (JE), RECTUM (RE), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2617 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 862 ANIMAL NUMBER: C92788 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 859.8 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 10:56 PROSECTOR: JOHN S. HALFORD RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -LARGE; ALL LOBES -LIGHT FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE TAN AREAS, PINPOINT AND UP TO 2 X 1 MM; COLLECTED ON ROUTINE SECTIONS HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-MODERATELY SEVERE -GRANULAR CYTOPLASM, EOSINOPHILIC, CENTRILOBULAR,-SLIGHT -NECROSIS, INDIVIDUAL HEPATOCYTE,MINIMAL -VACUOLATION, HEPATOCELLULAR, MIDZONAL / CENTRILOBULAR,-MODERATE -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-SLIGHT -HYPERPLASIA, BILE DUCT,-MODERATE -FIBROSIS, PERIBILIARY,-MINIMAL -B-ADENOMA, HEPATOCELLULAR,- 2 NEOPLASMS PRESENT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL ADRENAL, CORTEX (AC) : -PIGMENT,-SLIGHT 2618 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 863 ANIMAL NUMBER: C92788 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 859.8 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 10:56 PROSECTOR: JOHN S. HALFORD RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY TESTIS (TE) : -LARGE; RIGHT: 27 X 17 X 15 MM (LEFT IS 21 X 15 X 11 MM); RIGHT IS SOFT LUNG (LU) : -LIGHT FOCUS(I)/AREA(S); ALONG CAUDAL MARGIN OF LEFT LOBE: SINGLE TAN AREA, 4 X 3 MM GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN ADRENAL, MEDULLA (MA) : -HYPERPLASIA, MEDULLARY,-SLIGHT TESTIS (TE) : -ATROPHY/DEGENERATION,-SEVERE >NOTE:>UNILATERAL THYMUS (TH) : >SECTION EXAMINED; TISSUE NOT PRESENT LUNG (LU) : >UNREMARKABLE HEART (HT) : -CARDIOMYOPATHY,-SLIGHT THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : -HYPERPLASIA,-MODERATE PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2619 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 864 ANIMAL NUMBER: C92788 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 859.8 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 10:56 PROSECTOR: JOHN S. HALFORD RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAr /i LIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2620 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 865 ANIMAL NUMBER: C92789 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 429.3 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 10:12 PROSECTOR: STEVE VAN ADESTINE RECORDER: KIMBERLY DURLAND POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT' (%) LF ADRENAL (AD0) RT ADRENAL (AD1) BRAIN (BR) LF KIDNEY (KD0) RT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LF TESTIS (TE0) RT TESTIS (TE1) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) .0241 .0290 2.2120 1.9168 1.9639 17.7112 1.7041 .7950 1.5051 1.6074 .0108 .0112 .0056 % .0068 % .5153 % .4465 % .4575 % 4.1256 % .3969 % .1852 % .3506 % .3744 % .0025 % .0026 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0109 .0131 1.0000 .8665 .8878 8.0069 .7704 .3594 .6804 .7267 .0049 .0051 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED THYMUS (TH) : -RED FOCUS(I)/AREA(S); DIFFUSE: MULTIPLE DARK RED PINPOINT FOCI LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-MODERATE -NECROSIS, INDIVIDUAL HEPATOCYTE,MINIMAL -VACUOLATION, HEPATOCELLULAR, MIDZONAL / CENTRILOBULAR,-MINIMAL THYMUS (TH) : >NOT REQUIRED TO BE EXAMINED FOR ANIMAL 2621 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 866 ANIMAL NUMBER: C92789 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 429.3 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 10:12 PROSECTOR: STEVE VAN ADESTINE RECORDER: KIMBERLY DURLAND POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN -MALOCCLUSION; BILATERAL UPPER INCISORS CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAr /i LIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE) 2622 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 867 ANIMAL NUMBER: C92790 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 608.7 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 10:24 PROSECTOR: TODD ACKER RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT' (%) LF ADRENAL (AD0) RT ADRENAL (AD1) BRAIN (BR) LF KIDNEY (KD0) RT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LF TESTIS (TE0) RT TESTIS (TE1) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) .0252 .0215 2.2573 2.6769 2.5370 30.5157 1.8934 .7560 1.5265 1.5153 .0212 .0294 .0041 % .0035 % .3708 % .4398 % .4168 % 5.0133 % .3111 % .1242 % .2508 % .2489 % .0035 % .0048 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0112 .0095 1.0000 1.1859 1.1239 13.5187 .8388 .3349 .6763 .6713 .0094 .0130 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED LIVER (LI) : -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-MODERATELY SEVERE -VACUOLATION, HEPATOCELLULAR, MIDZONAL / CENTRILOBULAR,-SLIGHT KIDNEY (KD) : -PIGMENT, TUBULAR,-MINIMAL -HYALINE DROPLETS, TUBULAR EPITHELIUM,MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT 2623 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 868 ANIMAL NUMBER: C92790 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 608.7 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 10:24 PROSECTOR: TODD ACKER RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL ADRENAL, CORTEX (AC) : -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL THYMUS (TH) : -HEMORRHAGE,-PRESENT -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MINIMAL LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL HEART (HT) : -CARDIOMYOPATHY,-MINIMAL RECTUM (RE) : -PARASITISM,-PRESENT URINARY BLADDER (UB) : -PROTEINACEOUS MATERIAL, LUMINAL,PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2624 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 869 ANIMAL NUMBER: C92790 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 608.7 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 10:24 PROSECTOR: TODD ACKER RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), SKIN (SK), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2625 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 870 ANIMAL NUMBER: C92791 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 826.3 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 10:12 PROSECTOR: JARED FISHEL RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); DORSAL; WEEK 93; 20 X 20MM; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; DORSAL; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -LIGHT FOCUS(I)/AREA(S); DIAPHRAGMATIC SURFACE OF RIGHT MEDIAN LOBE: SINGLE TAN AREA, 3 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MODERATE -DEGENERATION, CYSTIC,-SLIGHT -HYPERPLASIA, BILE DUCT,-SLIGHT -FIBROSIS, PERIBILIARY,-MINIMAL LIVER-OIL RED O (LI0) : -VACUOLES, OIL RED O-POSITIVE, HEPATOCELLULAR,-MODERATE KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MODERATE SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SLIGHT PANCREAS (PA) : -HYPERTROPHY / BASOPHILIA, ACINAR CELL,MINIMAL -INFILTRATION, FAT, INTERSTITIAL,SLIGHT ADRENAL, CORTEX (AC) : -PIGMENT,-MINIMAL 2626 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 871 ANIMAL NUMBER: C92791 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 826.3 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 10:12 PROSECTOR: JARED FISHEL RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY THYROID (TY) : -LARGE; BILATERAL: EACH IS 9 X 4 X 4 MM SKIN (SK) : -MASS(ES); RIGHT LUMBAR REGION: SINGLE MASS, 30 X 25 X 15 MM, RED, TAN, AND FIRM; CUT SURFACE EXUDES A TAN CASEOUS SEMISOLID; COLLECTED; REGIONAL LN (AXILLARY) NORMAL (CAP 22); OVERLYING SKIN HAS A SINGLE DARK BROWN AND MOIST ULCERATED AREA, 15 MM IN DIAMETER; MASS AND OVERLYING SKIN COLLECTED INTACT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MINIMAL HEART (HT) : -CARDIOMYOPATHY,-SLIGHT THYROID (TY) : >UNREMARKABLE PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL STOMACH, GL (ST) : -HYPERPLASIA, EPITHELIAL, GLANDULAR,SLIGHT SKIN (SK) : -B-KERATOACANTHOMA,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2627 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 872 ANIMAL NUMBER: C92791 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 826.3 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 10:12 PROSECTOR: JARED FISHEL RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN), LN, AXILLARY (AX) 2628 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 873 ANIMAL NUMBER: C92792 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 667.3 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 7:42 PROSECTOR: TROY ELLENBECKER RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); AXILLARY REGION-LEFT; WEEK 101; 50 X 60 MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); VENTRAL-ABDOMINAL; WEEK 104; VERIFIED AT NECROPSY: NOT OBSERVED >SKIN & PELAGE; SORE/SCAB; AXILLARY REGION-LEFT; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY KIDNEY (KD) : -LARGE PELVIS(ES); BILATERAL -ROUGH SURFACE, DIFFUSE; BILATERAL ADRENAL, CORTEX (AC) : -DIFFUSELY DARK; BILATERAL: DARK BROWN HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -NECROSIS, INDIVIDUAL HEPATOCYTE,MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL -DEGENERATION, CYSTIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MODERATE -DILATATION, PELVIC,-MODERATE -HYALINE DROPLETS, TUBULAR EPITHELIUM,SEVERE SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MINIMAL PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-SLIGHT ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-MINIMAL -PIGMENT,-MINIMAL BONE, STERNUM (SB) : -X-HEMATOPOIETIC NEOPLASIA (SEE HN),NEOPLASM PRESENT 2629 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 874 ANIMAL NUMBER: C92792 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 667.3 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 7:42 PROSECTOR: TROY ELLENBECKER RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY MUSCLE, SKELETAL (SM) : -MASS(ES); INVOLVING ENTIRE LEFT FORELIMB EXTENDING TO AXILLARY REGION: SINGLE MASS, 60 X 40 X 30 MM, RED, TAN AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (SEE AXILLARY); OVERLYING SKIN HAS A TAN, DRY CRUSTED AREA, 20 X 10 MM; COLLECTED INTACT WITH MASS PITUITARY (PI) : -RED FOCUS(I)/AREA(S); INVOLVING ENTIRE LEFT ASPECT: SINGLE RED AREA, 3 MM IN DIAMETER LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL HEART (HT) : -CARDIOMYOPATHY,-MINIMAL MUSCLE, SKELETAL (SM) : >UNREMARKABLE >NOTE:>SEE SKIN FOR DIAGNOSIS OF MASS TRACHEA (TR) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT ESOPHAGUS (ES) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT THYROID (TY) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT SKIN (SK) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT 2630 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 875 ANIMAL NUMBER: C92792 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 667.3 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 7:42 PROSECTOR: TROY ELLENBECKER RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PROSTATE (PR) : -NODULAR; PROXIMAL SURROUNDING URETHRA: GREEN AND FIRM LN, AXILLARY (AX) : -MOTTLED; BILATERAL: RED AND TAN (CAP 22) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL HEMATO NEOPLASIA (HN) : -M-SARCOMA, HISTIOCYTIC,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, AXILLARY (AX) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT -HEMORRHAGE,-PRESENT 2631 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 876 ANIMAL NUMBER: C92792 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 667.3 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 7:42 PROSECTOR: TROY ELLENBECKER RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), SPINAL CORD (SC), THYMUS (TH), NERVE, SCIATIC (SN), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP) 2632 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 877 ANIMAL NUMBER: C92793 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 311.2 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 11:17 PROSECTOR: JODI THIEREN RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN STATUS LF ADRENAL (AD0) RT ADRENAL (AD1) BRAIN (BR) LF KIDNEY (KD0) RT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LF TESTIS (TE0) RT TESTIS (TE1) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) -------- 12.6977 --------------- -------- 4.0801 % --------------- -------- ---------------------- NOT TAKEN WEIGHT TAKEN NOT TAKEN NOT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS NOTTAK NOTTAK NOTTAK NOTTAK NOTTAK NOTTAK NOTTAK NOTTAK 2633 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 878 ANIMAL NUMBER: C92793 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 311.2 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 11:17 PROSECTOR: JODI THIEREN RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) 2634 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 879 ANIMAL NUMBER: C92794 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 05/26/99 STUDY DAY OF DEATH: 402 STUDY WEEK OF DEATH: 58 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 05/26/99 10:55 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >EYES; RED DISCHARGE; EYE-RIGHT; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; BROWN HAIR COAT; VENTRAL-ABDOMINAL; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: NOT OBSERVED OBSERVATIONS NECROPSY HISTOPATHOLOGY LIVER (LI) : -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-MODERATE -VACUOLATION, HEPATOCELLULAR, MIDZONAL / CENTRILOBULAR,-SLIGHT KIDNEY (KD) : -MINERALIZATION, TUBULAR,-MODERATE -INFLAMMATION, CHRONIC-ACTIVE, PELVIC,SLIGHT -NECROSIS, PAPILLARY,-MODERATELY SEVERE SPLEEN (SP) : -DEPLETION, LYMPHOCYTIC,-MODERATELY SEVERE PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,MODERATELY SEVERE LN, MESENTERIC (MS) : -MINERALIZATION, VASCULAR,-SLIGHT MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,-MODERATE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATELY SEVERE 2635 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 880 ANIMAL NUMBER: C92794 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 05/26/99 STUDY DAY OF DEATH: 402 STUDY WEEK OF DEATH: 58 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 05/26/99 10:55 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -DISCHARGE-OCULAR; RIGHT: DRY, RED MATERIAL LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL HEART (HT) : -CARDIOMYOPATHY,-SLIGHT -MINERALIZATION, VASCULAR,-MODERATE -MINERALIZATION,-MODERATE PITUITARY (PI) : -HYPERPLASIA,-MINIMAL SALIV GL, MANDIB (SG) : -ATROPHY,-MODERATE STOMACH, GL (ST) : -MINERALIZATION, GLANDULAR MUCOSA,MODERATE ILEUM (IL) : >AUTOLYTIC URINARY BLADDER (UB) : -HYPERPLASIA, TRANSITIONAL CELL,-SLIGHT -INFILTRATE, MACROPHAGE, PIGMENTED,SLIGHT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT CAUSE OF DEATH (CD) : -INFECTION / NECROSIS / CALCULUS, URINARY TRACT,-PRESENT 2636 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 881 ANIMAL NUMBER: C92794 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 05/26/99 STUDY DAY OF DEATH: 402 STUDY WEEK OF DEATH: 58 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 05/26/99 10:55 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; BOTH REGIONS, EXTENDING TO VENTRAL ABDOMINAL REGION: MOIST, BROWN MATERIAL 2637 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 882 ANIMAL NUMBER: C92794 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 05/26/99 STUDY DAY OF DEATH: 402 STUDY WEEK OF DEATH: 58 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 05/26/99 10:55 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) (TR), 2638 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 883 ANIMAL NUMBER: C92795 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 676.3 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 9:00 PROSECTOR: JOHN S. HALFORD RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT' (%) LF ADRENAL (AD0) RT ADRENAL (AD1) BRAIN (BR) LF KIDNEY (KD0) RT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LF TESTIS (TE0) RT TESTIS (TE1) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) .0216 .0266 2.4060 2.1778 2.2393 29.6021 1.9817 .7006 2.0765 2.0178 .0205 .0277 .0032 % .0039 % .3558 % .3220 % .3311 % 4.3771 % .2930 % .1036 % .3070 % .2984 % .0030 % .0041 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0090 .0111 1.0000 .9052 .9307 12.3034 .8236 .2912 .8631 .8387 .0085 .0115 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-MODERATELY SEVERE -NECROSIS, INDIVIDUAL HEPATOCYTE,MINIMAL -VACUOLATION, HEPATOCELLULAR, MIDZONAL / CENTRILOBULAR,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL -VACUOLATION, HEPATOCELLULAR,-MINIMAL 2639 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 884 ANIMAL NUMBER: C92795 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 676.3 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 9:00 PROSECTOR: JOHN S. HALFORD RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT ADRENAL, CORTEX (AC) : -VACUOLATION, DIFFUSE,-SLIGHT THYMUS (TH) : -M-THYMIC CARCINOMA,-NEOPLASM PRESENT LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL HEART (HT) : -CARDIOMYOPATHY,-MINIMAL -HEMORRHAGE,-MINIMAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2640 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 885 ANIMAL NUMBER: C92795 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 676.3 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 9:00 PROSECTOR: JOHN S. HALFORD RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2641 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 886 ANIMAL NUMBER: C92796 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 543.3 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 10:00 PROSECTOR: TODD ACKER RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT' (%) LF ADRENAL (AD0) RT ADRENAL (AD1) BRAIN (BR) LF KIDNEY (KD0) RT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LF TESTIS (TE0) RT TESTIS (TE1) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) .0276 .0283 2.4368 2.1147 2.1334 20.3375 1.9590 .8078 1.5539 1.6589 .0163 .0156 .0051 % .0052 % .4485 % .3892 % .3927 % 3.7433 % .3606 % .1487 % .2860 % .3053 % .0030 % .0029 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0113 .0116 1.0000 .8678 .8755 8.3460 .8039 .3315 .6377 .6808 .0067 .0064 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED LIVER (LI) : -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-MODERATE -VACUOLATION, HEPATOCELLULAR,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL 2642 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 887 ANIMAL NUMBER: C92796 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 543.3 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 10:00 PROSECTOR: TODD ACKER RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY THYMUS (TH) : -LARGE; 16 X 12 X 4 MM -GELATINOUS; DIFFUSE: DARK RED GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN ADRENAL, CORTEX (AC) : -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL THYMUS (TH) : >SECTION EXAMINED; TISSUE NOT PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2643 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 888 ANIMAL NUMBER: C92796 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 543.3 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 10:00 PROSECTOR: TODD ACKER RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAr /i LIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2644 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 889 ANIMAL NUMBER: C92797 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/01/00 STUDY DAY OF DEATH: 653 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: 1010.8 GRAMS DATE AND TIME OF NECROPSY: 02/01/00 14:37 PROSECTOR: GREGORY CODDINGTON RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); VENTRAL-ABDOMINAL-RIGHT; WEEK 81; 120 X 110MM; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; LIMITED USE; LIMB-HINDRIGHT; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY LIVER (LI) : -DIFFUSELY DARK; ALL LOBES: BROWN -CYST(S); ALL LOBES: MULTIPLE CLEAR FLUID FILLED CYSTS, PINPOINT AND UP TO 1 MM IN DIAMETER; COLLECTED ON ROUTINE SECTIONS HISTOPATHOLOGY BRAIN (BR) : -MINERALIZATION,-MINIMAL LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -DEGENERATION, CYSTIC,-SLIGHT -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL -FIBROSIS, PERIBILIARY,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATELY SEVERE PANCREAS (PA) : -ATROPHY, ACINAR,-MINIMAL ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-SLIGHT -HYPERTROPHY, CORTICAL CELL,-SLIGHT 2645 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 890 ANIMAL NUMBER: C92797 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/01/00 STUDY DAY OF DEATH: 653 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: 1010.8 GRAMS DATE AND TIME OF NECROPSY: 02/01/00 14:37 PROSECTOR: GREGORY CODDINGTON RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY TESTIS (TE) : -RED/YELLOW FOCUS(I)/AREA(S); LEFT, BENEATH TUNICA: MULTIPLE RED AND YELLOW AREAS, UP TO 6 X 5 MM THYROID (TY) : -LARGE; LEFT: 10 X 7 X 6 MM (RIGHT IS 8 X 4 X 3 MM) TESTIS (TE) : -B-INTERSTITIAL CELL TUMOR,-NEOPLASM PRESENT MARROW, FEMUR (FM) : -HYPERPLASIA, ERYTHROID, MARROW,MODERATE MARROW, STERNUM (SE) : -HYPERPLASIA, ERYTHROID, MARROW,MODERATE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATELY SEVERE LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL HEART (HT) : -CARDIOMYOPATHY,-MINIMAL THYROID (TY) : -M-CARCINOMA, FOLLICULAR CELL,-NEOPLASM PRESENT PITUITARY (PI) : -HYPERPLASIA,-MODERATE PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL EPIDIDYMIDES (EP) : -IMMATURE SPERM FORMS,-SLIGHT CAUSE OF DEATH (CD) : -NEOPLASM, MAMMARY GLAND,-PRESENT 2646 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 891 ANIMAL NUMBER: C92797 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/01/00 STUDY DAY OF DEATH: 653 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: 1010.8 GRAMS DATE AND TIME OF NECROPSY: 02/01/00 14:37 PROSECTOR: GREGORY CODDINGTON RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY MAMMARY, MALE (MM) : -MASS(ES); LEFT AXILLARY REGION: SINGLE MASS, 20 X 15 X 8 MM, TAN, LOBULATED, AND SOFT; SAME ON CUT SURFACE (CAP 22); REGIONAL LN (AXILLARY) NORMAL (CAP 23) -MASS #2; RIGHT INGUINAL REGION, EXTENDING TO RIGHT LATERAL ABDOMINAL REGION: SINGLE MASS, 135 X 115 X 55 MM (410.3 GRAMS), RED, DARK RED, TAN, GREY, LOBULATED, AND FIRM; SAME ON CUT SURFACE; PORTION COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN MAMMARY, MALE (MM) : -B-FIBROADENOMA,-NEOPLASM PRESENT 2647 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 892 ANIMAL NUMBER: C92797 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/01/00 STUDY DAY OF DEATH: 653 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: 1010.8 GRAMS DATE AND TIME OF NECROPSY: 02/01/00 14:37 PROSECTOR: GREGORY CODDINGTON RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUm /s KULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), HEMATO NEOPLASIA (HN), LN, AXILLARY (AX) 2648 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 893 ANIMAL NUMBER: C92798 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 301.4 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 11:00 PROSECTOR: LYNN HORNER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN STATUS LF ADRENAL (AD0) RT ADRENAL (AD1) BRAIN (BR) LF KIDNEY (KD0) RT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LF TESTIS (TE0) RT TESTIS (TE1) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) -------- 13.1989 --------------- -------- 4.3792 % --------------- -------- ---------------------- NOT TAKEN WEIGHT TAKEN NOT TAKEN NOT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS NOTTAK NOTTAK NOTTAK NOTTAK NOTTAK NOTTAK NOTTAK NOTTAK 2649 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 894 ANIMAL NUMBER: C92798 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 301.4 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 11:00 PROSECTOR: LYNN HORNER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) 2650 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 895 ANIMAL NUMBER: C92799 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 512.6 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 10:07 PROSECTOR: TROY ELLENBECKER RECORDER: KIMBERLY DURLAND POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT' (%) LF ADRENAL (AD0) RT ADRENAL (AD1) BRAIN (BR) LF KIDNEY (KD0) RT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LF TESTIS (TE0) RT TESTIS (TE1) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) .0288 .0286 2.4425 2.0511 2.0596 23.1666 1.9190 .8479 1.7019 1.7440 .0094 .0104 .0056 % .0056 % .4765 % .4001 % .4018 % 4.5194 % .3744 % .1654 % .3320 % .3402 % ..00002108 % % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0118 .0117 1.0000 .8398 .8432 9.4848 .7857 .3471 .6968 .7140 .0038 .0043 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-SLIGHT -VACUOLATION, HEPATOCELLULAR, MIDZONAL / CENTRILOBULAR,-SLIGHT KIDNEY (KD) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-MINIMAL -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL 2651 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 896 ANIMAL NUMBER: C92799 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 512.6 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 10:07 PROSECTOR: TROY ELLENBECKER RECORDER: KIMBERLY DURLAND POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE) 2652 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 897 ANIMAL NUMBER: C92800 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 601.5 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 10:27 PROSECTOR: GREGORY CODDINGTON RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -LIGHT FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE TAN AREAS, UP TO 3 MM IN DIAMETER; COLLECTED ON ROUTINE SECTIONS PANCREAS (PA) : -MASS(ES); INVOLVING ENTIRE ORGAN: SINGLE MASS, 27 X 18 X 14 MM, RED, DARK RED, TAN, GREY, LOBULATED AND FIRM; SOME SECTIONS EXUDE A DARK RED FLUID; (CAP 22); REGIONAL LN (PANCREATIC) NOT IDENTIFIED HISTOPATHOLOGY BRAIN (BR) : -MINERALIZATION,-MINIMAL LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MODERATE -DEGENERATION, CYSTIC,-MINIMAL -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL -FIBROSIS, PERIBILIARY,-MINIMAL -B-ADENOMA, HEPATOCELLULAR,- 2 NEOPLASMS PRESENT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SLIGHT PANCREAS (PA) : -M-CARCINOMA, ISLET CELL,-NEOPLASM PRESENT -INFLAMMATION, VASCULAR,-MODERATE 2653 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 898 ANIMAL NUMBER: C92800 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 601.5 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 10:27 PROSECTOR: GREGORY CODDINGTON RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -LARGE; 5 X 4 X 3 MM; APPEARS SWOLLEN STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: MULTIPLE BLACK AREAS, UP TO 2 X 1 MM GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-MINIMAL -HYPERTROPHY, CORTICAL CELL,-MINIMAL -PIGMENT,-MINIMAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),SLIGHT LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL HEART (HT) : -CARDIOMYOPATHY,-MINIMAL MUSCLE, SKELETAL (SM) : -ATROPHY,-MODERATELY SEVERE NERVE, SCIATIC (SN) : -DEGENERATION,-SLIGHT THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : -EROSION,-MINIMAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2654 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 899 ANIMAL NUMBER: C92800 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 601.5 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 10:27 PROSECTOR: GREGORY CODDINGTON RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2655 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 900 ANIMAL NUMBER: C92801 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 05/17/99 STUDY DAY OF DEATH: 393 STUDY WEEK OF DEATH: 57 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 05/17/99 13:49 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); VENTRAL-ABDOMINAL-RIGHT; WEEK 53; 15 X 15MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK 56; 70 X 80MM; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -LARGE; ALL LOBES: THICKENED -DIFFUSELY LIGHT; ALL LOBES: TAN KIDNEY (KD) : -LARGE PELVIS(ES); LEFT -LARGE; LEFT: 30 X 20 X 15 MM (FOR COMPARISON, RIGHT IS 25 X 15 X 10 MM) TESTIS (TE) : -SMALL; LEFT: 2 0 X 1 0 X 8 MM (FOR COMPARISON, RIGHT IS 25 X 15 X 10 MM) HISTOPATHOLOGY LIVER (LI) : -GRANULAR CYTOPLASM, EOSINOPHILIC, CENTRILOBULAR,-MINIMAL -PIGMENT, HEPATOCELLULAR, CENTRILOBULAR,-MINIMAL -NECROSIS, COAGULATIVE,-MODERATE -DEGENERATION / NECROSIS, CENTRILOBULAR,-SLIGHT -LEUKOCYTOSIS, SINUSOIDAL,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SLIGHT -DILATATION, PELVIC,-MODERATE PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL ADRENAL, CORTEX (AC) : -HYPERTROPHY, CORTICAL CELL,-SLIGHT TESTIS (TE) : -ATROPHY/DEGENERATION,-MINIMAL MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,-MINIMAL MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,-MODERATE 2656 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 901 ANIMAL NUMBER: C92801 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 05/17/99 STUDY DAY OF DEATH: 393 STUDY WEEK OF DEATH: 57 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 05/17/99 13:49 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY SEMINAL VESICLES (SV) : -GELATINOUS; RIGHT: CLEAR LN, MEDIASTINAL (ML) : -LARGE; UP TO 5 X 3 X 2 MM (CAP 22) -DIFFUSELY RED; DARK RED CAVITY, ABDOM (PC) : -MASS(ES); WITHIN ABDOMINAL CAVITY, JUST CAUDAL TO LEFT KIDNEY: SINGLE MASS, 100 X 80 X 60 MM (135.7 GRAMS), RED, DARK RED, TAN, FIRM, AND LOBULATED; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (RENAL) NOT IDENTIFIED; POSSIBLY CONSISTENT WITH MASS (VENTRAL HIND LEFT) NOTED IN CLINICAL OBSERVATIONS THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATELY SEVERE JEJUNUM (JE) : >AUTOLYTIC PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT SEMINAL VESICLES (SV) : -SECRETION, INCREASED,-PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, ABDOMINAL CAVITY,-PRESENT LN, MEDIASTINAL (ML) : -HEMORRHAGE,-PRESENT CAVITY, ABDOM (PC) : -M-FIBROSARCOMA,-NEOPLASM PRESENT PREPUTIAL GLAND (PG) : -INFLAMMATION, CHRONIC-ACTIVE,-SEVERE 2657 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 902 ANIMAL NUMBER: C92801 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 05/17/99 STUDY DAY OF DEATH: 393 STUDY WEEK OF DEATH: 57 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 05/17/99 13:49 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PREPUTIAL GLAND (PG) : -LARGE; RIGHT: 40 X 30 X 15 MM (FOR COMPARISON, LEFT IS 14 X 3 X 2 MM); RIGHT IS FILLED WITH GREEN, CREAMY SEMIFLUID (CAP 21); POSSIBLY CONSISTENT WITH MASS (VENTRAL HIND RIGHT) NOTED IN CLINICAL OBSERVATIONS GENERAL COMMENT (GC) : -FLUID-ABDOMINAL CAVITY; DARK RED SEMIFLUID -PALE MUCOUS MEMBRANES 2658 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 903 ANIMAL NUMBER: C92801 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 05/17/99 STUDY DAY OF DEATH: 393 STUDY WEEK OF DEATH: 57 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 05/17/99 13:49 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), EPIDIDYMIDES (EP), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), ILEUM (IL), CECUM (CE), COLON RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) (CO), 2659 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 904 ANIMAL NUMBER: C92802 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 949.1 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 8:43 PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-MODERATELY SEVERE -GRANULAR CYTOPLASM, EOSINOPHILIC, CENTRILOBULAR,-MODERATE -VACUOLATION, HEPATOCELLULAR, MIDZONAL / CENTRILOBULAR,-MODERATELY SEVERE -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MODERATE -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-SLIGHT -INFILTRATE, MACROPHAGE, PIGMENTED,SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MINIMAL ADRENAL, CORTEX (AC) : -PIGMENT,-MINIMAL ADRENAL, MEDULLA (MA) : -HYPERPLASIA, MEDULLARY,-SLIGHT 2660 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 905 ANIMAL NUMBER: C92802 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 949.1 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 8:43 PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY STOMACH, GL (ST) : -THICKENED WALL; DIFFUSE GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -ANIMAL OBESE GENERAL INFORMATION (XX) : >NOTE:>ANIMAL IDENTIFICATION TRANSPONDER DAMAGED AT NECROPSY TESTIS (TE) : -HYPERPLASIA, INTERSTITIAL CELL (LEYDIG CELL),-MINIMAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATELY SEVERE HEART (HT) : -CARDIOMYOPATHY,-MINIMAL THYROID (TY) : -DILATATION / CYST, FOLLICULAR,-PRESENT STOMACH, GL (ST) : >UNREMARKABLE PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT SEMINAL VESICLES (SV) : -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2661 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 906 ANIMAL NUMBER: C92802 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 949.1 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 8:43 PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2662 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 907 ANIMAL NUMBER: C92803 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 703.7 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 10:43 PROSECTOR: TROY ELLENBECKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -LARGE; ALL LOBES, APPEAR ROUNDED -LIGHT FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE TAN AREAS, UP TO 3 MM IN DIAMETER, COLLECTED ON ROUTINE SECTIONS HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-MODERATELY SEVERE -GRANULAR CYTOPLASM, EOSINOPHILIC, CENTRILOBULAR,-MODERATE -VACUOLATION, HEPATOCELLULAR, MIDZONAL / CENTRILOBULAR,-MODERATE -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MODERATE -ANGIECTASIS,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL PANCREAS (PA) : -INFILTRATION, FAT, INTERSTITIAL,MODERATE ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-SLIGHT -HYPERTROPHY, CORTICAL CELL,-SLIGHT -HYPERPLASIA, CORTICAL,-MINIMAL TESTIS (TE) : -MINERALIZATION, VASCULAR,-MINIMAL 2663 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 908 ANIMAL NUMBER: C92803 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 703.7 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 10:43 PROSECTOR: TROY ELLENBECKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: SINGLE DARK BROWN AREA, 2 X 1 MM GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN MARROW, FEMUR (FM) : -HYPOCELLULAR, MARROW,-MODERATE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),SLIGHT HEART (HT) : -CARDIOMYOPATHY,-MINIMAL THYROID (TY) : -DILATATION / CYST, FOLLICULAR,-PRESENT PITUITARY (PI) : -HYPERPLASIA,-MINIMAL STOMACH, GL (ST) : >UNREMARKABLE PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2664 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 909 ANIMAL NUMBER: C92803 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 703.7 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 10:43 PROSECTOR: TROY ELLENBECKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2665 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 910 ANIMAL NUMBER: C92804 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 928.4 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 7:44 PROSECTOR: JARED FISHEL RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) -MOTTLED; ALL LOBES: RED AND TAN HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-MODERATELY SEVERE -VACUOLATION, HEPATOCELLULAR, MIDZONAL / CENTRILOBULAR,-MODERATELY SEVERE -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-SLIGHT -DEGENERATION, CYSTIC,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-MODERATE -HYPERTROPHY / BASOPHILIA, ACINAR CELL,MINIMAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATE LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL 2666 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 911 ANIMAL NUMBER: C92804 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 928.4 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 7:44 PROSECTOR: JARED FISHEL RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY THYROID (TY) : -LARGE; BILATERAL: EACH IS 9 X 7 X 4 MM GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -ANIMAL OBESE LUNG (LU) : -HYPERPLASIA, TYPE II PNEUMOCYTE,MINIMAL HEART (HT) : -CARDIOMYOPATHY,-SLIGHT THYROID (TY) : >UNREMARKABLE PITUITARY (PI) : -HYPERPLASIA,-MODERATE CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2667 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 912 ANIMAL NUMBER: C92804 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 928.4 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 7:44 PROSECTOR: JARED FISHEL RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2668 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 913 ANIMAL NUMBER: C92805 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/01/99 STUDY DAY OF DEATH: 500 STUDY WEEK OF DEATH: 72 TERMINAL BODY WEIGHT: 555.8 GRAMS DATE AND TIME OF NECROPSY: 09/01/99 15:24 PROSECTOR: JERALD W. JOHNSON RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; SORE/SCAB; PAW-HINDLEFT; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: NOT OBSERVED >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >EYES; RED DISCHARGE; EYE-LEFT; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) HISTOPATHOLOGY BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -NECROSIS, INDIVIDUAL HEPATOCYTE,MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL -FIBROSIS, PERIBILIARY,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, PERIPORTAL,-MINIMAL -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, PELVIC,-MINIMAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MINIMAL 2669 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 914 ANIMAL NUMBER: C92805 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/01/99 STUDY DAY OF DEATH: 500 STUDY WEEK OF DEATH: 72 TERMINAL BODY WEIGHT: 555.8 GRAMS DATE AND TIME OF NECROPSY: 09/01/99 15:24 PROSECTOR: JERALD W. JOHNSON RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 12 X 9 X 7 MM, DARK RED, SOFT SEMINAL VESICLES (SV) : -SMALL; BILATERAL: EACH IS 14 X 8 X 3 MM FOOT/FOOTPAD (FP) : -CRUSTED AREA(S); LEFT HIND FOOTPAD: SINGLE DARK BROWN, TAN, DRY CRUSTED AREA, 8 X 6 MM; COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; LEFT: DARK RED, DRY MATERIAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATE THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,MODERATELY SEVERE SEMINAL VESICLES (SV) : -ATROPHY,-MODERATELY SEVERE CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT FOOT/FOOTPAD (FP) : -INFLAMMATION, CHRONIC-ACTIVE,MODERATELY SEVERE -ULCERATION,-MODERATE -ACANTHOSIS / HYPERKERATOSIS,MODERATELY SEVERE 2670 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 915 ANIMAL NUMBER: C92805 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/01/99 STUDY DAY OF DEATH: 500 STUDY WEEK OF DEATH: 72 TERMINAL BODY WEIGHT: 555.8 GRAMS DATE AND TIME OF NECROPSY: 09/01/99 15:24 PROSECTOR: JERALD W. JOHNSON RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2671 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 916 ANIMAL NUMBER: C92806 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/10/00 STUDY DAY OF DEATH: 691 STUDY WEEK OF DEATH: 99 TERMINAL BODY WEIGHT: 515.7 GRAMS DATE AND TIME OF NECROPSY: 03/10/00 13:37 PROSECTOR: TODD ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: NOT OBSERVED >APPEARANCE; MISSING; TEETH; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; VENTRALABDOMINAL-RIGHT; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; NO FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >RESPIRATION; LABORED; VERIFIED AT NECROPSY: NOT APPLICABLE >RESPIRATION; IRREGULAR; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; THIN; VERIFIED AT NECROPSY: NOT OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -MOTTLED; ALL LOBES: RED AND BROWN HISTOPATHOLOGY EYE (EY) : -DYSTROPHY, CORNEAL IN EARLY STAGES, CONSISTS PRIMARILY OF FOCAL MINERALIZATION,-MINIMAL BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-MODERATE KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-SEVERE ADRENAL, CORTEX (AC) : -PIGMENT,-SLIGHT MARROW, STERNUM (SE) : -HYPERPLASIA, ERYTHROID, MARROW,MINIMAL 2672 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 917 ANIMAL NUMBER: C92806 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/10/00 STUDY DAY OF DEATH: 691 STUDY WEEK OF DEATH: 99 TERMINAL BODY WEIGHT: 515.7 GRAMS DATE AND TIME OF NECROPSY: 03/10/00 13:37 PROSECTOR: TODD ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 9 X 7 X 6 MM, DARK RED, TAN, AND SOFT STOMACH, GL (ST) : -THICKENED WALL; MUCOSA: DIFFUSE STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA: MULTIPLE TAN ULCERATED AREAS, UP 1 MM IN DIAMETER SKIN (SK) : -CRUSTED AREA(S); RIGHT VENTRAL ABDOMINAL REGION: SINGLE DARK RED DRY AREA, 5 X 3 MM (CAP 22) THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATELY SEVERE LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL HEART (HT) : -CARDIOMYOPATHY,-SLIGHT MUSCLE, SKELETAL (SM) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : >UNREMARKABLE STOMACH, NONGL (SU) : -ACANTHOSIS / HYPERKERATOSIS,-MODERATE -EDEMA,-PRESENT -ULCERATION,-MINIMAL -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE SKIN (SK) : -ACANTHOSIS/HYPERKERATOSIS,-SLIGHT -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE -BACTERIAL COLONIES,-PRESENT -SUPERFICIAL CRUSTING,-MODERATELY SEVERE 2673 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 918 ANIMAL NUMBER: C92806 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/10/00 STUDY DAY OF DEATH: 691 STUDY WEEK OF DEATH: 99 TERMINAL BODY WEIGHT: 515.7 GRAMS DATE AND TIME OF NECROPSY: 03/10/00 13:37 PROSECTOR: TODD ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PROSTATE (PR) : -NODULAR; PROXIMAL SURROUNDING URETHRA: GREEN AND FIRM GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -TEETH MISSING/BROKEN/WORN; LEFT UPPER INCISOR: MISSING RIGHT UPPER INCISOR: BROKEN AT BASE -GI TRACT-LARGE LUMEN; CECUM: UP 20 MM IN DIAMETER URINARY BLADDER (UB) : -PROTEINACEOUS MATERIAL, LUMINAL,PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT MAMMARY, MALE (MM) : -GALACTOCELE,-PRESENT 2674 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 919 ANIMAL NUMBER: C92806 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/10/00 STUDY DAY OF DEATH: 691 STUDY WEEK OF DEATH: 99 TERMINAL BODY WEIGHT: 515.7 GRAMS DATE AND TIME OF NECROPSY: 03/10/00 13:37 PROSECTOR: TODD ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAr /i LIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), BONE,STERNUM (SB), SPINAL CORD (SC), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY),PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2675 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 920 ANIMAL NUMBER: C92807 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/07/99 STUDY DAY OF DEATH: 597 STUDY WEEK OF DEATH: 86 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/07/99 8:28 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) HISTOPATHOLOGY BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-SLIGHT -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-MODERATE -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-SLIGHT -DEGENERATION, CYSTIC,-MINIMAL -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, PELVIC,-SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL -B-ADENOMA, ISLET CELL,-NEOPLASM PRESENT 2676 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 921 ANIMAL NUMBER: C92807 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/07/99 STUDY DAY OF DEATH: 597 STUDY WEEK OF DEATH: 86 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/07/99 8:28 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 8 X 6 X 5 MM, RED, DARK RED, TAN AND SOFT ADRENAL, CORTEX (AC) : -PIGMENT,-MINIMAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATE LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL HEART (HT) : -CARDIOMYOPATHY,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 2677 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 922 ANIMAL NUMBER: C92807 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/07/99 STUDY DAY OF DEATH: 597 STUDY WEEK OF DEATH: 86 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/07/99 8:28 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG), GENERAL COMMENT (GC) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2678 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 923 ANIMAL NUMBER: C92808 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 599.6 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 9:44 PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK 98; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); INGUINAL REGION-LEFT; WEEK 98; 10 X 10 MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); AXILLARY REGION-LEFT; WEEK 103; 10 X 10 MM; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; AXILLARY REGION-LEFT; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-SLIGHT -GRANULAR CYTOPLASM, EOSINOPHILIC, CENTRILOBULAR,-MINIMAL -VACUOLATION, HEPATOCELLULAR, MIDZONAL / CENTRILOBULAR,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE PANCREAS (PA) : -ATROPHY, ACINAR,-SEVERE ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-MODERATELY SEVERE -PIGMENT,-MINIMAL 2679 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 924 ANIMAL NUMBER: C92808 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 599.6 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 9:44 PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -RAISED AREA(S); LEFT POSTERIOR ASPECT: SINGLE DARK RED, SOFT AREA, X 3 X 2 MM STOMACH, GL (ST) : -THICKENED WALL; DIFFUSE 4 SKIN (SK) : -MASS(ES); LEFT AXILLARY REGION: SINGLE MASS, 12 X 8 X 8 MM, TAN AND FIRM; SAME ON CUT SURFACE; OVERLYING SKIN HAS A TAN AND DRY ULCERATED AREA, 5 X 4 MM; COLLECTED INTACT WITH MASS; REGIONAL LN (AXILLARY) NORMAL (CAP 27) THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MINIMAL HEART (HT) : -CARDIOMYOPATHY,-SLIGHT THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : -HYPERPLASIA, EPITHELIAL, GLANDULAR,SLIGHT SKIN (SK) : -CYST, EPIDERMAL INCLUSION,-PRESENT -B-KERATOACANTHOMA,-NEOPLASM PRESENT URINARY BLADDER (UB) : -PROTEINACEOUS MATERIAL, LUMINAL,PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2680 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 925 ANIMAL NUMBER: C92808 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 599.6 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 9:44 PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL: DARK RED (CAP 23) MAMMARY, MALE (MM) : -CYST(S); BILATERAL PECTORAL REGIONS: MULTIPLE TAN CREAMY FLUID FILLED CYSTS, UP TO 8 MM IN DIAMETER (CAP 22) -MASS(ES); LEFT VENTRAL ABDOMINAL REGION: SINGLE MASS, 15 X 11 X 8 MM, TAN, RED AND FIRM; SAME ON CUT SURFACE; (CAP 24); REGIONAL LN (INGUINAL) NOT IDENTIFIED -MASS #2; LEFT INGUINAL REGION: SINGLE MASS, 14 X 10 X 8 MM, TAN AND FIRM; CUT SURFACE EXUDES A BROWN CREAMY SEMIFLUID; (CAP 25); REGIONAL LN (INGUINAL) NOT IDENTIFIED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN LN, AXILLARY (AX) : -HYPERPLASIA, LYMPHOPLASMACYTIC,MODERATE LN, MANDIBULAR (MN) : -HYPERPLASIA, LYMPHOPLASMACYTIC,MODERATE -HEMORRHAGE,-PRESENT MAMMARY, MALE (MM) : -GALACTOCELE,-PRESENT -B-ADENOMA,-NEOPLASM PRESENT >NOTE:>MASS #2 IS THE ADENOMA MASS #1 IS AN EPIDERMAL INCLUSION CYST DIAGNOSED UNDER SKIN 2681 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 926 ANIMAL NUMBER: C92808 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 599.6 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 9:44 PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) (SM), (SG), 2682 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 927 ANIMAL NUMBER: C92809 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 814.4 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 11:37 PROSECTOR: TODD ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); AXILLARY REGION-LEFT; WEEK 85; 70 X 80 MM; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-SLIGHT -PIGMENT, HEPATOCELLULAR, CENTRILOBULAR,-SLIGHT -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-SLIGHT -DEGENERATION, CYSTIC,-SLIGHT -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MODERATELY SEVERE SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATELY SEVERE PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,MODERATELY SEVERE -B-ADENOMA, ISLET CELL,-NEOPLASM PRESENT 2683 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 928 ANIMAL NUMBER: C92809 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 814.4 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 11:37 PROSECTOR: TODD ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY LN, MESENTERIC (MS) : -MASS(ES); WITHIN CHAIN: SINGLE MASS, 6 MM IN DIAMETER, DARK RED, TAN, AND SOFT; SAME ON CUT SURFACE; COLLECTED INTACT PITUITARY (PI) : -MASS(ES); 8 X 6 X 6 MM, DARK RED, GRAY, SOFT STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: MULTIPLE BLACK AREAS, UP TO 2 X 1 MM -THICKENED WALL; MUCOSA: DIFFUSE ADRENAL, CORTEX (AC) : -HYPERTROPHY, CORTICAL CELL,-MINIMAL LN, MESENTERIC (MS) : -HYPERPLASIA, LYMPHOPLASMACYTIC,MODERATE >NOTE:>SEE PANCREAS FOR DIAGNOSIS OF MASS THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATELY SEVERE LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL HEART (HT) : -CARDIOMYOPATHY,-SLIGHT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : -EROSION,-MINIMAL -HYPERPLASIA, EPITHELIAL, GLANDULAR,SLIGHT SKIN (SK) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT -B-LIPOMA,-NEOPLASM PRESENT URINARY BLADDER (UB) : -PROTEINACEOUS MATERIAL, LUMINAL,PRESENT 2684 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 929 ANIMAL NUMBER: C92809 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 814.4 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 11:37 PROSECTOR: TODD ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY MAMMARY, MALE (MM) : -MASS(ES); LEFT AXILLARY REGION: SINGLE MASS, 80 X 80 X 25 MM, TAN AND SOFT; SAME ON CUT SURFACE; PORTION COLLECTED; REGIONAL LN (AXILLARY) NORMAL (CAP 22) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT MAMMARY, MALE (MM) : >NOT REQUIRED TO BE EXAMINED FOR ANIMAL >NOTE:>SEE SKIN FOR DIAGNOSIS OF MASS 2685 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 930 ANIMAL NUMBER: C92809 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 814.4 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 11:37 PROSECTOR: TODD ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN), LN, AXILLARY (AX) 2686 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 931 ANIMAL NUMBER: C92810 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/21/00 STUDY DAY OF DEATH: 673 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 419.9 GRAMS DATE AND TIME OF NECROPSY: 02/21/00 14:45 PROSECTOR: JERALD W. JOHNSON RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; THIN; VERIFIED AT NECROPSY: NOT OBSERVED >EYES; SQUINTED; PERI-ORBITAL; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -MOTTLED; ALL LOBES: TAN AND BROWN SPLEEN (SP) : -SMALL; 27 X 6 X 4 MM HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-SLIGHT -GRANULAR CYTOPLASM, EOSINOPHILIC, CENTRILOBULAR,-MINIMAL -PIGMENT, HEPATOCELLULAR, CENTRILOBULAR,-SLIGHT -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, PELVIC,-SLIGHT -MINERALIZATION, TUBULAR,-MINIMAL -VACUOLATION, TUBULAR EPITHELIUM,SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATELY SEVERE PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,MODERATELY SEVERE 2687 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 932 ANIMAL NUMBER: C92810 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/21/00 STUDY DAY OF DEATH: 673 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 419.9 GRAMS DATE AND TIME OF NECROPSY: 02/21/00 14:45 PROSECTOR: JERALD W. JOHNSON RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY TESTIS (TE) : -CONTAINS FLUID; LEFT: RED FLUID -DIFFUSELY RED; LEFT: DARK RED -LARGE; LEFT: 30 X 17 X 15 MM (RIGHT IS 22 X 13 X 12 MM) -MOTTLED; RIGHT: RED AND TAN PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL ADRENAL, CORTEX (AC) : -PIGMENT,-MINIMAL ADRENAL, MEDULLA (MA) : -UNILATERALLY EXAMINED,-PRESENT TESTIS (TE) : >UNREMARKABLE MARROW, FEMUR (FM) : -HYPOCELLULAR, MARROW,-MODERATELY SEVERE MARROW, STERNUM (SE) : -HYPOCELLULAR, MARROW,-MODERATELY SEVERE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),SLIGHT LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL HEART (HT) : -CARDIOMYOPATHY,-SLIGHT 2688 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 933 ANIMAL NUMBER: C92810 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/21/00 STUDY DAY OF DEATH: 673 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 419.9 GRAMS DATE AND TIME OF NECROPSY: 02/21/00 14:45 PROSECTOR: JERALD W. JOHNSON RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 10 MM IN DIAMETER, DARK RED, TAN, AND SOFT STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA: MULTIPLE TAN ERODED AREAS, UP TO 2 MM IN DIAMETER -THICKENED WALL; DIFFUSE PROSTATE (PR) : -NODULAR; PROXIMAL, SURROUNDING URETHRA: TAN, GREEN, AND FIRM SEMINAL VESICLES (SV) : -SMALL; BILATERAL: EACH IS 17 X 8 X 3 MM GENERAL COMMENT (GC) : -BLOOD FILM TAKEN MUSCLE, SKELETAL (SM) : -ATROPHY,-MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-SLIGHT -DILATATION / CYST, FOLLICULAR,-PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, NONGL (SU) : -ACANTHOSIS / HYPERKERATOSIS,MODERATELY SEVERE -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE SEMINAL VESICLES (SV) : -SECRETION, DECREASED,-PRESENT EPIDIDYMIDES (EP) : -IMMATURE SPERM FORMS,-MINIMAL CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 2689 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 934 ANIMAL NUMBER: C92810 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/21/00 STUDY DAY OF DEATH: 673 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 419.9 GRAMS DATE AND TIME OF NECROPSY: 02/21/00 14:45 PROSECTOR: JERALD W. JOHNSON RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAr /i LIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LN, MESENTERIC (MS), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), HEMATO NEOPLASIA (HN) 2690 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 935 ANIMAL NUMBER: C92811 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/25/98 STUDY DAY OF DEATH: 250 STUDY WEEK OF DEATH: 36 TERMINAL BODY WEIGHT: 608.8 GRAMS DATE AND TIME OF NECROPSY: 12/25/98 11:31 PROSECTOR: STEVE VAN ADESTINE RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; LIMITED USE; LIMBS-HIND; VERIFIED AT NECROPSY: NOT APPLICABLE >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY LIVER (LI) : -LARGE; ALL LOBES: MARGINS ARE ROUNDED -MOTTLED; ALL LOBES: RED AND TAN SPLEEN (SP) : -LARGE; 54 X 12 X 6 MM HISTOPATHOLOGY LIVER (LI) : -VACUOLATION, HEPATOCELLULAR, MIDZONAL / CENTRILOBULAR,-MODERATELY SEVERE -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT -DEGENERATION / NECROSIS, CENTRILOBULAR,-MODERATELY SEVERE KIDNEY (KD) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MINIMAL -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT LN, MESENTERIC (MS) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT BONE, FEMUR (FE) : -X-HEMATOPOIETIC NEOPLASIA (SEE HN),NEOPLASM PRESENT MARROW, FEMUR (FM) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT 2691 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 936 ANIMAL NUMBER: C92811 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/25/98 STUDY DAY OF DEATH: 250 STUDY WEEK OF DEATH: 36 TERMINAL BODY WEIGHT: 608.8 GRAMS DATE AND TIME OF NECROPSY: 12/25/98 11:31 PROSECTOR: STEVE VAN ADESTINE RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY LN, MEDIASTINAL (ML) : -LARGE; UP TO 10 X 5 X 4 MM (CAP 21) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN MARROW, STERNUM (SE) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT BONE, STERNUM (SB) : -X-HEMATOPOIETIC NEOPLASIA (SEE HN),NEOPLASM PRESENT HEMATO NEOPLASIA (HN) : -M-LYMPHOMA,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, HEMATOPOIETIC,-PRESENT LN, MEDIASTINAL (ML) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT 2692 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 937 ANIMAL NUMBER: C92811 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/25/98 STUDY DAY OF DEATH: 250 STUDY WEEK OF DEATH: 36 TERMINAL BODY WEIGHT: 608.8 GRAMS DATE AND TIME OF NECROPSY: 12/25/98 11:31 PROSECTOR: STEVE VAN ADESTINE RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUm /s KULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), TESTIS (TE), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP) 2693 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 938 ANIMAL NUMBER: C92812 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 490.8 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 13:30 PROSECTOR: STEVE VAN ADESTINE RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT' (%) LF ADRENAL (AD0) RT ADRENAL (AD1) BRAIN (BR) LF KIDNEY (KD0) RT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LF TESTIS (TE0) RT TESTIS (TE1) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) .0252 .0249 2.1817 2.0210 1.8963 21.4730 1.8765 .7606 1.8219 1.7586 .0121 .0081 .0051 % .0051 % .4445 % .4118 % .3864 % 4.3751 % .3823 % .1550 % .3712 % .3583 % .0025 % .0017 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0116 .0114 1.0000 .9263 .8692 9.8423 .8601 .3486 .8351 .8061 .0055 .0037 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-MODERATE -VACUOLATION, HEPATOCELLULAR, MIDZONAL / CENTRILOBULAR,-SLIGHT KIDNEY (KD) : -DILATATION, PELVIC,-SLIGHT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2694 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 939 ANIMAL NUMBER: C92812 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 490.8 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 13:30 PROSECTOR: STEVE VAN ADESTINE RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE) 2695 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 940 ANIMAL NUMBER: C92813 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/13/00 STUDY DAY OF DEATH: 694 STUDY WEEK OF DEATH: 100 TERMINAL BODY WEIGHT: 463.5 GRAMS DATE AND TIME OF NECROPSY: 03/13/00 13:45 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); DORSAL; WEEK 87; 10 X 10MM; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; DORSAL; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; PAW-HINDRIGHT; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: NOT OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >EYES; SQUINTED; PERI-ORBITAL; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) KIDNEY (KD) : -ROUGH SURFACE, DIFFUSE; BILATERAL -LARGE; BILATERAL: EACH IS 29 X 18 X 13 MM -DIFFUSELY LIGHT; BILATERAL: TAN PANCREAS (PA) : -MASS(ES); DIFFUSE: TWO MASSES, BOTH ARE 8 X 5 X 4 MM, BOTH ARE TAN, RED AND SOFT; BOTH ARE SAME ON CUT SURFACE (CAP 24); REGIONAL LN (PANCREATIC) NOT IDENTIFIED HISTOPATHOLOGY BRAIN (BR) : >UNREMARKABLE LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-SLIGHT -HYPERPLASIA, BILE DUCT,-MINIMAL -FIBROSIS, PERIBILIARY,-MINIMAL -MITOSIS, HEPATOCELLULAR, INCREASED,MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SEVERE -DILATATION, PELVIC,-MINIMAL PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-SEVERE -M-CARCINOMA, ISLET CELL,- 2 NEOPLASMS PRESENT ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-SLIGHT -PIGMENT,-SLIGHT 2696 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 941 ANIMAL NUMBER: C92813 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/13/00 STUDY DAY OF DEATH: 694 STUDY WEEK OF DEATH: 100 TERMINAL BODY WEIGHT: 463.5 GRAMS DATE AND TIME OF NECROPSY: 03/13/00 13:45 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 7 X 5 X 4 MM, DARK RED, TAN AND SOFT SKIN (SK) : -MASS(ES); LEFT DORSAL THORACIC REGION: SINGLE MASS, 10 MM IN DIAMETER, TAN, DARK RED, AND FIRM; CUT SURFACE EXUDES A TAN, CASEOUS SEMISOLID; COLLECTED; OVERLYING AREA HAS A DARK RED AND DRY CRUSTED AREA, 7 X 4 MM; COLLECTED INTACT; REGIONAL LN (SEE AXILLARY) ADRENAL, MEDULLA (MA) : -HYPERPLASIA, MEDULLARY,-MODERATELY SEVERE TESTIS (TE) : -INFLAMMATION, VASCULAR,-MODERATE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATELY SEVERE LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL HEART (HT) : -CARDIOMYOPATHY,-SLIGHT PARATHYROID (PT) : -HYPERPLASIA,-MODERATE PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT SKIN (SK) : -B-KERATOACANTHOMA,-NEOPLASM PRESENT 2697 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 942 ANIMAL NUMBER: C92813 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/13/00 STUDY DAY OF DEATH: 694 STUDY WEEK OF DEATH: 100 TERMINAL BODY WEIGHT: 463.5 GRAMS DATE AND TIME OF NECROPSY: 03/13/00 13:45 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PROSTATE (PR) : -NODULAR; PROXIMAL, SURROUNDING URETHRA: GREEN LN, AXILLARY (AX) : -DIFFUSELY RED; BILATERAL: DARK RED (CAP 23) LN, RENAL (RL) : -DIFFUSELY RED; BILATERAL: DARK RED (CAP 22) FOOT/FOOTPAD (FP) : -CRUSTED AREA(S); RIGHT HINDFOOT: SINGLE DARK RED AND DRY AREA, 5 MM IN DIAMETER; COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE EPIDIDYMIDES (EP) : -IMMATURE SPERM FORMS,-MINIMAL CAUSE OF DEATH (CD) : -NEPHROPATHY,-PRESENT LN, AXILLARY (AX) : -HEMORRHAGE,-PRESENT -HYPERPLASIA, LYMPHOPLASMACYTIC,MODERATE LN, RENAL (RL) : -HEMORRHAGE,-PRESENT MAMMARY, MALE (MM) : -GALACTOCELE,-PRESENT FOOT/FOOTPAD (FP) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE -ACANTHOSIS / HYPERKERATOSIS,-MODERATE 2698 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 943 ANIMAL NUMBER: C92813 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/13/00 STUDY DAY OF DEATH: 694 STUDY WEEK OF DEATH: 100 TERMINAL BODY WEIGHT: 463.5 GRAMS DATE AND TIME OF NECROPSY: 03/13/00 13:45 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), SPLEEN (SP), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, INGUINAL (IN), LN, OTHER (LN), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), SEMINAL VESICLES (SV), HEMATO NEOPLASIA (HN) 2699 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 944 ANIMAL NUMBER: C92814 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 590.5 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 10:50 PROSECTOR: JAMES T. CALLEN RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-MODERATE -VACUOLATION, HEPATOCELLULAR, MIDZONAL / CENTRILOBULAR,-MODERATELY SEVERE -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-SLIGHT -DEGENERATION, CYSTIC,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SLIGHT SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SLIGHT ADRENAL, CORTEX (AC) : -HYPERTROPHY, CORTICAL CELL,-MODERATE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MINIMAL HEART (HT) : -CARDIOMYOPATHY,-MINIMAL 2700 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 945 ANIMAL NUMBER: C92814 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 590.5 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 10:50 PROSECTOR: JAMES T. CALLEN RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -MALOCCLUSION; BILATERAL LOWER INCISORS THYROID (TY) : -HYPERPLASIA, C-CELL,-SLIGHT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2701 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 946 ANIMAL NUMBER: C92814 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 590.5 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 10:50 PROSECTOR: JAMES T. CALLEN RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2702 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 947 ANIMAL NUMBER: C92815 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 722.6 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 11:09 PROSECTOR: CHANESTA ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: NOT OBSERVED >APPEARANCE; MISSING; TEETH; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY HISTOPATHOLOGY EYE (EY) : -DYSTROPHY, CORNEAL IN EARLY STAGES, CONSISTS PRIMARILY OF FOCAL MINERALIZATION,-MINIMAL LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-SLIGHT -NECROSIS, INDIVIDUAL HEPATOCYTE,MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MODERATE -DEGENERATION, CYSTIC,-SLIGHT -HYPERPLASIA, BILE DUCT,-MINIMAL -FIBROSIS, PERIBILIARY,-MINIMAL -B-ADENOMA, HEPATOCELLULAR,-NEOPLASM PRESENT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MODERATE SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE 2703 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 948 ANIMAL NUMBER: C92815 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 722.6 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 11:09 PROSECTOR: CHANESTA ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY ADRENAL, CORTEX (AC) : -MASS(ES); LEFT, INVOLVING ENTIRE GLAND: 10 X 8 X 4 MM, RED, DARK RED, TAN, AND SOFT; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (SEE RENAL) PARATHYROID (PT) : -LARGE; LEFT: 3 MM IN DIAMETER (RIGHT IS 1 MM IN DIAMETER) PANCREAS (PA) : -ATROPHY, ACINAR,-SLIGHT -M-CARCINOMA, ISLET CELL,-NEOPLASM PRESENT ADRENAL, CORTEX (AC) : -HYPERTROPHY, CORTICAL CELL,-SLIGHT -PIGMENT,-SLIGHT ADRENAL, MEDULLA (MA) : -M-MALIGNANT PHEOCHROMOCYTOMA,-NEOPLASM PRESENT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MINIMAL LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL HEART (HT) : -CARDIOMYOPATHY,-SLIGHT THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PARATHYROID (PT) : >UNREMARKABLE PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT 2704 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 949 ANIMAL NUMBER: C92815 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 722.6 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 11:09 PROSECTOR: CHANESTA ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -RED FOCUS(I)/AREA(S); LEFT ASPECT: DARK RED AREA, 3 MM IN DIAMETER LN, RENAL (RL) : -DIFFUSELY RED; BILATERAL: DARK RED (CAP 22) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -TEETH MISSING/BROKEN/WORN; BILATERAL UPPER INCISORS: BROKEN OFF AT BASE PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, RENAL (RL) : -HEMORRHAGE,-PRESENT -DILATATION, SINUSOIDAL,-MODERATELY SEVERE 2705 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 950 ANIMAL NUMBER: C92815 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 722.6 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 11:09 PROSECTOR: CHANESTA ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: BRAIN (BR), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2706 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 951 ANIMAL NUMBER: C92816 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 07/09/99 STUDY DAY OF DEATH: 446 STUDY WEEK OF DEATH: 64 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 07/09/99 15:29 PROSECTOR: JERALD W. JOHNSON RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-SLIGHT -VACUOLATION, HEPATOCELLULAR,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MINIMAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATE LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL HEART (HT) : -CARDIOMYOPATHY,-MINIMAL -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL THYROID (TY) : >AUTOLYTIC STOMACH, GL (ST) : >AUTOLYTIC 2707 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 952 ANIMAL NUMBER: C92816 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 07/09/99 STUDY DAY OF DEATH: 446 STUDY WEEK OF DEATH: 64 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 07/09/99 15:29 PROSECTOR: JERALD W. JOHNSON RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY LN, MEDIASTINAL (ML) : -LARGE; UP TO 3 X 2 X 1 MM (CAP 21) -DIFFUSELY RED; DARK RED AORTA (AO) : -MASS(ES); INVOLVING ENTIRE THORACIC AORTA: SINGLE MASS, 35 X 30 X 15 MM, RED, DARK RED, TAN, LOBULATED AND SOFT; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (SEE MEDIASTINAL) GENERAL COMMENT (GC) : -FLUID-THORACIC CAVITY; CONTAINS A LARGE AMOUNT OF DARK RED FLUID JEJUNUM (JE) : >AUTOLYTIC ILEUM (IL) : >AUTOLYTIC CECUM (CE) : >AUTOLYTIC COLON (CO) : >AUTOLYTIC URINARY BLADDER (UB) : >AUTOLYTIC PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL CAUSE OF DEATH (CD) : -NEOPLASM, THORACIC CAVITY,-PRESENT >NOTE:>DIAGNOSIS LISTED UNDER AORTA LN, MEDIASTINAL (ML) : -HEMORRHAGE,-PRESENT AORTA (AO) : -M-LIPOSARCOMA,-NEOPLASM PRESENT 2708 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 953 ANIMAL NUMBER: C92816 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 07/09/99 STUDY DAY OF DEATH: 446 STUDY WEEK OF DEATH: 64 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 07/09/99 15:29 PROSECTOR: JERALD W. JOHNSON RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), RECTUM (RE), SKIN (SK), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) (TE), 2709 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 954 ANIMAL NUMBER: C92817 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 958.5 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 9:22 PROSECTOR: JOHN S. HALFORD RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT' (%) LF ADRENAL (AD0) RT ADRENAL (AD1) BRAIN (BR) LF KIDNEY (KD0) RT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LF TESTIS (TE0) RT TESTIS (TE1) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) .0360 .0306 2.3859 2.7116 2.7885 31.0099 2.1009 1.1429 1.9473 1.8553 .0216 .0280 .0038 % .0032 % .2489 % .2829 % .2909 % 3.2353 % .2192 % .1192 % .2032 % .1936 % .0023 % .0029 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0151 .0128 1.0000 1.1365 1.1687 12.9971 .8805 .4790 .8162 .7776 .0091 .0117 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-MODERATELY SEVERE -NECROSIS, INDIVIDUAL HEPATOCYTE,MINIMAL -VACUOLATION, HEPATOCELLULAR, MIDZONAL / CENTRILOBULAR,-MODERATE KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL 2710 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 955 ANIMAL NUMBER: C92817 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 958.5 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 9:22 PROSECTOR: JOHN S. HALFORD RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -CYST(S); MID POSTERIOR ASPECT: SINGLE CLEAR FLUID FILLED CYST, 2 MM IN DIAMETER GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN -ANIMAL OBESE SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SLIGHT PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL THYMUS (TH) : -HEMORRHAGE,-PRESENT LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT -HYPERPLASIA,-MINIMAL PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2711 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 956 ANIMAL NUMBER: C92817 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 958.5 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 9:22 PROSECTOR: JOHN S. HALFORD RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAr /i LIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2712 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 957 ANIMAL NUMBER: C92818 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 720.9 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 9:38 PROSECTOR: TODD ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; SWOLLEN; PAW-HIND-RIGHT; VERIFIED AT NECROPSY: NOT OBSERVED OBSERVATIONS NECROPSY HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-SLIGHT -GRANULAR CYTOPLASM, EOSINOPHILIC, CENTRILOBULAR,-SLIGHT -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-MODERATE -DEGENERATION, CYSTIC,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT ADRENAL, CORTEX (AC) : -PIGMENT,-SLIGHT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATELY SEVERE LUNG (LU) : -MINERALIZATION, VASCULAR,-SLIGHT HEART (HT) : -CARDIOMYOPATHY,-MINIMAL 2713 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 958 ANIMAL NUMBER: C92818 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 720.9 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 9:38 PROSECTOR: TODD ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -RED FOCUS(I)/AREA(S); LEFT ASPECT: SINGLE DARK RED AREA, 3 X 2 MM STOMACH, GL (ST) : -THICKENED WALL; MUCOSA: DIFFUSE GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN THYROID (TY) : -B-ADENOMA, FOLLICULAR CELL,-NEOPLASM PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT -HYPERPLASIA,-SLIGHT STOMACH, GL (ST) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT -FIBROSIS,-SLIGHT URINARY BLADDER (UB) : -PROTEINACEOUS MATERIAL, LUMINAL,PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2714 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 959 ANIMAL NUMBER: C92818 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 720.9 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 9:38 PROSECTOR: TODD ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAr /i LIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2715 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 960 ANIMAL NUMBER: C92819 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 04/15/00 STUDY DAY OF DEATH: 727 STUDY WEEK OF DEATH: 104 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 04/15/00 8:44 PROSECTOR: JARED FISHEL RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); DORSAL-CERVICAL-LEFT; WEEK 25; 60 X 50MM; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; PAW-HINDLEFT; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; DORSALCERVICAL-LEFT; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -MOTTLED; ALL LOBES: RED AND TAN ADRENAL, CORTEX (AC) : -LARGE; RIGHT: 22 MM IN DIAMETER (LEFT IS 5 X 3 X 2 MM) HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-SLIGHT -DEGENERATION, CYSTIC,-SLIGHT -DEGENERATION / NECROSIS, CENTRILOBULAR,-SEVERE -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE -N-CARCINOMA,-FROM ADRENAL, CORTEX KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MODERATE -THROMBUS, GLOMERULAR,-SEVERE -DEGENERATION TUBULAR,-MODERATELY SEVERE SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE PANCREAS (PA) : -INFILTRATION, FAT, INTERSTITIAL,MODERATELY SEVERE -NECROSIS,-MODERATE ADRENAL, CORTEX (AC) : -DEGENERATION / NECROSIS, UNILATERAL,SEVERE -M-CARCINOMA,-NEOPLASM PRESENT 2716 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 961 ANIMAL NUMBER: C92819 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 04/15/00 STUDY DAY OF DEATH: 727 STUDY WEEK OF DEATH: 104 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 04/15/00 8:44 PROSECTOR: JARED FISHEL RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY TESTIS (TE) : -SMALL; BILATERAL: EACH IS 17 X 12 X 8 MM; BOTH ARE SOFT LUNG (LU) : -LIGHT FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE TAN AREAS, UP TO 5 X 2 MM PITUITARY (PI) : -LARGE; 7 X 4 X 3 MM; APPEARS SWOLLEN TESTIS (TE) : >UNREMARKABLE MARROW, STERNUM (SE) : -N-CARCINOMA,-FROM ADRENAL, CORTEX THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),SEVERE LUNG (LU) : -MINERALIZATION, VASCULAR,-SLIGHT -N-CARCINOMA,-FROM ADRENAL, CORTEX HEART (HT) : -CARDIOMYOPATHY,-SLIGHT PARATHYROID (PT) : -HYPERPLASIA,-MODERATE PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT JEJUNUM (JE) : >AUTOLYTIC ILEUM (IL) : >AUTOLYTIC CECUM (CE) : >AUTOLYTIC SKIN (SK) : -B-FIBROMA,-NEOPLASM PRESENT URINARY BLADDER (UB) : -HYPERPLASIA, TRANSITIONAL CELL,-SLIGHT -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT 2717 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 962 ANIMAL NUMBER: C92819 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 04/15/00 STUDY DAY OF DEATH: 727 STUDY WEEK OF DEATH: 104 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 04/15/00 8:44 PROSECTOR: JARED FISHEL RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY SEMINAL VESICLES (SV) : -SMALL; BILATERAL: EACH IS 15 X 7 X 2 MM LN, AXILLARY (AX) : -DIFFUSELY RED; RIGHT: DARK RED (CAP 22) (LEFT NOT IDENTIFIED) MAMMARY, MALE (MM) : -MASS(ES); LEFT DORSAL THORACIC REGION: SINGLE MASS, 70 X 40 X 40 MM, TAN, FIRM AND LOBULATED; SAME ON CUT SURFACE; PORTION COLLECTED; REGIONAL LN (SEE AXILLARY); OVERLYING SKIN HAS A SINGLE DARK RED AND MOIST ABRADED AREA, 12 MM IN DIAMETER; COLLECTED WITH MASS FOOT/FOOTPAD (FP) : -CRUSTED AREA(S); LEFT HINDFOOT: SINGLE DARK RED AND DRY CRUSTED AREA, 15 MM IN DIAMETER; COLLECTED PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SEVERE SEMINAL VESICLES (SV) : -ATROPHY,-SEVERE EPIDIDYMIDES (EP) : -IMMATURE SPERM FORMS,-SLIGHT CAUSE OF DEATH (CD) : -NEOPLASM, ADRENAL,-PRESENT LN, AXILLARY (AX) : -HEMORRHAGE,-PRESENT MAMMARY, MALE (MM) : >NOT REQUIRED TO BE EXAMINED FOR ANIMAL >NOTE:>SEE SKIN FOR DIAGNOSIS OF MASS FOOT/FOOTPAD (FP) : -INFLAMMATION, CHRONIC-ACTIVE,-SEVERE -ULCERATION,-SEVERE -ACANTHOSIS / HYPERKERATOSIS,-MODERATE -FIBROSIS,-MODERATE -BACTERIA,-PRESENT 2718 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 963 ANIMAL NUMBER: C92819 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 04/15/00 STUDY DAY OF DEATH: 727 STUDY WEEK OF DEATH: 104 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 04/15/00 8:44 PROSECTOR: JARED FISHEL RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; MOIST BROWN MATERIAL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL PREPUTIAL GLAND (PG), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) (AS), THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), COLON (CO), RECTUM (RE), HEMATO NEOPLASIA (HN) 2719 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 964 ANIMAL NUMBER: C92820 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 503.2 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 10:17 PROSECTOR: KEVIN BILLINGS RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT' (%) LF ADRENAL (AD0) RT ADRENAL (AD1) BRAIN (BR) LF KIDNEY (KD0) RT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LF TESTIS (TE0) RT TESTIS (TE1) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) .0240 .0229 2.2863 2.2802 2.2533 17.6865 1.8264 .6612 1.7905 1.7871 .0120 .0131 .0048 % .0046 % .4544 % .4531 % .4478 % 3.5148 % .3630 % .1314 % .3558 % .3551 % .0024 % .0026 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0105 .0100 1.0000 .9973 .9856 7.7359 .7988 .2892 .7831 .7817 .0052 .0057 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED LIVER (LI) : -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-MODERATELY SEVERE -VACUOLATION, HEPATOCELLULAR,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, TUBULAR,-MINIMAL MARROW, FEMUR (FM) : -HYPOCELLULAR, MARROW,-SLIGHT 2720 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 965 ANIMAL NUMBER: C92820 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 503.2 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 10:17 PROSECTOR: KEVIN BILLINGS RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN -MALOCCLUSION; BILATERAL: UPPER AND LOWER INCISORS THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),SLIGHT HEART (HT) : -CARDIOMYOPATHY,-SLIGHT STOMACH, GL (ST) : -EROSION,-SLIGHT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2721 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 966 ANIMAL NUMBER: C92820 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 503.2 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 10:17 PROSECTOR: KEVIN BILLINGS RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2722 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 967 ANIMAL NUMBER: C92821 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 04/24/00 STUDY DAY OF DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 782.7 GRAMS DATE AND TIME OF NECROPSY: 04/24/00 8:11 PROSECTOR: TROY ELLENBECKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; PAW-HINDLEFT; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; MISSING; TEETH; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; SWOLLEN; PAW-HIND-LEFT; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE RED AREAS, UP TO 3 X 2 MM; COLLECTED ON ROUTINE SECTIONS PITUITARY (PI) : -RED FOCUS(I)/AREA(S); RIGHT ASPECT: SINGLE DARK RED AREA, 3 X 2 MM FOOT/FOOTPAD (FP) : -CRUSTED AREA(S); LEFT HINDFOOT: SINGLE DRY BROWN CRUSTED AREA, 3 MM IN DIAMETER; JOINT APPEARS SWOLLEN; COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL -DEGENERATION, CYSTIC,-SLIGHT -NECROSIS, COAGULATIVE,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-SLIGHT LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT FOOT/FOOTPAD (FP) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE -FIBROSIS,-SEVERE -HYPERPLASIA, BONE,-MODERATELY SEVERE 2723 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 968 ANIMAL NUMBER: C92821 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 04/24/00 STUDY DAY OF DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 782.7 GRAMS DATE AND TIME OF NECROPSY: 04/24/00 8:11 PROSECTOR: TROY ELLENBECKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN -MALOCCLUSION; BILATERAL UPPER INCISORS -TEETH MISSING/BROKEN/WORN; BILATERAL UPPER INCISORS: BROKEN OFF AT BASE -DISCHARGE-OCULAR; LEFT: DRY RED MATERIAL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), THYROID (TY), PARATHYROID (PT), URINARY BLADDER (UB) 2724 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 969 ANIMAL NUMBER: C92822 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/06/99 STUDY DAY OF DEATH: 566 STUDY WEEK OF DEATH: 81 TERMINAL BODY WEIGHT: 485.0 GRAMS DATE AND TIME OF NECROPSY: 11/06/99 13:38 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; ATAXIC; VERIFIED AT NECROPSY: NOT APPLICABLE >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; HEAD TILT; LEFT; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) HISTOPATHOLOGY BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -DEGENERATION, CYSTIC,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -HYPERPLASIA, TRANSITIONAL CELL,MINIMAL -INFLAMMATION, SUPPURATIVE, PELVIC,MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-MINIMAL -PIGMENT,-MINIMAL TESTIS (TE) : -ATROPHY/DEGENERATION,-SEVERE >NOTE:>UNILATERAL 2725 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 970 ANIMAL NUMBER: C92822 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/06/99 STUDY DAY OF DEATH: 566 STUDY WEEK OF DEATH: 81 TERMINAL BODY WEIGHT: 485.0 GRAMS DATE AND TIME OF NECROPSY: 11/06/99 13:38 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 12 X 11 X 10 MM, RED, DARK RED, GREY, TAN, LOBULATED, AND SOFT PROSTATE (PR) : -NODULAR; DIFFUSE: GREEN AND FIRM -LARGE; 35 X 21 X 14 MM SEMINAL VESICLES (SV) : -LARGE; BILATERAL: EACH IS 31 X 21 X 9 MM -GELATINOUS; BILATERAL: CLEAR AND SOFT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),SEVERE LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL THYROID (TY) : -B-ADENOMA, FOLLICULAR CELL,-NEOPLASM PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-SLIGHT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE SEMINAL VESICLES (SV) : -SECRETION, INCREASED,-PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 2726 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 971 ANIMAL NUMBER: C92822 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/06/99 STUDY DAY OF DEATH: 566 STUDY WEEK OF DEATH: 81 TERMINAL BODY WEIGHT: 485.0 GRAMS DATE AND TIME OF NECROPSY: 11/06/99 13:38 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2727 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 972 ANIMAL NUMBER: C92823 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 04/24/00 STUDY DAY OF DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 791.7 GRAMS DATE AND TIME OF NECROPSY: 04/24/00 9:00 PROSECTOR: TROY ELLENBECKER RECORDER: TROY ELLENBECKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; MISSING; TEETH; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) THYROID (TY) : -LARGE; BILATERAL:: EACH IS 8 X 4 X 3 MM PITUITARY (PI) : -MASS(ES) ; 11 X 6 X 4 MM, RED, TAN AND SP AOCFirTp HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-SLIGHT -HYPERPLASIA, BILE DUCT,-MINIMAL -FIBROSIS, PERIBILIARY,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, PERIPORTAL,-SLIGHT -VACUOLATION, HEPATOCELLULAR,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SLIGHT PANCREAS (PA) : -INFILTRATION, FAT, INTERSTITIAL,MINIMAL LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL THYROID (TY) : -B-ADENOMA, FOLLICULAR CELL,-NEOPLASM PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT 2728 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 973 ANIMAL NUMBER: C92823 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 04/24/00 STUDY DAY OF DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 791.7 GRAMS DATE AND TIME OF NECROPSY: 04/24/00 9:00 PROSECTOR: TROY ELLENBECKER RECORDER: TROY ELLENBECKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY STOMACH, GL (ST) : -THICKENED WALL; MUCOSA: DIFFUSE GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -MALOCCLUSION; BILATERAL, UPPER INCISORS -TEETH MISSING/BROKEN/WORN; BILATERAL, UPPER INCISORS: BROKEN OFF AT BASE STOMACH, GL (ST) : -HYPERPLASIA, EPITHELIAL, GLANDULAR,SLIGHT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2729 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 974 ANIMAL NUMBER: C92823 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 04/24/00 STUDY DAY OF DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 791.7 GRAMS DATE AND TIME OF NECROPSY: 04/24/00 9:00 PROSECTOR: TROY ELLENBECKER RECORDER: TROY ELLENBECKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: TESTIS (TE), PARATHYROID (PT), URINARY BLADDER (UB) 2730 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 975 ANIMAL NUMBER: C92824 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 02/11/00 STUDY DAY OF DEATH: 663 STUDY WEEK OF DEATH: 95 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 02/11/00 7:33 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); AXILLARY REGION-RIGHT; WEEK 66; 50 X 70MM; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE DARK RED AREAS, UP TO 4 MM IN DIAMETER; COLLECTED ON ROUTINE SECTIONS KIDNEY (KD) : -LARGE PELVIS(ES); BILATERAL -GRANULAR MATERIAL; BILATERAL: MULTIPLE TAN AND HARD GRANULES, UP TO 1 MM IN DIAMETER -DIFFUSELY LIGHT; BILATERAL: TAN -SEMIFLUID MATERIAL-PELVIS; LEFT: LIGHT GREEN SPLEEN (SP) : -DIFFUSELY LIGHT; TAN HISTOPATHOLOGY BRAIN (BR) : >UNREMARKABLE LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -DEGENERATION, CYSTIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-SLIGHT -HYPERTROPHY, HEPATOCELLULAR, PERIPORTAL,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SEVERE -DILATATION, PELVIC,-MODERATE -REGENERATION, TUBULAR EPITHELIUM,MODERATE -INFLAMMATION, CHRONIC-ACTIVE, PELVIC,SEVERE -ULCERATION, TRANSITIONAL EPITHELIUM, PELVIC,-SEVERE SPLEEN (SP) : ^UNREMARKABLE ADRENAL, CORTEX (AC) : -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL TESTIS (TE) : -ATROPHY/DEGENERATION,-MINIMAL 2731 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 976 ANIMAL NUMBER: C92824 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 02/11/00 STUDY DAY OF DEATH: 663 STUDY WEEK OF DEATH: 95 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 02/11/00 7:33 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY HEART (HT) : -LIGHT FOCUS(I)/AREA(S); EPICARDIUM OF BOTH VENTRICLES: MULTIPLE TAN AREAS, UP TO 10 X 5 MM PITUITARY (PI) : -MASS(ES); 7 X 6 X 4 MM, DARK RED, TAN AND SOFT STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: MULTIPLE BLACK AREAS, UP TO 1 MM IN DIAMETER CECUM (CE) : -RED FOCUS(I)/AREA(S); MUCOSA, DIFFUSE: MULTIPLE RED AREAS, UP TO 5 X 4 MM MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,MODERATELY SEVERE THYMUS (TH) : >SECTION EXAMINED; TISSUE NOT PRESENT HEART (HT) : -CARDIOMYOPATHY,-MODERATE -MINERALIZATION, VASCULAR,-MODERATE -MINERALIZATION,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : -MINERALIZATION, GLANDULAR MUCOSA,MINIMAL CECUM (CE) : >AUTOLYTIC COLON (CO) : >AUTOLYTIC URINARY BLADDER (UB) : -HYPERPLASIA, TRANSITIONAL CELL,MODERATE -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SEVERE 2732 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 977 ANIMAL NUMBER: C92824 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 02/11/00 STUDY DAY OF DEATH: 663 STUDY WEEK OF DEATH: 95 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 02/11/00 7:33 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY LN, AXILLARY (AX) : -DIFFUSELY RED; BILATERAL (CAP 24) LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL (CAP 22) MAMMARY, MALE (MM) : -MASS(ES); RIGHT AXILLARY REGION: SINGLE MASS, 60 X 55 X 35 MM, RED, DARK RED, TAN, LOBULATED AND FIRM; SAME ON CUT SURFACE; PORTION COLLECTED; REGIONAL LN (SEE AXILLARY) LN, LUMBAR/ILIAC (LM) : -MOTTLED; BILATERAL: DARK BROWN AND TAN (CAP 25) SEMINAL VESICLES (SV) : -ATROPHY,-MODERATELY SEVERE EPIDIDYMIDES (EP) : -IMMATURE SPERM FORMS,-SLIGHT CAUSE OF DEATH (CD) : -INFECTION / NECROSIS / CALCULUS, URINARY TRACT,-PRESENT LN, AXILLARY (AX) : -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL -CONGESTION,-PRESENT LN, MANDIBULAR (MN) : -LYMPHANGIECTASIS,-PRESENT -CONGESTION,-PRESENT MAMMARY, MALE (MM) : -M-CARCINOMA,-NEOPLASM PRESENT LN, LUMBAR/ILIAC (LM) : -LYMPHANGIECTASIS,-PRESENT 2733 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 978 ANIMAL NUMBER: C92824 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 02/11/00 STUDY DAY OF DEATH: 663 STUDY WEEK OF DEATH: 95 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 02/11/00 7:33 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), COAGULATING GL (CG), TAIL (TI), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG), GENERAL COMMENT (GC) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), RECTUM (RE), SKIN (SK), HEMATO NEOPLASIA (HN) 2734 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 979 ANIMAL NUMBER: C92825 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 02/01/00 STUDY DAY OF DEATH: 653 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 02/01/00 7:57 PROSECTOR: STEVE VAN ADESTINE RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -MOTTLED; ALL LOBES: RED AND TAN KIDNEY (KD) : -LARGE PELVIS(ES); BILATERAL HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -DEGENERATION, CYSTIC,-MINIMAL -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SLIGHT -DILATATION, PELVIC,-SLIGHT SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SLIGHT PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-SLIGHT -INFILTRATION, FAT, INTERSTITIAL,SLIGHT ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-MINIMAL -ANGIECTASIS,-SLIGHT TESTIS (TE) : -INFLAMMATION, VASCULAR,-MINIMAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATELY SEVERE 2735 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 980 ANIMAL NUMBER: C92825 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 02/01/00 STUDY DAY OF DEATH: 653 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 02/01/00 7:57 PROSECTOR: STEVE VAN ADESTINE RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY THYROID (TY) : -MASS(ES); LEFT, INVOLVING ENTIRE GLAND: SINGLE MASS, 15 X 13 X 13 MM, RED, DARK RED, GRAY, LOBULATED, AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (SEE MANDIBULAR) LN, MEDIASTINAL (ML) : -LARGE; UP TO 4 X 3 X 2 MM -DIFFUSELY RED; DARK RED (CAP 22) LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL (CAP 23) LUNG (LU) : -N-SARCOMA,-NEOPLASM PRESENT HEART (HT) : -CARDIOMYOPATHY,-MINIMAL THYROID (TY) : -M-CARCINOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, NONGL (SU) : -ACANTHOSIS / HYPERKERATOSIS,-MINIMAL CECUM (CE) : >AUTOLYTIC PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT CAUSE OF DEATH (CD) : -NEOPLASM, THORACIC CAVITY,-PRESENT LN, MEDIASTINAL (ML) : -HEMORRHAGE,-PRESENT LN, MANDIBULAR (MN) : -CONGESTION,-PRESENT PERICARDIAL SAC (PD) : -M-SARCOMA, UNDIFFERENTIATED,-NEOPLASM PRESENT 2736 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 981 ANIMAL NUMBER: C92825 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 02/01/00 STUDY DAY OF DEATH: 653 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 02/01/00 7:57 PROSECTOR: STEVE VAN ADESTINE RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PERICARDIAL SAC (PD) : -MASS(ES); INVOLVING ENTIRE ORGAN: SINGLE MASS, 53 X 45 X 38 MM, RED, DARK RED, TAN, GRAY, AND SOFT; ON SECTION EXUDES A DARK RED FLUID; COLLECTED INTACT; REGIONAL LN (SEE MEDIASTINAL) GENERAL COMMENT (GC) : -FLUID-THORACIC CAVITY; DARK RED, GELATINOUS SEMIFLUID 2737 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 982 ANIMAL NUMBER: C92825 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 02/01/00 STUDY DAY OF DEATH: 653 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 02/01/00 7:57 PROSECTOR: STEVE VAN ADESTINE RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) (ST), 2738 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 983 ANIMAL NUMBER: C92826 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 05/09/99 STUDY DAY OF DEATH: 385 STUDY WEEK OF DEATH: 55 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 05/09/99 9:01 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK 45; VERIFIED AT NECROPSY: OBSERVED >DISCHARGE; CLEAR ORAL; VERIFIED AT NECROPSY: OBSERVED >RESPIRATION; AUDIBLE; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) THYMUS (TH) : -RED FOCUS(I)/AREA(S); DIFFUSE: MULTIPLE, DARK RED, PINPOINT FOCI HISTOPATHOLOGY BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-MODERATE -VACUOLATION, HEPATOCELLULAR, MIDZONAL / CENTRILOBULAR,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL THYMUS (TH) : -HEMORRHAGE,-PRESENT -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),SLIGHT 2739 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 984 ANIMAL NUMBER: C92826 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 05/09/99 STUDY DAY OF DEATH: 385 STUDY WEEK OF DEATH: 55 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 05/09/99 9:01 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 10 X 8 X 8 MM, RED, DARK RED, GRAY, AND SOFT STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: SINGLE BROWN AREA, 4 X 2 MM SEMINAL VESICLES (SV) : -GELATINOUS; BILATERAL: CLEAR MAMMARY, MALE (MM) : -CYST(S); LEFT INGUINAL REGION: SINGLE GREENISH-TAN, CREAMY SEMIFLUID FILLED CYST, 8 MM IN DIAMETER (CAP 21); POSSIBLY CONSISTENT WITH MASS NOTED IN CLINICAL OBSERVATIONS GENERAL COMMENT (GC) : -DISCHARGE-ORAL; CLEAR AND MOIST LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : >UNREMARKABLE RECTUM (RE) : -PARASITISM,-PRESENT SKIN (SK) : -INFLAMMATION, CHRONIC-ACTIVE,MODERATELY SEVERE PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL SEMINAL VESICLES (SV) : >UNREMARKABLE CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT MAMMARY, MALE (MM) : >NOT REQUIRED TO BE EXAMINED FOR ANIMAL >NOTE:>SEE SKIN FOR DIAGNOSIS 2740 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 985 ANIMAL NUMBER: C92826 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 05/09/99 STUDY DAY OF DEATH: 385 STUDY WEEK OF DEATH: 55 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 05/09/99 9:01 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), URINARY BLADDER (UB), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) (SM), 2741 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 986 ANIMAL NUMBER: C92827 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 04/24/00 STUDY DAY OF DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 745.5 GRAMS DATE AND TIME OF NECROPSY: 04/24/00 9:35 PROSECTOR: JOHN S. HALFORD RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); DORSAL; WEEK 92; 10 X 10MM; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; DORSAL; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE RED AREAS, UP TO 2 X 1 MM; COLLECTED ON ROUTINE SECTIONS THYMUS (TH) : -MISSING/NOT IDENTIFIED; NOT POSITIVELY IDENTIFIED; POSSIBLE REMNANTS COLLECTED HISTOPATHOLOGY BRAIN (BR) : >UNREMARKABLE LIVER (LI) : -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL -DEGENERATION, CYSTIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL -FIBROSIS, PERIBILIARY,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL PANCREAS (PA) : -B-ADENOMA, ISLET CELL,- 2 NEOPLASMS PRESENT -INFILTRATION, FAT, INTERSTITIAL,SLIGHT THYMUS (TH) : >NOT REQUIRED TO BE EXAMINED FOR ANIMAL LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL 2742 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 987 ANIMAL NUMBER: C92827 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 04/24/00 STUDY DAY OF DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 745.5 GRAMS DATE AND TIME OF NECROPSY: 04/24/00 9:35 PROSECTOR: JOHN S. HALFORD RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 8 X 6 X 4 MM, RED, DARK RED, GRAY, TAN, CYSTIC, LOBULATED AND SOFT SKIN (SK) : -MASS(ES); MID DORSAL THORACIC REGION: SINGLE MASS, 15 X 11 X 4 MM, RED, TAN AND FIRM; SAME ON CUT SURFACE (CAP 23); REGIONAL LN (AXILLARY) NORMAL (CAP 24); OVERLYING SKIN HAS A SINGLE DRY, TAN CRUSTED AREA, 14 X 10 MM; COLLECTED WITH MASS PREPUTIAL GLAND (PG) : -LARGE; LEFT: 9 X 4 X 4 MM (RIGHT IS 4 X 3 X 3 MM); LEFT EXUDES A GREEN CASEOUS MATERIAL; RIGHT EXUDES A TAN CASEOUS MATERIAL (CAP 22) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT SKIN (SK) : -B-KERATOACANTHOMA,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT PREPUTIAL GLAND (PG) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE -ECTASIA, DUCTAL,-PRESENT 2743 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 988 ANIMAL NUMBER: C92827 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 04/24/00 STUDY DAY OF DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 745.5 GRAMS DATE AND TIME OF NECROPSY: 04/24/00 9:35 PROSECTOR: JOHN S. HALFORD RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: TESTIS (TE), PARATHYROID (PT), URINARY BLADDER (UB), LN, AXILLARY (AX) 2744 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 989 ANIMAL NUMBER: C92828 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/18/99 STUDY DAY OF DEATH: 578 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/18/99 12:47 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; SWOLLEN; TESTIS-LEFT; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY TESTIS (TE) : -MASS(ES); LEFT: SINGLE MASS, 120 X 35 X 35 MM, RED, DARK RED, TAN, GRAY AND FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (SEE LUMBAR) HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -VACUOLATION, HEPATOCELLULAR, MIDZONAL / CENTRILOBULAR,-SLIGHT -DEGENERATION, CYSTIC,-MINIMAL -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL -DEGENERATION / NECROSIS, CENTRILOBULAR,-MODERATE SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATELY SEVERE PANCREAS (PA) : -INFILTRATE, LYMPHOHISTIOCYTIC,-SLIGHT ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-MODERATE -HEMATOPOIESIS, EXTRAMEDULLARY,MODERATE -HYPERTROPHY, CORTICAL CELL,-MODERATELY SEVERE TESTIS (TE) : -M-HEMANGIOSARCOMA,-NEOPLASM PRESENT 2745 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 990 ANIMAL NUMBER: C92828 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/18/99 STUDY DAY OF DEATH: 578 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/18/99 12:47 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -RED FOCUS(I)/AREA(S); LEFT ASPECT: SINGLE DARK RED AREA, 3 X 2 MM STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: MULTIPLE DARK BROWN FOCI, PINPOINT AND UP TO 2 MM IN DIAMETER MARROW, FEMUR (FM) : -HYPERPLASIA, ERYTHROID, MARROW,MODERATE MARROW, STERNUM (SE) : -HYPERPLASIA, ERYTHROID, MARROW,MODERATE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATELY SEVERE LUNG (LU) : -LEUKOCYTES, INCREASED, INTRAVASCULAR,PRESENT HEART (HT) : -CARDIOMYOPATHY,-MINIMAL PARATHYROID (PT) : >SECTION EXAMINED; TISSUE NOT PRESENT PITUITARY (PI) : -HYPERPLASIA,-MODERATE STOMACH, GL (ST) : >UNREMARKABLE JEJUNUM (JE) : >AUTOLYTIC ILEUM (IL) : >AUTOLYTIC 2746 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 991 ANIMAL NUMBER: C92828 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/18/99 STUDY DAY OF DEATH: 578 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/18/99 12:47 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY EPIDIDYMIDES (EP) : >NOTE:>LEFT NOT IDENTIFIED DUE TO MASS ON LEFT TESTIS LN, LUMBAR/ILIAC (LM) : -DIFFUSELY RED; BILATERAL: DARK RED (CAP 22) CECUM (CE) : >AUTOLYTIC EPIDIDYMIDES (EP) : -UNILATERALLY EXAMINED,-PRESENT CAUSE OF DEATH (CD) : -HEMANGIOSARCOMA,-PRESENT LN, LUMBAR/ILIAC (LM) : -HYPERPLASIA, LYMPHOPLASMACYTIC,-SLIGHT -HEMORRHAGE,-PRESENT 2747 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 992 ANIMAL NUMBER: C92828 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/18/99 STUDY DAY OF DEATH: 578 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/18/99 12:47 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG), GENERAL COMMENT (GC) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), HEMATO NEOPLASIA (HN) 2748 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 993 ANIMAL NUMBER: C92829 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 04/24/00 STUDY DAY OF DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 694.8 GRAMS DATE AND TIME OF NECROPSY: 04/24/00 10:52 PROSECTOR: LAURIE J. SCHULLER RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; SWOLLEN; PAW-HIND-RIGHT; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; VENTRAL ABDOMINAL; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) KIDNEY (KD) : -CYST(S); RIGHT CORTICAL SURFACE: SINGLE CLEAR FLUID FILLED CYST, 3 MM IN DIAMETER PANCREAS (PA) : -MASS(ES); NEAR DUODENUM: SINGLE MASS, 22 X 15 X 10 MM, RED, DARK RED, TAN AND FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (ANTERIOR MESENTERIC) NORMAL (CAP 26) TESTIS (TE) : -SMALL; BILATERAL: EACH IS 20 X 15 X 4 MM; BOTH ARE SOFT HISTOPATHOLOGY BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL -DEGENERATION, CYSTIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SLIGHT -CYST,-PRESENT PANCREAS (PA) : -M-CARCINOMA, ISLET CELL,-NEOPLASM PRESENT TESTIS (TE) : >UNREMARKABLE 2749 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 994 ANIMAL NUMBER: C92829 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 04/24/00 STUDY DAY OF DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 694.8 GRAMS DATE AND TIME OF NECROPSY: 04/24/00 10:52 PROSECTOR: LAURIE J. SCHULLER RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 11 X 8 X 6 MM, DARK RED, GRAY AND SOFT STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: MULTIPLE DARK BROWN AREAS, UP TO 3 X 1 MM CECUM (CE) : -THICKENED WALL; DIFFUSE PROSTATE (PR) : -NODULAR; PROXIMAL SURROUNDING URETHRA: GREEN AND FIRM SEMINAL VESICLES (SV) : -SMALL; BILATERAL: EACH IS 15 X 10 X 2 MM -GELATINOUS; BILATERAL: CLEAR LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL -B-ADENOMA, FOLLICULAR CELL,-NEOPLASM PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : >UNREMARKABLE CECUM (CE) : >UNREMARKABLE PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT SEMINAL VESICLES (SV) : -SECRETION, DECREASED,-PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT TAIL (TI) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE -ACANTHOSIS / HYPERKERATOSIS,-SLIGHT 2750 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 995 ANIMAL NUMBER: C92829 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 04/24/00 STUDY DAY OF DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 694.8 GRAMS DATE AND TIME OF NECROPSY: 04/24/00 10:52 PROSECTOR: LAURIE J. SCHULLER RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY TAIL (TI) : -CRUSTED AREA(S); ALONG ENTIRE LENGTH: MULTIPLE BROWN, DRY AREAS, UP TO 2 X 1 MM; COLLECTED PREPUTIAL GLAND (PG) : -LARGE; BILATERAL: EACH IS 12 X 6 X 4 MM; BOTH CONTAIN A LIGHT GREEN SEMISOLID MATERIAL (CAP 25); OVERLYING SKIN HAS A DARK RED, CRUSTED AREA, 10 X 6 MM; COLLECTED INTACT WITH PREPUTIAL GLAND JOINT, OTHER (JT) : -LARGE; RIGHT TARSAL JOINT: 15 MM IN DIAMETER (LEFT IS 9 MM IN DIAMETER); COLLECTED LN, POPLITEAL (PP) : -LARGE; BILATERAL: EACH IS UP TO 10 X 8 X 3 MM (CAP 24) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -GI TRACT-DARK FLUID CONTENT; DISTAL JEJUNUM AND ILEUM: CONTAIN A DARK GREEN SEMIFLUID TAIL (TI) : -SUPERFICIAL CRUSTING,-MODERATE PREPUTIAL GLAND (PG) : -INFLAMMATION, CHRONIC-ACTIVE,-SEVERE JOINT, OTHER (JT) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT LN, POPLITEAL (PP) : -HYPERPLASIA, PLASMACYTIC,-SLIGHT 2751 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 996 ANIMAL NUMBER: C92829 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 04/24/00 STUDY DAY OF DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 694.8 GRAMS DATE AND TIME OF NECROPSY: 04/24/00 10:52 PROSECTOR: LAURIE J. SCHULLER RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), SPLEEN (SP), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PARATHYROID (PT), URINARY BLADDER (UB), LN, ANT MES/PANC (AP) 2752 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 997 ANIMAL NUMBER: C92830 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/09/00 STUDY DAY OF DEATH: 690 STUDY WEEK OF DEATH: 99 TERMINAL BODY WEIGHT: 335.5 GRAMS DATE AND TIME OF NECROPSY: 03/09/00 11:50 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); EAR-RIGHT; WEEK 47; 30 X 20MM; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; EAR-RIGHT; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >RESPIRATION; IRREGULAR; VERIFIED AT NECROPSY: NOT APPLICABLE >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; OTHER; COLD TO TOUCH; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY LIVER (LI) : -MASS(ES); ALL LOBES, DIFFUSE: MULTIPLE MASSES, UP TO 12 X 12 X 10 MM, TAN, RED, AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (HEPATIC) NOT IDENTIFIED -ADHESION(S); LEFT LATERAL, LEFT MEDIAN, AND RIGHT MEDIAN LOBES TO EACH OTHER: MULTIPLE GREY FIBROUS ADHESIONS; COLLECTED INTACT HISTOPATHOLOGY EYE (EY) : -DYSTROPHY, CORNEAL IN EARLY STAGES, CONSISTS PRIMARILY OF FOCAL MINERALIZATION,-MINIMAL LIVER (LI) : -DEGENERATION, CYSTIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL -INFILTRATE, MACROPHAGE, PIGMENTED,SLIGHT -N-SARCOMA,-NEOPLASM PRESENT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL -ATROPHY, ACINAR,-MINIMAL ADRENAL, CORTEX (AC) : -HYPERPLASIA, CORTICAL,-MINIMAL -PIGMENT,-MINIMAL 2753 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 998 ANIMAL NUMBER: C92830 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/09/00 STUDY DAY OF DEATH: 690 STUDY WEEK OF DEATH: 99 TERMINAL BODY WEIGHT: 335.5 GRAMS DATE AND TIME OF NECROPSY: 03/09/00 11:50 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY THYMUS (TH) : -MISSING/NOT IDENTIFIED; NOT POSITIVELY IDENTIFIED, POSSIBLE REMNANTS COLLECTED LUNG (LU) : -MASS(ES); ATTACHED TO ALL LOBES, AND PERICARDIUM: MULTIPLE MASSES, UP TO 25 X 15 X 10 MM, RED, TAN, SOFT, AND LOBULATED; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (MEDIASTINAL) NOT IDENTIFIED BONE, FEMUR (FE) : -N-SARCOMA,-NEOPLASM PRESENT MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,-MODERATE -HYPERPLASIA, ERYTHROID, MARROW,MODERATE MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,-MODERATE -HYPERPLASIA, ERYTHROID, MARROW,MODERATE BONE, STERNUM (SB) : -N-SARCOMA,-NEOPLASM PRESENT THYMUS (TH) : >SECTION EXAMINED; TISSUE NOT PRESENT LUNG (LU) : -N-SARCOMA,-NEOPLASM PRESENT HEART (HT) : -CARDIOMYOPATHY,-MINIMAL -N-SARCOMA,-NEOPLASM PRESENT PITUITARY (PI) : -CYST,-PRESENT 2754 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 999 ANIMAL NUMBER: C92830 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/09/00 STUDY DAY OF DEATH: 690 STUDY WEEK OF DEATH: 99 TERMINAL BODY WEIGHT: 335.5 GRAMS DATE AND TIME OF NECROPSY: 03/09/00 11:50 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -CYST(S); LEFT POSTERIOR ASPECT: SINGLE CLEAR FLUID FILLED CYST, 3 MM IN DIAMETER PROSTATE (PR) : -NODULAR; PROXIMAL SURROUNDING URETHRA: GREEN AND FIRM PINNA (PN) : -MASS(ES); INVOLVING ENTIRE RIGHT PINNA: SINGLE MASS, 30 X 30 X 17 MM, BLACK, DRY, AND FIRM; CUT SURFACE BLACK, RED, TAN, AND FIRM; COLLECTED; REGIONAL LN (MANDIBULAR) NORMAL (CAP 22) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL UPPER INCISORS -ANIMAL THIN PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT SEMINAL VESICLES (SV) : -SECRETION, DECREASED,-PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, SKIN/MUSCLE/SUBCUTIS,PRESENT LN, MANDIBULAR (MN) : -HEMORRHAGE,-PRESENT -I-SARCOMA,-NEOPLASM PRESENT PINNA (PN) : -M-SARCOMA,-NEOPLASM PRESENT 2755 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1000 ANIMAL NUMBER: C92830 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/09/00 STUDY DAY OF DEATH: 690 STUDY WEEK OF DEATH: 99 TERMINAL BODY WEIGHT: 335.5 GRAMS DATE AND TIME OF NECROPSY: 03/09/00 11:50 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: BRAIN (BR), SPLEEN (SP), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) (JE), 2756 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1001 ANIMAL NUMBER: C92831 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/29/99 STUDY DAY OF DEATH: 589 STUDY WEEK OF DEATH: 85 TERMINAL BODY WEIGHT: 617.5 GRAMS DATE AND TIME OF NECROPSY: 11/29/99 15:15 PROSECTOR: JERALD W. JOHNSON RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: NOT OBSERVED >APPEARANCE; LIMITED USE; LIMBS-ALL; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; RECUMBENT; STERNAL; VERIFIED AT NECROPSY: NOT APPLICABLE >EYES; RED DISCHARGE; EYE-RIGHT; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; COLD TO TOUCH; ENTIRE BODY; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY LIVER (LI) : -DIFFUSELY LIGHT; ALL LOBES: TAN ADRENAL, CORTEX (AC) : -DIFFUSELY RED; BILATERAL: DARK RED HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-SLIGHT -NECROSIS, INDIVIDUAL HEPATOCYTE,MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, TUBULAR,-MINIMAL -HYPERPLASIA, TRANSITIONAL CELL,MINIMAL -ANGIECTASIS,-SLIGHT PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-MODERATE -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT -HYPERTROPHY, CORTICAL CELL,-SLIGHT -PIGMENT,-MINIMAL 2757 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1002 ANIMAL NUMBER: C92831 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/29/99 STUDY DAY OF DEATH: 589 STUDY WEEK OF DEATH: 85 TERMINAL BODY WEIGHT: 617.5 GRAMS DATE AND TIME OF NECROPSY: 11/29/99 15:15 PROSECTOR: JERALD W. JOHNSON RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA, PROXIMAL TO PYLORIC SPHINCTER: MULTIPLE DARK RED PINPOINT FOCI DUODENUM (DU) : -RED FOCUS(I)/AREA(S); SEROSAL SURFACE: MULTIPLE DARK RED AREAS, UP TO 2 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION ADRENAL, MEDULLA (MA) : -HYPERPLASIA, MEDULLARY,-MINIMAL MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,-SLIGHT MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,-MODERATE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATE HEART (HT) : -CARDIOMYOPATHY,-MINIMAL MUSCLE, SKELETAL (SM) : -ATROPHY,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : >UNREMARKABLE DUODENUM (DU) : -INFLAMMATION, CHRONIC-ACTIVE,MODERATELY SEVERE -ULCERATION,-MODERATE COLON (CO) : -PARASITISM,-PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT 2758 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1003 ANIMAL NUMBER: C92831 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/29/99 STUDY DAY OF DEATH: 589 STUDY WEEK OF DEATH: 85 TERMINAL BODY WEIGHT: 617.5 GRAMS DATE AND TIME OF NECROPSY: 11/29/99 15:15 PROSECTOR: JERALD W. JOHNSON RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -TEETH MISSING/BROKEN/WORN; BILATERAL UPPER INCISORS: BROKEN OFF AT BASE -DISCHARGE-OCULAR; RIGHT: DARK RED, DRY MATERIAL CAUSE OF DEATH (CD) : -INFLAMMATION / INFECTION,-PRESENT 2759 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1004 ANIMAL NUMBER: C92831 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/29/99 STUDY DAY OF DEATH: 589 STUDY WEEK OF DEATH: 85 TERMINAL BODY WEIGHT: 617.5 GRAMS DATE AND TIME OF NECROPSY: 11/29/99 15:15 PROSECTOR: JERALD W. JOHNSON RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2760 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1005 ANIMAL NUMBER: C92832 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 08/12/99 STUDY DAY OF DEATH: 480 STUDY WEEK OF DEATH: 69 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 08/12/99 10:12 PROSECTOR: DENNIS HOFFMAN RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); SINGLE MASS, 5 X 6 X 9 MM SOFT, RED, DARK RED, AND TAN HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MINIMAL THYMUS (TH) : -HEMORRHAGE,-PRESENT -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),SLIGHT THYROID (TY) : >AUTOLYTIC PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL 2761 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1006 ANIMAL NUMBER: C92832 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 08/12/99 STUDY DAY OF DEATH: 480 STUDY WEEK OF DEATH: 69 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 08/12/99 10:12 PROSECTOR: DENNIS HOFFMAN RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL: DARK RED (CAP 21) ILEUM (IL) : >AUTOLYTIC CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT LN, MANDIBULAR (MN) : -HEMORRHAGE,-PRESENT 2762 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1007 ANIMAL NUMBER: C92832 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 08/12/99 STUDY DAY OF DEATH: 480 STUDY WEEK OF DEATH: 69 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 08/12/99 10:12 PROSECTOR: DENNIS HOFFMAN RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUm /s KULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG), GENERAL COMMENT (GC) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), CECUM (CE), COLON RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) (CO), 2763 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1008 ANIMAL NUMBER: C92833 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 04/24/00 STUDY DAY OF DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 823.8 GRAMS DATE AND TIME OF NECROPSY: 04/24/00 10:26 PROSECTOR: JODI THIEREN RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; SWOLLEN; LIMB-HIND-RIGHT; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; SWOLLEN; PAW-HIND-RIGHT; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -NECROSIS, INDIVIDUAL HEPATOCYTE,MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SLIGHT PANCREAS (PA) : -ATROPHY, ACINAR,-SLIGHT -HYPERTROPHY / BASOPHILIA, ACINAR CELL,MINIMAL TESTIS (TE) : -HYPERPLASIA, INTERSTITIAL CELL (LEYDIG CELL),-SLIGHT THYROID (TY) : -DILATATION / CYST, FOLLICULAR,-PRESENT -B-ADENOMA, FOLLICULAR CELL,-NEOPLASM PRESENT URINARY BLADDER (UB) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2764 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1009 ANIMAL NUMBER: C92833 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 04/24/00 STUDY DAY OF DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 823.8 GRAMS DATE AND TIME OF NECROPSY: 04/24/00 10:26 PROSECTOR: JODI THIEREN RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY LN, LUMBAR/ILIAC (LM) : -LARGE; LEFT: 13 X 8 X 8 MM (RIGHT IS 7 X 3 X 1 MM); LEFT APPEARS CYSTIC (CAP 23) JOINT, OTHER (JT) : -LARGE; RIGHT TARSAL JOINT: 14 MM IN DIAMETER (LEFT IS 10 MM IN DIAMETER); COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -ANIMAL OBESE LN, LUMBAR/ILIAC (LM) : -LYMPHANGIECTASIS,-PRESENT JOINT, OTHER (JT) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE 2765 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1010 ANIMAL NUMBER: C92833 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 04/24/00 STUDY DAY OF DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 823.8 GRAMS DATE AND TIME OF NECROPSY: 04/24/00 10:26 PROSECTOR: JODI THIEREN RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PARATHYROID (PT) 2766 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1011 ANIMAL NUMBER: C92834 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 04/24/00 STUDY DAY OF DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 662.6 GRAMS DATE AND TIME OF NECROPSY: 04/24/00 8:10 PROSECTOR: JODI THIEREN RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >WART LIKE LESION(S); SHOULDER-RIGHT; WEEK 95; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE DARK RED AREAS, UP TO 3 MM IN DIAMETER; COLLECTED ON ROUTINE SECTIONS PITUITARY (PI) : -DARK FOCUS(I)/AREA(S); RIGHT ANTERIOR ASPECT: SINGLE BLACK FOCUS, 1 MM IN DIAMETER HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MODERATELY SEVERE -HYPERPLASIA, BILE DUCT,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MODERATE PANCREAS (PA) : -INFILTRATION, FAT, INTERSTITIAL,SLIGHT LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT -B-ADENOMA, FOLLICULAR CELL,-NEOPLASM PRESENT PARATHYROID (PT) : -HYPERPLASIA,-MODERATE PITUITARY (PI) : -HYPERPLASIA,-MODERATE 2767 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1012 ANIMAL NUMBER: C92834 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 04/24/00 STUDY DAY OF DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 662.6 GRAMS DATE AND TIME OF NECROPSY: 04/24/00 8:10 PROSECTOR: JODI THIEREN RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY SKIN (SK) : -CRUSTED AREA(S); RIGHT SHOULDER: SINGLE DRY, TAN, RAISED, AND CRUSTED AREA, 17 X 10 X 6 MM (CAP 23) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN SKIN (SK) : -B-KERATOACANTHOMA,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2768 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1013 ANIMAL NUMBER: C92834 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 04/24/00 STUDY DAY OF DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 662.6 GRAMS DATE AND TIME OF NECROPSY: 04/24/00 8:10 PROSECTOR: JODI THIEREN RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: TESTIS (TE), URINARY BLADDER (UB) 2769 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1014 ANIMAL NUMBER: C92835 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/23/99 STUDY DAY OF DEATH: 522 STUDY WEEK OF DEATH: 75 TERMINAL BODY WEIGHT: 588.6 GRAMS DATE AND TIME OF NECROPSY: 09/23/99 8:05 PROSECTOR: JARED FISHEL RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >EYES; RED DISCHARGE; EYES; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; COLD TO TOUCH; ENTIRE BODY; VERIFIED AT NECROPSY: NOT APPLICABLE >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >RESPIRATION; LABORED; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; RECUMBENT; DORSAL; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); SINGLE MASS, 10 MM IN DIAMETER, RED, DARK RED, TAN, AND FIRM GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DRY RED MATERIAL HISTOPATHOLOGY BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL HEART (HT) : -CARDIOMYOPATHY,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 2770 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1015 ANIMAL NUMBER: C92835 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/23/99 STUDY DAY OF DEATH: 522 STUDY WEEK OF DEATH: 75 TERMINAL BODY WEIGHT: 588.6 GRAMS DATE AND TIME OF NECROPSY: 09/23/99 8:05 PROSECTOR: JARED FISHEL RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) (TH), (UB), 2771 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1016 ANIMAL NUMBER: C92836 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 01/10/00 STUDY DAY OF DEATH: 631 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 01/10/00 11:30 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; PALE; ENTIRE BODY; VERIFIED AT NECROPSY: NOT APPLICABLE >RESPIRATION; LABORED; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY LIVER (LI) : -DIFFUSELY LIGHT; ALL LOBES: TAN KIDNEY (KD) : -MASS(ES); INVOLVING ENTIRE RIGHT KIDNEY AND RIGHT ADRENAL: SINGLE MASS, 65 X 50 X 50 MM, RED, DARK RED, AND SOFT; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (RENAL) NOT IDENTIFIED SPLEEN (SP) : -LARGE; 50 X 17 X 15 MM ADRENAL, CORTEX (AC) : -MISSING/NOT IDENTIFIED; RIGHT: NOT IDENTIFIED DUE TO MASS HISTOPATHOLOGY LIVER (LI) : -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL -NECROSIS, COAGULATIVE,-MODERATE -HEMATOPOIESIS, EXTRAMEDULLARY,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SLIGHT -M-LIPOSARCOMA,-NEOPLASM PRESENT SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SEVERE PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL ADRENAL, CORTEX (AC) : -UNILATERALLY EXAMINED,-PRESENT -HYPERTROPHY, CORTICAL CELL,-MINIMAL -PIGMENT,-MINIMAL ADRENAL, MEDULLA (MA) : -UNILATERALLY EXAMINED,-PRESENT 2772 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1017 ANIMAL NUMBER: C92836 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 01/10/00 STUDY DAY OF DEATH: 631 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 01/10/00 11:30 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: MULTIPLE DARK BROWN AREAS, UP TO 2 MM IN DIAMETER GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; DRY -ANIMAL OBESE YELLOW AND MARROW, FEMUR (FM) : -HYPERPLASIA, ERYTHROID, MARROW,MODERATELY SEVERE MARROW, STERNUM (SE) : -HYPERPLASIA, ERYTHROID, MARROW,MODERATE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),SLIGHT LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL HEART (HT) : -CARDIOMYOPATHY,-MODERATE STOMACH, GL (ST) : >UNREMARKABLE STOMACH, NONGL (SU) : -ACANTHOSIS / HYPERKERATOSIS,-SLIGHT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE CAUSE OF DEATH (CD) : -NEOPLASM, KIDNEY,-PRESENT 2773 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1018 ANIMAL NUMBER: C92836 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 01/10/00 STUDY DAY OF DEATH: 631 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 01/10/00 11:30 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2774 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1019 ANIMAL NUMBER: C92837 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/12/99 STUDY DAY OF DEATH: 449 STUDY WEEK OF DEATH: 65 TERMINAL BODY WEIGHT: 461.4 GRAMS DATE AND TIME OF NECROPSY: 07/12/99 9:49 PROSECTOR: KEVIN BILLINGS RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; ROUGH HAIR COAT; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; THIN; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) KIDNEY (KD) : -GRANULAR MATERIAL; RIGHT, WITHIN PELVIS: MULTIPLE TAN PINPOINT GRANULES HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SLIGHT -MINERALIZATION, PELVIC,-MODERATE -HYPERPLASIA, TRANSITIONAL CELL,-SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-MINIMAL PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATELY SEVERE LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL HEART (HT) : -CARDIOMYOPATHY,-MINIMAL 2775 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1020 ANIMAL NUMBER: C92837 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/12/99 STUDY DAY OF DEATH: 449 STUDY WEEK OF DEATH: 65 TERMINAL BODY WEIGHT: 461.4 GRAMS DATE AND TIME OF NECROPSY: 07/12/99 9:49 PROSECTOR: KEVIN BILLINGS RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); SINGLE MASS, 10 X 10 X 8 MM, DARK RED, TAN, AND SOFT STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA: MULTIPLE TAN, ERODED AREAS, UP TO 3 MM IN DIAMETER -RED FOCUS(I)/AREA(S); SEROSAL SURFACE: SINGLE DARK RED AREA, 4 MM IN DIAMETER GENERAL COMMENT (GC) : -BLOOD FILM TAKEN PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, NONGL (SU) : -ACANTHOSIS / HYPERKERATOSIS,MODERATELY SEVERE -ULCERATION,-MODERATE -INFLAMMATION, CHRONIC-ACTIVE,MODERATELY SEVERE PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 2776 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1021 ANIMAL NUMBER: C92837 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/12/99 STUDY DAY OF DEATH: 449 STUDY WEEK OF DEATH: 65 TERMINAL BODY WEIGHT: 461.4 GRAMS DATE AND TIME OF NECROPSY: 07/12/99 9:49 PROSECTOR: KEVIN BILLINGS RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2777 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1022 ANIMAL NUMBER: C92838 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/13/99 STUDY DAY OF DEATH: 450 STUDY WEEK OF DEATH: 65 TERMINAL BODY WEIGHT: 642.1 GRAMS DATE AND TIME OF NECROPSY: 07/13/99 10:18 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >WART LIKE LESION(S); DORSAL; WEEK 64; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; BROWN HAIR COAT; SCROTUM; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; NONFORMED FECES; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY LIVER (LI) : -MOTTLED; ALL LOBES: TAN AND BROWN PITUITARY (PI) : -DIFFUSELY RED; DARK RED STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: DARK BROWN AREA, 7 X 5 MM SINGLE HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-SLIGHT -HYPERPLASIA, BILE DUCT,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, PELVIC,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL ADRENAL, CORTEX (AC) : -PIGMENT,-MINIMAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATE PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE -ULCERATION,-SLIGHT 2778 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1023 ANIMAL NUMBER: C92838 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/13/99 STUDY DAY OF DEATH: 450 STUDY WEEK OF DEATH: 65 TERMINAL BODY WEIGHT: 642.1 GRAMS DATE AND TIME OF NECROPSY: 07/13/99 10:18 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA: MULTIPLE TAN ERODED AREAS, UP TO 2 MM IN DIAMETER GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -STAINS-PERINEUM/PERIANAL; BROWN AND DRY -ANIMAL OBESE PERIANAL: STOMACH, NONGL (SU) : -ACANTHOSIS / HYPERKERATOSIS,-MODERATE -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE EPIDIDYMIDES (EP) : -HYPOSPERMIA,-PRESENT CAUSE OF DEATH (CD) : -GI TRACT, EROSION/ULCERATION/HEMORRHAGE/INFLAMMATION,-PRESENT 2779 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1024 ANIMAL NUMBER: C92838 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/13/99 STUDY DAY OF DEATH: 450 STUDY WEEK OF DEATH: 65 TERMINAL BODY WEIGHT: 642.1 GRAMS DATE AND TIME OF NECROPSY: 07/13/99 10:18 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), HEMATO NEOPLASIA (HN) 2780 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1025 ANIMAL NUMBER: C92839 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 01/02/00 STUDY DAY OF DEATH: 623 STUDY WEEK OF DEATH: 89 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 01/02/00 8:55 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >EYES; RED DISCHARGE; EYE-LEFT; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY KIDNEY (KD) : -LARGE PELVIS(ES); BILATERAL -DIFFUSELY LIGHT; TAN HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SEVERE -DILATATION, PELVIC,-MODERATE SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE PANCREAS (PA) : -INFLAMMATION, VASCULAR,-SEVERE -THROMBUS,-PRESENT ADRENAL, MEDULLA (MA) : -B-PHEOCHROMOCYTOMA,-NEOPLASM PRESENT TESTIS (TE) : -INFLAMMATION, VASCULAR,-SLIGHT MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,-MODERATE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MINIMAL -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT 2781 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1026 ANIMAL NUMBER: C92839 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 01/02/00 STUDY DAY OF DEATH: 623 STUDY WEEK OF DEATH: 89 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 01/02/00 8:55 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY STOMACH, GL (ST) : -THICKENED WALL; DIFFUSE LN, ANT MES/PANC (AP) : -LARGE; PANCREATIC: UP TO 12 X 5 X 5 MM (CAP 22) -DIFFUSELY RED; PANCREATIC: DARK RED (CAP 22) GENERAL COMMENT (GC) : -DISCHARGE-OCULAR; LEFT: DARK RED AND DRY LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,SLIGHT HEART (HT) : -CARDIOMYOPATHY,-MINIMAL -INFLAMMATION, VASCULAR,-MODERATE STOMACH, GL (ST) : -HYPERPLASIA, EPITHELIAL, GLANDULAR,MODERATE DUODENUM (DU) : -INFLAMMATION, VASCULAR,-SEVERE JEJUNUM (JE) : >AUTOLYTIC CECUM (CE) : >AUTOLYTIC COLON (CO) : -PARASITISM,-PRESENT CAUSE OF DEATH (CD) : -INFLAMMATION / INFECTION,-PRESENT LN, ANT MES/PANC (AP) : >NOT REQUIRED TO BE EXAMINED FOR ANIMAL >NOTE:>SEE PANCREAS 2782 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1027 ANIMAL NUMBER: C92839 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 01/02/00 STUDY DAY OF DEATH: 623 STUDY WEEK OF DEATH: 89 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 01/02/00 8:55 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), ADRENAL, CORTEX (AC), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), ILEUM (IL), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2783 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1028 ANIMAL NUMBER: C92840 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/03/00 STUDY DAY OF DEATH: 715 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 531.6 GRAMS DATE AND TIME OF NECROPSY: 04/03/00 14:32 PROSECTOR: NANCY DIEDRICH RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; BROWN HAIR COAT; VENTRAL-ABDOMINAL; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: NOT OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -DIFFUSELY LIGHT; ALL LOBES: TAN -RED FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE DARK RED AREAS, UP TO 3 MM IN DIAMETER; COLLECTED ON ROUTINE SECTIONS -CYST(S); VISCERAL SURFACE OF LEFT LATERAL LOBE, NEAR HILUS: SINGLE CLEAR FLUID FILLED CYST, 3 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-SEVERE -CYST, BILIARY,-PRESENT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -DILATATION, PELVIC,-SLIGHT -MINERALIZATION, PELVIC,-SLIGHT -INFLAMMATION, SUPPURATIVE, PELVIC,SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SLIGHT 2784 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1029 ANIMAL NUMBER: C92840 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/03/00 STUDY DAY OF DEATH: 715 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 531.6 GRAMS DATE AND TIME OF NECROPSY: 04/03/00 14:32 PROSECTOR: NANCY DIEDRICH RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 12 X 10 X 8 MM, RED, GRAY, TAN AND SOFT STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: MULTIPLE DARK BROWN AREAS, UP TO 2 X 1 MM STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA: MULTIPLE RED AND TAN ERODED AREAS, UP TO 5 X 2 MM -THICKENED WALL; DIFFUSE PROSTATE (PR) : -NODULAR; PROXIMAL, SURROUNDING URETHRA: GREEN AND FIRM PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,MODERATELY SEVERE ADRENAL, CORTEX (AC) : -PIGMENT,-MINIMAL THYMUS (TH) : >SECTION EXAMINED; TISSUE NOT PRESENT HEART (HT) : -CARDIOMYOPATHY,-SLIGHT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT -ULCERATION,-MINIMAL STOMACH, NONGL (SU) : -ACANTHOSIS / HYPERKERATOSIS,MODERATELY SEVERE -ULCERATION,-MODERATE -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE URINARY BLADDER (UB) : -HYPERPLASIA, TRANSITIONAL CELL,-SLIGHT -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SEVERE SEMINAL VESICLES (SV) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE 2785 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1030 ANIMAL NUMBER: C92840 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/03/00 STUDY DAY OF DEATH: 715 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 531.6 GRAMS DATE AND TIME OF NECROPSY: 04/03/00 14:32 PROSECTOR: NANCY DIEDRICH RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -STAINS-PERINEUM/PERIANAL; MOIST MATERIAL BROWN AND CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 2786 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1031 ANIMAL NUMBER: C92840 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/03/00 STUDY DAY OF DEATH: 715 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 531.6 GRAMS DATE AND TIME OF NECROPSY: 04/03/00 14:32 PROSECTOR: NANCY DIEDRICH RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) (SN), 2787 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1032 ANIMAL NUMBER: C92841 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 08/08/99 STUDY DAY OF DEATH: 476 STUDY WEEK OF DEATH: 68 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 08/08/99 8:16 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 8 X 7 X 7 MM, RED, DARK RED AND SOFT HISTOPATHOLOGY BRAIN (BR) : >UNREMARKABLE LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MINIMAL PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATE PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT JEJUNUM (JE) : >AUTOLYTIC ILEUM (IL) : >AUTOLYTIC CECUM (CE) : >AUTOLYTIC PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE 2788 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1033 ANIMAL NUMBER: C92841 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 08/08/99 STUDY DAY OF DEATH: 476 STUDY WEEK OF DEATH: 68 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 08/08/99 8:16 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG), GENERAL COMMENT (GC) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), KIDNEY (KD), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES HEMATO NEOPLASIA (HN) (EP), 2789 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1034 ANIMAL NUMBER: C92842 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/12/00 STUDY DAY OF DEATH: 633 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: 689.8 GRAMS DATE AND TIME OF NECROPSY: 01/12/00 7:45 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >WART LIKE LESION(S); DORSAL; WEEK 77; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; RED HAIR COAT; HEADENTIRE; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; CONVULSIONS; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY TESTIS (TE) : -SMALL; BILATERAL LEFT IS 20 X 17 X 5 MM, RIGHT IS 20 X 14 X 4 MM; BOTH ARE SOFT HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, PELVIC,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE PANCREAS (PA) : -INFILTRATION, FAT, INTERSTITIAL,SLIGHT ADRENAL, CORTEX (AC) : -PIGMENT,-MINIMAL TESTIS (TE) : -ATROPHY/DEGENERATION,-MODERATELY SEVERE MARROW, FEMUR (FM) : -HYPOCELLULAR, MARROW,-SEVERE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATELY SEVERE 2790 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1035 ANIMAL NUMBER: C92842 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/12/00 STUDY DAY OF DEATH: 633 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: 689.8 GRAMS DATE AND TIME OF NECROPSY: 01/12/00 7:45 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY LUNG (LU) : -UNCOLLAPSED; ALL LOBES SKIN (SK) : -CRUSTED AREA(S); MID DORSAL THORACIC REGION: SINGLE DARK RED, DRY, AND RAISED AREA, 10 X 8 X 6 MM (CAP 22) CAVITY, ABDOM (PC) : -MASS(ES); ATTACHED TO ADIPOSE TISSUE, CRANIAL TO RIGHT TESTIS: SINGLE MASS, 25 X 25 X 20 MM, YELLOW, TAN AND FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (LUMBAR) NORMAL (CAP 23) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL HEART (HT) : -CARDIOMYOPATHY,-MODERATE MUSCLE, SKELETAL (SM) : -ATROPHY,-SLIGHT NERVE, SCIATIC (SN) : -DEGENERATION,-SLIGHT SKIN (SK) : -B-KERATOACANTHOMA,-NEOPLASM PRESENT URINARY BLADDER (UB) : -PROTEINACEOUS MATERIAL, LUMINAL,PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT EPIDIDYMIDES (EP) : -HYPOSPERMIA,-PRESENT CAUSE OF DEATH (CD) : -UNDETERMINED MICROSCOPICALLY,-PRESENT CAVITY, ABDOM (PC) : -INFLAMMATION / NECROSIS, FAT,-MODERATE 2791 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1036 ANIMAL NUMBER: C92842 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/12/00 STUDY DAY OF DEATH: 633 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: 689.8 GRAMS DATE AND TIME OF NECROPSY: 01/12/00 7:45 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -DISCHARGE-NASAL; CLEAR AND MOIST -STAINS - SKIN/HAIRCOAT; ENTIRE HEAD: RED AND MOIST THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAr /i LIAC (LM), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SEMINAL VESICLES (SV), HEMATO NEOPLASIA (HN), LN, LUMBAR/ILIAC (LM) 2792 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1037 ANIMAL NUMBER: C92843 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 10/08/99 STUDY DAY OF DEATH: 537 STUDY WEEK OF DEATH: 77 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 10/08/99 13:44 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY KIDNEY (KD) : -LARGE PELVIS(ES); BILATERAL PITUITARY (PI) : -RED FOCUS(I)/AREA(S); RIGHT ASPECT: SINGLE DARK RED AREA, 3 MM IN DIAMETER HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -DEGENERATION, CYSTIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -DILATATION, PELVIC,-SLIGHT SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MINIMAL ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-MINIMAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATELY SEVERE LUNG (LU) : -INFLAMMATION, GRANULOMATOUS,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT -ANGIECTASIS,-PRESENT ILEUM (IL) : >AUTOLYTIC 2793 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1038 ANIMAL NUMBER: C92843 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 10/08/99 STUDY DAY OF DEATH: 537 STUDY WEEK OF DEATH: 77 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 10/08/99 13:44 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -ANIMAL OBESE CECUM (CE) : >AUTOLYTIC COLON (CO) : >AUTOLYTIC EPIDIDYMIDES (EP) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL CAUSE OF DEATH (CD) : -UNDETERMINED MICROSCOPICALLY,-PRESENT 2794 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1039 ANIMAL NUMBER: C92843 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 10/08/99 STUDY DAY OF DEATH: 537 STUDY WEEK OF DEATH: 77 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 10/08/99 13:44 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), HEMATO NEOPLASIA (HN) 2795 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1040 ANIMAL NUMBER: C92844 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/19/99 STUDY DAY OF DEATH: 579 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: 473.5 GRAMS DATE AND TIME OF NECROPSY: 11/19/99 9:50 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >EYES; RED DISCHARGE; EYE-RIGHT; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -LIGHT FOCUS(I)/AREA(S); ALONG MARGIN OF RIGHT MEDIAN LOBE: SINGLE TAN, SLIGHTLY RAISED AREA, 6 X 4 X 4 MM; COLLECTED ON ROUTINE SECTION HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-SLIGHT -DEGENERATION, CYSTIC,-MODERATELY SEVERE SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-MODERATE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),SEVERE LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL HEART (HT) : -CARDIOMYOPATHY,-SLIGHT PARATHYROID (PT) : >SECTION EXAMINED; TISSUE NOT PRESENT 2796 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1041 ANIMAL NUMBER: C92844 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/19/99 STUDY DAY OF DEATH: 579 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: 473.5 GRAMS DATE AND TIME OF NECROPSY: 11/19/99 9:50 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); SINGLE MASS, 12 X 9 X 8 MM, RED, DARK RED, TAN, GRAY, AND SOFT SEMINAL VESICLES (SV) : -LARGE; BILATERAL: EACH IS 30 X 20 X 5 MM GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; RIGHT: DRY CRUSTED MATERIAL -ANIMAL THIN DARK RED AND PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL SEMINAL VESICLES (SV) : >UNREMARKABLE EPIDIDYMIDES (EP) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 2797 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1042 ANIMAL NUMBER: C92844 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/19/99 STUDY DAY OF DEATH: 579 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: 473.5 GRAMS DATE AND TIME OF NECROPSY: 11/19/99 9:50 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), KIDNEY (KD), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), SALIV GL, MANDIB STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), HEMATO NEOPLASIA (HN) (SG), 2798 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1043 ANIMAL NUMBER: C92845 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/12/00 STUDY DAY OF DEATH: 664 STUDY WEEK OF DEATH: 95 TERMINAL BODY WEIGHT: 653.2 GRAMS DATE AND TIME OF NECROPSY: 02/12/00 8:13 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; HEAD TILT; RIGHT; VERIFIED AT NECROPSY: NOT APPLICABLE >EXCRETION; NO FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; RECUMBENT; LATERAL; VERIFIED AT NECROPSY: NOT APPLICABLE >RESPIRATION; IRREGULAR; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) HISTOPATHOLOGY BRAIN (BR) : ^UNREMARKABLE LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -DEGENERATION, CYSTIC,-MINIMAL -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, PERIPORTAL,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MODERATE ADRENAL, CORTEX (AC) : -MINERALIZATION,-MINIMAL -FIBROSIS,-SLIGHT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MINIMAL HEART (HT) : -CARDIOMYOPATHY,-MINIMAL 2799 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1044 ANIMAL NUMBER: C92845 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/12/00 STUDY DAY OF DEATH: 664 STUDY WEEK OF DEATH: 95 TERMINAL BODY WEIGHT: 653.2 GRAMS DATE AND TIME OF NECROPSY: 02/12/00 8:13 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 7 X 6 X 6 MM, DARK RED, TAN AND SOFT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -STAINS-PERINEUM/PERIANAL; MOIST YELLOW AND THYROID (TY) : -HYPERPLASIA, C-CELL,-MODERATE PITUITARY (PI) : -ANGIECTASIS,-PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE EPIDIDYMIDES (EP) : -VACUOLATION, TUBULAR EPITHELIUM,SLIGHT CAUSE OF DEATH (CD) : -NEPHROPATHY,-PRESENT 2800 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1045 ANIMAL NUMBER: C92845 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/12/00 STUDY DAY OF DEATH: 664 STUDY WEEK OF DEATH: 95 TERMINAL BODY WEIGHT: 653.2 GRAMS DATE AND TIME OF NECROPSY: 02/12/00 8:13 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), HEMATO NEOPLASIA (HN) 2801 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1046 ANIMAL NUMBER: C92846 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 04/09/00 STUDY DAY OF DEATH: 721 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 04/09/00 7:25 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED >WART LIKE LESION(S); DORSAL; WEEK 80; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -MOTTLED; ALL LOBES: RED AND TAN KIDNEY (KD) : -CYST(S); BILATERAL CORTICAL SURFACES, EXTENDING TO MEDULLA: MULTIPLE CLEAR FLUID FILLED CYSTS, UP TO 3 MM IN DIAMETER -LARGE PELVIS(ES); BILATERAL -ROUGH SURFACE, DIFFUSE; BILATERAL -LARGE; BILATERAL: EACH IS 32 X 22 X 14 MM -DIFFUSELY LIGHT; BILATERAL: TAN PANCREAS (PA) : -LARGE VESSELS; DIFFUSE: UP TO 6 MM IN DIAMETER (CAP 24) HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-SLIGHT -DEGENERATION, CYSTIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-SLIGHT -FIBROSIS, PERIBILIARY,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SEVERE -DILATATION, PELVIC,-SLIGHT -CYST,-PRESENT -B-PAPILLOMA, TRANSITIONAL CELL,NEOPLASM PRESENT SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE PANCREAS (PA) : -INFLAMMATION, VASCULAR,-SLIGHT -THROMBUS,-PRESENT ADRENAL, CORTEX (AC) : -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL 2802 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1047 ANIMAL NUMBER: C92846 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 04/09/00 STUDY DAY OF DEATH: 721 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 04/09/00 7:25 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY LUNG (LU) : -UNCOLLAPSED; ALL LOBES HEART (HT) : -LARGE; 30 X 20 X 14 MM ADRENAL, CORTEX (AC) : -HYPERTROPHY, CORTICAL CELL,-MODERATELY SEVERE -PIGMENT,-SLIGHT ADRENAL, MEDULLA (MA) : -HYPERPLASIA, MEDULLARY,-MODERATE LN, MESENTERIC (MS) : -HYPERPLASIA, LYMPHOPLASMACYTIC,MODERATELY SEVERE TESTIS (TE) : -ATROPHY/DEGENERATION,-SLIGHT -INFLAMMATION, VASCULAR,-SLIGHT MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,MODERATELY SEVERE MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,-SLIGHT -MYELOFIBROSIS,-MINIMAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATELY SEVERE LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL HEART (HT) : -CARDIOMYOPATHY, -SLIGHT THYROID (TY) : -DILATATION / CYST, FOLLICULAR,-PRESENT 2803 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1048 ANIMAL NUMBER: C92846 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 04/09/00 STUDY DAY OF DEATH: 721 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 04/09/00 7:25 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY THYROID (TY) : -LARGE; BILATERAL: EACH IS 7 X 3 X 2 MM PARATHYROID (PT) : -LARGE; BILATERAL: EACH IS 2 MM IN DIAMETER PITUITARY (PI) : -LARGE; 4 X 4 X 3 MM; APPEARS SWOLLEN STOMACH, GL (ST) : -THICKENED WALL; MUCOSA SKIN (SK) : -CRUSTED AREA(S); RIGHT DORSAL SHOULDER REGION: SINGLE TAN, DRY AND CAULIFLOWER- LIKE CRUSTED AREA, 25 X 20 MM (CAP 30) PROSTATE (PR) : -NODULAR; PROXIMAL, TISSUE SURROUNDING URETHRA: GREEN -LARGE; 3 3 X 2 3 X 9 MM SEMINAL VESICLES (SV) : -LARGE; BILATERAL: EACH IS 43 X 33 X 22 MM PARATHYROID (PT) : -HYPERPLASIA,-MODERATELY SEVERE PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : -HYPERPLASIA, EPITHELIAL, GLANDULAR,MODERATE -MINERALIZATION, GLANDULAR MUCOSA,SLIGHT ILEUM (IL) : >AUTOLYTIC SKIN (SK) : -B-KERATOACANTHOMA,-NEOPLASM PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE SEMINAL VESICLES (SV) : >UNREMARKABLE EPIDIDYMIDES (EP) : -IMMATURE SPERM FORMS,-MODERATE 2804 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1049 ANIMAL NUMBER: C92846 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 04/09/00 STUDY DAY OF DEATH: 721 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 04/09/00 7:25 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY LN, MEDIASTINAL (ML) : -LARGE; UP TO 5 X 3 X 2 MM (CAP 22) -DIFFUSELY RED LN, RENAL (RL) : -LARGE; RIGHT: 4 X 3 X 3 MM (LEFT, SEE CYST COMMENT) (CAP 26) -CYST(S); INVOLVING ENTIRE LEFT: SINGLE CLEAR FLUID FILLED CYST, 6 X 3 X 3 MM (CAP 26) LN, LUMBAR/ILIAC (LM) : -CYST(S); ATTACHED TO CRANIAL END OF LEFT: SINGLE CLEAR FLUID FILLED CYST, 3 X 3 X 2 MM (RIGHT IS NORMAL) (CAP 29) CAVITY, ABDOM (PC) : -LARGE VESSELS; MESENTERY, DIFFUSE: UP TO 6 MM IN DIAMETER (CAP 25) AORTA (AO) : -LARGE; DIFFUSE: UP TO 5 MM IN DIAMETER (CAP 23) -FIRM; DIFFUSE URETER (UE) : -LARGE; BILATERAL: EACH IS UP TO 2 MM IN DIAMETER (CAP 27) VAS DEFERENS (VS) : -LARGE; BILATERAL: EACH IS UP TO 3 MM IN DIAMETER (CAP 28) CAUSE OF DEATH (CD) : -NEPHROPATHY,-PRESENT LN, MEDIASTINAL (ML) : -HEMORRHAGE,-PRESENT -LYMPHANGIECTASIS,-MODERATELY SEVERE LN, RENAL (RL) : -HYPERPLASIA, LYMPHOPLASMACYTIC,MODERATE -HEMORRHAGE,-PRESENT -LYMPHANGIECTASIS,-MODERATE LN, LUMBAR/ILIAC (LM) : -LYMPHANGIECTASIS,-PRESENT -HYPERPLASIA, LYMPHOPLASMACYTIC,MODERATELY SEVERE CAVITY, ABDOM (PC) : -INFLAMMATION, VASCULAR,-MODERATE -THROMBUS,-PRESENT AORTA (AO) : >UNREMARKABLE URETER (UE) : -UNILATERALLY EXAMINED,-PRESENT VAS DEFERENS (VS) : >UNREMARKABLE 2805 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1050 ANIMAL NUMBER: C92846 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 04/09/00 STUDY DAY OF DEATH: 721 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 04/09/00 7:25 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -MALOCCLUSION; BILATERAL, UPPER INCISORS -FLUID-ABDOMINAL CAVITY; DARK RED MATERIAL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), SPLEEN (SP), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), EPIDIDYMIDES (EP), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), HEMATO NEOPLASIA (HN) 2806 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1051 ANIMAL NUMBER: C92847 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/28/00 STUDY DAY OF DEATH: 680 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: 579.0 GRAMS DATE AND TIME OF NECROPSY: 02/28/00 15:08 PROSECTOR: JARED FISHEL RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; SORE/SCAB; PAW-HINDRIGHT; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >EYES; RED DISCHARGE; EYE-LEFT; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: NOT OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) KIDNEY (KD) : -ROUGH SURFACE, DIFFUSE; LEFT -SMALL; LEFT: 20 X 10 X 10 MM (RIGHT IS 25 X 17 X 15 MM) HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -NECROSIS, INDIVIDUAL HEPATOCYTE,MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MODERATE -DILATATION, PELVIC,-SLIGHT PANCREAS (PA) : -ATROPHY, ACINAR,-MINIMAL ADRENAL, CORTEX (AC) : -PIGMENT,-MINIMAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATELY SEVERE LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL HEART (HT) : -CARDIOMYOPATHY,-SLIGHT 2807 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1052 ANIMAL NUMBER: C92847 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/28/00 STUDY DAY OF DEATH: 680 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: 579.0 GRAMS DATE AND TIME OF NECROPSY: 02/28/00 15:08 PROSECTOR: JARED FISHEL RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY HEART (HT) : -LIGHT FOCUS(I)/AREA(S); EPICARDIUM OF LEFT VENTRICLE: MULTIPLE GREY AREAS, UP TO 5 X 2 MM PITUITARY (PI) : -MASS(ES); 12 X 9 X 9 MM, LOBULATED, AND SOFT STOMACH, GL (ST) : -THICKENED WALL; DIFFUSE DARK RED, FOOT/FOOTPAD (FP) : -CRUSTED AREA(S); RIGHT, HIND FOOT: SINGLE RED AND DARK RED CRUSTED AREA, 11 MM IN DIAMETER, COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DRY RED MATERIAL THYROID (TY) : -B-ADENOMA, FOLLICULAR CELL,-NEOPLASM PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : -HYPERPLASIA, EPITHELIAL, GLANDULAR,SLIGHT PROSTATE (PR) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT FOOT/FOOTPAD (FP) : -INFLAMMATION, CHRONIC-ACTIVE,MODERATELY SEVERE -ULCERATION,-MODERATE -ACANTHOSIS / HYPERKERATOSIS,-MODERATE 2808 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1053 ANIMAL NUMBER: C92847 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/28/00 STUDY DAY OF DEATH: 680 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: 579.0 GRAMS DATE AND TIME OF NECROPSY: 02/28/00 15:08 PROSECTOR: JARED FISHEL RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2809 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1054 ANIMAL NUMBER: C92848 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 04/24/00 STUDY DAY OF DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 406.9 GRAMS DATE AND TIME OF NECROPSY: 04/24/00 8:35 PROSECTOR: JAMES T. CALLEN RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; SWOLLEN; PAW-HIND-LEFT; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; LIMITED USE; PAW-HINDLEFT; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY KIDNEY (KD) : -LARGE PELVIS(ES); RIGHT PITUITARY (PI) : -RED FOCUS(I)/AREA(S); RIGHT ASPECT: SINGLE DARK RED FOCUS, 2 MM IN DIAMETER LN, LUMBAR/ILIAC (LM) : -LARGE; LEFT: 11 X 9 X 6 MM (RIGHT IS 4 X 3 X 2 MM) (CAP 23) JOINT, OTHER (JT) : -LARGE; LEFT TARSAL: 16 MM IN DIAMETER; COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -ANIMAL THIN HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -DILATATION, PELVIC,-MODERATE PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-MODERATE PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, LUMBAR/ILIAC (LM) : -LYMPHANGIECTASIS,-PRESENT JOINT, OTHER (JT) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT -FIBROSIS,-MODERATELY SEVERE -HYPERPLASIA, BONE,-SEVERE 2810 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1055 ANIMAL NUMBER: C92848 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 04/24/00 STUDY DAY OF DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 406.9 GRAMS DATE AND TIME OF NECROPSY: 04/24/00 8:35 PROSECTOR: JAMES T. CALLEN RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: TESTIS (TE), LUNG (LU), THYROID (TY), PARATHYROID (PT), URINARY BLADDER (UB) 2811 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1056 ANIMAL NUMBER: C92849 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/16/99 STUDY DAY OF DEATH: 484 STUDY WEEK OF DEATH: 70 TERMINAL BODY WEIGHT: 476.9 GRAMS DATE AND TIME OF NECROPSY: 08/16/99 14:45 PROSECTOR: TONY HANFELD RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >EXCRETION; NO FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >EYES; SQUINTED; PERI-ORBITAL; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; COLD TO TOUCH; ENTIRE BODY; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; MODERATE; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) KIDNEY (KD) : -GRANULAR MATERIAL; RIGHT, WITHIN PELVIS: MULTIPLE TAN PINPOINT GRANULES PITUITARY (PI) : -MASS(ES); SINGLE MASS, 10 X 8 X 8 MM, TAN, RED, DARK RED, AND SOFT HISTOPATHOLOGY BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -NECROSIS, COAGULATIVE,-SLIGHT -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-SLIGHT -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL ADRENAL, CORTEX (AC) : -PIGMENT,-MINIMAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATELY SEVERE PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT 2812 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1057 ANIMAL NUMBER: C92849 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/16/99 STUDY DAY OF DEATH: 484 STUDY WEEK OF DEATH: 70 TERMINAL BODY WEIGHT: 476.9 GRAMS DATE AND TIME OF NECROPSY: 08/16/99 14:45 PROSECTOR: TONY HANFELD RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: SINGLE DARK BROWN AREA, 2 X 1 MM GENERAL COMMENT (GC) : -BLOOD FILM TAKEN STOMACH, GL (ST) : -EDEMA,-MODERATE -INFLAMMATION, ACUTE,-SLIGHT -EROSION,-MINIMAL PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 2813 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1058 ANIMAL NUMBER: C92849 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/16/99 STUDY DAY OF DEATH: 484 STUDY WEEK OF DEATH: 70 TERMINAL BODY WEIGHT: 476.9 GRAMS DATE AND TIME OF NECROPSY: 08/16/99 14:45 PROSECTOR: TONY HANFELD RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2814 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1059 ANIMAL NUMBER: C92850 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/18/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 482.8 GRAMS DATE AND TIME OF NECROPSY: 04/18/00 7:26 PROSECTOR: JODI THIEREN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY LIVER (LI) : -DIFFUSELY LIGHT; ALL LOBES: TAN SPLEEN (SP) : -MASS(ES); INVOLVING ENTIRE SPLEEN: SINGLE MASS, 55 X 33 X 33 MM, RED, DARK RED, TAN AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (SEE PANCREATIC) HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-SLIGHT -VACUOLATION, HEPATOCELLULAR, MIDZONAL / CENTRILOBULAR,-MODERATE -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-SLIGHT -DEGENERATION, CYSTIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, PERIPORTAL,-SEVERE -INFILTRATE, MACROPHAGE, PIGMENTED,SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE -M-HEMANGIOSARCOMA,-NEOPLASM PRESENT PANCREAS (PA) : -M-CARCINOMA, ISLET CELL,-NEOPLASM PRESENT -INFLAMMATION, VASCULAR,-MODERATE 2815 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1060 ANIMAL NUMBER: C92850 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/18/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 482.8 GRAMS DATE AND TIME OF NECROPSY: 04/18/00 7:26 PROSECTOR: JODI THIEREN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: MULTIPLE DARK BROWN AREAS, UP TO 1 MM IN DIAMETER LN, ANT MES/PANC (AP) : -LARGE; PANCREATIC: UP TO 7 X 5 X 5 MM (CAP 22) -DIFFUSELY RED; PANCREATIC: DARK RED ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-MINIMAL -HYPERTROPHY, CORTICAL CELL,-SLIGHT -PIGMENT,-SLIGHT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATELY SEVERE HEART (HT) : -CARDIOMYOPATHY,-MODERATE MUSCLE, SKELETAL (SM) : -ATROPHY,-SLIGHT PITUITARY (PI) : -HYPERPLASIA,-MINIMAL -CYST,-PRESENT STOMACH, GL (ST) : -EROSION,-MINIMAL PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE SEMINAL VESICLES (SV) : -SECRETION, DECREASED,-PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, SPLEEN,-PRESENT LN, ANT MES/PANC (AP) : >NOT REQUIRED TO BE EXAMINED FOR ANIMAL >NOTE:>SEE PANCREAS FOR DIAGNOSIS OF MASS. LYMPH NODE NOT INVOLVED. 2816 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1061 ANIMAL NUMBER: C92850 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/18/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 482.8 GRAMS DATE AND TIME OF NECROPSY: 04/18/00 7:26 PROSECTOR: JODI THIEREN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN -STAINS-PERINEUM/PERIANAL; YELLOW MATERIAL MOIST, 2817 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1062 ANIMAL NUMBER: C92850 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/18/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 482.8 GRAMS DATE AND TIME OF NECROPSY: 04/18/00 7:26 PROSECTOR: JODI THIEREN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUm /s KULL (CA), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2818 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1063 ANIMAL NUMBER: C92851 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 04/24/00 STUDY DAY OF DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 788.7 GRAMS DATE AND TIME OF NECROPSY: 04/24/00 9:53 PROSECTOR: LAURIE J. SCHULLER RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >WART LIKE LESION(S); VENTRAL ABDOMINAL; WEEK 102; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE DARK RED AREAS, UP TO 3 X 2 MM; COLLECTED ON ROUTINE SECTIONS SPLEEN (SP) : -ADHESION(S); PARIETAL SURFACE ATTACHED TO SURROUNDING ADIPOSE TISSUE: MULTIPLE GRAY FIBROUS ADHESIONS; COLLECTED INTACT LUNG (LU) : -LIGHT FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE TAN AREAS, UP TO 3 MM IN DIAMETER; SOME ARE FIRM HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MODERATE -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SLIGHT SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SLIGHT -FIBROSIS, CAPSULAR,-PRESENT PANCREAS (PA) : -HYPERTROPHY / BASOPHILIA, ACINAR CELL,MINIMAL LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL 2819 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1064 ANIMAL NUMBER: C92851 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 04/24/00 STUDY DAY OF DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 788.7 GRAMS DATE AND TIME OF NECROPSY: 04/24/00 9:53 PROSECTOR: LAURIE J. SCHULLER RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY THYROID (TY) : -MASS(ES); RIGHT: SINGLE MASS, 6 X 4 X 3 MM, RED, TAN AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (MEDIASTINAL) NORMAL (CAP 23) SKIN (SK) : -CRUSTED AREA(S); MID ABDOMINAL REGION: SINGLE BROWN, TAN, DRY CRUSTED AREA, 6 MM IN DIAMETER (CAP 24) LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL; COLLECTED TAIL (TI) : -CRUSTED AREA(S); ALONG ENTIRE LENGTH: MULTIPLE DARK BROWN AREAS, UP TO 3 MM IN DIAMETER; COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN THYROID (TY) : -M-CARCINOMA, FOLLICULAR CELL,-NEOPLASM PRESENT SKIN (SK) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT -B-PAPILLOMA, SQUAMOUS CELL,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MEDIASTINAL (ML) : -HYPERPLASIA, LYMPHOPLASMACYTIC,MINIMAL LN, MANDIBULAR (MN) : -HYPERPLASIA, LYMPHOPLASMACYTIC,MODERATE -HEMORRHAGE,-PRESENT -LYMPHANGIECTASIS,-PRESENT TAIL (TI) : -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL -ACANTHOSIS / HYPERKERATOSIS,-MINIMAL 2820 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1065 ANIMAL NUMBER: C92851 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 04/24/00 STUDY DAY OF DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 788.7 GRAMS DATE AND TIME OF NECROPSY: 04/24/00 9:53 PROSECTOR: LAURIE J. SCHULLER RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), MAMMARY, MALE (MM), COAGULATING GL (CG), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: TESTIS (TE), PARATHYROID (PT), URINARY BLADDER (UB) 2821 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1066 ANIMAL NUMBER: C92852 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/07/99 STUDY DAY OF DEATH: 475 STUDY WEEK OF DEATH: 68 TERMINAL BODY WEIGHT: 544.2 GRAMS DATE AND TIME OF NECROPSY: 08/07/99 8:05 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; THIN; VERIFIED AT NECROPSY: NOT OBSERVED >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; HUNCHED; VERIFIED AT NECROPSY: NOT APPLICABLE >RESPIRATION; IRREGULAR; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -RED FOCUS(I)/AREA(S); DIAPHRAGMATIC SURFACE OF LEFT LATERAL LOBE: MULTIPLE DARK RED AREAS, UP TO 2 X 1 MM; COLLECTED ON ROUTINE SECTION HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -DEGENERATION, CYSTIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT ADRENAL, CORTEX (AC) : -PIGMENT,-MINIMAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),SEVERE LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL 2822 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1067 ANIMAL NUMBER: C92852 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/07/99 STUDY DAY OF DEATH: 475 STUDY WEEK OF DEATH: 68 TERMINAL BODY WEIGHT: 544.2 GRAMS DATE AND TIME OF NECROPSY: 08/07/99 8:05 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 8 X 7 X 7 MM, RED, DARK RED, AND SOFT STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA: MULTIPLE DARK BROWN AND BLACK ERODED AREAS, UP TO 6 X 4 MM -THICKENED WALL; DIFFUSE GENERAL COMMENT (GC) : -BLOOD FILM TAKEN PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL STOMACH, NONGL (SU) : -ACANTHOSIS / HYPERKERATOSIS,-MODERATE -ULCERATION,-MODERATE -INFLAMMATION, CHRONIC-ACTIVE,MODERATELY SEVERE PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 2823 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1068 ANIMAL NUMBER: C92852 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/07/99 STUDY DAY OF DEATH: 475 STUDY WEEK OF DEATH: 68 TERMINAL BODY WEIGHT: 544.2 GRAMS DATE AND TIME OF NECROPSY: 08/07/99 8:05 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) (MS), THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) (SM), 2824 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1069 ANIMAL NUMBER: C92853 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 04/24/00 STUDY DAY OF DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 691.9 GRAMS DATE AND TIME OF NECROPSY: 04/24/00 8:12 PROSECTOR: JAMES T. CALLEN RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 12 X 8 X 6 MM, RED, DARK RED, TAN AND FIRM STOMACH, GL (ST) : -EROSION/ULCERATION; MUCOSA: SINGLE DARK RED ERODED AREA, 5 MM IN DIAMETER HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL -VACUOLATION, HEPATOCELLULAR,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL PANCREAS (PA) : -HYPERPLASIA, ISLET CELLS,-SLIGHT LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL THYROID (TY) : -B-ADENOMA, C-CELL,- 2 NEOPLASMS PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : -INFLAMMATION, CHRONIC-ACTIVE,MODERATELY SEVERE -ULCERATION,-MODERATE 2825 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1070 ANIMAL NUMBER: C92853 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 04/24/00 STUDY DAY OF DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 691.9 GRAMS DATE AND TIME OF NECROPSY: 04/24/00 8:12 PROSECTOR: JAMES T. CALLEN RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN STOMACH, GL (ST) : -HYPERPLASIA, EPITHELIAL, GLANDULAR,MODERATE CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUm /s KULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: TESTIS (TE), PARATHYROID (PT), URINARY BLADDER (UB) 2826 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1071 ANIMAL NUMBER: C92854 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/24/00 STUDY DAY OF DEATH: 705 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/24/00 7:29 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY HISTOPATHOLOGY LIVER (LI) : -DEGENERATION, CYSTIC,-MINIMAL -NECROSIS, COAGULATIVE,-SLIGHT -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATELY SEVERE PANCREAS (PA) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT ADRENAL, CORTEX (AC) : -HYPERTROPHY, CORTICAL CELL,-MINIMAL TESTIS (TE) : -B-INTERSTITIAL CELL TUMOR,-NEOPLASM PRESENT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATELY SEVERE 2827 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1072 ANIMAL NUMBER: C92854 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/24/00 STUDY DAY OF DEATH: 705 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/24/00 7:29 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: TWO DARK BROWN AREAS, EACH IS 2 X 1 MM LUNG (LU) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT HEART (HT) : -CARDIOMYOPATHY,-MODERATE THYROID (TY) : -B-ADENOMA, FOLLICULAR CELL,-NEOPLASM PRESENT PITUITARY (PI) : -HYPERPLASIA,-MINIMAL -CYST,-PRESENT STOMACH, GL (ST) : >UNREMARKABLE JEJUNUM (JE) : >AUTOLYTIC ILEUM (IL) : >AUTOLYTIC CECUM (CE) : >AUTOLYTIC COLON (CO) : >AUTOLYTIC RECTUM (RE) : >AUTOLYTIC SKIN (SK) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT 2828 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1073 ANIMAL NUMBER: C92854 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/24/00 STUDY DAY OF DEATH: 705 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/24/00 7:29 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -DISCHARGE-OCULAR; BILATERAL: DARK RED, DRY MATERIAL EPIDIDYMIDES (EP) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT HEMATO NEOPLASIA (HN) : -M-SARCOMA, HISTIOCYTIC,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, HEMATOPOIETIC,-PRESENT 2829 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1074 ANIMAL NUMBER: C92854 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/24/00 STUDY DAY OF DEATH: 705 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/24/00 7:29 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL DUODENUM (DU), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV) (SU), 2830 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1075 ANIMAL NUMBER: C92855 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/16/99 STUDY DAY OF DEATH: 484 STUDY WEEK OF DEATH: 70 TERMINAL BODY WEIGHT: 448.1 GRAMS DATE AND TIME OF NECROPSY: 08/16/99 9:46 PROSECTOR: TONY HANFELD RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: NOT OBSERVED >SKIN & PELAGE; ROUGH HAIR COAT; VERIFIED AT NECROPSY: NOT APPLICABLE >WART LIKE LESION(S); VENTRAL-ABDOMINALLEFT; WEEK 69; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; HUNCHED; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) TESTIS (TE) : -CONTAINS FLUID; BILATERAL, BENEATH TUNICA: CLEAR FLUID HISTOPATHOLOGY EYE (EY) : -DYSTROPHY, CORNEAL IN EARLY STAGES, CONSISTS PRIMARILY OF FOCAL MINERALIZATION,-MINIMAL BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL TESTIS (TE) : -ATROPHY/DEGENERATION,-SEVERE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATE LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL 2831 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1076 ANIMAL NUMBER: C92855 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/16/99 STUDY DAY OF DEATH: 484 STUDY WEEK OF DEATH: 70 TERMINAL BODY WEIGHT: 448.1 GRAMS DATE AND TIME OF NECROPSY: 08/16/99 9:46 PROSECTOR: TONY HANFELD RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); SINGLE MASS, 10 X 7 X 5 MM, RED, DARK RED, TAN, SOFT AND LOBULATED SKIN (SK) : -CRUSTED AREA(S); VENTRAL ABDOMINAL REGION: SINGLE TAN AND RAISED AREA, 3 X 3 X 2 MM (CAP 21); COLLECTED; POSSIBLY CONSISTENT WITH WART-LIKE LESION AS NOTED IN CLINICAL OBSERVATIONS CAVITY, ABDOM (PC) : -MASS(ES); ATTACHED TO ADIPOSE TISSUE NEAR LEFT EPIDIDYMIS: SINGLE MASS, 11 X 4 X 3 MM, TAN, YELLOW AND FIRM; SAME ON CUT SURFACE (CAP 22); REGIONAL LN (MESENTERIC) NORMAL GENERAL COMMENT (GC) : -BLOOD FILM TAKEN PARATHYROID (PT) : >SECTION EXAMINED; TISSUE NOT PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-SLIGHT RECTUM (RE) : -PARASITISM,-PRESENT SKIN (SK) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT -B-KERATOACANTHOMA,-NEOPLASM PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT CAVITY, ABDOM (PC) : -INFLAMMATION / NECROSIS, FAT,-MODERATE 2832 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1077 ANIMAL NUMBER: C92855 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/16/99 STUDY DAY OF DEATH: 484 STUDY WEEK OF DEATH: 70 TERMINAL BODY WEIGHT: 448.1 GRAMS DATE AND TIME OF NECROPSY: 08/16/99 9:46 PROSECTOR: TONY HANFELD RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -TEETH MISSING/BROKEN/WORN; BILATERAL, UPPER INCISORS: BROKEN OFF NEAR BASE -DISCHARGE-OCULAR; BILATERAL: RED AND DRY 2833 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1078 ANIMAL NUMBER: C92855 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/16/99 STUDY DAY OF DEATH: 484 STUDY WEEK OF DEATH: 70 TERMINAL BODY WEIGHT: 448.1 GRAMS DATE AND TIME OF NECROPSY: 08/16/99 9:46 PROSECTOR: TONY HANFELD RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LIVER (LI), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) (FM), (SN), 2834 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1079 ANIMAL NUMBER: C92856 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 04/24/00 STUDY DAY OF DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 606.4 GRAMS DATE AND TIME OF NECROPSY: 04/24/00 8:37 PROSECTOR: JOHN S. HALFORD RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; BENT TAIL; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); INGUINAL REGION-LEFT; WEEK 99; VERIFIED AT NECROPSY: NOT OBSERVED OBSERVATIONS NECROPSY KIDNEY (KD) : -CYST(S); LEFT CORTICAL SURFACE: SINGLE CLEAR FLUID FILLED CYST, 3 MM IN DIAMETER -ROUGH SURFACE, DIFFUSE; BILATERAL -DIFFUSELY LIGHT; BILATERAL: TAN PANCREAS (PA) : -LARGE VESSELS; UP TO 3 MM IN DIAMETER (CAP 24) LUNG (LU) : -MOTTLED; ALL LOBES: RED AND TAN HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-SLIGHT -HYPERPLASIA, BILE DUCT,-MINIMAL -FIBROSIS, PERIBILIARY,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, PERIPORTAL,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MODERATELY SEVERE PANCREAS (PA) : -INFLAMMATION, VASCULAR,-SEVERE LUNG (LU) : >UNREMARKABLE THYROID (TY) : -B-ADENOMA, FOLLICULAR CELL,-NEOPLASM PRESENT PARATHYROID (PT) : -HYPERPLASIA,-SEVERE 2835 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1080 ANIMAL NUMBER: C92856 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 04/24/00 STUDY DAY OF DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 606.4 GRAMS DATE AND TIME OF NECROPSY: 04/24/00 8:37 PROSECTOR: JOHN S. HALFORD RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -CYST(S); LEFT POSTERIOR ASPECT: SINGLE CLEAR FLUID FILLED CYST, 3 MM IN DIAMETER STOMACH, GL (ST) : -THICKENED WALL; DIFFUSE LN, MEDIASTINAL (ML) : -LARGE; UP TO 4 X 4 X 3 MM (CAP 22) LN, RENAL (RL) : -LARGE; BILATERAL: EACH IS UP TO 5 X 4 X 3 MM (CAP 23) -MOTTLED; BILATERAL: RED AND TAN CAVITY, ABDOM (PC) : -LARGE VESSELS; MESENTERY: UP TO 4 MM IN DIAMETER (CAP 25) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -TAIL - BENT/MISSING; 20 MM AND 40 MM FROM TIP: TWO BENT AREAS; COLLECTED -BONE MARROW SMEAR TAKEN PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : -HYPERPLASIA, EPITHELIAL, GLANDULAR,SLIGHT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MEDIASTINAL (ML) : -LYMPHANGIECTASIS,-MODERATE LN, RENAL (RL) : -INFILTRATE, MACROPHAGE, PIGMENTED,SLIGHT -LYMPHANGIECTASIS,-MODERATELY SEVERE CAVITY, ABDOM (PC) : -INFLAMMATION, VASCULAR,-MODERATE 2836 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1081 ANIMAL NUMBER: C92856 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 04/24/00 STUDY DAY OF DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 606.4 GRAMS DATE AND TIME OF NECROPSY: 04/24/00 8:37 PROSECTOR: JOHN S. HALFORD RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), SPLEEN (SP), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: TESTIS (TE), URINARY BLADDER (UB) 2837 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1082 ANIMAL NUMBER: C92857 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/16/99 STUDY DAY OF DEATH: 576 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: 570.5 GRAMS DATE AND TIME OF NECROPSY: 11/16/99 9:35 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >EXCRETION; NO FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; RECUMBENT; STERNAL; VERIFIED AT NECROPSY: NOT APPLICABLE >EYES; SQUINTED; PERI-ORBITAL; VERIFIED AT NECROPSY: NOT APPLICABLE >EYES; RED DISCHARGE; EYES; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) HISTOPATHOLOGY BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -HYPERPLASIA, BILE DUCT,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MODERATE SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-SLIGHT -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-SLIGHT -HYPERTROPHY, CORTICAL CELL,-MINIMAL 2838 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1083 ANIMAL NUMBER: C92857 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/16/99 STUDY DAY OF DEATH: 576 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: 570.5 GRAMS DATE AND TIME OF NECROPSY: 11/16/99 9:35 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); SINGLE MASS, 9 X 8 X 7 MM, RED, DARK RED, TAN AND SOFT URINARY BLADDER (UB) : -CALCULUS(I); WITHIN LUMEN: SINGLE TAN AND HARD CALCULUS, 6 MM IN DIAMETER PROSTATE (PR) : -NODULAR; PROXIMAL SURROUNDING URETHRA: GREEN AND FIRM SEMINAL VESICLES (SV) : -LARGE; BILATERAL: EACH IS 30 X 20 X 6 MM LN, LUMBAR/ILIAC (LM) : -LARGE; BILATERAL: EACH IS UP TO 12 X 7 X 4 MM (CAP 22) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DARK RED AND DRY MATERIAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),SEVERE THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL URINARY BLADDER (UB) : >UNREMARKABLE PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE SEMINAL VESICLES (SV) : -SECRETION, INCREASED,-PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT LN, LUMBAR/ILIAC (LM) : >TISSUE MISSING 2839 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1084 ANIMAL NUMBER: C92857 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/16/99 STUDY DAY OF DEATH: 576 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: 570.5 GRAMS DATE AND TIME OF NECROPSY: 11/16/99 9:35 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2840 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1085 ANIMAL NUMBER: C92858 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/21/00 STUDY DAY OF DEATH: 673 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 526.8 GRAMS DATE AND TIME OF NECROPSY: 02/21/00 14:44 PROSECTOR: DAVID SCHUETTE RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >WART LIKE LESION(S); DORSAL; WEEK 87; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); AXILLARY REGION-RIGHT; WEEK 91; 15 X 20MM; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; THIN; VERIFIED AT NECROPSY: NOT OBSERVED >RESPIRATION; AUDIBLE; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -MOTTLED; ALL LOBES: BROWN AND TAN -ROUGH SURFACE, DIFFUSE; ALL LOBES HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-SLIGHT -NECROSIS, INDIVIDUAL HEPATOCYTE,MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-MODERATELY SEVERE -HYPERPLASIA, BILE DUCT,-MODERATELY SEVERE KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SLIGHT SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-SLIGHT -INFILTRATE, MACROPHAGE, PIGMENTED,SLIGHT -HYPERPLASIA, ISLET CELLS,-SLIGHT -HYPERTROPHY / BASOPHILIA, ACINAR CELL,MINIMAL ADRENAL, CORTEX (AC) : -PIGMENT,-SLIGHT 2841 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1086 ANIMAL NUMBER: C92858 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/21/00 STUDY DAY OF DEATH: 673 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 526.8 GRAMS DATE AND TIME OF NECROPSY: 02/21/00 14:44 PROSECTOR: DAVID SCHUETTE RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 8 X 5 X 5 MM, DARK RED, TAN, GRAY, AND SOFT MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,-SLIGHT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATELY SEVERE LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL -HEMORRHAGE,-PRESENT -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL -INFLAMMATION, GRANULOMATOUS,-SLIGHT >NOTE:>SOME INFLAMMATORY FOCI CONTAIN REFRACTILE FOREIGN MATERIAL HEART (HT) : -CARDIOMYOPATHY,-MINIMAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT -B-ADENOMA, FOLLICULAR CELL,-NEOPLASM PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, NONGL (SU) : -ACANTHOSIS / HYPERKERATOSIS,-MINIMAL SKIN (SK) : -B-KERATOACANTHOMA,-NEOPLASM PRESENT -B-RHABOMYOMA,-NEOPLASM PRESENT 2842 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1087 ANIMAL NUMBER: C92858 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/21/00 STUDY DAY OF DEATH: 673 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 526.8 GRAMS DATE AND TIME OF NECROPSY: 02/21/00 14:44 PROSECTOR: DAVID SCHUETTE RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY SKIN (SK) : -CRUSTED AREA(S); LEFT DORSAL LUMBAR REGION: SINGLE DRY BROWN CRUSTED AREA, 5 MM IN DIAMETER; POSSIBLY CONSISTENT WITH WART-LIKE LESION NOTED IN CLINICAL OBSERVATION (CAP 26) PROSTATE (PR) : -LARGE; 25 X 20 X 12 MM -GELATINOUS; DIFFUSE: TAN SEMINAL VESICLES (SV) : -GELATINOUS; BILATERAL: TAN MAMMARY, MALE (MM) : -CYST(S); BILATERAL, AXILLARY REGION: MULTIPLE TAN CREAMY SEMIFLUID FILLED CYSTS, UP TO 3 MM IN DIAMETER (CAP 22) -MASS(ES); RIGHT AXILLARY REGION: SINGLE MASS, 35 X 25 X 13 MM, TAN AND FIRM; SAME ON CUT SURFACE; SOME SECTIONS EXUDE A TAN CASEOUS SEMISOLID (CAP 24); REGIONAL LN (AXILLARY) NORMAL (CAP 23) -MASS #2; LEFT INGUINAL REGION: SINGLE MASS, 10 X 10 X 6 MM, TAN AND FIRM; SAME ON CUT SURFACE (CAP 25); REGIONAL LN (INGUINAL) NOT IDENTIFIED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SEVERE SEMINAL VESICLES (SV) : -EDEMA,-PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT MAMMARY, MALE (MM) : -GALACTOCELE,-PRESENT -M-CARCINOMA,-NEOPLASM PRESENT >NOTE:>MASS #1 IS THE CARCINOMA. SEE SKIN FOR DIAGNOSIS OF MASS #2. 2843 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1088 ANIMAL NUMBER: C92858 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/21/00 STUDY DAY OF DEATH: 673 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 526.8 GRAMS DATE AND TIME OF NECROPSY: 02/21/00 14:44 PROSECTOR: DAVID SCHUETTE RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN), LN, AXILLARY (AX) 2844 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1089 ANIMAL NUMBER: C92859 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/01/99 STUDY DAY OF DEATH: 500 STUDY WEEK OF DEATH: 72 TERMINAL BODY WEIGHT: 423.6 GRAMS DATE AND TIME OF NECROPSY: 09/01/99 14:49 PROSECTOR: JERALD W. JOHNSON RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; ROUGH HAIR COAT; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; HEAD TILT; RIGHT; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE DARK RED AREA, UP TO 3 MM IN DIAMETER; SOME AREAS APPEAR TO BE SUNKEN WITH A STELLATE PATTERN; COLLECTED ON ROUTINE SECTIONS KIDNEY (KD) : -DEPRESSED FOCUS(I)/AREA(S); BILATERAL, CORTICAL SURFACE: MULTIPLE AREAS, UP TO 2 MM IN DIAMETER SPLEEN (SP) : -SMALL; 30 X 7 X 4 MM TESTIS (TE) : >NOTE:>TRIMMING COMMENT: ONE TESTIS MISSING FROM WET TISSUE HISTOPATHOLOGY BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERPLASIA BILE DUCT,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : -PIGMENT,-MINIMAL TESTIS (TE) : -UNILATERALLY EXAMINED,-PRESENT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATELY SEVERE 2845 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1090 ANIMAL NUMBER: C92859 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/01/99 STUDY DAY OF DEATH: 500 STUDY WEEK OF DEATH: 72 TERMINAL BODY WEIGHT: 423.6 GRAMS DATE AND TIME OF NECROPSY: 09/01/99 14:49 PROSECTOR: JERALD W. JOHNSON RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 12 X 8 X 6 MM, DARK RED, LOBULATED AND SOFT EPIDIDYMIDES (EP) : >NOTE:>TRIMMING COMMENT: ONE EPIDIDYMIS MISSING FROM WET TISSUE GENERAL COMMENT (GC) -BLOOD FILM TAKEN LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, NONGL (SU) : -ACANTHOSIS / HYPERKERATOSIS,-MINIMAL PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT EPIDIDYMIDES (EP) : -UNILATERALLY EXAMINED,-PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 2846 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1091 ANIMAL NUMBER: C92859 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/01/99 STUDY DAY OF DEATH: 500 STUDY WEEK OF DEATH: 72 TERMINAL BODY WEIGHT: 423.6 GRAMS DATE AND TIME OF NECROPSY: 09/01/99 14:49 PROSECTOR: JERALD W. JOHNSON RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUm /s KULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), HEMATO NEOPLASIA (HN) (SN), 2847 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1092 ANIMAL NUMBER: C92860 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/26/00 STUDY DAY OF DEATH: 678 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 580.0 GRAMS DATE AND TIME OF NECROPSY: 02/26/00 7:35 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >WART LIKE LESION(S); VENTRAL-THORACICLEFT; WEEK 96; VERIFIED AT NECROPSY: NOT OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; LIMITED USE; LIMBS-HIND; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; ROUGH HAIR COAT; VERIFIED AT NECROPSY: NOT APPLICABLE >EYES; RED DISCHARGE; EYES; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; ATAXIC; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY LIVER (LI) : -DIFFUSELY LIGHT; ALL LOBES: TAN SPLEEN (SP) : -MASS(ES); DIFFUSE: MULTIPLE MASSES, UP TO 10 MM IN DIAMETER, RED, TAN, AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (SEE PANCREATIC) -MASS #2; INVOLVING ENTIRE CRANIAL END: SINGLE MASS, 10 MM IN DIAMETER, RED, TAN, HARD, BONE-LIKE; SAME ON CUT SURFACE, ALTHOUGH MASS WAS VERY DIFFICULT TO SECTION (CAP 25); REGIONAL LN (SEE PANCREATIC) HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MODERATE -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL -VACUOLATION, HEPATOCELLULAR,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE -M-OSTEOSARCOMA,-NEOPLASM PRESENT PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL 2848 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1093 ANIMAL NUMBER: C92860 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/26/00 STUDY DAY OF DEATH: 678 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 580.0 GRAMS DATE AND TIME OF NECROPSY: 02/26/00 7:35 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PANCREAS (PA) : -MASS(ES); ALONG SPLEEN: SINGLE MASS, 8 MM IN DIAMETER, RED, GRAY, AND SOFT; SAME ON CUT SURFACE (CAP 23); REGIONAL LN (SEE PANCREATIC) ADRENAL, CORTEX (AC) : -MASS(ES); INVOLVING APPROXIMATELY ONEHALF OF LEFT: SINGLE MASS, 5 MM IN DIAMETER, TAN, HARD, AND BONE-LIKE; MASS VERY DIFFICULT TO SECTION, PORTION OF REMAINING ADRENAL EXUDED OUT OF CORTEX AREA IN AN ATTEMPT TO SECTION MASS (CAP 26); REGIONAL LN (SEE RENAL) TESTIS (TE) : -SMALL; BILATERAL: EACH IS 20 X 13 X 8 MM; BOTH ARE SOFT PANCREAS (PA) : -M-CARCINOMA, ISLET CELL,-NEOPLASM PRESENT -INFILTRATION, FAT, INTERSTITIAL,MODERATE ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-MINIMAL -N-SARCOMA,-NEOPLASM PRESENT TESTIS (TE) : -ATROPHY/DEGENERATION,-MODERATE SPINAL CORD (SC) : -NECROSIS / HEMORRHAGE, WHITE MATTER,MODERATE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY), MODERATE LUNG (LU) : -N-SARCOMA,-NEOPLASM PRESENT 2849 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1094 ANIMAL NUMBER: C92860 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/26/00 STUDY DAY OF DEATH: 678 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 580.0 GRAMS DATE AND TIME OF NECROPSY: 02/26/00 7:35 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY LUNG (LU) : -MASS(ES); ALL LOBES, INVOLVING MOST OF LUNG TISSUE: MULTIPLE MASSES, UP TO 15 X 10 X 8 MM, RED, DARK RED, TAN, AND FIRM; SAME ON CUT SURFACES; COLLECTED INTACT; REGIONAL LN (SEE TRACHEOBRONCHIAL) -MOTTLED; ALL LOBES: RED, DARK RED, AND TAN -UNCOLLAPSED; ALL LOBES STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA: MULTIPLE RED AND TAN ERODED AREAS, UP TO 5 MM IN DIAMETER -THICKENED WALL; DIFFUSE HEART (HT) : -CARDIOMYOPATHY,-SLIGHT THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PITUITARY (PI) : -HYPERPLASIA,-SLIGHT STOMACH, NONGL (SU) : -ACANTHOSIS / HYPERKERATOSIS,-MODERATE -ULCERATION,-MINIMAL -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT SEMINAL VESICLES (SV) : -ATROPHY,-MODERATELY SEVERE CAUSE OF DEATH (CD) : -NEOPLASM, SPLEEN,-PRESENT LN, RENAL (RL) : -N-SARCOMA,-NEOPLASM PRESENT 2850 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1095 ANIMAL NUMBER: C92860 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/26/00 STUDY DAY OF DEATH: 678 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 580.0 GRAMS DATE AND TIME OF NECROPSY: 02/26/00 7:35 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY LN, RENAL (RL) : -LARGE; LEFT: 10 MM IN DIAMETER IS 5 X 3 X 2 MM) (CAP 27) LN, ANT MES/PANC (AP) : -DIFFUSELY RED; (CAP 24) (RIGHT LN, TRACHEOBRON (TB) : -LARGE; UP TO 8 X 5 X 3 MM (CAP 22) -DIFFUSELY RED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DARK RED AND CRUSTED -STAINS-PERINEUM/PERIANAL; YELLOW AND MOIST LN, ANT MES/PANC (AP) : -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL LN, TRACHEOBRON (TB) : -DILATATION, SINUSOIDAL,-PRESENT -HEMORRHAGE,-SLIGHT -N-SARCOMA,-NEOPLASM PRESENT 2851 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1096 ANIMAL NUMBER: C92860 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/26/00 STUDY DAY OF DEATH: 678 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 580.0 GRAMS DATE AND TIME OF NECROPSY: 02/26/00 7:35 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, MANDIBULAR (MN), MAMMARY, MALE (MM), COAGULATING GL (CG), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), PREPUTIAL GLAND (PG), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), VAS DEFERENS (VS), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) (ES), 2852 COVAH^ THE DEVELOPMENT SERVICES COMPANY Sponsor: 3M St. Paul, Minnesota FINAL REPORT Study Title: 104-Week Dietary Chronic Toxicity and Carcinogenicity Study with Perfluorooctane Sulfonic Acid Potassium Salt (PFOS; T-6295) in Rats Author: Peter J. Thomford, PhD Study Completion Date: January 2, 2002 Testing Facility: Covance Laboratories Inc. 3301 Kinsman Boulevard Madison, Wisconsin 53704-2595 Laboratory Study Identification: Covance 6329-183 Sponsor Study Identification: 3M T-6295 Volume VIII of IX Page 2853 of 4068 Covance 6329-183 3M T-6295 VOLUME VIII OF IX CONTENTS Page APPENDIX 6 (Continued)........................................................................................... 2855 Individual Anatomic Pathology Data....................................................................... 2856 2854 APPENDIX 6 (Continued) Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 2855 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1097 ANIMAL NUMBER: C92861 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 226.3 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 8:46 PROSECTOR: JODI THIEREN RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN STATUS LF ADRENAL (AD0) RT ADRENAL (AD1) BRAIN (BR) LF KIDNEY (KD0) RT KIDNEY (KD1) LIVER (LI) LUNG (LU) LF OVARY (OV0) RT OVARY (OV1) SPLEEN (SP) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) UTERUS (UT) ------------------------------------ 7.8642 -------------------------------------------------- ------------------------------------ 3.4757 % -------------------------------------------------- -------------------------------------------------------------------------------------------- NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN WEIGHT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS 2856 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1098 ANIMAL NUMBER: C92861 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 226.3 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 8:46 PROSECTOR: JODI THIEREN RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) 2857 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1099 ANIMAL NUMBER: C92862 SEX: FEMALE DATE OF DEATH: 04/20/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/20/99 10:23 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 281.2 GRAMS PROSECTOR: GREGORY CODDINGTON RECORDER: TONY HANFELD PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) LF ADRENAL (AD0) RT ADRENAL (AD1) BRAIN (BR) LF KIDNEY (KD0) RT KIDNEY (KD1) LIVER (LI) LUNG (LU) LF OVARY (OV0) RT OVARY (OV1) SPLEEN (SP) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) UTERUS (UT) .0474 .0341 2.0523 1.1945 1.2160 9.3278 1.2262 .0431 .0686 .3610 .0145 .0135 .9292 .0169 % .0121 % .7298 % .4248 % .4324 % 3.3171 % .4361 % .0153 % .0244 % .1284 % .0052 % .0048 % .3304 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0231 .0166 1.0000 .5820 .5925 4.5450 .5975 .0210 .0334 .1759 .0071 .0066 .4528 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN -MALOCCLUSION; RIGHT UPPER INCISOR KIDNEY (KD) : -MINERALIZATION, TUBULAR,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : -INFILTRATE, MACROPHAGE, PIGMENTED MINIMAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2858 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1100 ANIMAL NUMBER: C92862 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 281.2 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 10:23 PROSECTOR: GREGORY CODDINGTON RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LIVER (LI), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 2859 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1101 ANIMAL NUMBER: C92863 SEX: FEMALE DATE OF DEATH: 11/15/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 11/15/99 13:44 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 575 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: 483.8 GRAMS PROSECTOR: TODD ACKER RECORDER: TODD ACKER PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MISSING; TAIL-DISTAL; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; BLACK SKIN; TAIL; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -RED FOCUS(I)/AREA(S); DIAPHRAGMATIC SURFACE OF LEFT MEDIAN LOBE: SINGLE DARK RED FOCUS, 3 MM IN DIAMETER (CAP 21) SPLEEN (SP) : -IRREGULAR SHAPE -LIGHT FOCUS(I)/AREA(S); DIFFUSE: MULTIPLE TAN AREAS, UP TO 6 X 3 MM -ADHESION(S); PARIETAL SURFACE TO LEFT OVARY: MULTIPLE TAN AND FIBROUS ADHESIONS; COLLECTED INTACT HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -NECROSIS, INDIVIDUAL HEPATOCYTE,MINIMAL -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL -ANGIECTASIS,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, TUBULAR,-MINIMAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATELY SEVERE -FIBROSIS, CAPSULAR,-PRESENT PANCREAS (PA) : -ATROPHY, ACINAR,-SLIGHT MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,-MODERATE MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,-MODERATE 2860 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1102 ANIMAL NUMBER: C92863 SEX: FEMALE DATE OF DEATH: 11/15/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 11/15/99 13:44 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 575 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: 483.8 GRAMS PROSECTOR: TODD ACKER RECORDER: TODD ACKER PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); ATTACHED TO DORSAL SIDE OF RIGHT ASPECT: SINGLE MASS, 5 X 3 X 2 MM, RED, DARK RED AND SOFT MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT PECTORAL REGION: SINGLE MASS, 30 X 20 X 10 MM, TAN AND SOFT; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (AXILLARY) NORMAL (CAP 23) -THICKENED; ALL REGIONS, DIFFUSE: COLLECTED ON ROUTINE SECTION THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATE LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-SLIGHT -INFLAMMATION, VASCULAR,-SLIGHT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT RECTUM (RE) : -INFLAMMATION, VASCULAR,-SLIGHT MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT -B-FIBROADENOMA,-NEOPLASM PRESENT -HYPERPLASIA, GLANDULAR,-MODERATE URINARY BLADDER (UB) : -INFLAMMATION, VASCULAR,-MINIMAL VAGINA (VA) : -HYPERPLASIA, MUCINOUS,-SEVERE CAUSE OF DEATH (CD) : -VASCULITIS, SYSTEMIC,-PRESENT 2861 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1103 ANIMAL NUMBER: C92863 SEX: FEMALE DATE OF DEATH: 11/15/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 11/15/99 13:44 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 575 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: 483.8 GRAMS PROSECTOR: TODD ACKER RECORDER: TODD ACKER PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY TAIL (TI) : -DIFFUSELY RED; 60 MM FROM REMAINING TIP: DARK RED; COLLECTED LN, ANT MES/PANC (AP) : -DIFFUSELY RED; PANCREATIC (CAP 22) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -TAIL - BENT/MISSING; DISTAL ONE THIRD: MISSING; REMAINING PORTION COLLECTED TAIL (TI) : -INFLAMMATION, VASCULAR,-SLIGHT LN, ANT MES/PANC (AP) : -HEMORRHAGE,-PRESENT -HYPERPLASIA, LYMPHOPLASMACYTIC,-SLIGHT 2862 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1104 ANIMAL NUMBER: C92863 SEX: FEMALE DATE OF DEATH: 11/15/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 11/15/99 13:44 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 575 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: 483.8 GRAMS PROSECTOR: TODD ACKER RECORDER: TODD ACKER PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), SKIN (SK), UTERUS (UT), CERVIX (CV), HEMATO NEOPLASIA (HN), LN, AXILLARY (AX) 2863 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1105 ANIMAL NUMBER: C92864 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 218.1 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 8:53 PROSECTOR: LYNN HORNER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN STATUS LF ADRENAL (AD0) RT ADRENAL (AD1) BRAIN (BR) LF KIDNEY (KD0) RT KIDNEY (KD1) LIVER (LI) LUNG (LU) LF OVARY (OV0) RT OVARY (OV1) SPLEEN (SP) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) UTERUS (UT) ------------------------------------ 8.3146 -------------------------------------------------- ------------------------------------ 3.8124 % -------------------------------------------------- -------------------------------------------------------------------------------------------- NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN WEIGHT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS 2864 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1106 ANIMAL NUMBER: C92864 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 218.1 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 8:53 PROSECTOR: LYNN HORNER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) 2865 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1107 ANIMAL NUMBER: C92865 SEX: FEMALE DATE OF DEATH: 07/12/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 07/12/99 9:49 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DEATH: 449 STUDY WEEK OF DEATH: 65 TERMINAL BODY WEIGHT: NOT ENTERED PROSECTOR: DAVID SCHUETTE RECORDER: MICHELE MARGGI PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LUNG (LU) : -DIFFUSELY RED; ALL LOBES: DARK RED PITUITARY (PI) : -MASS(ES); SINGLE MASS, 8 X 6 X 5 MM, DARK RED, TAN, AND SOFT HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -HEMORRHAGE,-MINIMAL -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL LIVER (LI) : -CONGESTION,-PRESENT SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE PANCREAS (PA) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL ADRENAL, CORTEX (AC) : -CONGESTION,-PRESENT -PIGMENT,-MINIMAL SPINAL CORD (SC) : -HEMORRHAGE,-MINIMAL THYMUS (TH) : -HEMORRHAGE,-PRESENT LUNG (LU) : -CONGESTION,-PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT 2866 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1108 ANIMAL NUMBER: C92865 SEX: FEMALE DATE OF DEATH: 07/12/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 07/12/99 9:49 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DEATH: 449 STUDY WEEK OF DEATH: 65 TERMINAL BODY WEIGHT: NOT ENTERED PROSECTOR: DAVID SCHUETTE RECORDER: MICHELE MARGGI PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -THICKENED; ALL REGIONS, DIFFUSE: TAN; COLLECTED ON ROUTINE SECTION CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 2867 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1109 ANIMAL NUMBER: C92865 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 07/12/99 STUDY DAY OF DEATH: 449 STUDY WEEK OF DEATH: 65 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 07/12/99 9:49 PROSECTOR: DAVID SCHUETTE RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG), GENERAL COMMENT (GC) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), KIDNEY (KD), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) (CE), 2868 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1110 ANIMAL NUMBER: C92866 SEX: FEMALE DATE OF DEATH: 04/21/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/21/00 9:52 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 341.4 GRAMS PROSECTOR: JAMES T. CALLEN RECORDER: LAURIE J. SCHULLER PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) KIDNEY (KD) : -CYST(S); RIGHT ON CORTICAL SURFACE NEAR CAUDAL POLE: SINGLE CLEAR FLUID FILLED CYST, 1 MM IN DIAMETER OVARY (OV) : -CYST(S); RIGHT: SINGLE CLEAR FLUID FILLED CYST, 8 MM IN DIAMETER HISTOPATHOLOGY EYE (EY) : -DEGENERATION, RETINAL,-MODERATE BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, PELVIC,-MODERATE -HYPERPLASIA, TRANSITIONAL CELL,-SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATE -HYPERTROPHY, CORTICAL CELL,-SLIGHT -DEGENERATION, CYSTIC / HEMORRHAGIC,SLIGHT -PIGMENT,-SLIGHT OVARY (OV) : -CYST,-PRESENT 2869 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1111 ANIMAL NUMBER: C92866 SEX: FEMALE DATE OF DEATH: 04/21/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/21/00 9:52 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 341.4 GRAMS PROSECTOR: JAMES T. CALLEN RECORDER: LAURIE J. SCHULLER PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 10 X 10 X 5 MM, DARK RED AND SOFT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN MARROW, FEMUR (FM) : -HYPOCELLULAR, MARROW,-MODERATE LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT -HYPERPLASIA,-MINIMAL MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT -HYPERPLASIA, GLANDULAR,-SLIGHT UTERUS (UT) : -DILATATION,-MODERATE CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2870 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1112 ANIMAL NUMBER: C92866 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 341.4 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 9:52 PROSECTOR: JAMES T. CALLEN RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 2871 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1113 ANIMAL NUMBER: C92867 SEX: FEMALE DATE OF DEATH: 04/20/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/20/00 8:37 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 588.2 GRAMS PROSECTOR: TODD ACKER RECORDER: TONE EVERTS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); VENTRAL-ABDOMINAL; WEEK 104; 15 X 15MM; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-SLIGHT -FIBROSIS, PERIBILIARY,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, PELVIC,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SLIGHT PANCREAS (PA) : -INFILTRATION, FAT, INTERSTITIAL,SLIGHT ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATE -HYPERTROPHY, CORTICAL CELL,-MODERATE -PIGMENT,-SLIGHT ADRENAL, MEDULLA (MA) : -UNILATERALLY EXAMINED,-PRESENT MARROW, FEMUR (FM) : -HYPOCELLULAR, MARROW,-MODERATE 2872 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1114 ANIMAL NUMBER: C92867 SEX: FEMALE DATE OF DEATH: 04/20/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/20/00 8:37 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 588.2 GRAMS PROSECTOR: TODD ACKER RECORDER: TONE EVERTS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -LARGE; 5 X 4 X 4 MM, APPEARS SWOLLEN MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT PERINEAL REGION: SINGLE MASS, 20 X 15 X 10 MM, DARK RED, TAN, AND FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL: COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN DARK RED; HEART (HT) : -CARDIOMYOPATHY,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -B-FIBROADENOMA,-NEOPLASM PRESENT -HYPERPLASIA, GLANDULAR,-MODERATE VAGINA (VA) : -HYPERPLASIA, MUCINOUS,-MODERATE CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MANDIBULAR (MN) : -HEMORRHAGE,-PRESENT 2873 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1115 ANIMAL NUMBER: C92867 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 588.2 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 8:37 PROSECTOR: TODD ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG),STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP),JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM(DP), CAVITY, THORACIC (TA),LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), HEMATO NEOPLASIA (HN) 2874 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1116 ANIMAL NUMBER: C92868 SEX: FEMALE DATE OF DEATH: 04/19/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/19/00 11:24 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 551.6 GRAMS PROSECTOR: TONY HANFELD RECORDER: LAURIE J. SCHULLER PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL LIVER-OIL RED O (LI0) : -VACUOLES, OIL RED O-POSITIVE, HEPATOCELLULAR,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, PELVIC,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SLIGHT PANCREAS (PA) : -INFILTRATION, FAT, INTERSTITIAL,MINIMAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATE -HYPERTROPHY, CORTICAL CELL,-MINIMAL -HYPERPLASIA, CORTICAL,-MINIMAL 2875 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1117 ANIMAL NUMBER: C92868 SEX: FEMALE DATE OF DEATH: 04/19/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/19/00 11:24 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 551.6 GRAMS PROSECTOR: TONY HANFELD RECORDER: LAURIE J. SCHULLER PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY LN, MESENTERIC (MS) : -DIFFUSELY DARK; DARK GREEN OVARY (OV) : -CYST(S); RIGHT: SINGLE CLEAR FLUID FILLED CYST, 8 MM IN DIAMETER PITUITARY (PI) : -RED FOCUS(I)/AREA(S); RIGHT ANTERIOR ASPECT: SINGLE DARK RED AREA, 1 MM IN DIAMETER MAMMARY, FEMALE (MF) : -CYST(S); ABDOMINAL REGION: MULTIPLE WHITE FLUID FILLED CYSTS, UP TO 10 X 6 X 2 MM (CAP 22) UTERUS (UT) : -CYST(S); BILATERAL WITHIN WALLS: MULTIPLE CLEAR AND DARK RED FLUID FILLED CYSTS, UP TO 3 MM IN DIAMETER GENERAL COMMENT (GC) : -BLOOD FILM TAKEN LN, MESENTERIC (MS) >UNREMARKABLE OVARY (OV) : -CYST,-PRESENT : THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATE HEART (HT) : -CARDIOMYOPATHY,-MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-SLIGHT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT -HYPERPLASIA,-MINIMAL MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE -HYPERPLASIA, GLANDULAR,-SLIGHT UTERUS (UT) : -HYPERPLASIA, CYSTIC, ENDOMETRIAL,SLIGHT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2876 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1118 ANIMAL NUMBER: C92868 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 551.6 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 11:24 PROSECTOR: TONY HANFELD RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUm /s KULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), ADRENAL, MEDULLA (MA), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 2877 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1119 ANIMAL NUMBER: C92869 SEX: FEMALE DATE OF DEATH: 04/20/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/20/00 8:50 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 435.4 GRAMS PROSECTOR: TROY ELLENBECKER RECORDER: JERALD W. JOHNSON PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY KIDNEY (KD) : -LARGE PELVIS(ES); RIGHT HISTOPATHOLOGY LIVER (LI) : -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -HYPERPLASIA, BILE DUCT,-SLIGHT -FIBROSIS, PERIBILIARY,-SLIGHT -HYPERTROPHY, HEPATOCELLULAR, PERIPORTAL,-MODERATE KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, PELVIC,-SLIGHT -CYST,-PRESENT SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SLIGHT ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATE -PIGMENT,-MINIMAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),SLIGHT 2878 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1120 ANIMAL NUMBER: C92869 SEX: FEMALE DATE OF DEATH: 04/20/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/20/00 8:50 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 435.4 GRAMS PROSECTOR: TROY ELLENBECKER RECORDER: JERALD W. JOHNSON PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN HEART (HT) : -CARDIOMYOPATHY,-MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-SLIGHT PITUITARY (PI) : -HYPERPLASIA,-SLIGHT MAMMARY, FEMALE (MF) : -HYPERPLASIA, GLANDULAR,-SLIGHT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2879 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1121 ANIMAL NUMBER: C92869 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 435.4 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 8:50 PROSECTOR: TROY ELLENBECKER RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 2880 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1122 ANIMAL NUMBER: C92870 SEX: FEMALE DATE OF DEATH: 04/17/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/17/00 8:10 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 729 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 332.8 GRAMS PROSECTOR: JODI THIEREN RECORDER: JODI THIEREN PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); AXILLARY REGION-LEFT; WEEK 99; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; ATAXIC; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; HEAD TILT; LEFT; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; RECUMBENT; LATERAL; VERIFIED AT NECROPSY: NOT APPLICABLE >MASS(ES); INGUINAL REGION-LEFT; WEEK 105; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) HISTOPATHOLOGY BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-SLIGHT -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL -CYST, BILIARY,-PRESENT KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL -MINERALIZATION, TUBULAR,-MINIMAL -HYPERPLASIA, TRANSITIONAL CELL,MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATELY SEVERE ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-SLIGHT -HYPERTROPHY, CORTICAL CELL,-MODERATE -DEGENERATION, CYSTIC / HEMORRHAGIC,MINIMAL -PIGMENT,-SLIGHT 2881 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1123 ANIMAL NUMBER: C92870 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/17/00 STUDY DAY OF DEATH: 729 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 332.8 GRAMS DATE AND TIME OF NECROPSY: 04/17/00 8:10 PROSECTOR: JODI THIEREN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY OVARY (OV) : -CYST(S); RIGHT: SINGLE CLEAR FLUID FILLED CYST, 4 MM IN DIAMETER PITUITARY (PI) : -MASS(ES); SINGLE MASS, 12 X 8 X 8 MM, RED, DARK RED, GRAY AND FIRM MAMMARY, FEMALE (MF) : -MASS(ES); LEFT AXILLARY REGION: SINGLE MASS, 30 X 30 X 10 MM, TAN AND FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (AXILLARY) NORMAL (CAP 21) -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION -MASS #2; PERINEAL REGION: SINGLE MASS, 30 X 20 X 15 MM, TAN AND FIRM, SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED; POSSIBLY CONSISTENT WITH MASS (INGUINAL-LEFT) AS NOTED IN CLINICAL OBSERVATIONS OVARY (OV) : -CYST,-PRESENT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MINIMAL -CYST,-PRESENT THYROID (TY) : -HYPERPLASIA, C-CELL,-SLIGHT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -B-FIBROADENOMA,- 2 NEOPLASMS PRESENT -HYPERPLASIA, GLANDULAR,-MODERATE -B-ADENOMA,-NEOPLASM PRESENT >NOTE:>MASSES 1 AND 2 ARE PRIMARILY FIBROADENOMAS A SMALL ADENOMA WAS ALSO NOTED IN MASS 2 2882 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1124 ANIMAL NUMBER: C92870 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/17/00 STUDY DAY OF DEATH: 729 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 332.8 GRAMS DATE AND TIME OF NECROPSY: 04/17/00 8:10 PROSECTOR: JODI THIEREN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY UTERUS (UT) : -CYST(S); RIGHT HORN, WITHIN WALL: SINGLE CLEAR FLUID FILLED CYST, 18 X 5 X 5 MM VAGINA (VA) : -SEMIFLUID MATERIAL; WITHIN LUMEN: CONTAINS A TAN MUCOID SEMIFLUID MATERIAL GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL: LOWER INCISORS -DISCHARGE-OCULAR; LEFT: DARK RED AND DRY MATERIAL -ANIMAL THIN UTERUS (UT) : -B-POLYP, ENDOMETRIAL, STROMAL,NEOPLASM PRESENT -HYPERPLASIA, CYSTIC, ENDOMETRIAL,SEVERE CERVIX (CV) : -HYPERPLASIA, MUCINOUS,-MODERATE VAGINA (VA) : >UNREMARKABLE CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT LN, AXILLARY (AX) : -HYPERPLASIA, LYMPHOPLASMACYTIC,-SLIGHT 2883 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1125 ANIMAL NUMBER: C92870 SEX: FEMALE DATE OF DEATH: 04/17/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/17/00 8:10 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 729 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 332.8 GRAMS PROSECTOR: JODI THIEREN RECORDER: JODI THIEREN PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), HEMATO NEOPLASIA (HN) (SG), 2884 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1126 ANIMAL NUMBER: C92871 SEX: FEMALE DATE OF DEATH: 04/19/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/19/00 11:46 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 641.1 GRAMS PROSECTOR: JARED FISHEL RECORDER: LAURIE J. SCHULLER PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); VENTRAL-THORACIC-LEFT; WEEK 76; 50 X 50MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); INGUINAL REGION-LEFT; WEEK 94; 50 X 50MM; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -LIGHT FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE TAN AREAS, UP TO 3 MM IN DIAMETER; COLLECTED ON ROUTINE SECTIONS ADRENAL, CORTEX (AC) : -LARGE; RIGHT: 9 MM IN DIAMETER -SMALL; LEFT: 3 X 2 X 2 MM HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MODERATE -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, PERIPORTAL,-SLIGHT LIVER-OIL RED O (LI0) : -VACUOLES, OIL RED O-POSITIVE, HEPATOCELLULAR,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SLIGHT -MINERALIZATION, PELVIC,-SLIGHT SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC / HEMORRHAGIC,SEVERE -PIGMENT,-SLIGHT 2885 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1127 ANIMAL NUMBER: C92871 SEX: FEMALE DATE OF DEATH: 04/19/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/19/00 11:46 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 641.1 GRAMS PROSECTOR: JARED FISHEL RECORDER: LAURIE J. SCHULLER PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 8 X 7 X 5 MM, DARK RED, TAN, AND SOFT MAMMARY, FEMALE (MF) : -MASS(ES); LEFT AXILLARY REGION: SINGLE MASS, 70 X 60 X 30 MM, TAN, FIRM, AND LOBULATED; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (AXILLARY) NORMAL (CAP 22) -MASS #2; LEFT PERINEAL REGION: SINGLE MASS, DARK RED, TAN, VASCULAR, FIRM, AND LOBULATED, 60 X 50 X 30 MM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (INGUINAL) NORMAL (CAP 23); OVERLYING SKIN HAS A SINGLE DARK RED AND MOIST ULCERATED AREA, 10 MM IN DIAMETER; COLLECTED WITH MASS ADRENAL, MEDULLA (MA) : -HYPERPLASIA, MEDULLARY,-SLIGHT -B-PHEOCHROMOCYTOMA,-NEOPLASM PRESENT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY) MINIMAL HEART (HT) : -CARDIOMYOPATHY,-SLIGHT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -M-CARCINOMA,-NEOPLASM PRESENT -CYST / GALACTOCELE,-PRESENT -B-FIBROADENOMA,- 2 NEOPLASMS PRESENT -HYPERPLASIA, GLANDULAR,-SLIGHT >NOTE:>MASS #2 IS THE CARCINOMA 2886 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1128 ANIMAL NUMBER: C92871 SEX: FEMALE DATE OF DEATH: 04/19/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/19/00 11:46 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 641.1 GRAMS PROSECTOR: JARED FISHEL RECORDER: LAURIE J. SCHULLER PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS #3; LEFT INGUINAL REGION: SINGLE MASS, 15 X 10 X 5 MM, TAN AND FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (INGUINAL) NORMAL (CAP 23) UTERUS (UT) : -CYST(S); BILATERAL WITHIN WALLS: MULTIPLE CLEAR AND RED FLUID FILLED CYSTS, UP TO 4 MM IN DIAMETER GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN GENERAL INFORMATION (XX) : >NOTE:>TRIMMING COMMENT: ANIMAL ID MISSING AT TRIMMING UTERUS (UT) : -HYPERPLASIA, CYSTIC, ENDOMETRIAL,MODERATE VAGINA (VA) : -HYPERPLASIA, MUCINOUS,-MODERATE CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, AXILLARY (AX) : -HEMORRHAGE,-PRESENT LN, INGUINAL (IN) : -N-CARCINOMA,-NEOPLASM PRESENT 2887 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1129 ANIMAL NUMBER: C92871 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 641.1 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 11:46 PROSECTOR: JARED FISHEL RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), HEMATO NEOPLASIA (HN) (SE), 2888 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1130 ANIMAL NUMBER: C92872 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/28/99 STUDY DAY OF DEATH: 618 STUDY WEEK OF DEATH: 89 TERMINAL BODY WEIGHT: 485.2 GRAMS DATE AND TIME OF NECROPSY: 12/28/99 14:48 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); VENTRAL-ABDOMINAL; WEEK 41; 80 X 70MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); INGUINAL REGION-LEFT; WEEK 83; 30 X 20MM; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; VENTRALABDOMINAL-LEFT; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) ADRENAL, CORTEX (AC) : -LARGE; LEFT: 8 X 6 X 6 MM (RIGHT IS 5 MM IN DIAMETER) OVARY (OV) : -CYST(S); LEFT: SINGLE CLEAR FLUID FILLED CYST, 3 MM IN DIAMETER HISTOPATHOLOGY BRAIN (BR) : >UNREMARKABLE LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-MODERATE SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC / HEMORRHAGIC,SEVERE OVARY (OV) : -CYST,-PRESENT MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW, -SLIGHT MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW, -MODERATE LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL 2889 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1131 ANIMAL NUMBER: C92872 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/28/99 STUDY DAY OF DEATH: 618 STUDY WEEK OF DEATH: 89 TERMINAL BODY WEIGHT: 485.2 GRAMS DATE AND TIME OF NECROPSY: 12/28/99 14:48 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 7 X 7 X 5 MM, DARK RED, TAN AND SOFT MAMMARY, FEMALE (MF) : -MASS(ES); LEFT INGUINAL REGION: SINGLE MASS, 80 X 75 X 45 MM (138.2 GRAMS), RED, DARK RED, GREY, TAN, LOBULATED, VASCULAR AND FIRM; SAME ON CUT SURFACE; OVERLYING SKIN HAS A SINGLE DARK RED, TAN AND DRY CRUSTED AREA, 30 X 25 MM; COLLECTED INTACT WITH MASS; REGIONAL LN (INGUINAL) NORMAL (CAP 21) -MASS #2; LEFT INGUINAL REGION, CAUDAL TO MASS 1: SINGLE MASS, 25 X 25 X 20 MM, DARK RED, TAN AND FIRM; SAME ON CUT SURFACE (COLLECTED); REGIONAL LN (INGUINAL) NORMAL (CAP 21) UTERUS (UT) : -CYST(S); PROXIMAL END OF LEFT HORN, WITHIN LUMEN: SINGLE CLEAR FLUID FILLED CYST, 9 X 5 X 5 MM LUNG (LU) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT RECTUM (RE) : -PARASITISM,-PRESENT MAMMARY, FEMALE (MF) : -M-CARCINOMA,-NEOPLASM PRESENT -B-FIBROADENOMA,-NEOPLASM PRESENT >NOTE:>MASS #1 IS THE FIBROADENOMA UTERUS (UT) : -DILATATION,-MODERATELY SEVERE 2890 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1132 ANIMAL NUMBER: C92872 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/28/99 STUDY DAY OF DEATH: 618 STUDY WEEK OF DEATH: 89 TERMINAL BODY WEIGHT: 485.2 GRAMS DATE AND TIME OF NECROPSY: 12/28/99 14:48 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN CAUSE OF DEATH (CD) : -NEOPLASM, MAMMARY GLAND,-PRESENT LN, INGUINAL (IN) : >TISSUE MISSING 2891 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1133 ANIMAL NUMBER: C92872 SEX: FEMALE DATE OF DEATH: 12/28/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 12/28/99 14:48 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 618 STUDY WEEK OF DEATH: 89 TERMINAL BODY WEIGHT: 485.2 GRAMS PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), BONE, STERNUM SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) (SB), 2892 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1134 ANIMAL NUMBER: C92873 SEX: FEMALE DATE OF DEATH: 04/21/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/21/00 7:29 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 441.8 GRAMS PROSECTOR: TERRI L. WILLIAMS RECORDER: LAURIE J. SCHULLER PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; ALOPECIA; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -RED FOCUS(I)/AREA(S); ALONG MARGIN OF RIGHT LATERAL LOBE, NEAR HILUS: SINGLE DARK RED AREA, 10 X 5 MM (CAP 23) -CYST(S); PAPILLARY PROCESS: SINGLE CLEAR FLUID FILLED CYST, 1 MM IN DIAMETER (CAP 22) HISTOPATHOLOGY BRAIN (BR) : >UNREMARKABLE LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, PERIPORTAL,-MINIMAL -CYST, BILIARY,-PRESENT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -INFARCT,-SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SLIGHT PANCREAS (PA) : -INFILTRATION, FAT, INTERSTITIAL,SLIGHT ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATE -PIGMENT,-MINIMAL 2893 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1135 ANIMAL NUMBER: C92873 SEX: FEMALE DATE OF DEATH: 04/21/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/21/00 7:29 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 441.8 GRAMS PROSECTOR: TERRI L. WILLIAMS RECORDER: LAURIE J. SCHULLER PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 10 X 5 X 5 MM, RED, DARK RED, GRAY, AND SOFT STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: MULTIPLE BLACK AREAS, UP TO 3 X 1 MM SKIN (SK) : -ALOPECIA-DIFFUSE; ENTIRE VENTRAL SURFACE: COLLECTED ON ROUTINE SECTION MAMMARY, FEMALE (MF) : -CYST(S); BILATERAL INGUINAL REGIONS: MULTIPLE TAN CREAMY FLUID FILLED CYSTS, UP TO 10 MM IN DIAMETER (CAP 24) ADRENAL, MEDULLA (MA) : -B-PHEOCHROMOCYTOMA,-NEOPLASM PRESENT MARROW, FEMUR (FM) : -HYPOCELLULAR, MARROW,-SLIGHT SPINAL CORD (SC) : -DEGENERATION, AXONAL,-SLIGHT -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MINIMAL HEART (HT) : -CARDIOMYOPATHY,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : >UNREMARKABLE SKIN (SK) : >UNREMARKABLE MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT -B-FIBROADENOMA,-NEOPLASM PRESENT UTERUS (UT) : -DILATATION,-MODERATE 2894 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1136 ANIMAL NUMBER: C92873 SEX: FEMALE DATE OF DEATH: 04/21/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/21/00 7:29 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 441.8 GRAMS PROSECTOR: TERRI L. WILLIAMS RECORDER: LAURIE J. SCHULLER PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY UTERUS (UT) : -CYST(S); LEFT HORN WITHIN LUMEN: SINGLE CLEAR FLUID FILLED CYST, 6 X 3 X 3 MM GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN VAGINA (VA) : -HYPERPLASIA, MUCINOUS,-SLIGHT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2895 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1137 ANIMAL NUMBER: C92873 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 441.8 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 7:29 PROSECTOR: TERRI L. WILLIAMS RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, STERNUM (SE), BONE, STERNUM (SB), LUNG MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), CERVIX (CV), HEMATO NEOPLASIA (HN) (LU), 2896 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1138 ANIMAL NUMBER: C92874 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 544.5 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 8:26 PROSECTOR: CHANESTA ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); VENTRAL-ABDOMINAL; WEEK 93; 40 X 30MM; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -NECROSIS, INDIVIDUAL HEPATOCYTE,MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL LIVER-OIL RED O (LI0) : -VACUOLES, OIL RED O-POSITIVE, HEPATOCELLULAR,-MODERATE KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, PELVIC,-SEVERE -B-LIPOMA,-NEOPLASM PRESENT SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SLIGHT ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATE -HYPERTROPHY, CORTICAL CELL,-MINIMAL 2897 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1139 ANIMAL NUMBER: C92874 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 544.5 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 8:26 PROSECTOR: CHANESTA ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 8 X 6 X 4 MM, DARK RED, TAN, FIRM AND LOBULATED MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT INGUINAL REGION: SINGLE MASS, 40 X 35 X 20 MM, TAN AND FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN MARROW, FEMUR (FM) : -HYPOCELLULAR, MARROW,-MODERATE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY) MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-MODERATE PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT -B-FIBROADENOMA,-NEOPLASM PRESENT VAGINA (VA) : -HYPERPLASIA, MUCINOUS,-SLIGHT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2898 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1140 ANIMAL NUMBER: C92874 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 544.5 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 8:26 PROSECTOR: CHANESTA ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), HEMATO NEOPLASIA (HN) (SG), 2899 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1141 ANIMAL NUMBER: C92875 SEX: FEMALE DATE OF DEATH: 10/24/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 10/24/99 7:25 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 553 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: 261.7 GRAMS PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; RED HAIR COAT; HEADENTIRE; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; YELLOW HAIR COAT; VERIFIED AT NECROPSY: OBSERVED >DISCHARGE; NASAL; RED IN COLOR; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) ADRENAL, CORTEX (AC) : -LARGE; LEFT: 8 X 5 X 3 MM X 3 X 3 MM) LN, MESENTERIC (MS) : -DIFFUSELY DARK; GREEN (RIGHT IS 4 HISTOPATHOLOGY BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-MINIMAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATE -PIGMENT,-SLIGHT LN, MESENTERIC (MS) : >UNREMARKABLE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATE LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT 2900 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1142 ANIMAL NUMBER: C92875 SEX: FEMALE DATE OF DEATH: 10/24/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 10/24/99 7:25 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 553 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: 261.7 GRAMS PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 13 X 8 X 5 MM, RED, DARK RED, TAN, AND SOFT STOMACH, GL (ST) : -EROSION/ULCERATION; MUCOSA: SINGLE BROWN, ERODED AREA, 3 MM IN DIAMETER MAMMARY, FEMALE (MF) : -CYST(S); ALL REGIONS, DIFFUSE: MULTIPLE TAN, CREAMY SEMIFLUID FILLED CYSTS, UP TO 8 MM IN DIAMETER (CAP 21) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL LOWER INCISORS -DISCHARGE-OCULAR; BILATERAL: DARK RED AND CRUSTED -ANIMAL THIN -STAINS-PERINEUM/PERIANAL; YELLOW AND MOIST -DISCHARGE-NASAL; DARK RED AND CRUSTED -STAINS - SKIN/HAIRCOAT; TOP OF HEAD: RED AND DRY STOMACH, GL (ST) : >UNREMARKABLE MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT URINARY BLADDER (UB) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL VAGINA (VA) : -LUMEN, EXUDATE,-PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 2901 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1143 ANIMAL NUMBER: C92875 SEX: FEMALE DATE OF DEATH: 10/24/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 10/24/99 7:25 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 553 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: 261.7 GRAMS PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), OVARY (OV), BONE, FEMUR(FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), UTERUS (UT), CERVIX (CV), HEMATO NEOPLASIA (HN) (SN), 2902 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1144 ANIMAL NUMBER: C92876 SEX: FEMALE DATE OF DEATH: 04/20/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/20/99 8:52 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 353.5 GRAMS PROSECTOR: NANCY DIEDRICH RECORDER: TONY HANFELD PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) LF ADRENAL (AD0) RT ADRENAL (AD1) BRAIN (BR) LF KIDNEY (KD0) RT KIDNEY (KD1) LIVER (LI) LUNG (LU) LF OVARY (OV0) RT OVARY (OV1) SPLEEN (SP) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) UTERUS (UT) .0292 .0329 2.1997 1.3059 1.3705 8.7983 1.6995 .0538 .0463 .5329 .0101 .0176 .5771 .0083 % .0093 % .6223 % .3694 % .3877 % 2.4889 % .4808 % .0152 % .0131 % .1507 % .0029 % .0050 % .1633 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0133 .0150 1.0000 .5937 .6230 3.9998 .7726 .0245 .0210 .2423 .0046 .0080 .2624 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >MASS(ES); VENTRAL-ABDOMINAL-RIGHT; WEEK 53; 10 X 10MM; VERIFIED AT NECROPSY: OBSERVED LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -NECROSIS, INDIVIDUAL HEPATOCYTE,MINIMAL -HYPERPLASIA, BILE DUCT,-SLIGHT -VACUOLATION, HEPATOCELLULAR,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, TUBULAR,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT 2903 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1145 ANIMAL NUMBER: C92876 SEX: FEMALE DATE OF DEATH: 04/20/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/20/99 8:52 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 353.5 GRAMS PROSECTOR: NANCY DIEDRICH RECORDER: TONY HANFELD PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT INGUINAL REGION: SINGLE MASS, 10 X 10 X 5 MM, TAN, FIRM AND GELATINOUS; SAME ON CUT SURFACE (CAP 20); REGIONAL LN (INGUINAL) NORMAL (CAP 21) GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MINIMAL ADRENAL, CORTEX (AC) : -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL THYMUS (TH) : -HEMORRHAGE,-PRESENT PITUITARY (PI) : -HYPERPLASIA,-MINIMAL HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL MAMMARY, FEMALE (MF) : -M-FIBROSARCOMA,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, INGUINAL (IN) : -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL -HEMORRHAGE,-PRESENT 2904 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1146 ANIMAL NUMBER: C92876 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 353.5 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 8:52 PROSECTOR: NANCY DIEDRICH RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 2905 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1147 ANIMAL NUMBER: C92877 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 493.7 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 10:35 PROSECTOR: TROY ELLENBECKER RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE DARK RED AREAS, PINPOINT AND UP TO 2 MM; COLLECTED ON ROUTINE SECTIONS HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MODERATE -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MODERATE -CONGESTION,-PRESENT -HYPERPLASIA, BILE DUCT,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, PELVIC,-SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SLIGHT ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-SLIGHT -ANGIECTASIS,-SLIGHT -PIGMENT,-MINIMAL MARROW, FEMUR (FM) : -HYPOCELLULAR, MARROW,-SLIGHT HEART (HT) : -CARDIOMYOPATHY,-MINIMAL 2906 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1148 ANIMAL NUMBER: C92877 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 493.7 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 10:35 PROSECTOR: TROY ELLENBECKER RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL; COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN GENERAL INFORMATION (XX) : >NOTE:>ANIMAL IDENTIFICATION TRANSPONDER DAMAGED AT NECROPSY PITUITARY (PI) : -HYPERPLASIA,-SLIGHT MAMMARY, FEMALE (MF) : -HYPERPLASIA, GLANDULAR,-MODERATE CERVIX (CV) : -HYPERPLASIA, MUCINOUS,-MODERATE VAGINA (VA) : -HYPERPLASIA, MUCINOUS,-MODERATELY SEVERE CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MANDIBULAR (MN) : -HYPERPLASIA, LYMPHOPLASMACYTIC,MODERATELY SEVERE -HEMORRHAGE,-PRESENT 2907 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1149 ANIMAL NUMBER: C92877 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 493.7 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 10:35 PROSECTOR: TROY ELLENBECKER RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), HEMATO NEOPLASIA (HN) 2908 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1150 ANIMAL NUMBER: C92878 SEX: FEMALE DATE OF DEATH: 11/02/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 11/02/99 10:17 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 562 STUDY WEEK OF DEATH: 81 TERMINAL BODY WEIGHT: 510.6 GRAMS PROSECTOR: CHANESTA ACKER RECORDER: JOHN S. HALFORD PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); VENTRAL-THORACIC; WEEK 45; 100 X 100MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK 60; 35 X 35MM; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY SPLEEN (SP) : -LARGE; 48 X 13 X 9 MM ADRENAL, CORTEX (AC) : -LARGE; BILATERAL: EACH IS 9 X 7 X 5 MM HISTOPATHOLOGY LIVER (LI) : -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, PERIPORTAL,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, PELVIC,-MINIMAL -MINERALIZATION, TUBULAR,-MINIMAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATELY SEVERE ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC / HEMORRHAGIC,SEVERE MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,-SEVERE MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,-SEVERE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MINIMAL 2909 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1151 ANIMAL NUMBER: C92878 SEX: FEMALE DATE OF DEATH: 11/02/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 11/02/99 10:17 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 562 STUDY WEEK OF DEATH: 81 TERMINAL BODY WEIGHT: 510.6 GRAMS PROSECTOR: CHANESTA ACKER RECORDER: JOHN S. HALFORD PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 7 X 5 X 3 MM, DARK RED, TAN AND SOFT MAMMARY, FEMALE (MF) : -MASS(ES); LEFT INGUINAL REGION: SINGLE MASS, 50 X 40 X 28 MM, RED, DARK RED, TAN AND FIRM; ON SECTION EXUDES A DARK RED, CREAMY SEMIFLUID; COLLECTED INTACT; REGIONAL (INGUINAL) NORMAL (CAP 21) -MASS #2; MID CERVICAL REGION, EXTENDING TO VENTRAL THORACIC REGION: SINGLE MASS, 95 X 82 X 45 MM, RED, DARK RED, TAN, LOBULATED, VASCULAR AND FIRM; SAME ON CUT SURFACE; PORTIONS COLLECTED; REGIONAL LN (SEE MANDIBULAR) LN, MANDIBULAR (MN) : -LARGE; BILATERAL: EACH IS UP TO 10 X 8 X 3 MM (CAP 22) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT -B-FIBROADENOMA,-NEOPLASM PRESENT -B-ADENOMA,-NEOPLASM PRESENT >NOTE:>MASS #2 IS THE FIBROADENOMA CAUSE OF DEATH (CD) : -NEOPLASM, MAMMARY GLAND,-PRESENT LN, INGUINAL (IN) : >TISSUE MISSING LN, MANDIBULAR (MN) : -HYPERPLASIA, LYMPHOPLASMACYTIC,-SEVERE 2910 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1152 ANIMAL NUMBER: C92878 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/02/99 STUDY DAY OF DEATH: 562 STUDY WEEK OF DEATH: 81 TERMINAL BODY WEIGHT: 510.6 GRAMS DATE AND TIME OF NECROPSY: 11/02/99 10:17 PROSECTOR: CHANESTA ACKER RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -MALOCCLUSION; BILATERAL, UPPER INCISORS -ANIMAL THIN THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), TAIL (TI), LN, LUMBAr /i LIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 2911 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1153 ANIMAL NUMBER: C92879 SEX: FEMALE DATE OF DEATH: 01/24/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 01/24/00 12:09 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 645 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: 312.0 GRAMS PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); AXILLARY REGION-LEFT; WEEK 69; 10 X 10MM; VERIFIED AT NECROPSY: NOT OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); RECTUM; WEEK 92; 15 X 15MM; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; ATAXIC; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; HEAD TILT; LEFT; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 11 X 9 X 7 MM, DARK RED, TAN AND FIRM HISTOPATHOLOGY BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MODERATE -HYPERPLASIA, BILE DUCT,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, TUBULAR,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC / HEMORRHAGIC,MODERATE -PIGMENT,-SLIGHT HEART (HT) : -CARDIOMYOPATHY,-MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT 2912 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1154 ANIMAL NUMBER: C92879 SEX: FEMALE DATE OF DEATH: 01/24/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 01/24/00 12:09 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 645 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: 312.0 GRAMS PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS(ES); PERIANAL REGION EXTENDING TOWARDS LEFT INGUINAL REGION: SINGLE MASS, 33 X 22 X 15 MM, RED, TAN AND FIRM; ON SECTION EXUDES A TAN SEMIFLUID; COLLECTED INTACT; REGIONAL LN (INGUINAL) NOT IDENTIFIED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT -HYPERPLASIA, GLANDULAR,-MODERATELY SEVERE -B-ADENOMA,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 2913 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1155 ANIMAL NUMBER: C92879 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/24/00 STUDY DAY OF DEATH: 645 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: 312.0 GRAMS DATE AND TIME OF NECROPSY: 01/24/00 12:09 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON(CO), RECTUM (RE),SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 2914 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1156 ANIMAL NUMBER: C92880 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 260.1 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 10:53 PROSECTOR: JODI THIEREN RECORDER: KIMBERLY DURLAND POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) LF ADRENAL (AD0) RT ADRENAL (AD1) BRAIN (BR) LF KIDNEY (KD0) RT KIDNEY (KD1) LIVER (LI) LUNG (LU) LF OVARY (OV0) RT OVARY (OV1) SPLEEN (SP) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) UTERUS (UT) .0557 .0540 1.9264 1.0967 1.1309 8.6012 1.3026 .0727 .0804 .5075 .0060 .0076 1.1928 .0214 % .0208 % .7406 % .4216 % .4348 % 3.3069 % .5008 % .0280 % .0309 % .1951 % .0023 % .0029 % .4586 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0289 .0280 1.0000 .5693 .5871 4.4649 .6762 .0377 .0417 .2634 .0031 .0039 .6192 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: TWO DARK BROWN FOCI, EACH IS 1 MM IN DIAMETER LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-MINIMAL KIDNEY (KD) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT STOMACH, GL (ST) : >NOT REQUIRED TO BE EXAMINED FOR ANIMAL 2915 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1157 ANIMAL NUMBER: C92880 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 260.1 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 10:53 PROSECTOR: JODI THIEREN RECORDER: KIMBERLY DURLAND POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY UTERUS (UT) : -LUMEN FILLED WITH FLUID; BILATERAL: CLEAR FLUID GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN UTERUS (UT) : >NOT REQUIRED TO BE EXAMINED FOR ANIMAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV) 2916 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1158 ANIMAL NUMBER: C92881 SEX: FEMALE DATE OF DEATH: 04/21/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/21/00 11:08 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 488.2 GRAMS PROSECTOR: TODD ACKER RECORDER: TONE EVERTS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; MISSING; TEETH; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); INGUINAL REGION-LEFT; WEEK 99; 20 X 20MM; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -RED FOCUS(I)/AREA(S); DIAPHRAGMATIC SURFACE OF LEFT MEDIAN LOBE: SINGLE DARK RED AREA, 4 MM IN DIAMETER; SUNKEN WITH A STELLATE PATTERN (CAP 23) ADRENAL, CORTEX (AC) : -MASS(ES); ATTACHED TO LEFT GLAND: SINGLE MASS, 5 X 5 X 4 MM, DARK RED, BROWN, AND SOFT; COLLECTED INTACT; REGIONAL LN (SEE RENAL) OVARY (OV) : -CYST(S); BILATERAL: SINGLE CLEAR FLUID FILLED CYST ON EACH, LEFT IS 4 MM IN DIAMETER, RIGHT IS 2 MM IN DIAMETER HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -ANGIECTASIS,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-SLIGHT -HYPERTROPHY, CORTICAL CELL,-MODERATE -DEGENERATION, CYSTIC / HEMORRHAGIC,MODERATE -PIGMENT,-SLIGHT OVARY (OV) : >UNREMARKABLE 2917 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1159 ANIMAL NUMBER: C92881 SEX: FEMALE DATE OF DEATH: 04/21/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/21/00 11:08 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 488.2 GRAMS PROSECTOR: TODD ACKER RECORDER: TONE EVERTS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -RED FOCUS(I)/AREA(S); RIGHT ANTERIOR ASPECT: SINGLE DARK RED AND SLIGHTLY RAISED AREA, 4 X 3 X 1 MM MAMMARY, FEMALE (MF) : -MASS(ES); LEFT INGUINAL REGION: SINGLE MASS, 35 X 25 X 20 MM, DARK RED, RED, GRAY, FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED -MASS #2; RIGHT INGUINAL REGION: SINGLE MASS, 25 X 22 X 12, TAN AND SOFT; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED LN, RENAL (RL) : -MOTTLED; BILATERAL: RED AND TAN (CAP 26) THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATE HEART (HT) : -CARDIOMYOPATHY,-MINIMAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : -HYPERPLASIA,-MODERATELY SEVERE MAMMARY, FEMALE (MF) : -B-FIBROADENOMA,-NEOPLASM PRESENT -HYPERPLASIA, GLANDULAR,-MODERATE -B-ADENOMA,-NEOPLASM PRESENT >NOTE:>MASS #2 IS THE FIBROADENOMA CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, RENAL (RL) : -HYPERPLASIA, LYMPHOPLASMACYTIC,-SLIGHT LN, MANDIBULAR (MN) : -HYPERPLASIA, LYMPHOPLASMACYTIC,-SLIGHT 2918 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1160 ANIMAL NUMBER: C92881 SEX: FEMALE DATE OF DEATH: 04/21/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/21/00 11:08 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 488.2 GRAMS PROSECTOR: TODD ACKER RECORDER: TONE EVERTS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY LN, MANDIBULAR (MN) : -DIFFUSELY RED; RIGHT: DARK RED; COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -MALOCCLUSION; LEFT UPPER INCISOR -TEETH MISSING/BROKEN/WORN; RIGHT UPPER INCISOR: MISSING LN, MANDIBULAR (MN) : -HEMORRHAGE,-PRESENT 2919 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1161 ANIMAL NUMBER: C92881 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 488.2 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 11:08 PROSECTOR: TODD ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) (SM), (SG), 2920 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1162 ANIMAL NUMBER: C92882 SEX: FEMALE DATE OF DEATH: 04/21/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/21/00 11:14 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 432.9 GRAMS PROSECTOR: DAVID SCHUETTE RECORDER: TONE EVERTS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); AXILLARY REGION-LEFT; WEEK 69; 80 X 65MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); AXILLARY REGION-RIGHT; WEEK 100; 10 X 10MM; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) ADRENAL, CORTEX (AC) : -LARGE; LEFT: 7 X 7 X 4 MM (RIGHT IS 5 X 4 X 3 MM) -DIFFUSELY DARK; LEFT: DARK BROWN HISTOPATHOLOGY BRAIN (BR) : -HEMORRHAGE,-SLIGHT -I-CARCINOMA,-FROM PITUITARY LIVER (LI) : -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-SLIGHT -ANGIECTASIS,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, PELVIC,-SLIGHT SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATE -HYPERTROPHY, CORTICAL CELL,-MODERATE -DEGENERATION, CYSTIC / HEMORRHAGIC,MODERATE -HYPERPLASIA, CORTICAL,-MINIMAL -PIGMENT,-SLIGHT 2921 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1163 ANIMAL NUMBER: C92882 SEX: FEMALE DATE OF DEATH: 04/21/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/21/00 11:14 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 432.9 GRAMS PROSECTOR: DAVID SCHUETTE RECORDER: TONE EVERTS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY OVARY (OV) : -CYST(S); LEFT: SINGLE CLEAR FLUID FILLED CYST, 8 MM IN DIAMETER PITUITARY (PI) : -MASS(ES); 7 X 7 X 4 MM, DARK RED, TAN, AND SOFT STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: MULTIPLE DARK BROWN AREAS, 3 X 1 MM MAMMARY, FEMALE (MF) : -MASS(ES); LEFT AXILLARY REGION: SINGLE MASS, 90 X 75 X 40 MM (129.0 GRAMS), TAN, RED, LOBULATED, VASCULAR, AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (AXILLARY) NORMAL (CAP 22) OVARY (OV) : -CYST,-PRESENT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATE LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : -INFLAMMATION, ACUTE,-SLIGHT RECTUM (RE) : >TISSUE MISSING MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT -B-FIBROADENOMA,- 2 NEOPLASMS PRESENT -HYPERPLASIA, GLANDULAR,-MINIMAL 2922 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1164 ANIMAL NUMBER: C92882 SEX: FEMALE DATE OF DEATH: 04/21/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/21/00 11:14 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 432.9 GRAMS PROSECTOR: DAVID SCHUETTE RECORDER: TONE EVERTS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS #2; RIGHT AXILLARY REGION: SINGLE MASS, 35 X 30 X 15 MM, TAN AND FIRM; CUT SURFACE EXUDES A TAN CREAMY SEMIFLUID MATERIAL; COLLECTED INTACT; REGIONAL LN (AXILLARY) NORMAL (CAP 22) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN VAGINA (VA) : -LUMEN, EXUDATE,-PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2923 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1165 ANIMAL NUMBER: C92882 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 432.9 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 11:14 PROSECTOR: DAVID SCHUETTE RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), HEMATO NEOPLASIA (HN), LN, AXILLARY (AX) 2924 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1166 ANIMAL NUMBER: C92883 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/14/99 STUDY DAY OF DEATH: 604 STUDY WEEK OF DEATH: 87 TERMINAL BODY WEIGHT: 426.7 GRAMS DATE AND TIME OF NECROPSY: 12/14/99 13:51 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); INGUINAL REGION-RIGHT; WEEK 75; 20 X 20MM; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; ATAXIC; VERIFIED AT NECROPSY: NOT APPLICABLE >EXCRETION; NO FECES; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) HISTOPATHOLOGY EYE (EY) : -DYSTROPHY, CORNEAL IN EARLY STAGES, CONSISTS PRIMARILY OF FOCAL MINERALIZATION,-SLIGHT -INFLAMMATION, ACUTE,-SLIGHT BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-SLIGHT -HYPERPLASIA, BILE DUCT,-SLIGHT -FIBROSIS, PERIBILIARY,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MODERATE -MINERALIZATION, PELVIC,-MODERATE SPLEEN (SP) : -PIGMENT, INCREASED,-MINIMAL -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MINIMAL PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-SLIGHT 2925 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1167 ANIMAL NUMBER: C92883 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/14/99 STUDY DAY OF DEATH: 604 STUDY WEEK OF DEATH: 87 TERMINAL BODY WEIGHT: 426.7 GRAMS DATE AND TIME OF NECROPSY: 12/14/99 13:51 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY ADRENAL, CORTEX (AC) : -LARGE; LEFT: 6 MM IN DIAMETER (RIGHT IS 4 MM IN DIAMETER) LUNG (LU) : -LIGHT FOCUS(I)/AREA(S); LEFT LOBE AND RIGHT CAUDAL LOBE, ALONG CAUDAL MARGINS: MULTIPLE TAN FOCI, UP TO 2 MM IN DIAMETER PITUITARY (PI) : -MASS(ES); 13 X 11 X 9 MM, RED, DARK RED, GREY, TAN, LOBULATED AND SOFT STOMACH, GL (ST) : -EROSION/ULCERATION; MUCOSA, NEAR PYLORIC VALVE: SINGLE TAN ERODED AREA, 2 X 1 MM ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-MINIMAL -ANGIECTASIS,-MODERATELY SEVERE -HYPERTROPHY, CORTICAL CELL,-SLIGHT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MINIMAL LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL HEART (HT) : -CARDIOMYOPATHY,-SLIGHT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL STOMACH, GL (ST) : -EDEMA,-MODERATE -INFLAMMATION, ACUTE,-SLIGHT MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE -B-FIBROADENOMA,-NEOPLASM PRESENT -HYPERPLASIA, GLANDULAR,-MODERATE 2926 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1168 ANIMAL NUMBER: C92883 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/14/99 STUDY DAY OF DEATH: 604 STUDY WEEK OF DEATH: 87 TERMINAL BODY WEIGHT: 426.7 GRAMS DATE AND TIME OF NECROPSY: 12/14/99 13:51 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT INGUINAL REGION: SINGLE MASS, 55 X 36 X 19 MM, DARK RED, GREY, TAN, LOBULATED AND SOFT; SOME SECTIONS EXUDE A TAN, CREAMY SEMIFLUID MATERIAL AND OTHER SECTIONS EXUDE A DARK RED, MUCOID SEMIFLUID MATERIAL; COLLECTED INTACT; REGIONAL LN (INGUINAL) NOT IDENTIFIED -CYST(S); ALL REGIONS, DIFFUSE: MULTIPLE TAN, CREAMY SEMIFLUID FILLED CYSTS, UP TO 6 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; UPPER RIGHT INCISOR UTERUS (UT) : -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL CERVIX (CV) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT VAGINA (VA) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT HEMATO NEOPLASIA (HN) : -M-SARCOMA, HISTIOCYTIC,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 2927 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1169 ANIMAL NUMBER: C92883 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/14/99 STUDY DAY OF DEATH: 604 STUDY WEEK OF DEATH: 87 TERMINAL BODY WEIGHT: 426.7 GRAMS DATE AND TIME OF NECROPSY: 12/14/99 13:51 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -DISCHARGE-OCULAR; BILATERAL: DRY, RED MATERIAL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB) 2928 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1170 ANIMAL NUMBER: C92884 SEX: FEMALE DATE OF DEATH: 02/14/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 02/14/00 13:36 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 666 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: 328.8 GRAMS PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); VENTRAL-ABDOMINAL-RIGHT; WEEK 65; 25 X 30MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); VENTRAL-ABDOMINAL-RIGHT; WEEK 74; 30 X 25MM POSTERIOR; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK 79; 30 X 20MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); AXILLARY REGION-LEFT; WEEK 89; 20 X 15MM; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >EYES; PROTRUDING; EYES; VERIFIED AT NECROPSY: NOT OBSERVED >SKIN & PELAGE; PALE; ENTIRE BODY; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; SORE/SCAB; AXILLARY REGION-RIGHT; VERIFIED AT NECROPSY: NOT OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) HISTOPATHOLOGY BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT -HEMORRHAGE,-MODERATE LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -NECROSIS, COAGULATIVE,-MINIMAL -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, PELVIC,-MODERATE SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : -HYPERTROPHY, CORTICAL CELL,-MODERATELY SEVERE -DEGENERATION, CYSTIC / HEMORRHAGIC,SLIGHT -PIGMENT,-SLIGHT 2929 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1171 ANIMAL NUMBER: C92884 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/14/00 STUDY DAY OF DEATH: 666 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: 328.8 GRAMS DATE AND TIME OF NECROPSY: 02/14/00 13:36 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); SINGLE MASS, 11 X 9 X 5 MM, DARK RED, GRAY, AND SOFT MAMMARY, FEMALE (MF) : -MASS(ES); LEFT INGUINAL REGION: SINGLE MASS, 45 X 30 X 10 MM, TAN, GRAY, YELLOW, AND CYSTIC; ON SECTION EXUDES A TAN AND YELLOW CREAMY SEMIFLUID; COLLECTED; REGIONAL LN (INGUINAL) NORMAL (CAP 21) -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION -MASS #2; RIGHT INGUINAL REGION: SINGLE MASS, 40 X 30 X 25 MM, RED, TAN VASCULAR, AND FIRM; SOME AREAS EXUDE A BROWN, CREAMY SEMIFLUID; COLLECTED; REGIONAL LN (INGUINAL) NORMAL (CAP 21) MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,-MODERATE MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,-MODERATE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),SLIGHT HEART (HT) : -CARDIOMYOPATHY,-MINIMAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -M-CARCINOMA,- 3 NEOPLASMS PRESENT -CYST / GALACTOCELE,-PRESENT -B-FIBROADENOMA,-NEOPLASM PRESENT >NOTE:>MASS #1 IS THE FIBROADENOMA 2930 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1172 ANIMAL NUMBER: C92884 SEX: FEMALE DATE OF DEATH: 02/14/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 02/14/00 13:36 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 666 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: 328.8 GRAMS PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -CYST(S); ALL REGIONS, DIFFUSE: MULTIPLE TAN, YELLOW CREAMY SEMIFLUID FILLED CYSTS, UP TO 10 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION -MASS #3; RIGHT INGUINAL REGION, POSTERIOR TO MF MASS #2: SINGLE MASS, 45 X 40 X 20 MM, RED, DARK RED, TAN, LOBULATED, AND VASCULAR; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (INGUINAL) NORMAL -MASS #4; LEFT AXILLARY REGION: SINGLE MASS, 40 X 15 X 10 MM, RED, DARK RED, BROWN, DARK BROWN AND FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (AXILLARY) NORMAL (CAP 22); OVERLYING SKIN OF MASS HAS A SINGLE DARK BROWN, DRY, CRUSTED AREA, 20 X 10 MM; COLLECTED INTACT WITH MASS GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT LN, AXILLARY (AX) : -HYPERPLASIA, LYMPHOPLASMACYTIC,-SEVERE LN, INGUINAL (IN) : >TISSUE MISSING 2931 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1173 ANIMAL NUMBER: C92884 SEX: FEMALE DATE OF DEATH: 02/14/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 02/14/00 13:36 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 666 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: 328.8 GRAMS PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -STAINS - SKIN/HAIRCOAT; ENTIRE VENTRAL SURFACE: DARK RED AND BROWN THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 2932 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1174 ANIMAL NUMBER: C92885 SEX: FEMALE DATE OF DEATH: 04/21/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/21/00 7:48 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 452.2 GRAMS PROSECTOR: JOHN S. HALFORD RECORDER: LAURIE J. SCHULLER PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); INGUINAL REGION-LEFT; WEEK 99; 15 X 15MM; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -LIGHT FOCUS(I)/AREA(S); RIGHT MEDIAN LOBE ALONG MARGIN: SINGLE TAN AREA, 5 X 4 MM (CAP 22) -RED FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE DARK RED AREAS, UP TO 3 X 2 MM; COLLECTED ON ROUTINE SECTIONS HISTOPATHOLOGY BRAIN (BR) : >UNREMARKABLE LIVER (LI) : -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, PERIPORTAL,-MINIMAL -ANGIECTASIS,-SLIGHT -LIPIDOSIS, SUBCAPSULAR (TENSION LIPIDOSIS),-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SLIGHT -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : -HYPERTROPHY, CORTICAL CELL,-MINIMAL -DEGENERATION, CYSTIC / HEMORRHAGIC,MODERATELY SEVERE -PIGMENT,-SLIGHT ADRENAL, MEDULLA (MA) : -UNILATERALLY EXAMINED,-PRESENT 2933 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1175 ANIMAL NUMBER: C92885 SEX: FEMALE DATE OF DEATH: 04/21/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/21/00 7:48 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 452.2 GRAMS PROSECTOR: JOHN S. HALFORD RECORDER: LAURIE J. SCHULLER PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY OVARY (OV) : -CYST(S); BILATERAL: SINGLE CLEAR FLUID FILLED CYST ON EACH, LEFT IS 3 MM IN DIAMETER, RIGHT IS 8 MM IN DIAMETER PITUITARY (PI) : -MASS(ES); 6 X 5 X 4 MM, DARK RED, GRAY, AND SOFT STOMACH, GL (ST) : -THICKENED WALL; MUCOSA, DIFFUSE MAMMARY, FEMALE (MF) : -CYST(S); ALL REGIONS, DIFFUSE: MULTIPLE TAN, CREAMY SEMIFLUID FILLED CYSTS, UP TO 2 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION OVARY (OV) : -CYST,-PRESENT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),SLIGHT LUNG (LU) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL HEART (HT) : -CARDIOMYOPATHY,-MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : >UNREMARKABLE MAMMARY, FEMALE (MF) : -HYPERPLASIA, GLANDULAR,-MODERATELY SEVERE VAGINA (VA) : -HYPERPLASIA, MUCINOUS,-SEVERE CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2934 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1176 ANIMAL NUMBER: C92885 SEX: FEMALE DATE OF DEATH: 04/21/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/21/00 7:48 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 452.2 GRAMS PROSECTOR: JOHN S. HALFORD RECORDER: LAURIE J. SCHULLER PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY CAVITY, ABDOM (PC) : -MASS(ES); ADIPOSE TISSUE SURROUNDING LEFT KIDNEY: SINGLE MASS, 46 X 42 X 37 MM, RED, DARK RED, GRAY, TAN, VASCULAR, LOBULATED, AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT WITH LEFT KIDNEY; REGIONAL LN (RENAL) NOT IDENTIFIED; POSSIBLY CONSISTENT WITH MASS NOTED IN CLINICAL OBSERVATIONS GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN CAVITY, ABDOM (PC) : -M-LIPOSARCOMA,-NEOPLASM PRESENT 2935 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1177 ANIMAL NUMBER: C92885 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 452.2 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 7:48 PROSECTOR: JOHN S. HALFORD RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), HEMATO NEOPLASIA (HN) 2936 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1178 ANIMAL NUMBER: C92886 SEX: FEMALE DATE OF DEATH: 11/01/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 11/01/99 14:50 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 561 STUDY WEEK OF DEATH: 81 TERMINAL BODY WEIGHT: 676.1 GRAMS PROSECTOR: JODI THIEREN RECORDER: DEBBIE PIRKEL PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; SORE/SCAB; AXILLARY REGION-LEFT; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; SWOLLEN; VENTRAL ABDOMINAL; VERIFIED AT NECROPSY: OBSERVED >EYES; DISCOLORED/LIGHT RED; EYES; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; BLUE SKIN; VENTRAL ABDOMINAL; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -RED FOCUS(I)/AREA(S); DIAPHRAGMATIC SURFACE OF LEFT LATERAL LOBE: MULTIPLE RED AREAS, UP TO 2 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION KIDNEY (KD) : -DIFFUSELY LIGHT; BILATERAL: TAN THYMUS (TH) : -GELATINOUS; DIFFUSE: DARK RED AND SOFT PITUITARY (PI ) : -MASS(ES); 12 X 8 X 8 MM, RED, DARK RED, GRAY, TAN, FIRM, AND LOBULATED HISTOPATHOLOGY BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -HEMATOPOIESIS, EXTRAMEDULLARY,-SLIGHT KIDNEY (KD) : -MINERALIZATION, PELVIC,-SLIGHT SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : -HYPERTROPHY, CORTICAL CELL,-MINIMAL MARROW, FEMUR (FM) : -HYPERPLASIA, ERYTHROID, MARROW,-SLIGHT MARROW, STERNUM (SE) : -HYPERPLASIA, ERYTHROID, MARROW,-SLIGHT THYMUS (TH) : -EDEMA,-PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT 2937 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1179 ANIMAL NUMBER: C92886 SEX: FEMALE DATE OF DEATH: 11/01/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 11/01/99 14:50 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 561 STUDY WEEK OF DEATH: 81 TERMINAL BODY WEIGHT: 676.1 GRAMS PROSECTOR: JODI THIEREN RECORDER: DEBBIE PIRKEL PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY SKIN (SK) : -ABRASION(S)/ULCERATION(S); VENTRAL THORACIC REGION: SINGLE RED AND MOIST ULCERATED AREA, 5 X 3 MM (CAP 21); POSSIBLY CONSISTENT WITH (SORE, SCAB LEFT AXILLARY REGION) NOTED IN CLINICAL OBSERVATIONS MAMMARY, FEMALE (MF) : -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION UTERUS (UT) : -CYST(S); RIGHT, WITHIN LUMEN: SINGLE CLEAR FLUID FILLED CYST, 2 MM IN DIAMETER LN, MEDIASTINAL (ML) : -DIFFUSELY RED; DARK RED (CAP 24) SKIN (SK) : -ACANTHOSIS/HYPERKERATOSIS,-MODERATE -ULCERATION,-SLIGHT -FIBROSIS,-MODERATE -M-CARCINOMA, SQUAMOUS CELL,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT -HYPERPLASIA, GLANDULAR,-MODERATE UTERUS (UT) : -DILATATION,-MINIMAL VAGINA (VA) : -LUMEN, EXUDATE,-PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT LN, MEDIASTINAL (ML) : -HEMORRHAGE,-PRESENT -INFILTRATE, MACROPHAGE, PIGMENTED,SLIGHT ADIPOSE TISSUE (AT) : -M-LIPOSARCOMA,-NEOPLASM PRESENT 2938 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1180 ANIMAL NUMBER: C92886 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/01/99 STUDY DAY OF DEATH:: 561 STUDY WEEK OF DEATH: 81 TERMINAL BODY WEIGHT: 676.1 GRAMS DATE AND TIME OF NECROPSY: 11/01/99 14:50 PROSECTOR: JODI THIEREN RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY ADIPOSE TISSUE (AT) : -CYST(S); INVOLVING ALL ADIPOSE TISSUE THROUGHOUT ABDOMINAL CAVITY: MULTIPLE CYSTS, SOME ARE FILLED WITH CLEAR AND OILY FLUID AND SOME ARE FILLED WITH RED AND OILY FLUID, UP TO 25 MM IN DIAMETER (CAP 22); POSSIBLY CONSISTENT WITH (SWOLLEN ABDOMEN) NOTED IN CLINICAL OBSERVATIONS DIAPHRAGM (DP) : -MASS(ES); VISCERAL SURFACE: MULTIPLE MASSES, UP TO 4 MM IN DIAMETER, TAN FIRM, AND ADIPOSE TISSUE-LIKE (CAP 23); REGIONAL LN (SEE MEDIASTINAL) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN DIAPHRAGM (DP) : -I-LIPOSARCOMA,-NEOPLASM PRESENT 2939 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1181 ANIMAL NUMBER: C92886 SEX: FEMALE DATE OF DEATH: 11/01/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 11/01/99 14:50 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 561 STUDY WEEK OF DEATH: 81 TERMINAL BODY WEIGHT: 676.1 GRAMS PROSECTOR: JODI THIEREN RECORDER: DEBBIE PIRKEL PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOt /f OOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), CERVIX (CV), HEMATO NEOPLASIA (HN) 2940 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1182 ANIMAL NUMBER: C92887 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 275.7 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 10:02 PROSECTOR: LAURIE J. SCHULLER RECORDER: KIMBERLY DURLAND POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) LF ADRENAL (AD0) RT ADRENAL (AD1) BRAIN (BR) LF KIDNEY (KD0) RT KIDNEY (KD1) LIVER (LI) LUNG (LU) LF OVARY (OV0) RT OVARY (OV1) SPLEEN (SP) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) UTERUS (UT) .0376 .0317 1.9947 1.2699 1.2956 9.7028 1.5519 .0775 .0731 .4733 .0050 .0075 .7463 .0136 % .0115 % .7235 % .4606 % .4699 % 3.5193 % .5629 % .0281 % .0265 % .1717 % .0018 % .0027 % .2707 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0188 .0159 1.0000 .6366 .6495 4.8643 .7780 .0389 .0366 .2373 .0025 .0038 .3741 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED KIDNEY (KD) : -CYST(S); RIGHT CORTICAL SURFACE EXTENDING TO CORTEX: SINGLE CLEAR FLUID FILLED CYST, 3 MM IN DIAMETER LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-SLIGHT -NECROSIS, INDIVIDUAL HEPATOCYTE,MINIMAL KIDNEY (KD) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2941 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1183 ANIMAL NUMBER: C92887 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 275.7 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 10:02 PROSECTOR: LAURIE J. SCHULLER RECORDER: KIMBERLY DURLAND POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV) 2942 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1184 ANIMAL NUMBER: C92888 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/24/99 STUDY DAY OF DEATH: 553 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: 545.7 GRAMS DATE AND TIME OF NECROPSY: 10/24/99 7:52 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >EYES; RED DISCHARGE; EYE-RIGHT; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; HEAD TILT; RIGHT; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -VACUOLATION, HEPATOCELLULAR, MIDZONAL / CENTRILOBULAR,-SLIGHT -HYPERPLASIA, BILE DUCT,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, PERIPORTAL,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, PELVIC,-SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE PANCREAS (PA) : -HYPERPLASIA, ISLET CELLS,-SLIGHT -INFILTRATION, FAT, INTERSTITIAL,MODERATE ADRENAL, CORTEX (AC) : -PIGMENT,-MINIMAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MINIMAL 2943 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1185 ANIMAL NUMBER: C92888 SEX: FEMALE DATE OF DEATH: 10/24/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 10/24/99 7:52 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 553 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: 545.7 GRAMS PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 15 X 5 X 5 MM, RED, DARK RED, TAN, AND SOFT VAGINA (VA) : -SEMIFLUID MATERIAL; LIGHT GREEN, CREAMY SEMIFLUID GENERAL COMMENT (GC) -BLOOD FILM TAKEN -DISCHARGE-OCULAR; CRUSTED -ANIMAL OBESE : RIGHT: DARK RED AND HEART (HT) : -CARDIOMYOPATHY,-MINIMAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT VAGINA (VA) : -LUMEN, EXUDATE,-PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 2944 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1186 ANIMAL NUMBER: C92888 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/24/99 STUDY DAY OF DEATH: 553 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: 545.7 GRAMS DATE AND TIME OF NECROPSY: 10/24/99 7:52 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), HEMATO NEOPLASIA (HN) 2945 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1187 ANIMAL NUMBER: C92889 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 367.3 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 9:49 PROSECTOR: JOHN S. HALFORD RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) LF ADRENAL (AD0) RT ADRENAL (AD1) BRAIN (BR) LF KIDNEY (KD0) RT KIDNEY (KD1) LIVER (LI) LUNG (LU) LF OVARY (OV0) RT OVARY (OV1) SPLEEN (SP) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) UTERUS (UT) .0321 .0277 1.9141 1.1397 1.2457 9.4841 1.9131 .0753 .0475 .4549 .0284 .0181 .6258 .0087 % .0075 % .5211 % .3103 % .3392 % 2.5821 % .5209 % .0205 % .0129 % .1238 % .0077 % .0049 % .1704 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0168 .0145 1.0000 .5954 .6508 4.9549 .9995 .0393 .0248 .2377 .0148 .0095 .3269 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-MINIMAL -INFLAMMATION, SUBACUTE,-SLIGHT KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATELY SEVERE 2946 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1188 ANIMAL NUMBER: C92889 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 367.3 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 9:49 PROSECTOR: JOHN S. HALFORD RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -DARK FOCUS(I)/AREA(S); RIGHT POSTERIOR ASPECT: SINGLE DARK FOCUS, 2 MM IN DIAMETER GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN ADRENAL, CORTEX (AC) : -UNILATERALLY EXAMINED,-PRESENT -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL ADRENAL, MEDULLA (MA) : -UNILATERALLY EXAMINED,-PRESENT HEART (HT) : -CARDIOMYOPATHY,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT -ANGIECTASIS,-PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2947 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1189 ANIMAL NUMBER: C92889 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 367.3 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 9:49 PROSECTOR: JOHN S. HALFORD RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) (CE), 2948 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1190 ANIMAL NUMBER: C92890 SEX: FEMALE DATE OF DEATH: 04/21/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/21/00 9:55 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 510.2 GRAMS PROSECTOR: DAVID SCHUETTE RECORDER: TONE EVERTS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY KIDNEY (KD) : -MASS(ES); RIGHT CORTICAL SURFACE: SINGLE MASS, 5 MM IN DIAMETER, TAN AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (RENAL) NORMAL (CAP 22) ADRENAL, CORTEX (AC) : -LARGE; RIGHT: 7 X 7 X 5 MM (LEFT IS 5 X 4 X 3 MM) HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -M-CARCINOMA, TUBULAR CELL,-NEOPLASM PRESENT SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE PANCREAS (PA) : -INFILTRATION, FAT, INTERSTITIAL,SLIGHT ADRENAL, CORTEX (AC) : -HYPERTROPHY, CORTICAL CELL,-MODERATELY SEVERE -DEGENERATION, CYSTIC / HEMORRHAGIC,MODERATELY SEVERE -PIGMENT,-MINIMAL OVARY (OV) : -CYST,-PRESENT 2949 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1191 ANIMAL NUMBER: C92890 SEX: FEMALE DATE OF DEATH: 04/21/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/21/00 9:55 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 510.2 GRAMS PROSECTOR: DAVID SCHUETTE RECORDER: TONE EVERTS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY OVARY (OV) : -CYST(S); RIGHT: SINGLE CLEAR FLUID FILLED CYST, 5 MM IN DIAMETER PITUITARY (PI) : -RED FOCUS(I)/AREA(S); RIGHT MID ASPECT: SINGLE DARK RED AREA, 2 MM IN DIAMETER COLON (CO) : -DIVERTICULUM(A); NEAR JUNCTION OF CECUM: SINGLE BROWN DIVERTICULUM, 5 X 5 X 3 MM; COLLECTED ON ROUTINE SECTION MAMMARY, FEMALE (MF) : -THICKENED; ALL REGIONS DIFFUSE: TAN; COLLECTED ON ROUTINE SECTION BONE, STERNUM (SB) : -HYPERPLASIA, CARTILAGINOUS,-MODERATE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MINIMAL LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL PITUITARY (PI) : -HYPERPLASIA,-MODERATE COLON (CO) : -DIVERTICULUM,-PRESENT MAMMARY, FEMALE (MF) : -HYPERPLASIA, GLANDULAR,-MODERATE CERVIX (CV) : -HYPERPLASIA, MUCINOUS,-SLIGHT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, RENAL (RL) : -HEMORRHAGE,-PRESENT 2950 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1192 ANIMAL NUMBER: C92890 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 510.2 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 9:55 PROSECTOR: DAVID SCHUETTE RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -MALOCCLUSION; BILATERAL UPPER INCISORS LN, RENAL (RL) : -INFILTRATE, MACROPHAGE, PIGMENTED,SLIGHT 2951 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1193 ANIMAL NUMBER: C92890 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 510.2 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 9:55 PROSECTOR: DAVID SCHUETTE RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) 2952 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1194 ANIMAL NUMBER: C92891 SEX: FEMALE DATE OF DEATH: 12/01/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 12/01/99 8:08 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 591 STUDY WEEK OF DEATH: 85 TERMINAL BODY WEIGHT: 314.7 GRAMS PROSECTOR: DONIA MEYER RECORDER: DONIA MEYER PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; HEAD TILT; LEFT; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; RECUMBENT; LATERAL; VERIFIED AT NECROPSY: NOT APPLICABLE >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >RESPIRATION; AUDIBLE; VERIFIED AT NECROPSY: NOT APPLICABLE >RESPIRATION; IRREGULAR; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -RED FOCUS(I)/AREA(S); RIGHT MEDIAN LOBE: SINGLE DARK RED FOCUS, 4 MM IN DIAMETER, SUNKEN WITH A STELLATE PATTERN; COLLECTED ON ROUTINE SECTION PITUITARY (PI) : -MASS(ES); 10 X 10 X 8 MM, DARK RED AND SOFT HISTOPATHOLOGY BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-SLIGHT -ANGIECTASIS,-MINIMAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATE -PIGMENT,-SLIGHT ADRENAL, MEDULLA (MA) : -B-PHEOCHROMOCYTOMA,-NEOPLASM PRESENT LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,SLIGHT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT 2953 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1195 ANIMAL NUMBER: C92891 SEX: FEMALE DATE OF DEATH: 12/01/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 12/01/99 8:08 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 591 STUDY WEEK OF DEATH: 85 TERMINAL BODY WEIGHT: 314.7 GRAMS PROSECTOR: DONIA MEYER RECORDER: DONIA MEYER PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY LN, MEDIASTINAL (ML) : -DIFFUSELY RED; DARK RED (CAP 22) LN, MANDIBULAR (MN) : -MOTTLED; BILATERAL: DARK RED AND TAN (CAP 21) CAVITY, THORACIC (TA) : -MASS(ES); ATTACHED TO PLEURA, BETWEEN VERTEBRAE AND RIBS: SINGLE MASS, 20 X 10 X 10 MM, RED, TAN, AND SOFT; SAME ON CUT SURFACE; COLLECTED INTACT WITH SPINAL CORDD; REGIONAL LN (SEE MEDIASTINAL) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN MAMMARY, FEMALE (MF) : -HYPERPLASIA, GLANDULAR,-SLIGHT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT LN, MEDIASTINAL (ML) : -HEMORRHAGE,-PRESENT -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL LN, MANDIBULAR (MN) : -HYPERPLASIA, LYMPHOPLASMACYTIC,MODERATE -HEMORRHAGE,-PRESENT CAVITY, THORACIC (TA) : -B-HIBERNOMA,-NEOPLASM PRESENT 2954 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1196 ANIMAL NUMBER: C92891 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/01/99 STUDY DAY OF DEATH: 591 STUDY WEEK OF DEATH: 85 TERMINAL BODY WEIGHT: 314.7 GRAMS DATE AND TIME OF NECROPSY: 12/01/99 8:08 PROSECTOR: DONIA MEYER RECORDER: DONIA MEYER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 2955 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1197 ANIMAL NUMBER: C92892 SEX: FEMALE DATE OF DEATH: 04/20/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/20/00 8:50 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 688.1 GRAMS PROSECTOR: TONY HANFELD RECORDER: JERALD W. JOHNSON PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; OBES; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) HISTOPATHOLOGY BRAIN (BR) : >UNREMARKABLE LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-SLIGHT -HYPERPLASIA, BILE DUCT,-MINIMAL -FIBROSIS, PERIBILIARY,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, PELVIC,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE PANCREAS (PA) : -INFILTRATION, FAT, INTERSTITIAL,MINIMAL ADRENAL, CORTEX (AC) : -PIGMENT,-MINIMAL 2956 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1198 ANIMAL NUMBER: C92892 SEX: FEMALE DATE OF DEATH: 04/20/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/20/00 8:50 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 688.1 GRAMS PROSECTOR: TONY HANFELD RECORDER: JERALD W. JOHNSON PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 6 X 6 X 4 MM, RED, DARK RED AND SOFT MAMMARY, FEMALE (MF) : -MASS(ES); LEFT INGUINAL REGION: SINGLE MASS, 55 X 38 X 14 MM, RED, DARK RED, TAN AND SOFT; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -ANIMAL OBESE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),SLIGHT LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-MODERATE -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -B-FIBROADENOMA,-NEOPLASM PRESENT -HYPERPLASIA, GLANDULAR,-MODERATE VAGINA (VA) : -HYPERPLASIA, MUCINOUS,-MODERATE CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2957 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1199 ANIMAL NUMBER: C92892 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 688.1 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 8:50 PROSECTOR: TONY HANFELD RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), HEMATO NEOPLASIA (HN) 2958 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1200 ANIMAL NUMBER: C92893 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/20/99 STUDY DAY OF DEATH: 610 STUDY WEEK OF DEATH: 88 TERMINAL BODY WEIGHT: 367.4 GRAMS DATE AND TIME OF NECROPSY: 12/20/99 15:24 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); AXILLARY REGION-RIGHT; WEEK 74; 10 X 10MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); VENTRAL-THORACIC-LEFT; WEEK 74; 40 X 40MM; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; VENTRALTHORACIC-LEFT; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; HUNCHED; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) SPLEEN (SP) : -LARGE; 50 X 17 X 10 MM HISTOPATHOLOGY BRAIN (BR) : >UNREMARKABLE LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL -HEMATOPOIESIS, EXTRAMEDULLARY,-SLIGHT -HYPERPLASIA, BILE DUCT,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, PELVIC,-MINIMAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATELY SEVERE ADRENAL, CORTEX (AC) : -HYPERTROPHY, CORTICAL CELL,-MODERATE -DEGENERATION, CYSTIC / HEMORRHAGIC,MODERATELY SEVERE -PIGMENT,-SLIGHT OVARY (OV) : -B-GRANULOSA/THECA CELL TUMOR,-NEOPLASM PRESENT 2959 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1201 ANIMAL NUMBER: C92893 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/20/99 STUDY DAY OF DEATH: 610 STUDY WEEK OF DEATH: 88 TERMINAL BODY WEIGHT: 367.4 GRAMS DATE AND TIME OF NECROPSY: 12/20/99 15:24 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 7 X 6 X 4 MM, DARK RED, TAN, GRAY, AND SOFT MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT AXILLARY REGION: SINGLE MASS, 20 X 10 X 10 MM, DARK RED, TAN, AND FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (SEE AXILLARY) -MASS #2; LEFT AXILLARY REGION: SINGLE MASS, 55 X 50 X 40 MM, DARK RED, TAN, AND FIRM; SOME SECTIONS CONTAIN A TAN CASEOUS SEMISOLID; COLLECTED; REGIONAL LN (SEE AXILLARY); OVERLYING SKIN HAS A SINGLE DARK RED AND TAN ULCERATION, 30 MM IN DIAMETER; COLLECTED MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,MODERATELY SEVERE MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,MODERATELY SEVERE LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -M-CARCINOMA,-NEOPLASM PRESENT -CYST / GALACTOCELE,-PRESENT >NOTE:>MASS #1 IS AN ENLARGED SUBCUTANEOUS LYMPH NODE AND GALACTOCELES 2960 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1202 ANIMAL NUMBER: C92893 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/20/99 STUDY DAY OF DEATH: 610 STUDY WEEK OF DEATH: 88 TERMINAL BODY WEIGHT: 367.4 GRAMS DATE AND TIME OF NECROPSY: 12/20/99 15:24 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -CYST(S); ALL REGIONS, DIFFUSE: MULTIPLE TAN AND CREAMY SEMIFLUID FILLED CYSTS, UP TO 5 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION UTERUS (UT) : -THICKENED WALL; BILATERAL: DIFFUSE LN, AXILLARY (AX) : -LARGE; LEFT: UP TO 15 X 8 X 5 MM (RIGHT IS UP TO 7 X 6 X 3 MM) (CAP 23) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL, UPPER INCISORS UTERUS (UT) : >UNREMARKABLE CAUSE OF DEATH (CD) : -NEOPLASM, MAMMARY GLAND,-PRESENT LN, AXILLARY (AX) : -HYPERPLASIA, LYMPHOPLASMACYTIC,MODERATELY SEVERE 2961 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1203 ANIMAL NUMBER: C92893 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/20/99 STUDY DAY OF DEATH: 610 STUDY WEEK OF DEATH: 88 TERMINAL BODY WEIGHT: 367.4 GRAMS DATE AND TIME OF NECROPSY: 12/20/99 15:24 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 2962 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1204 ANIMAL NUMBER: C92894 SEX: FEMALE DATE OF DEATH: 04/20/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/20/99 10:01 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 335.4 GRAMS PROSECTOR: GREGORY CODDINGTON RECORDER: TONY HANFELD PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) LF ADRENAL (AD0) RT ADRENAL (AD1) BRAIN (BR) LF KIDNEY (KD0) RT KIDNEY (KD1) LIVER (LI) LUNG (LU) LF OVARY (OV0) RT OVARY (OV1) SPLEEN (SP) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) UTERUS (UT) .1249 .0492 2.1413 1.2732 1.3419 10.7609 1.7755 .0539 .0463 .4753 .0096 .0161 1.4655 .0372 % .0147 % .6384 % .3796 % .4001 % 3.2084 % .5294 % .0161 % .0138 % .1417 % .0029 % .0048 % .4369 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0583 .0230 1.0000 .5946 .6267 5.0254 .8292 .0252 .0216 .2220 .0045 .0075 .6844 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED ADRENAL, CORTEX (AC) : -LARGE; LEFT: 7 MM IN DIAMETER (FOR COMPARISON, RIGHT IS 4 MM IN DIAMETER) LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -NECROSIS, INDIVIDUAL HEPATOCYTE,MINIMAL KIDNEY (KD) : -MINERALIZATION, TUBULAR,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : -HEMORRHAGE,-PRESENT 2963 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1205 ANIMAL NUMBER: C92894 SEX: FEMALE DATE OF DEATH: 04/20/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/20/99 10:01 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 335.4 GRAMS PROSECTOR: GREGORY CODDINGTON RECORDER: TONY HANFELD PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY ESOPHAGUS (ES) : >NOTE:>TRIMMING COMMENT: NOT FOUND AT TRIMMING GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MINIMAL LUNG (LU) : -HEMORRHAGE,-PRESENT ESOPHAGUS (ES) : >TISSUE MISSING CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2964 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1206 ANIMAL NUMBER: C92894 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 335.4 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 10:01 PROSECTOR: GREGORY CODDINGTON RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 2965 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1207 ANIMAL NUMBER: C92895 SEX: FEMALE DATE OF DEATH: 04/20/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/20/00 11:10 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 458.9 GRAMS PROSECTOR: TONY HANFELD RECORDER: JERALD W. JOHNSON PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); AXILLARY REGION-LEFT; WEEK 87; 45 X 40MM; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, PELVIC,-SLIGHT SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATELY SEVERE PANCREAS (PA) : -INFILTRATION, FAT, INTERSTITIAL,MINIMAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATE -HYPERTROPHY, CORTICAL CELL,-MINIMAL -HYPERPLASIA, CORTICAL,-MINIMAL -PIGMENT,-SLIGHT OVARY (OV) : -CYST,-PRESENT 2966 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1208 ANIMAL NUMBER: C92895 SEX: FEMALE DATE OF DEATH: 04/20/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/20/00 11:10 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 458.9 GRAMS PROSECTOR: TONY HANFELD RECORDER: JERALD W. JOHNSON PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY OVARY (OV) : -CYST(S); LEFT: SINGLE CLEAR FLUID FILLED CYST, 3 MM IN DIAMETER MAMMARY, FEMALE (MF) : -MASS(ES); LEFT AXILLARY REGION: SINGLE MASS, 50 X 40 X 14 MM, RED, DARK RED, TAN AND SOFT; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (AXILLARY) NORMAL (CAP 22) -THICKENED; ALL REGIONS, DIFFUSE: COLLECTED ON ROUTINE SECTION GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATE PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -B-FIBROADENOMA,-NEOPLASM PRESENT -HYPERPLASIA, GLANDULAR,-MODERATE CERVIX (CV) : -HYPERPLASIA, MUCINOUS,-MODERATE VAGINA (VA) : -HYPERPLASIA, MUCINOUS,-SEVERE CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2967 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1209 ANIMAL NUMBER: C92895 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 458.9 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 11:10 PROSECTOR: TONY HANFELD RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), HEMATO NEOPLASIA (HN), LN, AXILLARY (AX) (SE), 2968 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1210 ANIMAL NUMBER: C92896 SEX: FEMALE DATE OF DEATH: 04/19/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/19/00 11:48 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 528.7 GRAMS PROSECTOR: DAVID SCHUETTE RECORDER: LAURIE J. SCHULLER PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); INGUINAL REGION-RIGHT; WEEK 76; 60 X 40MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); VENTRAL-THORACIC; WEEK 104; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL LIVER-OIL RED O (LI0) : -VACUOLES, OIL RED O-POSITIVE, HEPATOCELLULAR,-SLIGHT KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL -MINERALIZATION, TUBULAR,-MINIMAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE PANCREAS (PA) : -B-ADENOMA, ISLET CELL,-NEOPLASM PRESENT ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATE -PIGMENT,-SLIGHT OVARY (OV) : -CYST,-PRESENT MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,-MODERATE 2969 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1211 ANIMAL NUMBER: C92896 SEX: FEMALE DATE OF DEATH: 04/19/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/19/00 11:48 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 528.7 GRAMS PROSECTOR: DAVID SCHUETTE RECORDER: LAURIE J. SCHULLER PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 7 X 7 X 3 MM, TAN AND SOFT MAMMARY, FEMALE (MF) : -MASS(ES); BILATERAL VENTRAL CERVICAL REGIONS: MULTIPLE MASSES, UP TO 10 X 9 X 9 MM, ALL ARE TAN AND FIRM; SAME ON CUT SURFACES; REGIONAL LN (AXILLARY) RIGHT (NORMAL) (CAP 23) LEFT (NOT IDENTIFIED) -MASS #2; RIGHT INGUINAL REGION: SINGLE MASS, 75 X 55 X 35 MM, DARK BROWN, TAN, SOFT, LOBULATED, AND VASCULAR; CUT SURFACE EXUDES A DARK BROWN FLUID; COLLECTED; REGIONAL LN (INGUINAL) NORMAL (CAP 24) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,-SLIGHT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY) MODERATE HEART (HT) : -CARDIOMYOPATHY,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -M-CARCINOMA,-NEOPLASM PRESENT -CYST / GALACTOCELE,-PRESENT -B-ADENOMA,-NEOPLASM PRESENT >NOTE:>MASS #1 IS THE ADENOMA CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2970 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1212 ANIMAL NUMBER: C92896 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 528.7 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 11:48 PROSECTOR: DAVID SCHUETTE RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN), LN, AXILLARY (AX), LN, INGUINAL (IN) 2971 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1213 ANIMAL NUMBER: C92897 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 296.7 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 7:46 PROSECTOR: TONY HANFELD RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >EYES; SQUINTED; PERI-ORBITAL; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE DARK RED AREAS, UP TO 4 X 3 MM; COLLECTED ON ROUTINE SECTIONS HISTOPATHOLOGY BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, PERIPORTAL,-SLIGHT LIVER-OIL RED O (LI0) : -VACUOLES, OIL RED O-POSITIVE, HEPATOCELLULAR,-MINIMAL KIDNEY (KD) : -MINERALIZATION, TUBULAR,-MINIMAL -INFLAMMATION, SUPPURATIVE, PELVIC,MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE PANCREAS (PA) : -B-ADENOMA, ISLET CELL,-NEOPLASM PRESENT 2972 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1214 ANIMAL NUMBER: C92897 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 296.7 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 7:46 PROSECTOR: TONY HANFELD RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 10 X 10 X 8 MM, RED, DARK RED, TAN, LOBULATED, AND FIRM VAGINA (VA) : -SEMIFLUID MATERIAL; WITHIN LUMEN: GREEN MUCOID MATERIAL GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -ANIMAL THIN ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-MINIMAL -ANGIECTASIS,-SLIGHT -PIGMENT,-SLIGHT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATE THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT -HYPERPLASIA, GLANDULAR,-SLIGHT URINARY BLADDER (UB) : -DIVERTICULUM,-PRESENT UTERUS (UT) : -DILATATION,-SLIGHT VAGINA (VA) : -LUMEN, EXUDATE,-PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2973 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1215 ANIMAL NUMBER: C92897 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 296.7 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 7:46 PROSECTOR: TONY HANFELD RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUm /s KULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), CERVIX (CV), HEMATO NEOPLASIA (HN) (SG), 2974 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1216 ANIMAL NUMBER: C92898 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 284.2 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 10:52 PROSECTOR: TROY ELLENBECKER RECORDER: KIMBERLY DURLAND POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) LF ADRENAL (AD0) RT ADRENAL (AD1) BRAIN (BR) LF KIDNEY (KD0) RT KIDNEY (KD1) LIVER (LI) LUNG (LU) LF OVARY (OV0) RT OVARY (OV1) SPLEEN (SP) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) UTERUS (UT) .0386 .0332 2.0817 .9699 1.0336 8.3705 1.6120 .0858 .0637 .4762 .0083 .0080 .4686 .0136 % .0117 % .7325 % .3413 % .3637 % 2.9453 % .5672 % .0302 % .0224 % .1676 % .0029 % .0028 % .1649 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0185 .0159 1.0000 .4659 .4965 4.0210 .7744 .0412 .0306 .2288 .0040 .0038 .2251 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL KIDNEY (KD) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -REGENERATION, TUBULAR EPITHELIUM,MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2975 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1217 ANIMAL NUMBER: C92898 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 284.2 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 10:52 PROSECTOR: TROY ELLENBECKER RECORDER: KIMBERLY DURLAND POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV) 2976 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1218 ANIMAL NUMBER: C92899 SEX: FEMALE DATE OF DEATH: 04/20/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/02/99 9:15 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 435.3 GRAMS PROSECTOR: KEVIN BILLINGS RECORDER: TONY HANFELD PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) LF ADRENAL (AD0) RT ADRENAL (AD1) BRAIN (BR) LF KIDNEY (KD0) RT KIDNEY (KD1) LIVER (LI) LUNG (LU) LF OVARY (OV0) RT OVARY (OV1) SPLEEN (SP) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) UTERUS (UT) .0446 .0404 2.1677 1.4659 1.5078 13.0348 2.0543 .0505 .8172 .6569 .0086 .0095 1.0497 .0102 % .0093 % .4980 % .3368 % .3464 % 2.9944 % .4719 % .0116 % .1877 % .1509 % .0020 % .0022 % .2411 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0206 .0186 1.0000 .6762 .6956 6.0132 .9477 .0233 .3770 .3030 .0040 .0044 .4842 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >SKIN & PELAGE; SORE/SCAB; PAW-HINDLEFT; VERIFIED AT NECROPSY: NOT OBSERVED LIVER (LI) : -VACUOLATION, HEPATOCELLULAR,-MINIMAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATELY SEVERE ADRENAL, CORTEX (AC) : -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL -DEGENERATION, CYSTIC / HEMORRHAGIC,MINIMAL 2977 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1219 ANIMAL NUMBER: C92899 SEX: FEMALE DATE OF DEATH: 04/20/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/02/99 9:15 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 435.3 GRAMS PROSECTOR: KEVIN BILLINGS RECORDER: TONY HANFELD PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY OVARY (OV) : -CYST(S); RIGHT: SINGLE CLEAR FLUID FILLED CYST, 8 MM IN DIAMETER MAMMARY, FEMALE (MF) : -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN OVARY (OV) : -CYST,-PRESENT THYMUS (TH) : -HEMORRHAGE,-PRESENT MAMMARY, FEMALE (MF) : -HYPERPLASIA, GLANDULAR,-SLIGHT -SECRETORY MATERIAL,-PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2978 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1220 ANIMAL NUMBER: C92899 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 435.3 GRAMS DATE AND TIME OF NECROPSY: 04/02/99 9:15 PROSECTOR: KEVIN BILLINGS RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) (DU), 2979 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1221 ANIMAL NUMBER: C92900 SEX: FEMALE DATE OF DEATH: 09/05/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 09/05/99 13:22 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DEATH: 504 STUDY WEEK OF DEATH: 72 TERMINAL BODY WEIGHT: NOT ENTERED PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; MISSING; TEETH; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) ADRENAL, CORTEX (AC) : -LARGE; LEFT: 6 MM IN DIAMETER (RIGHT IS 3 MM IN DIAMETER) OVARY (OV) : -CYST(S); BILATERAL: SINGLE CLEAR FLUID FILLED CYST ON EACH, EACH IS 3 MM IN DIAMETER PITUITARY (PI) : -MASS(ES); 10 X 6 X 5 MM, DARK RED AND FIRM HISTOPATHOLOGY EYE (EY) : -DYSTROPHY, CORNEAL IN EARLY STAGES, CONSISTS PRIMARILY OF FOCAL MINERALIZATION,-SLIGHT BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT KIDNEY (KD) : -MINERALIZATION, PELVIC,-SLIGHT -MINERALIZATION, TUBULAR,-MINIMAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SLIGHT ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC / HEMORRHAGIC,SEVERE OVARY (OV) : -CYST,-PRESENT THYMUS (TH) : -CYST,-PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT 2980 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1222 ANIMAL NUMBER: C92900 SEX: FEMALE DATE OF DEATH: 09/05/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 09/05/99 13:22 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DEATH: 504 STUDY WEEK OF DEATH: 72 TERMINAL BODY WEIGHT: NOT ENTERED PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL -LARGE; BILATERAL: EACH IS 8 X 4 X 2 MM GENERAL COMMENT (GC) : -MALOCCLUSION; BILATERAL, LOWER INCISORS -TEETH MISSING/BROKEN/WORN; MISSING, LEFT UPPER INCISOR CECUM (CE) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT LN, MANDIBULAR (MN) : -HYPERPLASIA, LYMPHOPLASMACYTIC,MODERATELY SEVERE 2981 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1223 ANIMAL NUMBER: C92900 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 09/05/99 STUDY DAY OF DEATH: 504 STUDY WEEK OF DEATH: 72 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 09/05/99 13:22 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LIVER (LI), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), COLON RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) (CO), 2982 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1224 ANIMAL NUMBER: C92901 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/22/99 STUDY DAY OF DEATH: 551 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: 268.1 GRAMS DATE AND TIME OF NECROPSY: 10/22/99 15:15 PROSECTOR: JARED FISHEL RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); RECTUM; WEEK 76; 10 X 10MM; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; HEAD TILT; LEFT; VERIFIED AT NECROPSY: NOT APPLICABLE >EYES; RED DISCHARGE; EYE-LEFT; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; ATAXIC; VERIFIED AT NECROPSY: NOT APPLICABLE >DISCHARGE; NASAL; RED IN COLOR; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 12 X 8 X 6 MM, DARK RED, GRAY, SOFT AND LOBULATED HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATE LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE 2983 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1225 ANIMAL NUMBER: C92901 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/22/99 STUDY DAY OF DEATH: 551 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: 268.1 GRAMS DATE AND TIME OF NECROPSY: 10/22/99 15:15 PROSECTOR: JARED FISHEL RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -CYST(S); RIGHT PERIANAL REGION: SINGLE GREEN SEMIFLUID FILLED CYST, 11 MM IN DIAMETER; COLLECTED; POSSIBLY CONSISTENT WITH MASS (RECTUM) NOTED IN CLINICAL OBSERVATIONS UTERUS (UT) : -CYST(S); LEFT HORN, WITHIN WALL: MULTIPLE CLEAR FLUID FILLED CYSTS; UP TO 3 MM IN DIAMETER -MASS(ES); ATTACHED TO ENDOMETRIUM OF DISTAL RIGHT HORN: SINGLE MASS, 27 X 7 X 7 MM, RED, DARK RED, GRAY AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT WITH UTERUS; REGIONAL LN (LUMBAR) NORMAL (CAP 22) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; LEFT: DRY RED MATERIAL -DISCHARGE-NASAL; BILATERAL: DRY RED MATERIAL UTERUS (UT) : -DILATATION,-MINIMAL -B-POLYP, ENDOMETRIAL, STROMAL,NEOPLASM PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT LN, LUMBAR/ILIAC (LM) : -HYPERPLASIA, LYMPHOPLASMACYTIC,-SLIGHT 2984 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1226 ANIMAL NUMBER: C92901 SEX: FEMALE DATE OF DEATH: 10/22/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 10/22/99 15:15 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 551 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: 268.1 GRAMS PROSECTOR: JARED FISHEL RECORDER: JARED FISHEL PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUm /s KULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 2985 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1227 ANIMAL NUMBER: C92902 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/27/99 STUDY DAY OF DEATH: 617 STUDY WEEK OF DEATH: 89 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/27/99 10:23 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -LIGHT FOCUS(I)/AREA(S); DIAPHRAGMATIC SURFACE OF LEFT LATERAL LOBE: SINGLE TAN AREA, 9 X 2 MM; COLLECTED ON ROUTINE SECTION -RED FOCUS(I)/AREA(S); DIAPHRAGMATIC SURFACE OF LEFT LATERAL LOBE: MULTIPLE DARK RED AREAS, UP TO 8 MM IN DIAMETER; SOME ARE SUNKEN WITH A STELLATE PATTERN; COLLECTED ON ROUTINE SECTION HISTOPATHOLOGY LIVER (LI) : -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -NECROSIS, COAGULATIVE,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SLIGHT -MINERALIZATION, PELVIC,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MINIMAL PANCREAS (PA) : -M-CARCINOMA, ISLET CELL,-NEOPLASM PRESENT ADRENAL, CORTEX (AC) : -PIGMENT,-MINIMAL 2986 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1228 ANIMAL NUMBER: C92902 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/27/99 STUDY DAY OF DEATH: 617 STUDY WEEK OF DEATH: 89 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/27/99 10:23 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -LARGE; 6 X 5 X 4 MM; APPEARS SWOLLEN MAMMARY, FEMALE (MF) : -THICKENED; VENTRAL CERVICAL REGION: TAN AND FIRM (CAP 21) THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),SEVERE >NOTE:>SEE SLIDE #16 THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL JEJUNUM (JE) : >AUTOLYTIC CECUM (CE) : >AUTOLYTIC MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT -HYPERPLASIA, GLANDULAR,-MODERATE -B-ADENOMA,-NEOPLASM PRESENT VAGINA (VA) : -LUMEN, EXUDATE,-PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, PANCREAS,-PRESENT CAVITY, ABDOM (PC) : >NOT REQUIRED TO BE EXAMINED FOR ANIMAL >NOTE:>SEE PANCREAS FOR DIAGNOSIS 2987 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1229 ANIMAL NUMBER: C92902 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/27/99 STUDY DAY OF DEATH: 617 STUDY WEEK OF DEATH: 89 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/27/99 10:23 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY CAVITY, ABDOM (PC) : -MASS(ES); ATTACHED TO ADIPOSE TISSUE OF COLON AND ILEUM: SINGLE MASS, 30 X 20 X 15 MM, RED, DARK RED, TAN, GRAY, VASCULAR AND FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (MESENTERIC) NORMAL GENERAL COMMENT (GC) : -STAINS - SKIN/HAIRCOAT; ENTIRE VENTRAL SURFACE: YELLOW, RED AND MOIST 2988 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1230 ANIMAL NUMBER: C92902 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/27/99 STUDY DAY OF DEATH: 617 STUDY WEEK OF DEATH: 89 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/27/99 10:23 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), ILEUM (IL), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), HEMATO NEOPLASIA (HN) (FM), 2989 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1231 ANIMAL NUMBER: C92903 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 349.9 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 11:35 PROSECTOR: STEVE VAN ADESTINE RECORDER: KIMBERLY DURLAND POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) LF ADRENAL (AD0) RT ADRENAL (AD1) BRAIN (BR) LF KIDNEY (KD0) RT KIDNEY (KD1) LIVER (LI) LUNG (LU) LF OVARY (OV0) RT OVARY (OV1) SPLEEN (SP) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) UTERUS (UT) .0438 .0435 2.1518 1.2498 1.2691 11.8895 1.5288 .0645 .0745 .5819 .0084 .0099 .7114 .0125 % .0124 % .6150 % .3572 % .3627 % 3.3980 % .4369 % .0184 % .0213 % .1663 % .0024 % .0028 % .2033 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0204 .0202 1.0000 .5808 .5898 5.5254 .7105 .0300 .0346 .2704 .0039 .0046 .3306 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED ADRENAL, CORTEX (AC) : >NOTE:>EMBEDDING COMMENT: ONE ADRENAL PRESENT AT EMBEDDING ADRENAL, MEDULLA (MA) : >NOTE:>EMBEDDING COMMENT: ONE ADRENAL PRESENT AT EMBEDDING SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT PANCREAS (PA) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL ADRENAL, CORTEX (AC) : -UNILATERALLY EXAMINED,-PRESENT ADRENAL, MEDULLA (MA) : -UNILATERALLY EXAMINED,-PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2990 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1232 ANIMAL NUMBER: C92903 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 349.9 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 11:35 PROSECTOR: STEVE VAN ADESTINE RECORDER: KIMBERLY DURLAND POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), LN, MESENTERIC (MS), OVARY (OV) 2991 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1233 ANIMAL NUMBER: C92904 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/21/00 STUDY DAY OF DEATH: 702 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: 470.7 GRAMS DATE AND TIME OF NECROPSY: 03/21/00 14:04 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); INGUINAL REGION-RIGHT; WEEK 70; 40 X 35MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); VENTRAL-THORACIC-LEFT; WEEK 73; 30 X 35MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); AXILLARY REGION-LEFT; WEEK 69; 55 X 65MM; VERIFIED AT NECROPSY: OBSERVED >WART LIKE LESION(S); MOUTH; WEEK 89; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK 97; 30 X 20MM; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) ADRENAL, CORTEX (AC) : -LARGE; BILATERAL: EACH IS 6 X 5 X 4 MM -MOTTLED; BILATERAL: RED, BROWN AND TAN HISTOPATHOLOGY BRAIN (BR) : >UNREMARKABLE LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-SLIGHT -NECROSIS, COAGULATIVE,-SLIGHT KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : -HYPERTROPHY, CORTICAL CELL,-SLIGHT -DEGENERATION, CYSTIC / HEMORRHAGIC,MODERATE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MINIMAL 2992 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1234 ANIMAL NUMBER: C92904 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/21/00 STUDY DAY OF DEATH: 702 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: 470.7 GRAMS DATE AND TIME OF NECROPSY: 03/21/00 14:04 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 6 X 5 X 3 MM, RED, DARK RED, TAN AND SOFT SKIN (SK) : -RAISED FOCUS(I)/AREA(S); LEFT UPPER LIP: SINGLE RED, TAN, FIRM RAISED AREA, 4 X 2 X 2 MM (CAP 23) MAMMARY, FEMALE (MF) : -MASS(ES); LEFT INGUINAL REGION: SINGLE MASS, 45 X 40 X 22 MM, RED, TAN AND FIRM; SAME ON CUT SURFACE (CAP 24); REGIONAL LN (INGUINAL) NOT IDENTIFIED -MASS #2; RIGHT ABDOMINAL REGION: SINGLE MASS, 45 X 30 X 20 MM, RED, TAN, FIRM AND LOBULATED; SAME ON CUT SURFACE (CAP 25); REGIONAL LN (INGUINAL) NOT IDENTIFIED LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL HEART (HT) : -CARDIOMYOPATHY,-MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-SLIGHT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL SKIN (SK) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE -B-PAPILLOMA, SQUAMOUS CELL,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -B-FIBROADENOMA,- 3 NEOPLASMS PRESENT 2993 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1235 ANIMAL NUMBER: C92904 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/21/00 STUDY DAY OF DEATH: 702 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: 470.7 GRAMS DATE AND TIME OF NECROPSY: 03/21/00 14:04 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS #3; LEFT AXILLARY REGION EXTENDS LEFT INGUINAL REGION: MULTIPLE MASSES, UP TO 65 X 55 X 40 MM (130.6 GRAMS), RED, DARK RED, TAN, FIRM AND LOBULATED; SAME ON CUT SURFACE (CAP 26,27); REGIONAL LN (SEE AXILLARY) UTERUS (UT) : -CYST(S); BILATERAL, WITHIN WALLS: MULTIPLE CLEAR FLUID FILLED CYSTS, UP TO 1 MM IN DIAMETER LN, AXILLARY (AX) : -DIFFUSELY DARK; BILATERAL: BROWN (CAP 22) LN, MANDIBULAR (MN) : -LARGE; LEFT: 12 X 6 X 4 MM 4 X 3 X 2 MM) (CAP 21) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN (RIGHT IS UTERUS (UT) : -HYPERPLASIA, CYSTIC, ENDOMETRIAL,MODERATE VAGINA (VA) : -HYPERPLASIA, MUCINOUS,-SLIGHT CAUSE OF DEATH (CD) : -NEOPLASM, MAMMARY GLAND,-PRESENT LN, AXILLARY (AX) : -HEMORRHAGE,-PRESENT -HYPERPLASIA, LYMPHOPLASMACYTIC,-SLIGHT -INFILTRATE, MACROPHAGE, PIGMENTED,MODERATE LN, MANDIBULAR (MN) : -HYPERPLASIA, LYMPHOPLASMACYTIC,-SEVERE 2994 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1236 ANIMAL NUMBER: C92904 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/21/00 STUDY DAY OF DEATH: 702 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: 470.7 GRAMS DATE AND TIME OF NECROPSY: 03/21/00 14:04 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), CERVIX (CV), HEMATO NEOPLASIA (HN) (ST), 2995 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1237 ANIMAL NUMBER: C92905 SEX: FEMALE DATE OF DEATH: 07/21/98 STUDY DAY OF DATE AND TIME OF NECROPSY: 07/21/98 13:02 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 250.0 GRAMS PROSECTOR: JOHN S. HALFORD RECORDER: MATTHEW AXTMAN PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: MATTHEW AXTMAN ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) LF ADRENAL (AD0) RT ADRENAL (AD1) BRAIN (BR) LF KIDNEY (KD0) RT KIDNEY (KD1) LIVER (LI) LUNG (LU) LF OVARY (OV0) RT OVARY (OV1) SPLEEN (SP) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) UTERUS (UT) .0460 .0463 2.0984 1.2015 1.2025 7.9055 1.4232 .1031 .0740 .5258 .0066 .0153 1.2028 .0184 % .0185 % .8394 % .4806 % .4810 % 3.1622 % .5693 % .0412 % .0296 % .2103 % .0026 % .0061 % .4811 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0219 .0221 1.0000 .5726 .5731 3.7674 .6782 .0491 .0353 .2506 .0031 .0073 .5732 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED UTERUS (UT) : -LUMEN FILLED WITH FLUID; BILATERAL: CLEAR FLUID GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT UTERUS (UT) : >NOT REQUIRED TO BE EXAMINED FOR ANIMAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2996 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1238 ANIMAL NUMBER: C92905 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 250.0 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 13:02 PROSECTOR: JOHN S. HALFORD RECORDER: MATTHEW AXTMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: MATTHEW AXTMAN THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV) 2997 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1239 ANIMAL NUMBER: C92906 SEX: FEMALE DATE OF DEATH: 04/19/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/19/00 7:41 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 343.6 GRAMS PROSECTOR: GREGORY CODDINGTON RECORDER: TERRI L. WILLIAMS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); AXILLARY REGION-RIGHT; WEEK 95; 40 X 30MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); AXILLARY REGION-LEFT; WEEK 95; 20 X 20MM; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) ADRENAL, CORTEX (AC) : -LARGE; RIGHT: 8 MM IN DIAMETER (LEFT IS 4 MM IN DIAMETER) HISTOPATHOLOGY EYE (EY) : -DYSTROPHY, CORNEAL IN EARLY STAGES, CONSISTS PRIMARILY OF FOCAL MINERALIZATION,-MODERATE BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-SLIGHT -HYPERTROPHY, HEPATOCELLULAR, PERIPORTAL,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, PELVIC,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT -HYPERTROPHY, CORTICAL CELL,-SLIGHT -PIGMENT,-SLIGHT ADRENAL, MEDULLA (MA) : -B-PHEOCHROMOCYTOMA,-NEOPLASM PRESENT OVARY (OV) : -HYPERPLASIA, GRANULOSA CELL,-SEVERE 2998 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1240 ANIMAL NUMBER: C92906 SEX: FEMALE DATE OF DEATH: 04/19/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/19/00 7:41 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 343.6 GRAMS PROSECTOR: GREGORY CODDINGTON RECORDER: TERRI L. WILLIAMS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 9 X 7 X 7 MM, RED, DARK RED, TAN AND SOFT MAMMARY, FEMALE (MF) : -MASS(ES); LEFT AXILLARY REGION: SINGLE MASS, 30 X 25 X 18 MM, TAN AND SOFT; ON SECTION EXUDES A TAN AND CREAMY SEMIFLUID; (CAP 22); REGIONAL LN (LEFT AXILLARY) NORMAL (CAP 23) -MASS #2; RIGHT AXILLARY REGION: SINGLE MASS, 47 X 35 X 25 MM, RED, DARK RED, TAN AND FIRM; SAME ON SURFACE; (CAP 24); REGIONAL LN (SEE AXILLARY) LN, AXILLARY (AX) : -DIFFUSELY RED; RIGHT: (CAP 23) THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),SLIGHT THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT -B-FIBROADENOMA,- 2 NEOPLASMS PRESENT -HYPERPLASIA, GLANDULAR,-SLIGHT UTERUS (UT) : -HYPERPLASIA, CYSTIC, ENDOMETRIAL,MINIMAL VAGINA (VA) : -LUMEN, EXUDATE,-PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, AXILLARY (AX) : -HEMORRHAGE,-PRESENT 2999 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1241 ANIMAL NUMBER: C92906 SEX: FEMALE DATE OF DEATH: 04/19/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/19/00 7:41 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 343.6 GRAMS PROSECTOR: GREGORY CODDINGTON RECORDER: TERRI L. WILLIAMS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -MALOCCLUSION; RIGHT UPPER INCISOR -DISCHARGE-OCULAR; BILATERAL: DARK RED AND DRY LN, AXILLARY (AX) : -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL 3000 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1242 ANIMAL NUMBER: C92906 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 343.6 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 7:41 PROSECTOR: GREGORY CODDINGTON RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), HEMATO NEOPLASIA (HN) 3001 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1243 ANIMAL NUMBER: C92907 SEX: FEMALE DATE OF DEATH: 04/21/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/21/00 8:17 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 468.7 GRAMS PROSECTOR: JOHN S. HALFORD RECORDER: LAURIE J. SCHULLER PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: NOT OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE RED AND DARK RED AREAS, UP TO 4 X 3 MM; COLLECTED ON ROUTINE SECTIONS ADRENAL, CORTEX (AC) : -LARGE; LEFT: 6 MM IN DIAMETER (RIGHT IS 4 MM IN DIAMETER) HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, PERIPORTAL,-SLIGHT -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, PELVIC,-SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SLIGHT ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATE -HYPERTROPHY, CORTICAL CELL,-MINIMAL -PIGMENT,-SLIGHT ADRENAL, MEDULLA (MA) : -UNILATERALLY EXAMINED,-PRESENT THYMUS (TH) : -HYPERPLASIA, LYMPHORETICULAR,MODERATELY SEVERE 3002 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1244 ANIMAL NUMBER: C92907 SEX: FEMALE DATE OF DEATH: 04/21/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/21/00 8:17 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 468.7 GRAMS PROSECTOR: JOHN S. HALFORD RECORDER: LAURIE J. SCHULLER PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY THYMUS (TH) : -MASS(ES); INVOLVING ENTIRE ORGAN: SINGLE TAN AND LOBULATED MASS, 15 X 13 X 6 MM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (MEDIASTINAL) NORMAL (CAP 25) PITUITARY (PI) : -LARGE; 5 X 4 X 3 MM, APPEARS SWOLLEN STOMACH, GL (ST) : -LIGHT FOCUS(I)/AREA(S); MUCOSA: MULTIPLE GRAY AREAS, UP TO 2 X 1 MM UTERUS (UT) : -CYST(S); RIGHT HORN WITHIN WALL: MULTIPLE CLEAR FLUID FILLED CYSTS; UP TO 6 MM IN DIAMETER LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL PITUITARY (PI) : -HYPERPLASIA,-SLIGHT HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-SLIGHT STOMACH, GL (ST) : >UNREMARKABLE ILEUM (IL) : -HYPERPLASIA, GALT (GUT ASSOCIATED LYMPHOID TISSUE),-MODERATE SKIN (SK) : -B-ADENOMA, BASAL CELL/SQUAMOUS/SEBACEOUS,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT UTERUS (UT) : -DILATATION,-MODERATELY SEVERE 3003 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1245 ANIMAL NUMBER: C92907 SEX: FEMALE DATE OF DEATH: 04/21/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/21/00 8:17 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 468.7 GRAMS PROSECTOR: JOHN S. HALFORD RECORDER: LAURIE J. SCHULLER PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL: DARK RED (CAP 22) CLITORAL GLAND (CL) : -MASS(ES); INVOLVING ENTIRE ORGAN: SINGLE MASS, 21 X 18 X 13 MM, RED, DARK RED, BLACK, AND SOFT; SAME ON CUT SURFACE (CAP 23); REGIONAL LN (INGUINAL) NORMAL (CAP 24) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MEDIASTINAL (ML) : -HYPERPLASIA, LYMPHOPLASMACYTIC,MODERATELY SEVERE LN, INGUINAL (IN) : >TISSUE MISSING LN, MANDIBULAR (MN) : -HYPERPLASIA, LYMPHOPLASMACYTIC,MODERATE -HEMORRHAGE,-PRESENT CLITORAL GLAND (CL) : >NOT REQUIRED TO BE EXAMINED FOR ANIMAL >NOTE:>SEE SKIN FOR DIAGNOSIS 3004 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1246 ANIMAL NUMBER: C92907 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 468.7 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 8:17 PROSECTOR: JOHN S. HALFORD RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 3005 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1247 ANIMAL NUMBER: C92908 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 646 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: 349.3 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 7:19 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); VENTRAL-CERVICAL; WEEK 78; 20 X 20MM; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; RECUMBENT; LATERAL; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; ATAXIC; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) HISTOPATHOLOGY BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, PELVIC,-MINIMAL -MINERALIZATION, TUBULAR,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-SLIGHT -INFILTRATION, FAT, INTERSTITIAL,SLIGHT ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL -PIGMENT,-MINIMAL 3006 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1248 ANIMAL NUMBER: C92908 SEX: FEMALE DATE OF DEATH: 01/25/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 01/25/00 7:19 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 646 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: 349.3 GRAMS PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 10 X 10 X 8 MM, RED, DARK RED AND SOFT MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT VENTRAL CERVICAL REGION: SINGLE MASS, 26 X 21 X 17 MM, RED, DARK RED, GREY, TAN AND SOFT; ON SECTION EXUDES A TAN, CREAMY SEMIFLUID MATERIAL; COLLECTED; REGIONAL LN (MANDIBULAR) NORMAL (CAP 21) -THICKENED; ALL REGIONS, DIFFUSE: TAN; COLLECTED ON ROUTINE SECTION ADRENAL, MEDULLA (MA) : -UNILATERALLY EXAMINED,-PRESENT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),SLIGHT HEART (HT) : -METAPLASIA, OSSEOUS / CARTILAGINOUS,MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-SLIGHT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL MAMMARY, FEMALE (MF) : -M-CARCINOMA,-NEOPLASM PRESENT -CYST / GALACTOCELE,-PRESENT UTERUS (UT) : -DILATATION,-SLIGHT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 3007 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1249 ANIMAL NUMBER: C92908 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 646 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: 349.3 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 7:19 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) -BLOOD FILM TAKEN -DISCHARGE-OCULAR; MATERIAL -ANIMAL THIN : RIGHT: DRY, RED LN, MANDIBULAR (MN) : -HYPERPLASIA, LYMPHOPLASMACYTIC,-SLIGHT 3008 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1250 ANIMAL NUMBER: C92908 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 646 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: 349.3 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 7:19 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 3009 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1251 ANIMAL NUMBER: C92909 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 468.5 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 10:06 PROSECTOR: TROY ELLENBECKER RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); VENTRAL-ABDOMINAL-RIGHT; WEEK 98; 80 X 45MM; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY KIDNEY (KD) : -LARGE PELVIS(ES); BILATERAL -DIFFUSELY LIGHT; BILATERAL: TAN HISTOPATHOLOGY LIVER (LI) : -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-SLIGHT -HYPERPLASIA, BILE DUCT,-SLIGHT -FIBROSIS, PERIBILIARY,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SLIGHT -DILATATION, PELVIC,-SLIGHT -MINERALIZATION, PELVIC,-MINIMAL -VACUOLATION, TUBULAR EPITHELIUM,MODERATE SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATELY SEVERE PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-MODERATE -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL ADRENAL, CORTEX (AC) : -HYPERTROPHY, CORTICAL CELL,-MODERATE -DEGENERATION, CYSTIC / HEMORRHAGIC,MODERATE -PIGMENT,-MINIMAL 3010 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1252 ANIMAL NUMBER: C92909 SEX: FEMALE DATE OF DEATH: 04/20/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/20/00 10:06 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 468.5 GRAMS PROSECTOR: TROY ELLENBECKER RECORDER: JERALD W. JOHNSON PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY SKIN (SK) : >NOTE:>COLLECTED FROM RIGHT INGUINAL REGION DUE TO MF MASS MAMMARY, FEMALE (MF) : -MASS(ES); MID INGUINAL REGION EXTENDING TO PERIANAL REGION: SINGLE MASS, 120 X 50 X 35 MM (127.0 GRAMS) RED, DARK RED, TAN, LOBULATED AND SOFT; SAME ON CUT SURFACE; SOME SECTIONS EXUDE A TAN SEMIFLUID MATERIAL; COLLECTED INTACT; REGIONAL LN (INGUINAL) NOT IDENTIFIED >NOTE:>COLLECTED FROM RIGHT INGUINAL REGION DUE TO MF MASS LN, MESENTERIC (MS) : -HEMORRHAGE,-PRESENT -INFILTRATE, MACROPHAGE, PIGMENTED,SLIGHT THYMUS (TH) : >SECTION EXAMINED; TISSUE NOT PRESENT LUNG (LU) : -N-CARCINOMA,-NEOPLASM PRESENT PITUITARY (PI) : -HYPERPLASIA,-MINIMAL MAMMARY, FEMALE (MF) : -M-CARCINOMA,-NEOPLASM PRESENT -HYPERPLASIA, GLANDULAR,-MODERATE UTERUS (UT) : -HYPERPLASIA, MINIMAL VAGINA (VA) : -HYPERPLASIA, CYSTIC, ENDOMETRIAL,MUCINOUS,-MODERATE 3011 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1253 ANIMAL NUMBER: C92909 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 468.5 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 10:06 PROSECTOR: TROY ELLENBECKER RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY LN, LUMBAR/ILIAC (LM) : -MOTTLED; BILATERAL: RED AND TAN (CAP 23) URETER (UE) : -LARGE; BILATERAL: EACH IS UP TO 1 MM IN DIAMETER (CAP 24) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -URINARY BLADDER-LARGE LUMEN; 40 X 25 X 20 MM VAGINA (VA) : -I-CARCINOMA,-FROM MAMMARY, FEMALE CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, LUMBAR/ILIAC (LM) : -N-CARCINOMA,-FROM MAMMARY, FEMALE URETER (UE) : >TISSUE MISSING 3012 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1254 ANIMAL NUMBER: C92909 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 468.5 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 10:06 PROSECTOR: TROY ELLENBECKER RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), ADRENAL, MEDULLA (MA), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), HEMATO NEOPLASIA (HN) (SE), 3013 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1255 ANIMAL NUMBER: C92910 SEX: FEMALE DATE OF DEATH: 04/20/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/20/99 10:35 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 387.5 GRAMS PROSECTOR: NANCY DIEDRICH RECORDER: TONY HANFELD PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) LF ADRENAL (AD0) RT ADRENAL (AD1) BRAIN (BR) LF KIDNEY (KD0) RT KIDNEY (KD1) LIVER (LI) LUNG (LU) LF OVARY (OV0) RT OVARY (OV1) SPLEEN (SP) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) UTERUS (UT) .0472 .0441 2.1542 1.3866 1.3904 10.9820 1.7913 .0259 .0379 .5683 .0124 .0103 1.1855 .0122 % .0114 % .5559 % .3578 % .3588 % 2.8341 % .4623 % .0067 % .0098 % .1467 % .0032 % .0027 % .3059 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0219 .0205 1.0000 .6437 .6454 5.0979 .8315 .0120 .0176 .2638 .0058 .0048 .5503 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MINIMAL ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC / HEMORRHAGIC MODERATE LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL 3014 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1256 ANIMAL NUMBER: C92910 SEX: FEMALE DATE OF DEATH: 04/20/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/20/99 10:35 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 387.5 GRAMS PROSECTOR: NANCY DIEDRICH RECORDER: TONY HANFELD PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -DARK FOCUS(I)/AREA(S); MID ASPECT: SINGLE DARK BROWN AREA, 5 X 2 MM LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL: DARK RED (CAP 20) GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN -MALOCCLUSION; BILATERAL UPPER INCISORS LUNG (LU) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL HEART (HT) : -CARDIOMYOPATHY,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT -ANGIECTASIS,-PRESENT MAMMARY, FEMALE (MF) : -HYPERPLASIA, GLANDULAR,-MINIMAL -SECRETORY MATERIAL,-PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MANDIBULAR (MN) : -HYPERPLASIA, LYMPHOPLASMACYTIC,MODERATELY SEVERE -HEMORRHAGE,-PRESENT 3015 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1257 ANIMAL NUMBER: C92910 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 387.5 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 10:35 PROSECTOR: NANCY DIEDRICH RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LIVER (LI), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 3016 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1258 ANIMAL NUMBER: C92911 SEX: FEMALE DATE OF DEATH: 04/20/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/20/00 8:05 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 357.5 GRAMS PROSECTOR: CHANESTA ACKER RECORDER: TONE EVERTS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: NOT OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -RED FOCUS(I)/AREA(S); DIAPHRAGMATIC SURFACE OF LEFT LATERAL LOBE: SINGLE DARK RED AREA, 9 X 6 MM; COLLECTED ON ROUTINE SECTION HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-SLIGHT -HYPERPLASIA, BILE DUCT,-MINIMAL -ANGIECTASIS,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, PELVIC,-MINIMAL -MINERALIZATION, TUBULAR,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SLIGHT ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATE -HYPERTROPHY, CORTICAL CELL,-SLIGHT -DEGENERATION, CYSTIC / HEMORRHAGIC,SLIGHT -HYPERPLASIA, CORTICAL,-MINIMAL -PIGMENT,-MINIMAL 3017 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1259 ANIMAL NUMBER: C92911 SEX: FEMALE DATE OF DEATH: 04/20/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/20/00 8:05 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 357.5 GRAMS PROSECTOR: CHANESTA ACKER RECORDER: TONE EVERTS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 6 X 5 X 3 MM, DARK RED, TAN, AND SOFT MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT INGUINAL REGION: SINGLE MASS, 25 X 17 X 10 MM, DARK RED, RED, TAN, FIRM, AND GELATINOUS; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED -THICKENED; ALL REGIONS DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION -CYST(S); ALL REGIONS DIFFUSE: MULTIPLE WHITE CREAMY FLUID FILLED CYSTS; COLLECTED ON ROUTINE SECTION GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN HEART (HT) : -CARDIOMYOPATHY,-MINIMAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -M-CARCINOMA,-NEOPLASM PRESENT -CYST / GALACTOCELE,-PRESENT -HYPERPLASIA, GLANDULAR,-SLIGHT UTERUS (UT) : -HYPERPLASIA, CYSTIC, ENDOMETRIAL,MINIMAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 3018 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1260 ANIMAL NUMBER: C92911 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 357.5 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 8:05 PROSECTOR: CHANESTA ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 3019 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1261 ANIMAL NUMBER: C92912 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 704.5 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 8:30 PROSECTOR: JAMES T. CALLEN RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK 80; 90 X 100MM; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; VENTRAL ABDOMINAL; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) OVARY (OV) : -CYST(S); RIGHT: SINGLE CLEAR FLUID FILLED CYST, 5 MM IN DIAMETER HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, PERIPORTAL,-MODERATE KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MINIMAL -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-MINIMAL -HYPERTROPHY, CORTICAL CELL,-MODERATE -DEGENERATION, CYSTIC / HEMORRHAGIC,MODERATE -PIGMENT,-SLIGHT OVARY (OV) : -CYST,-PRESENT -HYPERPLASIA, GRANULOSA CELL,-MODERATE 3020 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1262 ANIMAL NUMBER: C92912 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 704.5 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 8:30 PROSECTOR: JAMES T. CALLEN RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 7 X 7 X 5 MM, DARK RED AND FIRM MAMMARY, FEMALE (MF) : -MASS(ES); EXTENDS FROM RIGHT INGUINAL TO LEFT INGUINAL REGION: SINGLE MASS, 115 X 75 X 30 MM (190.6 GRAMS), TAN, RED, LOBULATED, VASCULAR AND FIRM; SAME ON CUT SURFACE; PORTION COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED; OVERLYING SKIN HAS A TAN DRY CRUSTED AREA, 30 X 20 MM; COLLECTED INTACT WITH MASS VAGINA (VA) : -SEMIFLUID MATERIAL; WITHIN LUMEN: CONTAINS A LIGHT GREEN SEMIFLUID MATERIAL MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,-MODERATE MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,-SLIGHT THYMUS (TH) : >TISSUE MISSING LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT -B-FIBROADENOMA,-NEOPLASM PRESENT -HYPERPLASIA, GLANDULAR,-MINIMAL VAGINA (VA) : -HYPERPLASIA, MUCINOUS,-MINIMAL 3021 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1263 ANIMAL NUMBER: C92912 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 704.5 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 8:30 PROSECTOR: JAMES T. CALLEN RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUm /s KULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), HEMATO NEOPLASIA (HN) 3022 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1264 ANIMAL NUMBER: C92913 SEX: FEMALE DATE OF DEATH: 04/19/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/19/00 7:38 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 406.9 GRAMS PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); VENTRAL-ABDOMINAL; WEEK 91; 50 X 45MM; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; VENTRAL ABDOMINAL; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY ADRENAL, CORTEX (AC) : 6-LARGE; RIGHT: 7 X X 4 MM (LEFT IS 4 X 4 X 3 MM) HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MODERATE -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-SLIGHT -HYPERPLASIA, BILE DUCT,-SLIGHT -FIBROSIS, PERIBILIARY,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, PELVIC,-MINIMAL -HYPERPLASIA, TRANSITIONAL CELL,MINIMAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATELY SEVERE ADRENAL, CORTEX (AC) : -HEMATOPOIESIS, EXTRAMEDULLARY,-SLIGHT -HYPERTROPHY, CORTICAL CELL,-SLIGHT -DEGENERATION, CYSTIC / HEMORRHAGIC,MODERATE -PIGMENT,-SLIGHT 3023 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1265 ANIMAL NUMBER: C92913 SEX: FEMALE DATE OF DEATH: 04/19/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/19/00 7:38 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 406.9 GRAMS PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -LARGE; 7 X 5 X 4 MM; APPEARS SWOLLEN SKIN (SK) : >NOTE:>ROUTINE SECTION COLLECTED FROM RIGHT INGUINAL REGION DUE TO MASS MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,-MODERATE MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,-MODERATE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),SLIGHT LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : >UNREMARKABLE HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL MAMMARY, FEMALE (MF) : -M-CARCINOMA,-NEOPLASM PRESENT -CYST / GALACTOCELE,-PRESENT -HYPERPLASIA, GLANDULAR,-SLIGHT 3024 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1266 ANIMAL NUMBER: C92913 SEX: FEMALE DATE OF DEATH: 04/19/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/19/00 7:38 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 406.9 GRAMS PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS(ES); LEFT INGUINAL REGION: SINGLE MASS, 55 X 35 X 30 MM, RED, DARK RED, TAN AND FIRM; SAME ON CUT SURFACE; COLLECTED; OVERLYING SKIN HAS A SINGLE DARK RED AND MOIST ULCERATED AREA, 45 X 20 MM; COLLECTED INTACT WITH MASS; REGIONAL LN (INGUINAL) NOT IDENTIFIED >NOTE:>ROUTINE SECTION COLLECTED FROM RIGHT INGUINAL REGION DUE TO MASS GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN UTERUS (UT) : -HYPERPLASIA, CYSTIC, ENDOMETRIAL,MODERATE VAGINA (VA) : -HYPERPLASIA, MUCINOUS,-SEVERE CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 3025 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1267 ANIMAL NUMBER: C92913 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 406.9 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 7:38 PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), HEMATO NEOPLASIA (HN) 3026 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1268 ANIMAL NUMBER: C92914 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 181.3 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 10:53 PROSECTOR: LYNN HORNER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN STATUS LF ADRENAL (AD0) RT ADRENAL (AD1) BRAIN (BR) LF KIDNEY (KD0) RT KIDNEY (KD1) LIVER (LI) LUNG (LU) LF OVARY (OV0) RT OVARY (OV1) SPLEEN (SP) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) UTERUS (UT) ------------------------------------ 7.2302 -------------------------------------------------- ------------------------------------ 3.9878 % -------------------------------------------------- -------------------------------------------------------------------------------------------- NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN WEIGHT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS 3027 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1269 ANIMAL NUMBER: C92914 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 181.3 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 10:53 PROSECTOR: LYNN HORNER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) 3028 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1270 ANIMAL NUMBER: C92915 SEX: FEMALE DATE OF DEATH: 02/18/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 02/18/00 8:20 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 670 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: 292.7 GRAMS PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; SORE/SCAB; PAWS-HIND; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; NO FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >EYES; RED DISCHARGE; EYES; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) HISTOPATHOLOGY BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -NECROSIS, INDIVIDUAL HEPATOCYTE,MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-MODERATE -HYPERPLASIA, BILE DUCT,-MINIMAL -FIBROSIS, PERIBILIARY,-MINIMAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-SEVERE -HYPERPLASIA, TRANSITIONAL CELL,MODERATE SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SLIGHT PANCREAS (PA) : -HYPERPLASIA, ISLET CELLS,-MINIMAL ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-MINIMAL -HYPERTROPHY, CORTICAL CELL,-SLIGHT -PIGMENT,-SLIGHT 3029 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1271 ANIMAL NUMBER: C92915 SEX: FEMALE DATE OF DEATH: 02/18/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 02/18/00 8:20 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 670 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: 292.7 GRAMS PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); SINGLE MASS, 11 X 10 X 6 MM, RED, DARK RED, TAN, GRAY, AND SOFT CERVIX (CV) : -FIRM; DIFFUSE -LARGE; 10 X 6 X 5 MM FOOT/FOOTPAD (FP) : -ABRASION(S)/ULCERATION(S); BILATERAL HINDFEET: SINGLE TAN AND MOIST AREA ON EACH, EACH IS 4 X 3 X 3 MM; BOTH COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),SLIGHT HEART (HT) : -CARDIOMYOPATHY,-MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -HYPERPLASIA, GLANDULAR,-SLIGHT CERVIX (CV) : >UNREMARKABLE VAGINA (VA) : -LUMEN, EXUDATE,-PRESENT -HYPERPLASIA, MUCINOUS,-SLIGHT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT FOOT/FOOTPAD (FP) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE -ULCERATION,-SLIGHT -ACANTHOSIS / HYPERKERATOSIS,-MODERATE 3030 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1272 ANIMAL NUMBER: C92915 SEX: FEMALE DATE OF DEATH: 02/18/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 02/18/00 8:20 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 670 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: 292.7 GRAMS PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -DISCHARGE-OCULAR; BILATERAL: DARK RED AND DRY THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), HEMATO NEOPLASIA (HN) 3031 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1273 ANIMAL NUMBER: C92916 SEX: FEMALE DATE OF DEATH: 01/28/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 01/28/00 11:15 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 649 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: 407.5 GRAMS PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); AXILLARY REGION-RIGHT; WEEK 46; 15 X 15MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); AXILLARY REGION-LEFT; WEEK 70; 10 X 10MM; VERIFIED AT NECROPSY: OBSERVED >WART LIKE LESION(S); MOUTH; WEEK 75; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); INGUINAL REGION-RIGHT; WEEK 75; 50 X 40MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); INGUINAL REGION-LEFT; WEEK 84; 20 X 20MM; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; PAW-HINDRIGHT; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; HEAD TILT; RIGHT; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) ADRENAL, CORTEX (AC) : -LARGE; LEFT: 4 X 3 X 3 MM (RIGHT IS 4 X 3 X 3 MM) HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SLIGHT -MINERALIZATION, PELVIC,-SLIGHT -MINERALIZATION, TUBULAR,-SLIGHT -HYPERPLASIA, TRANSITIONAL CELL,-SLIGHT PANCREAS (PA) : -ATROPHY, ACINAR,-MINIMAL ADRENAL, CORTEX (AC) : -HYPERTROPHY, CORTICAL CELL,-SLIGHT -DEGENERATION, CYSTIC / HEMORRHAGIC,SLIGHT -PIGMENT,-SLIGHT ADRENAL, MEDULLA (MA) : -B-PHEOCHROMOCYTOMA,-NEOPLASM PRESENT MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,-MODERATE MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,-MODERATE 3032 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1274 ANIMAL NUMBER: C92916 SEX: FEMALE DATE OF DEATH: 01/28/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 01/28/00 11:15 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 649 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: 407.5 GRAMS PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 10 X 5 X 4 MM, RED, DARK RED, GREY, TAN, LOBULATED AND SOFT; PORTIONS ADHERED TO BRAIN WITH MULTIPLE TAN FIBROUS ADHESIONS; COLLECTED INTACT SKIN (SK) : -CRUSTED AREA(S); ATTACHED TO LOWER RIGHT LIP: SINGLE DRY, BROWN CRUSTED AREA, 2 X 2 X 1 MM (CAP 21) MAMMARY, FEMALE (MF) : -MASS(ES); LEFT AXILLARY REGION: SINGLE TAN AND SOFT MASS, 16 X 11 X 7 MM; SAME ON CUT SURFACE (CAP 25); REGIONAL LN (AXILLARY) NORMAL (CAP 24) THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATE LUNG (LU) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL HEART (HT) : -CARDIOMYOPATHY,-MINIMAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT SKIN (SK) : -B-PAPILLOMA, SQUAMOUS CELL,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -M-CARCINOMA,- 3 NEOPLASMS PRESENT -CYST / GALACTOCELE,-PRESENT -B-FIBROADENOMA,-NEOPLASM PRESENT -HYPERPLASIA, GLANDULAR,-MODERATE >NOTE:>MASS #1 IS THE FIBROADENOMA 3033 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1275 ANIMAL NUMBER: C92916 SEX: FEMALE DATE OF DEATH: 01/28/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 01/28/00 11:15 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 649 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: 407.5 GRAMS PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS #2; RIGHT AXILLARY REGION: SINGLE MASS, 21 X 15 X 9 MM, RED, DARK RED, GREY, TAN, LOBULATED AND SOFT; ON SECTION EXUDES A DARK RED FLUID (CAP 26); REGIONAL LN (AXILLARY) NORMAL (CAP 24) -MASS #3; LEFT INGUINAL REGION: SINGLE MASS, 31 X 25 X 14 MM, RED, DARK RED, GREY, TAN, LOBULATED AND SOFT; SAME ON CUT SURFACE (CAP 27); REGIONAL LN (INGUINAL) LEFT NOT IDENTIFIED, RIGHT IS NORMAL (CAP 23) -MASS #4; RIGHT INGUINAL REGION: SINGLE MASS, 58 X 43 X 28 MM, RED, DARK RED, GREY, TAN, LOBULATED AND SOFT; SOME SECTIONS EXUDE A TAN, CREAMY SEMIFLUID; COLLECTED INTACT; REGIONAL LN (INGUINAL) NORMAL (CAP 23) CAUSE OF DEATH (CD) : -NEOPLASM, MAMMARY GLAND,-PRESENT LN, INGUINAL (IN) : >TISSUE MISSING >NOTE:>PIECE OF TISSUE THOUGHT TO BE LYMPH NODE IS ACTUALLY A PORTION OF THE MAMMARY TUMOR FOOT/FOOTPAD (FP) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE -B-FIBROMA,-NEOPLASM PRESENT 3034 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1276 ANIMAL NUMBER: C92916 SEX: FEMALE DATE OF DEATH: 01/28/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 01/28/00 11:15 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 649 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: 407.5 GRAMS PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY FOOT/FOOTPAD (FP) : -CRUSTED AREA(S); RIGHT HINDFOOT: SINGLE DRY, DARK RED CRUSTED AREA, 6 X 5 X 3 MM; SURROUNDING TISSUE APPEARS SWOLLEN (CAP 22) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DRY, RED MATERIAL -ANIMAL THIN 3035 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1277 ANIMAL NUMBER: C92916 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/28/00 STUDY DAY OF DEATH: 649 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: 407.5 GRAMS DATE AND TIME OF NECROPSY: 01/28/00 11:15 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN), LN, AXILLARY (AX) 3036 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1278 ANIMAL NUMBER: C92917 SEX: FEMALE DATE OF DEATH: 04/20/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/20/99 10:16 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 523.6 GRAMS PROSECTOR: JOHN S. HALFORD RECORDER: TONY HANFELD PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) LF ADRENAL (AD0) RT ADRENAL (AD1) BRAIN (BR) LF KIDNEY (KD0) RT KIDNEY (KD1) LIVER (LI) LUNG (LU) LF OVARY (OV0) RT OVARY (OV1) SPLEEN (SP) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) UTERUS (UT) .0691 .0419 2.1639 1.5698 1.5702 14.4167 1.8308 .0734 .1023 .6282 .0112 .0154 1.5030 .0132 % .0080 % .4133 % .2998 % .2999 % 2.7534 % .3497 % .0140 % .0195 % .1200 % .0021 % .0029 % .2871 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0319 .0194 1.0000 .7254 .7256 6.6624 .8461 .0339 .0473 .2903 .0052 .0071 .6946 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, PELVIC,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATELY SEVERE -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SLIGHT PANCREAS (PA) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL 3037 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1279 ANIMAL NUMBER: C92917 SEX: FEMALE DATE OF DEATH: 04/20/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/20/99 10:16 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 523.6 GRAMS PROSECTOR: JOHN S. HALFORD RECORDER: TONY HANFELD PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PANCREAS (PA) : -MASS(ES); AT CRANIAL END: SINGLE MASS, 19 X 15 X 8 MM, TAN LOBULATED AND FIRM; SAME ON CUT SURFACE (CAP 20); REGIONAL LN (PANCREATIC) NOT IDENTIFIED ADRENAL, CORTEX (AC) : -CYST(S); TRIMMING COMMENT: ADRENAL HAS A SINGLE CYST, 2 MM IN DIAMETER PITUITARY (PI) : -LARGE; 5 X 4 X 4 MM; APPEARS SWOLLEN MAMMARY, FEMALE (MF) : -CYST(S); ALL REGIONS, DIFFUSE: MULTIPLE WHITE AND CREAMY SEMIFLUID FILLED CYSTS, UP TO 6 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION UTERUS (UT) : -THICKENED WALL; BILATERAL, DIFFUSE LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL: DARK RED; COLLECTED GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN PANCREAS (PA) : -NECROSIS / INFLAMMATION, ADIPOSE TISSUE,-PRESENT ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC / HEMORRHAGIC,MODERATE LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT -ANGIECTASIS,-PRESENT MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT -HYPERPLASIA, GLANDULAR,-MODERATE -SECRETORY MATERIAL,-PRESENT UTERUS (UT) : >UNREMARKABLE CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MANDIBULAR (MN) : -HYPERPLASIA, LYMPHOPLASMACYTIC,MODERATE -HEMORRHAGE,-PRESENT 3038 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1280 ANIMAL NUMBER: C92917 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 523.6 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 10:16 PROSECTOR: JOHN S. HALFORD RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR),LIVER (LI), KIDNEY (KD), SPLEEN (SP), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR),ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE),BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 3039 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1281 ANIMAL NUMBER: C92918 SEX: FEMALE DATE OF DEATH: 09/09/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 09/09/99 14:24 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 508 STUDY WEEK OF DEATH: 73 TERMINAL BODY WEIGHT: 347.3 GRAMS PROSECTOR: TONY HANFELD RECORDER: TONY HANFELD PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >EYES; RED DISCHARGE; EYES; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); SINGLE MASS, 11 X 9 X 5 MM, DARK RED, TAN AND SOFT HISTOPATHOLOGY BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -CYST, BILIARY,-PRESENT KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : -PIGMENT,-MINIMAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT 3040 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1282 ANIMAL NUMBER: C92918 SEX: FEMALE DATE OF DEATH: 09/09/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 09/09/99 14:24 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 508 STUDY WEEK OF DEATH: 73 TERMINAL BODY WEIGHT: 347.3 GRAMS PROSECTOR: TONY HANFELD RECORDER: TONY HANFELD PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) -BLOOD FILM TAKEN -DISCHARGE-OCULAR; MATERIAL -ANIMAL THIN : BILATERAL: DRY RED CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 3041 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1283 ANIMAL NUMBER: C92918 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/09/99 STUDY DAY OF DEATH: 508 STUDY WEEK OF DEATH: 73 TERMINAL BODY WEIGHT: 347.3 GRAMS DATE AND TIME OF NECROPSY: 09/09/99 14:24 PROSECTOR: TONY HANFELD RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP),JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM(DP), CAVITY, THORACIC (TA),LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG),STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) (CE), 3042 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1284 ANIMAL NUMBER: C92919 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 234.6 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 9:13 PROSECTOR: LYNN HORNER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN STATUS LF ADRENAL (AD0) RT ADRENAL (AD1) BRAIN (BR) LF KIDNEY (KD0) RT KIDNEY (KD1) LIVER (LI) LUNG (LU) LF OVARY (OV0) RT OVARY (OV1) SPLEEN (SP) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) UTERUS (UT) ------------------------------------ 9.0816 -------------------------------------------------- ------------------------------------ 3.8714 % -------------------------------------------------- -------------------------------------------------------------------------------------------- NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN WEIGHT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS 3043 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1285 ANIMAL NUMBER: C92919 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 234.6 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 9:13 PROSECTOR: LYNN HORNER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) 3044 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1286 ANIMAL NUMBER: C92920 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/30/99 STUDY DAY OF DEATH: 498 STUDY WEEK OF DEATH: 72 TERMINAL BODY WEIGHT: 299.2 GRAMS DATE AND TIME OF NECROPSY: 08/30/99 13:55 PROSECTOR: DENNIS HOFFMAN RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >EXCRETION; NO FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; HUNCHED; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -DIFFUSELY LIGHT; ALL LOBES: TAN -RED FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE RED AREAS, UP TO 3 X 4 MM; COLLECTED ON ROUTINE SECTIONS KIDNEY (KD) : -CYST(S); RIGHT, CORTICAL SURFACE: SINGLE CLEAR FLUID FILLED CYST, 2 MM IN DIAMETER PITUITARY (PI) : -MASS(ES); SINGLE MASS, 7 X 9 X 9 MM, SOFT, RED, DARK RED, TAN AND BROWN HISTOPATHOLOGY BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-MODERATELY SEVERE KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MINIMAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT 3045 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1287 ANIMAL NUMBER: C92920 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/30/99 STUDY DAY OF DEATH: 498 STUDY WEEK OF DEATH: 72 TERMINAL BODY WEIGHT: 299.2 GRAMS DATE AND TIME OF NECROPSY: 08/30/99 13:55 PROSECTOR: DENNIS HOFFMAN RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA: MULTIPLE TAN ERODED AREAS, UP TO 1 X 3 MM MAMMARY, FEMALE (MF) : -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION -CYST(S); ALL REGION, DIFFUSE: MULTIPLE WHITE FLUID FILLED CYSTS, UP TO 4 MM IN DIAMETER (CAP 20) VAGINA (VA) : -SEMIFLUID MATERIAL; WITHIN LUMEN: LIGHT GREEN MUCOID MATERIAL GENERAL COMMENT (GC) : -BLOOD FILM TAKEN STOMACH, NONGL (SU) : -ACANTHOSIS / HYPERKERATOSIS,-MODERATE -ULCERATION,-MINIMAL -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE VAGINA (VA) : -LUMEN, EXUDATE,-PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 3046 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1288 ANIMAL NUMBER: C92920 SEX: FEMALE DATE OF DEATH: 08/30/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 08/30/99 13:55 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 498 STUDY WEEK OF DEATH: 72 TERMINAL BODY WEIGHT: 299.2 GRAMS PROSECTOR: DENNIS HOFFMAN RECORDER: JARED FISHEL PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), HEMATO NEOPLASIA (HN) (HG), 3047 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1289 ANIMAL NUMBER: C92921 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/17/99 STUDY DAY OF DEATH: 393 STUDY WEEK OF DEATH: 57 TERMINAL BODY WEIGHT: 299.9 GRAMS DATE AND TIME OF NECROPSY: 05/17/99 14:22 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); AXILLARY REGION-LEFT; WEEK 48; 15 X 15MM; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; ROUGH HAIR COAT; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; RED HAIR COAT; HEADENTIRE; VERIFIED AT NECROPSY: NOT OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >EYES; RED DISCHARGE; EYES; VERIFIED AT NECROPSY: NOT OBSERVED >SKIN & PELAGE; SORE/SCAB; AXILLARY REGION-LEFT; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >RESPIRATION; LABORED; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 15 X 8 X 8 MM, DARK RED, GREY AND SOFT HISTOPATHOLOGY BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATELY SEVERE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),SLIGHT THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT SKIN (SK) : >UNREMARKABLE >NOTE:>SEE MAMMARY GLAND FOR DIAGNOSIS 3048 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1290 ANIMAL NUMBER: C92921 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/17/99 STUDY DAY OF DEATH: 393 STUDY WEEK OF DEATH: 57 TERMINAL BODY WEIGHT: 299.9 GRAMS DATE AND TIME OF NECROPSY: 05/17/99 14:22 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY SKIN (SK) : -MASS(ES); LEFT AXILLARY REGION: SINGLE MASS, 30 X 20 X 18 MM, RED, TAN AND SOFT; SAME ON CUT SURFACE; COLLECTED INTACT; OVERLYING SKIN IS BLACK AND CRUSTED; COLLECTED WITH MASS; REGIONAL LN (AXILLARY) NORMAL (CAP 20) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN MAMMARY, FEMALE (MF) : -M-CARCINOMA,-NEOPLASM PRESENT -CYST / GALACTOCELE,-PRESENT -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL -HYPERPLASIA, GLANDULAR,-MINIMAL CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT LN, AXILLARY (AX) : -HYPERPLASIA, LYMPHOPLASMACYTIC,MODERATE 3049 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1291 ANIMAL NUMBER: C92921 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/17/99 STUDY DAY OF DEATH: 393 STUDY WEEK OF DEATH: 57 TERMINAL BODY WEIGHT: 299.9 GRAMS DATE AND TIME OF NECROPSY: 05/17/99 14:22 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 3050 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1292 ANIMAL NUMBER: C92922 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 454.9 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 10:53 PROSECTOR: KEVIN BILLINGS RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) LF ADRENAL (AD0) RT ADRENAL (AD1) BRAIN (BR) LF KIDNEY (KD0) RT KIDNEY (KD1) LIVER (LI) LUNG (LU) LF OVARY (OV0) RT OVARY (OV1) SPLEEN (SP) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) UTERUS (UT) .0303 .0315 2.2274 1.3199 1.4231 11.3904 1.6016 .0896 .0890 .6828 .0105 .0145 .7457 .0067 % .0069 % .4896 % .2902 % .3128 % 2.5039 % .3521 % .0197 % .0196 % .1501 % .0023 % .0032 % .1639 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0136 .0141 1.0000 .5926 .6389 5.1138 .7190 .0402 .0400 .3065 .0047 .0065 .3348 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -NECROSIS, INDIVIDUAL HEPATOCYTE,MINIMAL -VACUOLATION, HEPATOCELLULAR,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC / HEMORRHAGIC,SLIGHT 3051 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1293 ANIMAL NUMBER: C92922 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 454.9 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 10:53 PROSECTOR: KEVIN BILLINGS RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN LUNG (LU) : -HEMORRHAGE,-PRESENT -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT -HYPERPLASIA, GLANDULAR,-SLIGHT -SECRETORY MATERIAL,-PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 3052 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1294 ANIMAL NUMBER: C92922 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 454.9 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 10:53 PROSECTOR: KEVIN BILLINGS RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 3053 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1295 ANIMAL NUMBER: C92923 SEX: FEMALE DATE OF DEATH: 04/20/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/20/99 9:35 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 374.6 GRAMS PROSECTOR: TODD ACKER RECORDER: TONY HANFELD PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) LF ADRENAL (AD0) RT ADRENAL (AD1) BRAIN (BR) LF KIDNEY (KD0) RT KIDNEY (KD1) LIVER (LI) LUNG (LU) LF OVARY (OV0) RT OVARY (OV1) SPLEEN (SP) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) UTERUS (UT) .0305 .0273 2.1318 1.0985 1.1508 9.0597 1.3671 .0361 .0412 .5812 .0102 .0114 1.1680 .0081 % .0073 % .5691 % .2932 % .3072 % 2.4185 % .3649 % .0096 % .0110 % .1552 % .0027 % .0030 % .3118 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0143 .0128 1.0000 .5153 .5398 4.2498 .6413 .0169 .0193 .2726 .0048 .0053 .5479 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >SKIN & PELAGE; SORE/SCAB; DORSALTHORACIC-RIGHT; VERIFIED AT NECROPSY: OBSERVED LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL -INFLAMMATION, SUBACUTE,-MINIMAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATELY SEVERE STOMACH, GL (ST) : -EDEMA,-MINIMAL -INFLAMMATION, ACUTE,-MINIMAL 3054 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1296 ANIMAL NUMBER: C92923 SEX: FEMALE DATE OF DEATH: 04/20/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/20/99 9:35 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 374.6 GRAMS PROSECTOR: TODD ACKER RECORDER: TONY HANFELD PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY SKIN (SK) : -CRUSTED AREA(S); RIGHT DORSAL LUMBAR REGION: SINGLE RED AND TAN AREA, 12 X 8 MM (CAP 20) CERVIX (CV) : -MASS(ES); RIGHT SIDE OF SEROSAL SURFACE, SINGLE MASS, 4 MM IN DIAMETER, TAN AND FIRM, SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (LUMBAR) NOT IDENTIFIED GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN SKIN (SK) : -ACANTHOSIS/HYPERKERATOSIS,-MINIMAL -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT -ULCERATION,-MODERATE MAMMARY, FEMALE (MF) : -SECRETORY MATERIAL,-PRESENT CERVIX (CV) : -CYST, SQUAMOUS,-PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 3055 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1297 ANIMAL NUMBER: C92923 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 374.6 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 9:35 PROSECTOR: TODD ACKER RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) 3056 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1298 ANIMAL NUMBER: C92924 SEX: FEMALE DATE OF DEATH: 11/29/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 11/29/99 15:04 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 589 STUDY WEEK OF DEATH: 85 TERMINAL BODY WEIGHT: 477.2 GRAMS PROSECTOR: TERRI L. WILLIAMS RECORDER: TONE EVERTS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); LIMB-HIND-LEFT; WEEK 83; 80 X 60MM; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) ADRENAL, CORTEX (AC) : -DARK FOCUS(I)/AREA(S); RIGHT, CORTEX: TWO BROWN SLIGHTLY RAISED AREAS, EACH IS 2 MM IN DIAMETER HISTOPATHOLOGY BRAIN (BR) : ^UNREMARKABLE LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, PERIPORTAL,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, PELVIC,-SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SLIGHT PANCREAS (PA) : -INFILTRATION, FAT, INTERSTITIAL,SLIGHT ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC / HEMORRHAGIC,MODERATE -PIGMENT,-MODERATE -B-ADENOMA,-NEOPLASM PRESENT 3057 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1299 ANIMAL NUMBER: C92924 SEX: FEMALE DATE OF DEATH: 11/29/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 11/29/99 15:04 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 589 STUDY WEEK OF DEATH: 85 TERMINAL BODY WEIGHT: 477.2 GRAMS PROSECTOR: TERRI L. WILLIAMS RECORDER: TONE EVERTS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY MUSCLE, SKELETAL (SM) : >NOTE:>THIGH MUSCLE: ROUTINE SECTION TAKEN FROM RIGHT THIGH, DUE TO MASS NERVE, SCIATIC (SN) : >NOTE:>ROUTINE SECTION TAKEN FROM RIGHT SIDE DUE TO MASS PITUITARY (PI) : -MASS(ES); 7 X 7 X 5 MM, RED, DARK RED, TAN, GREY, AND SOFT MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,-MODERATE MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,-SLIGHT THYMUS (TH) : >SECTION EXAMINED; TISSUE NOT PRESENT HEART (HT) : -CARDIOMYOPATHY,-MINIMAL NERVE, SCIATIC (SN) : >TISSUE MISSING PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL SKIN (SK) : -M-FIBROSARCOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT >NOTE:>ALSO SEE CLITORAL GLAND SEE SKIN FOR DIAGNOSIS OF MASS 3058 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1300 ANIMAL NUMBER: C92924 SEX: FEMALE DATE OF DEATH: 11/29/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 11/29/99 15:04 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 589 STUDY WEEK OF DEATH: 85 TERMINAL BODY WEIGHT: 477.2 GRAMS PROSECTOR: TERRI L. WILLIAMS RECORDER: TONE EVERTS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS(ES); LEFT INGUINAL AREA, EXTENDING OVER LEFT FLANK: SINGLE MASS, 70 X 70 X 55 MM, RED, DARK RED, TAN, LOBULATED, AND CYSTIC; SOME SECTIONS EXUDE A DARK RED FLUID; PORTION COLLECTED; REGIONAL LN (INGUINAL) NORMAL (CAP 21); OVERLYING SKIN HAS A SINGLE BLACK CRUSTED AREA, 40 X 25; COLLECTED INTACT WITH MASS -THICKENED; ALL REGIONS DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION -CYST(S); ALL REGIONS DIFFUSE: MULTIPLE TAN CREAMY FLUID FILLED CYSTS, UP TO 8 MM IN DIAMETER; COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN VAGINA (VA) : -HYPERPLASIA, MUCINOUS,-MODERATELY SEVERE CAUSE OF DEATH (CD) : -NEOPLASM, SKIN/MUSCLE/SUBCUTIS,PRESENT LN, INGUINAL (IN) : -HYPERPLASIA, LYMPHOPLASMACYTIC,-SLIGHT CLITORAL GLAND (CL) : -ECTASIA, DUCTAL,-PRESENT 3059 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1301 ANIMAL NUMBER: C92924 SEX: FEMALE DATE OF DEATH: 11/29/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 11/29/99 15:04 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 589 STUDY WEEK OF DEATH: 85 TERMINAL BODY WEIGHT: 477.2 GRAMS PROSECTOR: TERRI L. WILLIAMS RECORDER: TONE EVERTS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), HEMATO NEOPLASIA (HN) 3060 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1302 ANIMAL NUMBER: C92925 SEX: FEMALE DATE OF DEATH: 04/21/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/21/00 7:28 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 551.5 GRAMS PROSECTOR: TROY ELLENBECKER RECORDER: TONE EVERTS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); INGUINAL REGION-RIGHT; WEEK 84; 80 X 80MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); VENTRAL-ABDOMINAL; WEEK 92; 15 X 15MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); AXILLARY REGION-RIGHT; WEEK 99; 20 X 20MM; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; VENTRAL ABDOMINAL; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) HISTOPATHOLOGY BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-SLIGHT -HYPERPLASIA, BILE DUCT,-SLIGHT -FIBROSIS, PERIBILIARY,-SLIGHT -HYPERTROPHY, HEPATOCELLULAR, PERIPORTAL,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SLIGHT -MINERALIZATION, PELVIC,-MODERATE -HYPERPLASIA, TRANSITIONAL CELL,-SLIGHT SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATELY SEVERE PANCREAS (PA) : -HYPERTROPHY / BASOPHILIA, ACINAR CELL, MINIMAL ADRENAL, CORTEX (AC) : -PIGMENT,-SLIGHT 3061 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1303 ANIMAL NUMBER: C92925 SEX: FEMALE DATE OF DEATH: 04/21/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/21/00 7:28 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 551.5 GRAMS PROSECTOR: TROY ELLENBECKER RECORDER: TONE EVERTS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 10 X 8 X 6 MM, RED, DARK RED, TAN, AND SOFT STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: MULTIPLE BROWN LINEAR AREAS, UP TO 3 X 1 MM MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,MODERATELY SEVERE MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,-MODERATE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATE LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL HEART (HT) : -CARDIOMYOPATHY,-SLIGHT THYROID (TY) : -HYPERPLASIA, C-CELL,-SLIGHT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT -HYPERPLASIA,-SLIGHT HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL STOMACH, GL (ST) : >UNREMARKABLE MAMMARY, FEMALE (MF) : -B-FIBROADENOMA,- 2 NEOPLASMS PRESENT -B-ADENOMA,- 2 NEOPLASMS PRESENT 3062 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1304 ANIMAL NUMBER: C92925 SEX: FEMALE DATE OF DEATH: 04/21/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/21/00 7:28 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 551.5 GRAMS PROSECTOR: TROY ELLENBECKER RECORDER: TONE EVERTS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT AXILLARY REGION: SINGLE MASS, 25 X 15 X 10 MM, RED, TAN, AND SOFT; ON SECTION EXUDES A TAN CASEOUS SEMIFLUID (CAP 22); REGIONAL LN (AXILLARY) NORMAL (CAP 23) -MASS #2; LEFT INGUINAL REGION: SINGLE MASS, 35 X 20 X 10 MM, RED, TAN, AND FIRM; SAME ON CUT SURFACE (CAP 24); REGIONAL LN (INGUINAL) NOT IDENTIFIED -MASS #3; MID INGUINAL REGION EXTENDING TO PERIANAL REGION: SINGLE MASS, 110 X 85 X 30 MM (155.0 GRAMS), RED, DARK RED, AND FIRM; SAME ON CUT SURFACE; OVERLYING SKIN HAS MULTIPLE MOIST BROWN ERODED AREAS, UP TO 25 MM IN DIAMETER; COLLECTED WITH MASS; REGIONAL LN (INGUINAL) NOT IDENTIFIED LN, MANDIBULAR (MN) : -DIFFUSELY RED; RIGHT; COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN MAMMARY, FEMALE (MF) : >NOTE:>MASS #1 IS AN ADENOMA MASS #3 IS A FIBROADENOMA MASS #2 CONTAINS BOTH FIBROADENOMA AND ADENOMA VAGINA (VA) : -LUMEN, EXUDATE,-PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, AXILLARY (AX) : -HYPERPLASIA, LYMPHOPLASMACYTIC,MODERATE LN, MANDIBULAR (MN) : -HEMORRHAGE,-PRESENT 3063 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1305 ANIMAL NUMBER: C92925 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 551.5 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 7:28 PROSECTOR: TROY ELLENBECKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), TAIL (TI), LN, LUMBAr /i LIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), HEMATO NEOPLASIA (HN) 3064 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1306 ANIMAL NUMBER: C92926 SEX: FEMALE DATE OF DEATH: 05/15/98 STUDY DAY OF DATE AND TIME OF NECROPSY: 05/15/98 10:34 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 203.0 GRAMS PROSECTOR: JODI THIEREN RECORDER: DEBBIE PIRKEL PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN STATUS LF ADRENAL (AD0) RT ADRENAL (AD1) BRAIN (BR) LF KIDNEY (KD0) RT KIDNEY (KD1) LIVER (LI) LUNG (LU) LF OVARY (OV0) RT OVARY (OV1) SPLEEN (SP) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) UTERUS (UT) ------------------------------------ 7.4195 -------------------------------------------------- ------------------------------------ 3.6553 % -------------------------------------------------- -------------------------------------------------------------------------------------------- NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN WEIGHT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS 3065 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1307 ANIMAL NUMBER: C92926 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 203.0 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 10:34 PROSECTOR: JODI THIEREN RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) 3066 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1308 ANIMAL NUMBER: C92927 SEX: FEMALE DATE OF DEATH: 04/19/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/19/00 11:15 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 389.3 GRAMS PROSECTOR: JERALD W. JOHNSON RECORDER: TERRI L. WILLIAMS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); AXILLARY REGION-RIGHT; WEEK 88; 40 X 40MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); VENTRAL-THORACIC-RIGHT; WEEK 91; 80 X 50MM MASSES APPEAR TO HAVE GROWN TOGETHER; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); INGUINAL REGION-RIGHT; WEEK 94; 25 X 20MM; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; BLACK SKIN; VENTRALTHORACIC; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); INGUINAL REGION-LEFT; WEEK 105; 15 X 15MM; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) KIDNEY (KD) : -LARGE PELVIS(ES); BILATERAL -DIFFUSELY DARK; BILATERAL: DARK BROWN SPLEEN (SP) : -LARGE; 57 X 15 X 8 MM ADRENAL, CORTEX (AC) : -LARGE; LEFT: 9 X 7 X 7 MM (RIGHT IS 5 X 3 X 3 MM) -DIFFUSELY RED; LEFT: DARK RED HISTOPATHOLOGY BRAIN (BR) : -MINERALIZATION,-MINIMAL LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -HEMATOPOIESIS, EXTRAMEDULLARY,-SLIGHT -HYPERPLASIA, BILE DUCT,-SLIGHT -FIBROSIS, PERIBILIARY,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -DILATATION, PELVIC,-SEVERE -DEGENERATION TUBULAR,-SLIGHT SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SEVERE ADRENAL, CORTEX (AC) : -HYPERTROPHY, CORTICAL CELL,-MODERATELY SEVERE -DEGENERATION, CYSTIC / HEMORRHAGIC,SEVERE -PIGMENT,-MINIMAL 3067 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1309 ANIMAL NUMBER: C92927 SEX: FEMALE DATE OF DEATH: 04/19/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/19/00 11:15 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 389.3 GRAMS PROSECTOR: JERALD W. JOHNSON RECORDER: TERRI L. WILLIAMS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -LARGE; 7 X 5 X 5 MM; APPEARS SWOLLEN -RED FOCUS(I)/AREA(S); DIFFUSE: MULTIPLE DARK RED AREAS, UP TO 1 MM IN DIAMETER MAMMARY, FEMALE (MF) : -MASS(ES); VENTRAL THORACIC REGION: SINGLE MASS, 70 X 35 X 20 MM, RED, DARK RED, TAN, GREY, LOBULATED AND FIRM; SAME ON CUT SURFACE; OVERLYING SKIN HAS A SINGLE ULCERATED AREA, 50 X 30 MM; COLLECTED INTACT WITH MASS (CAP 22); REGIONAL LN (SEE AXILLARY) -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,-SEVERE MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,-SEVERE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATELY SEVERE HEART (HT) : -CARDIOMYOPATHY,-MINIMAL PITUITARY (PI) : -HYPERPLASIA,-MODERATE MAMMARY, FEMALE (MF) : -INFLAMMATION, CHRONIC-ACTIVE,MODERATELY SEVERE -B-FIBROADENOMA,- 2 NEOPLASMS PRESENT -HYPERPLASIA, GLANDULAR,-SLIGHT -FIBROSIS,-MODERATELY SEVERE >NOTE:>MASS #1 CONSISTS OF INFLAMMATION AND FIBROSIS 3068 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1310 ANIMAL NUMBER: C92927 SEX: FEMALE DATE OF DEATH: 04/19/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/19/00 11:15 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 389.3 GRAMS PROSECTOR: JERALD W. JOHNSON RECORDER: TERRI L. WILLIAMS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS #2; RIGHT AXILLARY REGION: SINGLE MASS, 50 X 50 X 35 MM, RED, TAN, GREY, FIRM AND LOBULATED; SAME ON CUT SURFACE; PORTION COLLECTED; REGIONAL LN (SEE AXILLARY) -MASS #3; RIGHT INGUINAL REGION AND LEFT PERIANAL REGION: TWO MASSES, RIGHT INGUINAL IS 40 X 25 X 20 MM, LEFT PERIANAL IS 25 X 20 X 15 MM, BOTH ARE RED, TAN, GREY AND FIRM; BOTH ARE SAME ON CUT SURFACE; RIGHT INGUINAL COLLECTED; REGIONAL LN (LEFT INGUINAL) NORMAL (CAP 23); RIGHT NOT IDENTIFIED LN, AXILLARY (AX) : -LARGE; BILATERAL: EACH IS 10 X 8 X 3 MM (CAP 24) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -GI TRACT-DARK FLUID CONTENT; DUODENUM, JEJUNUM, ILEUM, CECUM AND COLON: FILLED WITH A DARK GREEN SEMIFLUID MATERIAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, AXILLARY (AX) : -HYPERPLASIA, LYMPHOPLASMACYTIC,-SEVERE 3069 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1311 ANIMAL NUMBER: C92927 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 389.3 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 11:15 PROSECTOR: JERALD W. JOHNSON RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN), LN, INGUINAL (IN) 3070 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1312 ANIMAL NUMBER: C92928 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/15/99 STUDY DAY OF DEATH: 575 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: 460.4 GRAMS DATE AND TIME OF NECROPSY: 11/15/99 13:18 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >EYES; RED DISCHARGE; EYES; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; ATAXIC; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) HISTOPATHOLOGY EYE (EY) : -DYSTROPHY, CORNEAL IN EARLY STAGES, CONSISTS PRIMARILY OF FOCAL MINERALIZATION,-MINIMAL -INFLAMMATION, ACUTE,-MINIMAL BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -HEMORRHAGE,-MINIMAL LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-MODERATE -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT PANCREAS (PA) : -INFILTRATION, FAT, INTERSTITIAL,MINIMAL ADRENAL, CORTEX (AC) : -HYPERTROPHY, CORTICAL CELL,-SLIGHT 3071 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1313 ANIMAL NUMBER: C92928 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/15/99 STUDY DAY OF DEATH: 575 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: 460.4 GRAMS DATE AND TIME OF NECROPSY: 11/15/99 13:18 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) -MASS(ES); SINGLE MASS, 15 X 10 X 8 MM, DARK RED, RED, TAN AND SOFT STOMACH, NONGL (SU) -EROSION/ULCERATION; MUCOSA: SINGLE RED ERODED AREA, 10 X 4 MM LN, MANDIBULAR (MN) : -MOTTLED; LEFT: RED AND TAN; COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DARK RED AND DRY THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATELY SEVERE ESOPHAGUS (ES) : -HYPERKERATOSIS,-MODERATE THYROID (TY) : -HYPERPLASIA, C-CELL,-SLIGHT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, NONGL (SU) : -ACANTHOSIS / HYPERKERATOSIS,-SLIGHT -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT MAMMARY, FEMALE (MF) : -HYPERPLASIA, GLANDULAR,-SLIGHT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT LN, MANDIBULAR (MN) : -HYPERPLASIA, LYMPHOPLASMACYTIC,-SLIGHT 3072 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1314 ANIMAL NUMBER: C92928 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/15/99 STUDY DAY OF DEATH: 575 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: 460.4 GRAMS DATE AND TIME OF NECROPSY: 11/15/99 13:18 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) (SE), 3073 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1315 ANIMAL NUMBER: C92929 SEX: FEMALE DATE OF DEATH: 06/08/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 06/08/99 14:55 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 415 STUDY WEEK OF DEATH: 60 TERMINAL BODY WEIGHT: 279.4 GRAMS PROSECTOR: GREGORY CODDINGTON RECORDER: JARED FISHEL PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; HEAD TILT; LEFT; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: NOT OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 13 X 8 X 8 MM, RED, DARK RED, TAN AND SOFT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; RIGHT UPPER INCISOR HISTOPATHOLOGY BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MINIMAL ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-MINIMAL -HYPERTROPHY, CORTICAL CELL,-MINIMAL MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,-SLIGHT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 3074 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1316 ANIMAL NUMBER: C92929 SEX: FEMALE DATE OF DEATH: 06/08/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 06/08/99 14:55 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 415 STUDY WEEK OF DEATH: 60 TERMINAL BODY WEIGHT: 279.4 GRAMS PROSECTOR: GREGORY CODDINGTON RECORDER: JARED FISHEL PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) (SM), 3075 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1317 ANIMAL NUMBER: C92930 SEX: FEMALE DATE OF DEATH: 04/20/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/20/99 9:35 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 631.8 GRAMS PROSECTOR: GREGORY CODDINGTON RECORDER: TONY HANFELD PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) LF ADRENAL (AD0) RT ADRENAL (AD1) BRAIN (BR) LF KIDNEY (KD0) RT KIDNEY (KD1) LIVER (LI) LUNG (LU) LF OVARY (OV0) RT OVARY (OV1) SPLEEN (SP) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) UTERUS (UT) .0457 .0386 2.1404 1.8673 1.9836 18.5142 1.5595 .0605 .0605 .6350 .0206 .0220 .9256 .0072 % .0061 % .3388 % .2956 % .3140 % 2.9304 % .2468 % .0096 % .0096 % .1005 % .0033 % .0035 % .1465 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0214 .0180 1.0000 .8724 .9267 8.6499 .7286 .0283 .0283 .2967 .0096 .0103 .4324 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >SKIN & PELAGE; SORE/SCAB; DORSALTHORACIC-RIGHT; VERIFIED AT NECROPSY: OBSERVED BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT >NOTE:>SOME EXTENSION OF THE PITUITARY MASS INTO WHAT APPEARS TO BE A RECESS OF THE PIA MATER. THIS WAS NOT INTERPRETED TO BE ACTUAL INVASION OF THE BRAIN. LIVER (LI) : -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-SLIGHT 3076 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1318 ANIMAL NUMBER: C92930 SEX: FEMALE DATE OF DEATH: 04/20/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/20/99 9:35 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 631.8 GRAMS PROSECTOR: GREGORY CODDINGTON RECORDER: TONY HANFELD PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -LARGE; 8 X 7 X 5 MM; APPEARS SWOLLEN SKIN (SK) : -CRUSTED AREA(S); RIGHT DORSAL LUMBAR: SINGLE TAN, DRY CRUSTED AREA, 23 X 7 MM (CAP 21) -LIGHT AREA(S)/FOCUS(I); MID DORSAL LUMBAR REGION: SINGLE TAN AREA, 25 X 7 MM (CAP 22) KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SLIGHT -MINERALIZATION, PELVIC,-MODERATE -MINERALIZATION, TUBULAR,-MINIMAL -PIGMENT, TUBULAR,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : -INFLAMMATION, ACUTE,-MINIMAL SKIN (SK) : -ACANTHOSIS/HYPERKERATOSIS,-SLIGHT -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT -ULCERATION,-SLIGHT -HEMORRHAGE,-PRESENT -DEGENERATION, MUSCLE, SUBCUTANEOUS,SLIGHT 3077 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1319 ANIMAL NUMBER: C92930 SEX: FEMALE DATE OF DEATH: 04/20/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/20/99 9:35 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 631.8 GRAMS PROSECTOR: GREGORY CODDINGTON RECORDER: TONY HANFELD PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -CYST(S); ALL REGIONS, DIFFUSE: MULTIPLE TAN CREAMY SEMIFLUID FILLED CYSTS, UP TO 12 X 10 X 8 MM (CAP 20) GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT -SECRETORY MATERIAL,-PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 3078 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1320 ANIMAL NUMBER: C92930 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 631.8 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 9:35 PROSECTOR: GREGORY CODDINGTON RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON(CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART(HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 3079 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1321 ANIMAL NUMBER: C92931 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 08/22/99 STUDY DAY OF DEATH: 490 STUDY WEEK OF DEATH: 70 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 08/22/99 14:45 PROSECTOR: KEVIN BILLINGS RECORDER: KEVIN BILLINGS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); AXILLARY REGION-RIGHT; WEEK 69; 15 X 15MM; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) HISTOPATHOLOGY BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -CONGESTION,-PRESENT -VACUOLATION, HEPATOCELLULAR,-MODERATE KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATE -HYPERTROPHY, CORTICAL CELL,-MINIMAL THYMUS (TH) : -HEMORRHAGE,-PRESENT -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),SLIGHT LUNG (LU) : -CONGESTION,-PRESENT 3080 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1322 ANIMAL NUMBER: C92931 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 08/22/99 STUDY DAY OF DEATH: 490 STUDY WEEK OF DEATH: 70 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 08/22/99 14:45 PROSECTOR: KEVIN BILLINGS RECORDER: KEVIN BILLINGS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); SINGLE MASS, 8 X 6 X 6 RED, GRAY, SOFT AND LOBULATED MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT AXILLARY REGION: SINGLE MASS, 15 X 10 X 8 MM, RED, AND FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (AXILLARY) NORMAL (CAP 21) MM, TAN HEART (HT) : -CARDIOMYOPATHY,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -B-ADENOMA,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 3081 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1323 ANIMAL NUMBER: C92931 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 08/22/99 STUDY DAY OF DEATH: 490 STUDY WEEK OF DEATH: 70 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 08/22/99 14:45 PROSECTOR: KEVIN BILLINGS RECORDER: KEVIN BILLINGS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG), GENERAL COMMENT (GC) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN), LN, AXILLARY (AX) 3082 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1324 ANIMAL NUMBER: C92932 SEX: FEMALE DATE OF DEATH: 01/29/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 01/29/99 15:03 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 285 STUDY WEEK OF DEATH: 41 TERMINAL BODY WEIGHT: 260.1 GRAMS PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK 18; 45 X 35MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); VENTRAL-ABDOMINAL-RIGHT; WEEK 25; 50 X 50MM; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; PALE; ENTIRE BODY; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; SORE/SCAB; VENTRALABDOMINAL-LEFT; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY ADRENAL, CORTEX (AC) : -LARGE; BILATERAL: EACH IS 6 MM IN DIAMETER HISTOPATHOLOGY LIVER (LI) : -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, PERIPORTAL,-MODERATE KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL -REGENERATION, TUBULAR EPITHELIUM,MINIMAL -VACUOLATION, TUBULAR EPITHELIUM,MINIMAL -KARYOMEGALY, TUBULAR CELL,-SLIGHT SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATE ADRENAL, MEDULLA (MA) : -HYPERPLASIA, MEDULLARY,-MINIMAL LN, MESENTERIC (MS) : -HYPERPLASIA, LYMPHOPLASMACYTIC,MODERATE MARROW, FEMUR (FM) : -HYPERPLASIA, ERYTHROID, MARROW,MODERATE 3083 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1325 ANIMAL NUMBER: C92932 SEX: FEMALE DATE OF DEATH: 01/29/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 01/29/99 15:03 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 285 STUDY WEEK OF DEATH: 41 TERMINAL BODY WEIGHT: 260.1 GRAMS PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -LARGE; 5 X 5 X 3 MM, APPEARS SWOLLEN -DARK FOCUS(I)/AREA(S); MID POSTERIOR ASPECT: SINGLE GREY FOCUS, 1 MM IN DIAMETER MAMMARY, FEMALE (MF) : -MASS(ES); ENTIRE ABDOMINAL REGION, EXTENDING TO VULVA AND LEFT HIND LEG: SINGLE MASS, 90 X 50 X 30 MM, RED, DARK RED, TAN, BROWN, FIRM, AND LOBULATED; SAME ON CUT SURFACE; ON SECTION, SOME AREAS EXUDED A DARK BROWN SEMIFLUID; COLLECTED; OVERLYING SKIN HAS MULTIPLE DARK RED AND BROWN ABRADED AREAS, UP TO 40 X 20 MM; COLLECTED INTACT WITH MASS; REGIONAL LN (INGUINAL) NORMAL VAGINA (VA) : -SEMIFLUID MATERIAL; TAN AND MUCOID LN, INGUINAL (IN) : >NOTE:>INGUINAL LYMPH NODE THAT IS REGIONAL TO MAMMARY GLAND MASS IS COLLECTED (CAP 20) THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY) MODERATELY SEVERE PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT -ANGIECTASIS,-PRESENT MAMMARY, FEMALE (MF) : -M-CARCINOMA,-NEOPLASM PRESENT -CYST / GALACTOCELE,-PRESENT -INFLAMMATION, CHRONIC-ACTIVE,MODERATELY SEVERE VAGINA (VA) : -LUMEN, EXUDATE,-PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, MAMMARY GLAND,-PRESENT LN, INGUINAL (IN) : -HYPERPLASIA, LYMPHOPLASMACYTIC,MODERATE 3084 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1326 ANIMAL NUMBER: C92932 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/29/99 STUDY DAY OF DEATH: 285 STUDY WEEK OF DEATH: 41 TERMINAL BODY WEIGHT: 260.1 GRAMS DATE AND TIME OF NECROPSY: 01/29/99 15:03 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUm /s KULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), OVARY (OV), BONE, FEMUR (FE), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), HEMATO NEOPLASIA (HN) 3085 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1327 ANIMAL NUMBER: C92933 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/21/00 STUDY DAY OF DEATH: 642 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: 541.0 GRAMS DATE AND TIME OF NECROPSY: 01/21/00 14:38 PROSECTOR: DAVID SCHUETTE RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); AXILLARY REGION-LEFT; WEEK 64; 50 X 50MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); INGUINAL REGION-RIGHT; WEEK 74; 70 X 70MM; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: NOT OBSERVED >SKIN & PELAGE; SORE/SCAB; VENTRALABDOMINAL-RIGHT; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; BLACK SKIN; VENTRALABDOMINAL-RIGHT; VERIFIED AT NECROPSY: NOT APPLICABLE >EXCRETION; NO FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >RESPIRATION; LABORED; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; COLD TO TOUCH; ENTIRE BODY; VERIFIED AT NECROPSY: NOT APPLICABLE >EYES; PALE; EYES; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE AREAS, UP TO 5 X 4 MM; COLLECTED ON ROUTINE SECTIONS ADRENAL, CORTEX (AC) : -LARGE; LEFT: 8 X 5 X 5 MM (RIGHT IS 5 X 4 X 3 MM) HISTOPATHOLOGY EYE (EY) : -INFLAMMATION, ACUTE,-SLIGHT BRAIN (BR) : >UNREMARKABLE LIVER (LI) : -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL -HEMATOPOIESIS, EXTRAMEDULLARY,-SLIGHT -HYPERPLASIA, BILE DUCT,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MODERATE -MINERALIZATION, PELVIC,-SEVERE -MINERALIZATION, TUBULAR,-MODERATE -HYPERPLASIA, TRANSITIONAL CELL,MODERATE SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SEVERE PANCREAS (PA) : -HYPERTROPHY / BASOPHILIA, ACINAR CELL,MINIMAL ADRENAL, CORTEX (AC) : -HYPERTROPHY, CORTICAL CELL,-SEVERE 3086 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1328 ANIMAL NUMBER: C92933 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/21/00 STUDY DAY OF DEATH: 642 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: 541.0 GRAMS DATE AND TIME OF NECROPSY: 01/21/00 14:38 PROSECTOR: DAVID SCHUETTE RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -LARGE; 7 X 6 X 4 MM -MOTTLED; BROWN, DARK BROWN AND TAN ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC / HEMORRHAGIC,SEVERE -PIGMENT,-SLIGHT MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,MODERATELY SEVERE MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,MODERATELY SEVERE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATE LUNG (LU) : -HYPERPLASIA, TYPE II PNEUMOCYTE,SLIGHT -MINERALIZATION, SEPTAL,-MINIMAL HEART (HT) : -CARDIOMYOPATHY,-MODERATELY SEVERE TRACHEA (TR) : -EXUDATE, LUMINAL,-PRESENT -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT PARATHYROID (PT) : -HYPERPLASIA,-MODERATE PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT -CYST,-PRESENT 3087 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1329 ANIMAL NUMBER: C92933 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/21/00 STUDY DAY OF DEATH: 642 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: 541.0 GRAMS DATE AND TIME OF NECROPSY: 01/21/00 14:38 PROSECTOR: DAVID SCHUETTE RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY STOMACH, GL (ST) : -EROSION/ULCERATION; MUCOSA: SINGLE DARK RED ULCERATED AREA, 4 X 2 MM MAMMARY, FEMALE (MF) : -MASS(ES); LEFT AXILLARY REGION: SINGLE MASS, 50 X 40 X 25 MM, TAN, FIRM AND LOBULATED; SAME ON CUT SURFACE; SOME SECTIONS EXUDE A TAN CREAMY SEMIFLUID MATERIAL; COLLECTED INTACT; REGIONAL LN (SEE AXILLARY) -THICKENED; ALL REGIONS, DIFFUSE: TAN; COLLECTED ON ROUTINE SECTION -MASS #2; RIGHT INGUINAL REGION, EXTENDING TO MID VENTRAL ABDOMINAL REGION: SINGLE MASS, 70 X 70 X 25 MM, DARK BROWN, BROWN, TAN, FIRM AND LOBULATED; SAME ON CUT SURFACE; ON SECTION EXUDES A DARK BROWN FLUID MATERIAL; OVERLYING SKIN HAS MULTIPLE DRY DARK BROWN CRUSTED AREAS, UP TO 6 MM IN DIAMETER; COLLECTED INTACT; REGIONAL LN (SEE AXILLARY) HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL STOMACH, GL (ST) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT -MINERALIZATION, GLANDULAR MUCOSA,MODERATELY SEVERE MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT -B-FIBROADENOMA,-NEOPLASM PRESENT -B-ADENOMA,-NEOPLASM PRESENT >NOTE:>MASS 2 IS THE FIBROADENOMA CAUSE OF DEATH (CD) : -NEOPLASM, MAMMARY GLAND,-PRESENT >NOTE:>CAUSE OF DEATH IS THE ADENOMA 3088 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1330 ANIMAL NUMBER: C92933 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/21/00 STUDY DAY OF DEATH: 642 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: 541.0 GRAMS DATE AND TIME OF NECROPSY: 01/21/00 14:38 PROSECTOR: DAVID SCHUETTE RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY LN, AXILLARY (AX) : -MOTTLED; BILATERAL: BROWN AND TAN (CAP 22) LN, MANDIBULAR (MN) : -LARGE; RIGHT: 15 X 8 X 5 MM (LEFT IS 5 X 3 X 2 MM) (CAP 21) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: MOIST, BROWN MATERIAL LN, AXILLARY (AX) : -HYPERPLASIA, LYMPHOPLASMACYTIC,MODERATE LN, MANDIBULAR (MN) : -HYPERPLASIA, LYMPHOPLASMACYTIC,MODERATELY SEVERE -ABSCESS,-MODERATELY SEVERE 3089 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1331 ANIMAL NUMBER: C92933 SEX: FEMALE DATE OF DEATH: 01/21/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 01/21/00 14:38 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 642 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: 541.0 GRAMS PROSECTOR: DAVID SCHUETTE RECORDER: TERRI L. WILLIAMS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP),JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA),LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: ADRENAL, MEDULLA (MA),LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM),NERVE, SCIATIC (SN), ESOPHAGUS (ES), THYROID (TY), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 3090 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1332 ANIMAL NUMBER: C92934 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/13/99 STUDY DAY OF DEATH: 603 STUDY WEEK OF DEATH: 87 TERMINAL BODY WEIGHT: 327.4 GRAMS DATE AND TIME OF NECROPSY: 12/13/99 8:05 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; MISSING; TEETH; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; HEAD TILT; RIGHT; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; ATAXIC; VERIFIED AT NECROPSY: NOT APPLICABLE >RESPIRATION; LABORED; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 9 X 8 X 8 MM, DARK RED AND SOFT HISTOPATHOLOGY BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -LIPIDOSIS, SUBCAPSULAR (TENSION LIPIDOSIS),-SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT PANCREAS (PA) : -ATROPHY, ACINAR,-SLIGHT -HYPERTROPHY / BASOPHILIA, ACINAR CELL,MINIMAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATE -PIGMENT,-MINIMAL LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-MODERATE PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL 3091 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1333 ANIMAL NUMBER: C92934 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/13/99 STUDY DAY OF DEATH: 603 STUDY WEEK OF DEATH: 87 TERMINAL BODY WEIGHT: 327.4 GRAMS DATE AND TIME OF NECROPSY: 12/13/99 8:05 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY LN, MANDIBULAR (MN) : -LARGE; RIGHT: UP TO 4 X 4 X 3 MM (LEFT IS UP TO 3 X 2 X 2 MM) (CAP 21) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL LOWER INCISORS -TEETH MISSING/BROKEN/WORN; RIGHT UPPER INCISOR: BROKEN OFF AT BASE -DISCHARGE-OCULAR; BILATERAL: DRY, RED MATERIAL -ANIMAL THIN -DISCHARGE-NASAL; DRY, RED MATERIAL MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT LN, MANDIBULAR (MN) : -HYPERPLASIA, LYMPHOPLASMACYTIC,MODERATE 3092 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1334 ANIMAL NUMBER: C92934 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/13/99 STUDY DAY OF DEATH: 603 STUDY WEEK OF DEATH: 87 TERMINAL BODY WEIGHT: 327.4 GRAMS DATE AND TIME OF NECROPSY: 12/13/99 8:05 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), KIDNEY (KD), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), SALIV GL, MANDIB STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) (SG), 3093 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1335 ANIMAL NUMBER: C92935 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/22/99 STUDY DAY OF DEATH: 612 STUDY WEEK OF DEATH: 88 TERMINAL BODY WEIGHT: 356.3 GRAMS DATE AND TIME OF NECROPSY: 12/22/99 11:11 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; HUNCHED; VERIFIED AT NECROPSY: NOT APPLICABLE >EYES; RED DISCHARGE; EYE-RIGHT; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; ATAXIC; VERIFIED AT NECROPSY: NOT APPLICABLE >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -RED FOCUS(I)/AREA(S); LEFT LATERAL LOBE: SINGLE DARK RED AREA, 5 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION ADRENAL, CORTEX (AC) : -LARGE; RIGHT: 13 X 10 X 10 MM (LEFT IS 4 X 3 X 3 MM) HISTOPATHOLOGY BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MODERATE KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MINIMAL ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC / HEMORRHAGIC,SEVERE -PIGMENT,-SLIGHT ADRENAL, MEDULLA (MA) : -UNILATERALLY EXAMINED,-PRESENT 3094 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1336 ANIMAL NUMBER: C92935 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/22/99 STUDY DAY OF DEATH: 612 STUDY WEEK OF DEATH: 88 TERMINAL BODY WEIGHT: 356.3 GRAMS DATE AND TIME OF NECROPSY: 12/22/99 11:11 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 11 X 10 X 7 MM, RED, DARK RED, GRAY, AND SOFT UTERUS (UT) : -THICKENED WALL; BILATERAL, DIFFUSE -MASS(ES); MID RIGHT HORN: SINGLE PEDUNCULATED MASS, 9 X 6 X 6 MM, TAN, LOBULATED, AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (LUMBAR) NORMAL (CAP 21) THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),SEVERE LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL MAMMARY, FEMALE (MF) : -B-FIBROADENOMA,-NEOPLASM PRESENT UTERUS (UT) : -DILATATION,-SLIGHT -B-LEIOMYOMA,-NEOPLASM PRESENT VAGINA (VA) : -LUMEN, EXUDATE,-PRESENT -HYPERPLASIA, MUCINOUS,-MODERATE CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT LN, LUMBAR/ILIAC (LM) : -HYPERPLASIA, LYMPHOPLASMACYTIC,MODERATE 3095 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1337 ANIMAL NUMBER: C92935 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/22/99 STUDY DAY OF DEATH: 612 STUDY WEEK OF DEATH: 88 TERMINAL BODY WEIGHT: 356.3 GRAMS DATE AND TIME OF NECROPSY: 12/22/99 11:11 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; RIGHT: DARK RED AND MOIST THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL(ML), LN, AXILLARY(AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUm /s KULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), HEMATO NEOPLASIA (HN) (SE), 3096 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1338 ANIMAL NUMBER: C92936 SEX: FEMALE DATE OF DEATH: 04/20/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/20/00 7:30 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 328.1 GRAMS PROSECTOR: CHANESTA ACKER RECORDER: TONE EVERTS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); AXILLARY REGION-LEFT; WEEK 63; 50 X 40MM; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 11 X 10 X 6 MM, DARK RED, TAN, SOFT, AND LOBULATED STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA: MULTIPLE DARK BROWN ERODED AREAS, UP TO 3 MM IN DIAMETER HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-SLIGHT -FIBROSIS, PERIBILIARY,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, PELVIC,-MODERATE -HYPERPLASIA, TRANSITIONAL CELL,-SLIGHT THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, NONGL (SU) : -ACANTHOSIS / HYPERKERATOSIS,MODERATELY SEVERE -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT 3097 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1339 ANIMAL NUMBER: C92936 SEX: FEMALE DATE OF DEATH: 04/20/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/20/00 7:30 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 328.1 GRAMS PROSECTOR: CHANESTA ACKER RECORDER: TONE EVERTS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS(ES); LEFT AXILLARY REGION: SINGLE MASS, 55 X 35 X 22 MM, TAN, FIRM, AND LOBULATED; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (AXILLARY) NORMAL (CAP 23) -THICKENED; ALL REGIONS DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN GENERAL INFORMATION (XX) : >NOTE:>ANIMAL NECROPSIED OUT OF ORDER DUE TO MORIBUND CONDITION FOLLOWING BLOOD COLLECTION MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT -B-FIBROADENOMA,-NEOPLASM PRESENT -HYPERPLASIA, GLANDULAR,-SLIGHT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, AXILLARY (AX) : -HYPERPLASIA, LYMPHOPLASMACYTIC,MODERATELY SEVERE -INFILTRATE, MACROPHAGE, PIGMENTED,MODERATE 3098 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1340 ANIMAL NUMBER: C92936 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 328.1 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 7:30 PROSECTOR: CHANESTA ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), LUNG (LU), PARATHYROID (PT), URINARY BLADDER (UB) 3099 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1341 ANIMAL NUMBER: C92937 SEX: FEMALE DATE OF DEATH: 07/13/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 07/13/99 9:45 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 450 STUDY WEEK OF DEATH: 65 TERMINAL BODY WEIGHT: 270.4 GRAMS PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); VENTRAL-ABDOMINAL; WEEK 61; 15 X 15MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); AXILLARY REGION-LEFT; WEEK 64; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; HUNCHED; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 15 X 10 X 10 MM, DARK RED, TAN, AND SOFT HISTOPATHOLOGY BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT KIDNEY (KD) : -MINERALIZATION, PELVIC,-SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT PANCREAS (PA) : -HYPERTROPHY / BASOPHILIA, ACINAR CELL,MINIMAL -VACUOLATION, FOCAL,-MINIMAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL 3100 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1342 ANIMAL NUMBER: C92937 SEX: FEMALE DATE OF DEATH: 07/13/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 07/13/99 9:45 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 450 STUDY WEEK OF DEATH: 65 TERMINAL BODY WEIGHT: 270.4 GRAMS PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -CYST(S); ALL REGIONS, DIFFUSE: MULTIPLE TAN, CREAMY SEMIFLUID FILLED CYSTS, UP TO 15 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION AND CAP 20; POSSIBLY CONSISTENT WITH MASSES NOTED IN CLINICAL OBSERVATIONS GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL, UPPER INCISORS -DISCHARGE-OCULAR; BILATERAL: DARK RED AND DRY -ANIMAL THIN -DISCHARGE-NASAL; DARK RED AND DRY CERVIX (CV) : >TISSUE MISSING VAGINA (VA) : -LUMEN, EXUDATE,-PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 3101 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1343 ANIMAL NUMBER: C92937 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/13/99 STUDY DAY OF DEATH: 450 STUDY WEEK OF DEATH: 65 TERMINAL BODY WEIGHT: 270.4 GRAMS DATE AND TIME OF NECROPSY: 07/13/99 9:45 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), HEMATO NEOPLASIA (HN) 3102 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1344 ANIMAL NUMBER: C92938 SEX: FEMALE DATE OF DEATH: 04/20/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/20/00 10:32 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 650.9 GRAMS PROSECTOR: TODD ACKER RECORDER: TONE EVERTS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; SORE/SCAB; PAWS-HIND; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; SWOLLEN; PAW-HIND-RIGHT; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY ADRENAL, CORTEX (AC) : -LARGE; RIGHT: 8 X 5 X 5 MM (LEFT IS 5 X 4 X 4 MM) PITUITARY (PI) : -MASS(ES); 6 X 6 X 4 MM, RED, DARK RED, GREY, AND SOFT MAMMARY, FEMALE (MF) : -CYST(S); MID PERINEAL REGION: MULTIPLE TAN CREAMY SEMIFLUID FILLED CYSTS, UP TO 15 X 10 MM (CAP 22) HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -NECROSIS, INDIVIDUAL HEPATOCYTE,MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, PELVIC,-SLIGHT ADRENAL, CORTEX (AC) : -HYPERTROPHY, CORTICAL CELL,-MODERATELY SEVERE -DEGENERATION, CYSTIC / HEMORRHAGIC,MODERATELY SEVERE -PIGMENT,-MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PITUITARY (PI) : -HYPERPLASIA,-MODERATELY SEVERE MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT -HYPERPLASIA, GLANDULAR,-MINIMAL 3103 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1345 ANIMAL NUMBER: C92938 SEX: FEMALE DATE OF DEATH: 04/20/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/20/00 10:32 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 650.9 GRAMS PROSECTOR: TODD ACKER RECORDER: TONE EVERTS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY FOOT/FOOTPAD (FP) : -CRUSTED AREA(S); BILATERAL HINDFEET: SINGLE RED, TAN, AND MOIST AREA ON EACH, LEFT IS 5 X 3 MM, RIGHT IS 17 X 13 X 3 MM, SURROUNDING TISSUE APPEARS SWOLLEN; BOTH COLLECTED ADIPOSE TISSUE (AT) : -GELATINOUS; TISSUE SURROUNDING BOTH UTERINE HORNS, AND BOTH KIDNEYS: CLEAR AND YELLOW (CAP 23) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -ANIMAL OBESE CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT CAVITY, ABDOM (PC) : -INFLAMMATION / NECROSIS, FAT,-MODERATE FOOT/FOOTPAD (FP) : -INFLAMMATION, CHRONIC-ACTIVE,-SEVERE -ULCERATION,-SEVERE -ACANTHOSIS / HYPERKERATOSIS,-SLIGHT ADIPOSE TISSUE (AT) : >NOT REQUIRED TO BE EXAMINED FOR ANIMAL >NOTE:>SEE PERITONEAL CAVITY FOR DIAGNOSIS OF MASS 3104 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1346 ANIMAL NUMBER: C92938 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 650.9 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 10:32 PROSECTOR: TODD ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), ADRENAL, MEDULLA (MA), LUNG (LU), PARATHYROID (PT), URINARY BLADDER (UB) 3105 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1347 ANIMAL NUMBER: C92939 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/23/99 STUDY DAY OF DEATH: 491 STUDY WEEK OF DEATH: 71 TERMINAL BODY WEIGHT: 351.3 GRAMS DATE AND TIME OF NECROPSY: 08/23/99 14:44 PROSECTOR: DAVID SCHUETTE RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); VENTRAL-CERVICAL-RIGHT; WEEK 47; 30 X 60MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); VENTRAL-ABDOMINAL-RIGHT; WEEK 63; 15 X 15MM; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; VENTRALTHORACIC; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); AXILLARY REGION-LEFT; WEEK 58; 30 X 30MM; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL -MINERALIZATION, TUBULAR,-MINIMAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : -ANGIECTASIS.-SLIGHT -PIGMENT,-MINIMAL MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,-SLIGHT MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,MODERATELY SEVERE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MINIMAL LUNG (LU) : -N-CARCINOMA,-FROM MAMMARY, FEMALE 3106 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1348 ANIMAL NUMBER: C92939 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/23/99 STUDY DAY OF DEATH: 491 STUDY WEEK OF DEATH: 71 TERMINAL BODY WEIGHT: 351.3 GRAMS DATE AND TIME OF NECROPSY: 08/23/99 14:44 PROSECTOR: DAVID SCHUETTE RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY LUNG (LU) : -LIGHT FOCUS(I)/AREA(S); TRIMMING COMMENT: RIGHT CRANIAL LOBE: SINGLE TAN AREA, 3 X 4 MM; TRIMMED IN BLOCK 11 WITH ROUTINE SECTIONS PITUITARY (PI) : -MASS(ES); 6 MM IN DIAMETER, DARK RED AND SOFT; PORTION COLLECTED ON BRAIN MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT SHOULDER REGION: SINGLE MASS, 60 X 40 X 25 MM, TAN, RED, LOBULATED, FIRM AND VASCULAR; SAME ON CUT SURFACE; SKIN OVERLYING MASS HAS DARK RED AND BROWN ULCERATED AREA, 30 X 20 MM; COLLECTED INTACT; REGIONAL LN (AXILLARY) NORMAL (CAP 20) -MASS #2; LEFT AXILLARY REGION: SINGLE MASS, 35 X 30 X 20 MM, TAN, DARK RED, RED AND LOBULATED; SAME ON CUT SURFACE; SOME SECTIONS EXUDE A TAN GELATINOUS SEMISOLID MATERIAL; COLLECTED INTACT; REGIONAL LN (AXILLARY) NORMAL (CAP 20) -MASS #3; RIGHT VENTRAL ABDOMINAL: SINGLE MASS, 30 X 30 X 15 MM, TAN AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (INGUINAL) NOT IDENTIFIED PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -M-CARCINOMA,- 3 NEOPLASMS PRESENT -CYST / GALACTOCELE,-PRESENT -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT 3107 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1349 ANIMAL NUMBER: C92939 SEX: FEMALE DATE OF DEATH: 08/23/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 08/23/99 14:44 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 491 STUDY WEEK OF DEATH: 71 TERMINAL BODY WEIGHT: 351.3 GRAMS PROSECTOR: DAVID SCHUETTE RECORDER: JARED FISHEL PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY UTERUS (UT) : -CYST(S); BILATERAL, WITHIN WALLS: MULTIPLE CLEAR FLUID FILLED CYSTS, UP TO 2 MM IN DIAMETER GENERAL COMMENT (GC) : -BLOOD FILM TAKEN GENERAL INFORMATION (XX) : >NOTE:>ANIMAL INADVERTENTLY SACRIFICED WITH SODIUM PENTOBARBITAL UTERUS (UT) : -DILATATION,-MODERATELY SEVERE CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT LN, AXILLARY (AX) : -HEMORRHAGE,-PRESENT -HYPERPLASIA, LYMPHOPLASMACYTIC,-SLIGHT 3108 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1350 ANIMAL NUMBER: C92939 SEX: FEMALE DATE OF DEATH: 08/23/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 08/23/99 14:44 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 491 STUDY WEEK OF DEATH: 71 TERMINAL BODY WEIGHT: 351.3 GRAMS PROSECTOR: DAVID SCHUETTE RECORDER: JARED FISHEL PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUm /s KULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 3109 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1351 ANIMAL NUMBER: C92940 SEX: FEMALE DATE OF DEATH: 12/13/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 12/13/99 13:31 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 603 STUDY WEEK OF DEATH: 87 TERMINAL BODY WEIGHT: 587.6 GRAMS PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); AXILLARY REGION-LEFT; WEEK 50; 100 X 90MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); INGUINAL REGION-LEFT; WEEK 73; 60 X 70MM; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY ADRENAL, CORTEX (AC) : -LARGE; LEFT: 9 X 5 X 5 MM (RIGHT IS 5 X 3 X 3 MM) -IRREGULAR SHAPE; LEFT: LOBULATED PITUITARY (PI) : -DARK FOCUS(I)/AREA(S); MID ASPECT: SINGLE DARK BROWN FOCUS, 1 MM IN DIAMETER HISTOPATHOLOGY LIVER (LI) : -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : -HYPERTROPHY, CORTICAL CELL,-MODERATE -DEGENERATION, CYSTIC / HEMORRHAGIC,SEVERE MARROW, FEMUR (FM) : -HYPERPLASIA, ERYTHROID, MARROW,-SLIGHT MARROW, STERNUM (SE) : -HYPERPLASIA, ERYTHROID, MARROW,-SLIGHT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT 3110 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1352 ANIMAL NUMBER: C92940 SEX: FEMALE DATE OF DEATH: 12/13/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 12/13/99 13:31 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 603 STUDY WEEK OF DEATH: 87 TERMINAL BODY WEIGHT: 587.6 GRAMS PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY STOMACH, GL (ST) : -LIGHT FOCUS(I)/AREA(S); MUCOSA: MULTIPLE GREY, INDISTINCT AREAS MAMMARY, FEMALE (MF) : -MASS(ES); LEFT INGUINAL REGIONAL: SINGLE MASS, 80 X 61 X 55 MM, RED, DARK RED, GREY, TAN, LOBULATED, VASCULAR, AND FIRM; SOME SECTIONS EXUDE A TAN, CREAMY SEMIFLUID MATERIAL; COLLECTED INTACT; REGIONAL LN (INGUINAL) NOT IDENTIFIED -MASS #2; LEFT AXILLARY REGION: SINGLE MASS, 128 X 91 X 73 MM (199.0 GRAMS), RED, DARK RED, GREY, TAN, VASCULAR, LOBULATED AND FIRM; SOME SECTIONS EXUDE A TAN, CREAMY SEMIFLUID MATERIAL; PORTION COLLECTED; REGIONAL LN (SEE AXILLARY) -MASS #3; LEFT LATERAL THORACIC REGION: SINGLE MASS, 15 X 11 X 9 MM, DARK BROWN, LOBULATED AND SOFT; SAME ON CUT SURFACE (CAP 22); REGIONAL LN (INGUINAL) NOT IDENTIFIED LN, AXILLARY (AX) : -MOTTLED; LEFT: RED AND TAN (CAP 21) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN STOMACH, GL (ST) : >UNREMARKABLE MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT -B-FIBROADENOMA,- 2 NEOPLASMS PRESENT -B-ADENOMA,-NEOPLASM PRESENT >NOTE:>MASS #3 IS THE ADENOMA CAUSE OF DEATH (CD) : -NEOPLASM, MAMMARY GLAND,-PRESENT LN, AXILLARY (AX) : -HEMORRHAGE,-PRESENT 3111 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1353 ANIMAL NUMBER: C92940 SEX: FEMALE DATE OF DEATH: 12/13/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 12/13/99 13:31 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 603 STUDY WEEK OF DEATH: 87 TERMINAL BODY WEIGHT: 587.6 GRAMS PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -ANIMAL THIN THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS) FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL) URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 3112 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1354 ANIMAL NUMBER: C92941 SEX: FEMALE DATE OF DEATH: 05/15/98 STUDY DAY OF DATE AND TIME OF NECROPSY: 05/15/98 9:05 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 209.6 GRAMS PROSECTOR: JODI THIEREN RECORDER: DEBBIE PIRKEL PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN STATUS LF ADRENAL (AD0) RT ADRENAL (AD1) BRAIN (BR) LF KIDNEY (KD0) RT KIDNEY (KD1) LIVER (LI) LUNG (LU) LF OVARY (OV0) RT OVARY (OV1) SPLEEN (SP) LF THYROID/PARA RT THYROID/PARA UTERUS (UT) (TT0) (TT1) -------- 8.4862 -------- -------- -------- -------- 4.0494 % -------- -------- -------- -------- --------------- -------- -------- NOT TAKEN WEIGHT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS NOTTAK NOTTAK NOTTAK NOTTAK NOTTAK NOTTAK NOTTAK NOTTAK 3113 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1355 ANIMAL NUMBER: C92941 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 209.6 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 9:05 PROSECTOR: JODI THIEREN RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) 3114 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1356 ANIMAL NUMBER: C92942 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/02/99 STUDY DAY OF DEATH: 348 STUDY WEEK OF DEATH: 50 TERMINAL BODY WEIGHT: 406.2 GRAMS DATE AND TIME OF NECROPSY: 04/02/99 15:14 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); DORSAL; WEEK 11; 85 X 80MM; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; DORSAL; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); VENTRAL-ABDOMINAL-RIGHT; WEEK 50; 35 X 25MM; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY SPLEEN (SP) : -LARGE; 12 X 17 X 75 MM LUNG (LU) : -RED FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE RED FOCI, PINPOINT AND UP TO 1 MM IN DIAMETER HISTOPATHOLOGY LIVER (LI) : -HEMATOPOIESIS, EXTRAMEDULLARY,MODERATE KIDNEY (KD) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -MINERALIZATION, TUBULAR,-MINIMAL -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SEVERE ADRENAL, CORTEX (AC) : -HEMATOPOIESIS, EXTRAMEDULLARY,-SLIGHT MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,-SEVERE MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,MODERATELY SEVERE LUNG (LU) : -HEMORRHAGE,-PRESENT -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL MAMMARY, FEMALE (MF) : -M-CARCINOMA,-NEOPLASM PRESENT -B-FIBROADENOMA,-NEOPLASM PRESENT 3115 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1357 ANIMAL NUMBER: C92942 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/02/99 STUDY DAY OF DEATH: 348 STUDY WEEK OF DEATH: 50 TERMINAL BODY WEIGHT: 406.2 GRAMS DATE AND TIME OF NECROPSY: 04/02/99 15:14 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT INGUINAL REGION: SINGLE MASS, 23 X 28 X 40 MM, FIRM, TAN, WHITE AND LOBULATED; SAME ON CUT SURFACE (CAP 20); REGIONAL LN (INGUINAL) NOT IDENTIFIED -MASS #2; LEFT INGUINAL REGION: SINGLE MASS, 50 X 60 X 70 MM, FIRM, RED, DARK RED, TAN AND LOBULATED; SAME ON CUT SURFACE; OVERLYING SKIN HAS A 35 X 40 MM DARK BROWN, DRY CRUSTED AREA; COLLECTED INTACT WITH MASS; REGIONAL LN (INGUINAL) NOT IDENTIFIED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN MAMMARY, FEMALE (MF) : >NOTE:>MASS #1 IS THE FIBROADENOMA CAUSE OF DEATH (CD) : -NEOPLASM, MAMMARY GLAND,-PRESENT 3116 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1358 ANIMAL NUMBER: C92942 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/02/99 STUDY DAY OF DEATH: 348 STUDY WEEK OF DEATH: 50 TERMINAL BODY WEIGHT: 406.2 GRAMS DATE AND TIME OF NECROPSY: 04/02/99 15:14 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP),JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM(DP), CAVITY, THORACIC (TA),LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG),STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 3117 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1359 ANIMAL NUMBER: C92943 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/25/00 STUDY DAY OF DEATH: 677 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 318.7 GRAMS DATE AND TIME OF NECROPSY: 02/25/00 8:38 PROSECTOR: NANCY DIEDRICH RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; HEAD TILT; LEFT; VERIFIED AT NECROPSY: NOT APPLICABLE >MASS(ES); INGUINAL REGION-RIGHT; WEEK 92; 20 X 20MM; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -CYST(S); VISCERAL SURFACE OF RIGHT MEDIAN LOBE: SINGLE CLEAR FLUID FILLED CYST, 1 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION ADRENAL, CORTEX (AC) : -LARGE; RIGHT: 8 MM IN DIAMETER MM (LEFT IS 5 X 3 X 2 MM) -RED FOCUS(I)/AREAS(I); RIGHT: SINGLE DARK RED AREA, 7 X 5 MM LN, MESENTERIC (MS) : -DIFFUSELY DARK; GREEN HISTOPATHOLOGY BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, PERIPORTAL,-SLIGHT -CYST, BILIARY,-PRESENT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, PELVIC,-SLIGHT -MINERALIZATION, TUBULAR,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : -HYPERTROPHY, CORTICAL CELL,-MODERATE -DEGENERATION, CYSTIC / HEMORRHAGIC,MODERATELY SEVERE -PIGMENT,-MINIMAL LN, MESENTERIC (MS) : -INFILTRATE, MACROPHAGE, PIGMENTED,SLIGHT 3118 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1360 ANIMAL NUMBER: C92943 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/25/00 STUDY DAY OF DEATH: 677 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 318.7 GRAMS DATE AND TIME OF NECROPSY: 02/25/00 8:38 PROSECTOR: NANCY DIEDRICH RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY OVARY (OV) : -CYST(S); RIGHT: SINGLE CLEAR FLUID FILLED CYST, 3 MM IN DIAMETER THYROID (TY) : -LARGE; RIGHT: 9 X 4 X 3 MM (LEFT IS 5 X 3 X 2 MM) -DIFFUSELY LIGHT; BILATERAL: TAN PITUITARY (PI) : -MASS(ES); 15 X 10 X 10 MM, RED, DARK RED, BROWN, TAN, AND SOFT MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT PERINEAL REGION: SINGLE MASS, 30 X 25 X 20 MM, RED, TAN, LOBULATED, AND FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (INGUINAL) NORMAL (CAP 22) -THICKENED; ALL REGIONS DIFFUSE: TAN AND FIRM (CAP 21) UTERUS (UT) : -THICKENED WALL; BILATERAL, DIFFUSE CERVIX (CV) : -LARGE; 10 MM IN DIAMETER OVARY (OV) : -CYST,-PRESENT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY) MODERATE THYROID (TY) : >UNREMARKABLE PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT -B-FIBROADENOMA,- 2 NEOPLASMS PRESENT >NOTE:>FIBROADENOMA ALSO ON SLIDE #21 UTERUS (UT) : >UNREMARKABLE CERVIX (CV) : -HYPERPLASIA, MUCINOUS,-MODERATE VAGINA (VA) : -HYPERPLASIA, MUCINOUS,-MODERATE 3119 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1361 ANIMAL NUMBER: C92943 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/25/00 STUDY DAY OF DEATH: 677 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 318.7 GRAMS DATE AND TIME OF NECROPSY: 02/25/00 8:38 PROSECTOR: NANCY DIEDRICH RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY TAIL (TI) : -CRUSTED AREA(S); PROXIMAL ONE-THIRD OF TAIL: MULTIPLE BROWN DRY CRUSTED AREAS, UP TO 3 MM IN DIAMETER; COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT TAIL (TI) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE -ACANTHOSIS / HYPERKERATOSIS,-MODERATE 3120 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1362 ANIMAL NUMBER: C92943 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/25/00 STUDY DAY OF DEATH: 677 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 318.7 GRAMS DATE AND TIME OF NECROPSY: 02/25/00 8:38 PROSECTOR: NANCY DIEDRICH RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), HEMATO NEOPLASIA (HN), LN, INGUINAL (IN) 3121 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1363 ANIMAL NUMBER: C92944 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 346.5 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 11:16 PROSECTOR: TROY ELLENBECKER RECORDER: KIMBERLY DURLAND POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) LF ADRENAL (AD0) RT ADRENAL (AD1) BRAIN (BR) LF KIDNEY (KD0) RT KIDNEY (KD1) LIVER (LI) LUNG (LU) LF OVARY (OV0) RT OVARY (OV1) SPLEEN (SP) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) UTERUS (UT) .0428 .0403 2.2158 1.2156 1.2941 10.8619 1.4298 .0969 .1016 .6829 .0137 .0135 .7094 .0124 % .0116 % .6395 % .3508 % .3735 % 3.1347 % .4126 % .0280 % .0293 % .1971 % .0040 % .0039 % .2047 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0193 .0182 1.0000 .5486 .5840 4.9020 .6453 .0437 .0459 .3082 .0062 .0061 .3202 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 3122 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1364 ANIMAL NUMBER: C92944 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 346.5 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 11:16 PROSECTOR: TROY ELLENBECKER RECORDER: KIMBERLY DURLAND POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV) 3123 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1365 ANIMAL NUMBER: C92945 SEX: FEMALE DATE OF DEATH: 05/15/98 STUDY DAY OF DATE AND TIME OF NECROPSY: 05/15/98 9:06 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 191.0 GRAMS PROSECTOR: LYNN HORNER RECORDER: DEBBIE PIRKEL PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN STATUS LF ADRENAL (AD0) RT ADRENAL (AD1) BRAIN (BR) LF KIDNEY (KD0) RT KIDNEY (KD1) LIVER (LI) LUNG (LU) LF OVARY (OV0) RT OVARY (OV1) SPLEEN (SP) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) UTERUS (UT) ------------------------------------ 6.9870 -------------------------------------------------- ------------------------------------ 3.6589 % -------------------------------------------------- -------------------------------------------------------------------------------------------- NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN WEIGHT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS 3124 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1366 ANIMAL NUMBER: C92945 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 191.0 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 9:06 PROSECTOR: LYNN HORNER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) 3125 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1367 ANIMAL NUMBER: C92946 SEX: FEMALE DATE OF DEATH: 04/20/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/20/00 8:10 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 565.2 GRAMS PROSECTOR: TONY HANFELD RECORDER: JERALD W. JOHNSON PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); AXILLARY REGION-RIGHT; WEEK 105; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY PITUITARY (PI) : -MASS(ES); 6 X 5 X 4 MM, DARK RED, TAN AND SOFT MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT AXILLARY REGION: SINGLE MASS, 25 X 15 X 12 MM, RED, DARK RED, TAN AND FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (AXILLARY) NORMAL (CAP 22) HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MODERATE KIDNEY (KD) : -MINERALIZATION, PELVIC,-SLIGHT PANCREAS (PA) : -ATROPHY, ACINAR,-MINIMAL -INFILTRATION, FAT, INTERSTITIAL,SLIGHT THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : -HYPERPLASIA,-SLIGHT MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT -HYPERPLASIA, GLANDULAR,-SLIGHT -B-ADENOMA,-NEOPLASM PRESENT 3126 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1368 ANIMAL NUMBER: C92946 SEX: FEMALE DATE OF DEATH: 04/20/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/20/00 8:10 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 565.2 GRAMS PROSECTOR: TONY HANFELD RECORDER: JERALD W. JOHNSON PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -THICKENED; ALL REGIONS, DIFFUSE: COLLECTED ON ROUTINE SECTION UTERUS (UT) : -CYST(S); LEFT HORN, WITHIN LUMEN: SINGLE CLEAR FLUID FILLED CYST, 5 X 3 X 3 MM GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN URINARY BLADDER (UB) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL UTERUS (UT) : -HYPERPLASIA, CYSTIC, ENDOMETRIAL,MODERATELY SEVERE CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, AXILLARY (AX) : -HYPERPLASIA, LYMPHOPLASMACYTIC,MODERATELY SEVERE 3127 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1369 ANIMAL NUMBER: C92946 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 565.2 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 8:10 PROSECTOR: TONY HANFELD RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PARATHYROID (PT) 3128 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1370 ANIMAL NUMBER: C92947 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/04/00 STUDY DAY OF DEATH: 656 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: 349.0 GRAMS DATE AND TIME OF NECROPSY: 02/04/00 9:45 PROSECTOR: TONY HANFELD RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; THIN; VERIFIED AT NECROPSY: NOT OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL -HYPERPLASIA, TRANSITIONAL CELL,-SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SLIGHT PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-SLIGHT ADRENAL, CORTEX (AC) : -HYPERTROPHY, CORTICAL CELL,-MODERATE -DEGENERATION, CYSTIC / HEMORRHAGIC,MODERATE -PIGMENT,-SLIGHT MARROW, FEMUR (FM) : -HYPOCELLULAR, MARROW,-MODERATELY SEVERE 3129 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1371 ANIMAL NUMBER: C92947 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/04/00 STUDY DAY OF DEATH: 656 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: 349.0 GRAMS DATE AND TIME OF NECROPSY: 02/04/00 9:45 PROSECTOR: TONY HANFELD RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 7 X 6 X 5 MM, RED, DARK RED, TAN, AND SOFT VAGINA (VA) : -SEMIFLUID MATERIAL; WITHIN LUMEN: LIGHT GREEN, MUCOID SEMIFLUID GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL UPPER AND LOWER INCISORS -DISCHARGE-OCULAR; BILATERAL: DRY, RED MATERIAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),SLIGHT LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -HYPERPLASIA, GLANDULAR,-MINIMAL CERVIX (CV) : -HYPERPLASIA, MUCINOUS,-MODERATE VAGINA (VA) : -LUMEN, EXUDATE,-PRESENT -HYPERPLASIA, MUCINOUS,-MODERATE CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 3130 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1372 ANIMAL NUMBER: C92947 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/04/00 STUDY DAY OF DEATH: 656 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: 349.0 GRAMS DATE AND TIME OF NECROPSY: 02/04/00 9:45 PROSECTOR: TONY HANFELD RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), HEMATO NEOPLASIA (HN) 3131 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1373 ANIMAL NUMBER: C92948 SEX: FEMALE DATE OF DEATH: 03/10/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 03/10/00 10:56 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 691 STUDY WEEK OF DEATH: 99 TERMINAL BODY WEIGHT: 400.9 GRAMS PROSECTOR: DAVID SCHUETTE RECORDER: TONE EVERTS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); VENTRAL-CERVICAL; WEEK 96; 10 X 10MM; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; ROUGH HAIR COAT; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; THIN; VERIFIED AT NECROPSY: NOT OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY LIVER (LI) : -MOTTLED; ALL LOBES: DARK RED, AND TAN -RED FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE DARK RED AREAS, UP TO 4 MM IN DIAMETER; COLLECTED ON ROUTINE SECTIONS SPLEEN (SP) : -LIGHT FOCUS(I)/AREA(S); ALONG MARGIN: SINGLE TAN AREA, 6 MM IN DIAMETER ADRENAL, CORTEX (AC) : -LARGE; LEFT: 23 X 20 X 17 MM (RIGHT IS 4 X 3 X 2 MM) HISTOPATHOLOGY LIVER (LI) : -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -CONGESTION,-PRESENT -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MODERATE -MINERALIZATION, PELVIC,-SLIGHT -HYPERPLASIA, TRANSITIONAL CELL,-SLIGHT SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE PANCREAS (PA) : -INFILTRATION, FAT, INTERSTITIAL,MODERATE ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC / HEMORRHAGIC,SEVERE -PIGMENT,-SLIGHT -B-ADENOMA,-NEOPLASM PRESENT -DEGENERATION / NECROSIS, UNILATERAL,MODERATE 3132 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1374 ANIMAL NUMBER: C92948 SEX: FEMALE DATE OF DEATH: 03/10/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 03/10/00 10:56 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 691 STUDY WEEK OF DEATH: 99 TERMINAL BODY WEIGHT: 400.9 GRAMS PROSECTOR: DAVID SCHUETTE RECORDER: TONE EVERTS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY THYMUS (TH) : -MISSING/NOT IDENTIFIED; NOT POSITIVELY IDENTIFIED; SUBMITTED WITH THICKENED ADIPOSE TISSUE (CAP 23) MAMMARY, FEMALE (MF) : -MASS(ES); LEFT VENTRAL CERVICAL REGION: SINGLE MASS, 30 X 17 X 10 MM, TAN AND SOFT; SAME ON CUT SURFACE (CAP 24); REGIONAL LN (SEE MANDIBULAR) -THICKENED; ALL REGIONS DIFFUSE: TAN; COLLECTED ON ROUTINE SECTION ADRENAL, MEDULLA (MA) : -UNILATERALLY EXAMINED,-PRESENT THYMUS (TH) : >TISSUE MISSING LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL -EMBOLISM, ADIPOSE TISSUE,-MODERATELY SEVERE HEART (HT) : -CARDIOMYOPATHY,-MINIMAL MUSCLE, SKELETAL (SM) : -ATROPHY,-SLIGHT THYROID (TY) : -HYPERPLASIA, C-CELL,-SLIGHT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT -B-FIBROADENOMA,-NEOPLASM PRESENT CERVIX (CV) : -HYPERPLASIA, MUCINOUS,-MINIMAL 3133 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1375 ANIMAL NUMBER: C92948 SEX: FEMALE DATE OF DEATH: 03/10/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 03/10/00 10:56 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 691 STUDY WEEK OF DEATH: 99 TERMINAL BODY WEIGHT: 400.9 GRAMS PROSECTOR: DAVID SCHUETTE RECORDER: TONE EVERTS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY LN, AXILLARY (AX) : -LARGE; BILATERAL: EACH IS UP TO 17 X 10 X 4 MM (CAP 21) LN, MANDIBULAR (MN) : -LARGE; BILATERAL, DEEP CERVICAL: EACH IS UP TO 15 X 7 X 4 MM (CAP 22) ADIPOSE TISSUE (AT) : -THICKENED; SURROUNDING THYMUS: TAN AND SOFT (CAP 23) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN VAGINA (VA) : -LUMEN, EXUDATE,-PRESENT -HYPERPLASIA, MUCINOUS,-SLIGHT CAUSE OF DEATH (CD) : -EMBOLISM, PULMONARY,-PRESENT LN, AXILLARY (AX) : -HYPERPLASIA, LYMPHOPLASMACYTIC,-SLIGHT LN, MANDIBULAR (MN) : >TISSUE MISSING ADIPOSE TISSUE (AT) : >UNREMARKABLE 3134 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1376 ANIMAL NUMBER: C92948 SEX: FEMALE DATE OF DEATH: 03/10/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 03/10/00 10:56 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 691 STUDY WEEK OF DEATH: 99 TERMINAL BODY WEIGHT: 400.9 GRAMS PROSECTOR: DAVID SCHUETTE RECORDER: TONE EVERTS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MEs /pANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), HEMATO NEOPLASIA (HN) 3135 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1377 ANIMAL NUMBER: C92949 SEX: FEMALE DATE OF DEATH: 12/04/98 STUDY DAY OF DATE AND TIME OF NECROPSY: 12/04/98 11:46 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 229 STUDY WEEK OF DEATH: 33 TERMINAL BODY WEIGHT: 471.1 GRAMS PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); VENTRAL-CERVICAL-LEFT; WEEK 3; 80 X 60MM; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; VENTRALCERVICAL-LEFT; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY HISTOPATHOLOGY LIVER (LI) : -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, PERIPORTAL,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MINIMAL -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SLIGHT MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,-MODERATE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATE PITUITARY (PI) : -HYPERPLASIA,-MINIMAL MAMMARY, FEMALE (MF) : -B-FIBROADENOMA,-NEOPLASM PRESENT 3136 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1378 ANIMAL NUMBER: C92949 SEX: FEMALE DATE OF DEATH: 12/04/98 STUDY DAY OF DATE AND TIME OF NECROPSY: 12/04/98 11:46 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 229 STUDY WEEK OF DEATH: 33 TERMINAL BODY WEIGHT: 471.1 GRAMS PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS(ES); VENTRAL CERVICAL REGION, EXTENDING TO BILATERAL SHOULDER REGIONS: SINGLE MASS, 90 X 55 X 45 MM (104.9 GRAMS), TAN, RED, FIRM, LOBULATED AND VASCULAR; SAME ON CUT SURFACE; SKIN OVERLYING MASS HAS MULTIPLE MOIST, DARK BROWN ABRADED AREAS, UP TO 15 MM IN DIAMETER; COLLECTED INTACT; REGIONAL LN (MANDIBULAR) NORMAL (CAP 20) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN CAUSE OF DEATH (CD) : -NEOPLASM, MAMMARY GLAND,-PRESENT LN, MANDIBULAR (MN) : -HYPERPLASIA, LYMPHOPLASMACYTIC,-SLIGHT 3137 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1379 ANIMAL NUMBER: C92949 SEX: FEMALE DATE OF DEATH: 12/04/98 STUDY DAY OF DATE AND TIME OF NECROPSY: 12/04/98 11:46 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 229 STUDY WEEK OF DEATH: 33 TERMINAL BODY WEIGHT: 471.1 GRAMS PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 3138 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1380 ANIMAL NUMBER: C92950 SEX: FEMALE DATE OF DEATH: 05/15/98 STUDY DAY OF DATE AND TIME OF NECROPSY: 05/15/98 10:41 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 191.7 GRAMS PROSECTOR: JODI THIEREN RECORDER: DEBBIE PIRKEL PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN STATUS LF ADRENAL (AD0) RT ADRENAL (AD1) BRAIN (BR) LF KIDNEY (KD0) RT KIDNEY (KD1) LIVER (LI) LUNG (LU) LF OVARY (OV0) RT OVARY (OV1) SPLEEN (SP) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) UTERUS (UT) ------------------------------------ 7.1161 -------------------------------------------------- ------------------------------------ 3.7119 % -------------------------------------------------- -------------------------------------------------------------------------------------------- NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN WEIGHT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS 3139 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1381 ANIMAL NUMBER: C92950 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 191.7 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 10:41 PROSECTOR: JODI THIEREN RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) 3140 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1382 ANIMAL NUMBER: C92951 SEX: FEMALE DATE OF DEATH: 04/03/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/03/00 14:03 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 715 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 409.8 GRAMS PROSECTOR: JOHN S. HALFORD RECORDER: JARED FISHEL PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); AXILLARY REGION-RIGHT; WEEK 64; 50 X 50MM; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; AXILLARY REGION-RIGHT; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); VENTRAL-ABDOMINAL-RIGHT; WEEK 80; 60 X 45MM; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) SPLEEN (SP) : -LARGE; 51 X 18 X 11 MM HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -HEMATOPOIESIS, EXTRAMEDULLARY,-SLIGHT -HYPERPLASIA, BILE DUCT,-MINIMAL -FIBROSIS, PERIBILIARY,-SLIGHT -HYPERTROPHY, HEPATOCELLULAR, PERIPORTAL,-MODERATE KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SEVERE PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-MODERATE ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL -HYPERTROPHY, CORTICAL CELL,-MINIMAL -DEGENERATION, CYSTIC / HEMORRHAGIC,MINIMAL 3141 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1383 ANIMAL NUMBER: C92951 SEX: FEMALE DATE OF DEATH: 04/03/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/03/00 14:03 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 715 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 409.8 GRAMS PROSECTOR: JOHN S. HALFORD RECORDER: JARED FISHEL PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 5 X 4 X 3 MM, RED, GRAY, TAN AND SOFT ADRENAL, CORTEX (AC) : -PIGMENT,-MINIMAL MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,MODERATELY SEVERE MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,MODERATELY SEVERE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),SLIGHT LUNG (LU) : -INFILTRATE, LYMPHOHISTIOCYTIC,-SLIGHT HEART (HT) : -CARDIOMYOPATHY,-MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT DUODENUM (DU) : -B-LEIOMYOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -M-CARCINOMA,-NEOPLASM PRESENT -CYST / GALACTOCELE,-PRESENT -B-FIBROADENOMA,-NEOPLASM PRESENT -HYPERPLASIA, GLANDULAR,-SLIGHT >NOTE:>THE FIBROADENOMA IS MASS #1 3142 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1384 ANIMAL NUMBER: C92951 SEX: FEMALE DATE OF DEATH: 04/03/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/03/00 14:03 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 715 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 409.8 GRAMS PROSECTOR: JOHN S. HALFORD RECORDER: JARED FISHEL PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT INGUINAL REGION: SINGLE MASS, 68 X 58 X 25 MM, RED, DARK RED, GRAY, TAN, VASCULAR, LOBULATED AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (INGUINAL) NORMAL (CAP 21) -MASS #2; RIGHT AXILLARY REGION: SINGLE MASS, 65 X 41 X 38 MM, RED, DARK RED, GRAY, TAN, LOBULATED AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (SEE AXILLARY); OVERLYING SKIN HAS A SINGLE DRY RED AND TAN ULCERATED AREA, 50 X 30 X 17 MM; COLLECTED INTACT WITH MASS -CYST(S); ALL REGIONS, DIFFUSE: MULTIPLE TAN AND WHITE CREAMY SEMIFLUID FILLED CYSTS, UP TO 2 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION CERVIX (CV) : -MASS(ES); WITHIN LUMEN, EXTENDING INTO BIFURCATION OF UTERINE HORNS: SINGLE POLYPOID MASS, 8 X 3 X 3 MM, RED, GRAY AND SOFT; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (LUMBAR) NORMAL (CAP 23) UTERUS (UT) : -HYPERPLASIA, CYSTIC, ENDOMETRIAL,MODERATE CERVIX (CV) : -B-POLYP, STROMAL,-NEOPLASM PRESENT 3143 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1385 ANIMAL NUMBER: C92951 SEX: FEMALE DATE OF DEATH: 04/03/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/03/00 14:03 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 715 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 409.8 GRAMS PROSECTOR: JOHN S. HALFORD RECORDER: JARED FISHEL PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY LN, AXILLARY (AX) : -LARGE; RIGHT: UP TO 15 X 7 X 4 MM (LEFT IS UP TO 4 X 3 X 1 MM) (CAP 22) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL: UPPER AND LOWER INCISORS -ANIMAL THIN CAUSE OF DEATH (CD) : -NEOPLASM, MAMMARY GLAND,-PRESENT LN, AXILLARY (AX) : -HYPERPLASIA, LYMPHOPLASMACYTIC,MODERATE LN, INGUINAL (IN) : >TISSUE MISSING >NOTE:>TISSUE IS PART OF MAMMARY TUMOR MASS LN, LUMBAR/ILIAC (LM) : -HYPERPLASIA, LYMPHOPLASMACYTIC,MODERATE 3144 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1386 ANIMAL NUMBER: C92951 SEX: FEMALE DATE OF DEATH: 04/03/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/03/00 14:03 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 715 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 409.8 GRAMS PROSECTOR: JOHN S. HALFORD RECORDER: JARED FISHEL PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), VAGINA (VA), LN, MEDIASTINAL (ML), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), VAGINA (VA), HEMATO NEOPLASIA (HN) 3145 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1387 ANIMAL NUMBER: C92952 SEX: FEMALE DATE OF DEATH: 07/19/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 07/19/99 11:09 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 456 STUDY WEEK OF DEATH: 66 TERMINAL BODY WEIGHT: 300.2 GRAMS PROSECTOR: LYNN HORNER RECORDER: STEVE VAN ADESTINE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); AXILLARY REGION-LEFT; WEEK 63; 20 X 20MM; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >EYES; RED DISCHARGE; EYES; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) HISTOPATHOLOGY BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL.-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL -FIBROSIS, PERIBILIARY,-MINIMAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-SLIGHT -INFLAMMATION, CHRONIC-ACTIVE, PELVIC,SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SLIGHT ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT -HYPERTROPHY, CORTICAL CELL,-MINIMAL -PIGMENT,-MINIMAL 3146 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1388 ANIMAL NUMBER: C92952 SEX: FEMALE DATE OF DEATH: 07/19/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 07/19/99 11:09 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 456 STUDY WEEK OF DEATH: 66 TERMINAL BODY WEIGHT: 300.2 GRAMS PROSECTOR: LYNN HORNER RECORDER: STEVE VAN ADESTINE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 13 X 9 X 6 MM, DARK RED, TAN, AND SOFT MAMMARY, FEMALE (MF) : -MASS(ES); LEFT AXILLARY REGION: SINGLE MASS, 17 X 15 X 9 MM, DARK RED TAN, AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (AXILLARY) NORMAL (CAP 20) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DARK RED, DRY, CRUSTED MATERIAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY) MINIMAL HEART (HT) : -CARDIOMYOPATHY,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -M-CARCINOMA,-NEOPLASM PRESENT -CYST / GALACTOCELE,-PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 3147 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1389 ANIMAL NUMBER: C92952 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/19/99 STUDY DAY OF DEATH: 456 STUDY WEEK OF DEATH: 66 TERMINAL BODY WEIGHT: 300.2 GRAMS DATE AND TIME OF NECROPSY: 07/19/99 11:09 PROSECTOR: LYNN HORNER RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN), LN, AXILLARY (AX) 3148 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1390 ANIMAL NUMBER: C92953 SEX: FEMALE DATE OF DEATH: 04/21/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/21/00 7:23 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 439.9 GRAMS PROSECTOR: JOHN S. HALFORD RECORDER: LAURIE J. SCHULLER PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY PITUITARY (PI) : -DARK FOCUS(I)/AREA(S); LEFT POSTERIOR ASPECT: SINGLE BROWN FOCUS, 2 MM IN DIAMETER LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL: (CAP 22) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN DARK RED HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PITUITARY (PI) : -HYPERPLASIA,-MINIMAL -ANGIECTASIS,-PRESENT MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT -HYPERPLASIA, GLANDULAR,-MODERATE URINARY BLADDER (UB) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MANDIBULAR (MN) : -HEMORRHAGE,-PRESENT 3149 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1391 ANIMAL NUMBER: C92953 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 439.9 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 7:23 PROSECTOR: JOHN S. HALFORD RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: KIDNEY (KD), PANCREAS (PA), LUNG (LU), PARATHYROID (PT) 3150 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1392 ANIMAL NUMBER: C92954 SEX: FEMALE DATE OF DEATH: 03/23/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 03/23/00 7:54 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 704 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: 422.4 GRAMS PROSECTOR: JILL PAUS RECORDER: JILL PAUS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); VENTRAL-CERVICAL-RIGHT; WEEK 77; 40 X 50MM; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; NO FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; RED HAIR COAT; HEADENTIRE; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; YELLOW HAIR COAT; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) KIDNEY (KD) : -DIFFUSELY LIGHT; BILATERAL: BROWN ADRENAL, CORTEX (AC) : -LARGE; LEFT: 15 X 10 X 7 MM (RIGHT IS 4 MM IN DIAMETER) -MOTTLED; LEFT: RED, DARK RED AND TAN HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-MODERATE -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-SLIGHT -HYPERPLASIA, BILE DUCT,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, PELVIC,-SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-SLIGHT -INFILTRATION, FAT, INTERSTITIAL,SLIGHT ADRENAL, CORTEX (AC) : >UNREMARKABLE 3151 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1393 ANIMAL NUMBER: C92954 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/23/00 STUDY DAY OF DEATH: 704 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: 422.4 GRAMS DATE AND TIME OF NECROPSY: 03/23/00 7:54 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PARATHYROID (PT) : -LARGE; RIGHT: 2 X 1 X 1 MM (LEFT IS LESS THAN 1 MM) PITUITARY (PI) : -MASS(ES); 8 X 6 X 6 MM, RED , DARK RED, TAN AND SOFT STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA: MULTIPLE RED, TAN ERODED AREAS, UP TO 4 X 2 MM ADRENAL, MEDULLA (MA) : -M-MALIGNANT PHEOCHROMOCYTOMA,-NEOPLASM PRESENT MARROW, FEMUR (FM) : -HYPOCELLULAR, MARROW,-MODERATELY SEVERE MARROW, STERNUM (SE) : -HYPERPLASIA, ERYTHROID, MARROW,MODERATE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATE HEART (HT) : -CARDIOMYOPATHY,-SLIGHT PARATHYROID (PT) : -B-ADENOMA,-NEOPLASM PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, NONGL (SU) : -ACANTHOSIS / HYPERKERATOSIS,-SLIGHT -ULCERATION,-SLIGHT -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE MAMMARY, FEMALE (MF) : -B-FIBROADENOMA,-NEOPLASM PRESENT 3152 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1394 ANIMAL NUMBER: C92954 SEX: FEMALE DATE OF DEATH: 03/23/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 03/23/00 7:54 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 704 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: 422.4 GRAMS PROSECTOR: JILL PAUS RECORDER: JILL PAUS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS(ES); VENTRAL CERVICAL REGION: SINGLE MASS, 50 X 45 X 15 MM, RED, DARK RED, TAN, FIRM AND LOBULATED; SAME ON CUT SURFACE; SOME SECTIONS EXUDE A TAN CREAMY SEMIFLUID; COLLECTED; REGIONAL LN (MANDIBULAR) NORMAL (CAP 21) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -STAINS-PERINEUM/PERIANAL; YELLOW -DISCHARGE-NASAL; RED, DRY MATERIAL UTERUS (UT) : -HYPERPLASIA, CYSTIC, ENDOMETRIAL,MINIMAL VAGINA (VA) : -LUMEN, EXUDATE,-PRESENT -HYPERPLASIA, MUCINOUS,-SLIGHT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT LN, MANDIBULAR (MN) : -HYPERPLASIA, LYMPHOPLASMACYTIC,-SLIGHT 3153 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1395 ANIMAL NUMBER: C92954 SEX: FEMALE DATE OF DEATH: 03/23/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 03/23/00 7:54 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 704 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: 422.4 GRAMS PROSECTOR: JILL PAUS RECORDER: JILL PAUS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), HEMATO NEOPLASIA (HN) 3154 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1396 ANIMAL NUMBER: C92955 SEX: FEMALE DATE OF DEATH: 01/17/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 01/17/00 14:21 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 638 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: 331.9 GRAMS PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); INGUINAL REGION-LEFT; WEEK 75; 40 X 40MM; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; DORSALCERVICAL; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); INGUINAL REGION-RIGHT; WEEK 83; 20 X 20MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); VENTRAL-ABDOMINAL-RIGHT; WEEK 84; 25 X 25MM; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; YELLOW HAIR COAT; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; ATAXIC; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL -HYPERPLASIA, BILE DUCT,-SLIGHT -FIBROSIS, PERIBILIARY,-SLIGHT -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, PELVIC,-MINIMAL -HYPERPLASIA, TRANSITIONAL CELL,MODERATE -INFLAMMATION, SUPPURATIVE, PELVIC,SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATE -HYPERTROPHY, CORTICAL CELL,-MINIMAL BONE, FEMUR (FE) : -HYPERPLASIA, CARTILAGE,-MODERATE 3155 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1397 ANIMAL NUMBER: C92955 SEX: FEMALE DATE OF DEATH: 01/17/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 01/17/00 14:21 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 638 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: 331.9 GRAMS PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 11 X 10 X 7 MM, RED, TAN, VASCULAR AND SOFT SKIN (SK) : -CRUSTED AREA(S); DORSAL CERVICAL REGION: SINGLE TAN DRY CRUSTED AREA, 2 MM IN DIAMETER (CAP 21) MAMMARY, FEMALE (MF) : -MASS(ES); LEFT INGUINAL REGION: SINGLE MASS, 50 X 32 X 15 MM, TAN AND FIRM; SAME ON CUT SURFACE (CAP 22); REGIONAL LN (INGUINAL) NOT IDENTIFIED -MASS #2; RIGHT INGUINAL REGION: SINGLE MASS, 33 X 24 X 15 MM, TAN AND FIRM; SAME ON CUT SURFACE (CAP 23); REGIONAL LN (INGUINAL) NOT IDENTIFIED -MASS #3; RIGHT INGUINAL REGION, CRANIAL TO MASS 2: SINGLE MASS, 35 X 25 X 20 MM, TAN AND FIRM; SAME ON CUT SURFACE; (CAP 24); REGIONAL LN (INGUINAL) NOT IDENTIFIED THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATELY SEVERE THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT SKIN (SK) : -ACANTHOSIS/HYPERKERATOSIS,-SLIGHT -INFLAMMATION, CHRONIC,-MODERATE MAMMARY, FEMALE (MF) : -M-CARCINOMA,-NEOPLASM PRESENT -B-FIBROADENOMA,- 2 NEOPLASMS PRESENT -HYPERPLASIA, GLANDULAR,-MODERATELY SEVERE >NOTE:>MASS #3 IS THE CARCINOMA URINARY BLADDER (UB) : -INFILTRATE, LYMPHOHISTIOCYTIC,-SLIGHT 3156 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1398 ANIMAL NUMBER: C92955 SEX: FEMALE DATE OF DEATH: 01/17/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 01/17/00 14:21 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 638 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: 331.9 GRAMS PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY UTERUS (UT) : -THICKENED WALL; -LARGE, DIFFUSE; IN DIAMETER BILATERAL, DIFFUSE BILATERAL: UP TO 6 MM GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN -STAINS-PERINEUM/PERIANAL; MATERIAL DRY BROWN URINARY BLADDER (UB) : -HYPERPLASIA, TRANSITIONAL CELL,-SLIGHT UTERUS (UT) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT -HYPERPLASIA, CYSTIC, ENDOMETRIAL,SEVERE CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 3157 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1399 ANIMAL NUMBER: C92955 SEX: FEMALE DATE OF DEATH: 01/17/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 01/17/00 14:21 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 638 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: 331.9 GRAMS PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUm /s KULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) (SG), 3158 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1400 ANIMAL NUMBER: C92956 SEX: FEMALE DATE OF DEATH: 03/28/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 03/28/00 11:50 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 709 STUDY WEEK OF DEATH: 102 TERMINAL BODY WEIGHT: 558.7 GRAMS PROSECTOR: JERALD W. JOHNSON RECORDER: JERALD W. JOHNSON PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); INGUINAL REGION-LEFT; WEEK 82; 40 X 50MM; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; BLUE SKIN; VENTRALABDOMINAL-LEFT; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; SORE/SCAB; VENTRALABDOMINAL-LEFT; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -RED FOCUS(I)/AREA(S); LEFT LATERAL LOBE: MULTIPLE DARK RED AREAS, UP TO 3 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION KIDNEY (KD) : -DIFFUSELY DARK; BILATERAL: DARK BROWN SPLEEN (SP) : -LARGE; 65 X 15 X 13 MM -ADHESION(S); PARIETAL SURFACE OF CAUDAL END TO ADIPOSE TISSUE: MULTIPLE GRAY FIBROUS ADHESIONS; COLLECTED INTACT -MOTTLED; TAN, RED AND DARK RED HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-SLIGHT -HEMATOPOIESIS, EXTRAMEDULLARY,MODERATE -HYPERPLASIA, BILE DUCT,-SLIGHT -FIBROSIS, PERIBILIARY,-SLIGHT -VACUOLATION, HEPATOCELLULAR,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, PELVIC,-SLIGHT SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SEVERE -HEMORRHAGE,-PRESENT -NECROSIS,-MODERATE PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL 3159 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1401 ANIMAL NUMBER: C92956 SEX: FEMALE DATE OF DEATH: 03/28/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 03/28/00 11:50 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 709 STUDY WEEK OF DEATH: 102 TERMINAL BODY WEIGHT: 558.7 GRAMS PROSECTOR: JERALD W. JOHNSON RECORDER: JERALD W. JOHNSON PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY ADRENAL, CORTEX (AC) : -LARGE; BILATERAL: LEFT IS 8 X 5 X 5 MM, RIGHT IS 5 X 4 X 4 MM -MOTTLED; BILATERAL: DARK RED AND BROWN OVARY (OV) : -CYST(S); LEFT: SINGLE CLEAR FLUID FILLED CYST, 3 MM IN DIAMETER PANCREAS (PA) : -INFILTRATION, FAT, INTERSTITIAL,MODERATELY SEVERE -INFLAMMATION, VASCULAR,-MODERATE ADRENAL, CORTEX (AC) : -HEMATOPOIESIS, EXTRAMEDULLARY,-SLIGHT -HYPERTROPHY, CORTICAL CELL,-SEVERE -DEGENERATION, CYSTIC / HEMORRHAGIC,MODERATELY SEVERE -PIGMENT,-SLIGHT ADRENAL, MEDULLA (MA) : -HYPERPLASIA, MEDULLARY,-MINIMAL OVARY (OV) : -CYST,-PRESENT MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,-SEVERE MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,-SEVERE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),SLIGHT LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL -INFLAMMATION, VASCULAR,-SLIGHT THYROID (TY) : -HYPERPLASIA, C-CELL,-MODERATE 3160 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1402 ANIMAL NUMBER: C92956 SEX: FEMALE DATE OF DEATH: 03/28/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 03/28/00 11:50 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 709 STUDY WEEK OF DEATH: 102 TERMINAL BODY WEIGHT: 558.7 GRAMS PROSECTOR: JERALD W. JOHNSON RECORDER: JERALD W. JOHNSON PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 8 X 7 X 5 MM, TAN, RED AND SOFT MAMMARY, FEMALE (MF) : -MASS(ES); LEFT PERIANAL REGION EXTENDING TO LEFT INGUINAL REGION: SINGLE MASS, 50 X 30 X 25 MM, TAN, GRAY, RED, DARK RED, LOBULATED AND FIRM; SAME ON CUT SURFACE; REGIONAL LN (SEE INGUINAL); OVERLYING SKIN IS ULCERATED, 40 X 20 MM; COLLECTED INTACT WITH MASS -THICKENED; ALL REGIONS: DIFFUSE; COLLECTED ON ROUTINE SECTION LN, INGUINAL (IN) : -LARGE; BILATERAL: LEFT IS 6 X 5 X 4 MM, RIGHT IS 5 X 4 X 3 MM (CAP 21) LN, ANT MES/PANC (AP) : -LARGE; PANCREATIC: UP TO 2 X 2 X 1 MM (CAP 23) -DIFFUSELY RED; PANCREATIC: DARK RED (CAP 23) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -M-CARCINOMA,-NEOPLASM PRESENT -CYST / GALACTOCELE,-PRESENT -HYPERPLASIA, GLANDULAR,-SLIGHT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT LN, INGUINAL (IN) : -HYPERPLASIA, LYMPHOPLASMACYTIC,-SEVERE LN, ANT MES/PANC (AP) : -HEMORRHAGE,-PRESENT -HYPERPLASIA, LYMPHOPLASMACYTIC,MODERATE -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL 3161 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1403 ANIMAL NUMBER: C92956 SEX: FEMALE DATE OF DEATH: 03/28/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 03/28/00 11:50 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 709 STUDY WEEK OF DEATH: 102 TERMINAL BODY WEIGHT: 558.7 GRAMS PROSECTOR: JERALD W. JOHNSON RECORDER: JERALD W. JOHNSON PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LN, MESENTERIC (MS), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 3162 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1404 ANIMAL NUMBER: C92957 SEX: FEMALE DATE OF DEATH: 04/21/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/21/00 8:18 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 523.0 GRAMS PROSECTOR: STEVE VAN ADESTINE RECORDER: LAURIE J. SCHULLER PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK 63; 100 X 80MM; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LN, MESENTERIC (MS) : -LARGE; UP TO 10 X 6 X 5 MM PITUITARY (PI) : -RED FOCUS(I)/AREA(S); RIGHT POSTERIOR ASPECT: SINGLE DARK RED, SLIGHTLY RAISED AREA, 3 X 2 MM STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: MULTIPLE DARK BROWN AREAS, UP TO 4 X 1 MM HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MODERATE -HYPERPLASIA, BILE DUCT,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-MODERATE -B-ADENOMA, ISLET CELL,-NEOPLASM PRESENT LN, MESENTERIC (MS) : >UNREMARKABLE LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL PITUITARY (PI) : -HYPERPLASIA,-SLIGHT STOMACH, GL (ST) : >UNREMARKABLE 3163 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1405 ANIMAL NUMBER: C92957 SEX: FEMALE DATE OF DEATH: 04/21/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/21/00 8:18 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 523.0 GRAMS PROSECTOR: STEVE VAN ADESTINE RECORDER: LAURIE J. SCHULLER PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY COLON (CO) : -MASS(ES); 28 MM DISTAL TO CECUM, ATTACHED TO SEROSAL SURFACE: SINGLE MASS, 13 X 10 X 7 MM, TAN, YELLOW, AND FIRM; SAME ON CUT SURFACE (CAP 22); REGIONAL LN (SEE MESENTERIC) SKIN (SK) : >NOTE:>ROUTINE SECTION COLLECTED FROM RIGHT INGUINAL REGION DUE TO MASS ON THE LEFT INGUINAL REGION MAMMARY, FEMALE (MF) : -MASS(ES); LEFT INGUINAL REGION: SINGLE MASS, 120 X 95 X 40 MM (237 GRAMS), TAN, SOFT, AND LOBULATED; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (INGUINAL) NOT IDENTIFIED -CYST(S); RIGHT INGUINAL REGION: SINGLE TAN CREAMY FLUID FILLED CYST, 20 X 15 X 10 MM; COLLECTED ON ROUTINE SECTION >NOTE:>ROUTINE SECTION COLLECTED FROM RIGHT INGUINAL REGIONAL DUE TO MASS ON THE LEFT INGUINAL REGION GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN COLON (CO) : -B-LEIOMYOMA,-NEOPLASM PRESENT SKIN (SK) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE -HEMORRHAGE,-PRESENT -B-LIPOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT -B-FIBROADENOMA,-NEOPLASM PRESENT -HYPERPLASIA, GLANDULAR,-SLIGHT >NOTE:>SEE SKIN FOR DIAGNOSIS OF MASS CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 3164 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1406 ANIMAL NUMBER: C92957 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 523.0 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 8:18 PROSECTOR: STEVE VAN ADESTINE RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: THYROID (TY), PARATHYROID (PT), URINARY BLADDER (UB) 3165 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1407 ANIMAL NUMBER: C92958 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/29/99 STUDY DAY OF DEATH: 619 STUDY WEEK OF DEATH: 89 TERMINAL BODY WEIGHT: 327.2 GRAMS DATE AND TIME OF NECROPSY: 12/29/99 10:45 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; HUNCHED; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; ROUGH HAIR COAT; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; HEAD TILT; RIGHT; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; RECUMBENT; LATERAL; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) HISTOPATHOLOGY EYE (EY) : -DYSTROPHY, CORNEAL IN EARLY STAGES, CONSISTS PRIMARILY OF FOCAL MINERALIZATION,-MINIMAL BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-MODERATE -HYPERPLASIA, TRANSITIONAL CELL,-SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC / HEMORRHAGIC,SLIGHT -PIGMENT,-SLIGHT LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL 3166 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1408 ANIMAL NUMBER: C92958 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/29/99 STUDY DAY OF DEATH: 619 STUDY WEEK OF DEATH: 89 TERMINAL BODY WEIGHT: 327.2 GRAMS DATE AND TIME OF NECROPSY: 12/29/99 10:45 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 12 X 9 X 9 MM, DARK RED, GRAY AND SOFT VAGINA (VA) : -SEMIFLUID MATERIAL; WITHIN LUMEN: LIGHT GREEN AND MUCOID GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DARK RED AND DRY HEART (HT) : -CARDIOMYOPATHY,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : -EDEMA,-MODERATE -INFLAMMATION, ACUTE,-SLIGHT VAGINA (VA) : -LUMEN, EXUDATE,-PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 3167 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1409 ANIMAL NUMBER: C92958 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/29/99 STUDY DAY OF DEATH: 619 STUDY WEEK OF DEATH: 89 TERMINAL BODY WEIGHT: 327.2 GRAMS DATE AND TIME OF NECROPSY: 12/29/99 10:45 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB),UTERUS (UT), CERVIX (CV), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), MUSCLE, SKELETAL (SM), NERVE, SCIATIC TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY),PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB),UTERUS (UT), CERVIX (CV), HEMATO NEOPLASIA (HN) (SN), 3168 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1410 ANIMAL NUMBER: C92959 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/28/00 STUDY DAY OF DEATH: 680 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: 398.9 GRAMS DATE AND TIME OF NECROPSY: 02/28/00 14:41 PROSECTOR: DENNIS HOFFMAN RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; MISSING; TEETH; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; NO FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >EYES; RED DISCHARGE; EYE-RIGHT; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; BROWN HAIR COAT; VENTRAL-ABDOMINAL; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -MOTTLED; ALL LOBES : RED AND TAN; COLLECTED ON ROUTINE SECTIONS HISTOPATHOLOGY BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SLIGHT -MINERALIZATION, PELVIC,-SLIGHT -INFLAMMATION, SUPPURATIVE, PELVIC,SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-SEVERE -INFILTRATION, FAT, INTERSTITIAL,MODERATELY SEVERE ADRENAL, CORTEX (AC) : -HYPERPLASIA, CORTICAL,-SLIGHT -PIGMENT,-SLIGHT 3169 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1411 ANIMAL NUMBER: C92959 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/28/00 STUDY DAY OF DEATH: 680 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: 398.9 GRAMS DATE AND TIME OF NECROPSY: 02/28/00 14:41 PROSECTOR: DENNIS HOFFMAN RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 10 X 7 X 6 MM, SOFT, DARK RED, AND TAN STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA: MULTIPLE TAN ERODED AREAS, UP TO 1 X 2 MM VAGINA (VA) : -SEMIFLUID MATERIAL; WITHIN LUMEN: LIGHT GREEN MUCOID MATERIAL OVARY (OV) : -CYST,-PRESENT MARROW, FEMUR (FM) : -HYPOCELLULAR, MARROW,-MODERATELY SEVERE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATE HEART (HT) : -CARDIOMYOPATHY,-MINIMAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, NONGL (SU) : -ACANTHOSIS / HYPERKERATOSIS,-MODERATE -ULCERATION,-MINIMAL -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE MAMMARY, FEMALE (MF) : -HYPERPLASIA, GLANDULAR,-MODERATELY SEVERE CERVIX (CV) : -HYPERPLASIA, MUCINOUS,-MODERATE -EXUDATE, LUMINAL,-PRESENT VAGINA (VA) : -LUMEN, EXUDATE,-PRESENT -HYPERPLASIA, MUCINOUS,-MINIMAL 3170 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1412 ANIMAL NUMBER: C92959 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/28/00 STUDY DAY OF DEATH: 680 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: 398.9 GRAMS DATE AND TIME OF NECROPSY: 02/28/00 14:41 PROSECTOR: DENNIS HOFFMAN RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; LEFT UPPER INCISOR -TEETH MISSING/BROKEN/WORN; RIGHT UPPER INCISOR: MISSING -DISCHARGE-OCULAR; BILATERAL: DARK RED DRY MATERIAL -STAINS-PERINEUM/PERIANAL; MOIST BROWN MATERIAL CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 3171 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1413 ANIMAL NUMBER: C92959 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/28/00 STUDY DAY OF DEATH: 680 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: 398.9 GRAMS DATE AND TIME OF NECROPSY: 02/28/00 14:41 PROSECTOR: DENNIS HOFFMAN RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), HEMATO NEOPLASIA (HN) 3172 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1414 ANIMAL NUMBER: C92960 SEX: FEMALE DATE OF DEATH: 04/21/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/21/00 7:21 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 655.0 GRAMS PROSECTOR: TODD ACKER RECORDER: TONE EVERTS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); INGUINAL REGION-LEFT; WEEK 72; 120 X 80MM; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY ADRENAL, CORTEX (AC) : -LARGE; BILATERAL: LEFT IS 9 X 6 X 4 MM, RIGHT IS 7 X 6 X 4 MM OVARY (OV) : -CYST(S); LEFT: SINGLE CLEAR FLUID FILLED CYST, 2 MM IN DIAMETER PITUITARY (PI) : -MASS(ES); 6 X 6 X 4 MM, RED, DARK RED, TAN, AND SOFT HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-SLIGHT -HYPERTROPHY, HEPATOCELLULAR, PERIPORTAL,-MODERATE -ANGIECTASIS,-SLIGHT ADRENAL, CORTEX (AC) : -HYPERTROPHY, CORTICAL CELL,-MODERATE -DEGENERATION, CYSTIC / HEMORRHAGIC,MODERATE -PIGMENT,-SLIGHT OVARY (OV) : -CYST,-PRESENT LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL -HYPERPLASIA, TYPE II PNEUMOCYTE,MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT SKIN (SK) : >NOT REQUIRED TO BE EXAMINED FOR ANIMAL 3173 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1415 ANIMAL NUMBER: C92960 SEX: FEMALE DATE OF DEATH: 04/21/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/21/00 7:21 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 655.0 GRAMS PROSECTOR: TODD ACKER RECORDER: TONE EVERTS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY SKIN (SK) : >NOTE:>ROUTINE SECTION TAKEN FROM RIGHT INGUINAL REGION DUE TO MASS MAMMARY, FEMALE (MF) : -MASS(ES); LEFT INGUINAL REGION: SINGLE MASS, 135 X 75 X 60 MM (291.7 GRAMS), RED, DARK RED, TAN, GREY, AND FIRM, SAME ON CUT SURFACE; PORTION COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED >NOTE:>ROUTINE SECTION COLLECTED FROM RIGHT INGUINAL REGION, DUE TO MASS GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN MAMMARY, FEMALE (MF) : -B-FIBROADENOMA,-NEOPLASM PRESENT -HYPERPLASIA, GLANDULAR,-MINIMAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 3174 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1416 ANIMAL NUMBER: C92960 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 655.0 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 7:21 PROSECTOR: TODD ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: KIDNEY (KD), PANCREAS (PA), ADRENAL, MEDULLA (MA), THYROID (TY), PARATHYROID (PT), URINARY BLADDER (UB) 3175 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1417 ANIMAL NUMBER: C92961 SEX: FEMALE DATE OF DEATH: 03/10/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 03/10/00 10:56 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 691 STUDY WEEK OF DEATH: 99 TERMINAL BODY WEIGHT: 621.8 GRAMS PROSECTOR: TODD ACKER RECORDER: TONE EVERTS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); INGUINAL REGION-LEFT; WEEK 76; 120 X 70MM; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; HEAD TILT; LEFT; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; LIMITED USE; LIMB-HINDLEFT; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; BLUE SKIN; VENTRALABDOMINAL-LEFT; VERIFIED AT NECROPSY: NOT APPLICABLE >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) ADRENAL, CORTEX (AC) : -LARGE; LEFT: 5 X 5 X 3 MM (RIGHT IS 5 X 3 X 2 MM) HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -DEGENERATION, CYSTIC,-MINIMAL -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, PERIPORTAL,-SLIGHT -INFILTRATE, MACROPHAGE, PIGMENTED,SLIGHT -HEMORRHAGE,-PRESENT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, PELVIC,-MINIMAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MINIMAL ADRENAL, CORTEX (AC) : -HYPERTROPHY, CORTICAL CELL,-MODERATE -DEGENERATION, CYSTIC / HEMORRHAGIC,MODERATE -PIGMENT,-MODERATE -ATROPHY, CORTICAL,-MODERATELY SEVERE 3176 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1418 ANIMAL NUMBER: C92961 SEX: FEMALE DATE OF DEATH: 03/10/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 03/10/00 10:56 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 691 STUDY WEEK OF DEATH: 99 TERMINAL BODY WEIGHT: 621.8 GRAMS PROSECTOR: TODD ACKER RECORDER: TONE EVERTS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 14 X 9 X 8 MM, RED, DARK RED, TAN, AND SOFT STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA: TWO TAN ULCERATED AREAS, ONE IS 10 X 4 MM, THE OTHER IS 3 X 2 MM SKIN (SK) : >NOTE:>ROUTINE SECTION COLLECTED FROM RIGHT INGUINAL REGION DUE TO MASS ADRENAL, MEDULLA (MA) : -B-PHEOCHROMOCYTOMA,-NEOPLASM PRESENT MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,-MODERATE MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,-MODERATE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATE LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, NONGL (SU) : -ACANTHOSIS / HYPERKERATOSIS,-MODERATE -ULCERATION,-MODERATELY SEVERE -INFLAMMATION, CHRONIC-ACTIVE,-SEVERE MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT -INFLAMMATION, CHRONIC-ACTIVE,-SEVERE -B-ADENOMA,-NEOPLASM PRESENT -FIBROSIS,-SEVERE 3177 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1419 ANIMAL NUMBER: C92961 SEX: FEMALE DATE OF DEATH: 03/10/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 03/10/00 10:56 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 691 STUDY WEEK OF DEATH: 99 TERMINAL BODY WEIGHT: 621.8 GRAMS PROSECTOR: TODD ACKER RECORDER: TONE EVERTS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS(ES); LEFT INGUINAL REGION, EXTENDING INTO LEFT PERIANAL REGION: SINGLE MASS, 140 X 70 X 30 MM, RED, DARK RED, TAN, AND SOFT; CUT SURFACE EXUDES A LARGE AMOUNT OF A REDDISHBROWN SEMIFLUID; PORTION COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED >NOTE:>ROUTINE SECTION COLLECTED FROM RIGHT INGUINAL REGION DUE TO MASS UTERUS (UT) : -CYST(S); RIGHT PROXIMAL HORN, WITHIN LUMEN: SINGLE DARK RED FLUID FILLED CYST, 5 MM IN DIAMETER GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN GENERAL INFORMATION (XX) : >NOTE:>ANIMAL IDENTIFICATION TRANSPONDER DAMAGED AT NECROPSY UTERUS (UT) : -B-POLYP, ENDOMETRIAL, STROMAL,NEOPLASM PRESENT -HYPERPLASIA, CYSTIC, ENDOMETRIAL,SLIGHT VAGINA (VA) : -LUMEN, EXUDATE,-PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 3178 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1420 ANIMAL NUMBER: C92961 SEX: FEMALE DATE OF DEATH: 03/10/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 03/10/00 10:56 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 691 STUDY WEEK OF DEATH: 99 TERMINAL BODY WEIGHT: 621.8 GRAMS PROSECTOR: TODD ACKER RECORDER: TONE EVERTS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), HEMATO NEOPLASIA (HN) 3179 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1421 ANIMAL NUMBER: C92962 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 252.6 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 11:17 PROSECTOR: STEVE VAN ADESTINE RECORDER: KIMBERLY DURLAND POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) LF ADRENAL (AD0) RT ADRENAL (AD1) BRAIN (BR) LF KIDNEY (KD0) RT KIDNEY (KD1) LIVER (LI) LUNG (LU) LF OVARY (OV0) RT OVARY (OV1) SPLEEN (SP) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) UTERUS (UT) .0267 .0248 2.1071 .9178 .9077 7.6198 1.4824 .0547 .0614 .4245 .0071 .0086 .4418 .0106 % .0098 % .8342 % .3633 % .3593 % 3.0165 % .5869 % .0217 % .0243 % .1681 % .0028 % .0034 % .1749 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0127 .0118 1.0000 .4356 .4308 3.6162 .7035 .0260 .0291 .2015 .0034 .0041 .2097 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -NECROSIS, INDIVIDUAL HEPATOCYTE,MINIMAL -VACUOLATION, HEPATOCELLULAR, MIDZONAL / CENTRILOBULAR,-SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 3180 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1422 ANIMAL NUMBER: C92962 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 252.6 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 11:17 PROSECTOR: STEVE VAN ADESTINE RECORDER: KIMBERLY DURLAND POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV) 3181 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1423 ANIMAL NUMBER: C92963 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/04/00 STUDY DAY OF DEATH: 656 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: 332.3 GRAMS DATE AND TIME OF NECROPSY: 02/04/00 13:49 PROSECTOR: TONY HANFELD RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); VENTRAL-ABDOMINAL-RIGHT; WEEK 68; 60 X 50MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); AXILLARY REGION-LEFT; WEEK 72; 30 X 30MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK 83; 15 X 15MM; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; BLACK SKIN; VENTRALABDOMINAL-RIGHT; VERIFIED AT NECROPSY: NOT OBSERVED >SKIN & PELAGE; SORE/SCAB; VENTRALABDOMINAL-RIGHT; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; PALE; ENTIRE BODY; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) HISTOPATHOLOGY BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -NECROSIS, COAGULATIVE,-MINIMAL -HEMATOPOIESIS, EXTRAMEDULLARY,-SLIGHT -HYPERTROPHY, HEPATOCELLULAR, PERIPORTAL,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATELY SEVERE ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATE -HYPERPLASIA, CORTICAL,-MINIMAL -PIGMENT,-SLIGHT MARROW, FEMUR (FM) : -HYPERPLASIA, ERYTHROID, MARROW,MODERATELY SEVERE MARROW, STERNUM (SE) : -HYPERPLASIA, ERYTHROID, MARROW,MODERATE 3182 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1424 ANIMAL NUMBER: C92963 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/04/00 STUDY DAY OF DEATH: 656 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: 332.3 GRAMS DATE AND TIME OF NECROPSY: 02/04/00 13:49 PROSECTOR: TONY HANFELD RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 10 X 6 X 5 MM, RED, DARK RED, TAN, AND SOFT MAMMARY, FEMALE (MF) : -MASS(ES); LEFT AXILLARY REGION: SINGLE MASS, 33 X 28 X 18 MM, RED, DARK RED, TAN, FIRM, AND LOBULATED; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (AXILLARY) NORMAL (CAP 21) -MASS #2; LEFT INGUINAL REGION: SINGLE MASS, 30 X 18 X 15 MM, TAN, FIRM, AND LOBULATED; SOME SECTIONS EXUDE A TAN, CASEOUS MATERIAL; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED -MASS #3; RIGHT INGUINAL REGION: SINGLE MASS, 73 X 45 X 25 MM, RED, DARK RED, TAN, LOBULATED AND FIRM; SOME SECTIONS EXUDE A LIGHT BROWN, CASEOUS SEMISOLID, OTHERS EXUDE A DARK BROWN FLUID; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED; OVERLYING SKIN HAS A SINGLE DARK RED AND TAN ULCERATED AREA, 20 MM IN DIAMETER; COLLECTED INTACT WITH MASS THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -M-CARCINOMA,- 2 NEOPLASMS PRESENT -B-FIBROADENOMA,-NEOPLASM PRESENT >NOTE:>MASS #3 IS THE FIBROADENOMA. MASS #2 HAS CONSIDERABLE SQUAMOUS METAPLASIA 3183 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1425 ANIMAL NUMBER: C92963 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/04/00 STUDY DAY OF DEATH: 656 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: 332.3 GRAMS DATE AND TIME OF NECROPSY: 02/04/00 13:49 PROSECTOR: TONY HANFELD RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY UTERUS (UT) : -CYST(S); BILATERAL, WITHIN WALLS: MULTIPLE CLEAR FLUID FILLED CYSTS, UP TO 3 MM IN DIAMETER GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL UPPER INCISORS UTERUS (UT) : -HYPERPLASIA, CYSTIC, ENDOMETRIAL,MODERATELY SEVERE CAUSE OF DEATH (CD) : -NEOPLASM, MAMMARY GLAND,-PRESENT LN, AXILLARY (AX) : -HYPERPLASIA, LYMPHOPLASMACYTIC,MODERATE 3184 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1426 ANIMAL NUMBER: C92963 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/04/00 STUDY DAY OF DEATH: 656 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: 332.3 GRAMS DATE AND TIME OF NECROPSY: 02/04/00 13:49 PROSECTOR: TONY HANFELD RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUm /s KULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 3185 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1427 ANIMAL NUMBER: C92964 SEX: FEMALE DATE OF DEATH: 04/19/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/19/00 12:55 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 494.9 GRAMS PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); VENTRAL-CERVICAL; WEEK 83; 30 X 30MM; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY ADRENAL, CORTEX (AC) : -LARGE; LEFT: 10 X 8 X 6 MM (RIGHT IS 6 X 4 X 4 MM) PITUITARY (PI) : -LARGE; 7 X 6 X 4 MM; APPEARS SWOLLEN STOMACH, GL (ST) : -THICKENED WALL; DIFFUSE HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-SLIGHT -HYPERPLASIA, BILE DUCT,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, PELVIC,-MINIMAL ADRENAL, CORTEX (AC) : -HYPERTROPHY, CORTICAL CELL,-MINIMAL -PIGMENT,-MINIMAL ADRENAL, MEDULLA (MA) : -B-PHEOCHROMOCYTOMA,-NEOPLASM PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT -HYPERPLASIA,-SLIGHT STOMACH, GL (ST) : >UNREMARKABLE MAMMARY, FEMALE (MF) : -M-CARCINOMA,-NEOPLASM PRESENT -B-FIBROADENOMA,-NEOPLASM PRESENT >NOTE:>MASS #2 IS THE FIBROADENOMA 3186 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1428 ANIMAL NUMBER: C92964 SEX: FEMALE DATE OF DEATH: 04/19/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/19/00 12:55 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 494.9 GRAMS PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS(ES); MID VENTRAL CERVICAL REGION: SINGLE MASS, 35 X 30 X 20 MM, TAN, RED, FIRM AND CYSTIC; SOME AREAS EXUDE A CLEAR FLUID; COLLECTED; REGIONAL LN (MANDIBULAR) NORMAL; COLLECTED -MASS #2; LEFT INGUINAL REGION: SINGLE MASS, 32 X 22 X 10 MM, TAN AND FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED UTERUS (UT) : -CYST(S); BILATERAL, WITHIN WALLS: MULTIPLE CLEAR FLUID FILLED CYSTS, UP TO 4 MM IN DIAMETER GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -MALOCCLUSION; BILATERAL, UPPER INCISORS UTERUS (UT) : -HYPERPLASIA, CYSTIC, ENDOMETRIAL,MINIMAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MANDIBULAR (MN) : -HYPERPLASIA, LYMPHOPLASMACYTIC,MODERATELY SEVERE 3187 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1429 ANIMAL NUMBER: C92964 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 494.9 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 12:55 PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), LUNG (LU), THYROID (TY), PARATHYROID (PT), URINARY BLADDER (UB) 3188 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1430 ANIMAL NUMBER: C92965 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/13/99 STUDY DAY OF DEATH: 573 STUDY WEEK OF DEATH: 82 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/13/99 7:58 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); VENTRAL-ABDOMINAL; WEEK 60; 45 X 60MM; VERIFIED AT NECROPSY: OBSERVED >EYES; RED DISCHARGE; EYES; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; HUNCHED; VERIFIED AT NECROPSY: NOT APPLICABLE >EXCRETION; NO FECES; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LUNG (LU) : -UNCOLLAPSED; ALL LOBES HISTOPATHOLOGY BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC / HEMORRHAGIC,MINIMAL -PIGMENT,-MINIMAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),SLIGHT LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL HEART (HT) : -CARDIOMYOPATHY,-MINIMAL MUSCLE, SKELETAL (SM) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL 3189 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1431 ANIMAL NUMBER: C92965 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/13/99 STUDY DAY OF DEATH: 573 STUDY WEEK OF DEATH: 82 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/13/99 7:58 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 9 X 8 X 8 X MM, DARK RED, GREY, TAN, LOBULATED AND SOFT SKIN (SK) : >NOTE:>DUE TO MASS ON LEFT INGUINAL REGION, ROUTINE SECTION TAKEN FROM RIGHT MAMMARY, FEMALE (MF) : -MASS(ES); LEFT INGUINAL REGION: SINGLE MASS, 58 X 43 X 42 MM, RED, GREY, TAN, VASCULAR AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (INGUINAL) NORMAL (CAP 21) -CYST(S); RIGHT INGUINAL REGION: SINGLE WHITE, CREAMY SEMIFLUID FILLED CYST, 5 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION (SEE NECROPSY NOTES) >NOTE:>DUE TO MASS ON LEFT INGUINAL REGION, ROUTINE SECTION TAKEN FROM RIGHT THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT ILEUM (IL) : >AUTOLYTIC MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT -B-FIBROADENOMA,-NEOPLASM PRESENT -HYPERPLASIA, GLANDULAR,-SLIGHT VAGINA (VA) : -LUMEN, EXUDATE,-PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 3190 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1432 ANIMAL NUMBER: C92965 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/13/99 STUDY DAY OF DEATH: 573 STUDY WEEK OF DEATH: 82 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/13/99 7:58 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -DISCHARGE-OCULAR; BILATERAL: DRY, RED MATERIAL -ANIMAL THIN THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), KIDNEY (KD), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), HEMATO NEOPLASIA (HN), LN, INGUINAL (IN) 3191 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1433 ANIMAL NUMBER: C92966 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/03/99 STUDY DAY OF DEATH: 502 STUDY WEEK OF DEATH: 72 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 09/03/99 15:08 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; ROUGH HAIR COAT; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; HEAD TILT; RIGHT; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; ATAXIC; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 10 X 7 X 6 MM, RED, DARK RED, TAN AND FIRM GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN HISTOPATHOLOGY BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-MODERATE -FIBROSIS, PERIBILIARY,-SLIGHT -HYPERTROPHY, HEPATOCELLULAR, PERIPORTAL,-MINIMAL KIDNEY (KD) : -CYST,-PRESENT SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT ADRENAL, CORTEX (AC) : -PIGMENT,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 3192 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1434 ANIMAL NUMBER: C92966 SEX: FEMALE DATE OF DEATH: 09/03/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 09/03/99 15:08 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 502 STUDY WEEK OF DEATH: 72 TERMINAL BODY WEIGHT: NOT ENTERED PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 3193 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1435 ANIMAL NUMBER: C92967 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 285.4 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 10:07 PROSECTOR: DONIA MEYER RECORDER: KIMBERLY DURLAND POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) LF ADRENAL (AD0) RT ADRENAL (AD1) BRAIN (BR) LF KIDNEY (KD0) RT KIDNEY (KD1) LIVER (LI) LUNG (LU) LF OVARY (OV0) RT OVARY (OV1) SPLEEN (SP) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) UTERUS (UT) .0362 .0327 1.8011 1.1299 1.1418 8.5559 1.4342 .0683 .0575 .5138 .0095 .0090 .8051 .0127 % .0115 % .6311 % .3959 % .4001 % 2.9979 % .5025 % .0239 % .0201 % .1800 % .0033 % .0032 % .2821 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0201 .0182 1.0000 .6273 .6339 4.7504 .7963 .0379 .0319 .2853 .0053 .0050 .4470 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 3194 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1436 ANIMAL NUMBER: C92967 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 285.4 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 10:07 PROSECTOR: DONIA MEYER RECORDER: KIMBERLY DURLAND POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV) 3195 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1437 ANIMAL NUMBER: C92968 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 08/09/99 STUDY DAY OF DEATH: 477 STUDY WEEK OF DEATH: 69 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 08/09/99 14:51 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >EYES; DILATED PUPIL; EYES; VERIFIED AT NECROPSY: NOT APPLICABLE >EYES; RED DISCHARGE; EYES; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LUNG (LU) : -UNCOLLAPSED; ALL LOBES HISTOPATHOLOGY EYE (EY) : -INFLAMMATION, ACUTE,-SLIGHT BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -HEMORRHAGE,-MINIMAL -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL -THROMBUS,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-MODERATE SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MINIMAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATE -PIGMENT,-MINIMAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MINIMAL LUNG (LU) : -CONGESTION,-PRESENT 3196 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1438 ANIMAL NUMBER: C92968 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 08/09/99 STUDY DAY OF DEATH: 477 STUDY WEEK OF DEATH: 69 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 08/09/99 14:51 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 15 X 10 X 10 MM, RED, DARK RED, GREY, TAN, LOBULATED AND SOFT GENERAL COMMENT (GC) : -DISCHARGE-OCULAR; BILATERAL: DRY, RED MATERIAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT JEJUNUM (JE) : >AUTOLYTIC ILEUM (IL) : >AUTOLYTIC CECUM (CE) : >AUTOLYTIC MAMMARY, FEMALE (MF) : -HYPERPLASIA, GLANDULAR,-SLIGHT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 3197 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1439 ANIMAL NUMBER: C92968 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 08/09/99 STUDY DAY OF DEATH: 477 STUDY WEEK OF DEATH: 69 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 08/09/99 14:51 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 3198 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1440 ANIMAL NUMBER: C92969 SEX: FEMALE DATE OF DEATH: 05/15/98 STUDY DAY OF DATE AND TIME OF NECROPSY: 05/15/98 11:08 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 178.3 GRAMS PROSECTOR: LYNN HORNER RECORDER: DEBBIE PIRKEL PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN STATUS LF ADRENAL (AD0) RT ADRENAL (AD1) BRAIN (BR) LF KIDNEY (KD0) RT KIDNEY (KD1) LIVER (LI) LUNG (LU) LF OVARY (OV0) RT OVARY (OV1) SPLEEN (SP) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) UTERUS (UT) ------------------------------------ 6.3530 -------------------------------------------------- ------------------------------------ 3.5640 % -------------------------------------------------- -------------------------------------------------------------------------------------------- NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN WEIGHT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS 3199 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1441 ANIMAL NUMBER: C92969 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 178.3 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 11:08 PROSECTOR: LYNN HORNER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) 3200 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1442 ANIMAL NUMBER: C92970 SEX: FEMALE DATE OF DEATH: 04/19/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/19/00 7:43 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 344.8 GRAMS PROSECTOR: DAVID SCHUETTE RECORDER: LAURIE J. SCHULLER PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; SORE/SCAB; PAW-HINDRIGHT; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: NOT OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) ADRENAL, CORTEX (AC) : -LARGE; LEFT: 6 X 4 X 4 MM (RIGHT IS 5 X 3 X 3 MM) -MOTTLED; LEFT: DARK BROWN AND TAN OVARY (OV) : -CYST(S); BILATERAL: SINGLE CLEAR FLUID FILLED CYST ON EACH, LEFT IS 5 MM IN DIAMETER, RIGHT IS 10 MM IN DIAMETER HISTOPATHOLOGY BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-SLIGHT -FIBROSIS, PERIBILIARY,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, PELVIC,-MINIMAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT -PIGMENT,-SLIGHT OVARY (OV) : -CYST,-PRESENT LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL 3201 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1443 ANIMAL NUMBER: C92970 SEX: FEMALE DATE OF DEATH: 04/19/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/19/00 7:43 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 344.8 GRAMS PROSECTOR: DAVID SCHUETTE RECORDER: LAURIE J. SCHULLER PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 10 X 8 X 6 MM, TAN, DARK RED, RED, AND SOFT FOOT/FOOTPAD (FP) : -ABRASION(S)/ULCERATION(S); RIGHT HINDFOOT: SINGLE MOIST TAN ULCERATED AREA, 7 MM IN DIAMETER; COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -HYPERPLASIA, GLANDULAR,-MODERATELY SEVERE CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT FOOT/FOOTPAD (FP) : -INFLAMMATION, CHRONIC-ACTIVE,MODERATELY SEVERE -ACANTHOSIS / HYPERKERATOSIS,-MODERATE -FIBROSIS,-MODERATELY SEVERE 3202 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1444 ANIMAL NUMBER: C92970 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 344.8 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 7:43 PROSECTOR: DAVID SCHUETTE RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), ADRENAL, MEDULLA (MA), PARATHYROID (PT), URINARY BLADDER (UB) 3203 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1445 ANIMAL NUMBER: C92971 SEX: FEMALE DATE OF DEATH: 01/03/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 01/03/00 11:07 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 624 STUDY WEEK OF DEATH: 90 TERMINAL BODY WEIGHT: 564.5 GRAMS PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); INGUINAL REGION-LEFT; WEEK 67; 110 X 110MM; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; VENTRALABDOMINAL-LEFT; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -DIFFUSELY LIGHT; ALL LOBES: TAN KIDNEY (KD) : -CYST(S); LEFT, MEDULLA: SINGLE CLEAR FLUID FILLED CYST, 3 MM IN DIAMETER HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -NECROSIS, INDIVIDUAL HEPATOCYTE,MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-SLIGHT -NECROSIS, COAGULATIVE,-MINIMAL -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, PERIPORTAL,-MODERATE KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, PELVIC,-SLIGHT -HYPERPLASIA, TRANSITIONAL CELL,-SLIGHT SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATELY SEVERE PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-SLIGHT 3204 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1446 ANIMAL NUMBER: C92971 SEX: FEMALE DATE OF DEATH: 01/03/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 01/03/00 11:07 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 624 STUDY WEEK OF DEATH: 90 TERMINAL BODY WEIGHT: 564.5 GRAMS PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY ADRENAL, CORTEX (AC) : -LARGE; BILATERAL: EACH IS 9 X 7 X 7 MM PITUITARY (PI) : -MASS(ES); 9 X 9 X 8 MM, RED, TAN, AND SOFT PANCREAS (PA) : -HYPERPLASIA, ISLET CELLS,-SLIGHT ADRENAL, CORTEX (AC) : -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL -DEGENERATION, CYSTIC / HEMORRHAGIC,MODERATE MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,MODERATELY SEVERE MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,MODERATELY SEVERE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATELY SEVERE LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,SLIGHT HEART (HT) : -CARDIOMYOPATHY,-MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-SLIGHT 3205 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1447 ANIMAL NUMBER: C92971 SEX: FEMALE DATE OF DEATH: 01/03/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 01/03/00 11:07 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 624 STUDY WEEK OF DEATH: 90 TERMINAL BODY WEIGHT: 564.5 GRAMS PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS(ES); LEFT INGUINAL REGION: SINGLE MASS, 140 X 130 X 50 MM (303.4 GRAMS), RED, TAN, GRAY, LOBULATED AND FIRM; CUT SURFACE CONTAINS A TAN CREAMY SEMIFLUID; PORTION COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED; OVERLYING SKIN HAS A SINGLE DARK RED ABRADED AREA, 50 MM IN DIAMETER; COLLECTED INTACT UTERUS (UT) : -CYST(S); RIGHT HORN, WITHIN WALL: MULTIPLE CLEAR FLUID FILLED CYSTS, UP TO 3 MM IN DIAMETER GENERAL COMMENT (GC) -BLOOD FILM TAKEN -MALOCCLUSION -ANIMAL THIN : MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT -B-FIBROADENOMA,-NEOPLASM PRESENT UTERUS (UT) : -HYPERPLASIA, CYSTIC, ENDOMETRIAL,MODERATE CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 3206 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1448 ANIMAL NUMBER: C92971 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/03/00 STUDY DAY OF DEATH: 624 STUDY WEEK OF DEATH: 90 TERMINAL BODY WEIGHT: 564.5 GRAMS DATE AND TIME OF NECROPSY: 01/03/00 11:07 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 3207 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1449 ANIMAL NUMBER: C92972 SEX: FEMALE DATE OF DEATH: 02/18/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 02/18/00 8:45 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 670 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: 272.9 GRAMS PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >EYES; RED DISCHARGE; EYES; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); SHOULDER-RIGHT; WEEK 72; 55 X 40MM; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) ADRENAL, CORTEX (AC) : -LARGE; BILATERAL: EACH IS 6 MM IN DIAMETER PITUITARY (PI) : -MASS(ES) ; SINGLE MASS, 11 X 10 X 9 MM, RED, DARK RED,. TAN, LOBULATED, AND SOFT HISTOPATHOLOGY BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, MACROPHAGE, PIGMENTED,SLIGHT KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-MODERATE ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATE -PIGMENT,-SLIGHT ADRENAL, MEDULLA (MA) : -B-PHEOCHROMOCYTOMA,-NEOPLASM PRESENT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT 3208 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1450 ANIMAL NUMBER: C92972 SEX: FEMALE DATE OF DEATH: 02/18/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 02/18/00 8:45 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 670 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: 272.9 GRAMS PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT DORSAL SHOULDER REGION: SINGLE MASS, 55 X 40 X 35 MM, RED, TAN, VASCULAR AND FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (AXILLARY) NORMAL (CAP 21) VAGINA (VA) : -SEMIFLUID MATERIAL; WITHIN LUMEN: LIGHT GREEN CREAMY SEMIFLUID MATERIAL GENERAL COMMENT (GC) -BLOOD FILM TAKEN -DISCHARGE-OCULAR; AND DRY -ANIMAL THIN : BILATERAL: DARK RED HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT -B-FIBROADENOMA,-NEOPLASM PRESENT VAGINA (VA) : >UNREMARKABLE CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 3209 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1451 ANIMAL NUMBER: C92972 SEX: FEMALE DATE OF DEATH: 02/18/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 02/18/00 8:45 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 670 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: 272.9 GRAMS PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), HEMATO NEOPLASIA (HN), LN, AXILLARY (AX) 3210 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1452 ANIMAL NUMBER: C92973 SEX: FEMALE DATE OF DEATH: 04/19/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/19/00 10:51 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 610.3 GRAMS PROSECTOR: TONY HANFELD RECORDER: LAURIE J. SCHULLER PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -MASS(ES); ALONG MARGIN OF LEFT LATERAL LOBE: SINGLE MASS, 7 X 3 X 3 MM, RED AND TAN; SAME ON CUT SURFACE (CAP 22); REGIONAL LN (HEPATIC) NOT IDENTIFIED LN, MESENTERIC (MS) : -DIFFUSELY RED HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-SLIGHT -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL -HYPERPLASIA, BILE DUCT,-SLIGHT -FIBROSIS, PERIBILIARY,-SLIGHT -B-ADENOMA, HEPATOCELLULAR,-NEOPLASM PRESENT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, PELVIC,-MINIMAL PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-SLIGHT LN, MESENTERIC (MS) : -HEMORRHAGE,-PRESENT LUNG (LU) : -N-CARCINOMA,-FROM THYROID THYROID (TY) : -M-CARCINOMA, C-CELL,-NEOPLASM PRESENT 3211 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1453 ANIMAL NUMBER: C92973 SEX: FEMALE DATE OF DEATH: 04/19/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/19/00 10:51 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 610.3 GRAMS PROSECTOR: TONY HANFELD RECORDER: LAURIE J. SCHULLER PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY THYROID (TY) : -MASS(ES); LEFT: SINGLE MASS, 10 MM IN DIAMETER, RED, TAN, AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (MEDIASTINAL) NOT IDENTIFIED PITUITARY (PI) : -MASS(ES); 10 X 6 X 4 MM, RED, DARK RED, LOBULATED, AND FIRM GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 3212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1454 ANIMAL NUMBER: C92973 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 610.3 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 10:51 PROSECTOR: TONY HANFELD RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: MAMMARY, FEMALE (MF), URINARY BLADDER (UB) 3213 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1455 ANIMAL NUMBER: C92974 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 332.1 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 7:50 PROSECTOR: JODI THIEREN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); VENTRAL-CERVICAL-RIGHT; WEEK 104; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) ADRENAL, CORTEX (AC) : -LARGE; BILATERAL: LEFT IS 9 MM IN DIAMETER, RIGHT IS 6 MM IN DIAMETER HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-SLIGHT -FIBROSIS, PERIBILIARY,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, PELVIC,-SLIGHT -INFLAMMATION, SUPPURATIVE, PELVIC,MINIMAL ADRENAL, CORTEX (AC) : -HYPERTROPHY, CORTICAL CELL,-MODERATELY SEVERE -DEGENERATION, CYSTIC / HEMORRHAGIC,SEVERE -PIGMENT,-MINIMAL ADRENAL, MEDULLA (MA) : -UNILATERALLY EXAMINED,-PRESENT THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL 3214 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1456 ANIMAL NUMBER: C92974 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 332.1 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 7:50 PROSECTOR: JODI THIEREN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 9 X 9 X 8 MM, DARK RED, RED, GRAY AND FIRM MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT PECTORAL REGION: SINGLE MASS, 17 X 8 X 6 MM, TAN AND FIRM; ON CUT SECTION EXUDES A TAN CREAMY SEMIFLUID; COLLECTED; REGIONAL LN (SEE AXILLARY); POSSIBLY CONSISTENT WITH MASS (VENTRAL-CERVICAL-RIGHT) AS NOTED IN CLINICAL OBSERVATIONS UTERUS (UT) : -CYST(S); LEFT, WITHIN WALL: SINGLE CLEAR FLUID FILLED CYST, 4 MM IN DIAMETER LN, AXILLARY (AX) : -MOTTLED; BILATERAL: BROWN AND TAN (CAP 22) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT -B-FIBROADENOMA,-NEOPLASM PRESENT -HYPERPLASIA, GLANDULAR,-SLIGHT URINARY BLADDER (UB) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL UTERUS (UT) : -DILATATION,-MINIMAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, AXILLARY (AX) : -HYPERPLASIA, LYMPHOPLASMACYTIC,MODERATE -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL 3215 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1457 ANIMAL NUMBER: C92974 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 332.1 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 7:50 PROSECTOR: JODI THIEREN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), LUNG (LU), PARATHYROID (PT) 3216 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1458 ANIMAL NUMBER: C92975 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 492.7 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 7:27 PROSECTOR: JAMES T. CALLEN RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); VENTRAL-ABDOMINAL; WEEK 89; 70 X 70MM; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) THYROID (TY) : -LARGE; LEFT: 6 X 4 X 3 MM (RIGHT IS 4 X 2 X 1 MM) PITUITARY (PI) : -MASS(ES); 9 X 6 X 5 MM, RED, DARK RED, TAN, AND FIRM HISTOPATHOLOGY BRAIN (BR) : >UNREMARKABLE LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MODERATELY SEVERE -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL -HYPERPLASIA, BILE DUCT,-SLIGHT -FIBROSIS, PERIBILIARY,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, PELVIC,-MINIMAL LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL THYROID (TY) : -B-ADENOMA, C-CELL,- 2 NEOPLASMS PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT 3217 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1459 ANIMAL NUMBER: C92975 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 492.7 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 7:27 PROSECTOR: JAMES T. CALLEN RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS(ES); EXTENDS FROM LEFT TO RIGHT INGUINAL REGIONS: SINGLE MASS, 100 X 75 X 35 MM, RED, TAN, LOBULATED, AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (INGUINAL) NOT IDENTIFIED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN MAMMARY, FEMALE (MF) : -B-FIBROADENOMA,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 3218 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1460 ANIMAL NUMBER: C92975 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 492.7 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 7:27 PROSECTOR: JAMES T. CALLEN RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), PARATHYROID (PT), URINARY BLADDER (UB) 3219 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1461 ANIMAL NUMBER: C92976 SEX: FEMALE DATE OF DEATH: 03/31/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 03/31/00 10:15 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 712 STUDY WEEK OF DEATH: 102 TERMINAL BODY WEIGHT: 453.7 GRAMS PROSECTOR: JERALD W. JOHNSON RECORDER: JERALD W. JOHNSON PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK 83; 50 X 50MM TWO MASSES APPEAR TO HAVE GROWN TOGETHER; VERIFIED AT NECROPSY: OBSERVED >EYES; RED DISCHARGE; EYE-LEFT; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; HEAD TILT; LEFT; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) KIDNEY (KD) : -GRANULAR MATERIAL; LEFT, WITHIN PELVIS: MULTIPLE TAN PINPOINT GRANULES HISTOPATHOLOGY EYE (EY) : -DEGENERATION, LENTICULAR,-MODERATELY SEVERE BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-SLIGHT -NECROSIS, INDIVIDUAL HEPATOCYTE,MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, PELVIC,-SLIGHT -HYPERPLASIA, TRANSITIONAL CELL,-SLIGHT SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SLIGHT PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-SLIGHT ADRENAL, CORTEX (AC) : -UNILATERALLY EXAMINED,-PRESENT -M-CARCINOMA,-NEOPLASM PRESENT 3220 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1462 ANIMAL NUMBER: C92976 SEX: FEMALE DATE OF DEATH: 03/31/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 03/31/00 10:15 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 712 STUDY WEEK OF DEATH: 102 TERMINAL BODY WEIGHT: 453.7 GRAMS PROSECTOR: JERALD W. JOHNSON RECORDER: JERALD W. JOHNSON PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY ADRENAL, CORTEX (AC) : -MASS(ES); INVOLVING ENTIRE RIGHT ADRENAL: SINGLE MASS, 25 X 20 X 17 MM, DARK RED, RED, TAN AND SOFT; SAME ON CUT SURFACE; COLLECTED AS ROUTINE SECTION; REGIONAL LN (RENAL) NORMAL (CAP 23) PITUITARY (PI) : -MASS(ES); 14 X 7 X 6 MM, DARK RED, RED, TAN AND SOFT ADRENAL, MEDULLA (MA) : -UNILATERALLY EXAMINED,-PRESENT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MINIMAL LUNG (LU) : -N-CARCINOMA,-FROM ADRENAL, CORTEX HEART (HT) : -CARDIOMYOPATHY,-MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-MODERATE -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT -B-FIBROADENOMA,-NEOPLASM PRESENT 3221 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1463 ANIMAL NUMBER: C92976 SEX: FEMALE DATE OF DEATH: 03/31/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 03/31/00 10:15 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 712 STUDY WEEK OF DEATH: 102 TERMINAL BODY WEIGHT: 453.7 GRAMS PROSECTOR: JERALD W. JOHNSON RECORDER: JERALD W. JOHNSON PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS(ES); LEFT INGUINAL REGION: SINGLE MASS, 50 X 40 X 30 MM, RED, TAN, BROWN, LOBULATED AND FIRM; SAME ON CUT SURFACE; PORTION COLLECTED; REGIONAL LN (SEE INGUINAL) LN, INGUINAL (IN) : -LARGE; LEFT: 5 X 4 X 2 MM (RIGHT IS 3 X 2 X 1 MM) (CAP 21) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DARK RED DRY MATERIAL CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT LN, INGUINAL (IN) : -HYPERPLASIA, LYMPHOPLASMACYTIC,-SLIGHT LN, RENAL (RL) : -INFILTRATE, MACROPHAGE, PIGMENTED,SLIGHT 3222 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1464 ANIMAL NUMBER: C92976 SEX: FEMALE DATE OF DEATH: 03/31/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 03/31/00 10:15 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 712 STUDY WEEK OF DEATH: 102 TERMINAL BODY WEIGHT: 453.7 GRAMS PROSECTOR: JERALD W. JOHNSON RECORDER: JERALD W. JOHNSON PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 3223 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1465 ANIMAL NUMBER: C92977 SEX: FEMALE DATE OF DEATH: 03/05/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 03/05/00 13:17 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DEATH: 686 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: NOT ENTERED PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; SORE/SCAB; PAW-HINDRIGHT; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -MOTTLED; ALL LOBES, DIFFUSE: RED AND TAN KIDNEY (KD) : -LARGE PELVIS(ES); BILATERAL -GRANULAR MATERIAL; BILATERAL, WITHIN PELVIS: MULTIPLE HARD TAN CALCULI HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MODERATELY SEVERE -DEGENERATION / NECROSIS, CENTRILOBULAR,-MINIMAL -LEUKOCYTOSIS, SINUSOIDAL,-MODERATE KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MODERATE -DILATATION, PELVIC,-MODERATE -MINERALIZATION, PELVIC,-MINIMAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATELY SEVERE PANCREAS (PA) : -INFILTRATION, FAT, INTERSTITIAL,MODERATELY SEVERE ADRENAL, CORTEX (AC) : -HEMORRHAGE,-PRESENT 3224 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1466 ANIMAL NUMBER: C92977 SEX: FEMALE DATE OF DEATH: 03/05/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 03/05/00 13:17 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DEATH: 686 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: NOT ENTERED PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY ADRENAL, CORTEX (AC) : -MASS(ES); LEFT, REPLACES ENTIRE ADRENAL: SINGLE MASS, 26 X 32 X 45 MM, SOFT, RED, DARK RED, TAN AND LOBULATED; SAME ON CUT SURFACE; REGIONAL LN (RENAL) NOT IDENTIFIED ADRENAL, MEDULLA (MA) : -B-PHEOCHROMOCYTOMA,-NEOPLASM PRESENT MARROW, FEMUR (FM) : -HYPERPLASIA, ERYTHROID, MARROW,-SEVERE MARROW, STERNUM (SE) : -HYPERPLASIA, ERYTHROID, MARROW,MODERATELY SEVERE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),SLIGHT HEART (HT) : -CARDIOMYOPATHY,-SLIGHT JEJUNUM (JE) : >AUTOLYTIC ILEUM (IL) : >AUTOLYTIC CECUM (CE) : >AUTOLYTIC COLON (CO) : >AUTOLYTIC MAMMARY, FEMALE (MF) : -HYPERPLASIA, GLANDULAR,-SLIGHT 3225 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1467 ANIMAL NUMBER: C92977 SEX: FEMALE DATE OF DEATH: 03/05/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 03/05/00 13:17 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DEATH: 686 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: NOT ENTERED PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY FOOT/FOOTPAD (FP) : -CRUSTED AREA(S); RIGHT HINDFOOT: SINGLE DRY, DARK RED AREA, 5 X 7 MM; COLLECTED GENERAL COMMENT (GC) : -DISCHARGE-ORAL; CLEAR MOIST MATERIAL -STAINS - SKIN/HAIRCOAT; ENTIRE VENTRAL SURFACE: DRY RED MATERIAL VAGINA (VA) : -LUMEN, EXUDATE,-PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, ADRENAL,-PRESENT FOOT/FOOTPAD (FP) : -INFLAMMATION, CHRONIC-ACTIVE,-SEVERE -ULCERATION,-MODERATELY SEVERE -ACANTHOSIS / HYPERKERATOSIS,MODERATELY SEVERE -BACTERIA,-PRESENT 3226 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1468 ANIMAL NUMBER: C92977 SEX: FEMALE DATE OF DEATH: 03/05/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 03/05/00 13:17 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DEATH: 686 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: NOT ENTERED PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), HEMATO NEOPLASIA (HN) 3227 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1469 ANIMAL NUMBER: C92978 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 336.5 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 11:29 PROSECTOR: JODI THIEREN RECORDER: KIMBERLY DURLAND POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) LF ADRENAL (AD0) RT ADRENAL (AD1) BRAIN (BR) LF KIDNEY (KD0) RT KIDNEY (KD1) LIVER (LI) LUNG (LU) LF OVARY (OV0) RT OVARY (OV1) SPLEEN (SP) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) UTERUS (UT) .0367 .0305 2.0776 1.1629 1.1663 9.9351 1.4846 .0760 .0671 .5013 .0086 .0102 .5278 .0109 % .0091 % .6174 % .3456 % .3466 % 2.9525 % .4412 % .0226 % .0199 % .1490 % .0026 % .0030 % .1568 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0177 .0147 1.0000 .5597 .5614 4.7820 .7146 .0366 .0323 .2413 .0041 .0049 .2540 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 3228 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1470 ANIMAL NUMBER: C92978 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 336.5 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 11:29 PROSECTOR: JODI THIEREN RECORDER: KIMBERLY DURLAND POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV) 3229 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1471 ANIMAL NUMBER: C92979 SEX: FEMALE DATE OF DEATH: 01/01/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 01/01/00 15:19 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 622 STUDY WEEK OF DEATH: 89 TERMINAL BODY WEIGHT: 359.1 GRAMS PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); AXILLARY REGION-RIGHT; WEEK 80; 40 X 35MM; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; NO FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >DISCHARGE; GENITAL; RED IN COLOR; VERIFIED AT NECROPSY: NOT OBSERVED >BEHAVIOR; COLD TO TOUCH; ENTIRE BODY; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -RED FOCUS(I)/AREA(S); DIAPHRAGMATIC SURFACE OF LEFT LATERAL LOBE: SINGLE DARK RED AREA, 6 MM IN DIAMETER; SUNKEN WITH A STELLATE PATTERN; COLLECTED ON ROUTINE SECTION HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-MODERATE -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL -MINERALIZATION, TUBULAR,-MINIMAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT -PIGMENT,-SLIGHT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),SEVERE THYROID (TY) : -HYPERPLASIA, C-CELL,-SLIGHT 3230 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1472 ANIMAL NUMBER: C92979 SEX: FEMALE DATE OF DEATH: 01/01/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 01/01/00 15:19 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 622 STUDY WEEK OF DEATH: 89 TERMINAL BODY WEIGHT: 359.1 GRAMS PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 8 X 7 X 6 MM, DARK RED AND SOFT STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA: SINGLE TAN ERODED AREA, 7 X 3 MM MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT AXILLARY REGION: SINGLE MASS, 40 X 35 X 20 MM, RED, DARK RED, TAN, GRAY, LOBULATED AND FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (AXILLARY) NORMAL (CAP 21) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -STAINS-PERINEUM/PERIANAL; BROWN AND MOIST YELLOW, PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, NONGL (SU) : -ACANTHOSIS / HYPERKERATOSIS,MODERATELY SEVERE -ULCERATION,-SLIGHT -INFLAMMATION, CHRONIC-ACTIVE,MODERATELY SEVERE MAMMARY, FEMALE (MF) : -B-FIBROADENOMA,-NEOPLASM PRESENT -HYPERPLASIA, GLANDULAR,-SLIGHT VAGINA (VA) : -LUMEN, EXUDATE,-PRESENT -HYPERPLASIA, MUCINOUS,-MODERATE CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 3231 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1473 ANIMAL NUMBER: C92979 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/01/00 STUDY DAY OF DEATH: 622 STUDY WEEK OF DEATH: 89 TERMINAL BODY WEIGHT: 359.1 GRAMS DATE AND TIME OF NECROPSY: 01/01/00 15:19 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), HEMATO NEOPLASIA (HN), LN, AXILLARY (HG), (AX) 3232 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1474 ANIMAL NUMBER: C92980 SEX: FEMALE DATE OF DEATH: 06/28/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 06/28/99 14:06 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 435 STUDY WEEK OF DEATH: 63 TERMINAL BODY WEIGHT: 369.7 GRAMS PROSECTOR: JERALD W. JOHNSON RECORDER: JERALD W. JOHNSON PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); AXILLARY REGION-LEFT; WEEK 38; 30 X 25MM; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; AXILLARY REGION-LEFT; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); LIMB-HIND-RIGHT; WEEK 55; 60 X 55MM; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -LARGE; ALL LOBES: MARGINS APPEAR ROUNDED -MOTTLED; ALL LOBES: RED AND TAN SPLEEN (SP) : -LARGE; 53 X 11 X 7 MM -DIFFUSELY LIGHT; LIGHT RED ADRENAL, CORTEX (AC) : -LARGE; BILATERAL: EACH IS 7 MM IN DIAMETER HISTOPATHOLOGY BRAIN (BR) : >UNREMARKABLE LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-SLIGHT -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, PERIPORTAL,-MODERATE -MITOSIS, HEPATOCELLULAR, INCREASED,MODERATE KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATELY SEVERE PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-MODERATE ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL 3233 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1475 ANIMAL NUMBER: C92980 SEX: FEMALE DATE OF DEATH: 06/28/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 06/28/99 14:06 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 435 STUDY WEEK OF DEATH: 63 TERMINAL BODY WEIGHT: 369.7 GRAMS PROSECTOR: JERALD W. JOHNSON RECORDER: JERALD W. JOHNSON PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -LARGE; 6 X 6 X 5 MM; APPEARS SWOLLEN -RED FOCUS(I)/AREA(S); RIGHT MID ASPECT: SINGLE DARK RED AREA, 3 X 2 MM STOMACH, GL (ST) : -RED FOCUS(I)/AREA(S); MUCOSA: MULTIPLE DARK RED AREAS, UP TO 2 X 1 MM MAMMARY, FEMALE (MF) : -MASS(ES); LEFT AXILLARY REGION: SINGLE MASS, 30 X 20 X 10 MM, TAN, RED AND FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (SEE AXILLARY); OVERLYING SKIN IS ULCERATED, RED AND TAN, 20 X 12 MM; COLLECTED INTACT WITH MASS -THICKENED; ALL REGIONS: DIFFUSE; COLLECTED ON ROUTINE SECTION MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,MODERATELY SEVERE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY) MINIMAL LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : >UNREMARKABLE MAMMARY, FEMALE (MF) : -M-CARCINOMA,- 2 NEOPLASMS PRESENT -CYST / GALACTOCELE,-PRESENT 3234 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1476 ANIMAL NUMBER: C92980 SEX: FEMALE DATE OF DEATH: 06/28/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 06/28/99 14:06 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 435 STUDY WEEK OF DEATH: 63 TERMINAL BODY WEIGHT: 369.7 GRAMS PROSECTOR: JERALD W. JOHNSON RECORDER: JERALD W. JOHNSON PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS #2; RIGHT INGUINAL REGION: SINGLE MASS, 70 X 40 X 35 MM, TAN, RED, DARK RED, VASCULAR AND FIRM; SAME ON CUT SURFACE; PORTION COLLECTED; REGIONAL LN (INGUINAL) NORMAL (CAP 21) LN, AXILLARY (AX) : -LARGE; LEFT: UP TO 9 X 7 X 3 MM (FOR COMPARISON, RIGHT IS UP TO 5 X 3 X 1 MM)(CAP 20) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL: LOWER INCISORS VAGINA (VA) : >TISSUE MISSING CAUSE OF DEATH (CD) : -NEOPLASM, MAMMARY GLAND,-PRESENT LN, AXILLARY (AX) : -HYPERPLASIA, LYMPHOPLASMACYTIC,MODERATE 3235 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1477 ANIMAL NUMBER: C92980 SEX: FEMALE DATE OF DEATH: 06/28/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 06/28/99 14:06 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 435 STUDY WEEK OF DEATH: 63 TERMINAL BODY WEIGHT: 369.7 GRAMS PROSECTOR: JERALD W. JOHNSON RECORDER: JERALD W. JOHNSON PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), HEMATO NEOPLASIA (HN), LN, INGUINAL (IN) 3236 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1478 ANIMAL NUMBER: C92981 SEX: FEMALE DATE OF DEATH: 09/03/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 09/03/99 8:11 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 502 STUDY WEEK OF DEATH: 72 TERMINAL BODY WEIGHT: 346.0 GRAMS PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; ROUGH HAIR COAT; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; RED HAIR COAT; HEADENTIRE; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; HEAD TILT; LEFT; VERIFIED AT NECROPSY: NOT APPLICABLE >EXCRETION; NO FECES; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 11 X 9 X 6 MM, RED, TAN, AND FIRM GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -STAINS - SKIN/HAIRCOAT; DRY RED MATERIAL ENTIRE HEAD: HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT PANCREAS (PA) : -HYPERTROPHY / BASOPHILIA, ACINAR CELL,MINIMAL ADRENAL, CORTEX (AC) : -PIGMENT,-SLIGHT LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT VAGINA (VA) : -HYPERPLASIA, MUCINOUS,-SLIGHT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 3237 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1479 ANIMAL NUMBER: C92981 SEX: FEMALE DATE OF DEATH: 09/03/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 09/03/99 8:11 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 502 STUDY WEEK OF DEATH: 72 TERMINAL BODY WEIGHT: 346.0 GRAMS PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), KIDNEY (KD), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), HEMATO NEOPLASIA (HN) (JE), 3238 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1480 ANIMAL NUMBER: C92982 SEX: FEMALE DATE OF DEATH: 05/15/98 STUDY DAY OF DATE AND TIME OF NECROPSY: 05/15/98 11:22 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 189.9 GRAMS PROSECTOR: LYNN HORNER RECORDER: DEBBIE PIRKEL PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN STATUS LF ADRENAL (AD0) RT ADRENAL (AD1) BRAIN (BR) LF KIDNEY (KD0) RT KIDNEY (KD1) LIVER (LI) LUNG (LU) LF OVARY (OV0) RT OVARY (OV1) SPLEEN (SP) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) UTERUS (UT) ------------------------------------ 6.7810 -------------------------------------------------- ------------------------------------ 3.5707 % -------------------------------------------------- -------------------------------------------------------------------------------------------- NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN WEIGHT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS 3239 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1481 ANIMAL NUMBER: C92982 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 189.9 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 11:22 PROSECTOR: LYNN HORNER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) 3240 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1482 ANIMAL NUMBER: C92983 SEX: FEMALE DATE OF DEATH: 04/19/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/19/00 9:44 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 372.3 GRAMS PROSECTOR: TONY HANFELD RECORDER: LAURIE J. SCHULLER PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); INGUINAL REGION-LEFT; WEEK 94; 45 X 50MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); VENTRAL-CERVICAL; WEEK 96; VERIFIED AT NECROPSY: NOT OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LN, MESENTERIC (MS) : -DIFFUSELY DARK; DARK GREEN PITUITARY (PI) : -MASS(ES); 10 X 8 X 7 MM, RED, DARK RED, TAN, AND SOFT SKIN (SK) : >NOTE:>ROUTINE SECTION COLLECTED FROM RIGHT INGUINAL REGION DUE TO MASS ON LEFT INGUINAL REGION HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-SLIGHT -HYPERPLASIA, BILE DUCT,-SLIGHT -FIBROSIS, PERIBILIARY,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, PELVIC,-SLIGHT LN, MESENTERIC (MS) : -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT SKIN (SK) : >NOT REQUIRED TO BE EXAMINED FOR ANIMAL 3241 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1483 ANIMAL NUMBER: C92983 SEX: FEMALE DATE OF DEATH: 04/19/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/19/00 9:44 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 372.3 GRAMS PROSECTOR: TONY HANFELD RECORDER: LAURIE J. SCHULLER PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS(ES); LEFT INGUINAL REGION: SINGLE MASS, 58 X 45 X 25 MM, RED, DARK RED, TAN, LOBULATED, AND FIRM; SOME SECTIONS EXUDE A TAN CREAMY SEMISOLID; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED -THICKENED; ALL REGIONS DIFFUSE: COLLECTED ON ROUTINE SECTION >NOTE:>ROUTINE SECTION COLLECTED FROM RIGHT INGUINAL REGION DUE TO MASS ON LEFT INGUINAL REGION GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT -B-FIBROADENOMA,-NEOPLASM PRESENT -HYPERPLASIA, GLANDULAR,-SLIGHT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 3242 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1484 ANIMAL NUMBER: C92983 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 372.3 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 9:44 PROSECTOR: TONY HANFELD RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), LUNG (LU), THYROID (TY), PARATHYROID (PT), URINARY BLADDER (UB) 3243 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1485 ANIMAL NUMBER: C92984 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 498.7 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 8:14 PROSECTOR: TODD ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); INGUINAL REGION-RIGHT; WEEK 75; 50 X 40MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); VENTRAL-ABDOMINAL-RIGHT; WEEK 75; 50 X 40MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); AXILLARY REGION-LEFT; WEEK 93; 15 X 10MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); VENTRAL-ABDOMINAL; WEEK 104; 15 X 15MM; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) HISTOPATHOLOGY BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -NECROSIS, INDIVIDUAL HEPATOCYTE,SLIGHT -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MODERATE -FIBROSIS, PERIBILIARY,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, PELVIC,-SLIGHT -MINERALIZATION, TUBULAR,-MINIMAL LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-MODERATE 3244 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1486 ANIMAL NUMBER: C92984 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 498.7 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 8:14 PROSECTOR: TODD ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 10 X 9 X 6 MM, RED, DARK RED, TAN, AND SOFT MAMMARY, FEMALE (MF) : -MASS(ES); LEFT AXILLARY REGION: SINGLE MASS, 20 X 18 X 8 MM, TAN AND SOFT; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (AXILLARY) NORMAL (CAP 22) -THICKENED; ALL REGIONS DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION -MASS #2; RIGHT INGUINAL REGION, EXTENDING INTO RIGHT PERIANAL REGION: MULTIPLE MASSES, UP TO 60 X 50 X 25 MM, ALL ARE TAN, RED, DARK RED, AND FIRM; ALL ARE SAME ON CUT SURFACE; PORTIONS COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT -B-FIBROADENOMA,- 2 NEOPLASMS PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, AXILLARY (AX) : -HYPERPLASIA, LYMPHOPLASMACYTIC,MODERATE 3245 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1487 ANIMAL NUMBER: C92984 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 498.7 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 8:14 PROSECTOR: TODD ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), PARATHYROID (PT), URINARY BLADDER (UB) 3246 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1488 ANIMAL NUMBER: C92985 SEX: FEMALE DATE OF DEATH: 02/28/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 02/28/00 15:08 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 680 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: 561.6 GRAMS PROSECTOR: DENNIS HOFFMAN RECORDER: TONE EVERTS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); AXILLARY REGION-RIGHT; WEEK 76; 80 X 80MM; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; MISSING; TEETH; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK 82; 20 X 20MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); VENTRAL-ABDOMINAL-RIGHT; WEEK 95; 20 X 20MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); VENTRAL-THORACIC-LEFT; WEEK 95; 10 X 10MM; VERIFIED AT NECROPSY: NOT OBSERVED >MASS(ES); INGUINAL REGION-RIGHT; WEEK 96; 25 X 25MM; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -MOTTLED; ALL LOBES, DIFFUSE: RED AND TAN HISTOPATHOLOGY BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-MODERATE -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MODERATE -HYPERPLASIA, BILE DUCT,-MINIMAL -FIBROSIS, PERIBILIARY,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, PELVIC,-MODERATE SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-MODERATE ADRENAL, CORTEX (AC) : -HYPERTROPHY, CORTICAL CELL,-SLIGHT 3247 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1489 ANIMAL NUMBER: C92985 SEX: FEMALE DATE OF DEATH: 02/28/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 02/28/00 15:08 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 680 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: 561.6 GRAMS PROSECTOR: DENNIS HOFFMAN RECORDER: TONE EVERTS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 8 X 7 X 5 MM, SOFT, RED, AND TAN MAMMARY, FEMALE (MF) : -MASS(ES); LEFT INGUINAL REGION: SINGLE MASS, 27 X 18 X 15 MM, FIRM, LOBULATED, RED, AND TAN; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED -MASS #2; LEFT INGUINAL REGION, CAUDAL TO MASS NUMBER ONE: SINGLE MASS, 37 X 25 X 20 MM, RED, TAN, BROWN, AND SOFT; ON SECTION SOME AREAS CONTAIN A TAN AND DRY SEMISOLID MATERIAL; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC / HEMORRHAGIC,MODERATE -HYPERPLASIA, CORTICAL,-MINIMAL -PIGMENT,-MINIMAL MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,-MODERATE MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,-MODERATE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -M-CARCINOMA,- 2 NEOPLASMS PRESENT -B-FIBROADENOMA,- 2 NEOPLASMS PRESENT -HYPERPLASIA, GLANDULAR,-MODERATE >NOTE:>MASS #'S 1 AND 5 ARE FIBROADENOMAS. MASS #'S 3 AND 4 ARE CARCINOMAS MASS #2: SEE CLITORAL GLAND FOR DIAGNOSIS 3248 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1490 ANIMAL NUMBER: C92985 SEX: FEMALE DATE OF DEATH: 02/28/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 02/28/00 15:08 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 680 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: 561.6 GRAMS PROSECTOR: DENNIS HOFFMAN RECORDER: TONE EVERTS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS #3; RIGHT INGUINAL REGION: SINGLE MASS, 22 X 14 X 11 MM, FIRM, RED, TAN, AND LOBULATED; SAME ON CUT SURFACE, COLLECTED; REGIONAL LN INGUINAL (NOT IDENTIFIED) -MASS #4; RIGHT INGUINAL, CAUDAL TO MASS NUMBER THREE: SINGLE MASS, 45 X 30 X 20 MM, FIRM, LOBULATED, RED, DARK RED, AND TAN; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED -MASS #5; RIGHT AXILLARY REGION: SINGLE MASS, 75 X 75 X 55 MM, FIRM, LOBULATED, RED, DARK RED, TAN, AND VASCULAR; SAME ON CUT SURFACE; COLLECTED; OVERLYING SKIN HAS A 30 MM IN DIAMETER DRY, BROWN CRUSTED AREA; COLLECTED INTACT WITH MASS; REGIONAL LN (AXILLARY) NORMAL (CAP 23) UTERUS (UT) : -CYST(S); BILATERAL, WITHIN WALLS: MULTIPLE CLEAR FLUID FILLED CYSTS, UP TO 3 MM IN DIAMETER VAGINA (VA) : -SEMIFLUID MATERIAL; WITHIN LUMEN: CLEAR MUCOID MATERIAL UTERUS (UT) : -DILATATION,-SLIGHT CERVIX (CV) : -HYPERPLASIA, MUCINOUS,-MODERATE VAGINA (VA) : -LUMEN, EXUDATE,-PRESENT -HYPERPLASIA, MUCINOUS,-SEVERE 3249 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1491 ANIMAL NUMBER: C92985 SEX: FEMALE DATE OF DEATH: 02/28/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 02/28/00 15:08 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 680 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: 561.6 GRAMS PROSECTOR: DENNIS HOFFMAN RECORDER: TONE EVERTS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; RIGHT UPPER INCISOR -TEETH MISSING/BROKEN/WORN; LEFT UPPER INCISOR: MISSING CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT LN, AXILLARY (AX) : -HYPERPLASIA, LYMPHOPLASMACYTIC,MODERATE CLITORAL GLAND (CL) : -ECTASIA, DUCTAL,-PRESENT -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE -HYPERKERATOSIS,-SEVERE 3250 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1492 ANIMAL NUMBER: C92985 SEX: FEMALE DATE OF DEATH: 02/28/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 02/28/00 15:08 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 680 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: 561.6 GRAMS PROSECTOR: DENNIS HOFFMAN RECORDER: TONE EVERTS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), HEMATO NEOPLASIA (HN) 3251 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1493 ANIMAL NUMBER: C92986 SEX: FEMALE DATE OF DEATH: 04/21/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/21/00 7:25 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 453.0 GRAMS PROSECTOR: DAVID SCHUETTE RECORDER: TONE EVERTS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); INGUINAL REGION-LEFT; WEEK 85; 70 X 60MM; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 8 X 8 X 5 MM, DARK RED, AND SOFT HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL -HYPERPLASIA, TRANSITIONAL CELL,MINIMAL PANCREAS (PA) : -ATROPHY, ACINAR,-MINIMAL -HYPERTROPHY / BASOPHILIA, ACINAR CELL,MINIMAL LUNG (LU) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT -B-FIBROADENOMA,- 2 NEOPLASMS PRESENT -B-ADENOMA,-NEOPLASM PRESENT 3252 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1494 ANIMAL NUMBER: C92986 SEX: FEMALE DATE OF DEATH: 04/21/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/21/00 7:25 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 453.0 GRAMS PROSECTOR: DAVID SCHUETTE RECORDER: TONE EVERTS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS(ES); LEFT INGUINAL REGION: SINGLE MASS, 100 X 65 X 30 MM (104.7 GRAMS), RED, TAN, LOBULATED, AND VASCULAR; CUT SURFACE EXUDES A TAN CREAMY SEMIFLUID MATERIAL; COLLECTED INTACT; REGIONAL LN (INGUINAL) NORMAL (CAP 22) -THICKENED; ALL REGIONS DIFFUSE: TAN; COLLECTED ON ROUTINE SECTION -MASS #2; RIGHT INGUINAL REGION: SINGLE MASS, 23 X 15 X 6 MM, TAN, AND FIRM, SAME ON CUT SURFACE; SOME SECTIONS EXUDE A TAN CASEOUS SEMIFLUID (CAP 23); REGIONAL LN (INGUINAL) NORMAL (CAP 22) UTERUS (UT) : -MASS(ES); LEFT HORN, WITHIN LUMEN: SINGLE MASS, 35 X 10 X 10 MM, RED, TAN, AND SOFT; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (LUMBAR) NORMAL (CAP 25) MAMMARY, FEMALE (MF) : >NOTE:>SMALL ADENOMA NOTED ON REGULAR MAMMARY GLAND SECTION MASSES 1 AND 2 WERE FIBROADENOMAS UTERUS (UT) : -B-POLYP, ENDOMETRIAL, STROMAL,NEOPLASM PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, INGUINAL (IN) : -HYPERPLASIA, LYMPHOPLASMACYTIC,-SLIGHT LN, LUMBAR/ILIAC (LM) : -HYPERPLASIA, LYMPHOPLASMACYTIC,-SLIGHT 3253 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1495 ANIMAL NUMBER: C92986 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 453.0 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 7:25 PROSECTOR: DAVID SCHUETTE RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUm /s KULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PARATHYROID (PT), URINARY BLADDER (UB) 3254 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1496 ANIMAL NUMBER: C92987 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 273.3 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 10:46 PROSECTOR: STEVE VAN ADESTINE RECORDER: KIMBERLY DURLAND POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) LF ADRENAL (AD0) RT ADRENAL (AD1) BRAIN (BR) LF KIDNEY (KD0) RT KIDNEY (KD1) LIVER (LI) LUNG (LU) LF OVARY (OV0) RT OVARY (OV1) SPLEEN (SP) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) UTERUS (UT) .0307 .0327 2.2943 1.1962 1.1435 8.9944 1.4595 .0578 .0622 .6245 .0100 .0135 .5091 .0112 % .0120 % .8395 % .4377 % .4184 % 3.2910 % .5340 % .0211 % .0228 % .2285 % .0037 % .0049 % .1863 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0134 .0143 1.0000 .5214 .4984 3.9203 .6361 .0252 .0271 .2722 .0044 .0059 .2219 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -NECROSIS, INDIVIDUAL HEPATOCYTE,MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MINIMAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 3255 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1497 ANIMAL NUMBER: C92987 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 273.3 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 10:46 PROSECTOR: STEVE VAN ADESTINE RECORDER: KIMBERLY DURLAND POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV) 3256 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1498 ANIMAL NUMBER: C92988 SEX: FEMALE DATE OF DEATH: 04/19/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/19/00 11:42 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 585.1 GRAMS PROSECTOR: GREGORY CODDINGTON RECORDER: TERRI L. WILLIAMS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -RED FOCUS(I)/AREA(S); RIGHT MEDIAN LOBE: MULTIPLE DARK RED AREAS, UP TO 3 X 2 MM; COLLECTED ON ROUTINE SECTION PITUITARY (PI) : -RED FOCUS(I)/AREA(S); LEFT ANTERIOR ASPECT: SINGLE DARK RED FOCUS, 1 MM IN DIAMETER HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-SLIGHT -HYPERPLASIA, BILE DUCT,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, TUBULAR,-MINIMAL PANCREAS (PA) : -INFILTRATION, FAT, INTERSTITIAL,MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PITUITARY (PI) : -HYPERPLASIA,-SLIGHT MAMMARY, FEMALE (MF) : -HYPERPLASIA, GLANDULAR,-SLIGHT 3257 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1499 ANIMAL NUMBER: C92988 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 585.1 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 11:42 PROSECTOR: GREGORY CODDINGTON RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN URINARY BLADDER (UB) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PARATHYROID (PT) 3258 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1500 ANIMAL NUMBER: C92989 SEX: FEMALE DATE OF DEATH: 11/22/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 11/22/99 8:37 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 582 STUDY WEEK OF DEATH: 84 TERMINAL BODY WEIGHT: 408.6 GRAMS PROSECTOR: TODD ACKER RECORDER: TODD ACKER PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; NO FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; BROWN HAIR COAT; VENTRAL-ABDOMINAL; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY KIDNEY (KD) : -LARGE PELVIS(ES); BILATERAL HISTOPATHOLOGY EYE (EY) : -INFLAMMATION, ACUTE,-MINIMAL BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -DILATATION, PELVIC,-MODERATE -MINERALIZATION, TUBULAR,-MINIMAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MINIMAL -HYPERTROPHY, CORTICAL CELL,-SLIGHT -PIGMENT,-MINIMAL MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,-SEVERE MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,-SEVERE 3259 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1501 ANIMAL NUMBER: C92989 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/22/99 STUDY DAY OF DEATH: 582 STUDY WEEK OF DEATH: 84 TERMINAL BODY WEIGHT: 408.6 GRAMS DATE AND TIME OF NECROPSY: 11/22/99 8:37 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -LARGE; 7 X 5 X 5 MM, APPEARS SWOLLEN STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: MULTIPLE BLACK AREAS, UP TO 3 X 2 MM MAMMARY, FEMALE (MF) : -MASS(ES); MID VENTRAL THORACIC REGION: SINGLE MASS, 10 X 8 X 6 MM, TAN AND SOFT; SAME ON CUT SURFACE (CAP 21); REGIONAL LN (AXILLARY) NORMAL (CAP 22) UTERUS (UT) : -LUMEN FILLED WITH FLUID; RIGHT HORN: FILLED WITH A BROWN FLUID THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATELY SEVERE HEART (HT) : -CARDIOMYOPATHY,-MODERATE THYROID (TY) : -HYPERPLASIA, C-CELL,-SLIGHT PITUITARY (PI) : -HYPERPLASIA,-SLIGHT -CYST,-PRESENT STOMACH, GL (ST) : -EDEMA,-SLIGHT -INFLAMMATION, ACUTE,-SLIGHT MAMMARY, FEMALE (MF) : -M-CARCINOMA,-NEOPLASM PRESENT -CYST / GALACTOCELE,-PRESENT -HYPERPLASIA, GLANDULAR,-SLIGHT URINARY BLADDER (UB) : -HYPERPLASIA, TRANSITIONAL CELL,-SLIGHT UTERUS (UT) : -DILATATION,-MODERATE -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT CERVIX (CV) : -M-SARCOMA, STROMAL,-NEOPLASM PRESENT 3260 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1502 ANIMAL NUMBER: C92989 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/22/99 STUDY DAY OF DEATH: 582 STUDY WEEK OF DEATH: 84 TERMINAL BODY WEIGHT: 408.6 GRAMS DATE AND TIME OF NECROPSY: 11/22/99 8:37 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY CERVIX (CV) : -MASS(ES); WITHIN LUMEN, EXTENDING INTO PROXIMAL UTERUS AND VAGINA: SINGLE MASS, 70 X 50 X 35 MM, DARK BROWN, RED, DARK RED, TAN, AND SOFT; SOME AREAS EXUDE A BROWN FLUID; COLLECTED INTACT; REGIONAL LN (LEFT LUMBAR) NORMAL, RIGHT NOT IDENTIFIED (CAP 23) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL: UPPER INCISORS -DISCHARGE-OCULAR; RIGHT: DARK RED AND DRY -URINARY BLADDER-LARGE LUMEN; 30 X 15 X 15 MM -STAINS - SKIN/HAIRCOAT; VENTRAL ABDOMINAL REGION, EXTENDING TO PERIANAL REGION: BROWN AND MOIST CAUSE OF DEATH (CD) : -NEOPLASM, UT/CV/VA/OV,-PRESENT LN, AXILLARY (AX) : -HYPERPLASIA, RETICULOENDOTHELIAL,SLIGHT LN, LUMBAR/ILIAC (LM) : -HYPERPLASIA, LYMPHOPLASMACYTIC,-SLIGHT 3261 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1503 ANIMAL NUMBER: C92989 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/22/99 STUDY DAY OF DEATH: 582 STUDY WEEK OF DEATH: 84 TERMINAL BODY WEIGHT: 408.6 GRAMS DATE AND TIME OF NECROPSY: 11/22/99 8:37 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), VAGINA (VA), HEMATO NEOPLASIA (HN) 3262 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1504 ANIMAL NUMBER: C92990 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/01/00 STUDY DAY OF DEATH: 653 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: 547.2 GRAMS DATE AND TIME OF NECROPSY: 02/01/00 14:05 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); AXILLARY REGION-LEFT; WEEK 73; 65 X 55MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); VENTRAL-CERVICAL-RIGHT; WEEK 89; 50 X 90MM; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) HISTOPATHOLOGY BRAIN (BR) : >UNREMARKABLE LIVER (LI) : -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, PELVIC,-MODERATE -HYPERPLASIA, TRANSITIONAL CELL,MODERATE SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATELY SEVERE PANCREAS (PA) : -B-ADENOMA, ISLET CELL,-NEOPLASM PRESENT ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT -HYPERTROPHY, CORTICAL CELL,-MINIMAL -HYPERPLASIA, CORTICAL,-MINIMAL -PIGMENT,-SLIGHT 3263 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1505 ANIMAL NUMBER: C92990 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/01/00 STUDY DAY OF DEATH: 653 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: 547.2 GRAMS DATE AND TIME OF NECROPSY: 02/01/00 14:05 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY OVARY (OV) : -CYST(S); RIGHT: SINGLE CLEAR FLUID FILLED CYST, 3 MM IN DIAMETER PITUITARY (PI) : -MASS(ES); 10 X 6 X 5 MM, RED, TAN AND FIRM SALIV GL, MANDIB (SG) : -MISSING/NOT IDENTIFIED; NOT IDENTIFIED: NOT POSITIVELY IDENTIFIED; POSSIBLY COLLECTED WITH CERVICAL MASS NOTED ADRENAL, MEDULLA (MA) : -B-PHEOCHROMOCYTOMA,-NEOPLASM PRESENT OVARY (OV) : -CYST,-PRESENT MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,MODERATELY SEVERE MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,MODERATELY SEVERE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY) MINIMAL HEART (HT) : -CARDIOMYOPATHY,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT SALIV GL, MANDIB (SG) : -M-SCHWANNOMA,-NEOPLASM PRESENT -UNILATERALLY EXAMINED,-PRESENT MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT -B-FIBROADENOMA,-NEOPLASM PRESENT -HYPERPLASIA, GLANDULAR,-SLIGHT 3264 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1506 ANIMAL NUMBER: C92990 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/01/00 STUDY DAY OF DEATH: 653 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: 547.2 GRAMS DATE AND TIME OF NECROPSY: 02/01/00 14:05 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS(ES); LEFT AXILLARY REGION: SINGLE MASS, 65 X 55 X 35 MM, TAN, LOBULATED AND SOFT; ON SECTION, EXUDES A TAN CREAMY SEMIFLUID; COLLECTED INTACT; REGIONAL LN (SEE AXILLARY) -MASS #2; EXTENDS LATERALLY ACROSS ENTIRE VENTRAL CERVICAL REGION: SINGLE MASS, 70 X 45 X 36 MM, TAN, RED, LOBULATED, VASCULAR AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (MANDIBULAR) NOT IDENTIFIED LN, AXILLARY (AX) : -MOTTLED; BILATERAL: RED AND TAN 21) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN (CAP MAMMARY, FEMALE (MF) : >NOTE:>MASS #1 IS A FIBROADENOMA SEE SALIVARY GLAND FOR DIAGNOSIS OF MASS #2 CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT LN, AXILLARY (AX) : -HYPERPLASIA, LYMPHOPLASMACYTIC,MODERATE 3265 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1507 ANIMAL NUMBER: C92990 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/01/00 STUDY DAY OF DEATH: 653 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: 547.2 GRAMS DATE AND TIME OF NECROPSY: 02/01/00 14:05 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS) FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL) URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LN, MESENTERIC (MS), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) (CE), 3266 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1508 ANIMAL NUMBER: C92991 SEX: FEMALE DATE OF DEATH: 04/07/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/07/00 7:23 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 719 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 568.0 GRAMS PROSECTOR: GREGORY CODDINGTON RECORDER: TONE EVERTS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: NOT OBSERVED >SKIN & PELAGE; SORE/SCAB; PAW-HINDRIGHT; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE DARK RED AREAS, UP TO 3 MM IN DIAMETER; COLLECTED ON ROUTINE SECTIONS HISTOPATHOLOGY BRAIN (BR) : >UNREMARKABLE LIVER (LI) : -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-MINIMAL -NECROSIS, INDIVIDUAL HEPATOCYTE,MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL -DEGENERATION, CYSTIC,-MINIMAL -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, PELVIC,-SLIGHT PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-MODERATE -B-ADENOMA, ISLET CELL,-NEOPLASM PRESENT 3267 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1509 ANIMAL NUMBER: C92991 SEX: FEMALE DATE OF DEATH: 04/07/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/07/00 7:23 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 719 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 568.0 GRAMS PROSECTOR: GREGORY CODDINGTON RECORDER: TONE EVERTS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 7 X 6 X 5 MM, RED, TAN, AND SOFT MAMMARY, FEMALE (MF) : -THICKENED; ALL REGIONS DIFFUSE: COLLECTED ON ROUTINE SECTION -CYST(S); RIGHT INGUINAL REGION: SINGLE DARK BROWN FLUID FILLED CYST, 40 X 25 X 10 MM (CAP 23) FOOT/FOOTPAD (FP) : -CRUSTED AREA(S); RIGHT HIND FOOT: SINGLE DRY, RED, AND TAN CRUSTED AREA, 4 X 3 MM; COLLECTED LN, ANT MES/PANC (AP) : -LARGE; UP TO 8 MM IN DIAMETER (CAP 24) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN PANCREAS (PA) : -INFILTRATION, FAT, INTERSTITIAL,MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT -HYPERPLASIA, GLANDULAR,-MODERATE -B-ADENOMA,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT FOOT/FOOTPAD (FP) : -INFLAMMATION, CHRONIC-ACTIVE,MODERATELY SEVERE -ULCERATION,-MINIMAL -ACANTHOSIS / HYPERKERATOSIS,-SLIGHT LN, ANT MES/PANC (AP) : >NOT REQUIRED TO BE EXAMINED FOR ANIMAL >NOTE:>SEE PANCREAS FOR DIAGNOSIS OF MASS 3268 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1510 ANIMAL NUMBER: C92991 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/07/00 STUDY DAY OF DEATH: 719 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 568.0 GRAMS DATE AND TIME OF NECROPSY: 04/07/00 7:23 PROSECTOR: GREGORY CODDINGTON RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -DISCHARGE-OCULAR; LEFT: DARK RED DRY MATERIAL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), AORTA (AO), CALVARIUM/SKULL (CA), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PARATHYROID (PT), URINARY BLADDER (UB) 3269 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1511 ANIMAL NUMBER: C92992 SEX: FEMALE DATE OF DEATH: 11/23/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 11/23/99 15:21 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 583 STUDY WEEK OF DEATH: 84 TERMINAL BODY WEIGHT: 372.2 GRAMS PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); DORSAL; WEEK 76; 20 X 20MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); INGUINAL REGION-RIGHT; WEEK 76; 40 X 40MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); AXILLARY REGION-LEFT; WEEK 78; 40 X 40MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); VENTRAL-CERVICAL; WEEK 79; 10 X 10MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); VENTRAL-THORACIC; WEEK 62; 30 X 30MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); INGUINAL REGION-LEFT; WEEK 81; 10 X 10MM; VERIFIED AT NECROPSY: OBSERVED >EYES; SQUINTED; PERI-ORBITAL-RIGHT; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) HISTOPATHOLOGY BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-MODERATE -ATROPHY, ACINAR,-MINIMAL ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-SLIGHT -ANGIECTASIS,-MODERATE -HYPERTROPHY, CORTICAL CELL,-MINIMAL -PIGMENT,-SLIGHT THYMUS (TH) : >SECTION EXAMINED; TISSUE NOT PRESENT LUNG (LU) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT 3270 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1512 ANIMAL NUMBER: C92992 SEX: FEMALE DATE OF DEATH: 11/23/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 11/23/99 15:21 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 583 STUDY WEEK OF DEATH: 84 TERMINAL BODY WEIGHT: 372.2 GRAMS PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 10 X 7 X 5 MM, DARK RED, TAN AND SOFT STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA: MULTIPLE TAN ERODED AREAS, UP TO 3 X 2 MM SKIN (SK) : -MASS(ES); LEFT DORSAL LUMBAR REGION: SINGLE MASS, 25 X 20 X 10 MM, TAN AND FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (SEE INGUINAL); POSSIBLY CONSISTENT WITH MASS (DORSAL) AS NOTED IN CLINICAL OBSERVATIONS MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT LATERAL CERVICAL REGION: SINGLE MASS, 20 X 12 X 12 MM, DARK RED, RED, TAN, BROWN AND SOFT; CUT SURFACE EXUDES A DARK RED SEMIFLUID; COLLECTED; REGIONAL LN (SEE MANDIBULAR) MUSCLE, SKELETAL (SM) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL STOMACH, GL (ST) : -HYPERPLASIA, EPITHELIAL, GLANDULAR,MODERATE STOMACH, NONGL (SU) : -ACANTHOSIS / HYPERKERATOSIS,-SLIGHT SKIN (SK) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -M-CARCINOMA,- 3 NEOPLASMS PRESENT -CYST / GALACTOCELE,-PRESENT -HYPERPLASIA, GLANDULAR,-MODERATE 3271 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1513 ANIMAL NUMBER: C92992 SEX: FEMALE DATE OF DEATH: 11/23/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 11/23/99 15:21 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 583 STUDY WEEK OF DEATH: 84 TERMINAL BODY WEIGHT: 372.2 GRAMS PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION -MASS #2; ALL REGIONS, DIFFUSE: MULTIPLE MASSES, U P T O 4 5 X 4 5 X 3 0 MM, ALL ARE TAN, RED AND SOFT; ALL EXUDE A TAN CREAMY SEMIFLUID; PORTIONS COLLECTED; REGIONAL LN (SEE INGUINAL) (AXILLARY) NORMAL (CAP 23) LN, INGUINAL (IN) : -LARGE; LEFT: UP TO 7 X 5 X 5 MM; APPEARS CYSTIC (CAP 22); RIGHT NOT IDENTIFIED LN, MANDIBULAR (MN) : -MOTTLED; BILATERAL: RED AND TAN (CAP 21) MAMMARY, FEMALE (MF) : >NOTE:>CARCINOMA FOUND IN MASS #1, PART OF MASS #2, AND ON THE SLIDE THAT GROSSLY WAS THOUGHT TO CONTAIN LYMPH NODE. MAJORITY OF MASS #2 IS FIBROADENOMA. THE CARCINOMA ON SLIDE #22 HAS CONSIDERABLE SQUAMOUS METAPLASIA. HEMATO NEOPLASIA (HN) : -M-SARCOMA, HISTIOCYTIC,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT LN, AXILLARY (AX) : -HEMORRHAGE,-PRESENT -HYPERPLASIA, LYMPHOPLASMACYTIC,-SLIGHT -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL LN, INGUINAL (IN) : >NOT REQUIRED TO BE EXAMINED FOR ANIMAL >NOTE:>SEE MAMMARY, FEMALE FOR DIAGNOSIS LN, MANDIBULAR (MN) : -HYPERPLASIA, LYMPHOPLASMACYTIC,MODERATE -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL 3272 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1514 ANIMAL NUMBER: C92992 SEX: FEMALE DATE OF DEATH: 11/23/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 11/23/99 15:21 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 583 STUDY WEEK OF DEATH: 84 TERMINAL BODY WEIGHT: 372.2 GRAMS PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, OTHER (LN), LN, RENAL (RL), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOt /f OOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA) 3273 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1515 ANIMAL NUMBER: C92993 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 271.2 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 13:54 PROSECTOR: STEVE VAN ADESTINE RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) LF ADRENAL (AD0) RT ADRENAL (AD1) BRAIN (BR) LF KIDNEY (KD0) RT KIDNEY (KD1) LIVER (LI) LUNG (LU) LF OVARY (OV0) RT OVARY (OV1) SPLEEN (SP) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) UTERUS (UT) .0384 .0376 1.9653 1.1048 1.1773 7.6933 1.1907 .0548 .0542 .5039 .0103 .0083 1.0577 .0142 % .0139 % .7247 % .4074 % .4341 % 2.8368 % .4390 % .0202 % .0200 % .1858 % .0038 % .0031 % .3900 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0195 .0191 1.0000 .5622 .5990 3.9146 .6059 .0279 .0276 .2564 .0052 .0042 .5382 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -NECROSIS, INDIVIDUAL HEPATOCYTE,MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-SLIGHT -NECROSIS, COAGULATIVE,-SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-MINIMAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 3274 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1516 ANIMAL NUMBER: C92993 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 271.2 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 13:54 PROSECTOR: STEVE VAN ADESTINE RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV) 3275 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1517 ANIMAL NUMBER: C92994 SEX: FEMALE DATE OF DEATH: 11/12/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 11/21/99 7:03 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 572 STUDY WEEK OF DEATH: 82 TERMINAL BODY WEIGHT: 239.6 GRAMS PROSECTOR: CHANESTA ACKER RECORDER: JOHN S. HALFORD PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >EYES; RED DISCHARGE; EYE-LEFT; VERIFIED AT NECROPSY: OBSERVED >EYES; DISCOLORED/LIGHT RED; EYE-LEFT; VERIFIED AT NECROPSY: NOT OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; YELLOW HAIR COAT; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; NO FECES; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) HISTOPATHOLOGY EYE (EY) : -DYSTROPHY, CORNEAL IN EARLY STAGES, CONSISTS PRIMARILY OF FOCAL MINERALIZATION,-MINIMAL -INFLAMMATION, ACUTE,-SLIGHT BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -DILATATION, PELVIC,-MINIMAL -MINERALIZATION, TUBULAR,-MINIMAL SPLEEN (SP) : -DEPLETION, LYMPHOCYTIC,-SLIGHT PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-SLIGHT -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT 3276 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1518 ANIMAL NUMBER: C92994 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/12/99 STUDY DAY OF DEATH: 572 STUDY WEEK OF DEATH: 82 TERMINAL BODY WEIGHT: 239.6 GRAMS DATE AND TIME OF NECROPSY: 11/21/99 7:03 PROSECTOR: CHANESTA ACKER RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PANCREAS (PA) : -MASS(ES); ATTACHED TO MID PORTION OF DUODENUM: SINGLE MASS, 33 X 17 X 12 MM, DARK RED, TAN, LOBULATED AND FIRM; SAME ON CUT SURFACE (CAP 22); REGIONAL LN (PANCREATIC) NOT IDENTIFIED THYMUS (TH) : -MISSING/NOT IDENTIFIED; NOT POSITIVELY IDENTIFIED; POSSIBLE REMNANTS COLLECTED PITUITARY (PI) : -MASS(ES); 8 X 6 X 4 MM, DARK RED, TAN, GREY AND FIRM ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-SLIGHT -ANGIECTASIS,-MINIMAL -HYPERTROPHY, CORTICAL CELL,-SLIGHT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATELY SEVERE HEART (HT) : -CARDIOMYOPATHY,-MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-SLIGHT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT STOMACH, GL (ST) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT RECTUM (RE) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT 3277 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1519 ANIMAL NUMBER: C92994 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/12/99 STUDY DAY OF DEATH: 572 STUDY WEEK OF DEATH: 82 TERMINAL BODY WEIGHT: 239.6 GRAMS DATE AND TIME OF NECROPSY: 11/21/99 7:03 PROSECTOR: CHANESTA ACKER RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY RECTUM (RE) : -MASS(ES); ATTACHED TO SEROSAL SURFACE: SINGLE MASS, 11 X 11 X 9 MM, RED, DARK RED, TAN AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (SEE ILIAC) VAGINA (VA) : -SEMIFLUID MATERIAL; WITHIN LUMEN: LIGHT GREEN, CREAMY SEMIFLUID MATERIAL LN, MEDIASTINAL (ML) : -LARGE; UP TO 13 X 9 X 6 MM (CAP 21) -DIFFUSELY RED; DARK RED LN, LUMBAR/ILIAC (LM) : -LARGE; LEFT ILIAC: UP TO 7 X 5 X 2 MM (RIGHT ILIAC NOT IDENTIFIED) (CAP 23) -DIFFUSELY RED; LEFT: DARK RED -MASS(ES); BILATERAL, LUMBAR INVOLVING ENTIRE ORGAN: SINGLE MASS, 40 X 25 X 20 MM, RED, DARK RED, TAN, LOBULATED AND FIRM; SAME ON CUT SURFACE (CAP 25) LN, POPLITEAL (PP) : -LARGE; LEFT: 7 X 5 X 3 MM (RIGHT NOT IDENTIFIED) (CAP 24) MAMMARY, FEMALE (MF) : -HYPERPLASIA, GLANDULAR,-MINIMAL VAGINA (VA) : -LUMEN, EXUDATE,-PRESENT HEMATO NEOPLASIA (HN) : -M-LYMPHOMA,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, HEMATOPOIETIC,-PRESENT LN, MEDIASTINAL (ML) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT LN, LUMBAR/ILIAC (LM) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT LN, POPLITEAL (PP) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT 3278 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1520 ANIMAL NUMBER: C92994 SEX: FEMALE DATE OF DEATH: 11/12/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 11/21/99 7:03 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 572 STUDY WEEK OF DEATH: 82 TERMINAL BODY WEIGHT: 239.6 GRAMS PROSECTOR: CHANESTA ACKER RECORDER: JOHN S. HALFORD PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY LN, POPLITEAL (PP) : -DIFFUSELY RED; LEFT: DARK RED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DRY, MATERIAL -ANIMAL THIN RED 3279 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1521 ANIMAL NUMBER: C92994 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/12/99 STUDY DAY OF DEATH: 572 STUDY WEEK OF DEATH: 82 TERMINAL BODY WEIGHT: 239.6 GRAMS DATE AND TIME OF NECROPSY: 11/21/99 7:03 PROSECTOR: CHANESTA ACKER RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV) 3280 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1522 ANIMAL NUMBER: C92995 SEX: FEMALE DATE OF DEATH: 06/17/98 STUDY DAY OF DATE AND TIME OF NECROPSY: 06/17/98 10:39 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 59 STUDY WEEK OF DEATH: 9 TERMINAL BODY WEIGHT: 264.0 GRAMS PROSECTOR: TROY ELLENBECKER RECORDER: TERRI L. WILLIAMS PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; SORE/SCAB; TAIL; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; SWOLLEN; TAIL-DISTAL; VERIFIED AT NECROPSY: NOT OBSERVED >APPEARANCE; MISSING; TAIL-DISTAL; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; PALE; ENTIRE BODY; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY TAIL (TI) : -ABRASION(S)/ULCERATION(S); 120 MM FROM BASE: MULTIPLE RED, TAN AND MOIST ABRADED AREAS, UP TO 7 X 6 MM; COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -TAIL - BENT/MISSING; 120 MM FROM BASE: TIP MISSING; REMAINING PORTION COLLECTED HISTOPATHOLOGY LIVER (LI) : -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL KIDNEY (KD) : -ATROPHY, TUBULAR,-MINIMAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATELY SEVERE UTERUS (UT) : -DILATATION,-SLIGHT CAUSE OF DEATH (CD) : -INFLAMMATION / INFECTION,-PRESENT TAIL (TI) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE -ULCERATION,-SLIGHT 3281 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1523 ANIMAL NUMBER: C92995 SEX: FEMALE DATE OF DEATH: 06/17/98 STUDY DAY OF DATE AND TIME OF NECROPSY: 06/17/98 10:39 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 59 STUDY WEEK OF DEATH: 9 TERMINAL BODY WEIGHT: 264.0 GRAMS PROSECTOR: TROY ELLENBECKER RECORDER: TERRI L. WILLIAMS PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 3282 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1524 ANIMAL NUMBER: C92996 SEX: FEMALE DATE OF DEATH: 05/15/98 STUDY DAY OF DATE AND TIME OF NECROPSY: 05/15/98 11:01 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 200.3 GRAMS PROSECTOR: JODI THIEREN RECORDER: DEBBIE PIRKEL PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN STATUS LF ADRENAL (AD0) RT ADRENAL (AD1) BRAIN (BR) LF KIDNEY (KD0) RT KIDNEY (KD1) LIVER (LI) LUNG (LU) LF OVARY (OV0) RT OVARY (OV1) SPLEEN (SP) LF THYROID/PARA RT THYROID/PARA UTERUS (UT) (TT0) (TT1) -------- 7.1652 -------- -------- -------- -------- 3.5765 % -------- -------- -------- -------- --------------- -------- -------- NOT TAKEN WEIGHT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS NOTTAK NOTTAK NOTTAK NOTTAK NOTTAK NOTTAK NOTTAK NOTTAK 3283 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1525 ANIMAL NUMBER: C92996 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 200.3 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 11:01 PROSECTOR: JODI THIEREN RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) 3284 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1526 ANIMAL NUMBER: C92997 SEX: FEMALE DATE OF DEATH: 11/15/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 11/15/99 13:23 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 575 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: 307.6 GRAMS PROSECTOR: JILL PAUS RECORDER: JILL PAUS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); VENTRAL-ABDOMINAL-RIGHT; WEEK 74; 30 X 30MM; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; RECUMBENT; LATERAL; VERIFIED AT NECROPSY: NOT APPLICABLE >EYES; RED DISCHARGE; EYES; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) ADRENAL, CORTEX (AC) : -MOTTLED; BILATERAL: RED AND BROWN THYMUS (TH) : -RED FOCUS(I)/AREA(S); DIFFUSE: MULTIPLE DARK RED AREAS, PINPOINT AND UP TO 1 MM IN DIAMETER HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL -MINERALIZATION, TUBULAR,-MINIMAL -INFLAMMATION, SUPPURATIVE, PELVIC,MODERATE SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE PANCREAS (PA) : -ATROPHY, ACINAR,-MINIMAL ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC / HEMORRHAGIC,MODERATELY SEVERE MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,-SLIGHT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MINIMAL 3285 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1527 ANIMAL NUMBER: C92997 SEX: FEMALE DATE OF DEATH: 11/15/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 11/15/99 13:23 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 575 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: 307.6 GRAMS PROSECTOR: JILL PAUS RECORDER: JILL PAUS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 11 X 5 X 5 MM, RED, DARK RED, TAN AND SOFT MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT INGUINAL REGION: SINGLE MASS, 40 X 20 X 20 MM, RED, TAN, FIRM AND LOBULATED; ON SECTION, EXUDES A TAN CREAMY SEMIFLUID; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: RED, DRY MATERIAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -B-FIBROADENOMA,-NEOPLASM PRESENT -HYPERPLASIA, GLANDULAR,-SLIGHT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 3286 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1528 ANIMAL NUMBER: C92997 SEX: FEMALE DATE OF DEATH: 11/15/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 11/15/99 13:23 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 575 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: 307.6 GRAMS PROSECTOR: JILL PAUS RECORDER: JILL PAUS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 3287 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1529 ANIMAL NUMBER: C92998 SEX: FEMALE DATE OF DEATH: 03/01/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 03/01/99 15:00 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 316 STUDY WEEK OF DEATH: 46 TERMINAL BODY WEIGHT: 205.8 GRAMS PROSECTOR: TERRI L. WILLIAMS RECORDER: KEVIN BILLINGS PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >EYES; SQUINTED; PERI-ORBITAL; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; YELLOW HAIR COAT; VERIFIED AT NECROPSY: NOT OBSERVED >BEHAVIOR; ATAXIC; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -DIFFUSELY DARK; DIAPHRAGMATIC SURFACE OF LEFT LATERAL LOBE: SINGLE BROWN FOCI 2 MM IN DIAMETER HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -HYPERPLASIA, BILE DUCT,-MINIMAL -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL -PIGMENT, TUBULAR,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE MARROW, FEMUR (FM) : -CONGESTION,-PRESENT MARROW, STERNUM (SE) : -CONGESTION,-PRESENT SPINAL CORD (SC) : -AXONAL SWELLING,-MODERATE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATE LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL 3288 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1530 ANIMAL NUMBER: C92998 SEX: FEMALE DATE OF DEATH: 03/01/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 03/01/99 15:00 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 316 STUDY WEEK OF DEATH: 46 TERMINAL BODY WEIGHT: 205.8 GRAMS PROSECTOR: TERRI L. WILLIAMS RECORDER: KEVIN BILLINGS PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 8 MM IN DIAMETER, DARK RED, GREY AND SOFT MAMMARY, FEMALE (MF) : -THICKENED; ALL REGIONS DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN -STAINS-PERINEUM/PERIANAL; YELLOW MATERIAL MOIST PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT -ANGIECTASIS,-PRESENT MAMMARY, FEMALE (MF) : -HYPERPLASIA, GLANDULAR,-MODERATE VAGINA (VA) : -LUMEN, EXUDATE,-PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 3289 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1531 ANIMAL NUMBER: C92998 SEX: FEMALE DATE OF DEATH: 03/01/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 03/01/99 15:00 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 316 STUDY WEEK OF DEATH: 46 TERMINAL BODY WEIGHT: 205.8 GRAMS PROSECTOR: TERRI L. WILLIAMS RECORDER: KEVIN BILLINGS PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), BONE, STERNUM (SB), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), HEMATO NEOPLASIA (HN) 3290 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1532 ANIMAL NUMBER: C92999 SEX: FEMALE DATE OF DEATH: 01/22/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 01/22/00 15:02 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DEATH: 643 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: NOT ENTERED PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); VENTRAL-ABDOMINAL; WEEK 59; 40 X 40MM; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; HEAD TILT; LEFT; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) KIDNEY (KD) : -LARGE PELVIS(ES); BILATERAL HISTOPATHOLOGY EYE (EY) : -DYSTROPHY, CORNEAL IN EARLY STAGES, CONSISTS PRIMARILY OF FOCAL MINERALIZATION,-MINIMAL BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -DILATATION, PELVIC,-MODERATELY SEVERE -MINERALIZATION, TUBULAR,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MINIMAL -HYPERTROPHY, CORTICAL CELL,-MODERATE -PIGMENT,-SLIGHT OVARY (OV) : -CYST,-PRESENT 3291 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1533 ANIMAL NUMBER: C92999 SEX: FEMALE DATE OF DEATH: 01/22/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 01/22/00 15:02 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DEATH: 643 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: NOT ENTERED PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 10 X 7 X 4 MM, DARK RED AND SOFT MAMMARY, FEMALE (MF) : -MASS(ES); LEFT INGUINAL REGION: SINGLE MASS, 45 X 35 X 16 MM, TAN, GREEN, VASCULAR AND SOFT; ON SECTION EXUDES A GREEN CREAMY SEMIFLUID; COLLECTED INTACT; REGIONAL LN (INGUINAL) NOT IDENTIFIED; POSSIBLY CONSISTENT WITH MASS (VENTRAL ABDOMINAL) NOTED IN CLINICAL OBSERVATION MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,MODERATELY SEVERE MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,MODERATELY SEVERE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MINIMAL HEART (HT) : -CARDIOMYOPATHY,-SLIGHT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT JEJUNUM (JE) : >AUTOLYTIC MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT -INFLAMMATION, CHRONIC-ACTIVE,MODERATELY SEVERE -B-ADENOMA,-NEOPLASM PRESENT UTERUS (UT) : -DILATATION,-SLIGHT 3292 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1534 ANIMAL NUMBER: C92999 SEX: FEMALE DATE OF DEATH: 01/22/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 01/22/00 15:02 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DEATH: 643 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: NOT ENTERED PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL: DARK RED -LARGE; BILATERAL: EACH IS 10 X 7 X 2 MM; COLLECTED GENERAL COMMENT (GC) : -ANIMAL THIN CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT LN, MANDIBULAR (MN) : -HYPERPLASIA, LYMPHOPLASMACYTIC,MODERATELY SEVERE 3293 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1535 ANIMAL NUMBER: C92999 SEX: FEMALE DATE OF DEATH: 01/22/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 01/22/00 15:02 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DEATH: 643 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: NOT ENTERED PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), TAIL (TI), LN, LUMBAr /i LIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 3294 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1536 ANIMAL NUMBER: C93000 SEX: FEMALE DATE OF DEATH: 07/21/98 STUDY DAY OF DATE AND TIME OF NECROPSY: 07/21/98 13:56 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 267.6 GRAMS PROSECTOR: MICHELE MARGGI RECORDER: MATTHEW AXTMAN PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: MATTHEW AXTMAN ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) LF ADRENAL (AD0) RT ADRENAL (AD1) BRAIN (BR) LF KIDNEY (KD0) RT KIDNEY (KD1) LIVER (LI) LUNG (LU) LF OVARY (OV0) RT OVARY (OV1) SPLEEN (SP) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) UTERUS (UT) .0366 .0430 2.0797 1.1525 1.1261 9.1952 1.4706 .0643 .0895 .4800 .0115 .0225 .6052 .0137 % .0161 % .7772 % .4307 % .4208 % 3.4362 % .5496 % .0240 % .0334 % .1794 % .0043 % .0084 % .2262 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0176 .0207 1.0000 .5542 .5415 4.4214 .7071 .0309 .0430 .2308 .0055 .0108 .2910 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -NECROSIS, INDIVIDUAL HEPATOCYTE,MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 3295 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1537 ANIMAL NUMBER: C93000 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 267.6 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 13:56 PROSECTOR: MICHELE MARGGI RECORDER: MATTHEW AXTMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: MATTHEW AXTMAN THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV) 3296 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1538 ANIMAL NUMBER: C93001 SEX: FEMALE DATE OF DEATH: 11/24/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 11/24/99 7:18 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 584 STUDY WEEK OF DEATH: 84 TERMINAL BODY WEIGHT: 321.6 GRAMS PROSECTOR: TODD ACKER RECORDER: TODD ACKER PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK 76; 30 X 30MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); INGUINAL REGION-LEFT; WEEK 82; 10 X 10MM; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; HEAD TILT; RIGHT; VERIFIED AT NECROPSY: NOT APPLICABLE >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; ATAXIC; VERIFIED AT NECROPSY: NOT APPLICABLE >EYES; RED DISCHARGE; EYES; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) HISTOPATHOLOGY BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, PELVIC,-SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-MODERATE -DEGENERATION, CYSTIC / HEMORRHAGIC,SLIGHT -HYPERPLASIA, CORTICAL,-SLIGHT -PIGMENT,-SLIGHT ADRENAL, MEDULLA (MA) : -B-PHEOCHROMOCYTOMA,-NEOPLASM PRESENT MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,-SLIGHT 3297 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1539 ANIMAL NUMBER: C93001 SEX: FEMALE DATE OF DEATH: 11/24/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 11/24/99 7:18 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 584 STUDY WEEK OF DEATH: 84 TERMINAL BODY WEIGHT: 321.6 GRAMS PROSECTOR: TODD ACKER RECORDER: TODD ACKER PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 12 X 10 X 6 MM, DARK RED, TAN AND SOFT MAMMARY, FEMALE (MF) : -MASS(ES); LEFT INGUINAL REGION: SINGLE MASS, 17 X 15 X 8 MM, TAN, RED AND SOFT; CUT SURFACE EXUDES A TAN, CREAMY SEMIFLUID (CAP 21); REGIONAL LN (INGUINAL) NOT IDENTIFIED -MASS #2; LEFT PERIANAL REGION: SINGLE MASS, 35 X 32 X 20 MM, DARK RED, TAN, YELLOW AND SOFT; CUT SURFACE EXUDES A DARK RED SEMIFLUID; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,-SLIGHT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY), MINIMAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PARATHYROID (PT) : >SECTION EXAMINED; TISSUE NOT PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -M-CARCINOMA,-NEOPLASM PRESENT -CYST / GALACTOCELE,-PRESENT -B-FIBROADENOMA,-NEOPLASM PRESENT >NOTE:>MASS #1 IS THE FIBROADENOMA UTERUS (UT) : -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL VAGINA (VA) : -HYPERPLASIA, MUCINOUS,-MINIMAL 3298 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1540 ANIMAL NUMBER: C93001 SEX: FEMALE DATE OF DEATH: 11/24/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 11/24/99 7:18 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 584 STUDY WEEK OF DEATH: 84 TERMINAL BODY WEIGHT: 321.6 GRAMS PROSECTOR: TODD ACKER RECORDER: TODD ACKER PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DARK RED AND DRY CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), HEMATO NEOPLASIA (HN) 3299 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1541 ANIMAL NUMBER: C93002 SEX: FEMALE DATE OF DEATH: 11/01/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 11/01/99 14:15 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 561 STUDY WEEK OF DEATH: 81 TERMINAL BODY WEIGHT: 381.4 GRAMS PROSECTOR: JOHN S. HALFORD RECORDER: DEBBIE PIRKEL PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); INGUINAL REGION-RIGHT; WEEK 56; 70 X 55MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); AXILLARY REGION-LEFT; WEEK 70; 20 X 20MM; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >RESPIRATION; IRREGULAR; VERIFIED AT NECROPSY: NOT APPLICABLE >EYES; RED DISCHARGE; EYES; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; RED HAIR COAT; HEADENTIRE; VERIFIED AT NECROPSY: OBSERVED >RESPIRATION; LABORED; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) OVARY (OV) : -CYST(S); LEFT: SINGLE CLEAR FLUID FILLED CYST, 3 MM IN DIAMETER HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL KIDNEY (KD) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -MINERALIZATION, PELVIC,-MINIMAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SLIGHT PANCREAS (PA) : -ATROPHY, ACINAR,-MINIMAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT -HYPERTROPHY, CORTICAL CELL,-MINIMAL -PIGMENT,-SLIGHT OVARY (OV) : -CYST,-PRESENT 3300 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1542 ANIMAL NUMBER: C93002 SEX: FEMALE DATE OF DEATH: 11/01/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 11/01/99 14:15 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 561 STUDY WEEK OF DEATH: 81 TERMINAL BODY WEIGHT: 381.4 GRAMS PROSECTOR: JOHN S. HALFORD RECORDER: DEBBIE PIRKEL PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY THYMUS (TH) : -MISSING/NOT IDENTIFIED; DUE TO LUNG MASS; THYMUS NOT POSITIVELY IDENTIFIED; POSSIBLE REMNANTS COLLECTED LUNG (LU) : -MASS(ES); INVOLVING ENTIRE RIGHT CAUDAL AND MIDDLE LOBES: SINGLE MASS, 48 X 23 X 18 MM, RED, DARK RED, GRAY, TAN, LOBULATED, VASCULAR, AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (TRACHEOBRONCHIAL) NORMAL (CAP 22); PORTION OF MASS ADHERED TO DIAPHRAGM; ADHESIONS COLLECTED WITH MASS PITUITARY (PI) : -MASS(ES); 12 X 11 X 8 MM, DARK RED, LOBULATED, AND SOFT MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,MODERATELY SEVERE MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,MODERATELY SEVERE THYMUS (TH) : -M-THYMIC CARCINOMA,-NEOPLASM PRESENT LUNG (LU) : >UNREMARKABLE >NOTE:>SEE THYMUS FOR DIAGNOSIS OF MASS HEART (HT) : -CARDIOMYOPATHY,-MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-SLIGHT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT 3301 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1543 ANIMAL NUMBER: C93002 SEX: FEMALE DATE OF DEATH: 11/01/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 11/01/99 14:15 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 561 STUDY WEEK OF DEATH: 81 TERMINAL BODY WEIGHT: 381.4 GRAMS PROSECTOR: JOHN S. HALFORD RECORDER: DEBBIE PIRKEL PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS(ES); LEFT AXILLARY REGION: SINGLE MASS, 48 X 30 X 18 MM, RED, DARK RED, LOBULATED, AND SOFT; ON SECTION EXUDES A BROWN FLUID (CAP 23); REGIONAL LN (AXILLARY) NORMAL (CAP 24) -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION -MASS #2; RIGHT INGUINAL REGION: SINGLE MASS, 55 X 40 X 40 MM, RED, DARK RED, BLACK, GRAY, TAN, VASCULAR, LOBULATED, AND SOFT; ON SECTION EXUDES A BROWN FLUID; COLLECTED INTACT; REGIONAL LN (INGUINAL) NOT IDENTIFIED UTERUS (UT) : -CYST(S); RIGHT, WITHIN WALLS: MULTIPLE CLEAR FLUID FILLED CYSTS, UP TO 3 MM IN DIAMETER VAGINA (VA) : -SEMIFLUID MATERIAL; WITHIN LUMEN: GREEN MUCOID SEMIFLUID MATERIAL LN, MANDIBULAR (MN) : -LARGE; LEFT: UP TO 8 X 5 X 3 MM (RIGHT IS UP TO 4 X 4 X 3 MM) (CAP 21) MAMMARY, FEMALE (MF) : -M-CARCINOMA,- 2 NEOPLASMS PRESENT -CYST / GALACTOCELE,-PRESENT -HYPERPLASIA, GLANDULAR,-SLIGHT UTERUS (UT) : -DILATATION,-MODERATELY SEVERE VAGINA (VA) : -LUMEN, EXUDATE,-PRESENT -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT LN, AXILLARY (AX) : -HYPERPLASIA, LYMPHOPLASMACYTIC,-SLIGHT LN, MANDIBULAR (MN) : -HYPERPLASIA, LYMPHOPLASMACYTIC,MODERATELY SEVERE 3302 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1544 ANIMAL NUMBER: C93002 SEX: FEMALE DATE OF DEATH: 11/01/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 11/01/99 14:15 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 561 STUDY WEEK OF DEATH: 81 TERMINAL BODY WEIGHT: 381.4 GRAMS PROSECTOR: JOHN S. HALFORD RECORDER: DEBBIE PIRKEL PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; RIGHT: DRY, DARK RED MATERIAL -ANIMAL THIN -DISCHARGE-NASAL; DARK RED AND DRY; POSSIBLY CONSISTENT WITH (RED HAIRCOAT ENTIRE HEAD) NOTED IN CLINICAL OBSERVATIONS GENERAL INFORMATION (XX) : >NOTE:>OCULAR DISCHARGE (LEFT EYE) NOTED IN CLINICAL OBSERVATIONS WAS NOT SEEN AT NECROPSY LN, TRACHEOBRON (TB) : -HYPERPLASIA, LYMPHOPLASMACYTIC,MINIMAL 3303 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1545 ANIMAL NUMBER: C93002 SEX: FEMALE DATE OF DEATH: 11/01/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 11/01/99 14:15 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 561 STUDY WEEK OF DEATH: 81 TERMINAL BODY WEIGHT: 381.4 GRAMS PROSECTOR: JOHN S. HALFORD RECORDER: DEBBIE PIRKEL PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), HEMATO NEOPLASIA (HN) 3304 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1546 ANIMAL NUMBER: C93003 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/07/00 STUDY DAY OF DEATH: 719 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 625.8 GRAMS DATE AND TIME OF NECROPSY: 04/07/00 7:24 PROSECTOR: JAMES T. CALLEN RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: NOT OBSERVED >MASS(ES); AXILLARY REGION-LEFT; WEEK 56; 90 X 90MM; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; AXILLARY REGION-LEFT; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; YELLOW HAIR COAT; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE DARK RED AREAS, UP TO 4 X 3 MM, SOME ARE SUNKEN WITH A STELLATE PATTERN; COLLECTED ON ROUTINE SECTIONS OVARY (OV) : -CYST(S); LEFT: SINGLE CLEAR FLUID FILLED CYST, 3 MM IN DIAMETER HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -NECROSIS, INDIVIDUAL HEPATOCYTE,MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-SLIGHT -NECROSIS, COAGULATIVE,-SLIGHT -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL -ANGIECTASIS,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, TUBULAR,-MINIMAL OVARY (OV) : >UNREMARKABLE LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL 3305 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1547 ANIMAL NUMBER: C93003 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/07/00 STUDY DAY OF DEATH: 719 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 625.8 GRAMS DATE AND TIME OF NECROPSY: 04/07/00 7:24 PROSECTOR: JAMES T. CALLEN RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS(ES); LEFT AXILLARY REGION: SINGLE MASS, 105 X 105 X 45 MM, RED, TAN, LOBULATED, AND SOFT; SAME ON CUT SURFACE; OVERLYING SKIN HAS A MOIST RED ULCERATED AREA, 35 X 30 MM; COLLECTED INTACT WITH MASS; REPRESENTATIVE PORTIONS COLLECTED; REGIONAL LN (AXILLARY) NORMAL (CAP 22) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -STAINS-PERINEUM/PERIANAL; MATERIAL DRY YELLOW THYROID (TY) : -COLLOID, DECREASED,-SEVERE MAMMARY, FEMALE (MF) : -B-FIBROADENOMA,-NEOPLASM PRESENT -HYPERPLASIA, GLANDULAR,-SLIGHT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, AXILLARY (AX) : -HYPERPLASIA, LYMPHOPLASMACYTIC,MODERATE 3306 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1548 ANIMAL NUMBER: C93003 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/07/00 STUDY DAY OF DEATH: 719 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 625.8 GRAMS DATE AND TIME OF NECROPSY: 04/07/00 7:24 PROSECTOR: JAMES T. CALLEN RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), PARATHYROID (PT), URINARY BLADDER (UB) 3307 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1549 ANIMAL NUMBER: C93004 SEX: FEMALE DATE OF DEATH: 09/21/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 09/21/99 14:10 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 520 STUDY WEEK OF DEATH: 75 TERMINAL BODY WEIGHT: 322.9 GRAMS PROSECTOR: JARED FISHEL RECORDER: DENNIS HOFFMAN PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >EYES; SQUINTED; PERI-ORBITAL-RIGHT; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; ATAXIC; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) ADRENAL, CORTEX (AC) : -LARGE; LEFT: 8 MM IN DIAMETER (RIGHT IS 4 X 3 X 3 MM) OVARY (OV) : -CYST(S); LEFT: SINGLE CLEAR FLUID FILLED CYST, 3 MM IN DIAMETER HISTOPATHOLOGY BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC / HEMORRHAGIC,MODERATELY SEVERE OVARY (OV) : -CYST,-PRESENT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),SLIGHT LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL 3308 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1550 ANIMAL NUMBER: C93004 SEX: FEMALE DATE OF DEATH: 09/21/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 09/21/99 14:10 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 520 STUDY WEEK OF DEATH: 75 TERMINAL BODY WEIGHT: 322.9 GRAMS PROSECTOR: JARED FISHEL RECORDER: DENNIS HOFFMAN PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); SINGLE MASS, 10 X 9 X 9 MM, RED, DARK RED, TAN, LOBULATED AND SOFT LN, MANDIBULAR (MN) : -MOTTLED; BILATERAL: RED AND TAN (CAP 21) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DRY RED MATERIAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT -HYPERPLASIA, GLANDULAR,-SLIGHT VAGINA (VA) : -LUMEN, EXUDATE,-PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT LN, MANDIBULAR (MN) : -HEMORRHAGE,-PRESENT 3309 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1551 ANIMAL NUMBER: C93004 SEX: FEMALE DATE OF DEATH: 09/21/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 09/21/99 14:10 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 520 STUDY WEEK OF DEATH: 75 TERMINAL BODY WEIGHT: 322.9 GRAMS PROSECTOR: JARED FISHEL RECORDER: DENNIS HOFFMAN PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), KIDNEY (KD), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), HEMATO NEOPLASIA (HN) 3310 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1552 ANIMAL NUMBER: C93005 SEX: FEMALE DATE OF DEATH: 01/09/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 01/09/00 9:01 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 630 STUDY WEEK OF DEATH: 90 TERMINAL BODY WEIGHT: 327.1 GRAMS PROSECTOR: DONIA MEYER RECORDER: DONIA MEYER PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); AXILLARY REGION-LEFT; WEEK 51; 40 X 45MM; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: NOT OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >EYES; RED DISCHARGE; EYES; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; ATAXIC; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) HISTOPATHOLOGY BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, PELVIC,-SLIGHT -HYPERPLASIA, TRANSITIONAL CELL,-SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC / HEMORRHAGIC,MODERATELY SEVERE -PIGMENT,-MINIMAL LN, MESENTERIC (MS) : -HYPERPLASIA, LYMPHORETICULAR,-SEVERE MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,-MODERATE 3311 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1553 ANIMAL NUMBER: C93005 SEX: FEMALE DATE OF DEATH: 01/09/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 01/09/00 9:01 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 630 STUDY WEEK OF DEATH: 90 TERMINAL BODY WEIGHT: 327.1 GRAMS PROSECTOR: DONIA MEYER RECORDER: DONIA MEYER PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 14 X 10 X 9 MM, RED, DARK RED, GRAY AND SOFT MAMMARY, FEMALE (MF) : -MASS(ES); LEFT AXILLARY REGION: SINGLE MASS, 55 X 50 X 30 MM, RED, DARK RED, TAN, FIRM AND LOBULATED; SOME SECTIONS EXUDE A TAN, CREAMY SEMISOLID; COLLECTED; REGIONAL LN (AXILLARY) NORMAL (CAP 21) -CYST(S); ALL REGIONS, DIFFUSE: MULTIPLE TAN AND CREAMY SEMIFLUID FILLED CYSTS, UP TO 5 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION THYMUS (TH) : -HYPERPLASIA, LYMPHORETICULAR,-SEVERE PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, NONGL (SU) : -ACANTHOSIS / HYPERKERATOSIS,-SLIGHT -EDEMA,-PRESENT -INFLAMMATION, SUBACUTE,-MODERATE SKIN (SK) : -M-FIBROSARCOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT -B-FIBROADENOMA,-NEOPLASM PRESENT -HYPERPLASIA, GLANDULAR,-SLIGHT -B-ADENOMA,-NEOPLASM PRESENT >NOTE:>SEE ALSO SKIN, AS MASS CONTAINS TWO DISTINCT COMPONENTS. MASS 1 IS THE FIBROADENOMA VAGINA (VA) : -HYPERPLASIA, MUCINOUS,-MINIMAL -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 3312 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1554 ANIMAL NUMBER: C93005 SEX: FEMALE DATE OF DEATH: 01/09/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 01/09/00 9:01 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 630 STUDY WEEK OF DEATH: 90 TERMINAL BODY WEIGHT: 327.1 GRAMS PROSECTOR: DONIA MEYER RECORDER: DONIA MEYER PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DRY RED MATERIAL LN, AXILLARY (AX) : -HYPERPLASIA, RETICULOENDOTHELIAL,SLIGHT 3313 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1555 ANIMAL NUMBER: C93005 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/09/00 STUDY DAY OF DEATH: 630 STUDY WEEK OF DEATH: 90 TERMINAL BODY WEIGHT: 327.1 GRAMS DATE AND TIME OF NECROPSY: 01/09/00 9:01 PROSECTOR: DONIA MEYER RECORDER: DONIA MEYER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER UTERUS (UT), CERVIX (CV), HEMATO NEOPLASIA (HN) (UB), 3314 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1556 ANIMAL NUMBER: C93006 SEX: FEMALE DATE OF DEATH: 05/15/98 STUDY DAY OF DATE AND TIME OF NECROPSY: 05/15/98 11:39 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 193.9 GRAMS PROSECTOR: JODI THIEREN RECORDER: DEBBIE PIRKEL PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN STATUS LF ADRENAL (AD0) RT ADRENAL (AD1) BRAIN (BR) LF KIDNEY (KD0) RT KIDNEY (KD1) LIVER (LI) LUNG (LU) LF OVARY (OV0) RT OVARY (OV1) SPLEEN (SP) LF THYROID/PARA RT THYROID/PARA UTERUS (UT) (TT0) (TT1) -------- 6.9154 -------- -------- -------- -------- 3.5657 % -------- -------- -------- -------- --------------- -------- -------- NOT TAKEN WEIGHT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS NOTTAK NOTTAK NOTTAK NOTTAK NOTTAK NOTTAK NOTTAK NOTTAK 3315 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1557 ANIMAL NUMBER: C93006 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 193.9 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 11:39 PROSECTOR: JODI THIEREN RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) 3316 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1558 ANIMAL NUMBER: C93007 SEX: FEMALE DATE OF DEATH: 04/07/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/07/00 7:23 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 719 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 510.2 GRAMS PROSECTOR: MICHELE MARGGI RECORDER: JILL PAUS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY PITUITARY (PI) : -LARGE; 6 X 5 X 4 MM; APPEARS SWOLLEN SKIN (SK) : -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION CERVIX (CV) : -LARGE; 11 X 10 X 5 MM; FIRM GENERAL COMMENT (GC) : -BLOOD FILM TAKEN HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-SLIGHT -HYPERPLASIA, BILE DUCT,-SLIGHT -FIBROSIS, PERIBILIARY,-MINIMAL LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-SLIGHT PITUITARY (PI) : -HYPERPLASIA,-MINIMAL SKIN (SK) : >NOT REQUIRED TO BE EXAMINED FOR ANIMAL MAMMARY, FEMALE (MF) : -B-FIBROADENOMA,-NEOPLASM PRESENT CERVIX (CV) : -HYPERPLASIA, MUCINOUS,-MODERATELY SEVERE CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 3317 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1559 ANIMAL NUMBER: C93007 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/07/00 STUDY DAY OF DEATH: 719 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 510.2 GRAMS DATE AND TIME OF NECROPSY: 04/07/00 7:23 PROSECTOR: MICHELE MARGGI RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: KIDNEY (KD), PANCREAS (PA), PARATHYROID (PT), URINARY BLADDER (UB) 3318 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1560 ANIMAL NUMBER: C93008 SEX: FEMALE DATE OF DEATH: 05/15/98 STUDY DAY OF DATE AND TIME OF NECROPSY: 05/15/98 11:38 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 211.9 GRAMS PROSECTOR: LYNN HORNER RECORDER: DEBBIE PIRKEL PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN STATUS LF ADRENAL (AD0) RT ADRENAL (AD1) BRAIN (BR) LF KIDNEY (KD0) RT KIDNEY (KD1) LIVER (LI) LUNG (LU) LF OVARY (OV0) RT OVARY (OV1) SPLEEN (SP) LF THYROID/PARA RT THYROID/PARA UTERUS (UT) (TT0) (TT1) -------- 7.8828 -------- -------- -------- -------- 3.7198 % -------- -------- -------- -------- --------------- -------- -------- NOT TAKEN WEIGHT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS NOTTAK NOTTAK NOTTAK NOTTAK NOTTAK NOTTAK NOTTAK NOTTAK 3319 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1561 ANIMAL NUMBER: C93008 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 211.9 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 11:38 PROSECTOR: LYNN HORNER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) 3320 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1562 ANIMAL NUMBER: C93009 SEX: FEMALE DATE OF DEATH: 01/21/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 01/21/00 15:07 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 642 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: 358.8 GRAMS PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); VENTRAL-ABDOMINAL-RIGHT; WEEK 79; 10 X 10MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); HEAD-MAXILLARY-LEFT; WEEK 91; 25 X 20MM; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED >EYES; SQUINTED; PERI-ORBITAL-RIGHT; VERIFIED AT NECROPSY: NOT APPLICABLE >EYES; DISCOLORED/LIGHT RED; EYE-LEFT; VERIFIED AT NECROPSY: NOT APPLICABLE >EYES; SCAB; EYE-LEFT; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY EYE (EY) : -ROUGHENED CORNEA; LEFT BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) HISTOPATHOLOGY EYE (EY) : -INFLAMMATION, CHRONIC-ACTIVE,-SEVERE -ULCERATION, CORNEAL,-MODERATE BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-MINIMAL -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL -MINERALIZATION, TUBULAR,-MINIMAL -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-MINIMAL -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL ADRENAL, CORTEX (AC) : -HYPERPLASIA, CORTICAL,-MINIMAL -PIGMENT,-SLIGHT 3321 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1563 ANIMAL NUMBER: C93009 SEX: FEMALE DATE OF DEATH: 01/21/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 01/21/00 15:07 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 642 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: 358.8 GRAMS PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 12 X 7 X 5 MM, DARK RED, TAN AND FIRM MAMMARY, FEMALE (MF) : -MASS(ES); LEFT INGUINAL REGION: SINGLE MASS, 25 X 19 X 12 MM, RED, DARK RED, TAN AND SOFT; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED LN, MANDIBULAR (MN) : -LARGE; BILATERAL: EACH IS 9 X 5 X 3 MM; COLLECTED MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,MODERATELY SEVERE LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL HEART (HT) : -CARDIOMYOPATHY,-MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL MAMMARY, FEMALE (MF) : -M-CARCINOMA,-NEOPLASM PRESENT -HYPERPLASIA, GLANDULAR,-SLIGHT VAGINA (VA) : -HYPERPLASIA, MUCINOUS,-SLIGHT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT LN, MANDIBULAR (MN) : -HYPERPLASIA, LYMPHOPLASMACYTIC,MODERATE AUDITORY SEB GL (AS) : -M-CARCINOMA,-NEOPLASM PRESENT 3322 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1564 ANIMAL NUMBER: C93009 SEX: FEMALE DATE OF DEATH: 01/21/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 01/21/00 15:07 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 642 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: 358.8 GRAMS PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY CALVARIUM/SKULL (CA) : -MASS(ES); LEFT SIDE OF HEAD, NEAR BASE OF EAR: SINGLE MASS, 25 X 24 X 18 MM, RED, TAN AND FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (SEE MANDIBULAR) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL, LOWER INCISORS CALVARIUM/SKULL (CA) : >NOT REQUIRED TO BE EXAMINED FOR ANIMAL >NOTE:>SEE AUDITORY SEBACEOUS GLAND FOR DIAGNOSIS 3323 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1565 ANIMAL NUMBER: C93009 SEX: FEMALE DATE OF DEATH: 01/21/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 01/21/00 15:07 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 642 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: 358.8 GRAMS PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), LN, ANT MEs /pANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), HEMATO NEOPLASIA (HN) 3324 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1566 ANIMAL NUMBER: C93010 SEX: FEMALE DATE OF DEATH: 01/10/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 01/10/00 15:04 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 631 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: 377.0 GRAMS PROSECTOR: WENDY CHERRY RECORDER: JODI THIEREN PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >EYES; RED DISCHARGE; EYES; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; BROWN HAIR COAT; PERINEAL AREA; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; HEAD TILT; RIGHT; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -MOTTLED; ALL LOBES: BROWN AND TAN KIDNEY (KD) : -GRANULAR MATERIAL; LEFT, WITHIN PELVIS: MULTIPLE TAN AND FIRM GRANULES, UP TO 1 MM IN DIAMETER HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-MODERATE -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL -FIBROSIS, PERIBILIARY,-MINIMAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-MODERATE -HYPERPLASIA, TRANSITIONAL CELL,MODERATE -INFLAMMATION, SUPPURATIVE, PELVIC,SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,MODERATELY SEVERE ADRENAL, CORTEX (AC) : -PIGMENT,-MINIMAL 3325 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1567 ANIMAL NUMBER: C93010 SEX: FEMALE DATE OF DEATH: 01/10/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 01/10/00 15:04 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 631 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: 377.0 GRAMS PROSECTOR: WENDY CHERRY RECORDER: JODI THIEREN PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 15 X 15 X 10 MM, DARK RED, GRAY AND SOFT UTERUS (UT) : -THICKENED WALL; BILATERAL, DIFFUSE VAGINA (VA) : -MASS(ES); WITHIN LUMEN: SINGLE MASS, 10 X 7 X 7 MM, DARK RED AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (SEE LUMBAR) LN, LUMBAR/ILIAC (LM) : -CYST(S); RIGHT: SINGLE CLEAR FLUID FILLED CYST, 4 X 3 X 3 MM (CAP 21) THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MINIMAL -CYST,-PRESENT THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL MAMMARY, FEMALE (MF) : -HYPERPLASIA, GLANDULAR,-MODERATE UTERUS (UT) : -DILATATION,-MODERATE CERVIX (CV) : -B-POLYP, STROMAL,-NEOPLASM PRESENT VAGINA (VA) : -HYPERPLASIA, MUCINOUS,-SLIGHT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT LN, LUMBAR/ILIAC (LM) : -LYMPHANGIECTASIS,-PRESENT -HYPERPLASIA, LYMPHOPLASMACYTIC,MODERATE 3326 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1568 ANIMAL NUMBER: C93010 SEX: FEMALE DATE OF DEATH: 01/10/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 01/10/00 15:04 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 631 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: 377.0 GRAMS PROSECTOR: WENDY CHERRY RECORDER: JODI THIEREN PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DARK RED AND DRY -ANIMAL THIN -STAINS-PERINEUM/PERIANAL; BROWN AND MOIST MATERIAL 3327 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1569 ANIMAL NUMBER: C93010 SEX: FEMALE DATE OF DEATH: 01/10/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 01/10/00 15:04 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 631 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: 377.0 GRAMS PROSECTOR: WENDY CHERRY RECORDER: JODI THIEREN PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), HEMATO NEOPLASIA (HN) 3328 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1570 ANIMAL NUMBER: C93011 SEX: FEMALE DATE OF DEATH: 04/07/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/07/00 7:24 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 719 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 626.8 GRAMS PROSECTOR: JODI THIEREN RECORDER: JILL PAUS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LUNG (LU) : -LIGHT FOCUS(I)/AREA(S); DIAPHRAGMATIC SURFACE OF RIGHT CAUDAL LOBE: SINGLE TAN AREA, 2 MM IN DIAMETER; COLLECTED INTACT PITUITARY (PI) : -MASS(ES); 8 X 7 X 5 MM, DARK RED, TAN, GRAY AND FIRM UTERUS (UT) : -CYST(S); WITHIN WALL OF LEFT HORN: MULTIPLE CYSTS, SOME ARE CLEAR FLUID FILLED AND SOME ARE RED FLUID FILLED CYSTS, UP TO 5 MM IN DIAMETER HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SLIGHT -MINERALIZATION, PELVIC,-SLIGHT LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PITUITARY (PI) : -HYPERPLASIA,-MODERATE -ANGIECTASIS,-PRESENT UTERUS (UT) : -HYPERPLASIA, CYSTIC, ENDOMETRIAL,MODERATE 3329 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1571 ANIMAL NUMBER: C93011 SEX: FEMALE DATE OF DEATH: 04/07/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/07/00 7:24 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 719 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 626.8 GRAMS PROSECTOR: JODI THIEREN RECORDER: JILL PAUS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY VAGINA (VA) : -MASS(ES); ATTACHED TO SEROSAL SURFACE: SINGLE MASS, 12 X 6 X 2 MM, TAN, SOFT AND VAGINA LIKE APPEARANCE; COLLECTED INTACT; REGIONAL LN (LUMBAR) NORMAL (CAP 22) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN VAGINA (VA) : -HYPERPLASIA, MUCINOUS,-SLIGHT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, LUMBAR/ILIAC (LM) : -HYPERPLASIA, LYMPHOPLASMACYTIC,-SLIGHT 3330 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1572 ANIMAL NUMBER: C93011 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/07/00 STUDY DAY OF DEATH: 719 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 626.8 GRAMS DATE AND TIME OF NECROPSY: 04/07/00 7:24 PROSECTOR: JODI THIEREN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), PARATHYROID (PT), MAMMARY, FEMALE (MF), URINARY BLADDER (UB) 3331 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1573 ANIMAL NUMBER: C93012 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/01/99 STUDY DAY OF DEATH: 561 STUDY WEEK OF DEATH: 81 TERMINAL BODY WEIGHT: 582.3 GRAMS DATE AND TIME OF NECROPSY: 11/01/99 14:40 PROSECTOR: JOHN S. HALFORD RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); AXILLARY REGION-LEFT; WEEK 37; 115 X 95MM; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; BLACK SKIN; VENTRALTHORACIC-LEFT; VERIFIED AT NECROPSY: NOT OBSERVED >SKIN & PELAGE; SORE/SCAB; VENTRALTHORACIC-LEFT; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, PELVIC,-MINIMAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : -PIGMENT,-MODERATELY SEVERE -DEGENERATION / NECROSIS, UNILATERAL,SEVERE ADRENAL, MEDULLA (MA) : -UNILATERALLY EXAMINED,-PRESENT MARROW, FEMUR (FM) : -HYPERPLASIA, ERYTHROID, MARROW,-SLIGHT MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,-MODERATE 3332 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1574 ANIMAL NUMBER: C93012 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/01/99 STUDY DAY OF DEATH: 561 STUDY WEEK OF DEATH: 81 TERMINAL BODY WEIGHT: 582.3 GRAMS DATE AND TIME OF NECROPSY: 11/01/99 14:40 PROSECTOR: JOHN S. HALFORD RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 8 X 6 X 6 MM, RED, GRAY, TAN, AND SOFT STOMACH, GL (ST) : -THICKENED WALL; MUCOSA THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATE PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL STOMACH, GL (ST) : >UNREMARKABLE JEJUNUM (JE) : >AUTOLYTIC ILEUM (IL) : >AUTOLYTIC CECUM (CE) : >AUTOLYTIC MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT -B-FIBROADENOMA,-NEOPLASM PRESENT -HYPERPLASIA, GLANDULAR,-MINIMAL 3333 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1575 ANIMAL NUMBER: C93012 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/01/99 STUDY DAY OF DEATH: 561 STUDY WEEK OF DEATH: 81 TERMINAL BODY WEIGHT: 582.3 GRAMS DATE AND TIME OF NECROPSY: 11/01/99 14:40 PROSECTOR: JOHN S. HALFORD RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS(ES); LEFT AXILLARY REGION, EXTENDING TO LEFT LATERAL THORACIC REGION: SINGLE MASS, 113 X 85 X 61 MM (214.0 GRAMS), RED, DARK RED, GRAY, GREEN, BLACK, TAN, LOBULATED, VASCULAR, CYSTIC, AND FIRM; SOME SECTIONS EXUDE A GREEN MUCOID SEMIFLUID MATERIAL; PORTIONS COLLECTED; REGIONAL LN (SEE AXILLARY); OVERLYING SKIN HAS A SINGLE DRY, BLACK CRUSTED AREA, 65 X 40 MM; COLLECTED WITH MASS -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION LN, AXILLARY (AX) : -LARGE; BILATERAL: LEFT IS UP TO 10 X 8 X 6 MM, RIGHT IS UP TO 7 X 5 X 4 MM (CAP 22) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT LN, AXILLARY (AX) : -HYPERPLASIA, LYMPHOPLASMACYTIC,-SLIGHT 3334 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1576 ANIMAL NUMBER: C93012 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/01/99 STUDY DAY OF DEATH: 561 STUDY WEEK OF DEATH: 81 TERMINAL BODY WEIGHT: 582.3 GRAMS DATE AND TIME OF NECROPSY: 11/01/99 14:40 PROSECTOR: JOHN S. HALFORD RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS) FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL) URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 3335 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1577 ANIMAL NUMBER: C93013 SEX: FEMALE DATE OF DEATH: 12/06/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 12/06/99 15:07 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 596 STUDY WEEK OF DEATH: 86 TERMINAL BODY WEIGHT: 262.1 GRAMS PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; THIN; VERIFIED AT NECROPSY: NOT OBSERVED >EYES; RED DISCHARGE; EYES; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; ATAXIC; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) HISTOPATHOLOGY BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, PELVIC,-MODERATELY SEVERE -INFILTRATE, FAT, INTERSTITIAL,-SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SLIGHT ADRENAL, CORTEX (AC) : -PIGMENT,-MINIMAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),SLIGHT 3336 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1578 ANIMAL NUMBER: C93013 SEX: FEMALE DATE OF DEATH: 12/06/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 12/06/99 15:07 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 596 STUDY WEEK OF DEATH: 86 TERMINAL BODY WEIGHT: 262.1 GRAMS PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 11 X 6 X 6 MM, TAN AND SOFT MAMMARY, FEMALE (MF) : -THICKENED; ALL REGIONS, DIFFUSE: TAN AND SOFT; COLLECTED ON ROUTINE SECTION GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DRY DARK RED MATERIAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 3337 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1579 ANIMAL NUMBER: C93013 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/06/99 STUDY DAY OF DEATH: 596 STUDY WEEK OF DEATH: 86 TERMINAL BODY WEIGHT: 262.1 GRAMS DATE AND TIME OF NECROPSY: 12/06/99 15:07 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 3338 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1580 ANIMAL NUMBER: C93014 SEX: FEMALE DATE OF DEATH: 10/01/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 10/01/99 14:07 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 530 STUDY WEEK OF DEATH: 76 TERMINAL BODY WEIGHT: 253.3 GRAMS PROSECTOR: JILL PAUS RECORDER: TERRI L. WILLIAMS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); INGUINAL REGION-LEFT; WEEK 72; 10 X 10MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); AXILLARY REGION-LEFT; WEEK 73; 10 X 10MM; VERIFIED AT NECROPSY: OBSERVED >EYES; RED DISCHARGE; EYES; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; HEAD TILT; LEFT; VERIFIED AT NECROPSY: NOT APPLICABLE >EXCRETION; NO FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; HUNCHED; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) ADRENAL, CORTEX (AC) : -LARGE; LEFT: 7 X 5 X 3 MM (RIGHT IS 4 X 2 X 2 MM) -DIFFUSELY RED; LEFT: DARK RED HISTOPATHOLOGY BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL -PIGMENT, TUBULAR,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,MODERATELY SEVERE -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC / HEMORRHAGIC,MODERATELY SEVERE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),SLIGHT 3339 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1581 ANIMAL NUMBER: C93014 SEX: FEMALE DATE OF DEATH: 10/01/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 10/01/99 14:07 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 530 STUDY WEEK OF DEATH: 76 TERMINAL BODY WEIGHT: 253.3 GRAMS PROSECTOR: JILL PAUS RECORDER: TERRI L. WILLIAMS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 11 X 7 X 7 MM, RED, DARK RED, TAN AND SOFT STOMACH, NONGL (SU) : -THICKENED WALL; DIFFUSE -RED FOCUS(I)/AREA(S); MUCOSA: MULTIPLE AREAS, PINPOINT AND UP TO 2 X 1 MM MAMMARY, FEMALE (MF) : -CYST(S); ALL REGIONS, DIFFUSE: MULTIPLE TAN CREAMY FLUID FILLED CYSTS, UP TO 12 MM IN DIAMETER (CAP 21); POSSIBLY CONSISTENT WITH MASSES AS NOTED IN CLINICAL OBSERVATIONS UTERUS (UT) : -CYST(S); RIGHT, WITHIN HORN: SINGLE CLEAR FLUID FILLED CYST, 5 MM IN DIAMETER GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DARK RED DRY MATERIAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, NONGL (SU) : -ACANTHOSIS / HYPERKERATOSIS,-MODERATE -ULCERATION,-SLIGHT -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT -INFLAMMATION, CHRONIC,-MODERATE UTERUS (UT) : -CYST,-MODERATE CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 3340 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1582 ANIMAL NUMBER: C93014 SEX: FEMALE DATE OF DEATH: 10/01/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 10/01/99 14:07 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 530 STUDY WEEK OF DEATH: 76 TERMINAL BODY WEIGHT: 253.3 GRAMS PROSECTOR: JILL PAUS RECORDER: TERRI L. WILLIAMS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) (SG), 3341 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1583 ANIMAL NUMBER: C93015 SEX: FEMALE DATE OF DEATH: 03/31/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 03/31/00 11:00 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 712 STUDY WEEK OF DEATH: 102 TERMINAL BODY WEIGHT: 388.7 GRAMS PROSECTOR: JARED FISHEL RECORDER: JARED FISHEL PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); VENTRAL-ABDOMINAL; WEEK 97; 40 X 30MM; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; NO FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >EYES; RED DISCHARGE; EYES; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) ADRENAL, CORTEX (AC) : -LARGE; BILATERAL: EACH IS 7 MM IN DIAMETER HISTOPATHOLOGY BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, PERIPORTAL,-SLIGHT KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-SLIGHT ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATELY SEVERE -HYPERTROPHY, CORTICAL CELL,-SLIGHT -DEGENERATION, CYSTIC / HEMORRHAGIC,SLIGHT -PIGMENT,-SLIGHT 3342 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1584 ANIMAL NUMBER: C93015 SEX: FEMALE DATE OF DEATH: 03/31/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 03/31/00 11:00 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 712 STUDY WEEK OF DEATH: 102 TERMINAL BODY WEIGHT: 388.7 GRAMS PROSECTOR: JARED FISHEL RECORDER: JARED FISHEL PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 8 MM IN DIAMETER, DARK RED AND SOFT MAMMARY, FEMALE (MF) : -MASS(ES); MID ABDOMINAL REGION: SINGLE MASS, 45 X 30 X 30 MM, RED, TAN AND LOBULATED; SOME SECTIONS EXUDE A TAN SEMIFLUID; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION UTERUS (UT) : -MASS(ES); WITHIN LUMEN OF LEFT HORN: SINGLE MASS, 25 X 4 X 3 MM, RED, TAN AND SOFT; COLLECTED INTACT WITH UTERUS; REGIONAL LN (LUMBAR) NORMAL (CAP 21) THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),SLIGHT LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL HEART (HT) : -CARDIOMYOPATHY,-MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT -B-FIBROADENOMA,-NEOPLASM PRESENT UTERUS (UT) : -B-POLYP, ENDOMETRIAL, STROMAL,NEOPLASM PRESENT -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT 3343 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1585 ANIMAL NUMBER: C93015 SEX: FEMALE DATE OF DEATH: 03/31/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 03/31/00 11:00 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 712 STUDY WEEK OF DEATH: 102 TERMINAL BODY WEIGHT: 388.7 GRAMS PROSECTOR: JARED FISHEL RECORDER: JARED FISHEL PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DRY RED MATERIAL -STAINS-PERINEUM/PERIANAL; PERINEUM: MOIST YELLOW MATERIAL CERVIX (CV) : -HYPERPLASIA, STROMAL,-MODERATE CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT LN, LUMBAR/ILIAC (LM) : -HYPERPLASIA, LYMPHOPLASMACYTIC,MODERATE 3344 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1586 ANIMAL NUMBER: C93015 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/31/00 STUDY DAY OF DEATH: 712 STUDY WEEK OF DEATH: 102 TERMINAL BODY WEIGHT: 388.7 GRAMS DATE AND TIME OF NECROPSY: 03/31/00 11:00 PROSECTOR: JARED FISHEL RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), VAGINA (VA), HEMATO NEOPLASIA (HN) 3345 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1587 ANIMAL NUMBER: C93016 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/11/99 STUDY DAY OF DEATH: 571 STUDY WEEK OF DEATH: 82 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/11/99 7:46 PROSECTOR: CHANESTA ACKER RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; MISSING; TEETH; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; ROUGH HAIR COAT; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; HEAD TILT; RIGHT; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; THIN; VERIFIED AT NECROPSY: NOT OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) KIDNEY (KD) : -LARGE PELVIS(ES); RIGHT PANCREAS (PA) : -MASS(ES); ATTACHED TO CRANIAL PORTION OF DUODENUM: SINGLE MASS, 17 X 14 X 10 MM, RED, DARK RED, TAN AND SOFT; ON SECTION EXUDES A RED, SEMIFLUID MATERIAL (CAP 22); REGIONAL LN (SEE PANCREATIC) ADRENAL, CORTEX (AC) : -LARGE; LEFT: 7 X 5 X 3 MM (RIGHT IS 5 X 4 X 3 MM) HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -HEMORRHAGE,-MINIMAL LIVER (LI) : -HYPERTROPHY, HEPATOCELLULAR, PERIPORTAL,-MINIMAL -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL KIDNEY (KD) : -DILATATION, PELVIC,-MODERATE SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE PANCREAS (PA) : -M-CARCINOMA, ISLET CELL,-NEOPLASM PRESENT ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT -PIGMENT,-MINIMAL MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,-SLIGHT 3346 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1588 ANIMAL NUMBER: C93016 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/11/99 STUDY DAY OF DEATH: 571 STUDY WEEK OF DEATH: 82 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/11/99 7:46 PROSECTOR: CHANESTA ACKER RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY LUNG (LU) : -UNCOLLAPSED; ALL LOBES PITUITARY (PI) : -MASS(ES); 12 X 10 X 8 MM, TAN, GREY AND SOFT DARK RED, LN, MANDIBULAR (MN) : -LARGE; BILATERAL: EACH IS UP TO 12 X 6 X 3 MM (CAP 21) LN, ANT MES/PANC (AP) : -DIFFUSELY RED; DARK RED (CAP 23) MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,-MODERATE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),SLIGHT LUNG (LU) : >UNREMARKABLE PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT JEJUNUM (JE) : >AUTOLYTIC ILEUM (IL) : >AUTOLYTIC CECUM (CE) : >AUTOLYTIC MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT -HYPERPLASIA, GLANDULAR,-SLIGHT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT LN, MANDIBULAR (MN) : -HYPERPLASIA, LYMPHOPLASMACYTIC,MODERATELY SEVERE LN, ANT MES/PANC (AP) : -HEMORRHAGE,-PRESENT -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL 3347 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1589 ANIMAL NUMBER: C93016 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/11/99 STUDY DAY OF DEATH: 571 STUDY WEEK OF DEATH: 82 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/11/99 7:46 PROSECTOR: CHANESTA ACKER RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -MALOCCLUSION; UPPER RIGHT INCISOR -TEETH MISSING/BROKEN/WORN; UPPER LEFT INCISOR: MISSING THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), SPLEEN (SP), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), TAIL (TI), LN, LUMBAr /i LIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 3348 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1590 ANIMAL NUMBER: C93017 SEX: FEMALE DATE OF DEATH: 01/10/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 01/10/00 14:58 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 631 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: 464.5 GRAMS PROSECTOR: JAMES T. CALLEN RECORDER: JODI THIEREN PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK 33; 60 X 60MM; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; VENTRALABDOMINAL-LEFT; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) OVARY (OV) : -CYST(S); RIGHT: SINGLE RED FLUID FILLED CYST, 1 MM IN DIAMETER HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -NECROSIS, INDIVIDUAL HEPATOCYTE,MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATELY SEVERE ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL -PIGMENT,-SLIGHT OVARY (OV) : >UNREMARKABLE MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,-SEVERE MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,-SEVERE 3349 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1591 ANIMAL NUMBER: C93017 SEX: FEMALE DATE OF DEATH: 01/10/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 01/10/00 14:58 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 631 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: 464.5 GRAMS PROSECTOR: JAMES T. CALLEN RECORDER: JODI THIEREN PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY THYROID (TY) : -LARGE; LEFT: 6 X 4 X 3 MM (RIGHT IS 4 X 3 X 2 MM) PITUITARY (PI) : -MASS(ES); 10 X 7 X 6 MM, DARK RED AND FIRM SKIN (SK) : >NOTE:>SKIN AND MAMMARY SECTION TAKEN FROM RIGHT INGUINAL REGION DUE TO LEFT INGUINAL MASS MAMMARY, FEMALE (MF) : -MASS(ES); LEFT INGUINAL REGION: SINGLE MASS, 72 X 65 X 39 MM, TAN, RED, LOBULATED AND FIRM; SAME ON CUT SURFACE; OVERLYING SKIN HAS A RED, TAN AND MOIST ULCERATED AREA, 37 X 20 MM; COLLECTED INTACT WITH MASS; REGIONAL LN (INGUINAL) NOT IDENTIFIED UTERUS (UT) : -CYST(S); BILATERAL, WITHIN WALLS: MULTIPLE CLEAR FLUID FILLED CYSTS, UP TO 4 MM IN DIAMETER THYMUS (TH) : >AUTOLYTIC HEART (HT) : -CARDIOMYOPATHY,-MINIMAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -M-CARCINOMA,-NEOPLASM PRESENT -B-FIBROADENOMA,-NEOPLASM PRESENT -HYPERPLASIA, GLANDULAR,-MINIMAL >NOTE:>MASS 1 IS THE CARCINOMA UTERUS (UT) : -DILATATION,-MODERATELY SEVERE VAGINA (VA) : -HYPERPLASIA, MUCINOUS,-SLIGHT 3350 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1592 ANIMAL NUMBER: C93017 SEX: FEMALE DATE OF DEATH: 01/10/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 01/10/00 14:58 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 631 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: 464.5 GRAMS PROSECTOR: JAMES T. CALLEN RECORDER: JODI THIEREN PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) -BLOOD FILM TAKEN CAUSE OF DEATH (CD) : -NEOPLASM, MAMMARY GLAND,-PRESENT >NOTE:>CARCINOMA THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUm /s KULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), HEMATO NEOPLASIA (HN) 3351 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1593 ANIMAL NUMBER: C93018 SEX: FEMALE DATE OF DEATH: 07/21/98 STUDY DAY OF DATE AND TIME OF NECROPSY: 07/21/98 13:57 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 275.9 GRAMS PROSECTOR: LAURIE J. SCHULLER RECORDER: LYNN HORNER PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) LF ADRENAL (AD0) RT ADRENAL (AD1) BRAIN (BR) LF KIDNEY (KD0) RT KIDNEY (KD1) LIVER (LI) LUNG (LU) LF OVARY (OV0) RT OVARY (OV1) SPLEEN (SP) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) UTERUS (UT) .0335 .0363 2.0491 1.1939 1.1675 9.0596 1.4142 .0709 .0673 .4924 .0056 .0105 .5391 .0121 % .0132 % .7427 % .4327 % .4232 % 3.2837 % .5126 % .0257 % .0244 % .1785 % .0020 % .0038 % .1954 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0163 .0177 1.0000 .5826 .5698 4.4213 .6902 .0346 .0328 .2403 .0027 .0051 .2631 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-MINIMAL KIDNEY (KD) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -MINERALIZATION, TUBULAR,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MINIMAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 3352 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1594 ANIMAL NUMBER: C93018 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 275.9 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 13:57 PROSECTOR: LAURIE J. SCHULLER RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV) 3353 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1595 ANIMAL NUMBER: C93019 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/22/99 STUDY DAY OF DEATH: 309 STUDY WEEK OF DEATH: 45 TERMINAL BODY WEIGHT: 292.6 GRAMS DATE AND TIME OF NECROPSY: 02/22/99 13:47 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; RED HAIR COAT; HEADENTIRE; VERIFIED AT NECROPSY: NOT OBSERVED >SKIN & PELAGE; YELLOW HAIR COAT; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; HUNCHED; VERIFIED AT NECROPSY: NOT APPLICABLE >EXCRETION; NO FECES; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-SLIGHT -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-MODERATE KIDNEY (KD) : -MINERALIZATION, TUBULAR,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-SLIGHT ADRENAL, CORTEX (AC) : -INFILTRATE, MACROPHAGE, PIGMENTED,SLIGHT MARROW, FEMUR (FM) : -HYPOCELLULAR, MARROW,-MODERATE MARROW, STERNUM (SE) : -HYPOCELLULAR, MARROW,-MODERATE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),SLIGHT 3354 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1596 ANIMAL NUMBER: C93019 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/22/99 STUDY DAY OF DEATH: 309 STUDY WEEK OF DEATH: 45 TERMINAL BODY WEIGHT: 292.6 GRAMS DATE AND TIME OF NECROPSY: 02/22/99 13:47 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 10 X 9 X 9 MM, RED AND SOFT STOMACH, NONGL (SU) : -THICKENED WALL; DIFFUSE RED, DARK GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -STAINS-PERINEUM/PERIANAL; MOIST YELLOW MATERIAL -DISCHARGE-NASAL; BILATERAL: DARK RED AND DRY MATERIAL LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, NONGL (SU) : -ACANTHOSIS / HYPERKERATOSIS,-SLIGHT -EDEMA,-PRESENT -INFLAMMATION, SUBACUTE,-MODERATE MAMMARY, FEMALE (MF) : -HYPERPLASIA, GLANDULAR,-SLIGHT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 3355 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1597 ANIMAL NUMBER: C93019 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/22/99 STUDY DAY OF DEATH: 309 STUDY WEEK OF DEATH: 45 TERMINAL BODY WEIGHT: 292.6 GRAMS DATE AND TIME OF NECROPSY: 02/22/99 13:47 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 3356 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1598 ANIMAL NUMBER: C93020 SEX: FEMALE DATE OF DEATH: 07/24/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 07/24/99 9:32 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DEATH: 461 STUDY WEEK OF DEATH: 66 TERMINAL BODY WEIGHT: NOT ENTERED PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: NOT OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) THYMUS (TH) : -RED FOCUS(I)/AREA(S); DIFFUSE: MULTIPLE RED FOCI, UP TO 2 MM IN DIAMETER HISTOPATHOLOGY BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -NECROSIS, INDIVIDUAL HEPATOCYTE,MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-SLIGHT -CONGESTION,-PRESENT KIDNEY (KD) : -MINERALIZATION, PELVIC,-SLIGHT -INFLAMMATION, SUPPURATIVE, PELVIC,MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE PANCREAS (PA) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL ADRENAL, CORTEX (AC) : -PIGMENT,-SLIGHT THYMUS (TH) : -HEMORRHAGE,-PRESENT 3357 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1599 ANIMAL NUMBER: C93020 SEX: FEMALE DATE OF DEATH: 07/24/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 07/24/99 9:32 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DEATH: 461 STUDY WEEK OF DEATH: 66 TERMINAL BODY WEIGHT: NOT ENTERED PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 8 X 6 X 5 MM, RED, GRAY, TAN, LOBULATED AND SOFT UTERUS (UT) : -LUMEN FILLED WITH FLUID; BILATERAL, NEAR DISTAL ENDS: DARK GREEN, MUCOID SEMIFLUID LN, MEDIASTINAL (ML) : -DIFFUSELY RED; DARK RED (CAP 20) GENERAL COMMENT (GC) : -DISCHARGE-OCULAR; BILATERAL: DRY, MATERIAL RED PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL JEJUNUM (JE) : >AUTOLYTIC ILEUM (IL) : >AUTOLYTIC CECUM (CE) : >AUTOLYTIC COLON (CO) : >AUTOLYTIC MAMMARY, FEMALE (MF) : >SECTION EXAMINED; TISSUE NOT PRESENT UTERUS (UT) : >UNREMARKABLE CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT LN, MEDIASTINAL (ML) : -HYPERPLASIA, LYMPHOPLASMACYTIC,-SLIGHT 3358 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1600 ANIMAL NUMBER: C93020 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 07/24/99 STUDY DAY OF DEATH: 461 STUDY WEEK OF DEATH: 66 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 07/24/99 9:32 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 3359 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1601 ANIMAL NUMBER: C93021 SEX: FEMALE DATE OF DEATH: 06/28/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 06/28/99 13:45 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 435 STUDY WEEK OF DEATH: 63 TERMINAL BODY WEIGHT: 291.5 GRAMS PROSECTOR: KEVIN BILLINGS RECORDER: KEVIN BILLINGS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; HEAD TILT; RIGHT; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; THIN; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; ROUGH HAIR COAT; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; YELLOW HAIR COAT; VERIFIED AT NECROPSY: NOT OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) HISTOPATHOLOGY BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -HYPERPLASIA, BILE DUCT,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, PERIPORTAL,-MINIMAL -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, PELVIC,-SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : -PIGMENT,-MINIMAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),SLIGHT LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL 3360 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1602 ANIMAL NUMBER: C93021 SEX: FEMALE DATE OF DEATH: 06/28/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 06/28/99 13:45 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 435 STUDY WEEK OF DEATH: 63 TERMINAL BODY WEIGHT: 291.5 GRAMS PROSECTOR: KEVIN BILLINGS RECORDER: KEVIN BILLINGS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); SINGLE MASS, 12 X 8 X 6 MM; DARK RED, TAN, GREY, SOFT AND LOBULATED MAMMARY, FEMALE (MF) : -THICKENED; ALL REGIONS DIFFUSE: TAN; COLLECTED ON ROUTINE SECTION -CYST(S); LEFT PERIANAL REGION: SINGLE CYST, 10 MM IN DIAMETER; FILLED WITH A TAN CREAMY SEMIFLUID (CAP 20) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT -HYPERPLASIA, GLANDULAR,-MODERATE CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 3361 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1603 ANIMAL NUMBER: C93021 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/28/99 STUDY DAY OF DEATH: 435 STUDY WEEK OF DEATH: 63 TERMINAL BODY WEIGHT: 291.5 GRAMS DATE AND TIME OF NECROPSY: 06/28/99 13:45 PROSECTOR: KEVIN BILLINGS RECORDER: KEVIN BILLINGS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 3362 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1604 ANIMAL NUMBER: C93022 SEX: FEMALE DATE OF DEATH: 04/07/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/07/00 7:24 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 719 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 591.1 GRAMS PROSECTOR: TROY ELLENBECKER RECORDER: JILL PAUS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); VENTRAL-ABDOMINAL-RIGHT; WEEK 87; 60 X 50MM; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE DARK RED FOCI, SOME SUNKEN WITH A STELLATE PATTERN, UP TO 5 MM IN DIAMETER; COLLECTED ON ROUTINE SECTIONS ADRENAL, CORTEX (AC) : -LARGE; LEFT: 7 MM IN DIAMETER (RIGHT IS 4 MM IN DIAMETER) -DARK FOCUS(I)/AREA(S); LEFT: SINGLE DARK BROWN AREA, 3 MM IN DIAMETER PITUITARY (PI) : -RED FOCUS(I)/AREA(S); RIGHT ASPECT: SINGLE DARK RED AREA, 2 X 1 MM HISTOPATHOLOGY LIVER (LI) : -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-SLIGHT -ANGIECTASIS,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SLIGHT -MINERALIZATION, PELVIC,-MODERATE ADRENAL, CORTEX (AC) : -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL -HYPERTROPHY, CORTICAL CELL,-MODERATE -DEGENERATION, CYSTIC / HEMORRHAGIC,MODERATE -PIGMENT,-MINIMAL ADRENAL, MEDULLA (MA) : -UNILATERALLY EXAMINED,-PRESENT THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PITUITARY (PI) : >NOT REQUIRED TO BE EXAMINED FOR ANIMAL 3363 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1605 ANIMAL NUMBER: C93022 SEX: FEMALE DATE OF DEATH: 04/07/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/07/00 7:24 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 719 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 591.1 GRAMS PROSECTOR: TROY ELLENBECKER RECORDER: JILL PAUS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT INGUINAL REGION: SINGLE MASS, 60 X 50 X 30 MM, RED, DARK RED, TAN AND FIRM; SAME ON CUT SURFACE (CAP 22); REGIONAL LN (INGUINAL) NOT IDENTIFIED -MASS #2; LEFT INGUINAL REGION: SINGLE MASS, 30 X 30 X 15 MM, RED, TAN AND FIRM; SAME ON CUT SURFACE (CAP 23); REGIONAL LN (INGUINAL) NOT IDENTIFIED UTERUS (UT) : -CYST(S); RIGHT HORN, NEAR BIFURCATION: SINGLE BROWN FLUID FILLED CYST, 5 MM IN DIAMETER; CYST RUPTURED AT NECROPSY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT -B-FIBROADENOMA,- 2 NEOPLASMS PRESENT -HYPERPLASIA, GLANDULAR,-SLIGHT UTERUS (UT) : >UNREMARKABLE CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 3364 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1606 ANIMAL NUMBER: C93022 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/07/00 STUDY DAY OF DEATH: 719 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 591.1 GRAMS DATE AND TIME OF NECROPSY: 04/07/00 7:24 PROSECTOR: TROY ELLENBECKER RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), LUNG (LU), PARATHYROID (PT), URINARY BLADDER (UB) 3365 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1607 ANIMAL NUMBER: C93023 SEX: FEMALE DATE OF DEATH: 07/21/98 STUDY DAY OF DATE AND TIME OF NECROPSY: 07/21/98 10:30 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 237.2 GRAMS PROSECTOR: STEVE VAN ADESTINE RECORDER: KIMBERLY DURLAND PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) LF ADRENAL (AD0) RT ADRENAL (AD1) BRAIN (BR) LF KIDNEY (KD0) RT KIDNEY (KD1) LIVER (LI) LUNG (LU) LF OVARY (OV0) RT OVARY (OV1) SPLEEN (SP) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) UTERUS (UT) .0484 .0402 2.0570 1.1140 1.0549 7.8631 1.1581 .0665 .0589 .4895 .0077 .0074 .5715 .0204 % .0169 % .8672 % .4696 % .4447 % 3.3150 % .4882 % .0280 % .0248 % .2064 % .0032 % .0031 % .2409 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0235 .0195 1.0000 .5416 .5128 3.8226 .5630 .0323 .0286 .2380 .0037 .0036 .2778 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-SLIGHT KIDNEY (KD) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -DILATATION, PELVIC,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 3366 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1608 ANIMAL NUMBER: C93023 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 237.2 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 10:30 PROSECTOR: STEVE VAN ADESTINE RECORDER: KIMBERLY DURLAND POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV) 3367 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1609 ANIMAL NUMBER: C93024 SEX: FEMALE DATE OF DEATH: 12/08/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 12/08/99 11:39 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 598 STUDY WEEK OF DEATH: 86 TERMINAL BODY WEIGHT: 313.7 GRAMS PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK 61; 60 X 70MM; VERIFIED AT NECROPSY: OBSERVED >EYES; RED DISCHARGE; EYES; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >RESPIRATION; IRREGULAR; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; HUNCHED; VERIFIED AT NECROPSY: NOT APPLICABLE >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) HISTOPATHOLOGY BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, PELVIC,-MINIMAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : -HYPERTROPHY, CORTICAL CELL,-MINIMAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),SLIGHT LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL HEART (HT) : -CARDIOMYOPATHY,-MINIMAL 3368 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1610 ANIMAL NUMBER: C93024 SEX: FEMALE DATE OF DEATH: 12/08/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 12/08/99 11:39 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 598 STUDY WEEK OF DEATH: 86 TERMINAL BODY WEIGHT: 313.7 GRAMS PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 10 X 10 X 4 MM, DARK RED AND SOFT MAMMARY, FEMALE (MF) : -MASS(ES); LEFT VENTRAL ABDOMINAL REGION: SINGLE MASS, 65 X 40 X 30 MM, TAN, SOFT, VASCULAR, AND LOBULATED; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (INGUINAL) NOT IDENTIFIED GENERAL COMMENT (GC) -BLOOD FILM TAKEN -DISCHARGE-OCULAR; RED MATERIAL -ANIMAL THIN : BILATERAL: DRY DARK THYROID (TY) : -HYPERPLASIA, C-CELL,-SLIGHT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT -B-FIBROADENOMA,-NEOPLASM PRESENT -HYPERPLASIA, GLANDULAR,-SLIGHT VAGINA (VA) : -HYPERPLASIA, MUCINOUS,-MINIMAL CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 3369 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1611 ANIMAL NUMBER: C93024 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/08/99 STUDY DAY OF DEATH: 598 STUDY WEEK OF DEATH: 86 TERMINAL BODY WEIGHT: 313.7 GRAMS DATE AND TIME OF NECROPSY: 12/08/99 11:39 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), HEMATO NEOPLASIA (HN) (ST), (SK), 3370 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1612 ANIMAL NUMBER: C93025 SEX: FEMALE DATE OF DEATH: 03/23/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 03/23/00 15:03 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DEATH: 704 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: NOT ENTERED PROSECTOR: JERALD W. JOHNSON RECORDER: JERALD W. JOHNSON PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: NOT OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) HISTOPATHOLOGY BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MODERATE SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE PANCREAS (PA) : -INFILTRATION, FAT, INTERSTITIAL,SLIGHT ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-MINIMAL -ANGIECTASIS,-MINIMAL -PIGMENT,-MINIMAL ADRENAL, MEDULLA (MA) : -B-PHEOCHROMOCYTOMA,-NEOPLASM PRESENT MARROW, FEMUR (FM) : -HYPOCELLULAR, MARROW,-MODERATE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),SLIGHT -CYST,-PRESENT 3371 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1613 ANIMAL NUMBER: C93025 SEX: FEMALE DATE OF DEATH: 03/23/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 03/23/00 15:03 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DEATH: 704 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: NOT ENTERED PROSECTOR: JERALD W. JOHNSON RECORDER: JERALD W. JOHNSON PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) -MASS(ES); 7 X 7 X 5 MM, DARK RED, TAN AND SOFT MAMMARY, FEMALE (MF) : -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM CERVIX (CV) : -LARGE; 8 MM IN DIAMETER GENERAL COMMENT (GC) : -DISCHARGE-OCULAR; BILATERAL: RED, MOIST MATERIAL -DISCHARGE-NASAL; RED, DRY MATERIAL LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT DUODENUM (DU) : >AUTOLYTIC JEJUNUM (JE) : >AUTOLYTIC MAMMARY, FEMALE (MF) : -HYPERPLASIA, GLANDULAR,-MODERATE URINARY BLADDER (UB) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL CERVIX (CV) : -HYPERPLASIA, MUCINOUS,-MODERATE VAGINA (VA) : -LUMEN, EXUDATE,-PRESENT -HYPERPLASIA, MUCINOUS,-MODERATELY SEVERE CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 3372 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1614 ANIMAL NUMBER: C93025 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/23/00 STUDY DAY OF DEATH: 704 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/23/00 15:03 PROSECTOR: JERALD W. JOHNSON RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUm /s KULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), KIDNEY (KD), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), UTERUS (UT), HEMATO NEOPLASIA (HN) 3373 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1615 ANIMAL NUMBER: C93026 SEX: FEMALE DATE OF DEATH: 04/03/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/03/00 13:29 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 715 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 331.0 GRAMS PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); AXILLARY REGION-LEFT; WEEK 92; 40 X 30MM; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; BLUE SKIN; VENTRALTHORACIC-LEFT; VERIFIED AT NECROPSY: NOT APPLICABLE >EYES; PALE; EYES; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; VENTRALTHORACIC-LEFT; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -DIFFUSELY LIGHT; ALL LOBES: TAN KIDNEY (KD) : -GRANULAR MATERIAL; RIGHT, WITHIN PELVIS: MULTIPLE TAN PINPOINT GRANULES -DIFFUSELY LIGHT; BILATERAL: TAN SPLEEN (SP) : -LARGE; 48 X 12 X 6 MM HISTOPATHOLOGY BRAIN (BR) : >UNREMARKABLE LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -NECROSIS, COAGULATIVE,-MODERATE -HEMATOPOIESIS, EXTRAMEDULLARY,MODERATE -HYPERPLASIA, BILE DUCT,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, PERIPORTAL,-MODERATE KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MODERATE -MINERALIZATION, PELVIC,-MINIMAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SEVERE PANCREAS (PA) : -HYPERTROPHY / BASOPHILIA, ACINAR CELL,SLIGHT -INFLAMMATION, VASCULAR,-MODERATELY SEVERE 3374 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1616 ANIMAL NUMBER: C93026 SEX: FEMALE DATE OF DEATH: 04/03/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/03/00 13:29 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 715 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 331.0 GRAMS PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY ADRENAL, CORTEX (AC) : -LARGE; LEFT: 6 MM IN DIAMETER (RIGHT IS 4 MM IN DIAMETER) OVARY (OV) : -CYST(S); LEFT: SINGLE CLEAR FLUID FILLED CYST, 4 MM IN DIAMETER PITUITARY (PI) : -MASS(ES); 4 X 4 X 3 MM, RED, GREY, CYSTIC AND SOFT ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATELY SEVERE -HEMATOPOIESIS, EXTRAMEDULLARY,-SLIGHT -HYPERTROPHY, CORTICAL CELL,-MINIMAL -PIGMENT,-MINIMAL OVARY (OV) : -CYST,-PRESENT MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,-SEVERE MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,MODERATELY SEVERE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY), SLIGHT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,MODERATE -INFLAMMATION, VASCULAR,-MODERATE MAMMARY, FEMALE (MF) : -M-CARCINOMA,-NEOPLASM PRESENT -CYST / GALACTOCELE,-PRESENT -HYPERPLASIA, GLANDULAR,-SLIGHT 3375 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1617 ANIMAL NUMBER: C93026 SEX: FEMALE DATE OF DEATH: 04/03/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/03/00 13:29 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 715 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 331.0 GRAMS PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS(ES); LEFT AXILLARY REGION: SINGLE MASS, 21 X 19 X 11 MM, RED, DARK RED, GREY, TAN AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (SEE AXILLARY); OVERLYING SKIN HAS A SINGLE DRY, RED AND TAN CRUSTED AREA, 15 X 11 MM; COLLECTED WITH MASS; POSSIBLY CONSISTENT WITH SCAB (VENTRAL-THORACIC-LEFT) AS NOTED IN CLINICAL OBSERVATIONS LN, AXILLARY (AX) : -LARGE; LEFT: UP TO 5 X 4 X 3 MM (RIGHT IS UP TO 4 X 3 X 2 MM) (CAP 21) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL LOWER AND UPPER INCISORS -DISCHARGE-OCULAR; LEFT: DRY, RED MATERIAL -ANIMAL THIN CERVIX (CV) : -HYPERPLASIA, MUCINOUS,-MINIMAL VAGINA (VA) : -LUMEN, EXUDATE,-PRESENT -HYPERPLASIA, MUCINOUS,-MODERATE CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT LN, AXILLARY (AX) : -HYPERPLASIA, LYMPHOPLASMACYTIC,MODERATELY SEVERE 3376 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1618 ANIMAL NUMBER: C93026 SEX: FEMALE DATE OF DEATH: 04/03/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/03/00 13:29 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 715 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 331.0 GRAMS PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG),STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP),JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM(DP), CAVITY, THORACIC (TA),LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), HEMATO NEOPLASIA (HN) 3377 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1619 ANIMAL NUMBER: C93027 SEX: FEMALE DATE OF DEATH: 01/10/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 01/10/00 14:48 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 631 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: 344.8 GRAMS PROSECTOR: WENDY CHERRY RECORDER: JODI THIEREN PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >EYES; RED DISCHARGE; EYES; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; ROUGH HAIR COAT; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) KIDNEY (KD) : -GRANULAR MATERIAL; LEFT, WITHIN PELVIS: MULTIPLE TAN AND FIRM GRANULES, UP TO 1 MM IN DIAMETER HISTOPATHOLOGY BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-SLIGHT -HYPERPLASIA, BILE DUCT,-MINIMAL -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, PELVIC,-SLIGHT -HYPERPLASIA, TRANSITIONAL CELL,-SLIGHT -INFLAMMATION, SUPPURATIVE, PELVIC,MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT -PIGMENT,-SLIGHT 3378 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1620 ANIMAL NUMBER: C93027 SEX: FEMALE DATE OF DEATH: 01/10/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 01/10/00 14:48 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 631 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: 344.8 GRAMS PROSECTOR: WENDY CHERRY RECORDER: JODI THIEREN PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 20 X 15 X 10 MM, DARK RED, TAN AND SOFT MAMMARY, FEMALE (MF) : -CYST(S); ALL REGIONS, DIFFUSE: MULTIPLE TAN AND CREAMY SEMIFLUID FILLED CYSTS, UP TO 5 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION VAGINA (VA) : -SEMIFLUID MATERIAL; WITHIN LUMEN: GREEN GENERAL COMMENT (GC) -BLOOD FILM TAKEN -DISCHARGE-OCULAR; AND DRY -ANIMAL THIN : BILATERAL: DARK RED THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATE PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT -HYPERPLASIA, GLANDULAR,-MODERATE UTERUS (UT) : -HYPERPLASIA, MINIMAL VAGINA (VA) : -HYPERPLASIA, CYSTIC, ENDOMETRIAL,MUCINOUS,-SLIGHT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 3379 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1621 ANIMAL NUMBER: C93027 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/10/00 STUDY DAY OF DEATH: 631 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: 344.8 GRAMS DATE AND TIME OF NECROPSY: 01/10/00 14:48 PROSECTOR: WENDY CHERRY RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), HEMATO NEOPLASIA (HN) 3380 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1622 ANIMAL NUMBER: C93028 SEX: FEMALE DATE OF DEATH: 01/17/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 01/17/00 14:52 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 638 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: 338.8 GRAMS PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); INGUINAL REGION-RIGHT; WEEK 88; 10 X10MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); VENTRAL-ABDOMINAL-RIGHT; WEEK 91; 15 X 15MM; VERIFIED AT NECROPSY: OBSERVED >EYES; RED DISCHARGE; EYES; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) HISTOPATHOLOGY BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MODERATE -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-MODERATE SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATELY SEVERE -PIGMENT,-SLIGHT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATE HEART (HT) : -CARDIOMYOPATHY,-MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-SLIGHT 3381 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1623 ANIMAL NUMBER: C93028 SEX: FEMALE DATE OF DEATH: 01/17/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 01/17/00 14:52 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 638 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: 338.8 GRAMS PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 12 X 9 X 7 MM, DARK RED AND FIRM MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT INGUINAL REGION: SINGLE MASS, 20 MM IN DIAMETER, TAN, RED, AND FIRM; SAME ON CUT SURFACE; (CAP 21); REGIONAL LN (INGUINAL) NOT IDENTIFIED -MASS #2; RIGHT INGUINAL REGION CRANIAL TO MASS 1: SINGLE MASS, 15 X 12 X 10 MM, TAN, RED AND FIRM; SAME ON CUT SURFACE; (CAP 22); REGIONAL LN (INGUINAL) NOT IDENTIFIED GENERAL COMMENT (GC) -BLOOD FILM TAKEN -DISCHARGE-OCULAR; MATERIAL -ANIMAL THIN : BILATERAL: DRY RED PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -M-CARCINOMA,-NEOPLASM PRESENT -CYST / GALACTOCELE,-PRESENT -B-FIBROADENOMA,-NEOPLASM PRESENT -HYPERPLASIA, GLANDULAR,-MODERATE >NOTE:>MASS #1 IS THE FIBROADENOMA VAGINA (VA) : -HYPERPLASIA, MUCINOUS,-MODERATE CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 3382 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1624 ANIMAL NUMBER: C93028 SEX: FEMALE DATE OF DEATH: 01/17/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 01/17/00 14:52 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 638 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: 338.8 GRAMS PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), HEMATO NEOPLASIA (HN) (SM), (SG), 3383 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1625 ANIMAL NUMBER: C93029 SEX: FEMALE DATE OF DEATH: 09/01/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 09/01/99 14:25 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 500 STUDY WEEK OF DEATH: 72 TERMINAL BODY WEIGHT: 282.0 GRAMS PROSECTOR: JERALD W. JOHNSON RECORDER: JERALD W. JOHNSON PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); VENTRAL-CERVICAL; WEEK 33; 40 X 20MM; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: NOT OBSERVED >SKIN & PELAGE; ROUGH HAIR COAT; VERIFIED AT NECROPSY: NOT APPLICABLE >EXCRETION; NO FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; SEVERE; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; HEAD TILT; LEFT; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) ADRENAL, CORTEX (AC) : -DIFFUSELY RED; BILATERAL: DARK RED OVARY (OV) : -CYST(S); LEFT: SINGLE CLEAR FLUID FILLED CYST, 5 MM IN DIAMETER PITUITARY (PI) : -LARGE; 7 X 6 X 5 MM; APPEARS SWOLLEN -DARK FOCUS(I)/AREA(S); RIGHT AND LEFT ANTERIOR ASPECT: SINGLE DARK BROWN FOCUS AT EACH LOCATION, LEFT IS 1 MM IN DIAMETER, RIGHT IS 3 MM IN DIAMETER HISTOPATHOLOGY BRAIN (BR) : >UNREMARKABLE LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT -FIBROSIS, CAPSULAR,-PRESENT ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT OVARY (OV) : -CYST,-PRESENT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATE HEART (HT) : -CARDIOMYOPATHY,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT 3384 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1626 ANIMAL NUMBER: C93029 SEX: FEMALE DATE OF DEATH: 09/01/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 09/01/99 14:25 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 500 STUDY WEEK OF DEATH: 72 TERMINAL BODY WEIGHT: 282.0 GRAMS PROSECTOR: JERALD W. JOHNSON RECORDER: JERALD W. JOHNSON PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: MULTIPLE DARK BROWN AREAS, UP TO 2 X 1 MM MAMMARY, FEMALE (MF) : -MASS(ES); VENTRAL CERVICAL REGION: SINGLE MASS, 35 X 30 X 15 MM, TAN, DARK BROWN, RED, LOBULATED AND FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (MANDIBULAR) NORMAL (CAP 20) -THICKENED; ALL REGIONS: DIFFUSE; COLLECTED ON ROUTINE SECTION UTERUS (UT) : -DIFFUSELY RED; BILATERAL GENERAL COMMENT (GC) : -BLOOD FILM TAKEN STOMACH, GL (ST) : >UNREMARKABLE RECTUM (RE) : -PARASITISM,-PRESENT MAMMARY, FEMALE (MF) : -M-CARCINOMA,-NEOPLASM PRESENT -CYST / GALACTOCELE,-PRESENT UTERUS (UT) : >UNREMARKABLE VAGINA (VA) : -LUMEN, EXUDATE,-PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT LN, MANDIBULAR (MN) : -HYPERPLASIA, LYMPHOPLASMACYTIC,MODERATE 3385 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1627 ANIMAL NUMBER: C93029 SEX: FEMALE DATE OF DEATH: 09/01/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 09/01/99 14:25 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 500 STUDY WEEK OF DEATH: 72 TERMINAL BODY WEIGHT: 282.0 GRAMS PROSECTOR: JERALD W. JOHNSON RECORDER: JERALD W. JOHNSON PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), KIDNEY (KD), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), HEMATO NEOPLASIA (HN) 3386 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1628 ANIMAL NUMBER: C93030 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/18/00 STUDY DAY OF DEATH: 670 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: 457.8 GRAMS DATE AND TIME OF NECROPSY: 02/18/00 9:45 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; SORE/SCAB; PAW-HINDLEFT; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) KIDNEY (KD) : -LARGE PELVIS(ES); BILATERAL -MASS(ES); LEFT, WITHIN PELVIS: SINGLE MASS, TAN AND FIRM; 15 X 13 X 6 MM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (RENAL) NOT IDENTIFIED ADRENAL, CORTEX (AC) : -SMALL; RIGHT: 5 X 3 X 3 MM (LEFT IS 6 MM IN DIAMETER) HISTOPATHOLOGY BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL -B-ADENOMA, HEPATOCELLULAR,-NEOPLASM PRESENT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SLIGHT -MINERALIZATION, PELVIC,-MODERATE -HYPERPLASIA, TRANSITIONAL CELL,-SLIGHT -B-LIPOMA,-NEOPLASM PRESENT SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-MODERATE -INFILTRATION, FAT, INTERSTITIAL,MODERATE ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATE -PIGMENT,-SLIGHT 3387 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1629 ANIMAL NUMBER: C93030 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/18/00 STUDY DAY OF DEATH: 670 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: 457.8 GRAMS DATE AND TIME OF NECROPSY: 02/18/00 9:45 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); SINGLE MASS, 10 X 5 X 5 MM, RED, DARK RED, TAN, GRAY, AND LOBULATED STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA: MULTIPLE TAN, SLIGHTLY ERODED AREAS, UP TO 2 X 1 MM MAMMARY, FEMALE (MF) : -MASS(ES); MID PERINEUM REGION: SINGLE MASS, 20 X 15 X 10 MM, RED, TAN AND FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (LUMBAR) NORMAL (CAP 21) THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),SLIGHT LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL HEART (HT) : -CARDIOMYOPATHY,-SLIGHT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, NONGL (SU) : -ACANTHOSIS / HYPERKERATOSIS,-MODERATE -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE CECUM (CE) : -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT -B-FIBROADENOMA,-NEOPLASM PRESENT -HYPERPLASIA, GLANDULAR,-SLIGHT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT LN, LUMBAR/ILIAC (LM) : -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL 3388 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1630 ANIMAL NUMBER: C93030 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/18/00 STUDY DAY OF DEATH: 670 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: 457.8 GRAMS DATE AND TIME OF NECROPSY: 02/18/00 9:45 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY FOOT/FOOTPAD (FP) : -ABRASION(S)/ULCERATION(S); LEFT HINDFOOT: SINGLE TAN AND MOIST ABRADED AREA, 3 MM IN DIAMETER; COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN FOOT/FOOTPAD (FP) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE -ULCERATION,-MINIMAL -ACANTHOSIS / HYPERKERATOSIS,-MODERATE -FIBROSIS,-MODERATELY SEVERE 3389 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1631 ANIMAL NUMBER: C93030 SEX: FEMALE DATE OF DEATH: 02/18/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 02/18/00 9:45 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 670 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: 457.8 GRAMS PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) (UB), 3390 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1632 ANIMAL NUMBER: C93031 SEX: FEMALE DATE OF DEATH: 05/15/98 STUDY DAY OF DATE AND TIME OF NECROPSY: 05/15/98 11:46 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 183.6 GRAMS PROSECTOR: LYNN HORNER RECORDER: DEBBIE PIRKEL PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN STATUS LF ADRENAL (AD0) RT ADRENAL (AD1) BRAIN (BR) LF KIDNEY (KD0) RT KIDNEY (KD1) LIVER (LI) LUNG (LU) LF OVARY (OV0) RT OVARY (OV1) SPLEEN (SP) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) UTERUS (UT) ------------------------------------ 7.4393 -------------------------------------------------- ------------------------------------ 4.0511 % -------------------------------------------------- -------------------------------------------------------------------------------------------- NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN WEIGHT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS 3391 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1633 ANIMAL NUMBER: C93031 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 183.6 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 11:46 PROSECTOR: LYNN HORNER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) 3392 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1634 ANIMAL NUMBER: C93032 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/27/99 STUDY DAY OF DEATH: 556 STUDY WEEK OF DEATH: 80 TERMINAL BODY WEIGHT: 284.3 GRAMS DATE AND TIME OF NECROPSY: 10/27/99 7:57 PROSECTOR: JARED FISHEL RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >BEHAVIOR; ATAXIC; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; HUNCHED; VERIFIED AT NECROPSY: NOT APPLICABLE >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -MINERALIZATION,-MINIMAL LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, PELVIC,-MINIMAL -MINERALIZATION, TUBULAR,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MINIMAL PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-SLIGHT -HYPERTROPHY / BASOPHILIA, ACINAR CELL,MINIMAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT -PIGMENT,-MINIMAL 3393 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1635 ANIMAL NUMBER: C93032 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/27/99 STUDY DAY OF DEATH: 556 STUDY WEEK OF DEATH: 80 TERMINAL BODY WEIGHT: 284.3 GRAMS DATE AND TIME OF NECROPSY: 10/27/99 7:57 PROSECTOR: JARED FISHEL RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 12 X 7 X 7 MM, DARK RED, TAN, GRAY, SOFT, AND LOBULATED UTERUS (UT) : -CYST(S); RIGHT HORN, WITHIN WALL: TWO CLEAR FLUID FILLED CYSTS, EACH IS 2 MM IN DIAMETER GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT -HYPERPLASIA, GLANDULAR,-MODERATE UTERUS (UT) : >UNREMARKABLE VAGINA (VA) : -LUMEN, EXUDATE,-PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 3394 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1636 ANIMAL NUMBER: C93032 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/27/99 STUDY DAY OF DEATH: 556 STUDY WEEK OF DEATH: 80 TERMINAL BODY WEIGHT: 284.3 GRAMS DATE AND TIME OF NECROPSY: 10/27/99 7:57 PROSECTOR: JARED FISHEL RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), HEMATO NEOPLASIA (HN) (SG), 3395 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1637 ANIMAL NUMBER: C93033 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/19/99 STUDY DAY OF DEATH: 456 STUDY WEEK OF DEATH: 66 TERMINAL BODY WEIGHT: 211.1 GRAMS DATE AND TIME OF NECROPSY: 07/19/99 15:30 PROSECTOR: STEVE VAN ADESTINE RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); AXILLARY REGION-LEFT; WEEK 45; 10 X 10MM; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; HEAD TILT; RIGHT; VERIFIED AT NECROPSY: NOT APPLICABLE >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; HUNCHED; VERIFIED AT NECROPSY: NOT APPLICABLE >DISCHARGE; CLEAR ORAL; MODERATE; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LUNG (LU) : -MOTTLED; ALL LOBES: RED AND TAN HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-MODERATE -MINERALIZATION, TUBULAR,-MINIMAL -HYPERPLASIA, TRANSITIONAL CELL,-SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-MODERATE ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MINIMAL -HYPERTROPHY, CORTICAL CELL,-MINIMAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATELY SEVERE LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,SLIGHT 3396 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1638 ANIMAL NUMBER: C93033 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/19/99 STUDY DAY OF DEATH: 456 STUDY WEEK OF DEATH: 66 TERMINAL BODY WEIGHT: 211.1 GRAMS DATE AND TIME OF NECROPSY: 07/19/99 15:30 PROSECTOR: STEVE VAN ADESTINE RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 14 X 10 X 8 MM, DARK RED, BROWN, AND SOFT STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: SINGLE DARK BROWN AREA, 2 X 1 MM MAMMARY, FEMALE (MF) : -MASS(ES); LEFT AXILLARY REGION: SINGLE MASS, 18 X 12 X 5 MM, TAN AND FIRM; SAME ON CUT SURFACE (CAP 20); REGIONAL LN (SEE AXILLARY) -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION -MASS #2; RIGHT AXILLARY REGION: SINGLE MASS, 15 X 10 X 5 MM, TAN AND FIRM; SAME ON CUT SURFACE (CAP 21); REGIONAL LN (SEE AXILLARY) -CYST(S); ALL REGIONS, DIFFUSE: MULTIPLE TAN CREAMY SEMIFLUID FILLED CYSTS, PINPOINT AND UP TO 2 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION LN, AXILLARY (AX) : -MOTTLED; BILATERAL:: BROWN AND TAN (CAP 22) LUNG (LU) : -INFLAMMATION, GRANULOMATOUS,-SLIGHT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : >UNREMARKABLE MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT -B-FIBROADENOMA,- 2 NEOPLASMS PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT LN, AXILLARY (AX) : -HYPERPLASIA, LYMPHOPLASMACYTIC,-SLIGHT 3397 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1639 ANIMAL NUMBER: C93033 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/19/99 STUDY DAY OF DEATH: 456 STUDY WEEK OF DEATH: 66 TERMINAL BODY WEIGHT: 211.1 GRAMS DATE AND TIME OF NECROPSY: 07/19/99 15:30 PROSECTOR: STEVE VAN ADESTINE RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN -STAINS-PERINEUM/PERIANAL; PERINEUM: YELLOW AND MOIST -GI TRACT-LARGE LUMEN; ESOPHAGUS, DIFFUSE: UP TO 10 MM IN DIAMETER -DISCHARGE-ORAL; PINK FLUID 3398 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1640 ANIMAL NUMBER: C93033 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/19/99 STUDY DAY OF DEATH: 456 STUDY WEEK OF DEATH: 66 TERMINAL BODY WEIGHT: 211.1 GRAMS DATE AND TIME OF NECROPSY: 07/19/99 15:30 PROSECTOR: STEVE VAN ADESTINE RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS) FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL) URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) (SN), 3399 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1641 ANIMAL NUMBER: C93034 SEX: FEMALE DATE OF DEATH: 11/15/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 11/15/99 14:08 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DEATH: 575 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: NOT ENTERED PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); AXILLARY REGION-LEFT; WEEK 72; 15 X 15MM; VERIFIED AT NECROPSY: NOT OBSERVED >MASS(ES); INGUINAL REGION-LEFT; WEEK 73; 80 X 70MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); AXILLARY REGION-RIGHT; WEEK 73; 10 X 10MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); INGUINAL REGION-RIGHT; WEEK 73; VERIFIED AT NECROPSY: NOT OBSERVED >SKIN & PELAGE; SORE/SCAB; VENTRALABDOMINAL-LEFT; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY EYE (EY) : >NOTE:>TRIMMING COMMENT: ONE EYE MISSING AT TIME OF TRIMMING BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) ADRENAL, CORTEX (AC) : -LARGE; BILATERAL: EACH IS 8 X 6 X 5 MM HISTOPATHOLOGY EYE (EY) : -UNILATERALLY EXAMINED,-PRESENT BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -NECROSIS, COAGULATIVE,-MODERATE -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, PERIPORTAL,-MODERATELY SEVERE KIDNEY (KD) : -MINERALIZATION, PELVIC,-SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATELY SEVERE LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL 3400 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1642 ANIMAL NUMBER: C93034 SEX: FEMALE DATE OF DEATH: 11/15/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 11/15/99 14:08 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DEATH: 575 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: NOT ENTERED PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 7 MM IN DIAMETER, RED, DARK RED, TAN AND FIRM MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT AXILLARY REGION: SINGLE MASS, 12 X 12 X 9 MM, RED, TAN AND FIRM; ON SECTION, EXUDES A TAN CREAMY SEMIFLUID (CAP 22); REGIONAL LN (AXILLARY) NORMAL (CAP 21) -MASS #2; LEFT INGUINAL REGION: SINGLE MASS, 95 X 69 X 34 MM, RED, TAN, DARK RED, VASCULAR AND SOFT; SAME ON CUT SURFACE; COLLECTED INTACT; OVERLYING SKIN HAS A DARK RED MOIST ULCERATED AREA, 30 X 20 MM, COLLECTED INTACT WITH MASS; REGIONAL LN (INGUINAL) NOT IDENTIFIED HEART (HT) : -METAPLASIA, OSSEOUS / CARTILAGINOUS MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT DUODENUM (DU) : >AUTOLYTIC JEJUNUM (JE) : >AUTOLYTIC ILEUM (IL) : >AUTOLYTIC CECUM (CE) : >AUTOLYTIC MAMMARY, FEMALE (MF) : -B-FIBROADENOMA,-NEOPLASM PRESENT -B-ADENOMA,-NEOPLASM PRESENT >NOTE:>THE FIBROADENOMA IS MASS #2 3401 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1643 ANIMAL NUMBER: C93034 SEX: FEMALE DATE OF DEATH: 11/15/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 11/15/99 14:08 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DEATH: 575 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: NOT ENTERED PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY CAUSE OF DEATH (CD) : -NEOPLASM, MAMMARY GLAND,-PRESENT LN, AXILLARY (AX) : -HYPERPLASIA, LYMPHOPLASMACYTIC,-SLIGHT THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG), GENERAL COMMENT (GC) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 3402 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1644 ANIMAL NUMBER: C93035 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 276.6 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 13:36 PROSECTOR: JODI THIEREN RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) LF ADRENAL (AD0) RT ADRENAL (AD1) BRAIN (BR) LF KIDNEY (KD0) RT KIDNEY (KD1) LIVER (LI) LUNG (LU) LF OVARY (OV0) RT OVARY (OV1) SPLEEN (SP) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) UTERUS (UT) .0344 .0292 1.9688 1.2399 1.2468 8.2187 1.2172 .0804 .0836 .5892 .0051 .0094 1.4107 .0124 % .0106 % .7118 % .4483 % .4508 % 2.9713 % .4401 % .0291 % .0302 % .2130 % .0018 % .0034 % .5100 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0175 .0148 1.0000 .6298 .6333 4.1745 .6182 .0408 .0425 .2993 .0026 .0048 .7165 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED UTERUS (UT) : -LUMEN FILLED WITH FLUID; BILATERAL: CLEAR FLUID LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL KIDNEY (KD) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL UTERUS (UT) : >NOT REQUIRED TO BE EXAMINED FOR ANIMAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 3403 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1645 ANIMAL NUMBER: C93035 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 276.6 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 13:36 PROSECTOR: JODI THIEREN RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV) 3404 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1646 ANIMAL NUMBER: C93036 SEX: FEMALE DATE OF DEATH: 12/02/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 12/02/99 15:05 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DEATH: 592 STUDY WEEK OF DEATH: 85 TERMINAL BODY WEIGHT: NOT ENTERED PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; OBES; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK 83; 20 X 20MM; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; TREMORS; ENTIRE BODY; VERIFIED AT NECROPSY: NOT APPLICABLE >RESPIRATION; AUDIBLE; VERIFIED AT NECROPSY: NOT APPLICABLE >RESPIRATION; IRREGULAR; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; RECUMBENT; DORSAL; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; CONVULSIONS; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 8 X 6 X 6 MM, RED, DARK RED, GREY AND SOFT HISTOPATHOLOGY BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-SLIGHT -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, PELVIC,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATELY SEVERE -PIGMENT,-MINIMAL THYMUS (TH) : -HEMORRHAGE,-PRESENT THYROID (TY) : -HYPERPLASIA, C-CELL,-SLIGHT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT 3405 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1647 ANIMAL NUMBER: C93036 SEX: FEMALE DATE OF DEATH: 12/02/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 12/02/99 15:05 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DEATH: 592 STUDY WEEK OF DEATH: 85 TERMINAL BODY WEIGHT: NOT ENTERED PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS(ES); LEFT INGUINAL REGION: SINGLE MASS, 35 X 28 X 18 MM, RED, TAN, FIRM AND LOBULATED; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION -CYST(S); ALL REGIONS, DIFFUSE: MULTIPLE TAN CREAMY FLUID FILLED CYSTS, UP TO 8 MM IN DIAMETER; COLLECTED VAGINA (VA) : -SEMIFLUID MATERIAL; WITHIN LUMEN: LIGHT GREEN LN, MANDIBULAR (MN) : -MOTTLED; BILATERAL: RED AND TAN; COLLECTED INTACT WITH MANDIBULAR SALIVARY GLANDS GENERAL COMMENT (GC) : -ANIMAL OBESE MAMMARY, FEMALE (MF) : -M-CARCINOMA,-NEOPLASM PRESENT -CYST / GALACTOCELE,-PRESENT -HYPERPLASIA, GLANDULAR,-MODERATELY SEVERE CERVIX (CV) : -HYPERPLASIA, MUCINOUS,-SLIGHT VAGINA (VA) : -HYPERPLASIA, MUCINOUS,-MODERATELY SEVERE CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT LN, MANDIBULAR (MN) : -HYPERPLASIA, LYMPHOPLASMACYTIC,MODERATE -HEMORRHAGE,-PRESENT 3406 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1648 ANIMAL NUMBER: C93036 SEX: FEMALE DATE OF DEATH: 12/02/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 12/02/99 15:05 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DEATH: 592 STUDY WEEK OF DEATH: 85 TERMINAL BODY WEIGHT: NOT ENTERED PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MEs /pANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), HEMATO NEOPLASIA (HN) 3407 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1649 ANIMAL NUMBER: C93037 SEX: FEMALE DATE OF DEATH: 07/22/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 07/22/99 8:05 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 459 STUDY WEEK OF DEATH: 66 TERMINAL BODY WEIGHT: 370.4 GRAMS PROSECTOR: LYNN HORNER RECORDER: KEVIN BILLINGS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); VENTRAL-CERVICAL-LEFT; WEEK 64; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; NO FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; HEAD TILT; LEFT; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; CIRCLING; LEFT; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) HISTOPATHOLOGY BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-SLIGHT KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-MODERATE ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT -DEGENERATION, CYSTIC / HEMORRHAGIC,MINIMAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY), MINIMAL LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT 3408 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1650 ANIMAL NUMBER: C93037 SEX: FEMALE DATE OF DEATH: 07/22/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 07/22/99 8:05 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 459 STUDY WEEK OF DEATH: 66 TERMINAL BODY WEIGHT: 370.4 GRAMS PROSECTOR: LYNN HORNER RECORDER: KEVIN BILLINGS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 16 X 12 X 7 MM, RED, DARK RED, GRAY, LOBULATED AND SOFT MAMMARY, FEMALE (MF) : -CYST(S); ALL REGIONS, DIFFUSE: MULTIPLE TAN, CREAMY SEMIFLUID FILLED CYSTS, UP TO 15 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION; LARGEST CYSTS ARE POSSIBLY CONSISTENT WITH MASS NOTED IN CLINICAL OBSERVATIONS (CAP 20) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; RIGHT LOWER INCISOR -DISCHARGE-OCULAR; BILATERAL: DARK RED DARK AND DRY HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT VAGINA (VA) : -LUMEN, EXUDATE,-PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 3409 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1651 ANIMAL NUMBER: C93037 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/22/99 STUDY DAY OF DEATH: 459 STUDY WEEK OF DEATH: 66 TERMINAL BODY WEIGHT: 370.4 GRAMS DATE AND TIME OF NECROPSY: 07/22/99 8:05 PROSECTOR: LYNN HORNER RECORDER: KEVIN BILLINGS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), HEMATO NEOPLASIA (HN) (SN), 3410 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1652 ANIMAL NUMBER: C93038 SEX: FEMALE DATE OF DEATH: 08/23/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 08/23/99 10:57 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 491 STUDY WEEK OF DEATH: 71 TERMINAL BODY WEIGHT: 553.5 GRAMS PROSECTOR: DAVID SCHUETTE RECORDER: JARED FISHEL PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); AXILLARY REGION- RIGHT; WEEK 53; 115 X 115MM; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY PITUITARY (PI) : -LARGE; 6 X 5 X 4 MM HISTOPATHOLOGY LIVER (LI) : -HYPERTROPHY, HEPATOCELLULAR PERIPORTAL,- MODERATE KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, PELVIC,-MINIMAL -MINERALIZATION, TUBULAR,-MINIMAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MINIMAL PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-MODERATE ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT -PIGMENT,-SLIGHT LN, MESENTERIC (MS) : -HYPERPLASIA, RETICULOENDOTHELIAL,SLIGHT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATE PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT 3411 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1653 ANIMAL NUMBER: C93038 SEX: FEMALE DATE OF DEATH: 08/23/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 08/23/99 10:57 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 491 STUDY WEEK OF DEATH: 71 TERMINAL BODY WEIGHT: 553.5 GRAMS PROSECTOR: DAVID SCHUETTE RECORDER: JARED FISHEL PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MOTTLED; BROWN, TAN AND DARK RED MAMMARY, FEMALE (MF) : -MASS(ES); LEFT LATERAL THORACIC REGION: SINGLE MASS, 110 X 85 X 55 MM (292.5 GRAMS), TAN, RED, SOFT, LOBULATED AND VASCULAR; SAME ON CUT SURFACE; PORTION COLLECTED; REGIONAL LN (AXILLARY) NORMAL (CAP 20) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN GENERAL INFORMATION (XX) : >NOTE:>ANIMAL INADVERTENTLY SACRIFICED WITH SODIUM PENTOBARBITAL SKIN (SK) : -B-FIBROMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT >NOTE:>SEE SKIN FOR DIAGNOSIS UTERUS (UT) : -CYST,-SLIGHT CAUSE OF DEATH (CD) : -NEOPLASM, SKIN/MUSCLE/SUBCUTIS,PRESENT LN, AXILLARY (AX) : -HYPERPLASIA, LYMPHOPLASMACYTIC,-SLIGHT 3412 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1654 ANIMAL NUMBER: C93038 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/23/99 STUDY DAY OF DEATH: 491 STUDY WEEK OF DEATH: 71 TERMINAL BODY WEIGHT: 553.5 GRAMS DATE AND TIME OF NECROPSY: 08/23/99 10:57 PROSECTOR: DAVID SCHUETTE RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), ADRENAL, MEDULLA (MA), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) (SE), 3413 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1655 ANIMAL NUMBER: C93039 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/07/00 STUDY DAY OF DEATH: 719 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 587.2 GRAMS DATE AND TIME OF NECROPSY: 04/07/00 7:24 PROSECTOR: JARED FISHEL RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); INGUINAL REGION-LEFT; WEEK 66; 70 X 60MM; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE DARK RED AREAS, UP TO 4 MM IN DIAMETER; COLLECTED ON ROUTINE SECTIONS OVARY (OV) : -CYST(S); RIGHT: SINGLE CLEAR FLUID FILLED CYST, 3 MM IN DIAMETER HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MODERATE -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, PELVIC,-MINIMAL -INFLAMMATION, SUPPURATIVE, PELVIC,MINIMAL PANCREAS (PA) : -INFILTRATION, FAT, INTERSTITIAL,SLIGHT OVARY (OV) : -UNILATERALLY EXAMINED,-PRESENT LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL 3414 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1656 ANIMAL NUMBER: C93039 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/07/00 STUDY DAY OF DEATH: 719 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 587.2 GRAMS DATE AND TIME OF NECROPSY: 04/07/00 7:24 PROSECTOR: JARED FISHEL RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 9 X 7 X 5 MM, RED, TAN, AND SOFT SKIN (SK) : >NOTE:>SKIN ROUTINE SECTION TAKEN FROM RIGHT INGUINAL REGION DUE TO MAMMARY MASS MAMMARY, FEMALE (MF) : -MASS(ES); LEFT INGUINAL REGION: SINGLE MASS, 80 X 70 X 40 MM, RED, TAN, FIRM, AND LOBULATED; SAME ON CUT SURFACE; PORTION COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED >NOTE:>MAMMARY ROUTINE SECTION TAKEN FROM RIGHT INGUINAL REGION DUE TO MAMMARY MASS UTERUS (UT) : -CYST(S); BILATERAL WITHIN WALLS: MULTIPLE CLEAR FLUID FILLED CYSTS, UP TO 4 MM IN DIAMETER GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT SKIN (SK) : >NOT REQUIRED TO BE EXAMINED FOR ANIMAL MAMMARY, FEMALE (MF) : -B-FIBROADENOMA,-NEOPLASM PRESENT -HYPERPLASIA, GLANDULAR,-MINIMAL UTERUS (UT) : -HYPERPLASIA, CYSTIC, ENDOMETRIAL,MODERATE CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 3415 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1657 ANIMAL NUMBER: C93039 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/07/00 STUDY DAY OF DEATH: 719 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 587.2 GRAMS DATE AND TIME OF NECROPSY: 04/07/00 7:24 PROSECTOR: JARED FISHEL RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PARATHYROID (PT), URINARY BLADDER (UB) 3416 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1658 ANIMAL NUMBER: C93040 SEX: FEMALE DATE OF DEATH: 04/07/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/07/00 7:50 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 719 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 296.4 GRAMS PROSECTOR: JAMES T. CALLEN RECORDER: TONE EVERTS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); AXILLARY REGION-RIGHT; WEEK 76; 20 X 15MM; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; ROUGH HAIR COAT; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; ATAXIC; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 12 X 8 X 6 MM, DARK RED, TAN, AND FIRM MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT AXILLARY REGION: SINGLE MASS, 28 X 23 X 14 MM, RED, TAN, LOBULATED, AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (AXILLARY) NORMAL (CAP 22) HISTOPATHOLOGY BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, PERIPORTAL,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, PELVIC,-SLIGHT -HYPERPLASIA, TRANSITIONAL CELL,-SLIGHT THYROID (TY) : -HYPERPLASIA, C-CELL,-MODERATE PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT -B-FIBROADENOMA,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 3417 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1659 ANIMAL NUMBER: C93040 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/07/00 STUDY DAY OF DEATH: 719 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 296.4 GRAMS DATE AND TIME OF NECROPSY: 04/07/00 7:50 PROSECTOR: JAMES T. CALLEN RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -DISCHARGE-OCULAR; BILATERAL: MATERIAL -ANIMAL THIN DRY RED THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), LUNG (LU), PARATHYROID (PT), URINARY BLADDER (UB), LN, AXILLARY (AX) 3418 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1660 ANIMAL NUMBER: C93041 SEX: FEMALE DATE OF DEATH: 11/15/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 11/15/99 13:20 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 575 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: 375.9 GRAMS PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); AXILLARY REGION-LEFT; WEEK 73; 10 X 10MM; VERIFIED AT NECROPSY: NOT OBSERVED >SKIN & PELAGE; RED HAIR COAT; LATERALTHORACIC-LEFT; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; RED HAIR COAT; LATERARIGHT; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); AXILLARY REGION-RIGHT; WEEK 82; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; HEAD TILT; LEFT; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; ATAXIC; VERIFIED AT NECROPSY: NOT APPLICABLE >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 11 X 9 X 5 MM, DARK RED, RED AND SOFT HISTOPATHOLOGY BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-SEVERE -HYPERPLASIA, TRANSITIONAL CELL,MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MINIMAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATE -HYPERTROPHY, CORTICAL CELL,-MINIMAL -PIGMENT,-SLIGHT HEART (HT) : -CARDIOMYOPATHY,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT 3419 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1661 ANIMAL NUMBER: C93041 SEX: FEMALE DATE OF DEATH: 11/15/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 11/15/99 13:20 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 575 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: 375.9 GRAMS PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT AXILLARY REGION: SINGLE MASS, 10 MM IN DIAMETER, TAN, BROWN, RED AND FIRM; SAME ON CUT SURFACE, COLLECTED INTACT; REGIONAL LN (AXILLARY) NORMAL (CAP 21) UTERUS (UT) : -LUMEN FILLED WITH FLUID; BILATERAL: CLEAR FLUID -CYST(S); BILATERAL, WITHIN WALLS: MULTIPLE CLEAR FLUID FILLED CYSTS, UP TO 3 MM IN DIAMETER GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -STAINS - SKIN/HAIRCOAT; ENTIRE ABDOMINAL AND DORSAL THORACIC REGIONS: DRY RED MATERIAL MAMMARY, FEMALE (MF) : -M-CARCINOMA,-NEOPLASM PRESENT -CYST / GALACTOCELE,-PRESENT -HYPERPLASIA, GLANDULAR,-MINIMAL UTERUS (UT) : -DILATATION,-SEVERE VAGINA (VA) : -HYPERPLASIA, MUCINOUS,-SLIGHT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT LN, AXILLARY (AX) : -HYPERPLASIA, LYMPHOPLASMACYTIC,MINIMAL 3420 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1662 ANIMAL NUMBER: C93041 SEX: FEMALE DATE OF DEATH: 11/15/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 11/15/99 13:20 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 575 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: 375.9 GRAMS PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON(CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUm /s KULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), HEMATO NEOPLASIA (HN) 3421 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1663 ANIMAL NUMBER: C93042 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/13/00 STUDY DAY OF DEATH: 665 STUDY WEEK OF DEATH: 95 TERMINAL BODY WEIGHT: 394.0 GRAMS DATE AND TIME OF NECROPSY: 02/13/00 13:55 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); INGUINAL REGION-LEFT; WEEK 79; 70 X 50MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); INGUINAL REGION-RIGHT; WEEK 79; 10 X 10MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); AXILLARY REGION-LEFT; WEEK 82; 10 X 10MM; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; AXILLARY REGION-LEFT; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); DORSAL; WEEK 92; 30 X 30MM; VERIFIED AT NECROPSY: OBSERVED >RESPIRATION; AUDIBLE; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -CYST(S); VISCERAL SURFACE OF LEFT LATERAL LOBE: SINGLE CLEAR FLUID FILLED CYST, 1 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION ADRENAL, CORTEX (AC) : -RED FOCUS(I)/AREAS(I); RIGHT: TWO DARK RED AND SLIGHTLY RAISED AREAS, 3 X 2 X 1 MM HISTOPATHOLOGY BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT -I-CARCINOMA,-FROM PITUITARY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL -FIBROSIS, PERIBILIARY,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, PELVIC,-SLIGHT SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE PANCREAS (PA) : -ATROPHY, ACINAR,-MINIMAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATE -PIGMENT,-SLIGHT -B-ADENOMA,-NEOPLASM PRESENT OVARY (OV) : -CYST,-PRESENT 3422 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1664 ANIMAL NUMBER: C93042 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/13/00 STUDY DAY OF DEATH: 665 STUDY WEEK OF DEATH: 95 TERMINAL BODY WEIGHT: 394.0 GRAMS DATE AND TIME OF NECROPSY: 02/13/00 13:55 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY OVARY (OV) : -CYST(S); BILATERAL: SINGLE CLEAR FLUID FILLED CYST ON EACH, EACH IS 3 MM IN DIAMETER PITUITARY (PI) : -MASS(ES); 10 X 7 X 6 MM, DARK RED, DARK BROWN, TAN AND SOFT MAMMARY, FEMALE (MF) : -MASS(ES); LEFT AXILLARY REGION: SINGLE MASS, 15 X 15 X 8 MM, DARK RED, TAN, LOBULATED AND FIRM; SAME ON CUT SURFACE (CAP 21); OVERLYING SKIN HAS A SINGLE TAN AND DRY CRUSTED AREA, 8 X 5 MM; COLLECTED INTACT WITH MASS; REGIONAL LN (AXILLARY) NORMAL (CAP 22) MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,-MODERATE MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,-MODERATE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATE HEART (HT) : -CARDIOMYOPATHY,-SLIGHT THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PITUITARY (PI) : -M-CARCINOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL MAMMARY, FEMALE (MF) : -M-CARCINOMA,- 5 NEOPLASMS PRESENT -CYST / GALACTOCELE,-PRESENT -INFLAMMATION, CHRONIC-ACTIVE,MODERATELY SEVERE -HYPERPLASIA, GLANDULAR,-MODERATE 3423 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1665 ANIMAL NUMBER: C93042 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/13/00 STUDY DAY OF DEATH: 665 STUDY WEEK OF DEATH: 95 TERMINAL BODY WEIGHT: 394.0 GRAMS DATE AND TIME OF NECROPSY: 02/13/00 13:55 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -THICKENED; VENTRAL CERVICAL REGION: TAN AND FIRM (CAP 23) -MASS #2; LEFT PERINEAL REGION: SINGLE MASS, 70 X 45 X 25 MM, DARK RED, TAN AND FIRM; ON SECTION EXUDES A DARK RED FLUID (CAP 24); REGIONAL LN (SEE INGUINAL) -MASS #3; RIGHT INGUINAL REGION: SINGLE MASS, 25 X 25 X 15 MM, TAN, LOBULATED AND FIRM; SOME SECTIONS EXUDE A TAN AND CREAMY SEMI- FLUID (CAP 25); REGIONAL LN (SEE INGUINAL) -MASS #4; RIGHT INGUINAL REGION, CRANIAL TO MASS #3: SINGLE MASS, 17 X 14 X 9 MM, TAN AND FIRM; SAME ON CUT SURFACE (CAP 27); REGIONAL LN (SEE INGUINAL) -MASS #5; DORSAL THORACIC REGION: SINGLE MASS, 15 MM IN DIAMETER, DARK RED, BROWN AND FIRM; ON SECTION EXUDES A DARK BROWN FLUID (CAP 28); REGIONAL LN (AXILLARY) NORMAL (CAP 22) UTERUS (UT) : -HYPERPLASIA, CYSTIC, ENDOMETRIAL,MINIMAL CERVIX (CV) : -EXUDATE, LUMINAL,-PRESENT 3424 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1666 ANIMAL NUMBER: C93042 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/13/00 STUDY DAY OF DEATH: 665 STUDY WEEK OF DEATH: 95 TERMINAL BODY WEIGHT: 394.0 GRAMS DATE AND TIME OF NECROPSY: 02/13/00 13:55 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY LN, INGUINAL (IN) : -LARGE; LEFT: UP TO 12 X 8 X 7 MM; RIGHT IS NORMAL (CAP 26) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN VAGINA (VA) : -LUMEN, EXUDATE,-PRESENT -HYPERPLASIA, MUCINOUS,-SLIGHT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT LN, AXILLARY (AX) : -HYPERPLASIA, LYMPHOPLASMACYTIC,MODERATELY SEVERE LN, INGUINAL (IN) : >UNREMARKABLE >NOTE:>MAMMARY NEOPLASM ALSO ON SLIDE 3425 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1667 ANIMAL NUMBER: C93042 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/13/00 STUDY DAY OF DEATH: 665 STUDY WEEK OF DEATH: 95 TERMINAL BODY WEIGHT: 394.0 GRAMS DATE AND TIME OF NECROPSY: 02/13/00 13:55 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), HEMATO NEOPLASIA (HN) 3426 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1668 ANIMAL NUMBER: C93043 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/21/00 STUDY DAY OF DEATH: 673 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 620.1 GRAMS DATE AND TIME OF NECROPSY: 02/21/00 14:27 PROSECTOR: JODI THIEREN RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); AXILLARY REGION-RIGHT; WEEK 68; 100 X 70MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); VENTRAL-CERVICAL-LEFT; WEEK 73; 20 X 20MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); VENTRAL-ABDOMINAL; WEEK 83; 40 X 35MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); VENTRAL-CERVICAL-RIGHT; WEEK 84; 50 X 55MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); AXILLARY REGION-LEFT; WEEK 95; 15 X 15MM; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; AXILLARY REGION-LEFT; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -LARGE; ALL LOBES: MARGINS APPEAR ROUNDED -RED FOCUS(I)/AREA(S); DIAPHRAGMATIC SURFACE OF RIGHT MEDIAN LOBE: SINGLE DARK RED AREA, 3 X 2 MM; COLLECTED ON ROUTINE SECTION HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, PELVIC,-SLIGHT SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-SLIGHT -ANGIECTASIS,-MODERATE -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL -HYPERTROPHY, CORTICAL CELL,-MODERATELY SEVERE -DEGENERATION, CYSTIC / HEMORRHAGIC,MODERATELY SEVERE 3427 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1669 ANIMAL NUMBER: C93043 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/21/00 STUDY DAY OF DEATH: 673 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 620.1 GRAMS DATE AND TIME OF NECROPSY: 02/21/00 14:27 PROSECTOR: JODI THIEREN RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY LN, MESENTERIC (MS) : -DIFFUSELY DARK; DARK GREEN OVARY (OV) : -CYST(S); BILATERAL: SINGLE CLEAR FLUID FILLED CYST ON EACH, EACH IS 1 MM IN DIAMETER PITUITARY (PI) : -MASS(ES); SINGLE MASS, 8 X 6 X 5 MM, RED, DARK RED, TAN, LOBULATED, AND FIRM ADRENAL, MEDULLA (MA) : -UNILATERALLY EXAMINED,-PRESENT LN, MESENTERIC (MS) : >UNREMARKABLE OVARY (OV) : >UNREMARKABLE MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,MODERATELY SEVERE MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,MODERATELY SEVERE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MINIMAL HEART (HT) : -CARDIOMYOPATHY,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT RECTUM (RE) : -PARASITISM,-PRESENT MAMMARY, FEMALE (MF) : -M-CARCINOMA,- 5 NEOPLASMS PRESENT -CYST / GALACTOCELE,-PRESENT 3428 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1670 ANIMAL NUMBER: C93043 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/21/00 STUDY DAY OF DEATH: 673 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 620.1 GRAMS DATE AND TIME OF NECROPSY: 02/21/00 14:27 PROSECTOR: JODI THIEREN RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS(ES); LEFT VENTRAL CERVICAL REGION: SINGLE MASS, 23 X 22 X 17 MM, RED, DARK RED, BROWN, TAN, LOBULATED, AND SOFT; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (MANDIBULAR) NORMAL (CAP 23) -THICKENED; ALL REGIONS DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION -MASS #2; RIGHT VENTRAL CERVICAL REGION: SINGLE MASS, 65 X 55 X 49 MM, RED, DARK RED, TAN, FIRM, VASCULAR, AND LOBULATED; ON CUT SURFACE EXUDES A BROWN FLUID; PORTION COLLECTED; REGIONAL LN (MANDIBULAR) NORMAL (CAP 23) -MASS #3; RIGHT AXILLARY REGION EXTENDING TO RIGHT INGUINAL REGION: SINGLE MASS, 120 X 75 X 70 MM, RED, TAN, FIRM, LOBULATED, AND VASCULAR; SAME ON CUT SURFACE; PORTION COLLECTED; REGIONAL LN (SEE AXILLARY) -MASS #4; LEFT AXILLARY REGION: SINGLE MASS, 20 X 15 X 11 MM, RED, TAN, AND FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (SEE AXILLARY); OVERLYING SKIN HAS A SINGLE DRY CRUSTED AREA, 4 X 3 MM ;COLLECTED INTACT WITH MASS MAMMARY, FEMALE (MF) : -B-FIBROADENOMA,-NEOPLASM PRESENT -HYPERPLASIA, GLANDULAR,-SLIGHT >NOTE:>MASS #3 IS THE FIBROADENOMA 3429 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1671 ANIMAL NUMBER: C93043 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/21/00 STUDY DAY OF DEATH: 673 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 620.1 GRAMS DATE AND TIME OF NECROPSY: 02/21/00 14:27 PROSECTOR: JODI THIEREN RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS #5; PERINEAL REGION: SINGLE MASS, 43 X 32 X 22 MM, TAN, FIRM, LOBULATED, AND VASCULAR; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (SEE INGUINAL) UTERUS (UT) : -CYST(S); BILATERAL WITHIN WALLS: MULTIPLE CLEAR FLUID FILLED CYSTS, UP TO 17 X 10 X 10 MM -MASS(ES); RIGHT HORN WITHIN WALL: SINGLE MASS, DARK RED AND SOFT, 8 X 5 X 5 MM, SAME ON CUT SURFACE; REGIONAL LN (SEE LUMBAR) LN, AXILLARY (AX) : -LARGE; LEFT: 11 X 7 X 2 MM (RIGHT IS 7 X 4 X 1 MM) (CAP 24) LN, INGUINAL (IN) : -LARGE; RIGHT: 14 X 10 X 10 MM (LEFT NOT IDENTIFIED) (CAP 33) UTERUS (UT) : -HYPERPLASIA, CYSTIC, ENDOMETRIAL,SEVERE CERVIX (CV) : -HYPERPLASIA, MUCINOUS,-SLIGHT VAGINA (VA) : -HYPERPLASIA, MUCINOUS,-SEVERE CAUSE OF DEATH (CD) : -NEOPLASM, MAMMARY GLAND,-PRESENT LN, AXILLARY (AX) : -HYPERPLASIA, LYMPHOPLASMACYTIC,MODERATELY SEVERE LN, INGUINAL (IN) : >TISSUE MISSING >NOTE:>TISSUE IS MAMMARY GLAND WITH CARCINOMA 3430 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1672 ANIMAL NUMBER: C93043 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/21/00 STUDY DAY OF DEATH: 673 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 620.1 GRAMS DATE AND TIME OF NECROPSY: 02/21/00 14:27 PROSECTOR: JODI THIEREN RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY LN, LUMBAR/ILIAC (LM) : -MOTTLED; BILATERAL: RED AND TAN (CAP 34) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN LN, MANDIBULAR (MN) : -HYPERPLASIA, LYMPHOPLASMACYTIC,MODERATE LN, LUMBAR/ILIAC (LM) : -HYPERPLASIA, LYMPHOPLASMACYTIC,MODERATE -HEMORRHAGE,-PRESENT 3431 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1673 ANIMAL NUMBER: C93043 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/21/00 STUDY DAY OF DEATH: 673 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 620.1 GRAMS DATE AND TIME OF NECROPSY: 02/21/00 14:27 PROSECTOR: JODI THIEREN RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM COLON (CO), SKIN (SK), URINARY BLADDER (UB), HEMATO NEOPLASIA (HN) (SM), (CE), 3432 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1674 ANIMAL NUMBER: C93044 SEX: FEMALE DATE OF DEATH: 07/19/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 07/19/99 15:37 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 456 STUDY WEEK OF DEATH: 66 TERMINAL BODY WEIGHT: 308.1 GRAMS PROSECTOR: STEVE VAN ADESTINE RECORDER: LYNN HORNER PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; HEAD TILT; LEFT; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; HEAD-ENTIRE; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; HYPOACTIVE; MODERATE; VERIFIED AT NECROPSY: NOT APPLICABLE >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES) ; 12 X 8 X 7 MM, RED, DARK RED, GRAY, TAN, SOFT AND LOBULATED SKIN (SK) -CRUSTED AREA(S) ; DORSAL SURFACE OF MUZZLE: SINGLE DARK RED, DRY CRUSTED AREA, 3 X 2 MM (CAP 20) HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT -PIGMENT,-MINIMAL OVARY (OV) : -CYST,-PRESENT LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, NONGL (SU) : -ACANTHOSIS / HYPERKERATOSIS,-MINIMAL -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT SKIN (SK) : -ACANTHOSIS/HYPERKERATOSIS,-SLIGHT -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE -SUPERFICIAL CRUSTING,-MODERATELY SEVERE 3433 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1675 ANIMAL NUMBER: C93044 SEX: FEMALE DATE OF DEATH: 07/19/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 07/19/99 15:37 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 456 STUDY WEEK OF DEATH: 66 TERMINAL BODY WEIGHT: 308.1 GRAMS PROSECTOR: STEVE VAN ADESTINE RECORDER: LYNN HORNER PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION -CYST(S); ALL REGIONS, DIFFUSE: MULTIPLE TAN CREAMY SEMIFLUID FILLED CYSTS, PINPOINT AND UP TO 5 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL UPPER INCISORS -DISCHARGE-OCULAR; BILATERAL: DARK RED, DRY, CRUSTED MATERIAL -ANIMAL THIN MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT -HYPERPLASIA, GLANDULAR,-MODERATE -B-ADENOMA,-NEOPLASM PRESENT -INFLAMMATION, CHRONIC,-MODERATE CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 3434 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1676 ANIMAL NUMBER: C93044 SEX: FEMALE DATE OF DEATH: 07/19/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 07/19/99 15:37 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 456 STUDY WEEK OF DEATH: 66 TERMINAL BODY WEIGHT: 308.1 GRAMS PROSECTOR: STEVE VAN ADESTINE RECORDER: LYNN HORNER PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), KIDNEY (KD), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) (CE), 3435 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1677 ANIMAL NUMBER: C93045 SEX: FEMALE DATE OF DEATH: 05/15/98 STUDY DAY OF DATE AND TIME OF NECROPSY: 05/15/98 11:15 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 221.5 GRAMS PROSECTOR: LYNN HORNER RECORDER: DEBBIE PIRKEL PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN STATUS LF ADRENAL (AD0) RT ADRENAL (AD1) BRAIN (BR) LF KIDNEY (KD0) RT KIDNEY (KD1) LIVER (LI) LUNG (LU) LF OVARY (OV0) RT OVARY (OV1) SPLEEN (SP) LF THYROID/PARA RT THYROID/PARA UTERUS (UT) (TT0) (TT1) -------- 8.6925 -------- -------- -------- -------- 3.9251 % -------- -------- -------- -------- --------------- -------- -------- NOT TAKEN WEIGHT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS NOTTAK NOTTAK NOTTAK NOTTAK NOTTAK NOTTAK NOTTAK NOTTAK 3436 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1678 ANIMAL NUMBER: C93045 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 221.5 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 11:15 PROSECTOR: LYNN HORNER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) 3437 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1679 ANIMAL NUMBER: C93046 SEX: FEMALE DATE OF DEATH: 10/25/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 10/25/99 13:59 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 554 STUDY WEEK OF DEATH: 80 TERMINAL BODY WEIGHT: 500.4 GRAMS PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); INGUINAL REGION-RIGHT; WEEK 58; 90 X 90MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); VENTRAL-CERVICAL-LEFT; WEEK 64; 30 X 25MM; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; VENTRALCERVICAL-LEFT; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); INGUINAL REGION-LEFT; WEEK 73; 10 X 10MM; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; PROLAPSED; VAGINA; VERIFIED AT NECROPSY: OBSERVED >DISCHARGE; GENITAL; RED IN COLOR; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; COLD TO TOUCH; ENTIRE BODY; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY HISTOPATHOLOGY LIVER (LI) : -HYPERTROPHY, HEPATOCELLULAR, PERIPORTAL,-MODERATE -MITOSIS, HEPATOCELLULAR, INCREASED,MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, PELVIC,-MINIMAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SLIGHT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY) MINIMAL PITUITARY (PI) : -HYPERPLASIA,-SLIGHT MAMMARY, FEMALE (MF) : -M-CARCINOMA,-NEOPLASM PRESENT -B-FIBROADENOMA,- 3 NEOPLASMS PRESENT -HYPERPLASIA, GLANDULAR,-SLIGHT >NOTE:>MASS #1 CONTAINS BOTH FIBROADENOMA AND CARCINOMA 3438 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1680 ANIMAL NUMBER: C93046 SEX: FEMALE DATE OF DEATH: 10/25/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 10/25/99 13:59 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 554 STUDY WEEK OF DEATH: 80 TERMINAL BODY WEIGHT: 500.4 GRAMS PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS(ES); LEFT VENTRAL CERVICAL REGION: SINGLE MASS, 41 X 25 X 20 MM, RED, DARK RED, GREY, TAN, LOBULATED AND SOFT; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (MANDIBULAR) NORMAL (CAP 21); OVERLYING SKIN HAS A SINGLE DRY, BROWN CRUSTED AREA, 7 X 4 MM; COLLECTED WITH MASS -MASS #2; RIGHT INGUINAL REGION: SINGLE MASS, 100 X 80 X 36 MM, RED, GREY, TAN, LOBULATED, VASCULAR AND FIRM; SOME SECTIONS EXUDE A GREEN CASEOUS MATERIAL; COLLECTED INTACT; REGIONAL LN (INGUINAL) NOT IDENTIFIED -MASS #3; LEFT INGUINAL REGION: SINGLE MASS, 12 X 10 X 5 MM, RED, GREY, TAN AND FIRM; SAME ON CUT SURFACE (CAP 22); REGIONAL LN (INGUINAL) NOT IDENTIFIED UTERUS (UT) : -THICKENED WALL; BILATERAL, DIFFUSE UTERUS (UT) : -HYPERPLASIA, CYSTIC, ENDOMETRIAL SLIGHT VAGINA (VA) : -B-POLYP, STROMAL,-NEOPLASM PRESENT 3439 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1681 ANIMAL NUMBER: C93046 SEX: FEMALE DATE OF DEATH: 10/25/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 10/25/99 13:59 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 554 STUDY WEEK OF DEATH: 80 TERMINAL BODY WEIGHT: 500.4 GRAMS PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY VAGINA (VA) : -MASS(ES); WITHIN LUMEN: SINGLE MASS, 32 X 12 X 12 MM, DARK RED, GELATINOUS AND SOFT; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (LUMBAR) NORMAL (CAP 23); POSSIBLY CONSISTENT WITH PROLAPSE (VAGINA) AS NOTED IN CLINICAL OBSERVATIONS GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -STAINS-PERINEUM/PERIANAL; PERINEUM REGION: DARK RED AND MOIST MATERIAL -GI TRACT-DARK FLUID CONTENT; STOMACH: BLACK SEMIFLUID MATERIAL CAUSE OF DEATH (CD) : -NEOPLASM, MAMMARY GLAND,-PRESENT >NOTE:>THE FIBROADENOMA IS THE CAUSE OF DEATH LN, MANDIBULAR (MN) : -HYPERPLASIA, LYMPHOPLASMACYTIC,MINIMAL LN, LUMBAR/ILIAC (LM) : -HYPERPLASIA, LYMPHOPLASMACYTIC,-SLIGHT 3440 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1682 ANIMAL NUMBER: C93046 SEX: FEMALE DATE OF DEATH: 10/25/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 10/25/99 13:59 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 554 STUDY WEEK OF DEATH: 80 TERMINAL BODY WEIGHT: 500.4 GRAMS PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), HEMATO NEOPLASIA (HN) 3441 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1683 ANIMAL NUMBER: C93047 SEX: FEMALE DATE OF DEATH: 04/07/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/07/00 7:52 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 719 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 864.4 GRAMS PROSECTOR: MICHELE MARGGI RECORDER: JILL PAUS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; OBES; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -LIGHT FOCUS(I)/AREA(S); DIAPHRAGMATIC SURFACE OF LEFT LATERAL LOBE: SINGLE TAN AREA, 4 X 3 MM; COLLECTED ON ROUTINE SECTION -RED FOCUS(I)/AREA(S); RIGHT MEDIAN LOBE: MULTIPLE DARK RED AREAS, UP TO 3 X 2 MM; COLLECTED ON ROUTINE SECTION HISTOPATHOLOGY BRAIN (BR) : >UNREMARKABLE LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MODERATE -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, PELVIC,-MINIMAL PANCREAS (PA) : -ATROPHY, ACINAR,-MODERATELY SEVERE -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT 3442 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1684 ANIMAL NUMBER: C93047 SEX: FEMALE DATE OF DEATH: 04/07/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/07/00 7:52 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 719 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 864.4 GRAMS PROSECTOR: MICHELE MARGGI RECORDER: JILL PAUS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 7 X 6 X 5 MM, DARK RED, GRAY, TAN AND SOFT STOMACH, GL (ST) : -LIGHT FOCUS(I)/AREA(S); MUCOSA: MULTIPLE TAN AREAS, UP TO 4 X 3 MM MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT ABDOMINAL REGION: SINGLE MASS, 30 X 25 X 15 MM, RED, TAN, VASCULAR AND FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (LUMBAR) NORMAL (CAP 22) -THICKENED; ALL REGIONS DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -ANIMAL OBESE PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : -INFLAMMATION, ACUTE,-SLIGHT -EROSION,-MINIMAL MAMMARY, FEMALE (MF) : -M-CARCINOMA,-NEOPLASM PRESENT -HYPERPLASIA, GLANDULAR,-MODERATE CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, LUMBAR/ILIAC (LM) : -HYPERPLASIA, LYMPHOPLASMACYTIC,MINIMAL 3443 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1685 ANIMAL NUMBER: C93047 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/07/00 STUDY DAY OF DEATH: 719 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 864.4 GRAMS DATE AND TIME OF NECROPSY: 04/07/00 7:52 PROSECTOR: MICHELE MARGGI RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PARATHYROID (PT), URINARY BLADDER (UB) 3444 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1686 ANIMAL NUMBER: C93048 SEX: FEMALE DATE OF DEATH: 04/07/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/07/00 7:52 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 719 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 445.2 GRAMS PROSECTOR: GREGORY CODDINGTON RECORDER: TONE EVERTS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY CERVIX (CV) : -LARGE; 10 MM IN DIAMETER GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL -FIBROSIS, PERIBILIARY,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL MAMMARY, FEMALE (MF) : -HYPERPLASIA, GLANDULAR,-MINIMAL CERVIX (CV) : -HYPERPLASIA, STROMAL,-MODERATELY SEVERE CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 3445 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1687 ANIMAL NUMBER: C93048 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/07/00 STUDY DAY OF DEATH: 719 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 445.2 GRAMS DATE AND TIME OF NECROPSY: 04/07/00 7:52 PROSECTOR: GREGORY CODDINGTON RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), LUNG (LU), PARATHYROID (PT), URINARY BLADDER (UB) 3446 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1688 ANIMAL NUMBER: C93049 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/16/99 STUDY DAY OF DEATH: 484 STUDY WEEK OF DEATH: 70 TERMINAL BODY WEIGHT: 295.5 GRAMS DATE AND TIME OF NECROPSY: 08/16/99 14:20 PROSECTOR: TONY HANFELD RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >EYES; RED DISCHARGE; EYES; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; NO FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; RECUMBENT; LATERAL; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; ATAXIC; MODERATE; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) KIDNEY (KD) : -CYST(S); LEFT, CORTICAL SURFACE: SINGLE CLEAR FLUID FILLED CYST, 2 MM IN DIAMETER PITUITARY (PI) : -MASS(ES); SINGLE MASS, 8 X 8 X 5 MM, RED, DARK RED, TAN AND SOFT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT KIDNEY (KD) : -CYST,-PRESENT SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT -PIGMENT,-MINIMAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATELY SEVERE PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT -HYPERPLASIA, GLANDULAR,-MINIMAL CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 3447 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1689 ANIMAL NUMBER: C93049 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/16/99 STUDY DAY OF DEATH: 484 STUDY WEEK OF DEATH: 70 TERMINAL BODY WEIGHT: 295.5 GRAMS DATE AND TIME OF NECROPSY: 08/16/99 14:20 PROSECTOR: TONY HANFELD RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -DISCHARGE-OCULAR; BILATERAL: DRY AND RED MATERIAL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 3448 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1690 ANIMAL NUMBER: C93050 SEX: FEMALE DATE OF DEATH: 03/02/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 03/02/00 14:25 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 683 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: 440.6 GRAMS PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); VENTRAL-ABDOMINAL-RIGHT; WEEK 76; 50 X 50MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); INGUINAL REGION-RIGHT; WEEK 79; 65 X 60MM; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; NONFORMED FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >EYES; RED DISCHARGE; EYES; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE RED AND DARK RED AREAS, UP TO 3 MM IN DIAMETER; COLLECTED WITH ROUTINE SECTIONS HISTOPATHOLOGY LIVER (LI) : -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-SLIGHT -HYPERPLASIA, BILE DUCT,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, PERIPORTAL,-MODERATE -CYST, BILIARY,-PRESENT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE MINIMAL -MINERALIZATION, TUBULAR,-SLIGHT -HYPERPLASIA, TRANSITIONAL CELL,MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SLIGHT PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,MODERATELY SEVERE ADRENAL, CORTEX (AC) : -HYPERPLASIA, CORTICAL,-MODERATE -PIGMENT,-MINIMAL 3449 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1691 ANIMAL NUMBER: C93050 SEX: FEMALE DATE OF DEATH: 03/02/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 03/02/00 14:25 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 683 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: 440.6 GRAMS PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -CYST(S); MULTIPLE CYSTS, UP TO 2 MM IN DIAMETER, FILLED WITH A DARK RED FLUID MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT INGUINAL REGION: SINGLE MASS, 35 X 45 X 55 MM, FIRM, RED, TAN AND LOBULATED; ON SECTION SOME AREAS CONTAIN A TAN SEMIFLUID; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED -MASS #2; RIGHT INGUINAL REGION, CAUDAL TO MASS 1, EXTENDS INTO PERIANAL REGION: SINGLE MASS, 45 X 55 X 70 MM, FIRM, RED, DARK RED, TAN AND LOBULATED; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED CERVIX (CV) : -LARGE; 11 X 13 X 18 MM, FIRM AND WHITE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY) SEVERE PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -M-CARCINOMA,-NEOPLASM PRESENT -CYST / GALACTOCELE,-PRESENT -B-FIBROADENOMA,-NEOPLASM PRESENT -HYPERPLASIA, GLANDULAR,-MODERATE >NOTE:>MASS #1 IS THE FIBROADENOMA CERVIX (CV) : -HYPERPLASIA, STROMAL,-SEVERE CAUSE OF DEATH (CD) : -NEOPLASM, MAMMARY GLAND,-PRESENT 3450 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1692 ANIMAL NUMBER: C93050 SEX: FEMALE DATE OF DEATH: 03/02/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 03/02/00 14:25 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 683 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: 440.6 GRAMS PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY LN, LUMBAR/ILIAC (LM) : -DIFFUSELY RED; RIGHT: DARK RED (CAP 21) GENERAL COMMENT (GC) -BLOOD FILM TAKEN -DISCHARGE-OCULAR; MATERIAL -ANIMAL THIN : BILATERAL: DRY RED LN, LUMBAR/ILIAC (LM) : -HYPERPLASIA, LYMPHOPLASMACYTIC,MODERATE -HEMORRHAGE,-PRESENT 3451 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1693 ANIMAL NUMBER: C93050 SEX: FEMALE DATE OF DEATH: 03/02/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 03/02/00 14:25 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 683 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: 440.6 GRAMS PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) 3452 COVAH^ THE DEVELOPMENT SERVICES COMPANY Sponsor: 3M St. Paul, Minnesota FINAL REPORT Study Title: 104-Week Dietary Chronic Toxicity and Carcinogenicity Study with Perfluorooctane Sulfonic Acid Potassium Salt (PFOS; T-6295) in Rats Author: Peter J. Thomford, PhD Study Completion Date: January 2, 2002 Testing Facility: Covance Laboratories Inc. 3301 Kinsman Boulevard Madison, Wisconsin 53704-2595 Laboratory Study Identification: Covance 6329-183 Sponsor Study Identification: 3M T-6295 Volume IX of IX Page 3453 of 4068 Covance 6329-183 3M T-6295 VOLUME IX OF IX CONTENTS Page APPENDIX 6 (Continued)........................................................................................... 3455 Individual Anatomic Pathology Data....................................................................... 3456 APPENDIX 7................................................................................................................ 4004 Cell Proliferation Report.......................................................................................... 4005 APPENDIX 8................................................................................................................ 4029 Electron Microscopy Report..................................................................................... 4030 3454 APPENDIX 6 (Continued) Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 3455 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1694 ANIMAL NUMBER: C93051 SEX: FEMALE DATE OF DEATH: 07/21/98 STUDY DAY OF DATE AND TIME OF NECROPSY: 07/21/98 13:14 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 239.6 GRAMS PROSECTOR: STEVE VAN ADESTINE RECORDER: LYNN HORNER PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN S T A rr U S LF ADRENAL (AD0) RT ADRENAL (AD1) BRAIN (BR) LF KIDNEY (KD0) RT KIDNEY (KD1) LIVER (LI) LUNG (LU) LF OVARY (OV0) RT OVARY (OV1) SPLEEN (SP) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) UTERUS (UT) LAST CLINICAL OBSERVATIONS .0353 .0352 2.1584 1.0982 1.1061 7.8763 1.4057 .0840 .0747 .4849 .0101 .0106 1.4870 .0147 % .0147 % .9008 % .4583 % .4616 % 3.2873 % .5867 % .0351 % .0312 % .2024 % .0042 % .0044 % .6206 % OBSERVATIONS NECROPSY .0164 .0163 1.0000 .5088 .5125 3.6491 .6513 .0389 .0346 .2247 .0047 .0049 .6889 WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MINIMAL PANCREAS (PA) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL UTERUS (UT) : >NOT REQUIRED TO BE EXAMINED FOR ANIMAL 3456 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1695 ANIMAL NUMBER: C93051 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 239.6 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 13:14 PROSECTOR: STEVE VAN ADESTINE RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY UTERUS (UT) : -LUMEN FILLED WITH FLUID; BILATERAL: CLEAR FLUID GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV) 3457 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1696 ANIMAL NUMBER: C93052 SEX: FEMALE DATE OF DEATH: 09/01/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 09/01/99 15:07 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 500 STUDY WEEK OF DEATH: 72 TERMINAL BODY WEIGHT: 395.4 GRAMS PROSECTOR: JERALD W. JOHNSON RECORDER: TONE EVERTS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; HEAD TILT; LEFT; VERIFIED AT NECROPSY: NOT APPLICABLE >EYES; RED DISCHARGE; EYE-LEFT; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; COLD TO TOUCH; LIMBS-ALL; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) BONE, FEMUR (FE) : >NOTE:>TRIMMING COMMENT: MISSING AT TRIMMING MARROW, FEMUR (FM) : >NOTE:>TRIMMING COMMENT: MISSING AT TRIMMING PITUITARY (PI) : -MASS(ES); 12 X 9 X 7 MM, DARK RED, BROWN, LOBULATED, AND SOFT HISTOPATHOLOGY BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-MINIMAL KIDNEY (KD) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -MINERALIZATION, PELVIC,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-MINIMAL -ANGIECTASIS,-SLIGHT -PIGMENT,-MINIMAL BONE, FEMUR (FE) : >TISSUE MISSING MARROW, FEMUR (FM) : >TISSUE MISSING PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT 3458 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1697 ANIMAL NUMBER: C93052 SEX: FEMALE DATE OF DEATH: 09/01/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 09/01/99 15:07 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 500 STUDY WEEK OF DEATH: 72 TERMINAL BODY WEIGHT: 395.4 GRAMS PROSECTOR: JERALD W. JOHNSON RECORDER: TONE EVERTS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION GENERAL COMMENT (GC) -BLOOD FILM TAKEN -DISCHARGE-OCULAR; MATERIAL -ANIMAL OBESE : LEFT: DARK RED, DRY MAMMARY, FEMALE (MF) : -HYPERPLASIA, GLANDULAR,-SLIGHT VAGINA (VA) : -LUMEN, EXUDATE,-PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 3459 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1698 ANIMAL NUMBER: C93052 SEX: FEMALE DATE OF DEATH: 09/01/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 09/01/99 15:07 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 500 STUDY WEEK OF DEATH: 72 TERMINAL BODY WEIGHT: 395.4 GRAMS PROSECTOR: JERALD W. JOHNSON RECORDER: TONE EVERTS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), HEMATO NEOPLASIA (HN) (CE), 3460 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1699 ANIMAL NUMBER: C93053 SEX: FEMALE DATE OF DEATH: 07/22/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 07/22/99 14:37 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 459 STUDY WEEK OF DEATH: 66 TERMINAL BODY WEIGHT: 334.9 GRAMS PROSECTOR: LYNN HORNER RECORDER: NANCY DIEDRICH PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); VENTRAL-CERVICAL-LEFT; WEEK 42; 70 X 55MM; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; HEAD TILT; RIGHT; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 10 X 8 X 5 MM, DARK RED, GRAY, AND SOFT HISTOPATHOLOGY BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, PERIPORTAL,-SLIGHT KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : -HYPERPLASIA, CORTICAL,-MINIMAL MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,-SLIGHT THYROID (TY) : -HYPERPLASIA, C-CELL,-SLIGHT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT -B-FIBROADENOMA,-NEOPLASM PRESENT 3461 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1700 ANIMAL NUMBER: C93053 SEX: FEMALE DATE OF DEATH: 07/22/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 07/22/99 14:37 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 459 STUDY WEEK OF DEATH: 66 TERMINAL BODY WEIGHT: 334.9 GRAMS PROSECTOR: LYNN HORNER RECORDER: NANCY DIEDRICH PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS(ES); LEFT AXILLARY REGION: SINGLE MASS, 75 X 60 X 23 MM, RED, TAN, GRAY, VASCULAR, LOBULATED AND SOFT; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (AXILLARY) NORMAL (CAP 20) -CYST(S); ALL REGIONS, DIFFUSE: MULTIPLE, TAN, CREAMY SEMIFLUID FILLED CYSTS, UP TO 3 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN CAUSE OF DEATH (CD) : -NEOPLASM, MAMMARY GLAND,-PRESENT 3462 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1701 ANIMAL NUMBER: C93053 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/22/99 STUDY DAY OF DEATH: 459 STUDY WEEK OF DEATH: 66 TERMINAL BODY WEIGHT: 334.9 GRAMS DATE AND TIME OF NECROPSY: 07/22/99 14:37 PROSECTOR: LYNN HORNER RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN), LN, AXILLARY (AX) 3463 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1702 ANIMAL NUMBER: C93054 SEX: FEMALE DATE OF DEATH: 09/05/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 09/05/99 10:52 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 504 STUDY WEEK OF DEATH: 72 TERMINAL BODY WEIGHT: 336.4 GRAMS PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); INGUINAL REGION-LEFT; WEEK 71; 30 X 20MM; VERIFIED AT NECROPSY: OBSERVED >EYES; RED DISCHARGE; EYES; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; ATAXIC; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; HEAD TILT; RIGHT; VERIFIED AT NECROPSY: NOT APPLICABLE >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 12 X 8 X 7 MM, DARK RED AND FIRM HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-SLIGHT -FIBROSIS, PERIBILIARY,-MINIMAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC / HEMORRHAGIC,MODERATE -PIGMENT,-MINIMAL ADRENAL, MEDULLA (MA) : -UNILATERALLY EXAMINED,-PRESENT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),SLIGHT LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT 3464 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1703 ANIMAL NUMBER: C93054 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/05/99 STUDY DAY OF DEATH: 504 STUDY WEEK OF DEATH: 72 TERMINAL BODY WEIGHT: 336.4 GRAMS DATE AND TIME OF NECROPSY: 09/05/99 10:52 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS(ES); LEFT INGUINAL REGION: SINGLE MASS, 30 X 20 X 15 MM, TAN AND FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED GENERAL COMMENT (GC) -BLOOD FILM TAKEN -DISCHARGE-OCULAR; MATERIAL -ANIMAL THIN : BILATERAL: DRY RED HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL MAMMARY, FEMALE (MF) : -B-FIBROADENOMA,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 3465 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1704 ANIMAL NUMBER: C93054 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/05/99 STUDY DAY OF DEATH: 504 STUDY WEEK OF DEATH: 72 TERMINAL BODY WEIGHT: 336.4 GRAMS DATE AND TIME OF NECROPSY: 09/05/99 10:52 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) (SE), 3466 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1705 ANIMAL NUMBER: C93055 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/04/99 STUDY DAY OF DEATH: 533 STUDY WEEK OF DEATH: 77 TERMINAL BODY WEIGHT: 508.0 GRAMS DATE AND TIME OF NECROPSY: 10/04/99 15:17 PROSECTOR: LAURIE J. SCHULLER RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; RED HAIR COAT; HEADENTIRE; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) ADRENAL, CORTEX (AC) : -LARGE; LEFT: 6 X 5 X 5 MM, RIGHT IS 3 MM IN DIAMETER -RED FOCUS(I)/AREAS(I); LEFT: SINGLE DARK RED FOCUS, 2 MM IN DIAMETER HISTOPATHOLOGY EYE (EY) : -DYSTROPHY, CORNEAL IN EARLY STAGES, CONSISTS PRIMARILY OF FOCAL MINERALIZATION,-MINIMAL BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-SEVERE KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATELY SEVERE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATE THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT 3467 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1706 ANIMAL NUMBER: C93055 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/04/99 STUDY DAY OF DEATH: 533 STUDY WEEK OF DEATH: 77 TERMINAL BODY WEIGHT: 508.0 GRAMS DATE AND TIME OF NECROPSY: 10/04/99 15:17 PROSECTOR: LAURIE J. SCHULLER RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 6 X 4 X 4 MM, RED, DARK RED, GRAY, AND SOFT MAMMARY, FEMALE (MF) : -CYST(S); ALL REGIONS, DIFFUSE: MULTIPLE WHITE CREAMY SEMIFLUID FILLED CYSTS, UP TO 3 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION UTERUS (UT) : -THICKENED WALL; BILATERAL, DIFFUSE -CYST(S); LEFT WITHIN WALL: SINGLE CLEAR FLUID FILLED CYST, 5 X 3 X 3 MM VAGINA (VA) : -SEMIFLUID MATERIAL; WITHIN LUMEN: TAN, CREAMY SEMIFLUID GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -STAINS - SKIN/HAIRCOAT; MATERIAL HEAD: RED DRY PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT UTERUS (UT) : -DILATATION,-MODERATE VAGINA (VA) : -LUMEN, EXUDATE,-PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 3468 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1707 ANIMAL NUMBER: C93055 SEX: FEMALE DATE OF DEATH: 10/04/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 10/04/99 15:17 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 533 STUDY WEEK OF DEATH: 77 TERMINAL BODY WEIGHT: 508.0 GRAMS PROSECTOR: LAURIE J. SCHULLER RECORDER: LAURIE J. SCHULLER PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), HEMATO NEOPLASIA (HN) 3469 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1708 ANIMAL NUMBER: C93056 SEX: FEMALE DATE OF DEATH: 03/10/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 03/10/00 13:04 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 691 STUDY WEEK OF DEATH: 99 TERMINAL BODY WEIGHT: 339.4 GRAMS PROSECTOR: TODD ACKER RECORDER: TONE EVERTS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); INGUINAL REGION-RIGHT; WEEK 95; 25 X 25MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); VENTRAL-ABDOMINAL; WEEK 99; 20 X 20MM; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -MASS(ES); RIGHT MEDIAN LOBE, NEAR HILUS: SINGLE MASS, 7 X 5 X 5 MM, TAN, BROWN, AND SOFT; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (HEPATIC) NOT IDENTIFIED OVARY (OV) : -CYST(S); RIGHT: MULTIPLE CLEAR FLUID FILLED CYSTS, UP TO 2 MM IN DIAMETER HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL -B-CHOLANGIOMA,-NEOPLASM PRESENT KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-MINIMAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATELY SEVERE -PIGMENT,-MINIMAL OVARY (OV) : >UNREMARKABLE 3470 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1709 ANIMAL NUMBER: C93056 SEX: FEMALE DATE OF DEATH: 03/10/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 03/10/00 13:04 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 691 STUDY WEEK OF DEATH: 99 TERMINAL BODY WEIGHT: 339.4 GRAMS PROSECTOR: TODD ACKER RECORDER: TONE EVERTS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY THYMUS (TH) : -MISSING/NOT IDENTIFIED; NOT IDENTIFIED, POSSIBLE REMNANTS COLLECTED PITUITARY (PI) : -MASS(ES); 10 X 9 X 8 MM, DARK RED, RED, TAN, AND SOFT MAMMARY, FEMALE (MF) : -MASS(ES); MID PERIANAL REGION: SINGLE MASS, 30 X 25 X 15 MM, DARK RED, RED, TAN, BROWN, AND SOFT; SAME ON CUT SURFACE; COLLECTED; POSSIBLY CONSISTENT WITH MASS (VENTRAL ABDOMINAL) NOTED IN CLINICAL OBSERVATION; REGIONAL LN (INGUINAL) NOT IDENTIFIED -MASS #2; RIGHT INGUINAL REGION: SINGLE MASS, 35 X 25 X 20 MM, DARK RED, TAN, AND FIRM, CUT SURFACE EXUDES A DARK REDDISH-BROWN SEMIFLUID; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED UTERUS (UT) : -CYST(S); BILATERAL WITHIN WALLS: MULTIPLE CLEAR FLUID FILLED CYSTS, UP TO 2 MM IN DIAMETER THYMUS (TH) : >SECTION EXAMINED; TISSUE NOT PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL MAMMARY, FEMALE (MF) : -M-CARCINOMA,- 2 NEOPLASMS PRESENT -CYST / GALACTOCELE,-PRESENT -B-FIBROADENOMA,-NEOPLASM PRESENT -HYPERPLASIA, GLANDULAR,-MODERATE >NOTE:>FIBROADENOMA IS ON SLIDE #19 MASSES 1 AND 2 ARE CARCINOMAS UTERUS (UT) : -HYPERPLASIA, CYSTIC, ENDOMETRIAL,SLIGHT 3471 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1710 ANIMAL NUMBER: C93056 SEX: FEMALE DATE OF DEATH: 03/10/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 03/10/00 13:04 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 691 STUDY WEEK OF DEATH: 99 TERMINAL BODY WEIGHT: 339.4 GRAMS PROSECTOR: TODD ACKER RECORDER: TONE EVERTS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN CERVIX (CV) : -HYPERPLASIA, MUCINOUS,-SLIGHT VAGINA (VA) : -HYPERPLASIA, MUCINOUS,-SEVERE CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 3472 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1711 ANIMAL NUMBER: C93056 SEX: FEMALE DATE OF DEATH: 03/10/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 03/10/00 13:04 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 691 STUDY WEEK OF DEATH: 99 TERMINAL BODY WEIGHT: 339.4 GRAMS PROSECTOR: TODD ACKER RECORDER: TONE EVERTS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), HEMATO NEOPLASIA (HN) 3473 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1712 ANIMAL NUMBER: C93057 SEX: FEMALE DATE OF DEATH: 03/10/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 03/10/00 11:30 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 691 STUDY WEEK OF DEATH: 99 TERMINAL BODY WEIGHT: 377.9 GRAMS PROSECTOR: TODD ACKER RECORDER: TONE EVERTS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); AXILLARY REGION-RIGHT; WEEK 68; 75 X 60MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); VENTRAL-ABDOMINAL; WEEK 97; 15 X 15MM; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) ADRENAL, CORTEX (AC) : -LARGE; LEFT: 6 X 6 X 3 MM (RIGHT IS 5 X 4 X 2 MM) HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -HEMORRHAGE,-MODERATE -I-CARCINOMA,-NEOPLASM PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL -FIBROSIS, PERIBILIARY,-MINIMAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATELY SEVERE ADRENAL, CORTEX (AC) : -HYPERTROPHY, CORTICAL CELL,-MODERATELY SEVERE -DEGENERATION, CYSTIC / HEMORRHAGIC,MODERATELY SEVERE -PIGMENT,-MINIMAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),SLIGHT 3474 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1713 ANIMAL NUMBER: C93057 SEX: FEMALE DATE OF DEATH: 03/10/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 03/10/00 11:30 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 691 STUDY WEEK OF DEATH: 99 TERMINAL BODY WEIGHT: 377.9 GRAMS PROSECTOR: TODD ACKER RECORDER: TONE EVERTS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 10 X 7 X 5 MM, RED, DARK RED, TAN, AND SOFT MAMMARY, FEMALE (MF) : -MASS(ES); LEFT PERIANAL REGION: SINGLE MASS, 30 X 25 X 20 MM, RED, DARK RED, TAN, AND FIRM; CUT SURFACE EXUDES A DARK RED SEMIFLUID; COLLECTED; POSSIBLY CONSISTENT WITH MASS (VENTRAL ABDOMINAL) NOTED IN CLINICAL OBSERVATION; REGIONAL LN (INGUINAL) NOT IDENTIFIED -MASS #2; RIGHT AXILLARY REGION: SINGLE MASS, 80 X 60 X 50 MM, TAN, RED, DARK RED, AND FIRM; SAME ON CUT SURFACE; PORTION COLLECTED; REGIONAL LN (AXILLARY) NORMAL (CAP 21) UTERUS (UT) : -MASS(ES); WITHIN LUMEN OF RIGHT DISTAL HORN: SINGLE MASS, 12 X 4 X 4 MM, TAN, RED, AND SOFT; COLLECTED INTACT; REGIONAL LN (LUMBAR) NORMAL (CAP 22) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN PITUITARY (PI) : -M-CARCINOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL MAMMARY, FEMALE (MF) : -M-CARCINOMA,-NEOPLASM PRESENT -B-FIBROADENOMA,-NEOPLASM PRESENT -HYPERPLASIA, GLANDULAR,-MINIMAL >NOTE:>MASS #2 IS THE FIBROADENOMA UTERUS (UT) : -B-POLYP, ENDOMETRIAL, STROMAL,NEOPLASM PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 3475 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1714 ANIMAL NUMBER: C93057 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/10/00 STUDY DAY OF DEATH: 691 STUDY WEEK OF DEATH: 99 TERMINAL BODY WEIGHT: 377.9 GRAMS DATE AND TIME OF NECROPSY: 03/10/00 11:30 PROSECTOR: TODD ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -ANIMAL THIN THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN), LN, AXILLARY (AX), LN, LUMBAR/ILIAC (LM) (CE), 3476 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1715 ANIMAL NUMBER: C93058 SEX: FEMALE DATE OF DEATH: 05/15/98 STUDY DAY OF DATE AND TIME OF NECROPSY: 05/15/98 8:38 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 235.6 GRAMS PROSECTOR: JODI THIEREN RECORDER: DEBBIE PIRKEL PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN STATUS LF ADRENAL (AD0) RT ADRENAL (AD1) BRAIN (BR) LF KIDNEY (KD0) RT KIDNEY (KD1) LIVER (LI) LUNG (LU) LF OVARY (OV0) RT OVARY (OV1) SPLEEN (SP) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) UTERUS (UT) ------------------------------------ 7.6075 -------------------------------------------------- ------------------------------------ 3.2293 % -------------------------------------------------- -------------------------------------------------------------------------------------------- NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN WEIGHT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS 3477 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1716 ANIMAL NUMBER: C93058 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 235.6 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 8:38 PROSECTOR: JODI THIEREN RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) 3478 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1717 ANIMAL NUMBER: C93059 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 579.6 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 9:40 PROSECTOR: TROY ELLENBECKER RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-SLIGHT -GRANULAR CYTOPLASM, EOSINOPHILIC, CENTRILOBULAR,-SLIGHT -PIGMENT, HEPATOCELLULAR, CENTRILOBULAR,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MODERATE -HYPERPLASIA, BILE DUCT,-SLIGHT -FIBROSIS, PERIBILIARY,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, PELVIC,-MINIMAL PANCREAS (PA) : -INFILTRATION, FAT, INTERSTITIAL,SLIGHT 3479 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1718 ANIMAL NUMBER: C93059 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 579.6 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 9:40 PROSECTOR: TROY ELLENBECKER RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN THYROID (TY) : -HYPERPLASIA, C-CELL,-SLIGHT MAMMARY, FEMALE (MF) : -HYPERPLASIA, GLANDULAR,-SLIGHT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 3480 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1719 ANIMAL NUMBER: C93059 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 579.6 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 9:40 PROSECTOR: TROY ELLENBECKER RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PARATHYROID (PT), URINARY BLADDER (UB) 3481 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1720 ANIMAL NUMBER: C93060 SEX: FEMALE DATE OF DEATH: 04/21/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/21/00 8:10 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 473.1 GRAMS PROSECTOR: TODD ACKER RECORDER: TONE EVERTS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); AXILLARY REGION-LEFT; WEEK 93; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -LIGHT FOCUS(I)/AREA(S); AT BIFURCATION OF MEDIAN LOBES: SINGLE YELLOW AREA, 4 X 3 MM (CAP 22) ADRENAL, CORTEX (AC) : -LARGE; LEFT: 7 X 5 X 4 MM (RIGHT IS 5 X 4 X 3 MM) HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -NECROSIS, INDIVIDUAL HEPATOCYTE,MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL -LIPIDOSIS, SUBCAPSULAR (TENSION LIPIDOSIS),-MODERATELY SEVERE KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, PELVIC,-MINIMAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT -HYPERTROPHY, CORTICAL CELL,-SLIGHT -HYPERPLASIA, CORTICAL,-MINIMAL -PIGMENT,-MINIMAL ADRENAL, MEDULLA (MA) : -B-PHEOCHROMOCYTOMA,-NEOPLASM PRESENT THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : -HYPERPLASIA,-MODERATE 3482 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1721 ANIMAL NUMBER: C93060 SEX: FEMALE DATE OF DEATH: 04/21/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/21/00 8:10 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 473.1 GRAMS PROSECTOR: TODD ACKER RECORDER: TONE EVERTS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -RED FOCUS(I)/AREA(S); MID LEFT ASPECT: SINGLE DARK RED FOCUS, 2 MM IN DIAMETER MAMMARY, FEMALE (MF) : -MASS(ES); LEFT AXILLARY REGION: SINGLE MASS, 18 X 12 X 7 MM, RED, DARK RED, TAN, AND FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (AXILLARY) NORMAL (CAP 23) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -MALOCCLUSION; BILATERAL, UPPER INCISORS MAMMARY, FEMALE (MF) : -HYPERPLASIA, GLANDULAR,-MINIMAL -B-ADENOMA,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, AXILLARY (AX) : -HYPERPLASIA, LYMPHOPLASMACYTIC,-SLIGHT 3483 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1722 ANIMAL NUMBER: C93060 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 473.1 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 8:10 PROSECTOR: TODD ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), LUNG (LU), PARATHYROID (PT), URINARY BLADDER (UB) 3484 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1723 ANIMAL NUMBER: C93061 SEX: FEMALE DATE OF DEATH: 10/29/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 10/29/99 8:08 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DEATH: 558 STUDY WEEK OF DEATH: 80 TERMINAL BODY WEIGHT: NOT ENTERED PROSECTOR: JARED FISHEL RECORDER: TONE EVERTS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; PALE; ENTIRE BODY; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; THIN; VERIFIED AT NECROPSY: NOT OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; HUNCHED; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) KIDNEY (KD) : -CYST(S); RIGHT, INVOLVING ENTIRE ORGAN: SINGLE CLEAR FLUID FILLED CYST, 40 MM IN DIAMETER -ROUGH SURFACE, DIFFUSE; LEFT -LARGE; LEFT: 28 X 17 X 17 MM (RIGHT SEE CYST COMMENT) ADRENAL, CORTEX (AC) : -LARGE; RIGHT: 7 X 6 X 4 MM (LEFT IS 4 X 3 X 2 MM) HISTOPATHOLOGY BRAIN (BR) : >UNREMARKABLE LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL -VACUOLATION, HEPATOCELLULAR,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SEVERE -DEGENERATION, CYSTIC, UNILATERAL,SEVERE SPLEEN (SP) : -PIGMENT, INCREASED,-MINIMAL PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-MODERATE ADRENAL, CORTEX (AC) : -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL -HYPERTROPHY, CORTICAL CELL,-MODERATE -DEGENERATION, CYSTIC / HEMORRHAGIC,MODERATE 3485 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1724 ANIMAL NUMBER: C93061 SEX: FEMALE DATE OF DEATH: 10/29/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 10/29/99 8:08 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DEATH: 558 STUDY WEEK OF DEATH: 80 TERMINAL BODY WEIGHT: NOT ENTERED PROSECTOR: JARED FISHEL RECORDER: TONE EVERTS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY THYROID (TY) : -LARGE; BILATERAL: EACH IS 7 X 3 X 3 MM -DIFFUSELY LIGHT; BILATERAL: TAN PITUITARY (PI) : -MASS(ES); 8 MM IN DIAMETER, DARK RED, TAN, AND SOFT MARROW, FEMUR (FM) : -HYPERPLASIA, ERYTHROID, MARROW,MODERATELY SEVERE MARROW, STERNUM (SE) : -HYPERPLASIA, ERYTHROID, MARROW,MODERATE THYMUS (TH) : >SECTION EXAMINED; TISSUE NOT PRESENT HEART (HT) : -CARDIOMYOPATHY,-MODERATE THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : -MINERALIZATION, GLANDULAR MUCOSA,SLIGHT JEJUNUM (JE) : >AUTOLYTIC ILEUM (IL) : >AUTOLYTIC CECUM (CE) : >AUTOLYTIC COLON (CO) : >AUTOLYTIC 3486 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1725 ANIMAL NUMBER: C93061 SEX: FEMALE DATE OF DEATH: 10/29/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 10/29/99 8:08 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DEATH: 558 STUDY WEEK OF DEATH: 80 TERMINAL BODY WEIGHT: NOT ENTERED PROSECTOR: JARED FISHEL RECORDER: TONE EVERTS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -THICKENED; LEFT AND RIGHT INGUINAL REGIONS: TAN AND FIRM; COLLECTED ON ROUTINE SECTION UTERUS (UT) : -CYST(S); RIGHT, WITHIN WALL: SINGLE CLEAR FLUID FILLED CYST, 5 MM IN DIAMETER LN, AXILLARY (AX) : -LARGE; LEFT: 8 MM IN DIAMETER (RIGHT IS 3 X 2 X 1 MM) (CAP 24) -DIFFUSELY RED; BILATERAL: DARK RED LN, RENAL (RL) : -LARGE; BILATERAL: LEFT IS 9 X 4 X 4 MM, RIGHT IS 4 X 3 X 3 MM (CAP 23) -DIFFUSELY RED; LEFT: DARK RED (CAP 23) AORTA (AO) : -LARGE; DIFFUSE: UP TO 4 MM IN DIAMETER (CAP 21) -FIRM; DIFFUSE (CAP 21) URETER (UE) : -LARGE; BILATERAL: UP TO 3 MM IN DIAMETER (CAP 22) MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT -B-FIBROADENOMA,-NEOPLASM PRESENT -B-ADENOMA,-NEOPLASM PRESENT >NOTE:>SEE SLIDE #24 UTERUS (UT) : -DILATATION,-MODERATELY SEVERE CAUSE OF DEATH (CD) : -NEPHROPATHY,-PRESENT LN, AXILLARY (AX) : -HEMORRHAGE,-PRESENT -INFILTRATE, MACROPHAGE, MODERATE LN, RENAL (RL) : -HEMORRHAGE,-PRESENT -INFILTRATE, MACROPHAGE, SLIGHT PIGMENTED,PIGMENTED,- AORTA (AO) : -MINERALIZATION,-SLIGHT URETER (UE) : >UNREMARKABLE 3487 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1726 ANIMAL NUMBER: C93061 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 10/29/99 STUDY DAY OF DEATH: 558 STUDY WEEK OF DEATH: 80 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 10/29/99 8:08 PROSECTOR: JARED FISHEL RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -GI TRACT-DARK DRY CONTENT; STOMACH: FILLED WITH A BROWN CLOTH-LIKE MATERIAL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, INGUINAL (IN), LN, OTHER (LN), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), RECTUM SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) (RE), 3488 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1727 ANIMAL NUMBER: C93062 SEX: FEMALE DATE OF DEATH: 04/19/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/19/00 10:51 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 401.4 GRAMS PROSECTOR: DAVID SCHUETTE RECORDER: LAURIE J. SCHULLER PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) OVARY (OV) : -CYST(S); LEFT: SINGLE CLEAR FLUID FILLED CYST, 3 MM IN DIAMETER THYROID (TY) : -LARGE; RIGHT: 5 X 4 X 3 MM (LEFT IS 4 X 2 X 2 MM) HISTOPATHOLOGY BRAIN (BR) : >UNREMARKABLE LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL -INFLAMMATION, SUPPURATIVE, PELVIC,MINIMAL PANCREAS (PA) : -INFILTRATION, FAT, INTERSTITIAL,SLIGHT OVARY (OV) : -CYST,-PRESENT LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-SLIGHT -M-CARCINOMA, FOLLICULAR CELL,-NEOPLASM PRESENT 3489 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1728 ANIMAL NUMBER: C93062 SEX: FEMALE DATE OF DEATH: 04/19/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/19/00 10:51 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 401.4 GRAMS PROSECTOR: DAVID SCHUETTE RECORDER: LAURIE J. SCHULLER PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 8 X 7 X 5 MM, DARK RED, TAN, AND SOFT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -MALOCCLUSION; BILATERAL UPPER INCISORS PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -HYPERPLASIA, GLANDULAR,-MINIMAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 3490 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1729 ANIMAL NUMBER: C93062 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 401.4 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 10:51 PROSECTOR: DAVID SCHUETTE RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PARATHYROID (PT), URINARY BLADDER (UB) 3491 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1730 ANIMAL NUMBER: C93063 SEX: FEMALE DATE OF DEATH: 04/19/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/19/00 13:04 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 482.3 GRAMS PROSECTOR: DAVID SCHUETTE RECORDER: LAURIE J. SCHULLER PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >EYES; RED DISCHARGE; EYE-RIGHT; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); AXILLARY REGION-RIGHT; WEEK 100; 40 X 30MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); INGUINAL REGION-RIGHT; WEEK 101; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); VENTRAL-CERVICAL; WEEK 102; 35 X 35MM; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; AXILLARY REGION-RIGHT; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 8 X 6 X 5 MM, DARK RED, TAN, AND SOFT HISTOPATHOLOGY BRAIN (BR) : -I-CARCINOMA,-FROM PITUITARY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-SLIGHT -GRANULAR CYTOPLASM, EOSINOPHILIC, CENTRILOBULAR,-SLIGHT -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-SLIGHT -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL -HYPERPLASIA, BILE DUCT,-SLIGHT -FIBROSIS, PERIBILIARY,-SLIGHT KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-SLIGHT THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PITUITARY (PI) : -M-CARCINOMA,-NEOPLASM PRESENT 3492 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1731 ANIMAL NUMBER: C93063 SEX: FEMALE DATE OF DEATH: 04/19/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/19/00 13:04 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 482.3 GRAMS PROSECTOR: DAVID SCHUETTE RECORDER: LAURIE J. SCHULLER PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT AXILLARY REGION: SINGLE MASS, 50 X 40 X 30 MM, RED, TAN, AND FIRM; SAME ON CUT SURFACE; SKIN OVERLYING MASS HAS A MOIST DARK BROWN AND RED ULCERATED AREA, 25 X 15 MM; COLLECTED; REGIONAL LN (AXILLARY) NORMAL (CAP 22) -MASS #2; RIGHT CERVICAL REGION: SINGLE MASS, 45 X 30 X 20 MM, TAN, LOBULATED, AND SOFT; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (MANDIBULAR) NORMAL (CAP 24) -MASS #3; RIGHT ABDOMINAL REGION: SINGLE MASS, 15 X 15 X 7 MM, TAN AND FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED UTERUS (UT) : -CYST(S); BILATERAL, WITHIN WALLS: MULTIPLE CLEAR FLUID FILLED CYSTS, UP TO 5 MM IN DIAMETER MAMMARY, FEMALE (MF) : -M-CARCINOMA,-NEOPLASM PRESENT -CYST / GALACTOCELE,-PRESENT -B-FIBROADENOMA,-NEOPLASM PRESENT -HYPERPLASIA, GLANDULAR,-SLIGHT -B-ADENOMA,-NEOPLASM PRESENT >NOTE:>MASS 1 IS A CARCINOMA MASS 2 IS A FIBROADENOMA UTERUS (UT) : -DILATATION,-MODERATE CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, AXILLARY (AX) : -HEMORRHAGE,-PRESENT -HYPERPLASIA, LYMPHOPLASMACYTIC,-SLIGHT LN, MANDIBULAR (MN) : -HYPERPLASIA, LYMPHOPLASMACYTIC,MODERATE 3493 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1732 ANIMAL NUMBER: C93063 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 482.3 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 13:04 PROSECTOR: DAVID SCHUETTE RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -DISCHARGE-OCULAR; RIGHT: DRY DARK RED MATERIAL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUm /s KULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PARATHYROID (PT), URINARY BLADDER (UB) 3494 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1733 ANIMAL NUMBER: C93064 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 242.5 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 11:12 PROSECTOR: LAURIE J. SCHULLER RECORDER: KIMBERLY DURLAND POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) LF ADRENAL (AD0) RT ADRENAL (AD1) BRAIN (BR) LF KIDNEY (KD0) RT KIDNEY (KD1) LIVER (LI) LUNG (LU) LF OVARY (OV0) RT OVARY (OV1) SPLEEN (SP) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) UTERUS (UT) .0300 .0306 2.0301 1.2222 1.1563 9.0107 1.3975 .0856 .0768 .4442 .0098 .0076 .6495 .0124 % .0126 % .8372 % .5040 % .4768 % 3.7158 % .5763 % .0353 % .0317 % .1832 % .0040 % .0031 % .2678 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0148 .0151 1.0000 .6020 .5696 4.4385 .6884 .0422 .0378 .2188 .0048 .0037 .3199 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED KIDNEY (KD) : -LARGE PELVIS(ES); BILATERAL -GRANULAR MATERIAL; BILATERAL: MULTIPLE WHITE PINPOINT GRANULES LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -NECROSIS, INDIVIDUAL HEPATOCYTE,MINIMAL KIDNEY (KD) : -DILATATION, PELVIC,-SLIGHT -MINERALIZATION, PELVIC,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 3495 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1734 ANIMAL NUMBER: C93064 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 242.5 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 11:12 PROSECTOR: LAURIE J. SCHULLER RECORDER: KIMBERLY DURLAND POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV) 3496 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1735 ANIMAL NUMBER: C93065 SEX: FEMALE DATE OF DEATH: 01/24/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 01/24/00 12:35 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 645 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: 338.6 GRAMS PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); AXILLARY REGION-RIGHT; WEEK 83; 15 X 15MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); VENTRAL-ABDOMINAL-RIGHT; WEEK 86; 10 X 10MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); AXILLARY REGION-LEFT; WEEK 87; VERIFIED AT NECROPSY: OBSERVED >RESPIRATION; AUDIBLE; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) ADRENAL, CORTEX (AC) : -RED FOCUS(I)/AREAS(I); LEFT: SINGLE DARK RED AREA, 4 X 3 MM OVARY (OV) : -CYST(S); LEFT: SINGLE CLEAR FLUID FILLED CYST, 5 MM IN DIAMETER HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, PELVIC,-SLIGHT SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE PANCREAS (PA) : -INFILTRATE, LYMPHOHISTIOCYTIC,-SLIGHT -FIBROSIS,-SLIGHT ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MINIMAL -PIGMENT,-SLIGHT OVARY (OV) : -CYST,-PRESENT MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,-MODERATE 3497 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1736 ANIMAL NUMBER: C93065 SEX: FEMALE DATE OF DEATH: 01/24/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 01/24/00 12:35 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 645 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: 338.6 GRAMS PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY LUNG (LU) : -LIGHT FOCUS(I)/AREA(S); ALONG MARGIN OF LEFT LOBE: SINGLE GREEN AREA, 5 X 4 MM PITUITARY (PI) : -MASS(ES); 15 X 7 X 7 MM, DARK RED, TAN AND FIRM MAMMARY, FEMALE (MF) : -CYST(S); ALL REGIONS, DIFFUSE: MULTIPLE TAN CREAMY FLUID FILLED CYSTS, UP TO 20 X 15 X 4 MM; COLLECTED ON ROUTINE SECTION; POSSIBLY CONSISTENT WITH ALL MASSES NOTED IN CLINICAL OBSERVATIONS THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),SLIGHT -HYPERPLASIA, EPITHELIAL,-MODERATE LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,MODERATE -INFLAMMATION, GRANULOMATOUS,-SLIGHT -HYPERPLASIA, TYPE II PNEUMOCYTE,SLIGHT >NOTE:>SOME GRANULOMAS CONTAIN FOREIGN MATERIAL HEART (HT) : -CARDIOMYOPATHY,-MINIMAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT -HYPERPLASIA, GLANDULAR,-MODERATE VAGINA (VA) : -LUMEN, EXUDATE,-PRESENT 3498 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1737 ANIMAL NUMBER: C93065 SEX: FEMALE DATE OF DEATH: 01/24/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 01/24/00 12:35 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 645 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: 338.6 GRAMS PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) -BLOOD FILM TAKEN CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), HEMATO NEOPLASIA (HN) 3499 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1738 ANIMAL NUMBER: C93066 SEX: FEMALE DATE OF DEATH: 01/24/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 01/24/00 13:03 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 645 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: 520.0 GRAMS PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK 54; 110 X 110MM MASSES ON LEFT SIDE ARE MEASURED AS ONE MASS; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); VENTRAL-ABDOMINAL-RIGHT; WEEK 70; 35 X 35MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); AXILLARY REGION-LEFT; WEEK 84; 30 X 20MM; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; VENTRALABDOMINAL-RIGHT; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -DIFFUSELY LIGHT; ALL LOBES: TAN SPLEEN (SP) : -LARGE; 51 X 12 X 10 MM HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-SLIGHT -HYPERPLASIA, BILE DUCT,-MINIMAL -FIBROSIS, PERIBILIARY,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, TUBULAR,-MINIMAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SEVERE ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT -HYPERPLASIA, CORTICAL,-MINIMAL -PIGMENT,-MINIMAL MARROW, FEMUR (FM) : -HYPERPLASIA, ERYTHROID, MARROW MODERATE MARROW, STERNUM (SE) : -HYPERPLASIA, ERYTHROID, MARROW MODERATE 3500 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1739 ANIMAL NUMBER: C93066 SEX: FEMALE DATE OF DEATH: 01/24/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 01/24/00 13:03 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 645 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: 520.0 GRAMS PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -LARGE; 7 X 7 X 3 MM, APPEARS SWOLLEN -RED FOCUS(I)/AREA(S); ALL REGIONS, DIFFUSE: MULTIPLE RED AREAS, UP TO 3 X 2 MM MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT INGUINAL REGION: SINGLE MASS, 40 X 30 X 25 MM, TAN, RED, GREEN, VASCULAR AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; OVERLYING SKIN HAS MULTIPLE RED MOIST ULCERATED AREAS, UP TO 11 MM IN DIAMETER; COLLECTED INTACT WITH MASS; REGIONAL LN (SEE INGUINAL); POSSIBLY CONSISTENT WITH MASS (VENTRAL ABDOMINAL RIGHT) NOTED IN CLINICAL OBSERVATIONS -MASS #2; LEFT INGUINAL REGION: MULTIPLE MASSES, UP TO 70 X 50 X 39 MM, ALL ARE TAN, VASCULAR AND FIRM; SAME ON CUT SURFACES; ALL COLLECTED INTACT; REGIONAL LN (SEE INGUINAL); POSSIBLY CONSISTENT WITH MASS (VENTRAL ABDOMINAL LEFT) NOTED IN CLINICAL OBSERVATIONS THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),SLIGHT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -M-CARCINOMA,-NEOPLASM PRESENT -CYST / GALACTOCELE,-PRESENT -INFLAMMATION, CHRONIC-ACTIVE,-SEVERE -B-FIBROADENOMA,-NEOPLASM PRESENT >NOTE:>MASS #1 IS THE CARCINOMA MASS #3 IS AN INFLAMED GALACTOCELE 3501 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1740 ANIMAL NUMBER: C93066 SEX: FEMALE DATE OF DEATH: 01/24/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 01/24/00 13:03 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 645 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: 520.0 GRAMS PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS #3; LEFT AXILLARY REGION: SINGLE MASS, 25 X 15 X 15 MM, RED, TAN, VASCULAR AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; OVERLYING SKIN HAS A SINGLE MOIST RED ULCERATED AREA, 3 MM IN DIAMETER; COLLECTED INTACT WITH MASS; REGIONAL LN (SEE AXILLARY) LN, AXILLARY (AX) : -LARGE; BILATERAL: EACH IS 10 X 3 X 2 MM (CAP 22) LN, INGUINAL (IN) : -LARGE; RIGHT: 10 X 5 X 2 MM (LEFT NOT IDENTIFIED) (CAP 21) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN CAUSE OF DEATH (CD) : -NEOPLASM, MAMMARY GLAND,-PRESENT LN, AXILLARY (AX) : -HYPERPLASIA, LYMPHOPLASMACYTIC,MODERATELY SEVERE LN, INGUINAL (IN) : -HYPERPLASIA, LYMPHOPLASMACYTIC,MODERATELY SEVERE 3502 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1741 ANIMAL NUMBER: C93066 SEX: FEMALE DATE OF DEATH: 01/24/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 01/24/00 13:03 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 645 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: 520.0 GRAMS PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 3503 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1742 ANIMAL NUMBER: C93067 SEX: FEMALE DATE OF DEATH: 05/15/98 STUDY DAY OF DATE AND TIME OF NECROPSY: 05/15/98 8:45 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 207.5 GRAMS PROSECTOR: LYNN HORNER RECORDER: DEBBIE PIRKEL PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN STATUS LF ADRENAL (AD0) RT ADRENAL (AD1) BRAIN (BR) LF KIDNEY (KD0) RT KIDNEY (KD1) LIVER (LI) LUNG (LU) LF OVARY (OV0) RT OVARY (OV1) SPLEEN (SP) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) UTERUS (UT) ------------------------------------ 8.1068 -------------------------------------------------- ------------------------------------ 3.9061 % -------------------------------------------------- -------------------------------------------------------------------------------------------- NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN WEIGHT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS 3504 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1743 ANIMAL NUMBER: C93067 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 207.5 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 8:45 PROSECTOR: LYNN HORNER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) 3505 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1744 ANIMAL NUMBER: C93068 SEX: FEMALE DATE OF DEATH: 04/06/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/06/00 10:22 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DEATH: 718 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: NOT ENTERED PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; ALOPECIA; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; HEAD TILT; RIGHT; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; THIN; VERIFIED AT NECROPSY: NOT OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) ADRENAL, CORTEX (AC) : -LARGE; LEFT: 9 X 5 X 5 MM (RIGHT IS 5 MM IN DIAMETER) HISTOPATHOLOGY BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL -FIBROSIS, PERIBILIARY,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, PELVIC,-SLIGHT -INFLAMMATION, SUPPURATIVE, PELVIC,MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-MODERATE ADRENAL, CORTEX (AC) : -HYPERTROPHY, CORTICAL CELL,-MODERATELY SEVERE -DEGENERATION, CYSTIC / HEMORRHAGIC,MODERATELY SEVERE -PIGMENT,-SLIGHT 3506 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1745 ANIMAL NUMBER: C93068 SEX: FEMALE DATE OF DEATH: 04/06/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/06/00 10:22 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DEATH: 718 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: NOT ENTERED PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 11 X 10 X 8 MM, DARK RED, GREY, LOBULATED AND SOFT SKIN (SK) : -ALOPECIA-DIFFUSE; ENTIRE VENTRAL SURFACE; COLLECTED ON ROUTINE SECTION THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATE LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL MUSCLE, SKELETAL (SM) : -ATROPHY,-MINIMAL NERVE, SCIATIC (SN) : -DEGENERATION,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL ILEUM (IL) : >AUTOLYTIC SKIN (SK) : >UNREMARKABLE MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT -HYPERPLASIA, GLANDULAR,-SLIGHT CERVIX (CV) : -HYPERPLASIA, MUCINOUS,-SLIGHT VAGINA (VA) : -LUMEN, EXUDATE,-PRESENT 3507 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1746 ANIMAL NUMBER: C93068 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 04/06/00 STUDY DAY OF DEATH: 718 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 04/06/00 10:22 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V jA T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -MALOCCLUSION ; BILATERAL, UPPER INCISORS VAGINA (VA) : -HYPERPLASIA, MUCINOUS,-MODERATELY SEVERE CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 3508 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1747 ANIMAL NUMBER: C93068 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 04/06/00 STUDY DAY OF DEATH: 718 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 04/06/00 10:22 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), TRACHEA (TR), ESOPHAGUS (ES), THYROID PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), HEMATO NEOPLASIA (HN) (TY), 3509 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1748 ANIMAL NUMBER: C93069 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/21/00 STUDY DAY OF DEATH: 702 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: 698.6 GRAMS DATE AND TIME OF NECROPSY: 03/21/00 14:51 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); AXILLARY REGION-RIGHT; WEEK 86; 45 X 30MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); AXILLARY REGION-LEFT; WEEK 87; 60 X 50MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); VENTRAL-THORACIC-RIGHT; WEEK 92; 40 X 35MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); INGUINAL REGION-LEFT; WEEK 93; 50 X 50MM; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -LARGE; ALL LOBES: MARGINS ROUNDED -MOTTLED; ALL LOBES: BROWN AND TAN ADRENAL, CORTEX (AC) : -LARGE; BILATERAL: LEFT IS 10 X 7 X 5 MM, RIGHT IS 7 X 5 X 5 MM -MOTTLED; BILATERAL: RED, BROWN AND TAN HISTOPATHOLOGY BRAIN (BR) : >UNREMARKABLE LIVER (LI) : -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL -VACUOLATION, HEPATOCELLULAR,-SEVERE KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL -MINERALIZATION, TUBULAR,-MINIMAL -HYPERPLASIA, TRANSITIONAL CELL,-SLIGHT -THROMBUS,-PRESENT SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL -HYPERTROPHY, CORTICAL CELL,-SEVERE -DEGENERATION, CYSTIC / HEMORRHAGIC,SEVERE ADRENAL, MEDULLA (MA) : -B-PHEOCHROMOCYTOMA,-NEOPLASM PRESENT THYMUS (TH) : >SECTION EXAMINED; TISSUE NOT PRESENT LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL 3510 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1749 ANIMAL NUMBER: C93069 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/21/00 STUDY DAY OF DEATH: 702 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: 698.6 GRAMS DATE AND TIME OF NECROPSY: 03/21/00 14:51 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 8 X 5 X 3 MM; DARK RED, TAN AND SOFT MAMMARY, FEMALE (MF) : -MASS(ES); LEFT AXILLARY REGION: SINGLE MASS, 60 X 45 X 15 MM, RED, DARK RED, TAN AND FIRM; SAME ON CUT SURFACE; SOME SECTIONS EXUDES A TAN CREAMY SEMIFLUID (CAP 22); REGIONAL LN (RIGHT AXILLARY) NORMAL; LEFT NOT IDENTIFIED (CAP 21) -MASS #2; RIGHT AXILLARY REGION: SINGLE MASS, 65 X 39 X 15 MM, RED, DARK RED, TAN, FIRM AND LOBULATED; SAME ON CUT SURFACE (CAP 23); REGIONAL LN (RIGHT AXILLARY) NORMAL; LEFT NOT IDENTIFIED (CAP 21); CONSISTENT WITH MASSES (AXILLARY REGION RIGHT AND VENTRAL THORACIC RIGHT) NOTED IN CLINICAL OBSERVATIONS HEART (HT) : -CARDIOMYOPATHY,-SLIGHT THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT -B-FIBROADENOMA,- 3 NEOPLASMS PRESENT 3511 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1750 ANIMAL NUMBER: C93069 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/21/00 STUDY DAY OF DEATH: 702 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: 698.6 GRAMS DATE AND TIME OF NECROPSY: 03/21/00 14:51 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS #3; LEFT INGUINAL REGION: SINGLE MASS, 55 X 45 X 30 MM, RED, DARK RED, TAN, FIRM AND LOBULATED; SAME ON CUT SURFACE (CAP 24); REGIONAL LN (INGUINAL) NOT IDENTIFIED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT LN, AXILLARY (AX) : -HYPERPLASIA, LYMPHOPLASMACYTIC,MODERATE 3512 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1751 ANIMAL NUMBER: C93069 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/21/00 STUDY DAY OF DEATH: 702 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: 698.6 GRAMS DATE AND TIME OF NECROPSY: 03/21/00 14:51 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) (SE), 3513 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1752 ANIMAL NUMBER: C93070 SEX: FEMALE DATE OF DEATH: 05/15/98 STUDY DAY OF DATE AND TIME OF NECROPSY: 05/15/98 9:21 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 212.8 GRAMS PROSECTOR: JODI THIEREN RECORDER: DEBBIE PIRKEL PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN STATUS LF ADRENAL (AD0) RT ADRENAL (AD1) BRAIN (BR) LF KIDNEY (KD0) RT KIDNEY (KD1) LIVER (LI) LUNG (LU) LF OVARY (OV0) RT OVARY (OV1) SPLEEN (SP) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) UTERUS (UT) ------------------------------------ 8.9068 -------------------------------------------------- ------------------------------------ 4.1861 % -------------------------------------------------- -------------------------------------------------------------------------------------------- NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN WEIGHT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS 3514 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1753 ANIMAL NUMBER: C93070 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 212.8 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 9:21 PROSECTOR: JODI THIEREN RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) 3515 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1754 ANIMAL NUMBER: C93071 SEX: FEMALE DATE OF DEATH: 07/21/98 STUDY DAY OF DATE AND TIME OF NECROPSY: 07/21/98 13:05 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 223.6 GRAMS PROSECTOR: LAURIE J. SCHULLER RECORDER: LYNN HORNER PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) LF ADRENAL (AD0) RT ADRENAL (AD1) BRAIN (BR) LF KIDNEY (KD0) RT KIDNEY (KD1) LIVER (LI) LUNG (LU) LF OVARY (OV0) RT OVARY (OV1) SPLEEN (SP) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) UTERUS (UT) .0328 .0269 1.9629 .9138 .9168 7.5052 1.2561 .0551 .0550 .4538 .0091 .0094 1.0898 .0147 % .0120 % .8779 % .4087 % .4100 % 3.3565 % .5618 % .0246 % .0246 % .2030 % .0041 % .0042 % .4874 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0167 .0137 1.0000 .4655 .4671 3.8235 .6399 .0281 .0280 .2312 .0046 .0048 .5552 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED ADRENAL, CORTEX (AC) : >NOTE:>EMBEDDING COMMENT: ONE ADRENAL MISSING AT EMBEDDING ADRENAL, MEDULLA (MA) : >NOTE:>EMBEDDING COMMENT: ONE ADRENAL MISSING AT EMBEDDING LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MINIMAL PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-MINIMAL ADRENAL, CORTEX (AC) : -UNILATERALLY EXAMINED,-PRESENT ADRENAL, MEDULLA (MA) : -UNILATERALLY EXAMINED,-PRESENT 3516 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1755 ANIMAL NUMBER: C93071 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 223.6 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 13:05 PROSECTOR: LAURIE J. SCHULLER RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY UTERUS (UT) : -LUMEN FILLED WITH FLUID; BILATERAL: CLEAR FLUID GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN UTERUS (UT) : >NOT REQUIRED TO BE EXAMINED FOR ANIMAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), LN, MESENTERIC (MS), OVARY (OV) 3517 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1756 ANIMAL NUMBER: C93072 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 483.6 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 8:27 PROSECTOR: CHANESTA ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); AXILLARY REGION-RIGHT; WEEK 87; 45 X 40MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); INGUINAL REGION-LEFT; WEEK 103; 20 X 20MM; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -LIGHT FOCUS(I)/AREA(S); MARGIN OF RIGHT MEDIAN LOBE: SINGLE TAN FOCUS, 3 X 5 MM; COLLECTED ON ROUTINE SECTION HISTOPATHOLOGY BRAIN (BR) : -MINERALIZATION,-MINIMAL LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -GRANULAR CYTOPLASM, EOSINOPHILIC, CENTRILOBULAR,-MINIMAL -NECROSIS, INDIVIDUAL HEPATOCYTE,MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MODERATE -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, PERIPORTAL,-MINIMAL -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL -ANGIECTASIS,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, PELVIC,-MINIMAL PANCREAS (PA) : -INFILTRATION, FAT, INTERSTITIAL,MINIMAL 3518 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1757 ANIMAL NUMBER: C93072 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 483.6 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 8:27 PROSECTOR: CHANESTA ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY ADRENAL, CORTEX (AC) : -LARGE; RIGHT: 7 X 5 X 4 MM (LEFT IS 5 X 3 X 2 MM) PITUITARY (PI) : -MASS(ES); 7 X 5 X 3 MM, DARK RED, TAN, AND SOFT MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT AXILLARY REGION: SINGLE MASS, 53 X 45 X 26, RED, TAN, FIRM, AND LOBULATED; SOME SECTIONS EXUDE A GREEN CREAMY SEMIFLUID; COLLECTED; REGIONAL LN (AXILLARY) NORMAL (CAP 23) -THICKENED; ALL REGIONS DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION -MASS #2; LEFT INGUINAL REGION: SINGLE MASS, 36 X 30 X 15 MM, RED, TAN, AND SOFT; SAME ON CUT SURFACE; REGIONAL LN (INGUINAL) NOT IDENTIFIED ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC / HEMORRHAGIC,MINIMAL -PIGMENT,-MINIMAL LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -B-FIBROADENOMA,- 2 NEOPLASMS PRESENT -HYPERPLASIA, GLANDULAR,-SLIGHT UTERUS (UT) : -HYPERPLASIA, CYSTIC, ENDOMETRIAL,SLIGHT 3519 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1758 ANIMAL NUMBER: C93072 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 483.6 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 8:27 PROSECTOR: CHANESTA ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY UTERUS (UT) : -CYST(S); RIGHT HORN, WITHIN WALL: SINGLE CLEAR FLUID FILLED CYST, 5 X 4 X 3 MM LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL: (CAP 22) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN DARK RED CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MANDIBULAR (MN) : -HEMORRHAGE,-PRESENT 3520 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1759 ANIMAL NUMBER: C93072 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 483.6 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 8:27 PROSECTOR: CHANESTA ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: ADRENAL, MEDULLA (MA), PARATHYROID (PT), URINARY BLADDER (UB), LN, AXILLARY (AX) 3521 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1760 ANIMAL NUMBER: C93073 SEX: FEMALE DATE OF DEATH: 09/23/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 09/23/99 13:53 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DEATH: 522 STUDY WEEK OF DEATH: 75 TERMINAL BODY WEIGHT: NOT ENTERED PROSECTOR: MICHELE MARGGI RECORDER: JARED FISHEL PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; HEAD TILT; RIGHT; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; HUNCHED; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >RESPIRATION; AUDIBLE; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY HISTOPATHOLOGY BRAIN (BR) : -M-ASTROCYTOMA,-NEOPLASM PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -NECROSIS, INDIVIDUAL HEPATOCYTE,MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, TUBULAR,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : -CONGESTION,-PRESENT -HYPERTROPHY, CORTICAL CELL,-MINIMAL -PIGMENT,-MINIMAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MINIMAL LUNG (LU) : -CONGESTION,-PRESENT -MINERALIZATION, VASCULAR,-MINIMAL 3522 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1761 ANIMAL NUMBER: C93073 SEX: FEMALE DATE OF DEATH: 09/23/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 09/23/99 13:53 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DEATH: 522 STUDY WEEK OF DEATH: 75 TERMINAL BODY WEIGHT: NOT ENTERED PROSECTOR: MICHELE MARGGI RECORDER: JARED FISHEL PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY UTERUS (UT) : -MASS(ES); WITHIN LUMEN OF LEFT HORN: SINGLE MASS, 10 X 5 X 5 MM, RED, DARK RED AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (LUMBAR) NORMAL (CAP 21) GENERAL COMMENT (GC) : -ANIMAL THIN UTERUS (UT) : -B-POLYP, ENDOMETRIAL, STROMAL,NEOPLASM PRESENT VAGINA (VA) : -LUMEN, EXUDATE,-PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, BRAIN,-PRESENT HEAD, CORONAL (HC) : -INFLAMMATION, ACUTE, EAR,-MINIMAL 3523 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1762 ANIMAL NUMBER: C93073 SEX: FEMALE DATE OF DEATH: 09/23/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 09/23/99 13:53 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DEATH: 522 STUDY WEEK OF DEATH: 75 TERMINAL BODY WEIGHT: NOT ENTERED PROSECTOR: MICHELE MARGGI RECORDER: JARED FISHEL PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), HEMATO NEOPLASIA (HN), LN, LUMBAR/ILIAC (LM) 3524 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1763 ANIMAL NUMBER: C93074 SEX: FEMALE DATE OF DEATH: 11/15/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 11/15/99 13:46 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 575 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: 423.7 GRAMS PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; HEAD TILT; RIGHT; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; ATAXIC; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) ADRENAL, CORTEX (AC) : -LARGE; RIGHT: 6 X 5 X 5 MM (LEFT IS 4 X 2 X 2 MM) HISTOPATHOLOGY BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-MODERATE -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-SLIGHT KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC / HEMORRHAGIC,MODERATE -PIGMENT,-SLIGHT -B-ADENOMA,-NEOPLASM PRESENT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATELY SEVERE 3525 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1764 ANIMAL NUMBER: C93074 SEX: FEMALE DATE OF DEATH: 11/15/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 11/15/99 13:46 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 575 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: 423.7 GRAMS PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 15 X 7 X 5 MM, RED, DARK RED, TAN AND FIRM -RED FOCUS(I)/AREA(S); ALL REGIONS, DIFFUSE: MULTIPLE DARK RED AREAS, UP TO 1 MM IN DIAMETER STOMACH, NONGL (SU) : -LIGHT FOCUS(I)/AREA(S); MUCOSA: MULTIPLE TAN SLIGHTLY RAISED AREAS, UP TO 3 X 2 MM VAGINA (VA) : -SEMIFLUID MATERIAL; WITHIN LUMEN: LIGHT GREEN MUCOIDAL MATERIAL GENERAL COMMENT (GC) : -BLOOD FILM TAKEN LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : -EDEMA,-MODERATE -INFLAMMATION, ACUTE,-SLIGHT STOMACH, NONGL (SU) : -ACANTHOSIS / HYPERKERATOSIS,-MODERATE -EDEMA,-PRESENT -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE MAMMARY, FEMALE (MF) : -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL VAGINA (VA) : -INFLAMMATION, CHRONIC,-SLIGHT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 3526 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1765 ANIMAL NUMBER: C93074 SEX: FEMALE DATE OF DEATH: 11/15/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 11/15/99 13:46 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 575 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: 423.7 GRAMS PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), HEMATO NEOPLASIA (HN) 3527 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1766 ANIMAL NUMBER: C93075 SEX: FEMALE DATE OF DEATH: 05/15/98 STUDY DAY OF DATE AND TIME OF NECROPSY: 05/15/98 10:46 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 189.8 GRAMS PROSECTOR: LYNN HORNER RECORDER: DEBBIE PIRKEL PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN STATUS LF ADRENAL (AD0) RT ADRENAL (AD1) BRAIN (BR) LF KIDNEY (KD0) RT KIDNEY (KD1) LIVER (LI) LUNG (LU) LF OVARY (OV0) RT OVARY (OV1) SPLEEN (SP) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) UTERUS (UT) ------------------------------------ 6.6760 -------------------------------------------------- ------------------------------------ 3.5171 % -------------------------------------------------- -------------------------------------------------------------------------------------------- NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN WEIGHT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS 3528 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1767 ANIMAL NUMBER: C93075 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 189.8 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 10:46 PROSECTOR: LYNN HORNER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) 3529 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1768 ANIMAL NUMBER: C93076 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/01/00 STUDY DAY OF DEATH: 653 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: 566.8 GRAMS DATE AND TIME OF NECROPSY: 02/01/00 13:33 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: NOT OBSERVED >APPEARANCE; MISSING; TEETH; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); LIMB-HIND-LEFT; WEEK 59; 120 X 125MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); AXILLARY REGION-RIGHT; WEEK 84; 50 X 50MM; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE DARK RED AREAS, UP TO 3 X 2 MM; SOME ARE SUNKEN WITH A STELLATE PATTERN; COLLECTED ON ROUTINE SECTIONS HISTOPATHOLOGY BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-MINIMAL -NECROSIS, COAGULATIVE,-MINIMAL -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-MODERATE SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SLIGHT ADRENAL, CORTEX (AC) : -PIGMENT,-MINIMAL LN, MESENTERIC (MS) : -HYPERPLASIA, LYMPHOPLASMACYTIC,-SLIGHT MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,MODERATELY SEVERE 3530 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1769 ANIMAL NUMBER: C93076 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/01/00 STUDY DAY OF DEATH: 653 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: 566.8 GRAMS DATE AND TIME OF NECROPSY: 02/01/00 13:33 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 10 X 6 X 6 MM, RED, DARK RED, TAN AND FIRM MAMMARY, FEMALE (MF) : -MASS(ES); LEFT INGUINAL REGION: SINGLE MASS, 130 X 100 X 40 MM, TAN, LOBULATED AND SOFT; ON SECTION EXUDES A TAN CREAMY SEMIFLUID; COLLECTED INTACT; REGIONAL LN (AXILLARY) NORMAL (CAP 21) -MASS #2; RIGHT AXILLARY REGION: SINGLE MASS, 70 X 50 X 30 MM, TAN, RED, LOBULATED, VASCULAR AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (AXILLARY) NORMAL (CAP 21) MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,MODERATELY SEVERE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),SLIGHT LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT SKIN (SK) : -B-LIPOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -M-CARCINOMA,-NEOPLASM PRESENT >NOTE:>FOR DIAGNOSIS OF MASS #1, SKIN SEE 3531 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1770 ANIMAL NUMBER: C93076 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/01/00 STUDY DAY OF DEATH: 653 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: 566.8 GRAMS DATE AND TIME OF NECROPSY: 02/01/00 13:33 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -TEETH MISSING/BROKEN/WORN; BILATERAL LOWER INCISORS -ANIMAL THIN MISSING, UTERUS (UT) : -HYPERPLASIA, CYSTIC, ENDOMETRIAL,SLIGHT VAGINA (VA) : -HYPERPLASIA, MUCINOUS,-MODERATE CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT LN, AXILLARY (AX) : -HYPERPLASIA, LYMPHOPLASMACYTIC,MODERATE 3532 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1771 ANIMAL NUMBER: C93076 SEX: FEMALE DATE OF DEATH: 02/01/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 02/01/00 13:33 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 653 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: 566.8 GRAMS PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), OVARY (OV), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), CERVIX (CV), HEMATO NEOPLASIA (HN) 3533 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1772 ANIMAL NUMBER: C93077 SEX: FEMALE DATE OF DEATH: 04/21/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/21/00 10:21 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 581.9 GRAMS PROSECTOR: JAMES T. CALLEN RECORDER: LAURIE J. SCHULLER PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); TAIL-PROXIMAL; WEEK 97; 25 X 15MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); AXILLARY REGION-LEFT; WEEK 101; 30 X 30MM; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; TAILPROXIMAL; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -MOTTLED; ALL LOBES: RED AND TAN -ROUGH SURFACE, DIFFUSE; ALL LOBES SPLEEN (SP) : -LARGE; 57 X 16 X 11 MM HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -GRANULAR CYTOPLASM, EOSINOPHILIC, CENTRILOBULAR,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-MODERATE -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL -FIBROSIS, PERIBILIARY,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, PERIPORTAL,-MODERATE KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, PELVIC,-MODERATE SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SLIGHT -HYPERPLASIA, LYMPHORETICULAR,-SEVERE PANCREAS (PA) : -INFILTRATION, FAT, INTERSTITIAL,SLIGHT 3534 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1773 ANIMAL NUMBER: C93077 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 581.9 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 10:21 PROSECTOR: JAMES T. CALLEN RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY ADRENAL, CORTEX (AC) : -LARGE; LEFT: 12 X 10 X 9 MM (RIGHT IS 5 X 3 X 3 MM) OVARY (OV) : -CYST(S); RIGHT: SINGLE CLEAR FLUID FILLED CYST, 3 MM IN DIAMETER THYROID (TY) : -LARGE; RIGHT: 6 X 5 X 4 MM (LEFT IS 5 X 2 X 2 MM) PITUITARY (PI) : -MASS(ES); 8 X 6 X 4 MM, DARK RED, TAN, AND SOFT MAMMARY, FEMALE (MF) : -MASS(ES); LEFT AXILLARY REGION: SINGLE MASS, 39 X 27 X 14 MM, TAN, RED, AND SOFT; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (AXILLARY) NORMAL (CAP 22) UTERUS (UT) : -CYST(S); WITHIN WALLS OF LEFT HORN: MULTIPLE CLEAR FLUID FILLED CYSTS, UP TO 3 MM IN DIAMETER ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC / HEMORRHAGIC,SEVERE -PIGMENT,-SLIGHT -B-ADENOMA,-NEOPLASM PRESENT ADRENAL, MEDULLA (MA) : -UNILATERALLY EXAMINED,-PRESENT OVARY (OV) : -CYST,-PRESENT LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL THYROID (TY) : -B-ADENOMA, FOLLICULAR CELL,-NEOPLASM PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -B-FIBROADENOMA,-NEOPLASM PRESENT -HYPERPLASIA, GLANDULAR,-SLIGHT UTERUS (UT) : -HYPERPLASIA, CYSTIC, ENDOMETRIAL,MODERATE 3535 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1774 ANIMAL NUMBER: C93077 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 581.9 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 10:21 PROSECTOR: JAMES T. CALLEN RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY CERVIX (CV) : -CYST(S); WITHIN LUMEN: SINGLE RED FLUID FILLED CYST, 5 MM IN DIAMETER TAIL (TI) : -MASS(ES); 25 MM FROM BASE: 15 MM IN DIAMETER, RED, TAN, AND SOFT; ON SECTION EXUDES A DARK RED FLUID; COLLECTED; REGIONAL LN (SEE LUMBAR); OVERLYING SKIN HAS A DARK RED MOIST ABRADED AREA, 5 MM IN DIAMETER; COLLECTED INTACT WITH MASS LN, LUMBAR/ILIAC (LM) : -MOTTLED; BILATERAL: RED AND TAN (CAP 25) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN CERVIX (CV) : -HYPERPLASIA, CYSTIC,-MODERATE CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, AXILLARY (AX) : -HYPERPLASIA, LYMPHOPLASMACYTIC,MODERATELY SEVERE -INFILTRATE, MACROPHAGE, PIGMENTED,SLIGHT TAIL (TI) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE -M-LIPOSARCOMA,-NEOPLASM PRESENT LN, LUMBAR/ILIAC (LM) : -HYPERPLASIA, LYMPHOPLASMACYTIC,MODERATELY SEVERE -INFILTRATE, MACROPHAGE, PIGMENTED,SLIGHT 3536 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1775 ANIMAL NUMBER: C93077 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 581.9 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 10:21 PROSECTOR: JAMES T. CALLEN RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PARATHYROID (PT), URINARY BLADDER (UB) 3537 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1776 ANIMAL NUMBER: C93078 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 597.4 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 12:58 PROSECTOR: JERALD W. JOHNSON RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); VENTRAL-ABDOMINAL-RIGHT; WEEK 89; 70 X 65MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK 94; 40 X 40MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); AXILLARY REGION-LEFT; WEEK 104; VERIFIED AT NECROPSY: NOT OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) KIDNEY (KD) : -DIFFUSELY DARK; BILATERAL: DARK BROWN ADRENAL, CORTEX (AC) : -DIFFUSELY RED; BILATERAL: DARK RED OVARY (OV) : -CYST(S); BILATERAL: SINGLE CLEAR FLUID FILLED CYST ON EACH, LEFT IS 3 MM IN DIAMETER, RIGHT IS 2 MM IN DIAMETER HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, PELVIC,-MINIMAL PANCREAS (PA) : -INFILTRATION, FAT, INTERSTITIAL,MINIMAL ADRENAL, CORTEX (AC) : -HYPERTROPHY, CORTICAL CELL,-SEVERE -DEGENERATION, CYSTIC / HEMORRHAGIC,SEVERE ADRENAL, MEDULLA (MA) : -UNILATERALLY EXAMINED,-PRESENT OVARY (OV) : -CYST,-PRESENT 3538 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1777 ANIMAL NUMBER: C93078 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 597.4 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 12:58 PROSECTOR: JERALD W. JOHNSON RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 7 MM IN DIAMETER, DARK RED AND SOFT MAMMARY, FEMALE (MF) : -MASS(ES); LEFT AND RIGHT INGUINAL REGIONS: TWO MASSES, LEFT INGUINAL IS 50 X 46 X 30 MM, RIGHT INGUINAL IS 80 X 55 X 30 MM, BOTH ARE RED, TAN, SOFT AND LOBULATED; BOTH ARE SAME ON CUT SURFACE; BOTH ON SECTION EXUDE A TAN CREAMY SEMIFLUID; RIGHT INGUINAL COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT -B-FIBROADENOMA,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 3539 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1778 ANIMAL NUMBER: C93078 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 597.4 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 12:58 PROSECTOR: JERALD W. JOHNSON RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), THYROID (TY), PARATHYROID (PT), URINARY BLADDER (UB) 3540 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1779 ANIMAL NUMBER: C93079 SEX: FEMALE DATE OF DEATH: 04/20/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/20/00 8:30 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 577.0 GRAMS PROSECTOR: JODI THIEREN RECORDER: JODI THIEREN PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); AXILLARY REGION-RIGHT; WEEK 69; 70 X 80MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); AXILLARY REGION-LEFT; WEEK 101; 10 X 10MM; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -LARGE; ALL LOBES: MARGINS APPEAR ROUNDED MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT AXILLARY REGION: SINGLE MASS, 90 X 75 X 40 MM, RED, TAN, LOBULATED, FIRM AND VASCULAR; SAME ON CUT SURFACE; PORTION COLLECTED; REGIONAL LN (AXILLARY) NORMAL (CAP 22) -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION HISTOPATHOLOGY LIVER (LI) : -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-MODERATE -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MODERATE -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MODERATELY SEVERE -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, PELVIC,-MODERATELY SEVERE THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL MAMMARY, FEMALE (MF) : -B-FIBROADENOMA,- 2 NEOPLASMS PRESENT -HYPERPLASIA, GLANDULAR,-SEVERE 3541 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1780 ANIMAL NUMBER: C93079 SEX: FEMALE DATE OF DEATH: 04/20/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/20/00 8:30 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 577.0 GRAMS PROSECTOR: JODI THIEREN RECORDER: JODI THIEREN PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS #2; LEFT AXILLARY REGION: SINGLE MASS, 20 X 17 X 15 MM, TAN AND FIRM; SAME ON CUT SURFACE; REGIONAL LN (AXILLARY) NORMAL (CAP 22) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, AXILLARY (AX) : -HYPERPLASIA, LYMPHOPLASMACYTIC,MODERATE 3542 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1781 ANIMAL NUMBER: C93079 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 577.0 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 8:30 PROSECTOR: JODI THIEREN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), LUNG (LU), PARATHYROID (PT), URINARY BLADDER (UB) 3543 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1782 ANIMAL NUMBER: C93080 SEX: FEMALE DATE OF DEATH: 04/21/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/21/00 7:49 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 510.7 GRAMS PROSECTOR: JAMES T. CALLEN RECORDER: LAURIE J. SCHULLER PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) OVARY (OV) : -CYST(S); LEFT: SINGLE CLEAR FLUID FILLED CYST, 5 MM IN DIAMETER PITUITARY (PI) : -MASS(ES); 7 X 5 X 4 MM, DARK RED, TAN, AND FIRM GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -GRANULAR CYTOPLASM, EOSINOPHILIC, CENTRILOBULAR,-MINIMAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-SLIGHT PANCREAS (PA) : -INFILTRATION, FAT, INTERSTITIAL,MINIMAL OVARY (OV) : -CYST,-PRESENT LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL PITUITARY (PI) : -HYPERPLASIA,-MODERATE MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 3544 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1783 ANIMAL NUMBER: C93080 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 510.7 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 7:49 PROSECTOR: JAMES T. CALLEN RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: THYROID (TY), PARATHYROID (PT), URINARY BLADDER (UB) 3545 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1784 ANIMAL NUMBER: C93081 SEX: FEMALE DATE OF DEATH: 04/20/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/20/00 11:00 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 453.5 GRAMS PROSECTOR: TROY ELLENBECKER RECORDER: JERALD W. JOHNSON PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -LIGHT FOCUS(I)/AREA(S); AT JUNCTION OF RIGHT AND LEFT MEDIAN LOBES: SINGLE TAN AREA, 5 X 2 MM (CAP 22) -MASS(ES); LEFT LATERAL LOBE: SINGLE MASS, 20 X 15 X 11 MM, RED, DARK RED, BROWN AND SOFT; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (HEPATIC) NOT IDENTIFIED -RED FOCUS(I)/AREA(S); RIGHT MEDIAN LOBE: SINGLE DARK RED AREA, 2 X 1 MM; COLLECTED ON ROUTINE SECTION LUNG (LU) : -LIGHT FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE TAN AREAS, UP TO 3 X 2 MM MUSCLE, SKELETAL (SM) : -ATROPHY-FOCAL; CRANIAL ONE HALF: 10 X 4 X 2 MM HISTOPATHOLOGY LIVER (LI) : -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MODERATE -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-SLIGHT -B-ADENOMA, HEPATOCELLULAR,-NEOPLASM PRESENT -LIPIDOSIS, SUBCAPSULAR (TENSION LIPIDOSIS),-MODERATE KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL PANCREAS (PA) : -INFILTRATION, FAT, INTERSTITIAL,MINIMAL LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,MODERATE MUSCLE, SKELETAL (SM) : >UNREMARKABLE 3546 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1785 ANIMAL NUMBER: C93081 SEX: FEMALE DATE OF DEATH: 04/20/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/20/00 11:00 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 453.5 GRAMS PROSECTOR: TROY ELLENBECKER RECORDER: JERALD W. JOHNSON PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY UTERUS (UT) : -MASS(ES); WITHIN LUMEN OF LEFT HORN: SINGLE MASS, 20 X 7 X 4 MM, RED, DARK RED AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (LUMBAR) NORMAL (CAP 24) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN MAMMARY, FEMALE (MF) : -HYPERPLASIA, GLANDULAR,-SLIGHT UTERUS (UT) : -DILATATION,-MODERATELY SEVERE -HEMORRHAGE,-PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 3547 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1786 ANIMAL NUMBER: C93081 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 453.5 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 11:00 PROSECTOR: TROY ELLENBECKER RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: THYROID (TY), PARATHYROID (PT), URINARY BLADDER (UB), LN, LUMBAR/ILIAC (LM) 3548 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1787 ANIMAL NUMBER: C93082 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 02/25/00 STUDY DAY OF DEATH: 677 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 02/25/00 9:46 PROSECTOR: NANCY DIEDRICH RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >EYES; PALE; EYES; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; YELLOW SKIN; PAWS-ALL; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; YELLOW SKIN; TAIL; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; YELLOW HAIR COAT; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -RED FOCUS(I)/AREA(S); DORSAL SURFACE OF BOTH CEREBRAL HEMISPHERES: MULTIPLE DARK RED PINPOINT FOCI LIVER (LI) : -LARGE; ALL LOBES: MARGINS ROUNDED, LOBES THICKENED -MOTTLED; ALL LOBES: RED AND TAN -LIGHT FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE TAN SLIGHTLY RAISED AREAS, UP TO 3 MM IN DIAMETER; COLLECTED ON ROUTINE SECTIONS -ROUGH SURFACE, DIFFUSE; ALL LOBES -CYST(S); ALL LOBES: MULTIPLE CLEAR FLUID FILLED CYSTS, UP TO 5 MM IN DIAMETER (CAP 22) KIDNEY (KD) : -DIFFUSELY DARK; BILATERAL: DARK BROWN SPLEEN (SP) : -LARGE; 6 5 X 2 0 X 1 0 MM -ADHESION(S); BOTH SURFACES OF CAUDAL END: MULTIPLE TAN FIBROUS ADHESIONS; COLLECTED INTACT HISTOPATHOLOGY BRAIN (BR) : >UNREMARKABLE LIVER (LI) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT -HEMORRHAGE,-PRESENT -CYST, BILIARY,-PRESENT KIDNEY (KD) : -MINERALIZATION, PELVIC,-SLIGHT -PIGMENT, TUBULAR,-SEVERE SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT 3549 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1788 ANIMAL NUMBER: C93082 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 02/25/00 STUDY DAY OF DEATH: 677 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 02/25/00 9:46 PROSECTOR: NANCY DIEDRICH RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY LN, MESENTERIC (MS) : -LARGE; UP TO 8 MM IN DIAMETER -DIFFUSELY RED OVARY (OV) : -CYST(S); LEFT: SINGLE CLEAR FLUID FILLED CYST, 2 MM IN DIAMETER LUNG (LU) : -RED FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE DARK RED AREAS, UP TO 5 X 4 MM PITUITARY (PI) : -RED FOCUS(I)/AREA(S); RIGHT POSTERIOR ASPECT: TWO DARK RED FOCI, ONE IS 2 MM IN DIAMETER, THE OTHER IS 1 MM IN DIAMETER PANCREAS (PA) : -ATROPHY, ACINAR,-MINIMAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT -PIGMENT,-MINIMAL LN, MESENTERIC (MS) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT OVARY (OV) : -CYST,-PRESENT THYMUS (TH) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT LUNG (LU) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT -MINERALIZATION, VASCULAR,-MINIMAL -HEMORRHAGE,-PRESENT -INFILTRATE, MACROPHAGE, PIGMENTED,MODERATE PITUITARY (PI) : -HYPERPLASIA,-MINIMAL STOMACH, GL (ST) : -EROSION,-MINIMAL 3550 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1789 ANIMAL NUMBER: C93082 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 02/25/00 STUDY DAY OF DEATH: 677 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 02/25/00 9:46 PROSECTOR: NANCY DIEDRICH RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: MULTIPLE DARK BROWN AREAS, UP TO 4 X 2 MM ILEUM (IL) : -DIVERTICULUM(A); SINGLE DIVERTICULUM, 8 X 5 X 3 MM (CAP 24) LN, MEDIASTINAL (ML) : -DIFFUSELY RED; DARK RED (CAP 21) LN, LUMBAR/ILIAC (LM) : -LARGE; BILATERAL: EACH IS UP TO 8 X 5 X 3 MM (CAP 23) ILEUM (IL) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT CECUM (CE) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT COLON (CO) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -HYPERPLASIA, GLANDULAR,-MINIMAL CERVIX (CV) : -HYPERPLASIA, MUCINOUS,-SLIGHT VAGINA (VA) : -HYPERPLASIA, MUCINOUS,-SLIGHT HEMATO NEOPLASIA (HN) : -M-SARCOMA, HISTIOCYTIC,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, HEMATOPOIETIC,-PRESENT LN, MEDIASTINAL (ML) : -HEMORRHAGE,-PRESENT LN, LUMBAR/ILIAC (LM) : -HEMORRHAGE,-PRESENT 3551 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1790 ANIMAL NUMBER: C93082 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 02/25/00 STUDY DAY OF DEATH: 677 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 02/25/00 9:46 PROSECTOR: NANCY DIEDRICH RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY LN, LUMBAR/ILIAC (LM) : -DIFFUSELY RED; BILATERAL: DARK RED (CAP 23) LN, ILEOCECAL (IC) : -LARGE; 8 X 5 X 3 MM (CAP 25) -DIFFUSELY RED; (CAP 25) GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; YELLOW MOIST MATERIAL -STAINS - SKIN/HAIRCOAT; VENTRAL ABDOMINAL AND THORACIC REGIONS: YELLOW AND DRY MATERIAL -YELLOWISH MUCOUS MEMBRANES; ALL AREAS, INCLUDING EXTREMITIES LN, ILEOCECAL (IC) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT 3552 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1791 ANIMAL NUMBER: C93082 SEX: FEMALE DATE OF DEATH: 02/25/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 02/25/00 9:46 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DEATH: 677 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: NOT ENTERED PROSECTOR: NANCY DIEDRICH RECORDER: TONE EVERTS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM JEJUNUM (JE), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT) (SB), (DU), 3553 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1792 ANIMAL NUMBER: C93083 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/17/00 STUDY DAY OF DEATH: 729 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 491.1 GRAMS DATE AND TIME OF NECROPSY: 04/17/00 14:57 PROSECTOR: JODI THIEREN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); VENTRAL-CERVICAL-LEFT; WEEK 87; 100 X 90MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); VENTRAL-ABDOMINAL; WEEK 95; 15 X 10MM; VERIFIED AT NECROPSY: NOT OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) ADRENAL, CORTEX (AC) : -LARGE; LEFT: 12 X 9 X 9 MM (RIGHT IS 7 X 6 X 5 MM) HISTOPATHOLOGY BRAIN (BR) : >UNREMARKABLE LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MODERATELY SEVERE -HYPERTROPHY, HEPATOCELLULAR, PERIPORTAL,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, PELVIC,-MODERATELY SEVERE SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-MODERATE ADRENAL, CORTEX (AC) : -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL -HYPERTROPHY, CORTICAL CELL,-SEVERE -DEGENERATION, CYSTIC / HEMORRHAGIC,SEVERE MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,-SEVERE 3554 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1793 ANIMAL NUMBER: C93083 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/17/00 STUDY DAY OF DEATH: 729 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 491.1 GRAMS DATE AND TIME OF NECROPSY: 04/17/00 14:57 PROSECTOR: JODI THIEREN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 6 X 5 X 4 MM, RED, DARK RED, GREY AND FIRM MAMMARY, FEMALE (MF) : -MASS(ES); VENTRAL CERVICAL EXTENDING TO VENTRAL THORACIC: SINGLE MASS, 120 X 110 X 50 MM (209.3 GRAMS), RED, DARK RED, TAN, GREY, LOBULATED FIRM, SOFT, AND VASCULAR; ON SECTION SOME AREAS EXUDE A RED FLUID, OTHERS EXUDE A TAN CASEOUS SEMISOLID; PORTION COLLECTED; OVERLYING SKIN HAS A DARK RED CRUSTED AREA, 65 X 35 MM; COLLECT INTACT WITH MASS; REGIONAL LN (MANDIBULAR) NORMAL (CAP 21) MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,-SEVERE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATELY SEVERE LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,SLIGHT HEART (HT) : -CARDIOMYOPATHY,-SLIGHT THYROID (TY) : -HYPERPLASIA, C-CELL,-SLIGHT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT -B-FIBROADENOMA,-NEOPLASM PRESENT -HYPERPLASIA, GLANDULAR,-SLIGHT 3555 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1794 ANIMAL NUMBER: C93083 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/17/00 STUDY DAY OF DEATH: 729 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 491.1 GRAMS DATE AND TIME OF NECROPSY: 04/17/00 14:57 PROSECTOR: JODI THIEREN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) -BLOOD FILM TAKEN -ANIMAL THIN CAUSE OF DEATH (CD) : -NEOPLASM, MAMMARY GLAND,-PRESENT LN, MANDIBULAR (MN) : -HYPERPLASIA, LYMPHOPLASMACYTIC,MODERATE 3556 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1795 ANIMAL NUMBER: C93083 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/17/00 STUDY DAY OF DEATH: 729 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 491.1 GRAMS DATE AND TIME OF NECROPSY: 04/17/00 14:57 PROSECTOR: JODI THIEREN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 3557 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1796 ANIMAL NUMBER: C93084 SEX: FEMALE DATE OF DEATH: 05/15/98 STUDY DAY OF DATE AND TIME OF NECROPSY: 05/15/98 10:26 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 186.0 GRAMS PROSECTOR: JODI THIEREN RECORDER: DEBBIE PIRKEL PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN STATUS LF ADRENAL (AD0) RT ADRENAL (AD1) BRAIN (BR) LF KIDNEY (KD0) RT KIDNEY (KD1) LIVER (LI) LUNG (LU) LF OVARY (OV0) RT OVARY (OV1) SPLEEN (SP) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) UTERUS (UT) ------------------------------------ 6.9911 -------------------------------------------------- ------------------------------------ 3.7584 % -------------------------------------------------- -------------------------------------------------------------------------------------------- NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN WEIGHT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS 3558 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1797 ANIMAL NUMBER: C93084 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 186.0 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 10:26 PROSECTOR: JODI THIEREN RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) 3559 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1798 ANIMAL NUMBER: C93085 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 268.5 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 12:59 PROSECTOR: TROY ELLENBECKER RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) LF ADRENAL (AD0) RT ADRENAL (AD1) BRAIN (BR) LF KIDNEY (KD0) RT KIDNEY (KD1) LIVER (LI) LUNG (LU) LF OVARY (OV0) RT OVARY (OV1) SPLEEN (SP) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) UTERUS (UT) .0315 .0337 2.1358 1.1824 1.2842 9.0653 1.1579 .0745 .0707 .4904 .0080 .0106 .5732 .0117 % .0126 % .7955 % .4404 % .4783 % 3.3763 % .4312 % .0277 % .0263 % .1826 % .0030 % .0039 % .2135 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0147 .0158 1.0000 .5536 .6013 4.2445 .5421 .0349 .0331 .2296 .0037 .0050 .2684 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 3560 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1799 ANIMAL NUMBER: C93085 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 268.5 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 12:59 PROSECTOR: TROY ELLENBECKER RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV) 3561 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1800 ANIMAL NUMBER: C93086 SEX: FEMALE DATE OF DEATH: 01/09/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 01/09/00 9:26 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 630 STUDY WEEK OF DEATH: 90 TERMINAL BODY WEIGHT: 351.3 GRAMS PROSECTOR: DONIA MEYER RECORDER: DONIA MEYER PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK 76; 60 X 50MM; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: NOT OBSERVED >APPEARANCE; HEAD TILT; LEFT; VERIFIED AT NECROPSY: NOT APPLICABLE >EYES; DILATED PUPIL; EYES; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; ATAXIC; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 15 X 10 X 8 MM, RED, DARK RED, TAN AND SOFT MAMMARY, FEMALE (MF) : -MASS(ES); LEFT INGUINAL REGION: SINGLE MASS, 70 X 50 X 20 MM, RED, DARK RED, TAN, VASCULAR AND SOFT; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED HISTOPATHOLOGY BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -DEGENERATION, CYSTIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-SLIGHT -FIBROSIS, PERIBILIARY,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, PELVIC,-MODERATE SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE ADRENAL, MEDULLA (MA) : -B-PHEOCHROMOCYTOMA,-NEOPLASM PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -B-FIBROADENOMA,-NEOPLASM PRESENT 3562 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1801 ANIMAL NUMBER: C93086 SEX: FEMALE DATE OF DEATH: 01/09/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 01/09/00 9:26 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 630 STUDY WEEK OF DEATH: 90 TERMINAL BODY WEIGHT: 351.3 GRAMS PROSECTOR: DONIA MEYER RECORDER: DONIA MEYER PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) -BLOOD FILM TAKEN CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, CORTEX (AC), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 3563 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1802 ANIMAL NUMBER: C93087 SEX: FEMALE DATE OF DEATH: 03/16/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 03/16/00 10:42 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 697 STUDY WEEK OF DEATH: 100 TERMINAL BODY WEIGHT: 308.1 GRAMS PROSECTOR: TODD ACKER RECORDER: TODD ACKER PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); INGUINAL REGION-LEFT; WEEK 93; 40 X 30MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); INGUINAL REGION-RIGHT; WEEK 95; 20 X 20MM; VERIFIED AT NECROPSY: OBSERVED >EYES; RED DISCHARGE; EYES; VERIFIED AT NECROPSY: OBSERVED >DISCHARGE; NASAL; RED IN COLOR; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; ATAXIC; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) HISTOPATHOLOGY BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-SLIGHT -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, PELVIC,-MODERATE -HYPERPLASIA, TRANSITIONAL CELL,-SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT -HYPERTROPHY, CORTICAL CELL,-MINIMAL ADRENAL, MEDULLA (MA) : -HYPERPLASIA, MEDULLARY,-MINIMAL 3564 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1803 ANIMAL NUMBER: C93087 SEX: FEMALE DATE OF DEATH: 03/16/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 03/16/00 10:42 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 697 STUDY WEEK OF DEATH: 100 TERMINAL BODY WEIGHT: 308.1 GRAMS PROSECTOR: TODD ACKER RECORDER: TODD ACKER PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY OVARY (OV) : -CYST(S); RIGHT: SINGLE CLEAR FLUID FILLED CYST, 10 MM IN DIAMETER PITUITARY (PI) : -MASS(ES); 12 X 9 X 7 MM, RED, DARK RED, TAN, SOFT AND LOBULATED MAMMARY, FEMALE (MF) : -MASS(ES); LEFT INGUINAL REGION: SINGLE MASS, 40 X 35 X 25 MM, TAN, RED AND SOFT; CUT SURFACE EXUDES A TAN SEMIFLUID; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED -MASS #2; RIGHT INGUINAL REGION: SINGLE MASS, 28 X 25 X 15 MM, TAN, RED AND FIRM; CUT SURFACE EXUDES A SMALL AMOUNT OF A TAN SEMIFLUID; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED OVARY (OV) : -CYST,-PRESENT SPINAL CORD (SC) : -DEMYELINATION,-SLIGHT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY) SLIGHT HEART (HT) : -CARDIOMYOPATHY,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT -B-FIBROADENOMA,- 2 NEOPLASMS PRESENT UTERUS (UT) : -HYPERPLASIA, CYSTIC, ENDOMETRIAL,MINIMAL 3565 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1804 ANIMAL NUMBER: C93087 SEX: FEMALE DATE OF DEATH: 03/16/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 03/16/00 10:42 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 697 STUDY WEEK OF DEATH: 100 TERMINAL BODY WEIGHT: 308.1 GRAMS PROSECTOR: TODD ACKER RECORDER: TODD ACKER PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY LN, MANDIBULAR (MN) : -MOTTLED; BILATERAL: RED AND TAN; COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DARK RED AND DRY -DISCHARGE-NASAL; DARK RED AND DRY VAGINA (VA) : -LUMEN, EXUDATE,-PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT LN, MANDIBULAR (MN) : -HYPERPLASIA, LYMPHOPLASMACYTIC,MODERATE -HEMORRHAGE,-PRESENT 3566 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1805 ANIMAL NUMBER: C93087 SEX: FEMALE DATE OF DEATH: 03/16/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 03/16/00 10:42 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 697 STUDY WEEK OF DEATH: 100 TERMINAL BODY WEIGHT: 308.1 GRAMS PROSECTOR: TODD ACKER RECORDER: TODD ACKER PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), HEMATO NEOPLASIA (HN) 3567 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1806 ANIMAL NUMBER: C93088 SEX: FEMALE DATE OF DEATH: 10/15/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 10/15/99 13:23 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DEATH: 544 STUDY WEEK OF DEATH: 78 TERMINAL BODY WEIGHT: NOT ENTERED PROSECTOR: JOHN S. HALFORD RECORDER: JARED FISHEL PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; ROUGH HAIR COAT; UNVERIFIED AT NECROPSY >APPEARANCE; HEAD TILT; LEFT; UNVERIFIED AT NECROPSY >SKIN & PELAGE; RED HAIR COAT; HEADENTIRE; UNVERIFIED AT NECROPSY OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) ADRENAL, CORTEX (AC) : >NOTE:>TRIMMING COMMENT: ONE ADRENAL MISSING FROM WET TISSUE ADRENAL, MEDULLA (MA) : >NOTE:>TRIMMING COMMENT: ONE ADRENAL MISSING FROM WET TISSUE HISTOPATHOLOGY EYE (EY) : -DYSTROPHY, CORNEAL IN EARLY STAGES, CONSISTS PRIMARILY OF FOCAL MINERALIZATION,-SLIGHT BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-SLIGHT -HYPERPLASIA, BILE DUCT,-MINIMAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : -UNILATERALLY EXAMINED,-PRESENT -ANGIECTASIS,-SLIGHT -PIGMENT,-MINIMAL ADRENAL, MEDULLA (MA) : -UNILATERALLY EXAMINED,-PRESENT MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,-MODERATE HEART (HT) : -CARDIOMYOPATHY,-MINIMAL 3568 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1807 ANIMAL NUMBER: C93088 SEX: FEMALE DATE OF DEATH: 10/15/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 10/15/99 13:23 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DEATH: 544 STUDY WEEK OF DEATH: 78 TERMINAL BODY WEIGHT: NOT ENTERED PROSECTOR: JOHN S. HALFORD RECORDER: JARED FISHEL PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 12 X 10 X 9 MM, RED, DARK RED, GRAY, LOBULATED, AND SOFT UTERUS (UT) : -CYST(S); BILATERAL, WITHIN WALLS: MULTIPLE CLEAR FLUID FILLED CYSTS IN EACH, UP TO 8 MM IN DIAMETER GENERAL COMMENT (GC) : -MALOCCLUSION; BILATERAL, UPPER AND LOWER INCISORS PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT ILEUM (IL) : >AUTOLYTIC MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT UTERUS (UT) : -DILATATION,-MODERATE CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 3569 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1808 ANIMAL NUMBER: C93088 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 10/15/99 STUDY DAY OF DEATH: 544 STUDY WEEK OF DEATH: 78 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 10/15/99 13:23 PROSECTOR: JOHN S. HALFORD RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 3570 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1809 ANIMAL NUMBER: C93089 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/04/00 STUDY DAY OF DEATH: 656 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: 348.1 GRAMS DATE AND TIME OF NECROPSY: 02/04/00 8:03 PROSECTOR: TONY HANFELD RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK 87; 15 X 15MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); VENTRAL-CERVICAL; WEEK 88; 25 X 25MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); AXILLARY REGION-RIGHT; WEEK 92; 15 X 15MM; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; HEAD TILT; LEFT; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LN, MESENTERIC (MS) : -DIFFUSELY RED; CAUDAL END OF CHAIN: DARK RED HISTOPATHOLOGY BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-SLIGHT -GRANULAR CYTOPLASM, EOSINOPHILIC, CENTRILOBULAR,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATE -HYPERTROPHY, CORTICAL CELL,-MINIMAL -DEGENERATION, CYSTIC / HEMORRHAGIC,MINIMAL -HYPERPLASIA, CORTICAL,-SLIGHT LN, MESENTERIC (MS) : -B-HEMANGIOMA,-NEOPLASM PRESENT 3571 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1810 ANIMAL NUMBER: C93089 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/04/00 STUDY DAY OF DEATH: 656 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: 348.1 GRAMS DATE AND TIME OF NECROPSY: 02/04/00 8:03 PROSECTOR: TONY HANFELD RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY OVARY (OV) : -CYST(S); RIGHT: SINGLE CLEAR FLUID FILLED CYST, 7 MM IN DIAMETER PITUITARY (PI) : -MASS(ES); 10 X 7 X 6 MM, DARK RED, TAN, AND SOFT MAMMARY, FEMALE (MF) : -MASS(ES); VENTRAL CERVICAL REGION: SINGLE MASS, 40 X 30 X 30 MM, RED, DARK RED, TAN, CYSTIC, FIRM, AND LOBULATED; SOME SECTIONS EXUDE A TAN CREAMY SEMIFLUID; COLLECTED; REGIONAL LN (MANDIBULAR) NORMAL (CAP 21) -MASS #2; RIGHT AXILLARY REGION: SINGLE MASS, 35 X 25 X 15 MM, RED, TAN, LOBULATED, AND FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (AXILLARY) NORMAL (CAP 22) OVARY (OV) : -CYST,-PRESENT THYMUS (TH) : >SECTION EXAMINED; TISSUE NOT PRESENT LUNG (LU) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT COLON (CO) : -PARASITISM,-PRESENT MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT -B-FIBROADENOMA,- 2 NEOPLASMS PRESENT -HYPERPLASIA, GLANDULAR,-SLIGHT -B-ADENOMA,-NEOPLASM PRESENT >NOTE:>MASS #3 IS THE ADENOMA 3572 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1811 ANIMAL NUMBER: C93089 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/04/00 STUDY DAY OF DEATH: 656 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: 348.1 GRAMS DATE AND TIME OF NECROPSY: 02/04/00 8:03 PROSECTOR: TONY HANFELD RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS #3; LEFT VENTRAL ABDOMINAL REGION: SINGLE MASS, 25 X 15 X 12 MM, DARK BROWN, TAN, AND FIRM; SOME SECTIONS EXUDE A BROWN CREAMY SEMIFLUID; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED UTERUS (UT) : -CYST(S); BILATERAL, WITHIN WALLS: MULTIPLE CLEAR FLUID FILLED CYSTS, UP TO 5 MM IN DIAMETER GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL: UPPER INCISORS UTERUS (UT) : -HYPERPLASIA, CYSTIC, ENDOMETRIAL,MODERATELY SEVERE VAGINA (VA) : -LUMEN, EXUDATE,-PRESENT -HYPERPLASIA, MUCINOUS,-SLIGHT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT LN, AXILLARY (AX) : -HYPERPLASIA, LYMPHOPLASMACYTIC,-SLIGHT -INFILTRATE, MACROPHAGE, PIGMENTED,SLIGHT LN, MANDIBULAR (MN) : -HYPERPLASIA, LYMPHOPLASMACYTIC,MODERATELY SEVERE 3573 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1812 ANIMAL NUMBER: C93089 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/04/00 STUDY DAY OF DEATH: 656 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: 348.1 GRAMS DATE AND TIME OF NECROPSY: 02/04/00 8:03 PROSECTOR: TONY HANFELD RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), HEMATO NEOPLASIA (HN) 3574 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1813 ANIMAL NUMBER: C93090 SEX: FEMALE DATE OF DEATH: 04/19/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/19/00 12:53 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 423.1 GRAMS PROSECTOR: CHANESTA ACKER RECORDER: TERRI L. WILLIAMS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -DARK FOCUS(I)/AREA(S); RIGHT MEDIAN LOBE: MULTIPLE DARK FOCI, UP TO 3 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION PITUITARY (PI) : -MASS(ES); 8 X 7 X 5 MM, DARK RED, RED, TAN AND SOFT MAMMARY, FEMALE (MF) : -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION CERVIX (CV) : -LARGE; 12 X 8 X 5 MM HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-SLIGHT -GRANULAR CYTOPLASM, EOSINOPHILIC, CENTRILOBULAR,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, PELVIC,-MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-MODERATE PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -HYPERPLASIA, GLANDULAR,-MODERATELY SEVERE CERVIX (CV) : -HYPERPLASIA, MUCINOUS,-MODERATE VAGINA (VA) : -HYPERPLASIA, MUCINOUS,-SEVERE 3575 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1814 ANIMAL NUMBER: C93090 SEX: FEMALE DATE OF DEATH: 04/19/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/19/00 12:53 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 423.1 GRAMS PROSECTOR: CHANESTA ACKER RECORDER: TERRI L. WILLIAMS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY VAGINA (VA) : -MASS(ES); WITHIN LUMEN: SINGLE MASS, 7 X 5 X 3 MM, TAN, SOFT AND PEDUNCULATED; ON SECTION EXUDES A WHITE CREAMY SEMIFLUID; COLLECTED INTACT; REGIONAL LN (LUMBAR) NORMAL (CAP 22) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 3576 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1815 ANIMAL NUMBER: C93090 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 423.1 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 12:53 PROSECTOR: CHANESTA ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), LUNG (LU), PARATHYROID (PT), URINARY BLADDER (UB), LN, LUMBAR/ILIAC (LM) 3577 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1816 ANIMAL NUMBER: C93091 SEX: FEMALE DATE OF DEATH: 10/06/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 10/06/99 13:16 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 535 STUDY WEEK OF DEATH: 77 TERMINAL BODY WEIGHT: 421.0 GRAMS PROSECTOR: JODI THIEREN RECORDER: LAURIE J. SCHULLER PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); LIMB-HIND-RIGHT; WEEK 47; 100 X 100MM; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; LATERALABDOMINAL-RIGHT; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; PALE; ENTIRE BODY; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY LIVER (LI) : -ROUGH SURFACE, DIFFUSE; ALL LOBES -DIFFUSELY LIGHT; ALL LOBES: TAN KIDNEY (KD) : -MOTTLED; BILATERAL: RED AND TAN SPLEEN (SP) : -LARGE; 55 X 15 X 8 MM LN, MESENTERIC (MS) : -MOTTLED; DARK RED AND TAN OVARY (OV) : -CYST(S); RIGHT: SINGLE CLEAR FLUID FILLED CYST, 2 MM IN DIAMETER HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -HEMATOPOIESIS, EXTRAMEDULLARY,MODERATE -DEGENERATION / NECROSIS, CENTRILOBULAR,-MODERATE KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, PELVIC,-MINIMAL -MINERALIZATION, TUBULAR,-MINIMAL -HEMATOPOIESIS, EXTRAMEDULLARY,-SLIGHT -VACUOLATION, TUBULAR EPITHELIUM,MODERATE SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SEVERE ADRENAL, CORTEX (AC) : -HEMATOPOIESIS, EXTRAMEDULLARY,-SEVERE LN, MESENTERIC (MS) : -HEMORRHAGE,-PRESENT OVARY (OV) : >UNREMARKABLE 3578 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1817 ANIMAL NUMBER: C93091 SEX: FEMALE DATE OF DEATH: 10/06/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 10/06/99 13:16 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 535 STUDY WEEK OF DEATH: 77 TERMINAL BODY WEIGHT: 421.0 GRAMS PROSECTOR: JODI THIEREN RECORDER: LAURIE J. SCHULLER PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY LUNG (LU) : -MASS(ES); ALL LOBES: MULTIPLE MASSES, UP TO 7 X 7 X 6 MM, TAN AND FIRM; SAME ON CUT SURFACES; COLLECTED INTACT; REGIONAL LN (TRACHEOBRONCHIAL) NOT IDENTIFIED HEART (HT) : -LARGE; 25 X 15 X 11 MM -DIFFUSELY LIGHT; DIFFUSE: GRAY PITUITARY (PI) : -LARGE; 5 X 5 X 3 MM; APPEARS SWOLLEN -DARK FOCUS(I)/AREA(S); MID ASPECT: SINGLE DARK BROWN AREA, 4 X 2 MM MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,MODERATELY SEVERE MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,-MODERATE -HYPERPLASIA, ERYTHROID, MARROW,MODERATE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATELY SEVERE LUNG (LU) : -N-CARCINOMA,-NEOPLASM PRESENT HEART (HT) : >UNREMARKABLE PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL MAMMARY, FEMALE (MF) : -M-CARCINOMA,-NEOPLASM PRESENT 3579 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1818 ANIMAL NUMBER: C93091 SEX: FEMALE DATE OF DEATH: 10/06/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 10/06/99 13:16 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 535 STUDY WEEK OF DEATH: 77 TERMINAL BODY WEIGHT: 421.0 GRAMS PROSECTOR: JODI THIEREN RECORDER: LAURIE J. SCHULLER PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT LATERAL LUMBAR REGION: SINGLE MASS, 98 X 75 X 68 MM, RED, DARK RED, TAN, FIRM, AND VASCULAR; ON SECTION EXUDES A DARK RED FLUID AND A YELLOW SEMIFLUID; PORTION COLLECTED; OVERLYING SKIN HAS A DARK RED MOIST ULCERATED AREA, 25 MM IN DIAMETER; COLLECTED INTACT WITH PORTION OF MASS COLLECTED; REGIONAL LN (SEE AXILLARY) -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION UTERUS (UT) : -CYST(S); BILATERAL WITHIN WALLS: MULTIPLE CLEAR FLUID FILLED CYSTS, UP TO 7 X 3 X 3 MM LN, AXILLARY (AX) : -MOTTLED; BILATERAL: DARK RED AND TAN (CAP 21) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN UTERUS (UT) : -DILATATION,-MODERATE CAUSE OF DEATH (CD) : -NEOPLASM, MAMMARY GLAND,-PRESENT LN, AXILLARY (AX) : >UNREMARKABLE 3580 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1819 ANIMAL NUMBER: C93091 SEX: FEMALE DATE OF DEATH: 10/06/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 10/06/99 13:16 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 535 STUDY WEEK OF DEATH: 77 TERMINAL BODY WEIGHT: 421.0 GRAMS PROSECTOR: JODI THIEREN RECORDER: LAURIE J. SCHULLER PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) (CE), 3581 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1820 ANIMAL NUMBER: C93092 SEX: FEMALE DATE OF DEATH: 10/06/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 10/06/99 11:30 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 535 STUDY WEEK OF DEATH: 77 TERMINAL BODY WEIGHT: 309.7 GRAMS PROSECTOR: JODI THIEREN RECORDER: LAURIE J. SCHULLER PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); AXILLARY REGION-RIGHT; WEEK 72; 10 X 10MM; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; HEAD TILT; LEFT; VERIFIED AT NECROPSY: NOT APPLICABLE >EYES; RED DISCHARGE; EYES; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; ATAXIC; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) ADRENAL, CORTEX (AC) : -LARGE; RIGHT: 8 X 6 X 5 MM (LEFT IS 6 X 4 X 2 MM) -DARK FOCUS(I)/AREA(S); RIGHT CRANIAL END: SINGLE DARK BROWN AREA, 5 MM IN DIAMETER HISTOPATHOLOGY EYE (EY) : -ATROPHY, RETINAL,-SLIGHT BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-MINIMAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-SLIGHT -HYPERPLASIA, TRANSITIONAL CELL,-SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT PANCREAS (PA) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC / HEMORRHAGIC,MODERATELY SEVERE -PIGMENT,-MINIMAL 3582 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1821 ANIMAL NUMBER: C93092 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/06/99 STUDY DAY OF DEATH: 535 STUDY WEEK OF DEATH: 77 TERMINAL BODY WEIGHT: 309.7 GRAMS DATE AND TIME OF NECROPSY: 10/06/99 11:30 PROSECTOR: JODI THIEREN RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 11 X 9 X 9 MM, RED. DARK RED, TAN, GRAY, SOFT, AND LOBULATED MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT AXILLARY REGION: SINGLE MASS, 28 X 20 X 13 MM, TAN AND FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (AXILLARY) NORMAL (CAP 21) -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTIONS UTERUS (UT) : -THICKENED WALL; BILATERAL GENERAL COMMENT (GC) -BLOOD FILM TAKEN -DISCHARGE-OCULAR; AND DRY MATERIAL -ANIMAL THIN : BILATERAL: DARK RED THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MINIMAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT -B-FIBROADENOMA,-NEOPLASM PRESENT UTERUS (UT) : ^UNREMARKABLE VAGINA (VA) : -LUMEN, EXUDATE,-PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 3583 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1822 ANIMAL NUMBER: C93092 SEX: FEMALE DATE OF DEATH: 10/06/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 10/06/99 11:30 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 535 STUDY WEEK OF DEATH: 77 TERMINAL BODY WEIGHT: 309.7 GRAMS PROSECTOR: JODI THIEREN RECORDER: LAURIE J. SCHULLER PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), HEMATO NEOPLASIA (HN), LN, AXILLARY (AX) 3584 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1823 ANIMAL NUMBER: C93093 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/18/00 STUDY DAY OF DEATH: 670 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: 425.1 GRAMS DATE AND TIME OF NECROPSY: 02/18/00 10:20 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK 70; 70 X 70MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); VENTRAL-ABDOMINAL-RIGHT; WEEK 89; 45 X 45MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); AXILLARY REGION-LEFT; WEEK 91; 10 X 10MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); VENTRAL-CERVICAL-LEFT; WEEK 94; 15 X 15MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); VENTRAL-CERVICAL-RIGHT; WEEK 94; 10 X 10MM; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) ADRENAL, CORTEX (AC) : -LARGE; LEFT: 10 MM IN DIAMETER (RIGHT IS 5 MM IN DIAMETER) -CYST(S); LEFT: SINGLE CLEAR FLUID FILLED CYST, 6 MM IN DIAMETER HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, PELVIC,-MODERATE -HYPERPLASIA, TRANSITIONAL CELL,-SLIGHT >NOTE:>SINGLE SMALL AREA OF HEMORRHAGE WITH PROTEINACEOUS-APPEARING LAMELLAR SPHERULES. SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SLIGHT ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT -HEMORRHAGE,-PRESENT -HYPERTROPHY, CORTICAL CELL,-MODERATE -DEGENERATION, CYSTIC / HEMORRHAGIC,MINIMAL -PIGMENT,-SLIGHT 3585 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1824 ANIMAL NUMBER: C93093 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/18/00 STUDY DAY OF DEATH: 670 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: 425.1 GRAMS DATE AND TIME OF NECROPSY: 02/18/00 10:20 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); SINGLE MASS, 15 X 13 X 8 MM, DARK RED AND SOFT MAMMARY, FEMALE (MF) : -MASS(ES); LEFT INGUINAL REGION: SINGLE MASS, 90 X 65 X 30 MM, RED, TAN, VASCULAR, AND SOFT; SOME AREAS EXUDE A TAN CREAMY SEMISOLID; PORTION COLLECTED; REGIONAL LN (INGUINAL) NORMAL (CAP 21) -MASS #2; RIGHT INGUINAL REGION: SINGLE MASS, 40 X 40 X 20 MM, DARK RED, DARK BROWN, AND SOFT; ON SECTION EXUDES A CLEAR DARK BROWN FLUID; COLLECTED; REGIONAL LN (INGUINAL) NORMAL (CAP 21) MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,-MODERATE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MINIMAL LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL HEART (HT) : -MINERALIZATION, VASCULAR,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -M-CARCINOMA,- 2 NEOPLASMS PRESENT -CYST / GALACTOCELE,-PRESENT -B-FIBROADENOMA,- 2 NEOPLASMS PRESENT -HYPERPLASIA, GLANDULAR,-MODERATE -B-ADENOMA,-NEOPLASM PRESENT >NOTE:>MASSES 1 & 5 ARE FIBROADENOMAS MASSES 3 & 4 ARE CARCINOMAS MASS 2 IS AN ADENOMA 3586 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1825 ANIMAL NUMBER: C93093 SEX: FEMALE DATE OF DEATH: 02/18/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 02/18/00 10:20 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 670 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: 425.1 GRAMS PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS #3; LEFT AXILLARY REGION: SINGLE MASS, 25 X 15 X 10 MM, DARK BROWN, TAN, LOBULATED, AND FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (AXILLARY) NORMAL (CAP 22) -MASS #4; LEFT CERVICAL REGION: SINGLE MASS, 25 X 20 X 15 MM, TAN, FIRM AND VASCULAR; ON SECTION EXUDES A TAN AND GREEN CREAMY SEMIFLUID; COLLECTED; REGIONAL LN (MANDIBULAR) NORMAL (CAP 23) -MASS #5; RIGHT CERVICAL REGION: SINGLE MASS, 30 X 20 X 15 MM, TAN AND SOFT; ON SECTION EXUDES A YELLOW AND TAN CREAMY SEMIFLUID; COLLECTED; REGIONAL LN (MANDIBULAR) NORMAL (CAP 23) UTERUS (UT) : -HYPERPLASIA, CYSTIC, ENDOMETRIAL,MINIMAL CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT LN, AXILLARY (AX) : -HYPERPLASIA, LYMPHOPLASMACYTIC,MINIMAL LN, INGUINAL (IN) : -HYPERPLASIA, LYMPHOPLASMACYTIC,MINIMAL 3587 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1826 ANIMAL NUMBER: C93093 SEX: FEMALE DATE OF DEATH: 02/18/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 02/18/00 10:20 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 670 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: 425.1 GRAMS PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL, UPPER INCISORS -ANIMAL THIN LN, MANDIBULAR (MN) : -HYPERPLASIA, LYMPHOPLASMACYTIC,-SEVERE 3588 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1827 ANIMAL NUMBER: C93093 SEX: FEMALE DATE OF DEATH: 02/18/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 02/18/00 10:20 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 670 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: 425.1 GRAMS PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 3589 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1828 ANIMAL NUMBER: C93094 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/18/00 STUDY DAY OF DEATH: 670 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: 388.9 GRAMS DATE AND TIME OF NECROPSY: 02/18/00 7:45 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); VENTRAL-CERVICAL-RIGHT; WEEK 76; 55 X 60MM; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; VENTRALCERVICAL-RIGHT; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >EYES; RED DISCHARGE; EYES; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; COLD TO TOUCH; ENTIRE BODY; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -DIFFUSELY LIGHT; ALL LOBES: TAN -RED FOCUS(I)/AREA(S); DIAPHRAGMATIC SURFACE OF RIGHT MEDIAN LOBE: SINGLE RED FOCUS, 3 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION KIDNEY (KD) : -DIFFUSELY LIGHT; BILATERAL: TAN HISTOPATHOLOGY EYE (EY) : -INFLAMMATION, ACUTE,-SLIGHT BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-SLIGHT -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-MODERATE -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SLIGHT -MINERALIZATION, PELVIC,-SLIGHT -MINERALIZATION, TUBULAR,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT ADRENAL, CORTEX (AC) : -HYPERTROPHY, CORTICAL CELL,-MODERATE -DEGENERATION, CYSTIC / HEMORRHAGIC,MODERATE 3590 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1829 ANIMAL NUMBER: C93094 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/18/00 STUDY DAY OF DEATH: 670 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: 388.9 GRAMS DATE AND TIME OF NECROPSY: 02/18/00 7:45 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); SINGLE MASS, 10 X 9 X 6 MM, DARK RED, TAN, AND SOFT MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT CERVICAL REGION: SINGLE MASS, 60 X 40 X 30 MM, RED, DARK RED, TAN, LOBULATED, VASCULAR, AND FIRM; SOME AREAS EXUDE A DARK BROWN SEMISOLID; COLLECTED; REGIONAL LN (MANDIBULAR) NORMAL (CAP 21); OVERLYING SKIN OF MASS HAS A SINGLE DARK BROWN, DRY CRUSTED AREA, 12, X 10 MM; COLLECTED INTACT WITH MASS -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION ADRENAL, MEDULLA (MA) : -HYPERPLASIA, MEDULLARY,-MODERATE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATELY SEVERE PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, NONGL (SU) : -ACANTHOSIS / HYPERKERATOSIS,-MODERATE -ULCERATION,-MINIMAL -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE MAMMARY, FEMALE (MF) : -M-CARCINOMA,-NEOPLASM PRESENT -CYST / GALACTOCELE,-PRESENT -HYPERPLASIA, GLANDULAR,-SLIGHT VAGINA (VA) : -HYPERPLASIA, MUCINOUS,-SLIGHT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 3591 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1830 ANIMAL NUMBER: C93094 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/18/00 STUDY DAY OF DEATH: 670 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: 388.9 GRAMS DATE AND TIME OF NECROPSY: 02/18/00 7:45 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DARK RED AND DRY LN, MANDIBULAR (MN) : -HYPERPLASIA, LYMPHOPLASMACYTIC,-SLIGHT 3592 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1831 ANIMAL NUMBER: C93094 SEX: FEMALE DATE OF DEATH: 02/18/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 02/18/00 7:45 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 670 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: 388.9 GRAMS PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), HEMATO NEOPLASIA (HN) 3593 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1832 ANIMAL NUMBER: C93095 SEX: FEMALE DATE OF DEATH: 04/17/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/17/00 16:05 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DEATH: 729 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: NOT ENTERED PROSECTOR: JODI THIEREN RECORDER: TONY HANFELD PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); AXILLARY REGION-LEFT; WEEK 79; 40 X 35MM; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; AXILLARY REGION-LEFT; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); VENTRAL-ABDOMINAL; WEEK 97; 20 X 20MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); INGUINAL REGION-LEFT; WEEK 97; 55 X 40MM; VERIFIED AT NECROPSY: OBSERVED >EYES; PALE; EYES; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; PALE; ENTIRE BODY; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; COLD TO TOUCH; ENTIRE BODY; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) HISTOPATHOLOGY BRAIN (BR) : >UNREMARKABLE LIVER (LI) : -VACUOLATION, HEPATOCELLULAR, MIDZONAL / CENTRILOBULAR,-MODERATELY SEVERE -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SLIGHT -MINERALIZATION, PELVIC,-SLIGHT SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SEVERE ADRENAL, CORTEX (AC) : -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL -HYPERTROPHY, CORTICAL CELL,-MODERATE -DEGENERATION, CYSTIC / HEMORRHAGIC,SLIGHT -PIGMENT,-MINIMAL ADRENAL, MEDULLA (MA) : -UNILATERALLY EXAMINED,-PRESENT OVARY (OV) : -CYST,-PRESENT 3594 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1833 ANIMAL NUMBER: C93095 SEX: FEMALE DATE OF DEATH: 04/17/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/17/00 16:05 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DEATH: 729 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: NOT ENTERED PROSECTOR: JODI THIEREN RECORDER: TONY HANFELD PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY THYMUS (TH) : -MISSING/NOT IDENTIFIED; DUE TO ADHESIONS, POSSIBLE REMNANTS SUBMITTED LUNG (LU) : -MASS(ES); ALL LOBES: MULTIPLE TAN, RED AND FIRM MASSES, UP TO 13 X 7 X 4 MM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (TRACHEOBRONCHIAL) NOT IDENTIFIED -ADHESION(S); ALL LOBES ADHERED TO EACH OTHER, DIAPHRAGM, HEART AND THYMUS: MULTIPLE GREY FIBROUS ADHESIONS; COLLECTED INTACT PITUITARY (PI) : -MASS(ES); 7 X 6 X 4 MM, RED , DARK RED, TAN AND FIRM MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,-SEVERE MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,-SEVERE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),SEVERE LUNG (LU) : -N-CARCINOMA,-FROM MAMMARY, FEMALE HEART (HT) : -CARDIOMYOPATHY,-MINIMAL -INFLAMMATION, CHRONIC-ACTIVE,MODERATELY SEVERE THYROID (TY) : -HYPERPLASIA, C-CELL,-SLIGHT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : >UNREMARKABLE 3595 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1834 ANIMAL NUMBER: C93095 SEX: FEMALE DATE OF DEATH: 04/17/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/17/00 16:05 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DEATH: 729 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: NOT ENTERED PROSECTOR: JODI THIEREN RECORDER: TONY HANFELD PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: MULTIPLE DARK BROWN AREAS, UP TO 2 MM IN DIAMETER SKIN (SK) : >NOTE:>SKIN WAS TAKEN FROM RIGHT SIDE DUE TO MASS ON LEFT MAMMARY, FEMALE (MF) : -MASS(ES); LEFT AXILLARY REGION: SINGLE MASS, 38 X 25 X 15 MM, RED, TAN, LOBULATED AND FIRM; ON SECTION, EXUDED A TAN FLUID; COLLECTED; REGIONAL LN (SEE AXILLARY); OVERLYING SKIN HAS A DARK RED AND DRY CRUSTED AREA, 35 X 25 MM; COLLECTED INTACT WITH MASS -MASS #2; LEFT INGUINAL EXTENDING INTO VENTRAL ABDOMINAL: SINGLE MASS, 65 X 50 X 31 MM, RED, DARK RED, TAN, LOBULATED AND FIRM: SOME SECTIONS EXUDE A RED FLUID; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED >NOTE:>MAMMARY WAS TAKEN FROM RIGHT SIDE DUE TO MASS ON LEFT UTERUS (UT) : -CYST(S); RIGHT HORN, WITHIN WALL: MULTIPLE CLEAR FLUID FILLED CYSTS, 2 MM IN DIAMETER MAMMARY, FEMALE (MF) : -M-CARCINOMA,-NEOPLASM PRESENT -CYST / GALACTOCELE,-PRESENT -B-FIBROADENOMA,-NEOPLASM PRESENT >NOTE:>MASS #1 IS THE CARCINOMA UTERUS (UT) : >UNREMARKABLE 3596 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1835 ANIMAL NUMBER: C93095 SEX: FEMALE DATE OF DEATH: 04/17/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/17/00 16:05 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DEATH: 729 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: NOT ENTERED PROSECTOR: JODI THIEREN RECORDER: TONY HANFELD PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY VAGINA (VA) : -SEMIFLUID MATERIAL; WITHIN LUMEN: LIGHT GREEN MUCOID MATERIAL LN, AXILLARY (AX) : -LARGE; LEFT: UP TO 12 X 10 X 3 MM (RIGHT NOT IDENTIFIED) (CAP 21) GENERAL COMMENT (GC) : -FLUID-THORACIC CAVITY; CLEAR FLUID VAGINA (VA) : -LUMEN, EXUDATE,-PRESENT -HYPERPLASIA, MUCINOUS,-MINIMAL CAUSE OF DEATH (CD) : -NEOPLASM, MAMMARY GLAND,-PRESENT LN, AXILLARY (AX) : -HYPERPLASIA, LYMPHOPLASMACYTIC,MODERATELY SEVERE 3597 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1836 ANIMAL NUMBER: C93095 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 04/17/00 STUDY DAY OF DEATH: 729 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 04/17/00 16:05 PROSECTOR: JODI THIEREN RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), LN, MEDIASTINAL (ML), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC(AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC(TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), LN, MESENTERIC (MS), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL(SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), HEMATO NEOPLASIA (HN) 3598 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1837 ANIMAL NUMBER: C93096 SEX: FEMALE DATE OF DEATH: 11/22/98 STUDY DAY OF DATE AND TIME OF NECROPSY: 11/22/98 8:04 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DEATH: 217 STUDY WEEK OF DEATH: 31 TERMINAL BODY WEIGHT: NOT ENTERED PROSECTOR: TODD ACKER RECORDER: TODD ACKER PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY GENERAL COMMENT (GC) : -MALOCCLUSION; BILATERAL, UPPER INCISORS -DISCHARGE-OCULAR; RIGHT: DARK RED AND DRY HISTOPATHOLOGY LIVER (LI) : -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL -DEGENERATION / NECROSIS, CENTRILOBULAR,-SLIGHT KIDNEY (KD) : -MINERALIZATION, TUBULAR,-MINIMAL -VACUOLATION, TUBULAR EPITHELIUM,MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MINIMAL -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SLIGHT LUNG (LU) : -CONGESTION,-PRESENT -MINERALIZATION, VASCULAR,-MINIMAL HEART (HT) : -MINERALIZATION, VASCULAR,-MINIMAL CAUSE OF DEATH (CD) : -UNDETERMINED MICROSCOPICALLY,-PRESENT 3599 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1838 ANIMAL NUMBER: C93096 SEX: FEMALE DATE OF DEATH: 11/22/98 STUDY DAY OF DATE AND TIME OF NECROPSY: 11/22/98 8:04 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DEATH: 217 STUDY WEEK OF DEATH: 31 TERMINAL BODY WEIGHT: NOT ENTERED PROSECTOR: TODD ACKER RECORDER: TODD ACKER PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 3600 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1839 ANIMAL NUMBER: C93097 SEX: FEMALE DATE OF DEATH: 02/28/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 02/28/00 8:33 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 680 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: 405.5 GRAMS PROSECTOR: DENNIS HOFFMAN RECORDER: TONE EVERTS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK 63; 40 X 35MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); AXILLARY REGION-LEFT; WEEK 69; 55 X 60MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); VENTRAL-CERVICAL; WEEK 78; 50 X 50MM; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; VENTRALTHORACIC-LEFT; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; BLACK SKIN; VENTRALTHORACIC-LEFT; VERIFIED AT NECROPSY: NOT APPLICABLE >DISCHARGE; OTHER; AXILLARY LEFT MASS BLEEDING; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-SLIGHT -NECROSIS, INDIVIDUAL HEPATOCYTE,MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-SLIGHT -HYPERTROPHY, CORTICAL CELL,-SLIGHT -HYPERPLASIA, CORTICAL,-MINIMAL -PIGMENT,-SLIGHT MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,MODERATELY SEVERE 3601 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1840 ANIMAL NUMBER: C93097 SEX: FEMALE DATE OF DEATH: 02/28/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 02/28/00 8:33 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 680 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: 405.5 GRAMS PROSECTOR: DENNIS HOFFMAN RECORDER: TONE EVERTS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 9 X 7 X 4 MM, RED, DARK RED, TAN, AND FIRM MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT CERVICAL REGION, EXTENDING INTO RIGHT AXILLARY REGION: SINGLE MASS, 55 X 50 X 22 MM, FIRM, LOBULATED, RED, AND TAN; ON SECTION SOME AREAS CONTAIN A TAN CREAMY SEMIFLUID; COLLECTED; REGIONAL LN (SEE AXILLARY) -MASS #2; LEFT INGUINAL REGION: SINGLE MASS, 47 X 36 X 23 MM, FIRM, LOBULATED, RED, AND TAN; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,-MODERATE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),SLIGHT THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -M-CARCINOMA,-NEOPLASM PRESENT -CYST / GALACTOCELE,-PRESENT -B-FIBROADENOMA,- 2 NEOPLASMS PRESENT -HYPERPLASIA, GLANDULAR,-SLIGHT >NOTE:>THE CARCINOMA IS MASS #3 3602 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1841 ANIMAL NUMBER: C93097 SEX: FEMALE DATE OF DEATH: 02/28/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 02/28/00 8:33 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 680 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: 405.5 GRAMS PROSECTOR: DENNIS HOFFMAN RECORDER: TONE EVERTS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS #3; LEFT AXILLARY REGION: SINGLE MASS, 62 X 56 X 50 MM, RED, DARK RED, TAN, FIRM, AND LOBULATED, ON SECTION, SOME AREAS CONTAIN A BROWN FLUID; COLLECTED; OVERLYING SKIN HAS A SINGLE DRY, RED, BROWN ULCERATED AREA, 30 X 45 MM, WHICH EXUDES A DARK RED FLUID; COLLECTED WITH MASS; REGIONAL LN (SEE AXILLARY) LN, AXILLARY (AX) : -LARGE; BILATERAL: LEFT IS 15 X 8 X 3 MM, RIGHT IS 9 X 5 X 2 MM (CAP 21) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN CERVIX (CV) : -HYPERPLASIA, MUCINOUS,-SLIGHT VAGINA (VA) : -LUMEN, EXUDATE,-PRESENT -HYPERPLASIA, MUCINOUS,-MODERATELY SEVERE CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT LN, AXILLARY (AX) : -HYPERPLASIA, LYMPHOPLASMACYTIC,MODERATELY SEVERE 3603 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1842 ANIMAL NUMBER: C93097 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/28/00 STUDY DAY OF DEATH: 680 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: 405.5 GRAMS DATE AND TIME OF NECROPSY: 02/28/00 8:33 PROSECTOR: DENNIS HOFFMAN RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUm /s KULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), HEMATO NEOPLASIA (HN) 3604 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1843 ANIMAL NUMBER: C93098 SEX: FEMALE DATE OF DEATH: 04/20/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/20/00 10:08 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 469.4 GRAMS PROSECTOR: CHANESTA ACKER RECORDER: TONE EVERTS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); INGUINAL REGION-LEFT; WEEK 100; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY OVARY (OV) : -CYST(S); RIGHT: SINGLE CLEAR FLUID FILLED CYST, 3 MM IN DIAMETER PITUITARY (PI) : -LARGE; 6 X 5 X 3 MM, APPEARS SWOLLEN HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SLIGHT -MINERALIZATION, PELVIC,-MODERATE -HYPERPLASIA, TRANSITIONAL CELL,-SLIGHT OVARY (OV) : -CYST,-PRESENT LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-MODERATE PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT -HYPERPLASIA,-MINIMAL MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT 3605 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1844 ANIMAL NUMBER: C93098 SEX: FEMALE DATE OF DEATH: 04/20/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/20/00 10:08 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 469.4 GRAMS PROSECTOR: CHANESTA ACKER RECORDER: TONE EVERTS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS(ES); LEFT INGUINAL REGION: SINGLE MASS, 10 X 9 X 6 MM, RED, TAN, AND FIRM; SAME ON CUT SURFACE (CAP 22) REGIONAL LN (INGUINAL) NOT IDENTIFIED -THICKENED; ALL REGIONS DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION -CYST(S); ALL REGIONS DIFFUSE: MULTIPLE WHITE CREAMY FLUID FILLED CYSTS, UP TO 4 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION UTERUS (UT) : -MASS(ES); RIGHT HORN WITHIN WALL: MULTIPLE DARK RED AND TAN PEDUNCULATED MASSES, EACH IS UP TO 5 X 4 X 3 MM; COLLECTED INTACT; REGIONAL LN (LUMBAR) NORMAL (CAP 23) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN MAMMARY, FEMALE (MF) : -HYPERPLASIA, GLANDULAR,-MINIMAL -M-FIBROSARCOMA,-NEOPLASM PRESENT UTERUS (UT) : -B-ADENOMA,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, LUMBAR/ILIAC (LM) : -HYPERPLASIA, LYMPHOPLASMACYTIC,-SLIGHT 3606 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1845 ANIMAL NUMBER: C93098 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 469.4 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 10:08 PROSECTOR: CHANESTA ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), PARATHYROID (PT), URINARY BLADDER (UB) 3607 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1846 ANIMAL NUMBER: C93099 SEX: FEMALE DATE OF DEATH: 02/19/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 02/19/00 7:39 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 671 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: 420.6 GRAMS PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); AXILLARY REGION-LEFT; WEEK 79; 30 X 20MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK 72; 20 X 20MM; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; HEAD TILT; LEFT; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >EXCRETION; NO FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >DISCHARGE; GENITAL; RED IN COLOR; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -MINERALIZATION,-MINIMAL LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -HYPERPLASIA, BILE DUCT,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, PELVIC,-SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,MODERATELY SEVERE -INFILTRATION, FAT, INTERSTITIAL,SLIGHT ADRENAL, CORTEX (AC) : -PIGMENT,-SLIGHT -ATROPHY, CORTICAL,-SLIGHT 3608 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1847 ANIMAL NUMBER: C93099 SEX: FEMALE DATE OF DEATH: 02/19/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 02/19/00 7:39 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 671 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: 420.6 GRAMS PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); SINGLE MASS, 14 X 11 X 6 MM, RED, DARK RED, TAN, SOFT, AND LOBULATED MAMMARY, FEMALE (MF) : -MASS(ES); LEFT INGUINAL REGION: SINGLE MASS, 25 X 25 X 16 MM, RED, TAN, AND FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED -MASS #2; LEFT AXILLARY REGION: SINGLE MASS, 30 X 20 X 15 MM, TAN AND SOFT; ON SECTION EXUDES A CLEAR FLUID; COLLECTED; REGIONAL LN (AXILLARY) NOT IDENTIFIED THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY) SLIGHT THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL -B-ADENOMA, FOLLICULAR CELL,-NEOPLASM PRESENT PARATHYROID (PT) : >SECTION EXAMINED; TISSUE NOT PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT -B-FIBROADENOMA,-NEOPLASM PRESENT -HYPERPLASIA, GLANDULAR,-SLIGHT -B-ADENOMA,-NEOPLASM PRESENT >NOTE:>THE ADENOMA IS MASS #2 UTERUS (UT) : -B-POLYP, ENDOMETRIAL, STROMAL,NEOPLASM PRESENT 3609 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1848 ANIMAL NUMBER: C93099 SEX: FEMALE DATE OF DEATH: 02/19/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 02/19/00 7:39 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 671 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: 420.6 GRAMS PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY UTERUS (UT) : -MASS(ES); WITHIN LUMEN OF RIGHT HORN: SINGLE MASS, 16 X 6 X 6 MM, DARK RED, SOFT, AND LOBULATED; SAME ON CUT SURFACE; COLLECTED INTACT WITH UTERUS; REGIONAL LN (LUMBAR) NORMAL (CAP 21) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -STAINS-PERINEUM/PERIANAL; AND DRY DARK RED UTERUS (UT) : -HYPERPLASIA, CYSTIC, ENDOMETRIAL,MODERATELY SEVERE VAGINA (VA) : -HYPERPLASIA, MUCINOUS,-SLIGHT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 3610 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1849 ANIMAL NUMBER: C93099 SEX: FEMALE DATE OF DEATH: 02/19/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 02/19/00 7:39 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 671 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: 420.6 GRAMS PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUm /s KULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), HEMATO NEOPLASIA (HN), LN, LUMBAR/ILIAC (LM) 3611 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1850 ANIMAL NUMBER: C93100 SEX: FEMALE DATE OF DEATH: 12/28/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 12/28/99 7:10 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DEATH: 618 STUDY WEEK OF DEATH: 89 TERMINAL BODY WEIGHT: NOT ENTERED PROSECTOR: GREGORY CODDINGTON RECORDER: DENNIS HOFFMAN PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-MODERATE -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-SLIGHT KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE PANCREAS (PA) : -INFILTRATION, FAT, INTERSTITIAL,SLIGHT ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MINIMAL -PIGMENT,-SLIGHT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATE HEART (HT) : -CARDIOMYOPATHY,-MINIMAL 3612 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1851 ANIMAL NUMBER: C93100 SEX: FEMALE DATE OF DEATH: 12/28/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 12/28/99 7:10 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DEATH: 618 STUDY WEEK OF DEATH: 89 TERMINAL BODY WEIGHT: NOT ENTERED PROSECTOR: GREGORY CODDINGTON RECORDER: DENNIS HOFFMAN PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); SINGLE MASS, 7 X 6 X 4 MM, DARK RED, TAN, GRAY AND SOFT LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL: DARK RED (CAP 21) PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT DUODENUM (DU) : >AUTOLYTIC ILEUM (IL) : >AUTOLYTIC CECUM (CE) : >AUTOLYTIC COLON (CO) : >AUTOLYTIC MAMMARY, FEMALE (MF) : -B-FIBROADENOMA,-NEOPLASM PRESENT UTERUS (UT) : -DILATATION,-MODERATE CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT LN, MANDIBULAR (MN) : -HYPERPLASIA, LYMPHOPLASMACYTIC,-SLIGHT 3613 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1852 ANIMAL NUMBER: C93100 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/28/99 STUDY DAY OF DEATH: 618 STUDY WEEK OF DEATH: 89 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/28/99 7:10 PROSECTOR: GREGORY CODDINGTON RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG), GENERAL COMMENT (GC) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), JEJUNUM (JE), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 3614 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1853 ANIMAL NUMBER: C93101 SEX: FEMALE DATE OF DEATH: 04/20/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/20/00 10:52 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 458.8 GRAMS PROSECTOR: TODD ACKER RECORDER: TONE EVERTS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY ADRENAL, CORTEX (AC) : -LARGE; RIGHT: 6 X 4 X 3 MM (LEFT IS 5 X 3 X 3 MM) GENERAL COMMENT (GC) -BLOOD FILM TAKEN HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-SLIGHT PANCREAS (PA) : -HYPERTROPHY / BASOPHILIA, ACINAR CELL,MINIMAL ADRENAL, CORTEX (AC) : -HYPERTROPHY, CORTICAL CELL,-MINIMAL -PIGMENT,-MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PARATHYROID (PT) : -HYPERPLASIA,-SLIGHT MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 3615 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1854 ANIMAL NUMBER: C93101 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 458.8 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 10:52 PROSECTOR: TODD ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: KIDNEY (KD), ADRENAL, MEDULLA (MA), LUNG (LU), URINARY BLADDER (UB) 3616 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1855 ANIMAL NUMBER: C93102 SEX: FEMALE DATE OF DEATH: 04/21/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/21/00 7:27 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 519.9 GRAMS PROSECTOR: STEVE VAN ADESTINE RECORDER: LAURIE J. SCHULLER PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -MOTTLED; ALL LOBES: RED AND TAN OVARY (OV) : -CYST(S); RIGHT: SINGLE CLEAR FLUID FILLED CYST, 4 MM IN DIAMETER HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-SLIGHT -HYPERPLASIA, BILE DUCT,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, PELVIC,-MINIMAL PANCREAS (PA) : -ATROPHY, ACINAR,-MINIMAL OVARY (OV) : -CYST,-PRESENT LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL -HYPERPLASIA, TYPE II PNEUMOCYTE,MINIMAL 3617 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1856 ANIMAL NUMBER: C93102 SEX: FEMALE DATE OF DEATH: 04/21/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/21/00 7:27 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 519.9 GRAMS PROSECTOR: STEVE VAN ADESTINE RECORDER: LAURIE J. SCHULLER PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 6 X 5 X 7 MM, RED, DARK RED, GRAY, AND SOFT MAMMARY, FEMALE (MF) : -THICKENED; ALL REGIONS, DIFFUSE: COLLECTED ON ROUTINE SECTION UTERUS (UT) : -CYST(S); RIGHT WITHIN WALL: SINGLE CLEAR FLUID FILLED CYST, 3 MM IN DIAMETER GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT -CYST,-PRESENT MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT -HYPERPLASIA, GLANDULAR,-SLIGHT UTERUS (UT) : -HYPERPLASIA, CYSTIC, ENDOMETRIAL,MINIMAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 3618 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1857 ANIMAL NUMBER: C93102 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 519.9 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 7:27 PROSECTOR: STEVE VAN ADESTINE RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: THYROID (TY), PARATHYROID (PT), URINARY BLADDER (UB) 3619 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1858 ANIMAL NUMBER: C93103 SEX: FEMALE DATE OF DEATH: 03/10/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 03/10/00 13:04 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 691 STUDY WEEK OF DEATH: 99 TERMINAL BODY WEIGHT: 391.1 GRAMS PROSECTOR: DAVID SCHUETTE RECORDER: TONE EVERTS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; SORE/SCAB; PAW-HINDLEFT; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; PAW-HINDRIGHT; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; MISSING; TEETH; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >EXCRETION; NO FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; BROWN HAIR COAT; VENTRAL-ABDOMINAL; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -DIFFUSELY LIGHT; ALL LOBES: TAN HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-SLIGHT -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-SEVERE -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-SLIGHT -FIBROSIS, PERIBILIARY,-SLIGHT -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, PELVIC,-MINIMAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-MODERATE ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT -PIGMENT,-SLIGHT 3620 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1859 ANIMAL NUMBER: C93103 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/10/00 STUDY DAY OF DEATH: 691 STUDY WEEK OF DEATH: 99 TERMINAL BODY WEIGHT: 391.1 GRAMS DATE AND TIME OF NECROPSY: 03/10/00 13:04 PROSECTOR: DAVID SCHUETTE RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY OVARY (OV) : -CYST(S); RIGHT: SINGLE CLEAR FLUID FILLED CYST, 2 MM IN DIAMETER BONE, FEMUR (FE) : >NOTE:>TRIMMING COMMENT: FEMUR NOT FOUND AT TIME OF TRIMMING MARROW, FEMUR (FM) : >NOTE:>TRIMMING COMMENT: FEMUR NOT FOUND AT TIME OF TRIMMING PITUITARY (PI) : -MASS(ES); 12 X 9 X 5 MM, DARK RED, TAN, AND SOFT STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA: SINGLE TAN ULCERATED AREA, 12 X 7 MM -THICKENED WALL; DIFFUSE MAMMARY, FEMALE (MF) : -THICKENED; ALL REGIONS DIFFUSE: TAN; COLLECTED ON ROUTINE SECTION OVARY (OV) : -CYST,-PRESENT BONE, FEMUR (FE) : >TISSUE MISSING MARROW, FEMUR (FM) : >TISSUE MISSING THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY) MODERATE HEART (HT) : -CARDIOMYOPATHY,-MODERATE THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, NONGL (SU) : -ACANTHOSIS / HYPERKERATOSIS,MODERATELY SEVERE -ULCERATION,-MODERATE -INFLAMMATION, CHRONIC-ACTIVE,-SEVERE -FIBROSIS,-MODERATELY SEVERE MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT -HYPERPLASIA, GLANDULAR,-SLIGHT 3621 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1860 ANIMAL NUMBER: C93103 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/10/00 STUDY DAY OF DEATH: 691 STUDY WEEK OF DEATH: 99 TERMINAL BODY WEIGHT: 391.1 GRAMS DATE AND TIME OF NECROPSY: 03/10/00 13:04 PROSECTOR: DAVID SCHUETTE RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY FOOT/FOOTPAD (FP) : -ABRASION(S)/ULCERATION(S); BILATERAL HINDFEET: SINGLE MOIST TAN ULCERATED AREA ON EACH, EACH IS 6 MM IN DIAMETER; BOTH ARE COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; UPPER RIGHT INCISOR -TEETH MISSING/BROKEN/WORN; UPPER LEFT INCISOR: MISSING -STAINS - SKIN/HAIRCOAT; VENTRAL ABDOMINAL: MOIST BROWN MATERIAL MAMMARY, FEMALE (MF) : -B-ADENOMA,-NEOPLASM PRESENT >NOTE:>NEOPLASM IS ON SLIDE #19 UTERUS (UT) : -HYPERPLASIA, CYSTIC, ENDOMETRIAL,MINIMAL VAGINA (VA) : -LUMEN, EXUDATE,-PRESENT -HYPERPLASIA, MUCINOUS,-MINIMAL CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT FOOT/FOOTPAD (FP) : -INFLAMMATION, CHRONIC-ACTIVE,-SEVERE -ULCERATION,-SLIGHT -ACANTHOSIS / HYPERKERATOSIS,-MODERATE 3622 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1861 ANIMAL NUMBER: C93103 SEX: FEMALE DATE OF DEATH: 03/10/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 03/10/00 13:04 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 691 STUDY WEEK OF DEATH: 99 TERMINAL BODY WEIGHT: 391.1 GRAMS PROSECTOR: DAVID SCHUETTE RECORDER: TONE EVERTS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), HEMATO NEOPLASIA (HN) 3623 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1862 ANIMAL NUMBER: C93104 SEX: FEMALE DATE OF DEATH: 11/23/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 11/23/99 15:45 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 583 STUDY WEEK OF DEATH: 84 TERMINAL BODY WEIGHT: 316.5 GRAMS PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK 58; 45 X 30MM; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; VENTRALTHORACIC-LEFT; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) HISTOPATHOLOGY BRAIN (BR) : -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED.-MINIMAL -NECROSIS, INDIVIDUAL HEPATOCYTE,MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, PELVIC,-MODERATELY SEVERE -HYPERPLASIA, TRANSITIONAL CELL,MODERATE SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MINIMAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MINIMAL -PIGMENT,-SLIGHT MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,-MODERATE 3624 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1863 ANIMAL NUMBER: C93104 SEX: FEMALE DATE OF DEATH: 11/23/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 11/23/99 15:45 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 583 STUDY WEEK OF DEATH: 84 TERMINAL BODY WEIGHT: 316.5 GRAMS PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 13 X 12 X 9 MM, DARK RED, TAN AND SOFT SKIN (SK) : -CRUSTED AREA(S); LEFT LATERAL THORACIC REGION: TWO TAN, RED AND DRY SLIGHTLY RAISED AREAS, ONE IS 7 X 6 X 3 MM, THE OTHER IS 8 X 6 X 5 MM (CAP 22) >NOTE:>SECTION TAKEN FROM RIGHT SIDE DUE TO MASS MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,-MODERATE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MINIMAL LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL HEART (HT) : -CARDIOMYOPATHY,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL SKIN (SK) : -ACANTHOSIS/HYPERKERATOSIS,-MODERATE -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE -ULCERATION,-MODERATE >NOTE:>SEE ALSO MAMMARY GLAND MAMMARY, FEMALE (MF) : -M-CARCINOMA,- 2 NEOPLASMS PRESENT -CYST / GALACTOCELE,-PRESENT -B-FIBROADENOMA,-NEOPLASM PRESENT -HYPERPLASIA, GLANDULAR,-SLIGHT 3625 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1864 ANIMAL NUMBER: C93104 SEX: FEMALE DATE OF DEATH: 11/23/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 11/23/99 15:45 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 583 STUDY WEEK OF DEATH: 84 TERMINAL BODY WEIGHT: 316.5 GRAMS PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS(ES); LEFT INGUINAL REGION: MULTIPLE MASSES, UP TO 30 X 30 X 15 MM, SOME ARE TAN AND SOFT; SAME ON CUT SURFACE; SOME ARE DARK RED, TAN AND SOFT; CUT SURFACE EXUDES A DARK RED SEMIFLUID; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED >NOTE:>SECTION TAKEN FROM RIGHT SIDE DUE TO MASS LN, MANDIBULAR (MN) : -LARGE; BILATERAL: EACH IS UP TO 10 X 7 X 3 MM (CAP 21) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN MAMMARY, FEMALE (MF) : >NOTE:>THE MASS CONTAINS BOTH CARCINOMA AND FIBROADENOMA THE SECOND MAMMARY CARCINOMA WAS IN THE ULCERATED SKIN SECTION CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT LN, MANDIBULAR (MN) : -HYPERPLASIA, LYMPHOPLASMACYTIC,-SEVERE 3626 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1865 ANIMAL NUMBER: C93104 SEX: FEMALE DATE OF DEATH: 11/23/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 11/23/99 15:45 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 583 STUDY WEEK OF DEATH: 84 TERMINAL BODY WEIGHT: 316.5 GRAMS PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 3627 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1866 ANIMAL NUMBER: C93105 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/28/99 STUDY DAY OF DEATH: 618 STUDY WEEK OF DEATH: 89 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/28/99 7:26 PROSECTOR: GREGORY CODDINGTON RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; SWOLLEN; LIMB-HIND-RIGHT; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) ADRENAL, CORTEX (AC) : -LARGE; LEFT: 10 MM IN DIAMETER IS 5 MM IN DIAMETER) LN, MESENTERIC (MS) : -DIFFUSELY DARK; DARK BROWN (RIGHT HISTOPATHOLOGY BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, PELVIC,-MINIMAL -HYPERPLASIA, TRANSITIONAL CELL,-SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MINIMAL ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC / HEMORRHAGIC SEVERE LN, MESENTERIC (MS) : >UNREMARKABLE 3628 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1867 ANIMAL NUMBER: C93105 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/28/99 STUDY DAY OF DEATH: 618 STUDY WEEK OF DEATH: 89 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/28/99 7:26 PROSECTOR: GREGORY CODDINGTON RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); SINGLE MASS, 13 X 9 X 9 MM, DARK RED, GREY, TAN AND SOFT MAMMARY, FEMALE (MF) : -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM (COLLECTED ON ROUTINE SECTION) FOOT/FOOTPAD (FP) : -CRUSTED AREA(S); RIGHT HINDFOOT: SINGLE TAN AND DRY CRUSTED AREA, 4 X 3 MM; SURROUNDING TISSUE APPEARS SWOLLEN (COLLECTED) THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),SLIGHT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT JEJUNUM (JE) : >AUTOLYTIC ILEUM (IL) : >AUTOLYTIC CECUM (CE) : >AUTOLYTIC MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT -HYPERPLASIA, GLANDULAR,-MODERATE UTERUS (UT) : -DILATATION,-SLIGHT VAGINA (VA) : -HYPERPLASIA, MUCINOUS,-MINIMAL CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT FOOT/FOOTPAD (FP) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE -ULCERATION,-SLIGHT -ACANTHOSIS / HYPERKERATOSIS,-MODERATE 3629 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1868 ANIMAL NUMBER: C93105 SEX: FEMALE DATE OF DEATH: 12/28/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 12/28/99 7:26 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DEATH: 618 STUDY WEEK OF DEATH: 89 TERMINAL BODY WEIGHT: NOT ENTERED PROSECTOR: GREGORY CODDINGTON RECORDER: DENNIS HOFFMAN PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -MALOCCLUSION; BILATERAL UPPER INCISORS THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), COLON (CO), RECTUM (RE), SKIN URINARY BLADDER (UB), CERVIX (CV), HEMATO NEOPLASIA (HN) (SK), 3630 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1869 ANIMAL NUMBER: C93106 SEX: FEMALE DATE OF DEATH: 02/09/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 02/09/00 11:36 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 661 STUDY WEEK OF DEATH: 95 TERMINAL BODY WEIGHT: 410.2 GRAMS PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MISSING; TAIL-DISTAL; VERIFIED AT NECROPSY: OBSERVED >EYES; RED DISCHARGE; EYES; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -LIGHT FOCUS(I)/AREA(S); AT JUNCTION OF MEDIAN LOBES: SINGLE TAN AREA, 4 MM IN DIAMETER (CAP 21) -RED FOCUS(I)/AREA(S); NEAR JUNCTION OF MEDIAN LOBES: MULTIPLE DARK RED AREAS, UP TO 4 MM IN DIAMETER (CAP 21) -DARK FOCUS(I)/AREA(S); DIAPHRAGMATIC SURFACE OF LEFT LATERAL LOBE: SINGLE GRAY, LINEAR AREA, 4 X 2 MM; COLLECTED ON ROUTINE SECTION HISTOPATHOLOGY BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-MODERATE -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL -DEGENERATION, CYSTIC,-MINIMAL -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL -VACUOLATION, HEPATOCELLULAR,-MINIMAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATELY SEVERE PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-SLIGHT ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT -PIGMENT,-SLIGHT 3631 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1870 ANIMAL NUMBER: C93106 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/09/00 STUDY DAY OF DEATH: 661 STUDY WEEK OF DEATH: 95 TERMINAL BODY WEIGHT: 410.2 GRAMS DATE AND TIME OF NECROPSY: 02/09/00 11:36 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 10 X 8 X 7 MM, DARK RED, GRAY AND SOFT VAGINA (VA) : -SEMIFLUID MATERIAL; WITHIN LUMEN: SMALL AMOUNT OF LIGHT GREEN SEMIFLUID GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -TAIL - BENT/MISSING; TIP: MISSING; REMAINING PORTION COLLECTED -DISCHARGE-OCULAR; BILATERAL: DARK RED AND DRY THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATE HEART (HT) : -CARDIOMYOPATHY,-SLIGHT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -HYPERPLASIA, GLANDULAR,-MODERATE VAGINA (VA) : -HYPERPLASIA, MUCINOUS,-MODERATE CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 3632 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1871 ANIMAL NUMBER: C93106 SEX: FEMALE DATE OF DEATH: 02/09/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 02/09/00 11:36 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 661 STUDY WEEK OF DEATH: 95 TERMINAL BODY WEIGHT: 410.2 GRAMS PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUm /s KULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), HEMATO NEOPLASIA (HN) (SN), 3633 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1872 ANIMAL NUMBER: C93107 SEX: FEMALE DATE OF DEATH: 04/19/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/19/00 13:20 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 441.4 GRAMS PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; MISSING; TEETH; VERIFIED AT NECROPSY: NOT OBSERVED >MASS(ES); VENTRAL-CERVICAL-RIGHT; WEEK 81; 70 X 80MM; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY PITUITARY (PI) : -RED FOCUS(I)/AREA(S); LEFT ANTERIOR ASPECT: SINGLE DARK RED FOCUS, 2 MM IN DIAMETER MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT VENTRAL CERVICAL REGION: SINGLE MASS, 80 X 60 X 40 MM, TAN, RED, DARK RED AND SOFT; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (MANDIBULAR) NORMAL; COLLECTED HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL -NECROSIS, COAGULATIVE,-MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PITUITARY (PI) : -HYPERPLASIA,-MODERATELY SEVERE MAMMARY, FEMALE (MF) : -B-FIBROADENOMA,-NEOPLASM PRESENT -HYPERPLASIA, GLANDULAR,-MODERATE CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MANDIBULAR (MN) : -HYPERPLASIA, LYMPHOPLASMACYTIC,MODERATE 3634 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1873 ANIMAL NUMBER: C93107 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 441.4 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 13:20 PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -MALOCCLUSION; RIGHT UPPER INCISOR -TEETH MISSING/BROKEN/WORN; LEFT UPPER INCISOR, BROKEN AT BASE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: KIDNEY (KD), PANCREAS (PA), LUNG (LU), PARATHYROID (PT), URINARY BLADDER (UB) 3635 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1874 ANIMAL NUMBER: C93108 SEX: FEMALE DATE OF DEATH: 04/18/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/18/99 14:55 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DEATH: 364 STUDY WEEK OF DEATH: 52 TERMINAL BODY WEIGHT: NOT ENTERED PROSECTOR: TODD ACKER RECORDER: TODD ACKER PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY ADRENAL, CORTEX (AC) : -MASS(ES); INVOLVING ENTIRE RIGHT ADRENAL, SURROUNDING RIGHT KIDNEY: SINGLE MASS, 20 MM IN DIAMETER, DARK RED AND SOFT; CUT SURFACE EXUDES A DARK RED FLUID; COLLECTED; REGIONAL LN (RENAL) NOT IDENTIFIED HISTOPATHOLOGY KIDNEY (KD) : -MINERALIZATION, TUBULAR,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : -HEMORRHAGE,-PRESENT ADRENAL, MEDULLA (MA) : -UNILATERALLY EXAMINED,-PRESENT MARROW, FEMUR (FM) : -HYPOCELLULAR, MARROW,-SLIGHT THYMUS (TH) : -HEMORRHAGE,-PRESENT DUODENUM (DU) : >AUTOLYTIC JEJUNUM (JE) : >AUTOLYTIC ILEUM (IL) : >AUTOLYTIC CECUM (CE) : >AUTOLYTIC 3636 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1875 ANIMAL NUMBER: C93108 SEX: FEMALE DATE OF DEATH: 04/18/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/18/99 14:55 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DEATH: 364 STUDY WEEK OF DEATH: 52 TERMINAL BODY WEIGHT: NOT ENTERED PROSECTOR: TODD ACKER RECORDER: TODD ACKER PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY UTERUS (UT) : -DILATATION,-MODERATE CAUSE OF DEATH (CD) : -HEMORRHAGE,-PRESENT THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG), GENERAL COMMENT (GC) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LIVER (LI), PANCREAS (PA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 3637 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1876 ANIMAL NUMBER: C93109 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 259.1 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 9:38 PROSECTOR: JODI THIEREN RECORDER: KIMBERLY DURLAND POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) LF ADRENAL (AD0) RT ADRENAL (AD1) BRAIN (BR) LF KIDNEY (KD0) RT KIDNEY (KD1) LIVER (LI) LUNG (LU) LF OVARY (OV0) RT OVARY (OV1) SPLEEN (SP) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) UTERUS (UT) .0478 .0379 2.0285 1.1768 1.2596 10.0793 1.3962 .0846 .1074 .5017 ..00100817 .8250 .0184 % .0146 % .7829 % .4542 % .4861 % 3.8901 % .5389 % .0327 % .0415 % .1936 % .0034 % .0039 % .3184 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0236 .0187 1.0000 .5801 .6210 4.9688 .6883 .0417 .0529 .2473 .0043 .0050 .4067 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-MINIMAL KIDNEY (KD) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT 3638 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1877 ANIMAL NUMBER: C93109 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 259.1 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 9:38 PROSECTOR: JODI THIEREN RECORDER: KIMBERLY DURLAND POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV) 3639 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1878 ANIMAL NUMBER: C93110 SEX: FEMALE DATE OF DEATH: 01/09/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 01/09/00 9:45 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 630 STUDY WEEK OF DEATH: 90 TERMINAL BODY WEIGHT: 330.6 GRAMS PROSECTOR: DONIA MEYER RECORDER: DONIA MEYER PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); AXILLARY REGION-RIGHT; WEEK 90; 20 X 20MM; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; ROUGH HAIR COAT; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >EYES; RED DISCHARGE; EYE-LEFT; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; NONFORMED FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -MOTTLED; ALL LOBES: DARK RED AND TAN KIDNEY (KD) : -GRANULAR MATERIAL; BILATERAL, WITHIN PELVIS: MULTIPLE TAN GRANULES, 1 MM IN DIAMETER -ROUGH SURFACE, DIFFUSE; BILATERAL -MOTTLED; BILATERAL: DARK RED AND TAN HISTOPATHOLOGY EYE (EY) : -DYSTROPHY, CORNEAL IN EARLY STAGES, CONSISTS PRIMARILY OF FOCAL MINERALIZATION,-MINIMAL -INFLAMMATION, ACUTE,-SLIGHT BRAIN (BR) : >UNREMARKABLE LIVER (LI) : -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-SEVERE -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SEVERE -MINERALIZATION, PELVIC,-SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-SLIGHT 3640 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1879 ANIMAL NUMBER: C93110 SEX: FEMALE DATE OF DEATH: 01/09/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 01/09/00 9:45 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 630 STUDY WEEK OF DEATH: 90 TERMINAL BODY WEIGHT: 330.6 GRAMS PROSECTOR: DONIA MEYER RECORDER: DONIA MEYER PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 7 X 5 X 4 MM, DARK RED, TAN AND SOFT STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: MULTIPLE DARK BROWN FOCI, UP TO 2 MM IN DIAMETER MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT AXILLARY REGION: SINGLE MASS, 30 X 20 X 10 MM, TAN AND FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (SEE AXILLARY) ADRENAL, CORTEX (AC) : -HYPERTROPHY, CORTICAL CELL,-MINIMAL -DEGENERATION, CYSTIC / HEMORRHAGIC,SLIGHT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATELY SEVERE HEART (HT) : -CARDIOMYOPATHY,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT SALIV GL, MANDIB (SG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL STOMACH, GL (ST) : >UNREMARKABLE DUODENUM (DU) : -INFLAMMATION, VASCULAR,-SLIGHT SKIN (SK) : -B-LIPOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT >NOTE:>SEE SKIN FOR DIAGNOSIS 3641 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1880 ANIMAL NUMBER: C93110 SEX: FEMALE DATE OF DEATH: 01/09/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 01/09/00 9:45 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 630 STUDY WEEK OF DEATH: 90 TERMINAL BODY WEIGHT: 330.6 GRAMS PROSECTOR: DONIA MEYER RECORDER: DONIA MEYER PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY VAGINA (VA) : -SEMIFLUID MATERIAL; WITHIN VAGINA: LARGE AMOUNT OF GREEN SEMIFLUID LN, AXILLARY (AX) : -MOTTLED; BILATERAL: DARK RED AND TAN (CAP 21) GENERAL COMMENT (GC) -BLOOD FILM TAKEN -DISCHARGE-OCULAR; MATERIAL -ANIMAL THIN : BILATERAL: DRY RED VAGINA (VA) : -LUMEN, EXUDATE,-PRESENT CAUSE OF DEATH (CD) : -NEPHROPATHY,-PRESENT LN, AXILLARY (AX) : -HYPERPLASIA, LYMPHOPLASMACYTIC,MINIMAL -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL 3642 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1881 ANIMAL NUMBER: C93110 SEX: FEMALE DATE OF DEATH: 01/09/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 01/09/00 9:45 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 630 STUDY WEEK OF DEATH: 90 TERMINAL BODY WEIGHT: 330.6 GRAMS PROSECTOR: DONIA MEYER RECORDER: DONIA MEYER PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), LN, MEDIASTINAL (ML), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), STOMACH, NONGL (SU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), HEMATO NEOPLASIA (HN) 3643 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1882 ANIMAL NUMBER: C93111 SEX: FEMALE DATE OF DEATH: 07/21/98 STUDY DAY OF DATE AND TIME OF NECROPSY: 07/21/98 11:01 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 287.5 GRAMS PROSECTOR: STEVE VAN ADESTINE RECORDER: KIMBERLY DURLAND PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) LF ADRENAL (AD0) RT ADRENAL (AD1) BRAIN (BR) LF KIDNEY (KD0) RT KIDNEY (KD1) LIVER (LI) LUNG (LU) LF OVARY (OV0) RT OVARY (OV1) SPLEEN (SP) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) UTERUS (UT) .0325 .0326 2.2661 1.1506 1.1943 10.8555 1.4316 .0693 .0788 .4755 .0096 .0113 .4887 .0113 % .0113 % .7882 % .4002 % .4154 % 3.7758 % .4979 % .0241 % .0274 % .1654 % .0033 % .0039 % .1700 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0143 .0144 1.0000 .5077 .5270 4.7904 .6317 .0306 .0348 .2098 .0042 .0050 .2157 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-MINIMAL -NECROSIS, INDIVIDUAL HEPATOCYTE,MINIMAL -VACUOLATION, HEPATOCELLULAR, MIDZONAL / CENTRILOBULAR,-SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-MINIMAL 3644 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1883 ANIMAL NUMBER: C93111 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 287.5 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 11:01 PROSECTOR: STEVE VAN ADESTINE RECORDER: KIMBERLY DURLAND POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV) 3645 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1884 ANIMAL NUMBER: C93112 SEX: FEMALE DATE OF DEATH: 04/20/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/20/99 9:15 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 341.7 GRAMS PROSECTOR: TODD ACKER RECORDER: TONY HANFELD PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) LF ADRENAL (AD0) RT ADRENAL (AD1) BRAIN (BR) LF KIDNEY (KD0) RT KIDNEY (KD1) LIVER (LI) LUNG (LU) LF OVARY (OV0) RT OVARY (OV1) SPLEEN (SP) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) UTERUS (UT) .0342 .0328 2.2296 1.5592 1.6358 16.7616 1.5802 .0394 .0495 .4772 .0095 .0089 .8851 .0100 % .0096 % .6525 % .4563 % .4787 % 4.9054 % .4625 % .0115 % .0145 % .1397 % .0028 % .0026 % .2590 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0153 .0147 1.0000 .6993 .7337 7.5178 .7087 .0177 .0222 .2140 .0043 .0040 .3970 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED LIVER (LI) : -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-MODERATE -HYPERPLASIA, BILE DUCT,-MINIMAL -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL -CYST, BILIARY,-PRESENT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SLIGHT -MINERALIZATION, TUBULAR,-MINIMAL 3646 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1885 ANIMAL NUMBER: C93112 SEX: FEMALE DATE OF DEATH: 04/20/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/20/99 9:15 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 341.7 GRAMS PROSECTOR: TODD ACKER RECORDER: TONY HANFELD PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -RAISED AREA(S); MID ASPECT: SINGLE RED AREA, 2 MM IN DIAMETER GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL -ATROPHY, ACINAR,-MINIMAL ADRENAL, CORTEX (AC) : -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL -HYPERTROPHY, CORTICAL CELL,-MINIMAL MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,-MINIMAL MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,-SLIGHT THYMUS (TH) : -CYST,-PRESENT LUNG (LU) : -HEMORRHAGE,-PRESENT -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT -HYPERPLASIA,-MINIMAL MAMMARY, FEMALE (MF) : -SECRETORY MATERIAL,-PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 3647 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1886 ANIMAL NUMBER: C93112 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 341.7 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 9:15 PROSECTOR: TODD ACKER RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 3648 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1887 ANIMAL NUMBER: C93113 SEX: FEMALE DATE OF DEATH: 04/19/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/19/00 10:50 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 544.8 GRAMS PROSECTOR: JERALD W. JOHNSON RECORDER: TERRI L. WILLIAMS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK 103; 20 X 20MM; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE DARK RED AREAS, UP TO 6 MM IN DIAMETER; COLLECTED ON ROUTINE SECTIONS HISTOPATHOLOGY BRAIN (BR) : ^UNREMARKABLE LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-MODERATE -GRANULAR CYTOPLASM, EOSINOPHILIC, CENTRILOBULAR,-SLIGHT -PIGMENT, HEPATOCELLULAR, CENTRILOBULAR,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MODERATELY SEVERE -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL LIVER-OIL RED O (LI0) : -VACUOLES, OIL RED O-POSITIVE, HEPATOCELLULAR,-MODERATE KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, PELVIC,-SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE 3649 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1888 ANIMAL NUMBER: C93113 SEX: FEMALE DATE OF DEATH: 04/19/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/19/00 10:50 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 544.8 GRAMS PROSECTOR: JERALD W. JOHNSON RECORDER: TERRI L. WILLIAMS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY ADRENAL, CORTEX (AC) : -MOTTLED; BILATERAL: TAN AND DARK BROWN OVARY (OV) : -CYST(S); LEFT: SINGLE CLEAR FLUID FILLED CYST, 5 MM IN DIAMETER PITUITARY (PI) : -LARGE; 7 X 7 X 5 MM; APPEARS SWOLLEN SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE PANCREAS (PA) : -INFILTRATION, FAT, INTERSTITIAL,MINIMAL ADRENAL, CORTEX (AC) : -HYPERTROPHY, CORTICAL CELL,-MODERATELY SEVERE -DEGENERATION, CYSTIC / HEMORRHAGIC,SLIGHT -PIGMENT,-SLIGHT OVARY (OV) : -CYST,-PRESENT -HYPERPLASIA, GRANULOSA CELL,MODERATELY SEVERE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MINIMAL LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL HEART (HT) : -CARDIOMYOPATHY,-MINIMAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT 3650 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1889 ANIMAL NUMBER: C93113 SEX: FEMALE DATE OF DEATH: 04/19/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/19/00 10:50 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 544.8 GRAMS PROSECTOR: JERALD W. JOHNSON RECORDER: TERRI L. WILLIAMS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION; POSSIBLY CONSISTENT WITH MASS (VENTRAL ABDOMINAL LEFT) NOTED IN CLINICAL OBSERVATIONS -CYST(S); LEFT INGUINAL REGION: SINGLE TAN FLUID FILLED CYST, 5 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN PITUITARY (PI) : -HYPERPLASIA,-SLIGHT -CYST,-PRESENT HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-SLIGHT MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT -B-FIBROADENOMA,-NEOPLASM PRESENT -HYPERPLASIA, GLANDULAR,-SLIGHT URINARY BLADDER (UB) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL UTERUS (UT) : -HYPERPLASIA, CYSTIC, ENDOMETRIAL,SLIGHT CERVIX (CV) : -HYPERPLASIA, MUCINOUS,-MINIMAL VAGINA (VA) : -HYPERPLASIA, MUCINOUS,-MODERATELY SEVERE CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 3651 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1890 ANIMAL NUMBER: C93113 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 544.8 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 10:50 PROSECTOR: JERALD W. JOHNSON RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -ANIMAL OBESE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), HEMATO NEOPLASIA (HN) 3652 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1891 ANIMAL NUMBER: C93114 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 205.4 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 9:20 PROSECTOR: LYNN HORNER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN STATUS LF ADRENAL (AD0) RT ADRENAL (AD1) BRAIN (BR) LF KIDNEY (KD0) RT KIDNEY (KD1) LIVER (LI) LUNG (LU) LF OVARY (OV0) RT OVARY (OV1) SPLEEN (SP) LF THYROID/PARA RT THYROID/PARA UTERUS (UT) (TT0) (TT1) -------- 7.8332 -------- -------- -------- -------- 3.8144 % -------- -------- -------- -------- --------------- -------- -------- NOT TAKEN WEIGHT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS NOTTAK NOTTAK NOTTAK NOTTAK NOTTAK NOTTAK NOTTAK NOTTAK 3653 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1892 ANIMAL NUMBER: C93114 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 205.4 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 9:20 PROSECTOR: LYNN HORNER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) 3654 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1893 ANIMAL NUMBER: C93115 SEX: FEMALE DATE OF DEATH: 04/20/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/20/00 10:41 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 470.4 GRAMS PROSECTOR: CHANESTA ACKER RECORDER: TONE EVERTS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -LIGHT FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE TAN AREAS, UP TO 3 MM IN DIAMETER; COLLECTED ON ROUTINE SECTIONS HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-SLIGHT -GRANULAR CYTOPLASM, EOSINOPHILIC, CENTRILOBULAR,-MINIMAL -PIGMENT, HEPATOCELLULAR, CENTRILOBULAR,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MODERATE -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, PELVIC,-MINIMAL -MINERALIZATION, TUBULAR,-MINIMAL 3655 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1894 ANIMAL NUMBER: C93115 SEX: FEMALE DATE OF DEATH: 04/20/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/20/00 10:41 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 470.4 GRAMS PROSECTOR: CHANESTA ACKER RECORDER: TONE EVERTS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY ADRENAL, CORTEX (AC) : -LARGE; BILATERAL: LEFT IS 7 X 6 X 5 MM, RIGHT IS 5 X 5 X 4 MM OVARY (OV) : -LARGE; LEFT: 10 X 6 X 4 MM (RIGHT IS 6 X 5 X 3 MM) SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SLIGHT ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATE -HYPERTROPHY, CORTICAL CELL,-MINIMAL -DEGENERATION, CYSTIC / HEMORRHAGIC,MINIMAL -PIGMENT,-SLIGHT ADRENAL, MEDULLA (MA) : -HYPERPLASIA, MEDULLARY,-MINIMAL -B-PHEOCHROMOCYTOMA,-NEOPLASM PRESENT OVARY (OV) : -HYPERPLASIA, GRANULOSA CELL,MODERATELY SEVERE MARROW, FEMUR (FM) : -HYPOCELLULAR, MARROW,-SLIGHT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY) SLIGHT LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL HEART (HT) : -CARDIOMYOPATHY,-SLIGHT PARATHYROID (PT) : -B-ADENOMA,-NEOPLASM PRESENT 3656 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1895 ANIMAL NUMBER: C93115 SEX: FEMALE DATE OF DEATH: 04/20/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/20/00 10:41 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 470.4 GRAMS PROSECTOR: CHANESTA ACKER RECORDER: TONE EVERTS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 12 X 9 X 5 MM, DARK RED, TAN, AND SOFT MAMMARY, FEMALE (MF) : -THICKENED; ALL REGIONS DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT -HYPERPLASIA, GLANDULAR,-SLIGHT VAGINA (VA) : -HYPERPLASIA, MUCINOUS,-SLIGHT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 3657 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1896 ANIMAL NUMBER: C93115 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 470.4 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 10:41 PROSECTOR: CHANESTA ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), HEMATO NEOPLASIA (HN) 3658 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1897 ANIMAL NUMBER: C93116 SEX: FEMALE DATE OF DEATH: 04/20/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/20/99 11:22 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 288.9 GRAMS PROSECTOR: NANCY DIEDRICH RECORDER: TONY HANFELD PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) LF ADRENAL (AD0) RT ADRENAL (AD1) BRAIN (BR) LF KIDNEY (KD0) RT KIDNEY (KD1) LIVER (LI) LUNG (LU) LF OVARY (OV0) RT OVARY (OV1) SPLEEN (SP) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) UTERUS (UT) .0353 .0364 2.1339 1.4382 1.4407 13.8981 1.4611 .0612 .0620 .4803 .0085 .0104 1.2623 .0122 % .0126 % .7386 % .4978 % .4987 % 4.8107 % .5057 % .0212 % .0215 % .1663 % .0029 % .0036 % .4369 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0165 .0171 1.0000 .6740 .6751 6.5130 .6847 .0287 .0291 .2251 .0040 .0049 .5915 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-MODERATE -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL -PIGMENT, TUBULAR,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE 3659 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1898 ANIMAL NUMBER: C93116 SEX: FEMALE DATE OF DEATH: 04/20/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/20/99 11:22 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 288.9 GRAMS PROSECTOR: NANCY DIEDRICH RECORDER: TONY HANFELD PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY URINARY BLADDER (UB) : >NOTE:>TRIMMING COMMENT: URINARY BLADDER NOT FOUND AT TIME OF TRIMMING VAGINA (VA) : >NOTE:>TRIMMING COMMENT: VAGINA NOT FOUND AT TIME OF TRIMMING GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC / HEMORRHAGIC,SLIGHT LUNG (LU) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL MAMMARY, FEMALE (MF) : -SECRETORY MATERIAL,-PRESENT URINARY BLADDER (UB) : >TISSUE MISSING VAGINA (VA) : >TISSUE MISSING CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 3660 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1899 ANIMAL NUMBER: C93116 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 288.9 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 11:22 PROSECTOR: NANCY DIEDRICH RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), UTERUS (UT), CERVIX (CV), HEMATO NEOPLASIA (HN) 3661 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1900 ANIMAL NUMBER: C93117 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 390.9 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 11:04 PROSECTOR: TODD ACKER RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) LF ADRENAL (AD0) RT ADRENAL (AD1) BRAIN (BR) LF KIDNEY (KD0) RT KIDNEY (KD1) LIVER (LI) LUNG (LU) LF OVARY (OV0) RT OVARY (OV1) SPLEEN (SP) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) UTERUS (UT) .0325 .0369 2.2739 1.5504 1.4994 16.3241 1.6859 .0392 .0484 .7833 .0150 .0061 1.0597 .0083 % .0094 % .5817 % .3966 % .3836 % 4.1760 % .4313 % .0100 % .0124 % .2004 % .0038 % .0016 % .2711 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0143 .0162 1.0000 .6818 .6594 7.1789 .7414 .0172 .0213 .3445 .0066 .0027 .4660 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-MODERATE -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-MINIMAL KIDNEY (KD) : -MINERALIZATION, TUBULAR,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT 3662 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1901 ANIMAL NUMBER: C93117 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 390.9 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 11:04 PROSECTOR: TODD ACKER RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -LARGE; 6 X 5 X 3 MM; APPEARS SWOLLEN GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN ADRENAL, CORTEX (AC) : -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL MARROW, FEMUR (FM) : -HYPOCELLULAR, MARROW,-SLIGHT LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,SLIGHT PITUITARY (PI) : -HYPERPLASIA,-MODERATELY SEVERE -ANGIECTASIS,-PRESENT MAMMARY, FEMALE (MF) : -HYPERPLASIA, GLANDULAR,-MINIMAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 3663 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1902 ANIMAL NUMBER: C93117 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 390.9 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 11:04 PROSECTOR: TODD ACKER RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 3664 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1903 ANIMAL NUMBER: C93118 SEX: FEMALE DATE OF DEATH: 01/24/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 01/24/00 13:49 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 645 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: 387.8 GRAMS PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK 49; 90 X 90MM; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: NOT OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; MISSING; TEETH; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; VENTRALABDOMINAL-LEFT; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) SPLEEN (SP) : -LARGE; 60 X 15 X 10 MM HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,MODERATE -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-SLIGHT -GRANULAR CYTOPLASM, EOSINOPHILIC, CENTRILOBULAR,-SLIGHT -PIGMENT, HEPATOCELLULAR, CENTRILOBULAR.-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-SLIGHT -DEGENERATION, CYSTIC,-MINIMAL -INFLAMMATION, SUBACUTE,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, PELVIC,-MINIMAL -MINERALIZATION, TUBULAR,-MINIMAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATELY SEVERE 3665 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1904 ANIMAL NUMBER: C93118 SEX: FEMALE DATE OF DEATH: 01/24/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 01/24/00 13:49 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 645 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: 387.8 GRAMS PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY LUNG (LU) : -LIGHT FOCUS(I)/AREA(S); TRIMMING COMMENT: ALL LOBES: MULTIPLE TAN AREAS, UP TO 4 X 3 MM PITUITARY (PI) : -MASS(ES); 11 X 6 X 4 MM, RED, TAN AND SOFT PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-SLIGHT ADRENAL, CORTEX (AC) : -HYPERTROPHY, CORTICAL CELL,-MODERATELY SEVERE -HYPERPLASIA, CORTICAL,-MINIMAL ADRENAL, MEDULLA (MA) : -B-PHEOCHROMOCYTOMA,-NEOPLASM PRESENT MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,-SEVERE MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,-SEVERE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),SLIGHT LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,SLIGHT HEART (HT) : -CARDIOMYOPATHY,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -M-CARCINOMA,-NEOPLASM PRESENT 3666 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1905 ANIMAL NUMBER: C93118 SEX: FEMALE DATE OF DEATH: 01/24/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 01/24/00 13:49 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 645 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: 387.8 GRAMS PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT INGUINAL REGION EXTENDING LATERALLY TO LEFT INGUINAL REGION: MULTIPLE MASSES, UP TO 60 X 30 X 25 MM, ALL ARE RED, TAN, GREEN, LOBULATED AND FIRM; SAME ON ALL CUT SURFACES, ALL COLLECTED INTACT; OVERLYING SKIN HAS A SINGLE MOIST RED ULCERATED AREA, 10 MM IN DIAMETER; COLLECTED INTACT WITH MASSES; REGIONAL LN (INGUINAL) NOT IDENTIFIED LN, MANDIBULAR (MN) : -LARGE; BILATERAL: EACH IS 9 X 6 X 3 MM; COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -TEETH MISSING/BROKEN/WORN; MISSING, BILATERAL UPPER INCISORS -ANIMAL THIN CAUSE OF DEATH (CD) : -NEOPLASM, MAMMARY GLAND,-PRESENT LN, MANDIBULAR (MN) : -HYPERPLASIA, LYMPHOPLASMACYTIC,MODERATE 3667 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1906 ANIMAL NUMBER: C93118 SEX: FEMALE DATE OF DEATH: 01/24/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 01/24/00 13:49 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 645 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: 387.8 GRAMS PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), TAIL (TI), LN, LUMBAr /i LIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 3668 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1907 ANIMAL NUMBER: C93119 SEX: FEMALE DATE OF DEATH: 01/17/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 01/17/00 15:17 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 638 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: 260.4 GRAMS PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; SHOULDERRIGHT; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; ROUGH HAIR COAT; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; SORE/SCAB; DORSAL; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) ADRENAL, CORTEX (AC) : -LARGE; RIGHT: 7 MM IN DIAMETER (LEFT IS 4 MM IN DIAMETER) HISTOPATHOLOGY EYE (EY) : -INFLAMMATION, ACUTE,-SLIGHT BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-MODERATE -GRANULAR CYTOPLASM, EOSINOPHILIC, CENTRILOBULAR,-MODERATE -PIGMENT, HEPATOCELLULAR, CENTRILOBULAR,-SLIGHT -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : -HYPERTROPHY, CORTICAL CELL,-MODERATE -DEGENERATION, CYSTIC / HEMORRHAGIC,SEVERE -PIGMENT,-SLIGHT MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,-SLIGHT 3669 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1908 ANIMAL NUMBER: C93119 SEX: FEMALE DATE OF DEATH: 01/17/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 01/17/00 15:17 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 638 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: 260.4 GRAMS PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY LUNG (LU) : -LIGHT FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE TAN AREAS, UP TO 10 MM IN DIAMETER PITUITARY (PI) : -MASS(ES); 10 X 8 X 5 MM, DARK RED AND SOFT SKIN (SK) : -ABRASION(S)/ULCERATION(S); RIGHT SHOULDER REGION: MULTIPLE DRY BROWN ABRADED AREAS, UP TO 2 MM IN DIAMETER (CAP 22) -CRUSTED AREA(S); MID DORSAL CERVICAL REGION: MULTIPLE DRY BROWN CRUSTED AREAS, UP TO 2 MM IN DIAMETER (CAP 21) VAGINA (VA) : -SEMIFLUID MATERIAL; WITHIN LUMEN: GREEN MUCOID MATERIAL MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,-MODERATE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),SLIGHT LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL -INFILTRATE, MACROPHAGE, ALVEOLAR,MODERATE PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL SKIN (SK) : -INFLAMMATION, CHRONIC,-MODERATE MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT VAGINA (VA) : -LUMEN, EXUDATE,-PRESENT -HYPERPLASIA, MUCINOUS,-MODERATE 3670 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1909 ANIMAL NUMBER: C93119 SEX: FEMALE DATE OF DEATH: 01/17/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 01/17/00 15:17 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 638 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: 260.4 GRAMS PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL -LARGE; BILATERAL: EACH IS 10 X 5 X 3 MM; COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL, UPPER INCISORS -DISCHARGE-OCULAR; BILATERAL: DRY RED MATERIAL -ANIMAL THIN CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT LN, MANDIBULAR (MN) : -HYPERPLASIA, LYMPHOPLASMACYTIC,MODERATELY SEVERE -HEMORRHAGE,-PRESENT 3671 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1910 ANIMAL NUMBER: C93119 SEX: FEMALE DATE OF DEATH: 01/17/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 01/17/00 15:17 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 638 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: 260.4 GRAMS PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MEs /pANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: KIDNEY (KD), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), HEMATO NEOPLASIA (HN) 3672 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1911 ANIMAL NUMBER: C93120 SEX: FEMALE DATE OF DEATH: 04/20/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/20/99 9:15 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 285.3 GRAMS PROSECTOR: GREGORY CODDINGTON RECORDER: TONY HANFELD PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) LF ADRENAL (AD0) RT ADRENAL (AD1) BRAIN (BR) LF KIDNEY (KD0) RT KIDNEY (KD1) LIVER (LI) LUNG (LU) LF OVARY (OV0) RT OVARY (OV1) SPLEEN (SP) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) UTERUS (UT) .0343 .0334 2.0929 1.2810 1.2307 12.5676 1.6572 .0448 .0377 .4264 .0083 .0103 1.3539 .0120 % .0117 % .7336 % .4490 % .4314 % 4.4050 % .5809 % .0157 % .0132 % .1495 % .0029 % .0036 % .4746 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0164 .0160 1.0000 .6121 .5880 6.0049 .7918 .0214 .0180 .2037 .0040 .0049 .6469 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-MODERATE -PIGMENT, HEPATOCELLULAR, CENTRILOBULAR,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE 3673 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1912 ANIMAL NUMBER: C93120 SEX: FEMALE DATE OF DEATH: 04/20/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/20/99 9:15 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 285.3 GRAMS PROSECTOR: GREGORY CODDINGTON RECORDER: TONY HANFELD PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY LUNG (LU) : -LIGHT FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE TAN AREAS, UP TO 3 X 2 MM PITUITARY (PI) : -RED FOCUS(I)/AREA(S); RIGHT ASPECT: SINGLE DARK RED FOCUS, 1 MM IN DIAMETER GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MINIMAL ADRENAL, CORTEX (AC) : -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,SLIGHT PITUITARY (PI) : -HYPERPLASIA,-MODERATE -ANGIECTASIS,-PRESENT MAMMARY, FEMALE (MF) : -SECRETORY MATERIAL,-PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 3674 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1913 ANIMAL NUMBER: C93120 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 285.3 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 9:15 PROSECTOR: GREGORY CODDINGTON RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 3675 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1914 ANIMAL NUMBER: C93121 SEX: FEMALE DATE OF DEATH: 10/20/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 10/20/99 7:40 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 549 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: 158.0 GRAMS PROSECTOR: JARED FISHEL RECORDER: JARED FISHEL PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; ROUGH HAIR COAT; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; HEAD TILT; LEFT; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; TREMORS; ENTIRE BODY; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; COLD TO TOUCH; ENTIRE BODY; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) MARROW, STERNUM (SE) : >NOTE:>TRIMMING COMMENT: MISSING FROM WET TISSUE HISTOPATHOLOGY BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, PELVIC,-MINIMAL -PIGMENT, TUBULAR,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-SLIGHT ADRENAL, CORTEX (AC) : -PIGMENT,-MINIMAL MARROW, STERNUM (SE) : >TISSUE MISSING 3676 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1915 ANIMAL NUMBER: C93121 SEX: FEMALE DATE OF DEATH: 10/20/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 10/20/99 7:40 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 549 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: 158.0 GRAMS PROSECTOR: JARED FISHEL RECORDER: JARED FISHEL PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY BONE, STERNUM (SB) : >NOTE:>TRIMMING COMMENT: MISSING FROM WET TISSUE MUSCLE, SKELETAL (SM) : -ATROPHY-DIFFUSE; COLLECTED ON ROUTINE SECTION PITUITARY (PI) : -MASS(ES); 12 X 9 X 8 MM, RED, DARK RED, GRAY, SOFT AND LOBULATED STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: MULTIPLE DARK BROWN AREAS, UP TO 8 X 2 MM BONE, STERNUM (SB) : >TISSUE MISSING THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),SLIGHT LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL MUSCLE, SKELETAL (SM) : -ATROPHY,-MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : -EROSION,-MINIMAL RECTUM (RE) : -PARASITISM,-PRESENT MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 3677 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1916 ANIMAL NUMBER: C93121 SEX: FEMALE DATE OF DEATH: 10/20/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 10/20/99 7:40 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 549 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: 158.0 GRAMS PROSECTOR: JARED FISHEL RECORDER: JARED FISHEL PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN -GI TRACT-DARK FLUID CONTENT; DARK GREEN SEMIFLUID JEJUNUM: THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), SPINAL CORD (SC), HEART (HT), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 3678 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1917 ANIMAL NUMBER: C93122 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/21/00 STUDY DAY OF DEATH: 642 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: 367.8 GRAMS DATE AND TIME OF NECROPSY: 01/21/00 14:33 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); VENTRAL-ABDOMINAL-RIGHT; WEEK 67; 60 X 40MM; VERIFIED AT NECROPSY: NOT OBSERVED >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK 71; 40 X 40MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); INGUINAL REGION-RIGHT; WEEK 72; 20 X 15MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); INGUINAL REGION-RIGHT; WEEK 78; 20 X 20MM ANTERIOR TO OTHER MASS; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >EXCRETION; DISCOLORED FECES; YELLOW IN COLOR; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) KIDNEY (KD) : >NOTE:>TRIMMING COMMENT: BILATERAL ADRENAL, CORTEX (AC) : -LARGE; RIGHT: 17 X 15 X 12 MM (LEFT IS 4 X 2 X 2 MM) HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-SLIGHT -GRANULAR CYTOPLASM, EOSINOPHILIC, CENTRILOBULAR,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MODERATELY SEVERE -MINERALIZATION, PELVIC,-MODERATE -HYPERPLASIA, TRANSITIONAL CELL,-SLIGHT PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-SEVERE ADRENAL, CORTEX (AC) : -HEMATOPOIESIS, EXTRAMEDULLARY,MODERATE -DEGENERATION, CYSTIC / HEMORRHAGIC,SEVERE -PIGMENT,-MODERATE -B-ADENOMA,-NEOPLASM PRESENT 3679 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1918 ANIMAL NUMBER: C93122 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/21/00 STUDY DAY OF DEATH: 642 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: 367.8 GRAMS DATE AND TIME OF NECROPSY: 01/21/00 14:33 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY OVARY (OV) : -CYST(S); LEFT: SINGLE CLEAR FLUID FILLED CYST, 4 MM IN DIAMETER PITUITARY (PI) : -MASS(ES); 9 X 7 X 6 MM, RED, TAN AND FIRM ADRENAL, CORTEX (AC) : -DEGENERATION / NECROSIS, UNILATERAL,SEVERE ADRENAL, MEDULLA (MA) : -B-PHEOCHROMOCYTOMA,- 2 NEOPLASMS PRESENT OVARY (OV) : -CYST,-PRESENT MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,-SLIGHT MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,MODERATELY SEVERE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATELY SEVERE LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL HEART (HT) : -CARDIOMYOPATHY,-SLIGHT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : -EROSION,-MINIMAL 3680 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1919 ANIMAL NUMBER: C93122 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/21/00 STUDY DAY OF DEATH: 642 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: 367.8 GRAMS DATE AND TIME OF NECROPSY: 01/21/00 14:33 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: MULTIPLE DARK BROWN LINEAR AREAS, UP TO 5 X 1 MM MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT INGUINAL: SINGLE MASS, 60 X 40 X 32 MM, RED, TAN, LOBULATED AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (INGUINAL) NOT IDENTIFIED; POSSIBLY CONSISTENT WITH ALL MASSES (RIGHT INGUINAL) NOTED IN CLINICAL OBSERVATION -MASS #2; LEFT INGUINAL: SINGLE MASS, 55 X 32 X 22 MM, RED, TAN, LOBULATED AND FIRM; SAME ON CUT SURFACE (CAP 21); REGIONAL LN (INGUINAL) NOT IDENTIFIED; POSSIBLY CONSISTENT WITH MASS (VENTRAL ABDOMINAL LEFT) NOTED IN CLINICAL OBSERVATION UTERUS (UT) : -CYST(S); LEFT, WITHIN WALL: SINGLE CLEAR FLUID FILLED CYST, 6 MM IN DIAMETER MAMMARY, FEMALE (MF) : -M-CARCINOMA,-NEOPLASM PRESENT -B-FIBROADENOMA,-NEOPLASM PRESENT >NOTE:>MASS #1 IS PRIMARILY A CARCINOMA, HOWEVER, THERE ARE ALSO AREAS OF FIBROADENOMA UTERUS (UT) : -DILATATION,-SEVERE VAGINA (VA) : -LUMEN, EXUDATE,-PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 3681 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1920 ANIMAL NUMBER: C93122 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/21/00 STUDY DAY OF DEATH: 642 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: 367.8 GRAMS DATE AND TIME OF NECROPSY: 01/21/00 14:33 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), LN, MESENTERIC (MS), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), HEMATO NEOPLASIA (HN) 3682 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1921 ANIMAL NUMBER: C93123 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 198.8 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 8:25 PROSECTOR: LYNN HORNER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN STATUS LF ADRENAL (AD0) RT ADRENAL (AD1) BRAIN (BR) LF KIDNEY (KD0) RT KIDNEY (KD1) LIVER (LI) LUNG (LU) LF OVARY (OV0) RT OVARY (OV1) SPLEEN (SP) LF THYROID/PARA RT THYROID/PARA UTERUS (UT) (TT0) (TT1) -------- 8.0297 -------- -------- -------- -------- 4.0391 % -------- -------- -------- -------- --------------- -------- -------- NOT TAKEN WEIGHT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS NOTTAK NOTTAK NOTTAK NOTTAK NOTTAK NOTTAK NOTTAK NOTTAK 3683 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1922 ANIMAL NUMBER: C93123 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 198.8 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 8:25 PROSECTOR: LYNN HORNER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) 3684 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1923 ANIMAL NUMBER: C93124 SEX: FEMALE DATE OF DEATH: 04/20/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/20/00 7:45 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 369.9 GRAMS PROSECTOR: JAMES T. CALLEN RECORDER: JERALD W. JOHNSON PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); VENTRAL-ABDOMINAL-RIGHT; WEEK 72; 70 X 60 MM; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; VENTRALABDOMINAL-LEFT; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); VENTRAL-ABDOMINAL-RIGHT; WEEK 94; 30 X 30 MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); SHOULDER-LEFT; WEEK 95; 10 X 10MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK 71; 30 X 40 MM; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); VENTRAL-THORACIC-RIGHT; WEEK 97; 20 X 15MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); AXILLARY REGION-LEFT; WEEK 102; 10 X 10MM; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-SLIGHT -GRANULAR CYTOPLASM, EOSINOPHILIC, CENTRILOBULAR,-SLIGHT -PIGMENT, HEPATOCELLULAR, CENTRILOBULAR,-MODERATE -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL -NECROSIS, COAGULATIVE,-MINIMAL LIVER-OIL RED O (LI0) : -VACUOLES, OIL RED O-POSITIVE, HEPATOCELLULAR,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, PELVIC,-MINIMAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATELY SEVERE PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-SEVERE 3685 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1924 ANIMAL NUMBER: C93124 SEX: FEMALE DATE OF DEATH: 04/20/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/20/00 7:45 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 369.9 GRAMS PROSECTOR: JAMES T. CALLEN RECORDER: JERALD W. JOHNSON PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY >MASS(ES); VENTRAL-CERVICAL-LEFT; WEEK 102; 10 X 10MM; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; BLUE SKIN; VENTRALABDOMINAL-LEFT; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; SORE/SCAB; VENTRALABDOMINAL-RIGHT; VERIFIED AT NECROPSY: OBSERVED OVARY (OV) : -CYST(S); BILATERAL: SINGLE CLEAR FLUID FILLED CYST ON EACH, LEFT IS 4 MM IN DIAMETER, RIGHT IS 3 MM IN DIAMETER LUNG (LU) : -LIGHT FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE TAN AREAS, UP TO 4 X 3 MM PITUITARY (PI) : -MASS(ES); 7 X 6 X 4 MM, RED, TAN AND SOFT OVARY (OV) : -CYST,-PRESENT MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,MODERATELY SEVERE MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,MODERATELY SEVERE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATELY SEVERE LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,MODERATE -INFILTRATE, LYMPHOHISTIOCYTIC,-SLIGHT HEART (HT) : -CARDIOMYOPATHY,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -M-CARCINOMA,- 6 NEOPLASMS PRESENT -B-FIBROADENOMA,- 2 NEOPLASMS PRESENT >NOTE:>MASSES 6 AND 7 ARE THE FIBROADENOMAS 3686 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1925 ANIMAL NUMBER: C93124 SEX: FEMALE DATE OF DEATH: 04/20/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/20/00 7:45 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 369.9 GRAMS PROSECTOR: JAMES T. CALLEN RECORDER: JERALD W. JOHNSON PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS(ES); LEFT AXILLARY REGION: SINGLE MASS, 23 X 16 X 11 MM, RED, TAN AND FIRM; SAME ON CUT SURFACE (CAP 22); REGIONAL LN (AXILLARY) NORMAL (CAP 24) -MASS #2; LEFT AXILLARY REGION, CAUDAL TO MASS #1: SINGLE MASS, 20 X 19 X 12 MM, TAN AND FIRM; SAME ON CUT SURFACE (CAP 23); REGIONAL LN (AXILLARY) NORMAL (CAP 24) -MASS #3; LEFT INGUINAL REGION: SINGLE MASS, 44 X 34 X 25 MM, TAN, RED, LOBULATED, VASCULAR AND FIRM; ON SECTION EXUDES A BROWN SEMIFLUID (CAP 28); REGIONAL LN (INGUINAL) NOT IDENTIFIED -MASS #4; RIGHT AXILLARY REGION: SINGLE MASS, 40 X 31 X 15 MM, RED, TAN, LOBULATED AND FIRM; SAME ON CUT SURFACE (CAP 27); REGIONAL LN (AXILLARY) NORMAL (CAP 24); OVERLYING SKIN HAS A MOIST RED ULCERATED AREA, 17 X 15 MM; COLLECTED INTACT WITH MASS -MASS #5; RIGHT AXILLARY REGION, CAUDAL TO MASS #4: SINGLE MASS, 34 X 25 X 15 MM, TAN, LOBULATED AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (AXILLARY) NORMAL (CAP 24) 3687 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1926 ANIMAL NUMBER: C93124 SEX: FEMALE DATE OF DEATH: 04/20/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/20/00 7:45 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 369.9 GRAMS PROSECTOR: JAMES T. CALLEN RECORDER: JERALD W. JOHNSON PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS #6; RIGHT INGUINAL REGION: SINGLE MASS, 75 X 65 X 34 MM, RED, DARK RED, TAN AND FIRM; SAME ON CUT SURFACE; PORTION COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED; OVERLYING SKIN HAS A SINGLE MOIST, RED ULCERATED AREA, 20 X 11 MM; COLLECTED INTACT WITH MASS -MASS #7; RIGHT INGUINAL REGION, CAUDAL TO MASS #6: SINGLE MASS, 21 X 15 X 12 MM; RED, TAN AND FIRM; SAME ON CUT SURFACE (CAP 25); REGIONAL LN (INGUINAL) NOT IDENTIFIED -MASS #8; RIGHT PERIANAL REGION: SINGLE MASS, 21 X 13 X 8 MM, TAN AND FIRM; SAME ON CUT SURFACE (CAP 26); REGIONAL LN (INGUINAL) NOT IDENTIFIED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN UTERUS (UT) : -HYPERPLASIA, CYSTIC, ENDOMETRIAL,MINIMAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, AXILLARY (AX) : -HYPERPLASIA, LYMPHOPLASMACYTIC,MODERATELY SEVERE 3688 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1927 ANIMAL NUMBER: C93124 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 369.9 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 7:45 PROSECTOR: JAMES T. CALLEN RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -ANIMAL THIN THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 3689 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1928 ANIMAL NUMBER: C93125 SEX: FEMALE DATE OF DEATH: 04/19/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/19/00 9:40 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 267.5 GRAMS PROSECTOR: DAVID SCHUETTE RECORDER: LAURIE J. SCHULLER PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -DIFFUSELY DARK; ALL LOBES: DARK BROWN -LIGHT FOCUS(I)/a REA(S); ALL LOBES: MULTIPLE TAN AREAS, UP TO 4 MM IN DIAMETER -MASS(ES); INVOLVING ENTIRE RIGHT LATERAL LOBE: SINGLE MASS, 35 X 25 X 15 MM, DARK BROWN AND SOFT; SAME ON CUT SURFACE (CAP 22); REGIONAL LN (HEPATIC) NORMAL (CAP 23) HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -GRANULAR CYTOPLASM, EOSINOPHILIC, CENTRILOBULAR,-MINIMAL -PIGMENT, HEPATOCELLULAR, CENTRILOBULAR,-SLIGHT -VACUOLATION, HEPATOCELLULAR, MIDZONAL / CENTRILOBULAR,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MODERATE -NECROSIS, COAGULATIVE,-MINIMAL -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL -HYPERPLASIA, BILE DUCT,-MODERATE -FIBROSIS, PERIBILIARY,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, PERIPORTAL,-MODERATELY SEVERE -INFILTRATE, MACROPHAGE, PIGMENTED,SLIGHT -VACUOLATION, HEPATOCELLULAR,-SLIGHT -B-ADENOMA, HEPATOCELLULAR,-NEOPLASM PRESENT 3690 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1929 ANIMAL NUMBER: C93125 SEX: FEMALE DATE OF DEATH: 04/19/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/19/00 9:40 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 267.5 GRAMS PROSECTOR: DAVID SCHUETTE RECORDER: LAURIE J. SCHULLER PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY ADRENAL, CORTEX (AC) : -SMALL; LEFT: 4 X 2 X 1 MM (RIGHT IS 5 X 5 X 3 MM) -DIFFUSELY DARK; BILATERAL: DARK BROWN PITUITARY (PI) : -MASS(ES); 7 X 7 X 4 MM, DARK RED AND SOFT MAMMARY, FEMALE (MF) : -THICKENED; ALL REGIONS DIFFUSE: TAN AND FIRM (CAP 24) LIVER-OIL RED O (LI0) : -VACUOLES, OIL RED O-POSITIVE, HEPATOCELLULAR,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, PELVIC,-MODERATE SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : -HYPERTROPHY, CORTICAL CELL,-MODERATE -DEGENERATION, CYSTIC / HEMORRHAGIC,MODERATE -PIGMENT,-MODERATE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MINIMAL HEART (HT) : -CARDIOMYOPATHY,-SLIGHT THYROID (TY) : -B-HIBERNOMA,-NEOPLASM PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT -B-FIBROADENOMA,-NEOPLASM PRESENT -HYPERPLASIA, GLANDULAR,-SLIGHT 3691 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1930 ANIMAL NUMBER: C93125 SEX: FEMALE DATE OF DEATH: 04/19/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/19/00 9:40 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 267.5 GRAMS PROSECTOR: DAVID SCHUETTE RECORDER: LAURIE J. SCHULLER PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY CERVIX (CV) : -MASS(ES); INVOLVING ENTIRE CERVIX: SINGLE MASS, 23 X 17 X 17 MM, RED, TAN, AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (SEE LUMBAR) LN, LUMBAR/ILIAC (LM) : -DIFFUSELY RED; RIGHT: DARK RED 25) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN (CAP CERVIX (CV) : -M-SARCOMA, STROMAL,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, OTHER (LN) : -HEMORRHAGE,-PRESENT -INFILTRATE, MACROPHAGE, PIGMENTED,MODERATELY SEVERE >NOTE:>HEPATIC LN, LUMBAR/ILIAC (LM) : -HEMORRHAGE,-PRESENT 3692 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1931 ANIMAL NUMBER: C93125 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 267.5 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 9:40 PROSECTOR: DAVID SCHUETTE RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) (SM), (SG), 3693 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1932 ANIMAL NUMBER: C93126 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/30/99 STUDY DAY OF DEATH: 406 STUDY WEEK OF DEATH: 58 TERMINAL BODY WEIGHT: 221.6 GRAMS DATE AND TIME OF NECROPSY: 05/30/99 10:56 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; ROUGH HAIR COAT; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; RECUMBENT; LATERAL; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; MODERATE; VERIFIED AT NECROPSY: NOT APPLICABLE >EYES; RED DISCHARGE; EYES; VERIFIED AT NECROPSY: OBSERVED >DISCHARGE; NASAL; RED IN COLOR; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) HISTOPATHOLOGY BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-MODERATE -GRANULAR CYTOPLASM, EOSINOPHILIC, CENTRILOBULAR,-SLIGHT -PIGMENT, HEPATOCELLULAR, CENTRILOBULAR,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, PELVIC,-SLIGHT -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL -INFLAMMATION, SUPPURATIVE, PELVIC,MODERATE SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE 3694 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1933 ANIMAL NUMBER: C93126 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/30/99 STUDY DAY OF DEATH: 406 STUDY WEEK OF DEATH: 58 TERMINAL BODY WEIGHT: 221.6 GRAMS DATE AND TIME OF NECROPSY: 05/30/99 10:56 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 12 X 11 X 9 MM, RED, GREY, TAN, LOBULATED AND SOFT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DRY, RED MATERIAL -ANIMAL THIN -DISCHARGE-NASAL; DRY, RED MATERIAL ADRENAL, CORTEX (AC) : -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT -B-ADENOMA,-NEOPLASM PRESENT VAGINA (VA) : -LUMEN, EXUDATE,-PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 3695 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1934 ANIMAL NUMBER: C93126 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/30/99 STUDY DAY OF DEATH: 406 STUDY WEEK OF DEATH: 58 TERMINAL BODY WEIGHT: 221.6 GRAMS DATE AND TIME OF NECROPSY: 05/30/99 10:56 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), HEMATO NEOPLASIA (HN) 3696 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1935 ANIMAL NUMBER: C93127 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 236.6 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 11:41 PROSECTOR: DONIA MEYER RECORDER: KIMBERLY DURLAND POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) LF ADRENAL (AD0) RT ADRENAL (AD1) BRAIN (BR) LF KIDNEY (KD0) RT KIDNEY (KD1) LIVER (LI) LUNG (LU) LF OVARY (OV0) RT OVARY (OV1) SPLEEN (SP) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) UTERUS (UT) .0366 .0326 2.0193 1.2174 1.3144 11.0447 1.3204 .0707 .0676 .4388 .0078 .0096 1.1344 .0155 % .0138 % .8535 % .5145 % .5555 % 4.6681 % .5581 % .0299 % .0286 % .1855 % .0033 % .0041 % .4795 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0181 .0161 1.0000 .6029 .6509 5.4696 .6539 .0350 .0335 .2173 .0039 .0048 .5618 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED UTERUS (UT) : -LUMEN FILLED WITH FLUID; BILATERAL: CLEAR FLUID LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-MINIMAL -VACUOLATION, HEPATOCELLULAR, MIDZONAL / CENTRILOBULAR,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT UTERUS (UT) : >NOT REQUIRED TO BE EXAMINED FOR ANIMAL 3697 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1936 ANIMAL NUMBER: C93127 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 236.6 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 11:41 PROSECTOR: DONIA MEYER RECORDER: KIMBERLY DURLAND POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV) 3698 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1937 ANIMAL NUMBER: C93128 SEX: FEMALE DATE OF DEATH: 03/13/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 03/13/00 7:53 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 694 STUDY WEEK OF DEATH: 100 TERMINAL BODY WEIGHT: 385.5 GRAMS PROSECTOR: TODD ACKER RECORDER: TODD ACKER PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >EYES; RED DISCHARGE; EYES; VERIFIED AT NECROPSY: OBSERVED >RESPIRATION; LABORED; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) HISTOPATHOLOGY BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -NECROSIS, INDIVIDUAL HEPATOCYTE,MINIMAL -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL -HYPERPLASIA, BILE DUCT,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SLIGHT -MINERALIZATION, TUBULAR,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SLIGHT PANCREAS (PA) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL ADRENAL, CORTEX (AC) : -HYPERPLASIA, CORTICAL,-SLIGHT ADRENAL, MEDULLA (MA) : -UNILATERALLY EXAMINED,-PRESENT OVARY (OV) : -CYST,-PRESENT 3699 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1938 ANIMAL NUMBER: C93128 SEX: FEMALE DATE OF DEATH: 03/13/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 03/13/00 7:53 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 694 STUDY WEEK OF DEATH: 100 TERMINAL BODY WEIGHT: 385.5 GRAMS PROSECTOR: TODD ACKER RECORDER: TODD ACKER PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY OVARY (OV) : -CYST(S); BILATERAL: SINGLE CLEAR FLUID FILLED CYST ON EACH, BOTH ARE 1 MM IN DIAMETER PITUITARY (PI) : -MASS(ES); 10 X 7 X 7 MM, DARK RED AND SOFT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DARK RED AND DRY PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT -HYPERPLASIA, GLANDULAR,-MODERATE CERVIX (CV) : -HYPERPLASIA, MUCINOUS,-SEVERE VAGINA (VA) : -HYPERPLASIA, MUCINOUS,-SEVERE CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 3700 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1939 ANIMAL NUMBER: C93128 SEX: FEMALE DATE OF DEATH: 03/13/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 03/13/00 7:53 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 694 STUDY WEEK OF DEATH: 100 TERMINAL BODY WEIGHT: 385.5 GRAMS PROSECTOR: TODD ACKER RECORDER: TODD ACKER PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), HEMATO NEOPLASIA (HN) 3701 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1940 ANIMAL NUMBER: C93129 SEX: FEMALE DATE OF DEATH: 03/13/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 03/13/00 8:18 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (D) DEATH: 694 STUDY WEEK OF DEATH: 100 TERMINAL BODY WEIGHT: 296.0 GRAMS PROSECTOR: TODD ACKER RECORDER: TODD ACKER PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >EYES; PROTRUDING; EYES; VERIFIED AT NECROPSY: NOT APPLICABLE >EYES; DILATED PUPIL; EYES; VERIFIED AT NECROPSY: NOT APPLICABLE >EYES; DISCOLORED/LIGHT RED; EYES; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) KIDNEY (KD) : -LARGE PELVIS(ES); BILATERAL HISTOPATHOLOGY BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-SLIGHT -GRANULAR CYTOPLASM, EOSINOPHILIC, CENTRILOBULAR,-SLIGHT -PIGMENT, HEPATOCELLULAR, CENTRILOBULAR,-SLIGHT -HYPERPLASIA, BILE DUCT,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -DILATATION, PELVIC,-SLIGHT -MINERALIZATION, PELVIC,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : -PIGMENT,-SLIGHT -FIBROSIS,-MINIMAL 3702 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1941 ANIMAL NUMBER: C93129 SEX: FEMALE DATE OF DEATH: 03/13/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 03/13/00 8:18 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (D) DEATH: 694 STUDY WEEK OF DEATH: 100 TERMINAL BODY WEIGHT: 296.0 GRAMS PROSECTOR: TODD ACKER RECORDER: TODD ACKER PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 11 X 10 X 7 MM, DARK RED, RED, TAN AND SOFT UTERUS (UT) : -THICKENED WALL; BILATERAL, DIFFUSE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY), MINIMAL LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL HEART (HT) : -CARDIOMYOPATHY,-MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT JEJUNUM (JE) : >AUTOLYTIC ILEUM (IL) : >AUTOLYTIC MAMMARY, FEMALE (MF) -CYST / GALACTOCELE,-PRESENT -HYPERPLASIA, GLANDULAR,-MINIMAL UTERUS (UT) : -HYPERPLASIA, CYSTIC, ENDOMETRIAL,MINIMAL CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 3703 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1942 ANIMAL NUMBER: C93129 SEX: FEMALE DATE OF DEATH: 03/13/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 03/13/00 8:18 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (D) DEATH: 694 STUDY WEEK OF DEATH: 100 TERMINAL BODY WEIGHT: 296.0 GRAMS PROSECTOR: TODD ACKER RECORDER: TODD ACKER PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUm /s KULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG), GENERAL COMMENT (GC) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) (SG), 3704 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1943 ANIMAL NUMBER: C93130 SEX: FEMALE DATE OF DEATH: 04/20/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/20/99 10:59 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 384.6 GRAMS PROSECTOR: NANCY DIEDRICH RECORDER: TONY HANFELD PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) LF ADRENAL (AD0) RT ADRENAL (AD1) BRAIN (BR) LF KIDNEY (KD0) RT KIDNEY (KD1) LIVER (LI) LUNG (LU) LF OVARY (OV0) RT OVARY (OV1) SPLEEN (SP) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) UTERUS (UT) .0281 .0275 2.2869 1.3284 1.2841 13.9723 1.5891 .0615 .0384 .7788 .0135 .0129 .5901 .0073 % .0072 % .5946 % .3454 % .3339 % 3.6329 % .4132 % .0160 % .0100 % .2025 % .0035 % .0034 % .1534 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0123 .0120 1.0000 .5809 .5615 6.1097 .6949 .0269 .0168 .3405 .0059 .0056 .2580 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; UNVERIFIED AT NECROPSY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -PIGMENT, HEPATOCELLULAR, CENTRILOBULAR,-MINIMAL -VACUOLATION, HEPATOCELLULAR,-MINIMAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL -MINERALIZATION, TUBULAR,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MINIMAL PANCREAS (PA) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL 3705 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1944 ANIMAL NUMBER: C93130 SEX: FEMALE DATE OF DEATH: 04/20/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/20/99 10:59 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 384.6 GRAMS PROSECTOR: NANCY DIEDRICH RECORDER: TONY HANFELD PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN ADRENAL, CORTEX (AC) : -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 3706 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1945 ANIMAL NUMBER: C93130 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 384.6 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 10:59 PROSECTOR: NANCY DIEDRICH RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 3707 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1946 ANIMAL NUMBER: C93131 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/14/00 STUDY DAY OF DEATH: 666 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: 344.2 GRAMS DATE AND TIME OF NECROPSY: 02/14/00 14:17 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); VENTRAL-ABDOMINAL-RIGHT; WEEK 58; 50 X 50MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); AXILLARY REGION-RIGHT; WEEK 68; 40 X 40MM; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; HUNCHED; VERIFIED AT NECROPSY: NOT APPLICABLE >EYES; RED DISCHARGE; EYES; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -RAISED FOCUS(I)/AREA(S); ALONG MARGIN OF LEFT LATERAL LOBE: SINGLE DARK BROWN, SLIGHTLY RAISED FOCUS, 4 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION HISTOPATHOLOGY BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-MODERATE -GRANULAR CYTOPLASM, EOSINOPHILIC, CENTRILOBULAR,-SLIGHT -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-MODERATE -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MODERATE -HYPERPLASIA, BILE DUCT,-MINIMAL -ANGIECTASIS,-SLIGHT -B-ADENOMA, HEPATOCELLULAR,-NEOPLASM PRESENT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, PELVIC,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE 3708 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1947 ANIMAL NUMBER: C93131 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/14/00 STUDY DAY OF DEATH: 666 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: 344.2 GRAMS DATE AND TIME OF NECROPSY: 02/14/00 14:17 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); SINGLE MASS, 10 X 6 X 6 MM, DARK RED, DARK BROWN, AND SOFT MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT AXILLARY REGION: SINGLE MASS, 50 X 45 X 30 MM, TAN, FIRM, AND VASCULAR; SOME AREAS EXUDE A WHITE CREAMY SEMIFLUID; COLLECTED; REGIONAL LN (AXILLARY) NORMAL (CAP 21) ADRENAL, CORTEX (AC) : -HYPERTROPHY, CORTICAL CELL,-MODERATELY SEVERE -DEGENERATION, CYSTIC / HEMORRHAGIC,MODERATELY SEVERE -PIGMENT,-SLIGHT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATE LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,SLIGHT HEART (HT) : -CARDIOMYOPATHY,-MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT -B-FIBROADENOMA,- 2 NEOPLASMS PRESENT 3709 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1948 ANIMAL NUMBER: C93131 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/14/00 STUDY DAY OF DEATH: 666 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: 344.2 GRAMS DATE AND TIME OF NECROPSY: 02/14/00 14:17 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS #2; RIGHT INGUINAL REGION: SINGLE MASS, 70 X 55 X 35 MM, RED, TAN, VASCULAR, AND FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DARK RED MATERIAL -ANIMAL THIN -DISCHARGE-NASAL; BILATERAL: DARK RED MATERIAL VAGINA (VA) : -LUMEN, EXUDATE,-PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT LN, AXILLARY (AX) : -HYPERPLASIA, LYMPHOPLASMACYTIC,MODERATE 3710 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1949 ANIMAL NUMBER: C93131 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/14/00 STUDY DAY OF DEATH: 666 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: 344.2 GRAMS DATE AND TIME OF NECROPSY: 02/14/00 14:17 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), HEMATO NEOPLASIA (HN) (ST), (SK), 3711 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1950 ANIMAL NUMBER: C93132 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/17/00 STUDY DAY OF DEATH: 729 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 264.9 GRAMS DATE AND TIME OF NECROPSY: 04/17/00 14:24 PROSECTOR: JODI THIEREN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; SORE/SCAB; PAW-HINDLEFT; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); VENTRAL-ABDOMINAL; WEEK 97; 30 X 40MM; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; HUNCHED; VERIFIED AT NECROPSY: NOT APPLICABLE >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) HISTOPATHOLOGY EYE (EY) : -DYSTROPHY, CORNEAL IN EARLY STAGES, CONSISTS PRIMARILY OF FOCAL MINERALIZATION,-SLIGHT -INFLAMMATION, ACUTE,-MINIMAL BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-SLIGHT -GRANULAR CYTOPLASM, EOSINOPHILIC, CENTRILOBULAR,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL -VACUOLATION, HEPATOCELLULAR,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MODERATE 3712 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1951 ANIMAL NUMBER: C93132 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/17/00 STUDY DAY OF DEATH: 729 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 264.9 GRAMS DATE AND TIME OF NECROPSY: 04/17/00 14:24 PROSECTOR: JODI THIEREN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY SPLEEN (SP) : -SMALL; 13 X 5 X 3 MM PITUITARY (PI) : -MASS(ES); 8 X 8 X 4 MM, RED, DARK RED, GREY, TAN, AND FIRM KIDNEY (KD) : -MINERALIZATION, TUBULAR,-MODERATE SPLEEN (SP) : >UNREMARKABLE PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-SEVERE -ATROPHY, ACINAR,-SLIGHT ADRENAL, CORTEX (AC) : -HYPERTROPHY, CORTICAL CELL,-SLIGHT -DEGENERATION, CYSTIC / HEMORRHAGIC,SLIGHT -PIGMENT,-SLIGHT OVARY (OV) : -HYPERPLASIA, GRANULOSA CELL,MODERATELY SEVERE THYMUS (TH) : >SECTION EXAMINED; TISSUE NOT PRESENT LUNG (LU) : -MINERALIZATION, SEPTAL,-SEVERE HEART (HT) : -CARDIOMYOPATHY,-SLIGHT -MINERALIZATION, VASCULAR,-SEVERE -MINERALIZATION,-MODERATE PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : -EROSION,-SLIGHT -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT 3713 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1952 ANIMAL NUMBER: C93132 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/17/00 STUDY DAY OF DEATH: 729 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 264.9 GRAMS DATE AND TIME OF NECROPSY: 04/17/00 14:24 PROSECTOR: JODI THIEREN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: MULTIPLE DARK BROWN AREAS, UP TO 3 X 2 MM MAMMARY, FEMALE (MF) : -MASS(ES); PERIANAL REGION: SINGLE MASS, 35 X 35 X 28 MM, RED, TAN AND FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (INGUINAL) NORMAL (CAP 21); POSSIBLY CONSISTENT WITH MASS (VENTRAL ABDOMINAL) AS NOTED IN CLINICAL OBSERVATIONS CERVIX (CV) : -LARGE; 10 MM IN DIAMETER VAGINA (VA) : -SEMIFLUID MATERIAL; WITHIN LUMEN: TAN MUCOID MATERIAL FOOT/FOOTPAD (FP) : -ABRASION(S)/ULCERATION(S); LEFT HINDFOOT: SINGLE DARK RED, MOIST ABRADED AREA, 3 MM IN DIAMETER; COLLECTED STOMACH, GL (ST) : -HYPERPLASIA, EPITHELIAL, GLANDULAR,MODERATE -MINERALIZATION, GLANDULAR MUCOSA,MODERATE MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT -HYPERPLASIA, GLANDULAR,-MODERATELY SEVERE -B-ADENOMA,-NEOPLASM PRESENT UTERUS (UT) : -HYPERPLASIA, CYSTIC, ENDOMETRIAL,SLIGHT CERVIX (CV) : -HYPERPLASIA, STROMAL,-MODERATE VAGINA (VA) : -LUMEN, EXUDATE,-PRESENT -HYPERPLASIA, MUCINOUS,-MODERATE CAUSE OF DEATH (CD) : -NEPHROPATHY,-PRESENT FOOT/FOOTPAD (FP) : -INFLAMMATION, CHRONIC-ACTIVE,MODERATELY SEVERE -ACANTHOSIS / HYPERKERATOSIS,-MODERATE 3714 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1953 ANIMAL NUMBER: C93132 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/17/00 STUDY DAY OF DEATH: 729 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 264.9 GRAMS DATE AND TIME OF NECROPSY: 04/17/00 14:24 PROSECTOR: JODI THIEREN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), HEMATO NEOPLASIA LN, INGUINAL (IN) (HN), 3715 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1954 ANIMAL NUMBER: C93133 SEX: FEMALE DATE OF DEATH: 04/21/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/21/00 8:08 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 399.6 GRAMS PROSECTOR: TROY ELLENBECKER RECORDER: TONE EVERTS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-MODERATE -GRANULAR CYTOPLASM, EOSINOPHILIC, CENTRILOBULAR,-SLIGHT -PIGMENT, HEPATOCELLULAR, CENTRILOBULAR,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : -HYPERTROPHY, CORTICAL CELL,-MINIMAL -PIGMENT,-SLIGHT ADRENAL, MEDULLA (MA) : -UNILATERALLY EXAMINED,-PRESENT MARROW, FEMUR (FM) : -HYPOCELLULAR, MARROW,-SLIGHT 3716 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1955 ANIMAL NUMBER: C93133 SEX: FEMALE DATE OF DEATH: 04/21/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/21/00 8:08 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 399.6 GRAMS PROSECTOR: TROY ELLENBECKER RECORDER: TONE EVERTS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -LARGE; 7 X 6 X 4 MM; APPEARS SWOLLEN GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),SLIGHT LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT ILEUM (IL) : -HYPERPLASIA, GALT (GUT ASSOCIATED LYMPHOID TISSUE),-MODERATE MAMMARY, FEMALE (MF) : -HYPERPLASIA, GLANDULAR,-SLIGHT UTERUS (UT) : -B-POLYP, ENDOMETRIAL, STROMAL,NEOPLASM PRESENT VAGINA (VA) : -LUMEN, EXUDATE,-PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 3717 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1956 ANIMAL NUMBER: C93133 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 399.6 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 8:08 PROSECTOR: TROY ELLENBECKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), HEMATO NEOPLASIA (HN) 3718 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1957 ANIMAL NUMBER: C93134 SEX: FEMALE DATE OF DEATH: 08/05/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 08/05/99 8:22 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (D) DEATH: 473 STUDY WEEK OF DEATH: 68 TERMINAL BODY WEIGHT: NOT ENTERED PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); SHOULDER-LEFT; WEEK 48; 100 X 100MM; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -GRANULAR CYTOPLASM, EOSINOPHILIC, CENTRILOBULAR,-SLIGHT -DEGENERATION / NECROSIS, CENTRILOBULAR,-SLIGHT KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL -INFLAMMATION, CHRONIC-ACTIVE, PELVIC,SLIGHT SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SLIGHT ADRENAL, CORTEX (AC) : -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL MARROW, FEMUR (FM) : -HYPERPLASIA, ERYTHROID, MARROW,MODERATE MARROW, STERNUM (SE) : -HYPERPLASIA, ERYTHROID, MARROW,MODERATE THYMUS (TH) : -HEMORRHAGE,-PRESENT 3719 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1958 ANIMAL NUMBER: C93134 SEX: FEMALE DATE OF DEATH: 08/05/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 08/05/99 8:22 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (D) DEATH: 473 STUDY WEEK OF DEATH: 68 TERMINAL BODY WEIGHT: NOT ENTERED PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: MULTIPLE BROWN AREAS, UP TO 3 X 2 MM MAMMARY, FEMALE (MF) : -MASS(ES); LEFT LATERAL THORACIC REGION: SINGLE MASS, 118 X 100 X 70 MM (379.6 GRAMS),RED, DARK RED, GRAY, TAN, LOBULATED, VASCULAR AND FIRM; SAME ON CUT SURFACE; PORTION COLLECTED; REGIONAL LN (AXILLARY) NORMAL (CAP 20) LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,SLIGHT -INFLAMMATION, VASCULAR,-SLIGHT PITUITARY (PI) : -HYPERPLASIA,-SLIGHT STOMACH, GL (ST) : >UNREMARKABLE MAMMARY, FEMALE (MF) : -B-FIBROADENOMA,-NEOPLASM PRESENT URINARY BLADDER (UB) : -HYPERPLASIA, TRANSITIONAL CELL,-SLIGHT -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE CAUSE OF DEATH (CD) : -NEOPLASM, MAMMARY GLAND,-PRESENT LN, AXILLARY (AX) : -HYPERPLASIA, LYMPHOPLASMACYTIC,-SLIGHT 3720 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1959 ANIMAL NUMBER: C93134 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 08/05/99 STUDY DAY OF DEATH: 473 STUDY WEEK OF DEATH: 68 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 08/05/99 8:22 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG), GENERAL COMMENT (GC) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 3721 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1960 ANIMAL NUMBER: C93135 SEX: FEMALE DATE OF DEATH: 04/19/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/19/00 10:43 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 438.1 GRAMS PROSECTOR: CHANESTA ACKER RECORDER: TERRI L. WILLIAMS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; MISSING; TEETH; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; PAWS-HIND; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY HISTOPATHOLOGY EYE (EY) : -DYSTROPHY, CORNEAL IN EARLY STAGES, CONSISTS PRIMARILY OF FOCAL MINERALIZATION,-MINIMAL LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-SLIGHT -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-MODERATE -GRANULAR CYTOPLASM, EOSINOPHILIC, CENTRILOBULAR,-SLIGHT -PIGMENT, HEPATOCELLULAR, CENTRILOBULAR,-SLIGHT -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MODERATE -HEMATOPOIESIS, EXTRAMEDULLARY,-SLIGHT -HYPERPLASIA, BILE DUCT,-SLIGHT -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL 3722 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1961 ANIMAL NUMBER: C93135 SEX: FEMALE DATE OF DEATH: 04/19/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/19/00 10:43 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 438.1 GRAMS PROSECTOR: CHANESTA ACKER RECORDER: TERRI L. WILLIAMS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY ADRENAL, CORTEX (AC) : -LARGE,: LEFT: 10 X 8 X 5 MM (RIGHT IS 5 X 4 X 2 MM) LIVER-OIL RED O (LI0) : -VACUOLES, OIL RED O-POSITIVE, HEPATOCELLULAR,-MODERATE KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, PELVIC,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SLIGHT PANCREAS (PA) : -INFILTRATION, FAT, INTERSTITIAL,SLIGHT ADRENAL, CORTEX (AC) -ANGIECTASIS,-SEVERE -PIGMENT,-SLIGHT MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,-MODERATE MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,-SLIGHT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),SLIGHT LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL 3723 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1962 ANIMAL NUMBER: C93135 SEX: FEMALE DATE OF DEATH: 04/19/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/19/00 10:43 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 438.1 GRAMS PROSECTOR: CHANESTA ACKER RECORDER: TERRI L. WILLIAMS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -DARK FOCUS(I)/AREA(S); LEFT ANTERIOR ASPECT: SINGLE DARK RED AREA, 2 MM IN DIAMETER MAMMARY, FEMALE (MF) : -CYST(S); ALL REGIONS, DIFFUSE: MULTIPLE TAN CREAMY FLUID FILLED CYSTS, UP TO 3 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION UTERUS (UT) : -CYST(S); BILATERAL, WITHIN WALLS: MULTIPLE CLEAR FLUID FILLED CYSTS, UP TO 3 MM IN DIAMETER LN, MEDIASTINAL (ML) : -LARGE; UP TO 10 X 5 X 2 MM (CAP 23) FOOT/FOOTPAD (FP) : -CRUSTED AREA(S); BILATERAL, HINDFEET: EACH HAS A SINGLE DARK RED, TAN, DRY CRUSTED AREA, LEFT IS 7 X 5 MM, RIGHT IS 4 X 2; BOTH COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN HEART (HT) : -CARDIOMYOPATHY,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT -HYPERPLASIA,-MINIMAL HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-SLIGHT MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT -HYPERPLASIA, GLANDULAR,-MINIMAL UTERUS (UT) : >UNREMARKABLE CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MEDIASTINAL (ML) : -HYPERPLASIA, LYMPHOPLASMACYTIC,MODERATE FOOT/FOOTPAD (FP) : -INFLAMMATION, CHRONIC-ACTIVE,MODERATELY SEVERE -ACANTHOSIS / HYPERKERATOSIS,-MODERATE -FIBROSIS,-MODERATELY SEVERE 3724 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1963 ANIMAL NUMBER: C93135 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 438.1 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 10:43 PROSECTOR: CHANESTA ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN -MALOCCLUSION; UPPER LEFT INCISOR -TEETH MISSING/BROKEN/WORN; UPPER RIGHT INCISOR, BROKEN AT BASE GENERAL INFORMATION (XX) : >NOTE:>ANIMAL IDENTIFICATION TRANSPONDER DAMAGED AT NECROPSY 3725 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1964 ANIMAL NUMBER: C93135 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 438.1 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 10:43 PROSECTOR: CHANESTA ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: BRAIN (BR), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 3726 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1965 ANIMAL NUMBER: C93136 SEX: FEMALE DATE OF DEATH: 05/09/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 05/09/99 9:50 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (D) DEATH: 385 STUDY WEEK OF DEATH: 55 TERMINAL BODY WEIGHT: NOT ENTERED PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -LARGE; ALL LOBES THICKENED, MARGINS ROUNDED -DIFFUSELY DARK; ALL LOBES: DARK BROWN -LIGHT FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE, TAN, FIRM, SLIGHTLY INDISTINCT AREAS, UP TO 3 MM IN DIAMETER; COLLECTED ON ROUTINE SECTIONS -MASS(ES); MARGIN OF LEFT LATERAL LOBE: SINGLE MASS, 5 MM IN DIAMETER, RED, SOFT, AND VASCULAR; SAME ON CUT SURFACE; COLLECTED ON ROUTINE SECTION; REGIONAL LN (SEE ANTERIOR MESENTERIC) -ROUGH SURFACE, DIFFUSE; ALL LOBES: ENTIRE LIVER HAS A LOBULATED APPEARANCE KIDNEY (KD) : -DIFFUSELY DARK; BILATERAL: DARK BROWN SPLEEN (SP) : -CYST(S); ALONG MARGIN: MULTIPLE RED FLUID FILLED CYSTS, UP TO 5 MM IN DIAMETER HISTOPATHOLOGY LIVER (LI) : -FIBROSIS, PERIBILIARY,-SEVERE -DEGENERATION / NECROSIS, CENTRILOBULAR,-SLIGHT -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE -ECTASIA, BILE DUCT,-SEVERE -STASIS, BILE,-PRESENT KIDNEY (KD) : -PIGMENT, TUBULAR,-SLIGHT -ECTASIA, TUBULAR,-MODERATE -CAST, PROTEINACEOUS,-MODERATE SPLEEN (SP) : -CYST, SEROSAL,-PRESENT 3727 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1966 ANIMAL NUMBER: C93136 SEX: FEMALE DATE OF DEATH: 05/09/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 05/09/99 9:50 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (D) DEATH: 385 STUDY WEEK OF DEATH: 55 TERMINAL BODY WEIGHT: NOT ENTERED PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY OVARY (OV) : -MASS(ES); LEFT, INVOLVING ENTIRE OVARY: SINGLE MASS, 20 MM IN DIAMETER, DARK RED AND SOFT; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (SEE RENAL) LUNG (LU) : -LIGHT FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE TAN FOCI, UP TO 1 MM IN DIAMETER STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA: SINGLE TAN, ERODED AREA, 2 MM IN DIAMETER ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-MINIMAL -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL OVARY (OV) : -HEMATOMA,-PRESENT THYMUS (TH) : -HEMORRHAGE,-PRESENT LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL -EDEMA, ALVEOLAR,-SLIGHT HEART (HT) : -CARDIOMYOPATHY,-MINIMAL STOMACH, NONGL (SU) : >UNREMARKABLE JEJUNUM (JE) : >AUTOLYTIC ILEUM (IL) : >AUTOLYTIC CECUM (CE) : >AUTOLYTIC COLON (CO) : >AUTOLYTIC 3728 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1967 ANIMAL NUMBER: C93136 SEX: FEMALE DATE OF DEATH: 05/09/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 05/09/99 9:50 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (D) DEATH: 385 STUDY WEEK OF DEATH: 55 TERMINAL BODY WEIGHT: NOT ENTERED PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY LN, MEDIASTINAL (ML) : -LARGE; UP TO 5 X 3 X 2 MM (CAP 20) -DIFFUSELY RED; DARK RED LN, RENAL (RL) : -LARGE; BILATERAL: EACH IS UP TO 8 X 5 X 3 MM (CAP 22) -DIFFUSELY RED; BILATERAL: DARK RED LN, ANT MES/PANC (AP) : -LARGE; ANTERIOR MESENTERIC: UP TO 10 X 7 X 5 MM (CAP 21) -DIFFUSELY RED; ANTERIOR MESENTERIC: DARK RED GENERAL COMMENT (GC) : -AUTOLYSIS-SEVERE CAUSE OF DEATH (CD) : -HEPATIC ANOMALY / OBSTRUCTIVE HEPATOPATHY,-PRESENT LN, MEDIASTINAL (ML) : -HEMORRHAGE,-PRESENT -HYPERPLASIA, LYMPHOPLASMACYTIC,-SLIGHT LN, RENAL (RL) : -HYPERPLASIA, LYMPHOPLASMACYTIC,MODERATELY SEVERE LN, ANT MES/PANC (AP) : -HEMORRHAGE,-PRESENT -HYPERPLASIA, LYMPHOPLASMACYTIC,MODERATELY SEVERE 3729 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1968 ANIMAL NUMBER: C93136 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 05/09/99 STUDY DAY OF DEATH: 385 STUDY WEEK OF DEATH: 55 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 05/09/99 9:50 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), c a l v a r i u m /s k u l l (c a ), j o i n t , o t h e r (j t ), c l i t o r a l g l a n d (c l ), u r e t e r (u e ), a d i p o s e t i s s u e (a t ), d i a p h r a g m (d p ), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 3730 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1969 ANIMAL NUMBER: C93137 SEX: FEMALE DATE OF DEATH: 04/20/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/20/00 10:35 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 430.4 GRAMS PROSECTOR: JAMES T. CALLEN RECORDER: JERALD W. JOHNSON PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; PAWS-HIND; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; MISSING; TEETH; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE DARK RED AREAS, PINPOINT AND UP TO 3 X 2 MM; COLLECTED ON ROUTINE SECTIONS HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-SLIGHT -GRANULAR CYTOPLASM, EOSINOPHILIC, CENTRILOBULAR,-SLIGHT -PIGMENT, HEPATOCELLULAR, CENTRILOBULAR,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -HYPERPLASIA, BILE DUCT,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, TUBULAR,-MINIMAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : -PIGMENT,-MINIMAL OVARY (OV) : -CYST,-PRESENT 3731 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1970 ANIMAL NUMBER: C93137 SEX: FEMALE DATE OF DEATH: 04/20/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/20/00 10:35 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 430.4 GRAMS PROSECTOR: JAMES T. CALLEN RECORDER: JERALD W. JOHNSON PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: SINGLE DARK BROWN LINEAR AREA, 2 X 1 MM FOOT/FOOTPAD (FP) : -ABRASION(S)/ULCERATION(S); BILATERAL HINDFEET: SINGLE RED ABRADED AREA ON EACH, LEFT IS 4 MM IN DIAMETER, RIGHT IS 2 MM IN DIAMETER; COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -MALOCCLUSION; UPPER RIGHT INCISOR -TEETH MISSING/BROKEN/WORN; LEFT UPPER INCISOR: MISSING THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),SLIGHT THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PITUITARY (PI) : -HYPERPLASIA,-MINIMAL HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL STOMACH, GL (ST) : >UNREMARKABLE VAGINA (VA) : -HYPERPLASIA, MUCINOUS,-MODERATE CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT FOOT/FOOTPAD (FP) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE 3732 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1971 ANIMAL NUMBER: C93137 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 430.4 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 10:35 PROSECTOR: JAMES T. CALLEN RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB),UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN),TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB),UTERUS (UT), CERVIX (CV), HEMATO NEOPLASIA (HN) 3733 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1972 ANIMAL NUMBER: C93138 SEX: FEMALE DATE OF DEATH: 04/20/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/20/99 10:35 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 362.1 GRAMS PROSECTOR: KEVIN BILLINGS RECORDER: TONY HANFELD PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) LF ADRENAL (AD0) RT ADRENAL (AD1) BRAIN (BR) LF KIDNEY (KD0) RT KIDNEY (KD1) LIVER (LI) LUNG (LU) LF OVARY (OV0) RT OVARY (OV1) SPLEEN (SP) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) UTERUS (UT) .0282 .0301 2.1378 1.3720 1.3862 13.3847 1.6428 .0656 .0778 .5241 .0173 .0157 .6560 .0078 % .0083 % .5904 % .3789 % .3828 % 3.6964 % .4537 % .0181 % .0215 % .1447 % .0048 % .0043 % .1812 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0132 .0141 1.0000 .6418 .6484 6.2610 .7685 .0307 .0364 .2452 .0081 .0073 .3069 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-SLIGHT KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL -MINERALIZATION, TUBULAR,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE 3734 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1973 ANIMAL NUMBER: C93138 SEX: FEMALE DATE OF DEATH: 04/20/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/20/99 10:35 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 362.1 GRAMS PROSECTOR: KEVIN BILLINGS RECORDER: TONY HANFELD PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -RED FOCUS(I)/AREA(S); RIGHT ASPECT: SINGLE DARK RED SLIGHTLY RAISED AREA 2 MM IN DIAMETER GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN ADRENAL, CORTEX (AC) : -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL -HYPERTROPHY, CORTICAL CELL,-MINIMAL THYMUS (TH) : >SECTION EXAMINED; TISSUE NOT PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT -ANGIECTASIS,-PRESENT MAMMARY, FEMALE (MF) : -HYPERPLASIA, GLANDULAR,-SLIGHT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 3735 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1974 ANIMAL NUMBER: C93138 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 362.1 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 10:35 PROSECTOR: KEVIN BILLINGS RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 3736 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1975 ANIMAL NUMBER: C93139 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 300.0 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 8:08 PROSECTOR: DAVID SCHUETTE RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-SLIGHT -GRANULAR CYTOPLASM, EOSINOPHILIC, CENTRILOBULAR,-SLIGHT -PIGMENT, HEPATOCELLULAR, CENTRILOBULAR,-SLIGHT -NECROSIS, INDIVIDUAL HEPATOCYTE,MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SLIGHT -MINERALIZATION, PELVIC,-SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE 3737 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1976 ANIMAL NUMBER: C93139 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 300.0 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 8:08 PROSECTOR: DAVID SCHUETTE RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY ADRENAL, CORTEX (AC) : -LARGE; RIGHT: 8 X 6 X 5 MM (LEFT IS 5 X 5 X 4 MM) -RED FOCUS(I)/AREAS(I); BILATERAL: LEFT HAS A SINGLE DARK RED AREA, 3 MM IN DIAMETER, RIGHT HAS A MULTIPLE DARK RED AREAS, UP TO 7 X 4 MM PITUITARY (PI) : -DARK FOCUS(I)/AREA(S); LEFT MID ASPECT: SINGLE DARK BROWN FOCUS, 1 MM IN DIAMETER MAMMARY, FEMALE (MF) : -THICKENED; ALL REGIONS DIFFUSE: TAN; COLLECTED ON ROUTINE SECTION GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-SLIGHT ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC / HEMORRHAGIC,MODERATELY SEVERE -PIGMENT,-SLIGHT HEART (HT) : -M-ENDOCARDIAL SCHWANNOMA,-NEOPLASM PRESENT THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : >UNREMARKABLE RECTUM (RE) : -PARASITISM,-PRESENT MAMMARY, FEMALE (MF) : -HYPERPLASIA, GLANDULAR,-MODERATE CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 3738 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1977 ANIMAL NUMBER: C93139 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 300.0 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 8:08 PROSECTOR: DAVID SCHUETTE RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 3739 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1978 ANIMAL NUMBER: C93140 SEX: FEMALE DATE OF DEATH: 04/20/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/20/00 11:09 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 471.8 GRAMS PROSECTOR: GREGORY CODDINGTON RECORDER: TONE EVERTS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >EYES; RED DISCHARGE; EYE-RIGHT; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); VENTRAL-ABDOMINAL-RIGHT; WEEK 93; 15 X 20MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); AXILLARY REGION-RIGHT; WEEK 102; 10 X 10MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); INGUINAL REGION-RIGHT; WEEK 104; 10 X 10MM; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) HISTOPATHOLOGY EYE (EY) : -DYSTROPHY, CORNEAL IN EARLY STAGES, CONSISTS PRIMARILY OF FOCAL MINERALIZATION,-MINIMAL BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -NECROSIS, INDIVIDUAL HEPATOCYTE,SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MODERATE SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,MODERATELY SEVERE -ATROPHY, ACINAR,-MINIMAL -B-ADENOMA, ISLET CELL,-NEOPLASM PRESENT 3740 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1979 ANIMAL NUMBER: C93140 SEX: FEMALE DATE OF DEATH: 04/20/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/20/00 11:09 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 471.8 GRAMS PROSECTOR: GREGORY CODDINGTON RECORDER: TONE EVERTS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY OVARY (OV) : -CYST(S); RIGHT: SINGLE CLEAR FLUID FILLED CYST, 2 MM IN DIAMETER PITUITARY (PI) : -MASS(ES); 9 X 7 X 6 MM, RED, DARK RED, TAN, GREY, SOFT STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: SINGLE BROWN AREA, 3 X 2 MM ADRENAL, CORTEX (AC) : -HYPERTROPHY, CORTICAL CELL,-MINIMAL -DEGENERATION, CYSTIC / HEMORRHAGIC,MODERATE -PIGMENT,-SLIGHT ADRENAL, MEDULLA (MA) : -B-PHEOCHROMOCYTOMA,-NEOPLASM PRESENT OVARY (OV) : -CYST,-PRESENT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),SLIGHT HEART (HT) : -CARDIOMYOPATHY,-MODERATE THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : -INFLAMMATION, ACUTE,-MODERATE -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE -ULCERATION,-SLIGHT MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT -M-FIBROSARCOMA,- 4 NEOPLASMS PRESENT 3741 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1980 ANIMAL NUMBER: C93140 SEX: FEMALE DATE OF DEATH: 04/20/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/20/00 11:09 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 471.8 GRAMS PROSECTOR: GREGORY CODDINGTON RECORDER: TONE EVERTS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT AXILLARY REGION: SINGLE MASS, 15 X 15 X 10 MM, TAN, SOFT, AND LOBULATED; SAME ON CUT SURFACE (CAP 22); REGIONAL LN (AXILLARY) NORMAL (CAP 23) -MASS #2; RIGHT INGUINAL REGION: SINGLE MASS, 20 X 20 X 10 MM, TAN, SOFT, AND LOBULATED; SAME ON CUT SURFACE (CAP 24); REGIONAL LN (INGUINAL) NOT IDENTIFIED -MASS #3; RIGHT INGUINAL REGION, CAUDAL TO MASS #2: SINGLE MASS, 25 X 20 X 12 MM, TAN, GREY, FIRM; SAME ON CUT SURFACE (CAP 25); REGIONAL LN (INGUINAL) NOT IDENTIFIED -MASS #4; RIGHT VENTRAL ABDOMINAL REGION: SINGLE MASS, 35 X 22 X 15 MM, TAN, GREY, LOBULATED, AND FIRM; SAME ON CUT SURFACE (CAP 26); REGIONAL LN (INGUINAL) NOT IDENTIFIED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN UTERUS (UT) : -DILATATION,-MODERATE VAGINA (VA) : -LUMEN, EXUDATE,-PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 3742 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1981 ANIMAL NUMBER: C93140 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 471.8 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 11:09 PROSECTOR: GREGORY CODDINGTON RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN -DISCHARGE-OCULAR; RIGHT: DARK RED, AND DRY THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), HEMATO NEOPLASIA (HN), LN, AXILLARY (AX) 3743 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1982 ANIMAL NUMBER: C93141 SEX: FEMALE DATE OF DEATH: 03/17/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 03/17/00 9:43 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 698 STUDY WEEK OF DEATH: 100 TERMINAL BODY WEIGHT: 367.9 GRAMS PROSECTOR: TODD ACKER RECORDER: TODD ACKER PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; RED HAIR COAT; PERINEAL AREA; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; NO FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >EYES; RED DISCHARGE; EYE-RIGHT; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) HISTOPATHOLOGY BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-SLIGHT -GRANULAR CYTOPLASM, EOSINOPHILIC, CENTRILOBULAR,-SLIGHT -PIGMENT, HEPATOCELLULAR, CENTRILOBULAR,-SLIGHT -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-MODERATELY SEVERE -DEGENERATION, CYSTIC,-SLIGHT -HYPERPLASIA, BILE DUCT,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATE -HYPERTROPHY, CORTICAL CELL,-MINIMAL -PIGMENT,-MINIMAL 3744 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1983 ANIMAL NUMBER: C93141 SEX: FEMALE DATE OF DEATH: 03/17/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 03/17/00 9:43 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 698 STUDY WEEK OF DEATH: 100 TERMINAL BODY WEIGHT: 367.9 GRAMS PROSECTOR: TODD ACKER RECORDER: TODD ACKER PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 12 X 10 X 7 MM, DARK RED, RED AND SOFT MARROW, FEMUR (FM) : -HYPOCELLULAR, MARROW,-MODERATELY SEVERE MARROW, STERNUM (SE) : -HYPOCELLULAR, MARROW,-MODERATE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATE LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL -INFILTRATE, MACROPHAGE, ALVEOLAR,SLIGHT HEART (HT) : -CARDIOMYOPATHY,-MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -ATROPHY,-MINIMAL STOMACH, GL (ST) : -EDEMA,-MINIMAL -INFLAMMATION, ACUTE,-MINIMAL CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 3745 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1984 ANIMAL NUMBER: C93141 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/17/00 STUDY DAY OF DEATH: 698 STUDY WEEK OF DEATH: 100 TERMINAL BODY WEIGHT: 367.9 GRAMS DATE AND TIME OF NECROPSY: 03/17/00 9:43 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; RIGHT : DARK RED AND DRY -STAINS-PERINEUM/PERIANAL ; BROWN AND MOIST 3746 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1985 ANIMAL NUMBER: C93141 SEX: FEMALE DATE OF DEATH: 03/17/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 03/17/00 9:43 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 698 STUDY WEEK OF DEATH: 100 TERMINAL BODY WEIGHT: 367.9 GRAMS PROSECTOR: TODD ACKER RECORDER: TODD ACKER PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 3747 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1986 ANIMAL NUMBER: C93142 SEX: FEMALE DATE OF DEATH: 05/15/98 STUDY DAY OF DATE AND TIME OF NECROPSY: 05/15/98 9:31 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 210.2 GRAMS PROSECTOR: JODI THIEREN RECORDER: DEBBIE PIRKEL PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN STATUS LF ADRENAL (AD0) RT ADRENAL (AD1) BRAIN (BR) LF KIDNEY (KD0) RT KIDNEY (KD1) LIVER (LI) LUNG (LU) LF OVARY (OV0) RT OVARY (OV1) SPLEEN (SP) LF THYROID/PARA RT THYROID/PARA UTERUS (UT) (TT0) (TT1) -------- 8.2349 -------- -------- -------- -------- 3.9170 % -------- -------- -------- -------- --------------- -------- -------- NOT TAKEN WEIGHT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS NOTTAK NOTTAK NOTTAK NOTTAK NOTTAK NOTTAK NOTTAK NOTTAK 3748 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1987 ANIMAL NUMBER: C93142 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 210.2 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 9:31 PROSECTOR: JODI THIEREN RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) 3749 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1988 ANIMAL NUMBER: C93143 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 264.0 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 10:18 PROSECTOR: JODI THIEREN RECORDER: KIMBERLY DURLAND POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) LF ADRENAL (AD0) RT ADRENAL (AD1) BRAIN (BR) LF KIDNEY (KD0) RT KIDNEY (KD1) LIVER (LI) LUNG (LU) LF OVARY (OV0) RT OVARY (OV1) SPLEEN (SP) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) UTERUS (UT) .0368 .0317 2.1882 1.1879 1.2708 11.0191 1.2593 .1022 .0792 .3885 .0076 .0087 .4416 .0139 % .0120 % .8289 % .4500 % .4814 % 4.1739 % .4770 % .0387 % .0300 % .1472 % .0029 % .0033 % .1673 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0168 .0145 1.0000 .5429 .5808 5.0357 .5755 .0467 .0362 .1775 .0035 .0040 .2018 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-SLIGHT -NECROSIS, INDIVIDUAL HEPATOCYTE,MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT 3750 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1989 ANIMAL NUMBER: C93143 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 264.0 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 10:18 PROSECTOR: JODI THIEREN RECORDER: KIMBERLY DURLAND POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV) 3751 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1990 ANIMAL NUMBER: C93144 SEX: FEMALE DATE OF DEATH: 05/15/98 STUDY DAY OF DATE AND TIME OF NECROPSY: 05/15/98 11:10 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 175.5 GRAMS PROSECTOR: JODI THIEREN RECORDER: DEBBIE PIRKEL PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN STATUS LF ADRENAL (AD0) RT ADRENAL (AD1) BRAIN (BR) LF KIDNEY (KD0) RT KIDNEY (KD1) LIVER (LI) LUNG (LU) LF OVARY (OV0) RT OVARY (OV1) SPLEEN (SP) LF THYROID/PARA RT THYROID/PARA UTERUS (UT) (TT0) (TT1) -------- 7.9820 -------- -------- -------- -------- 4.5472 % -------- -------- -------- -------- --------------- -------- -------- NOT TAKEN WEIGHT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS NOTTAK NOTTAK NOTTAK NOTTAK NOTTAK NOTTAK NOTTAK NOTTAK 3752 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1991 ANIMAL NUMBER: C93144 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 175.5 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 11:10 PROSECTOR: JODI THIEREN RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) 3753 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1992 ANIMAL NUMBER: C93145 SEX: FEMALE DATE OF DEATH: 09/24/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 09/24/99 13:57 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (D) DEATH: 523 STUDY WEEK OF DEATH: 75 TERMINAL BODY WEIGHT: NOT ENTERED PROSECTOR: JARED FISHEL RECORDER: JARED FISHEL PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-MINIMAL -GRANULAR CYTOPLASM, EOSINOPHILIC, CENTRILOBULAR,-SLIGHT -PIGMENT, HEPATOCELLULAR, CENTRILOBULAR,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-SLIGHT -HYPERPLASIA, BILE DUCT,-MINIMAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL -HYPERPLASIA, TRANSITIONAL CELL,MINIMAL -INFLAMMATION, CHRONIC-ACTIVE, PELVIC,MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT 3754 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1993 ANIMAL NUMBER: C93145 SEX: FEMALE DATE OF DEATH: 09/24/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 09/24/99 13:57 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (D) DEATH: 523 STUDY WEEK OF DEATH: 75 TERMINAL BODY WEIGHT: NOT ENTERED PROSECTOR: JARED FISHEL RECORDER: JARED FISHEL PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY LN, MESENTERIC (MS) : -DIFFUSELY RED; BILATERAL: DARK RED PITUITARY (PI) : -MASS(ES); SINGLE MASS, 8 X 6 X 5 MM, DARK RED, TAN AND SOFT MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT PERIANAL REGION: SINGLE MASS, 30 X 20 X 15 MM, RED, DARK RED, GREEN, TAN, VASCULAR AND FIRM; SOME SECTIONS EXUDE A DARK RED FLUID; COLLECTED INTACT; REGIONAL LN (INGUINAL) NOT IDENTIFIED LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL: RED; COLLECTED ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT LN, MESENTERIC (MS) : >UNREMARKABLE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),SLIGHT LUNG (LU) : -CONGESTION,-PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT ILEUM (IL) : >AUTOLYTIC CECUM (CE) : >AUTOLYTIC MAMMARY, FEMALE (MF) : -M-CARCINOMA,-NEOPLASM PRESENT -CYST / GALACTOCELE,-PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT LN, MANDIBULAR (MN) : -HYPERPLASIA, LYMPHOPLASMACYTIC,MODERATE 3755 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1994 ANIMAL NUMBER: C93145 SEX: FEMALE DATE OF DEATH: 09/24/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 09/24/99 13:57 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (D) DEATH: 523 STUDY WEEK OF DEATH: 75 TERMINAL BODY WEIGHT: NOT ENTERED PROSECTOR: JARED FISHEL RECORDER: JARED FISHEL PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), TAIL (TI), LN, LUMBAr /i LIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG), GENERAL COMMENT (GC) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) (SN), 3756 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1995 ANIMAL NUMBER: C93146 SEX: FEMALE DATE OF DEATH: 04/20/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/20/00 8:16 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 326.2 GRAMS PROSECTOR: TROY ELLENBECKER RECORDER: JERALD W. JOHNSON PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK 100; 20 X 20 MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); AXILLARY REGION-RIGHT; WEEK 102; 10 X 10MM; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) KIDNEY (KD) : -LARGE PELVIS(ES); RIGHT HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -GRANULAR CYTOPLASM, EOSINOPHILIC, CENTRILOBULAR,-MINIMAL -PIGMENT, HEPATOCELLULAR, CENTRILOBULAR,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-SLIGHT -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SLIGHT -DILATATION, PELVIC,-MODERATE -MINERALIZATION, PELVIC,-SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATELY SEVERE PANCREAS (PA) : -ATROPHY, ACINAR,-MINIMAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT -HYPERTROPHY, CORTICAL CELL,-MODERATE 3757 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1996 ANIMAL NUMBER: C93146 SEX: FEMALE DATE OF DEATH: 04/20/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/20/00 8:16 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 326.2 GRAMS PROSECTOR: TROY ELLENBECKER RECORDER: JERALD W. JOHNSON PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY ADRENAL, CORTEX (AC) : -RED FOCUS(I)/AREAS(I); RIGHT CORTICAL SURFACE: SINGLE RED AREA, 4 MM IN DIAMETER OVARY (OV) : -CYST(S); RIGHT: SINGLE CLEAR FLUID CYST, 8 MM IN DIAMETER PITUITARY (PI) : -MASS(ES); 10 X 8 X 6 MM, DARK RED AND SOFT MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT AXILLARY REGION: SINGLE MASS, 20 X 15 X 7 MM, TAN AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (AXILLARY) NORMAL (CAP 22) -MASS #2; LEFT INGUINAL REGION: SINGLE MASS, 30 X 12 X 8 MM, TAN AND FIRM; SAME ON CUT SURFACE; SOME SECTIONS EXUDE A TAN, CREAMY FLUID; COLLECTED INTACT; REGIONAL LN (INGUINAL) NOT IDENTIFIED ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC / HEMORRHAGIC,MODERATE -PIGMENT,-MINIMAL LN, MESENTERIC (MS) : -HEMORRHAGE,-PRESENT OVARY (OV) : -CYST,-PRESENT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),SLIGHT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT -B-FIBROADENOMA,- 2 NEOPLASMS PRESENT -HYPERPLASIA, GLANDULAR,-MODERATE 3758 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1997 ANIMAL NUMBER: C93146 SEX: FEMALE DATE OF DEATH: 04/20/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/20/00 8:16 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 326.2 GRAMS PROSECTOR: TROY ELLENBECKER RECORDER: JERALD W. JOHNSON PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, AXILLARY (AX) : >TISSUE MISSING 3759 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1998 ANIMAL NUMBER: C93146 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 326.2 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 8:16 PROSECTOR: TROY ELLENBECKER RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 3760 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 1999 ANIMAL NUMBER: C93147 SEX: FEMALE DATE OF DEATH: 04/20/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/20/99 10:16 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 289.8 GRAMS PROSECTOR: NANCY DIEDRICH RECORDER: TONY HANFELD PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) LF ADRENAL (AD0) RT ADRENAL (AD1) BRAIN (BR) LF KIDNEY (KD0) RT KIDNEY (KD1) LIVER (LI) LUNG (LU) LF OVARY (OV0) RT OVARY (OV1) SPLEEN (SP) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) UTERUS (UT) .0215 .0259 2.3568 1.2275 1.2747 10.1724 1.6689 .0595 .0654 .6045 .0150 .0175 .6163 .0074 % .0089 % .8133 % .4236 % .4399 % 3.5101 % .5759 % .0205 % .0226 % .2086 % .0052 % .0060 % .2127 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0091 .0110 1.0000 .5208 .5409 4.3162 .7081 .0252 .0277 .2565 .0064 .0074 .2615 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-SLIGHT -PIGMENT, HEPATOCELLULAR, CENTRILOBULAR,-MINIMAL -NECROSIS, INDIVIDUAL HEPATOCYTE,MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL KIDNEY (KD) : -MINERALIZATION, TUBULAR,-MINIMAL 3761 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2000 ANIMAL NUMBER: C93147 SEX: FEMALE DATE OF DEATH: 04/20/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/20/99 10:16 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 289.8 GRAMS PROSECTOR: NANCY DIEDRICH RECORDER: TONY HANFELD PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL: (CAP 20) GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN DARK RED SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MINIMAL ADRENAL, CORTEX (AC) : -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,SLIGHT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MANDIBULAR (MN) : -HEMORRHAGE,-PRESENT 3762 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2001 ANIMAL NUMBER: C93147 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 289.8 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 10:16 PROSECTOR: NANCY DIEDRICH RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), TAIL (TI), LN, LUMBAr /i LIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 3763 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2002 ANIMAL NUMBER: C93148 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 01/15/00 STUDY DAY OF DEATH: 636 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 01/15/00 8:18 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -NECROSIS, INDIVIDUAL HEPATOCYTE,MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, PERIPORTAL,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, PELVIC,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT PANCREAS (PA) : -M-CARCINOMA, ISLET CELL,-NEOPLASM PRESENT >NOTE:>SEE SLIDE #21 ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATELY SEVERE -PIGMENT,-SLIGHT 3764 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2003 ANIMAL NUMBER: C93148 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 01/15/00 STUDY DAY OF DEATH: 636 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 01/15/00 8:18 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -RED FOCUS(I)/AREA(S); LEFT POSTERIOR ASPECT: SINGLE DARK RED FOCUS, 1 MM IN DIAMETER JEJUNUM (JE) : -MASS(ES); ATTACHED TO JEJUNUM, PROXIMAL TO DUODENUM: SINGLE MASS, 20 X 15 X 10 MM, DARK RED, TAN, AND SOFT; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (MESENTERIC) NORMAL MARROW, FEMUR (FM) : -HYPERPLASIA, ERYTHROID, MARROW,-SLIGHT MARROW, STERNUM (SE) : -HYPERPLASIA, ERYTHROID, MARROW,-SLIGHT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MINIMAL PITUITARY (PI) : -HYPERPLASIA,-SLIGHT HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-SLIGHT DUODENUM (DU) : >AUTOLYTIC JEJUNUM (JE) : >AUTOLYTIC ILEUM (IL) : >AUTOLYTIC CECUM (CE) : >AUTOLYTIC COLON (CO) : >AUTOLYTIC 3765 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2004 ANIMAL NUMBER: C93148 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 01/15/00 STUDY DAY OF DEATH: 636 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 01/15/00 8:18 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY CAUSE OF DEATH (CD) : -NEOPLASM, PANCREAS,-PRESENT THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG), GENERAL COMMENT (GC) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 3766 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2005 ANIMAL NUMBER: C93149 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 309.8 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 8:12 PROSECTOR: GREGORY CODDINGTON RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); AXILLARY REGION-LEFT; WEEK 91; 20 X 20MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); VENTRAL-CERVICAL-LEFT; WEEK 100; 15 X 15MM; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-SLIGHT -GRANULAR CYTOPLASM, EOSINOPHILIC, CENTRILOBULAR,-MINIMAL -NECROSIS, INDIVIDUAL HEPATOCYTE,MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-SLIGHT -HYPERPLASIA, BILE DUCT,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, PERIPORTAL,-SLIGHT -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL LIVER-OIL RED O (LI0) : -VACUOLES, OIL RED O-POSITIVE, HEPATOCELLULAR,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, PELVIC,-SLIGHT 3767 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2006 ANIMAL NUMBER: C93149 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 309.8 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 8:12 PROSECTOR: GREGORY CODDINGTON RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY OVARY (OV) : -CYST(S); RIGHT: SINGLE CLEAR FLUID FILLED CYST, 3 MM IN DIAMETER PITUITARY (PI) : -MASS(ES); 10 X 7 X 7 MM, RED, DARK RED, GREY AND SOFT SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : -HYPERTROPHY, CORTICAL CELL,-MODERATELY SEVERE -DEGENERATION, CYSTIC / HEMORRHAGIC,MODERATELY SEVERE -PIGMENT,-MINIMAL OVARY (OV) : -CYST,-PRESENT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATE LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, NONGL (SU) : -ACANTHOSIS / HYPERKERATOSIS,-MINIMAL -ULCERATION,-MINIMAL -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL MAMMARY, FEMALE (MF) : -M-CARCINOMA,- 2 NEOPLASMS PRESENT 3768 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2007 ANIMAL NUMBER: C93149 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 309.8 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 8:12 PROSECTOR: GREGORY CODDINGTON RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS(ES); LEFT AXILLARY REGION: SINGLE MASS, 28 X 20 X 15 MM, RED, DARK RED, TAN, BROWN AND FIRM; ON SECTION EXUDES A BROWN FLUID; (CAP 22); REGIONAL LN (AXILLARY) NORMAL (CAP 23) -MASS #2; LEFT VENTRAL CERVICAL REGION: SINGLE MASS, 25 X 15 X 12 MM, DARK RED, BROWN, TAN AND FIRM; SAME ON CUT SURFACE; (CAP 24); REGIONAL LN (MANDIBULAR) NORMAL (CAP 25) CAVITY, ABDOM (PC) : -MASS(ES); ATTACHED TO ADIPOSE TISSUE, NEAR RIGHT UTERINE HORN: SINGLE MASS, 20 X 10 X 8 MM, BROWN, TAN AND FIRM; SAME ON CUT SURFACE; (CAP 26); REGIONAL LN (LUMBAR) NORMAL (CAP 27) MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT -HYPERPLASIA, GLANDULAR,-SLIGHT UTERUS (UT) : -HYPERPLASIA, CYSTIC, ENDOMETRIAL,MINIMAL VAGINA (VA) : -LUMEN, EXUDATE,-PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, AXILLARY (AX) : -HYPERPLASIA, LYMPHOPLASMACYTIC,-SLIGHT LN, LUMBAR/ILIAC (LM) : -HYPERPLASIA, LYMPHOPLASMACYTIC,-SLIGHT CAVITY, ABDOM (PC) : -INFLAMMATION / NECROSIS, FAT,-MODERATE 3769 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2008 ANIMAL NUMBER: C93149 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 309.8 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 8:12 PROSECTOR: GREGORY CODDINGTON RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), HEMATO NEOPLASIA (HN), LN, MANDIBULAR (MN) 3770 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2009 ANIMAL NUMBER: C93150 SEX: FEMALE DATE OF DEATH: 01/19/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 01/19/00 14:21 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 640 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: 290.3 GRAMS PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; HEAD TILT; RIGHT; VERIFIED AT NECROPSY: NOT APPLICABLE >RESPIRATION; IRREGULAR; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; RECUMBENT; LATERAL; VERIFIED AT NECROPSY: NOT APPLICABLE >DISCHARGE; NASAL; RED IN COLOR; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) HISTOPATHOLOGY BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-MODERATE -GRANULAR CYTOPLASM, EOSINOPHILIC, CENTRILOBULAR,-SLIGHT -PIGMENT, HEPATOCELLULAR, CENTRILOBULAR,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, PELVIC,-MINIMAL -MINERALIZATION, TUBULAR,-SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : -PIGMENT,-SLIGHT 3771 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2010 ANIMAL NUMBER: C93150 SEX: FEMALE DATE OF DEATH: 01/19/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 01/19/00 14:21 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 640 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: 290.3 GRAMS PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 12 X 8 X 6 MM, DARK RED TAN AND FIRM GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-NASAL; DRY RED MATERIAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),SLIGHT -CYST,-PRESENT LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL HEART (HT) : -CARDIOMYOPATHY,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 3772 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2011 ANIMAL NUMBER: C93150 SEX: FEMALE DATE OF DEATH: 01/19/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 01/19/00 14:21 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 640 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: 290.3 GRAMS PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP),JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM(DP), CAVITY, THORACIC (TA),LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG),STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) (CE), 3773 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2012 ANIMAL NUMBER: C93151 SEX: FEMALE DATE OF DEATH: 04/21/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/21/00 10:31 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 492.0 GRAMS PROSECTOR: JOHN S. HALFORD RECORDER: LAURIE J. SCHULLER PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); INGUINAL REGION-LEFT; WEEK 79; 70 X 100MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); AXILLARY REGION-LEFT; WEEK 89; 30 X 20MM; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY ADRENAL, CORTEX (AC) : -LARGE; LEFT: 6 MM IN DIAMETER (RIGHT IS 4 MM IN DIAMETER) -DIFFUSELY DARK; BILATERAL: DARK BROWN HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-SLIGHT -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : -HYPERTROPHY, CORTICAL CELL,-SLIGHT -DEGENERATION, CYSTIC / HEMORRHAGIC,MODERATE -PIGMENT,-SLIGHT OVARY (OV) : -CYST,-PRESENT -HYPERPLASIA, GRANULOSA CELL,-MODERATE 3774 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2013 ANIMAL NUMBER: C93151 SEX: FEMALE DATE OF DEATH: 04/21/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/21/00 10:31 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 492.0 GRAMS PROSECTOR: JOHN S. HALFORD RECORDER: LAURIE J. SCHULLER PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY OVARY (OV) : -CYST(S); BILATERAL: SINGLE CLEAR FLUID FILLED CYST ON EACH, LEFT IS 6 MM IN DIAMETER, RIGHT IS 4 MM IN DIAMETER PITUITARY (PI) : -LARGE; 6 X 5 X 3 MM -DARK FOCUS(I)/AREA(S); ALONG ENTIRE LEFT ASPECT: MULTIPLE BLACK FOCI, UP TO 2 MM IN DIAMETER STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: TWO BLACK AREAS, ONE IS 3 X 1 MM, OTHER IS 2 X 1 MM MARROW, FEMUR (FM) : -HYPERPLASIA, ERYTHROID, MARROW,MODERATE MARROW, STERNUM (SE) : -HYPERPLASIA, ERYTHROID, MARROW,-SLIGHT LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT -HYPERPLASIA,-SLIGHT STOMACH, GL (ST) : -EROSION,-MINIMAL RECTUM (RE) : -PARASITISM,-PRESENT MAMMARY, FEMALE (MF) : -B-FIBROADENOMA,- 2 NEOPLASMS PRESENT 3775 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2014 ANIMAL NUMBER: C93151 SEX: FEMALE DATE OF DEATH: 04/21/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/21/00 10:31 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 492.0 GRAMS PROSECTOR: JOHN S. HALFORD RECORDER: LAURIE J. SCHULLER PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS(ES); INVOLVING ENTIRE INGUINAL REGION: SINGLE MASS, 110 X 83 X 50 MM (243.6 GRAMS), RED, DARK RED, GRAY, TAN, LOBULATED, VASCULAR, AND FIRM; SAME ON CUT SURFACE; PORTION COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED -MASS #2; LEFT AXILLARY REGION: SINGLE MASS, 21 X 20 X 13 MM, RED, GRAY, TAN, LOBULATED, AND FIRM; SAME ON CUT SURFACE (CAP 23); REGIONAL LN (SEE AXILLARY) UTERUS (UT) : -MASS(ES); LEFT WITHIN LUMEN, EXTENDING INTO BIFURCATION: SINGLE DARK RED AND GELATINOUS MASS, 11 X 10 X 9 MM; ON SECTION EXUDES A DARK RED GRANULAR MATERIAL; COLLECTED INTACT; REGIONAL LN (LUMBAR) NORMAL (CAP 25) LN, AXILLARY (AX) : -LARGE; LEFT: UP TO 6 X 4 X 3 MM (RIGHT IS UP TO 3 X 3 X 2 MM) (CAP 24) -DIFFUSELY RED; LEFT: DARK RED (CAP 24) UTERUS (UT) : -B-POLYP, ENDOMETRIAL, STROMAL,NEOPLASM PRESENT -HYPERPLASIA, CYSTIC, ENDOMETRIAL,SLIGHT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, AXILLARY (AX) : -HEMORRHAGE,-PRESENT -HYPERPLASIA, LYMPHOPLASMACYTIC,MODERATE LN, LUMBAR/ILIAC (LM) : -HYPERPLASIA, LYMPHOPLASMACYTIC,-SLIGHT 3776 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2015 ANIMAL NUMBER: C93151 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 492.0 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 10:31 PROSECTOR: JOHN S. HALFORD RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -ANIMAL THIN LN, LUMBAR/ILIAC (LM) : -HEMORRHAGE,-PRESENT 3777 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2016 ANIMAL NUMBER: C93151 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 492.0 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 10:31 PROSECTOR: JOHN S. HALFORD RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 3778 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2017 ANIMAL NUMBER: C93152 SEX: FEMALE DATE OF DEATH: 07/19/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 07/19/99 11:33 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 456 STUDY WEEK OF DEATH: 66 TERMINAL BODY WEIGHT: 253.7 GRAMS PROSECTOR: LYNN HORNER RECORDER: STEVE VAN ADESTINE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); AXILLARY REGION-RIGHT; WEEK 53; 25 X 25MM; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; HUNCHED; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; ATAXIC; VERIFIED AT NECROPSY: NOT APPLICABLE >DISCHARGE; NASAL; RED IN COLOR; VERIFIED AT NECROPSY: OBSERVED >EYES; RED DISCHARGE; EYES; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) HISTOPATHOLOGY BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-MODERATE -GRANULAR CYTOPLASM, EOSINOPHILIC, CENTRILOBULAR,-MINIMAL -PIGMENT, HEPATOCELLULAR, CENTRILOBULAR,-MINIMAL -NECROSIS, INDIVIDUAL HEPATOCYTE,MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-MINIMAL -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, TUBULAR,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE 3779 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2018 ANIMAL NUMBER: C93152 SEX: FEMALE DATE OF DEATH: 07/19/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 07/19/99 11:33 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 456 STUDY WEEK OF DEATH: 66 TERMINAL BODY WEIGHT: 253.7 GRAMS PROSECTOR: LYNN HORNER RECORDER: STEVE VAN ADESTINE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 15 X 8 X 5 MM, DARK RED, GRAY, AND SOFT MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT AXILLARY REGION: SINGLE MASS, 30 X 21 X 14 MM, DARK RED, TAN, LOBULATED, AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (AXILLARY) NORMAL (CAP 20) -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION -CYST(S); ALL REGIONS, DIFFUSE: MULTIPLE TAN, CREAMY SEMIFLUID FILLED CYSTS, UP TO 9 X 5 X 3 MM; COLLECTED ON ROUTINE SECTION GENERAL COMMENT (GC) : -BLOOD FILM TAKEN ADRENAL, CORTEX (AC) : -HYPERTROPHY, CORTICAL CELL,-MINIMAL -PIGMENT,-MINIMAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATE PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -M-CARCINOMA,-NEOPLASM PRESENT -CYST / GALACTOCELE,-PRESENT -INFLAMMATION, CHRONIC,-MODERATE CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT LN, AXILLARY (AX) : -INFILTRATE, MACROPHAGE, PIGMENTED,SLIGHT 3780 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2019 ANIMAL NUMBER: C93152 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/19/99 STUDY DAY OF DEATH: 456 STUDY WEEK OF DEATH: 66 TERMINAL BODY WEIGHT: 253.7 GRAMS DATE AND TIME OF NECROPSY: 07/19/99 11:33 PROSECTOR: LYNN HORNER RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -DISCHARGE-OCULAR; BILATERAL: DARK RED, DRY, CRUSTED MATERIAL -ANIMAL THIN -DISCHARGE-NASAL; DARK RED, DRY CRUSTED MATERIAL 3781 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2020 ANIMAL NUMBER: C93152 SEX: FEMALE DATE OF DEATH: 07/19/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 07/19/99 11:33 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 456 STUDY WEEK OF DEATH: 66 TERMINAL BODY WEIGHT: 253.7 GRAMS PROSECTOR: LYNN HORNER RECORDER: STEVE VAN ADESTINE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 3782 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2021 ANIMAL NUMBER: C93153 SEX: FEMALE DATE OF DEATH: 04/20/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/20/00 9:44 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 427.2 GRAMS PROSECTOR: JAMES T. CALLEN RECORDER: JERALD W. JOHNSON PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; SORE/SCAB; PAWS-HIND; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); DORSAL; WEEK 91; 40 X 40MM; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; SWOLLEN; PAW-HIND-LEFT; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-SLIGHT -GRANULAR CYTOPLASM, EOSINOPHILIC, CENTRILOBULAR,-MINIMAL -PIGMENT, HEPATOCELLULAR, CENTRILOBULAR,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-MODERATE KIDNEY (KD) : -MINERALIZATION, PELVIC,-SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SLIGHT PANCREAS (PA) : -INFILTRATION, FAT, INTERSTITIAL,SLIGHT ADRENAL, CORTEX (AC) : -HYPERTROPHY, CORTICAL CELL,-SLIGHT 3783 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2022 ANIMAL NUMBER: C93153 SEX: FEMALE DATE OF DEATH: 04/20/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/20/00 9:44 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 427.2 GRAMS PROSECTOR: JAMES T. CALLEN RECORDER: JERALD W. JOHNSON PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -LARGE; 7 X 6 X 4 MM -RED FOCUS(I)/AREA(S); DIFFUSE: MULTIPLE DARK RED AREAS, UP TO 2 X 1 MM MAMMARY, FEMALE (MF) : -MASS(ES); MID DORSAL THORACIC REGION: SINGLE MASS, 45 X 36 X 27 MM, TAN, LOBULATED AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (MANDIBULAR) NORMAL (CAP 22) ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC / HEMORRHAGIC,SLIGHT -PIGMENT,-SLIGHT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MINIMAL LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,MODERATE -INFILTRATE, LYMPHOHISTIOCYTIC,-SLIGHT HEART (HT) : -CARDIOMYOPATHY,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT -HYPERPLASIA,-MODERATE SKIN (SK) : -B-FIBROMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -HYPERPLASIA, GLANDULAR,-MODERATE >NOTE:>SEE SKIN FOR DIAGNOSIS OF MASS VAGINA (VA) : -HYPERPLASIA, MUCINOUS,-SLIGHT 3784 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2023 ANIMAL NUMBER: C93153 SEX: FEMALE DATE OF DEATH: 04/20/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/20/00 9:44 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 427.2 GRAMS PROSECTOR: JAMES T. CALLEN RECORDER: JERALD W. JOHNSON PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY FOOT/FOOTPAD (FP) : -ABRASION(S)/ULCERATION(S); BILATERAL, HINDFEET: SINGLE RED ABRADED AREA, LEFT IS 10 X 7 MM, RIGHT IS 3 MM IN DIAMETER; COLLECTED JOINT, OTHER (JT) : -LARGE; LEFT TARSAL JOINT: UP TO 10 MM IN DIAMETER GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MANDIBULAR (MN) : -HYPERPLASIA, LYMPHOPLASMACYTIC,MODERATELY SEVERE FOOT/FOOTPAD (FP) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE -ULCERATION,-MODERATE -ACANTHOSIS / HYPERKERATOSIS,-MODERATE -HYPERPLASIA, BONE,-MODERATE JOINT, OTHER (JT) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT 3785 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2024 ANIMAL NUMBER: C93153 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 427.2 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 9:44 PROSECTOR: JAMES T. CALLEN RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), HEMATO NEOPLASIA (HN) 3786 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2025 ANIMAL NUMBER: C93154 SEX: FEMALE DATE OF DEATH: 04/20/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/20/99 9:16 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 314.5 GRAMS PROSECTOR: NANCY DIEDRICH RECORDER: TONY HANFELD PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) LF ADRENAL (AD0) RT ADRENAL (AD1) BRAIN (BR) LF KIDNEY (KD0) RT KIDNEY (KD1) LIVER (LI) LUNG (LU) LF OVARY (OV0) RT OVARY (OV1) SPLEEN (SP) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) UTERUS (UT) .0398 .0328 2.3753 1.2854 1.4335 13.5315 1.5496 .0476 .0551 .5400 .0062 .0103 1.0091 .0127 % .0104 % .7553 % .4087 % .4558 % 4.3025 % .4927 % .0151 % .0175 % .1717 % .0020 % .0033 % .3209 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0168 .0138 1.0000 .5412 .6035 5.6968 .6524 .0200 .0232 .2273 .0026 .0043 .4248 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED LIVER (LI) : -DIFFUSELY DARK; ALL LOBES: DARK BROWN LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-MODERATE -PIGMENT, HEPATOCELLULAR, CENTRILOBULAR,-SLIGHT -NECROSIS, INDIVIDUAL HEPATOCYTE,MINIMAL KIDNEY (KD) : -MINERALIZATION, TUBULAR,-MINIMAL 3787 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2026 ANIMAL NUMBER: C93154 SEX: FEMALE DATE OF DEATH: 04/20/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/20/99 9:16 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 314.5 GRAMS PROSECTOR: NANCY DIEDRICH RECORDER: TONY HANFELD PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -LARGE; 5 X 5 X 3 MM; APPEARS SWOLLEN GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE PANCREAS (PA) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL ADRENAL, CORTEX (AC) : -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL -HYPERTROPHY, CORTICAL CELL,-MINIMAL ADRENAL, MEDULLA (MA) : -UNILATERALLY EXAMINED,-PRESENT THYMUS (TH) : -HEMORRHAGE,-PRESENT PITUITARY (PI) : >UNREMARKABLE MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT -HYPERPLASIA, GLANDULAR,-MINIMAL -SECRETORY MATERIAL,-PRESENT URINARY BLADDER (UB) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL UTERUS (UT) : -DILATATION,-SLIGHT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 3788 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2027 ANIMAL NUMBER: C93154 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 314.5 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 9:16 PROSECTOR: NANCY DIEDRICH RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) (SE), 3789 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2028 ANIMAL NUMBER: C93155 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 224.4 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 10:57 PROSECTOR: DONIA MEYER RECORDER: KIMBERLY DURLAND POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) LF ADRENAL (AD0) RT ADRENAL (AD1) BRAIN (BR) LF KIDNEY (KD0) RT KIDNEY (KD1) LIVER (LI) LUNG (LU) LF OVARY (OV0) RT OVARY (OV1) SPLEEN (SP) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) UTERUS (UT) .0387 .0397 1.7141 1.0546 1.0768 9.4384 1.3608 .0660 .0609 .4271 .0073 .0086 .5279 .0172 % .0177 % .7639 % .4700 % .4799 % 4.2061 % .6064 % .0294 % .0271 % .1903 % .0033 % .0038 % .2352 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0226 .0232 1.0000 .6152 .6282 5.5063 .7939 .0385 .0355 .2492 .0043 .0050 .3080 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 3790 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2029 ANIMAL NUMBER: C93155 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 224.4 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 10:57 PROSECTOR: DONIA MEYER RECORDER: KIMBERLY DURLAND POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV) 3791 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2030 ANIMAL NUMBER: C93156 SEX: FEMALE DATE OF DEATH: 12/06/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 12/06/99 15:21 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 596 STUDY WEEK OF DEATH: 86 TERMINAL BODY WEIGHT: 282.0 GRAMS PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; VENTRALCERVICAL; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); VENTRAL-CERVICAL; WEEK 53; 40 X 50MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); VENTRAL-ABDOMINAL; WEEK 84; 10 X 10MM; VERIFIED AT NECROPSY: OBSERVED >EYES; PROTRUDING; EYE-RIGHT; VERIFIED AT NECROPSY: OBSERVED >EYES; SCAB; EYE-RIGHT; VERIFIED AT NECROPSY: OBSERVED >EYES; PALE; EYE-RIGHT; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY EYE (EY) : -PROTRUDING; RIGHT: 4 MM FROM ORBIT; TISSUE SURROUNDING EYE IS DRY AND TAN; POSSIBLY CONSISTENT WITH SCAB (RIGHT EYE) AS NOTED IN CLINICAL OBSERVATIONS LIVER (LI) : -LIGHT FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE TAN AREAS, UP TO 8 X 3 MM; COLLECTED ON ROUTINE SECTIONS HISTOPATHOLOGY EYE (EY) : -INFLAMMATION, CHRONIC-ACTIVE,-SEVERE LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-SLIGHT -GRANULAR CYTOPLASM, EOSINOPHILIC, CENTRILOBULAR,-MINIMAL -PIGMENT, HEPATOCELLULAR, CENTRILOBULAR,-MINIMAL -NECROSIS, INDIVIDUAL HEPATOCYTE,MINIMAL -HYPERPLASIA, BILE DUCT,-SLIGHT -INFILTRATE, MACROPHAGE, PIGMENTED,SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, TUBULAR,-MINIMAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT 3792 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2031 ANIMAL NUMBER: C93156 SEX: FEMALE DATE OF DEATH: 12/06/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 12/06/99 15:21 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 596 STUDY WEEK OF DEATH: 86 TERMINAL BODY WEIGHT: 282.0 GRAMS PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY LN, MESENTERIC (MS) : -LARGE; UP TO 5 MM IN DIAMETER -DIFFUSELY RED; DARK RED LUNG (LU) : -LIGHT FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE TAN FOCI, UP TO 3 MM IN DIAMETER PITUITARY (PI) : -LARGE; 7 X 5 X 4 MM; APPEARS SWOLLEN -RED FOCUS(I)/AREA(S); RIGHT ANTERIOR ASPECT: SINGLE DARK RED FOCUS, 4 MM IN DIAMETER ADRENAL, CORTEX (AC) : -PIGMENT,-MINIMAL LN, MESENTERIC (MS) : -HEMORRHAGE,-PRESENT -HYPERPLASIA, LYMPHOPLASMACYTIC,-SEVERE BONE, FEMUR (FE) : -HYPERPLASIA, CARTILAGE,-SLIGHT MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,-SEVERE MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,-SEVERE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATELY SEVERE LUNG (LU) : -N-CARCINOMA,-FROM MAMMARY, FEMALE -INFILTRATE, MACROPHAGE, ALVEOLAR,SLIGHT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT 3793 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2032 ANIMAL NUMBER: C93156 SEX: FEMALE DATE OF DEATH: 12/06/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 12/06/99 15:21 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 596 STUDY WEEK OF DEATH: 86 TERMINAL BODY WEIGHT: 282.0 GRAMS PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY HARDERIAN GLAND (HG) : -MASS(ES); INVOLVING ENTIRE RIGHT: SINGLE MASS, 15 X 10 X 6 MM, RED, TAN, GREY, GELATINOUS AND SOFT; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (SEE MANDIBULAR) SALIV GL, MANDIB (SG) : -SMALL; LEFT: 11 X 8 X 3 MM (RIGHT IS 20 X 9 X 4 MM) MAMMARY, FEMALE (MF) : -MASS(ES); PERIANAL REGION: SINGLE TAN AND FIRM MASS, 22 X 16 X 11 MM; SAME ON CUT SURFACE (CAP 21); REGIONAL LN (INGUINAL) NOT IDENTIFIED; POSSIBLY CONSISTENT WITH MASS (VENTRAL ABDOMINAL REGION) AS NOTED IN CLINICAL OBSERVATIONS -MASS #2; VENTRAL CERVICAL REGION, EXTENDING TO LEFT PECTORAL REGION: SINGLE MASS, 77 X 38 X 30 MM, RED, DARK RED, GREY, TAN, LOBULATED, AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (SEE MANDIBULAR); OVERLYING SKIN HAS A SINGLE MOIST, RED AND TAN ERODED AREA, 21 X 20 MM; COLLECTED WITH MASS UTERUS (UT) : -THICKENED WALL; BILATERAL, DIFFUSE SALIV GL, MANDIB (SG) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -M-CARCINOMA,- 2 NEOPLASMS PRESENT UTERUS (UT) : >UNREMARKABLE 3794 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2033 ANIMAL NUMBER: C93156 SEX: FEMALE DATE OF DEATH: 12/06/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 12/06/99 15:21 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 596 STUDY WEEK OF DEATH: 86 TERMINAL BODY WEIGHT: 282.0 GRAMS PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY LN, MANDIBULAR (MN) : -LARGE; BILATERAL: EACH IS UP TO 11 X 7 X 6 MM (CAP 22) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN CERVIX (CV) : -HYPERPLASIA, MUCINOUS,-SEVERE VAGINA (VA) : -HYPERPLASIA, MUCINOUS,-SEVERE HEMATO NEOPLASIA (HN) : -M-SARCOMA, HISTIOCYTIC,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, MAMMARY GLAND,-PRESENT LN, MANDIBULAR (MN) : -HYPERPLASIA, LYMPHOPLASMACYTIC,-SEVERE 3795 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2034 ANIMAL NUMBER: C93156 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/06/99 STUDY DAY OF DEATH: 596 STUDY WEEK OF DEATH: 86 TERMINAL BODY WEIGHT: 282.0 GRAMS DATE AND TIME OF NECROPSY: 12/06/99 15:21 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), OVARY (OV), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB) 3796 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2035 ANIMAL NUMBER: C93157 SEX: FEMALE DATE OF DEATH: 04/21/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/21/00 8:12 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 537.9 GRAMS PROSECTOR: JAMES T. CALLEN RECORDER: LAURIE J. SCHULLER PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL -FIBROSIS, PERIBILIARY,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE PANCREAS (PA) : -B-FIBROMA,-NEOPLASM PRESENT ADRENAL, CORTEX (AC) : -PIGMENT,-MINIMAL ADRENAL, MEDULLA (MA) : -UNILATERALLY EXAMINED,-PRESENT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATELY SEVERE LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL 3797 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2036 ANIMAL NUMBER: C93157 SEX: FEMALE DATE OF DEATH: 04/21/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/21/00 8:12 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 537.9 GRAMS PROSECTOR: JAMES T. CALLEN RECORDER: LAURIE J. SCHULLER PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY UTERUS (UT) : -CYST(S); WITHIN WALL OF RIGHT HORN, 17 MM FROM BIFURCATION: SINGLE RED FLUID FILLED CYST, 2 MM IN DIAMETER CAVITY, ABDOM (PC) : -MASS(ES); WITHIN ADIPOSE TISSUE NEAR BIFURCATION OF UTERUS: SINGLE MASS, 25 X 20 X 14 MM, GREEN, LOBULATED, AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (LUMBAR) NORMAL (CAP 22) LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERPLASIA, TYPE II PNEUMOCYTE,MINIMAL HEART (HT) : -CARDIOMYOPATHY,-MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-SLIGHT MAMMARY, FEMALE (MF) : -HYPERPLASIA, GLANDULAR,-MINIMAL UTERUS (UT) : >UNREMARKABLE VAGINA (VA) : -HYPERPLASIA, MUCINOUS,-SLIGHT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, LUMBAR/ILIAC (LM) : -HYPERPLASIA, LYMPHOPLASMACYTIC,-SLIGHT CAVITY, ABDOM (PC) : -INFLAMMATION / NECROSIS, FAT,-SEVERE 3798 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2037 ANIMAL NUMBER: C93157 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 537.9 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 8:12 PROSECTOR: JAMES T. CALLEN RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), HEMATO NEOPLASIA (HN) 3799 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2038 ANIMAL NUMBER: C93158 SEX: FEMALE DATE OF DEATH: 04/19/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/19/00 10:13 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 448.8 GRAMS PROSECTOR: DAVID SCHUETTE RECORDER: LAURIE J. SCHULLER PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); VENTRAL-ABDOMINAL-RIGHT; WEEK 71; 70 X 60MM; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-SLIGHT -GRANULAR CYTOPLASM, EOSINOPHILIC, CENTRILOBULAR,-MODERATE -PIGMENT, HEPATOCELLULAR, CENTRILOBULAR,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MODERATE -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-SLIGHT -HYPERPLASIA, BILE DUCT,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, PELVIC,-SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE 3800 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2039 ANIMAL NUMBER: C93158 SEX: FEMALE DATE OF DEATH: 04/19/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/19/00 10:13 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 448.8 GRAMS PROSECTOR: DAVID SCHUETTE RECORDER: LAURIE J. SCHULLER PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY ADRENAL, CORTEX (AC) : -LARGE; LEFT: 7 X 5 X 3 MM (RIGHT IS 5 X 4 X 3 MM) -MOTTLED; BILATERAL: DARK BROWN AND TAN PITUITARY (PI) : -LARGE; 6 X 5 X 3 MM MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT INGUINAL REGION: SINGLE MASS, 90 X 60 X 25 MM (95.3 GRAMS), RED, TAN, SOFT, LOBULATED, AND VASCULAR; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (SEE INGUINAL) -MASS #2; RIGHT PERINEAL REGION: SINGLE MASS, 30 X 25 X 25 MM, TAN, DARK BROWN, AND FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (SEE INGUINAL) PANCREAS (PA) : -INFILTRATION, FAT, INTERSTITIAL,MINIMAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT -HYPERTROPHY, CORTICAL CELL,-SLIGHT -PIGMENT,-MINIMAL -B-ADENOMA,-NEOPLASM PRESENT BONE, STERNUM (SB) : -HYPERPLASIA, CARTILAGINOUS,-MODERATE HEART (HT) : -CARDIOMYOPATHY,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT -CYST,-PRESENT MAMMARY, FEMALE (MF) : -M-CARCINOMA,-NEOPLASM PRESENT -B-FIBROADENOMA,-NEOPLASM PRESENT -HYPERPLASIA, GLANDULAR,-SLIGHT >NOTE:>MASS #1 IS THE CARCINOMA CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 3801 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2040 ANIMAL NUMBER: C93158 SEX: FEMALE DATE OF DEATH: 04/19/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/19/00 10:13 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 448.8 GRAMS PROSECTOR: DAVID SCHUETTE RECORDER: LAURIE J. SCHULLER PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY LN, INGUINAL (IN) : -DIFFUSELY RED; RIGHT: DARK RED (CAP 22); LEFT (NOT IDENTIFIED) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN LN, INGUINAL (IN) : -INFILTRATE, MACROPHAGE, PIGMENTED,MODERATE -HEMORRHAGE,-PRESENT 3802 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2041 ANIMAL NUMBER: C93158 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 448.8 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 10:13 PROSECTOR: DAVID SCHUETTE RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 3803 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2042 ANIMAL NUMBER: C93159 SEX: FEMALE DATE OF DEATH: 05/15/98 STUDY DAY OF DATE AND TIME OF NECROPSY: 05/15/98 9:00 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 173.4 GRAMS PROSECTOR: LYNN HORNER RECORDER: DEBBIE PIRKEL PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN STATUS LF ADRENAL (AD0) RT ADRENAL (AD1) BRAIN (BR) LF KIDNEY (KD0) RT KIDNEY (KD1) LIVER (LI) LUNG (LU) LF OVARY (OV0) RT OVARY (OV1) SPLEEN (SP) LF THYROID/PARA RT THYROID/PARA UTERUS (UT) (TT0) (TT1) -------- 7.2825 -------- -------- -------- -------- 4.2009 % -------- -------- -------- -------- --------------- -------- -------- NOT TAKEN WEIGHT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS NOTTAK NOTTAK NOTTAK NOTTAK NOTTAK NOTTAK NOTTAK NOTTAK 3804 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2043 ANIMAL NUMBER: C93159 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 05/15/98 STUDY DAY OF DEATH: 26 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 173.4 GRAMS DATE AND TIME OF NECROPSY: 05/15/98 9:00 PROSECTOR: LYNN HORNER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: DEBBIE PIRKEL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) 3805 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2044 ANIMAL NUMBER: C93160 SEX: FEMALE DATE OF DEATH: 09/27/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 09/27/99 12:57 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 526 STUDY WEEK OF DEATH: 76 TERMINAL BODY WEIGHT: 311.5 GRAMS PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >WART LIKE LESION(S); NOSE; WEEK 72; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; YELLOW HAIR COAT; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; PAWS-HIND; VERIFIED AT NECROPSY: NOT OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; SWOLLEN; VENTRAL ABDOMINAL; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; COLD TO TOUCH; ENTIRE BODY; VERIFIED AT NECROPSY: NOT APPLICABLE >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY HISTOPATHOLOGY LIVER (LI) : -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-SLIGHT -HYPERPLASIA, BILE DUCT,-SLIGHT -FIBROSIS, PERIBILIARY,-SLIGHT -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL KIDNEY (KD) : -PIGMENT, TUBULAR,-MODERATE SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-MODERATE ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-MINIMAL -ANGIECTASIS,-MINIMAL -PIGMENT,-MINIMAL MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,-SLIGHT MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,-MODERATE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATELY SEVERE 3806 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2045 ANIMAL NUMBER: C93160 SEX: FEMALE DATE OF DEATH: 09/27/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 09/27/99 12:57 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 526 STUDY WEEK OF DEATH: 76 TERMINAL BODY WEIGHT: 311.5 GRAMS PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY SKIN (SK) : -CRUSTED AREA(S); ATTACHED TO RIGHT NOSTRIL: SINGLE BROWN, DRY, CRUSTED, WART-LIKE AREA, 5 X 1 X 1 MM (CAP 21) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN -STAINS-PERINEUM/PERIANAL; YELLOW MATERIAL MOIST THYMUS (TH) : -CYST,-PRESENT HEART (HT) : -CARDIOMYOPATHY,-SLIGHT -INFILTRATE, MACROPHAGE, PIGMENTED,SLIGHT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, NONGL (SU) : -ACANTHOSIS / HYPERKERATOSIS,-MINIMAL RECTUM (RE) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE -ULCERATION,-MODERATE SKIN (SK) : -ACANTHOSIS/HYPERKERATOSIS,-MODERATE -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE -SUPERFICIAL CRUSTING,-MINIMAL CAUSE OF DEATH (CD) : -INFLAMMATION / INFECTION,-PRESENT >NOTE:>PERITONITIS CAVITY, ABDOM (PC) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE 3807 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2046 ANIMAL NUMBER: C93160 SEX: FEMALE DATE OF DEATH: 09/27/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 09/27/99 12:57 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 526 STUDY WEEK OF DEATH: 76 TERMINAL BODY WEIGHT: 311.5 GRAMS PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -GI TRACT-LARGE LUMEN; INVOLVING CECUM, COLON, AND RECTUM: DISTENDED UP TO 27 MM IN DIAMETER AND FILLED WITH A HARD FECAL LIKE MATERIAL; POSSIBLY CONSISTENT WITH SWOLLEN ABDOMEN NOTED IN CLINICAL OBSERVATIONS 3808 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2047 ANIMAL NUMBER: C93160 SEX: FEMALE DATE OF DEATH: 09/27/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 09/27/99 12:57 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 526 STUDY WEEK OF DEATH: 76 TERMINAL BODY WEIGHT: 311.5 GRAMS PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), BONE, STERNUM SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) (SB), 3809 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2048 ANIMAL NUMBER: C93161 SEX: FEMALE DATE OF DEATH: 04/19/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/19/00 7:41 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 706.9 GRAMS PROSECTOR: CHANESTA ACKER RECORDER: TERRI L. WILLIAMS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; SORE/SCAB; PAW-HINDLEFT; VERIFIED AT NECROPSY: NOT OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -CYST(S); ALL LOBES: MULTIPLE CLEAR FLUID FILLED CYSTS, UP TO 1 MM IN DIAMETER; COLLECTED ON ROUTINE SECTIONS SPLEEN (SP) : -LARGE; 55 X 11 X 7 MM PANCREAS (PA) : -GELATINOUS; DIFFUSE: CLEAR AND TAN -LIGHT FOCUS(I)/AREA(S); DIFFUSE: MULTIPLE TAN AND FIRM AREAS, UP TO 4 MM IN DIAMETER HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-MODERATE -GRANULAR CYTOPLASM, EOSINOPHILIC, CENTRILOBULAR,-MINIMAL -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL -CYST, BILIARY,-PRESENT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATELY SEVERE PANCREAS (PA) : -FIBROSIS,-SEVERE -INFLAMMATION, CHRONIC-ACTIVE,-SEVERE ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-MINIMAL -HYPERTROPHY, CORTICAL CELL,-MINIMAL -PIGMENT,-MINIMAL 3810 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2049 ANIMAL NUMBER: C93161 SEX: FEMALE DATE OF DEATH: 04/19/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/19/00 7:41 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 706.9 GRAMS PROSECTOR: CHANESTA ACKER RECORDER: TERRI L. WILLIAMS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY LN, MESENTERIC (MS) : -LARGE; UP TO 20 X 13 X 11 MM; ALL APPEAR CYSTIC LUNG (LU) : -ADHESION(S); RIGHT MIDDLE LOBE ADHERED TO RIGHT CAUDAL LOBE: MULTIPLE GREY FIBROUS ADHESIONS CECUM (CE) : -LARGE; 60 X 30 X 7 MM; FILLED WITH A DARK GREEN, SOLID, FECAL-LIKE MATERIAL MAMMARY, FEMALE (MF) : -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION LN, MESENTERIC (MS) : -LYMPHANGIECTASIS,-MODERATE MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,MODERATELY SEVERE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATELY SEVERE LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,SLIGHT -FIBROSIS, PLEURAL/SUBPLEURAL,-SLIGHT PARATHYROID (PT) : >SECTION EXAMINED; TISSUE NOT PRESENT ILEUM (IL) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE CECUM (CE) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE COLON (CO) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE RECTUM (RE) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE MAMMARY, FEMALE (MF) : -HYPERPLASIA, GLANDULAR,-MODERATE 3811 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2050 ANIMAL NUMBER: C93161 SEX: FEMALE DATE OF DEATH: 04/19/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/19/00 7:41 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 706.9 GRAMS PROSECTOR: CHANESTA ACKER RECORDER: TERRI L. WILLIAMS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY LN, MEDIASTINAL (ML) : -LARGE; UP TO 11 X 10 X 5 MM; ALL APPEAR CYSTIC (CAP 23) FOOT/FOOTPAD (FP) : -CRUSTED AREA(S); RIGHT HINDFOOT: SINGLE DARK RED, DRY CRUSTED AREA, 2 MM; COLLECTED 3X GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN VAGINA (VA) : -HYPERPLASIA, MUCINOUS,-SLIGHT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MEDIASTINAL (ML) : -HEMORRHAGE,-PRESENT -LYMPHANGIECTASIS,-MODERATELY SEVERE FOOT/FOOTPAD (FP) : -INFLAMMATION, CHRONIC-ACTIVE,MODERATELY SEVERE -ULCERATION,-SLIGHT -ACANTHOSIS / HYPERKERATOSIS,-MODERATE -BACTERIA,-PRESENT 3812 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2051 ANIMAL NUMBER: C93161 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 706.9 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 7:41 PROSECTOR: CHANESTA ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), ADRENAL, MEDULLA (MA), OVARY (OV), BONE, FEMUR (FE), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), HEMATO NEOPLASIA (HN) 3813 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2052 ANIMAL NUMBER: C93162 SEX: FEMALE DATE OF DEATH: 04/19/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/19/00 10:14 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 402.8 GRAMS PROSECTOR: TONY HANFELD RECORDER: LAURIE J. SCHULLER PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MISSING; TEETH; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); INGUINAL REGION-LEFT; WEEK 102; 10 X 10MM; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-MODERATE -GRANULAR CYTOPLASM, EOSINOPHILIC, CENTRILOBULAR,-SLIGHT -PIGMENT, HEPATOCELLULAR, CENTRILOBULAR,-SLIGHT -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL -MINERALIZATION, TUBULAR,-MINIMAL -HYPERPLASIA, TRANSITIONAL CELL,MINIMAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SLIGHT 3814 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2053 ANIMAL NUMBER: C93162 SEX: FEMALE DATE OF DEATH: 04/19/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/19/00 10:14 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 402.8 GRAMS PROSECTOR: TONY HANFELD RECORDER: LAURIE J. SCHULLER PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY ADRENAL, CORTEX (AC) : -LARGE; LEFT: 5 MM IN DIAMETER (RIGHT IS 3 MM IN DIAMETER) OVARY (OV) : -CYST(S); BILATERAL: SINGLE CLEAR FLUID FILLED CYST ON EACH, LEFT IS 3 MM IN DIAMETER, RIGHT IS 2 MM IN DIAMETER LUNG (LU) : -LIGHT FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE TAN AREAS, UP TO 6 X 4 MM PITUITARY (PI) : -RED FOCUS(I)/AREA(S); POSTERIOR ASPECT: SINGLE DARK RED AREA, 3 X 2 MM PANCREAS (PA) : -B-ADENOMA, ISLET CELL,-NEOPLASM PRESENT -INFILTRATION, FAT, INTERSTITIAL,MINIMAL ADRENAL, CORTEX (AC) : -HYPERTROPHY, CORTICAL CELL,-MODERATE -DEGENERATION, CYSTIC / HEMORRHAGIC,MODERATE -PIGMENT,-MINIMAL OVARY (OV) : -CYST,-PRESENT -UNILATERALLY EXAMINED,-PRESENT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY) MODERATE LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,MODERATE HEART (HT) : -CARDIOMYOPATHY,-MINIMAL PITUITARY (PI) : -ANGIECTASIS,-PRESENT MAMMARY, FEMALE (MF) : -HYPERPLASIA, GLANDULAR,-MINIMAL 3815 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2054 ANIMAL NUMBER: C93162 SEX: FEMALE DATE OF DEATH: 04/19/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/19/00 10:14 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 402.8 GRAMS PROSECTOR: TONY HANFELD RECORDER: LAURIE J. SCHULLER PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS(ES); LEFT INGUINAL REGION: SINGLE MASS, 32 X 23 X 15 MM, TAN, RED, AND FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (INGUINAL) NORMAL (CAP 22) LN, ANT MES/PANC (AP) : -DIFFUSELY RED; DARK RED (CAP 24) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -MALOCCLUSION; BILATERAL LOWER INCISORS -TEETH MISSING/BROKEN/WORN; BILATERAL UPPER INCISORS: MISSING MAMMARY, FEMALE (MF) : -B-ADENOMA,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, INGUINAL (IN) : >TISSUE MISSING LN, ANT MES/PANC (AP) : >NOT REQUIRED TO BE EXAMINED FOR ANIMAL >NOTE:>SEE PANCREAS FOR DIAGNOSIS 3816 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2055 ANIMAL NUMBER: C93162 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 402.8 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 10:14 PROSECTOR: TONY HANFELD RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 3817 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2056 ANIMAL NUMBER: C93163 SEX: FEMALE DATE OF DEATH: 04/20/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/20/99 9:57 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 310.9 GRAMS PROSECTOR: KEVIN BILLINGS RECORDER: TONY HANFELD PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) LF ADRENAL (AD0) RT ADRENAL (AD1) BRAIN (BR) LF KIDNEY (KD0) RT KIDNEY (KD1) LIVER (LI) LUNG (LU) LF OVARY (OV0) RT OVARY (OV1) SPLEEN (SP) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) UTERUS (UT) .0311 .0283 2.1210 1.4342 1.4052 14.7661 1.4413 .0617 .0585 .4675 ..00100905 1.4084 .0100 % .0091 % .6822 % .4613 % .4520 % 4.7495 % .4636 % .0198 % .0188 % .1504 % .0031 % .0032 % .4530 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0147 .0133 1.0000 .6762 .6625 6.9619 .6795 .0291 .0276 .2204 .0045 .0047 .6640 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-SLIGHT -PIGMENT, HEPATOCELLULAR, CENTRILOBULAR,-MINIMAL -NECROSIS, INDIVIDUAL HEPATOCYTE,MINIMAL KIDNEY (KD) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -DILATATION, PELVIC,-MINIMAL 3818 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2057 ANIMAL NUMBER: C93163 SEX: FEMALE DATE OF DEATH: 04/20/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/20/99 9:57 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 310.9 GRAMS PROSECTOR: KEVIN BILLINGS RECORDER: TONY HANFELD PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY SPLEEN (SP) : -CYST(S); ALONG MARGIN OF CAUDAL END: SINGLE CLEAR FLUID FILLED CYST, 2 MM IN DIAMETER GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE -FIBROSIS, CAPSULAR,-PRESENT >NOTE:>FIBROUS TAGS POSSIBLY REMAINS OF CYST WALLS ADRENAL, CORTEX (AC) : -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL PITUITARY (PI) : -HYPERPLASIA,-SLIGHT MAMMARY, FEMALE (MF) : -HYPERPLASIA, GLANDULAR,-MINIMAL -SECRETORY MATERIAL,-PRESENT UTERUS (UT) : -DILATATION,-MODERATE CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 3819 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2058 ANIMAL NUMBER: C93163 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 310.9 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 9:57 PROSECTOR: KEVIN BILLINGS RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 3820 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2059 ANIMAL NUMBER: C93164 SEX: FEMALE DATE OF DEATH: 04/19/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/19/00 10:08 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 432.4 GRAMS PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); INGUINAL REGION-LEFT; WEEK 77; 65 X 40MM; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; BLUE SKIN; VENTRALABDOMINAL-LEFT; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -RAISED FOCUS(I)/AREA(S); DIAPHRAGMATIC SURFACE OF RIGHT MEDIAN LOBE: SINGLE DARK RED AREA, 2 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION HISTOPATHOLOGY BRAIN (BR) : >UNREMARKABLE LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-MODERATE -GRANULAR CYTOPLASM, EOSINOPHILIC, CENTRILOBULAR,-MODERATE -PIGMENT, HEPATOCELLULAR, CENTRILOBULAR,-SLIGHT -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL -CYST, BILIARY,-PRESENT -B-ADENOMA, HEPATOCELLULAR,-NEOPLASM PRESENT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, PELVIC,-SEVERE 3821 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2060 ANIMAL NUMBER: C93164 SEX: FEMALE DATE OF DEATH: 04/19/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/19/00 10:08 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 432.4 GRAMS PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 7 X 6 X 4 MM, DARK RED, RED AND SOFT SKIN (SK) : >NOTE:>ROUTINE SECTION COLLECTED FROM RIGHT INGUINAL REGION DUE TO MASS MAMMARY, FEMALE (MF) : -MASS(ES); LEFT INGUINAL REGION, EXTENDING INTO LEFT PERIANAL REGION: SINGLE MASS, 65 X 45 X 30 MM, RED, DARK RED, TAN, FIRM AND LOBULATED; SOME AREAS EXUDE A DARK BROWN SEMIFLUID; PORTION COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED KIDNEY (KD) : -HYPERPLASIA, TRANSITIONAL CELL,MODERATE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),SLIGHT LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-MODERATE -B-ADENOMA, C-CELL,-NEOPLASM PRESENT -B-ADENOMA, FOLLICULAR CELL,-NEOPLASM PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -M-CARCINOMA,-NEOPLASM PRESENT -CYST / GALACTOCELE,-PRESENT -B-FIBROADENOMA,-NEOPLASM PRESENT -HYPERPLASIA, GLANDULAR,-MINIMAL >NOTE:>FIBROADENOMA IS ON SLIDE 22; NOT NOTED MACROSCOPICALLY MASS 1 IS THE CARCINOMA 3822 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2061 ANIMAL NUMBER: C93164 SEX: FEMALE DATE OF DEATH: 04/19/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/19/00 10:08 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 432.4 GRAMS PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -CYST(S); RIGHT INGUINAL REGION: MULTIPLE TAN CREAMY SEMIFLUID FILLED CYSTS, UP TO 20 MM IN DIAMETER (CAP 22) >NOTE:>ROUTINE SECTION COLLECTED FROM RIGHT INGUINAL REGION DUE TO MASS UTERUS (UT) : -MASS(ES); WITHIN LUMEN OF MID LEFT HORN: SINGLE MASS, 3 MM IN DIAMETER, TAN AND FIRM; COLLECTED INTACT; REGIONAL LN (LUMBAR) NORMAL (CAP 24) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN UTERUS (UT) : -HYPERPLASIA, CYSTIC, ENDOMETRIAL,SLIGHT VAGINA (VA) : -HYPERPLASIA, MUCINOUS,-MODERATE CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, LUMBAR/ILIAC (LM) : -HYPERPLASIA, LYMPHOPLASMACYTIC,MODERATELY SEVERE 3823 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2062 ANIMAL NUMBER: C93164 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 432.4 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 10:08 PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), HEMATO NEOPLASIA (HN) 3824 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2063 ANIMAL NUMBER: C93165 SEX: FEMALE DATE OF DEATH: 04/21/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/21/00 9:53 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 424.3 GRAMS PROSECTOR: STEVE VAN ADESTINE RECORDER: LAURIE J. SCHULLER PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); VENTRAL-THORACIC; WEEK 93; 30 X 30MM; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; DORSALCERVICAL; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; DORSALCERVICAL-LEFT; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; DORSALCERVICAL-RIGHT; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -MOTTLED; ALL LOBES: RED AND TAN HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -MINERALIZATION,-MINIMAL LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-SLIGHT -GRANULAR CYTOPLASM, EOSINOPHILIC, CENTRILOBULAR,-SLIGHT -PIGMENT, HEPATOCELLULAR, CENTRILOBULAR,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MODERATE -DEGENERATION, CYSTIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-SLIGHT -B-ADENOMA, HEPATOCELLULAR,-NEOPLASM PRESENT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL 3825 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2064 ANIMAL NUMBER: C93165 SEX: FEMALE DATE OF DEATH: 04/21/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/21/00 9:53 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 424.3 GRAMS PROSECTOR: STEVE VAN ADESTINE RECORDER: LAURIE J. SCHULLER PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 7 X 6 X 5 MM, RED, DARK RED, GRAY, AND SOFT SKIN (SK) : -CRUSTED AREA(S); BILATERAL SHOULDER REGIONS, DIFFUSE: MULTIPLE BROWN DRY CRUSTED AREAS, UP TO 5 X 2 MM; CONSISTENT WITH ALL SCABS NOTED IN CLINICAL OBSERVATIONS (CAP 23) SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SLIGHT ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT -HYPERTROPHY, CORTICAL CELL,-MINIMAL -PIGMENT,-MINIMAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATE LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL -N-CARCINOMA,-FROM MAMMARY, FEMALE HEART (HT) : -CARDIOMYOPATHY,-MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PITUITARY (PI) : -ANGIECTASIS,-PRESENT SKIN (SK) : -ACANTHOSIS/HYPERKERATOSIS,-SLIGHT -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT -ULCERATION,-MINIMAL 3826 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2065 ANIMAL NUMBER: C93165 SEX: FEMALE DATE OF DEATH: 04/21/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/21/00 9:53 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 424.3 GRAMS PROSECTOR: STEVE VAN ADESTINE RECORDER: LAURIE J. SCHULLER PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS(ES); VENTRAL CERVICAL REGION: SINGLE MASS, 40 X 30 X 24 MM, DARK RED, TAN, GRAY, BROWN, AND LOBULATED; ON SECTION EXUDES A BROWN CREAMY SEMIFLUID; COLLECTED INTACT; REGIONAL LN (SEE MANDIBULAR) UTERUS (UT) : -CYST(S); BILATERAL, DIFFUSE: MULTIPLE CLEAR FLUID FILLED CYSTS, UP TO 4 MM IN DIAMETER CERVIX (CV) : -MASS(ES); INVOLVING ENTIRE ORGAN: SINGLE MASS, 30 X 23 X 20 MM, RED, DARK RED, TAN, GRAY, LOBULATED, SOFT, AND GELATINOUS; ON SECTION EXUDES A DARK RED FLUID; COLLECTED INTACT; REGIONAL LN (LUMBAR) NORMAL (CAP 24) LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL (CAP 22) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN MAMMARY, FEMALE (MF) : -M-CARCINOMA,-NEOPLASM PRESENT -CYST / GALACTOCELE,-PRESENT -HYPERPLASIA, GLANDULAR,-MINIMAL UTERUS (UT) : -HYPERPLASIA, CYSTIC, ENDOMETRIAL,MODERATELY SEVERE CERVIX (CV) : -M-SARCOMA, STROMAL,-NEOPLASM PRESENT -HEMORRHAGE,-PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MANDIBULAR (MN) : >UNREMARKABLE LN, LUMBAR/ILIAC (LM) : -HYPERPLASIA, LYMPHOPLASMACYTIC,MODERATE 3827 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2066 ANIMAL NUMBER: C93165 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 424.3 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 9:53 PROSECTOR: STEVE VAN ADESTINE RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), VAGINA (VA), HEMATO NEOPLASIA (HN) (SG), 3828 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2067 ANIMAL NUMBER: C93166 SEX: FEMALE DATE OF DEATH: 04/20/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/20/00 10:11 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 482.5 GRAMS PROSECTOR: GREGORY CODDINGTON RECORDER: TONE EVERTS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE DARK RED AREAS, PINPOINT AND UP TO 3 X 2 MM; COLLECTED ON ROUTINE SECTIONS -RAISED FOCUS(I)/AREA(S); ONE PAPILLARY PROCESS: SINGLE BROWN AREA, 6 X 6 X 1 MM (CAP 22) ADRENAL, CORTEX (AC) : -RED FOCUS(I)/AREAS(I); RIGHT: SINGLE DARK RED AREA, 3 MM IN DIAMETER HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-SLIGHT -VACUOLATION, HEPATOCELLULAR, MIDZONAL / CENTRILOBULAR,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-SLIGHT -FIBROSIS, PERIBILIARY,-SLIGHT -B-ADENOMA, HEPATOCELLULAR,-NEOPLASM PRESENT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, PELVIC,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SLIGHT ADRENAL, CORTEX (AC) : -HYPERTROPHY, CORTICAL CELL,-SLIGHT -DEGENERATION, CYSTIC / HEMORRHAGIC,SLIGHT -PIGMENT,-MINIMAL 3829 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2068 ANIMAL NUMBER: C93166 SEX: FEMALE DATE OF DEATH: 04/20/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/20/00 10:11 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 482.5 GRAMS PROSECTOR: GREGORY CODDINGTON RECORDER: TONE EVERTS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY OVARY (OV) : -CYST(S); BILATERAL: SINGLE CLEAR FLUID FILLED CYST ON EACH, RIGHT IS 3 MM IN DIAMETER, LEFT IS 9 MM IN DIAMETER LUNG (LU) : -LIGHT FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE WHITE AREAS, UP TO 12 X 10 MM SKIN (SK) : -MASS(ES); RIGHT INGUINAL REGION: SINGLE MASS, 12 X 12 X 8 MM, RED, TAN, AND FIRM; SAME ON CUT SURFACE (CAP 23); REGIONAL LN (INGUINAL) NOT IDENTIFIED OVARY (OV) : -CYST,-PRESENT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATE LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,SLIGHT -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL HEART (HT) : -CARDIOMYOPATHY,-MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-SLIGHT RECTUM (RE) : -PARASITISM,-PRESENT SKIN (SK) : -CYST, EPIDERMAL INCLUSION,-PRESENT MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT -HYPERPLASIA, GLANDULAR,-SLIGHT 3830 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2069 ANIMAL NUMBER: C93166 SEX: FEMALE DATE OF DEATH: 04/20/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/20/00 10:11 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 482.5 GRAMS PROSECTOR: GREGORY CODDINGTON RECORDER: TONE EVERTS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY UTERUS (UT) : -CYST(S); LEFT HORN, WITHIN WALLS, NEAR BIFURCATION: SINGLE CLEAR FLUID FILLED CYST, 13 X 5 X 5 MM GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN UTERUS (UT) : -B-POLYP, ENDOMETRIAL, STROMAL,NEOPLASM PRESENT VAGINA (VA) : -LUMEN, EXUDATE,-PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 3831 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2070 ANIMAL NUMBER: C93166 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 482.5 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 10:11 PROSECTOR: GREGORY CODDINGTON RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM COLON (CO), URINARY BLADDER (UB), CERVIX (CV), HEMATO NEOPLASIA (HN) (CE), 3832 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2071 ANIMAL NUMBER: C93167 SEX: FEMALE DATE OF DEATH: 01/12/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 01/12/98 14:30 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 268 STUDY WEEK OF DEATH: 39 TERMINAL BODY WEIGHT: 225.0 GRAMS PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: NOT OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; ROUGH HAIR COAT; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; YELLOW HAIR COAT; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; PALE; ENTIRE BODY; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -MOTTLED; ALL LOBES: TAN AND BROWN KIDNEY (KD) : -ROUGH SURFACE, DIFFUSE; BILATERAL SPLEEN (SP) : -SMALL; 25 X 8 X 6 MM HISTOPATHOLOGY LIVER (LI) : -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-SEVERE KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, PELVIC,-MINIMAL -VACUOLATION, TUBULAR EPITHELIUM,SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-MINIMAL PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,MODERATELY SEVERE MARROW, FEMUR (FM) : -HYPOCELLULAR, MARROW,-SLIGHT MARROW, STERNUM (SE) : -HYPOCELLULAR, MARROW,-SLIGHT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATELY SEVERE LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL 3833 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2072 ANIMAL NUMBER: C93167 SEX: FEMALE DATE OF DEATH: 01/12/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 01/12/98 14:30 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 268 STUDY WEEK OF DEATH: 39 TERMINAL BODY WEIGHT: 225.0 GRAMS PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA: MULTIPLE TAN ERODED AREAS, UP TO 3 X 2 MM GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -TEETH MISSING/BROKEN/WORN; MISSING, BILATERAL LOWER INCISORS -DISCHARGE-OCULAR; BILATERAL: DARK RED AND DRY -ANIMAL THIN -STAINS-PERINEUM/PERIANAL; YELLOW AND MOIST ESOPHAGUS (ES) : -HYPERKERATOSIS,-SLIGHT STOMACH, NONGL (SU) : -ACANTHOSIS / HYPERKERATOSIS,-SLIGHT -INFLAMMATION, SUBACUTE,-SLIGHT -ULCERATION,-MINIMAL VAGINA (VA) : -LUMEN, EXUDATE,-PRESENT CAUSE OF DEATH (CD) : -UNDETERMINED MICROSCOPICALLY,-PRESENT 3834 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2073 ANIMAL NUMBER: C93167 SEX: FEMALE DATE OF DEATH: 01/12/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 01/12/98 14:30 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 268 STUDY WEEK OF DEATH: 39 TERMINAL BODY WEIGHT: 225.0 GRAMS PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), HEMATO NEOPLASIA (HN) 3835 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2074 ANIMAL NUMBER: C93168 SEX: FEMALE DATE OF DEATH: 04/20/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/20/00 7:45 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 372.2 GRAMS PROSECTOR: TONY HANFELD RECORDER: JERALD W. JOHNSON PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-SLIGHT -GRANULAR CYTOPLASM, EOSINOPHILIC, CENTRILOBULAR,-SLIGHT -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, TUBULAR,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATELY SEVERE ADRENAL, CORTEX (AC) : -HYPERTROPHY, CORTICAL CELL,-MODERATE -DEGENERATION, CYSTIC / HEMORRHAGIC,MINIMAL -HYPERPLASIA, CORTICAL,-MINIMAL 3836 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2075 ANIMAL NUMBER: C93168 SEX: FEMALE DATE OF DEATH: 04/20/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/20/00 7:45 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 372.2 GRAMS PROSECTOR: TONY HANFELD RECORDER: JERALD W. JOHNSON PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY OVARY (OV) : -CYST(S); RIGHT: SINGLE CLEAR FLUID FILLED CYST, 4 MM IN DIAMETER LUNG (LU) : -LIGHT FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE TAN AREAS, UP TO 6 X 4 MM PITUITARY (PI) : -LARGE; 6 X 5 X 3 MM CAVITY, ABDOM (PC) : -MASS(ES); ATTACHED TO ADIPOSE TISSUE OF RIGHT OVARY: SINGLE MASS, 18 X 8 X 8 MM, TAN, GREEN AND FIRM; SAME ON CUT SURFACE (CAP 22); REGIONAL LN (LUMBAR) NORMAL (CAP 23) ADRENAL, CORTEX (AC) : -PIGMENT,-MINIMAL OVARY (OV) : -CYST,-PRESENT -UNILATERALLY EXAMINED,-PRESENT LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL -INFILTRATE, MACROPHAGE, ALVEOLAR,MODERATELY SEVERE HEART (HT) : -CARDIOMYOPATHY,-MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT -HYPERPLASIA, GLANDULAR,-SLIGHT UTERUS (UT) : -HYPERPLASIA, CYSTIC, ENDOMETRIAL,SLIGHT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT CAVITY, ABDOM (PC) : -INFLAMMATION / NECROSIS, FAT,-MODERATE 3837 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2076 ANIMAL NUMBER: C93168 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/20/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 372.2 GRAMS DATE AND TIME OF NECROPSY: 04/20/00 7:45 PROSECTOR: TONY HANFELD RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN), LN, LUMBAR/ILIAC (LM) 3838 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2077 ANIMAL NUMBER: C93169 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 232.8 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 10:51 PROSECTOR: LAURIE J. SCHULLER RECORDER: KIMBERLY DURLAND POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) LF ADRENAL (AD0) RT ADRENAL (AD1) BRAIN (BR) LF KIDNEY (KD0) RT KIDNEY (KD1) LIVER (LI) LUNG (LU) LF OVARY (OV0) RT OVARY (OV1) SPLEEN (SP) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) UTERUS (UT) .0281 .0250 1.9996 1.1705 1.1404 10.6066 1.0841 .0604 .0631 .4329 .0062 .0092 1.3993 .0121 % .0107 % .8589 % .5028 % .4899 % 4.5561 % .4657 % .0259 % .0271 % .1860 % .0027 % .0040 % .6011 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0141 .0125 1.0000 .5854 .5703 5.3044 .5422 .0302 .0316 .2165 .0031 .0046 .6998 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: SINGLE DARK BROWN FOCUS, 2 MM IN DIAMETER LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-SLIGHT -NECROSIS, INDIVIDUAL HEPATOCYTE,MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE STOMACH, GL (ST) : >NOT REQUIRED TO BE EXAMINED FOR ANIMAL 3839 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2078 ANIMAL NUMBER: C93169 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/21/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 232.8 GRAMS DATE AND TIME OF NECROPSY: 07/21/98 10:51 PROSECTOR: LAURIE J. SCHULLER RECORDER: KIMBERLY DURLAND POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JILL PAUS LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY UTERUS (UT) : -LUMEN FILLED WITH FLUID; BILATERAL: CLEAR FLUID GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN UTERUS (UT) : >NOT REQUIRED TO BE EXAMINED FOR ANIMAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV) 3840 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2079 ANIMAL NUMBER: C93170 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/01/00 STUDY DAY OF DEATH: 653 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: 340.0 GRAMS DATE AND TIME OF NECROPSY: 02/01/00 13:33 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; HEAD TILT; RIGHT; VERIFIED AT NECROPSY: NOT APPLICABLE >EYES; RED DISCHARGE; EYE-LEFT; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; ATAXIC; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -DIFFUSELY DARK; ALL LOBES: DARK BROWN -MASS(ES); ONE PAPILLARY PROCESS, INVOLVING ENTIRE LOBE: SINGLE MASS, 20 X 15 X 10 MM, RED, DARK RED, BROWN, VASCULAR AND FIRM; SAME ON CUT SURFACE (CAP 21); REGIONAL LN (HEPATIC) NORMAL (CAP 22) OVARY (OV) : -CYST(S); RIGHT: SINGLE CLEAR FLUID FILLED CYST, 10 MM IN DIAMETER HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-SLIGHT -NECROSIS, INDIVIDUAL HEPATOCYTE,MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL -M-CARCINOMA, HEPATOCELLULAR,-NEOPLASM PRESENT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : -PIGMENT,-MINIMAL OVARY (OV) : -CYST,-PRESENT 3841 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2080 ANIMAL NUMBER: C93170 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/01/00 STUDY DAY OF DEATH: 653 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: 340.0 GRAMS DATE AND TIME OF NECROPSY: 02/01/00 13:33 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY LUNG (LU) : -LIGHT FOCUS(I)/AREA(S); RIGHT CAUDAL LOBE: MULTIPLE TAN AREAS, UP TO 10 X 5 MM PITUITARY (PI) : -MASS(ES); 13 X 7 X 6 MM, RED, DARK RED, TAN, GRAY AND SOFT MARROW, FEMUR (FM) : -HYPOCELLULAR, MARROW,-MODERATELY SEVERE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),SLIGHT LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,SLIGHT HEART (HT) : -CARDIOMYOPATHY,-MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -B-FIBROADENOMA,-NEOPLASM PRESENT >NOTE:>SEE SLIDE #19 VAGINA (VA) : -LUMEN, EXUDATE,-PRESENT -HYPERPLASIA, MUCINOUS,-MODERATE CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT LN, OTHER (LN) : -INFILTRATE, MACROPHAGE, PIGMENTED,MODERATE 3842 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2081 ANIMAL NUMBER: C93170 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/01/00 STUDY DAY OF DEATH: 653 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: 340.0 GRAMS DATE AND TIME OF NECROPSY: 02/01/00 13:33 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: RED AND DRY THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), HEMATO NEOPLASIA (HN) 3843 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2082 ANIMAL NUMBER: C93171 SEX: FEMALE DATE OF DEATH: 04/20/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/20/99 8:35 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 350.6 GRAMS PROSECTOR: JOHN S. HALFORD RECORDER: TONY HANFELD PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) LF ADRENAL (AD0) RT ADRENAL (AD1) BRAIN (BR) LF KIDNEY (KD0) RT KIDNEY (KD1) LIVER (LI) LUNG (LU) LF OVARY (OV0) RT OVARY (OV1) SPLEEN (SP) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) UTERUS (UT) .0262 .0341 2.1395 1.1620 1.1783 13.5288 1.4643 .0765 .0678 .4089 .0117 .0214 1.2207 .0075 % .0097 % .6102 % .3314 % .3361 % 3.8588 % .4177 % .0218 % .0193 % .1166 % .0033 % .0061 % .3482 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0122 .0159 1.0000 .5431 .5507 6.3233 .6844 .0358 .0317 .1911 .0055 .0100 .5706 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-MINIMAL -PIGMENT, HEPATOCELLULAR, CENTRILOBULAR,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : -HYPERPLASIA, CORTICAL,-MINIMAL 3844 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2083 ANIMAL NUMBER: C93171 SEX: FEMALE DATE OF DEATH: 04/20/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/20/99 8:35 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 350.6 GRAMS PROSECTOR: JOHN S. HALFORD RECORDER: TONY HANFELD PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY LUNG (LU) : -LIGHT FOCUS(I)/AREA(S); RIGHT CAUDAL LOBE: TWO TAN AREAS, ONE IS 3 X 2 MM, THE OTHER IS 2 X 1 MM PITUITARY (PI) : -LARGE; 5 X 4 X 4 MM; APPEARS SWOLLEN UTERUS (UT) : -LUMEN FILLED WITH FLUID; BILATERAL: CLEAR FLUID GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN THYMUS (TH) : -HEMORRHAGE,-PRESENT LUNG (LU) : -INFILTRATE, MACROPHAGE, SLIGHT ALVEOLAR,- PITUITARY (PI) : >UNREMARKABLE MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT UTERUS (UT) : -DILATATION,-MINIMAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 3845 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2084 ANIMAL NUMBER: C93171 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/20/99 STUDY DAY OF DEATH: 366 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 350.6 GRAMS DATE AND TIME OF NECROPSY: 04/20/99 8:35 PROSECTOR: JOHN S. HALFORD RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: JARED FISHEL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 3846 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2085 ANIMAL NUMBER: C93172 SEX: FEMALE DATE OF DEATH: 10/17/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 10/17/99 14:31 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 546 STUDY WEEK OF DEATH: 78 TERMINAL BODY WEIGHT: 212.6 GRAMS PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >EYES; SQUINTED; PERI-ORBITAL-RIGHT; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; HEAD TILT; LEFT; VERIFIED AT NECROPSY: NOT APPLICABLE >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; HUNCHED; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; ATAXIC; VERIFIED AT NECROPSY: NOT APPLICABLE >EYES; RED DISCHARGE; EYE-RIGHT; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -LIGHT FOCUS(I)/AREA(S); VISCERAL SURFACE OF LEFT LATERAL LOBE: SINGLE TAN FOCUS, 1 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION THYMUS (TH) : -MISSING/NOT IDENTIFIED; NOT POSITIVELY IDENTIFIED; POSSIBLE REMNANTS COLLECTED HISTOPATHOLOGY BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-MINIMAL -INFILTRATE, MACROPHAGE, PIGMENTED,SLIGHT -LIPIDOSIS, SUBCAPSULAR (TENSION LIPIDOSIS),-MINIMAL KIDNEY (KD) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -MINERALIZATION, PELVIC,-MINIMAL -MINERALIZATION, TUBULAR,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT -PIGMENT,-MINIMAL THYMUS (TH) : >SECTION EXAMINED; TISSUE NOT PRESENT 3847 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2086 ANIMAL NUMBER: C93172 SEX: FEMALE DATE OF DEATH: 10/17/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 10/17/99 14:31 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 546 STUDY WEEK OF DEATH: 78 TERMINAL BODY WEIGHT: 212.6 GRAMS PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY ESOPHAGUS (ES) : -MASS(ES); 11 MM FROM EPIGLOTTIS, ATTACHED TO WALL WITHIN LUMEN: SINGLE TAN AND FIRM MASS, 2 X 2 X 1 MM; CUT SURFACE HAS A FIRM PLASTIC-LIKE AREA, 1 MM IN DIAMETER; COLLECTED INTACT; REGIONAL LN (MEDIASTINAL) NORMAL (CAP 21) PITUITARY (PI) : -MASS(ES); 14 X 13 X 10 MM, RED, DARK RED, GREY, LOBULATED AND SOFT GENERAL COMMENT (GC) -BLOOD FILM TAKEN -DISCHARGE-OCULAR; MATERIAL -ANIMAL THIN : RIGHT: DRY, RED LUNG (LU) : -INFILTRATE, MACROPHAGE, MINIMAL ESOPHAGUS (ES) : >UNREMARKABLE ALVEOLAR,- PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT RECTUM (RE) : -PARASITISM,-PRESENT MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT VAGINA (VA) : -LUMEN, EXUDATE,-PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT LN, MEDIASTINAL (ML) : >TISSUE MISSING 3848 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2087 ANIMAL NUMBER: C93172 SEX: FEMALE DATE OF DEATH: 10/17/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 10/17/99 14:31 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 546 STUDY WEEK OF DEATH: 78 TERMINAL BODY WEIGHT: 212.6 GRAMS PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), HEMATO NEOPLASIA (HN) 3849 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2088 ANIMAL NUMBER: C93173 SEX: FEMALE DATE OF DEATH: 03/28/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 03/28/00 11:17 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 709 STUDY WEEK OF DEATH: 102 TERMINAL BODY WEIGHT: 394.3 GRAMS PROSECTOR: JERALD W. JOHNSON RECORDER: JERALD W. JOHNSON PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); SHOULDER-RIGHT; WEEK 88; 20 X 30MM; VERIFIED AT NECROPSY: OBSERVED >DISCHARGE; NASAL; RED IN COLOR; VERIFIED AT NECROPSY: OBSERVED >EYES; RED DISCHARGE; EYES; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -DIFFUSELY LIGHT; ALL LOBES: TAN HISTOPATHOLOGY BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-SLIGHT -GRANULAR CYTOPLASM, EOSINOPHILIC, CENTRILOBULAR,-MINIMAL -PIGMENT, HEPATOCELLULAR, CENTRILOBULAR,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-MODERATELY SEVERE -HYPERPLASIA, BILE DUCT,-MINIMAL -FIBROSIS, PERIBILIARY,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, PELVIC,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-MODERATE 3850 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2089 ANIMAL NUMBER: C93173 SEX: FEMALE DATE OF DEATH: 03/28/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 03/28/00 11:17 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 709 STUDY WEEK OF DEATH: 102 TERMINAL BODY WEIGHT: 394.3 GRAMS PROSECTOR: JERALD W. JOHNSON RECORDER: JERALD W. JOHNSON PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY ADRENAL, CORTEX (AC) : -DIFFUSELY RED; LEFT: DARK RED -SMALL; RIGHT: 3 X 3 X 2 MM (LEFT IS 5 X 4 X 3 MM) LUNG (LU) : -LIGHT FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE TAN AREAS, UP TO 6 MM IN DIAMETER THYROID (TY) : -LARGE; RIGHT: 4 X 3 X 3 MM (LEFT IS 3 X 2 X 1 MM) PITUITARY (PI) : -MASS(ES); 7 MM IN DIAMETER, BROWN, RED, TAN, VASCULAR AND SOFT STOMACH, GL (ST) : -RED FOCUS(I)/AREA(S); MUCOSA: MULTIPLE DARK RED AREAS, UP TO 3 X 1 MM PANCREAS (PA) : -INFILTRATION, FAT, INTERSTITIAL,MODERATE ADRENAL, CORTEX (AC) : -HYPERTROPHY, CORTICAL CELL,-MODERATE -DEGENERATION, CYSTIC / HEMORRHAGIC,MODERATE -PIGMENT,-SLIGHT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY) MODERATE LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,SLIGHT HEART (HT) : -CARDIOMYOPATHY,-MINIMAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : >UNREMARKABLE 3851 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2090 ANIMAL NUMBER: C93173 SEX: FEMALE DATE OF DEATH: 03/28/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 03/28/00 11:17 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 709 STUDY WEEK OF DEATH: 102 TERMINAL BODY WEIGHT: 394.3 GRAMS PROSECTOR: JERALD W. JOHNSON RECORDER: JERALD W. JOHNSON PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT LATERAL CERVICAL REGION: SINGLE MASS, 30 X 20 X 20 MM, DARK RED, RED, TAN, VASCULAR, LOBULATED AND FIRM; SAME ON CUT SURFACE; SOME SECTIONS EXUDE A DARK RED FLUID; COLLECTED; REGIONAL LN (MANDIBULAR) NORMAL (CAP 21) -THICKENED; ALL REGIONS: DIFFUSE; COLLECTED ON ROUTINE SECTION -CYST(S); ALL REGIONS, DIFFUSE: MULTIPLE WHITE SEMIFLUID FILLED CYSTS UP TO 2 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION VAGINA (VA) : -SEMIFLUID MATERIAL; WITHIN LUMEN: CONTAINS A LIGHT GREEN SEMIFLUID MATERIAL GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DARK RED, DRY MATERIAL -DISCHARGE-NASAL; DARK RED, DRY MATERIAL MAMMARY, FEMALE (MF) : -M-CARCINOMA,-NEOPLASM PRESENT -CYST / GALACTOCELE,-PRESENT VAGINA (VA) : -LUMEN, EXUDATE,-PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT LN, MANDIBULAR (MN) : -HYPERPLASIA, LYMPHOPLASMACYTIC,MODERATE 3852 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2091 ANIMAL NUMBER: C93173 SEX: FEMALE DATE OF DEATH: 03/28/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 03/28/00 11:17 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 709 STUDY WEEK OF DEATH: 102 TERMINAL BODY WEIGHT: 394.3 GRAMS PROSECTOR: JERALD W. JOHNSON RECORDER: JERALD W. JOHNSON PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), HEMATO NEOPLASIA (HN) 3853 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2092 ANIMAL NUMBER: C93174 SEX: FEMALE DATE OF DEATH: 04/19/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/19/00 11:17 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 458.2 GRAMS PROSECTOR: GREGORY CODDINGTON RECORDER: TERRI L. WILLIAMS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); VENTRAL-THORACIC; WEEK 100; 30 X 20 MM; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) HISTOPATHOLOGY BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-SLIGHT -GRANULAR CYTOPLASM, EOSINOPHILIC, CENTRILOBULAR,-MODERATE -PIGMENT, HEPATOCELLULAR, CENTRILOBULAR,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, PELVIC,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT -PIGMENT,-MINIMAL ADRENAL, MEDULLA (MA) : -B-PHEOCHROMOCYTOMA,-NEOPLASM PRESENT 3854 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2093 ANIMAL NUMBER: C93174 SEX: FEMALE DATE OF DEATH: 04/19/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/19/00 11:17 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 458.2 GRAMS PROSECTOR: GREGORY CODDINGTON RECORDER: TERRI L. WILLIAMS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 7 X 6 X 5 MM, RED, DARK RED AND SOFT MAMMARY, FEMALE (MF) : -MASS(ES); VENTRAL THORACIC REGION: SINGLE MASS, 45 X 30 X 15 MM, TAN AND SOFT; SAME ON CUT SURFACE; (CAP 22); REGIONAL LN (AXILLARY) NORMAL (CAP 23) -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION CERVIX (CV) : -LARGE; 8 MM IN DIAMETER GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-SLIGHT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT SKIN (SK) : -B-LIPOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -HYPERPLASIA, GLANDULAR,-MODERATELY SEVERE >NOTE:>SEE ALSO SKIN CERVIX (CV) : -HYPERPLASIA, MUCINOUS,-SEVERE VAGINA (VA) : -HYPERPLASIA, MUCINOUS,-SEVERE CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 3855 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2094 ANIMAL NUMBER: C93174 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/19/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 458.2 GRAMS DATE AND TIME OF NECROPSY: 04/19/00 11:17 PROSECTOR: GREGORY CODDINGTON RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUm /s KULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), HEMATO NEOPLASIA (HN), LN, AXILLARY (AX) (SE), 3856 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2095 ANIMAL NUMBER: C93175 SEX: FEMALE DATE OF DEATH: 01/20/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 01/20/00 10:20 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 641 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: 195.5 GRAMS PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK 75; 60 X 50MM; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; TREMORS; ENTIRE BODY; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; COLD TO TOUCH; ENTIRE BODY; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -DIFFUSELY DARK; ALL LOBES: DARK BROWN SPLEEN (SP) : -SMALL; 24 X 5 X 3 MM HISTOPATHOLOGY EYE (EY) : -INFLAMMATION, ACUTE,-MODERATE -ULCERATION, CORNEAL,-MODERATE BRAIN (BR) : >UNREMARKABLE LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-SLIGHT -GRANULAR CYTOPLASM, EOSINOPHILIC, CENTRILOBULAR,-MODERATE -PIGMENT, HEPATOCELLULAR, CENTRILOBULAR,-SLIGHT -HYPERPLASIA, BILE DUCT,-SLIGHT -FIBROSIS, PERIBILIARY,-SLIGHT -INFILTRATE, MACROPHAGE, PIGMENTED,SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SLIGHT -MINERALIZATION, TUBULAR,-MODERATE SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE 3857 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2096 ANIMAL NUMBER: C93175 SEX: FEMALE DATE OF DEATH: 01/20/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 01/20/00 10:20 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 641 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: 195.5 GRAMS PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY LUNG (LU) : -LIGHT FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE TAN AREAS, UP TO 6 X 4 MM PITUITARY (PI) : -MASS(ES); 5 MM IN DIAMETER, DARK RED, TAN AND FIRM PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-MODERATE ADRENAL, CORTEX (AC) : -HYPERTROPHY, CORTICAL CELL,-MODERATE -DEGENERATION, CYSTIC / HEMORRHAGIC,MODERATE ADRENAL, MEDULLA (MA) : -UNILATERALLY EXAMINED,-PRESENT MARROW, FEMUR (FM) : -HYPOCELLULAR, MARROW,-MODERATE -CONGESTION,-PRESENT MARROW, STERNUM (SE) : -HYPOCELLULAR, MARROW,-MODERATE -CONGESTION,-PRESENT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATELY SEVERE LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,MODERATE HEART (HT) : -CARDIOMYOPATHY,-MODERATE -MINERALIZATION, VASCULAR,-MODERATE -MINERALIZATION,-MODERATE PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT 3858 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2097 ANIMAL NUMBER: C93175 SEX: FEMALE DATE OF DEATH: 01/20/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 01/20/00 10:20 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 641 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: 195.5 GRAMS PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS(ES); LEFT INGUINAL REGION: SINGLE MASS, 60 X 39 X 15 MM, DARK RED, TAN, LOBULATED AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (INGUINAL) NOT IDENTIFIED VAGINA (VA) : -SEMIFLUID MATERIAL; WITHIN LUMEN: LIGHT GREEN MUCOID MATERIAL GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; DRY RED MATERIAL -ANIMAL THIN -GI TRACT-DARK FLUID CONTENT; ALONG ENTIRE LENGTH: DARK BROWN MATERIAL MAMMARY, FEMALE (MF) : -M-CARCINOMA,-NEOPLASM PRESENT -CYST / GALACTOCELE,-PRESENT -HYPERPLASIA, GLANDULAR,-SLIGHT VAGINA (VA) : >UNREMARKABLE CAUSE OF DEATH (CD) : -NEOPLASM, MAMMARY GLAND,-PRESENT 3859 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2098 ANIMAL NUMBER: C93175 SEX: FEMALE DATE OF DEATH: 01/20/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 01/20/00 10:20 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 641 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: 195.5 GRAMS PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), HEMATO NEOPLASIA (HN) 3860 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2099 ANIMAL NUMBER: C93176 SEX: FEMALE DATE OF DEATH: 12/23/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 12/23/99 9:51 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 613 STUDY WEEK OF DEATH: 88 TERMINAL BODY WEIGHT: 258.1 GRAMS PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); INGUINAL REGION-LEFT; WEEK 72; 30 X 20MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); INGUINAL REGION-RIGHT; WEEK 79; 10 X 10MM; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; BLACK SKIN; VENTRALABDOMINAL-LEFT; VERIFIED AT NECROPSY: NOT OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; HUNCHED; VERIFIED AT NECROPSY: NOT APPLICABLE >EYES; RED DISCHARGE; EYES; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; ROUGH HAIR COAT; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >EXCRETION; NO FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; RECUMBENT; LATERAL; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) HISTOPATHOLOGY BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-SLIGHT -GRANULAR CYTOPLASM, EOSINOPHILIC, CENTRILOBULAR,-SLIGHT -PIGMENT, HEPATOCELLULAR, CENTRILOBULAR,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -DEGENERATION, CYSTIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-SLIGHT -FIBROSIS, PERIBILIARY,-SLIGHT KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL -INFLAMMATION, SUPPURATIVE, PELVIC,MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT 3861 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2100 ANIMAL NUMBER: C93176 SEX: FEMALE DATE OF DEATH: 12/23/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 12/23/99 9:51 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 613 STUDY WEEK OF DEATH: 88 TERMINAL BODY WEIGHT: 258.1 GRAMS PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 12 X 10 X 8 MM, DARK RED, TAN, AND SOFT STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA: MULTIPLE TAN ERODED AREAS, UP TO 5 X 2 MM ADRENAL, CORTEX (AC) : -HYPERPLASIA, CORTICAL,-MINIMAL -PIGMENT,-SLIGHT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),SLIGHT -CYST,-PRESENT LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,SLIGHT THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : -EDEMA,-MODERATE -INFLAMMATION, ACUTE,-SLIGHT STOMACH, NONGL (SU) : -ACANTHOSIS / HYPERKERATOSIS,-MODERATE -ULCERATION,-MODERATE -INFLAMMATION, CHRONIC-ACTIVE,MODERATELY SEVERE MAMMARY, FEMALE (MF) : -M-CARCINOMA,-NEOPLASM PRESENT -CYST / GALACTOCELE,-PRESENT -HYPERPLASIA, GLANDULAR,-MINIMAL -B-ADENOMA,-NEOPLASM PRESENT 3862 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2101 ANIMAL NUMBER: C93176 SEX: FEMALE DATE OF DEATH: 12/23/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 12/23/99 9:51 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 613 STUDY WEEK OF DEATH: 88 TERMINAL BODY WEIGHT: 258.1 GRAMS PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS(ES); LEFT PERINEAL REGION: SINGLE MASS, 25 X 25 X 15 MM, DARK RED, TAN, AND SOFT; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED -MASS #2; RIGHT INGUINAL REGION: SINGLE MASS, 15 X 15 X 10 MM, RED, DARK RED, TAN, AND SOFT; CUT SURFACE CONTAINS A DARK RED SEMISOLID; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DARK RED AND DRY -ANIMAL THIN -DISCHARGE-NASAL; DARK RED AND DRY MAMMARY, FEMALE (MF) : >NOTE:>MASS #1 IS THE CARCINOMA UTERUS (UT) : -DILATATION,-SLIGHT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 3863 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2102 ANIMAL NUMBER: C93176 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/23/99 STUDY DAY OF DEATH: 613 STUDY WEEK OF DEATH: 88 TERMINAL BODY WEIGHT: 258.1 GRAMS DATE AND TIME OF NECROPSY: 12/23/99 9:51 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 3864 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2103 ANIMAL NUMBER: C93177 SEX: FEMALE DATE OF DEATH: 04/21/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/21/00 11:28 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 328.2 GRAMS PROSECTOR: JOHN S. HALFORD RECORDER: LAURIE J. SCHULLER PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -RED FOCUS(I)/AREA(S); DIAPHRAGMATIC SURFACE OF RIGHT MEDIAN LOBE: MULTIPLE DARK RED AREAS, UP TO 4 X 3 MM; COLLECTED ON ROUTINE SECTION HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-MINIMAL -GRANULAR CYTOPLASM, EOSINOPHILIC, CENTRILOBULAR,-SLIGHT -PIGMENT, HEPATOCELLULAR, CENTRILOBULAR,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MODERATELY SEVERE -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL -FIBROSIS, PERIBILIARY,-MINIMAL -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, PELVIC,-SLIGHT -HYPERPLASIA, TRANSITIONAL CELL,-SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE 3865 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2104 ANIMAL NUMBER: C93177 SEX: FEMALE DATE OF DEATH: 04/21/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/21/00 11:28 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 328.2 GRAMS PROSECTOR: JOHN S. HALFORD RECORDER: LAURIE J. SCHULLER PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY ADRENAL, CORTEX (AC) : -LARGE; RIGHT: 5 MM IN DIAMETER (LEFT IS 3 MM IN DIAMETER) PITUITARY (PI) : -DARK FOCUS(I)/AREA(S); DIFFUSE: MULTIPLE BLACK AREAS, UP TO 3 X 2 MM GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -MALOCCLUSION; BILATERAL UPPER INCISORS ADRENAL, CORTEX (AC) : -HYPERTROPHY, CORTICAL CELL,-MODERATELY SEVERE -DEGENERATION, CYSTIC / HEMORRHAGIC,MODERATE -PIGMENT,-MINIMAL OVARY (OV) : -HYPERPLASIA, GRANULOSA CELL,-MODERATE PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -HYPERPLASIA, GLANDULAR,-MINIMAL URINARY BLADDER (UB) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL UTERUS (UT) : -DILATATION,-SLIGHT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 3866 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2105 ANIMAL NUMBER: C93177 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 328.2 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 11:28 PROSECTOR: JOHN S. HALFORD RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 3867 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2106 ANIMAL NUMBER: C93178 SEX: FEMALE DATE OF DEATH: 04/21/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/21/00 7:49 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 365.0 GRAMS PROSECTOR: STEVE VAN ADESTINE RECORDER: LAURIE J. SCHULLER PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >EYES; RED DISCHARGE; EYE-LEFT; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY SPLEEN (SP) : -SMALL; 34 X 6 X 4 MM HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-SLIGHT -GRANULAR CYTOPLASM, EOSINOPHILIC, CENTRILOBULAR,-MODERATE -PIGMENT, HEPATOCELLULAR, CENTRILOBULAR,-MINIMAL -HYPERPLASIA, BILE DUCT,-SLIGHT -FIBROSIS, PERIBILIARY,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, PELVIC,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-SLIGHT ADRENAL, CORTEX (AC) : -PIGMENT,-MINIMAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),SLIGHT 3868 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2107 ANIMAL NUMBER: C93178 SEX: FEMALE DATE OF DEATH: 04/21/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/21/00 7:49 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 365.0 GRAMS PROSECTOR: STEVE VAN ADESTINE RECORDER: LAURIE J. SCHULLER PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -LARGE; 5 X 6 X 7 MM, APPEARS SWOLLEN -DARK FOCUS(I)/AREA(S); ANTERIOR ASPECT: MULTIPLE BLACK AREAS, UP TO 3 X 1 MM MAMMARY, FEMALE (MF) : -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -DISCHARGE-OCULAR; LEFT: DRY, CRUSTED MATERIAL DARK RED, LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -HYPERPLASIA, GLANDULAR,-MODERATE CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 3869 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2108 ANIMAL NUMBER: C93178 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 365.0 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 7:49 PROSECTOR: STEVE VAN ADESTINE RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 3870 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2109 ANIMAL NUMBER: C93179 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 297.5 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 10:16 PROSECTOR: TROY ELLENBECKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); AXILLARY REGION-LEFT; WEEK 95; 40 X 40MM; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; AXILLARY REGION-LEFT; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -LIGHT FOCUS(I)/AREA(S); JUNCTION OF MEDIAN LOBE: SINGLE TAN AREA, 3 X 2 MM (CAP 22) HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-SLIGHT -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-SLIGHT -NECROSIS, INDIVIDUAL HEPATOCYTE,MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-SLIGHT -HYPERPLASIA, BILE DUCT,-SLIGHT -INFILTRATE, MACROPHAGE, PIGMENTED,SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, PELVIC,-SLIGHT SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : -PIGMENT,-MINIMAL 3871 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2110 ANIMAL NUMBER: C93179 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 297.5 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 10:16 PROSECTOR: TROY ELLENBECKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY ADRENAL, MEDULLA (MA) : -UNILATERALLY EXAMINED,-PRESENT OVARY (OV) : -HYPERPLASIA, GRANULOSA CELL,-SEVERE MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,-MODERATE MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,-MODERATE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATELY SEVERE LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-SLIGHT -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : -HYPERPLASIA,-MODERATE RECTUM (RE) : -PARASITISM,-PRESENT MAMMARY, FEMALE (MF) : -M-CARCINOMA,-NEOPLASM PRESENT -HYPERPLASIA, GLANDULAR,-SLIGHT 3872 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2111 ANIMAL NUMBER: C93179 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 297.5 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 10:16 PROSECTOR: TROY ELLENBECKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS(ES); LEFT AXILLARY REGION: SINGLE MASS, 40 X 25 X 20 MM, RED, TAN, AND FIRM; SAME ON CUT SURFACE; SOME SECTIONS EXUDE A TAN CASEOUS MATERIAL; OVERLYING SKIN HAS A SINGLE MOIST TAN AND ERODED AREA, 8 MM IN DIAMETER; COLLECTED INTACT WITH MASS; REGIONAL LN (SEE AXILLARY) UTERUS (UT) : -CYST(S); LEFT, WITHIN WALL: SINGLE CLEAR FLUID FILLED CYST, 10 X 5 X 5 MM LN, AXILLARY (AX) : -LARGE; LEFT: 10 X 7 X 2 MM 5 X 2 X 1 MM) (CAP 23) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN (RIGHT IS UTERUS (UT) : -HYPERPLASIA, CYSTIC, ENDOMETRIAL,MINIMAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, AXILLARY (AX) : -HYPERPLASIA, LYMPHOPLASMACYTIC,MODERATELY SEVERE 3873 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2112 ANIMAL NUMBER: C93179 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/21/00 STUDY DAY OF DEATH: 733 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 297.5 GRAMS DATE AND TIME OF NECROPSY: 04/21/00 10:16 PROSECTOR: TROY ELLENBECKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUm /s KULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), LN, MESENTERIC (MS), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 3874 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2113 ANIMAL NUMBER: C93180 SEX: FEMALE DATE OF DEATH: 06/28/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 06/28/99 14:15 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 435 STUDY WEEK OF DEATH: 63 TERMINAL BODY WEIGHT: 578.3 GRAMS PROSECTOR: KEVIN BILLINGS RECORDER: KEVIN BILLINGS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); VENTRAL-THORACIC-LEFT; WEEK 56; 110 X 80MM; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -LARGE; ALL LOBES: THICKENED HISTOPATHOLOGY BRAIN (BR) : ^UNREMARKABLE LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-MINIMAL -PIGMENT, HEPATOCELLULAR, CENTRILOBULAR,-SLIGHT -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL -CYST, BILIARY,-PRESENT KIDNEY (KD) : -MINERALIZATION, TUBULAR,-MINIMAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SLIGHT ADRENAL, CORTEX (AC) : -PIGMENT,-MINIMAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),SLIGHT 3875 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2114 ANIMAL NUMBER: C93180 SEX: FEMALE DATE OF DEATH: 06/28/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 06/28/99 14:15 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 435 STUDY WEEK OF DEATH: 63 TERMINAL BODY WEIGHT: 578.3 GRAMS PROSECTOR: KEVIN BILLINGS RECORDER: KEVIN BILLINGS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY LUNG (LU) : -LIGHT FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE LIGHT AREAS, UP TO 4 X 3 MM PITUITARY (PI) : -LARGE; 7 X 7 X 5 MM; APPEARS SWOLLEN -RED FOCUS(I)/AREA(S); RIGHT ASPECT: SINGLE DARK RED AREA, 2 MM IN DIAMETER MAMMARY, FEMALE (MF) : -MASS(ES); LEFT AXILLARY, EXTENDING TO LEFT ABDOMINAL REGION: SINGLE MASS, 130 X 80 X 70 MM (277.8 GRAMS), RED, TAN AND FIRM; SAME ON CUT SURFACE; PORTION COLLECTED; REGIONAL LN (AXILLARY) NOT IDENTIFIED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,SLIGHT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT SKIN (SK) : -M-FIBROSARCOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT >NOTE:>SEE SKIN FOR DIAGNOSIS CAUSE OF DEATH (CD) : -NEOPLASM, SKIN/MUSCLE/SUBCUTIS,PRESENT 3876 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2115 ANIMAL NUMBER: C93180 SEX: FEMALE DATE OF DEATH: 06/28/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 06/28/99 14:15 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 435 STUDY WEEK OF DEATH: 63 TERMINAL BODY WEIGHT: 578.3 GRAMS PROSECTOR: KEVIN BILLINGS RECORDER: KEVIN BILLINGS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) (SM), (CE), 3877 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2116 ANIMAL NUMBER: C93181 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 04/24/00 STUDY DAY OF DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 437.9 GRAMS DATE AND TIME OF NECROPSY: 04/24/00 10:50 PROSECTOR: JOHN S. HALFORD RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY OVARY (OV) : -CYST(S); RIGHT: SINGLE CLEAR FLUID FILLED CYST, 3 MM IN DIAMETER CERVIX (CV) : -MASS(ES); WITHIN LUMEN, EXTENDING INTO VAGINA: SINGLE MASS, 9 X 5 X 5 MM, DARK RED, LOBULATED AND SOFT; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (LUMBAR) NORMAL (CAP 22) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, TUBULAR,-MINIMAL OVARY (OV) : >UNREMARKABLE THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -HYPERPLASIA, GLANDULAR,-MINIMAL CERVIX (CV) : -B-POLYP, STROMAL,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 3878 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2117 ANIMAL NUMBER: C93181 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 04/24/00 STUDY DAY OF DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 437.9 GRAMS DATE AND TIME OF NECROPSY: 04/24/00 10:50 PROSECTOR: JOHN S. HALFORD RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN -MALOCCLUSION; BILATERAL: UPPER INCISORS THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), LUNG (LU), PARATHYROID (PT), URINARY BLADDER (UB), LN, LUMBAR/ILIAC (LM) 3879 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2118 ANIMAL NUMBER: C93182 SEX: FEMALE DATE OF DEATH: 04/24/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/24/00 10:05 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 506.4 GRAMS PROSECTOR: JOHN S. HALFORD RECORDER: JERALD W. JOHNSON PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); AXILLARY REGION-LEFT; WEEK 102; 15 X 15MM; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -RED FOCUS(I)/AREA(S); DIAPHRAGMATIC SURFACE OF RIGHT MEDIAN LOBE: SINGLE DARK RED FOCUS, 5 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION -CYST(S); LEFT LATERAL LOBE ALONG MARGIN: SINGLE CLEAR FLUID FILLED CYST, 3 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION PITUITARY (PI) : -MASS(ES); 7 X 5 X 4 MM, DARK RED AND SOFT HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC CELL,-MINIMAL -CYST, BILIARY,-PRESENT CLEAR / KIDNEY (KD) : -MINERALIZATION, PELVIC,-MODERATE -HYPERPLASIA, TRANSITIONAL CELL,-SLIGHT PANCREAS (PA) : -INFILTRATION, FAT, INTERSTITIAL,MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : >UNREMARKABLE 3880 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2119 ANIMAL NUMBER: C93182 SEX: FEMALE DATE OF DEATH: 04/24/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/24/00 10:05 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 506.4 GRAMS PROSECTOR: JOHN S. HALFORD RECORDER: JERALD W. JOHNSON PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA, ALONG GREATER CURVATURE: SINGLE BLACK AREA, 2 X 1 MM MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT AXILLARY REGION: SINGLE MASS, 19 X 19 X 10 MM, RED, DARK RED, GRAY, TAN AND SOFT; SAME ON CUT SURFACE (CAP 22); REGIONAL LN (AXILLARY) NORMAL (CAP 24) -MASS #2; LEFT AXILLARY REGION: SINGLE MASS, 33 X 21 X 15 MM, RED, GRAY, TAN, LOBULATED AND SOFT; SAME ON CUT SURFACE (CAP 23); REGIONAL LN (AXILLARY) NORMAL (CAP 24) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN MAMMARY, FEMALE (MF) : -M-CARCINOMA,-NEOPLASM PRESENT -B-FIBROADENOMA,-NEOPLASM PRESENT -HYPERPLASIA, GLANDULAR,-MODERATE >NOTE:>MASS #2 IS THE FIBROADENOMA CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, AXILLARY (AX) : -HYPERPLASIA, LYMPHOPLASMACYTIC,-SLIGHT 3881 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2120 ANIMAL NUMBER: C93182 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 04/24/00 STUDY DAY OF DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 506.4 GRAMS DATE AND TIME OF NECROPSY: 04/24/00 10:05 PROSECTOR: JOHN S. HALFORD RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: OVARY (OV), LUNG (LU), PARATHYROID (PT), URINARY BLADDER (UB) 3882 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2121 ANIMAL NUMBER: C93183 SEX: FEMALE DATE OF DEATH: 04/24/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/24/00 8:37 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 772.2 GRAMS PROSECTOR: LAURIE J. SCHULLER RECORDER: JERALD W. JOHNSON PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); INGUINAL REGION-LEFT; WEEK 91; 125 X 100MM; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY ADRENAL, CORTEX (AC) : -LARGE; LEFT: 7 X 5 X 4 MM (RIGHT IS 4 MM IN DIAMETER) SKIN (SK) : >NOTE:>COLLECTED FROM RIGHT INGUINAL REGION DUE TO MF MASS HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-MODERATE -HYPERPLASIA, BILE DUCT,-MINIMAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-MODERATE ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC / HEMORRHAGIC,MODERATELY SEVERE -PIGMENT,-MINIMAL SKIN (SK) : >NOT REQUIRED TO BE EXAMINED FOR ANIMAL MAMMARY, FEMALE (MF) : -B-FIBROADENOMA,-NEOPLASM PRESENT 3883 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2122 ANIMAL NUMBER: C93183 SEX: FEMALE DATE OF DEATH: 04/24/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/24/00 8:37 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 772.2 GRAMS PROSECTOR: LAURIE J. SCHULLER RECORDER: JERALD W. JOHNSON PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS(ES); LEFT ABDOMINAL REGION EXTENDING TO LEFT INGUINAL REGION: SINGLE MASS, 150 X 100 X 50 MM (327.7 GRAMS), RED, DARK RED, TAN, FIRM, LOBULATED AND VASCULAR; SAME ON CUT SURFACE; PORTIONS COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED; OVERLYING SKIN HAS A SINGLE DARK RED, DRY CRUSTED AREA, 30 X 15 MM; COLLECTED INTACT WITH MASS -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION >NOTE:>COLLECTED FROM RIGHT INGUINAL REGION DUE TO MF MASS UTERUS (UT) : -CYST(S); BILATERAL, WITHIN WALLS: MULTIPLE CLEAR FLUID FILLED CYSTS, UP TO 5 MM IN DIAMETER GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN UTERUS (UT) : -DILATATION,-MODERATE CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 3884 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2123 ANIMAL NUMBER: C93183 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 04/24/00 STUDY DAY OF DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 772.2 GRAMS DATE AND TIME OF NECROPSY: 04/24/00 8:37 PROSECTOR: LAURIE J. SCHULLER RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), THYROID (TY), PARATHYROID (PT), URINARY BLADDER (UB) 3885 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2124 ANIMAL NUMBER: C93184 SEX: FEMALE DATE OF DEATH: 04/24/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/24/00 10:28 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 526.5 GRAMS PROSECTOR: JOHN S. HALFORD RECORDER: JERALD W. JOHNSON PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >WART LIKE LESION(S); MOUTH; WEEK 102; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE RED AREAS, UP TO 4 X 3 MM; COLLECTED ON ROUTINE SECTIONS OVARY (OV) : -CYST(S); RIGHT: SINGLE CLEAR FLUID FILLED CYST, 12 MM IN DIAMETER PITUITARY (PI) : -MASS(ES); 8 X 6 X 6 MM, RED, DARK RED, GRAY, TAN AND SOFT HISTOPATHOLOGY BRAIN (BR) : >UNREMARKABLE LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, PELVIC,-MINIMAL OVARY (OV) : -CYST,-PRESENT LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL 3886 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2125 ANIMAL NUMBER: C93184 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 04/24/00 STUDY DAY OF DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 526.5 GRAMS DATE AND TIME OF NECROPSY: 04/24/00 10:28 PROSECTOR: JOHN S. HALFORD RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY STOMACH, GL (ST) : -EROSION/ULCERATION; MUCOSA: MULTIPLE TAN, SLIGHTLY ERODED AREAS, UP TO 2 X 1 MM SKIN (SK) : -RAISED FOCUS(I)/AREA(S); ATTACHED TO LOWER RIGHT LIP: SINGLE TAN AREA, 5 X 4 X 3 MM (CAP 22) UTERUS (UT) : -CYST(S); BILATERAL, WITHIN WALLS: MULTIPLE CLEAR FLUID FILLED CYSTS, UP TO 8 MM IN DIAMETER GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN SKIN (SK) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT -B-PAPILLOMA, SQUAMOUS CELL,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -HYPERPLASIA, GLANDULAR,-MINIMAL UTERUS (UT) : -DILATATION,-MODERATELY SEVERE CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 3887 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2126 ANIMAL NUMBER: C93184 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 04/24/00 STUDY DAY OF DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 526.5 GRAMS DATE AND TIME OF NECROPSY: 04/24/00 10:28 PROSECTOR: JOHN S. HALFORD RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), PARATHYROID (PT), URINARY BLADDER (UB) 3888 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2127 ANIMAL NUMBER: C93185 SEX: FEMALE DATE OF DEATH: 04/24/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/24/00 9:51 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 521.2 GRAMS PROSECTOR: JODI THIEREN RECORDER: TONE EVERTS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED >EYES; OPAQUE; EYE-LEFT; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY EYE (EY) : -OPAQUE CORNEA; LEFT OVARY (OV) : -CYST(S); LEFT: SINGLE CLEAR FLUID FILLED CYST, 6 X 3 X 3 MM PARATHYROID (PT) : -LARGE; LEFT: 7 X 5 X 4 MM (RIGHT IS 1 MM IN DIAMETER) HISTOPATHOLOGY EYE (EY) : -DYSTROPHY, CORNEAL IN EARLY STAGES, CONSISTS PRIMARILY OF FOCAL MINERALIZATION,-SLIGHT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-SLIGHT -ANGIECTASIS,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, PELVIC,-SLIGHT OVARY (OV) : -HYPERPLASIA, GRANULOSA CELL,-MODERATE LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL THYROID (TY) : -M-CARCINOMA, C-CELL,-NEOPLASM PRESENT PARATHYROID (PT) : >UNREMARKABLE MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT 3889 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2128 ANIMAL NUMBER: C93185 SEX: FEMALE DATE OF DEATH: 04/24/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/24/00 9:51 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 521.2 GRAMS PROSECTOR: JODI THIEREN RECORDER: TONE EVERTS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY UTERUS (UT) : -THICKENED WALL; BILATERAL, DIFFUSE GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -MALOCCLUSION; BILATERAL UPPER INCISORS -DISCHARGE-OCULAR; LEFT: DARK RED, MATERIAL DRY URINARY BLADDER (UB) : -HEMORRHAGE,-SLIGHT -INFILTRATE, LYMPHOHISTIOCYTIC,MODERATE -HYPERPLASIA, TRANSITIONAL CELL,MINIMAL UTERUS (UT) : -HYPERPLASIA, CYSTIC, ENDOMETRIAL,MINIMAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 3890 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2129 ANIMAL NUMBER: C93185 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 04/24/00 STUDY DAY OF DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 521.2 GRAMS DATE AND TIME OF NECROPSY: 04/24/00 9:51 PROSECTOR: JODI THIEREN RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA) 3891 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2130 ANIMAL NUMBER: C93186 SEX: FEMALE DATE OF DEATH: 09/21/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 09/21/99 14:40 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 520 STUDY WEEK OF DEATH: 75 TERMINAL BODY WEIGHT: 458.9 GRAMS PROSECTOR: JARED FISHEL RECORDER: DENNIS HOFFMAN PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); VENTRAL-CERVICAL; WEEK 45; 100 X 110MM; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY HISTOPATHOLOGY LIVER (LI) : -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, PERIPORTAL,-MODERATE -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL -PIGMENT, TUBULAR,-MINIMAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MINIMAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT -PIGMENT,-MINIMAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY) MINIMAL LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT 3892 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2131 ANIMAL NUMBER: C93186 SEX: FEMALE DATE OF DEATH: 09/21/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 09/21/99 14:40 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 520 STUDY WEEK OF DEATH: 75 TERMINAL BODY WEIGHT: 458.9 GRAMS PROSECTOR: JARED FISHEL RECORDER: DENNIS HOFFMAN PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -CYST(S); RIGHT ASPECT: TWO DARK RED FLUID FILLED CYSTS, ONE IS 2 MM IN DIAMETER, THE OTHER IS 1 MM IN DIAMETER MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT AXILLARY REGION, EXTENDS TO LEFT AXILLARY AND VENTRAL CERVICAL REGIONS: SINGLE MASS, 115 X 90 X 35 MM (187.9 GRAMS), RED, TAN, LOBULATED AND VASCULAR; SAME ON CUT SURFACE (PORTIONS COLLECTED); REGIONAL LN (AXILLARY) NORMAL (CAP 22) -CYST(S); ALL REGIONS, DIFFUSE: MULTIPLE WHITE CREAMY FLUID FILLED CYSTS, UP TO 10 X 6 X 5 MM (CAP 21) UTERUS (UT) : -THICKENED WALL; BILATERAL, DIFFUSE GENERAL COMMENT (GC) : -BLOOD FILM TAKEN MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT -B-FIBROADENOMA,-NEOPLASM PRESENT UTERUS (UT) : >UNREMARKABLE CAUSE OF DEATH (CD) : -NEOPLASM, MAMMARY GLAND,-PRESENT LN, AXILLARY (AX) : -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL 3893 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2132 ANIMAL NUMBER: C93186 SEX: FEMALE DATE OF DEATH: 09/21/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 09/21/99 14:40 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 520 STUDY WEEK OF DEATH: 75 TERMINAL BODY WEIGHT: 458.9 GRAMS PROSECTOR: JARED FISHEL RECORDER: DENNIS HOFFMAN PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 3894 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2133 ANIMAL NUMBER: C93187 SEX: FEMALE DATE OF DEATH: 04/13/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/13/00 14:27 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 725 STUDY WEEK OF DEATH: 104 TERMINAL BODY WEIGHT: 385.9 GRAMS PROSECTOR: JARED FISHEL RECORDER: JARED FISHEL PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); INGUINAL REGION-LEFT; WEEK 81; 65 X 60MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); AXILLARY REGION-RIGHT; WEEK 102; 10 X 10MM; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; ATAXIC; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; ROUGH HAIR COAT; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; PALE; ENTIRE BODY; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; HEAD TILT; LEFT; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) HISTOPATHOLOGY BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-SLIGHT -HYPERPLASIA, BILE DUCT,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, PELVIC,-SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SLIGHT ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-SLIGHT -HYPERTROPHY, CORTICAL CELL,-SLIGHT -DEGENERATION, CYSTIC / HEMORRHAGIC,SLIGHT OVARY (OV) : -CYST,-PRESENT 3895 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2134 ANIMAL NUMBER: C93187 SEX: FEMALE DATE OF DEATH: 04/13/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/13/00 14:27 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 725 STUDY WEEK OF DEATH: 104 TERMINAL BODY WEIGHT: 385.9 GRAMS PROSECTOR: JARED FISHEL RECORDER: JARED FISHEL PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY OVARY (OV) : -CYST(S); RIGHT: SINGLE CLEAR FLUID FILLED CYST, 8 MM IN DIAMETER SKIN (SK) : >NOTE:>SKIN AND MAMMARY GLAND SECTION TAKEN FROM RIGHT INGUINAL REGION DUE TO MASS MAMMARY, FEMALE (MF) : -MASS(ES); LEFT INGUINAL REGION, EXTENDING TO MID ABDOMINAL REGION: SINGLE MASS, 70 X 40 X 30 MM, DARK RED, TAN, VASCULAR AND FIRM; SOME SECTIONS EXUDE A TAN CREAMY SEMIFLUID; PORTION COLLECTED; REGIONAL LN (INGUINAL) NORMAL (CAP 22) THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MINIMAL HEART (HT) : -CARDIOMYOPATHY,-MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,MODERATE MAMMARY, FEMALE (MF) : -B-FIBROADENOMA,-NEOPLASM PRESENT -B-ADENOMA,-NEOPLASM PRESENT >NOTE:>MASS #1 IS THE FIBROADENOMA 3896 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2135 ANIMAL NUMBER: C93187 SEX: FEMALE DATE OF DEATH: 04/13/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/13/00 14:27 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 725 STUDY WEEK OF DEATH: 104 TERMINAL BODY WEIGHT: 385.9 GRAMS PROSECTOR: JARED FISHEL RECORDER: JARED FISHEL PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS #2; RIGHT AXILLARY REGION: SINGLE MASS, 15 MM IN DIAMETER, GREEN, BROWN AND SOFT; ON SECTION EXUDES A BROWN SEMIFLUID; COLLECTED INTACT; REGIONAL LN (AXILLARY) NORMAL (CAP 23) UTERUS (UT) : -CYST(S); BILATERAL, WITHIN WALLS: MULTIPLE CLEAR FLUID FILLED CYSTS, UP TO 7 MM IN DIAMETER GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL: LOWER INCISORS -DISCHARGE-OCULAR; BILATERAL: DRY RED MATERIAL UTERUS (UT) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE -HYPERPLASIA, CYSTIC, ENDOMETRIAL,MODERATE VAGINA (VA) : -HYPERPLASIA, MUCINOUS,-SLIGHT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT LN, AXILLARY (AX) : -HYPERPLASIA, LYMPHOPLASMACYTIC,MODERATE LN, INGUINAL (IN) : -HYPERPLASIA, LYMPHOPLASMACYTIC,-SLIGHT 3897 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2136 ANIMAL NUMBER: C93187 SEX: FEMALE DATE OF DEATH: 04/13/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/13/00 14:27 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 725 STUDY WEEK OF DEATH: 104 TERMINAL BODY WEIGHT: 385.9 GRAMS PROSECTOR: JARED FISHEL RECORDER: JARED FISHEL PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), HEMATO NEOPLASIA (HN) 3898 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2137 ANIMAL NUMBER: C93188 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 04/22/00 STUDY DAY OF DEATH: 734 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 04/22/00 8:59 PROSECTOR: JODI THIEREN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); AXILLARY REGION-LEFT; WEEK 70; 100 X 100MM; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; AXILLARY REGION-LEFT; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -LARGE; ALL LOBES: MARGINS APPEAR ROUNDED -DIFFUSELY LIGHT; ALL LOBES: TAN HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -NECROSIS, COAGULATIVE,-SLIGHT -HYPERTROPHY, HEPATOCELLULAR, PERIPORTAL,-MODERATE KIDNEY (KD) : -MINERALIZATION, PELVIC,-SLIGHT SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SEVERE ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-MINIMAL MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,-MODERATE MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,-MODERATE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATE LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT 3899 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2138 ANIMAL NUMBER: C93188 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 04/22/00 STUDY DAY OF DEATH: 734 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 04/22/00 8:59 PROSECTOR: JODI THIEREN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: MULTIPLE BROWN AREAS, UP TO 2 MM IN DIAMETER SKIN (SK) : >NOTE:>SKIN TAKEN FROM RIGHT INGUINAL REGION DUE TO MASS ON LEFT MAMMARY, FEMALE (MF) : -MASS(ES); LEFT AXILLARY REGION EXTENDING TO LEFT INGUINAL REGION: SINGLE MASS, 130 X 100 X 65 MM (292.7 GRAMS), RED, DARK RED, TAN, GRAY, VASCULAR, LOBULATED AND FIRM; SAME ON CUT SURFACE; PORTION COLLECTED; REGIONAL LN (SEE AXILLARY); OVERLYING SKIN HAS A SINGLE DARK RED AND DRY CRUSTED AREA, 25 X 16 MM; COLLECTED INTACT WITH MASS >NOTE:>MAMMARY SECTION TAKEN FROM RIGHT INGUINAL REGION DUE TO MASS ON LEFT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : >UNREMARKABLE CECUM (CE) : >AUTOLYTIC MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT -B-FIBROADENOMA,-NEOPLASM PRESENT -HYPERPLASIA, GLANDULAR,-MODERATE CAUSE OF DEATH (CD) : -NEOPLASM, MAMMARY GLAND,-PRESENT LN, AXILLARY (AX) : -HYPERPLASIA, LYMPHOPLASMACYTIC,-SEVERE 3900 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2139 ANIMAL NUMBER: C93188 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 04/22/00 STUDY DAY OF DEATH: 734 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 04/22/00 8:59 PROSECTOR: JODI THIEREN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY LN, AXILLARY (AX) : -LARGE; LEFT: UP TO 10 X 7 X 3 MM (RIGHT IS UP TO 6 X 3 X 2 MM) (CAP 21) -MOTTLED; RIGHT: DARK RED AND TAN -DIFFUSELY RED; LEFT GENERAL COMMENT (GC) : -ANIMAL OBESE 3901 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2140 ANIMAL NUMBER: C93188 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 04/22/00 STUDY DAY OF DEATH: 734 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 04/22/00 8:59 PROSECTOR: JODI THIEREN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUm /s KULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 3902 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2141 ANIMAL NUMBER: C93189 SEX: FEMALE DATE OF DEATH: 12/15/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 12/15/99 13:47 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 605 STUDY WEEK OF DEATH: 87 TERMINAL BODY WEIGHT: 220.8 GRAMS PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; HUNCHED; VERIFIED AT NECROPSY: NOT APPLICABLE >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; HEAD TILT; LEFT; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 11 X 11 X 10 MM, RED, GREY AND SOFT HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MINIMAL PANCREAS (PA) : -HYPERTROPHY / BASOPHILIA, ACINAR CELL, MINIMAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MINIMAL -HYPERTROPHY, CORTICAL CELL,-MINIMAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATE PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT 3903 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2142 ANIMAL NUMBER: C93189 SEX: FEMALE DATE OF DEATH: 12/15/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 12/15/99 13:47 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 605 STUDY WEEK OF DEATH: 87 TERMINAL BODY WEIGHT: 220.8 GRAMS PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: SINGLE DARK BROWN FOCUS, 3 X 2 MM VAGINA (VA) : -SEMIFLUID MATERIAL; WITHIN LUMEN: LIGHT GREEN, MUCOID MATERIAL GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; UPPER RIGHT INCISOR -ANIMAL THIN STOMACH, GL (ST) : -EROSION,-MINIMAL MAMMARY, FEMALE (MF) : -HYPERPLASIA, GLANDULAR,-SLIGHT CERVIX (CV) : -HYPERPLASIA, MUCINOUS,-MINIMAL VAGINA (VA) : -HYPERPLASIA, MUCINOUS,-SLIGHT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 3904 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2143 ANIMAL NUMBER: C93189 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/15/99 STUDY DAY OF DEATH: 605 STUDY WEEK OF DEATH: 87 TERMINAL BODY WEIGHT: 220.8 GRAMS DATE AND TIME OF NECROPSY: 12/15/99 13:47 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUm /s KULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), HEMATO NEOPLASIA (HN) 3905 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2144 ANIMAL NUMBER: C93190 SEX: FEMALE DATE OF DEATH: 04/24/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/24/00 10:56 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 280.7 GRAMS PROSECTOR: JODI THIEREN RECORDER: TONE EVERTS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; ROUGH HAIR COAT; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES) ; 12 X 8 X 8 MM, RED, DARK RED, TAN , GREY, AND SOFT STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: SINGLE BLACK LINEAR AREA, 2 X 1 MM HISTOPATHOLOGY BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-SLIGHT -HYPERPLASIA, TRANSITIONAL CELL,MINIMAL -INFLAMMATION, SUPPURATIVE, PELVIC,MINIMAL PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-SLIGHT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : -EROSION,-MINIMAL MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT -HYPERPLASIA, GLANDULAR,-SLIGHT 3906 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2145 ANIMAL NUMBER: C93190 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 04/24/00 STUDY DAY OF DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 280.7 GRAMS DATE AND TIME OF NECROPSY: 04/24/00 10:56 PROSECTOR: JODI THIEREN RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -ANIMAL THIN URINARY BLADDER (UB) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), THYROID (TY), PARATHYROID (PT) 3907 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2146 ANIMAL NUMBER: C93191 SEX: FEMALE DATE OF DEATH: 01/12/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 01/12/00 15:19 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 633 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: 539.2 GRAMS PROSECTOR: GREGORY CODDINGTON RECORDER: JODI THIEREN PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >EYES; RED DISCHARGE; EYES; VERIFIED AT NECROPSY: NOT OBSERVED >RESPIRATION; AUDIBLE; VERIFIED AT NECROPSY: NOT APPLICABLE >RESPIRATION; LABORED; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; RECUMBENT; LATERAL; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -HEPATIC ANOMALY (LOBULE); BETWEEN MEDIAN LOBES, NEAR HILUS: SINGLE ANOMALY, 10 X 10 X 6 MM; COLLECTED HISTOPATHOLOGY BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -VACUOLATION, HEPATOCELLULAR, MIDZONAL / CENTRILOBULAR,-SLIGHT -ANOMALY, SECONDARY TO DIAPHRAGMATIC HERNIA,-PRESENT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, PELVIC,-MINIMAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE PANCREAS (PA) : -INFILTRATION, FAT, INTERSTITIAL,SLIGHT ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT -PIGMENT,-SLIGHT HEART (HT) : -CARDIOMYOPATHY,-MINIMAL 3908 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2147 ANIMAL NUMBER: C93191 SEX: FEMALE DATE OF DEATH: 01/12/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 01/12/00 15:19 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 633 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: 539.2 GRAMS PROSECTOR: GREGORY CODDINGTON RECORDER: JODI THIEREN PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 7 X 7 X 6 MM, DARK RED AND SOFT MAMMARY, FEMALE (MF) : -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; RIGHT: CLEAR AND MOIST THYROID (TY) : -HYPERPLASIA, C-CELL,-SLIGHT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT -HYPERPLASIA, GLANDULAR,-SLIGHT UTERUS (UT) : -DILATATION,-MINIMAL CERVIX (CV) : -HYPERPLASIA, MUCINOUS,-MODERATE VAGINA (VA) : -HYPERPLASIA, MUCINOUS,-SEVERE CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 3909 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2148 ANIMAL NUMBER: C93191 SEX: FEMALE DATE OF DEATH: 01/12/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 01/12/00 15:19 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 633 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: 539.2 GRAMS PROSECTOR: GREGORY CODDINGTON RECORDER: JODI THIEREN PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), MUSCLE SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE) COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), HEMATO NEOPLASIA (HN) (SG), 3910 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2149 ANIMAL NUMBER: C93192 SEX: FEMALE DATE OF DEATH: 04/24/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/24/00 10:05 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 519.3 GRAMS PROSECTOR: JAMES T. CALLEN RECORDER: JERALD W. JOHNSON PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); VENTRAL-ABDOMINAL-RIGHT; WEEK 91; 70 X 75MM; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE DARK RED AREAS, UP TO 3 X 2 MM; COLLECTED ON ROUTINE SECTIONS THYMUS (TH) : -MISSING/NOT IDENTIFIED; NOT POSITIVELY IDENTIFIED; POSSIBLE REMNANTS COLLECTED PITUITARY (PI) : -LARGE; 6 X 6 X 3 MM HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-SLIGHT -FIBROSIS, PERIBILIARY,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, PERIPORTAL,-SLIGHT -CYST, BILIARY,-PRESENT -B-ADENOMA, HEPATOCELLULAR,-NEOPLASM PRESENT KIDNEY (KD) : -MINERALIZATION, PELVIC,-SLIGHT THYMUS (TH) : -CYST,-PRESENT -HYPERPLASIA, EPITHELIAL,-MODERATE THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT 3911 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2150 ANIMAL NUMBER: C93192 SEX: FEMALE DATE OF DEATH: 04/24/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/24/00 10:05 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 519.3 GRAMS PROSECTOR: JAMES T. CALLEN RECORDER: JERALD W. JOHNSON PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -RED FOCUS(I)/AREA(S); DIFFUSE: MULTIPLE DARK RED AREAS, UP TO 2 X 1 MM MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT INGUINAL REGION: SINGLE MASS, 75 X 60 X 30 MM, TAN, LOBULATED, VASCULAR AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (INGUINAL) NOT IDENTIFIED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT -B-FIBROADENOMA,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 3912 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2151 ANIMAL NUMBER: C93192 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 04/24/00 STUDY DAY OF DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 519.3 GRAMS DATE AND TIME OF NECROPSY: 04/24/00 10:05 PROSECTOR: JAMES T. CALLEN RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), LUNG (LU), PARATHYROID (PT), URINARY BLADDER (UB) 3913 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2152 ANIMAL NUMBER: C93193 SEX: FEMALE DATE OF DEATH: 10/01/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 10/01/99 14:28 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 530 STUDY WEEK OF DEATH: 76 TERMINAL BODY WEIGHT: 420.9 GRAMS PROSECTOR: LYNN HORNER RECORDER: MICHELE MARGGI PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; RED HAIR COAT; HEADENTIRE; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; YELLOW HAIR COAT; VERIFIED AT NECROPSY: NOT OBSERVED >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >EXCRETION; NONFORMED FECES; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) ADRENAL, CORTEX (AC) : -DARK FOCUS(I)/AREA(S); LEFT: MULTIPLE DARK BROWN AREAS, UP TO 2 MM IN DIAMETER HISTOPATHOLOGY BRAIN (BR) : >UNREMARKABLE LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -VACUOLATION, HEPATOCELLULAR,-SEVERE KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, PELVIC,-SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-MODERATE ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT -PIGMENT,-MINIMAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),SLIGHT HEART (HT) : -CARDIOMYOPATHY,-MINIMAL 3914 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2153 ANIMAL NUMBER: C93193 SEX: FEMALE DATE OF DEATH: 10/01/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 10/01/99 14:28 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 530 STUDY WEEK OF DEATH: 76 TERMINAL BODY WEIGHT: 420.9 GRAMS PROSECTOR: LYNN HORNER RECORDER: MICHELE MARGGI PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); SINGLE MASS, 10 X 9 X 6 MM, RED, TAN AND SOFT MAMMARY, FEMALE (MF) : -CYST(S); ALL REGIONS, DIFFUSE: MULTIPLE TAN CREAMY SEMIFLUID FILLED CYSTS, UP TO 6 MM IN DIAMETER (CAP 21) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL OBESE -STAINS - SKIN/HAIRCOAT; RED AND DRY ENTIRE HEAD: THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 3915 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2154 ANIMAL NUMBER: C93193 SEX: FEMALE DATE OF DEATH: 10/01/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 10/01/99 14:28 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 530 STUDY WEEK OF DEATH: 76 TERMINAL BODY WEIGHT: 420.9 GRAMS PROSECTOR: LYNN HORNER RECORDER: MICHELE MARGGI PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 3916 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2155 ANIMAL NUMBER: C93194 SEX: FEMALE DATE OF DEATH: 01/17/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 01/17/00 13:51 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 638 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: 311.9 GRAMS PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; ROUGH HAIR COAT; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; SORE/SCAB; VENTRALTHORACIC; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK 82; 10 X 10MM; VERIFIED AT NECROPSY: NOT OBSERVED >SKIN & PELAGE; ALOPECIA; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; VENTRALABDOMINAL-RIGHT; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY HISTOPATHOLOGY EYE (EY) : -INFLAMMATION, ACUTE,-SLIGHT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL KIDNEY (KD) : -MINERALIZATION, TUBULAR,-MINIMAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SLIGHT -HYPERPLASIA, LYMPHORETICULAR,-MODERATE PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-MINIMAL -ATROPHY, ACINAR,-SLIGHT ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATELY SEVERE -HYPERPLASIA, CORTICAL,-MINIMAL OVARY (OV) : -CYST,-PRESENT MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,-SEVERE MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,-SEVERE 3917 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2156 ANIMAL NUMBER: C93194 SEX: FEMALE DATE OF DEATH: 01/17/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 01/17/00 13:51 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 638 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: 311.9 GRAMS PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 6 MM IN DIAMETER, DARK RED AND SOFT SKIN (SK) : -CRUSTED AREA(S); ALL REGIONS DIFFUSE: MULTIPLE TAN DRY CRUSTED AREAS, UP TO 25 X 7 MM; COLLECTED ON ROUTINE SECTION -ALOPECIA-DIFFUSE; ALL REGIONS, DIFFUSE: COLLECTED ON ROUTINE SECTION THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),SLIGHT LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL -INFILTRATE, MACROPHAGE, ALVEOLAR,SLIGHT -INFILTRATE, LYMPHOHISTIOCYTIC,-SLIGHT HEART (HT) : -CARDIOMYOPATHY,-MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-SLIGHT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -ATROPHY,-SLIGHT RECTUM (RE) : -PARASITISM,-PRESENT SKIN (SK) : -ACANTHOSIS/HYPERKERATOSIS,-SLIGHT -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE -ULCERATION,-SLIGHT MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT 3918 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2157 ANIMAL NUMBER: C93194 SEX: FEMALE DATE OF DEATH: 01/17/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 01/17/00 13:51 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 638 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: 311.9 GRAMS PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY LN, AXILLARY (AX) : -LARGE; BILATERAL: LEFT IS 20 X 15 X 5 MM, RIGHT IS 13 X 10 X 4 MM (CAP 21) -DIFFUSELY RED; RIGHT: DARK RED LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL -LARGE; BILATERAL: UP TO 15 X 10 X 4 MM; COLLECTED LN, LUMBAR/ILIAC (LM) : -LARGE; LEFT: 15 X 10 X 4 MM 6 X 5 X 3 MM) (CAP 22) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN (RIGHT IS CAUSE OF DEATH (CD) : -INFLAMMATION / INFECTION,-PRESENT LN, AXILLARY (AX) : -HEMORRHAGE,-PRESENT -HYPERPLASIA, LYMPHOPLASMACYTIC,-SEVERE -INFILTRATE, MACROPHAGE, PIGMENTED,SLIGHT -FIBROPLASIA, PERIPHERAL,-SEVERE LN, MANDIBULAR (MN) : -HYPERPLASIA, LYMPHOPLASMACYTIC,-SEVERE -HEMORRHAGE,-PRESENT -INFILTRATE, MACROPHAGE, PIGMENTED,SLIGHT -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE LN, LUMBAR/ILIAC (LM) : -HYPERPLASIA, LYMPHOPLASMACYTIC,-SEVERE -INFLAMMATION, CHRONIC-ACTIVE,-SEVERE 3919 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2158 ANIMAL NUMBER: C93194 SEX: FEMALE DATE OF DEATH: 01/17/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 01/17/00 13:51 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 638 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: 311.9 GRAMS PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), TAIL (TI), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: BRAIN (BR), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 3920 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2159 ANIMAL NUMBER: C93195 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 08/23/99 STUDY DAY OF DEATH: 491 STUDY WEEK OF DEATH: 71 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 08/23/99 11:25 PROSECTOR: DAVID SCHUETTE RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) THYMUS (TH) : -RED FOCUS(I)/AREA(S); DIFFUSE: MULTIPLE DARK RED AREAS, UP TO 1 MM IN DIAMETER PITUITARY (PI) : -MASS(ES); 10 X 8 X 5 MM, DARK RED, TAN AND SOFT HISTOPATHOLOGY BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL -MINERALIZATION, TUBULAR,-SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC / HEMORRHAGIC,MODERATE -PIGMENT,-MINIMAL THYMUS (TH) : -HEMORRHAGE,-PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -M-CARCINOMA,-NEOPLASM PRESENT -CYST / GALACTOCELE,-PRESENT 3921 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2160 ANIMAL NUMBER: C93195 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 08/23/99 STUDY DAY OF DEATH: 491 STUDY WEEK OF DEATH: 71 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 08/23/99 11:25 PROSECTOR: DAVID SCHUETTE RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS(ES); VENTRAL THORACIC REGION: TWO MASSES, ONE IS 15 X 12 X 6 MM, THE OTHER IS 11 X 7 X 6 MM, BOTH ARE TAN AND SOFT; SAME ON BOTH CUT SURFACES; THE LARGER MASS EXUDES A TAN CASEOUS SEMISOLID MATERIAL; BOTH COLLECTED; REGIONAL LN (AXILLARY) NOT IDENTIFIED MAMMARY, FEMALE (MF) : -B-FIBROADENOMA,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 3922 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2161 ANIMAL NUMBER: C93195 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 08/23/99 STUDY DAY OF DEATH: 491 STUDY WEEK OF DEATH: 71 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 08/23/99 11:25 PROSECTOR: DAVID SCHUETTE RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG), GENERAL COMMENT (GC) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 3923 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2162 ANIMAL NUMBER: C93196 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/24/99 STUDY DAY OF DEATH: 614 STUDY WEEK OF DEATH: 88 TERMINAL BODY WEIGHT: 304.7 GRAMS DATE AND TIME OF NECROPSY: 12/24/99 8:02 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >EYES; RED DISCHARGE; EYES; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -HEMORRHAGE,-MINIMAL LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-SLIGHT -HYPERPLASIA, BILE DUCT,-MINIMAL -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, PELVIC,-MODERATE -INFLAMMATION, SUPPURATIVE, PELVIC,SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATELY SEVERE PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-MODERATE ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT -HYPERTROPHY, CORTICAL CELL,-SLIGHT 3924 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2163 ANIMAL NUMBER: C93196 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/24/99 STUDY DAY OF DEATH: 614 STUDY WEEK OF DEATH: 88 TERMINAL BODY WEIGHT: 304.7 GRAMS DATE AND TIME OF NECROPSY: 12/24/99 8:02 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 12 X 9 X 7 MM, DARK RED, RED, TAN, AND SOFT VAGINA (VA) : -SEMIFLUID MATERIAL; WITHIN LUMEN: CONTAINS A DARK RED SEMIFLUID GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DARK RED AND DRY ADRENAL, CORTEX (AC) : -PIGMENT,-SLIGHT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT CERVIX (CV) : -HYPERPLASIA, STROMAL,-MINIMAL VAGINA (VA) : -LUMEN, EXUDATE,-PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 3925 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2164 ANIMAL NUMBER: C93196 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/24/99 STUDY DAY OF DEATH: 614 STUDY WEEK OF DEATH: 88 TERMINAL BODY WEIGHT: 304.7 GRAMS DATE AND TIME OF NECROPSY: 12/24/99 8:02 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), HEMATO NEOPLASIA (HN) 3926 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2165 ANIMAL NUMBER: C93197 SEX: FEMALE DATE OF DEATH: 04/24/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/24/00 10:25 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 334.0 GRAMS PROSECTOR: TROY ELLENBECKER RECORDER: TONE EVERTS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; SORE/SCAB; PAW-HINDLEFT; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); INGUINAL REGION-LEFT; WEEK 93; 20 X 20MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); INGUINAL REGION-LEFT; WEEK 95; 40 X 40MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); AXILLARY REGION-LEFT; WEEK 99; 10 X 10MM; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) OVARY (OV) : -CYST(S); RIGHT: SINGLE CLEAR FLUID FILLED CYST, 12 X 8 X 6 MM PITUITARY (PI) : -MASS(ES); 9 X 6 X 3 MM, RED, DARK RED, TAN, AND SOFT SKIN (SK) : >NOTE:>ROUTINE SECTION TAKEN FROM RIGHT INGUINAL REGION DUE TO MASS HISTOPATHOLOGY BRAIN (BR) : >UNREMARKABLE LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-SLIGHT -NECROSIS, COAGULATIVE,-SLIGHT -HYPERPLASIA, BILE DUCT,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, PERIPORTAL,-MODERATE KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL OVARY (OV) : -CYST,-PRESENT LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT SKIN (SK) : >NOT REQUIRED TO BE EXAMINED FOR ANIMAL 3927 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2166 ANIMAL NUMBER: C93197 SEX: FEMALE DATE OF DEATH: 04/24/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/24/00 10:25 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 334.0 GRAMS PROSECTOR: TROY ELLENBECKER RECORDER: TONE EVERTS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS(ES); LEFT AXILLARY REGION: SINGLE MASS, 215 X 10 X 4 MM, RED, DARK RED, AND SOFT; SAME ON CUT SURFACE; SOME SECTIONS EXUDE A BROWN SEMISOLID (CAP 23); REGIONAL LN (AXILLARY) NORMAL (CAP 24) -MASS #2; LEFT INGUINAL REGION: MULTIPLE MASSES, UP TO 45 X 35 X 25 MM, RED, DARK RED, TAN, FIRM, AND LOBULATED; SOME SECTIONS EXUDE A TAN CREAMY SEMIFLUID; COLLECTED INTACT; REGIONAL LN (INGUINAL) NOT IDENTIFIED >NOTE:>ROUTINE SECTION TAKEN FROM RIGHT INGUINAL REGION DUE TO MASS FOOT/FOOTPAD (FP) : -ABRASION(S)/ULCERATION(S); LEFT HINDFOOT: SINGLE MOIST AND RED AREA, 6 X 5 MM; COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT -B-FIBROADENOMA,-NEOPLASM PRESENT >NOTE:>MASS #2 IS THE FIBROADENOMA MASS #1 CONSISTS OF GALACTOCELES CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT FOOT/FOOTPAD (FP) : -INFLAMMATION, CHRONIC-ACTIVE,-MODERATE -ULCERATION,-MODERATE -ACANTHOSIS / HYPERKERATOSIS,-MODERATE -FIBROSIS,-SLIGHT 3928 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2167 ANIMAL NUMBER: C93197 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 04/24/00 STUDY DAY OF DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 334.0 GRAMS DATE AND TIME OF NECROPSY: 04/24/00 10:25 PROSECTOR: TROY ELLENBECKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), THYROID (TY), PARATHYROID (PT), URINARY BLADDER (UB), LN, AXILLARY (AX) 3929 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2168 ANIMAL NUMBER: C93198 SEX: FEMALE DATE OF DEATH: 04/24/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/24/00 10:25 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 555.3 GRAMS PROSECTOR: LAURIE J. SCHULLER RECORDER: JERALD W. JOHNSON PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); AXILLARY REGION-RIGHT; WEEK 94; 10 X 10MM; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY PITUITARY (PI) : -CYST(S); LEFT AND RIGHT POSTERIOR ASPECTS: TWO CLEAR FLUID FILLED CYSTS, EACH IS 1 MM IN DIAMETER MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT AXILLARY REGION: SINGLE MASS, 27 X 27 X 10 MM, TAN, WHITE AND FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (AXILLARY) NORMAL (CAP 23) -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -NECROSIS, INDIVIDUAL HEPATOCYTE,MINIMAL -HYPERTROPHY, HEPATOCELLULAR, PERIPORTAL,-SLIGHT KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL PANCREAS (PA) : -INFILTRATION, FAT, INTERSTITIAL,MODERATE PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -B-FIBROADENOMA,-NEOPLASM PRESENT -HYPERPLASIA, GLANDULAR,-MINIMAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 3930 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2169 ANIMAL NUMBER: C93198 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 04/24/00 STUDY DAY OF DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 555.3 GRAMS DATE AND TIME OF NECROPSY: 04/24/00 10:25 PROSECTOR: LAURIE J. SCHULLER RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL: (CAP 22) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -ANIMAL OBESE DARK RED LN, MANDIBULAR (MN) : -HEMORRHAGE,-PRESENT THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG),STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP),JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM(DP), CAVITY, THORACIC (TA),LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), THYROID (TY), PARATHYROID (PT), URINARY BLADDER (UB), LN, AXILLARY (AX) 3931 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2170 ANIMAL NUMBER: C93199 SEX: FEMALE DATE OF DEATH: 07/13/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 07/13/99 8:44 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 450 STUDY WEEK OF DEATH: 65 TERMINAL BODY WEIGHT: 319.8 GRAMS PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; THIN; VERIFIED AT NECROPSY: NOT OBSERVED >APPEARANCE; HEAD TILT; RIGHT; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) HISTOPATHOLOGY BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-SLIGHT -GRANULAR CYTOPLASM, EOSINOPHILIC, CENTRILOBULAR,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, TUBULAR,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : -HYPERTROPHY, CORTICAL CELL,-MINIMAL -PIGMENT,-MINIMAL LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT 3932 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2171 ANIMAL NUMBER: C93199 SEX: FEMALE DATE OF DEATH: 07/13/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 07/13/99 8:44 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 450 STUDY WEEK OF DEATH: 65 TERMINAL BODY WEIGHT: 319.8 GRAMS PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 12 X 10 X 10 MM, DARK RED TAN, AND SOFT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT -B-FIBROADENOMA,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 3933 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2172 ANIMAL NUMBER: C93199 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/13/99 STUDY DAY OF DEATH: 450 STUDY WEEK OF DEATH: 65 TERMINAL BODY WEIGHT: 319.8 GRAMS DATE AND TIME OF NECROPSY: 07/13/99 8:44 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP),JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM(DP), CAVITY, THORACIC (TA),LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG),STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 3934 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2173 ANIMAL NUMBER: C93200 SEX: FEMALE DATE OF DEATH: 02/19/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 02/19/00 8:06 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 671 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: 314.1 GRAMS PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; YELLOW HAIR COAT; VERIFIED AT NECROPSY: OBSERVED >EYES; RED DISCHARGE; EYES; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >EXCRETION; NO FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE DARK RED AREAS, UP TO 3 X 2 MM; COLLECTED ON ROUTINE SECTIONS HISTOPATHOLOGY BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-MODERATELY SEVERE -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, PELVIC,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MINIMAL -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SLIGHT PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-SLIGHT -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -INFILTRATION, FAT, INTERSTITIAL,MINIMAL ADRENAL, CORTEX (AC) : -VACUOLATION, FOCAL,-MODERATE -HYPERTROPHY, CORTICAL CELL,-MODERATE 3935 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2174 ANIMAL NUMBER: C93200 SEX: FEMALE DATE OF DEATH: 02/19/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 02/19/00 8:06 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 671 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: 314.1 GRAMS PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); SINGLE MASS, 9 X 8 X 5 MM, DARK RED, GRAY, TAN AND SOFT MAMMARY, FEMALE (MF) : -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION UTERUS (UT) : -LUMEN FILLED WITH FLUID; BILATERAL: CLEAR FLUID -LARGE, DIFFUSE; BILATERAL: EACH HORN IS 50 X 13 X 10 MM OVARY (OV) : -HYPERPLASIA, GRANULOSA CELL,-SEVERE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATE LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL HEART (HT) : -CARDIOMYOPATHY,-MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-SLIGHT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, NONGL (SU) : -ACANTHOSIS / HYPERKERATOSIS,MODERATELY SEVERE MAMMARY, FEMALE (MF) : -HYPERPLASIA, GLANDULAR,-MODERATE UTERUS (UT) : -DILATATION,-SEVERE 3936 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2175 ANIMAL NUMBER: C93200 SEX: FEMALE DATE OF DEATH: 02/19/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 02/19/00 8:06 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 671 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: 314.1 GRAMS PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DARK RED AND DRY -ANIMAL THIN -STAINS-PERINEUM/PERIANAL; YELLOW AND MOIST CERVIX (CV) : -HYPERPLASIA, MUCINOUS,-SEVERE VAGINA (VA) : -HYPERPLASIA, MUCINOUS,-SEVERE CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 3937 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2176 ANIMAL NUMBER: C93200 SEX: FEMALE DATE OF DEATH: 02/19/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 02/19/00 8:06 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 671 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: 314.1 GRAMS PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), HEMATO NEOPLASIA (HN) 3938 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2177 ANIMAL NUMBER: C93201 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 04/24/00 STUDY DAY OF DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 434.5 GRAMS DATE AND TIME OF NECROPSY: 04/24/00 8:40 PROSECTOR: JODI THIEREN RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE DARK RED AREAS, UP TO 4 MM IN DIAMETER; COLLECTED ON ROUTINE SECTIONS OVARY (OV) : -CYST(S); BILATERAL: MULTIPLE CLEAR FLUID FILLED CYSTS ON EACH, UP TO 1 MM IN DIAMETER PITUITARY (PI) : -DARK FOCUS(I)/AREA(S); MID ASPECT: SINGLE DARK RED AREA, 2 MM IN DIAMETER LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL: DARK RED; COLLECTED HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-SLIGHT -ANGIECTASIS,-SLIGHT KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL OVARY (OV) : -HYPERPLASIA, GRANULOSA CELL,-SEVERE THYROID (TY) : -HYPERPLASIA, C-CELL,-SLIGHT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT -HYPERPLASIA,-MINIMAL -CYST,-PRESENT MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MANDIBULAR (MN) : -HYPERPLASIA, LYMPHOPLASMACYTIC,-SLIGHT -HEMORRHAGE,-PRESENT 3939 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2178 ANIMAL NUMBER: C93201 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 04/24/00 STUDY DAY OF DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 434.5 GRAMS DATE AND TIME OF NECROPSY: 04/24/00 8:40 PROSECTOR: JODI THIEREN RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), LUNG (LU), PARATHYROID (PT), URINARY BLADDER (UB) 3940 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2179 ANIMAL NUMBER: C93202 SEX: FEMALE DATE OF DEATH: 04/24/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/24/00 8:38 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 436.7 GRAMS PROSECTOR: TROY ELLENBECKER RECORDER: TONE EVERTS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LUNG (LU) : -LIGHT FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE TAN AREAS, UP TO 6 X 4 MM GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -NECROSIS, INDIVIDUAL HEPATOCYTE,MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL -VACUOLATION, HEPATOCELLULAR,-MINIMAL LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,MODERATE MAMMARY, FEMALE (MF) : -HYPERPLASIA, GLANDULAR,-MINIMAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 3941 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2180 ANIMAL NUMBER: C93202 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 04/24/00 STUDY DAY OF DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 436.7 GRAMS DATE AND TIME OF NECROPSY: 04/24/00 8:38 PROSECTOR: TROY ELLENBECKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: KIDNEY (KD), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), URINARY BLADDER (UB) 3942 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2181 ANIMAL NUMBER: C93203 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/03/00 STUDY DAY OF DEATH: 624 STUDY WEEK OF DEATH: 90 TERMINAL BODY WEIGHT: 406.2 GRAMS DATE AND TIME OF NECROPSY: 01/03/99 14:31 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); AXILLARY REGION-LEFT; WEEK 76; 15 X 25MM; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; VENTRALABDOMINAL-LEFT; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; VENTRALABDOMINAL-RIGHT; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); INGUINAL REGION-LEFT; WEEK 81; 25 X 30MM; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -DIFFUSELY LIGHT; ALL LOBES: TAN KIDNEY (KD) : -DIFFUSELY LIGHT; BILATERAL: TAN HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL -HEMATOPOIESIS, EXTRAMEDULLARY,-SLIGHT -HYPERTROPHY, HEPATOCELLULAR, PERIPORTAL,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, PELVIC,-MODERATE -HYPERPLASIA, TRANSITIONAL CELL,MINIMAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATELY SEVERE PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-MODERATE ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC / HEMORRHAGIC,MODERATE 3943 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2182 ANIMAL NUMBER: C93203 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/03/00 STUDY DAY OF DEATH: 624 STUDY WEEK OF DEATH: 90 TERMINAL BODY WEIGHT: 406.2 GRAMS DATE AND TIME OF NECROPSY: 01/03/99 14:31 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY OVARY (OV) : -CYST(S); RIGHT: SINGLE CLEAR FLUID FILLED CYST, 4 MM IN DIAMETER PITUITARY (PI) : -MASS(ES); 15 X 15 X 10 MM, DARK RED RED, TAN, AND SOFT ADRENAL, CORTEX (AC) : -PIGMENT,-MINIMAL ADRENAL, MEDULLA (MA) : -HYPERPLASIA, MEDULLARY,-SLIGHT OVARY (OV) : -CYST,-PRESENT MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,-SEVERE MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,MODERATELY SEVERE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MINIMAL LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-SLIGHT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -M-CARCINOMA,- 3 NEOPLASMS PRESENT -CYST / GALACTOCELE,-PRESENT -B-FIBROADENOMA,-NEOPLASM PRESENT -B-ADENOMA,-NEOPLASM PRESENT 3944 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2183 ANIMAL NUMBER: C93203 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/03/00 STUDY DAY OF DEATH: 624 STUDY WEEK OF DEATH: 90 TERMINAL BODY WEIGHT: 406.2 GRAMS DATE AND TIME OF NECROPSY: 01/03/99 14:31 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT AXILLARY REGION: SINGLE MASS, 20 X 10 X 10 MM, DARK RED, BROWN AND SOFT; CUT SECTION CONTAINS A DARK RED FLUID; COLLECTED; REGIONAL LN (SEE AXILLARY) -MASS #2; LEFT AXILLARY REGION: SINGLE MASS, 30 X 30 X 20 MM, TAN, DARK RED, AND FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (SEE AXILLARY) -CYST(S); BILATERAL PECTORAL REGION: MULTIPLE TAN CREAMY SEMIFLUID FILLED CYSTS, UP TO 6 MM IN DIAMETER (CAP 22) -MASS #3; RIGHT INGUINAL REGION: SINGLE MASS, 25 X 20 X 10 MM, BROWN, TAN, AND SOFT; CUT SURFACE CONTAINS A TAN CASEOUS SEMISOLID; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED -MASS #4; LEFT INGUINAL REGION: SINGLE MASS, 60 X 60 X 40 MM, DARK RED, RED, TAN, AND FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED; OVERLYING SKIN HAS TWO DARK RED, TAN, AND MOIST ABRADED AREAS, BOTH ARE 15 MM IN DIAMETER; COLLECTED INTACT; POSSIBLY CONSISTENT WITH SORE/SCAB VENTRAL ABDOMINAL LEFT AND RIGHT NOTED IN CLINICAL OBSERVATIONS MAMMARY, FEMALE (MF) : >NOTE:>THE ADENOMA WAS DESCRIBED AS A CYST MASS #2 IS A FIBROADENOMA MASSES 1,3, AND 4 ARE CARCINOMA MASS 4 ALSO HAS AREAS WITH FEATURES OF FIBROADENOMA 3945 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2184 ANIMAL NUMBER: C93203 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/03/00 STUDY DAY OF DEATH: 624 STUDY WEEK OF DEATH: 90 TERMINAL BODY WEIGHT: 406.2 GRAMS DATE AND TIME OF NECROPSY: 01/03/99 14:31 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY UTERUS (UT) : -THICKENED WALL; BILATERAL, DIFFUSE LN, AXILLARY (AX) : -LARGE; BILATERAL: LEFT IS UP TO 20 X 11 X 7 MM, RIGHT IS UP TO 12 X 10 X 5 MM (CAP 21) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN UTERUS (UT) : -HYPERTROPHY, ENDOMETRIUM,-PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT LN, AXILLARY (AX) : -HYPERPLASIA, LYMPHOPLASMACYTIC,-SEVERE 3946 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2185 ANIMAL NUMBER: C93203 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/03/00 STUDY DAY OF DEATH: 624 STUDY WEEK OF DEATH: 90 TERMINAL BODY WEIGHT: 406.2 GRAMS DATE AND TIME OF NECROPSY: 01/03/99 14:31 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LN, MESENTERIC (MS), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 3947 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2186 ANIMAL NUMBER: C93204 SEX: FEMALE DATE OF DEATH: 01/21/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 01/21/00 7:49 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DEATH: 642 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: NOT ENTERED PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); VENTRAL-ABDOMINAL-RIGHT; WEEK 66; 70 X 80MM; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-SLIGHT -HYPERPLASIA, BILE DUCT,-SLIGHT -DEGENERATION / NECROSIS, CENTRILOBULAR,-MODERATE KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL -MINERALIZATION, TUBULAR,-MINIMAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : -HYPERTROPHY, CORTICAL CELL,-MINIMAL MARROW, FEMUR (FM) : -HYPERPLASIA, ERYTHROID, MARROW,MODERATELY SEVERE MARROW, STERNUM (SE) : -HYPERPLASIA, ERYTHROID, MARROW,MODERATELY SEVERE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY), MODERATELY SEVERE HEART (HT) : -CARDIOMYOPATHY,-MINIMAL 3948 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2187 ANIMAL NUMBER: C93204 SEX: FEMALE DATE OF DEATH: 01/21/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 01/21/00 7:49 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DEATH: 642 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: NOT ENTERED PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -LARGE; 6 X 5 X 3 MM -RED FOCUS(I)/AREA(S); MID ASPECT: SINGLE DARK RED AREA, 3 X 2 MM STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA: SINGLE MOIST TAN ERODED AREA, 3 MM IN DIAMETER MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT INGUINAL REGION: SINGLE MASS, 90 X 89 X 34 MM, RED, TAN, VASCULAR, LOBULATED AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (INGUINAL) NOT IDENTIFIED THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, NONGL (SU) >UNREMARKABLE JEJUNUM (JE) : >AUTOLYTIC ILEUM (IL) : >AUTOLYTIC CECUM (CE) : >AUTOLYTIC MAMMARY, FEMALE (MF) -B-FIBROADENOMA,-NEOPLASM PRESENT -HYPERPLASIA, GLANDULAR,-MODERATE CERVIX (CV) : -HYPERPLASIA, MUCINOUS,-SLIGHT VAGINA (VA) : -HYPERPLASIA, MUCINOUS,-SEVERE CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 3949 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2188 ANIMAL NUMBER: C93204 SEX: FEMALE DATE OF DEATH: 01/21/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 01/21/00 7:49 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DEATH: 642 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: NOT ENTERED PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY CAVITY, ABDOM (PC) : -MASS(ES); NEAR RIGHT OVARY, ATTACHED TO ADIPOSE TISSUE: SINGLE MASS, 25 X 12 X 10 MM, TAN, GREEN, RED AND FIRM; SAME ON CUT SURFACE (CAP 21); REGIONAL LN (LUMBAR) NORMAL (CAP 22) LN, LUMBAR/ILIAC (LM) : -HYPERPLASIA, LYMPHOPLASMACYTIC,MODERATE -HEMORRHAGE,-PRESENT CAVITY, ABDOM (PC) : -INFLAMMATION / NECROSIS, FAT,MODERATELY SEVERE 3950 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2189 ANIMAL NUMBER: C93204 SEX: FEMALE DATE OF DEATH: 01/21/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 01/21/00 7:49 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DEATH: 642 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: NOT ENTERED PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG), GENERAL COMMENT (GC) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), HEMATO NEOPLASIA (HN) 3951 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2190 ANIMAL NUMBER: C93205 SEX: FEMALE DATE OF DEATH: 04/24/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/24/00 8:12 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 428.0 GRAMS PROSECTOR: JOHN S. HALFORD RECORDER: JERALD W. JOHNSON PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -LIGHT FOCUS(I)/AREA(S); VISCERAL SURFACE OF LEFT LATERAL LOBE: SINGLE TAN AREA, 4 X 3 MM; COLLECTED ON ROUTINE SECTION OVARY (OV) : -CYST(S); LEFT: SINGLE CLEAR FLUID FILLED CYST, 11 MM IN DIAMETER PITUITARY (PI) : -MASS(ES); 7 X 5 X 2 MM, RED, DARK RED, GRAY AND SOFT HISTOPATHOLOGY BRAIN (BR) : -I-CARCINOMA,-FROM PITUITARY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MODERATE -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL -DEGENERATION, CYSTIC,-MODERATE KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SLIGHT OVARY (OV) : -CYST,-PRESENT THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : -M-CARCINOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -HYPERPLASIA, GLANDULAR,-MODERATE CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 3952 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2191 ANIMAL NUMBER: C93205 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 04/24/00 STUDY DAY OF DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 428.0 GRAMS DATE AND TIME OF NECROPSY: 04/24/00 8:12 PROSECTOR: JOHN S. HALFORD RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL: (CAP 22) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN DARK RED LN, MANDIBULAR (MN) : -HEMORRHAGE,-PRESENT THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), LUNG (LU), PARATHYROID (PT), URINARY BLADDER (UB) 3953 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2192 ANIMAL NUMBER: C93206 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/15/00 STUDY DAY OF DEATH: 727 STUDY WEEK OF DEATH: 104 TERMINAL BODY WEIGHT: 236.5 GRAMS DATE AND TIME OF NECROPSY: 04/15/00 7:50 PROSECTOR: JARED FISHEL RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >EYES; RED DISCHARGE; EYES; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) HISTOPATHOLOGY BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL LIVER (LI) : -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-SLIGHT -HYPERPLASIA, BILE DUCT,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, PELVIC,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-MODERATE ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATELY SEVERE -HYPERTROPHY, CORTICAL CELL,-MINIMAL -DEGENERATION, CYSTIC / HEMORRHAGIC,MINIMAL -PIGMENT,-MINIMAL 3954 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2193 ANIMAL NUMBER: C93206 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/15/00 STUDY DAY OF DEATH: 727 STUDY WEEK OF DEATH: 104 TERMINAL BODY WEIGHT: 236.5 GRAMS DATE AND TIME OF NECROPSY: 04/15/00 7:50 PROSECTOR: JARED FISHEL RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 12 X 9 X 7 MM, TAN, DARK RED AND SOFT GENERAL COMMENT (GC) -BLOOD FILM TAKEN -DISCHARGE-OCULAR; MATERIAL -ANIMAL THIN : BILATERAL: DRY RED THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),SLIGHT THYROID (TY) : -HYPERPLASIA, C-CELL,-SLIGHT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, NONGL (SU) : -ACANTHOSIS / HYPERKERATOSIS,-SLIGHT -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT VAGINA (VA) : -LUMEN, EXUDATE,-PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 3955 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2194 ANIMAL NUMBER: C93206 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/15/00 STUDY DAY OF DEATH: 727 STUDY WEEK OF DEATH: 104 TERMINAL BODY WEIGHT: 236.5 GRAMS DATE AND TIME OF NECROPSY: 04/15/00 7:50 PROSECTOR: JARED FISHEL RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), HEMATO NEOPLASIA (HN) (SG), 3956 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2195 ANIMAL NUMBER: C93207 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/25/99 STUDY DAY OF DEATH: 281 STUDY WEEK OF DEATH: 41 TERMINAL BODY WEIGHT: 204.3 GRAMS DATE AND TIME OF NECROPSY: 01/25/99 14:02 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); VENTRAL-THORACIC-RIGHT; WEEK 10; 55 X 55MM; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; HUNCHED; VERIFIED AT NECROPSY: NOT APPLICABLE >EYES; RED DISCHARGE; EYES; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -DIFFUSELY DARK; ALL LOBES: DARK BROWN KIDNEY (KD) : -DIFFUSELY DARK; BILATERAL: DARK BROWN -MASS(ES); RIGHT, CORTICAL SURFACE, EXTENDING TO MEDULLA: MULTIPLE MASSES, UP TO 8 X 5 X 3 MM, TAN AND FIRM; SAME ON CUT SURFACES; COLLECTED INTACT; REGIONAL LN (RENAL) NOT IDENTIFIED SPLEEN (SP) : -SMALL; 29 X 5 X 3 MM HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, MACROPHAGE, PIGMENTED,SLIGHT KIDNEY (KD) : -MINERALIZATION, TUBULAR,-MODERATE -N-CARCINOMA,-FROM MAMMARY, FEMALE SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE -DEPLETION, LYMPHOCYTIC,-MODERATELY SEVERE PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-MODERATE LN, MESENTERIC (MS) : -HEMORRHAGE,-PRESENT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATELY SEVERE LUNG (LU) : -N-CARCINOMA,-FROM MAMMARY, FEMALE 3957 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2196 ANIMAL NUMBER: C93207 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/25/99 STUDY DAY OF DEATH: 281 STUDY WEEK OF DEATH: 41 TERMINAL BODY WEIGHT: 204.3 GRAMS DATE AND TIME OF NECROPSY: 01/25/99 14:02 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY LUNG (LU) : -MASS(ES); ALL LOBES: MULTIPLE MASSES, UP TO 15 X 10 X 10 MM, TAN AND FIRM; SAME ON CUT SURFACES; COLLECTED INTACT; REGIONAL LN (TRACHEOBRONCHIAL) NOT IDENTIFIED MAMMARY, FEMALE (MF) : -MASS(ES); VENTRAL THORACIC REGION: SINGLE MASS, 70 X 40 X 30 MM, RED, DARK RED, TAN, BROWN, SOFT, AND LOBULATED; SAME ON CUT SURFACE; ON SECTION, SOME AREAS EXUDED A DARK BROWN, TENACIOUS SEMIFLUID; COLLECTED; REGIONAL LN (SEE AXILLARY) LN, AXILLARY (AX) : -MOTTLED; BILATERAL: DARK BROWN, RED, AND TAN (CAP 20) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DARK RED AND DRY -ANIMAL THIN -GI TRACT-LARGE LUMEN; ENTIRE GI TRACT: DISTENDED WITH GAS STOMACH, NONGL (SU) : -EDEMA,-PRESENT MAMMARY, FEMALE (MF) : -M-CARCINOMA,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, MAMMARY GLAND,-PRESENT LN, AXILLARY (AX) : -HEMORRHAGE, -PRESENT -INFILTRATE, MACROPHAGE, PIGMENTED SLIGHT 3958 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2197 ANIMAL NUMBER: C93207 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/25/99 STUDY DAY OF DEATH: 281 STUDY WEEK OF DEATH: 41 TERMINAL BODY WEIGHT: 204.3 GRAMS DATE AND TIME OF NECROPSY: 01/25/99 14:02 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. RICHARD ALSAKER WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUm /s KULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 3959 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2198 ANIMAL NUMBER: C93208 SEX: FEMALE DATE OF DEATH: 11/16/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 11/16/99 6:55 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DEATH: 576 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: NOT ENTERED PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >RESPIRATION; AUDIBLE; VERIFIED AT NECROPSY: NOT APPLICABLE >RESPIRATION; IRREGULAR; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; RECUMBENT; STERNAL; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) KIDNEY (KD) : -LARGE PELVIS(ES); LEFT ADRENAL, CORTEX (AC) : -SMALL; LEFT: 3 X 2 X 2 MM (RIGHT IS 5 X 4 X 4 MM) OVARY (OV) : -CYST(S); BILATERAL: SINGLE CLEAR FLUID FILLED CYST ON EACH, LEFT IS 6 MM IN DIAMETER, RIGHT IS 3 MM IN DIAMETER HISTOPATHOLOGY BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR POLYKARYOCYTOSIS OCCASIONALLY NOTED,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, PELVIC,-MODERATE -HYPERPLASIA, TRANSITIONAL CELL,-SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT -DEGENERATION, CYSTIC / HEMORRHAGIC,MODERATE OVARY (OV) : -CYST,-PRESENT SPINAL CORD (SC) : -HEMORRHAGE,-SLIGHT 3960 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2199 ANIMAL NUMBER: C93208 SEX: FEMALE DATE OF DEATH: 11/16/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 11/16/99 6:55 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DEATH: 576 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: NOT ENTERED PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); SINGLE MASS, 9 X 7 X 5 MM, RED, TAN, LOBULATED, AND SOFT VAGINA (VA) : -SEMIFLUID MATERIAL; WITHIN LUMEN: LIGHT GREEN CASEOUS SEMIFLUID MATERIAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MINIMAL LUNG (LU) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT -HYPERPLASIA, GLANDULAR,-MODERATE VAGINA (VA) : -LUMEN, EXUDATE,-PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 3961 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2200 ANIMAL NUMBER: C93208 SEX: FEMALE DATE OF DEATH: 11/16/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 11/16/99 6:55 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DEATH: 576 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: NOT ENTERED PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG), GENERAL COMMENT (GC) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), HEMATO NEOPLASIA (HN) 3962 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2201 ANIMAL NUMBER: C93209 SEX: FEMALE DATE OF DEATH: 04/24/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/24/00 10:50 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 604.9 GRAMS PROSECTOR: JAMES T. CALLEN RECORDER: JERALD W. JOHNSON PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY THYROID (TY) : -LARGE; LEFT: 6 X 5 X 5 MM (RIGHT IS 4 X 3 X 2 MM) -DIFFUSELY RED; LEFT: DARK RED HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL -HYPERTROPHY, HEPATOCELLULAR, PERIPORTAL,-SLIGHT KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL PANCREAS (PA) : -INFILTRATION, FAT, INTERSTITIAL,SLIGHT LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL THYROID (TY) : -HYPERPLASIA, C-CELL,-SLIGHT -B-ADENOMA, C-CELL,-NEOPLASM PRESENT JEJUNUM (JE) : -M-CARCINOMA,-NEOPLASM PRESENT 3963 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2202 ANIMAL NUMBER: C93209 SEX: FEMALE DATE OF DEATH: 04/24/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/24/00 10:50 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 604.9 GRAMS PROSECTOR: JAMES T. CALLEN RECORDER: JERALD W. JOHNSON PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY JEJUNUM (JE) : -MASS(ES); WITHIN WALL: SINGLE MASS, 17 X 11 X 10 MM, TAN, LOBULATED, VASCULAR AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (LUMBAR) NORMAL (CAP 22) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN MAMMARY, FEMALE (MF) : -HYPERPLASIA, GLANDULAR,-MODERATE CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, LUMBAR/ILIAC (LM) : -HEMORRHAGE,-PRESENT 3964 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2203 ANIMAL NUMBER: C93209 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 04/24/00 STUDY DAY OF DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 604.9 GRAMS DATE AND TIME OF NECROPSY: 04/24/00 10:50 PROSECTOR: JAMES T. CALLEN RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: URINARY BLADDER (UB) 3965 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2204 ANIMAL NUMBER: C93210 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/22/00 STUDY DAY OF DEATH: 734 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 318.8 GRAMS DATE AND TIME OF NECROPSY: 04/22/00 8:26 PROSECTOR: JODI THIEREN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); INGUINAL REGION-RIGHT; WEEK 88; 30 X 35MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); INGUINAL REGION-LEFT; WEEK 94; 50 X 50MM; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; YELLOW HAIR COAT; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) HISTOPATHOLOGY BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, PELVIC,-MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MINIMAL PANCREAS (PA) : -INFLAMMATION, VASCULAR,-SEVERE ADRENAL, CORTEX (AC) : -HYPERTROPHY, CORTICAL CELL,-MODERATELY SEVERE -DEGENERATION, CYSTIC / HEMORRHAGIC,MODERATE -PIGMENT,-MINIMAL ADRENAL, MEDULLA (MA) : -HYPERPLASIA, MEDULLARY,-MINIMAL 3966 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2205 ANIMAL NUMBER: C93210 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/22/00 STUDY DAY OF DEATH: 734 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 318.8 GRAMS DATE AND TIME OF NECROPSY: 04/22/00 8:26 PROSECTOR: JODI THIEREN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 12 X 9 X 8 MM, RED, DARK RED, TAN AND SOFT SKIN (SK) : >NOTE:>SKIN TAKEN FROM RIGHT INGUINAL REGION DUE TO LARGER MASS ON LEFT MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT INGUINAL REGION: SINGLE MASS, 35 X 26 X 17 MM, TAN, RED, VASCULAR AND FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED -MASS #2; LEFT INGUINAL REGION: SINGLE MASS, 58 X 48 X 33 MM, RED, TAN AND SOFT; ON CUT SECTION EXUDES A TAN CREAMY SEMIFLUID; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY) SLIGHT LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL HEART (HT) : -CARDIOMYOPATHY,-MINIMAL -INFLAMMATION, VASCULAR,-SLIGHT THYROID (TY) : -HYPERPLASIA, C-CELL,-SLIGHT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT -B-FIBROADENOMA,- 2 NEOPLASMS PRESENT -HYPERPLASIA, GLANDULAR,-MINIMAL 3967 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2206 ANIMAL NUMBER: C93210 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/22/00 STUDY DAY OF DEATH: 734 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 318.8 GRAMS DATE AND TIME OF NECROPSY: 04/22/00 8:26 PROSECTOR: JODI THIEREN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY MAMMARY, FEMALE (MF) : >NOTE:>MAMMARY SECTION TAKEN FROM RIGHT INGUINAL REGION DUE TO LARGER MASS ON LEFT VAGINA (VA) : -SEMIFLUID MATERIAL; WITHIN LUMEN: GREEN MUCOID SEMIFLUID MATERIAL GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN -STAINS - SKIN/HAIRCOAT; VENTRAL SURFACE: YELLOW ENTIRE VAGINA (VA) : >UNREMARKABLE CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 3968 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2207 ANIMAL NUMBER: C93210 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/22/00 STUDY DAY OF DEATH: 734 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 318.8 GRAMS DATE AND TIME OF NECROPSY: 04/22/00 8:26 PROSECTOR: JODI THIEREN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUm /s KULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), HEMATO NEOPLASIA (HN) 3969 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2208 ANIMAL NUMBER: C93211 SEX: FEMALE DATE OF DEATH: 04/24/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/24/00 10:25 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 339.0 GRAMS PROSECTOR: JAMES T. CALLEN RECORDER: JERALD W. JOHNSON PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); INGUINAL REGION-LEFT; WEEK 104; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) THYMUS (TH) : -MASS(ES); INVOLVING ENTIRE ORGAN: SINGLE MASS, 11 X 10 X 4 MM, RED AND FIRM; COLLECTED INTACT; REGIONAL LN (SEE MEDIASTINAL) PITUITARY (PI) : -MASS(ES); 10 X 6 X 3 MM, DARK RED, TAN AND SOFT HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -HYPERPLASIA, BILE DUCT,-MODERATE -FIBROSIS, PERIBILIARY,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL THYMUS (TH) : -HYPERPLASIA, LYMPHORETICULAR,-SEVERE LUNG (LU) : -MINERALIZATION, ALVEOLAR LUMINA,MINIMAL THYROID (TY) : -B-ADENOMA, FOLLICULAR CELL,-NEOPLASM PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT 3970 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2209 ANIMAL NUMBER: C93211 SEX: FEMALE DATE OF DEATH: 04/24/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/24/00 10:25 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 339.0 GRAMS PROSECTOR: JAMES T. CALLEN RECORDER: JERALD W. JOHNSON PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS(ES); LEFT INGUINAL REGION: SINGLE MASS, 16 X 12 X 8 MM, TAN AND FIRM; SAME ON CUT SURFACE (CAP 24); REGIONAL LN (INGUINAL) NOT IDENTIFIED UTERUS (UT) : -THICKENED WALL; BILATERAL: DIFFUSE -MASS(ES); ATTACHED TO WALL OF RIGHT HORN, 8 MM FROM BIFURCATION: SINGLE MASS, 20 X 20 X 15 MM, RED, TAN, VASCULAR AND CYSTIC; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (LUMBAR) NORMAL (CAP 23) LN, MEDIASTINAL (ML) : -DIFFUSELY RED; (CAP 22) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN MAMMARY, FEMALE (MF) : -HYPERPLASIA, GLANDULAR,-SLIGHT -B-ADENOMA,-NEOPLASM PRESENT UTERUS (UT) : -HYPERPLASIA, CYSTIC, ENDOMETRIAL,MODERATE -M-LEIOMYOSARCOMA,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MEDIASTINAL (ML) : -HYPERPLASIA, LYMPHOPLASMACYTIC,MODERATE LN, LUMBAR/ILIAC (LM) : -HYPERPLASIA, LYMPHOPLASMACYTIC,MODERATE 3971 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2210 ANIMAL NUMBER: C93211 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 04/24/00 STUDY DAY OF DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 339.0 GRAMS DATE AND TIME OF NECROPSY: 04/24/00 10:25 PROSECTOR: JAMES T. CALLEN RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), PARATHYROID (PT), URINARY BLADDER (UB) 3972 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2211 ANIMAL NUMBER: C93212 SEX: FEMALE DATE OF DEATH: 11/24/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 11/24/99 8:43 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DEATH: 584 STUDY WEEK OF DEATH: 84 TERMINAL BODY WEIGHT: NOT ENTERED PROSECTOR: TODD ACKER RECORDER: TODD ACKER PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; SORE/SCAB; PAWS-HIND; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) ADRENAL, CORTEX (AC) : -LARGE; LEFT: 8 X 6 X 4 MM -SMALL; RIGHT: 3 X 2 X 2 MM OVARY (OV) : -CYST(S); RIGHT: SINGLE RED FLUID FILLED CYST, 8 MM IN DIAMETER HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -HEMATOPOIESIS, EXTRAMEDULLARY,-SLIGHT -HYPERPLASIA, BILE DUCT,-MINIMAL -FIBROSIS, PERIBILIARY,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, PELVIC,-MINIMAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATELY SEVERE PANCREAS (PA) : -ATROPHY, ACINAR,-MINIMAL ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC / HEMORRHAGIC,SLIGHT -HYPERPLASIA, CORTICAL,-MINIMAL OVARY (OV) : >UNREMARKABLE 3973 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2212 ANIMAL NUMBER: C93212 SEX: FEMALE DATE OF DEATH: 11/24/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 11/24/99 8:43 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DEATH: 584 STUDY WEEK OF DEATH: 84 TERMINAL BODY WEIGHT: NOT ENTERED PROSECTOR: TODD ACKER RECORDER: TODD ACKER PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 11 X 7 X 6 MM, DARK RED, TAN AND SOFT STOMACH, GL (ST) : -RAISED AREA(S); MUCOSA, NEAR JUNCTION OF NONGLANDULAR JUNCTION: SINGLE TAN AREA, 2 X 2 X 1 MM FOOT/FOOTPAD (FP) : -CRUSTED AREA(S); LEFT HINDFOOT: SINGLE DARK RED AND SLIGHTLY RAISED AREA, 7 X 5 X 2 MM; COLLECTED; RIGHT NOT IDENTIFIED LN, POPLITEAL (PP) : -LARGE; LEFT: 13 X 8 X 4 MM (CAP 21); RIGHT NOT IDENTIFIED -DIFFUSELY RED; LEFT: DARK RED MARROW, FEMUR (FM) : -HYPERPLASIA, MYELOID, MARROW,-SEVERE MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,-SEVERE THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : >UNREMARKABLE MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT -HYPERPLASIA, GLANDULAR,-SLIGHT VAGINA (VA) : -HYPERPLASIA, MUCINOUS,-MODERATE CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT FOOT/FOOTPAD (FP) : -INFLAMMATION, CHRONIC-ACTIVE,MODERATELY SEVERE -ACANTHOSIS / HYPERKERATOSIS,MODERATELY SEVERE LN, POPLITEAL (PP) : -HYPERPLASIA, PLASMACYTIC,-SEVERE 3974 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2213 ANIMAL NUMBER: C93212 SEX: FEMALE DATE OF DEATH: 11/24/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 11/24/99 8:43 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DEATH: 584 STUDY WEEK OF DEATH: 84 TERMINAL BODY WEIGHT: NOT ENTERED PROSECTOR: TODD ACKER RECORDER: TODD ACKER PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL INFORMATION (XX) : >NOTE:>TISSUE ON SLIDE #22 APPEARS TO BE A NECROTIC FIBROADENOMA. AS THERE WAS NO GROSS DESCRIPTION OF A LARGE SUBCUTANEOUS TUMOR, THE TISSUE CANNOT BE DEFINITIVELY ASSOCIATED WITH THIS ANIMAL. 3975 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2214 ANIMAL NUMBER: C93212 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/24/99 STUDY DAY OF DEATH: 584 STUDY WEEK OF DEATH: 84 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/24/99 8:43 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG), GENERAL COMMENT (GC) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), HEMATO NEOPLASIA (HN) 3976 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2215 ANIMAL NUMBER: C93213 SEX: FEMALE DATE OF DEATH: 11/26/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 11/26/99 13:11 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 586 STUDY WEEK OF DEATH: 84 TERMINAL BODY WEIGHT: 265.3 GRAMS PROSECTOR: TODD ACKER RECORDER: TODD ACKER PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: NOT OBSERVED >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; ATAXIC; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; TREMORS; ENTIRE BODY; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; HUNCHED; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; HEAD TILT; LEFT; VERIFIED AT NECROPSY: NOT APPLICABLE >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; COLD TO TOUCH; ENTIRE BODY; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; PALE; ENTIRE BODY; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) ADRENAL, CORTEX (AC) : -LARGE; LEFT: 7 MM IN DIAMETER (RIGHT IS 5 X 4 X 3 MM) HISTOPATHOLOGY BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -NECROSIS, INDIVIDUAL HEPATOCYTE,MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -INFLAMMATION, CHRONIC-ACTIVE, PELVIC,MINIMAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MINIMAL ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC / HEMORRHAGIC,MODERATELY SEVERE -PIGMENT,-SLIGHT LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT 3977 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2216 ANIMAL NUMBER: C93213 SEX: FEMALE DATE OF DEATH: 11/26/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 11/26/99 13:11 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 586 STUDY WEEK OF DEATH: 84 TERMINAL BODY WEIGHT: 265.3 GRAMS PROSECTOR: TODD ACKER RECORDER: TODD ACKER PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 11 X 10 X 7 MM, DARK RED, RED, TAN, GRAY AND SOFT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL, UPPER INCISORS PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 3978 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2217 ANIMAL NUMBER: C93213 SEX: FEMALE DATE OF DEATH: 11/26/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 11/26/99 13:11 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 586 STUDY WEEK OF DEATH: 84 TERMINAL BODY WEIGHT: 265.3 GRAMS PROSECTOR: TODD ACKER RECORDER: TODD ACKER PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), SALIV GL, MANDIB STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) (SG), 3979 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2218 ANIMAL NUMBER: C93214 SEX: FEMALE DATE OF DEATH: 03/03/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 03/03/00 7:46 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DEATH: 684 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: NOT ENTERED PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL -B-ADENOMA, HEPATOCELLULAR,-NEOPLASM PRESENT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, PELVIC,-SLIGHT SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT -HYPERTROPHY, CORTICAL CELL,-MODERATE THYMUS (TH) : -HEMORRHAGE,-PRESENT -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),SLIGHT 3980 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2219 ANIMAL NUMBER: C93214 SEX: FEMALE DATE OF DEATH: 03/03/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 03/03/00 7:46 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DEATH: 684 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: NOT ENTERED PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -CYST(S); RIGHT ASPECT: SINGLE CYST, 3 MM IN DIAMETER, FILLED WITH A DARK RED FLUID LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL (CAP 21) LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,MODERATE PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT JEJUNUM (JE) : >AUTOLYTIC ILEUM (IL) : >AUTOLYTIC MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT URINARY BLADDER (UB) : -MINERALIZED MATERIAL, LUMEN,-PRESENT UTERUS (UT) : -HYPERPLASIA, CYSTIC, ENDOMETRIAL,SLIGHT CERVIX (CV) : -HYPERPLASIA, MUCINOUS,-SLIGHT VAGINA (VA) : -HYPERPLASIA, MUCINOUS,-MODERATE CAUSE OF DEATH (CD) : -UNDETERMINED MICROSCOPICALLY,-PRESENT LN, MANDIBULAR (MN) : -HYPERPLASIA, LYMPHOPLASMACYTIC,MODERATELY SEVERE -CONGESTION,-PRESENT 3981 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2220 ANIMAL NUMBER: C93214 SEX: FEMALE DATE OF DEATH: 03/03/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 03/03/00 7:46 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DEATH: 684 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: NOT ENTERED PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG), GENERAL COMMENT (GC) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), HEMATO NEOPLASIA (HN) 3982 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2221 ANIMAL NUMBER: C93215 SEX: FEMALE DATE OF DEATH: 12/06/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 12/06/99 14:55 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 596 STUDY WEEK OF DEATH: 86 TERMINAL BODY WEIGHT: 298.3 GRAMS PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; ATAXIC; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 11 X 10 X 8 MM, RED, TAN AND SOFT HISTOPATHOLOGY BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-MODERATELY SEVERE SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATELY SEVERE ADRENAL, CORTEX (AC) : -PIGMENT,-SLIGHT THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),SLIGHT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT VAGINA (VA) : >UNREMARKABLE 3983 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2222 ANIMAL NUMBER: C93215 SEX: FEMALE DATE OF DEATH: 12/06/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 12/06/99 14:55 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 596 STUDY WEEK OF DEATH: 86 TERMINAL BODY WEIGHT: 298.3 GRAMS PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY VAGINA (VA) : -SEMIFLUID MATERIAL; WITHIN LUMEN: LIGHT GREEN, MUCOID SEMIFLUID MATERIAL GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN CAUSE OF DEATH (CD) : -NEOPLASM, PITUITARY,-PRESENT 3984 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2223 ANIMAL NUMBER: C93215 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/06/99 STUDY DAY OF DEATH: 596 STUDY WEEK OF DEATH: 86 TERMINAL BODY WEIGHT: 298.3 GRAMS DATE AND TIME OF NECROPSY: 12/06/99 14:55 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), HEMATO NEOPLASIA (HN) (CE), 3985 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2224 ANIMAL NUMBER: C93216 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 04/24/00 STUDY DAY OF DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 514.4 GRAMS DATE AND TIME OF NECROPSY: 04/24/00 9:36 PROSECTOR: TROY ELLENBECKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); EAR-RIGHT; WEEK 61; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); AXILLARY REGION-RIGHT; WEEK 81; 50 X 60MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); AXILLARY REGION-RIGHT; WEEK 93; 20 X 25MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); VENTRAL-ABDOMINAL-RIGHT; WEEK 96; 40 X 40MM; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; VENTRALABDOMINAL-RIGHT; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK 101; 25 X 25MM; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) ADRENAL, CORTEX (AC) : -LARGE; LEFT: 9 X 6 X 5 MM (RIGHT IS 6 X 4 X 3 MM) PITUITARY (PI) : -MASS(ES); 9 X 6 X 4 MM, RED, DARK RED, TAN, AND SOFT HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL -HYPERPLASIA, BILE DUCT,-SLIGHT -FIBROSIS, PERIBILIARY,-SLIGHT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, PELVIC,-MINIMAL ADRENAL, CORTEX (AC) : >NOT REQUIRED TO BE EXAMINED FOR ANIMAL LUNG (LU) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT -HYPERPLASIA,-MODERATE MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT 3986 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2225 ANIMAL NUMBER: C93216 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 04/24/00 STUDY DAY OF DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 514.4 GRAMS DATE AND TIME OF NECROPSY: 04/24/00 9:36 PROSECTOR: TROY ELLENBECKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT AXILLARY REGION: SINGLE MASS, 70 X 60 X 30 MM, RED, DARK RED, TAN, AND FIRM, SAME ON CUT SURFACE; SOME SECTIONS EXUDE A TAN CASEOUS SEMIFLUID; COLLECTED INTACT; REGIONAL LN (SEE AXILLARY) -MASS #2; RIGHT AXILLARY REGION, CAUDAL TO MASS #1: SINGLE MASS, 35 X 25 X 15 MM, RED, TAN, AND FIRM; SAME ON CUT SURFACE; REGIONAL LN (SEE AXILLARY) -MASS #3; LEFT PERIANAL REGION: SINGLE MASS, 40 X 30 X 15 MM, RED, TAN AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (INGUINAL) NOT IDENTIFIED -MASS #4; RIGHT INGUINAL REGION: SINGLE MASS, 50 X 30 X 20 MM, RED, DARK RED, TAN, AND FIRM; SAME ON CUT SURFACE; SOME SECTIONS EXUDE A TAN CREAMY SEMISOLID; OVERLYING SKIN HAS A SINGLE MOIST, BROWN, ULCERATED AREA, 20 X 15 X 4 MM; COLLECTED INTACT WITH MASS; REGIONAL LN (INGUINAL) NOT IDENTIFIED UTERUS (UT) : -CYST(S); WITHIN LUMEN OF LEFT HORN: MULTIPLE CYSTS, UP TO 7 X 5 X 5 MM MAMMARY, FEMALE (MF) : -INFLAMMATION, CHRONIC-ACTIVE,-SEVERE -B-FIBROADENOMA,- 3 NEOPLASMS PRESENT -B-ADENOMA,-NEOPLASM PRESENT >NOTE:>MASS 4 IS THE ADENOMA UTERUS (UT) : -B-POLYP, ENDOMETRIAL, STROMAL,NEOPLASM PRESENT 3987 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2226 ANIMAL NUMBER: C93216 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 04/24/00 STUDY DAY OF DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 514.4 GRAMS DATE AND TIME OF NECROPSY: 04/24/00 9:36 PROSECTOR: TROY ELLENBECKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY UTERUS (UT) : -MASS(ES); WITHIN LUMEN OF RIGHT HORN: SINGLE MASS, 10 X 6 X 4 MM, RED, DARK RED, TAN, SOFT; COLLECTED INTACT; REGIONAL LN (SEE LUMBAR) LN, AXILLARY (AX) : -LARGE; RIGHT: 15 X 7 X 3 MM (LEFT IS 5 X 3 X 1 MM) (CAP 25) LN, MANDIBULAR (MN) : -LARGE; RIGHT: 10 X 5 X 2 MM (LEFT IS 5 X 3 X 1 MM) (CAP 23) LN, LUMBAR/ILIAC (LM) : -CYST(S); RIGHT: SINGLE CLEAR FLUID FILLED CYST, 2 MM IN DIAMETER (CAP 24) -LARGE; RIGHT: 10 X 5 X 2 MM (LEFT IS 7 X 3 X 1 MM) (CAP 24) PINNA (PN) : -MASS(ES); RIGHT INVOLVING ENTIRE EAR CANAL: SINGLE MASS, 12 X 10 X 6 MM, RED, DARK RED, TAN, AND FIRM; SAME ON CUT SURFACE (CAP 22); REGIONAL LN (SEE MANDIBULAR) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN UTERUS (UT) : -HYPERPLASIA, CYSTIC, ENDOMETRIAL,MODERATE CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, AXILLARY (AX) : -HYPERPLASIA, LYMPHOPLASMACYTIC,MODERATELY SEVERE LN, MANDIBULAR (MN) : -HYPERPLASIA, LYMPHOPLASMACYTIC,-SEVERE LN, LUMBAR/ILIAC (LM) : -LYMPHANGIECTASIS,-PRESENT -HYPERPLASIA, LYMPHOPLASMACYTIC,MODERATELY SEVERE PINNA (PN) : -B-PAPILLOMA, SQUAMOUS CELL,-NEOPLASM PRESENT 3988 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2227 ANIMAL NUMBER: C93216 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 04/24/00 STUDY DAY OF DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 514.4 GRAMS DATE AND TIME OF NECROPSY: 04/24/00 9:36 PROSECTOR: TROY ELLENBECKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), TAIL (TI), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOt /f OOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), THYROID (TY), PARATHYROID (PT), URINARY BLADDER (UB) 3989 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2228 ANIMAL NUMBER: C93217 SEX: FEMALE DATE OF DEATH: 01/14/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 01/14/00 9:35 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DEATH: 635 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: NOT ENTERED PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL KIDNEY (KD) : -DILATATION, PELVIC,-SLIGHT SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SLIGHT PANCREAS (PA) : -ATROPHY, ACINAR,-SLIGHT -INFILTRATION, FAT, INTERSTITIAL,MODERATE ADRENAL, CORTEX (AC) : -HYPERTROPHY, CORTICAL CELL,-SLIGHT -PIGMENT,-SLIGHT OVARY (OV) : -CORPORA LUTEA, PERSISTENT,-PRESENT MARROW, FEMUR (FM) : -HYPERPLASIA, ERYTHROID, MARROW,-SLIGHT MARROW, STERNUM (SE) : -HYPERPLASIA, ERYTHROID, MARROW,-SLIGHT 3990 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2229 ANIMAL NUMBER: C93217 SEX: FEMALE DATE OF DEATH: 01/14/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 01/14/00 9:35 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DEATH: 635 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: NOT ENTERED PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY LUNG (LU) : -LIGHT FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE TAN AREAS, UP TO 5 X 4 MM PITUITARY (PI) : -RAISED AREA(S); LEFT MID ASPECT: SINGLE TAN AREA, 3 X 3 X 3 MM THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATE LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL -INFILTRATE, MACROPHAGE, ALVEOLAR,SLIGHT PITUITARY (PI) : -HYPERPLASIA,-SLIGHT VAGINA (VA) : -HYPERPLASIA, MUCINOUS,-SLIGHT CAUSE OF DEATH (CD) : -UNDETERMINED MICROSCOPICALLY,-PRESENT 3991 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2230 ANIMAL NUMBER: C93217 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 01/14/00 STUDY DAY OF DEATH: 635 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 01/14/00 9:35 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG), GENERAL COMMENT (GC) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), HEMATO NEOPLASIA (HN) 3992 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2231 ANIMAL NUMBER: C93218 SEX: FEMALE DATE OF DEATH: 01/03/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 01/03/00 15:10 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 624 STUDY WEEK OF DEATH: 90 TERMINAL BODY WEIGHT: 458.8 GRAMS PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); AXILLARY REGION-LEFT; WEEK 50; 20 X 20MM; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; AXILLARY REGION-LEFT; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -LARGE; ALL LOBES; MARGINS APPEAR ROUNDED -MOTTLED; ALL LOBES: BROWN AND TAN KIDNEY (KD) : -GRANULAR MATERIAL; BILATERAL, WITHIN PELVIS: MULTIPLE TAN AND FIRM GRANULES, UP TO 1 MM IN DIAMETER SPLEEN (SP) : -LARGE; 70 X 20 X 15 MM HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT -HEMATOPOIESIS, EXTRAMEDULLARY,-MINIMAL -HYPERPLASIA, BILE DUCT,-SLIGHT -INFILTRATE, MACROPHAGE, PIGMENTED,SLIGHT KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SEVERE PANCREAS (PA) : -ZYMOGEN GRANULES, DECREASED,-SLIGHT ADRENAL, CORTEX (AC) : -HYPERTROPHY, CORTICAL CELL,-SLIGHT -PIGMENT,-SLIGHT MARROW, FEMUR (FM) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT -HYPERPLASIA, MYELOID, MARROW,-MODERATE 3993 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2232 ANIMAL NUMBER: C93218 SEX: FEMALE DATE OF DEATH: 01/03/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 01/03/00 15:10 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 624 STUDY WEEK OF DEATH: 90 TERMINAL BODY WEIGHT: 458.8 GRAMS PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY LUNG (LU) : -RAISED FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE DARK RED AND TAN AREAS, UP TO 4 X 3 X 3 MM PITUITARY (PI) : -MASS(ES); 7 X 7 X 5 MM, DARK RED, TAN AND SOFT MAMMARY, FEMALE (MF) : -MASS(ES); LEFT AXILLARY REGION: SINGLE MASS, 55 X 25 X 15 MM, TAN, GRAY AND FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (SEE AXILLARY); OVERLYING SKIN HAS A SINGLE DARK RED AND TAN ABRADED AREA, 30 MM IN DIAMETER; COLLECTED INTACT MARROW, FEMUR (FM) : -HYPERPLASIA, MEGAKARYOCYTIC, MARROW,MODERATE MARROW, STERNUM (SE) : -HYPERPLASIA, MYELOID, MARROW,-SLIGHT -HYPERPLASIA, MEGAKARYOCYTIC, MARROW,MODERATE THYMUS (TH) : -DEPLETION, LYMPHOCYTIC (GENERALLY COMPATIBLE WITH PHYSIOLOGIC ATROPHY),MODERATELY SEVERE LUNG (LU) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT SKIN (SK) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT -HYPERPLASIA, GLANDULAR,-MINIMAL >NOTE:>SEE SKIN FOR DIAGNOSIS OF TUMOR 3994 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2233 ANIMAL NUMBER: C93218 SEX: FEMALE DATE OF DEATH: 01/03/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 01/03/00 15:10 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 624 STUDY WEEK OF DEATH: 90 TERMINAL BODY WEIGHT: 458.8 GRAMS PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY LN, AXILLARY (AX) : -LARGE; LEFT: UP TO 15 X 10 X 7 MM (RIGHT IS UP TO 5 X 3 X 3 MM)(CAP 21) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN VAGINA (VA) : -LUMEN, EXUDATE,-PRESENT HEMATO NEOPLASIA (HN) : -M-SARCOMA, HISTIOCYTIC,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -NEOPLASM, HEMATOPOIETIC,-PRESENT LN, AXILLARY (AX) : -HYPERPLASIA, LYMPHOPLASMACYTIC,-SLIGHT 3995 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2234 ANIMAL NUMBER: C93218 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/03/00 STUDY DAY OF DEATH: 624 STUDY WEEK OF DEATH: 90 TERMINAL BODY WEIGHT: 458.8 GRAMS DATE AND TIME OF NECROPSY: 01/03/00 15:10 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS) FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL) URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV) 3996 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2235 ANIMAL NUMBER: C93219 SEX: FEMALE DATE OF DEATH: 04/24/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/24/00 9:38 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 761.8 GRAMS PROSECTOR: JAMES T. CALLEN RECORDER: JERALD W. JOHNSON PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; SORE/SCAB; VENTRALTHORACIC-RIGHT; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE DARK RED AREAS, UP TO 5 X 4 MM; COLLECTED ON ROUTINE SECTIONS KIDNEY (KD) : -LARGE PELVIS(ES); RIGHT ADRENAL, CORTEX (AC) : -LARGE; LEFT: 7 X 5 X 5 MM (RIGHT IS 4 X 3 X 2 MM) OVARY (OV) : -CYST(S); LEFT: SINGLE CLEAR FLUID FILLED CYST, 10 MM IN DIAMETER HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-SLIGHT -HYPERPLASIA, BILE DUCT,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -DILATATION, PELVIC,-SEVERE -MINERALIZATION, PELVIC,-MINIMAL ADRENAL, CORTEX (AC) : -HYPERTROPHY, CORTICAL CELL,-SEVERE -DEGENERATION, CYSTIC / HEMORRHAGIC,MODERATELY SEVERE -PIGMENT,-MINIMAL OVARY (OV) : -CYST,-PRESENT THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL STOMACH, GL (ST) : >UNREMARKABLE 3997 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2236 ANIMAL NUMBER: C93219 SEX: FEMALE DATE OF DEATH: 04/24/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/24/00 9:38 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 761.8 GRAMS PROSECTOR: JAMES T. CALLEN RECORDER: JERALD W. JOHNSON PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: SINGLE DARK BROWN FOCUS, LESS THAN 1 MM IN DIAMETER SKIN (SK) : -CRUSTED AREA(S); RIGHT VENTRAL ABDOMINAL REGION: SINGLE DRY, TAN, RED CRUSTED AREA, 6 MM IN DIAMETER (CAP 22) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -ANIMAL OBESE SKIN (SK) : -B-KERATOACANTHOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -HYPERPLASIA, GLANDULAR,-MINIMAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 3998 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2237 ANIMAL NUMBER: C93219 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 04/24/00 STUDY DAY OF DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 761.8 GRAMS DATE AND TIME OF NECROPSY: 04/24/00 9:38 PROSECTOR: JAMES T. CALLEN RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), LN, MANDIBULAR (MN), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), ADRENAL, MEDULLA (MA), LUNG (LU), PARATHYROID (PT), URINARY BLADDER (UB) 3999 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2238 ANIMAL NUMBER: C93220 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 04/24/00 STUDY DAY OF DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 580.2 GRAMS DATE AND TIME OF NECROPSY: 04/24/00 8:12 PROSECTOR: LAURIE J. SCHULLER RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); VENTRAL-ABDOMINAL; WEEK 97; 70 X 60MM; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE DARK RED AREAS, UP TO 4 MM IN DIAMETER; COLLECTED ON ROUTINE SECTIONS ADRENAL, CORTEX (AC) : -LARGE; RIGHT: 5 MM IN DIAMETER (LEFT IS 3 MM IN DIAMETER) HISTOPATHOLOGY LIVER (LI) : -INFILTRATE, LYMPHOHISTIOCYTIC,-MINIMAL -VACUOLATION, HEPATOCELLULAR, PERIPORTAL,-SLIGHT -FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL,-MINIMAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL -HYPERPLASIA, BILE DUCT,-MINIMAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL -MINERALIZATION, PELVIC,-SLIGHT -HYPERPLASIA, TRANSITIONAL CELL,-SLIGHT PANCREAS (PA) : -INFILTRATE, MACROPHAGE, PIGMENTED,SLIGHT -INFILTRATION, FAT, INTERSTITIAL,SLIGHT ADRENAL, CORTEX (AC) : -PIGMENT,-SLIGHT -DEGENERATION / NECROSIS, UNILATERAL,MODERATELY SEVERE -M-CARCINOMA,-NEOPLASM PRESENT 4000 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2239 ANIMAL NUMBER: C93220 SEX: FEMALE DATE OF DEATH: 04/24/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/24/00 8:12 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 580.2 GRAMS PROSECTOR: LAURIE J. SCHULLER RECORDER: JERALD W. JOHNSON PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY LN, MESENTERIC (MS) : -DIFFUSELY DARK; DARK GREEN LUNG (LU) : -RED FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE DARK RED AREAS, UP TO 3 X 2 MM PITUITARY (PI) : -LARGE; 6 X 5 X 3 MM; APPEARS SWOLLEN -DARK FOCUS(I)/AREA(S); DIFFUSE: MULTIPLE BROWN AREAS, UP TO 2 MM IN DIAMETER -LIGHT FOCUS(I)/AREA(S); LEFT POSTERIOR ASPECT: SINGLE WHITE FOCUS, 1 MM IN DIAMETER MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT INGUINAL REGION EXTENDING RIGHT PERINEAL REGION: SINGLE MASS, 105 X 70 X 18 MM, RED, DARK RED, TAN, FIRM AND LOBULATED; SAME ON CUT SURFACE; PORTION COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION LN, MESENTERIC (MS) : -INFILTRATE, MACROPHAGE, PIGMENTED,SLIGHT LUNG (LU) : -N-CARCINOMA,-NEOPLASM PRESENT PITUITARY (PI) : -HYPERPLASIA,-MODERATE MAMMARY, FEMALE (MF) : -CYST / GALACTOCELE,-PRESENT -B-FIBROADENOMA,- 2 NEOPLASMS PRESENT -HYPERPLASIA, GLANDULAR,-SLIGHT 4001 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2240 ANIMAL NUMBER: C93220 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 04/24/00 STUDY DAY OF DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 580.2 GRAMS DATE AND TIME OF NECROPSY: 04/24/00 8:12 PROSECTOR: LAURIE J. SCHULLER RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS #2; LEFT VENTRAL CERVICAL REGION: SINGLE MASS, 23 X 15 X 10 MM, RED, TAN AND FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (SEE MANDIBULAR) UTERUS (UT) : -THICKENED WALL; BILATERAL: DIFFUSE LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL: DARK RED; COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -MALOCCLUSION; BILATERAL: UPPER INCISORS UTERUS (UT) : >UNREMARKABLE CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MANDIBULAR (MN) : -HYPERPLASIA, LYMPHOPLASMACYTIC,MODERATE -HEMORRHAGE,-PRESENT 4002 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-183 3M T-6295 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS PAGE: 2241 ANIMAL NUMBER: C93220 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 04/24/00 STUDY DAY OF DEATH: 736 STUDY WEEK OF DEATH: 106 TERMINAL BODY WEIGHT: 580.2 GRAMS DATE AND TIME OF NECROPSY: 04/24/00 8:12 PROSECTOR: LAURIE J. SCHULLER RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), LN, AXILLARY (AX), LN, INGUINAL (IN), LN, OTHER (LN), LN, RENAL (RL), TAIL (TI), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), AUDITORY SEB GL (AS), FOOT/FOOTPAD (FP), AORTA (AO), CALVARIUM/SKULL (CA), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), CLITORAL GLAND (CL), URETER (UE), ADIPOSE TISSUE (AT), DIAPHRAGM (DP), CAVITY, THORACIC (TA), LN, TRACHEOBRON (TB), LN, POPLITEAL (PP), MUSCLE, OTHER (MO), PERICARDIAL SAC (PD), PINNA (PN), ARTERY, OTHER (AU), AORTIC ARCH (AA), LN, ILEOCECAL (IC), BILE DUCT (BD), TRIGEMINAL NERVE (TG) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: THYROID (TY), PARATHYROID (PT), URINARY BLADDER (UB) 4003 APPENDIX 7 Cell Proliferation Report Covance 6329-183 3M T-6295 Note: This appendix contains information supplied by Pathology Associates International (PAI) and has been reviewed by the Quality Assurance Unit of PAI. 4004 Covance 6329-183 3M T-6295 Pathology Associates International A Company of Science Applications International Corporation An Employee-Owned Company FINAL CELL PROLIFERATION REPORT 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS;T-6295) IN RATS COVANCE STUDY NUMBER 6329-183 PREPARED FOR: 3M TOXICOLOGY SERVICES BUILDING 220-2E-02, 3M CENTER ST. PAUL, MN 55144-1000 PREPARED BY: PATHOLOGY ASSOCIATES INTERNATIONAL 15 WORMAN'S MILL COURT, SUITE I FREDERICK, MD 21701 SEPTEMBER 15,1999 15 Worman's Mill Court, Suite I * Frederick, Maryland 21701 * (301) 663-1644 * (301) 663-8994 FAX 4005 Contents TABLE OF CONTENTS Narrative Tables Figures Signature Page Quality Assurance Statement Appendix Covance 6329-183 3M T-6295 Final Cell Proliferation Report Covance Study Number 6329-183 Section I II in IV V VI Page 1-2 4006 Covance 6329-183 3M T-6295 Final Cell Proliferation Report Covance Study Number 6329-183 CELL PROLIFERATION REPORT 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS;T-6295) IN RATS COVANCE STUDY NUMBER 6329-183 PURPOSE The purpose o f the study was to assess the chronic toxicity and carcinogenicity of the test material when adm inistered in the diet to rats for at least 104 weeks. This report, submitted by Pathology Associates International (PAI) to the study Sponsor, 3M, represents the cell proliferation findings and interpretation for Covance Study Number 6329-183 entitled " 104-Week Dietary Chronic Toxicity and Carcinogenicity Study with Perfluorooctane Sulfonic Acid Potassium Salt (PFOS; T-6295) in Rats". All aspects of the tasks associated with P A I's portion o f this study were conducted in compliance with the Environmental Protection Agency Good Laboratory Practice (GLP) Regulations as set forth in Title 40 of the US Code o f Federal Regulations, Part 792, issued November 29, 1983 (effective December 29, 1983), and any applicable amendments with the following exception. An expired reagent was used in the BrdU immunohistochemistry staining procedure (mouse IgG, Vector, lot # PK-4002, PAI No. K351, exp. 4/9/99). The staining was judged to be satisfactory; therefore, this GLP deviation does not impact the scientific validity of the study. MATERIALS AND METHODS Proliferating Cell Nuclear Antigen (PCNA) Staining and Evaluation Tissue Collection for Cell Proliferation Five animals per sex per group in groups 1 through 5 were sacrificed at weeks 4 and 14 for hepatocellular proliferation. A section of the left lateral lobe of the liver from each of 5 rats per group (groups 1-5) was fixed in zinc formalin, processed, and embedded to paraffin block by Covance per protocol specifications. Tissue blocks were shipped to PAI for sectioning and staining. From each block, a slide was prepared for H&E evaluation and immunohistochemical detection of proliferating cell nuclear antigen (PCNA), an endogenous marker of cell proliferation. Immunohistochemistry for Cell Proliferation Sections of paraffin-embedded tissues were cut at 5 pm and placed on positively charged slides (Superfrost Plus, Fisher Scientific, Pittsburgh, PA) to ensure adhesion during processing for PCNA. Standard immunohistochemical methods were used to stain tissues for PCNA [PAFs Standard Operating Procedure for Immunohistochemical Staining (SOP #707)]. Briefly, tissue sections were incubated with a monoclonal antibody to PCNA (DAKO, lot #016; PAI No. Page 1-3 4007 Covance 6329-183 3M T-6295 Final Cell Proliferation Report Covance Study Number 6329-183 A1899) and reagents required for the avidin-biotin peroxidase (ABC Kit; Vector, lot #PK-6100; PAI No. K 327) method for the detection of the antigen-antibody complex. PCNA expression in cells in all phases of the cell cycle (G,, S, G2 and M) was localized by the chrom agen 3,3'diaminobenzidine (DAB; Sigma Chemical Co., lot #18H8201). Tissue sections were counterstained with hematoxylin. Cell Proliferation Measurements The percentage of proliferating cells (proliferating index, PI) was determined by scoring at least 2000 hepatocytes in at least 6 fields of liver. In most cases, at least 3000 hepatocytes were scored in 10 fields, but in four animals there was insufficient tissue present on the slide to be able to score as many cells. Slides were first perused at low magnification (100X) to judge quality o f staining, processing and sectioning, and pattern of cell labeling (e.g., centrilobular or panlobular, extrahepatocellular proliferation, such as Kupffer cells, bile duct epithelium , endothelium ), and histomorphologic changes. The latter was further assessed by evaluating the serial H& E slide for each animal evaluated for cell proliferation. Cell proliferation was then quantified at higher magnification (200X) as described above. A negative control slide was included in the staining run and consisted of study tissue from animal #C93008 that was not incubated with the primary antibody. Statistical Analysis The Student's f-test (two-sided, unequal variance) was used to test for statistical significance in PI between control and treatment groups using Microsoft Excel version 5.0. A P value of 0.05 was judged to be statistically significant. Bromodeoxvuridine fBrdU) Staining and Evaluation Tissue Collection for Cell Proliferation Seven days before the week 52 interim sacrifice, osmotic pumps containing 20 mg/ml BrdU were surgically implanted in five animals per sex per group in groups 1 and 5 for hepatocellular proliferation. A section of the liver and duodenum from each of 5 rats per group (groups 1 and 5 only) was taken at necropsy and embedded to paraffin block by Covance per protocol specifications. Tissue blocks were shipped to PAI for sectioning and staining. From each block, a slide was prepared for H&E evaluation and immunohistochemical detection of BrdU, an exogenous marker of cell proliferation. Immunohistochemistry for Cell Proliferation Sections of paraffin-embedded tissues were cut at 5 pm and placed on positively charged slides (Superfrost Plus, Fisher Scientific, Pittsburgh, PA) to ensure adhesion during processing for BrdU. Standard immunohistochemical methods were used to stain tissues for BrdU [PAFs Standard Operating Procedure for Immunohistochemical Staining (SOP #707)]. Briefly, tissue sections were incubated with a monoclonal antibody to BrdU (Becton Dickenson, lot #81604; Page 1-4 4008 Covance 6329-183 3M T-6295 Final Cell Proliferation Report Covance Study Number 6329-183 PAI No. A2089) and reagents required for the avidin-biotin peroxidase (ABC Kit; Vector, lot #PK-6100; PAI No. K351) method for the detection of the antigen-antibody complex. BrdU incorporation into cells in S phase o f the cell cycle was localized by the chrom agen 3,3'diaminobenzidine (DAB; Sigma Chemical Co., lot #108H8210). Tissue sections were counterstained with hematoxylin. Cell Proliferation Measurements The percentage of BrdU-labeled cells (labeling index, LI) was determined by scoring at least 2000 hepatocytes in 10 fields of liver. Slides were first perused at low m agnification (100X) to judge quality of staining, processing and sectioning, pattern of cell labeling (e.g., centrilobular or panlobular, extrahepatocellular proliferation, such as Kupffer cells, bile duct epithelium, endothelium), and histomorphologic changes. The latter was further assessed by evaluating the H&E slide for each animal evaluated for cell proliferation. Cell proliferation was then quantified at higher magnification (200X) as described above. A negative control slide was included in the staining run and consisted of study tissue from animal #C92534 that was not incubated with the primary antibody. Statistical Analysis The Student's i-test (two-sided, unequal variance) was used to test for statistical significance in L I between control and treatment groups using Microsoft Excel version 5.0. A P value of 0.05 was judged to be statistically significant. RESULTS PCNA Cell Proliferation Individual animal and group mean PCNA cell proliferation data are presented in Section II (Tables 1-4) and graphically in Section III (Figures 1 and 2). The negative control tissue was judged to be free from background staining. At 4 and 14 weeks on study, the percentage of proliferating hepatocytes (PI) in treated animals did not differ significantly from control animals for either sex. BrdU Cell Proliferation Individual animal and group mean BrdU cell proliferation data are presented in Section II (Table 5) and graphically in Section III (Figure 3). The negative control tissue was judged to be free from background staining. The percentage of labeled hepatocytes (LI) in treated animal groups did not differ significantly from control animals for either sex at 52 weeks on study. Histopathology Sections from the same tissue blocks used for preparation of PCNA- and BrdU-stained slides were stained with hematoxylin and eosin (H&E) for histopathologic evaluation to facilitate the Page 1-5 4009 Covance 6329-183 3M T-6295 Final Cell Proliferation Report Covance Study Number 6329-183 interpretation of the immunostained slides. Individual animal findings and group summaries are presented in Appendix I. The results showed that no significant changes were observed in the liver from male and female rats, which would alter the interpretation of the PCNA or BrdU staining in this study. DISCUSSION In the present study, cell proliferation was measured by PCNA im m unohistochem istiy within the liver of male and female rats from control (Group 1), low dose (Group 2), mid dose (Group 3), mid-high dose (Group 4), and high dose (Group 5) groups after 4 and 14 weeks on study, and by BrdU immunohistochemistry in the liver from control (Group 1) and high dose (Group 5) m ale and female rats after 52 weeks on study. The decision to use BrdU labeling to detect proliferating cells at 52 weeks was based on the increased sensitivity o f continuous labeling with BrdU compared to PCNA in detecting a proliferative response in the liver of rats. In this case, BrdU was administered via osmotic pump continuously for 7 days. A statistically significant increase in cell proliferation, as determined by the PCNA proliferating index (PI) at 4 and 14 weeks, or by the BrdU labeling index at 52 weeks, was not observed in the liver of m ale or female rats. Although statistical analysis did not reveal a significant cell proliferative response to the test material, biological significance was evident in individual animals. Biological significance for an individual animal is defined as a doubling in PI or L I over controls and greater than the highest control value. Thus, the data are presented in terms o f severity (fold increase over controls) and incidence (number of animals showing biological significance). As seen in Text Table 1, biologically significant cell proliferation was evident in 2/5 male rats at the high dose after 4 weeks on study and in 1/5 m ale rats at the mid-high dose and mid dose after 4 weeks on study. This apparent and minimal proliferative response seen in male rats at 4 weeks was less than the control PI at 14 weeks, and was not sustained as evidenced by the lack o f a response at week 14. Furthermore, none of the anim als exhibited a proliferative response at 52 weeks. Therefore, the proliferative responses seen in these individual male animals was a possible mild response evident only at 4 weeks. Text Table 1. Biologically Significant Cell Proliferation in L iver o f Male Rats Group Week 4 Week 14 Week 52 Severity1 Incidence2 Severity1 Incidence2 Severity1 Incidence2 Low dose <1 0/5 <1 0/5 Mid dose 1.6 1/5 <1 0/5 Mid-high dose 1 .0 1/5 <1 0/5 High dose 1.5 2/5 <1 0/5 ---- <1 0/4 'Fold increase over controls 2Number of animals with a PI or LI double the mean control value and greater than the highest control animal Page 1-6 4010 Covance 6329-183 3M T-6295 Final Cell Proliferation Report Covance Study Number 6329-183 In contrast to male rats, biologically significant cell proliferation was evident in female rats at the mid-high dose (2/5 rats) and at the high dose (2/5 rats) after 4 weeks, and at the low dose (2/5 rats) and mid dose (1/5 rats) after 14 weeks on study, as seen in Text Table 2. The degree of the proliferative response observed in females was greater than that seen in the males. The proliferative response observed atweek 4 was not sustained after 14 weeks, nor was a proliferative response observed at 52 weeks. Based on the severity and incidence of the proliferative response seen in the high dose females, and the dose response relationship seen at 4 weeks, the test material is judged to induce a transient proliferative response in the liver of female rats at the high dose. Text Table 2. Biologically Significant Cell Proliferation in Liver of Female Rats Week 4 We ek 14 Wee k 52 Group Severity1 Incidence2 Severity1 Incidence2 Severity1 Incidence2 Low dose 1 .0 0/5 2.6 2/5 - - Mid dose 1.1 0/5 1.2 1/5 - - Mid-high dose 1.8 2/5 <1 0/5 - _ High dose 3.5 2/5 <1 0/5 <1 0/5 `Fold increase over controls 2Number of animals with a PI or LI double the mean control value and greater than the highest control animal Interestingly, a rare pattern of PCNA-labeling was observed in high dose female rats at week 14, which was not seen in any other groups, nor at the earlier time point. The staining was characterized by a granular cytoplasmic staining pattern localized to the hepatocytes around central veins of the liver. The significance of this type of labeling pattern is not known; however, it has been speculated that it may represent mitochondrial DNA synthesis (unpublished observation). Recently, it has been shown that PCNA can cross-react with peroxisomal multifunctional protein (Mayer, et al., 1998). Thus, it is possible that the centrilobular staining pattern observed in the high dose animals may be associated with peroxisomes. The cell proliferation rates in the week 14 male rats were all higher than those in the week 4 male rats. In the female rats, cell proliferation rates at week 14 were higher than at week 4 in the control, low dose and mid dose groups, but not in the mid-high or high dose groups. The reason for the consistently higher cell proliferation rates in the week 14 animals is not known. However, all slides from the 4 and 14 week time points were stained together, so variation in staining between runs can be ruled out. One possibility is that the tissues were handled differently in terms of fixation and processing, which may have affected the staining. Despite this difference, comparisons can be made between each treatment group and the control group for a specific time point. SUMMARY The cell proliferation seen in the liver of male rats at 4 weeks was not dose-related, was minimal in severity (high dose was less than two-fold greater than controls), occurred in two of five Page 1-7 4011 Covance 6329-183 3M T-6295 Final Cell Proliferation Report Covance Study Num ber 6329-183 animals in the high dose group, and was transient in nature (i.e., was not sustained at 14 w eeks). In female rats, cell proliferation in the liver was dose-related at week 4 but not at w eek 14, greater in severity com pared to male rats (greater than two-fold at the high dose), and occu rred in two of five animals in both the high and mid-high dose groups. Based on these findings, cell proliferation effects seen at 4 weeks in the rats were considered to be related to test m aterial. At 52 weeks, there was no evidence of hepatocellular proliferation in male or female rats at the high dose. LITERATURE CITED Mayer, D., Forstner, K., Beier, K., and Volkl, A. (1998). Monoclonal antibodies against proliferating cell nuclear antigen cross-react with the peroxisomal multifunctional protein. Anal Biochem 256:135-137. Page 1-8 4012 Covance 6329-183 3M T-6295 Final Cell Proliferation Report Covance Study Number 6329-183 II. TABLES Legend BrdU, bromodeoxyuridine LI, labeling index, percentage o f cells in S phase of the cell cycle as determined by BrdU staining ND, not determined PCNA, proliferating cell nuclear antigen PI, proliferating index, percentage o f cells in S, G l, G2 and M phases of the cell cycle as determined by PCNA staining SEM, standard error of the mean 4013 Covance 6329-183 3M T-6295 4014 Covance 6329-183 3M T-6295 C O V A N C E STUDY NO. 6329-183 TABLE 2. P CNA CELL PROLIFERATION IN LIVER OF FEMALE RATS AFTER 4 WEEKS O N STUDY Group 1-0 p p m PFOS (Control) 1 -0 p p m PFOS (Control) 1 -0 p p m PFOS (Control) 1 -0 p p m PFOS (Control) 1-0 p p m PFOS (Control) Sex F F F F F 2 -0.5 p p m PFOS (Low) 2 -0.5 p p m PFOS (Low) 2 -0.5 p p m PFOS (Low) 2 -0.5 p p m PFOS (Low) 2 -0.5 p p m PFOS (Low) F F F F F 3 -2.0 p p m PFOS (Mid) 3 -2.0 p p m PFOS (Mid) 3 -2.0 p p m PFOS (Mid) 3 -2.0 p p m PFOS (Mid) 3 -2.0 p p m PFOS (Mid) F F F F F 4 -5.0 p p m PFOS (Mid-High) 4 -5.0 p p m PFOS (Mid-High) 4 -5.0 p p m PFOS (Mid-High) 4 -5.0 p p m PFOS (Mid-High) 4 -5.0 p p m PFOS (Mid-High) F F F F F 5 -20.0 p p m PFOS (High) 5 -20.0 p p m PFOS (High) 5 -20.0 p p m PFOS (High) 5 -20.0 p p m PFOS (High) 5 -20.0 p p m PFOS (High) F F F F F ---- PCJsra--- Animal Proliferating Number Index* C92861 0.080% C92864 0.132% C92914 0.000% C92919 ND C92926 0.000% Mean 0.053% SEM 0.032% C92941 0.027% C92945 0.056% C92950 0.027% C92969 0.110% C92982 0.053% Mean 0.055% SEM 0.015% C92996 0.054% C93006 0.053% C93008 0.108% C93031 0.028% C93045 0.054% Mean 0.059% SEM 0.013% C93058 0.186% C93067 0.161% C93070 0.027% C93075 0.000% C93084 0.110% Mean 0.097% SEM 0.036% C93114 0.056% C93123 0.027% C93142 0.349% C93144 0.430% C93159 0.054% Mean 0.183% SEM 0.085% 'Proliferatingcellnuclearantigen; percentage ofcellsinGl, S,G2 and M Page 11-2 4015 Covance 6329-183 3M T-6295 4016 Covance 6329-183 3M T-6295 C O V A N C E STUDY NO. 6329-183 TABLE 4. PCNA CELL PROLIFERATION IN LIVER OF FEMALE RATS AFTER 14 WEEKS O N STUDY Group 1 -0 p p m PFOS (Control) 1 -0 p p m PFOS (Control) 1 -0 p p m PFOS (Control) 1 -0 p p m PFOS (Control) 1-0 p p m PFOS (Control) Sex F F F F F 2-0.5 p p m PFOS (Low) 2 -0.5 p p m PFOS (Low) 2 -0.5 p p m PFOS (Low) 2 -0.5 p p m PFOS (Low) 2 -0.5 p p m PFOS (Low) F F F F F 3 -2.0 p p m PFOS (Mid) 3 -2.0 p p m PFOS (Mid) 3 -2.0 p p m PFOS (Mid) 3 -2.0 p p m PFOS (Mid) 3 -2.0 p p m PFOS (Mid) F F F F F 4 -5.0 p p m PFOS (Mid-High) 4 -5.0 p p m PFOS (Mid-High) 4 -5.0 p p m PFOS (Mid-High) 4 -5,0 p p m PFOS (Mid-High) 4 -5.0 p p m PFOS (Mid-High) F F F F F 5 -20.0 p p m PFOS (High) 5 -20.0 p p m PFOS (High) 5 -20.0 p p m PFOS (High) 5 -20.0 p p m PFOS (High) 5 -20.0 p p m PFOS (High) F F F F F ----p e n s -- Animal Proliferating Number index* C92880 0.082% C92887 0.161% C92898 0.137% C92903 0.028% C92905 Mean SEM C92944 0.085% 0.099% 0.023% 0.110% C92962 C92967 C92978 C92987 Mean SEM C 92993 0.826% 0.215% 0.080% 0.054% 0.257% 0.145% 0.081% C93000 0.054% C93018 0.054% C93023 0.356% C93035 Mean SEM C93051 0.053% 0.120% 0.059% 0.054% C93064 0.082% C93071 0.057% C93085 0.027% C93109 Mean SEM C93111 C93127 0.053% 0.055% 0.009% 0.028% 0.140% C9343 0.081% C93155 0.000% C93169 Mean SEM 0.084% 0.067% 0.024% 'Proliferatingcellnuclear antigen; percentage ofcellsinGl. S,G2 and M Page 11-4 4017 Covance 6329-183 3M T-6295 C O V A N C E STUDY NO. 6329-183 TABLE 5. BRDU CELL PROLIFERATION IN RAT LIVER AFTER 52 WEEKS O N STUDY Treatment Group 1 1 1 1 1 Gender Male Male Male Male Male 5 Male 5 Male 5 Male 5 Male 1 Female 1 Female 1 Female 1 Female 1 Female 5 Female 5 Female 5 Female 5 Female 5 Female Animal Number C92504 C92512 C92534 C92562 C92570 Mean SEM C92778 C92795 C92796 C92817 Mean SEM C92876 C92889 C92899 C92923 C92930 Mean SEM C93112 C93120 C93154 C93163 C93171 Mean SEM ------------- Brdu------------Labeling Index* 0.51% 0.19% 0,09% 0.26% 0.24% 0.26% 0.07% 0.08% 0.31% 0.09% 0.38% 0.22% 0.08% 6.94% 1.23% 1.64% 1.40% 0.11% 2.26% 1.20% 0.26% 0.11% 0.03% 0.27% 0.13% 0.16% 0.05% *Bromodeoxyuridine; percentage of cells in S phase Page 11-5 4018 PROLIFERATING INDEX (%) Covance 6329-183 3M T-6295 4019 PROLIFERATING INDEX (% ) Covance 6329-183 3M T-6295 4020 Covance 6329-183 3M T-6295 4021 Covance 6329-183 3M T-6295 Final Cell Proliferation Report Covance Study Number 6329-183 V. QUALITY ASSURANCE STATEMENT 4022 cn oo Gin \ Pathology Associates International A Company o f Science Applications International Corporation Covance 6329-1 3M T-62 An Employee-Owned Company Cell Proliferation Report 104-W eek Dietary Chronic Toxicity and Carcinogenicity Study With Perfluorooctane Sulfonic Acid Potassium Salt (PFOS; T-6295) in Rats Covance Study No,: 6329-183 QUALITY ASSURANCE STATEMENT This cell proliferation project has been inspected and audited by the PAI Quality Assurance Unit (QAU) as required by the Good Laboratory Practice (GLP) regulations promulgated by the U. S. Environmental Protection Agency (EPA-TSCA). The cell proliferation report is an accurate reflection of the recorded data. The following table is a record of the inspections/audits performed and reported by the QAU. Date of Inspection Phase Inspected Date Findings Report to PAI Management/ Project Manager/Pathologist 08/18/98 10/07/98 10/07/98 05/10/99 08/20,24/99 09/15/99 Labeling (100% slide/block match) Study Data and Supporting Documentation Draft Cell Proliferation Report Staining and Coverslipping Second Draft Cell Proliferation Report Final Cell Proliferation Report 08/18/98 10/08/98 10/08/98 05/10/99 08/24/99 09/15/99 Karen E. Butler Quality Assurance Specialist 15 Worman's Mill Court, Suite I Frederick, Maryland 21701 (301) 663-1644 (301) 663-8994 FAX 4023 Covance 6329-183 3M T-6295 Final Cell Proliferation Report Covance Study Number 6329-183 VI. APPENDIX 4024 Covance 6329-183 3M T-6295 Covance Study Number 6329-183 Individual Animai Findings 104-W eek Dietary Chronic Toxicity and Carcinogenicity Study w ith Perfluorooctane Sulfonic Acid Potassium Salt (PFOS;T-3295) in R ats H istopathologic Findings after 4 W eeks on Study Tissue: Liver A nim al N um ber C92506 C92524 C92526 C92529 C92546 C92574 C92575 C92610 C92613 C92618 C92646 C92650 C92652 C92668 C92678 C92715 C92718 C92731 C92734 C92744 C92752 C92759 C92779 C92793 C92798 S ex Dose Group M 1-0 ppm PFOS (Control) M 1-0 ppm PFOS (Control) M 1-0 ppm PFOS (Control) M 1-0 ppm PFOS (Control) M 1-0 ppm PFOS (Control) M 2-0.5 ppm PFOS (Low) M 2-0.5 ppm PFOS (Low) M 2-0.5 ppm PFOS (Low) M 2-0.5 ppm PFOS (Low) M 2-0.5 ppm PFOS (Low) M 3-2.0 ppm PFOS (Mid) M 3-2.0 ppm PFOS (Mid) M 3-2.0 ppm PFOS (Mid) M 3-2.0 ppm PFOS (Mid) M 3-2.0 ppm PFOS (Mid) M 4-5.0 ppm PFOS (Mid-high) M 4-5.0 ppm PFOS (Mid-high) M 4-5.0 ppm PFOS (Mid-high) M 4-5.0 ppm PFOS (Mid-high) M 4-5.0 ppm PFOS (Mid-high) M 5-20.0 ppm PFOS (High) M 5-20.0 ppm PFOS (High) M 5-20.0 ppm PFOS (High) M 5-20.0 ppm PFOS (High) M 5-20.0 ppm PFOS (High) H istologic F indings L iv er No Significant Findings No Significant Findings No Significant Findings No Significant Findings No Significant Findings No Significant Findings No Significant Findings No Significant Findings No Significant Findings No Significant Findings No Significant Findings No Significant Findings No Significant Findings No Significant Findings No Significant Findings No Significant Findings No Significant Findings No Significant Findings No Significant Findings No Significant Findings No Significant Findings No Significant Findings No Significant Findings No Significant Findings No Significant Findings Page V I-1 4025 Covance 6329-183 3M T-6295 Covance Study Number 6329-183 Individual Animal Findings 104-W eek Dietary Chronic Toxicity and Carcinogenicity Study with Perfluorooctane Sulfonic Acid Potassium Salt (PFO S;T-3295) in Rats Histopathologic Findings after 4 Weeks on Study Tissue: Liver A n im al N um ber C92861 C92864 C92914 C92919 C92926 C92941 C92945 C92950 C92969 C92982 C92996 C93006 C93008 C93031 C93045 C93058 C93067 C93070 C93075 C93084 C93114 C93123 C93142 C93144 C93159 S ex Dose Group F 1-0 ppm PFOS (Control) F 1-0 ppm PFOS (Control) F 1-0 ppm PFOS (Control) F 1-0 ppm PFOS (Control) F 1-0 ppm PFOS (Control) F 2-0.5 ppm PFOS (Low) F 2-0.5 ppm PFOS (Low) F 2-0.5 ppm PFOS (Low) F 2-0.5 ppm PFOS (Low) F 2-0.5 ppm PFOS (Low) F 3-2.0 ppm PFOS (Mid) F 3-2.0 ppm PFOS (Mid) F 3-2.0 ppm PFOS (Mid) F 3-2.0 ppm PFOS (Mid) F 3-2.0 ppm PFOS (Mid) F 4-5.0 ppm PFOS (Mid-high) F 4-5.0 ppm PFOS (Mid-high) F 4-5.0 ppm PFOS (Mid-high) F 4-5.0 ppm PFOS (Mid-high) F 4-5.0 ppm PFOS (Mid-high) F 5-20.0 ppm PFOS (High) F 5-20.0 ppm PFOS (High) F 5-20.0 ppm PFOS (High) F 5-20.0 ppm PFOS (High) F 5-20.0 ppm PFOS (High) H istologic Findings L iv er No Significant Findings No Significant Findings No Significant Findings No Significant Findings No Significant Findings No Significant Findings No Significant Findings No Significant Findings No Significant Findings No Significant Findings No Significant Findings No Significant Findings No Significant Findings No Significant Findings No Significant Findings No Significant Findings No Significant Findings No Significant Findings No Significant Findings No Significant Findings No Significant Findings No Significant Findings No Significant Findings No Significant Findings No Significant Findings Page VI-2 4026 Covance 6329-183 3M T-6295 Covance Study Number 6329-183 Individual Animal Findings 104-Week D ietary C hronic Toxicity and Carcinogenicity Study w ith Perfluorooctane Sulfonic Acid Potassium Salt (PFO S;T-3295) in R ats H istopathologic Findings after 14 W eeks on Study Tissue: Liver A n im al N um ber C92509 C92511 C92521 C92528 C92532 C92593 C92600 C92616 C92621 C92627 C92640 C92645 C92662 C92676 C92684 C92713 -C92714 C92719 C92722 C92728 C92765 C92777 C92789 C92799 C92812 S ex Dose Group M 1-0 ppm PFOS (Control) M 1-0 ppm PFOS (Control) M 1-0 ppm PFOS (Control) M 1-0 ppm PFOS (Control) M 1-0 ppm PFOS (Control) M 2-0.5 ppm PFOS (Low) M 2-0.5 ppm PFOS (Low) M 2-0.5 ppm PFOS (Low) M 2-0.5 ppm PFOS (Low) M 2-0.5 ppm PFOS (Low) M 3-2.0 ppm PFOS (Mid) M 3-2.0 ppm PFOS (Mid) M 3-2.0 ppm PFOS (Mid) M 3-2.0 ppm PFOS (Mid) M 3-2.0 ppm PFOS (Mid) M 4-5.0 ppm PFOS (Mid-high) M 4-5.0 ppm PFOS (Mid-high) M 4-5.0 ppm PFOS (Mid-high) M 4-5.0 ppm PFOS (Mid-high) M 4-5.0 ppm PFOS (Mid-high) M 5-20.0 ppm PFOS (High) M 5-20.0 ppm PFOS (High) M 5-20.0 ppm PFOS (High) M 5-20.0 ppm PFOS (High) M 5-20.0 ppm PFOS (High) H istologic F indings Liver No Significant Findings No Significant Findings No Significant Findings No Significant Findings No Significant Findings No Significant Findings No Significant Findings No Significant Findings No Significant Findings No Significant Findings No Significant Findings No Significant Findings No Significant Findings No Significant Findings No Significant Findings No Significant Findings No Significant Findings No Significant Findings No Significant Findings No Significant Findings No Significant Findings No Significant Findings No Significant Findings No Significant Findings N o Significant Findings Page VI-3 4027 Covance 6329-183 3M T-6295 Covance Study Number 6329-183 Individual Animal Findings 104-W eek Dietary Chronic Toxicity and C arcinogenicity Study with Perfluorooctane Sulfonic A cid Potassium Salt (PFO S;T-3295) in Rats H istopathologic Findings after 14 W eeks on Study Tissue: Liver Anim al N um ber C92880 C 92887 C92898 C 92903 C 95905 C92944 C 92962 C 92967 C 92978 C 92987 C92993 C 93000 C93018 C93023 C93035 C93051 C 93064 C93071 C93085 C93109 C93111 C93127 C93143 C93155 C93169 Sex I Dose Group F 1-0 ppm PFOS (Control) F 1-0 ppm PFOS (Control) F 1-0 ppm PFOS (Control) F 1-0 ppm PFOS (Control) F 1-0 ppm PFOS (Control) F 2-0.5 ppm PFOS (Low) F 2-0.5 ppm PFOS (Low) F 2-0.5 ppm PFOS (Low) F 2-0.5 ppm PFOS (Low) F 2-0.5 ppm PFOS (Low) F 3-2.0 ppm PFOS (Mid) F 3-2.0 ppm PFOS (Mid) F 3-2.0 ppm PFOS (Mid) F 3-2.0 ppm PFOS (Mid) F 3-2.0 ppm PFOS (Mid) F 4-5.0 ppm PFOS (Mid-high) F 4-5.0 ppm PFOS (Mid-high) F 4-5.0 ppm PFOS (Mid-high) F 4-5.0 ppm PFOS (Mid-high) F 4-5.0 ppm PFOS (Mid-high) F 5-20.0 ppm PFOS (High) F 5-20.0 ppm PFOS (High) F 5-20.0 ppm PFOS (High) F 5-20.0 ppm PFOS (High) F 5-20.0 ppm PFOS (High) H istologic Findings L iv er No Significant Findings No Significant Findings No Significant Findings No Significant Findings No Significant Findings No Significant Findings No Significant Findings No Significant Findings No Significant Findings No Significant Findings No Significant Findings No Significant Findings No Significant Findings No Significant Findings No Significant Findings No Significant Findings No Significant Findings No Significant Findings No Significant Findings No Significant Findings No Significant Findings No Significant Findings No Significant Findings No Significant Findings No Significant Findings Page VI-4 4028 APPENDIX 8 Electron Microscopy Report Covance 6329-183 3M T-6295 Note: This appendix contains information supplied by the Pathology Associates International and has been reviewed by the Quality Assurance Unit of Pathology Associates International. 4029 Covance 6329-183 3M T-6295 4030 Covance 6329-183 3M T-6295 4031 TABLE OF CONTENTS Pathology Narrative Transmission Electron Micrograph Interpretations Transmission Electron Micrographs Quality Assurance Statement Covance 6329-183 3M T-6295 Section I II III IV 4032 SECTION I PATHOLOGY NARRATIVE Covance 6329-183 3M T-6295 4033 Covance 6329-183 3M T-6295 Summary T en CrL:CD (SD) IGS B R rats (five males and five females) w ere treated for 104 w eeks w ith 20.0ppm (PFOS; T-6295). Ten other rats o f similar strain (five males and five females) w ere used as controls. Liver samples w ere obtained from each o f the 20 rats during necropsy at terminal sacrifice for examination by transm ission electron m icroscopy (TEM ). Liver samples w ere assessed for ultrastructural changes that m ay be related to chronic toxicity o f the test m aterial w hen administered in the diet. E lectron m icroscopic evaluation identified mild to m oderate changes related to treatm ent. M ild to m oderate sm ooth endoplasmic reticulum hyperplasia and minimal to mild hepatocellular hypertrophy w ere the prominent features found to be different between the high dose group and control animals. O ther changes included a slight increase in the am ount o f glycogen in treated animals com pared w ith controls. Procedure The objective o f this study w as to examine ultrastructurally samples o f liver from 10 rats trea ted orally for 104-weeks w ith PFO S; T-6295. This study is p art o f a larger chronic toxicity study o f PFOS; T-6295. This portion o f the study included 10 treated rats (5 m ales and 5 females) and 10 control rats (5 males and 5 females). T reated rats w ere orally dosed daily for at least 104 w eeks w ith 20ppm o f T-6295. The control rats w ere given the basal diet only. Term inal necropsies w ere perform ed on all 20 rats. M ultiple tissues including liver w ere collected and preserved in 10% neutral-buffered formalin according to the study protocol. Additionally, samples o f liver from each animal w ere thin-sliced, placed in 2% paraform aldehyde/2.5% glutaraldehyde in 0.1M phosphate buffer then subsequently processed and embedded in epoxy blocks for examination by TEM . Thin sections (approxim ately 90nm) from the epoxy blocks w ere cut, m ounted on 200-mesh copper grids, stained w ith 5% methanolic uranyl acetate, and R eynold's lead citrate, then exam ined o n a Z eiss E M 10C o r a Zeiss 900 transm ission electron m icroscope. Both centrilobular and periportal hepatocytes w ere examined. Representative ultrastructural photom icrographs from each liver sample were taken and significant features w ere described and recorded for each micrograph, animal, and group. The descriptive features o f each rat w ere recorded on a transmission electron m icrograph interpretation form. The number o f peroxisom es per hepatocyte was manually counted then averaged for each animal. The average num ber o f peroxisom es per group w as tabulated. Results M icrograph interpretations for each rat can be found in section II. R epresentative m icrographs o f specific features are show n in section III as report figures. All remaining electron m icrographs are separately attached to this report grouped according to rat number. TEM evaluation A to ta l o f 113 m icrographs w e re evaluated. Fifty-four (54) m icrographs represented the control group while fifty-nine (59) micrographs represented the high-dose treated group. U ltrastructural findings fo r each group are sum m arized in Table 1. T here was a minimal to mild increase in the size o f hepatocytes in the treated group. Similarly, a mild to m oderate increase in sm ooth endoplasm ic reticulum (SER) w as also noted. The increase in the size o f most hepatocytes w as generally due to the increase in SER. SER is invariably affected during some types o f hepatic toxicity, mostly toxicities that induce 4034 Covance 6329-183 3M T-6295 hepatic microsomal enzymes and lipid peroxidation (Cheville, 1994). Similarly, the increase in SER is regarded as an adaptive response by animals as a means to handle drug toxicities (Ghadially, 1997). Based on personal communication with the study pathologist, liver weights w ere increased and hepatocellular hypertrophy was evident in high dose animals, which is consistent w ith ultrastructurai findings o f an increase in S E R (Ghadially, 1997). O ther minor features between treated and control groups w ere slight increases in glycogen and lipid. B oth glycogen and lipid accumulations are considered a non deleterious adaptive change and not overtly related to treatment. An incidental finding o f large granular cell leukemia was seen in rat C92775, a high dose group male. All control animals w ere considered normal. Although a small number o f control rats had a mild increase in SER, the increase in SER in hepatocytes o f treated animals was m uch m ore prominent com pared with the amount in hepatocytes o f control animals. Table 2 summarizes the quantitation o f peroxisomes for each group. Often, an increase in the number o f peroxisom es indicates an alteration in liver cell physiology. However, in this study, the average number o f peroxisomes was not obviously different between treated and untreated animals. Table 1 Ultrastructural findings per animal Animal GroupVSex2 Comments no C92505 1/M Normal hepatocytes. C92510 1/M Normal hepatocytes. C92513 1/M Normal hepatocytes. C92514 1/M Normal hepatocytes. C92518 1/M Normal hepatocytes. C92757 5/M SER is increased in 5/5 hepatocytes. One hepatocyte had scattered glycogen while another hepatocyte is hypertrophic. C92762 5/M Mild Hepatocellular hypertrophy (2/5), increased SER (5/6). Two hepatocytes had some accumulation of glycogen. C92775 5/M Circulating lymphocytes are consistent with a diagnosis of large granular cell leukemia. SER is noticeably increased in 6/6 hepatocytes. Mild hepatocellular hypertrophy (1/6). C92786 5/M Hepatocytes (5/5) had early autolytic changes most likely due to fixation artifact; therefore, interpretation was limited. C92791 5/M A moderate to marked increase in SER. Glycogen is observed in 4/5. C92868 1/F Normal hepatocytes. One hepatocyte had abundant SER while two others have mild SER accumulation. C92871 1/F Normal hepatocytes. C92874 1/F Normal hepatocytes. One hepatocyte had a small amount of SER. C92896 1/F Normal hepatocytes. SER is minimally increased. C92897 1/F Normal hepatocytes. C93113 5/F Mild hepatocellular hypertrophy (2/6). SER is increased in 3/6 hepatocytes while lipid is prominent in 2/6 hepatocytes. C93124 C93125 5/F Mitochondria appear slightly larger in 4/6 hepatocytes. 5/F Hepatocellular hypertrophy (4/6). SER is increased in 5/6 hepatocytes. One hepatocytes has scattered deposits of glycogen. C93135 5/F Mild hepatocellular hypertrophy (3/5). The amount of SER is increased in 5/5 hepatocytes while 4/5 hepatocytes have lipid accumulation. One hepatocyte also has scattered glycogen. C93149 5/F Hepatocellular hypertrophy (4/6). SER is increased in 6/6 hepatocytes. Lipid is present in 4/6 while one hepatocyte has lipofuscin granules. 1l=Control, 5=High dose groupTIM=mak7F=female; 3ratio of abnormal hepatocytes per total number of hepatocytes described. 4035 Covance 6329-183 3M T-6295 Table 2 Quantitation of Peroxisomes per rat1 Control Group Treated Animal # Male Animal # Female Animal# Male C92505 21.8 C92868 16.6 C92757 20.2 C92510 21.2 C92871 15.6 C92762 18.0 C92513 16.5 C92874 16.4 C92775 13.0 C92514 16.0 C92896 22.8 C92786 13.8 C92518 32.2 C92897 16.2 C92791 19.5 Mean = 21.6 Mean = 17.5 Mean = 16.9 Ahe average number of hepatocytes counted per rat was 4 Group Animal # Female C93113 27.5 C93125 18.3 C93135 20.0 C93149 16.3 C93124 26.8 Mean = 21.8 Conclusion Transmission electron microscopy evaluation was done on liver samples from rats treated orally with 20.0ppm PFOS;T-6295 for a total o f at least 104 weeks. Treatm ent related ultrastructural changes included minimal to mild hepatocellular hypertrophy and a mild to m oderate increase in SER. Diplomate, A.C.V.P. References Cheville NT: Ultrastructural Pathology An Introduction to Interpretation. 1st ed. p. 704 Ames: Iow a State University Press, 1994. Ghadially FN : U ltrastructural pathology o f the cell and m atrix. 3rd ed. v. 1. p. 484 London: B utterw orths, 1997 4036 SECTION n Covance 6329-183 3M T-6295 TRANSMISSION ELECTRON MICROGRAPH INTERPRETATIONS 4037 Covance 6329-183 3M T-6295 TRANSMISSION ELECTRON MICROGRAPH INTERPRETATION Study No,: 6329-183 EM 01.37 Species/Strain: Cr;CD* (SD 1GS BR Animal No.: C92505 Tissue: Liver-Centrilobular Treatment Group: 1 (Control! Block No(s).: C92505-2A Sex: Male Photo/Negative N ois).:L6116-L6121 Significant Lesions (check one): Yes X No Interpreting Pathologist (signature and date): M arch 19. 2001 Features:L6116. Liver. Hepatocyte. 7,920X. An oval shaped hepatocyte is located horizontally in the center o f the micrograph. The nucleus is round with finely granular chromatin. Within the cytosol, there are numerous lipid droplets, mitochondria, and both RER and SER. Nineteen (19) peroxisomes are counted. The micrograph also contains multiple small elongated fissures (tissue artifact). L6117. Liver. Hepatocyte. 7,920X. The hepatocyte is the exact same cell as in micrograph labeled L6I16. Peroxisomes not counted. L6118. Liver. Hepatocyte. 7,920X. A somewhat rounded hepatocyte is located in the center o f the micrograph. The nucleus o f this hepatocyte is rounded with two nucleoli, one marginating along the periphery of the nuclear membrane. Within the cytosol, there are numerous mitochondria, RER, and a number o f large lipid vacuoles. A few small lysosomes are also found. Seventeen (17) peroxisomes are counted. L6119. Liver. Hepatocyte. 7,530X. In the center o f the micrograph is a hepatocyte that has two nuclei. Both nuclei are approximately the same size and shape. Both have a centrally located nucleolus. Within the cytosol o f this large somewhat cuboidal cell are numerous mitochondria, RER, and lysosomes. Along the bottom left hand portion o f the cell, there is a myelin figure observed. Thirty (30) peroxisomes are counted. L6120. Liver. Hepatocyte. 10,200X. An oval centrally located hepatocyte is described. There is a large, round, somewhat eccentric nucleus with a single centrally located nucleolus. Within the c y to s o l, th e re a re n u m e ro u s ro u n d s a m e -s iz e d m ito c h o n d r ia , abundant RER, and a few randomly scattered lysosomes. Twenty-one (21) peroxisomes are counted. L6121. Liver. Hepatocyte. 14,400X. A high magnification of the cytoplasm of a hepatocyte. The nucleus o f the hepatocyte is found at the bottom o f the micrograph. Within the cytosol, there are a number o f mitochondria with normal lamellar cristae. In addition to the mitochondria, there is an abundance o f RER, and randomly scattered lysosomes. A bile canaliculus is observed separating this hepatocyte from another hepatocyte at the upper right hand top of the micrograph. Peroxisomes not counted. Conclusions: Normal hepatocytes. Two hepatocytes had a few lipid droplets. Peroxisome average is 21.8 per hepatocyte. THIS PAGE REVISED. 4038 Covance 6329-183 3M T-6295 TRANSMISSION ELECTRON MICROGRAPH INTERPRETATION Study No.: 6329-183 EM 01.37 Species/Strain: Cr:CD tSD) IGS BR Animal No.: C92510 Tissue: Liver-Periportal Treatment Group: 1 (Control) Block Nolsl.: C92510 2A Sex: Male Photo/Negative N o(s).:L6183-L6187 Significant Lesions (check one): Yes X No Interpreting Pathologist (signature and date): 7 ___ M arch 19, 2001 Features: L6183. Liver. Hepatocyte. 7,530X. In the center o f the micrograph is a binucleated hepatocyte. The nuclei o f this hepatocyte are similar in size and both are round. W ithin the cytosol, there are a num ber o f sim ilar sized m itochondria. RER is also observed. The cell membrane is easily delineated from the adjacent hepatocytes. Twenty (20) peroxisomes are counted. A bile canaliculus is found along the left hand edge o f the micrograph between the central hepatocyte and the adjacent hepatocyte on that side. L6184. Liver. Hepatocyte. 7,920X. A vertically centered hepatocyte is found in this micrograph. Along the top right hand comer, there are a few clumped erythrocytes. The central hepatocyte has a distinctly rounded nucleus having finely granular chromating. There are num erous mitochondria, RER, SER, and a few lipid droplets in the cytoplasm o f this cell. Twenty-two (22) peroxisomes are counted. L6185. Liver. Hepatocyte. 10,200X. A somewhat cuboidal hepatocyte is located in the center o f the micrograph. The nucleus o f this cell is small and round. The cytoplasm is abundant. N um erous mitochondria, RER, and lesser am ount o f SER are observed in the cytoplasm. Twenty-five (25) peroxisomes are counted. Erythrocytes are found along the top right hand edge o f the micrograph. L6186. Liver. Hepatocyte. 10,200X. A centrally located hepatocyte is noted in this micrograph. The nucleus is "bean" shaped due to a large lipid vacuole compressing along one side. There is another sm aller lipid vacuole also observed in the cytoplasm. In addition to the lipid, there are num erous mitochondria, and RER in the cytoplasm. Eighteen (18) peroxisomes are counted. L6187. Liver. Hepatocyte. 10,200X. A large magnification o f a single hepatocyte. The nucleus is som ewhat rounded with irregular indentation o f the nuclear membrane. A single eccentric nucleolus is also observed. The cytoplasm contains a zonal cluster o f m itochondria along the left hand edge and an expanded region o f SER along the right hand edge o f the cell. A small amount o f RER is scattered random ly throughout the cell. Twenty-one (21) peroxisomes are counted. Conclusions: Normal hepatocytes. Two hepatocytes had small lipid droplets while another had an expanded region o f SER. Peroxisome average is 21.2 per hepatocyte._____________________ THIS PAGE REVISED. COVANCE STUDY NO.: 6329-183 DATE: 4039 Covance 6329-183 3M T-6295 TRANSMISSION ELECTRON MICROGRAPH INTERPRETATION Study No.: 6329-183 EM 01.37 Species/Strain: Cr:CD ISDt IGS BR A nim al No.: C92513______ Tissue: Liver-Centrilobular Treatment Group: 1 (Control! Block No(s).: C92513-2A Sex: Male Significant Lesions (check one): _________Yes Photo/Negative No(si.:Z21345Z21349 X No Interpreting Pathologist (signature and date): ^ y March 19.2001 Features:Z21345. Liver. Hepatocyte. 15,700X. Approximately 90% o f a single hepatocyte is observed in this micrograph. The nucleus is round with marginating chromatin. Within the cytosol, there are a number o f mitochondria, SER, and RER. A few lipid vacuoles are also observed. Seventeen (17) peroxisomes are counted. Along the left hand edge o f the micrograph is a bile canaliculus. Z21346. Liver. Hepatocyte. 10,900X. In the center of the micrograph, there is a hepatocyte with a large rounded nucleus and a smaller nucleus along the right hand side of the micrograph. Within the larger nucleus, there is an eccentrically located single nucleolus. The cytosol contains a slightly decreased number of mitochondria, abundant RER, and a few randomly scattered lysosomes and lipid vacuoles. Nineteen (19) peroxisomes are counted. Z21347. Liver. Hepatocyte. 10,900X. Approximately 90% o f a single hepatocyte is found located in the center o f the micrograph. This hepatocyte has an eccentrically located nucleus with one dense nucleolus. Within the cytosol, there is a cluster o f lipid vacuoles along the right side of the cell. In addition to the lipid, there are a number o f mitochondria, abundant RER, and a small amount of SER. Twenty-one (21) peroxisomes are counted. Z21348. Liver. Hepatocyte. 15,700X. Approximately 90% o f a single hepatocyte is observed in this micrograph. The nucleus is rounded and eccentrically located in the cell. There are five lipid droplets in the cytosol surrounded by mitochondria and RER. Abundant glycogen is also noted in the cytoplasm. Nine (9) peroxisomes are counted. A bile canaliculus is located at the top o f the m icro g ra p h . Z21349. Liver. Hepatocyte. 36,200X. A high magnification of the cytoplasm of a hepatocyte. Within the cytoplasm, there are a number of normal mitochondria. Scattered between the mitochondria are RER and small glycogen rosettes. Peroxisomes not counted. Conclusions: Normal hepatocytes. A few lipid droplets were found in 4/5 hepatocytes, and scattered glycogen (2/5 hepatocytes) was also observed. Peroxisome average is 16.5 per hepatocyte.___________________ ______ __________________________________________________ 4040 Covance 6329-183 3M T-6295 TRANSMISSION ELECTRON MICROGRAPH INTERPRETATION Study No.: 6329-183 EM 01.37 Species/Strain: Cr:CD tSD IGS BR Animal No.: C92514 Tissue: Liver-Periportal Treatment Group: 1 (Control ) Block No(s).: C92514-2A Sex: Male Significant Lesions (check one): _________Yes Photo/Negative No(s).:Z21350Z21354 X No M arch 19. 2001 Features:Z21350. Liver. Hepatocyte. 10,900X. A centrally located cuboidal hepatocyte is observed in this micrograph. The nucleus is round with a marginating nucleolus. The cytosol is filled with similar-sized mitochondria, RER, and a few lysosomes. Seventeen (17) peroxisomes are counted. Along the top left hand comer, there is a portion of a sinusoid. Small bile canaliculi are found between the central hepatocyte and the adjoining hepatocytes on both sides. Z21351. Liver. Hepatocyte. 10,900X. A somewhat rounded hepatocyte is noted in the center of the micrograph. This hepatocyte contains a centrally located nucleus with a central nucleolus. The cytosol contains numerous mitochondria, RER, and a few lipid vacuoles. Eleven (11) peroxisomes are counted. Bordering the hepatocyte on the bottom and left hand side are bile canaliculi. Z21352. Liver. Hepatocyte. 10,900X. Approximately 60% o f a hepatocyte is observed in this micrograph. The nucleus is eccentrically located, round, with finely granular chromatin. There are numerous mitochondria and abundant RER in the cytoplasm. Peroxisomes not counted. Z21353. Liver. Hepatocyte. 10,900X. A cuboidal hepatocyte is located in the center o f the micrograph. The nucleus is round with a large nucleolus. The cytosol contains abundant RER, less SER, and numerous mitochondria. Twenty (20) peroxisomes are counted. A bile canaliculus is found along the lower half o f the micrograph bordering the hepatocyte. Z21354. Liver. Hepatocyte. 36,200X. A high magnification o f mitochondria in the cytoplasm o f a single hepatocyte. The mitochondria are normal with well-developed cristae. There is also abundant R E R o b served as w ell. P eroxisom es n ot counted. Conclusions: Normal hepatocytes. Peroxisome average is 16.0 per hepatocyte. 4041 Covance 6329-183 3M T-6295 TRANSMISSION ELECTRON MICROGRAPH INTERPRETATION Study No.: 6329-183 EM 01.37 Species/Strain: Cr:CD fSDI 1GS BR Animal No.: C92518 Tissue: Liver- Periportal Treatment Group: 1 (Control) Block No(s).: C92518-2A Sex: Male Significant Lesions (check one): Photo/Negative Nofs).:L6203-L6207 &Z21355 Yes X No Interpreting Pathologist (signature and date): Y March 19.2001 Features: Z21355. Liver. Hepatocyte. 10,900X. Approximately 95% o f a hepatocyte is observed in the center o f the micrograph. The hepatocyte contains an eccentrically located nucleus that has three rounded edges and a flat edge. Within the nucleus, there is a centrally located dense nucleolus. The cytosol contains numerous mitochondria, abundant RER, and less SER. Thirty-one (31) peroxisomes are counted. L6203. Liver. Hepatocyte. 7,920X. An oval hepatocyte is found in the center o f the micrograph. An eccentrically located nucleus is somewhat oval as well. The chromatin is finely granular. Within the cytosol are numerous small similar-sized mitochondria and abundant RER. A few small lysosomes are observed as well. Thirty-four (34) peroxisomes are counted. Along the bottom edge o f the micrograph, there is a portion o f a perisinusoidal fat storing cell (Ito cell). L6204. Liver. Hepatocyte. 7,920X. In the center o f the micrograph, there is a somewhat polygonal shaped hepatocyte. This hepatocyte is bordered by portions o f other hepatocytes along the bottom and top edge o f the micrograph. The nucleus is small and cuboidal in shape. There is an abundance o f cytoplasm filled with a number o f small mitochondria, SER, RER, and a few small lysosomes. Thirty-three (33) peroxisomes are counted. L6205. Liver. Hepatocyte. 7,920X. An oval shaped hepatocyte is observed in the upper central region o f the micrograph. The hepatocyte contains a large rounded nucleus having finely stippled chromatin and small rounded pale nucleolus. The cytosol contains numerous mitochondria, SER, and some RER. Thirty-six (36) peroxisomes are counted. Below this hepatocyte, there are two other hepatocytes, one filled with six large lysosomes. Another perisinusoidal fat storing cell (Ito cell) is observed along the left hand margin o f the micrograph. L6206. Liver. Hepatocyte. 10,200X. A higher magnification o f a single hepatocyte. Approximately 97% o f the hepatocyte is observed. The nucleus is large, rounded, with a somewhat centrally located nucleolus. The cytosol contains numerous mitochondria, RER, SER, and a few randomly scattered lysosomes. Twenty-seven (27) peroxisomes are counted. There is a small bile canaliculus adjacent to the hepatocyte located on the upper left hand edge of the micrograph. L6207. Liver. Hepatocyte. 28,350X. High magnification o f the cytoplasm o f a hepatocyte. Within this micrograph, there are a number of mitochondria, RER, and SER. Peroxisomes not counted.________________________________________ _______________________________________ Conclusions: Normal hepatocytes. Two hepatocytes had a small amount of SER. Peroxisome average is 32.2 per hepatocyte._____________________ _____________________________________ _ THIS PAGE REVISED. COVANCE STUDY NO.: 6329-183 4042 Covance 6329-183 3M T-6295 TRANSMISSION ELECTRON MICROGRAPH INTERPRETATION Study No.: 6329-183 EM 01,37 Species/Strain: CnCD (SD) IGS BR Animal No.: C92757 Tissue: Liver- Centrilobular Treatment Group: 5 (High-20.Oppm T-6295) Block No(s).: C92757-2A Sex: Male Significant Lesions (check one): X Yes Interpreting Pathologist (signature and date): 75/ M arch 20. 2001 Features :L6122. Liver. Hepatocyte. 7,530X . A som ewhat rounded hepatocyte is found in the center o f the micrograph. The nucleus o f this cell is small. The cytoplasm contains mitochondria that are dispersed between patches o f SER and less RER. G lycogen particles are scattered throughout the cell. Lipid vacuoles, som e with m yelin figures and a few small lysosom es are also observed. Thirty-two (32) peroxisom es are counted. Thin to som ewhat dilated sinusoids separate this hepatocyte from portions o f other hepatocytes. Similar to the central hepatocyte, lysosom es, glycogen, SER, RER, and mitochondria fill the cytoplasm o f the surrounding hepatocytes. L6123. Liver. Hepatocyte. 7,530X . In the lower center h a lf o f the micrograph som ewhat located on the right side is a large globoid hepatocyte. This hepatocyte is bordered by three other hepatocytes. One hepatocyte alon g th e lo w er left c o m e r contain s a nu m ber o f lipid vacuoles. The central hepatocyte has a small distinctly round nucleus. The cytosol is filled with widely spaced mitochondria and RER due to abundant SER. A few small lysosom es and lipid vacuoles are also observed. Twenty-two (22) peroxisom es are counted. L6124. Liver. Hepatocyte. 7,530X . The centrally located hepatocyte is oval with an eccentrically located round nucleus. The cytoplasm contains numerous mitochondria, SER, RER, and tw o large lipid vacuoles. The cell is surrounded on three sides by dilated sinusoids containing erythrocytes. Twenty (20) peroxisom es are counted. Other hepatocytes on this micrograph have similar characteristics as the hepatocyte described. The micrograph is pale and somewhat distorted due to chatter lines. L6125. Liver. Hepatocyte. 7,920X . In the center o f the micrograph, there is a large hepatocyte with no nucleus. A cluster o f lipid vacuoles are found along the right side o f the cell w hile mitochondria and SER are located in the left hand portion o f the cytoplasm . Fifteen (15) peroxisom es are counted. A number o f these peroxisomes are large and prom inent. Surrounding this cell, on three sides, is a large dilated sinusoid containing erythrocytes. L6126. Liver. Hepatocyte. 4,990X . In the center o f the micrograph is an oval hepatocyte with tw o small portions o f a nucleus. The cytoplasm is distended due to an increase in SER, numerous mitochondria, and randomly scattered RER. A few moderate sized lipid vacuoles and small lysosom es are also observed. N ineteen (19) peroxisom es are counted. Similar to hepatocyte in micrograph L6125, the hepatocyte is surrounded by dilated sinusoids that contain erythrocytes. In the top portion o f the micrograph, there is a perisinusoidal fat storing cell (Ito cell) within the sinusoid above the central hepatocyte. L6200. Liver. Hepatocyte. 7,920X . A large vertically centered oval hepatocyte is observed in this micrograph. Surrounding this hepatocytes are erythrocytes and a finely reticulated material within sinusoids. The cell has a large round nucleus with a marginating nucleolus. The cytosol o f this hepatocyte is filled with SER, RER, mitochondria, and randomly scattered variable-sized lipid vacuoles. Thirteen (13) peroxisom es are counted. Conclusions: SER is increased in 5/5 hepatocytes. One hepatocyte had scattered glycogen while another hepatocyte is hypertrophic. Peroxisom e average is 2 0.2 per hepatocyte.___________________________________ THIS PAGE REVISED. COVANCE STUDY NO.: 6329-183 4043 Covance 6329-183 3M T-6295 TRANSMISSION ELECTRON MICROGRAPH INTERPRETATION Study No.: 6329-183 EM 01.37 Species/Strain: Cr:CD (SD) IGS BR Animal No.: C92762 Tissue: Liver-Periportal Treatment Group: 5 (High-20.0ppm T-6295 ) Block No(s).: C92762-2A Sex: Male Significant Lesions (check one): X Yes Interpreting Pathologist (signature and date): M arch 20. 2001 Features: L6127. Liver. H epatocyte. 7,530X . A vertically centered oval hepatocyte is observed in this micrograph. Along the left side o f the hepatocyte, there is a dilated sinusoid containing two erythrocytes. To the right hand edge o f the micrograph, there is a small portion o f a dark staining hepatocyte. The central hepatocyte does not have a nucleus (sectioning artifact). The cytoplasm is filled with mitochondria, patches o f SER, and som e RER. A few lysosom es are also observed. D ue to the dark staining o f the micrograph, peroxisom es are not counted. L6128. Liver. H epatocyte. 7 ,530X . A pyram idal-shaped hepatocyte with a large centrally located round nucleus is located in the m iddle o f the micrograph. The cytosol o f this hepatocyte is filled with patches o f SER, RER, and numerous mitochondria. Randomly scattered lipid vacuoles and a small amount o f glycogen are also observed in the cytoplasm . Ten (10) peroxisom es are counted. A longside on the left is a sinusoid containing erythrocytes. In addition to the red blood cells, there is a large plasm a cell located in the upper left hand comer. L6129. Liver. H epatocyte. 12,100X . H igh m agnification o f the cytoplasm o f a hepatocyte. W ithin the cytoplasm, there are a number o f similar sized mitochondria, RER, and som e SER. A few randomly scattered glycogen particles and tw o lipid droplets are also observed. Peroxisom es not counted. L6130. Liver. H epatocyte. 7,5 3 0 X . A round hepatocyte is found in the center o f the micrograph. The nucleus is also round and centrally located within the cell. Randomly scattered are numerous mitochondria, RER, and a cluster o f lipid vacuoles located in the low er portion o f the cytoplasm . Surrounding lipid vacuoles is a dense accumulation o f glycogen. Thirty-one (31) peroxisom es are counted. Surrounding this hepatocyte are other hepatocytes within similar characteristics. A sinusoid containing erythrocytes is noted on the lower left hand portion o f the micrograph. L6131. Liver. H epatocyte. 7,920X . A slightly larger polygonal hepatocyte is found in the center o f the micrograph. In the upper right hand com er there is a clum p o f erythrocytes in a sinusoid. The central hepatocyte contains an eccentrically located som ewhat small, rounded nucleus with a single nucleolus. The cytoplasm contains numerous mitochondria, some RER, and a slight increase in SER. A few lipid vacuoles are also observed. T w enty-one (2 1 ) peroxisom es are counted. B e lo w this hepCatocyte, there are three other hepatocytes with similar characteristics. L6201. Liver. H epatocyte. 7,920X . A large cuboidal-shaped hepatocyte is found in the center o f the micrograph. The nucleus is eccentrically located and contains a single marginating nucleolus. The cytoplasm is distended with a large increase in SER and less RER. A moderate number o f mitochondria and a few lipid droplets are also observed in the cytoplasm . Ten (1 0 ) peroxisom es are counted. In the upper right hand com er, there is a dilated sinusoid containing one erythrocyte in a finely granular matrix. Conclusions: M ild hepatocellular hypertrophy (2/5 hepatocytes), increased SER (5/6). Two hepatocytes had som e accum ulation o f glycogen . Peroxisom e average is 18 per hepatocyte.____________________________ THIS PAGE REVISED. COVANCE STUDY NO,: 6329-183 4044 Covance 6329-183 3M T-6295 TRANSMISSION ELECTRON MICROGRAPH INTERPRETATION Study No.: 6329-183 EM 01.37 Species/Strain: CrrCD (SD IGS BR Animal No.: C92775 Tissue: Liver - Not specified Treatment Group: 5 (High-20.0ppm T-6295 ) Block No(s).: C92775-2A Sex: Male Photo/Negative No(s).:L6132-L6137 Significant Lesions (check one): X Yes Interpreting Pathologist (signature and date): March 20. 2001 Features: L6132. Liver. Hpatocyte. 10.200X. Located to the left hand side o f the m icrograph is a polygonal-shaped hepatocyte. This hepatocyte contains a centrally located nucleus with a large round nucleolus. There are a num ber o f sm all round m itochondria in the cytoplasm , separated by S E R and RER. Fifteen (15) peroxisom es are counted. Below this hepatocyte to the lower left o f the m icrograph is a sinusoid containing a num ber o f erythrocytes and four large ly m p h o cy tes. L6133. Liver. Hepatocyte. 10,200X. A pproxim ately 50% o f a hepatocyte is observed along the top o f the micrograph. This hepatocyte has a large round nucleus with finely stippled chromatin. The cytosol contains RER, m itochondria, and SER. Peroxisom es not counted. Below this hepatocyte is a sinusoid with a finely granular matrix containing a large lymphocyte and an erythrocyte. Chatter lines along the bottom half o f the micrograph are artifact. L 6134. Liver. Hepatocyte. 7.920X. A rounded hepatocyte is found in the center o f the m icrograph. This cell has a large distinctly rounded nucleus with one round nucleolus. The cytoplasm o f this cell contains abundant SER, RER, and num erous mitochondria. Fifteen (15) peroxisom es are counted. Portions o f other hepatocytes surround the central hepatocyte. These cells have similar cytoplasmic characteristics. Along the bottom o f the micrograph, a sinusoid contains two erythrocytes and a lymphocyte. L6135. Liver. Hepatocyte. 7,920X. The m icrograph is som ewhat distorted due to chatter lines. The centrally located hepatocyte is pyramidal in shape with a large rounded nucleus. The cytosol contains a num ber o f m itochondria that are separated by RER and a few small patches o f SER. Six (6) peroxisom es are counted. Another hepatocyte along the lower left hand margin o f the micrograph contains a few small lysosomes along with SER, RER and mitochondria. The nucleus o f this cell is som ewhat rounded. In the upper right hand com er, a few clum ped erythrocytes are observed along with a small tear in the tissue (artifact). L6136. Liver. Hepatocyte. 7,920X. Portions o f two hepatocytes are located in the top h alf o f the micrograph. A pproxim ately 90% o f the hepatocyte on the left is visible. The nucleus o f this cell is large and distinctly round. A som ewhat eccentrically located nucleolus is found in the nucleus. Abundant m itochondria, RER and some SER are present in the cytoplasm. Fifteen (15) peroxisom es are counted. On the bottom o f the m icrograph, there is a large sinusoid containing a circulating a lymphocyte and a few erythrocytes. L6137. Liver. Hepatocyte. 10,200X. A large hepatocyte is observed in this m icrograph. A small portion o f a sinusoid with clum ped erythrocytes is observed in the upper portion o f the m icrograph. The nucleus o f the endothelial lining o f the sinusoid is noted ju st above the upper left o f the central hepatocyte. The hepatocyte contains a large somewhat rounded nucleus with a marginating nucleolus and finely granular chromatin. The cytosol contains num erous mitochondria, RER, and large patches o f SER. A few randomly scattered dense lysosomes are also observed. Fourteen (14) peroxisomes are counted. Conclusions: Circulating lym phocytes are m ost consistent with a diagnosis o f large granular cell leukemia. SER is noticeably increased in 6/6 hepatocytes. M ild hepatocellular hypertrophy (1/6 hepatocytes). Peroxisom e average is 13.0 per hepatocyte_______________________________________________________________________________________________ 4045 Covance 6329-183 3M T-6295 TRANSMISSION ELECTRON MICROGRAPH INTERPRETATION Study No.: 6329-183 EM 01.37 S pecies/Strain: Cr:CD (SD) IGS BR Anim al No. : C92786_____ Tissue: Liver - Periportal Treatment Group: 5 (High-20.Oppm T-6295) Block No(s).: C92786-2A Sex: Male Photo/Negative N o(s).:L6106-L6610 S ignificant L esio n s (check one): _________ Y es X No Interpreting Pathologist (signature and date): M arch 20. 2Q01 Features:L6106. Liver. Hepatocyte. 7,920X. Cells in this micrograph are undergoing autolysis based on a loosely expanded, pale cytoplasm that contains fragmented and dilated endoplasmic reticulum, electron dense mitochondria with no delineated cristae and an indistinct cell membrane. The nucleus is large, round, and still intact. The morphology o f the adjoining tissue and cells is similar. In the upper left hand corner, there is a portion o f a nucleus but no cell margins. Fourteen (14) peroxisomes are counted. L6107. Liver. Hepatocyte. 7,920X. In the center o f the micrograph, there is an oval shaped hepatocyte with a large distinctly rounded nucleus. Within the cytoplasm o f this cell, there are a number o f sm a ll rounded mitochondria, RER, and two small lipid droplets (on the right hand side o f the cell). Nine (9) peroxisomes are counted. In the upper left hand corner of the micrograph adjacent to this central cell, there is a portion o f another hepatocyte with similar characteristics. L6108. Liver. Hepatocyte. 7,920X. Similar to the hepatocyte in L6106, the centrally located hepatocyte is undergoing autolysis. The cytoplasm is loosely expanded. RER and mitochondria are observed but loosely arranged. The cell membrane is indistinct. Eleven (11) peroxisomes are observed. L6109. Liver. Hepatocyte. 7,920X. Similar to the other hepatocytes, the hepatocyte in this micrograph is mildly autolytic. The cytoplasmic organelles are loosely arranged. There are a number o f mitochondria, small patches o f RER and some SER observed. The nucleus is eccentrically located, round, and small. Twenty-one (21) peroxisomes are counted. Above this hepatocyte lo c a te d in th e u p p e r le f t h a n d c o m e r o f the micrograph is a large hepatocyte with similar characteristics. L6110. Liver. Hepatocyte. 18,500X. High magnification of the cytoplasm o f a hepatocyte. The nucleus o f the cell is present in the upper left hand comer o f the micrograph. RER is somewhat dilated while the mitochondria are normal. The cell membrane along the bottom of the micrograph is difficult to discern._____ ______ ______________________________________________ Conclusions: Hepatocytes (5/5) had early autolytic changes most likely due to fixation artifact; therefore, interpretation was limited. One hepatocyte had two small lipid droplets while another had some SER. Peroxisome average is 13.8 per hepatocyte. 4046 Covance 6329-183 3M T-6295 TRANSMISSION ELECTRON MICROGRAPH INTERPRETATION Study No.: 6329-183 EM 01.37 Species/Strain: Cr:CD 1SD1IGS BR Anim al No.: C92791______ Tissue: Liver --Periportal Treatment Group: 5 (High-20.0ppm T-62951 Block No(s).: C92791-2A Sex: Male Photo/Negative NofsT:L6138-L6142 Significant Lesions (check one): X Yes _________ No 4Interpreting Pathologist (signature and date): @ M arch 20, 2001 Features:L6138. Liver. Hepatocyte. 12,100X. Approximately 90% o f an oval hepatocyte is observed in this micrograph. The hepatocyte has an eccentric nucleus located at the top o f the cell. Mitochondria appear slightly larger. There is an abundance o f SER, lesser RER, and randomly scattered glycogen particles in the cytoplasm. A few lipid vacuoles are also observed. Twelve (12) peroxisomes are counted. This hepatocyte is located adjacent to a large sinusoid, located in the upper left hand region o f the micrograph. L6139. Liver. Hepatocyte. 7,920X. A round hepatocyte is found in the center o f the micrograph. Surrounding this hepatocyte are portions of other hepatocytes. The central hepatocyte has an eccentrically located round nucleus. In the cytoplasm, numerous mitochondria are found separated by SER, RER, and a few lipid vacuoles. Randomly scattered throughout the cytoplasm are glycogen particles. Twenty (20) peroxisomes are counted. L6140. Liver. Hepatocyte. 7,920X. In the center o f the micrograph, there is a cuboidal-shaped hepatocyte. This hepatocyte has a large round nucleus with a central nucleolus. Two somewhat large lipid vacuoles and two small lipid vacuoles are observed in the cytoplasm along with numerous mitochondria, SER, and RER. Glycogen and a few lysosomes are also observed. Twenty-nine (29) peroxisomes are counted. A bile canaliculus is present above the central hepatocyte. L6141. Liver. Hepatocyte. 7,920X. A somewhat round hepatocyte is found in the center o f the micrograph surrounded on the left and bottom by other similar hepatocytes. The central hepatocyte has a ro u n d e d nucleus th a t is displaced somewhat to the right o f the cell. Organelles in the cytoplasm consist o f numerous mitochondria, SER, RER, and glycogen. A few lipid vacuoles are observed in the lower left hand portion o f the cell. Seventeen (17) peroxisomes are counted. L6142. Liver. Hepatocyte. 12,100X. High magnification o f the cytoplasm o f a hepatocyte. A large nucleus is located in the upper left hand comer while another smaller portion o f the nucleus is present just below (sectioning artifact). Within the cytoplasm, there is an increase in SER, glycogen along with the numerous mitochondria and randomly scattered RER. Peroxisomes not counted. Conclusions: A moderate to marked increase in SER. Glycogen is observed in 4/5 hepatocytes. Peroxisome average is 19,5 per hepatocyte.________________________________________________ 4047 Covance 6329-183 3M T-6295 TRANSMISSION ELECTRON MICROGRAPH INTERPRETATION Study No.: 6329-183 EM 01.37 Species/Strain: Cr:CDs fSD) IGS BR Animal No.: C92868 Tissue: Liver - Periportal Treatment Group: 1 (Controll Block No(s).: C92868 2A Sex: Female Photo/Negative No(s).:L6143-L6147 Significant Lesions (check one): Yes X___ No Interpreting Pathologist (signature and date): M arch 16. 2001 Features:L6143. Liver. Hepatocyte. 7,920X. Placed somewhat in the center o f the micrograph is a horizontally elongated hepatocyte. There is an extreme invagination o f the nucleus envelope that contains cytoplasmic organelles, mostly mitochondria and lesser RER. The remaining cytosol contains a rather small number of mitochondria, a few lipid droplets, and slight increase the amount o f SER. Fifteen ( 15) peroxisomes are counted. L6144. Liver. Hepatocyte. 7,920X. Adjacent to the central hepatocyte are other hepatocytes, some o f which contain a few large lipid vacuoles. There are two distinct hepatocytes in the center o f the micrograph. The oval-shaped hepatocyte has a centrally located normal round nucleus. Cytoplasmic organelles appear normal. Within the cytoplasm, there are a number of mitochondria, abundant RER, and a few small lysosomes. Nineteen (19) peroxisomes are counted. L6145. Liver. Hepatocyte. 7,920X. A somewhat rounded to oval hepatocyte, centrally located on the micrograph, is described. The hepatocyte nucleus is centrally located, irregularly rounded, and appears normal. There are a number o f mitochondria that are essentially the same in size and shape. Additionally within the cytoplasm, there is abundant RER, multifocal areas o f SER and a few randomly scattered lipid droplets. Fifteen (15) peroxisomes are counted. L6146. Liver. Hepatocyte. 7,920X. Along the lower right-hand com er is a small grid bar segment. A portion o f an erythrocyte is found within a sinusoid along the right aspect o f the micrograph as well. The centrally located hepatocyte is characterized by a somewhat smaller round n u c le u s with an e c c e n tr ic a lly lo c a te d small nucleolus, a n d a c y to p la s m filled with numerous mitochondria and patches of RER. The edges of the cell membrane are irregular and somewhat indistinct (fixation artifact). Seventeen (17) peroxisomes are counted. L6147. Liver. Hepatocyte. 7,920X. There is a hepatocyte vertically centered on the micrograph, a portion o f this hepatocyte is cutoff at the bottom. The hepatocyte lacks a nucleus (sectioning artifact). There are a number o f mitochondria in the cytosol along with abundant SER and lesser RER. There is a slight increase in the number o f lysosomes. Seventeen (17) peroxisomes are counted. Conclusions: Normal hepatocytes. One hepatocyte had abundant SER while two others have mild SER accumulation. A small amount o f lipid is scattered in another hepatocyte. Peroxisomes average 16.6 per hepatocyte. THIS PAGE REVISED. 4048 Covance 6329-183 3M T-6295 TRANSMISSION ELECTRON MICROGRAPH INTERPRETATION Study No.: 6329-183 EM 01.37 Species/Strain: CnCD* (SD)IGS BR Anim al No.: C92871_______ Tissue: Liver - Centrilobular Treatment Group: 1 (Control) Block No(s).: C92871 2A Sex: Female Significant Lesions (check one): Photo/Negative No(s).:L6188-L6192 L6148, L6149 Yes X No Interpreting Pathologist (signature and date): M arch 15. 2001 Features:L6188. Liver. Hepatocyte. 7,920X. A portion o f a sinusoid containing a degenerate cell is found above the centrally located hepatocyte. The hepatocyte is oval shaped. The nucleus o f the hepatocyte is round with tw o similar sized nucleoli and finely granular chromatin. Within the cytosol are numerous small, oval to elongated mitochondria and abundant RER. Nineteen (19) peroxisom es are counted. L6189. Liver. Hepatocyte. 7,920X . One whole hepatocyte and a portion o f another hepatocyte having no nucleus (sectioning artifact) are prominent in the micrograph. The whole hepatocyte is somewhat pyramidal in shape. The nucleus o f this hepatocyte is somewhat rounded and contains an eccentrically located round nucleolus. The cytosol is filled with an abundance o f RER interlacing around a moderate number o f mitochondria. There are a few lipid vacuoles and a few randomly scattered lysosom es. Eleven (11) p e ro x iso m es are counted. L6190. Liver. Hepatocyte. 7,920X . A portion o f a hepatocyte is found in the center o f the micrograph. This portion has no nucleus (sectioning artifact) but does contain numerous mitochondria, RER, and a number o f lipid droplets. Two o f the lipid droplets contain m yelin figures. A long the bottom o f the micrograph, there is a sinusoid containing clumped erythrocytes. A bove the hepatocyte, there is another sinusoid lined by a prominent endothelial cell w hile in the lumen there are clumped erythrocytes. Peroxisom es are not counted since only approximately 30% o f the hepatocyte is available for interpretation. L6191. Liver. Hepatocyte. 7,530X . A dilated sinusoid is observed along the right hand border o f the micrograph. A centrally located, polygonal hepatocyte is observed within the center and adjacent to the bile canaliculus. The nucleus o f this hepatocyte is rounded with a somewhat centrally located nucleolus. The cytosol contains abundant RER, a number o f small round to often-elongated mitochondria, and a few lysosom es. Nineteen (19) peroxisom es are counted. L6192. L iver. H epatocyte. 7,530X . Found along the right hand bottom o f the micrograph is a sinusoid containing an erythrocyte, a perisinusoidal fat storing cell (Ito cell), and serum plasma. An oval shaped hepatocyte is observed in the center o f the micrograph. The nucleus o f this cell is somewhat small compared to the cytoplasm o f the cell. The cytosol contains abundant RER, mitochondria, SER, and a few small lysosom es. Sixteen (16) peroxisom es are counted. L 6148. Liver. Hepatocyte. 12,100X. High magnification o f a single hepatocyte. The nucleus is round with finely granular chromatin. Adjacent to the nucleus is a large oval lysosom e containing reticuloparticulate material. A lso within the cytosol are a number o f mitochondria and abundant RER. Peroxisom es are not counted since the entire hepatocyte is not available for interpretation. L6149. Liver. Hepatocyte. 4,990X . An oblong hepatocyte containing abundant mitochondria and RER is located in the center o f the micrograph. Thirteen (13) peroxisom es are counted. A long one side o f the hepatocyte, there is a sinusoid containing erythrocytes and a clump o f cellular debris. In the upper right hand c o m e r is a w id e black strip e (fo ld ed tissue). Conclusions: Norm al hepatocytes. The average peroxisom es are 15.6 per hepatocyte. SIGNATURE: THIS PAGE REVISED. COVANCE STUDY NO.: 6329-183 DATE: / / / ? / / 4049 Covance 6329-183 3M T-6295 TRANSMISSION ELECTRON MICROGRAPH INTERPRETATION Study No.: 6329-183 EM 01.37 Species/Strain: Cr:CD (SD IGS BR Animal No.: C92874 Tissue: Liver --C entrilobular Treatment Group: 1 (Control) Block Nolsl.: C92874 2A Sex: Female Photo/Negative No(s).:L6150-L6154 Significant Lesions (check one): Yes X No Interpreting Pathologist (signature and date): k / M arch 15. 2001 O Features: L6150. Liver. Hepatocyte. 7,530X. A centrally located hepatocyte is bordered on all four sides by sinusoids containing erythrocytes. This hepatocyte is irregular in shape, somewhat rounded with a round central nucleus. The cytosol contains numerous mitochondria, some RER, and a few lipid droplets. Ten (10) peroxisomes are counted. L 6 1 5 I. Liver. Hepatocyte. 7,530X. A rounded hepatocyte is found in the center o f the micrograph. This hepatocyte has a distinctly round nucleus. The cytosol contains numerous mitochondria and abundant RER. A few lysosomes with reticulo-particulate material are also noted. Sixteen (16) peroxisomes are counted. A dilated sinusoid with numerous erythrocytes is found on the r ig h t h a n d s i z e a t th e t o p o f th e m ic r o g r a p h . A long the left hand margin, there is a hole (artifact). L6152. Liver. Hepatocyte. 7,530X. Two hepatocytes are found on this micrograph. One hepatocyte has a single nucleus. The other hepatocyte is binucleated. Both nuclei are rounded with one slightly bigger than the other. The large nucleus has a single nucleolus. There are numerous small mitochondria and a moderate amount o f RER. A sinusoid is noted with erythrocytes along the lower left comer and just above the binucleated hepatocyte on the upper right side o f the micrograph. Twenty-three (23) peroxisomes are counted. L6153. Liver. Hepatocyte. 4,990X. Two hepatocytes are observed in this micrograph. The one at the top edge o f the micrograph is cut almost in half. The other hepatocyte is located somewhat in the center o f the micrograph slightly to the left hand margin. This hepatocyte has an eccentrically located round n u c le u s th a t c o n ta in s f in e ly s tip p le d c h ro m a tin . T h e c y to s o l is Filled with small mitochondria, RER, and a few small lysosomes. Seventeen (17) peroxisomes are counted. L6154. Liver. Hepatocyte. 7,530X. In this micrograph, there is a single centrally located hepatocyte surrounded by portions of other hepatocytes and a sinusoid containing two small erythrocytes (along the right hand margin). The central hepatocyte has a rounded nucleus with two round nucleoli. The cytosol contains abundant RER, less SER, a mild amount of randomly scattered glycogen, and numerous small rounded mitochondria. One large lipid droplet with a myelin figure is also observed. Sixteen (16) peroxisomes are counted. Conclusions: Normal hepatocytes. One hepatocyte had a small amount o f SER while another had a few lipid droplets. Peroxisomes average 16.4 per hepatocyte. SIGNATURE: THIS PAGE REVISED. COVANCE STUDY N O DATE: 4050 Covance 6329-183 3M T-6295 TRANSMISSION ELECTRON MICROGRAPH INTERPRETATION Study No.: 6329-183 EM 01.37 Species/Strain: Cr:CD8 (SPI IGS BR Anim al N o.:C92896________ Tissue: Liver - Centrilobular Treatment Group: 1 (Control) Block No(s).: C92896 2A Sex: Female_______ Photo/Negative N o(s).:L6155-L6159 Significant Lesions (check one): Yes Interpreting Pathologist (signature and date): X No 1/ 44/ / / / ^ ^--4 M arch 16. 2001 Features:L6155. Liver. Hepatocyte. 7 ,5 3 0 X . Central to the micrograph is a hepatocyte that is somewhat pyramidal in shape. The nucleus is centrally located and round. There are two small nucleoli and finely granular chromatin within the nucleus. The cytosol contains numerous similar sized mitochondria, abundant RER, scant SER, and a few lipid droplets and lysosomes. The plasma membrane in areas is somewhat difficult to discern from adjacent hepatocytes. Twentyone (21) peroxisomes are counted. L6156. Liver. Hepatocyte. 10,200X. A higher magnification of a hepatocyte characterized by numerous mitochondria, RER, and SER. A few lipid droplets are also found. Peroxisomes not c o u n te d . L6157. Liver. Hepatocyte. 7,920X. The centrally located oval-shaped hepatocyte contains a slightly eccentrically located nucleus containing one nucleolus. The cytosol contains numerous mitochondria, RER, scant SER, and variable sized lipid droplets. Twenty-two (22) peroxisomes are counted. L6158. Liver. Hepatocyte. 7,920X. Along the right hand margin o f the micrograph there is a bile canaliculus. An erythrocyte in the upper right hand corner is within the duct. There is a rounded hepatocyte in the center o f the micrograph that contains a number o f mitochondria interwoven with patches o f RER and SER. The nucleus is slightly eccentrically located, small, and indented along one edge. A few lipid droplets are present in the right hand portion o f the cell. A particle o f debris is present in cytosol in the lower half o f the cell. Twenty (20) peroxisomes are c o u n te d . L6159. Liver. Hepatocyte. 7,920X. Centrally located on the micrograph is an oval binucleate nucleus. In one o f the nuclei, there is a small eccentrically located nucleolus. Within the cytosol, there are numerous mitochondria and RER. Randomly scattered are small patches o f SER. This hepatocyte is bordering a bile canaliculus on the lower right hand comer of the micrograph. Twenty-eight (28) peroxisomes are counted. Conclusions: Normal hepatocytes. SER is minimally increased. The peroxisome average is 22.8 per hepatocyte. 4051 Covance 6329-183 3M T-6295 TRANSMISSION ELECTRON MICROGRAPH INTERPRETATION Study No.: 6329-183 EM 01.37 Species/Strain: Cr:CD (SD IGS BR Anim al No.: C92897______ Tissue: Liver - Periportal Treatm ent Group: 1 (Control )_______ Block No(s).: C92897-2A Sex: Female Photo/Negative N o(s).:L6160-L6164 Significant Lesions (check one): _________ Yes X No Interpreting Pathologist (signature and date): M arch 19. 2001 Features: L6160. Liver. Hepatocyte. 7,920X. Two hepatocytes are observed in this micrograph. In the upper left hand comer, there is one half o f one hepatocyte. The other hepatocyte is centrally located on the micrograph. This hepatocyte is polygonal in shape with an eccentrically located nucleus. The chromatin in the nucleus is finely granular. There are numerous mitochondria and RER in the cytosol. A few randomly scattered lysosomes are also observed. Fourteen (14) peroxisomes are counted. L6161. Liver. Flepatocyte. 7,530X. A single whole hepatocyte is located in the center o f the micrograph. Surrounding this hepatocyte are portions o f other hepatocytes. There is a sinusoid along the left hand margin that contains a leukocyte. The central hepatocyte is somewhat oval with tapered ends. The nucleus is irregularly rounded with clumped chromatin and one small nucleolus. The cytosol contains numerous mitochondria and RER. A few randomly scattered lysosomes are also observed. Sixteen (16) peroxisomes are counted. L6162. Liver. Hepatocyte. 7,530X. A binucleated hepatocyte located somewhat on the left side o f the micrograph is described. The nuclei are round and similar in size. One nucleus has a small nucleolus. The cytosol is packed with mitochondria and RER. Along the upper left hand margin o f the micrograph, there is a clearing o f the hepatocytes (artifact). Seventeen (17) peroxisomes are counted. L6163. Liver. Hepatocyte. 7,530X. The micrograph is slightly dark. A single whole hepatocyte is observed in the center o f the micrograph. Cell margins are difficult to discern completely. The hepatocyte h a s a la rg e e c c e n tr ic a lly lo c a te d n u c le u s with two dense somewhat rounded nucleoli. The cytosol contains numerous mitochondria and RER. A small amount of SER, a few small lysosomes, and a few variable-sized lipid droplets. Nineteen (19) peroxisomes are counted. L6164. Liver. Hepatocyte. 10,200X. Approximately 90% o f a hepatocyte is located along the bottom central region o f the micrograph. The nucleus o f this cell is small and rounded. Chromatin is coarse. The cytosol contains numerous similar-sized mitochondria and RER. Fifteen (15) peroxisomes are counted. Two other hepatocytes are observed in the upper region of the micrograph. Conclusions: Normal Hepatocytes. One hepatocyte had a small amount o f SER and a few variable sized lipid droplets. Peroxisomes average 16.2 per hepatocyte. 4052 Covance 6329-183 3M T-6295 TRANSMISSION ELECTRON MICROGRAPH INTERPRETATION Study No.: 6329-183 EM 01.37 Species/Strain: Cr:CD tSDl IGS BR Animal No.: C93113 Tissue: Liver - Centrilobular Treatment Group: 5 (High-20.0ppm T-6295) Block No(s).: C93113-2A Sex: Female Significant Lesions (check one): X Yes Interpreting Pathologist (signature and date): M arch 19. 2001 Features: L6111. Liver. Hepatocyte. 7,920X. In the center of the micrograph, there is a cuboidal shaped slightly larger hepatocyte. The hepatocyte contains a large round nucleus with finely granular chromatin. Within the cytosol, there are a number of small round mitochondria interwoven by RER. Surrounding the nucleus and along the outer periphery o f the cell is a moderate amount o f SER. Thirty-nine (39) peroxisomes are counted. L6112. Liver. Hepatocyte. 10,200X. A cuboidal shaped hepatocyte is observed in the center of the micrograph. The nucleus is somewhat rounded with irregular indentation o f the nuclear membrane. Chromatin is clumped along the periphery o f the nucleus. There is a centrally located single nucleolus. Numerous mitochondria, RER, and lysosomes are distributed within a loosely expanded cytoplasm. Twenty-four (24) peroxisomes are counted. L6113. Liver. Hepatocyte. 7,920X. The quality o f the micrograph is poor. There is a significant amount o f lysosomes present; however, no further interpretation is done. L6114. Liver. Hepatocyte. 7,920X. Two hepatocytes are observed in this micrograph, one is located along the left hand margin while the other is adjacent to the right hand margin o f the micrograph. Approximately 90% o f the hepatocyte on the right is present. This cell has a centrally located, distinctly rounded nucleus with one round eccentric nucleolus. Numerous mitochondria, RER, some SER, and a number o f lipid vacuoles are present in the cytosol. Twenty-two (22) peroxisomes are counted. L6115. Liver. Hepatocyte. 12,100X. A high magnification o f the cytoplasm o f a hepatocyte, the nucleus o f the cell is located in upper r ig h t hand corner o f th e m ic r o g r a p h . M ito c h o n d r ia , RER, and a few lysosomes are found in the cytosol. Peroxisomes not counted. A bile canaliculus is found along the left lower edge o f the cell. L6202. Liver. Hepatocyte. 7,920X. An oval, somewhat larger hepatocyte is observed in the center o f the micrograph. The nucleus o f this cell is large, round, and contains one nucleolus. The cytosol contains numerous similar-sized mitochondria, RER, SER, and a few randomly scattered small lysosomes. Along the bottom edge o f the cells, there is a cluster o f lipid vacuoles. Twenty-five (25) peroxisomes are counted. A portion of another hepatocyte adjacent on the lower right also contains a number o f lipid vacuoles. Conclusions: Mild hepatocellular hypertrophy (2/6 hepatocytes). SER is increased in 3/6 hepatocytes while lipid is prominent in 2/6 hepatocytes. Peroxisome average is 27.5 per hepatocyte.___________ _ _ _ _ _ _ __________________________________________________________ THIS PAGE REVISED. 4053 Covance 6329-183 3M T-6295 TRANSMISSION ELECTRON MICROGRAPH INTERPRETATION Study No.: 6329-183 EM 01.37 Species/Strain: Cr:CD (SD) IGS BR Animal No.: C93124 Tissue: Liver - Centrilobular Treatment Group: 5 (High-20.Oppm T-6295) Block No(s).: C93124-2A Sex: Female Photo/Negative No(s).:L6165-L6170 Significant Lesions (check one): X Yes Interpreting Pathologist (signature and date): Features: L 6 165. Liver. Hepatocyte. 7,530X . A cuboidal-shaped bi-nucleated hepatocyte is found in the center o f the micrograph. The nuclei are similar in shape and size. Both nuclei have a centrally located nucleolus. In the cytosol, there are a number o f slightly larger round mitochondria. RER and lysosom es are also observed. T w elve (1 2 ) peroxisom es are counted. L 6166. Liver. Hepatocyte. 7,530X . In the center o f the micrograph is an oval to slightly rounded binucleated hepatocyte. The nuclei o f this cell are rounded and similar in size. One nucleus contains two nucleoli while the other has only one. The cytosol contains a number o f slightly larger mitochondria, RER, and a few clustered lysosom es. Twelve (12) peroxisom es are counted. A portion o f four other hepatocytes surrounds this hepatocyte. A large erythrocyte is found adjacent to the hepatocyte on the left hand side o f the micrograph. L6167. Liver. Hepatocyte. 7,920X . This micrograph contains portions o f two adjacent hepatocytes. Approximately 50% o f the hepatocyte on the right is present. The nucleus o f this cell is distinctly rounded, larger than normal, with a marginating nucleolus. The cytosol contains numerous mitochondria that appear slightly enlarged. RER and clustered lysosom es are also observed. Peroxisom es not counted. L 6168. Liver. H epatocyte. 7,920X . An oval hepatocyte is located horizontally in the center o f the micrograph. To the left o f this cell is a portion o f another hepatocyte. To the right o f the central hepatocyte is a sinusoid containing lipid droplets and an erythrocyte. The central hepatocyte contains a large oval nucleus with tw o slightly eccentric nucleoli. Within the cytosol, there are a number o f large round mitochondria, RER, and a large cluster o f lysosom es along the left hand edge o f the cell. In addition, individual lysosom es are found randomly scattered within the cytosol. Peroxisom es not counted. Cell borders are difficult to discern in some areas. An electron dense thin circular artifact is found on the left edge o f the m icrograph. A d d itio n a lly, there are a scattered tissue fissures over the entire micrograph (artifact). L6169. Liver. Hepatocyte. 7,920X . An oval shaped hepatocyte is found slightly to the right o f the center o f the micrograph. A large slightly rounded nucleus is located along the bottom portion o f the cell. Num erous small round tightly packed m itochondria and a large cluster o f lysosom es is found in the cytosol. The lysosom es are located adjacent to the nucleus. In addition, a small amount o f randomly scattered RER is also located in the cytoplasm . Peroxisom es are increased-sixty (60) are counted. L 6170. Liver. Hepatocyte. 7,920X . A single cuboidal hepatocyte is found in the center o f the micrograph. The nucleus is rounded with one marginating nucleolus. The cytosol is packed with small round mitochondria, lysosom es, and a small accumulation o f RER. The left edge o f the cell contains a few chatter lines (tissue artifact). Twenty-three (23) peroxisom es are counted._________________________________ Conclusions: Mitochondria appear slig h tly larger in 4 /6 h ep a to cytes. P ero x iso m e avera g e is 2 6 .8 p e r hepatocyte.______________________________________________________________________________________________ 4054 Covance 6329-183 3M T-6295 TRANSMISSION ELECTRON MICROGRAPH INTERPRETATION Study No.: 6329-183 EM 01.37 Species/Strain: Cr:CD (SD) IGS BR Anim al No.: C93125______ Tissue: Liver -Centrilobular Treatment Group: 5 (High-20.0ppm T-62951 Block No(s).: C93125-2A Sex: Female Photo/Negative No(s).:L6171-L6176 Significant Lesions (check one): X Yes Interpreting Pathologist (signature and date): Features: L6171. Liver. Hepatocyte. 7,920X . A large polygonal hepatocyte is located horizontally in the center o f the micrograph. The nucleus o f this cell is quite small while the cytoplasm is extremely distended with small round mitochondria, RER, and SER. There are a few randomly scattered small lysosom es as well. Thirty-eight (38) peroxisom es are counted. The left hand edge o f the hepatocyte contains a number o f villous projections. A small leukocyte and an erythrocyte are found along the bottom right hand margin o f the micrograph. L6172. Liver. Hepatocyte. 7,920X . A number o f hepatocytes are present in this micrograph. One cuboidal-shaped hepatocyte, vertically centered in the middle o f the micrograph is characterized by a distinctly large round eccentrically located nucleus having a rather large dense nucleolus. The cytosol o f this cell is packed with n u m ero u s sm a ll m ito c h o n d ria , R E R , and a fe w small patches o f SER. Sixteen (16) peroxisom es are counted. The hepatocyte is bordered on three sides by sinusoids that contain erythrocytes. Adjacent to the edge where the nucleus is located, there is a perisinusoidal fat storing cell (Ito cell) within a dilated sinusoid. L6173. Liver. Hepatocyte. 7,920X . A large polygonal hepatocyte is found located in the center o f the micrograph. This hepatocyte has a small nucleus compared to the cytoplasm that is greatly distended with small mitochondria, RER, and SER. Eleven (11) peroxisom es are counted. The plasma membrane contains a number o f villous projections. A number o f hepatocytes surround the central hepatocyte. These hepatocytes also contain numerous mitochondria, RER, and SER. A portion o f one hepatocyte, located along the upper right hand com er o f the micrograph, has an abundance o f glycogen. L6174. Liver. Hepatocyte. 7,920X . A cuboidal shaped hepatocyte is found located vertically in the center o f the micrograph. The left and right hand edges o f the plasma membrane contain numerous villous projections. A nucleus is n ot observed (section in g artifact). T he cyto p la sm contains large patches o f SER, RER, and a moderate number o f mitochondria. There are a few lipid vacuoles and a noticeable increase in the amount o f glycogen. Ten (10) peroxisom es are counted. B etw een this hepatocyte and the hepatocyte at the bottom o f the micrograph is a small bile canaliculus. L6175. Liver. Hepatocyte. 7,530X . A large round hepatocyte with a small portion o f the nucleus (sectioning artifact) is found in the center o f the micrograph. This cell is characterized by large numbers o f small mitochondria, abundant SER, and lesser RER. Fifteen (15) peroxisom es are counted. Surrounding this hepatocyte are portions o f other hepatocytes. L6176. Liver. Hepatocyte. 7,530X . A smaller hepatocyte is located in the center o f the micrograph surrounded by dilated sinusoids. The hepatocyte has no nucleus (sectioning artifact). Within the cytosol, there are a number o f small elongated to round mitochondria, SER, and RER. A large lipid vacuole and randomly scattered deposits o f glycogen are also observed. Twenty (20) peroxisom es are counted. In the upper right hand c o m e r o f the m icrograph is a sin u soid contain in g a sm all leu k o cy te. B elow and to the right o f the central hepatocyte, there is a small hole (sectioning artifact).___________________________________ Conclusions: Hepatocellular hypertrophy (4/6 hepatocytes). SER is increased in 5/6 hepatocytes. One hepatocyte has scattered deposits o f glycogen. Peroxisom e average is 18.3 per hepatocyte.________________ 4055 Covance 6329-183 3M T-6295 TRANSMISSION ELECTRON MICROGRAPH INTERPRETATION Study No.: 6329-183 EM 01.37 Species/Strain: Cr:CD (SD) IGS BR Anim al No.: C93135______ Tissue: Liver - Periportal Treatment Group: 5 (High-20.Qppm T-6295) Block No(s).: C93135-2A Sex: Female Photo/Negative No(s).:L6177-L6182 Significant Lesions (check one): X Yes Interpreting Pathologist (signature and date): M arch 20, 2001 Features: L 6177. Liver. Hepatocyte. 7,530X . An oval hepatocyte is found located vertically in the center o f the micrograph. A bove this hepatocyte, there is a sinusoid lined by a prominent endothelial cell that contains one small leukocyte and tw o large lipid droplets. Lipid droplets are located over the entire micrograph both within the central hepatocyte and well as other hepatocytes above and below. The central hepatocyte has a oval shaped nucleus with finely granular chromatin. The cytosol o f this cell contains numerous small round mitochondria and surrounding the nucleus is a thick zone o f RER. Small patches o f SER are also observed. Twenty-three (23) peroxisom es are counted. There is a large knife mark (artifact) running along the bottom edge o f the cell. L 6178. Liver. Hepatocyte. 3,750X . A number o f hepatocytes are observed in this micrograph. An oval hepatocyte is found vertically placed in the center o f the micrograph. The c e ll membrane h a s a n u m b e r o f villous projections especially prominent on the lower half o f the cell. The nucleus o f this cell is small and contains three nucleoli; one is distinctly round and larger than the others. Within the cytosol, there is an increase in the amount o f SER and lesser numbers o f mitochondria and RER. Eighteen (18) small peroxisom es are counted. Lipid droplets are observed along the top edge o f the cell as w ell as scattered along the bottom. Lipid droplets are found scattered throughout other hepatocytes surrounding this cell. At the top o f the micrograph, located in the center, is an electron dense, elongated trapezoid artifact. L6179. Liver. Hepatocyte. 4,990X . An oval hepatocyte is found in the center o f the micrograph. Above this hepatocyte is a sinusoid containing cellular debris and a nucleus o f a leukocyte. The central hepatocyte contains a distinctly round som ewhat eccentric nucleus. A small round eccentric nucleolus is found in the nucleus. The cytosol contains a dense population o f small mitochondria, RER, and a few patches o f SER. Twenty-tw o (22) peroxisom es are counted. A tissue fold is observed at the bottom o f the micrograph. L6180. Liver. Hepatocyte. 4 ,9 9 0 X . A polygonal hepatocyte w ith a sm all n ucleus is fo u n d in the center o f the micrograph. The hepatocyte is somewhat separated from adjacent hepatocytes by dilated sinusoids containing erythrocytes. The cytoplasm o f this cell contains a number o f thin elongated mitochondria, som e RER, and a few patches o f SER. A few small lipid droplets are also observed. Seventeen (17) peroxisom es are counted. L6181. Liver. Hepatocyte. 4,990X . The hepatocyte in this micrograph is the same one as the hepatocyte in micrograph L 6180. L6182. Liver. Hepatocyte. 7,5 30X. A higher magnification o f the cytoplasm o f a hepatocyte. The nucleus o f the hepatocyte is distinctly rounded and located in the upper left com er o f the micrograph. The cytoplasm o f this cell contains a number o f small mitochondria, RER, and numerous patches o f SER. A few small randomly scattered lipid droplets are found also within the cytosol. A few lysosom es and minute glycogen particles are similarly observed. Peroxisom es not counted.______________________________________ Conclusions: M ild hepatocellular hypertrophy (3/5 hepatocytes). The amount o f SER is increased in 5/5 hepatocytes while 4/5 hepatocytes have lipid accumulation. One hepatocyte also has scattered glycogen. Peroxisom e average is 20.0 per hepatocyte.______________________________________________________________ 4056 Covance 6329-183 3M T-6295 TRANSMISSION ELECTRON MICROGRAPH INTERPRETATION Study No.: 6329-183 EM 01.37 Species/Strain: Cr:CD (SD) IGS BR Anim al No.: C93149_____ Tissue: Liver - Periportal Treatment Group: 5 (High-20.0ppm T-6295 ) Block No(s).: C93149-2A Sex: Female Photo/Negative No(s).:L6193-L6199 Significant Lesions (check one): X Yes _______ No Interpreting Pathologist (signature and date): M arch 20, 2001 Features: L6193. Liver. Hepatocyte. 7.920X . A large som ew hat rounded hepatocyte is found located in the center o f the micrograph. The nucleus o f the cell is centrally located, distinctly rounded with slight clumping o f chromatin. The cytosol o f this cell is filled with num erous m itochondria and RER. A few small lysosomes and lipid droplets are also observed. Twelve (12) peroxisom es are counted. A portion o f another hepatocyte is observed along the bottom o f the micrograph. Within the cytoplasm o f that cell, there are a few small deposits o f lipofiiscin. L6194. Liver. Hepatocyte. 7,920X. Two oval hepatocytes are found vertically aligned in this micrograph. The bottom hepatocyte contains a large, som ewhat rounded nucleus with an eccentrically located nucleolus. W ithin the cytosol o f this hepatocyte, there are numerous mitochondria, RER, and a few large lipid vacuoles. A few randomly scattered, small, patches o f SER are also observed. Peroxisom es not counted due to darkness o f the micrograph. The hepatocytes above and below this hepatocyte also contain a num ber o f lipid vacuoles. L6195. Liver. Hepatocyte. 7,92OX. A pproxim ately 90% o f a large (fills the entire micrograph) hepatocyte is described. The nucleus is distinctly rounded and contains two large round nucleoli. The cytoplasm contains numerous small m itochondria, RER, and SER. In addition, there are num erous variable-sized lipid droplets also observed. Some o f these droplets contain debris. Twenty-one (21) peroxisomes are observed. L6196. Liver. H epatocyte. 10,200X. A pyram idal-shaped hepatocyte, located in the center o f the m icrograph, is described. This cell has an eccentrically located, rounded nucleus. The chrom atin in the nucleus is somewhat clumped along the periphery. There is a large lipid droplet in upper right hand com er o f the cell. N um erous small elongated to rounded m itochondria, abundant R ER and scattered SER are also observed in the cytoplasm. Ten (10) peroxisomes are counted. This hepatocyte is somewhat surrounded on three sides by sinusoids that are dilated and filled with cellular debris, erythrocytes, and one small leukocyte (along the upper left hand edge o f the micrograph). L6197. Liver. Hepatocyte. 10,200X. The centrally located, oval hepatocyte is som ewhat distorted (tissue artifact). The nucleus is distinctly rounded, large, with one large and one small nucleolus. The left hand portion o f the cytoplasm contains a number o f large lipid vacuoles and SER while the bottom half or right hand portion o f the cytoplasm contains clustered mitochondria and RER along with a few other lipid vacuoles. An even smaller number of lysosomes are found scattered randomly throughout the cell. Twenty-two (22) peroxisom es are counted. A large erythrocyte in a dilated sinusoid is noted along the upper right hand portion o f the m icrograph. L6I98. Liver. Blood vessel. 4,990X. There is extensive w idening o f the cytoplasm o f the endothelial cells. The nuclei o f the endothelial cells are enlarged with finely granular chromatin. Within the center o f this vessel, there is a portion o f a circulating leukocyte. Adjacent to this vessel is another large lumen (at the bottom left hand comer). The top o f the m icrograph is dark and not interpreted. L6199. Liver. Hepatocyte. 10,200X. High magnification o f the cytoplasm o f a hepatocyte. W ithin the cytosol, there are a num ber o f large lipid droplets, lipofiiscin particles, SER, RER, and m itochondria. Peroxisom es not counted.______ C onclusions: H epatocellular hypertrophy (4/6 hepatocytes). SER is increased in 6/6 hepatocytes. L ipid is present in 4/6 w hile one hepatocyte has lipofiiscin. Peroxisom e average is 16.3 per hepatocyte._________________________________ 4057 SECTION m Covance 6329-183 3M T-6295 TRANSMISSION ELECTRON MICROGRAPHS 4058 FIGURE LEGENDS Covance 6329-183 3M T-6295 Magnification. Print magnification is indicated on the back of each photograph. The Bar in the right-hand comer of the micrograph equals lmicron (0.001mm). Figure 1. Liver. Hepatocyte. This hepatocyte illustrates a few normal ultrastructural features. Within the cytosol, there are a number o f mitochondria separated by abundant RER. SER is scant to not seen in this hepatocyte. Animal no. C92871, Control Female. Figure 2. Liver. Hepatocyte. A binucleated hepatocyte located somewhat on the left side o f the micrograph also illustrates normal organelles. The cytosol is packed with small round mitochondria, and a few small aggregates of RER. Animal no. C92897, Control Female. Figure 3. Liver. Hepatocyte. A high magnification o f the cytoplasm o f a hepatocyte is illustrated. The nucleus o f the hepatocyte is found at the bottom o f the micrograph. Within the cytosol, there are a number of mitochondria with normal lamellar cristae, abundant R E R and randomly scattered lysosomes. A bile canaliculus is observed separating this hepatocyte from another hepatocyte at the upper right hand top of the micrograph. Animal no. 92505, Control Male. Figure 4. Liver. Hepatocyte. An enlarged cuboidal-shaped hepatocyte is found in the center of the micrograph. The cytoplasm is distended due to a large increase in SER and less RER. A moderate number o f mitochondria and a few lipid droplets are also observed in the cytoplasm. Animal no. C92762, High dose treated Male. Figure 5. Liver. Hepatocyte. A large polygonal hepatocyte is found in the center o f the micrograph. This hepatocyte has a small nucleus compared to the cytoplasm that is greatly distended with small mitochondria, R E R and SER. The plasma membrane contains a number of villous projections. Surrounding hepatocytes contain numerous mitochondria, R E R and SER. A portion o f one hepatocyte, located along the upper right hand comer of the micrograph, has a large amount o f glycogen. Animal no. C 93125, High dose treated Female. Figure 6. Liver. Hepatocyte. A round hepatocyte is found in the center o f the micrograph. Randomly scattered are numerous mitochondria, R E R and a cluster o f lipid vacuoles located in the lower portion o f the cytoplasm. Surrounding the lipid vacuoles is a dense accumulation of glycogen. A sinusoid containing erythrocytes is noted on the lower left hand portion o f the micrograph. Animal no. C92762, High dose treated Male. 4059 Covance 6329-183 3M T-6295 4060 Covance 6329-183 3M T-6295 4061 Covance 6329-183 3M T-6295 4062 Covance 6329-183 3M T-6295 4063 Covance 6329-183 3M T-6295 4064 Covance 6329-183 3M T-6295 4065 Abbreviations BC DC G L LY M N NU P RBC RER SER VP Covance 6329-183 3M T-6295 FIGURE LABELS FOR ULTRASTRUCTURAL STRUCTURES Structure Bile canaliculus Degenerate cell Glycogen Lipid droplet Lysosome Mitochondria Nucleus Nucleolus Peroxisomes Erythrocyte Rough endoplasmic reticulum Smooth endoplasmic reticulum Villous projection 4066 SECTION IV QUALITY ASSURANCE STATEMENT Covance 6329-183 3M T-6295 4067 Covance 6329-183 3M T-6295 4068