Document O6rx0VMMrmJVabp57aaZNJ3X
A R & A & - 0700
ACTIVATED SLUDGE RESPIRATION INHIBITION TEST
TEST SUBSTANCE __________________________________________
Identity: A mixture containing perfluorooctanesulfonate, which may also be referred to as PFOS, FC-95 or as a component of FC-201AF. (1Octanesulfonic acid) (CAS # 2795-39-3).
Remarks: The 3M production lot number was not noted. The test sample is FC-201AF. Current information indicates it is a mixture of 3% PFOS, 18.59% diethylene glycol butyl ether, 37.81% water, 14% ethylene glycol, 8.25% Acrylic foamer, 9% sodium octyl sulfate, 0.3% sodium lauryl sulfate, 3% triethanolamine, 6% sulfonated alkyl ether salts, and 0.05% tolyltriazole.
The following summary applies to a mixture with incompletely characterized concentrations o f impurities. Data may not accurately reflect the toxicity o f the fluorochemical component o f the test sample.
METHOD____________________________________________________
Method: OECD Guideline 209 Test type: Static acute GLP: No Year Completed: 1995 Analytical monitoring: Dissolved oxygen, temperature, and pH. Statistical methods: Results were determined by calculation of the % inhibition in respiration rate. Test organism source: Activated sludge mixed liquor collected from the Metro Wastewater Treatment Plant, St. Paul, MN. Test condition:
Dilution water: Millipore Milli-QTM water Synthetic sewage: per OECD method Reference and test solution preparation: A stock solution of the reference substance, 3,5-dichlorophenol, was prepared by dissolving 500 mg in 10mL 1N NaOH, diluted to 30mL with Millipore Milli-QTM water, then brought to the point of incipient precipitation with 1 N H2S 04 and diluted to 1L with Milli-QTM water. The pH of the reference solution was measured to be 7.3. Test substance was added directly to the test vessels. Test vessels: Not given. Number of concentrations: one plus blank control, in duplicate and 1 reference substance Temperature: 19.3-20.7C . Total suspended solids and pH on day of testing: 4.2 g/L (TSS in final: 1.7 g/L), initial pH 6.0 and final pH 6.5. Element Basis: Respiration inhibition as determined by oxygen consumption.
000308
Remarks: Uptake rates corrected to 20C for calculations. R E S U L T S _____________________________________________ Nominal concentrations: Blank controls and 1000 mg/L in duplicate plus one reference substance at 10 mg/L. Element values: 30-minute EC50 = >1000 mg/L
3-hour EC50 = >1000 mg/L Element values based on nominal concentrations. Remarks: Testing was conducted on a mixture as described in the Test Substance Remarks field. The values reported apply to that mixture and not the fluorochemical proportion alone. CONCLUSIONS_______________________________________________ The test substance 3-hour EC50for activated sludge respiration inhibition was determined to be >1000 mg/L. Subm itter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY_______________________________________________ R eliability: Klimisch ranking = 2. This study meets the criteria for quality testing. However, sample purity was not properly characterized and the study lacks analytical confirmation of the amount of fluorochemical proportion in the solution. REFERENCES________________________________________________ This study was conducted by the 3M Company, Environmental Laboratory, St. Paul, MN, Lab Request number N2964, 1995. OTHER______________________________________________________ Last changed: 6/26/00
000309
BEST COPY AVAILABLE
ENVIRONMENTAL LABORATORY
ACTIVATED SLUDGE MIXED UQUOR r
LR A '1 % * / Date 9 / / v 7 ? ^ Analyst G i< /S Page f of 1
: TyPe Test O E C D /}(. 1 ' >Jnie s f S / v J ^
%: :X * v . . ,
ift4, r
.\
: .* -
A
| | v `a CT1VATED SLUDGE MIXED U Q U O R
/ . '/jfJ ' /'
j> ! '{tftA J ! / # 2 0 ?
r* %
& D a te . Obtained
Date Used cC ^ / V / <f S ~
*Jg- ii
<, ,,
.
iU fiMixed Liquor Suspended Solids (MLSS1 M .2
o/Lv Adjusted to _
gA.
W s* . iM LSS in final test solution
II
on
initial p H ',: 0 . 0 (1st test soin, prepared)
' U
*' , '' f i r :V * V* ** '
:j
>: RnaTpH v> . o (last soin, prepared) ASKS?*&V*c' W*U ~ * l ff i L A f U y c r .
TE SM TW-SM Ua BM SM WTAm N* BCM WE
V.
V.. I
. . . * ,
I. S .
) Sam ple d e s c rip tio n h C ~~ 2 C / / ) f - -
*SMs' .*
* '*, * *
^ j , '
Mtis
^highest cone, tested ( t O fjlL ) - . - / : *
^^^A djusted to pH ----------
^
yj T
____- >< ' 4f ccm m *
( *mq/L)
m:pH
' ' ** *... \'w&
wJ^AR^T_-. . .. '=3'
. . .. , .' ,*/::I*'-.
: : - : ..-:.
-ki'Jxk tJL. *%
,?rii7r:i.'ic
000310
ft
BEST COPY AVAILABLE
ENVIRONMENTAL LABORATORY
OECD ACTIVATED SLUDGE RESPIRATION INHIBITION TEST #209
- -
.. -
I.. f Test Substance:
____ t v .
: Q J .A .I~
RAW DATA
Source o f Activated^ Judge Mixed Liquor: M > h-c ( 1/av h / t i h r . 'T s ra ''f n t e t j f
f f* u /
Date Received: ^ / ^ V / v
Analyst:
v-j *
&>
---s r . $ . ' )} k...
^
*V-i4Xi
A
Dale Used: ; ^
/V /* ? S ~ .
| i 30-MINUTE - EXPOSURE:
A - DISSOLVED OXYGEN CONCENTRATIONS. tag/L
' '"'Vi* .
1%> Test Substance
L)Concentration"Cf/ 0 .5 : <!*.- J / ,
A 1.5 2.0
TIME-MINUTES . 2.5 3.0 3.5
\#>ifi7i 4.0
A/!'
5.0
Temp 5.5 C
BKCONTROL1 '' ;"|Y
fn'*!* i - m*. :.\; -;Y*-?>r~ tfe-5 m%mm b-mr*v .*
57-0 G .6 6 3 f j.z 2.75
H.o-
tfet 1 - Iwss'
H it
6 .o 2 .2 2 .Z .
576
ks
l.S
5 .3 .0
S - 6 4 , 7 4.M
37
v' ? . ,vV; wSPi YYY sjistefe
>.
-'-jf-f>'-V
.r-^r* 5;
.tfc'i ;,
..>.r fcVVWtfi
BKCONTROLn. H ; i i f f l i 7 .2
m@G>
,..***cp H i #2
IQ,7
2C 6 s '
: 9>o
7 W Substance! LConcentratiorg
vj- - ..JfeBpBiti
ID1SSOLVED OXYGEJCCONCENTRAT
T IM E M IN U T E S J
2.0
Temp5
iBKCOf
k >j;
*sw*i' *(tJilSi-.
000312
BEST COPY AVAILABLE 000313
ENVIRONMENTAL LABORATORY OECD ACTIVATED SLUDGE RESPIRATION INHIBITION TEST #209 CALCULATION OF RESULTS
Test Substance: FC-201AF
Analyst: GWJ
2, f ' i r-
-
- fr
''M
km Lrti2aMl
Date:ftj
ris
m a/L 0 .5 1.0 1.5 2 .0 2 .5 3 .0 3 .5 4 .0 4 .5 5 .0 5 . 5 ' ''Temp,*C R ^ (1 )^ <Ra(2V % i|n f^ (3 )l -ir
BKV 7 .0 6.6 6.3 6.0 5.6 5.3 5.0 4 .7 4.4 4.1 3 .7 1000.0 4 .2 3.4 2 .8 2 .2 1.5 0 .9
1 9 .9 -' 0.6364* 0.6408 S!iS$gf 2 0.7 *^ 1.2600$ 1.2001 m -9 7 4Rt
Dup. 4 .0 3.3 2 .8 2 .2 1.5 1.0
20.6 * 1.1800 1.1318 m t8 6 W $
>%.?>>
BK2
8.0 7.6 7.4
% diff in controls (4): 10.2
7.2 6.9 6.6 6.3 6.1 5.8 5.4 EC50 (95% Cl): y Q C 'O IT q / L
5.0
19.8
0.5709 0.5789
. RaCm (5 ):^ 0 .6 0 9 9 |
*TMH8 "
m a/L 0 .5 1.0 1.5 2 .0 2 .5 3 .0 3 .5 4 .0 4 .5 5 .0 5 .5 Temp, *C R (D Ra (2) % Inh. (3) msmit e
BK1 7 .7 7.4 7 .2 6 .9 6 .7 6.4 6.1 5 .8 5 .5 5 .3 4 .9
19.4
0.5552 0.5788
1000.0 5.1 4 .5 4 .0 3 .6 3 .0 2 .6 2 .0 1 .6 1.0 0 .5
20.3
1.0000 0.9794 m m & B m
Dup.
6.3
5.8
5.4
4 .9
4.4
3.9
3.4
3 .0
2.5
2.0
1.5
19.9 0.9600? 0.9667
BK2 8 .3 8 .0 % diff in controls (4):
7.8 1.6
7.5 7.2 7.0 6.7 6.4 6.1 5.8 ECSO (95% Cl): y O O r n ^ J i <>*
-ci/'-'vL '..tc';..
5.5
SKWIIBi SSS
N'<
JIWIWWw
1 9 .3 ^ *0.5600% 0.5879
.RaCfn' (5):i
(t) R- Oxygenuplaka rata (mg 02/L-mln) (2) Ra* Radjustedto 20"C (R/e.x| (3) % Inhibition I(RaCm)-RaS/(RaCm)|tOO (4) %difference incontrols: (IR*Bk1-RaBk2l/RaCmj100 (Two controls must ba wkhfn 15%of aach other.) (5) Mean adjusted R, control[RaBk1*RaBk2y2
a . 1.072 ' ..:. .: . . '- A i r r l l i r
RaCm mean abutted R, oontrola
Bk blank control, S - sample
Tobe ample temp
x - Tobs-20*C
- v ,V
f
BEST COPY AVAILABLE
,, .5 6f 7
ft
ENV1RNOMENTAL LABORATORY
LR A'ZXs/Z 'M
OECD ACTIVATED SLUDGE RESPIRATION INHIBITION TEST #209 TEST SOLUTIONS PREPARATION
' Ipr-- ;
Test Substance: 3 . - 5 - D .iU o r e p U /o c , ( 7 3 ! <-.khV ^ o l o ^ tA/v<C---------------
Sample Description: A U r t-cU C L ~ < < ^ ( < 3 .
uU l
M ,4 C Q M \ [<:
"aft?*' -"
Analyst:
3 l 0 3 ______
Date:
Stock Solution
;/
!
; ; V ,`
t F , t
.74
'Uy
^ --7 ^
t
*
s Test Cone.
mL added
ma/L
Der 500 mL
V<*'v.< r<** ?l*ot.CeuVre\
a f 1. ,
!2.jC.'I
.'. S.
'
1G `
IO
**>1 . "<V -V*
mL M O I1) water Der 500 mL
- -*fP 4 W i-, m Lsyn. sewage , f e e d D e r 5 0 0 m L'''
<z 4 ' 2 .7 4
' 5- v ( $ 0 % * k v r-x* I ( 3 d 6 v - ^ '*
mL microbial inoculum oer 500 mL
"2 -0 0
Z GO
-W - iM I ii-J : v L if-
Total Volume mL ,, '*r`. .
SC O 5 o ;
. . s
y- .IX
ifc ^ ' *
F&y^'Vn *$"
' -
,#4#?C- ft "i*t
M W
-------------H 1
,** ` -h.*,, ** ? **:$
4f
000314
"P3 k o*f "7
. . lbJ h 3 M M % r
ENVIRONMENTAL LABORATORY
/
OECD ACTIVATED SLUDGE RESPIRATION INHIBITION TEST #209
' -.''
RAW DATA
,:v ? w:
W , v :>
" ^ .V *; -
T est Substance: 3 S --T ^ V l < r r ~ p If 'i- .A i
S .;/g ^A 'tT <L _.v>-- I lT ;
Source of Activated ^Sludge Mixed Liquor: / # / - - 1`J i j / - lO i ^ C / ' Ja <CKiP P & S l & / t f / /
Date Received: ^
Date Used: Cf / J 1/ / 3
Analyst: _ _
-? 'X-y'-jrtb- -'(f-: . "
..V : ca-.i
V
A>* '
30-M INUTE EXPOSURE
- DISSOLVED OXYGEN CONCENTRATIONS, mgrt.
iV
Test Substance
Concentration (mjJQ
1S;EK CONTROL
T'Vu ' u ^
* s v a u > -;
., TIM E-MINUT ES
Y-*% r
0v.^5s*,-r.ot'lmH 'i
2I0 .-
2.53.03.54.0
4.5
5.b`!.5.5
7.o&:<C'3C;t S.CrS3S.CM.7H.4
T
emp C
!9.S
/o
ko
,
73n.~j
*V, <J7-*j",K7.- *7,C
C.G
.^.3
. . . . \ 4'
fA*.'>'> ^*
,,y.ir ft.
riT:- '.--1v'' ii. -: .:: .< -
iv**. y*
vie,tS*- ** ^*` :.::''/i
tyf'Cv\*/ V*V->1.
'^6*'l-
IBK CONTROL U
eg
*.
2;0SSh? 1t l f?:*
,*VVM5.TmWSWm5J^-R~ -twflmySseKifi'4,-a3':/`.,vV*" CffV5% i i
,,V f i r -vA<','j *,*
[3-HOUR EXPOSURE!
____ r
:l
DISSOLVED .OXYGEN COMDENTRAT1CNS, m grt/!
v , i 1 ' " '
LCONTROLU
!rY*.a5}if5Ti.j'
b 3)Irw:Jt\i-^-|']iriKft'ibt :,S'i*
I :; r !: J*: I j !
..
iB m B w B IW ir
000315
BEST COPY AVAILABLE 000316
ENVIRONMENTAL LABORATORY
OECD ACTIVATED SLUDGE RESPIRATION INHIBITION TEST #209
CALCULATION OF RESULTS
^
Test Substance: 3.5-DICHLOROPHENOL
Analyst: GWJ
v ,
mq/L 0 .5 1.0 1.5 2.0 2 .5 3 .0 3.5 4 .0 4 .5 5 .0 5 .5 Temp. *C R i l ) w Ra (2) . V*a- 's a m m m
BK1 7 .0 6 .6 6.3 6.0 5 .6 5 .3 5 .0 4.7 4.4 4.1 3 .7 19.9 0 .6 3 6 4 0 .6 4 0 8
.. - i
- ` '1m m m &
10 8 .0 7.8 7.7 7.5 7 .2 7 .0 6.8 6.6 6.4 6.1 5 .9 \
2 0 . 3 - 0 .4 3 3 9 '' 0 :4 2 5 0 m m m ;-
-` a t. !
Mi Ml **85KP9^WSSt9
BK2
8.0 7.6 7.4 7.2 6.9 6.6 6.3 6.1 5.8 5 . 4 - * 5 :0 *
% cliff In controls (4): 10.2
0:5709$ 0.5789 E W m B R aC m I(5 )S 86 0|60 99 |
y~i
m g/L 0 .5 1.0 1.5 2.0 2 .5 3 .0 3.5 4 .0 4 .5 5 .0 5 .5 - Temp.*C^ ^ R f i l l H IR a (2V* %*inhT(3)l
jS#6 m m m . m m m m m m m
BK1 7 .7 7.4 7.2 6.9 6 .7 6.4 6.1 5 .8 5 .5 5 .3 4 .9 -1 9 . 4 ^ /-0:5552V 0 .5 7 8 8 M M M
10 8.3 8.2 8.1 8 .0 7.9 7.7 7.6 7.4 7.3 7.1 7 .0
BK2 8 .3 8.0 7.8 7.5 7.2 7 .0 6.7 6.4 6.1 5.8 5 .5 % diff in controls (4): 1.6
2 0 .4 > 0.2776V 012700 P IS C IM I
-** **
- ' A',`
-
m m m jk *
mmmm , : -.ryr;.-
19.3 0 .5 6 0 0 - 0.5879
>1,
(1) R Oxygen uptake rate (mg 02/1.-mini (2) Ra R adjusted to 20' C> (R/e.Exj (3) % Inhibition |(RaCm)-RaS/(RaCm))100 (4) % (Utterance in controls: (IRaBk1-RaBk2l/RaCml100 (Two controls must be within 15% tit each other.) (5) Mean adjusted R, controls |RaBk1RaBk2)/2
0 1.072
a
RaCm mean adjusted R. controls
Bk blank control, S sample t;sf ^ i, -
Tobt sample temp
* Tobs-20*C
"l>
, -V.-f
