Document O3ydpkwq717BR7DKNgnm3qRe
AR226-2767
FOR DU PONT USE ONLY
Copies to
E. I, du Pone de Nemours and Company
Haskell Laboratory for Toxicology and Industrial Medicine
Elkcon Road, Newark, Delaware 19711
HASKELL LABORATORY REPORT MO. 567-81
Material Tested
Octanoic acid, pentadecafluoro-, ammonium salt*
Haskell No. 12,037----
14.033
14.034
Study Initiated/Completed ------4/6/81 - 5/14/81------
.Material Submitted bs
'CR&D Department Haskell Laboratory
ORAL LD50 TEST IN RATS
Tojieteralne the effects of
if any, on the LD50 v
and
n rats.
Procedure! Thirty young adult male Crl:CD rats were. treated by single ^
intrapericoneal 1'jections with aqueous solutions ofy--------MMIB--------Jac 80 ing/kg/day for 3 days. One day^Afterthe final treatment, the 30 rats, 10
rats per group, were adoinlseeredyBBBnby incragastric intubation as a
suspension in corn oil in single doses to determine the LD50 value. The
surviving rats were weighed and observed during a 14-day recovery period and then sacrificed. The LD50 value was calculated from the mortality data using the method of D. J. Finney.**
Another group of 30 male rats was treated by singleintraperitoneal
Injection with aqueous solutions o^ul^BBIIHII^HUBBBi^ ^ mg/kg. One
hour after creetaent^U9n?es administered, usingthesame procedure as
above, to determine The LD50 value.
chefftjby An additional group of JO male rats served as controls and was
administered only
intragastric intubation to determine the LD50
value. The same procedure was used.
Company Sanitized. Does not contain TSCACW
Results:
H-12,037
Dose
(ag/kg)
I ControljBH
Average Bod y Weight (g)
Suspension
(Z)
Average Dose (mL)
Mortality
Ratio
LD50***
650
267
500
276
400
262
4
4.32
8/10
478 mg/kg
3
4.59
7/10
3
3.49
2/10
Clinical Signs;
Wet and/or stained perineal area and weight loss at all
levels tested. Stained face and weakness at 650 and 500
nig/kg. Chronodacryorrhea at 500 mg/kg. All deaths occurred within 6 days after dosing.
H-12.037
Dose
(mg/kg)
Average Body Weight (g)
Suspension
(2)
Average Dose (mL)
Mortality Ratio
LD5Q****
650
259
500
260
400
261
4
4.21
19/20tt
547 mg/kg
3
4.33
4/20
3
3.48
0/20
Clinical Signs;
Vet and stained perineal area, stained face, diarrhea and
weight loss at all levels tested. Weakness at 650 and 500
mg/kg and lethargy at 650 mg/kg. All deaths occurred within 4 days after dosing.
Dose
(mg/kg)
650 500 400
Average Body
Weight (g)
269 256 2ol
Suspension (25
4 3 3
Average Dose (mL)
4.37 4.27 3.48
Mortality Ratio
8/10 5/10 1/10
LD50T
520 mg/kj
Clinical Signs:
Stained face, wet and stained perineal area, weight loss
and weakness at all levels tested. Diarrhea at 650 and 500
og/kg. Tremors and chromodacryorrhea at 400 mg/kg and
Jacrimation at 650 ing/kg. All deaths occurred within 5 days after dosing.
-w.pany n.^-i-;ed. OOK nd --tal- TSOA CBl
Summary; There were no significant differences in the LD50 values o
either^tfheg tested alone or following pre-treatment-wlth eicherUI SollQvag^f^yyJf^SSlSSSffLs 520 ing/kg anoMBlBkpollowing
Clinical signs most frequently seen Included: wet and stained perlneal area, stained face, weakness, diarrhea and weight loss. All deaths occurred within 6 days after dosing.
H-14.033
Synonym: ||
CAS Registry No.:||
w Purity:
H-14.034
** *** ****
Finney, D. J., Probit Analysis, 3rd Ed., 1971, Caffibrldge University
Press.
95Z Confidence Limits; Lower;
Upper: Slope:
361 ing/kg 571 og/kg
7.9
952 Confidence Limits: Lower:
Upper: Slope:
517 ing/kg 582 mg/kg
22.1
-
3 -
Sanilixed-Do25 ^
Comp^
TSCACBl
contain
952 Confidence Limits: Lower; Upper; Slope:
450 ag/kg 618 ng/kg
9.8
ft
if this study was repeated. Rats were
based on all 60 rats.
Repor? by:
^f^^ ^W/ ^' i> /- John E. Henry/ Technician
Reviewed by:
^ - '
// / ./',
^^^i^/ ^7A^
0. Louis Dashiell
Study Director
}^y^^ Approved by:
.jgS^g^A^ ^f.
^r .
Gerald L. Kennedy, Jt.
Chief, Acute Investigations Section
JEH:jrg:WP:1.12 Date Issued: Sepceqber 30, 1981
iport Mo. 567-81 ^
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