Document O3rrM5ax1Lz9qg0MVXdoNByDj
AR260-0350
BIOCONCENTRATION
TEST SUBSTANCE
Identity:
CAS # 1691-09-2) N-ethylperfluorooctane sulfonamidoethanol; may also be referred to as
N1-1E1t2F,2O,S3E,3,A4l,c4o,h5o,l5,o6r,F6,M7-.374.28,28.,(8-1h-eOpcttaadenceasfullufooron-aNm-i(d2e-h,Nyd-reotxhyyelt-hyl)-,
Remarks: Material is an off-white, waxy solid of uncharacterized purity.
METHOD:
Method/guideline followed: 3M derived method outlined in the test procedure
fToyrpdee:teSrtmaitincinegxptohseuarqeuawtiitch
Bioconcentration Factorof N-EtFOSE
flow-through clearance phase.
alcohol.
GLP (Y/N): No
Year: 1977
Species: Bluegill (Lepomis nmacrochirus) and channel catfish (Ictalurus
Supplier:
punctatus)
Bluegill obtained
from
a
private
hatchery
in
Baltic,
Ohio
Channel catfish obtained from a private hatchery in Lonoke, Arkansas
AGnCalwyittihcaellemcotnrointocraipntugr:e;Ctoencmepnetrraattuiroen of N-EtFOSE alcohol in water and fish by
LengthBlaunedg`iwlewiegrhte:in the range of 2.5-3 cm and weighed 0.5-1.0 grams. ExposuCrheanpneerliocda:tfi2s1h wdeayrse (8B-l1u0egcimlla&nd14wediagyhse(dCh5-a1n0neglrcaamtsfish)
Clearance period: 14 days for both species
Statistical methods: Not noted in report. Test fish age: Not noted in report.
Loading: Not noted inreport.
Dilution water chemistry: Not included in report. Pretreatment: None
Test conditions: Dilution water: Carbon-filtered well water Stock and test solutionpreparation: "The primary stock solution was prepared by dissolving 57 grams of N-EtFOSE alcohol in 50 ml of acetone, applying the solution to 10 pounds of 3.5 mm glass beads, evaporatingoff the acetone, and circulating carbon filtered well water through the glass beads. The system was designed to result in a final loading ratioof 0.5 grams per liter. Aerobic conditions were maintained throughout and the system was assumed to go to saturation after
Ecixrpcuolsautirneg fvoers3sewlese:k3s0.-gallon glass tanks
Number of replicates: One
006217
NNuummbbeerrooff cfiosnhcepnetrrraetpiloincsat:eO: nNeotplnuosteadniengtahteivreepcoorntt.rol Water cDihsesmoilsvterdy douxryignegn:th>e5smtgud/yL:
TpeHs:t Ntoetmpneortaetdurine:th2e1re1porCt. Photoperiod: 16 hours light and 8 hoursdarkwith a 30 minute transition period.
tRheemfairshkdsidfineoldt:ePxrhiiboirttaoneyxspoysmuprteo,masnodfadtitsheeasteimoerofabtnhoerimratlriatnisefseroftobetehsatvitaonrkas,nd awepipgehatrawnicthe.a Tcohmemefirschiwalelryeafveadildaabilley batasaicradtieetof(T2etprearcMeinn.to).f their total body
RESULTS
Awnaatleyrsoifs aopfptrhoexiwmhaotleelyfi4s0h0:s1h.owTehdeflsutourdoycihnedmiiccaatledcotnhcaetntthreatNi-onEtrFatOiSosE ianlfcioshhotlo
concentration in concentrationof
t0h.e21fi3shmsgtaNb-ilEitzeFdOaSfEtearl7codhaoyls./
g
Fish having an of fish placed
intial in water
free
of
the test substance showed a decrease of 0.012 mg test substance / g of fish in
14 days (wet weight).
CONCLUSIONS
tNhoe rceolviaebrlepacgoneclinudsiicoantsescathnisberedpeorrtivweadsfrtoomdetthiesrmsitundeyt.heAletxhtoeungthofthfeluoobrjoecchteirvmeicoanl tuhpetapkreimaanrdy bpiuorcpoonsceenwtraastitoon,deivt ealpoppeaarsmeftrhoomdthfoeratbessttriancgtboinoatchceumcuolvaetripoangweitthhat tThheeisntvuedsyt'isgaotbijoencotfivbeiisacncoutmdueltaatiiloendoifnftlhueorreopcohretmibcodayl.s in fish being secondary.
Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133
DATA QUALITY
mReeltihaobdioliltoyg:y.KiiTmhiescvhigroarnokuisngm3e.thTohderueseisd atolascokluobfildiezsectrhipettieosntfsour btshteasntcuedym'as y
hsuabvsetraenscueltbeyd binotahstuepsetrosragtaunriatsemds swoalustiqouni.teTrhaepidu,ptlaeakdeiangndonceletaoraqunecsetoifotnhief test
Spoelruhtiaopns. tTesetstsusbusbtsatnacnecewapusristoyrwbaedstnootthcehafirsahctreartihzeerdt.haTnhbeeiinnitgiatlawkaetneurpqufarloitmy of
gthreeadtielruttihonanwattheersoilsubmiilsistiy.ng.ThTehreetceosvtersyubtsatnaknucseecdonacpepnetarrastitoon
used be the
in the test same for
is
both species tested.
006218
Third party review by Dr. James W. Gillett of Comell University (observations included with report) also indicate a lack of reliability.
REFERENCES
3M Technical Report "Bioconcentration of FM 3422 in Bluegill Sunfish and in Channel Catfish" M. T. Elnabarawy, Project 9970612600, Fate of Fluorochemicals, Report Number 01, May 17, 1977
3M Technical Report "Aquatic Fate ofA Fluorochemical: FM 3422." A. N. Welter, Project 9970512623, Fate of Fluorochemicals, Report Number 002, October 14, 1977
3M requested expert overview, "Bioaccumulation Studies", Dr. James Gillett, Comell University, March 8, 1993
OTHER
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Last changed: 5/18/00
006219
-3m Fomaar na
TECHNICAL REPORT SUMMARY
+4 TECHRICAL COMMUNICATIONS CENTER 20120W hmporon 1 portpinotn bah ieof asge, svt cope0 TEE)
cavironmental Laboratory (EF & FO)
5/17/77 Ginn
Fate of Fluorochemicals
9970612600
Bioconcentration of FM 3422 In Bluegill Sunfish and In Channel Catfish
01
A. N. Welter - 21-2W (58)
.
M. T. Elnabarawy - 2-3
46981
2665, pages 17-26
3
sTecaohn,nLO, c[ogoim ovaGoei Rovorcain | "RESIALD
Reign
eesostn,Sous other The purpose of this pilot study was to determine the extent of
SID a
fEluonrochembircaalni(FBMac3r4o2t2h)itunpst)akeandandC/hoarnnbeiloccoantcfeinshtra(tcitoanlubryusBlupeugnicltlatus).
FBlluootroogcyh/eAncitciavlity reing
oe (dioaccumulation)
[ET [REPORT ABSTRACT: (200250 words) This soatract informationisdhiributbeyd the Technical CommunicationsCanter10.
EE & FC - Div,
AsEumsopedeicftieaidqmueattenictchsnieqnmuvaeirraocinshmierpnertsos)pobsyeadntwdehfsoColhreasmnpnofeenialisthteocrrouirttnfhgietsnihsfsltuuhT eoo(IrsceoatcnahaFlh oulerymusisic.sae .lspiniBcnlr tuaetgiisll wTSFaotmeArooFenIvoinrpoonsSimusensnA.t,byAWactohhneiIscewv.hionFRlgieo.hrafitsoiphoe_sci(oeLsfephaonapigpsraolxnsiaomcartpoecllhayivraeu4as0u)0e:dA1w.weirtehCioannnAacsleeynvzteernda H origi fheans uptake nas noted (-0006 BEE). Bioconcentration suudics are useful to qualitatively monitor fluoro-
chemicals in water. Whole fish or tissue analysis from fish exposed to
at fluorochemicals ibni 109theiroefnvtihreosnemecnhtemimcaaylsproivneahtoabqeuataicuseefnuvlirotnomoelnti.n
J 006220
a
A'
Experimental:
A sample of FM 3422 (57 grams dissolved in 50 ml acetone) was applied to 10 pounds of 3) mm glass beads. The glass beads were then placed under the hood and the acetone was allowed to evaporate over a 48-hour period. The FM 3422 coated
beads were spread evenly in an all-glass 30-gallon tank (114 liters), forming a layer of approximately 2 cm above the undergravel filter. The test tank was
TFhiellweatetro TihssSvCeaLpIaacbiLtey Uwpiotnh rceaqvueers:t.ilbTheer.eadmuoeulnit woaFtetre:rt cChoemmpiocuanld scopmrpkoerdivwieonn of designed to give a final loading ratio of 0.5 grams per liter, For 3 weeks and
under continuous aerobic conditions (aeration was maintained at alltimes),this dynamic system generated saturated water solution of FM 3422 without organic
inter manner contatning ne Ti SoBe: solvents. Under identical test conditions, a control tank was set up in a
After the 3-week period of aerobic aging, test "ish were introduced into the
tanks (an
Fish used
assumption was made that equilibrium in the system had been
in this experiment were obtained from private hatcheries:
reached).
LeBplountesgilslacrsoucnhfiiosuhs)
(ctCahtaumreel cpmacttieotnus)
source:
satcic, onto
Lonoke, Arkansss
sie
2.53 cn
510 ca
Weight:
1501.0 grams
5-10 grams
iTuhse gFrisehsceorrethhaenld$ Banadl)c:areTdheforFisihn waedreequastcecllyisssteerda.tceod wsaateerGo(addiisisioolnvaesd voerryggeen +
TtOCheofemnpm1ee6Frr-iagshtlhouauwrlroelrylewiagashvftaodimlaaaniddbnalitela8yi-bnhaeoostduircaatdasrtraroktoreowmao(sftTeeBptmbrrpapoeevrraHiictdneuegnrd:tewio70tPF+hr2itaohnor3F.s00(-.2ms1ipe+inr1euplteesbCu)toe.r,sanwswAoimiptishithooaenstopLpteehirreeiinoodsd. 5
Gtiim%eeaocef otFhelnrortmraalnsiftoiresto.oftebsethervainekrs',ahtdhapFpLeashradnLce.not SxhIDLE mn) syapeom of
A criterion for sampling was set up. On various days of increasing periods of
exposure, representative water samples at various depths were collected and fish
wwoerree sspaacmrbiffoircreadlat10.r8andCohmo.n AatqutshreiuemndWhoifchthweaeexproosnutrientbpoenritoyd,Firrieomnaimnirng foiosohh
wSaatcreirf(iacef,lotwh-ethtrootueglh sFyrsetsehm)boitso wdeeitgehrtmiwneesthreecocrldeeadr.anceSom(e'reccohvaeprmye)d rcaattef.ishAwtore
dissected, and various parts were removed and retained for analysis.
Roses:
}
ECoxnotdnrcaGacCnttsraantoaifloyntwsiactabelyr tshaeemcrphlneeisquteoasnddinniaovqfeuesbaewceirntihfiPcearnefdoerlfmeiecsdht.roninenr-ohcoanpasrneuarlsyuzndededetrefcoctrhoerF:ldir3e4Ec2at2t,rascutpieorsns
J CCiiitloicnouonnnatoetFdh:iRsanDpredorejaeliclt:e.dcadTneiosctber.oimpFtoaiutonnderoifnchot.hrirsTe.nwterEraikvmidob1naarosrreayetehisconsesdoo:fchBndiioo5tn4e3si3oonvteedlrbeoniLnfooerma:
Nor 3660, pp. 17-36.
"006221
Ee. .
= Discussion:
Tihnrobuogthhouttesttheanedxpceornitmreonlt,tanaklsl. fisDhurianpgpepaerreidodgseneorfalelxypohsuerael,thy5 balnudegaicltlive wsausnfimsohstdileidkel(y2 dfureomtotepshtystiacnaklanidnju3ryfroamndcowanstronlottacnokn)s.ideTrheed mtoorbtaelitteyst dcuoempotuonsdamrpellaitnegd.andWeavtaeprorwaatsioand.ded periodically to supplement the loss
C7oncdeanystraotfioenxsposoufreF.M 3422 in both fish species had reached a plateau by
aMnecaesuroefdFciosnhcesnitzreatoinonbsioaocfcuFmMul3a4t2i2onin (tthhee vteisstcercaompiolulnudstrteantdesd ttohebiiom-poreconcentrate in higher ratios in larger fish).
Aafntderbeftorraensfienrtrriondguctihneg crheamnanienlingcabtlfuiesgh,ill5 spouunnfdisshoftostehae-sraencdov(ewrayshteadnka,nd
biegandist.ed)Awnearleysiosveonflywadtiesrperssamepdleosn tthaekebnotbtefoomrecovaendrinagfttehreaFdMdin3g422thceoastaendd
:
showed no change in FM 3422 concentration.
Wtheostletafnikshshaonwaeldysiinssiogfnif3ibclaunetgiulpltaskuenf(i.s0h006aftmge/rg)a. two-minute dip in the
1 you have any questions, please contact me on 3-9186.
LET LN wn
MTE/cel
5
*
Attachments
7
006222
i"
Bluegil) Sunfish (Lepomis macrochirus)
Treatment
FM 3422 Concentrations
FishR-attoi-oWater
Uptake (exposure day): In Fish (mg/p) In Mater (ppm)*
No backgromd .329+ L003 (air-off)
8
1s
12902 .005 (air-off) 407:1
1
7
L428 (air-off) 75:1
21
L213 005.580 (air-off) 67:1
Clearance (Recovery days):
o
Ls
7
L007
}
14
L012
Ette rrrr ete aerenengen 3
+ Vfaislhu.es are means and standard deviations from the analysis of 3 (whole)
-
++ sVaamlpuleessarreeprmeeasnesntianngd dsetpatnhdsardofde36v,iat2i0onasndf6rocnmthferoamn-aboltytsoins.of 3 water
y
006223
.
.
5
Treatment
Channel Catfish (Ictalurus punctatus)
FM 3422 Concentrations
Fish-to-Water
Ratio
Uptake (exposure days): In Fish (mg/g)*
In Water (ppm)**
0
No background
+340 (air-off)
7
Lzse.00 L325 (riroff) 34:1
1.075 (air-on)
119:1
1
+100
'
Clearance (Recovery days):
100
.546+.02 (air-off)
.620+.05 (air-on) =
183:1
161:1
7
L023
1
.003
yy
+ Values are means fron the analysis of 2 (vhole) fish.
** Values are means and stundard deviations from the analysis of 3 water
samples representing depths of 36, 20 and 6 cm from bottom,
>
.
"
006224
Baia
TECHNICAL REPORT SUMMARY
70 TECHNICAL COMMUNICATIONS CENTER -- 201.20N Umporant - epots rieonbdoh siodfpeapesr adtwocopes 0 TCC.J
10/14/77
Environment :
Aquat
:
wow
b.L
7
A, N,
#
swecmoomntovs,honaQn O a me EE PC - Div.
Fluorochemicals
cCoutnaeN)oueoGlTIiv moriaion
Progress Report
| "RECTED
T(BoAixqoiuccaoitnitccye)ntration) E REPORTrABSTe RACT (R00E2E50wIaErn d Toe socmrmaotaito.s Gadoy
9970612623 al
re 5
gamer
Toil Communion Corr 3
MREPI)
006225
a
INTRODUCTION
`aTsheansuibjnetcetrnceodmipaotuondin (tFhMe34s2y2n)thweassissoelfecottehderfocromtmeesrtciinagllbyasiemdpoorntanittsfliumpproorcthaenmciecals.
These compounds represent a major commitment by the Commercial Chemicals Division. With increasing governmental regulations pertaining to the influence of chemicals on the environment, consideration of the environmental impact of this chemical class was mandated. Furthermore, since FM 3422 might qualify as a 3 "critical
pcohsesmiibclael',"enveixrtoennmseinvteallabimopraactto.ry investigations were performed to assess its
Physicochemical data have been utilized to predict the behavior of chemicals
in the environment in the absence of experimental data, Available physicochemical
data for FM 3422 include the following (1):g water solubility - 0.05 ppm, partition
pFcrMooepf3ef4ri2tc2iieweson.utldinFbMen-3po4e2c2rtsawinasostle/nnwtoa,ntteorrxeislcaytswitivetemhliy-n >i1ni0tsso.lsuobTllhueebsiielniwtdayattealriwmaointusdldpwohsseusngegsesssutbmltiihtpatotepdhilic For aquatic toxicity determinations.
It is the purpose of this report to present data relative to the bioconcentration
potential, uptake and clearance rates of FM 3422 in either the bluegill (Lepomis
macrochirus) and/or the channel catfish (Iotalurus pwictatus).
MEODS
.
ME.nvTi.ronEmlennatbaalsauLyabhoarsatorreycenrtellyatdievescrtiobetdhethaeccsltiamnadtairodn mpeertihoodd,s cuatrielizoefdaqinuattihce
organisns and method of chemical exposure of these organisms (2).
Suppetcaikfeicanpdroctloecaorlasnceforrattehse duseetderimninatthiisonstouflbyiofcoolnlocwe:ntraBtliuoengilflacBtoCrFswe(rCeF),
dwoorteerneivnaeludataetdtohne 8t,he147 aanndd 2114 ddaayyss ooff deexppuorsautrieont.o FCMha3m4n2e2.l cCaltefairsahncBeCEvwaelruees
obtained at the 7th and 14th day of exposure with clearance values being
i
determined after identical periods of depuration. Bioconcentration factors
for specific organs of the channel catfish were determined after either one or
aftourthwreeeeksdifoffereexnptosulreeveltso wtihtehitnesttheflueoxrpoecrhiemmeinctaall. taWnaktoenr tsahmopsleesdawyserewheonbtafiisnhed
.
samples were obtained. The analytical techniques used for the determination of
FM 3422 levels were those routinely used by the Environmental Laboratory and will
be the subject of a report (1).
wsurs
Bioconcentration factors, uptake and clearance rates were monitored in both the
bluegill (Lepomis macrochirus) and channel catfish (Ictalurus punctatus) at
ivnavriyrionngmetnitmeasinwteelrlvalass dduurriinngg edexppuorsautrieonto (0T.a5blegs/l 1,FM2,342F2iguirnesthe1, aq2)u.atic
L
006226
;
Graphic representation of these data indicates that a rapid uptake of FM 3422
f
by both (Days 7
organisms had and 8) values
ioncdciucrarteed
t(hFaigts.a
s1,tea2)d.y
sTthaeteihnaistiableensamaptltianignepderaitodsome
mcaatrelriiearltifmreompebroitohd.orgaOnfisgmrseawtheirchimwpaosrtaqnucietewaasppatrheentrapaifdtecrlesaerveanncedayosf othfe test
ddeipdurtahteiobnl.uegiTlhles.channel catfish cleared FM 3422 somewhat more completely than
qBuiiotceoncseinmtirlaatrioanndfsaecetmorisnglayndicnldeeapreanndceentvaolfueesxpdoestuerremipneeridodfsor(wThaobllee o1r).gansThewere
weelreevatdeude tfoluaorsoicnhgemliecaslerC,ievaolfueosutloibesrervvaeldueisn. thWehecnhantnheelsecavtafliusehs weexrpeeriimgenonrtesd,
ifdleunotrioccahle,mic0a.l4
concentrations ppa.
achieved
in
both
experimental
tanks
were
gInreattheercdhaengnreele tcahtafnisthh,e rtheelatmiovreelyliploipphiidl-ifcreeormgaatnesribailcscon(cTeanbtlreat2e). FMTh3u4s2,2 ttohea
0filluorloacyehreniocbatlainbeidocofnrcoemnttrhaetisoknin faancdtortshe fvoilslcoewriang (ognute) wepeoksseosfseedxpotsheurehigthoest
eFxMpo3s4u22r.e
tBoratihne
ftilsusoureo,chwehniicchalwasshowaendalsyizmeidloanrlyelefvoaltloewdinBgCF'fso.ur
weeks of The gills
had
attained high indicative of
alevlealrsgeofsurFfMac3e422areata
baovtahilaebxlpeosufroer
bpienrdiiondgs.,
wThhiech reismapirnoibnagbloyrgans
Twhheisceh wvearleuestefsotredBC-Fmwuescrlee,appskrionx,imastkeellyetoonne-oarcdheirevoefdmasginmiitluadrelelveseslstohafnFtMha3t422.
found for the more lipophilic materials.
DISCUSSION
oInccutrherseed svtiuaditehse, giulpltsa,keoroaflFaMnd34/2o2r bcyutatnheeoubsluergoiultless. anTdracnhsainneenlt ecxaptofsiusrhe,probably
o<f2 thmiisn,fOlFuorfoischhemtiocaal.fluUoprtoackheemmiacyal-raeqsuualtticfreonmvitrheonmpeenntetrdaitdiodnemoonfsttrhaeteliuppotpahkielic
egixlplosuerpei,thetlheiumflbuyorothcihsemilciaplophcioluilcd emonlteecrulteh.e giDlulricnigrcluolnagt-itoenrman(ddaytsh)encFeM b3e422
*:
dtirsacnusspsoerdtedthetoupvtaarikoeusmecshiatensismwsithoifnnotnhieonoircgansiusrmf.actaGnrtasnmoinantdheKcooldl.bergIn (3t)heiharve
ostcucduirerse,d aitndwatshatdebmloonosdtrwaatsedthtehatprirnacpiipdalupttarkaenspoofrtthimsedicuhnemticoalthebyvatrhieougsilltsisshuaeds/
noarygabnes wdhaemraegeddebpyosietxipoonsurocecutor-retdo.xicBamsasterainadlsHeartehsul(t4)inpgositnultaitsesduethhyaptoxtheemiagiwlhlsich
ccounlcmliunsaitveedlyinddeemaotnhstorfatetdhethtaetsthoyrpgearnpilsams.ia wBiatshs eltamaellla(r5),fusiniona osufbsgielqluenftilpaampeenrt,s
and was
edema did occur duc to impaired
in the presence respiratory gas
torfanasptoorxti.cantT.heseThepapreerssultsitnregsshyptohexicimcpoonrdtiatnicoen
olfesitohnesgwihlilcshinmaytheresuupltta,kemonfiffeosrteiinggn cthheemmisceallvseswhaislea atlosxoicenreusmpeornastei.ng potential
OorfalthiusptafkleuoorfochFMemi3c4a2l2 b(yfetehdeingg)astwroouildnteasftfionradlatdriacrtect(gruot)u.te Mfoovremtehnetaobfsotrhpetion
. tFhliusorcohcehmeimciaclalbyinttohethbelooidntetostiontahlercoirrgcaunlsatoiofntwheoubloddyr;esullitveri,n gtahlel tbrlaandsdpeorr,t of
A
osmreegslaeenncittseimrv.ye, deeIttpco.hsaistiCobinreceunolfastutighoginesstmoeafdt,etrhieCahlifoluuinoertotchaehlemmio(cr6a)eltlwhioaptuolpdhtiolxtiihccenittyirsessauuselsstocoiinfattethdheewith
006227
`
oefxpotsheurechetmoiclailpooprhailicmemtaatbeorliiaclsprmoaduyctbeitnhteo rtehseulctirocfulatthieonloonfg-ttheermorsglaonwisrme.lease
Pinerctuhetanoeilouslapyeenretorfattihoensokifn.FM 3It42m2amyaybeexsppleaciunlattehde bthiaotaccFuMmu3l4a2t2iomnayofremthaiisn material clloecaarlainzceed. in this area with a subsequent leaching effect resulting in its eventual
Itnhethiensteactpiloortgansitusdmieosr, spweecidfiidcnootrgaqnuanstyistteamtse.upHtoawkeeverra,testhoefdaFtMa34d2i2d eiintdhiecratein
tlheasts
itnhanthesevienntadcatyso.rganUipstmakea
steady state (intakesoutput) had rates observed are a function of
been attained in metabolic rate,
cagoen,trowlelieghdt,whweanterreptleimcpaetriantgurteh,esefeeexdpienrgimheanbtist.s, Celtce.aranTcheese(evlairmiianbaltieosn)misrtatebse
wbeerestnaotteddtehtaetrmiFnMed342d2uriwnags ctlheearienditiianl<7daydsayso.f deOpfurianttieorne;sttwhoeurledforbee,thite cdaenteromnilnya-
twihoentheorf ocrleanortanctehersaetevsalautesthweouledarliinedrictaitmeethientperrevaslesnceinoafnaeftfwoor-tphatsoeasscyestretma.in
Tfhleuorioncihteinailcarlapimdayphbaesefolilnodwiecdatibvye aofslotwheer eplhiamsienawthiiocnhofwouflrdee beor prleosousemlpytivbeound
evidence for the release of tightly bound material.
dIettesrhnoiunleddbeinnoitnetdactthaotrgainnistmhsisrastthuedry uthpatnakeutianldizicnlgeartahnecespveacliufeisc owregraenontleychnique. 3 wIhtetwhouelrdorapmpoetarFMtha3t422thiiss allastoterrampeitdhloydclsehaorueldd bferomutitlhiezemdoreinlainpopehfifloirct otrogadnest.ermine
Ocnheemimcaayl-srpeecceupltaotre tihnatterabcitoicoonnsc.entrIantiroenc,eptpoerr steh,eormyaysbeeverdaule ttoypeasmoufltirpelciecpittoyr of artetcaecphtmoerntisn hsatvaeblebeeonr ildaebnitliefifeads.hionF,ororcxatnhpelem,atetrhiealmatmearyiable mfaoyundbinidn ttohethferee asstsatoec.iateOdf iwimtphorFtManc3e422woumlady bbee trheevedrseitbelrem.inaTthiiosn olfattwehrethheyrpootrhesniost mbaiyndianpgproximate . ptrhoelotnrgueed,sittuhiastiowno,uldinabesmuicnhdiacsatiFvMe34o2f2anwasirrraepveirdsliyblcleeabriendd.ing.If cThliesarapnhcaermaw-ere - J, cmooldoegsicoaflchteoomlicmalayupwtelalkehaavned acplpelaircaantcieonbyianquaastsiecssionrgganaipspmlsi.cable mechanisms and
In these pilot studies, we have demonstrated that Fi 3422 does:
1) aBpiporcooxnicmeantterlayteonien olridpeorphiolficmagonrigtaundseacghrieeatveirngthlaenveltshoosfe ffoluunodrocihnemical relatively less lipophilic organs.
2) Attain a steady state within 7 days.
3) Clear rapidly, channel cat>fbliuegsilhl.
tIhnethFeluaobrsoecnhceemicoafltboyxicthesigtnesstanodrgacnoinssmisderoinnegmatyheasrseulmaetivtehlayt rthaipsidmactleerairaalncewasof nontoxic under the conditions employed in the foregoing experiments. A
006228
BIBLIOGRAPHY.
(1) vendel, A. Personal communication. Report in progress.
(2) T"eBcihonciocnaclentRreapotrito,n MoafyFM17,34212977i,n MB,luTe.gilEllnSaubnafriaswhy atnodA.inN.ChaWnenletlerCeanttfiitslhe.d":
(3)
SGurranfmaoc,tanA.t
ainndCSo.d
K(oGlaldubsergM:orrhUepatakL.e),PaWtahtwearysResaenadrcEhli1m0i:n1a8t9i-o1n94o,f
a Nonionic 1976.
(4) BianssR,ainMbiocwhaeTlrouLt., aSnadlmAolangaiG.rduHeerait,h: ExCpaorsdediovIanstceurmliatrteanntdlyRestpoiCrhaltoorriyneC,hanges Water Research 11: 497-502, 1977.
(5) BEafsfse,ctMsicohfaeIlnteL.rmiCthtaernltesCTh.lorBienrrey;ExJpro.s,e aonndBAlluleagnillG. (HLeeaptohm:is mHaicsrtoocphaitrhuosl)ogiacnadl Rainbow trout (Salmo gairdneri), Water Research 11: 731-735, 1977.
(6) CPhairotui,tioCnaryCoTe.f,ficViierngtisl Ha.ndFrBeieoda,ccDuamvuliadtiW.onScohfueSdedleicntgedandOrRgaondigcerChL.emiKcoahlnse,rt: Environmental Sci. and Tech. 11: 475-478, 1977.
. LEGEND
Figure 1 - AUbpstcaiksesaindinicadtayess, exBlpuoesguirlelpDearitoad to FM 3422
CC,leavraalnuceeinidnidciactaetsescdoanycsenotfradteipounraotfiofnluorochemical in water
v
f siengdmiecnattesisabasrebintcrearoyf. intermediate values, hence the Line
:
Figure 2 - Legend as for Figure 1, Channel Catfish Data.
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006229
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TABLE 1 EFXMPO3S42U2R:E ANBDIOCCOLNECAERANNTCREATITOIMNESF:ACTOBRLSUEAGTILVLSARAYNIDNG
(CHANNEL CATFISH TM*
organisa
BIOCONCENTRATION FACTOR
DAYS
8
14
21
CLEARANCE
7
14
Bluegills >
4x10?
Channel catfish
7
4x10%
3x10?
4x10?
DAYS
1
2.102
2x02
7
71072
4x10?
1
ox107%
2 Values are rounded to nearest hundredth.
R Cy = +4 .1 ppm, Mean SD
Concentration of FM 3422 in water.
i. C, = +6 *.2 ppm, Mean SD
Concentration of FM 3422 in water.
006232
:
TABLE 2 BCAITOFCIOSNHCENOTRRGAATNISONATFVAACRTYOIRNGOFEGXPHOASNUNREEL
PERIODS OF FM 3422
organ Muscle Viscera (Gut)
Gills
Skin and Skeleton
Skin Skeleton 0i1 Layer (Skin) Brain
BIOCONCENTRATION FACTOR
1-Meek Exposure 4-Neek Exposure
3x10?
3x10?
270008
3x10'
7x10?
1.6x10
1x10%
3a0? x10?
9x102
5x10?
30
10%
specimens obtained from 2 channel catfish 5 Single analysis
Analysis of (1) channel catfish
\
006233