Document O3pnE376E2BL0RKoBw3ZY7K6p

mm cyuKiSHrrtflc: E. 1. d u P o n t d e N e m o u r s S C o m p a n y IN C O R PO R A T E D W i l m i n g t o n , D e l a w a r e 19898 POLYMER PRODUCTS DEPARTMENT EXPERIM ENTAL STATION PERSONAL AND CONFIDENTIAL c c : A. J . Dahl D. v:. K a r r h L. J . Papa Pral File I.C. - 353 - Nil 400 - 269 l&K, A u g u s t 21 , 1981 TO: DR. V. L. POKER - PPD, P a r k e r s b u r g FROM: S. STAFFORD ANALYSIS OF BLOOD SAMPLES FOR PERFLUOROOCTANOATE ( Job No. S 1 0 - 5 7 8 ; PRAL Nos. 8 1 - 3 2 9 1 - 3 3 3 2 ; N o t e b o o k L o s . E2 2 5 1 4, E2 6 2 3 8 ) As r e q u e s t e d i n your l e t t e r of 7 / 2 8 / 8 1 to L. J . P a p a , 40 of t h e blo od samples s u b m i t t e d t h e n have been a n a l y z e d f o r p e r f l u o r o o c t a n o a t e (Cg) by th e u su a l gas chromatographic method ES-567. R e s u lts and sample i d e n t i f i c a t i o n are given in the attached table. R e s u l t s f o r t h e r e m a i n i n g two sa mple s i n t h a t group ( 8 1 - 3 2 9 3 , L. S t r a h l e r , and 8 1 - 3 3 0 9 , K. P o w e l l ) w i l l b e r e p o r t e d a l o n g w i t h t h o s e f o r t h e s a m p l e s s u b m i t t e d 8/11/81. Attachment jah Key Words: Perfluorooc tanoate GC Blood Analysis There's a world ot things we re doing something about 000125 EXP000049 E ID 713863 TABLE I CONCENTRATION OF PERFLllOROOCTANOATE IN BLOOD ( a ) Sample PRAL No. 81-3291 81-3292 81-3294 81-3295 31-3296 81-3297 81-3298 81-3299 81-3300 81-3301 81-3302 31-3303 81-3304 81-3305 81-3306 81-3307 81-3308 81-3310 81-3311 81-3312 31-3313 81-3314 81-3315 81-3316 81-3317 Date Sampled 7/1/81 7/6/81 7/13/81 7/13/81 7/13/81 7/14/81 7/14/81 7/14/81 7/15/81 7/15/81 7/15/81 7/15/81 7/15/81 7/16/81 7/16/81 7/16/31 7/16/81 7/17/81 7/17/81 7/17/81 7/17/81 7/17/81 7/20/81 7/20/81 7/21/81 P.R.l 4678 4661 1702 W.S. W. S . 1913 W.S. W. S . W. S . 1759 W.S. 3726 4329 4651 3522 4652 4202 1991 3245 3663 W.S. W.S. 3787 W.S. 8862 81-3318 81-3319 81-3320 81-3321 81-3322 81-3323 7/21/81 7/21/81 7/21/81 7/22/81 7/22/81 7/22/81 W. S . 4664 3688 4105 3190 4451 GC A n a l y s i s _______________________ (b Date Analyzed [Cr ] , pg F / r b lo c 8/11 & 8/13/81 0.20 3/11/81 0.024 8/11/81 0.15 8/12/81 0.077 8/12/81 0.068 8/12/81 0.35 o o-C 8/12/81 8/12/S1 2.1 8/12/81 0.19 8/32/81 0.17 8/13/81 0.49 8/13/81 0.71 8/13/81 0.98 8/13/81 0.34 8/13/81 2.5 8/13/81 0.096 8/14 & 8/17/81 2.5 8/14/81 0.20 8/14/81 0.088 8/18/81 0.049 8/18/81 0.14 8/18/81 0 . 19 S/18/81 0.24 8/18/81 0.046 8/18/81 0.13 8/18/81 8/19/81 8/19/81 8/19/81 8/19/81 8/19 & 8/20/83 0.18 0.065 3.2 0.42 0.50 1.2 REDACTED* 000126 EXP000050 E ID 713864 TABLE I CONCENTRATION OF PERFLUOROOCTANOATE IN BLOOD ( a ) Sample PRAL No. 81-3324 81-3325 31-3326 81-3327 81-3328 Sl-3329 81-3330 81-3331 81-3332 Date Sampled 7/22/31 7/23/81 7/24/81 7/24/81 7/24/81 7/24/81 7/24/81 7/24/81 7/24/81 81-1843 4/27/81 P.R.l 4452 U.S. 3590 4673 W.S. w.s. 4647 U. S . W.S. 4678 Name GC A n a l y s i s __________________ (b) D a t e A n a l y z e d i C ] , jig F / g b l o oi 8/19/81 0.62 8/19/81 0.076 8/19/81 0.17 8/19/81 0.76 8/19/81 0.33 8/20/81 0.062 8/20/81 0.31 8/20/81 0.045 8/20/81 0.21 8/13/81 0.032 (a) A n a ly s is as d e s c r i b e d i n Lab Method ES-567 ("D e te rm in a tio n of P e r f l u o r o o c t a n o ic Acid in Blood, Gas Chromatographic Method", S. S ta f f o r d , 4 / 3 / 8 1 ) , using the p a c k e d column GC a n a l y s i s w i t h p e r f l u o r o - n - o c t a n o i c a c i d a s c a l i b r a t i o n s t a n d a r d . (b) Although the a n a ly s is i s s p e c if ic a lly fo r p erfluorooctanoate (acid or s a l t s ) , c o n c e n t r a t i o n s a r e g i v e n i n ppm f l u o r i n e f o r c o m p a r i s o n w i t h t h e r e s u l t s o f t o t a l o r g a n i c f l u o r i n e a n a l y s e s . (ppm F = 0 .6 8 8 x ppm p e r f l u o r o o c t a n o i c a c i d ) Estim a te d u n c e r t a i n t y i s + 10% r e l a t i v e sta n d a r d d e v i a t i o n . The lower l i m i t f o r q u a n t i c a c i o n i s 0 . 0 0 7 p g F / g . The d e t e c t i o n l i m i t i s "v 0 . 0 0 4 y g r / g , b u t c o n c e n tra tio n s in th a t range cannot be w e ll q u a n tita te d and are re p o rte d as < 0 . 0 0 7 . None d e t e c t e d ( n . d . ) i s r e p o r t e d f o r s a m p l e s w i t h [Cg] ,< 0 . 0 0 4 ppm, which cannot be d istin g u ish e d from reagent background. (c) Previously analyzed sample, re-analyzed for comparison with re p e a t sample 31-3291; r e s u l t of the i n i t i a l a n a ly s is (5/6781) on 81-1843 was 0.031. 000127 REDACTED* EXP000051 E ID 713865