Document O3nev7mNxK9xx1b44O2ykLQpw

TOXICITY TO AQUATIC PLANTS TEST SUBSTANCE Identity:A mixturecontainingperfluorooctanesulfonawthei,ch may alsobe referredtoas PFOS, FC-95, oras a component of FC-206CE. (1Octanesulfoniaccid)(CAS # 2795-39-3). Remarks: The 3M productionlotnumber was 502. The testsample isFC206CE (2/16/89).It'psu(itywas notsufficientclhyaracterizedt,hough current informationindicatesitisa mixtureof0.92% PFOS, 15% diethyleneglycol butylether,76.09% water,4% urea,1.28% Sultonefoamer,1.55% sodium octylsulfate1,.05% polyoxyethylenemonooctylphenylether,0.06% sodium laurylsulfatea,nd 0.05% tolytriazole. The followingsummary appliesto a mixture with incompletely characterizedconcentrations ofimpurities.Date may not accurately reflectthe toxicityof the fluorochemical component of the testsample. METHOD: Method: OECD 201 and ISO/DIS 8692 Type: Acute Static GLP: No Year study performed: 1989 Species: Selenastrumcapticomutum Source: OriginalloybtainedfromATCC, RockvilleM,D, medium at3M EnvironmentalLaboratory,St.Paul,MN. Element basis: Algalcellcounts(cells/ml). Exposure period: 96 hours StatisticaMlethods: Not given. Analyticalmonitoring: pH and temperature. maintainedinculture Test Conditions: Algal NutrientMedium: Nutrientniedium per OECD 201 and ISO/DIS 8692. This nutrientmedium providedallmineralnutrientesssentialforalgalgrowth and alsoserved as thediluentforallalgaloperations.The pH ofthis syntheticalgalmedium was 7.6adjustedto8.3. One hundred mL ofthispHadl*Vstendutrientconcentratesolutionwas added to900 mL of MilliporMeilliQ water.FinalpH 8.3. Stock and testsolutionpreparation:A 1000 nig/Lstocksolutiownas prretparedby dilutin1g000 mg oftestsubstance in900 mL of MilliporMeilliQ T water then adding 100 mL ofthe ph-adjustednutrienctoncentrate solution.FinalpH ofthisstocksolutionwas 7.9.Aliquotswere then added directltyodilutiownater intestvesselstocreatetestsolutions. Exposurevessels:250mL Erienmeyefrlaskcsontaini1n0g0mL test solutionand capped withstainlessteelclosures. Agitation: Shaken continuouslyat-100 rpm. Number of replicates:3 forblankcontrolsand 2 fortestmaterialtreatments Initiaclellloading: 1.0 X 104 cells/mL Number of concentrations: fiveplusa blankcontrol Lighting: 400 foot-candiesfrom continuouscool-whitefluorescentlighting. Water Chemistry: pH range (0-96hours): 8.3- 9.4 (controelxposure) 6.8- 7.9 (1000 mgll exposure) Test temperature (0-96hours): 24 "C (incubator) RESULTS Nominal concentrations: Bk control,100, 180, 320, 560, and 1000 mg/L Algal Growth Response at96 Hours Nominal Conc., mg/L Bk. Control 100 180 320 560 1000 Avg. Growth Rate 1.3853 1.3461 1.2933 1.0908 1.0378 0.8193 % Change inGrowth Rate ---2.8 -6.6 -21 -25 -41 Element valuesare based on nominal concentrations. Reversibilityof Growth Inhibition:Not given Remarks: Testingwas conducted on a mixtureas describedinthe Test Substance Remarks field.The valuesreportedapplytothatmixtureand not the fluorochemicalproportionalone. CONCLUSIONS FC-206CE exhibitsa 96-hourpercentchange ingrowth ratecompared tothe controlof-41 % atthe 1000 mg/L treatment. Submitter: 3M Company, EnvironmentalLaboratory,P.O. Box 33331, St. Paul,Minnesota,55133 DATA QUALITY ReliabilityK:limischranking2. Thisstudy meets the criterifaorquality testing.However, the studylacksinformatioonn purityofthetestsubstance and actualmeasurements ofthe amount offluorochemicaplroportioninthe solution. REFERENCES Test was conducted by the 3M Company, EnvironmentalLaboratory,St.Paul, MN, Lab Request number G 1358-2,1989. OTHER Last changed: 6/26/00 STANDARD ENVIRONMENTAL LABORATORY NUTRIENT MEDIUM FOR FRESHWATER ALGAE NUTRIENT CONCENTRATION IN STOCK SOLUTION FINAL CONCENTRATION IN TEST SOLUTION STOCK SOLUTION 1: MACRONUTRIENTS NH Cl mg@i 6H 2- 20 CaCl2 .2H 2 0 Mgso .7H 20 KH P6 24 STOCK SOLUTION 2: FE-EDTA 1.5 9/L 1.2 g/L 1.8 g/L 1.5 g/L 0.16 g/L FeCl 6H 0 Na 2E@;A.3H 20 STOCK SOLUTION 3: TRACE 80 mg/L 100 mg/L ELEMENTS 15 mg/L 12 mg/L 18 mg/L 15 mg/L 1.6 mg/L 80 ug/L 100 ug/L H BO MAC1'2.4H 2 0 ZnCl2 cocl 2- 6H 20 Cucl 2H 0 Na mg; 2 3H 4- 20 185 mg/L 415 mg/L 3 mg/L 1.5 mg/L 0.01 mg/L 7 mg/L STOCK SOLUTION 4: NAHCO 3 NAHCO 3 50 g/L Sterilize stock solutions 1-4 by membrane (mean pore diameter 0.2 um). 185 ug/L 415 ug/L 3 ug/L 1.5 ug/L 0.01 ug/L 7 ug/L 50 mg/L filtration SOLUTION 5: NUTRIENT CONCENTRATE (Prepare freshly before each test.) Add 100 mL of STOCK SOLUTION 1; 10 mL of STOCK SOLUTION 2; 10 mL of STOCK SOLUTION 3; and 10 mL of STOCK SOLUTION 4. . Make up to'.I 000 mL with Adjust the pH to 8.3 + O'.4L 1 SOLUTION 6: STANDARD ALGAL NUTRIENT water. 4o t Add 100 mL of toq, H-a@Id@usted Nutrient Concentrate Solution to 900 mL of water. REFERENCE , (1) OECD 984.',Alga,Growth Inhibition Test, 201. In: Guidelines for Testing of Chemicals. Organization for Economic Cooperation and Development, Paris, France. (2) ISO 1987. Water Ouality - Algal Growth Inhibition Test, ISO/DIS@.8692.-International Organization.for Standardization, ;o'@,'@Switzerland STANDARD ENVIRONMENTAL LABORATORY NUTRIENT MEDIUM FOR FRESHWATER ALGAE NUTRIENT CONCENTRATION IN STOCK SOLUTION FINAL CONCENTRATION IN TEST SOLUTION STOCK SOLUTION 1: MACRONUTRIENTS NH Cl mg8i 2 . 6H 0 2 Cacl2 :2H 20 MGSO 7H 20 KH2 P6 4 QTOCK SOLUTION 2: FE-EDTA 1.5 9/L 1.2 9/L 1.8 g/L 1.5 g/L 0.16 g/L 15 mg/L 12 mg/L 18 mg/L 15 mg/L 1.6 mg/L FeCl 6H 0 'la2 E@@A.3H 20 STOCK SOLUTION 3: TRACE 80 mg/L 100 mg/L ELEMENTS 80 ug/L 100 ug/L H BO MAC1'.4H 0 22 ZnCl 2 CoCl 2 .6H 2 0 cucl 2H 0 Na Mg; 3H 0 2 4- 2 185 mg/L 415 mg/L 3 mg/L 1.5 mg/L 0.01 mg/L 7 mg/L .STOCK SOLUTION 4: NAHCO 3 NAHCO 3 50 g/L Sterilize stock solutions, 1-4 by membrane (mean pore diameter- 0. 2-uzi) 185 ug/L 415 ug/L 3 ug/L 1.5 ug/L 0.01 ug/L 7 ug/L ..,-5m0g/L filtration SOLUTION 5: NUTRIENT CO@ Ci@4@A@E' (Prepare freshly beforeieach-test.) 100 '@L'@i@@T66K;sdLUTION@i @10 ml.-of STOCI(-SSOL6TION;@2; m L of ,STOCkKSOLUTION;-,3;@,@-' and mL, of -'ON-'4 t6i-1*0-66@t Li.@:th SOL crERENCES i OECD, I for,:wTosti"n' oi@ichi@iiiii c:o6'oeraii6n e. z ISO-; A19117.tWaEi' Qua ISO/Dl.-',8@692, rnt si - ze n 'ate- 0 is ratet taw -iz !4t -2 ii'il@IFranc ;LGiiiwi@ ibi ahLZ"tlol nt-tiu7,Laii:ni on a rr d', STANDARD ENVIRONMENTAL LABORATORY NUTRIENT MEDIUM FOR FRESHWATER ALGAE NUTRIENT CONCENTRATION IN STOCK SOLUTION FINAL CONCENTRATION IN TEST SOLUTION STOCK SOLUTION 1: MACRONUTRIENTS NH Cl 14gtl2- 6H 20 CaCl2' 2H 20 MGSO .7H 20 KH 2 P6 4 STOCK SOLUTION 2: Fe-ED.rA 1.5 g/L 1.2 g/L 1.8 g/L 1 5 g/L 0:16 g/L k.,Cl .6H 0 't-; 2E6TA.iH 20 STOCK SOLUTION 3: TRACE 80 mg/L 100 mg/L ELEMENTS H BO MRC1 .4H 0 2 ZnCl cocl 2.6H 0 2 cucl 2H 20 Na 2 mg; 3H 4- 20 STOCK SOLUTION 4: NAHCO 3 NaHC03 185 mg/L 415 mg/L 3 mg/L 1.5 mg/L 0.01 mg/L 7 mg/L 50 g/L-:@ Sterilize stock solutions 1-4 by membrane (mean pore,diameter 0.2 um). so LUTION 5: NUTRIENT CONCENTRATE (Prepare treshly.before.eacn test.) f Add..100 mL o -STOCK.'SOLUTION' 10 ml'-of STOCK SOLUTI io ML of STOCKISOLUTI ti,--2 N,-3',3; and ;'10 mL of STOCKls6LUTI N, I,-,nn w i t h iai@: Adjuit-, the@'pH" to@ 6: 3T*@ LA 15 mg/L 12 mg/L 18 mg/L 15 mg/L 1 6 mg/L 80 ug/L 100 ug/L 185 ug/L 415 ug/L 3 ug/L 1.5 ug/L 0.01 ug/L 7 ug/L 50 mg/L filtration ware AddtloO., to;7@9@OO@-m ENCES (I OECDY 98 Al -@ihibit 0 0 0 f'or"' stin-g,r@'of,@hi,eccilcalstl-oraan 'i t @-, fo CiDokpTeora-ti6n4,Ir@a,1"1n-1'd.',i-,.,DeIvlelopmen@fl..PwAMi-c@n,f@x@cpar.a ISO @1187. WistetjQuaili't g rowt filiit is I .-8642'.r*lnte -0 LO ton u elines a FRESHMTER NGAB OWM INUBrrION ALGAL CELI COWR, 0./ ldmyrowm mrnw* TEST TEST T,YPE- @IATERLA1 TESM' ALGAL SPECIES: Green A ANALYST. TREATMW vi,ItL- TOTAL OF 2S FIELDS cajwm -7 No./mL 2) @&Vj ooo WM :tSD LR Pli ZZY A"Zll 11,5too -7 000 @7010019 ;@),zSV/ 0Go 2yolooo 1/5-0/000 00 0voc)o /&0. 00 C) o 1 imi@de moqt axter; a,of e x th orms. elfoll 12 ol@!.'gAal';g'a.l-tGrow@hTjWbi.ii, e ocols: EWIRON40frAL IABORATIORY [-IMMTER ALGAE GROVM INURITION ALGAL CMI MWr, No. /ML Ha4OCfrCKMMt MEIIW TEST TEST BAX =TYPE >40;Z7- MATIRLKL TESTM, ALr3AL SPECIES: Green Algae, Selenastrum capricornutm A NALY ST: -BATE TREATMFXR TOTAL OF 2s FIELDS ODJNM zs NO./ML MEAN 1 SD Z15Liclooc) -Vao@cc 0 @5 '501 LIO LR PH Cl ,7-1 /,7 e'_ 2-0) cc, 0 Z) I'j0 f CIC-0 'iqc, ICK>o 1)1 L-401CIOC) LC4, iooov4L@ c@oo ooo, effiperi10lLtull*-"@lt@z"vq 0. 1, tpr; m e me i -U-SLZI t-protor-ois PO'en 106204-0PWO LabRequntNo r wp 9.ple Description EnvironrrwntelLab - WorkShnt ALGAL ASSAY: BOME TEST (Ak: BT) CCMPARISON OF GlGdM RATES Pop Date: MATERIAL TESTED: a Anilyst: I/ ALRAL SPECIES: Freshwater Green A12ae. @elen,strlm capricornutum TItEA7MENr CELL OOLNR 14 -DAY GRWM RATE(') 4- DAY I CHANGE G-DAY I.ilici --'Y1-'6 '1 L i..44,io-li C)9 t 01 1-7 @7-,2 y- IDIP 2 CP ICICCm, r,iti-I-L4 1.io Y,to I Ll) Sp6cific@-Gi6ith Rate' (u) n r@o-ith R2 t N 2!lln Cwtrol" @..ff Ub Requut No. /,;3 Sonple Desaiption Environmental Lab - Work Shoot pap of A= ASSAY: BOTTLE TEST (AA:BT) CCMPARISON OF GROWFH RATES Date: MATERIAL TESTED: Analyst: ALGAL SPECIES: Freshwater-Green Algae. Selenastrum capricomutum TREA7MW CELL COUNR q -DAY GR(XM RAfM%'-uL4-DAY LtLDAY TE 2, 2.Z-Zx it)@ 2-,14,@io(o-. I 4--z o@ Y-1 XI1-0I:1z:-9p,--IS3 7-2 1 -lLt xio(p I XOO!R-L VAST 2-4 I=IAAQI" I,io I)n (D) 14,1@ Z: 0 Li (1) Specific Growth Rate (u) (-?-P)oscout Chaw iu Crow:tbRate n N, ln N t rrnntral - qamplp) 100 Control