Document O3bQLg07EgokJVZ77XOmgLxzX

REPORT OF DR, FREDERICK B. IIINN OF PATCH TESTS MADE OH MATERIAL RECEIVED FRCLI SYTAUIT P11T/EARCH, IDG. The object of this investigation Yfas to determine whether or ot the various chlorinated diphenyl ccnpounds submitted or some ^r-puritics contained therein night be the causative agent producing Vr.o dermatitis which hud developed among sir,;o of the workmen in the plant. ' Large white rabbits were usad. in making the patch tosts to determine tho action of tho various materials submitted on the akin. Tho procedure was to shave the animal SC hours before tho patch was r.pnlied so that there would be no broken skin to interfere with or to aggravate the test, Tho material being tested was nixed rith ethyl alcohol, oithor getting a solution or a suspension for the purpose of diluting tho materiel. The patch v.-as left on for various 'Uriels durin tho t'sts -- some for tii. hours, others for 48 hours. The patch was then removed and the oxpoted arts a mutched for two mtets to bo. cure that tho test y.'as negative. This was done to avoid the chance of rot observing any latent or delayed action sucdi as do scsnot iices take place in making patch tests. For our cm information the matericle which vroix* negative v.'.ro applied in a concert rated iom in dap lie at o tests to cco if a dens cA it Is might net develop under these circv.artai'ces. Fight independent testa mero made with each material and wore made on different daya vita freshly prepared solutions to bo aura that- no error had crept in. Controlled tests were r.r;do each tine with tho alcohol and gauzes ucud to determine whether they might not be th,3 causative agent causing the observed lesions. Given beiew is Dr, Jlinn'o report, showing designations given the vaiioun materials, by Sworn Research fcllcvrod by Dr, Flinn'a designation and report of aacli rpoeific material: /."C2 lor lI.'CS (Lot P. r^de bo fore .'a.O.C/r Lot 3;-- All fright trots v;f\rO ne gativs. Tho n.n tests with co;.'.e;n,rrfeu mute-rial vu-re also negative, An intrador,: r.l teat was negative* . Arcelcr- 1get (Lot notebook: Ko, 170, page ILL; prepared by Harris of f4,b/o diphenyl and 4, V\t styrene and styrrru high boiler:_______ Aroelor ISCS, Lot Hotebook 170, page ILL, was fill testa including tho concentrated and intrr.domul. gat 1vo to DSW 033746 STLCOPCB4017708 Arcelor Spooled (Lot Notebook No. IPS, pago 9; prepared by Hubbard, of 75^ diphenyl und. 2b styrena and styrene high boiler). (Liquid Arocler, spociflc gravity 1.53),________ Axoelor Special Ifotabook No, 192, peg 9:-- This chemical gav a pocitiv tost with each of the eight applications. It also gave a pooltlve reaction with the intradeimal teat. The type of dermatitis was mild, Axoelor 1248 (Lot 9, made g/^o/gd): Aroclcr lad-8, Lot 9: All eight toBta gave a positive reaction. The intradermol test was alao positiya. The reaction waa mild. Arcelor 1^0 (Lot 5, made 5/2/55): , Aroclcr 124S, Lot Cj All eight tests gnvo a positive reaction with this compound. The intrndermal lust was a3.r.o positive. I feel that the dermatitis produced by this materiel was milder than the two previous compounds which were also found to be positive. In several tests it did . not make its appearance for 24 hours after the patch had been removed. Arc:lor lgft9 (Lot 3, Pry Ground; made c/ar/:5g): Aroclor 1269, Lot 5, ground dry: All eight tests wore negative. The seme is true of th? two tests medo with the concentrated material. The intradermal test gave negative reaction. Aroclor 1269 (Lot Notebook No, 176, peg 121; prepared by Harris, of 95.5/ diphenyl and d. r.tyrens and ntyrers high boiler -- dry ground) : .. Aroclcr 1239, Lot Notebook .176, pay ll, dry ground: All eight tests were negative. The concentrated tests war also negative. The intraderrcal tost gave negative roention, ... Arc'clc." 3.269 (I,oto Notebook No. 176, vega lal* .vr-o _an -ditto Iv abovs but rut- r.rcv-ju') Aroclor 1269, Lot Notebook 176, pago.lgl, wet ground: All cicht tosts uwc negative* Tko two tents made with concentrated materiel Y.'CrU ale negative. . Tha intradorraal tent gave a negative reaction. Aroclor 1269, Let 3, Notebook No, 192, j!ry; 10 (dry ground lit p At Le rui3 3/*; Aroclcr 1209, Lot 3, Notobook No 192', pago 10: The. eight patch tests uero negative. Tho tests rsda with concentrated manorial wore f.j.so negative, Tho irtradermal tests gave a negative reaction. DSW 033747 STLCOPCB4017709 Halowax yflOOO: - . nalcrrna 1000: Tills compound gave a poaitiva test in all of the eight testa in T.hich it vraa applied. The inaction vao different from thut of the three Aroclora giving a positive reaction. They Trere mild, but this material gave an uloerativa area. Halo>,ay ylOOl: . . HoIov.ct 1001: All tests rrith this compound, loth concentrated and diluto, irore negative. Ualecax ^1004: ' . : . Halovrax 1004: All tests rrlth this material, both concentrated and dilute, rez-e negative. Ptvrciio ri ch lor ido (17otebook No. l?p., pr.go 1C-): Styz'eno Bichloride; notebook 1V2, pago 1G: Kvcry test made with this material wea positive. It was noticed that the rabbit pulled avay v.liou this chemical vao epplied even in the dilute farm. Thio also gave an ulcerative lesion. Arocloy l'AA-3 Special (Patch 0, Sannlo 04 ~ eda lo/d/kO): Aroclor lh-iC Special Butcii D-CWi. Made lO/d/sO; This Aroc.lor gave a very slight reaction vlth tho skin but one must say that it la positive. Aroclor 1SC0 (Lot 02, made c/ps/sl): Arcelor ISGO, Lot Made o/:iZ/'61): All ci flit testa were negative, ho intradc-nr.nl or c one ont rated toots it ere- made,. Areloz* lrjC-S (hatch L'A, Scsiulo G, Mato 7./o/sO) : Aroclor iLfchv Fateh FA. Hade v/f'/sO: All tests v-oro negative). Ci . | n-.- rt:hv; .0 (- Its 1 ) Cblcr ethyl. l-easons# hot ebook 1?S, pore Si: 1'very test mado with thin material gave a positive test, Tiie locion was of on ulcerative type. Ghlt-rinotpd St;-tono fhotobook ;:o. 19?., --ro IP -- r./l C./g.-t) : Ohloz'j i-f.toci Styroro. Notebook 1*1 s, pago Ifl. s/lc/34: livery test rondo v.ith this coripour.d gave an u5ccrativ> lesion. DSW 0337^8 STLCOPCB4017710 I 4 V i *\ /\ \ i \ Dr* Flinn's comments era copied verbat in, ns follows: "C etna cut as One is impressed with the fact that eech of tho Aroclora giving positive reaction wore of a fluid nuturo, Attempts were mad to expose th animals to vigors but observations made us conclude that tho only difference wan that the eninal would be exposed to the hot material cod it was well lerowa that th reaction where such an exposure io given i3 more never. On cannot but feel that any ctyreno compound which may be found to bo present as an impurity is tho cause of your trouble* I was rather surprised that more of the compounds submitted did not show or five reactions with the skin, It has been shown in some investigations that chlorine did not produce a dermatitis vhen netallic electrodes were used but did if t)s motellic electrodes were replaced by carbon electrodes, Th theory was advanced that uomo organic chlorine compour.da wore produced in tbs latter case. I would suggest that you study your ventilation system in places where funs' are givjn off. That neana he provided for tho non to take a bath with scop and water if they- costs in contact with the type of notorial found to be positive* By this I mean if a leak or spillage occurs. The irrr.-disto bathing under these circumstances should b insisted on, I found that where 1 had gotten tho material on my Lands if I washd thorn thoroughly no trouble arose. 1- ' T t (Signed) Irodoriel: 13, Hinn l DSW 033749 STLCOPCB4017711