Document O3NRXK4M7JL4oedxdaqO2KrQw

AR *5 6 -o s y ^ ACTIVATED SLUDGE RESPIRATION INHIBITION TEST TEST SUBSTANCE___________________________ Identity: A mixture containing perfiuorooctanesulfonate, which may also be referred to as PFOS, FC-95, or as a component of FC-206CE. (1Octanesulfonic acid) (CAS # 2795-39-3). Rem arks: The 3M production lot number was 502. The test sample is FC206CE (2/16/89). Current information indicates it is a mixture of 0.92% PFOS, 15% diethylene glycol butyl ether, 76.09% water, 4% urea, 1.28% Sultone foamer, 1.55% sodium octyl sulfate, 1.05% polyoxyethylene monooctylphenyl ether, 0.06% sodium lauryl sulfate, and 0.05% tolytriazole. The following summary applies to a mixture with incompletely characterized concentrations o f impurities. Data may not accurately reflect the toxicity o f the fluomchemical component o f the test sample. M E T H O D _________________________________________________________ Method: OECD 209 T est type: Acute static GLP: No Year Com pleted: 1990 A nalytical m onitoring: Dissolved oxygen concentrations, pH and temperature. S tatistical m ethods: Results were determined by calculation of the % inhibition in respiration rate. T est organism source: Activated sludge mixed liquor collected from the Metro Waste Treatment Plant, St. Paul, MN Test condition: Dilution w ater: Millipore Milli-QTM water Synthetic sew age: per OECD method Reference and te st solution preparation: A stock solution of the reference substance, 3,5-dichlorophenol, was prepared by dissolving 500 mg in 10mL 1N NaOH, diluted to 30mL with Millipore Milli-QTM water, then brought to the point of incipient precipitation with 1N H2S 0 4 and diluted to 1L with Milli-QTM water. The pH of the reference solution was measured to be 7.2. Test substance was added directly to the test vessels and was apparently miscible with water. Test vessels: Not given. Number o f concentrations: three plus 1 reference substance and 2 blank controls Tem perature: 1 7 - 1 9 C Total suspended solids and pH on day o f testing: 7.853 g/L (TSS), initial pH 6.7 and final pH 6.9. Initial TSS in 1000 mg/L concentration was 3.14 g/L, initial pH 6.7, final pH 6.8, and MLSS 3.34 g/L. 001764 Elem ent Basis: Respiration inhibition as determined by oxygen consumption. Rem arks: Values were corrected to 20 C for calculations. RESULTS___________________________________________________ Nom inal concentrations: 2 Blank controls, reference substance, 320, 560 and 1000m g/L Elem ent values: 30-minute EC* = >1000 mg/L 3-hour EC50 = >1000 mg/L Element values based on nominal concentrations. Rem arks: Testing was conducted on a mixture as described in the Test Substance Remarks field. The values reported apply to that mixture and not the fluorochemical proportion alone. C O N C L U S IO N S ___________________________________________________ The FC-206CE 3-hour EC50 for activated sludge respiration inhibition was determined to be >1000 mg/L. Subm itter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY________________________________________ ___________ R eliability: Klimisch ranking 2. Testing meets all criteria for quality testing. However, sample purity was not properly characterized and the study lacks analytical confirmation of the amount of fluorochemical proportion in the solution. R E F E R E N C E S ____________________________________________________ This study was conducted by the 3M Company, Environmental Laboratory, St Paul, MN, Lab Request number H 1 4 8 0 -2 ,1990. O T H E R ___________________________________________________________ Last changed: 6/26/00 001765 ENVIRONMENTAL LABORATORY ACTIVATED SLUDGE MIXED LIQUOR LU H IH B O L Dat Analyst Type Test Acfivaiad S l u r i ^ ^ s p i r ^ J m l^h\bv4-\nin"Tg^ QEATVttrgrft Source ________________ Date Obtained ,, . . . . _____________ Date Uaed_____ 5 / 2 -5 / q Q Mixed Liquor Suspended Solids (MLSS) g/L Adjustment _____ g/L PH-ittIiiaLu,in>.-final kfl _________ - , Comments ' .~ Test Substance Sample Description P C llnQ E ^ ccMcevdar^o^-tfisAa&jiooorn^lu ______ ;_________________ Sample pH ^ a j lo.fi_____ Adjustment________________________ _ Comments M L rtf iia^/u^aifKMr) Att^fL Reference Substance Sample D e a c r i p t l o n ^ ^ ^ c h l m i l g n Q l ___ _____ Ki^keart cowccKtraiJon tcaRjed,'Orr^/u SamP le PHJnHal.lf,S..?f i m L fct ________ Adjustment Comments 5 SWacU acAoUe^i f U M . L ___________ _ Exposure Conditions Test Duration,^) Test Temperature 1*1 -- iq Comment 8__________________ 001766 Sample Description Environmental Lab -- Work Sheet OECD ACTIVATED SLUDGE RESPIRATION INHIBITION TEST #209 Lab Request No. H lh tfiQ d L P a g e ______ of Date: 2 jA j9 n Analyst: Test. Solution; Preparation ETC.. Z\q CF--- bcrL ,SQ2^ ( z f \ t^/PW) l^ s f . : -- \e .( \r y .U n c j ^ a m t j U c y d j m L s e .ib y ^ -w V H 'i w ia b ar;__________ k id W id u ^ XAi^tqVths u ^ g r i__________________________________________________ T e s t Cone, rag added ml M3 w ater ml sy n . sewage m m M ik -----J t f e M IBl----JBg i8W ll~ ------^ ^ r S f l O a l ml m icrobial T o ta l volume inornlnm nor Sflfl ml ml - $ m ______ I b O _____ 4- l b ' -*____________ lio Q D _____.___ =__ 5 Q Q - - - 5 b 0 ______ l & __ __ 2 2 $ \(q______ ____ LQO_________E__ g f t O . -1QQQ* _____5 0 0 _____ Z & 'L l b t ____ 2j QjD_____ r _____ a s & n b o l l ___ O___ i:___ 2 -^ 4 \U , r ____ D O .._______ 3. _ g O O___ S h a re B K fW rt-rn \ IT v m fiO -4 *S\igW K Avmou^vt- r> -0a*Tr\ -Eorvngrl.___________________________________ -- rrV ia ^ A s ijjrW ^ "H S S ,. ENVIRONMENTAL LABORATORY OECD ACTIVATED SLUDGE RESPIRATION INHIBITION TEST RAW DATA #209 LR M u B 2 L Test S ubstance:FC l icrC .fi . Source o f A c tiv a te d Sludge Mixed L iq u o r :M efytt Date R e c e iv e d :, K j l h j z j f t ________ Date Used: A n a lyst a / f * / g ? n _________ }Mi 30 - MINUTE EXPOSURE le s t Substance C o n ce n tra tio n (mg/L) CONTROL I K in 0 in r tfi 0.5 1 ,4 ln ,0 AO \Ao 1 . 0 1 .5 2 .0 2.5 la K A f t A 3 4 ,1 U . ln 4 , 0 A 4 , ? , R TIM- MINUTES 3.0 3.5 4 .0 3 ,1 A ,\ n 1 ,3 4.5 Z ,,b n ,R 5 .0 .5 .5 i l i j L \.lo i . x 1.1 If li 3E$1 CO 3 V nA |\ /A 'M ll , ILA B L fc CONTROL I I AS. ii . 4 r O -2-S L iS - I i4 B -iy 1 ,4 3 _ ii 3 - HOUR EXPOSURE le s t substance C o n ce n tra tio n (mg/L) CONTROL I *nh m oo DISSOLVED OXYGEN CONCENTRATIONS mo/L Tim e - mINUTES Te 0 .5 1 .0 1 .5 2.0 2 .5 3.0 3 .5 4 .0 4 .5 5 .0 .5 .5 b .fS (0 . ( A l o 4 . 4 +,'4 A R 3 Z l ? A ? ,, 0 I . 5 1.0 i f in.K 3 , 1 , 3 ? , 4 ./ 4 ,1 ,A.fS , 4 1 .4 I x2 l - 0 & - i f 3 .4 , 3 .3 4 ,1 4 ,? r A ir> A I 2,,F i 2 , 0 1 ,4 \ , iS 3 , 1 ,5 .0 4 .4 ,3 ,1 3 ,1 ? i,S 1,4 v .4 C l.fi l< CONTROL I I A . i2 _ pH o f A c tiv a te d S ludge M ixed L iq u o r : pH o f h ig h e s t c o n c e n tr a tio n t e s t e d : Test Tem perature: 1*1- IS Q - . x a . A A .A X x i. IZ l In itia l I n i t i a l Ip.'l F in a l F inal (p,6 If 001768 fcNV IKUNMbNTAL LABORATORY ACTIVATED SLUDGE RESPIRATION INHIBITION TEST #209 CALCULATION OF RESULTS T e st Slihgtflnrp P J! IXXo-CS., {LfUof&K) 30 - MINUTE EXPOSURE T est Substance C oncentration (mg/L) Oxygen Uptake Rate R L_I__________________ 1 _______^ 7 0 l.n flfio 1000 I* '2 7 4 ^ * R C orrected^ to 20 C 1, l 0 ^ L R J lL m ^ 4 . Date JS / & . Analyst %In h ib itio n in (2\ R e s ir a tio n R a te 1*' i'O 'T ) CONTROL I I _______ __________________ ______ i . a s g i _______ P e r ce n t D iffe r e n c e in Cont: r o ls (3) ECS0 (95% C onfidence Limit ma/T. T est Substance C oncentration (mg/L) IL I ____ _ ^2 0 Innft Oxygen Uptake Rate R I.IW l.OStol l.rtlST \.iieqq R Corrected to 20* C 1. i s m (. 18ZR %Inhibition in R espiration R a te ^ 2 ,rl ( 09 ^ \ * CONTROL I I (.(m i P e r c e n t D iffe r e n c e in C on trols,C3) ECsq (95% C onfidence L im its) mg/L. A M 9/* * (1) Temp. C o r r e c tio n s L,fib.s. (mg 02/L-min.) ,,lilS&ia____ ;____ (3) %D ifference I R C . " RCJ f X IOD fa ^ K ; rc2>\ (2) I I n h ib itio n 5^ - 7-------x loo f a * cj Where: R Oxygen uptake r a te (mg 0 2/L -m in .) T ,ob s Sample tem p.,*C ft - 1.047, x T,obs - 20*C C Control The two c o n tr o l r e s p ir a tio n -r a te s sh ou ld b e w ith in 15% o f each o th e r 001769 not-calculate., o*vjge*i d eleted -too )w. tinawL one. minute. F o r n iv a n o - * D; PWO Sample Description _ 5 , 5-ch\0rX^Vlcifl0l t ^ i*0 K tf-fore y \c c MWiauhce v*i ' a- U o t D I iOUO-0 Environmental Lab -- Work Sheet OECD ACTIVATED SLUDGE RESPIRATION INHIBITION TEST #209 Lab Request No. JJ_liiSQ_ I- P a g e ___ \ of 5 Date: Analyst: Stock and T est S olu tion s Preparation - .5 ).5 r ^ v c k ilo r o p H ^ l__C '^ ^ f& rv y tc e , ^ u h a fc a v ic g , Q o r SOLUTION: SQQjrng 5 3S-J)vch\rt>pVlgvio\ (>C)/.L>____k ricH >vnli = _____________ i l t e l y ed-fSQmg .\ n - , l m l j 1.0N NaW____________ _____________ -TTVtVjfcgd -be* \Afi-Bri ____________________ _ _____________ -A dded LOSTUj^SQn dynpwuse uvAil vrip>e^..pepr\pi^df^ _____________ ~rPilvitr.d..~hQ-ovig* litx y w l i fcdillirQv^ahfy__________________ T e s t Cone. ml s to c k s o ln mlMQ w ater ml sy n . sewage ml m icro b ia l Total volune mg/L p er 500 ml p er 500 ml fe e d p e r 500 ml inoculum p er 500 ml ml I Q .. 1 0 _________+ -T 4-_____ ______ 11__________ ______ZjQO_________ _r___ 5 0 Q . 5 to & r* > c [ ~]PsV^ fhv^-yriL^ \ki'A \\_ IC K ^ 2 ^ 4 ,5 . OOi r fV Q ENVIRONMENTAL LABORATORY OECD ACTIVATED SLUDGE RESPIRATION INHIBITIO N TEST RAW DATA #209 LR_ l4 m n Zo* $ Test Substance: H ln m p V ]r.n o l A k b s k a v ip p . l ___________________ _ Source o f A c tiv a te d S ludge M ixed L io u o r : M tfjfano Va / 'TT^sh^nrrrL'PlRytL^ S L T fe u l} M y* Date R e c e i v e d __________ Date Used; 3 / z ^ / R f t ___________ A n a lyst: 30 - MINUTE EXPOSURE test substance Concentration (mg/L) 0 .5 1 . 0 CONTROL I 1 ,4 in 1 ,5 DISSOLVED OXYGEN CONCENTRATIONS m o/L i m T O utes 1.5 2.0 2.5 3.0 3.5 4 .0 4.5 5.0 5.5 5 ,ft 5 ,5 4 ,1 l o . l o \(> & ' S .S *4,2 r 5 , 1 5 4 5 ,0 ,5 ,1 Z j>Io U \.I/J 4 ,ta 4-.Z, 5 , f t 3 . 4 ic A \ CONTROL I I J. A S u 1 & . s\ z y \ f l \,4 >& . 1 3 - HOUR EXPOSURE DISSOLVED OXYGEN CONCENTRATIONS mo/L le s t Substance C oncentration (mg/L) 0.5 1 .0 1 .5 2.0 TIME-M INUTES 2.5 3 .0 ' 3.5 4 .0 4 .5 5.0 .5 .5 1o CONTROL I ( o 5 (rt.l 5 .1 4 4 4 , 4 5 4 5 i? r 2 ,, 4 t , 0 1 ,5 1 ,0 I tr> 1 , 1 1 ,in 1 , 5 1 , 0 ifl.5 ln .0 5 , 4 5 , 5 5 ,2 . 1 BEi H O n p y / l/A l 1 % ti -- CONTROL I I _ L 3 _ 4 4 A f i. A 2 u L A h L J A - 1 L . A S . pH o f A c tiv a te d S ludge M ixed L iq u o r : pH o f h ig h e s t c o n c e n tr a tio n t e s t e d : Test Tem perature: I n i t i a l (.`I In itia l F inal ( 4 F inal *1 0 001771 Test SnWanrA ENVIRONMENTAL LABORATORY ACTIVATED SLUDGE RESPIRATION INHIBITION TEST #209 CALCULATION OF RESULTS (j^f^rv~rv\Cfi<^Aih^=PMCp) 30 - MINUTE EXPOSURE T est Substance C on cen tration (mg/L) _____ 1 0 SE r c Oxygen Uptake Rate R _______ L rrtJo^__________ R C orrected^ t o 20* C l.lflCO.tfl02. L R .H 1,43 0 Date Analyst' % I n h ib itio n i n f2 R e sp ir a tio n R a te 1* Z,8 ... 1 .......A A Z S n ____________ ______ ................................ Percent D ifference in C o n tr o ls^ ECgg (95% C onfidence L im its) m g/L________________ T est Substance C oncentration (mg/L) CONTROL I ID Oxygen Uptake Rate R 1. i Q f l O.^2>0 R C orrected ^to 20 C l. S fili %Inhibition in R espiration R a te ^ * CONTROL I I i . n n i s i __________ P ercen t D iffe r e n c e in C on trols (3) E C y ^ 9 5 l^ o n f id e n c e L im its) mg/L. (1 ) Temp. C orrections. ---- ( mg 0 2/L -m in .) f c l ; RC2 \ (2 ) I I n h ib itio n ' / 1 ' v ^ * X 100 f a RCj Where: R - Oxygen uptake rate (mg 02/L-min.) T,obs Sample temp./C c & - 1.047, x - T,obs - 20*C C Control > 1 Sftl/I (3) I D iffe r e n c e s --r ---------- #*X 100 (RC, + VC\ r^ 7 The two c o n tro l r e s p ir a tio n r a te s sh ou ld b e w ith in 15% o f each o th e i 001772 .w i n | i w | I w v | J I w g ] 3M Environmental Laboratory - Raw Data Sheet (inlTttnhaawd)pttaUt---rlSattt--Tn,. sSec. c5sMho03jd. _ n K. MethodReference:Std. Methods(14thed.)pfr M08,sec. 3M Ntetsro W a s te T re k m & & 'V \a n t , C^b.^auJi ,Mv\. Calculation: mg/l Residue = mgresidue x iooo ml sample M fte o U uov~ (skfca\v<Aa- used a t o f a q ) K o r U P; H l ^ e O - Z . j M j S cp 5 ,5 -D Q P DeSsacmripptleion WMweHW&ol$Duadste. . tt.V&ui jMa- ' 2. ReportedResult mg/l ** *C20U, 4 _________ d lo 8 >UriwPCZUB q ir> _________ 11 JStS^.a_S_ft_^e-J2. tA>olt4&ol', _____ lit _________ ml Sample 8 5 5 8 A a fi ft fS __ A .8 < fi .8 GrossWeight after 105 #c 1. 1.848 AA&&\ 1. 1>48 1.141 f i 1. t t L f i U3bS I.L5 S2, 1.15314 \.ls 4 l U A I? \,13?>4 1, i a ^<: 1. I8 Z 2 . .I.l*>s8 i.iago V iS Ito ' Weight (g) l.llSlo I.IW1 I.IJLS2, UZ08 UZjCR \.\zn* I.IM4 1.1178 1.1184 k 1111 1. 1m 1. llftlo 1.1184 1. 118*1 I.IU Residue Weight <g) 0 .088Zj A .0383 A.n l8S O -D tS T A . m .83 n . is s o.idq ri.m -s '-f 0 . A 1lo^f .U/j^T n.r> ik>9 A . Al /oft D.01// n ./in o Residuemg/l Comments *1840 Aior> 3?>\4 A 1 A A 1lz>0 X \fiO 3 .\ 4 n .^ 0 0 AMA "N > ^ 7 8 5 3 -^ 1 2 .^ 5 ^ X -5 \ 4 0 HOwq/L 3 "A !4 'TC?5-/ i 12 X =5U<r0lL^)vWU y ^-5 3 4 0 -='-*--4- r0vwiQtoilw(. AM A . * 4nn ^ 8( - 35T5D -20vmJ l> y BES CO PY AVA ILABLE 00177}