Document O3BKrewExMnnKnbq46qrJ4yDQ

t4 R & H 6 - 077/ ACUTE TOXICITY TO AQUATIC INVERTEBRATES (DAPHNIA MAGNA) TEST SUBSTANCE________________________________________________ Identity: Mixture containing perfluorooctanesulfonate which may also be referred to as PFOS, FC-95, or as a component of FC-203FC. (1Octanesulfonic acid) (CAS # 2795-39-3). Remarks: The 3M production lot number was not noted. The test sample is FC-203FC, identified by the laboratory as R1335. Current information indicates it is a mixture of 0.85% PFOS, 17% diethylene glycol butyl ether, 74.46% water, 2.34% acrylic foamer, 1.7% alkyl ether sulfate, 2.55% sodium octyl sulfate, 0.12% sodium nitrite, 0.09% sodium molybdate, 0.85% triethanolamine, and 0.04% tolyltriazole. The following summary applies to a mixture with incompletely characterized concentrations of impurities. Data may not accurately reflect toxicity of the fluorochemical component o f the test sample. METHOD:_________________________________________________________ Method: OECD Guideline 202 Type: Acute static GLP: No Year completed: 1997 Species: Daphnia magna Supplier: In-house cultures, maintained at AScl-DETD, Duluth, MN. Analytical monitoring: Temperature, pH, conductivity, and dissolved oxygen. Exposure period: 48-hours Test organism age: <24 hours old Statistical methods: EC5ovalues calculated using the Trimmed Spearman- Karber method. The NOEC was calculated using Steel's Many-One Rank test using Toxstat statistical software, Version 3.1. Test conditions: Dilution water: Dechlorinated City of Duluth, MN tap water, post carbon filtered, aerated for 24 hours prior to use. Dilution water chemistry: Hardness: 44 mg/L as CaC03 Alkalinity: 42 mg/L as CaC03 Conductivity: 145 pmhos/cm pH: 8.05 Lighting: Cool-white fluorescent lights. A daily photoperiod of 16 hours light and 8 hours dark was maintained throughout the testing period. 001358 Stock and test solution preparation: A 1000 mg/L primary stock solution was prepared by dissolving 2.5 g test solution in 2.5 L dilution water. Exposure concentrations were prepared by serial dilutions. Exposure vessels: 250 mL beakers with 200 ml_ exposure solution. Number of replicates: 4 Number of daphnids per replicate: 5 Number of concentrations: five plus a blank control Water chemistry during the study: pH range (0-48 hours): 7.93 - 8.05 (control exposure) 7.87 - 7.92 (1000 mg/L exposure) Temperature range (0-48 hours): 20.4 - 20.5 C Conductivity range (0-48 hours): 145 - 161 pmhos/cm (control exposure) 1 5 9 - 1 5 9 pmhos/cm (1000 mg/L exposure) Dissolved oxygen range (0-48 hours): 8.6 - 8.9 mg/L (control exposure) 8.7 - 9.0 mg/L (1000 mg/L exposure) RESULTS____________________________________________________ Nominal concentrations: Blank control, 130, 216, 360, 600, and 1000 mg/L. Element values: 48-hour EC5o = 653 (396-1077) mg/L 48-hour NOEC = 360 mg/L Element values based on nominal concentrations. Remarks: Testing was conducted on the mixture as described in the Test Substance Remarks field. The values reported apply to that mixture and not the fluorochemical proportion alone. C O N C L U S IO N S ___________________________________________________ The FC-203FC 48-hour EC50 was determined to be 653 mg/L with a 95% confidence interval of 396 to 1077 mg/L. The test substance 48-hour No Observed Effect Concentration (NOEC) was 360 mg/L. Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY______________________________________________________ Reliability: Klimisch ranking = 2. This study meets the criteria for quality testing. However, sample purity was not properly characterized and the study lacks analytical confirmation of the amount of fluorochemical proportion in solution. 001359 REFERENCES This test was conducted by AScI Corporation, Duluth Environmental Testing Division, Duluth, MN at the request of the 3M Company, Lab Request number R1335, 1997. OTHER_____ ______________________________________________________ Last changed: 6/26/00. 001360 AScI Corporation/AScI-Duluth Environmental Testing Division AScI Study ID# 5010-068 STUDY-TITLE ACUTE TOXICITY OF R1335 TO DAPHNIA MAGNA DATA. STANDARD OECD GUIDELINE 202 STUDY COMPLETED April 1, 1997 TESTING FACILITY AScI Corporation/AScI-Duluth Environmental Testing Division 4444 Airpark Boulevard Duluth, MN 55811 Tel. No. (218) 722-4040 Fax No. (218) 722-2592 STUDY IDENTIFICATION NUMBERS AScI Study ID# 5010-068 3M Company Lab Request# R1335 Sponsor: 3M Company Sponsor Study ID# R1335 1 001361 AScI Corporation/AScI-Duluth Environmental Testing Division AScI Study ID# 5010-068 1.0 GENERAL INFORMATION Study Title Data Standard Sponsor Sponsor's Representative Sponsor's Lab Request# Testing Facility Principal Investigator Project Director Testing Facility Director Definitive Test Dates Data Validation and Report Review Retention of Raw Data and Final Report Location of Raw Data and Final Report Acute Toxicity of R1335 to Daphma magna. || OECD Guideline 202. | 3M Environmental Technology and Safety Services, Building 2-3E-09, 935 Bush Avenue, St. Paul, MN 55133. Susan A. Beach, 3M Environmental Technology and Safety Services, Building 2-3E-09, 935 Bush Avenue, St. Paul, MN : 55133. Tel No. (612) 778-7452. R1335. AScI Corporation/AScI-Duluth Environmental Testing Division, 4444 Airpark Boulevard, Duluth, MN 55811; Tel. No. (218) 722-4040. Tina Galloway. a Joe Amato. Donald Mount. March 25-27, 1997. 7&JC Two years from the date study is completed. AScI Corporation/AScI-Duluth Environmental Testing Division, 4444 Airpark Boulevard, Duluth, MN 55811; Tel. No. (218) 722-4540. 14 Sponsor. 3M Company Sponsor Study ID# R1335 2 001362 ;'ay>- AScI Corporation/AScl-Duluth Environmental Testing Division AScI Study ID# 501-068 2.0 OBJECTIVES) fTo determine the 48-h EC50 and 48-h NOEC (no observed effect concentration) of the test substance, R1335, for Daphnia magna. This study was conducted according to the AScI study plan retained by the Sponsor and OECD Guideline 202 (OECD 1993). Test Substance Test Organisms Dilution Water Exposure Chambers 3.0 TEST METHODS - /iV a Properties: R1335, amber liquid, specific gravity 1.0 (water = 1), stable, and miscible with water. Test concentrations: 0 (dilution water control), 130, 216, 360, 600, and 1000 mg/L. High test concentration solution prepared by diluting 2.5 g o f test substance in 2.5 L of dilution water. Lesser concentrations were prepared by direct addition of high concentration into dilution water. A 0.6 dilution series was utilized. Source: Healthy, less than 24-h old Daphnia magna neonates obtained from AScI stock cultures maintained in water of test dilution water quality. Use in test: Gravid adults isolated in dilution water 24 h before the test initiation. Used 5 organisms/replicate and 4 replicates/test concentration. Handled with a smooth bore pipet with an inside diameter of 6 mm. Did not feed during the test. Source: Dechlorinated City of Duluth, MN, tap water. The most recent chemical analysis is in Raw Data Package. Characteristics: At test time, the water had a hardness of 44 mg/L (as CaC03) and a pH of 8.05. Aerated for 24 h prior to using in the test, j Type: 250-ml borosilicate glass beakers. ,. Use in test: 200 ml of dilution water or test solution/chamber. During the. test, kept covered, except when experimental observations were made. ------ Sponsor: 3M Company Sponsor Study ID# R133S 3 001363 AScI Corporation/AScI-Dululh Environmental Testing Division AScI Study ID# 5010-068 ] Incubation I Observations Endpoint Calculations Duration: 48 h. Daily photoperiod: 16 h light and 8 h dark, maintained using cool-white fluorescent bulbs. Temperature: 21 C. Biological: Daily- gently agitated the exposure chambers and then counted immobilized and dead test organisms (immobilized organisms were those that did not swim within 15 seconds after exposure chambers were gently agitated). Did not remove any daphnids from the exposure chambers at 24 h. -u D Water chemistry: (1) At test initiation- total hardness and alkalinity, (2) daily- temperature, pH, DO, and conductivity. All determinations were made according to APHA methods (1995). EC50: Trimmed Spearman-Karber method. NOEC: Steel's Many-One Rank Test (TOXSTAT statistical software, Version 3.1, University of Wyoming). ii Sponsor 3M Company Sponsor Study ID# R1335 4 001364 AScI Corporation/AScl-Duluth Environmental Testing Division AScI Study ID# 5010-068 3.0 RESULTS I Test Substance Toxicity (Pertinent data are in Table 1) Ranges of Water Chemistry Parameters 24-h ECS0: > 1000 mg/L (not calculable). 48-h ECS0: 653 mg/L (396-1077)., 48-hNOEC: 360 mg/L. Total hardness (as CaC03 mg/L): 44-46. Alkalinity (as CaC03 mg/L): 42-46. Temperature (C): 20.4-20.5C. DO (mg/L): 8.6-9.0. j.i pH: 7.76-8.05. Specific conductivity (umhos/cm): 142-161. 4.0 TEST QA/QC QA/QC Criteria Test organism dead, immobilized or trapped in control exposure must not be more than 10%. The lowest DO concentration at test termination must be at least 60% of the air | saturation value at test temperature. | Test duration must be 48 h. | Reference substance toxicity test (this test is | optional). Data 0% dead, immobilized or trapped. T The lowest DO concentration measured was 97% of the air saturation value at 21.0C. The test duration was 48 h. Current control chart for D. magna acute testing is included in the raw data package. Sponsor 3M Company Sponsor Study ID# R1335 5 001365 AScI Corporation/AScl-Duluth Environmental Testing Division AScl Study ID# 5010468 5.0 REFERENCES NAmerican Public Health Association (APHA). 1995. Standard Methods for the Examination of Water and Wastewater. APHA, Washington, D.C. Hamilton, M .A., R.C. Russo, and R.V. Thurston. 1977. Trimmed Spearman-Karber Method for Estimating Median Lethal Concentrations in Toxicity Bioassays. Environ. Sci. Technol. 11(7):714-719; Correction 12(4):417 (1978). Organization for Economic Cooperation and Development (OECD). 1993. OECD Guidelines for Testing of Chemicals. OECD Publication Information Center, Washington, D.C. .'-U University of Wyoming. TOXSTAT Package Version 3.1. , , Sponsor: 3M Company Sponsor Study ID# R1335 6 001366 AScI Corporation/ASct-Duluth Environmental Testing Division AScI Study ID# 5010-068 TABLE X. ACUTE TOXICITY O F R1335 TO DAPHNIA MAGNA TestSubstance Concentration 0 130 216 360 600 I 1000 * Percentages are in parenthesis. Cumulative Number and Percent of Dead and Immobilized Organisms* 24 h 48 h 0 (0) 0 (0) 0 (0) 0 (0) 2 (10) _____________ i m _____________ 0 (0) 0 (0) 0 (0) 1 (5) 10 (50) i3 (65) I ;1 EFFECT AND NO-EFFECT CONCENTRATIONS WITH CORRESPONDING 95% CONFIDENCE INTERVALS 1 EC50 and NOEC 1 24-h ECJ0 I 48-h ECS0 1 48-h NOEC Data (mg/L) >1000 653 360 95 % Confidence Interval Not Calculable 396-1077 Not Applicable Sponsor: 3M Company Sponsor Study ID# R1335 7 00136