Document O1MLqj1w8791vgv8NYxM0O0RK
/|R 1%-OS07
ACUTE TOXICITY TO AQUATIC INVERTABRATES (DAPHNIA MAGNA)
TEST SUBSTANCE
Identity:
Perfluorooctanoic acid, ammonium salt; may also be referred to as PFOA ammonium salt, Ammonium perfluorooctanoate, PFO, FC-
116, FC-126, FC-169, or FC-143. (Octanoic acid, pentadecafluoro-
, ammonium salt, CAS # 3825-26-1)
RIt'esmpuarriktys:wasTnhoet 3suMffipcrieodnutlyctclhoat rnaucmtebriezrewd,atsho3u7.ghThcuertreesntt sinafmorpmleatiisonFC-143. ainnddicCat9epseirtfliusoaromaixntaulroegoufe9c6o.5mp-1o0u0n%ds.test substance and 0 - 3.5% C6, C7,
scTtohumedfipeolesllothewadivnDegabpieshenanniasaumbpabegrrencvaeiadsteteudddbiseyusamcmmonoadrreyucroetfecsdeentvotetereavslatpluraetveioduiesltyThese
METHOD:
TYGMyeLepaPther::ocdoNSm:otaptliecteaNdco:utt1ne9o8te2d.
SSATEepuxnspepatcoplyoisletireuiscgrr:aa:elDnUpmiasSepmorEhinoPnaidiAtago:-erEm4i:nR8aFg-Lghi:,rnosTaDute-ruIsmnlusptthea,rraMytouNur.en,gp(H0.a5n-1d.5dimssmo)lved oxygen SPcDotriolmeuctmtrkieooaanntnmwsdetaotntecetsk:rt:ssNCooloaultrutbinotoionont,en-f5dipl.tger/erLepdarwaetilol nw:atTeer.st solutions were created by a CScEmtoxapnbdoceilesiptuntyhrtre)oa. fvtiteohsnessetedlsso:tsci2nh5ge0mrmaitcLea:gllOsaosnscluebtieoankse:rsNcoot nntoatiending 200 mL test solution (6 Number of replicates: two
Number Number
of of
dcoapnhcennidtrsaptioernsre: pfilviceaptelu:s1a0b- l2a0nk
control
RESULTS
Element values: 48-hour EC50 = 126 (86-183) mg/L to >1000 mg/L Element values based on nominal concentrations
003811
CONCLUSIONS
Tbinehceo>n1tes0si0stt0esanmmt.gp/LVle.alrTueehsseulgvtsaenfluoeerra4gt8ee-ndheoirnuartMeEaCiny5oJ0 uffon1re9D8o2af pd1he9n8tei2armimnidnaiegcdnaat4e8wd-eharoe4u8r-hEoCu5r0S to iEnCco50nsoifs1te2n6cimesg/mL awyithbead9u5e%tocodnifffeidreenncceesInintedrvieatl.of 86 to 183 mg/L. These
SPuaublm, Mittinenr:eso3tMa,
Company, 55133
Environmental
Laboratory,
P.O.
Box
33331,
St.
DATA QUALITY
Reliability: Klimisch ranking 3. The method is not described, sample purity wsoutahbsesrnt.aonTtchpeerocppouenrrcplyeoncstheraaortiafoctnhtsee.rsizeTehsdteuadvnaiedlusietwslaafcsrkotsmoaemnvauallyltuitpiaclteaeltdeciosffntesfrirecmonntattdrioaandpihcontfiedteasdctihets.
REFERENCES
These studies were conducted by the 3M Company, Environmental Laboratory, St. Paul, MN, completed from May to June, 1982.
OTHER
Last changed: 5/25/00
003812
ENVIRONMENTAL LABORTORY AQUATTC TOXICITY DATA S'-'EET
Tyre Test<
t c CO
M ater4al Tested f t W 3
Lot U L
Test Organism \J.
Source O o fu tt ffc L Oat? Rec'd I t jt jf f iatch No. -
Age: First-Instar young (0,5-1.5mm) No. Organisms per treatment^-& < L ________
Test Chamber: 250ml glass beaker Test Solution Vol.: 200ml Test Solution Depth: 6cm.
Time Started /0:3<g ( O f f * Pate Started 5/2U Z.
Date Completed
Dilution Wfter Carbon-filtered well water CTemp. Q jf* C, D .0.
ppm , pH_
.
j
Investigator (Full Signature)
^ (95% Confidence Limits)___ __
fit S
Tank No.
Control
Symptoms or Reactions
24-Hr.
Total No. Affected
48 Hr.
72-Hr.
96-Hr.
Comments:
------------------------------- BEST COPY AVAILABLE
0Q3S13
ENVIRONMENTAL LABORTORY AQUATTC TOXICITY DATA S'-'EET
Tyre Test
Material Tested F c / h s
__ Lot
Test Organism
Source P M i R L Date Reo'd {S/fj/J&iatch No._______ __
Age: First-In star young (0,5-1.5mm) No. Organisms rer treatment 10
Test Chamber: 250ml glass beaker Test Solution Vol.: 200ml Test Solution Depth: 6cm.
Time Started^ ?*>/
___Date Started
Date Comnleted|
Dilution Wrter Carbon-filtered well water (Temp.
C, D .0.
ppm , p H )
Investigator (Full Signature)
Init. ank Cone.
N o . L.mg/1
c Control
- 1* l 3*..
Uo
1 3m a
Total Amount Added
2 4
(90 2O
___ L<^ 5 0
Confidence limits) _______
fed
24 Hour s
Total
D.O.
No. Dead pH ppm
O /tG
*Jto
48 lours
Total
D.O.
No.Dead pH ppm
S7 9 X Of/O 8.7 $>z.
Vl6 ? ,7
y to f t ? .Z
fir?V /a
Y fo
8 f7 g .Z , Z .!
-73-Hour s
Total I D.O. No.Dead pH ppm
<v~ V /
V , V ,o > K Z Y/o
AA'k. >
-96-Hou :s
Tot&I
D
No.Dead >H Pi
/ 'O 1h *1
V /tf / t l s
Li 8
V/ *
% > /
I * f
Test Temp. C. Initials S Date
A rK /tV
& '?S d 'L-
Coraments
XL. SlvtAA*
Effects other than Mortality
EC^
A t) tr rift*-
2M 6
*
Tank No.
Control
Symptoms or Reactions
24-Hr.
Total No. Affected
48 Hr.
72-Hr.
96-Hr.
3SI1] ii1:17:11.'']1 1
Comments :
003814
ENVI ROVENTAI. LABORTORY AQUATIC TOXICITE DATA Sf-rEET
Tyre Test V^i..rCjr
Material Tested
/ 1/3
Lot 3 7
Test Organism
uo<- Source fb ltd i 3L
Dat Rec'd f2/;ylf/Batch No._______ __
Age: First-Instar young (0,5-1.5mm) No. Organisms per treatment 2 O
Test Chamber: 250ml glass beaker Test Solution Voi.: 200ml Test Solution Depth: 6cm.
Time Started. / ( J O M l
Date Started S Z l'L
Date Completed b f z J f i Z )
Dilution Wrter Carbon-filtered well water (Temp. 2
C, D 0.
ppm > pH____ _J
Investigatcr f 3 A ^ c ^ fyU /eS & t
L C ^ (95% Confidence lim i t s ) _ _ _______
(Full Signatura}
ank No.
Init.
Cone. _mg/l
C Control
t 10 lo o OO
r /coo
Total Amount Added
'
a.
030o 3.00
24 Hours
Total
D.O.
No. Dead pH ppm
O
0
H1
/I
48 Hours
itfffT l Hour S
Total
D.O. total I D.O.
No.Dead pH ppm No.Dead pH ppm
Z/ 1 d
i 02 j
f
I.f
<T7-
1
5
X !' 11
Hou Total
:s
C
No.Dead >H p
f) tu ip
2 -
Test Temp. C. Initials S Date Comments :
Z J J '/ f
Effects other than Mortality
E^50-
Tank No.
Symptoms or Reactions
Control Z 3
S ____
____ .
fe d i/e fro *.-ia c n
24-Hr. 3
Total No. Affected
48 Hr.
y --Hr.
9,
1 r ...
1r
73
YJiS^Hx.
Comments :
BEST COPY AVAILABLE
C03S15
I
T'$r*\
1'
0
'
0
'5H0--
_
7 1 0 ij*c!0 j 1
7' 5 0 0 ? 0 ?5
? 100 0 j 0 j 17
- *j'i'.Ja *Z.:r Ll *Zi. * '
COMMENTS FOR CURVE 4 7 iz!4 HR RUN E
COMC .
LOG CONC.
FRACTION RESPOND ING
i0
100 5 00 100o CHI--SO
1 c dm 69897
PRDBRBIL ITY IS >
0, 05
ij
0. 05 0. 5 0.85
NO OF ITERATIONS
INTERCEPT <R>
SLOPE <;
r*
--7.61349
. 7 4 7 3 08
H E T E R O G E N IET Y FACTOR <HV = 1
LC-50 =
589981
95 PCT. LOWER CONF. LIMIT -- 95 PCT. UPPER CONF. LIMIT =
.'.Sr:
NO. DF CONC. !FOR CURVE 5 7 --j INPUT D A T A - C D N C . j ND. EXPOS ED? NO. D E R D
? 1 0 ? LiJ *;'* 1 Ijl_liz!U ,i8 ? 5 0 0 ? ? 0
CO M M ENTS FOR iCURVE 5 ' 48 HR RUN :E
CONC.
LOG CONC.
FRACTION RESPONDING
10 1
1 1j0 c. -
5 00
.69897
CHI-SG! P R O E R B I L I T Y < 0.05
0. 1 0.3 1
BEST COPY AVAILABLE
FITTED FRACTION
5.788753E-07 0. 017 0985 0.4 17381 0.7355148
CHI SO FDR LINEARITY
CHI SO FOR PROEREIL ITY
5.054567
0.07987569
-i.
.-G = 0.66042/
4 04.5 14 861.9653
FITTED FRACTION
0.03887939 0.509768 0.8987583
NO OF ITERRTIONS
INTERCEPT <R>
SLOPE
CHI SO FOR ^ V CHI SO FOR
LINEARITY
PROBABILITY
6
-3.55151
1.78838 1
7.774071
0.0053 00149
H E T E R O G E N IETY FRCTOR <H> = 7.7 74 u 1
b = 8 8 8 c*5 1'"1
SINCE b IS G R E ATER THRN 1 ? THE CONF. LitjITS ARE NOT D E F I N E D
C i_-- s ii --
9t'i by5*9
95 PCT LOWER CONF. LIMIT 95 PCT UPPER CDMF. LIMIT
00
C03S16
iVfr'i PPL
M ENV I E Dh MENT AL L A> F A IU ^ v
P R O B IT ANALYSIS FDR BIDASS AY DATA***
ND DP CURVES 5
ND. OF CDNC. FDR CURVE 1 7 4 INPUT DATA-CuNC. >NU. EXPOSED tN U .DEAD
" 1 Cij 0 j 0 ? 10 0 * 0 j 1 ? 5 ij0 j 0 j4 7 1 0 00 j 0 >17
COMMENTS FDR CURVE 1 ? 4 HR RUN Ft
CDNC.
LOG CDNC.
FRACTION RESPONDING
FITTED FRRCTIDN
10
100 5 00 1000 CHI-SQ
l'
c.
.69897 y
PROBABILITY < 0.05
0 0.05
0. , 0.85
.821752E-07 -0 . 0 1 3 4 2 6 2 -i
.>"'0.3959862 - . 0.7177 021 ;
V. 4 UNDERFLDLi RT L I N E O'!550 DF M R I N P R D G R R M
ND OF ITERATIONS
f
INTERCEPT CRj
-- 7.793 Out
SLOPE <B>
. 7 8 9 1-'uy
CHI SO FDR LINEARITY
6.95989
CHI SO FDR PROBABILITY
0.03081836
H E T E R O G E N IETY FRCTDR <H> = 3. 479644
G = 5.140073
SINCE G IS GREATER THAN 1, THE CDNF. LIMIT S ARE NOT DEFINED
LC-50 =
621.606
95 P C T . LONER: CDNF. LIMIT = 95 PCT. UPPER CDNF. LIMIT
0 0
NO. DF CDNC. FDR CURVE T, -.[
INPUT D A T A - C D N C .?N D . EXPOS ED ND. DEAD
'' 1 0 > iJ> 7 100j 0f6
;
? 500 20* 20
COMMENTS FOR CURVE : ? 48 HR RUN A
CDNC .
LDG CDNC.
FRACTION RESPONDING
10 1 100 c 5 00 .69897 CHI--SU PROERE ILITY < 0.05
0. 1 0.3 1
,/
/ V, . ' 7-'6: .4,- If
/
*'
FITTED FRACTION
V 4
0.03887939 0.5097682. u ysy f c
,
ND DF ITERATIONS
INTERCEPT `.A>
SLOPE (E>
CHI SO FDR LINEARITY
CHI SO FDR PROBABILITY
&
-3.552151
1.788331
(*'1*'('4*U I*'l-1
0.00530014
Hb.T E.ROGE NI El Y FACTOR (R.i = 7. 774071
b -- 3282.571
SINCE G I S GREATER THRU 1 , THE CDNF. L I M I T S ARE.NOT DEFINED
003817
,r 'RUN PR DB IT
3 M E N VIR N M E N T ftL L ftB D ftftTDR V
P R O B IT fthftL Vi-1 > F O R BIDftvSftY DftTft**"*'*
r-H OF CURVES ~ 1
-0 , OF C D N C . FDR CURVE i ? 3 IN PU ! BftTft-- LONG. ?H D . EXPOSED.* NO. DE PI*
? 1 0 -40?4 i' 1 LIi.i.4 IJ;ic! ? 5 0 0 *4 0 ?4 0
CONHENTS FOR CURVE 1 ? C O M B I N E D 48 HR RUN ft&B
CDNC.
LDG CDNC.
FRRCTIDN RESPONDING
10 1
100
5 00
. ft.9897
C H I-SQ PROBftBILITY < 0.05
0.1 0 .3 1
BEST COPY AVAIl ABLE
FITTED FRACTION
0.0388795 0 .5 U97 ft'fc'9 0 .8987511
NO OF ITERftTIDNS
INTERCEPT t.ft>
SLOPE LB>
CHI SO FOR L INEARITY
CHI SQ FDR PROBftBILITY
ft
-3 .5515
1 r'yyjc*
15.54809
8 .045137E-05
H E T E R D G E N I E TY F PC TOR' (H-1 = 15,54809
G = 3 8 . 549
SINCE G IS G R EATER THftN i. T H E CDHF, LI M l S ARE NOT DEFINED
LC-50 =
9 ft.89595
9 5 P L T . L 01.0E R CDNF. LIMIT cr 95 PCT. UPPER C O N F . LIMIT 5=
0 o
0.03818
V 5 3i
EFA P R O B I T A N A L Y S I S P R O G R A M
USED FOR CALCULATING EC VALUES Version 1.4
FC-143 Daphnia magna acute static 48~nrs exposure
Cone .
Number Exposed
Number Resp.
Observed Proportion Responding
Ad jus t e d Proportion Responding
10.0000 100.0000 500.0000 1000.0000
40 40 42 40
4 0.1000 12 0 . 3 0 0 0 42 1.0000 40 1.0000
0.1000 0.3000 1.0000 1.0000
Predicted Proportion Responding
0.0306 0.5327 0.9262 0.9792
Chi - Square Heterogeneity = 19.408
^ ^ ^ ^ ^ ^ ^ ^ ^ ^ ^ ^ ^ ^ ^ ^ ^ ^ ^ ^ ^ ^ ^^ ^ ^ ^
^ ^ ^ ^ ^ ^ ^ ^ ^ ^ ^ iTl **
^ ^ ^ ^ ^ ^ ^ 4, 4^ ^ ^
U
^ ^d r ^d r ^d r ^^ fd r ^4 ^Ur ^dr ^4 W^ ^^ ^^ ^ ^ ^ ^^ ^ ^4 *Ur ^^ ^ ^ ^^ ^ ^ ^4 ^ ^d r 4f ^Ur rdjrl
* WARNING
*
**
* Significant heterogeneitv exists. The results reported %
* for this data set mav not be valid. The results should #
* be interoreted with appropriate caution.
#
v tI
^^
^
rfv ^ t ^ ^ ^ ^ ^ ^ ^ ^ ^ ^ ^ ^
^ rf^ ^ ^ ^
^
^
^^^
^^^^^
^ ^ ^ ^1 ^1 ^ ^
^^^^
J. ^ 4 ^ d r ^ 4 ^ 4 ^ ^ ^ 4 ^ ^ d ^ ^ ^ ^ ^ 4 4 ^ ^ 4 ^ ^ ^ ^ ^ 4 ^ ^ ^ 4 r ^ 4 U r ^ ^ 4 ^ d r d 4 * ^ 4 W ^ 4 ^ d r 4
4 Ur ^ Ur Ur ^ 4 4
*v*v*
^ ^ ^ * ^ rp r^
^ r ^ ^ ^ ^ ^ ^ ^ ^ ^ ^ ^ ^ ^ ^ ^ r ^ r ^ ^ ^ ^ r p ^ ^ ^ ^ ^ ^ ^ m 'm ^
^ ^ ^ ^ ^ ^ f ^ ^ jf> ^ ^ ^ ^ ^
** ,. N O T E
**
* SloDe not significantly different from zero. * EC fiducial limits cannot be computed.
# #
* ^^U ^t ^ ^4 ^^ 4 r ^d r *4 ^* ^ ^4 ^U^ ^U r ^U ^^ ^4 rrf^r r^ ^ ^d r ^4 ^^ ^4 ^^ ^4 ^4 ^\ k ^^ ^4 ^1^ ^4 r ^U r ^W ^4 ^U r ^^ rU^ ^iV U r ^U ^U r ^ ^U r ^U ^U r ^^ ^U r ^U ^U ^W ^1^ d^/ ^4 ^U ^Ur ^U r ^Ur ^^ 4^ ^4 ^^u ^4 ^4
Mu 1.958058 Sigma = 0.511587
Parameter
Estimate
Std. Err.
95% Confidence Limits
Intercept Slooe
1.172584 1.954700
1.712521 0.772273
( -6.196393, ( -1.368389,
8.541559) 5.277789)
Theoretical Spontaneous Response Rate 0.0000
C03S19
B U RL IN GT ON RESEARCH. INC. TRIMMED SPEARMAN-KARBER METHOD.FOR CALCULATION OF EC50 AND LC50 V A LU ES IN BI OA SS AY S
FOR REFERENCE. CITE M .A . H A M I L T O N . R.C. R U S S O , A N D R .V . T H U R S T O N , TRIMMED SPEARMAN-KARBER METHOD FOR ESTIMATING LETHAL CONCENTRATIONS IN TOXICITY BIOASSAYS. ENVIRON. SCI. TECHNOL. 11(7) 714-719 CORRECTION 12(4) 417 (1978).
1977. MEDIAN
D A T E _____ T E S T # ___
._15 / 3 1 / 8 2
CHEMICAL.
.FC-143
SPECIES_
_Daphnia magna
DURATION
48 hours
RAN DATA
C O N C E N T R A T I O N (m o / L )
1 0 , 00 100.00 5 0 0 . 0 0 1000.00
NUMBER EXPOSD
40 40 42 40
MORTALITIES
4 12 . 42 40
SPEARMAN-KARBER TRIM
10.00
SPEARMAN-KARBER ESTIMATES
eC50
125.7965775
957. L O W E R C O N F I D E N C E
86.29
UPPER CONFIDENCE
183.39
003820
BURLINGTON RESEARCH. INC
T r i m m e d S p e a r m a n - K a r b e r M e t h o d -for C a l c u l a t i o n ot EC50 and LC5 Values in Bioassavs
6.907E 3 3 3
X
3
6.044
3
3
3
3
5 . 180E
L3
N3
3
C3
G 4.317E
N C
33
E3
N3
T 3.453E
R3
A3
T3
0I 3 2.590E
N3
3X
3
3
1.726E
3
3
3
3
.S 6 3 4 E
3
3
3
3
X
X
gdddddeddddddddeddddddddddddddeddddbdddddddddedddddddddddddde
LC10
LC25
LC50
LC75
LC100
MORTALITIES
C03821
FC-143 Daphnia magna acute static 48-hrs exoosure
PLOT OF ADJUSTED PROBITS AND PREDICTED REGRESSION LINE
Probit 10+
o
9+
o
8+
/+
--
.5+
4+ --o
3+ a
B
BSB
BB
M B 8BB
BBB
abaa
B
abb BBBB
BBBB
baa
O
BB 8a BB
aaba
a
1+
0+ E C 001
E C 10
EC25
EC5C
EC75
EC90
003822
EC
FC-143 Daphnia magna acute static 48-hrs exoosure
Estimated EC Values and Con-fidence Limits
Point
Cone.
Lower
Upper
95% Con-fidence Limits
EC 1.00 EC 5.00 E C 10.00 E C 15.00 E C 5 0 .00 EC85.00 E C 9 0 .00 E C 9 5 .00 E C 9 9 .00
5.8607 13.0782 20.0635 26.7824 90.7942 307.7991 410.8741 630.3306 1406.5795
003823
