Document O15RQY9njkQpbXJLJQK53rvEe

ACUTE TOXICITY TO AQUATIC INVERTEBRATES - Daphniamagna TEST SUBSTANCE IdentityN:-Ethylperfluorooctanesulfomnaaymaildseo;be referrteodas U 1464, ETFOSA, ETPOSA, F-6309, or FX-12. (1Octanesulfonamide,1,1,2,2,3,3,4,4,5,5,6,6,7,7,8,8,8heptadecafluoro-N-ethylC-A,S # 4151-50-2) Remarks: Materialisa whitesolid.Sample purityand lotnumber were not recorded. METHOD Method: Adapted from OECD 202 - Water accommodated fractions Type: Staticacute GLP: No Year completed: 1998 Species: Daphnia magna Analyticalmonitoring:Nominalconcentrationosnly.Temperature,pH and dissolvedoxygen recorded Statisticamlethods: :EL50 (lethalloading)valueswere calculatedusing the probitmethod. The NOEL was calculatedby Fisher'sExact Test, TOXSTAT Software Package, Version3.5,Laramie,Wyoming. Test daphnid source:.ASci in-housecultureD,uluth,MN Test daphnid age atstudy initiation<: 24-hourold neonates Test conditions: Dilutionwater: Carbon-treateddechlorinatedCityofDuluth,MN tapwater aeratedfor24-hours priortouse inthetest. Dilutionwater chemistry during the study: Hardness 44 mg/L as CaC03 DO: 8.1-8.9mg/L Alkalinity: 42 mg/L as CaC03 pH: 7.9-8.2 Conductivity 113-125 pmhos/cm Test Temp. Range: 21.3-21.5 Stock and testsolution preparation:Test solutionsprepared by mass additionoftestsubstance to 2.OLaliquotsofdilutiownater (one per concentration)T.he solutionwsere vigorouslystirred (vortex1/3to 1/2solutiodnepth)for18.5hours. The aqueous phase was siphoned from the vesselatmid-depth. Exposure vessels: 250 mL borosilicatgelassbeakers Exposurevolume:200 mL Number of replicatesf:our Number ofdaphnids per replicate5: Number ofconcentrationsF:iveplusa negativecontrol Element basis:mortality RESULTS Nominal loading concentrations: Bk control,5.8,9.6,16,27, and 45 mg/L Element value: 24-hour EL50: 24.6 (21.3-28.6)mg/L 48-hour EL50: 14.5 (12.1-17.4)mg/L 48-hour ELo: 7.3(5.0-9.2m)g/L 48-hour NOEL: 5.8 mg/L Mortalityof controls: 0% after48-hours exposure Percent Mortality Nominal Loading Concentration,mg/L 24-Hours 48-Hours Negative Control 0 0 5.8 0 0 9.6 0 35 16 15 50 27 50 85 45 100 100 Remarks: Allvalues reported are for nominal loading concentrations. No measurement of testconcentrationswas conducted. CONCLUSIONS The testsample 48-hour EL50 forDaphnia magna was determined to be 14.5 mg/L witha 95% confidence intervalof 12.1 - 17.4 mg/L Submitter: 3M Company, Environmental Laboratory,P.O. Box 33331, St.Paul,Minnesota, 55133 DATA QUALITY ReliabilityK:limischranking2. This study,whilewellconducted using the methodology acceptableat the time,lacksanalyticadlata for determinationof the testsubstance concentrationinthe testsolutionsand determinationof the sample purity.Testingwas alsodone wellabove the solubilitlyimiotfthetestsubstance withthe use ofwater accommodated fractions. REFERENCES Study conducted atthe requestof3M Company by,ASci Corporation, Duluth,MN. OTHER Submitter: 3M Company, EnvironmentalLaboratory,P.O. Box 33331, St.Paul,Minnesota,55133 Last changed: 5/18/00 I )t ASci Corpomtion/ASci- Duluth Environmental Testing Division Submitted to 3M Co. - 5198 ASci Study ID# 50IG-068 STUDY TITLE ACUTE TOXICITY OF U1464 TO DAPHNIA MAGNA DATASTANDARD OECD GUIDELINE 202 STUDY COMPLETED May 20, 1998 TESTING FACILITY AScl Corporation/AScl-Duluth Environmental TestingDivision 4444 Airpark Boulevard Duluth,MN 55811-5712 Tel.No. (218)722-4040 Fax No. (218)722-2592 STUDY IDENTIFICATION'NUMBERS AScl Study ID# 5010-068 3M Company Lab Request# U1464 Spcnsor: 3M Company Spc,nsorStudy ID# U1464 ASci Corporation/ASel-Duluth Environmental TestingDivision Submittedto 3M Co. - 5/98 AScl Study ID# 5010-068 1.0 GENERAL INFORMATION @;tudyTitle Data Standard ,I;ponsor I;ponsor'sRepresentative '3ponsor'sLab Request# 'I'estiFnagcility PrincipalInvestigator ProjectManager TestingFacilityDirector DefinitiveTest Dates Data Validationand Report Review Acute Toxicityof U1464 to Daphnia mag OECD Guideline202. 3M Environmental Technology and Services, Building2-3E-09, 935 Bush Avenue, St.Paul, MN 55133. Rochelle R. Robide-au,3M Environmental Technology and Services,Building2-3E-09, 935 Bush Avenue, St.Paul,MN 55133. Tel No. (612)778-7065. U 1464. AScl Corporafion/AScI-DuluthEnvironmental TestingDivision,4444 AirparkBoulevard, Duluth,MN 55811; Tel.No. (218)722-4040. Todd McGuire. Joe Amato. Donald Mount. April28-30, 1998. Alan Mozol. Report Signature, Retentionof Raw Data and FinalReport Locationof Raw Data and Final Report Two yearsfrom the date studyiscompleted. AScl Corporation/AScl-DuluthEnvironmental TestingDivision,4444 AirparkBoulevard, . Duluth,MN 55811; Tel.No. (218)722-4040 S,L:>onsor3:M Company S?onsor Study ID# U1464 2 AScl Co rporation/AScl-D uluth Environmental Testing Division Submittedto 3M Co. - 5/98 ASci Study ID# 5010-068 2.0 OBJECTIVE(S) To determinethe48-h EL's (effectivleoading'sE)LIO and EL505 and 48-h NOEL (no observed effectloading)of thetestsubstance,U1464, forDaphnia magna. Iiisstudy was conducted accordingto the ASCI studyplan retainedby the Sponsor and OECD iuidelin2e02 (OECD 1993). Test solutionpreparationwas water-accommodated fraction @ C(WAF) per Girling& Whale 1994. 3.0 TEST METHODS - @"estSubstance Test Organisms DilutionWater Exposure Chambers Properties: U1464, white crystals,specificgravity 1.7 (water = 1), stable, and negligiblesolubilitiyn water. Test loadings:0 (dilutiownater control)5,.8,9.6, 16, 27, and 45 mg/L. Each testsolutionprepared by mass additionof testsubstanceto 2.0-L aliquotsof dilutionwater. Each solutionwas vigorouslystirred(vortex1/3 to 1/2 of flasks'depth)for 18.5 hoursto obtainWAF'S. Test solution obtained from flasksby siphoningaq"ueousphase from mid-depth. Source: Healthy, < 24-h old Daphnia magna neonatesobtainedfrom AScl mass organism culturesmaintainedin dechlorinateCdity of Duluth tap water. Use in test: Gravid adultsisolated24 h before testinitiationU.sed 5 organisms/repilcateand 4 replicates/telsotading. Did not feed duringthe test. Source: Carbon-treatedechlorinateCdity of Duluth,MN, tapwater. The most recentchemicalanalysisof postcarbon (PC) water isin the Raw Data Pa ekage. Characteristics:At testtime, the water had a hardness of 44 mg/L (as CaC03) and a pH of 7.99. Aerated for24 h priorto usingin the test. 7@pe: 250-ml borosilicatgelassbeakers. Use in test: 200 ml of dilutionwater or testsolution/chamber. During the test,kept covered, except when experimentalobservationswere made. Sl>onsor:3M Company Sponsor Study ID# U1464 3 ASci Corporation/ASci-Duluth Environmental Testing Division Submitted to 3M Co. - 5/98 AScl Study ID# 5010-068 Iricubation Cibservations E,ndpoint Calculations Duration: 48 h. Dailyphotope7lod: 16 h lightand 8 h dark, maintainedusing cool-white fluorescenbtulbs. Temperature: 21'C. Biological:Daily- counted dead testorganisms. Did not remove any daphnids from the exposure chambers at 24 h. Water chemistry: (1)At testinitiationt-otalhardnessand alkalinity(,2) daily-temperaturep,H, DO, and conductivity.All determinationswere made accordingto APHA methods (1995). ELIO and EL50: EPA Probit Method. NOEL: Fisher'sExactTest (TOXSTAT statisticsaolftware,Version3.5, Universityof Wyoming & West Inc.). 3.0 RESULTS I"estSubstanceToxicity(95% Confidence IntervalP)ertinentData arein Table 1 ]Zangesof Water Chemistry Parameters 24-h EL50: 24.6 mg/L (21.3-28.6mg/L). 48-h EL50: 14.5mg/L (12.1-17.4mg/L). 48-h EL,O: 7.3 mg/L (5.0-9.2mg/L). 48-h NOEL: 5.8 mg/L. Totalhardness (as CaC03 mgll): 44. Alkalinity(as CaC03 mgll): 42. Temperature ('C): 21.3-21.5'C. DO (mglL): 8.1-8.9. pH: 7.9-8.2. Specificconductivity(WMoslcm): 1.13-125. Sponsor: 3M Company Sponsor Study ID# U1464 4 ASci Corporation/AScl-Duluth EnvironmentaTlestingDivision Submittedto 3M Co. - 5/98 ASci StudyID# 5010-068 4.0TEST QA/QC Q/@/QCCriteria Testorganismdead,immobilizedor trappedin coiitroelxposuremust not be more than 10%. Thre lowestDO concentratioant test [t[tQTcTsaoeht]rminatiomnust be atleast60% of theair saturatiovnalue attesttemperature. Testdurationmust be 48 h. Referencesubstancetoxicittyest(thistestis optional). Data 0% dead,immobilizedor trapped. The lowestDO concentratiomneasured was 91% of the airsaturatiovnalueat21.0'C. The testdurationwas 48 h. CurrentcontrolchartforD. magna acute testingisincludedin the raw datapackage. 5.0 REFERENCES American PublicHealthAssociation(APHA). 1995. StandardMethods fortheExaminationof Water and Wastewa ter.APHA, Washington,D.C. GirlingA,.E.,G.F. Whale, and D.M.M. Adema. 1994.A GuidelineSupplementfor Dt-.terminitnhge AquaticToxicityof Poorly Water-SolubleComplex MixturesusingWaterA(.,commodatedFractionsC.hemosphere, Vol. 29, No. 12, pp. 2645-2649. Oi-ganizatifoonrEconomic Cooperationand Development (OECD). 1993. OECD Guidelinesfor Tc@stinogf Chemicals.OECD PublicatioInnformationCenter,Washington,D.C. Uj-llversiotfyWyoming & West Inc. 1996.ToxstatVersion3.5.Cheyenne, Wyoming 82001. L USEPA ProbitAnalysisProgram. Version 1.4. Sponsor: 3M Company Sponsor Study ID# U1464 5 .j ASci Corporation/ASel-Duluth EnvironmentalTestingDivision Submittedto 3M Co. - 5/98 ASel Study ID# 5010-068 T@LBLE 1. ACUTE TOXICITY OF U1464 TO DAPHNIA MAGNA Test Substance Lo.adhig ng@ L) 0 5.8 9.6 16 27 45 Cumulative Number and Percent of Dead Organisme 24 h 48 h 0(0) 0(0) 0(0) 3 (15) 10(50) 20(100) 0(0) 0(0) 7 (35) 10(50) 17 (85) 20(100) Ilercentagesare in parenthesis. EFFECT AND NO-EFFECT LOADINGS WITH CORRESPONDING 95% CONFIDENCE INTERVALS EL's.:@md:NOEL 24-h EL50 48-b EL50 48-h ELIO 48-h NOEL Data (mg/L) 24.6 14.5 7.3 5.8 95 % Confidenr-eInterval 21.3-28.6 12.1-17.4 5.0-9.2 Not Applicable SI)onsor:3M Company S])onsorStudy ]D# U1464 6