Document O13RJO3vbRdoedjBg8b4yaELX

Gril Company ASAI 6LAS5 0.170. MR 277 Y35-- [Company sanitized ows ACC Asai GlsCo,Lit Does not contain TSCA CBI. ssawaursscow 10.Goikaigan, Iehibarahi, Coiba 290-8566, Japan Characterization of a Polymeric Product (AAA) by Asahi Glass Study director (Question to:) To<uguh-idL e Ia scmm uraan) Senior Manager QAunaalliyttiycAalssTuercahnncoelOofgfyicGeroup Chemicals Company, AsahiGlassCo,Lid. 10, Goikaigan, Ichihara-shi, Chiba 290-8566 Japan Phone: +81.436.23-3183 Fox: +81.436.23.3126 E-mail: tsuguhide-isemura@agecop July 05D,at2e004. = = oon No monE * i fz 2m nox ou &So Company Sanitized LEE ig fle if 41h bE H e7f 04 H 5 ELh E] iz iis ed ! | VEERE 4 1 | | :. P111Es1d3 H5H. | gl| e g|s |1g HH l dI k a .a i EH S10. ei gf er | | "iH fii aeiErge, i Bs82 8: Bas Lp[a | !|Is iE ii5% :| z hEoie, zs F| EER Y IE Si p I8i||F2gbidH i iEEE kFBgRB off13 $53 HE IRL HE Chemicals Company, ASAHI GLASS CO.,LTD. 04705 L Substance name and TRP Identification# (for Noack shipment) ofthe polymeric product Substance name: AAA Lot. XXX (TRP Identification #: BBB) AAA Identification #. Lot. XXXX was purified for biodegradation test and named AAA CCC). The purification process and characterization of AAA purified were purified detailed (TR in the report" 2. Characterization of the polymeric product 2:1 Size exclusion chromatography (SEC) SEC of the polymeric product for molecular weight analysis was conducted according to OECD gwiutihdetlhienepsu'rifiaenddpotlhyemerres.ulHtewraesthsehoanwanlytiincatlhempertehvoidousalnyd sruebsumlitttaerde ormeiptorite"d ienxccleupstivfeorofthteheiscoolmatpiaorniasnodn preparation ofhe polymer from the produc, 2-1-1 Isolationof the polymer from the product product At first the polymeric product was and 5 mi of isopropanol was mixed ina shaken well for homogenization. 1 centrifuge tube and shaken well The ml of the polymeric precipitated polymer was separated by centrifugation (6,000 rpm, 10 min) and following decantation. The precipitate was then dried in vacuum condition for 20 hours in room temperature. The isolated polymer was dissolved in the mobile phase by 0.75 wi% by shaking for several minutes and stand for overnight, The solubility of the polymer was sufficient: no precipitation or insoluble fraction was observed and the solution looks completely transparent, 2:2 Determination ofPFOA in the polymeric product The method validation Attachment 1of tis report, and determination in this section was conducted according to the 22:1 Test and control substances standard, "The test substance, pefluorooctancic acid (PFOA, 98.9 &-hydrohexadecafluorononanoic acid (9H-PFNA, 97.85%, %, lot. lo. CIT001401), and the surmogate CS8002501), were purchased from Daikin Fine. Chemical (Osaka, Japan). Distilled water was produced in-house and checked for contaminants by LC/MS before use. Distilled water was filtered heough a pre-column, Preclean-ORG (GL Sciences (MeOH), Inc. (Tokyo, reagent grade Japan) ethanol placed (EIOH) between HPLC and isopropanol pump (IPA), and and auto-sampler. reagent grade HPLC grade methanol ammonium acetate were purchased from Kanto Chemical Co. In. ammoniumInaocretdaetretsoolauvtoiiodn cwoanstapmrienpaatrieodn,bydiwsepiogshaibnleg l1a5b4wmagreof(taubmems,onpiipuemttaec,eteattce) awnadsduissesdo.lv2inmgmionl1/1L of distilled water, `Chemicals Company, ASAHI GLASS CO, LTD. oars 2:2:2 Preparation of fortification standards distilled w50au/gte/tmhiaenaonlr)d. 11000mugg//mmll 8P-F2OalAcoshoollutsioolnutwiaonswparsepparreepdaruesdinugsiangsoilsvoepnrtop(a2n/o8l ((IwPiA)) fmoirxtsuorlveenotf for the use of later section (2-3). 22:3 Preparation ofa surrogate standard ethanol). 100 ng/ml 9H-PENA solution was prepared usinag solvent (1/1 (wt) mixture of istiled wate/r 2:2:4 Preparation of calibration standards each solut0i,o0n.a1n,0d.29,m0.o4fm1l0o0fntgh/eml1090Hu-gP/EmlNAPFsoOluAtisoonluwtaiosnmwiaxseddiilnutaedvibayl.IAPbAouutp t1om5lmo.f e1a0c0huslolouftitohne was filtered through a 0.2 yum filler, injected twice into LC/MS conditioned Milex-LG as follows. (MILLIPORE, U.S.A), ino an auto-sampler vial and 2:2:5 ExtractionofPFOA from the polymeric product to homogenBiezfoertehethpeoslaymmpelriicngproofdutchte.po1lmylmeorficthperpoodluyctmetrhicypwreordeucsthwaakesnpwuetllinweiatchhouotfgfeonuerrcaetnitnrgifbuugbabtlieosn vis. Fortification of PFOA and 8-2 alcohol were conducted as listed in Table 1. Fortification of 82 alcohol was conducted fo with fortification solution the was use of 5 mi. later After section puting (2-3). a Ji, IPA each was added into all of five vials (with vials uni otal the blank) was amount shaken vigorously and the precipitation ws separated by centrifugationat6000 rpm for 10 minutes, About 3 ml of each supematant was dealt out fo 8-2 alcohol determination by GC. About 1 100 ul of ml of each the cach solution supernatant was filtered and 9ml through of the 100 0.2 ng/ml SH-PFNA solution was um fir into an aut-samplr mixed in a vial. vial and injected into LC/MS conditioned reproducibility. as follows These procedures were repeated once again for the verification of the "Table 1 FortificationofC8 precursors and PFOA Sample Fonification CL 0mpmis2acol moa Ta ou Cow B soul 10001X 50 ug/ml c 100 100 WX 100 g/m 200m 200 1 X 100 pgm 22:6 LOMS analysis LC/MS analysis ws conducted with the conditions listed below. Chemicals Company, ASAHI GLASS CO, LTD, 040705 <Instruments> (yLems HPLC Mass spectrometer (2)LC column @rc (@) Software (5) LOMS vial Agilent Technologies, Model 1100 Agilent Technologies, Model 1100MSD (SL) Imtak (Tokyo, Japan), cadenza CD-C128 mm (diameter) X 100 mm Hewlett Packard Kayak PC Workstation OS: MS-Windows NT 4.0 LC/MSD ChemStation, Rev. A. 08. 03 2m vial for auto-samplr, Chromatography-Convenience 400-C80, Tomsic (Tokyo, Japan) <Analytical conditions> (1) Column temp. (2) Mobile phase (3) Gradient condition @) Flow rate (5) Injection volume (6) Retention time sc A:2 mmol/L ammonium acetate B: Methanol time/min 05> 2 515 15015 25 BS ST3575 1005100557 557 0.3 m/min SL PFOA (4.3 min), 9H-PFNA (2.5 min) <MS operating parameters> (1) Tonization mode Electrospray (ESI) (negative ion mode) (2) Fragmentor voltage ~~ 100V. (3) Ton monitored miz =413 (CF15C00'), 445 (CEH(CF:),C00) (4) Desolvation temperature 350C (5)Nebulizergas N (6) Desolvation N: 12 Limin 22.7 Resulis Of (peak Twocalibration curves for area of mz = 413) / (peak standard area of addition method m/z = 445) as a are plotid and shown in vertical axis against the Fig. 1 for the ratio amount of PFOA faonrdtiftihceataimonou(nitgs) oasf aPFhoOriAzodnettaelramxiins.edThien ctoheeffpiocliyemnetsroifccporrordeulcattiaornea(reCBmIo)repptmhanan0d.9(9C9B0I)foprbpomth(cauvrevreasg,e (CBD ppm). (CBI) ppm is equivalent to (CBI) molkg (specific gravityofAAA is (CBI). Fig. 1. `Two PFOA calibration curves for is not detected (zero PFOA count) calibration standards are shown for 0 ug standard (blank). The in Fig. 2 for the coefficients of same axis with corelation are. more thn 0.9995, and the recovery defined as (th slope for the standard addition) / (the slope for the Chemicals Company, ASAHI GLASS CO, LTD. 040705 calibration standards) recovery satisfied the was 955. All the criteria stipulated linearity of the calibration in the attachment 1. curve, reproducibility of the resul, and (CBI) [C3] Fig.1 Calibration curves for AAA by standard addition method Fig. 2 Calibration curves for PFOA. calibration standards Chemicals Company, ASAHI GLASS CO, LTD. 040705 2-3 Deter"Tmhienmaettihoondovfal8i-d2aatilocnoahnodldaetnedrmiCn8atpiroencwuarssocrosndiuncttehde polymeric product according to the Attachment 1 of his report for 8:2 aleshol. C8 precursors other method us4cialibnratigon standards, and not than 8:2 evaluated alcohol were determined by by standard dition method, absolute calibration 2:31 Test and contol substances further "The test substances purification process. used in ths sction are listed in Table 2. These substances were used without Standards Table 2 The test substances used in 2:3 Origin Purity by GC 2 acon Reagent grade, purchased from Dakin fine chemical (A-1820 ot, A18202601) 82acrylte Received from Seimi Chemical Co,, Lu. (Kanagawa, JAPAN) 9.71 (FID area) 820lein Prepared in-house 99.75 (FID area) CO th iodide Reagent grade, purchased from Dakin fine chemical (11820 ot. 118200701) --CH -- iodide PPereppaerdediinn-hhooussee 8897.77((FDIDaamreaa)n 23:2 Preparation of fortificationandcalibration standards listed in Mixture Table 3, solutions of 8-2 alcohol and C8 precursors were prepared using IPA as 2 solvent as `Table 3 Preparation of 8-2 alcohol and C8 precursor standard solutions Standard _2alcohol 82acrylale 82olin C8 ethyl iodide g/ml IPA solution CS odide A B 10 10 10 EY 50 50 10 1 s0 5 -- X c> 3050000 0 300000 ss30o000w 0 s3sw000e00 33m500 2:3:3 Extraction ofC8 precursors rom th polymeric product Extraction proceduresof8-2 alcohol and C8 precursors are explained in 22.5, 23:4 GC analysis GC analysis was conducted with the conditions listed below. Chemicals Company, ASAHI GLASS CO, LTD, 00705 <Instruments> WGems Gc Integrator (2) GC column (5) Auto-sampler vial HP S890 series Il with auto-injector HP 3396C (CBI) 2m vial for auto-sampler, Chromatography-Convenience 400-C80, Tomsic (Tokyo, Japan) <Analytical conditions> (1) Injector temp. 20C (2) Oven temp. 40C (2 min=)> 10 *Cimin 5 170 C (0 min) >30 *C/min (3) Transfer line temp. ~~ > 250C 250C (10 min) (4) Column top pressure 15 psi constant (5) Split flow 50 mmin (6) Injection volume wl (7) Carrer gas. He (99.995) (8) Retention time (Rt, min) Re: 12:2 min (8-2 alcohol) RE 14.4 min (8:2 acrylate) Ri: 5.4 min (8-2 olefin) Ri: 13.0 min (C8 ethyl iodide) RE 7.4 min (C8 iodide) 2:3:5 Results poted andCaslihborwantiionn Fcigu.rv3e.sTfwoor dceutrevremsinfartoimondiofefr8e.n2t raulncso,howlhiicnhAiAncAludbeythsetadnidfaerrdenatddpirteitorneamtemtenhtodrunasr,e prpreeoadkruecaptrroadu(c7i%b)lae.ndSutrhetohgoartiezosnttaanldaaxridsisisnfootrutsheedaminouthnits (seucgt)ioonf,8t-h2eraelfcoorhe,olthfeorvteirfiteidcatloatxhies mislfporolGyCmeFrIiDc. Thre equivalent axis calibration with Fig 3. curves That i, for the 8:2 alcohol calibration sandards horizontal axis of Fig. shows the are shown amount in in Sm Fig 4 for the IPA, which is eaqiubarlaitoonthceuravmeosufnotr cianlimbrlatipoonlystmaenrdiacrdpsrofdorucCt8spurpepcousrisnogrstahraet sthheowrnecionvFeirgys,is5-51,00%. In the same way, provided [The results are armanged in the Attachment 1. in Table 4. All the reproducibility and recovery satisfied the criteria Chemicals Company, ASAHI GLASS CO, LTD. oi70s C2) Fig. 3 Calibration curves of 8-2 alcoholby standard addition method o"no [I JE O qsxmeDntOO ORS iit A | sFES 0LT0 O cl 5oiema on 20 , E[RedtEael GeSE ] Le or Aga TE rE 0 ac w cwmswoowncow ew Fig. 4 Calibration curvesof 82 alcohol calibration standrds. on i os Fy OeDImasi T aE l rR] Sma BL a PTe an I [ELEBE e SaR rcoutem A TLE Je g a | TwET mRY|L Ee RAA a 0p)Rd cn aa 0 Am 20acrwycococsenwaenuwgo5 miL1oAm) roo awe Fig. Calibration curves of 8-2 acrylate calibration standards 9. ChemicalsCompany, ASAHI GLASS CO,LTD, 00705 on 2" os aTseaetthme gZo gacls y=asomo2 aor veoomRo=ue0.s99.9a95m4ount R=099909 om | @Stainmtei | on 0 J D0 wSw2owkotmwcwnc0ngoSrwtuotnoynw vo woo Fig. 6 Calibration curvesof 2 olefin calibration standards J Inte Ty ao | . Ln a heats aii i a2oo [20 0 i EE Sai BE pT p F AiL thik] data a seam Chalo a et wa oA m E aS2m0 aE mHwNnE oePinw5S wH10om) RA mo Fig. 7Calibration curves of -2 hy ide calibration standards L faim T 300m come ff[0ou0F0mn8|a| HHeSRmET BR Tme e aEn me E e o Si Ysoune ee Ta | Som owCoitw coct1u1w5ammeaw mnge we we Fi. Calibration curvesofC8 cide calibration standards Co. ChemicalsCompany, ASAHI GLASS CO, LTD, owns E mMcEe s[esEarRer ,-- -: TT Ta Table 4 Result of determination for 8-2 alcohol and C8 precursors 2-4 WeighBett%orestohleidasnalysis, weig df fo colin ina desiccator hin for gb 15 ots wer rid minis, Abt 1in a0ovpeolseytmaetr12p0rodCufoc wmsorseitghhaned3 0 mines prc, dinetosaiwerighfoirng15bomlienutfese. Tmihxinwgeiwgehli.ngThbisoot lwaswawseipguhteid tanhdotvheensfoloird choonutresn,tansdsficesotcoo, To.2 resultwas(CBI)%. . Chemicals Company, ASAHI GLASS CO, LTD, 040705 2:5 Determinationoftotal inorganicfluoride preament T2otalcoimnboursgtainoicn,Muoisriddee,tetrhmeianmedounbty ouflfilaufoirildieatcoonntaoifnitnhgeinpotlhyempeorliycmeprriocdpurcotduacntdwistuhbosuetquseuncth determination lailation using ion membrane. chromatography. Afer fiation, At 1 first, mi of 2 ml the of the polymeric polymeric product product was (containing fered using practically no polymer) was diluted by 4 ml ofdistilled water and injected nto anion chromatograph conditioned below, 2:5:1 Apparatusand conditions Ultrafiltration Membrane <lon Chromatography> ADVANTEC Ultra Filler Unit USY-1 Ton chromatograph Dionex DX-500 Column Ionpack AGIIHC x2 Mobile phase KOH aq Flow rate 1.0 ml/min Gradient condition time/min 0553 717205 30 Injection volume BmM 2323603605 25 2 S01 Suppressor ASRS Detector `Conductive Detector 2P:5:2roceofddeteurmirnateion potassium Absolute fluoride. calibration curve Fluoride content of of the fluoride was generated for calibration polymeric product was determined using standards consist of this calibration curve by absolute calibration method. 2:5:3 Result Fluoride was determined to (CBI) ppm in AAA. 2 Chemicals Company, ASAHI GLASS CO,, LTD, 00705 2:6 Determinationoftotal organicfluorine (TOF) by determiTnOatFiownabsyoobaninchcrdombaytocgormabpuhsyt.ioAnt-pfyirrsot,hysdbrooultys2i0,mfgluoofridteherpaoplyimnetoriacquperooduusctalwkaalsinhee,aftoeldloowneda hot plate Se at 120 C for 30 introducedinto a combustion-F minutes. Then, known amount ion analyzer conditioned belo, (2-3 mg) of the posymeric product way < Apparatus and conditions > Instrument (cal) Combustion conditions. (cy Ton chromatography conditions Column Mobile phase Injection volume Suppressor Detector Tonpack AGI2+AS12 (Dionex) 2.7mM Na.CO+, 0.3 mM-NaHCO, 10 uL. ASRS `Conductive Detector <Procedure of determination standards (Calibration consist of curve of potassium fflluuoorriiddee. waTsOFgeonferasttaenddaursdinpgolayn(eironaicuhorroomtahtyolgnrea)phy(PTfoFrE,caplirberpaatrieodn ofhineshetoaundsdeeat)redrwmaPisTnFeoEdb.tuasiinnegdtnhedcacloimbpraatrieodn wciutrhvhbeytahbesoroeltuitcalcavlaiblruaetioofn7m6ewtihso,d TanOdFcoofttchteepdolbyymethreicrepcroovdeurcyt <Result> 93:5%. TOF of standard TOF of AAA was PTFE was 71.1%. Therefore the recovery of (CBI and comecte o (CBI usin the the combustion was recovery of standard ealulated to PTFE, TOR canadlutlhetoerdetifcraolmvatlhueescowmeproesiitnigoonodofagthreeepmoelnytmefroricthperpoodulcytme(rtihceoprreotdiuccatl, amount) were (CBI, Empirical 3. Chemicals Company, ASAHI GLASS CO, LTD. 00705 2.7 Calculationoftotal carbon of Atiachm"eTonttal2.caCrabrobnoin ctohnetepnotlyimsecrailccuplratoeddutcoi(sCeBstIi)m,ated by caleultion based on the sample calculation 2-8 Calculationof Molalityof 8-2 alcohol elutionMooflAaiayocfh$e2na3l.coMhoollaliinttyhoef8p-o2lyamlecroihcolpriosdcuacctulssteestditmoat(eCdBb)ymcaallciukl.ation based on he sample 3. References "Purification and 07,2004, contains characterization TSCA CBI of purified Telomes polymer by Asshi Gass, submited to EPA onMay * OECD guidelines 18 (`Determination `Weight Distribution of Polymers using Gel of the Number.Aversge Molecular Permeation Chromatography") Weight and the Molecular * OECD guidelines 119 (`Determination Permeation Chromatography") of Low Molecular Weight Content of a Polymer using Gel * IemuraT,.et.al J. Chromatogr, Vol 1026/1-2, pp 109-116,(2004) Se Chemicals Company, ASAHI GLASS CO, LTD, 040705 Attachment 1 - Validation Example Cme8tphroedcucrasAnorssbieimnepmlpepollvoyamylieedrdatpfirooodnutcmhteest,dheotdermindaeteiiomnionfeCP8FOprAeciunrstohrespaurnidfifeodrptohelydmeetrersmipnraotpioosnedo.f PEqFuOivAalaennt `3StKannodawrnd aadmdoiutnitonomfePtFhOodAt(o5d4e)temreminiseaPdFdeOdA10intthheeppuurirfiifeidedpoploylmyemrerby addon ofp mi of mg/ml oPfFeOpxArp.eepccattrehedadnao(mlhoseuoneluttciioonnnc.tehneAtrpauzrtiiefoinesaddipinoilaolylmn.ersT.ahmeplaemoaunndttowfotshaemapdldeisticoonntsahionuilndgbveariinetdhceornacnegnetroatfion0-s5sthiomoets For exampl, i 1 1g purified polymer. ppm PFOA i expected in the purified polymer, 00, 1.0, 20 ug PFOA is added to the `SoxPhFleOtAextirsacetxitornacbtyedRbOyHRmOaHy a(sMoebOeHavaoirlaEblIeO.H)Faidndailtivoonl,umuelsasfoonriaclaisoanm,pleasndmucsetntbreifiudgeanltisceapl,aration. ceoxnaEtcxittrneadcwtasetodelruRtitOooHnpfrioesrvteihnnettrcobodaumncpdeednbsriaonttaoidoetnnhieonfLgtCih/neMtMShue/ctMHaSPt)LioCanfoceforilnutjmheenc.dtiilIounnttievomonalliufmsneteacanendsdsaarrdMyS.maDsyielnabsteiitoaindvds:eoldveinnttmoathye The PFOA standard addiion calibration curve is plotted area (count) or (PFOA ata (cou/inntetrn)al aginst the added PFOA standard area (count). weight, The vertical axis is 16 the calibration curve (ax + b) satisfied the criteria of B > 0.99, it s valid, & PFOA content obtained fromth calibration curve where PFOA content= ba ory-ntrcept. h. Example calculations are shown below. 15. `Chemicals Company, ASAHI GLASS CO, LTD, 04705 PFOA added to the Ig purified polymer Im 00 10 20 aPnFdOdAilucto"inocnenbtarsaetdioonn a1f0t0er%exrteraccotvieorny emi 000 002 004 PFOA area count 30.300 68.500 113000 noonye | - | = 4135000x04 2250000 R099 samo | ! on ! || ostundat sasion 000000200) PROAGOoNent = O71 ugg = 071 ppm 8Reprisodruecpiebaitleditoynocfetmhoermee(t2hmodesanidn ahlo)moorgsuebsnaeompiflttehseyofputrhiefsiaedmepopluryimfeierd polymer. n1dhehotmwoogePnFeiOtAyovfaltuheespurrcifwieidtiponlhymeer15r%eosfufftihceieenta,vearnadgethveaPluFe,OAbovtahltuee reprodaciiy of th is the average value, method sFRooelucuroovcneosrn)ycnactrraittneireoicnats-eodCfaliPinFborOaLAtiMsoSno.cournvecoornttahienisntga.ndsaolrvdesnt lnk no extracted solution bot pure sandard ihehchoantcenntreatdioninanhgeessthaonudladrdbaedciTqiuhioevnaclmoemenpttohs1oi0dtti(h0oe)n.iotafnedKamOraHd aaadnnddidtwiaaortnershiouhldebseolsveendt sbhyouslidbmeiitngse,n Should no blank. The be detectedo les han calibration curve for the 20% of he standards s count ofthe plotted. lowest range iandard conducted in, concntation for The pro he solve tRehceovcearlyibirsadtieonicunraeve t(hc*exs+lodp)e sfaotistfhiedsthaendcarridteariaionf R)>(h0e.99sliotpies fvoarlitd,he standards. 18 the recovery satisfied the criteria of 70-1209, it i valid Example calculations are shown below, .16. Chemicals Company, ASAHI GLASS CO, LTD. Calibration curve for the standards> PFOA concentration g/ml 000 oor 003 00s oss PFOA area count 10 27000 75,000 130.000 Joo 100000 00 nom i (com) ofe_om0_om . ye 26750000+ | soo Room [-- caflobrratinoncnurdve e | 2R5 eT03sx10 93220 om ape Recovery: 2067500 2574746 = 080 = son Recoivsafefercteyd by bo Recovery doesnot mean the the mati effet and extract value (PFOA determined) ficiency: (amount of PFOA in the puri polymers), but dheeivnailouen(iPFprOacArtieccaolvleyriemdp)os(sPbFeO1A0 aabddaend bietcauthseeplulriPfiOedApoilnymtehresp).rTiheedrpeocloyvemreyrsbmaudstobetximae,s Generally the recovery is determined based on ater definition, te Chemicals Company, ASAHI GLASS CO, LTD. 040705 Example method details "The amount of the purified polymer: 1g Expected PFOA in the purified polymer: 1pm `<Standard addition method> 1 2 000,0.10,0.20 ml of 0.01 mg/ml PFOA 4.80 5.00 ml B(OH is added to the each EXOH solution polymer (total i 5 added 0 the mi EIOH) 1 g purified polymer. 3 PFOA is extracted by ultrasonication and centrifugal separation # 0:1 mi of added and extracted ROH mixed well. is taken out into a vial and 0.9 ml of (EtOH water = 1:1 (wt) is 5 The mixturei introduced into the LOMS(MS). The standard addition calibration vertical axis is PFOA area (count). curv is plotid against (PFOA weight added) / 1g, The Calibration curve for the standards> 7 b0u0t0,pu0r0e1,st0a.n0d5ar,d0s.o0l5utkigon/sm) aPrFe OinAje(ctEeOdHi:nwtoatLeCr/M=S1(:1MS()wt.) solutions (no extracted solution 8 The calibration curve s plotted. On the same graph, standard addition curve is extracted and diluted solution as horizontal axis, plotied by PFOA concentration for the 10 Mthe "recovery"i 100 %, the slopes of two calibration curves are expected to be identical, 18- Chemicals Company, ASAHI GLASS CO, LTD, ours Attachment 2 ~Example Calculationsof Carbon Content [The example is based monomers used in the following on the estimation weight percent that the purified polymer is based on sg. 4 ifeent 60% RICH.OCOCHSCH, (the RE chain distibution average may be equivalent for fy) 35% 25% CHOiCsHH.yO0CCOOCC(HC=HCyH),=CH, 25% HOCH;NHCOC(CHy)=CH, Monomer type Ri(= CyF15)C;H,0COCH=CH, CisHyOCOCH=CH, HHOOCC:HH:,N0HCCOOCC((CCHH;y))=~CCHH,, Formula CI4FIOHT02 C21H4002 C6H1003 cshno2 Mw [g/mol] 5682 3245 130,1 11531 O17n:t7h6a%t b(a2s9i.s6xth0e6C)-cfoontRenItCoHf,suOcChOaCpHo=lCymHeerfwoirlRbteaverage COF19) 27.02% (77.2x0,35) foCr isHy;0COCH=CH, 4 7.47% 1.385% (55.40,025) for L305% (52.250.025)for Sum HOC;H,0COC(CH;)=CH, HOCH,NHCOC(CH,)=CH, Cecontent 1% 206 m2 554 522 The carbon content in such a polymer will be approximately 47.5%. 19. CK hemicals `Company, ASAHI GLASS CO, LTD. 04/07/05 keee e eTmoss moatme a cory ee os Attachment 3 ~ ExampleCalculationsofMolality of 8-2 TB alcohol in polymer ns E a e a 7 [Mesraionto b8. 5t Atapai tpu| gs[og se m g] lig ey ef I f er naa --c Te WiareJt oaedmep] ee me | eegy--|emp] i.