Document O11bq8JJ2kVRwbKvky0Zy0jGw

TRADE SECRET AR226-3163 DuPont-4594 Study Title H-24159: Static, Acute, 96-Hour Screening Test with Pimephales promelas Laboratory Project ID: DuPont-4594 A u t h o r : Robert A. Hoke, Ph.D. S t u d y C o m p l e t e d o n : August 30,2000 P e r f o r m in g L a b o r a t o r y : E.I. du Pont de Nemours and Company Haskell Laboratory for Toxicology and Industrial Medicine Elkton Road, P.O. Box 50 Newark, Delaware 19714-0050 W ork Request Number: J Study C ode N um ber: | ^ ^ I Company Sanitized. Does not contain TS C A CB1 -1 - H-24159: Static, Acute, 96-Hour Screening Test with Pimephales promelas STUDY INFORMATION Substance Tested: Svnonyms/Codes: . H-24159 Haskell Number: 24159 DuPont-4594 Stability: The test substance appeared to be stable under the conditions of the study; no evidence of instability was observed. Sponsor: E. I. du Pont de Nemours and Company Wilmington, Delaware 19898 U.S.A. In-Life Initiated/Completed: August 14, 2000 / August 18, 2000 Company Sanitized. Does not contain TSCA C3I -2 - H-24159: Static, Acute, 96-Hour Screening Test with Pimephales promelas DuPont-4594 SUMMARY The acute toxicity o f H-24159 to the fathead minnow, Pimephales promelas, was determined in an unaerated, 96-hour, static test. The study was conducted with 4 concentrations of H-24159 and a dilution water control at a mean temperature o f 22.1 C (range o f 22.0-22.1C). One test chamber was used per test concentration with 10 test organisms in each chamber. Based on visual observations, the water control and the 1 and 10 mg/L test concentrations were clear and colorless at test start. The 100 mg/L test concentration was clear and colorless with an oily surface film, and the 1000 mg/L test concentration was cloudy with an oily surface film. All water quality parameters were within acceptable limits during the exposure, with the exception o f the pH in the 1000 mg/L test concentration. The pH in the 1000 mg/L test concentration was lower than the pH measurements for the water control and the other test concentrations at test start. Exposure of H-24159 to the water control and nominal H-24159 concentrations of 1, 10, 100, and 1000 mg/L resulted in 0, 0, 0, 0, and 100% mortality, respectively, at the end of 96 hours. No mortality or sublethal effects were seen in the water control test organisms. The highest nominal concentration causing no mortality at test end was 100 mg/L. The lowest nominal concentration causing 100% mortality at test end was 1000 mg/L. Nominal H-24159 concentrations were used for calculation of LCso values. The results are summarized as follows: Nominal concentrations of H-24159, mg/L dilution water control, 1,10, 100, and 1000 96-hour LC50 for H-24159, based on 316 (100 to 1000) nominal concentrations, mg/La H-24159 exhibited low concern for aquatic hazardb in an unaerated, 96-hour, static acute test using the fathead minnow, Pimephales promelas. ` Stephan, C.E. (1997). Methods for calculating an LC50. Aquatic Toxicology and Hazard Evaluation, Proceedings o f the First Annual Symposium on Aquatic Toxicology, ASTM Special Publication 634 (F.L. Mayer and J.L. Hamelink, Eds.), pp 65-84. Philadelphia. b Smrchek, J., Clements, R., Morcock, R., and Rabert, W. (1993). Assessing ecological hazard under TSCA: methods and evaluation of data. Environmental Toxicology and Risk Assessment, ASTM STP 1179. (W.G. Landis, J.S. Hughes, and M.A. Lewis, Eds.), pp 22-39. American Society for Testing and Materials, Philadelphia. Company Sanitized. Does not contain TSC A CB1 -3 - H-24159: Static, Acute, 96-Hour Screening Test with Pimephales promelas DuPont-4594 CERTIFICATION We, the undersigned, declare that this report provides an accurate evaluation o f data obtained from this study. W ork C om pleted by: 1 W ork C om oleted by: Issued by Study D irector: r^&CUAJhjMs^--------- ;--------------- Laurie D . Bouchelle A ssociate Scientist >30 U t U t 30 Eate D aniel J. C. Peterson, B.S. A ssociate Scientist Robert A . Hoke, Ph D. Senior Research Scientist D ate fat D ate Company Sanitized. Does not contain TSC CB1 -4- H-24159: Static, Acute, 96-Hour Screening Test with Pimephales prometas DuPont-4594 TABLE 1 DISSOLVED OXYGEN AND pH OF TEST AND CONTROL SOLUTION SAMPLES Nominal H-24159 Concentration (mg/L) Test Initiation (0 hours) Dissolved Oxygen (mg/L) pH Test Completion (96 hours) Dissolved Oxygen (mg/L) PH_____ Water Control 8.8 7.6 6.8 7.6 1.0 8.7 7.6 6.7 7.6 10 8.7 7.5 7.0 7.6 100 8.7 7.3 5.4 7.3 1000 8.7 5.8 l . T 6.1a a Measurement taken at 24 hours due to total mortality. TABLE 2 MORTALITY OF Pimephales promelas, IN AN UNAERATED, STATIC, ACUTE, 96-HOUR TEST WITH H-24159 __________________ Nominal H-24159 Concentration (mg/L) Water Control 1.0 10 100 1000 Number of Dead Test Organisms / Number of Test Organisms at Study Start 24 Hours 48 Hours 72 Hours 96 Hours 0/10 0/10 0/10 0/10 10/10 0/10 0/10 0/10 0/10 10/10 0/10 0/10 0/10 0/10 10/10 0/10 0/10 0/10 0/10 10/10 Company Sanitized. Does no! contain T S C A C W