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AR 826 _ 0740 ACUTE TOXICITY TO FISH TEST SUBSTANCE Identity: A mixture containing perfluorooctanesulfonate, which may also be referred to as PFOS, FC-95, or as a component of FC-203A. (1Octanesulfonic acid) (CAS # 2795-39-3). Remarks: The 3M production lot number was not provided. The test sample is FC-203A. Current information indicates it is a mixture of 1.34% PFOS, 25%diethyleneglycol butyl ether, 67.85% water, 2.66% Sultone foamer, 3% sodium octyl sulfate, 0.1% sodium lauryl sulfate, and 0.05% tolyltriazole. The following summary applies to a mixture with incompletely METHOD: characterized concentrations ofimpurities. Data `may not accurately reflect the toxicity of the fluorochemical component of the test sample. Method: Not given Type: Acute static GLP: No Year completed: 1975 Species: Pimephales promelas Supplier: Not given. Analytical monitoring: Temperature, pH, and DO Exposure period: 96-hours Statistical methods: LCs values calculated using graphic interpolation. Test fish age: Juvenile Length and weight: Average length = 2 in. Average weight = 1.5 g (wet) Loading: Not given. Pretreatment: None Test conditions: Dilution water: Carbon-filtered St. Paul City water, St. Paul, MN Dilution water chemistry: Not given. Lighting: Not given. Stock and test solution preparation: Not given. Concentrations dosing rate: Once Stabilityofthe test chemical solutions: Not noted Exposure vessels: Not given. Numberofreplicates: 1 Numobfe fisrh per replicate: Not given. Numberof concentrations: five plus a blank control Water chemistry during the study: PH range (0-96 hours): 7.0 -7.0 (control exposure) 000987 6.7- 6.9 (750 mg/L exposure)" `Temperature range (0-96 Dissolved oxygen range (h0o-u9r6s)h:our6s9)-: 70 F 43..7945..86 mmgg//LL ((c7o5n0trmogl/eLxpeoxspuorseu)re)* *be7c5a0usmeg/thLe(hsiegchoensdt hcoingcheensttrcaotnicoenntursaetdiorne)suelxtpeodsiunrteotvaallmuoerstaulsiteydat 72 hours. RESULTS Nominal Element concentrations: values: 96-hour Bk control, LC50 = 300 m1g0/0L,.250, 500, 750, and 1000 mg/L Element values based on nominal concentrations Remarks: Substance Testing was conducted on Remarks field. The values the mixture as described in the Test reported applytothat mixture and not the fluorochemical proportion alone. CONCLUSIONS The test sample 96-hour LCso for fathead minnow was determined to be 300 mg/L. Submitter: 3M Paul, Minnesota, C5o5m1p3a3ny, Environmental Laboratory, P.O. Box 33331, St. DATA QUALITY TRehleiraebilisitay:lacKkiiomfischcahrraactnekriinzgat3.ionToefsttihneg lacks record test sample. of methodology used Test solutions were not aflnuaolryozcehdemfiocrtaelsptrsopuobrsttiaonncien concentrations the solution. and confirmationofthe amount of REFERENCES STth.isPasutlu,dMyNw,asLacbonRdeuqcuteesdtbnyutmhbee3rM25C6o3m,p1a9n7y5,. Environmental Laboratory, OTHER Last changed: 6/26/00 7988 es Le EF 740101 2 mii av OrgA =>)Repsyaa ENVIRONMENTAL ENGINEERINRG ew pboa br 2 SLR 253 AQUATIC TOXICITY MORK SHEET - ] tems Site, cage . Type Test, 96 Hours - i Material Tested Test Organism__ FatheadMinnow, Avg. Weight 23 Size "2 che Toate stared 17-75 Date Completed [-)1-75 2 Time Started, 2:00pM Plotted LCgy 300 PPR Dilution Nater_Carbon Filtered - St. Pau City mater BEST COPY AVAILABLL 2h 48 es 72 hs 56 his J eal yy Ilse] Conc. Survi`val pH D.ppm0 100 [20]5s] 0Tz 50] joo[7da] Survisval pH D.ppm0 Surv`i+val pH 0pm.0 Survi+val pi P0I.0 peor] loo[695]a] Joo as |90 lata] 60 00 JroilopaJrnola|s] to lesgap nleal]as] & soi 2| % l3lap] en Jeolas] sa20s[sr] las| Jo fel ze le] alo eq34] yan | mled47] 20 [esas] lqoedlao] 0 MergeTempeiature - 6 _7e. 2D 0= } Ta Ri ER ool Cal tag cateutetton BEST COPY AVAILABLE PSE TFET eT aa he FRO ee Ei EHREE FAR Hee 5 i TN Peo EL eFTl H FEEL De E 53 Br SER 3 gemE ER Ce I ET T TE g r Halalo ocn l a i nE ns n IINeEL HERH T EE BESE T SEFE ES RT BUR aH Seth HETE . HE TEE : REI ( I HFONE ! : ; 5PaoditiacgkaBn egcn Sm neg 7%INGW]n 000989 igh -- ms RRR ey