Document NeY3y0nVrq0yDJ9VmpEM2jokV

1' 2-41 E . J. d u P o n t d e N e m o u r s & C o m p a n y INCORPORATI W il m in g t o n , D e l a w a r e 19898 P O L Y M E R P R O D U C T S DL ''RTMf NT EXPEPIYCNT ``i. PERSONAL AND CONFIDENTIAL OC: A. J. Dahl B. W. Karrh L . K . Fa pa Pral File I .C. - 353 - N11400 - 2b9 s4/226r !Sti July 16, 1981 TO: DR. A. BINNERTS - PPD, Dordrecht FROM: S. S. STAFFORD ANALYSIS OF BLOOD SAMPLES FOR PERFLUOROOCTANOATE (Job No. 810-578,8L0-699; PRAL Nos. 81-2239-2243,81-2393-2397,81-2530-2534, __________________ 81-2613-2618; Notebook Nos. E22514,E26238__________________ As requested in your letters of 5/20, 5/26, 6/2, and 6/10/81 to L. J. Papa, the 21 blood samples submitted then have been analyzed for perfluorooctanoate (Cg). Results and sample identification are given in the attached table. As noted there, the analyses were done using a gas chromatographic method specific for Cg (Lab Method Number ES-567) but results have been reported as ppm F for comparison with total organic fluorine analyses. Precision is + 102 relative standard deviation over most of the concentration range, somewhat less at the lowest values. The lower limit for quantitation is 0.007 ppm F (0.01 ppm perfluorooctanoic acid), with a detection limit of A/ 0,004 ppm which can be dis tinguished from the reagent background but not well quantitated. Please contact me (772-4440) or L. J. Papa (772-2745) if you have any questions regarding the analyses. General questions on blood sampling can be directed to J. V. Raines or L. F. Percival. Attachment jah Key Words: Perfluorooctanoic Acid Perfluorooc tanoate Blood Analysis GC 1 ` a a odei o( Itnfiqs we re doing something about 000106 EXP000033 EID713847 TABLE I CON CENTRATION OF PERFLUOROOCTANOATE IN BLOOD (a) Sample PRAL No. 81-2239 81-2240 81-2241 81-2242 81-2243 81-2393 81-2394 81-2395 Date Sampled 5/19/81 5/19/81 5/19/81 5/19/31 5/19/81 5/26/81 5/26/81 5/26/81 P.R.No 04392 03240 03789 04822 03370 03824 04841 01999 81-2396 81-2397 81-2530 5/26/81 5/26/81 6/2/81 037 26 02978 04793 81-2531 81-2532 81-2533 81-2534 81-2613 81-2614 81-2615 81-2616 81-2617 81-2618 6/2/81 6/2/81 6/2/81 6/2/81 6/9/81 6/9/81 6/9/81 6/9/81 6/9/81 6/9/81 04108 04499 03859 01611 01530 01951 03293 02180 04502 02141 Name GC Analysis Date Analyzed 6/4/81 6/4/81 6/8/81 6/3/81 6/8/81 6/10/81 6/10/81 6/10/81 & 6/16/81 6/10/81 6/15/81 6/9/81 6 6/16/81 6/9/81 6/9/81 6/9/81 6/9/81 6/16/81 6/16/81 6/16/81 6/16/81 6/16/81 6/16/81 I [Cr ], UR FIr blc 1.4 1 .4 8.1 3.4 4.5 7.8 5.9 6.6 6.6 2.6 20. 6.5 3.9 9.2 4.9 4.8 0.59 3.6 4.1 7.7 2.1 (a) Analysis as described in Lab Method ES-567 ("Determination of Perfluorooctanoic Acid in Blood, Gas Chromatographic Method", S. Stafford, 4/3/81), using the packed column GC analysis with perfluoro-n-octanoic acid as calibration standard. (b) Although the analysis is specifically for perfluorooctanoate (acid or salts), concentrations are given in ppm fluorine for comparison with the results of total organic fluorine analyses. (ppm F = 0.688 x ppm perfluorooctanoic acid) Estimated uncertainty is + 10% relative standard deviation. The lower limit for quantitation is 0.007 ugF'g. The detection limit is 'w 0.004 UgF/g, but concentrations in that range cannot be well quantitated and are reported as < 0.007. None detected (n.d.) is reported for samples with [Cg] 0.004 ppm, which cannot be distinguished from reagent background. REDACTED* 000107 EXP000034 EID713848 il !* E. I. d u Po n t de N e m o u r s & C o m p a n y INC.CJN*i>N<> TI O W il m in g t o n . D e l a w a r e 19898 P O L Y W I R P H O D U C ' !r DL * ' N T K*F NH EI X P C R I K ' f N l A L S I A I I C ' N PERSONAL AK3 CONFIDENTIAL cc: A. J. Dahl B. W. Karrh L. J. Papa Pral File l.C. - 353 - M 1400 - 269 *AZlCrl$10 July 20, 1981 TO: DR. Y. L. POWER - PPD, Parkersburg FROM: S. S. STAFFORD iA A ^ / ANALYSIS OF BLOOD SAMPLES FOR PERFLUOROOCTANOATE (Job No. 810-578; PRAL Nos. 81-2952-2955; Notebook Nos. E225I4,E26238) As requested in your letter of 7/14/81 to L. J. Papa, the 9 blood samples submitted then have been analyzed for perfluorooctanoate (Cg) . Results and sample identification are given in the attached table. As noted there, the analyses were done using a gas chromatographic method specific for Cg (Lab Method Number ES-567) but results have been reported as ppm F for comparison with total organic fluorine analyses. Precision is + 10% relative standard deviation over most of the concentration range, somewhat less at the lowest values. The lower limit for quantitation is 0.007 ppm F (0.01 ppm perfluorooctanoic acid), with a detection limit of n, 0.004 ppm which can be distinguished from the reagent background but not well quantitated. Please contact me (772-4440) or L. J. Papa (772-2745) if you have any questions regarding the analyses. General questions on blood sampling can be directed to J. W. Raines or L. F. Pere iva 1, Attachment j ah Key Words: Perflurooctanoic Acid Perfluorooctanoate Blood Analysis GC T h o u s E w o rld o ! th in g s wc- re- d o in g s o m e th in g a b o u t 000108 EXP000042 EID713856 fr--** -- -- ___ TABLE I CONCH NTPJyT T O N .0_F_PE! Sanrle FRAL Mo. Date Sampled 81-2952 6/9/81 81-2953 6/12/S1 81-2954 6/18/81 81-2955 6/18/81 P.R.No. 4543 WS U'S 1581 CC Analysis___ Dace Analys' d Ica'o.T-I 7/14/81 0.61 7/14/81 7/14/81 0.51 1.2 7/14/81 0.15 (h; / (a) Analysis as described in Lab Method ES-567 ("Determination of Perfluorooctanoic Acid in Blood, Gas Chromatographic Method", S. Stafford, 4/3/81), using the packed column GC analysis with perfluoro-n-octanoic acid as calibration standard. (b) Although the analysis is specifically for perfluorooctanoate (acid or salts), concentrations are given in ppm fluorine for comparison with the results of total organic fluorine analyses. (ppm F = 0.6S8 x ppm perfluorooctanoic acid) Estimated uncertainty is + 1G% relative standard deviation. The lower limit for quantitation is 0.007 UgF/g- The detection limit is ^ 0.004 ygF/g, but concentrations in that range cannot be well quantitated and are reported as < 0.007. Mone detected (n.d.) is reported for samples with [Cg] < 0,004 ppm. which canr.ot be distinguished from reagent background. *REDACTED* EXP000043 EID713857 000109