Document NeQrN4Drn76LR3jexjmn97ERQ

AR 426 -- 0834 ACUTE TOXICITY TO FISH (FATHEAD MINNOW) IESTSuBsTANCE Identity: AbemriexfteurrreedcotnotaaisnPinFgOSpe,rfFlCu-o9r5oo,cotranaessualfcoonmapteo,newnhitcohfmFaC-y60a0l.so (he1p-tOacdteacnaefsluulofroon-i,c pacoitda,s1s,i1u,m2,s2a.l3t,,3C,4A,S4.5#.52,769.56-.73.97-,38).8.8- RFCe-m6a0r0ks(:BelgiTuhme).3MCuprrroednutcitnifoonrmloattinounmibnedriwcaatses 2367. it is a The test sample mixture of 1.0% is PFOS, 81.20% water, 12.00% diethylene glycol butyl ether, 1.00% sodium o0c.t8yl5%sulxfaantte,ha2n.g0u0m%,p0ro.p1a%nNe-(S3u-lctholnoreaaflolaymle)rh,e1x.a0m0i%nisuomdichulmordiedcey,l0s.u8lf0a%te, starch, and 0.05 % tolyltriazole. Tchhaerafcotlelroiwziendg scounmcmenatrryataipopnloifesitmopuarimtiixetsu.reDawtiathmianyconmoptleatcceulryately sreafmlpelcet.the toxicityof the fluorochemical component of the test METHOD: Method: Not noted. Test type: Acute static (Range-finding) GLP: No Year Completed: 1983 Species: Pimephales promelas Supplier: Commercial supplier in Brady, Nebraska Analytical monitoring: Temperature, pH, DO Exposure period: 96-hours Statistical methods: Not applicable. No mortality at highest cToensctenftirshataigoen:teJsutveedn.ile Average Length and weight: Length = 3.6 cm = Weight = 0.39 grams Loading: 0.65 glL Pretreatment: Not noted. Test conditions: Dilution water: Carbon-filtered well water, St. Paul, MN. Dilution water chemistry: pH: Dissolved Oxygen: Temperature range: 82 9.6 mg/L 18C Lighting: Not noted 002028 Stock and test solution preparation: Test solutions were created by direct weights additions. CStoanbcielnittyraotfitohnestdesotsichnegmriactae:l Once solution: Not noted Exposure vessels: Wide-mouth one gallon glass jars containing 3 liters of test solution. Number of replicates: One Number of Numberof fish per replicate: 5 concentrations: Four plus two blank controls Water cphHemriasntgrey(0du-ri9n6ghtohuerss)t:udy: 87..70-88..22 ((c1o,n0t0r0olmegxlpLoseuxrpeoss)ure) Dissol5ve.d89O.xy6gmegnlLra(ncognetr(o0l --ex9p6oshuoruerss)).: Temper1a.t7u9re.6ramnggleL((01-,09060 hmogu/rLs)e:xposure) 18-21C ReSWts Nominal Element vcaolnucee:ntrat9i6o-nhso:ur2LbClsaonk= c>o1n,t0ro0l0s,mg1,/L10, 100, and 1,000 mg/L Element value based on nominal concentrations. RSuebmsatraknsc:e TReesmtianrgkwsafsielcdo.nTduhcetveadluoen rtehpeomrtiexdtuarpepalisesdetsoctrhiabtedmiixnttuhree Taensdt not the fluorochermical proportion alone. conclusions The FC-600 86-hour LCsq for Pimephales promelas was determined to be >1,000 mg/L. Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 pataQuaTY tReesltiianbgi.litHyo:weKvieirm,istchhe rsaanmkipnlge2p.urTithyewsatsudnyotmeperotpseralllytchheacrraictteeriraizfoerd qaunaldity the study lacks analytical confirmation of the amountoffluorochemical proportion in the solutions. 002023 REFERENCES This study was conducted by 3M Company, Environmental Laboratory, St. Paul, MN, Lab Request number 8875, 1983. oTHER Last changed: 6/28/00 002030 Ly BEST COPY AVAILABLE TTRAENT ASIAN vo ese ZitzWh EXPLORATORY Eri NG TEST) LR a 5 meesi reves L224 (ego25] Test Oresnisn ote)auulsoueeo Fpl,A! ute Recidyplisfrzsarenvo.LBS Maw 0,5) grams Ave. SiH No. Organisms per dose 5 Test SolutionVolume3Liters/All-Glass Tank Tank Dimensions:WIDE-HOUTH 1 GALLON GLASS JAR Tine Started_4/HOfH{vate started, 2 fe fas Date Completed__Z.Lo/65 `Dilution Water_Carbon-filtered well water (Temp. /83 C.. 0.0._Z/p pon. EZ teInvestigator. UE FS "igo (95% : CoEifAee Limits),--io00ugl] AT. otal Wows Hours Hours 6 Hour uo eme1 r [==: Added 0 Talal No. Dead|p ppm 0No.De Jad arles [pH | pp l] 0kib3l No.Dead bi pps ]| No, 0 tdso DeadbHlppm HIteUsal ==--105 BBilrl 00JamLalAOthialinill 0o tbdees Alo VON eUCen |[70 b7a27pa| 0 2A1T2l0i01l| 0 Y2a,7ad|W0o 7 fdas Aea [meal 0rBrA 0 aya 0rhiezralo1A rrr me || 77 : vee E752/7S N2 || 20 07 | 2/4 N52 RBH 0[7B Solu fp Comments:XIAN UOEIGHTS, (USE cllllctyOLR MIU ettoets ctr ton voreatiey o Trane {|| mptoms or nections || ete, apieTotal No, MEE Affected eel|| | J7e721[||WJ. a7e2[A7bAe ||WAfia ie | RT CL r Ie [Arlaee W WeeWAhe : DEETC | Seon, T | 7/71 |e ZTEr2 a s |W 1, em