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AR 226 -- 0798 ACUTE TOXICITY TO AQUATIC INVERTEBRATES (DAPHNIA MAGNA) TEST SUBSTANCE \dentity: ArefmeirxrteudretocaonstPaiFnOiSng, PFeCr-f9l5u,oroorocatsanaescuolmfpoonanteen,twohficFhC-m2a06yAa.lso(1b-e Octanesulfonic acid) (CAS # 2795-39-3). Remarks: is FC-206A, aTnhaem3beMrplrioqduiudc.tiCounrlroetnntuimnbfeorrmwataisoncion7d8i5c-a2t2e.s it The is a test sample mixture of S0u.l9t2o%nePfFoOaSm,er1,81..7755%%dsieotdhiyulmenoectgyllycsoullfbauttey,l1e.th2e%r,po7l5o.x4y4et%hwyalteenre, 1.83% `monooctylphenyl benzotriazole. ether, 0.06% sodium lauryl sulfate, . and 0.05% cThhaerafcotlelorwiiznegdscounmcmeantrryataipopnlsieosftiompaurmiitxiteusr.e Dwaittha imnacyomnpoltetaeclcyurately reflect the toxicity of the fluorochemical componentof the test sample. METHOD: TMyepteh:odA:cuNtoetsgtiavteinc. GLP: No Year completed: 1979 SSuppepcliieesr:: DIanp-hhnoiusaemcaulgtnurae. Initially from OSH Wis. Analytical monitoring: Temperature, pH, and dissolved oxygen. TEexsptosourrgeanpiesrmioadg:e:48-Nhootugrisven TSteasttisctoincdalitmieotnhs:od: Probit analysis Dilution Dilution water: water Carbon-fitered chemistry: well water Hardness: Alkalinity: 236 221 mg/L mg/L as as CaCOs CaCOs Conductivity: 430 pmhos/icm SLitgohctkinagn:dNtoetstgsiovlenution preparation: The test material was added tEoxtphoesduirluetivoenswsaetlesr: on a weight / vessels with volume 100 mL basis. exposure solution NNuummbbeerr ooff dreapplhincaitdess:pe2r replicate: 20 Numberof concentrations: five plus a blank control directly Water cphHemriasntgrey (d0u4r8inhgoutrhse):study: 001616 87..60-- 88..00 ((c1o8n0tr0olmge/xLpoesxuproes)ure) Temperature range (0-48 hours): 19-20 C Dissol6ve.d98ox.y5gmegn/rLa(ncgonetr(o0l-4e8xphoosuurrse)): 4.985 mg/L (1800 mg/L exposure) RESULTS mall. Nominal concentrations: Blank control, 560, 750, 1000, 1350, and 1800 Element values: 48-hour ECso = >1,800 mg/L (Replicate 1) 48-hour ECs= >1,800 mg/L (Replicate 2) Element values based on nominal concentrations. Remarks: `Substance TReesmtianrgkwsafiselcd.onTduhcetveadlouensarmeipxotrutreed as described apply to that in the Test mixture and not the fluorochemical proportion alone. CONCLUSIONS The test sample 48-hour LCso was >1,800 mg/L, the highest concentration tested. The combined percent mortality for the replicates at this concentration was 27.5. Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY RTehleirabeilisitay:laKckiiomfischcahrracatnekriinzgat3.ionTeosfttihneg tleasctkssarmepcolredaonfdmtehtehostduodlyoglyacukssed analytical confirmationof the amountoffluorochemical components in the solution. REFERENCES TSth.isPasutlu,dyMNw,asLacbonRdeuqcuteesdtbnyutmhbee3rM49C7o0m8p,an19y7,9.Environmental Laboratory, OTHER Last changed: 6/26/00. 001617 : ENVIRONMENTAL LABORATORY LR 4470S Ce AQUATIC TOXICITY DATA SHEET ort = Lz Type Test_4Q-W ve Material Tested YC-206 A oy 7 Test Organion Do Pit, TM Utirca__ OSH, Lis, deco Rect, 4-119 Baceh No._ Avg. we grass Avg. Size Of No. Organisms per dose ze Tost Solution Volume!O3i%ka/All-Glass Tank Tank Dimensions: -3xdi--ca-scnadd-e2ebp Time Started [06pF go, Date Started D=/2-29 Date Completed 2-14-19 Dilution Water_Carbon-filtered.well nen (Towp._20_C.,, 0.0._&.C oe,24 5.0 ` J(mFeulsltSiiggnaattourree S\nacetivuy TT oo Hows Bigg (55% Confidence Limits) 293 %0 Hours Fo FCF 5 Wow SI TE eea eel ra Yo, Added|| No. Dead| ph | pa'f| No.Dead |pH [ppl No.Deabdi{ pm |INo.D [emer [UT] 0 Jeena] 0 JeoleaN TT [1] [cee [cc 11 Teelnoll Tagle=l] NJ1 TT] oe [oe OTT1 oI1 TTNT] [ooo] 1eelloT[ 11 o[ T MT "TINH TT] [resol] vec] +TT TT1 T T IT TLZIN --T] T_ligeo I igoll 3 heleall 6 Taslgall AT TT N11 r Tr f TT ZT (fr rr TT 7TTT] me [awe we J] site goats||50| 70 9-14-9 13|| Comments: I obliza bie Effects other than Mortality EC, conTce. Total No, Affected mg/1" || symptoms or Reactions|| 24-tr. 48 br. 72-Hr. 9-Hr. emer]I] rr nrnM [fr fn J; fr 7 [nr @-- 71 1 rm Signature & Date T Comments: ere AA 1 ASTE 001R 618 ENVIRONMENTAL LABORATORY 4910s . AQUATIC TOXICITY DATA SHEET Remeron Type Test_4@N Pouve Sane atorial Tested_YCo206 4 fae 135% Test OrganisnDaPwia "NS Source OSM ic. Dato Rec'd._4-) 28atch No_. Ava. We grams Avg. Size__ OM No. Organisms por dose 20 Test Solution Volume'SPANGN/A11-Glass Tank Tank Dimensions: 3a6xn21dc-abomdecp Tivo Started \O. Zo uate Started__7-)2-7F Date Comploted___2- [4 77 DInvestilution Water_C3arbon-filtered well wat8Cegr v((T9e5m%p.Con2fid0enceC.,LimiD.0._- ppTmo, ipsH .6 opine wo] on Une21 ges -- TTT] Tew! iy 8 Hours Wow 5%Tow EE EmEmEw No. | mg/1 [emer [[ ZAPdedFed || II N o. Dead| pH| pp 0 Jeslos]l 0JeolazN_[][1] No.Dead| pH |ppm | No.Dead pit[ ppm {[No.Dead [see [l4 [I 0Tse[zoll o[olga NTT II A [2cell ocfl 0 [1ToToelsall NT71 [ioooll roof 6 T 1 11 0 Toalcel TIS 1 Cd eco TT alee TL IN [rtodl soll 2Twice &Pals PII~] rn TT Tm T N pene rT L termT te ||r ye Me T Z NM e rfT] Comers: Lonmasibizasion pe OPY AVAILABLE Effects other than Mortality Ec CToAncT.E Total No. Affected g/t" || Symptons or Reactions|| 24-tir. 48 tir. 72.Hr. ome][7] rn 1 [rf rrrr rr Signature & Date Comments: ee 96-Hr. - ee vg lw 001619 Horc--. pAT 0 TERErrr fos i TENE HE TEE A:rosie ol fe Pprrt ae :EZRETRsen in ee na E en ekeE ceoee reanrE e vom2& {EprR amEtDitan [iE aa rma L:emamd onCse g2aSo g Bide mawmw 38 E CpBsRiE $5es IS ;When eo TR sa RSS r-- i-- ra ngdh maYaemaarera,r po 2> |BF ee e atone 33 ' Ce SVIAUNINE IFSNS elesatari) . AR REQ NO i NDING) a EE J pu - eur wor ATTACIBENT #1 SAMPLE DESCRIPTION : 3 Phen FILTERED WELL 3 = RIQA A RpoM . . 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