Document NeMR0xzE4vR7kQ9XxVrGbJ2Nw

ACUTE TOXICITY TO FISH (FATHEAD MINNOW) TEST SUBSTANCE Identity:A mixturecontainingperfluorooctanesulfonatweh,ich may alsobe referredtoas PFOS, FC-95, or as a component ofL-4647. (1Octanesulfonicacid)(CAS # 2795-39-3). Remarks: The 3M productionlotnumber isgivenas both NB# 52393-334, and cc803-17. The testsample is1.356% solutionofL-4647. Current informationindicatesitisa mixtureof6.6% PFOS, 20% polyethyleneglycol, 20% hexahydricalcohol,39% sodium decylsulfate1,3.2% Hydroxy foamer, 1% sodium ethylhexylsulfate,0.1% methylhydroquinone, and 0.1% biphenylols,odium salt,tetrahydrate. The followingsummary applies to a mixture with incompletely charactedzed concentradons of impudties. Data may not accurately reflectthe toxicityof the fluorochemical component of the testsample. METHOD: Method: Not given. Type: Acute statidcefinifivaend range-finding GLP: No Year completed: 1960 Species: Pimephalespromelas Supplier:Commerical supplierB,rady,NE. Analyticalmonitoring: Temperature,pH, and dissolvedoxygen. Exposure period: 96-hours Statisticamlethods: The LC50 valueswere determinedby themoving average angle method. Test fishage: Juveniles Length and weight: Average length 32 -36 mm Average weight 0.37- 0.40g (wet) Loading: 0.46- 0.75g/L Pretreabnent:None Test conditions: Dilutionwater: Carbon-filterweedllwater Dilutionwater chemistry: Not given. Lighting:Not given. Stock and testsolutionpreparation:The testmateriawlas added directly tothe dilutiownater on a weighttvolumebasisforthe range-findinagnd the firsdtefinitivtest.The testmaterialwas added from a stocksolutionb,ased on the activeingredien(tCartridgAegent),whereineach mL added was equivalentto 10.356 mg CartridgeAgent forthe second definifivtest. Concentrations dosing rate: Once. Stabilityof the testchemical solutions: Not noted. Exposurevessels:Wide-mouth1 gallognlassjarscontaini3ngL test solutionforthe range-findingtest.All-glasstanks (35x2Ox25) containing16 L testsolutionforthe definibvetests. Number of replicates: 1 - 2,depending on the test Number of fishper replicate:5 - 30, depending on the test Number of concentrations: fiveplus a blank controlforthe range-finding and the second definifivteest.One plus a blank controlforthe firsdtefinibve test. Water chemistry during the firstdefinitivestudy (5/6-10180): pH range (0-96 hours): 7.5 - 7.8 (controlexposure) 7.4- 7.8(1550 mg/L exposure) Temperature range (0-96hours): 17 - 18 OC Dissolved oxygen range (0-96hours): 3.8- 10.8 nig/L(controelxposure) 1.4- 10.8 mg/L (1550 mg/L exposure) Water chemistry during the second definitivestudy (6124-28180): pH range (0-96 hours): 7.9 - 8.0 (controlexposure) 7.5- 8.0(103 mg/L exposure)* Temperature range (0-96hours): 17 - 19 *C Dissolved oxygen range (0-96hours): 4.8- 9.1nig/L(controlexposure) 1.4- 9.1nig/L(103 mg/L exposure)* 103 mg/L (intermediateconcentration)exposure values used because the highesttwo concentrationsused resultedintotalmortality by 24 hours. RESULTS Nominal concentrations: Blank controland 1550 mg/L forthe firsdtefinifive study (conducted 5/6-10/80).Blank control,10.4,58, 103, 248, and 580 mg/L forthe second definibvestudy (conducted 6/24-28/80). Element values: 96-hour LC5o = >1 550 mg/L (firsdtefinifivsetudy) 96-hour LC50 = 154 (126 - 188) mg/L (second definitive study) Element valuesbased on nominalconcentrations Remarks: Testingwas conducted on a mixtureas describedintheTest Substance Remarks field.The values reportedapply to thatmixtureand not the fluorochemicalproportionalone. CONCLUSIONS InconsistLeCn5t0resulwtesreobtainbeedtweetnhefirasntdsecond definitisvteudies.Inthefirsdtefinitisvteudy,whichevaluatedone exposure concentratio(n1550rrwj/Ln)o,mortalitwyas observedduringthestudy.This studyresultisconsistenwtiththeresultosftherange-findinsgtudy,inwhich no mortalitwyas observedinany exposureconcentratio(nhighesctonc.1500 mg/L).Inthesecond definitisvteudy,whichevaluateda seriesofexposure concentrationfsrom 10.4to580 mg/L,totalmortalitwyas seen atthehighest two concentration(s248and 480 mg/L),and an LC5oof154 mg/L was obtained.Based on theresultfsromtherange-findisntgudyand thefirst definitisvteudy,theresultosbtainedduringthesecond definitisvteudyare questionableT.he firssttudywas based on thetotaslolutionw,hilethe second was based on thecartridgaegent. Submitter: 3M Company, EnvironmentalLaboratoryP,.O. Box 33331,St. Paul,Minnesota,55133 DATA QUALITY ReliabilityK:limischranking= 3. Inconsistensttudyresultwsere obtainedin thetwo definitisvteudiesconductedwiththistestsubstance.The studies alsolackinformatioonn puritoyfthetestsubstanceand actual measurements oftheamount offluorochemicaplroportioinnsolution. REFERENCES These studieswere conductedby the3M Company, Environmental LaboratoryS,t.Paul,MN, Lab Requestnumber 5623S, 1980. OTHER Last changed: 6/26/00 Type Test Test Organis ENVIRONMENTAL LABORA'rf)RY AOOATIC MXICITY DATA ST[FET EXPLORATORY (RANGE-FINDING 7E T) 0 LR - vel&l;v-OlIfi-iIOC-k/ torial Tes.-d I Date keg-! LoLt?e- Avg. Wt. gram Avg. Sizklic-CN No. Organisms per dose Test Solution Volume 3 Liters/All-Glass Tank Tank Dimensions:WIDE-MOUTH Time Started Date Started Dilution Water Carbon-filtered moil-m&ter (Temp Date Completed 0 C.; 0.0. 5 I GALLON GLASS JAR ppm, PH Investigat (FulSl@Siiippat@tat 95% Confidence Limits) 0 -init. Tm Conc Total Asoun t Total Ho urs D.. 48 Hours 72 -HLoH P.O. Total D.O. Total 96 Hours tal Added NO. Dead oH ppon-II No. Dead PH IVON No.Dead I IPPU @moo De ad t;oa .Control C4 100 1500 0/5 7.-7 Cl,'S-_ -7 L 0/57 0/5 -7,17-,1-/ 40/E Z-1 -Z-0. -I7- It9 10/,4@ILI L-9- Test Te!N. *C.-.. Comments. 7,C)-eat@ Lieu LD. @.5ow" 161 L-L)&-EM i:ffectsotlierthan Mortality A.A A MglemT EC so e)tiZEE Tan k t No. Sy!ptoms or Reactions Cont'rol 24-Hr. Total No. Affected 48 Hr. 72-Hr. 96-Hr. L Type Test Avg. ENVIROTINIENTAL LABORATORY AQUATIC TOXICITY DATA SHEET L Testi;h@ L DEFINITIVE (FULL-!SCALE TEST),-., I. Material Tested er ate Rec iFg eWrI Me, LLk%rO- -0 LI;@8B,aDtch No4f:i% OrganismEOW&LmAaf"source ose,!S_0_ Loading FactoqQ,7_6mg Wt.-Q.-4-grams Avg. Size.Zi._cImo No. Organisms per d Tank Dimensions: 35x2O cm and 2'5.4q"deep Test Solution Volumel.(oLiters/All-GlassTank Time Started Dilution Wate Date Started -4@@ &U water (Temp. Date Completed__. 0 D.0. 10. 8__Hm, pH InvestigatO (Full Signat rank Md. n Cone. mall Total Amount -Added_ c (95% Confidence Limits) 50 Nut.-- 2 Hours Total D.O. No. Dead _pH ppm 48 Hours ours 0 Total D.O. Total .0. Total N Dead H m No.D ead H m No H pm Control < rir 0 Test Temp. C. fnLtLai4t Comments: Date I 'IRUO, Effects other than Mortality Tannok@l H NN o. c@l.Co@ntrol /1-rrri S m toms Or Reactions Ile 24-Hr EC so- Total NO. Affected 48 Hr. 72-H 96-Hr. @',lmments: ENVIRONMENTAL LABORATORY AQUATIC TOXICITY DATA SHEET DEFINITIVE (PULL-SCALE TEST) LR -,;/a2 Test')&-IAPfC,,,V-e Material Tested Lot .'es.Otrgani sm TCN\.,e-k4 V,^,,-,-,.-4-soux-ec lp,y-r, @,,Date Pec Id r3 5-,g DBatch kvg. Wt. grams Avg. Size__2_,-L an No. Organi--= per dose '-)0- Ioading Factor mg/l 7ish Disease Treatment: rest Solution Volume Liters/All-Glass Tank Tank Dimensions: 35x2O an and 25 an deep Cime Started Date Started 41- fsD Date Cm@eted 6-2-8-80 Dilution water-Carbon-filtered well water (Tenp. C. , D.0. ppin, pH___Z Envestigator\f-v, (Full Signature) cf k-,d (4(@95 , rnn ide=e Lbuits _@jeo,@er t ft,- Lc 5-t= 15q,2- 0 @L-4-f Tank No. Initial Total Conc. Amcxmt rng/l A AWed "\P@N Contro]IK 24 Hours 48 Hours Total No. pH D.O. Total No. pH Dead ppu I Dead 0q 72 Hours 96 Hours D.O. Thtal No. PHID.O. Total No. pliD.O. pM Dead ppn Dead ppn k. .114.9 C) -7, 4.g Co "yyoik 1 0 @.7 0 0 ?.q 6. 0 -7.9 89. b 0 -3:9 (1 0 7.95-2 0 7.9 4. 0 -7,1 0 717.9 0 2-.S &o 0 '7.6 2-419 3 gel ao @2 C) 5to 0 ?; 1:@-L) 1.4 ;2-0 0 Test Tegy. OC Initials & Date ccmrients: Cl% C- v@,.o pt Ck c (11 VL4 C@ c 'v-b rArY.Y.-@arp YW $econdary Effects (otherthan Mortality): BC50 Tank No: Symptans or Reacticns 24-Hr. 48-Hr. W Affected 72-Hr. Control MCI @, 0- ri e 0 --c lei C, c 96-Hr.