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TOXICITY TO AQUATIC INVERTABRATES (E.G., DAPHNIDS) TEST SUBSTANCE Identity: N-Ethylperfluorooctanesulfonamide; may also be referred to as U1464, EFOSA, EPPOSA, F-6309, or FX-12. (1Octanesulfonamide, 1,1,2.2,3,3.4,4,5,5.6,6,7.7,88,8heptadecafluoro-N-ethyl-, CAS # 4151-50-2) Remarks: Material is a white solid, Lot 505 (cc842-3, F630, L-1089). Sample purity was not recorded. `The following summary is abbreviated due to the fact that this study has been superceded by a more recent test. METHOD Method: Not provided TGyLpPe:: NAocute static Year completed: 1984 Species: Daphnia magna RESULTS 48-hour ECs (immobilization): 3.2 (2.9-3.6) mg/L (Probit analysis) DATA QUALITY Reliability: Kiimisch ranking = 3. This study was tested in excess of solubility and actual concentrations were not measured. Results were abdaesqeudatoenlynocmhianraacltceorniczeendt.rations. Additionally, sample purity was not REFERENCES `This study was conducted by the 3M Company, Environmental Laboratory, 1984. OTHER Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 Last changed: 5/18/00 006072 CHEMICAL EENNVVIIRROONNMNEENNTTALALLEAFBFOERCATTSOERYVALUATION by,aPy,Ai T TOXICITY TEST: SUMMARYREPORT dg Chemical eres gpmSp frst) rest yee:De(u bus<Ss er.c. sos 1cs0/chss15: R ER pwn ante 40)/5.2.(2.4-3.8) mas Test Species: NA3/hu/R MAHA Test Date: Aff& ~8-8Y ur. vi RIZZO Analyst(s) r= 2 - suste oA HR Exposure Type: (Exposure/Test Conditions) Static Recirculatory_ Static Renewal Plow-Through_ Diluent: Aerated Well Water (Initial 0.0.8.7na/1s PHA,2): Temp. ZX CRE) Water Hardness:__2/4) ppm Hardness Classification: AKED _ Dissolved Oxygen % Saturation: . Control(s) Lowest Experimental Dose 1.0wall Highest Experimental Dose L3wall PH Levels: 48-hr 100 Joo. 100 96-hr -- _ EExxptoesnudreed _ --- Control (s) Lovest Experimental Dose |.Owgll Highest Experimental Dose 3ngd Test Temperature C (F): 48-hr 83 8a a4 96-hr _ [---- EExxptoesnudreed -- ---- Bhotoperiod: 4s-hr 96-hr EExxptoensduerde zen) tcl) _el) gog073 Sixteen hours light with a 15-30 minute transition period. , - BESTCOPY AVAILABLE ~~,ZEiNvVzIzROoNnMeEwNmTAsLs,LAwBaOsRoAmTaOzoRrYy DEFINITIVE (FULL-SCALE TEST) wee restfBu AuneSame MATERIAL LUDKr Ds ffr3400:5) bnog sos TEST otap RoW REPLICATE COMBINED Leo's J Rew REPLICATE 50's COMBINED . LcOICssooo''ssn(9(95r5%% rCONdFIDmENCnE LiicrmmIrrsss)) * PROBIT ANALYSIS Spel (2.8 4,0) FB. Zmy (2.6-3.8) 3,2 mg (2.-9 3.0), *MOVING AVERAGE ANGLE METHOD 3g" (z.8-5.8) 3.1- m &I--3.4) 3Z5ma. ll -- 2.9-3.4) ~ comming ; 006074 __ * ian BEST COPY AVAILABLE AQUEANTVIICROTMOEXNICTIATLYLDAABTOARTSOHREYET WwWBLIO Tyre msl rh SiggrvaterianTe YR Si Shon, as Tanlk Toest Oxs[antenag=hus ] Total eli.0c[itTotsar 6s [pao ve, ota Fors Vous Fou Ho Age: First-Instar young (0.5-1.5vm) No. 4ontoms ber treatment LO. Test Chamber: 250ml glass beaker Test Solution Vol.: 200ml Test Solution Depth: Gem. Time Started,`Z.. Date DilutionWeter, nm Started ter i/ 54 (Tews. 237 Date C, Completed 0.0, \ PHB.ZY Investigat (Full Signatirp) 2 Z "oo (95% Confidence Limits) TXCAT ANASSS BEAR: -5.8) wolf Mvihe HAGE ARGC (2.75 Sue v0 tour p.o, [ror Tpr. No. Added No. Dead| p ppm No.Dead Pp! No,Dead b ppm |INo, Dead bH fpp E I y A lwo zo 0 pea TH iAAN 2, OSE | 2 Aleaf ze 70 TT 2 a, 37a 70 Tedd | TTT L I T r TT TT mewn . wee||[ 75E 7 / 2 5 17 T fi I comn7Zt eres t :ff stot 5 Sd. Lrg, verfe KB Lutil =n Llriatsd sitdot, 3.2. 8 aud Lolo Effects other than Mortality ocr --" I= | Total No, Affected No. Symptoms or Reactions i 24-Hr, I 48 Hr, 72-Hr, 96-Hr, L r J r [1] Lr rr [1 C--O TTT BESTOOPY AVAILABLE rn coms WBZ) EOLEE AQUATIC TOXICITY DATA SYEET Tyre Tes Tost. Orsantsi fg SR MaterialTe sors EY foo Cron SR G3 D rate ve, Ase: Test CFhiarmsbte-rI:nst2a5r0a1yougnlagss(0b.e5n-k1.e5rom)TestNo?SolutCieognsnViodlf.i:odrs2tOrnelatTensetnt__j) Time Started (BIAyyS Date Started Dilution Weter_Carbon:filteredwal) water Ls[Bfbare (Tom..25 Solution Completed Depth: 6gm. 4 Investigaty C, 0.0. , MEzY Z Lz LC, (95% Confidence limits) errs 50, Tank EE 5 Tota1 Rows0.0] Totus Wows 0.0 Total 1%" p.0, [h5o%rtFou p. r em [ET 2 Se ff cM Z aa I 27 E2180 A 00270 77 res soo Fla eo m7 ee --I i ma rs Se ------H -- e 7 |OZ = H mt tr || 2m |or : wa 12 75 KT HH TS feTe | Comments*:/7, 4 Wd Lessard attod 2, = sil 2 Mag 30 To 8, and [Z.bo wl Effects other than Mortality * Quay I= [ No, 1 ymptoms or Reactions f 24-Hr, Total No, Affected 48 Hr, J2-Hr, 96-Hr, 1 Lr r f [nr [1 1 Comments: . _-- mm eteeseseres