Document NYMmR84zeQXJn60ZRqELpQkp
Attachments to Letter to C. Auer dated May 18, 2000AR) Je Studies and Other Information on Certain
7
Perfluorooctane Sulfonate-Related Compounds
12. N-MeFOSEA | N-methylperfluorooctane sulfonamidoethyl acrylate
Genotoxicity
1) TEevsatlu(awtiitohnoinfdethpeenMduetnatgerenpiecatA)c,tNivOiTtyOoXf,T-P5ro8j6e9ctiNnot.he1A1m59e6s5,Sa3lMmoRneeflelrae/nMciecNroo.soTm-e 5869.1 (FMZ-3559, Lot 2408, wide-range), April 20, 1994
2) HEvuamlauantiLoynmofpthohceyAtbeisli(twyitohfTi-n5de8p6e9ndteonItnrdeupceeatC),hrNoOmToOsXo,mePrAobjeercrtatNio.ons11i5n9C4u3l,tu3rMed Reference No. T-5869.2 (FMZ-3559, Lot 2408, wide-range), June 5, 1994
3) MEvuatlautaitoinonToesfttwhietMhuLt5a1ge7n8iYcMAoctuisveitLyyomfTp-h5o8m6a9Cienlalns (IwnitVhitriondMeapmenmdaelnitarnepCeeatl)l,Gene
NOTOX, Project No. 115954, wide-range), April 20, 1994
3M
Reference
No.
T-5869.3
(FMZ-3559,
Lot
2408,
a
.
005581
REPORT
EVALUATION OF THE MUTAGENIC ACTIVITY OF T5869
IH THE AMES SALMONELLA/WICROSONE TEST (WITH INDEPENDENT REPEAT)
NNOOTTOOKX SPurbosjteacntce11356516956 ~ page 1 of 23 -
RECEIVED
NOV -n9h1494
ToACULOGY
605582
T-5869
-
NOTOX Project 115965
-
sTaTevenT oF ue coveLIRICE - nnn
VOTO 5.4. *s-Hertogenbosch, TheHetherands
TThoetesntudeybdeesncriobf"ed fn this report vas conducted 1compl ance with the most The OECD Principles of Goad Laboratory Practice rien are essentially in conforatty with: TRhoe aUntitaednSbtaaterseF?ood and Orug Adwinistration. Tite 21 Code of Federal TFheederUenritendepuSttaatteisonsenypiorroenm6e0n:tal Protection Agency (FIFRA). Title 40 Code of TFehceerUantiteRdeSttaatiens sEnvpiarrsonmoeonp:tal Protection Agency (TSCA). Title 40 Code of riothrt:he exception that the stability of the test substance fn the vehicle fs
stu otrector
Tog. .3. van de vaart
em
Date: Yr
pez
005583
T5869
-
NOTOX Project 115965
QUALITY ASSURANCE STATEMENT _------
NOTOX B.V., 's-Hertogenbosch, The Netherlands.
sSpteucdiyfipcropcreodcueressseswewreeresubaljseoctintsopepcetreioddiact pienrsipoedcitcionisntearnvdalgse.neral non study Tanhdis rreespuolrtts awacscuraautdeiltyedrbeyfletchte NtOhTeOXrawQudaaltiat.y Assurance Unit and the methods
DATES OFAUQDAIUT_SINSPECTIONS/
REPORTING DATES.
24-01-1994 26-01-1994 15-03-1994
24-01-1994 26-01-1994 15-03-1994
Quality Assurance Manager
C.J. Mitchell B.Sc.
Date: 20 ~L- At
- page 3 -
0605584
T-s86 _REPO--RT A-- PPROVm AL
STUDY DIRECTOR:
NOTOX Project 1196s --
Ing. E.3. van de aart Y=
vate: sp/ou 9
MANAGEMENT:
TOer.chnIiCc.al EDninriengastor
vate: 20] ou liggu
--
605585
T-5869
NOTOX Project 115965 --
PREFACE _-- Sponsor
Study Monitor Testing Facility
Study Director Technical Coordinator Study Plan
3CManaBdealsgtiruanat- 1Ch1emical EBC 8Be-l2g0i7u0nZWIINDRECHT Mr. R.H. Cox NHOaTnObXakBe.nVw.etering 3 5Th2e31NDeDthe'rsl-aHnedrstogenbosch Ing. E.3. van de Waart G. van Cort. CSotmpalertetd:: JFaenburauraryy250,4, 11999494
TEST SUBSTANCE _-- Identification Description Batch Purity Specific Gravity Itenssttrsuucbtsiotnasnceforstorage coSntdaibtiilointsy,under storage Expiry date S4tahbolures foirn vaethilcelaest
T-5869 Crean solid 2408 95% 15
At room temperature fn the dark
Stable January 01, 1996
Oimethylsulphoxide: not indicated
VEHICLE -_-- Tqprhuieaolritteystto (usMseuerb.cskt)a.nceTeswtassusbusstpaenncdeedcoinncednitmreatthiyolnssulpwheorexipdreepoafresdpedcitrreocstcloypic
- page 5
005586
T5869
NOTOX Project 115365
e cutoELINESmt----m-- m -- e -- e TShueidesttuidnygs.procedures described in this report were based on the folloring - OGTorurgiiadcneoilTsioangteyis:onfoSrafolrsToensEetcliolnnagosotfyfcphCCihome-muoirpcieaurlnast,iRoevnGeuriasdneedlMiDnueetvaetlnioo.opnme4A7n1ts:s(a-OyEG.CeDn)s(,taidsoOpEtCeOd - EJ EEuCloDpieraenctEicvoer-- neos6t7c/S4C8o/mEmEuCn,ityPar(tEEC6)1,HoDtihroedcstivTeor92t/h6e9/DEeEtCe.raAtmmaemxionV oobf the 1RT5eo5vx2ie)cr:istey:utBa.t1i4o:n"AOstshaeyr".EffEEeCctPs-uHbultiacgaetniiocnitnyo:. LSe3l0a5onTeeddivas.EteysahDirsmuesrbeerm, -
A-- ReCHrIVING --e-- rr ---- NPrOoTtOoXca8i.,1. rweip1o1rt,arctehsitv.eartthieclfeollroewfienrgencdeatasafmorpastndTearsatn 1u0thy.ears:
oaaerIve
Purpose of the stusy
Tt0hyeshiionndbuuerccietuinrveebvaecrotsfeertimhauilstatssittrouandisynsyianstoatopgreoendvsuacloeufathhieisstttihidediinnteee.s-trienqsduueibprseintdnaegnnctesSafErororansTiintisssoafbility
preRsSeenRcTeTSo-foragRaentyaobnost.icTsneysatsesaay.(Sb9e-sm.1xb)o.ndurtes nthe shoo: amy Sone
Justification for selection of the test systen
aTThuheteagAbeanncetsiecrtiaeasclttivshtiartsayibnoesfeneamsphlioodwenyedrtoawnegbreeeoacfarpcaahpbeilmdiecaaonlfd
CIonmepxopuenndssi:ve indicator for Getbcting both frameshift
the and
Base-pair substitution autagens.
page 6 -
005587
T-5869
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MATERIALS AND METHODS
NOTOX Project 115965 _
TEST SYSTEM _--_-- Test System Rationale
Source
Salmonella typhinuriua bacteria
Rreeccoogmnniezneddedbytestthesyisntteemrna(te.igo.nalEPAg,uiOdEeClDi,nesEECa)s. the
OBre.rkeBlreuyc,e and TAL00;
NU..S.AAn.es,(TUALn5i3v5e,rsiTtAyI53o7f 1993)
aCnadliTfAoSr8n;ia1a5t87
The characteristics of the individual strains were as follows:
strain
Histidine mutation
Mutation type
TTAALS5B37
hRiiss0c33005726/R-factor*
FFrraanneeshsihffitt
TATAIL0S035
hRIiSsGG4i66/R-factor
BBaassee--ppaaiirr ssuubbssttiittuuttiioonnss
*: R-factor = plasaid pKMIOL (increases error-prone ONA repair)
Each tester strain contained the following additional mutations:
gEIaflaT: dmRuietkeaapttiirooonnughiinn(ntdihetefreagctateliavcerteo1dsiaeptoempseostlaybsaoclcihsamride cellcoat)
BVlros:: dJoesfsecotfivethebioetxicnisisoynnthreespiasir syste (deletion of the ultraviolet. repair 8 gene)
S(-t1o9c6kCc)u.ltSutrreasinosf wtehreeforuergulsatrrlayinschewcekreedsttoorecdonfiinralitqhueiidrnihtirsotgideinneTrAeLq0u0i)r,eaeUnVt-,sencsriysttiavlityvioalnedtthseensniutaibveirtyo,f aspmopnitcainleloiunsrreesviesrttaannctes.(TAS and
CELL CULTURE
----------------------------------------------------
Pbarcetpearriaatlionculotfures
Samples of frozen stock cultures of bacteria
-
w(e0rxe01dtrnaon.sf2e)rreadndiintnocubeantreidcheidn anutsrhiaeknitngbbraotthh
0(.307.C,Of105.04spaat),700untniml (t1h0e cceullltsu/rmels).reFarcehsehdlyan
gtersotw.n cultures of each strain ere used for a
Tpage 7 -
005588
T-5869
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NOTOX Project 115965
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Agar plates
Top agar Environmental conditions
AaTiggtaaerrrspRelda"1it8uensg.p(uGsrliu9fciocesa)de caaggoaanrrtaim(ne0eaxdoi1uan2,5cocao1ndteag.ilnu1ec30od)sepeirn V5(go%)gebViooBgtoeinlnn,era0n.d0M.0e.d6ainuamggEhai(ms1et)r,i,di10noeH. .lo1c95o6s,. 005 pAucreitfyilcoartni]otnhinaandsesoomfepEsrchoerpischriaiec3o.st.:Hopra.rtial
Chen. 218, 97-106.
VpSoaugmrepi?lfeiBseodnonfaegra3raNveadsttuospheaatgaecrdonwtteaoriedniintsgrsaon0ts.vte6eXrtrh(eewd/va)gnaero. 1t0ub8e1s VGelraessauttuobcestavweidthfnoreta2)0 Bcaipns.atTo13p00aLg,ar aAt1ht1e3e7ixnCpc.eurbTanhteeinotnt.semwpeerreatucraerriweads soounti tinoretdhecdutarnkg
- REFERENCE SuasTAICES VNTRegeathiveTceonatTfroelt:he test article.
Positive controls:
VS TitAhoHuStran int metae bsooldiicut naazcitdietve ato(iswo)nr C(F(-lsuE9k-t a1x))L: opiTaotg e Ssoatvnenet
TASS THs
SG{aaaumanniosnnsoyeaencirnnieidim(nOieW))(9(4S6t)gsa)
4g sane fou sane
TAI methyTasthanssuironate. 55) 0 ug ow
(Merck)
t TiAtDhSmSr,ametaboleitc eacrtI ivation (9-10): attopiate solvent
weTA,ISH7 2-aninoanthracene (24%)
5 wg ouo
THi0O (2s-iagsst)noanthracene (284)
osu owo
SpDoMlSrvOeSarliynie t+=ssGfioTzretorheyfTesruoelnpcgheosxtsaiuTdbiaesnteaao.nfc(lesWsepaetcttarlosPchoaprieca quNaeldi.ty"8.1(.H)are).
"pages -
005589
T5869
NOTOX Project 115965 -
mMETr--ABOrL--IC--ACT--IVA--TIO--N S--YST--EM ------------------------------
Preparation of 59-homogenate:
RoratSpMrvaegruemDiacrwolseoymarlatse,nzywmhiecshwweerreeroobuttaiinneeldy pFrroempaBrReLd, fSrovmitasdeurlltndm.ale Wistar
TTihanebtoraranaptteearartlyosncewoaenlrdlieytihowoniusts,hedaasatsdoelNsuOctTriOioXnbeidn(20ia%nswtp/hevec)taSlOOfPrsAo.roomcTihuaenrderr1at3s5s4twaen(5rd0ea0r.dimnajjeicgtedbody
Ws(aEtcIhrGeiNyfti)wceeri)en. CdToehrnenedo11i1av.ecrcseFsisvoeftodthaeyfsorodaTtastfeorwr,eraettheTryeeamsowtveerde12kahiso1eu1pretsdicbpryrieycd.eedcaianpnidgtewbaisohnesd in
cNi0na1'-d3EDv(To0Al.Cu)meSsusbtseoefrqtupieehnots0ip.yh1atHtehesboudlffifuvenerrspihowteshrpehaamtiePnoctbetudefrfeirhnoma(opgbhelne7i.nz4de)arr.eoaTnnhdteaihonhmioonsggaenmoir1seesdmt
:
vianstocesntterriilfeugeamdpufloers,15whAiinchatver5e00s0tog.redTheinsuTp1earuniadtannitt.ro(g65e)n w(a2s.96teracnyeiornce
Preparation of s9-ntx:
S$99--aniixx 5.5 ul
awcqaousnatapbirinedepedasrtpe;edr2i1m0umlemd10i:.a5te3Hl0ynsgobdeiNfuAomDrPephuaonsdsep1ha5an.dt2ekbeHup)ftfgelornocpoiHscee7-.6d4-u;prhion3gcpohhtahc0ee.0t8eisnt.
HfsEroCa1,cutfiioosnno,slutwwiheoirnec;hni1axsem1d a0adnd.ed3d3Fa1f1ttKeCeIrr fs(io0il.tu2et2rioysnat,je-rsaintldairz0ia.it5jizoaen1d
5(9a.parTth.efaoboovethe of the S9-nix
59-
components).
-- EXPeER-- IeMENT-- AL PrR-- OCEr DU-- RE -------------------- Selection of dose levels SwtieetslhteecdsttiroiannintorfiTApaLln0iDca,adteebqouta(httehiwsirtahpnrgeeanldionfiwnidatorhsyeosuttweSas5st-mwbiaaxss.edrEeipognohrttaedpcoranescleinamtiprnaaatrrityonotsfesttwneere tFeisrtstaretxipcelrefaeunstedofintthheeasuutbasgeeqnueesnits masustaayg)e.nesTihse ahsisgahyestwasco5ncemng/tpriaattieo.n of
"pages
005590
T5869
NOTOX Project 115965
Direct plate incorporation assay *:
Fstieuvsbetsetdadnicbfeoftehrweenrwteithdteossatenesddw(iiwtnihtohturtiapp$lp9ir-coaaxitixematiiennlyceaacchhhalsfst-trlraaoiignn,.steTiphnse)ttwoeofstitnshdeuebpstetensadtnecnet was effxropeleslrhoiwmibenangcttses.roilaTulotpiocnuaslgtaurwrereien(1st0uo9gpcceagesalsrlisv/etmlulyb)esadodwfeadsonemtoeloft3edmt1heantdotpeshteaegaratre:dstr0ta.oi1n4s5m,lC.0o.f1Them1 ocnfaosnea-acdoftiilvauactttiiioovnnatoiafossntahyaess)st.aeysstT)hesourbisnt0ga.r5necdemi1enft0ns.1ONwSeHDr,ephomasinpxdehdaetieotnhebrauf0fV.eo5rrtem(1xinaSnc9da-smeitxheof(in dscaoornlktiedniattfico3fa7tCitohneforotfop48tahehg.artoApfttuebaregarwt,ahsisthpepoeurprileoaddteosnrteovweerrateasntetulrenc(ethdiivsetainddaignaierncpuilbnaadtteeep.dendAiefnnttet)rhe colonies were counted.
*)
cAnaersc,inoBg.eN.n,s WacnCdannnu,tagJ.ensanwdiYtahmatshaekiS,alEs.o,ne1l9l7a5/,saMneatlhtoadns mutagenicity test, Mutation Res., 31, 347-364.
mfoircrodseotneecting
*) Mnaurtoang,niDc.Hi.tyantdestA,nesM,utaB.tNi.o,n R1e9s8.3., 1R1e3v,is1e7d3-m2e1t5h.ods for tne Salmonella
Erratus, 1963, Mutation Res., 113, 533.
Colony counting
WTihtehraenveArrttaenkt mcoodleolnfe8s80 (choilsotniydicnoeunitnedrepeonrdemnatn)ualwleyr,e ciofunlteesds atuhtaonma4t0ically
pcroelcoinpiietsatpeer toplaitneterwfeerreeprweistehnta.utPolraatteesd manually.
wciotlhonysucfofuinctiienngt
wteesrte
acrotuinctleed
ACCEPTABILITY OF ASSAY
aA)n ATnheesnetgeasttivies ccoonntsriodleredadtaacc(enpustbaebrleofifspiotntmaeneetosustherevfeorltlaonwtisngpecrritpelraitae:)
sfhorouledachretaessotnearblystrbaeinw.ithin the laboratory backgroundhistor ical range
b) Tshteraipnossiwtihviechcaolntsroolrecahseoaniabclaylsarsehouwiltdhipnrodtuhecelarbeosrpaotnosreys hiinstalolrictaelster
Fruarntgheerdnoocruem,enttehde mfeoarnepalchateposciotuinvteschoonutlrdolbesuabtstlaenacset. two times the
)
Tcohnecusrerleenctt.edvedhoiscelercaonngetroslhogulrdoupincmleaund.e a clearly demonstrated by the preliminary test with strain
TtAoLx0iOc
coronscheonutlrdatieoxntenads
to 5 ng/plate.
page 10 -
005591
T-5869
NOTOX Project 115965 ~~
DATA EVALUATION AND STATISTICAL PROCEDURES -_--
ANotefsotrmaslubhsytpaontcheesiisscotnessitdienrgedwasnegdoantei.ve ir:
(not
mutagenic)
in
the
Ames
test
a)
Tishenottotaglreantuemrberthaonf trweovetritamnetssthien ithout metabolic activation.
asnoylvetnetstecrontsrtorlainvalaute,anywicthoncorentration
b) Tihnedenpeegnadetnitvleyrreesppeoantseedsheoxupelrdimbeentr.eproducible in at least one
aA)tIertsetveisrnutdaubncstetssanwcaietthilsearscetosnpseaic2dt-efrtoeolddt,pheosdnioutsmiebveerrel(aiutntedadugceeindniccbr)yeiantsheethfsneolAtvmheeesntnutmceobsnettrriofol:f in
aHnoywevoefr,theanytemsetaenr sptlraatiensc,ounetitohefrlweistshthoranw2i0thoiustcomnestiadbeorleidc significant. If the test substance showed in the first test
tacotibveatniootn. only a
pthioetshitfiidvrosestesrteejssuptso.tnsebelaotwoanendorexctweoedicnogncetnhtorsaetisohnosw,ingtheposaistsiavyewaesffercetpseatiend
b) Tihnedeppeonsdietnitvleyrreesppeoantseedshexopuelrdimbeentr.eproducible in at Teast one
eTnhteerpreicnetodintghecrFiintaelriaevwaelrueatinoont adebcsiosliuotne. and other modifying factors might
- page 11 -
005592
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NOTOK Project 115965 ~
ResuLTS
e-- TOKe ICI-- TY OF--THE -- TEST S-- UBSTA-- NCE ---------------- TSofohlevceosnlutorvniicveoasnltornoofthptelhaecpelTsaA:tLeOsD ccounlttauirneinwgastdheetetrensitnesdubsbtyanccoempvairtihngthtohsee.nounsbetrne vBuagos/tphlnoaIttn.e rthe(edTuacbaelbdesenU1)cp.etBoaansdaenddproeInsnecnlthcueedsienogfcatSte3as-,taixstuhbetshtetaenssctu.er.vsicuvobanslttaennoctferaswttairsaa.nisnecoTtfAeId0SO00up0 p5res2enndceincolfud$i3-n3g1xa. concentration of 5003 u/plate Ih the sssemce and
es TmHE A-- MES S-- ALMON-- ELLA/-- MICRO-- SONE -- PLATE--TEST-------------- weTinattbhilrees7.3d18o6sa5en.drAa2nlgsehbaawocfttehtrehiearlteesssuttltrssauibnossftastnhhcoeew,eSdatisncoe.ngeanltoilvaed/oasirece.drrpoeesnroaanstese.dp.olvaettrewo-tfneeosltd, eSixnctprheeiranismeea.nutrisn.latbhTehoernautnmoebrgeyartibvioecfkgarrneodvuensrdttarhnaitissnt-osinrp'iecctauiol?.icirannpdgoeespsi.etnidIvennedtisccyaanttireneegpnattvhaaagjtueisnevere fFeasntctCioonnedditipornosperwleyr:e optinal and that the metabolic activation systen
- concLuston
nnn
B1a5se1d0tonmuttahgeenriec.sulitnstohef Atnheiss sStauTdayuneiltlaf/saiccornocsloundeedastshaayt. the test substance
page 12 -
605593
T-5869
NOTOX Project 115965 --
TABLE 1 MTUESTTA!GENIC RESPONSE OF T-5869 IN THE AMES SALMONELLA/MICROSOME PLATE
Experinen1t
----e--e --------------------------
D(ousge/prate)
M3SetarrnaeipnlnsiucaabofteerSop.lftaytprehesivseur(rt+ia5un.nt0.()Hiwsit)thcdoilfofnfeerse/nt
eeet--------T--AS-- S ---- TAS--S ----Tas-- s ----A1--00 ------
Without 9-mix
103 10303 1030303 5300000 SPooslivteinvte ccoonnttrrooll!
67::43 58:43 5:1 26751:122
55:i22 86::35 7:4 3569:2239
1163.i31 119i:36 18:4 6w21.3e320
11306 :+ 214 120::52 1d2i:a6 113m:1:0 113732:3219
103 10303 1030303 53030000 SPooslivteinvteccoonnttrroo1lt
ith 59-ntx
58::11 589:::122 2480::38
56.x24 67::32 541 50774.118
211.:52 1166::55 16:2 414161.328
1133s+ 129 11886s352 1158::85 11530:51108 61001s.n31
*10T.e1st,msubosutsaonce precipitated slightly on the plates
- page 13 -
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NOTOX Project 115965 Z
TABLE 2 MTEUSTTA.GENIC RESPONSE OF T-5869 IN THE AMES SALMONELLA/MICROSOME PLATE Experinent 2
(Duogs/eplate)
3Mearneplnuincbaetre polfatreesver(txan5t.0.()Hisw*i)thcodliofnfieerse'nt Strains of S.typhisuriua
TALSIS TAIS37 TASS
TAL00
313030 313030000 5000 sPooslivteinvteccoonnttrrooll:
5Bs:14 1100:% 44
8:2 2746:+228
193s:51 180::32 10:3 5777++839
1189:443 2292502s7 29:3 62431+:528
15191::204 1165834s1174 129% 9 111640344388
313030 130303000 50002 sPooslivteinvteccoonnttrrooll:
1724:15 98::43 751 217844324
With $9-mix 59::24 225::43 57::32 2266::25 7:3 Bel 6685:.48 22535+.37
11326841i3 113346:1147 17:5 14909:$1080
0T.e1stmsub0MsStOance precipitated slightly on the plates
page 14 -
005595
T-5869
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=
APPENDIX 1
tEhveidefnocleloowfingtesptrecairptiitcalteiopnreccoidpei.tate on the plates 1s recorded by addition of
r PeREeCIP-- ITATI-- ON ceove-- e --------------------
oereefin--itio--n ------c--hara--cter--isti--cs ------------------
Slight precipitate DHcoiowusentvtieinrng,guistohfheedthpebryepcinapotiteti.acteeabldeocsprneocti.piItnaftleuenocnetshuetoprlaattee.d
Moderate precipitate Heavy Precipitate
pDDliiassttteii,nngguruieisqahuneeiddrinbbgyy taahemlaarprlkgaeetdesaaomtuoonutnbteofohfanpdrpercceiocpuintpttieetsta:steaonnthtehe
ee e er-- Plate-- , makin-- g the -- required-- hand c-- ount g1-- PFIeerTee-- .--
- page 15 -
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NOTOX Project 115965
-
IAndPiPviEduNaDl IpXlate2counts; (following pages) sEtxrpaeirniaent Tass1
vITHOUT 59-400
pate 12 3 wm wm
ooseug/prate)
15103000
n0a 0asi s33 6I51sE3
S5a35o0:
LPE 72 HsHii
SPooslivteinvtecosnotnrtorlo1 7826sgmae7.i2i
VITH 9m
Mate 12 3 mew ow
beseGuarpiate)
11050303
F 5ios0I s 67 5sHiE1
33000000
sSoua sog ssiit
sPoolsvietnitvecconotnrtartol 3s1o2uuss a05}. 8
Test substance precipitated stiantly on the ates
RE
005597
71-5869 sExtpreariinnent TA15371
NOTOX Project 115965
WITHOUT $9-MIX
plate 1 2 3
Dose(ug/plate)
313030
65 5473
13030300:
27 53 13
5000+
ous
SPoolsvietnitvecconotnrtorlol
3264 3706 3809
MEAN SD
55a:22 86::53 7%
3596 +3293
WITH $9-MIX
plate 12 3
Dose(ug/plate)
130303
59 24 67
133030004
37075 98
50004
565
SPooslivteinvtecocnotnrtorlol
4868 5136 5216
* Test substance precipitated slightly on
MEAN SD
56.x24 576::5123 5077s+181 the pates
- page 17-
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T-5869
sExtpreariinment
To81
NOTOX Project 115965 ~~
WITHOUT $9-MIX
plate 1 2 3
Dose(ug/plate)
130303
7 B1B2 1132
130303004
2u6 u16 o15n
5000+
5 16 2
SPooslivteinvtecconotnrtorlol
63178 58169 63195
MEAN SD
6B:ol3 4vsie3 Bs 4 6o1 e+ 302
WITH $9-MIX
plate 1.2 3
MEAN SD
Dose(ug/plate)
130303
2u0 2o62 210
21a8:. 52
10330300:
222 117 11
66:: 535
50008
8 16 15
6: 2
SPooslivteinvtecconotnrtorlol
4875 41193 4210 a116:: 328
+ Test substance precipitated slightly on the plates
- page 18 -
005599
T-5869
NOTOX Project 115965 -
sExtpreariinnent
A1001
WITHOUT $9-MIX
plate 1 2 3
Dose(ug/plate)
130
11203 113388 115012
10303
221112B7L w16r7
333 1000
11662 11252 113326
353030004+
113338 115333 11248
SPoolsvietnitvecocnotnrtorlol
1318530 1136389 1139750
MEAN SD
1u3o6 +142 2102:254 1239:s6a #833:+150 1317623 21 1219
WITH $9-MIX
plate 1 2 3
MEAN SD
Dose(ug/plate)
103
1020 112873 112345 13330 +2129
13030
11337 113282 u1s3 3u6s ++ 25
1030303
114328 115401 112561 u1s0:i8s
53030300:0
113259 116282 115835 11530 111140
SPooslivteinvtecconotnrtorlol
613672 518862 156861 7001 +11311
+ Test substance precipitated slightly on the plates
page 19 -
005800
7-586
sExtpreariinnent
TA15352
NOTOX Project 115965
WITHOUT $9-MIX
plate 1 2 3
Dose(ug/plate)
313030
B6 s6 46
103303004
57 1135 18
50008
6 8 10
PSooslivteinvteccoontnrtorlol
29%8 2834 227
MEAN SD
85+41a 00::44 8:2 2746 ++282
WITH $9-MIX
plate 1 2. 3
MEAN SD
Dose (ug/plate)
130303
89 108 1&8
27:451
130303004
0 E5 TT1
98:i3a
5000:
857
751
SPoolsvietnitveccoonnttrrooll
1859 2175 2538 21874+324
* Test substance precipitated slightly on the plates
- page 20 -
005601
T-5869
Exspterariinment Th15372
NOTOX Project 115965
-
WITHOUT $9-MIX
pate 1 2 3
Dose(ug/plate)
1300 103303004 5000 SPooslivteinvtecocnotnrtorlol
B8 U8 1o0s ssz2 791 8 13 8 6795s 51u6 s53
ean so
1ER 3s 08::23 0:3 5777.Le3s
WITH $9-MIX
pate 1 2. 3 ean so
Dose(ug/plate)
130303
0us2 37
59:.42
130330004 50000
83 903 38 78s
57::32 7:3
PSooslivteinvteccoonnttrrooll
53 76u0n 55110 6658s= 864
+ Test substance precipitated Tightly on the plates
- page 21 -
005602
T-5869
sEtxrpaeirninent
Tse2
NOTOX Project 115965
-
WITHOUT 9-1 pate 1 2 3 wean so
Dose(ug/plate)
1301500300
81 18
222B21
0F9E:.23
3S30500::
33%anon 3Bi:3
;
Psooslivteinvteccoonnttrrooll
62 so1b9 eaat en2s .is2i
wT 9.x
pate 1 2 3
)
Dose(ug/plate)
1303100300
2mvon3 oo2ma 2=% i:442
E33s%:
2 2aoa 2FE% is
SPootsvietnitvecconotnrtorlol
0nw 226 42 2239 173
Test substance precipitated sTightly an the prates
page 22 -
605603
T-5869
NOTOX Project 115965 -
sEtxpreariinment
TAL002
WITHOUT $9-MIX
plate 1 2 3 Dose(ug/plate)
MEAN SD
313030
116605 115954 116535 5m9os+ 24
1000 33300
116502 115678 114808 6583:+ 117
50004
139 121 127 29: 9
Psooslivteinvteccoonnttrrooll
1105254 1111586 1218760 1114630 3+ 388
WITH $9-MIX
plate 1 2 3
MEAN SD
Dose(ug/plate)
313030
11215 113229 115114 113268:+ 1143
130303004
u11e 113s8 114238 B13s6 L+ 147
50000
1 1:2 137 Bs
PSooslivteinvtecocnotnrtoroll
319328 51981 143828 419309 ++ 1008
+ Test substance precipitated slightly on the plates
- page 23 -
005604