Document NR9ojgDVd1By8J76JZo1ME9Q

HALOGENATED SOLVENTS INDUSTRY ALLIANCE 1225 19th Street. N.W., Suite 300, Washington, D.C 20036-2411 |202) 223-5890 May 23, 1991 j!t\ JIW"6I99I WuL...____ TSCA Document Processing Center (TS-790) Room 201 Office of Pesticides and Toxic Substances U. S. Environmental Protection Agency 401 M Street, S.W. Washington, D. C. 20460 '" Re: Final Report of the Rat Neurotoxicological study under the consent order for 1,1,1-Trichloroethane, 54 Federal Register 34991 (August 23, 1989); Docket No OPTS - 42059C In accordance with the terms of the above-referenced consent order, HSIA submits six copies of the enclosed final report of the rat neurotoxicological study on 1,1,l-trichloroethane. Sincerely, Enclosures cc: F. Benenati (OTS) c. Roman (OTS) Thomas A. Cortina Director of Administration SL 030708 Printed on Recycled Pace' Volume 1: Main Report, 129 pages Volume 2: In-life Data, 209 pages Volume 3: Pathology Data, 83 pages STUDY TITLE Neurotoxicologic Examination of Rats Exposed to 1,1,1-Trichloroethane Vapor for 13 Weeks AUTHORS J. L. Mattsson, R. R. Albee, L. G. Lomax, M. J. Beekman and P. J. Spencer FINAL REPORT May 21, 1991 SPONSORED BY Halogenated Solvents Industry Alliance PERFORMING LABORATORY The Toxicology Research Laboratory Health and Environmental Sciences The Dow Chemical Company Midland, Michigan 48674 LABORATORY PROTECT IDENTIFICATION K-001716-091, K-001716-091A, K-001716-091A2 K-001716-091B, K-001716-091B2 SL 030709 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 1 of 3, Page: 2 COMPLIANCE WITH GOOD LABORATORY PRACTICE STANDARDS To the best of my knowledge and belief the study described in this report was conducted in compliance with the following Good Laboratory Practice Standards United States Environmental Protection Agency, Title 40 Code of Federal Regulations Part 792, 1 July 1989 Edition Japan Ministry of Agriculture, Forestry and Fisheries, 59 NohSan, Notification No. 3850, Agricultural Production Bureau, 10 August 1984 Organization for Economic Co-Operation and Development ISBN 92-64-12367-9, Paris 1982 Study Director Sponsored and Submitted By: Director The Toxicology Research Laboratory T. A. Cortina, Director of Administration Halogenated Solvents Industry Alliance SL 0307X0 1,1,1-T subchronic neurotoxicity The Dow Chemical Company ProjectID: K-001716-091 Volume 1 of 3, Page: 3 QUALITY ASSURANCE STATEMENT STUDY TITLE: Neurotoxicoiogic Examination of Rats Exposed to 1,1,1-Trichloroethane Vapor for 13 Weeks. This study was examined for conformance with Good Laboratory Practices as published by the U.S. Environmental Protection Agency, and the Food and Drug Administration. The final report was determined to be an accurate reflection of the data obtained. The dates of Quality Assurance activities on this study are listed below. Study Initiation Date: 9-15-89 Study Completion Date: 5-21-91 TYPE OF AUDIT: Preliminary protocol Final protocol Facility Inspection Study Conduct Addendum 1 Protocol, data Draft report DATE OF AUDIT: 9-15-89 9-21-89 5-21-90 6-12-90 10-23-90 DATE FINDINGS REPORTED TO STUDY DIRECTOR/MANAGEMENT: 9-15-89 9-21-89 5-25-90 6-13-90 10-24-90 5-13-91 5-16-91 ARCHIVING: Raw data and a copy of the final report are filed in the testing facility archives. At T. S. Gushow, B.S. Quality Assurance Health and Environmental Sciences The Dow Chemical Company 1803 Building Midland, Michigan 48674 030711 SL 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 1 of 3, Page: 4 SIGNATURES m /yiA y 9/ 7j. L. Mattsson, D.V.M., Ph.D. /Date y Study Director L^=z-U L. G. Lomax, DfV.M., Ph.D. /Date "RD. DRyvAAlIbUeaea, M\A.SC. ~yy?4 M. J/Beekman, B.S. `S/n/'ii /Date 0-fUltU ^TrjdujS/ encer, B.S. /Date Reviewed by: J. P. Maurissen, Ph.D. /Date SL 030712 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 1 of 3, Page: 5 TABLE OF CONTENTS VOLUME 1: Main Report PAGE SUMMARY.................................................................................................... 7 CONSENT AGREEMENT.......................................................................... 8 INTRODUCTION........................................................................................ 9 TEST PROCEDURES.................................................................................... Test Species and Husbandry............................................................... Dose Levels and Dose Selection........................................................... Test Material......................................................................................... Exposure Chambers and Atmosphere Generation.......................... Design Summary.................................................................................. Body Weights......................................................................................... Observations......................................................................................... Surgery................................................................................................... Postexposure Tests................................................................................ Waveform Analyses............................................................................ Neuropathology ................................................................................... 10 10 11 12 12 13 13 14 16 17 19 23 RESULTS....................................................................................................... Test Atmosphere.................................................................................... Overall Perspective................................................................................ Weekly Clinical Observations.............................................................. Body Weights......................................................................................... Functional Observational Battery..................................................... Grip Performance................................................................................... Body and Tail Temperatures.............................................................. Flash Evoked Potentials (FEPs).......................................................... Cortical Flicker Response (CFR)........................................................ Auditory Brainstem Responses (ABRs)............................................. Somatosensory Evoked Potentials (SEPs).......................................... Caudal Nerve Action Potentials (CNAPs)....................................... Outliers.................................................................................................... Animals Removed From Study........................................................ Gross Pathology.................................................................................... Microscopic Pathology......................................................................... 26 26 26 26 26 27 27 27 28 28 29 29 29 29 29 30 30 DISCUSSION................................................................................................ ACKNOWLEDGEMENTS.......................................................................... REFERENCES............................................................................................... 30 39 40 030713 SL 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 1 of 3, Page: 6 TABLE OF CONTENTS, continued SUMMARY DATA: TABLES and FIGURES Chamber Exposure Cone, Temp. & Humidity (Table 5)............... Inlife Observations Summary (Tables 6, 7)..................................... Body Weights (Statistics, Tables 8, 9)................................................ Functional Observation Battery (Tables 10,11)............................... Grip Performance (Fig. 1,2; Tables 12-18)........................................ Body Temperatures (Tables 19, 20).................................................... Flash Evoked Potentials (FEPs) (Fig. 3, 4; Tables 21-26).................. Cortical Flicker Response (CFR), (Tables 27, 28)............................ Auditory Brainstem Resp. (ABRs) (Fig. 5-10; Tables 29-34).......... Somatosensory Evoked Poten. (SEPs) (Fig. 11-14; Tables 35-42).... Caudal Nerve Action Poten. (CNAPs) (Fig. 15-18; Tables 43-46).. Outliers (Table 47)................................................................................ Spont. or Moribund Animals Submitted for Necr. (Table 48)..... Gross Pathology (Tables 49,50)........................................................... Brain Weights (Table 51)..................................................................... Histopathology (Tables 52,53)............................................................ VOLUME 2. In-life Data APPENDIX A, Water Analyses................................................................ APPENDIX B, Chamber Analyses.......................................................... INDIVIDUAL ANIMAL DATA APPENDIX C, Clinical Observations..................................................... APPENDIX D, Body Weight Data........................................................... APPENDIX E, Functional Observational Battery................................. APPENDIX F, Grip Performance Data.................................................... APPENDIX G, Temperature Data............................................................ APPENDIX H, FEP Data............................................................................ APPENDIX I, SEP-C Data.......................................................................... APPENDIX J, SEP-S Data.......................................................................... APPENDIX K, ABRc Data......................................................................... APPENDIX L, ABRio Data....................................................................... APPENDIX M, ABR30 Data...................................................................... APPENDIX N, CNAP Data...................................................................... APPENDIX O, CFR Data........................................................................... VOLUME 3. Pathology Data APPENDIX P, Brain Weight Data.......................................................... APPENDIX Q, Gross Pathology............................................................... APPENDIX R, Neuropathology............................................................... PAGE 46 47 55 57 65 74 79 87 89 101 113 121 122 123 125 126 1 2 30 38 46 158 168 174 180 186 192 195 198 201 207 1 3 61 SL 03071A 1,1,1-T subchronic neurotoxicity SUMMARY The Dow Chemical Company Project ID: K-001716-091 Volume 1 of 3, Page: 7 Male and female Fischer 344 rats were exposed to 1,1,1-trichloroethane vapors for 6 hrs/day, 5 days/wk, for 13 weeks. Exposure levels were 200 ppm, 630 ppm and 2000 ppm. Rats were clinically examined regularly and were given a functional observational battery monthly (FOB, including forelimb and hindlimb grip performance testing). After 13 weeks of exposure, the functional integrity of the nervous system was evaluated by FOB and by visual, auditory, somatosensory and caudal nerve evoked potentials. After functional testing, a subgroup of rats had histopathologic examination of brain, spinal cord, peripheral nerves and limb muscles. No treatment related findings were discovered in any parameter except for a slightly smaller forelimb grip performance in the 2000-ppm exposure group. A subgroup of rats were examined for grip performance 6 weeks postexposure; forelimb grip performance was still smaller in 2000-ppm rats. Forelimb nerves and muscles from these rats then were examined histopathologically, and no treatment-related lesions found. No reason was discovered for this difference in forelimb grip performance. Forelimb grip performance normally decreases (habituates) over time as a function of experimental conditions, and it is speculated that the daily acutesedative effects of 2000 ppm 1,1,1-trichloroethane had an interaction with the habituation process. Other than a possible indicator of acute sedation, there was no recognized toxicologic significance to this difference in forelimb grip performance. Si* 030n5 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 1 of 3, Page: 8 CONSENT AGREEMENT A consent agreement between several companies represented by the Halogenated Solvents Industry Alliance and USEPA (Federal Register,Vol 54, No. 162,40 CFR Part 799.5000, August 23, 1989) required several studies be performed on rats to determine the neurotoxic potential of 1,1,1trichloroethane (1,1,1-T): -- Acute motor activity study (inhalation exposure, with motor activity to be evaluated promptly postexposure). Information to be used for dose setting for a subsequent subchronic neurotoxicity study. -- Acute neurophysiology study (data collected during inhalation exposure). Information to be used for dose-setting for a subsequent subchronic neurotoxicity study. -- Acute neurophysiology and functional observational battery (gavage treatment, tested at time of maximal effects). Information to be used for dose-setting for a subsequent developmental neurotoxicity study. -- Subchronic neurotoxicity study (inhalation exposure). -- Memory task development and validation. -- Developmental neurotoxicity study. The subchronic neurotoxicity test requirement is the subject of this report. The USEPA originally proposed that subchronic neurotoxicity be evaluated by Functional Observational Battery, Motor Activity, Schedule Controlled Operant Behavior, and Neuropathology. As a result of the negotiation process, the final test agreement required a subchronic neurotoxicity study that included a Functional Observational Battery, a Sensory Evoked Potential Battery, and Neuropathology. SL 030716 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 1 of 3, Page: 9 The emphasis on electrophysiologic techniques for the evaluation of 1,1,1-T was predicated on its general utility in animal neurotoxicology (Dyer, 1985; Rebert, 1983), its use to characterize the acute effects of 1,1,1-T (Albee et al., 1990a), toluene and dichloromethane (Rebert et al., 1989a,b,c), and our experience with this technology (Albee et al., 1987; Albee et al., 1990b; Mattsson and Albee, 1988; Mattsson et al., 1988; Mattsson et al., 1989a,b; Mattsson et al., 1990a,b). In addition, evoked potentials are used in psychiatric diagnostic differentiation (Shagass et al., 1988), are effective for early detection of neurotoxicity in humans, including solvent neurotoxicity (Seppalainen, 1988), and have readily detected persistent changes in evoked potentials in rats after subchronic toluene 'abuse' (Mattsson et al., 1990b). INTRODUCTION The dose-setting goal, as stated in the consent agreement protocols, was to select exposure concentrations based on acute nervous system effects and on existing literature concerning classical toxicity endpoints from subchronic and chronic studies. The highest dose selected was to have a clearly demonstrable neurobehavioral effect. The acute neurobehavioral effects, however, should not be of such severity as to cause persistent non-CNS health complications that will compromise the interpretability of the neurotoxicity data. This defines, in a sense, a neurofunctional maximum tolerated dose. The acute nervous system effects element of dose-setting was accomplished in the acute effects studies required by the test agreement. Acute neurophysiological effects were demonstrated in rats exposed to 2000 or 1000 ppm 1,1,1-T for 6 hrs/day. Large flash evoked potential and electroencephalogram (EEG) changes were apparent in rats exposed to 2000 ppm for 6 hrs, and minor somatosensory changes also occurred. Smaller changes in evoked potentials and EEG were apparent at 1000 ppm (Albee et al., 1990a). SL 030717 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 1 of 3, Page: 10 The systemic toxicity element of dose-setting was addressed by literature review. Repetitive exposure studies indicate altered neurochemistry at concentrations of 1000 ppm or less (Karlsson et al., 1987; Rosengren et al., 1985; Savolainen et al., 1977), the presence of mild hepatic and olfactory mucosal changes after subchronic exposure to 1500 to 2000 ppm (Quast et al., 1988; Calhoun et al., 1981), and developmental delay in pups of dams exposed to 2100 ppm (York et al., 1982). TEST PROCEDURES TEST SPECIES AND HUSBANDRY: Species: Fischer 344 rats were used in the study. Age: Approximately 16 weeks old. Sex: Equal numbers of male and nulliparous and nonpregnant female rats were put on study. Husbandry: The study rats were purchased from the Charles River Breeding Laboratory, Kingston, New York. This strain of rat was chosen because of its general use in toxicity testing, availability of historical data and a reliable commercial supplier. Shortly after arrival at the laboratory1 the animals were checked for health status by a veterinarian and determined to be in good health. Rats were housed one per cage in suspended stainless steel cages which have wire-mesh floors. The holding room was maintained at approximately 22 C, 50% humidity, and a 12-hour light-dark cycle. 1Fully accredited by the American Association for Accreditation of Laboratory Animal Care (AAALAC). SL 030718 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 1 of 3, Page: 11 Purina Certified Rodent Chow (#5002) and municipal drinking water were available ad libitum throughout the study except during exposure. Analysis of the Purina Certified Chow was performed by the Purina Mills Inc. to confirm that the diet provided adequate nutrition and to quantify the levels of selected contaminants. Analysis of the tap water was performed according to the Standard Operating Procedures of The Toxicology Research Laboratory (Appendix A). Analyses indicated that feed and water were within acceptable limits. Numbers: Fourteen per sex per group were put on study. The animals were stratified by weight and then randomly assigned to an exposure group using a computer program. Animals that were placed on study were identified by a uniquely numbered metal ear tag. Animals not placed on study were removed from the study room. A subsample of 24 (12 males and 12 females, factorial ANOVA) should be able to detect a one standard deviation change in the dependent variables with 90% power at a=0.05. Previous experience indicates the following coefficients of variation (CV) for the tests employed: Grip strength - 20%; Latency difference < 10%; Correlation * 10%; Power * 35%; Body weight < 5%; Temperature <5%. Controls: A concurrent air control was used. DOSE LEVELS AND DOSE SELECTION: A control and three levels of treatment were used. The USEPA and HSIA had a subchronic neurotoxicity dose-setting meeting on March 22,1990. Based on the presence of large acute pharmacologic changes in CNS function at 2000 ppm 1,1,1-T, and the findings of mild systemic toxicity at about 2000 ppm in previous repetitive dose studies, a subchronic top exposure level of 2000 ppm was selected. Lower exposures were selected at half log and log intervals. The intermediate exposure level was 630 ppm, and the low exposure level was 200 ppm. SL 030719 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 1 of 3, Page: 12 Duration: 13 weeks. Route: Rats were exposed to 1,1,1-T vapors for 6 hr/day, 5 days/week, for 13 weeks. TEST MATERIAL: 1,1,1-Trichloroethane, stabilized with 0.1% butylene oxide, lot number TA9003051, was obtained from The Dow Chemical Company. The identity of the test material was confirmed by gas chromatography with thermal conductivity detection (GC/TCD) and infrared spectroscopy (IR). The sample of 1,1,1-T was determined, by GC, to be 99.9% pure with no impurities 0.1% (Heimerl, 1990; Putzig, 1990). EXPOSURE CHAMBERS AND ATMOSPHERE GENERATION: Stainless steel and glass 4.1 m3 Rochester-type exposure chambers (square with pyramidal top and bottom) were used with the airflow maintained at 800 1/min. Vapor distribution was evaluated prior to animal exposures by comparing the concentration at 4 points within the animal breathing zone with the primary sampling line (reference point). The concentration at all points was within 8% of the reference. The minimum and maximum chamber temperature and the relative humidity were recorded at the end of each exposure period. Test atmospheres of 1,1,1-T were generated according to the method of Miller et al. (1980). 1,1,1-T was metered (Fluid Metering Inc., Oyster Bay, NV) at a uniform rate into a glass J-tube filled with 6-mm glass beads. Compressed air, heated with a flameless heat torch (Master Appliance Corp., Racine, WI) to the minimum extent necessary (max. 42C), passed through the J-tube to volatilize the test material prior to entering the main chamber air stream. The concentration of 1,1,1-T in the chamber was monitored 1-2 times per hour with a Miran 1A infrared spectrometer. Calibration was performed with 03072 SL 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 1 of 3, Page: 13 air standards prepared by dilution of measured volumes of 1,1,1-T with metered volumes of compressed air in Saran2 bags. The concentration of 1,1,1-T in the chamber was determined by interpolation from standards via a data acquisition and computation system (Camile2, The Dow Chemical Company, Midland, MI). The analytical system was evaluated with at least one 1,1,1-T standard prior to each day of exposure. The nominal concentration (ratio of amount of test material vaporized to the total amount of air through the chamber) was calculated daily for each chamber. DESIGN SUMMARY: The combined protocol includes the functional observational battery, grip performance, electrophysiology and neuropathology. Fourteen rats of each sex were randomly selected for each treatment group. Exposure duration was approximately 13 weeks. The work volume (implantation, testing, perfusion) required that the rats be divided into two subgroups that started one week apart. Each subgroup was counterbalanced for numbers of rats per dose level. Functional observational battery and grip testing were conducted on all rats before exposure and monthly thereafter. An electrophysiological test battery was conducted on 12/sex/group after 13 weeks of exposure. Ten rats (5 males and 5 females) per exposure level were necropsied after electrophysiological testing, and the remaining rats were held postexposure. Since forelimb grip performance was lower in 2000-ppm rats after 13 weeks of exposure, the postexposure rats were retested for grip performance (retested 7 weeks postexposure). Forelimb muscles and nerves of postexposure rats were examined histopathologically. Counterbalancing: Testing was counterbalanced across dose groups. Each animal was tested individually. BODY WEIGHTS: Body weights were taken at preexposure and weekly during exposure. 2 Registered Trademark of The Dow Chemical Company. 030721 SL 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 1 of 3, Page: 14 OBSERVATIONS: Daily Observations: Cage-side observations for general health status were conducted twice daily. Weekly Observations: Each rat was removed from its cage and physically examined for changes in general health, which included specific observations on respiration, muscle tone, movement, tremor, skin and haircoat condition, salivation, lachmation, urine staining and fecal staining. Monthly Observations: A Functional Observational Battery (FOB, Table 1) was conducted on all rats of both subgroups at preexposure and monthly thereafter. The evaluation was done before daily treatment. All animals were carefully examined by the same trained technician. The handler presented the rats to the observer in a random order and in such a way that the observer did not know the identity of the rat nor the exposure level. Observations noted: (a) any unusual responses with respect to body position, activity level, coordination of movement, and gait; (b) any unusual or bizarre behavior including but not limited to headflicking, head searching, compulsive biting or licking, selfmutilation, circling, and walking backwards; (c) the presence of convulsions, tremors, increased levels of lacrimation and/or red-colored tears, increased levels of salivation, piloerection, pupillary dilation or constriction, unusual respiration (shallow, labored, dyspneic, gasping, and retching) and/or mouth breathing, diarrhea, excessive or diminished urination, vocalization; (d) forelimb and hindlimb grip peformance; (e) sensory function (audition, pain perception; visual placing not done since flash evoked potentials were accomplished and visual placing is a questionable response in albino rats). 030722 SI* 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 1 of 3, Page: 15 Table 1 - Functional Observational Battery (Methods): Hand-Held Observations Recorded As Pupil Size Respiration (increased, wheezing, etc.) Movement (bizarre behavior, vocalization tremors, convulsions, etc.) Skin and Haircoat (poor grooming, piloerection, etc.) Salivation Lacrimation Urine Staining Fecal Staining N, I, or D N or A N or A N or A N or I N or I + or + or - Observations in 50 cm x 50 cm clear plastic box: Locomotor Behavior (gait, pattern, activity level, coordination, etc.) Responsiveness to Touch Responsiveness to Sharp Noise Responsiveness to Tail Pinch N or A N, I, or D N, I, or D N, I, or D N = normal; A = abnormal, accompanied with description; I * increased; D = decreased; + = present; - = absent SL 030723 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 1 of 3, Page: 16 Grip Performance: Grip performance was tested at the same time as the FOB according to the procedure described by Mattsson et al. (1986). Briefly, the rats were selected in a random manner and given to the observer in such a way that the observer did not know the treatment status of the animal. The observer then placed the rat's forelegs on a bench and the hindfeet were set on a horizontal screen attached to a strain gauge. The observer then smoothly but firmly pulled backward on the tail until the rat's grip on the screen was broken. The strain gauge recorded the rat's resistance to the pull in grams. The strongest response of three trials was used for statistical analysis. Forelimb grip performance was tested similarly. In this application, the bench was removed from the apparatus, and the rats were placed so that the forefeet were on the screen and the hindfeet on the smooth horizontal plastic surface. The test sequence was the same as for hindlimb testing. SURGERY: Surgical implantation of epidural electrodes was performed 10 weeks into the study. Surgery was completed in counterbalanced order. Surgery for each half of the study was accomplished in five days. Anesthesia was induced by methoxyflurane and maintained with isoflurane. Once an animal was anesthetized, the eyes were treated with an ophthalmic ointment and the eyelids were closed with skin clips to prevent comeal drying and retinal damage by the lights. Once placed in the stereotaxic instrument (#900, David Kopf Instruments, Tujunga, CA), the rat had a rectal thermistor inserted 7 cm into the rectum to monitor core temperature during surgery. Body temperature was supported with a warm water blanket. Epidural electrodes (7-mm long, #0-80, stainless-steel set screws) were inserted into the skull and supported with dental acrylic. The top 3 mm of the set screws remained exposed above the acrylic so recording leads could be directly attached to the screws (an electrical plug was not used). The somatosensory electrode was placed 2.0 mm posterior and 2.0 mm lateral left of bregma, the visual cortex electrode was placed 1.5 mm anterior and 3.0 mm lateral right of lambda, an~ the cerebellar electrode was located 3.0 mm posterior and 0.0 mm 0307*'' 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 1 of 3, Page: 17 lateral of lambda. A reference electrode was placed 8.0 mm anterior and 1.0 mm lateral left of bregma. POSTEXPOSURE TESTS: The following data were collected beginning 65 or more hours after 13 weeks of exposure: functional observational battery (FOB), grip performance, flash evoked potential (FEP), cortical flicker response (CFR), auditory brainstem response to clicks (ABRc), auditory brainstem response to tone pips at 10 kHz (ABR]q) and 30 kHz (ABR30), somatosensory evoked potentials recorded from the sensory cortex (SEP-S) and from the cerebellum (SEP-C), and caudal nerve action potentials to single stimulus (CNAPi) and paired stimuli (CNAP2). Rats were physically restrained during electrophysiological testing, which took about 35 min for the test battery. Electrophysiological System: The electrophysiological system was a Nicolet Pathfinder II (Nicolet Biomedical Instruments, Madison, WI). Data sweeps (msec segments of EEG) were digitally sampled 512 times and averaged by an online computer. Rectal temperature was recorded prior to each electrophysiological test except the caudal nerve test, when tail temperatures were taken. The physical parameters for the different evoked potential tests are listed in Table 2. Rats were physically restrained during electrophysiological testing. Several tests were conducted: (1) Flash Evoked Potential (FEP, medium intensity) was recorded from the visual cortex (FEP-V) and from the cerebellum (FEP-C). The FEP-C was considered ancillary data only, was qualitatively evaluated, and was placed in the study file. (2) Cortical Flicker Response (CFR): A CFR, at a flash rate of 48 flashes/second, was collected. A simultaneous plus-minus average was recorded to provide a reference or baseline (noise) to which the signal (normal average) was compared. The variance of the voltage 030725 SL 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 1 of 3, Page: 18 across each response was determined and the signal variance to noise variance ratio was calculated. (3) Auditory Brainstem Response to Clicks (ABRc). Table 2: Physical parameters of eiectrophysiological tests. Amplifier Sweep Test Stimulus Stimulus Stimulus Filters Duration Intensity Rate/sec (Hz) (msec) FEP* Flash 13 cd-s/m2 0.7 03-500 150 & 750 CFR Flash 13 cd-s/m2 48 03-500 25 Number of Sweeps 200 200 ABRct Click 75 dB linear 29.1 100 - 3000 ABRlOt 10 kHz tone 70 dB linear 29.1 100 - 3000 ABR30t 30 kHz tone 80 dB linear 29.1 100 - 3000 12 12 12 2,000 2,000 2,000 SEP CNAP Electrical Electrical 3 mA, 50 (lsec 3 mA, 20 Msec 1.1 10.1 1-1500 1-3000 35 & 150 20 200 200 * Calibrated with a United Detector Technology 350 photodetector (plus 111 filter and lumilens 1153) placed in the same position as the rat, facing a white reflective surface (cubicle wall) opposite the strobe. t The distance from speaker to ears was about 17 cm. Sound pressure level calibrated on linear scale with a Bruel & Kjaer Model 2230 with 1/4 inch condenser microphone model 4135 placed in the restrainer at the location of the ears (rat removed). (4) Auditory Brainstem Response to Tone Pips (ABRjo and ABR30): Auditory brainstem responses to tone pips were tested at middle and high frequencies (10 kHz and 30 kHz). Each tone pip had a 2.25-msec rise/fall ramp and no plateau (4.5-msec total duration). (5) Somatosensory Evoked Potentials (SEP-S and SEP-C): Ventrolateral caudal nerves were stimulated at the base of the tail and a response was recorded at the somatosensory cortex (SEP-S) and from the cerebellum SL 030726 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 1 of 3, Page: 19 (SEP-C). The stimulating electrodes were small needles set into the bottom of a plastic tray that fit the tail (Rebert, 1983). (6) Caudal Nerve Action Potentials (CNAP): Ventrolateral caudal nerves were stimulated near the tip of the tail and mixed nerve action potentials from single stimuli (CNAP}) were recorded at the base of the tail (Rebert, 1983). The stimulating and recording electrodes (separated by 9 cm) were mounted in a plastic tray. Subsequently, the nerves were stimulated with a pair of pulses (CNAP2 with interstimulus interval of 3 msec). WAVEFORM ANALYSES: Digital Filtering: A computer routine (Nicolet Biomedical Instruments, Madison, WI) digitally filters each of the waveforms that were collected with a broad-band analog filter. Digital filter settings are as follows: FEP 1-250 Hz; SEP-S and SEP-C with a 35-msec data sweep filtered at 85-750 Hz; SEP-S and SEP-C recorded with a 150-msec data sweep filtered at 1-500 Hz. The early components of the SEP were high-pass filtered above 85 Hz to cause the computerized cross-correlation/latency calculations to emphasize the higher frequency components. This high-pass filtration causes the computer to simulate more accurately human waveform evaluations, which emphasize the details contained in the higher frequency components. Waveform Illustrations: Figures were prepared of group composites, by sex, of all evoked potentials at all dose levels. Quantitative Analyses: Evoked potential waveforms were collected from all test animals, and individual and group composite waveforms visually examined. Waveforms from all treatment groups and control animals were statistically evaluated. 030727 SL 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716*091 Volume 1 of 3, Page: 20 An automated computer technique analyzed the FEP-V, SHP-S, SEP-C, ABR^ABRjq, ABR30 and CNAP single and paired pulse data by quantification of differences from a template in waveform shape, latency and power. A template for each type of response was created by making a 'grand average' of all the control records for each sex. A window (starting and ending point in msec) was established for each type of response. The window widths for automated computer scoring were: FEP = 35-95.1 msec, FEP 94.5-280, ABR (click) = 1.0-5.0 msec, ABR10 = 1.5-5.9 msec, ABR30 = 1.5-5.5 msec, SEP-C = 4-18 and 18-70 msec, SEP-S 4.0-16.5 and 16.5-90.0 msec, CNAP (single stimulus) = 2.8-7.4 msec, CNAP (paired stimuli) = 6.0-10.0 msec. The computerized analysis included optimal correlation of an individual waveform to the template waveform, the latency difference (phase shift in msec required to reach optimal correlation), and total power in the window (RMS volts). Subsequent to computerized waveform evaluation, the computers' measurements of latency difference for each waveform for each animal were visually confirmed by an experienced auditor having no knowledge of animal identity or treatment When the computerized analysis was determined to be in error (usually due to mismatching of peaks), the auditor's value was accepted and a new cross-correlation coefficient was calculated. An alternative to power analysis was conducted on the N3 component of the FEP waveform. The baseline-to-peak amplitude for the N3 component was determined and statistically analyzed. Statistical Analyses: Electrophysiology data (optimal correlation, latency difference and power) were analyzed by a factorial multivariate analysis of variance (MANOVA), using the general linear model (GLM) procedure of SAS (SAS Institute Incorporated, Cary, NC). The factors were treatment, sex and group. The main effects of treatment, sex, group, and interactions of sexby-treatment and group-by-treatment were assessed by an F-test based on the 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 1 of 3, Page: 21 Hotelling-Lawley trace statistics. Multivariate contrasts of each treatment level versus control were conducted using the overall variance/covariance structure (pooled variance). Body weight and body temperature were used as covariates for electrophysiological measures. The statistical procedure also provided analogous univariate analyses of variance (ANOVA) for the 3 dependent variables of the MANOVA: optimal correlation, latency difference and power. The series of temperatures collected during electrophysiological testing were analyzed by factorial MANOVA/ANOVA. Grip performance data (forelimb and hindlimb) were analyzed by factorial MANOVA/ANOVA, and body weight was used as a covariate. Body weight was analyzed by factorial repeated-measure ANOVA (rep-ANOVA; preexposure, 1 mo., 2 mo., and 3 mo.). Brain weights and cortical flicker response (CFR) were tested with the factorial ANOVA. Because correlation values are often high (0.8 to 1.0) and, therefore, not well distributed for parametric analysis, the r values were arcsine transformed before statistical analysis. On the other hand, post hoc transformations were not conducted since they would weaken the integrity of the probability values derived from such data (Muller et al., 1984). Each variable was tested for homogeneity of variance by the F-max test (Bruning and Kintz, 1977). If heterogeneity of variance occurred and was attributable to one or two outlying data points, then these data were removed from parametric analysis and the data from these animals were discussed separately. Alpha adjustment for multiple statistical tests: This was an exploratory study in which numerous measurements were statistically compared in the same group of animals (Table 3). For this reason, a modest alpha correction was made to adjust for repetitive testing. Probability values, however, are reported uncorrected. SL 030729 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 1 of 3, Page: 22 Table 3- List of Tests that Require Statistical Analysis TESTS Body weight (weekly, test days) Grip performance (4x) TEST STATISTIC rep-ANOVA (T,S,G) MANOVA (T,S,G)/2 ANOVAs Flash Evoked Potential Early latency (3 var) Long latency (3 var) Cortical Flicker Response Auditory Brainstem Response Click (3 var) 10 kHz (3 var) 30 kHz (3 var) Somatosensory Evoked Potential Sensory Cortex Early latency (3 var) Long latency (3 var) Cerebellar Cortex Early latency (3 var) Long latency (3 var) Caudal Nerve Action Potential Single pulse (3 var) Second of pair (3 var) Temperatures during electrophysiology (6 times) Brain weight MANOVA (T,S,G)/3 ANOVAs MANOVA <T,S,G)/3 ANOVAs ANOVA (T,S,G) MANOVA (T,$,G)/3 ANOVAs MANOVA (T,S,G)/3 ANOVAs MANOVA (T,S,G)/3 ANOVAs MANOVA (T,S,G)/3 ANOVAs MANOVA (T,S,G)/3 ANOVAs MANOVA (T,S,G)/3 ANOVAs MANOVA (T,S,G)/3 ANOVAs MANOVA (T,S,G)/3 ANOVAs MANOVA (T,S,G)/3 ANOVAs MANOVA (T,S,G)/6 ANOVAs ANOVA (T,S) (T,S,G) are factors: T=treatment, S=sex, G=group Body weight and body temperature were used as covariates for evoked potential analyses, and body weight was used as a covariate for grip performance and brain weight analyses. If one were to consider only the first level of statistical analysis (one overall ANOVA or MANOVA for each test), and not consider the statistical analyses of different doses vs control nor the individual variables within each MANOVA, then there were about 17 primary statistical analyses. A 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 1 of 3, Page: 23 Bonferroni correction would lead to a corrected alpha of 0.05 + 17 = 0.0029. Obviously, little statistical power would remain if one used a Bonferroni correction. Tukey et al. (1985) recommended a more modest correction, wherein alpha (0.05) is divided by the square root of the number of variables in a group of tests. If, to be more conservative, alpha were divided by the square root of the number of tests rather than the number of variables, then test alpha would equal 0.05 + Vl7 = 0.0121. To be more conservative yet, and remain in general accord to the alpha correction concepts of Tukey et al. (1985), a test level alpha of 0.02 was selected, and variables within MANOVAs have an alpha of 0.01. Tests were body weights, grip performance, and the specific electrophysiologic tests listed in Table 3. The final interpretation of the numerical data considered statistical analyses along with other factors such as dose*response relationships, waveform shapes, and whether the results were meaningful in light of other biologic findings. NEUROPATHOLOGY: After completion of 90 days on study, 10 rats per group (5 rats of each sex) were randomly selected for neuropathology. Animals were perfused in situ by the following method. Rats were heparinized 10 minutes before perfusion with 0.2 ml (10000 USP/ml) per kg body weight followed by methoxyflurane anesthesia. While under anesthesia, the rats were perfused with 0.05M phosphate buffer followed by a phosphate-buffered (O.SM/ solution of 13% glutaraldehyde - 4% formaldehyde. The brains of perfused rats were weighed. Tissues (Table 4) were collected and stored in phosphate buffered glutaraldehyde/ formaldehyde solution. SL 030731 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume \ of 3, Page: 24 Gross pathological examination by a veterinary pathologist was as thorough as possible for perfused tissues. Animals that dislodged implants were euthanatized and necropsied, but were not perfused. Because of forelimb grip performance findings, of the rats held 7 weeks postexposure, 10 control and 10 high concentration rats (5 rats of each sex) were randomly selected for necropsy, perfusion fixation and histopathologic examination of forelimb nerves (median and digital) and muscles* Five rats of each sex from intermediate exposure levels were necropsied and immmersion fixed. No tissues were examined in these postexposure intermediate exposure level rats. The following tissues, from the control and high concentration groups, were examined with light microscopy: forebrain, center of cerebrum, midbrain, cerebellum and pons, and medulla oblongata, Gasserian ganglion, spinal cord (cervical and lumbar enlargements), dorsal and ventral roots (C3-C6 and LjL4; at least 2 from each region), dorsal root ganglia (C3-C5 and LJ-L4: at least 2 from each region), peripheral nerves [sciatic, tibial, sural and digital (recovery only)] and eyes with optic nerves. Forelimb muscles (deep digital flexor), and median and digital nerves were examined in control and high concentration groups held 7 weeks postexposure. Tissues from animals exposed to lower concentrations were not examined because of a lack of treatment-related histologic findings at the top exposure level. Tissue were sectioned and routinely stained with hematoxylin and eosin (H & E). Because no treatment-related lesions were seen, special stains (Table 4) for myelin (LFB-PAS), axons (silver), and neurons (cresyl echt violet) were performed on nervous system tissue. 0307^2 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 1 of 3, Page: 25 Table 4. Tissue List and Stains Stains General5 Myelin^ Axonsc Cell bodies^ Forebrain Center of Cerebrum Cerebellum and Pons Midbrain Medulla oblongata + ++ + ++ + ++ + ++ + ++ Spinal cord (cross section and tangential) Cervical Swelling (C3-C6) + ++ Lumbar Swelling (L1-L4) + ++ Dorsal Root Ganglia (C3-Cg);(L]-L4) + ++ Dorsal and Ventral Root (C3"C6);(L|-L4) + ++ Gasserian ganglia + ++ Proximal sciatic nerve* + ++ Tibial nerve* + ++ Eyes + Optic nerve Sural nerve + + ++ Median nerve (recovery only) + ++ Digital (recovery only) + ++ Caudal nerve (saved) Head (saved) Skeletal muscle (gastrocnemius) + + + + -t- + + + + + + + + + + procedure performed; - procedure not performed *Both legs saved but only right leg nerves collected. a Hematoxylin and eosin b Luxol fast blue - periodic add-Schiff c Silver d Cresyl echt violet SL 030733 1,1,1-T subchronic neurotoxicity RESULTS The Dow Chemical Company Project ID: K-001716-09I Volume 1 of 3, Page: 26 TEST ATMOSPHERE: The mean analytical concentrations of 1,1,1-T in the chambers during exposures were 0, 209, 620, and 2016 ppm for the targeted levels of 0, 200, 630 and 2000 ppm, respectively. Mean nominal concentrations were 224, 656 and 2207 ppm. The temperature and relative humidity in all chambers were comparable (Table 5). OVERALL PERSPECTIVE: Although evoked potentials were significantly altered during acute exposure to 1,1,1-T at 1000 and 2000 ppm, no persistent treatment-related differences in evoked potential waveforms were discovered after 13 weeks of 1,1,1-T exposure. Of specific interest in solvent studies was the lack of a persistent effect on cortically generated components of the waveforms. The only statistically significant finding was a smaller forelimb grip performance for 2000-ppm rats after 13 weeks of exposure and again 7 weeks postexposure. Although forelimb grip performance was smaller in 2000-ppm rats, all groups, including controls, had a markedly increased forelimb grip performance at 7 weeks postexposure. The cause of these forelimb grip performance differences, and postexposure rebound, was not determined. No alterations in hindlimb grip performance occurred. No alterations were noted in electrophysiology of peripheral nerves, spinal cord or brain. No alterations were noted in the weekly clinical examinations or in the monthly functional observational batteries. No treatment-related lesions were noted in brain, spinal cord, peripheral nerves (including forelimb digital nerves), or limb muscles. WEEKLY CLINICAL OBSERVATIONS: Rats remained in very good health throughout the study, and there were no treatment-related clinical observations (Tables 6 and 7). BODY WEIGHTS: Body weights were virtually unaffected by treatment SL 030734 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 1 of 3, Page: 27 (Tables 8 and 9). Top exposure level males weighed 3% more than controls, and top exposure level females weighed 1.5% more than controls. Although body weights were comparable between treated and control rats, body weight (independent of treatment) was a significant covariate in SEP-C (Table 40), and for preexposure grip performance (Table 14). FUNCTIONAL OBSERVATIONAL BATTERY: No treatment-related effects were seen on the FOB (Tables 10 and 11). No differences were noted in gait or posture, in muscle tone, or in hindlimb extensor thrust responses. Sensory responses were judged normal, as were overall activity and reactivity. GRIP PERFORMANCE: Hindlimb grip performance gradually increased (Figure 1; Table 12), while forelimb grip performance slightly decreased throughout exposure (Figure 2; Table 13). Forelimb grip performance showed a remarkable increase in all test groups 7 weeks postexposure. This forelimb 'rebound' effect was greatest in control female rats, but was apparent in all test groups. There were no significant treatment-related differences in hindlimb grip performance at any time in the study (Tables 14-18), but forelimb grip performance was 9% less in male and 20% less in female 2000-ppm rats after 13 weeks of treatment (Table 13). These differences were statistically significant (Table 17). Forelimb grip performance of 2000-ppm rats was less than controls again at 7 weeks postexposure (Table 13 and 18). There were no correlative findings in any of the other measures in evoked potentials, clinical observations, FOBs or pathology to explain the forelimb grip differences. BODY AND TAIL TEMPERATURES: Body temperatures were taken just before each electrophysiological test (Tables 19 and 20). No statistically significant differences were found. Body temperatures of male rats from the 2000-ppm group were slightly higher than those of controls (0.2 to 0.4C, SL 030735 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 1 of 3, Page: 28 depending on the test). Body temperatures from 2000-ppm females were approximately 0.1 less than controls. Tail temperatures of both male and female rats from the 2000-ppm group were only 0.1C different from controls. Although body temperatures were comparable between treated and control rats, temperature was a significant covariate (independent of treatment) on numerous evoked potential tests: FEP (Table 22); ABRs, both for clicks and tone-pips (Table 30, 32 and 34); SEP (Table 36). FLASH EVOKED POTENTIALS (FEPs): FEP-Vs from control and treated rats were very similar (Figure 3 and 4), although careful examination of 200-ppm female FEPs (Figure 4) reveals a faster waveform at 95 to 280 msec (peaks P3N3). Overall, this area of the 200-ppm female FEP is about 13 msec faster than controls (p = 0.006; Tables 23, 24). No other significant treatment-related findings occurred at any exposure level (Tables 21-24), there was no doseresponse relationship, and males did not respond similarly. Thus, there was no supporting data to conclude that the faster FEPs in 200-ppm females were a treatment-related effect. The baseline-to-peak amplitudes of N3 were somewhat larger in males than females (independent of treatment), but were very similar in amplitude across treatment groups. No statistically significant N3 amplitude differences were detected (Table 25 and 26). FEP-Cs (cerebellar lead) showed no qualitative differences between treatment groups, and FEP-C individual animal waveforms and group composite waveforms were placed in the study file. CORTICAL FLICKER RESPONSE (CFR): The cortical flicker response was very similar across all treatment groups (Table 27). No statistically significant differences were found (Table 28). 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 1 of 3, Page: 29 AUDITORY BRAINSTEM RESPONSES (ABRs): Neither visual examination nor statistical analyses of ABR^ ABR^q or ABR30 waveforms revealed any evidence of treatment (Figures 5-10; Tables 29-34). SOMATOSENSORY EVOKED POTENTIALS: Neither visual examination nor statistical analyses of SEP-Ss or SEP-Cs revealed any evidence of treatment (Figures 11-14; Tables 35-42). CAUDAL NERVE ACTION POTENTIALS: Neither visual examination nor statistical analyses of CNAPs (single or paired pulses) revealed any evidence of treatment-related effects (Figures 15-18; Tables 43-46). OUTLIERS: No treatment-related pattern was discerned in data from outliers, which are identified in Table 47. Overall, 14 values were outliers, one each from 14 different rats; controls had 3/14; 200 ppm had 3/14; 630 ppm had 4/14 and 2000 ppm had 4/14. Females had 8/14 and males had 6/14. Statistics in the tables are marked and footnoted when outliers had been removed. ANIMALS REMOVED FROM STUDY: Four rats per dose level, 16 rats total, were removed from the study for miscellaneous reasons (Table 48). One rat died of accidental trauma, one was found dead, one moribund, and one rat had no diagnostic classification. Four rats died from the anesthetic procedure. A methods change was instituted to insure that the tongue is clear of the airway when the head is in the stereotaxic apparatus. There were no subsequent anesthetic problems. Eight rats were removed from the study because of dislodgement of the electrode implants; five implants were lost during the exposure/testing portion of the study, and three were lost during the postexposure period. This rate of implant loss was unexpectedly high, and appeared to be due to a surgical technique change that diminished the bone-acrylic bond (the moisture content of bone at the acrylic interface was too high). This bonding problem has been solved. SL 030737 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 1 of 3, Page: 30 GROSS PATHOLOGY: None of the gross pathologic observations (Tables 49 and 50) were related to treatment with 1,1,1-T. Some gross observations were associated with the electrode implant or the implant surgical procedure. Other gross observations were incidental and represented background findings for F344 rats of a similar age. Brain weights collected at the end of 13 weeks of exposure were unaffected by treatment (Table 51). MICROSCOPIC PATHOLOGY: None of the histopathologic observations in rats exposed to 2000 ppm 1,1,1-T for 13 weeks were attributed to the test compound (Tables 52 and 53). Observations in control and treated animals were similar in incidence. Most observations represented background findings of F344 rats of similar age or were attributed to the electrode implant or the implant surgical procedure. DISCUSSION Rats in this subchronic inhalation neurotoxicity study were exposed to 1,1,1trichloroethane (1,1,1-T) at concentrations up to 2000 ppm, levels high enough to alter CNS function acutely (Kjellstrand et al., 1985; Albee et al., 1990a), and high enough to cause mild systemic toxicity (Quast et al., 1988; Calhoun et al., 1981). Although exposure of rats to 1000 ppm alters EEG and evoked potentials, the neurofunctional effects of lower 1,1,1-T exposures have not been carefully evaluated in experimental animals. Task performance of human beings, however, appeared to be affected during exposure to 350 ppm, and perhaps as low as 175 ppm (Mackay et al., 1987). In spite of subchronic exposure to 1,1,1-T at levels sufficient to alter acutely CNS function, no persistent changes were noted in evoked potentials. Of indirect relevance to questions of higher order CNS dysfunction (i.e., cognitive effects of solvent exposure) was the lack of treatment-related differences in flash evoked potentials, which are generated entirely in the cerebral cortex, and a lack of change in the longer latency components of the 0301 SL 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 1 of 3, Page: 31 somatosensory evoked potential, which also are generated in the cerebral cortex (Mattsson et al., 1991). Because of very complex cortical-cortical loops, and cortical-subcortical-cortical loops, evoked cortical activity lasts for up to hundreds of milliseconds. It is these longer latency potentials that are most affected by differences in attention, alertness, and many other psychological states and mental disorders (Shagass et al., 1986; Spehlmann, 1985). Thus, CNS conditions associated with psychiatric and cognitive dysfunction also cause alterations in evoked potentials, indirectly via altered attentional states, and directly via altered processing of sensory information. The lack of evoked potential effects raises a question of statistical and experimental power. Although statistical power for MANOVA is unknown (due to the complexity of the calculations), our coefficients of variation were reasonably small, and sample sizes reasonably large (see Results). Thus, reasonable statistical power was likely present. Experimental power is best demonstrated in other studies in our laboratory, which have detected psychophysiological, pharmacological, and pathological changes in evoked potentials. Transient changes in control rat evoked potentials have occurred after 6 hrs exposure to air, presumably from psychophysiological effects due to experimental conditions (Albee et al., 1990a). In the same study, different effects on evoked potentials were seen during exposure to 1,1,1-T (pharmacological effects). Mild diet restriction for one month, causing a 9% decrease in body weight compared to free fed controls, was associated with evoked potential differences that probably were psychologically associated rather than evidence of nutritional pathology (Albee et al., 1987). Subchronic exposure of rats to toluene in an 'abuse' paradigm (repeated, short, high-level exposures) caused dramatic slowing and disorganization of the flash evoked potential, severe flattening of high frequency auditory brainstem responses, and smaller effects on somatosensory evoked potentials (Mattsson et al., 1990b). These toluene effects occurred in the absence of neuropathologic changes (perfusion, extensive light microscopy). Subchronic SL 030739 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 1 of 3, Page: 32 exposure to 100 ppm sulfuryl fluoride, a level too low to create brain lesions, caused slowing of visual, auditory and somatosensory evoked potentials (Mattsson et al., 1988). A rat model of cretinism, caused by very mild congenital hypothyroidism, also demonstrated evoked potential changes (Albee et al., 1990b). Thus, the general experimental design used in this 1,1,1-T study can detect CNS changes from many different etiologies. Although no changes in evoked potentials were detected in this subchronic 1,1,1-T study, rats exposed to 2000 ppm 1,1,1-T had a decreased forelimb grip performance (Table 15). Hindlimb grip performance and virtually all other neurofunctional and neuromorphologic measures were within normal limits. In the absence of correlative information, the meaning of the decreased forelimb grip performance was obscure. Consequently, several potential interpretations of decreased forelimb grip performance will be discussed: (1) A statistically false positive finding (unlikely); (2) An indicator of systemic toxicity (considered unlikely); (3) An indicator of neuropathy (extremely unlikely); (4) An indicator of myopathy (unlikely); (5) A natural but modified consequence of repetitive testing (most likely); (6) An effect of unknown origin (also correct). (1) Decreased forelimb grip performance as a statistically false positive finding: Control vs 2000 ppm forelimb grip differences had ANOVA p * 0.0004, females had an apparent dose-response relationship, and the results were substantially repeated 7 weeks postexposure. Although the grip differences were not large, it appears that they were real. (2) Decreased forelimb grip performance as an indicator of systemic toxicity: The top 1,1,1-T exposure level of 2000 ppm may have caused mild systemic toxicity as indicated by previous subchronic and chronic studies (Quast et al., 1988; Calhoun et al., 1981). Mild systemic toxicity is, however, an unlikely SV 030*7 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 1 of 3, Page: 33 cause of a differential effect only on foreiimb grip performance. Tilson et al. (1979) postulated that systemic toxicity should affect both forelimb and hindlimb function. This opinion was supported by their polybrominated biphenyl (PBB) study (Tilson et al., 1979). Rats were treated with PBB to cause systemic toxicity and PBB treated rats did have decreases in motor activity and decreased forelimb and hindlimb grip performance. (3) Decreased foreiimb grip performance as an indicator of neuropathy: This 1,1,1-T neurotoxicity study had a large number of clinical observations, evoked potential measures, and nervous system histologic evaluations that converge on the question of neuropathy (Figure 19). There was no convergence of findings supportive of a diagnosis of neuropathy, and it was concluded that the foreiimb grip differences were not the result of neuropathy. Central-peripheral distal axonopathy, by definition, affects the longest axonal pathways first. The longest axonal pathways are in the tail of rats, and in the hindlimbs and spinal cord dorsal column. Consistent with this model, neuropathy, as caused by CS2, acrylamide, 2,5-hexanedione and zinc pyridinethione all affect hindlimb grip performance before foreiimb grip performance (Tilson et al., 1979; O'Donoghue, 1989; Ross and Lawhom, 1990). Hindlimb grip performance was unaffected by subchronic exposure to 1,1,1-T (Table 15), and therefore was non-supportive of a diagnosis of distal axonopathy. The central component of central-peripheral distal axonopathy lies in the dorsal column of the spinal cord. Because of the length of the pathway, the distal extreme of the dorsal-column pathway, the area of the gradle nucleus, was particularly important in neurotoxicity evaluations (Arezzo et al., 1982). Gracile components of the somatosensory evoked potential (SEP) show the Si 3074j 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 1 of 3, Page: 34 Figure 19: Examination for Central-Peripheral Distal Neuropathy Records Stlm 2 Record 1 ^ SPINAL CORD Record HEAD TAIL NERVES V Evoked Potential (m) Muscle section Merre section Evoked Potentials somatosensory evoked potential peaks at proximal spinal cord and midbrain caudal nerve potentials from single and paired pulses Observations coordination and posture responsiveness of tail to light touch muscle tone extensor thrust reflex reaction to tail pinch grip performance Histopathology lower brain stem (nucleus gracilis) upper spinal cord lower spinal cord spinal roots and ganglia (cervical & lumbar) sciatic nerve tibial nerve (to calf muscles) sural nerve calf muscle median nerve (7 wks postexposure) forefoot digital nerves (7 wks post) forearm muscles (7 wks postexposure) initial dysfunction from acrylamide neuropathy, and SEP changes precede behavioral alterations and appear coincident with the earliest detectable morphological changes (Arezzo et al., 1982). For these reasons, the dorsal column and gradle area were evaluated in the 1,1,1-T study by SEPs and by histopathological examination. Exposure to 1,1,1-T had no effect on either the SEPs or dorsal column morphology (Tables 35, 36, 39, 40, 50; Figures 11-14). The peripheral components of central-peripheral distal axonopathy are the peripheral nerves, and the longest peripheral nerves are in the tail and 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 1 of 3, Page: 35 hindlimbs. For this reason, caudal nerve action potentials (CNAPs) were evaluated, and hindlimb nerves and muscle were histopathologically examined. CNAPs and hindlimb nerve and muscle morphology were not affected by 1,1,1-T (Tables 43-46, 50; Figures 15,16). Forelimb tissues are not routinely examined nor saved because of the affinity of distal axonopathy for the hindlimb. With the advent of forelimb grip testing, however, it is likely that forelimb tissues will, at the least, be saved in future neurotoxicity studies. In this 1,1,1-T study, the realization of forelimb grip differences occurred after the 13-week necropsy, and forelimb tissues were not saved at necropsy. The forelimb grip effect was present, however, in the rats kept 7 weeks postexposure, and forelimbs from those rats were examined carefully at necropsy and histopathologically. Muscle, median nerve, and digital nerves from the forelimbs were normal. Finally, neuropathies are characterized by a considerable amount of recovery postexposure (Tilson et al., 1979; Maurissen et al., 1983; Ross and Lawhom, 1990). The persistence of the forelimb grip effect for 7 wks postexposure, therefore, is nonsupportive of a diagnosis of neuropathy. (4) Decreased forelimb grip performance as an indicator of myopathy: Myopathy would be expected to alter clinical observations of muscle tone, posture and gait, to affect grip performance, and to cause morphologic muscle changes. Except for forelimb grip differences, none of these other events occurred. It is difficult to generate a hypothesis of myopathy affecting only the forelimb, and in the absence of any other measures indicative of myopathy, it was concluded that myopathy was not the cause of the forelimb grip effect. (5) Decreased forelimb grip performance as a natural but modified consequence of repetitive testing: Grip performance requires some degree of 'cooperation' by the rat (O'Donoghue, 1989), and therefore has both a behavioral element and a physiological element (integrity of nerve, muscle SL 030743 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 1 of 3, Page: 36 and digits). Grip performance is not placed under control of any obvious positive reinforcement. In fact, jarring a firm grip may negatively reinforce the rat's gripping behavior and decrease the probability of such behavior. Failure to grip may have less aversive properties. Thus, with repetitive testing (three valid trials per test per limb, plus a trial or two ruled invalid due to incorrect posture, repeated several times per study), the rat has an opportunity to develop a new behavioral pattern during the grip test. Alternatively, it is a common observation in longer duration studies that the rats get much easier to handle. Perhaps, therefore, rats simply develop an overall habituation to experimental manipulation and handling. Whether the rats simply habituate to the experiment, or alter their behavior specifically towards forelimb grip performance is not known, but grip-performance changes over time are different between forelimb and hindlimb. In control data from repetitive test studies, hindlimb grip performance tends to increase with growth. In contrast, forelimb grip performance tends to either increase more slowly than hindlimb performance, or actually to decrease. The result is a decrease in forelimb performance relative to hindlimb performance (Table 54). Data from several studies indicate, therefore, that a decrease in forelimb grip performance during a repetitive testing study is a normal event. An additional question in the 1,1,1-T study, however, is why the forelimb grip decrease was greater in the 2000 ppm group than in the other groups or controls. This is a moot point, for there is no clear answer. One can speculate that the sedative properties of 2000 ppm 1,1,1-T exposures altered the rats' interactions with their environment. Conceivably, the highexposure rats were less stressed than controls by experimental procedures (placement into chambers, no access to food or water, other chamber experiences, etc.), and became more habituated to experimental conditions, 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 1 of 3, Page: 37 including grip testing. That the overall experiment must have had some effect on behavior can be seen in the postexposure forelimb grip performances (Figure 2). Forelimb grip performance increased dramatically in all groups at the 7-week postexposure test. A comparable effect did not occur for hindlimb grip performance. Table 54: Rat forelimb:hindlimb grip ratio, preexposure and at end of treatment or end o : experiment. preexp end exp % deer. Duration # tests (1) CM -3.1 -2.6 16 - 5 wk 6 (2) (3) 1,1,1-T 1,1,1-T 1,1,1-T 1,1,1-T CM CM CM TM CF TF -2.0 - 1.4 - 1.4 - 1.3 - 1.8 - 1.6 - 1.3 -0.8 - 1.1 -0.9 - 1.2 - 1.0 35 42 21 31 33 38 - 10 wk - 13 wk - 13 wk - 13 wk -13 wk - 13 wk 22 9 4 4 4 4 CM=controI males; CF=control females; TM=2000 ppm males; TF200Q ppm females (1) Squibb et al., 1983; F344 males, apparatus of Meyer et al., 1979. Control hindlimb grip performance remained relatively constant over time, while forelimb grip slightly decreased. (2) Ross and Lawhom, 1990; F344 males, apparatus of Meyer et al., 1979. Control forelimb and hindlimb grip decreased dramatically with repetitive testing; a plateau grip performance, with forelimb less than hindlimb, was reached about 50 days into the study. (3> O'Donoghue, 1989; Male rats, strain unknown, apparatus of Mattsson et al., 1986. Control foreiimb grip was greater than hindlimb at -3 hr, 1 hr, 6 hr, 1 day, but hindlimb grip was somewhat greater than forelimb on days 7 and 14, and hindlimb grip was clearly greater than forelimb on days 30, 60 and 90. Both forelimb and hindlimb grip increased with age and body weight, forelimb less than hindlimb. It appears, therefore, that foreiimb grip performance habituates over time as a function of experimental conditions, and that the daily sedative effects of 2000 ppm 1,1,1-T may have had an interaction with the habituation process. SL 0307A5 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 1 of 3, Page: 38 (6) Decreased forelimb grip performance as an effect of unknown origin: Systemic toxicity, peripheral neuropathy, and myopathy were systematically excluded from the list of differential diagnoses, and a plausible hypothesis of altered habituation to experimental conditions was advanced. In the final analysis, however, the reason for a smaller forelimb grip performance in 2000 Ppm rats is unknown. As mentioned above, forelimb grip behavior depends on two factors, the physiological ability to grip the screen, and the probability of gripping it. No findings in this 1,1,1-T study point to a decreased ability of the rat, but there were indications that the 2000-ppm rats were less prone to grasp the screen. Since decreases in forelimb grip performance occur naturally in subchronic studies (Table 54), and the rat's gripping behavior is not placed under any positive contingencies of reinforcement, it would be difficult to construe that these differences in grip performance are adverse. It is possible, however, that a decrease in forelimb grip performance minimizes physical strain on the forelimbs and paws, as well as discomfort or injury to digital skin and toe nails. In conclusion, evoked potentials were unaffected in this 1,1,1-T study, and the only treatment related finding was a slightly smaller forelimb grip performance in the 2000-ppm exposure group. Forelimb grip performance normally decreases (habituates) over time as a function of experimental conditions, and it is speculated that the daily acute-sedative effects of 2000 ppm 1,1,1-T may have had an interaction with the habituation process. Other than a possible indicator of acute sedation, there was no recognized toxicologic significance to this difference in forelimb grip performance. 0301 ^ 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 1 of 3, Page: 39 Acknowledgements This 1,1,1-trichloroethane study was complicated and required a large amount of support. In particular, we wish to express our great appreciation to the animal care personnel, and to Lee Ann Pugh, Thomas G. Sanderson, and Deborah L. Winieckie for the neurohistology. 0307*7 SL 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 1 of 3, Page: 40 REFERENCES Albee, R. R-, J. L. Mattsson, B. L. Yano and L. W. Chang. Neurobehavioral effects of dietary restriction in rats. Neurotoxicol. Teratol. 9: 203-2X1,1987. Albee, R. R., J. L. Mattsson, M. J. Beekman and R. H. Reitz. Acute neurophysiologic effects of 1,1,1-trichloroethane in rats. R&D report. The Dow Chemical Company, 1990a. Albee, R. R., J. L. Mattsson, K. A. Johnson, H. D. Kirk and W. J. Breslin. Neurological consequences of congenital hypothyroidism in Fischer 344 rats. Neurotoxicol. Teratol. 11(2): 171-183,1990b. Arezzo, J. C., H. H. Schaumburg and P. S. Spencer. Structure and function of the somatosensory system: a neurotoxicological perspective. Environ. Health Perspect. 44:23-30,1982. Bruning, J. L. and B. L. Kintz . Computational Handbook of Statistics. Scott, Foresman and Co., Illinois, 1977. Cabe, P. A., H. A. Tilson, C. L. Mitchell and R. Dennis. A simple recording grip strength device. Pharmacol. Biochem. Behav. 8(1):101-102,1978. Calhoun, L. L., J. F. Quast, A. M. Schumann, D. J. Scheutz, R. E. Carreon and D. A. Dittenber. Chlorothene VG3: Preliminary studies to establish exposure concentrations for a chronic inhalation study with rats and mice. R&D report. The Dow Chemical Company, 1981. 3 Registered Trademark of The Dow Chemical Company 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 1 of 3, Page: 41 Dyer, R. S. The use of sensory evoked potentials in toxicology. Fundam. Appl. Toxicol. 5: 24-40,1985. Heimerl, J. L. Compositional analysis of 1,1,1-trichloroethane for toxicology testing. The Dow Chemical Company R&D report, 1990. Karlsson, J.-E., L. E. Rosengren, P. Kjellstrand and K. G. Haglid. Effects of lowdose inhalation of three chlorinated aliphatic organic solvents on deoxyribonucleic add in gerbil brain. Scand. J. Work Environ. Health 13: 453-458,1987. Kjellstrand, P., B. Holmquist, I. Jonsson, S. Romare and L. Mansson. Effects of organic solvents on motor activity in mice. Toxicol. 35: 35-46, 1985. Mackay, C. M., L. Campbell, A. M. Samuel, K. J. Alderman, C. Idzikowski, H. K. Wilson and D. Gompertz. Behavioral changes during exposure to 1,1,1trichloroethane: Time-course and relationship to blood solvent levels. Am. J. Indust. Medicine 11: 223-239,1987. Mattsson, J. L. and R. R. Albee. Sensory evoked potentials in neurotoxicology. Neurotoxicol. Teratol. 10: 435-443; 1988. Mattsson, J. L., R. R. Albee and D. L. Eisenbrandt. Neurological approach to neurotoxicological evaluation in laboratory animals. J. Am. Coll. Toxicol. 8(2): 271-286; 1989a. Mattsson, J. L., R. R. Albee and D. L. Eisenbrandt. Neurotoxicologic evaluation of rats after 13 weeks of inhalation exposure to dichloromethane or carbon monoxide. Pharmacol. Biochem. Behav. 36(3): 671-681,1990a. SL 030749 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 1 of 3, Page: 42 Mattsson, J. L., R. R. Albee, D. L. Eisenbrandt and L. W. Chang. Subchronic neurotoxicity in rats of the structural fumigant, sulfuryl fluoride. Neurotoxicol. Teratol. 10: 127-133; 1988. Mattsson, J. L., R. R. Albee and S. J. Gorzinski. Similarities of toluene and ocresol neuroexcitation in rats. Neurotoxicol. Teratol. ll(l):71-75; 1989b. Mattsson, J.L., W.K. Boyes and J.F. Ross. Incorporating evoked potentials into neurotoxicity test schemes. In: Neurotoxicology. H. Tllson and C. Mitchell, editors. Raven Press, New York, 1991. Mattsson, J. L., S. J. Gorzinski, R. R. Albee and M. A Zimmer. Evoked potential changes from 13 weeks of simulated toluene abuse in rats. Pharmacol. Biochem. Behav. 36(3): 683-689,1990b. Mattsson, J.L., K.A. Johnson and R.R. Albee. Lack of neuropathologic consequences of repeated dermal exposure to 2,4-dichloroacetic add in rats. Fundam. Appl. Toxicol. 6:175-181,1986. Maurissen, J. P. J., B. Weiss and C. Cox. Somatosensory thresholds in monkeys exposed to acrylamide. Toxicol. Appl. Pharmacol. 71: 266-279, 1983. Meyer, O. A., H. A. Tilson, W. C. Byrd and M. T. Riley. A method for the routine assessment of fore- and hindlimb grip strength of rats and mice. Neurobehav. Toxicol. 1(3): 233-236, 1979. Miller, R. R., R. L. Letts, W. J. Potts and M. J. McKenna. Improved methodology for generating controlled test atmospheres. Am. Indust. Hyg. Ass. 41:844-846,1980. SL 030750 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 1 of 3, Page: 43 Muller, K. E., C. N. Barton and V. A. Benignus. Recommendations for appropriate statistical practice in toxicologic experiments- Neurotoxicol. 5(2): 113-126,1984. O'Donoghue, J.L. Screen for neurotoxicity using a neurologically based examination and neuropathology. J. Am. Coll. Toxicol. 8(1):97-115,1989. Putzig, C. Analysis of 1,1,1-trichloroethane, Lot TA 9003051, by infrared spectroscopy. The Dow Chemical Company R&D Report, 1990 Quast, J. F., L. L. Calhoun and L. E. Frauson. 1,1,1-Trichloroethane Formulation: A chronic inhalation toxicity and oncogenicity study in Fischer 344 rats and B6C3F1 mice. Fundam. Appl. Toxicol. 11: 611-625, 1988. Rebert, C. S. Multisensory evoked potentials in experimental and applied neurotoxicology. Neurobehav. Toxicol. Teratol. 5: 659-671, 1983. Rebert, C. S., M. J. Matteucci, G. T. Pryor. Acute electrophysiologic effects of inhaled toluene on adult male Long-Evans rats. Pharmacol. Biochem. Behav. 33: 157-165,1989a. Rebert, C. S., M. J. Matteucci, G. T. Pryor. Multimodal effects of acute exposure to toluene evidenced by sensory-evoked potentials from Fischer-344 rats. Pharmacol. Biochem. Behav. 32: 757-768, 1989b. Rebert, C. S., M. J. Matteucci, G. T. Pryor. Acute effects of inhaled dichloromethane on the EEG and sensory-evoked potentials of Fischer-344 rats. Pharmacol. Biochem. Behav. 34(3): 619-629,1989c. Si 30?5j 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 1 of 3, Page: 44 Rosengren, L. E., A. Aurell, P. Kjellstrand, K. G. Haglid. Astrogliosis in the cerebral cortex of gerbils after long-term exposure to 1,1,1-trichloroethane. Scand. J. Work Environ. Health 11: 447-456, 1985. Ross, J. F. and G. T. Lawhom. ZPT-related distal axonopathy: behavioral and electrophysiologic correlates in rats. Neurotoxicol. Teratol. 12(2): 153-159, 1990. Savolainen, H. P. Pfaffli, M. Tengen and H. Vainio. Trichloroethylene and 1,1,1-trichloroethane effects on brain and liver after five days intermittent inhalation. Arch. Toxicol. 38: 229-237, 1977. Seppalainen, A. M. Neurophysiological approaches to the detection of early neurotoxicity in humans. CRC Crit. Rev. Toxicol. 18 (4): 245-298,1988. Shagass, C., R.C. Josiassen and R.A. Roemer (editors). Brain Electrical Potentials and Psychopathology. Elsevier, New York, 1986. Shagass, C., R. C. Josiassen, R. A. Roemer. Psychiatric diagnostic differentiations by evoked potential measures: results of a second series. Res. Comm. Psychol. Psychiat. Behav. 13: 43-75; 1988. Spehlmann, R. Evoked Potential Primer. Butterworth Publishers, Boston, 1985. Squibb, R. E., H. A. Tilson and C. L. Mitchell. Neurobehavioral assessment of 2,4-dichlorophenoxyacetic acid (2,4-0) in rats. Neurobehav. Toxicol. Teratol. 5(3): 331-335,1983. Tilson, H. A., C. L. Mitchell and P. A. Cabe. Screening for neurobehavioral toxicity: the need for and examples of validation of testing procedures. Neurobehav. Toxicol. l(Suppl 0:137-148, 1979. SL 030752 1,1/1 -T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 1 of 3, Page: 45 Tukey, J. W., J. L. Ciminera and J. F. Heyse. Testing the statistical certainty of a response to increasing doses of a drug. Biometrics 41, 295-301,1985. York, R. G., B. M. Sowry, L. Hastings and J. M. Manson. Evaluation of teratogenicity and neurotoxicity with maternal inhalation exposure to methyl chloroform. J. Toxicol. Environ. Health, 9: 251-266, 1982. SL 030753 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 1 of 3, Page: 46 TABLE 5: Chamber Exposure Concentrations, Temperature and Relative Humidity TARGET CONCENTRATION, ppm Number of Exposures Time-Weighted Average Analytical Concentration, ppma Range of Analytical Concentrations, ppm Nominal Concentration, ppma Range of Nominal Concentrations, ppm 0 200 630 68 68 68 2000 68 ND 209.0= 4.9 620.2= 18.4 2016.3+ 52.1 ND 190.1-219.9 526.3-6405 1744.6-2206.0 ND 224,4 4.4 656.3= 14.06 2207.2= 111.8 ND 205.6-233.1 592.2-699.0 1990 - 2622 Temperature, Ca MIN. MAX. Relative Humidity, %a 21.1 0.7 21.9 0.3 62.7= 6.1 21.2 0.7 22.1+ 0.5 52.8= 2.4 20.6= 0.5 21.7= 0.5 53.1= 2.2 20.9 0.5 21.9+ 0.6 51.4 2.0 ND * Not Determined. a Mean Standard Deviation for 68 values 0307^ SL 1,1,1-T subchronic neurotoxicity SEX; MALE SPECIES: RAT CONC: 0 PPM NUMBER OF ANIMALS OBSERVED EYES WITHIN NORMAL LIMITS FECES AND URINE WITHIN NORMAL LIMITS GENERAL WITHIN NORMAL LIMITS MASS/NODULE WITHIN NORMAL LIMITS MOVEMENT/BEHAVIOR WITHIN NORMAL LIMITS RESPIRATION WITHIN NORMAL LIMITS SKIN, FUR AND MUCOUS MEMBRANES WITHIN NORMAL LIMITS TABLE 6: Inlife Observations Summary!! - Cl iaicaJ Exam The Dow Chemical Company Project ID: K-001716-091 Volume 1 of 3, Page: 47 WEEK WEEK WEEK WEEK WEEK WEEK WEEK MEEK WEEK WEEK WEEK WEEK WEEK 001 002 003 004 QOS 006 001 008 009 010 Oil 012 013 14 14 14 14 14 14 1 4 14 14 1 3 1 i 1 3 1 3 14 14 14 14 1 4 1 4 1 4 1 4 14 > ! 1 1 } , 1 J 14 14 14 1 4 1 4 1 4 14 1 4 14 11 1 1 t ) 1 I 14 14 1 4 14 1 4 14 H 1 4 14 11 ! 1 1 1 1 3 14 14 14 14 1 4 1 4 14 1 4 14 11 1 1 1 1 l 3 14 14 14 14 1 4 1 4 14 14 14 11 1 t 1 3 l 3 14 14 14 14 14 14 14 14 14 1 3 1 1 1 1 1 3 14 14 14 1 4 14 14 14 1 4 14 1.1 1 3 1 1 1 3 0 DATA ARE THE NUMBER OF AH[MARS WITH THE SPECIFIED OBSERVATION RECORDED DURING SCHEDULED CLINICAL EXAMS, ALL OTHER OBSERVATIONS ARE LISTED ON THE INDIVIDUAL ANIMAL REPORTS. 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 1 of 3, Page: 48 TABLE 6, continued: Inlite Observations Summary? - Clinical Exam SEX: MALE SPECIES: RAT CONC; 200 PPM NUMBER OF ANIMALS OBSERVED EYES WITHIN NORMAL LIMITS FECES AND URINE WITHIN NORMAL LIMITS GENERAL WITHIN NORMAL LIMITS MASS/NODULE WITHIN NORMAL LIMITS MOVEMENT/BEHAVIOR WITHIN NORMAL LIMITS RESPIRATION WITHIN NORMAL LIMITS SKIN, FUR AND MUCOUS MEMBRANES WITHIN NORMAL LIMITS WEEK WEEK WEEK WEEK WEEK WEEK WiEK WEEK WEEK WEEK WEEK WE.EK WEEK 001 002 003 004 00b 006 007 OfHl 009 010 Oil 0)7 013 14 14 14 14 1 4 14 14 14 14 14 14 14 14 14 14 14 1 4 14 1 4 1 4 1 4 14 14 14 14 14 14 14 14 14 1 4 14 14 1 4 14 14 14 14 14 14 14 14 14 14 14 14 14 14 14 14 14 14 14 14 14 14 14 14 1 4 1 4 14 14 14 1 4 14 14 14 14 14 14 14 14 14 14 14 14 14 14 14 14 14 14 14 14 1 4 1 4 14 14 14 1 4 14 14 14 14 14 14 14 14 14 14 14 14 1 4 14 SL 030756 e DATA ARE THE NUMBER OP ANIMALS WITH THE SPECIFIED OBSERVATION RECORDED DURING CIIEDULED CLINICAL EXAMS, ALL OTHER OBSERVATIONS ARE LISTED ON THE INDIVIDUAL ANIMAL REPORTS. SL 030757 -T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 1 of 3, Page: 49 TABLE 6, continued: Inlife Observations Summary!! - Clinical Exam sex: MALE SPECIES: RAT CONC: 630 PPM NUMBER OF ANIMALS OBSERVED EVES WITHIN NORMAL LIMITS EXCESSIVE CHRQMODACRYORRHEA FECES AND URINE WITHIN NORMAL LIMITS GENERAL WITHIN NORMAL LIMITS MASS/NODULE WITHIN NORMAL LIMITS MOVEMENT/BEHAVIOR WITHIN NORMAL LIMITS RESPIRATION WITHIN NORMAL LIMITS SKIN, FUR AND MUCOUS MEMBRANES WITHIN NORMAL LIMITS WEEK MEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK 001 002 003 004 005 006 001 ooe 009 010 Oil 012 013 14 14 14 14 14 14 14 14 14 13 13 13 13 14 14 14 14 14 14 14 14 14 13 13 13 11 0000000000 00 14 14 14 14 14 14 14 14 14 13 13 13 1 3 14 14 14 14 14 14 14 14 14 13 13 13 13 14 14 14 14 14 14 14 14 14 13 13 13 1 3 14 14 14 14 14 14 14 14 14 1 3 1 3 13 13 14 14 14 14 14 14 14 14 14 13 13 13 13 14 14 14 14 14 14 14 14 14 13 13 13 13 0 DATA ARE THE NUMBER OF ANIMALS WITH THE SPECIFIED OBSERVATION RECORDED DURING SCHEDULED CLINICAL EXAMS, ALL OTHER OBSERVATIONS ARE LISTED ON THE INDIVIDUAL ANIMAL REPORTS. 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 1 of 3, Page: 50 TABLE 6, continued: Inlite Observations Summery0 - Clinical Exam SEX: MALE SPECIES: RAT conc: 2000 PPM NUMBER OP ANIMALS OBSERVED EYES WITHIN NORMAL LIHITS PECES AND URINE WITHIN NORMAL LIMITS GENERAL WITHIN NORMAL LIMITS MASS/NODULE HITH1N NORMAL LIMITS MOVEMENT/BEHAVIOR WITHIN NORMAL LIMITS RESPIRATION WITHIN NORMAL LIMITS SKIN, FUR AND MUCOUS MEMBRANES WITHIN NORMAL LIMITS PERINEAL SOILING WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK 001 002 003 004 005 006 001 ooa 009 010 Oil 012 013 14 14 14 14 14 14 14 14 13 13 13 13 13 14 14 14 14 14 14 14 14 13 1 3 13 13 13 14 14 14 14 14 14 14 14 13 13 13 13 13 14 14 14 14 14 14 14 14 13 13 13 13 13 14 14 14 14 14 14 14 14 13 13 13 13 1 3 14 14 14 14 14 14 14 14 1 3 13 13 1 3 1 3 14 14 14 14 14 14 14 14 1 3 13 1 3 13 13 14 14 12 12 12 12 12 14 13 13 12 13 13 002 2 22 2 000 1 00 SL 030758 0 DATA ARE THE NUMBER OP ANIMALS WITH THE SPECIFIED OBSERVATION RECORDED DURING SCHEDULED CLINICAL EXAMS, ALL OTHER OBSERVATIONS ARE LISTED ON THE INDIVIDUAL ANIMAL REPORTS. SL 030759 1,1,1 -T subchronic neurotoxicity SEX: FEMALE SPECIES: RAT CONC: 0 PPM NUMBER OF ANIMALS OBSERVED EYES WITHIN NORMAL LIMITS EXCESSIVE CHROMODACRYORRHEA EYELIDS CLOSED FECES AND URINE WITHIN NORMAL LIMITS GENERAL WITHIN NORMAL LIMITS MASS/NODULE WITHIN NORMAL LIMITS MOVEMENT/BEHAVIOR WITHIN NORMAL LIMITS RESPIRATION WITHIN NORMAL LIMITS SKIN, FUR AND MUCOUS MEMBRANES WITHIN NORMAL LIMITS TABLE 7: Inlife Observations SummaryS - Clinical Exam The Dow Chemical Company Project ID: K-001716-091 Volume 1 of 3, Page: 51 WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK WEEK 001 002 003 004 005 006 007 ooe 009 010 Oil 012 013 14 14 14 14 14 14 14 14 14 14 14 14 14 14 0 0 14 14 14 14 14 14 14 0 0 14 14 14 14 14 14 14 0 0 14 14 14 14 14 14 14 .0 0 14 14 14 14 14 14 14 0 0 14 14 14 14 14 14 14 0 0 14 14 14 14 14 14 14 0 0 14 14 14 14 14 14 14 0 0 14 14 14 14 14 14 14 0 0 14 14 14 14 14 14 14 0 0 14 14 14 14 14 14 14 0 0 14 14 14 14 14 14 13 1 0 14 14 14 14 14 14 13 1 1 14 14 14 14 14 14 0 DATA ARE THE NUMBER OF ANIMALS NITH THE SPECIFIED OBSERVATION RECORDEO DURING SCHEDULED CLINICAL EXAMS, ALL OTHER OBSERVATIONS ARE LISTED ON THE INDIVIDUAL ANIMAL REPORTS. 1,1,1-T subchronic neurotoxicity TABLE 7, continued; Inlife Observations Summary** - Clinical Exam The Dow Chemical Company Project ID: K-001716-09) Volume 1 of 3, Page; 52 SEX: FEMALE SPECIES: RAT CONC: 200 PPM NUMBER OF ANIMALS OBSERVED EYES WITHIN NORMAL LIMITS EYELIDS CLOSED FECES AND URINE WITHIN NORMAL LIMITS GENERAL WITHIN NORMAL LIMITS HASS/NODULE WITHIN NORMAL LIMITS HOVEMENT/BE1IAVIOR WITHIN NORMAL LIMITS RESPIRATION WITHIN NORMAL LIMITS SKIN, FUR AND MUCOUS MEMBRANES WITHIN NORMAL LIMITS WEEK WEEK WEEK WEEK wh:k WKfh 001 00? 003 004 Ot)t> 00ft Oil i WEEK WEEK WEEK WEI h wn k WEEK (ION 009 0)0 01 1 o| / 013 14 14 14 14 14 14 14 14 14 1 3 n 1 i 13 14 14 14 14 14 14 14 14 14 13 11 1 .i 12 0 0 0 0. 0 0 0 0 0 0 0 0 1 14 14 14 14 14 14 1 4 1 4 14 1 3 11 ) 1 13 14 14 14 14 14 14 14 1 4 14 1 3 1J 1 I 13 14 14 14 14 14 14 1 4 1 4 14 1 3 1 3 1J 13 14 14 14 14 14 14 1 4 14 14 1 3 11 ] 1 1 3 14 14 14 14 1 4 14 11 14 14 l 1 11 1 1 1 J 14 14 14 14 14 1 4 1 4 14 14 1 1 11 1 ) 13 S DATA ARE THE NUMBER OF AN IHALS WITH THE SPECIFIED OBSERVATION RECORDED DURING SCHEDULED CLINICAL EXAMS, ALL OTHER OBSERVATIONS ARE LISTED ON THE INDIVIDUAL ANIMAL REPORTS. 03076 1 SL 030761 1,1,1-T subchronic neurotoxicity TABLE 7, continued: Inlife Observations Summary? - Cl inical -Exam The Dow Chemical Company Project ID: K-001716-091 Volume 1 of 3, Page: 53 SEX: FEMALE SPECIES: RAT CONC: 630 PPM NUMBER OF ANIMALS OBSERVED EXES WITHIN NORMAL LIMITS EYELIDS SQUINTED FECES AND URINE WITHIN NOHMAL LIMITS GENERAL WITHIN NORMAL LIMITS MASS/NODULE WITHIN NORMAL LIMITS MOVEMENT/BE ItAVIOH WITHIN NORMAL LIMITS RESPIRATION HITHIN NORMAL LIMITS SKIN, FUR AND MUCOUS MEMBRANES WITHIN NORMAL LIMITS WEEK WEEK WEEK WEEK WEEK MEEK WEEK WEEK WEEK WEEK WEEK WE* K WEEK 001 002 00 3 004 005 006 007 008 009 010 on 012 013 14 14 14 14 14 1 4 1 1 14 14 14 14 14 14 14 14 14 14 14 14 1 4 14 14 1 3 ] 4 1 1 iA 0 0 0 o' 0 0 0 0 0 1 0 0 0 14 H 14 14 14 14 14 1 4 14 14 1 4 14 14 14 14 14 I 4 14 11 1 1 H 14 14 14 11 14 14 14 11 14 14 14 14 1 4 14 1 4 14 14 14 14 14 14 14 14 14 1 4 14 14 14 14 14 14 14 14 14 14 14 14 14 14 14 14 14 14 14 11 14 14 14 14 14 9 4 H 1 4 1 4 14 14 14 DATA ARE THE NUMBER OF ANIMALS Mil'll THE SPECIFIED OBSERVATION RECORDED DURING Si I] LOU LED CLINICAL EXAMS ALL OTHER OBSERVATIONS ARE. LISTED ON THE INDIVIDUAL ANIMAL REPORTS. 1,1,1-T subchronic neurotoxicity TABLE 7, continued: Inlire Observations Summary!* - Clinical Exam The Dow Chemical Company Project ID: K-001716-091 Volume 1 of 3, Page: 54 SEX: FEMALE SPECIES; RAT CONCi 2000 PPM NUMBER OF ANIMALS OBSERVED EYES WITHIN NORMAL LIMITS FECES AND URINE WITHIN NORMAL LIMITS GENERAL WITHIN NORMAL LIMITS MASS/NODULE WITHIN NORMAL LIMITS MOVEMENT/BEHAVIOR WITHIN NORMAL LIMITS RESPIRATION WITHIN NORMAL LIMITS SKIN, FUR AND MUCOUS MEMBRANE:; WITHIN NORMAL LIMITS WEEK WEEK WEEK WEEK WEEK WEEK KEEK WEEK WEEK WEEK W1 EK KEEK WEEK 001 002 003 004 005 006 007 000 009 010 0 1 1 01 2 0)3 14 14 14 14 14 14 14 14 14 12 12 12 12 14 14 14 1 4 1 4 14 14 14 1 4 12 12 ) 2 12 14 14 I 4 14 14 14 14 14 14 12 12 12 12 14 14 1 4 14 14 14 1 4 14 14 12 12 12 14 14 14 14 14 14 14 14 14 12 12 12 12 14 14 14 14 14 14 14 14 14 12 1 ) 12 12 14 14 14 14 14 14 1 4 14 14 12 12 12 14 H 14 14 14 14 14 14 14 12 12 12 12 0 DATA ABE THE NUMBED OF ANIMALS HITH THE SPECIFIED OBSERVATION RECORDED DURING SCHEDULE!) CLINICAL EXAMS ALL OTHER OBSERVATIONS ARE LISTED ON THE INDIVIDUAL ANIMAL REPORTS. 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 1 of 3, Page: 55 Table 8: Body Weights (grams). Descriptive Statistics Cone. Mal^. 0 ppm 200 ppm 630 ppm 2000 ppm Female, 0 ppm 200 ppm 630 ppm 2000 ppm Pre-Exp. Mean SD X Mean SD N Mean SD X Mean SD X 296.5 13.2 14 295.7 12.9 14 296.6 12.2 14 298.0 12.0 14 Mean SD N Mean SD N Mean SD X Mean SD X 167.5 3.5 14 167.6 3.2 14 167.3 3.6 14 167.4 3.0 14 Day 23 317.5 16.0 14 319.9 16.7 14 320.9 11.0 14 323.5 18.7 14 178.6 5.6 14 179.0 3.5 14 179.3 7.3 14 182.6 6.3 14 Dav51 328.8 23.1 14 337.9 21.1 14 341.9 17.3 14 339.9 20.7 14 186.4 4.7 14 186.5 4.1 14 186.2 7.4 14 190.5 6.3 14 Dav 93 Dav 128 335.7 21.7 13 339.8 29.9 14 337.3 25.1 13 346.2 25.6 13 356.8 21.1 7 364.6 27.2 7 375.9 11.7 6 351.3 39.0 6 191.5 8.0 14 189.8 4.3 13 193.3 8.5 14 194.4 7.1 12 196.1 7.9 7 1933 5.4 7 199.6 6.0 8 194.7 4.3 5 Sh 030763 1,1,1-T subchronic neurotoxicity Table 9: Body Weights, Statistical Analyses* The Dow Chemical Company Project ID: K-001716-091 Volume 1 of 3, Page: 56 Level Between Subjects Effects Treatment (T) Sex (S) Within Subjects Effects Time (t) tXT txs Repeated Measures ANOVA 0.376 0.0001 0.0001 0.530 0.0001 a Values are actual p values; highlighted values are ANOVA a < 0.01. SL 030764 1,1,1 -T subchronic neurotoxicity The Dow Chemical Company Study ID: K-0017lWl Volume 1 of 3, Page: 57 sex PPM NUMBER OF ANIMALS OBSERVED TABLE 10: FUNCTIONAL OBSERVATIONAL BATTERY DAY -04* HA LLS 0 200 630 14 14 14 2000 14 EYES (PUPIL SIZE, LACRIMATION, ETC. FECES AND URINE (DECREASED, DARK, MUCOID, REDDISH, ETC.) GENERAL (SALIVATION, OBESE, NASA), DISCHARGE, ETC.) MOVEMENT/BEHAVIOR (INCOORD., MUSC. TONE, AGGRESSIVE, ETC.) RESPIRATION (DEEP, NOI ST, SNEEZE, ETC.) SKIN, FUR AND MUCOUS MEMBRANES PAPULES,MUZZLE,RIGHT SENSORY RESPONSIVENESS(TOUCH, AUDITORY, NOCICEPTIVE, VISUAL) AUDITORY RESPONSIVENESS - DECREASED DISPOSITION (MISSING, MORI IIUNI), ETC. | CO 0 0 0 0 0 0 0 0 00 00 00 00 0 () 00 I0 00 0 0 0 0 0 I 0 0 030765 8 DATA ARE THE HUMBER OF ANIMALS WITH THE SPECIFIED OBSERVATION 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Study ID: K-001716-091 Volume 1 of 3, Page: 58 SEX PPM NUMBER OF ANIMALS OBSERVED TABLE 10, continued: FUNCTIONAL OBSERVATIONAL BATTERY MONTH le MALES 0 200 6.10 2000 14 14 14 14 EYES (PUPIL SIZE, LACR1MATION, ETC.) 00 0 0 FECES AND URINE (DECREASED, DARK, MUCOID, REDDISH, ETC.) 0000 GENERAL (SALIVATION, OBESE, NASAL DISCHARGE, ETC.) 0000 MOVEMENT/HEIIAVI OR (1NCOOKD., MUEC . TONE, AGGRESSIVE, ETC.I (1 0 0 0 RESPIRATION (DEEP, NOISY, SNEEZE, ETC.) RESPIRATION SLOW 0 0 iJ I SKIN, EUR AND MUCOUS HEMHKANES 00a0 SENSORY RESPONSIVENESS(TOUCH, AUDITORY, NOCICEPTIVE, VISUAL) AUDITORY RESPONSIVENESS - DECREASED l 1 00 DISPOSITION (MISSING, MORI BUND, ETC.) 00 00 CD r* a DATA ARE THE NUMBER OF ANIMALS WITH THE SPECIFIED OBSERVATION. 030766 SL 030767 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Study ID: K-001716-091 Volume 1 of 3, Page: 59 sex PPM NUMBER OF ANIMALS OBSERVED TABLE 10, coni.inueti: FUNCTIONAL OBSERVATIONAL BATTERY MONTH 2* MALES 0 2 0G 630 2000 14 M 14 14 EYES (PUPIL SIZE, LACKIMAT ION, ETC.) FECES AND URINE (DECREASED, DARK, MUCOID, REDDISH, ETC.) GENERAL (SALIVATION, OBESE, NASAL DISCHARGE, ETC.) MOVEMENT/BEHAVIOR (INCOORD., MUSC. TONE, AGGRESSIVE, ETC.) RESPIRATION (DEEP, NOISY, SNEEZE, ETC.) SKIN, FUR AND MUCOUS MEMBRANES SENSORY RESPONSIVENESSITOUCH, AUDITORY, NOCICEPTIVE, VISUAL) DISPOSITION (MISSING, MORIBUND, ETC.) 0 0 0 0 0 0 0 0 00 00 00 00 00 00 o0 (10 0 0 0 0 0 0 0 0 e DATA ARE THE NUMBER OF ANiMAl.S WJ'JTI THE SPECIFIED OBSERVATION. 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Study ID: K-0M716-O91 Volume I of 3, Page: 60 SEX PPM NUMBER OF ANIMALS OBSERVED TABLE 10, continued: FUNCTIONAL OBSERVATIONAL BATTERY MONTH 3* MALES 0 200 630 2000 13 M 13 13 EYES (PUPIL SIZE, LACRIMATION, ETC.) FECES AND URINE (DECREASED, DARK, MUCOID, REDDISH, ETC.) GENERAL (SALIVATION, OBESE, NASAL DISCHARGE, ETC.) EMACIATED MOVEMENT/IIEIIAV I OH (J NCI (OKI)., Mlls< . TONE, AGGRESSIVE, EIC.I RESPIRATION (DEEP, NOISY, SNEIV.E, ETC.) SKIN, FUR AND MUCOUS MEMBRANES SENSORY RESPONSIVENESS I TOUCH, AUDITORY, NOCICEPTIVE, VISUAL! DISPOSITION (MISSING, MORIBUND, ETC.) ANIMAL: 9OA3SI0 DOSE: 200 COMMENT: THIN, NOT EMACIATED 0 0 0 0 0 0 0 0 00 0o I0 I) I) 00 0 00 00 0 0 0 0 0 0 0 U1 r1 8 DATA ARE THE NUMBER OF ANIMALS HITH THE SPECIFIED OBSERVATION. 030768 SL 030769 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Study ID: K-001716-091 Volume 1 of 3, Page: 61 SEX PPM NUMBER OF ANIMALS OBSERVED TABLE 11: FUNCTIONAL OBSERVATIONAL BATTER* OAT -04* FEMALES 0 200 630 14 14 14 EYES (PUPIL SIZE, LACRIHATtON, ETC.1 000 FECES AND URINE (DECREASED, DARK, MUCOID, REDDISH, ETC.) 00a GENERAL (SALIVATION, OBESE, NASAL DISCHARGE, ETC.) 00o MOVEMENT/BEHAVIOR (INCOORD., MUSC. TONE, AGGRESSIVE, ETC.) 00o RESPIRATION (DEEP, NOISY, SNEEZE, He.) 00o SKIN, FUR AND MUCOUS MEMBRANES 00o 0 0 0SENSORY RESPONSIVENESS(TOUCH, AUDITORY, NOCICEPTIVE, VISUAL) DISPOSITION (MISSING, MORIBUND, ETt .1 000 2000 14 0 0 0 0 (I 0 0 0 8 DATA ARE THE NUMBER OF ANIMALS MITH THE SPECIFIED OBSERVATION. 1,1,1-T subchronic neurotoxicity SEX PPM NUMBER OF ANIMALS OBSERVED The Dow Chemical Company Study ID. K-00I7I6-09I Volume I of 3, Page: 62 TABLE 11, continued; FUNCTIONAL OBSERVATIONAL BATTERY MONTH 1* FEMALES 0 200 630 2000 14 M 14 14 EXES (PUPIL SIZE, LACRIMATION, ETC.) 00 00 FECES AND URINE (DECREASED, DARK, MUCOID, REDDISH, ETC.) 0000 GENERAL (SALIVATION, OBESE, NASAL DISCHARGE, ETC.) 00 00 MOVEMENT/BEHAVIOR (INCOORD., HUSC. TONE, AGGRESSIVE, ETC.) 0000 RESPIRATION (DEEP, NOISY, SNEEZI^, ETC.) 0000 SKIN, FUR AND MUCOUS MEMBRANES 00 o 0 SENSORY RESPONSIVENESS(TOUCH, AUDITORY, NOCICEPTIVE, VISUAL) AUDITORY RESPONSIVENESS - DECREASED 20 00 DISPOSITION (MISSING, MORIBUND, ETC.) 00 0 0 e DATA ARE THE NUMBER OF ANIMALS WITH THE SPECIFIED OBSERVATION. SL 030770 SL 030771 1,1,1 -T subchronic neurotoxicity The Dow Chemical Company Study ID: K-001716-091 Volume 1 of 3, Page: 63 SEX PPM NUMBER OF ANIMALS OBSERVED TABLE 11, continued: FUNCTIONAL OBSERVATIONAL BATTERY MONTH 2* FEMALES 0 200 630 2000 14 14 14 14 EYES (PUPIL SIZE, LACRIMATION, ETC.) 0 0 00 FECES AND URINE (DECREASED, DARK, MUCOID, REDDISH, ETC.) 0 0 00 GENERAL (SALIVATION, OBESE, NASAL DISCHARGE, ETC.) 0 0 00 MOVEMENT/BEHAVIOR (INCOORD., MUSC. TONE, AGGRESSIVE, ETC.) 0 0 00 RESPIRATION (DEEP, NOISY, SNEEZE, ETC.) 0 0 00 SKIN, FUR AND MUCOUS MEMBRANES 0 0 00 0 0 0 0SENSORY RESPONSIVENESS(TOUCH, AUDITORY, NOCICEPTIVE, VISUAL) DISPOSITION (MISSING, MORIBUND, ETC.) 0 0 00 e DATA ARE THE NUMBER OF ANIMALS HITH THE SPECIFIED OBSERVATION. 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Study ID: K-001716-091 Volume 1 of 3, Page: 64 SEX PPM NUMBER OF ANIMALS OBSERVED TABLE 11, continued: FUNCTIONAL OBSERVATIONAL BATTERY MONTH 3 FEMALES 0 200 630 2000 14 13 14 12 EYES (PUPIL SIZE, LACRIMATION, ETC.) EXCESSIVE CHROMOOACRYORRHEA, LEFT EXCESSIVE CHROMODACRYORRHEA,RIGHT EXCESSIVE CHROMODACRYORRHEA,BILATERAL FECES AND URINE (DECREASED, DARK, MUCOID, REDDISH, ETC.) GENERAL (SALIVATION, OBESE, NASAL DISCHARGE, ETC.) MOVEMENT/BEHAVIOR (INCOORD., MUSC. TONE, AGGRESSIVE, ETC.) AGGRESSIVE RESPIRATION (DEEP, NOISY, SNEEZE, ETC.) SKIN, FUR AND MUCOUS MEMBRANES SENSORY RESPONSIVENESS(TOUCH, AUDITORY, NOCICEPTIVE, VISUAL) AUDITORY RESPONSIVENESS - DECREASED DISPOSITION (MISSING, MORIBUND, ETC.) 1 0 10 0 1 00 0 1 00 0 0 00 0 0 00 0 0 10 0 0 00 0 0 00 0 0 10 0 0 00 SL 030772 0 DATA ARE THE NUMBER OF ANIMALS HITH THE SPECIFIED OBSERVATION 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 1 of 3, Page: 65 Figure 1: Hindlimb Grip Performance of Rats Exposed to 1,1,1Trichloroethane Vapors for 13 Weeks. Pre-Exp. One Two MONTH ca+ni 1100 ?& 900 <u c<0 700 -' s 3 500 dQ.J .9- 300 U U ioo: Female Hindlimb Three Post-Exp. 0 ppm SI 200 ppm 630 ppm 2000 ppm Pre-Exp. One Two Three Post-Exp. MONTH SL 030773 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 1 of 3, Page: 66 Figure 2: Forelimb Grip Performance of Rats Exposed to 1,1,1Trichloroethane Vapors for 13 Weeks. G rip Performance (gm) SE 1100 900 700 500 300 100 Pre-Exp. One Two Three Post-Exp. MONTH 0 ppm D 200 ppm G rip Performance (gm) SE * p 0.004 * p = 0.012 Pre-Exp. One Two Three Post-Exp. MONTH SL 03077A 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 1 of 3, Page: 67 Table 12: Hindlimb Grip Performance (g). Descriptive Statistics Cone. Male. 0 ppm 200 ppm 630 ppm 2000 ppm Female. 0 ppm 200 ppm 630 ppm 2000 ppm Pre-ExD. IMonth 2Month 3Month Post-Exp. Mean SD N Mean SD N Mean SD N Mean SD N 514.6 83.3 14 516.4 79.9 14 557.5 82.7 14 555.0 89.6 14 564.6 82.3 14 561.4 81.4 14 584.6 70.9 14 554.6 52.5 14 602.1 65.7 14 614.6 114.5 14 633.6 96.9 14 653.2 85.0 14 646.3 72.8 12a 632.7 129.4 13 698.1 94.2 13 654.6 112.1 13 672.9 117.4 7 668.6 128.6 7 601.7 151.5 6 5933 1053 6 Mean SD N Mean SD N Mean SD N Mean SD N 340.7 65.2 14 343.6 74.1 14 297.9 60.5 14 350.4 56.4 14 391.8 72.8 14 386.8 60.2 14 347.1 40.3 14 352.9 67.3 14 419.3 80.0 14 380.7 96.1 14 381.4 91.9 14 335.0 54.9 14 479.6 85.8 13 457.9 95.0 12 447.5 69.1 14 460.0 92.7 12 426.4 96.6 7 390.7 63.9 7 386.9 143.6 8 407.1 70.5 7 a Testing error, one data point missing. 030775 st 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 1 of 3, Page: 68 Table 13: Forelimb Grip Performance (g), Descriptive Statistics Cone. Male. 0 ppm 200 ppm 630 ppm 2000 ppm Female. 0 ppm 200 ppm 630 ppm 2000 ppm Pre-Exp. IMonth 2Month 3Month Post-Exp. Mean SD N Mean SD N Mean SD N Mean SD N 728.6 112.8 14 792.1 95.3 14 768.6 138.0 14 732.9 95.9 14 719.3 92.8 14 698.2 57.9 14 750.0 107.8 14 682.9 82.2 14 692.5 79.3 14 683.6 83.6 14 706.8 74.6 14 670.4 104.6 14 683.3 85.5 12 668.8 98.2 13 681.5 115.4 13 619.6 148.7 13 948.6 207.2 7 954.3 111.0 7 967.5 241.0 6 815.0 127.8 6 Mean SD N Mean SD N Mean SD N Mean SD N 596.4 68.0 14 618.9 73.4 14 602.5 71.2 14 564.3 81.6 14 616.8 127.6 14 645.7 61.2 14 596.4 88.0 14 585.4 94.4 14 532.1 80.2 14 542.5 53.8 14 538.9 61.8 14 500.7 85.1 14 581.9 60.1 13 541.3 88.8 12 514.3 96.1 14 467.5 96.3 12 775.0 171.2 7 646.4 154.2 7 583.1 115.8 8 553.6 121.7 7 a Testing error, one data point missing. 03O-'16 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 1 of 3, Page: 69 Table 14: Grip Performance Pre-Exposure, Statistical Analyse$a Level/Covariate Overall Treatment (T) Sex (S) Group (G) TXG Contrasts TXS 200 vs 0 630 vs 0 2000 vs 0 Covariate /Body weight MANCOVA 0.128 0.191 0.094 0.792 0.307 0.224 0.636 0.313 0.006 ANCOVA HLGPb FLGP0 0.576 0.221 0.091 0.118 0.353 0.065 0.879 0.482 0.105 0.841 0.875 0.997 0.234 0.090 0.363 0.577 0.002 0.798 Values are actual p values; highlighted values are MANCOVA o < 0.02, ANCOVA a < 0.01. HLGP Hindi imb Grip Performance FLGP Forelimb Grip Performance 1,1,1-T subchroruc neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 1 of 3, Page: 70 Table 15: Grip Performance 1 Month, Statistical Analyses3 Level/Covariate Overall Treatment (T) Sex (S) Group (G) TXG Contrasts TXS 200 vs 0 630 vs 0 2000 vs 0 Covariate /Body weight MANCOVA 0.560 0.068 0.752 0.795 0.276 0.950 0.746 0.204 0.698 ANCOVA HLGPb FLGPc 0.523 0.060 0.790 0.775 0.265 0.806 0.483 0.166 0.617 0.391 0.123 0.466 0.572 0.244 0.851 0.802 0.212 0.519 Values are actual p values HLGP Hindlimb Grip Performance FLGP Forelimb Grip Performance 030118 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 1 of 3, Page: 71 Table 16: Grip Performance 2 Month, Statistical Analysesa Level/Covariate Overall Treatment (T) Sex (S) Group (G) TXG TXS Contrasts 200 vs 0 630 vs 0 2000 vs 0 Covariate /Body weight MANCOVA 0.686 0.003 0.835 0.460 0.190 0.892 0.921 0.362 0.695 ANCOVA HLGPb FLGPC 0.911 0.001 0.557 0.930 0.312 0.151 0.858 0.154 0.042 0.901 0.632 0.965 0.563 0.566 0.975 0.689 0.178 0.555 Values are actual p values; highlighted values are MANCOVA a < 0.02, ANCOVA a < 0.01. HLGP Hindlimb Grip Performance FLGP Forelimb Grip Performance SL 030779 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 1 of 3, Page: 72 Table 17: Grip Performance 3 Month, Statistical Analysesa Level/Covariate Overall Treatment (T) Sex (S) Group (G) TXG TXS Contrasts 200 vs u 630 vs 0 2000 vs 0 Covariate /Body weight MANCOVA 0.110 0.078 0.053 0.431 0.620 0.556 0.378 0.015 0.323 ANCOVA HLGPb FLGPc 0.711 0.179 0.034 0.036 0.020 0.220 0.197 0.878 0.310 0.692 0.462 0.342 0.700 0.222 0.779 0.004 0.211 0.593 Values are actual p values; highlighted values are MANCOVA a < 0.02, ANCOVA a < 0.01. HLGP Hindlimb Grip Performance FLGP Forelimb Grip Performance 030780 SL 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 1 of 3, Page: 73 Table 18: Grip Performance Post-Exposure, Statistical Analyses3 Level/Covariate Overall Treatment (T) Sex (S) Group (G) TXG TXS Contrasts 200 vs 0 630 vs 0 2000 vs 0 Co variate /Body weight MANCOVA 0.302 0.300 0.940 0.574 0.417 0.584 0.333 0.044 0.999 ANCOVA HLGPb ELGI* 0.642 0.151 0.733 0.086 0.235 0.952 0.528 0.538 0.661 0.342 0.716 0.299 0.265 0.167 0.347 0.012 0.993 0.998 Values arc actual p values HLGP Hindlimb Grip Performance FLGP Forelimb Grip Performance SL 030781 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 1 of 3, Page: 74 Table 19: Body Temperatures (C), Descriptive Statistics Concentration Male, 0 ppm Mean SD N FEPT 37.6 0.6 12 SEPT 37.8 0.6 12 200 ppm Mean SD N 38.1 0.6 12 38.1 0.5 12 630 ppm Mean SD N 37.9 0.5 12 38.0 0.4 12 2000 ppm Mean SD N 37.8 0.5 lla 38.2 0.5 lla Female. 0 ppm Mean SD N 37.9 0.5 12 37.9 0.5 12 200 ppm Mean SD N 37.9 0.3 12 38.0 0.3 12 630 ppm Mean SD N 37.8 0.6 12 37.9 0.7 12 2000 ppm Mean SD N 37.8 0.5 11* 38.0 0.5 12 * One temperature inadvertently not recorded. a Sample size one less due to animal death. FEPT Body temperature before FEP recording. SEPT Body temperature before SEP recording. ABRCT Body temperature before ABR click recording. ABRCT 37.8 0.5 12 38.1 0.5 12 38.0 0.4 12 38.2 0.5 lla 38.1 0.6 12 38.0 0.3 12 37.9 0.6 12 38.0 0.5 12 SL 030782 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 1 of 3, Page: 75 Table 19, continued: Body Temperatures (C), Descriptive Statistics Concentration Male, 0 ppm Mean SD N ABRTT 37.8 0.5 12 CNAPT 25.0 0.2 12 200 ppm Mean SD N 38.1 0.5 12 25.1 0.4 12 630 ppm Mean SD N 38.0 0.4 12 25.2 0.4 12 2000 ppm Female. 0 ppm Mean SD N Mean SD N 38.2 0.5 lla 38.1 0.6 12 25.1 0.5 lla 25.0 0.3 12 200 ppm Mean SD N 38.0 0.3 12 24.9 0.2 12 630 ppm Mean SD N 37.9 0.6 12 25.0 0.2 12 2000 ppm Mean SD N 38.0 0.5 12 a Sample size one less due to animal death. 24.9 0.2 12 ABRTT CNAPT CFRT Body temperature before ABR tone recordings. Tail temperature before CNAP recording. Body temperature before CFR recording. CFRT 37.8 0.6 12 38.2 0.5 12 38.0 0.4 12 38.1 0.5 lla 38.1 0.6 12 37.9 0.4 12 37.9 0.7 12 38.0 0.3 12 SL 030783 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 1 of 3, Page: 76 Table 20: Body Temperatures, Statistical Analyses3 Overall Contrasts Covariate Level/Covariate Treatment (T) Sex (S) Group (G) TXG TXS 200 vs 0 630 vs 0 2000 vs 0 / Body weight FEPT 0.350 0.644 0.931 0.458 0.584 0.096 0.659 0.800 0.640 ANCOVA SEPT ABRCT 0.381 0.886 0.668 0.931 0.858 0.801 0.311 0.753 0.689 0.461 0.193 0.460 0.377 0.782 0.105 0.279 0.984 0.961 a Values are actual p values. SL* 030^ 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 1 of 3, Page: 77 Table 20, continued: Body Temperatures, Statistical Analyses3 Overall Contrasts Covariate Uvgi/Cgvariate Treatment (T) Sex (S) Group (G) TXG TXS 200 vs 0 630 vs 0 2000 vs 0 /Body weight ANCOVA ABRTT CNAPT 0.574 0.937 0.984 0.884 0.530 0.224 0.522 0.443 0.353 0.652 0.187 0.410 0.369 0.929 0.592 0.794 0.906 0.982 CEPI 0.631 0.443 0.873 0.625 0.244 0.277 0.661 0.277 0.468 a Values are actual p values. SL 030785 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 1 of 3, Page: 78 Table 20, continued: Body Temperatures, Statistical Analyses* Overall Contrasts Covariate Level/Co variate Treatment (T) Sex (S) Group (G) TXG TXS 200 vs 0 630 vs 0 2000 vs 0 / Body weight MANCOVAb 0.858 0.633 0.884 0.648 0.800 0.512 0.903 0.615 0.729 a Values are actual p values. b MANCOVA includes: FEPT, CFRT, ABRCT, ABRTT, SEPT, CNAPT SL 030786 1,1,1-T subchronic neurotoxicity Figure 3: Flash Evoked Potentials (FEP) Males The Dow Chemical Company Project ID: K-001716-091 Volume 1 of 3, Page: 79 1,1,1-T subchronic neurotoxicity Figure 4: Flash Evoked Potentials (FEP) Females Th Dow Chemical Company Project ID: K-001716-091 Volume 1 of 3, Page: 80 Latency (msec) 030788 SL 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 1 of 3, Page: 81 Table 21: FEF early latency, data window=35-95.1 msec. Descriptive Statictics Concentration Male. 0 ppm 200 ppm 630 ppm 2000 ppm Female, 0 ppm 200 ppm 630 ppm 2000 ppm Lat Diff (msec) Mean SD N 0.65 2.41 12 Mean SD N -0.55 2.28 12 Mean SD N -0.65 1.62 12 Mean SD N -0.41 3.30 11* Mean SD N 1.03 3.27 12 Mean SD N -.90 1.37 12 Mean SD N 0.96* 3.54 11 Mean SD N 0.03 2.70 12 Correl (r) 0.69 0.16 12 0.61 0.18 12 0.68 0.18 12 0.58 0.31 11* 0.68 0.30 12 0.82 0.12 12 0.67 0.18 12 0.74 0.12 12 * One data point removed to establish homogeneity of variance. a Sample size one less due to animal death. Power (RMS uV) 18.70 5.61 12 21.38 7.67 12 20.85 12.36 12 23.15 12.38 11* 23.03 13.42 12 22.65 10.40 12 16.00 5.03 12 19.67 8.20 12 SL 030789 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume l of 3, Page: 82 Table 22: FEP early latency, data window=35-95.1 msec. Statistical Analyses4 ANCOVA Overall Contrasts Covariates Level/Covariate MANCOVA Treatment (T) Sex (S) Group (G) 0.616 0.371 0.004 TXG 0.099 TXS 0.293 200 vs 0 0.353 630 vs 0 2000 vs 0 0.563 0.444 /Body weight 0.626 /Temperature 0.001 Lat Diff 0.363 0.638 0.054 0.010 0.449 0.109 0.426 0.155 0.789 0.001 Correlb 0.555 0.267 0.005 0.811 0.209 0.432 0.981 0.500 0.620 0.059 Power 0.717 0.439 0.683 0.431 0.438 0.931 0.307 0.879 0.367 0.007 a Values are actual p values; highlighted values are MANCOVA a < 0.02, ANCOVA a < 0.01. b Arcsine transformation was performed on correlation data before statistical analysis. 03019 SL 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 1 of 3, Page: 83 Table 23: FEP midlatency, data window=94.5-280 msec, Descriptive Statistics Concentration Male. 0 ppm 200 ppm 630 ppm 2000 ppm Female, 0 ppm 200 ppm 630 ppm 2000 ppm Lat Diff (msec) Mean SD N 0.50 9.81 12 Mean SD N -3.63 9.63 12 Mean SD N -0.88 6.81 12 Mean SD N 1.23 10.24 11* Mean SD N -0.88 6.15 12 Mean SD N -13.13 7.35 12 Mean SD N -3.00 12.42 12 Mean SD N -7.75 9.22 12 Correl (r) 0.96** 0.06 10 0.93 0.08 12 0.97 0.04 12 0.94 0.04 11* 0.97* 0.03 11 0.91** 0.08 10 0.90 0.09 12 0.93* 0.07 11 * One data point removed to establish homogeneity of variance. ** Two data points removed to establish homogeneity of variance. a Sample size one less due to animal death. Power (RMS llV) 43.56 21.35 12 45.79 18.04 12 51.44 23.73 12 50.98 29.06 11* 40.45 16.37 12 34.51 14.23 12 36.27 15.47 12 40.72 19.79 12 SL 030791 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 1 of 3, Page: 84 Table 24: FEP midlatency, data window=94.5-280 msec, Statistical Analysesa Overall Contrasts Covariates Level/Covariate MANCOVA Treatment (T) Sex (S) 0.085 0.822 Group (G) 0.099 TXG 0.092 TXS 0.545 200 vs 0 0.005 630 vs 0 0.469 2000 vs 0 0.310 /Body weight 0.953 /Temperature 0.005 ANCOVA Lat Diff Correlb Power 0.064 0.634 0.132 0.434 0.441 0.744 0.020 0.642 0.451 0.900 0.501 0.020 0.287 0.238 0.929 0.011 0.477 0.377 0.021 0.174 0.109 0.140 0.567 0.881 0.893 0.724 0.736 0.113 0.676 0.002 a Values are actual p values; highlighted values are MANCOVA a < 0.02, ANCOVA a < 0.01. b Arcsine transformation was performed on correlation data before statistical analysis. SL 030792 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716*091 Volume 1 of 3, Page: 85 Table 25: FEP N3 Amplitude \xV, Descriptive Statistics Concentration Male, 0 ppm 200 ppm 630 ppm 2000 ppm Female, 0 ppm \ 200 ppm 630 ppm 2000 ppm Mean SD N Mean SD N Mean SD N Mean SD N Mean SD N Mean SD N Mean SD N Mean SD N a Sample size one less due to animal death. Amplitude 82.58 45.99 12 86.75 37,27 12 98.36 45.83 12 85.96 51.42 lla 60.48 37.51 12 62.74 37.79 12 63.81 34.64 12 70.83 44.26 12 SL 030793 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 1 of 3, Page: 86 Table 26: FEP N3 Amplitude, Statistical Analyses* Level/Covariate ANCOVA Overall Treatment (T) Sex (S) Group (G) 0.836 0.258 0.991 Contrasts Covariates TXG TXS 200 vs 0 630 vs 0 2000 vs 0 /Body weight /Temperature 0.059 0.896 0.921 0.471 0.702 0.655 0.050 a Values are actual p values. SL 030 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 1 of 3, Page: 87 Table 27: CFR Signal to Noise Ratio, Descriptive Statistics Concentration Male, 0 ppm 200 ppm 630 ppm 2000 ppm Female, 0 ppm 200 ppm 630 ppm 2000 ppm Mean SD N Mean SD N Mean SD N Mean SD N Mean SD N Mean SD N Mean SD N Mean SD N a Sample size one less due to animal death. Ratio 1,52 0.70 12 1.03 0.64 12 1.30 1.28 12 1.59 0.98 na 1.59 1.46 12 2.17 2.08 12 1.61 1.51 12 1.52 1.76 12 SL 030795 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume l of 3, Page: 88 Table 28: CFR Signal to Noise Ratio, Statistical Analyses* Level/Covariate Overall Treatment (T) Sex (S) Group (G) TXG TXS Contrasts 200 vs 0 630 vs 0 2000 vs 0 Covariates /Body weight /Temperature ANCOVA 0.985 0.657 0.627 0.769 0.516 0.873 0.831 0.944 0.446 0.677 a Values are actual p values. SL 030796 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 1 of 3, Page: 89 Figure 5: Auditory Brainstem Responses to Clicks Males SL 030797 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 1 of 3, Page: 90 Figure 6: Auditory Brainstem Responses to Clicks Females 6^ 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 1 of 3, Page: 91 Table 29: ABR Click, Data Windowsl-5 msec. Descriptive Statistics Concentration Male. 0 ppm 200 ppm 630 ppm 2000 ppm Female. 0 ppm 200 ppm 630 ppm 2000 ppm Lat Diff (msec) Mean SD N 0.02 0.06 12 Mean SD N 0.01 0.06 12 Mean SD N 0.02 0.03 12 Mean SD N 0.03 0.07 lla Mean SD N 0.04 0.04 12 Mean SD N 0.02 0.03 12 Mean SD N 0.02 0.05 12 Mean SD N 0.03 0.05 12 Correl (r) 0.94 0.04 12 0.91 0.09 12 0.93 0.09 12 0.93 0.05 lla 0.93 0.03 12 0.95 0.02 12 0.93* 0.03 11 0.93 0.04 12 * One data point removed to establish homogeneity of variance. a Sample size one less due to animal death. Power (RMS llV) 2.36 0.66 12 2.27 0.75 12 2.31 0.44 12 2.24 0.70 lla 2.62 0.66 12 2.76 0.28 12 2.58 0.57 12 2.66 0.68 12 SL 030799 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 1 of 3, Page: 92 Table 30: ABR Click, Data Window=l-5 msec. Statistical Analyses3 Overall Contrasts Covariates Level/Covariate MANCOVA Treatment (T) Sex (S) Group (G) 0.988 0.705 0.026 TXG 0.306 TXS 0.893 200 vs 0 0.881 630 vs 0 2000 vs 0 0.955 0.945 / Body weight 0.737 /Temperature 0.0003 ANCOVA Lat Diff Con-elb 0.676 0.291 0.020 0.956 0.846 0.090 0.184 0.824 0.612 0.501 0.482 0.943 0.753 0.623 0.943 0.681 0.398 0.895 0.0001 0.954 Power 0.978 0.841 0.004 0.110 0.856 0.950 0.873 0.735 0.317 0.488 a Values are actual p values; highlighted values are MANCOVA a < 0.02, ANCOVA a < 0.01. b Arcsine transformation was performed on correlation data before statistical analysis. SL 030800 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 1 of 3, Page: 93 Figure 7: Auditory Brainstem Responses to 10 kHz Tone Pips Males 2000 ppm 3080i U.I-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 1 of 3, Page: 94 Figure 8: Auditory Brainstem Responses to 10 kHz Tone Pips Females SL 030802 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 1 of 3, Page: 95 Table 31: ABR tone 10 kHz, Data Window=1.5-5.9 msec. Descriptive Statistics Concentration Male. 0 ppm 200 ppm 630 ppm 2000 ppm Female, 0 ppm 200 ppm 630 ppm 2000 ppm Lat Diff (msec) Mean SD N 0.03 0.09 12 Mean SD N 0.01 0.07 12 Mean SD N 0.05 0.06 12 Mean SD N 0.01 0.10 lla Mean SD N 0.01 0.05 12 Mean SD N -0.01 0.04 12 Mean SD N 0.00 0.08 12 Mean SD N 0.01 0.10 12 Correl (r) 0.85 0.11 12 0.69* 0.24 11 0.84 0.09 12 0.78 0.20 lla 0.83 0.11 12 0.81 0.11 12 0.82 0.08 12 0.82 0.14 12 * One data point removed to establish homogeneity of variance. a Sample size one less due to animal death. Power (RMS uV) 0.63 0.17 12 0.62 0.18 12 0.63 0.12 12 0.63 0.11 lla 0.77 0.20 12 0.75 0.10 12 0.76 0.10 12 0.82 0.24 12 SL 030803 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-00171^091 Volume 1 of 3, Page: 96 Table 32: ABR tone 10 kHz, Data Window-1.5-5.9 msec. Statistical Analyses3 Overall Contrasts Covariates Level/Covariate MANCOVA Treatment (T) Sex (S) 0.392 0.620 Group (G) 0.214 TXG 0.564 TXS 0.479 200 vs 0 0.109 630 vs 0 0.947 2000 vs 0 0.521 / Body weight 0.987 /Temperature 0.0001 ANCOVA Lat Diff Correlb Power 0.700 0.671 0.177 0.997 0.917 0.237 0.245 0.051 0.110 0.355 0.251 0.818 0.455 0.335 0.840 0.545 0.599 0.769 0.041 0.753 0.328 0.898 0.968 0.605 0.975 0.821 0.0001 0.771 0.876 0.413 a Values are actual p values; highlighted values are MANCOVA a < 0.02, ANCOVA a < 0.01. b Arcsine transformation was performed on correlation data before statistical analysis. SL 030804 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 1 of 3, Page: 97 Figure 9: Auditory Brainstem Responses to 30 kHz Tone Pips Males 2000 ppm SL 030805 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 1 of 3, Page: 98 Figure 10: Auditory Brainstem Responses to 30 kHz Tone Pips Females SL 030806 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 1 of 3, Page: 99 Table 33: ABR tone 30 kHz, Data Window=l.5-5.5 msec, Descriptive Statistics Concentration Male, 0 ppm 200 ppm 630 ppm 2000 ppm Female. 0 ppm 200 ppm 630 ppm 2000 ppm Lat Diff (msec) Mean SD N 0.02 0.09 12 Mean SD N 0.00 0.11 12 Mean SD N 0.01 0.07 12 Mean SD N 0.09 0.17 lla Mean SD N 0.05 0.06 12 Mean SD N 0.09 0.10 12 Mean SD N 0.04 0.11 12 Mean SD N 0.07 0.11 12 a Sample size one less due to animal death. Correl (r) 0.65 0.24 12 0.74 0.13 12 0.76 0.13 12 0.77 0.13 lla 0.76 0.15 12 0.77 0.14 12 0.71 0.12 12 0.79 0.15 12 Power (RMS tiV) 0.47 0.14 12 0.56 0.19 12 0.55 0.12 12 0.50 0.14 lla 0.59 0.19 12 0.65 0.18 12 0.63 0.19 12 0.65 0.16 12 SL 030807 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 1 of 3, Page: 100 Table 34: ABR tone 30 kHz, Data Window=l.5-5.5 msec. Statistical Analvsesa Overall Contrasts Covariates Level/Covariate MANCOVA Treatment (T) Sex (S) 0.168 0.308 Group (G) 0.185 TXG 0.958 TX S 0.341 200 vs 0 0.189 630 vs 0 0.629 2000 vs 0 0.026 /Body weight 0.550 /Temperature 0.001 ANCOVA Lat Diff Correlb Power 0.155 0.163 0.163 0.447 0.483 0.098 0.501 0.656 0.091 0.605 0.234 0.829 0.313 0.738 0.815 0.375 0.954 0.045 0.227 0.488 0.130 0.159 0.229 0.465 0.267 0.645 0.0001 0.690 0.730 0.095 a Values are actual p values; highlighted values are MANCOVA a < 0.02, ANCOVA a < 0.01. b Arcsine transformation was performed on correlation data before statistical analysis. SL 030808 1,11*T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 1 of 3, Page: 101 Figure 11: Somatosensory Evoked Potential Recorded from the Sensory Cortex (SEF-S) 7 Males SL 030809 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID. K-001716-091 Volume 1 of 3, Page: 102 Figure 12: Somatosensory Evoked Potential Recorded from the Sensory Cortex (SEP-S) Females 030^ 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 1 of 3, Page: 103 Table 35: SEP-S early latency, data window=4-16.5 msec. Descriptive Statistics Concentration Male, 0 ppm 200 ppm 630 ppm 2000 ppm Female, 0 ppm 200 ppm 630 ppm 2000 ppm Lat Diff (msec) Mean SD N 0.13 0.72 12 Mean SD N -0.44 1.03 12 Mean SD N 0.28 0.61 12 Mean SD N -0.52 1.02 11* Mean SD N -0.04 0.51 12 Mean SD N 0.03 0.24 12 Mean SD N -0.07 0.62 12 Mean SD N 0.17* 0.43 11 Correl (r) 0.82 0.30 12 0.60 0.45 12 0.67 0.41 12 0.69 0.38 11* 0.60 0.32 12 0.71 0.33 12 0.68 0.33 12 0.66 0.31 12 * One data point removed to establish homogeneity of variance. a Sample size one less due to animal death. Power (RMS llV) 7.24 2.92 12 7.08 3.38 12 7.37 3.38 12 6.96 2.78 11* 8.12 3.05 12 8.67 3.58 12 10.92 4.80 12 8.28 3.23 12 SL 030811 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume l of 3, Page: 104 Table 36: SEP-S early latency, data window=4-16.5 msec. Statistical Analyses3 Overall Contrasts Covariates Level/Covariate MANCOVA Treatment (T) Sex (S) Group (G) 0.717 0.360 0.194 TXG 0.311 TXS 0.118 200 vs 0 630 vs 0 2000 vs 0 0.833 0.527 0.915 /Body weight /Temperature 0.324 0.0001 ANCOVA Lat Diff Correib 0.428 0.171 0.160 0.938 0.470 0.101 0.057 0.678 0.033 0.579 0.405 0.490 0.556 0.543 0.712 0.860 0.235 0.552 0.0001 0.785 Power 0.268 0.918 0.132 0.551 0.363 0.685 0.213 0.590 0.352 0.0001 a Values are actual p values; highlighted values are MANCOVA a < 0.02, ANCOVA a < 0.01. b Arcsine transformation was performed on correlation data before statistical analysis. 030812 SL 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 1 of 3, Page: 105 Table 37: SEP-S midlatency, data window=16.5-90 msec. Descriptive Statistics Concentration Male, 0 ppm 200 ppm 630 ppm 2000 ppm Female, 0 ppm 200 ppm 630 ppm 2000 ppm Lat Diff (msec) Mean SD N 0.10 2.36 12 Mean SD NT -1.77 2.09 12 Mean SD N -0.47 2.48 12 Mean SD N -1.78 2.91 lla Mean SD N 0.07 3.20 12 Mean SD N -0.43 3.20 12 Mean SD N -0.67 3.00 12 Mean SD N 0.00 2.09 12 a Sample size one less due to animal death. Correl (r) 0.97 0.02 12 0.96 0.02 12 0.96 0.04 12 0.96 0.04 IP 0.96 0.06 12 0.96 0.03 12 0.96 0.02 12 0.97 0.03 12 Power (RMS llV) 62.57 15.30 12 52.40 10.57 12 60.30 11.66 12 62.43 14.27 IP 69.50 14.68 12 67.82 13.66 12 67.08 20.47 12 70.94 17.84 12 SL 030813 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 1 of 3, Page: 106 Table 38: SEP-S midlatency, data window^ 16.5-90 msec, Statistical Analyses3 Overall Contrasts Covariates Level/Covariate MANCOVA Treatment (T) Sex (S) Group (G) 0.492 0.722 0.024 TXG 0.925 TXS 0.732 200 vs 0 0.071 630 vs 0 0.577 2000 vs 0 0.698 /Body weight 0.671 /Temperature 0.008 ANCOVA Lat Diff Correlb Power 0.593 0.788 0.404 0.418 0.603 0.187 0.466 0.324 0.582 0.310 0.727 0.920 0.686 0.277 0.394 0.998 0.236 0.868 0.006 0.779 0.500 0.052 0.387 0.668 0.986 0.288 0.234 0.710 0.468 0.001 a Values are actual p values; highlighted values are MANCOVA o < 0.02, ANCOVA a < 0.01. b Arcsine transformation was performed on correlation data before statistical analysis. SL 030814 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 1 of 3, Page: 107 Figure 13: Somatosensory Evoked Potential Recorded from the Cerebellum (SEP-C) Males SL 030815 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 1 of 3, Page: 108 Figure 14: Somatosensory Evoked Potential Recorded from the Cerebellum (SEP-C) Females 0308l6 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 1 of 3, Page: 109 Table 39: SEP-C early latency, data window=4-18 msec. Descriptive Statistics Concentration Male. 0 ppm 200 ppm 630 ppm 2000 ppm Female, 0 ppm 200 ppm 630 ppm 2000 ppm Lat Diff (msec) Mean SD N -0.01 0.40 12 Mean SD N -0.25 0.34 12 Mean SD N 0.06 0.37 12 Mean SD N 0.05 0.39 lla Mean SD N 0.10 0.31 12 Mean SD N 0.22 0.25 12 Mean SD N 0.24 0.27 12 Mean SD N 0.20 0.21 12 a Sample size one less due to animal death. Correl (r) 0.89 0.05 12 0.89 0.06 12 0.85 0.07 12 0.85 0.11 lla 0.71 0.19 12 0.79 0.07 12 0.77 0.11 12 0.79 0.17 12 Power (RMS uV) 7.31 2.11 12 8.35 3.25 12 6.86 2.09 12 8.74 3.62 lla 6.69 1.89 12 7.50 2.86 12 6.35 2.42 12 8.19 2.60 12 SL 030817 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 1 of 3, Page: 110 Table 40: SEP-C early latency, data window=4-18 msec. Statistical Analysesa Overall Contrasts Covariates Level/Covariate MANCOVA Treatment (T) Sex (S) Group (G) 0.317 0.0001 0.892 TXG 0.870 TXS 200 vs 0 0.340 0.830 630 vs 0 2000 vs 0 0.394 0.406 /Body weight /Temperature 0.001 0.0001 ANCOVA Lat Diff Correlb Power 0.211 0.0001 0.567 0.817 0.164 0.738 0.140 0.273 0.0001 0.018 0.806 0.234 0.683 0.970 0.189 0.467 0.835 0.876 0.906 0.016 0.141 0.478 0.687 0.292 0.866 0.562 0.300 0.219 0.334 0.0001 a Values are actual p values; highlighted values are MANCOVA a < 0.02, ANCOVA a < 0.01. b Arcsine transformation was performed on correlation data before statistical analysis. SL 030818 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 1 of 3, Page: 111 Table 41: SEP-C midlatency, data window=18-70 msec. Descriptive Statistics Concentration M?l$. 0 ppm 200 ppm 630 ppm 2000 ppm Female. 0 ppm 200 ppm 630 ppm 2000 ppm Lat Diff (msec) Mean SD N -0.10 1.36 12 Mean SD N -0.10 1.26 12 Mean SD N 0.37 1.56 12 Mean SD N 0.62 0.79 lla Mean SD N -0.13 1.20 12 Mean SD N -0.27 1.25 12 Mean SD N -0.20 0.93 12 Mean SD N 0.10 1.20 12 a Sample size one less due to animal death. Correl (r) 0.98 0.01 12 0.97 0.02 12 0.97 0.03 12 0.97 0.03 lla 0.95 0.04 12 0.96 0.04 12 0.97 0.03 12 0.96 0.03 12 Power (RMS nVl 27.94 4.75 12 30.20 4.84 12 31.26 5.25 12 30.53 6.85 lla 33.23 8.73 12 36.10 8.14 12 34.82 10.65 12 37.74 9.26 12 SI 030819 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 1 of 3, Page*. 112 Table 42: SEP-C midlatency, data window=18-70 msec, Statistical Analyses4 Overall Contrasts Covariates Level/Covariate MANCOVA Treatment (T) Sex (S) Group (G) 0.867 0.245 0.633 TXG 0.892 TXS 0.806 200 vs 0 0.843 630 vs 0 0.815 2000 vs 0 0.393 /Body weight 0.043 /Temperature 0.0001 ANCOVA Lat Diff Correlb Power 0.493 0.062 0.444 0.783 0.334 0.627 0.777 0.539 0.478 0.978 0.880 0.680 0.422 0.550 0.587 0.910 0.553 0.210 0.632 0.710 0.607 0567 0.406 0.337 0.025 0.742 0.118 0.504 0.664 0.0001 a Values are actual p values; highlighted values are MANCOVA a < 0.02, ANCOVA a < 0.01. b Arcsine transformation was performed on correlation data before statistical analysis. 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 1 of 3, Page: 113 Figure 15: Caudal Nerve Action Potential, Single-Stimulus Males SL 3082l 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 1 of 3, Page: 114 Figure 16: Caudal Nerve Action Potential, Single-Stimulus Females Latency (msec) SL 030^22 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-OOlTlfi-Wl Volume 1 of 3, Page: 115 Table 43: CNAP single stimulus, data window=2.8-7.4 msec, Descriptive Statistics Concentration Male, 0 ppm 200 ppm 630 ppm 2000 ppm Female. 0 dditi 200 ppm 630 ppm 2000 ppm Lat Diff (msec) Mean SD N 0.08 0.30 12 Mean SD N -0.08 0.15 12 Mean SD N 0.12 0.26 12 Mean SD N 0.07 0.30 lla Mean SD N 0.02 0.16 12 Mean SD N 0.13 0.30 12 Mean SD N 0.03 0.22 12 Mean SD N -0.02 0.16 12 a Sample size one or two less due to animal death. Correi (r) 0.94 0.04 12 0.95 0.03 12 0.95 0.04 12 0.94 0.03 lla 0.98 0.02 12 0.94 0.07 12 0.97 0.02 12 0.95 0.04 12 Power (RMS uV) 15.34 5.33 12 16.77 4.26 12 12.83 4.56 12 13.12 3.74 lla 17.72 3.96 12 16.99 4.38 12 19.99 6.48 12 17.80 8.45 12 SL 30823 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 1 of 3, Page: 116 Table 44: CNAP, single stimulus, data wmdow=2.8*7.4 msec. Statistical Analyses4 Overall Contrasts Covariates Level/Covariate MANCOVA Treatment (T) Sex (S) Group (G) 0.572 0.902 0.149 TXG 0.414 TXS 0.073 200 vs 0 630 vs 0 2000 vs 0 0.385 0.911 0.432 / Body weight /Temperature 0.793 0.092 ANCOVA Lat Diff Correlb Power 0.797 0.583 0.328 0.180 0.556 0.294 0.827 0.817 0.024 0.091 0.061 0.793 0.127 0.236 0.129 0.853 0.497 0.851 0.721 0.074 0.102 0.990 0.132 0.794 0.148 0.820 0.938 0.497 0.547 0.976 a Values are actual p values. k Arcsine transformation was performed on correlation data before statistical analysis. Sh 03082A 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 1 of 3, Page: 117 Figure 17: Caudal Nerve Action Potential, Second of Paired Stimuli Males Latency (msec) Si 382S 1,1,1*T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 1 of 3, Page: 118 Figure 18: Caudal Nerve Action Potential, Second of Paired Stimuli Females 2000 ppm 8.0 . 12.0 Latency (msec) 16.0 20.0 SL 030826 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 1 of 3, Page: 119 Table 45: CNAP paired stimulus, data window=6-10 msec. Descriptive Statistics Concentration Male. 0 ppm 200 ppm 630 ppm 2000 ppm Female. 0 ppm 200 ppm 630 ppm 2000 ppm Lat Diff (msec) Mean SD N 0.16 0.39 12 Mean SD N -0.04 0.19 12 Mean SD N 0.09 0.21 12 Mean SD N 0.04 0.19 lla Mean SD N 0.04 0.20 12 Mean SD N 0.14 0.32 12 Mean SD N 0.06 0.25 12 Mean SD N 0.02 0.20 12 Correi (r) 0.94 0.04 12 0.93 0.03 12 0.95** 0.05 10 0.91* 0.08 10a 0.96 0.04 12 0.94 0.04 12 0.95 0.06 12 0.91* 0.09 11 * One data point removed to establish homogeneity of variance. * Two data points removed to establish homogeneity of variance. a Sample size one less due to animal death. Power (RMS uVl 11.92 4.59 12 13.02 4.12 12 9.80 4.35 12 9.66 3.87 lla 12.96 4.61 12 12.73 3.99 12 15.54 6.23 12 13.06 7.13 12 SL 030827 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716091 Volume 1 of 3, Page: 120 Table 46: CNAP paired stimulus, data window=6-10 msec, Statistical Analyses3 Overall Contrasts Covariates Level/Co variate MANCOVA Treatment (T) Sex (S) 0.358 0.792 Group (G) 0.088 TXG 0.219 TXS 0.353 200 vs 0 0.559 630 vs 0 0.968 2000 vs 0 0.060 / Body weight 0.733 /Temperature 0.291 ANCOVA Lat Diff Correlb 0.802 0.320 0.086 0.593 0.136 0.259 0.031 0.137 0.339 0.868 0.475 0.667 0.330 0.910 0.348 0.028 0.349 0.080 0.843 0.672 Power 0.901 0.524 0.112 0.020 0.190 0.749 0.648 0.802 0.991 0.757 a Values are actual p values. b Arcsine transformation was performed on correlation data before statistical analysis. 03028 S^ 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 1 of 3, Page: 121 Table 47: Outliers as Identified by the F-Max Test for Homogeneity Animal ID SEX Cone ppm Variable 90A3799 M 90A3792 M 90A3857 F 90A3868 F 90A3862 F 90A3811 M 90A3885 F 90A3887 F 90A3828 M 90A3824 M 90A3901 F 90A3895 F 90A3845 M 90A3893 F 0 FEP-V, 94.5-280.0 ms, correlation FEP-V, 94.5-280.0 ms, correlation 200 FEP-V, 94.5-280.0 ms, correlation ABR 10kHz, 1.5-5.9 ms, correlation 630 FEP-V, 35-95.1 ms, latency difference ABR click. 1-5 ms, correlation CNAP paired, 6-10 ms, correlation 2000 FEP-V, 94.5-280.0 ms, correlation SEP-S, 4-16.5 ms, latency difference CNAP paired, 6-10 ms, correlation CNAP paired. 6-10 ms, correlation "Mean of group after outlier was removed. Value Mean* removed 0.96 0.97 0.91 0.69 -0.90 0.93 0.95 0.93 0.17 0.91 0.91 0.604 0.656 0.000 0364 0.431 0.248 7.50 0.617 0.202 0.643 0.366 -2.80 0.172 0.589 SL 030829 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume I of 3, Page: 122 Table 48: Spontaneous or moribund animals submitted for necropsy. Animal ID Sex Dose (ppm) Reason for removal 90A3794 90A3801 M M 0 Anesthetic related death; day 64 0 Lost implant; day 93 90A3810 90A3816 M M 200 200 Spont. death (no diagnosis; day 126) Lost implant; day 94 90A3822 90A3827 90A3831 M M M 630 630 630 Moribund (loss of upper incisors; day 126) Anesthetic related death; day 63 Lost implant; day 128 90A3836 90A3846 xVr M 2000 2000 Lost implant; day 93 Congested viscera, no diagnosis; day 59 90A3858 90A3859 F F 0 Lost implant; day 94 0 Lost implant; day 121 90A3866 F 200 Anesthetic related death; day 66 90A3871 F 200 Lost implant; day 93 90A3880 F 630 Lost implant; day 113 90A3894 90A3896 F F 2000 2000 Anesthetic related death; day 66 Accident (trauma); day 67 -n o SL 030831 1,1,1-T subchronic neurotoxicity TABLE 49: GROSS PATHOLOGIC OBSERVATION50 - MAIN STUDY The Dow Cemicai Companay Study ID: K-001716-091 Volume 1 of 3, Page: 123 GROSS PATHOLOGIC OBSERVATIONS^ - MAIN STUDY SES---------------------------------------------- EXPOSURE CONCENTRATION IN PPM NUMBER OF RATS EXAMINED ---------MALES. 200 630 2000 5_ S 5 5 EXTERNAL AND SKIN WITHIN NORMAL LIMITS. ABSCESS, HEAD, FOCAL: ALI. OTHER TISSUES (COMPLETE NECROPSY PERFORMED* WITHIN NORMAL LIMITS. 554 5 00 I 0 555 5 0 DATA ARE THE NUMBER OF ANIMALS WITH THE SPECIFIED OBSERVATION. FEMALES 50 05 41 5 5 55 1,1,1-T subchronic neurotoxicity TABLE 50: GROSS PATHOLOGIC OBSERVATIONS - RECOVERY PORTION The Dow Cemical Companay Study ID: K4W1716-091 Volume 1 of 3, Page: 124 SL 030832 5i EXPOSURE CONCENTRATION IN PPM NUMBER OF RATS EXAMINED 0 700 6.10 7000 7777 777 EXTERNAL AND SKIN WITHIN NORMAL LIMITS. ABSCESS, HEAD, FOCAL: EYES HITHIN NORMAL LIMITS. OPACITY, LENS, LEFT: PREPUTIAL OR CLITORAL GLANDS HITHIN NORMAL LIMITS ABSCESS, UNILATERAL 667 6 1 101 7777 000 0 777 7 0000 777 000 67 7 100 7 e7 0l0 URINARY BLADDER HITHIN NORMAL LIMITS CALCULUSII), LUMEN: THICKENED, HALL: 7777 0 0 00 0 0 00 777 000 000 ALL OTHER TISSUES (COMPLETE NECROPSY PERFORMED} HITHIN NORMAL LIMITS. 7777 77 7 S DATA ARE THE HUMBER OF ANIMALS WITH THE SPECIFIED OBSERVATION. FIVE MALE AND FIVE FEMALE RATS FRAOM EACH GROUP HERE RANDOMLY SELECTED BY THE NEUROTOXICOLOGY GROUP FOR POSSIBLE NEUROPATHOLOGIC EVALUATION. 1 f 0 7 0 / 0 6 1 1 ? 1,1,1-T subchronic neurotoxicity Table 51: Brain Weightsa The Dow Chemical Company Project ID: K-001716-091 Volume I of 3, Page: 125 Concentration Male,, 0 ppm 200 ppm 630 ppm 2000 ppm Female. 0 ppm 200 ppm 630 ppm 2000 ppm Mean SD N Mean SD N Mean SD N Mean SD N Mean SD N Mean SD N Mean SD N Mean SD N. Brain Weight (g) 1.87 0.07 5 1.82 0.04 5 1.81 0.07 5 1.88 0.05 5 1.69 0.05 5 1.70 0.04 5 1.68 0.03 5 1.71 0.04 5 a No significant differences (ANOVA a<0.02 ). Body weight was used as a covariate and sex was used as a factor. 030833 1,1,1-T subchronic neurotoxicity TABLE 52; HISTOPATHOLOGIC OBSERVATIONS0 - MAIN STUDY ao EXPOSURE CONCENTRATION IN PPH HUMBER OF RATS EXAMINED BRAIN - CEREBELLUM (NO. OF TISSUES EXAMINED) HITHIN NORMAL LIMITS: BRAIN - CEREBRUM (NO. OF TISSUES EXAMINED) HITHIN NORMAL LIMITS; BRAIN - FQREBRAIM (MO. OF TISSUES EXAMINED) HITHIN NORMAL LIMITS: ATROPHY, GRAY MATTER, UNILATERAL, FOCAL: INFLAMMATION - CHRONIC,. MENINGES, UNILATERAL, FOCAL: INFLAMMATION - CHRONIC, MENINGES, BILATERAL, MULTIFOCAL: BRAIN - MEDULLA OBLONGATA (NO. OF TISSUES EXAMINED) HITHIN NORMAL LIMITS: BRAIN - MIDBRAIN INO. OF TISSUES EXAMINED) HITHIN NORMAL LIMITS: INFLAMMATION - CHRONIC, MENINGES, UNILATERAL, FOCAL: INFLAMMATION - CHRONIC, MENINGES, BILATERAL, MULTIFOCAL: BRAIN - PONS (NO. OF TISSUES EXAMINED) HITHIN NORMAL LIMITS: nORSAL ROOT GANGLIA (NO. OF TISSUES EXAMINED) HITHIN NORMAL LIMITS: 0 DATA ARE THE NUMBER OF ANIMALS WITH THE SPECIFIED OBSE c/l HALES-- 0 ___ 200Q a._____ a_ 55 55 55 55 55 45 00 10 00 55 55 55 23 32 00 55 55 55 55 The Dow Cemical Companay Study ID: K-001716-091 Volume 1 of 3, Page: 126 femai.es _2MQ S____ L_ 55 55 55 55 55 35 10 00 10 55 55 55 25 20 10 55 55 55 55 800 * 1,1,1-T subchronic neurotoxicity TABLE 52, continued: HISTOPATHOLOGIC OBSERVATIONS!) - MAIN STUDY The Dow Cemical Companay Study ID: K-001716-091 Volume 1 of 3, Page: 127 in in SL 030835 su EXPOSURE CONCENTRATION IN PPM HUMBER OF RATS EXAMINED EXES (NO. OE TISSUES EXAMINED) WITHIN NORMAL LIMITS: GASSERIAN GANGLIA (NO. OE TISSUES EXAMINED) WITHIN NORMAL LIMITS: PERIPHERAL NERVE - OPTIC (NO. OF TISSUES EXAMINED) WITHIN NORMAL LIMITS: PERIPHERAL NERVE - SCIATIC (NO. OF TISSUES EXAMINED) WITHIN NORMAL LIMITS: PERIPHERAL NERVE - SURAL (NO. OF TISSUES EXAMINED) WITHIN NORMAL LIMITS: PERIPHERAL NERVE - TIBIAL (NO. OF TISSUES EXAMINED) WITHIN NORMAL LIMITS: SKELETAL MUSCLE (NO. OF TISSUES EXAMINED) WITHIN NORMAL LIMITS; SPINAL CORD - CERVICAL (NO. OF TISSUES EXAMINED) WITHIN NORMAL LIMITS: SPINAL CORD - LUMBAR (NO. OF TISSUES EXAMINED) WITHIN NORMAL LIMITS: 8 DATA ARE THE NUMBER OF ANIMALS WITH THE SPECIFIED OBSERVATION. HALES___ 11___ 2M0 5____ 5__ 55 55 55 55 55 55 55 55 55 55 55 55 55 55 55 55 55 55 o 2000 5_ 55 55 55 55 55 55 55 55 55 55 55 55 5 5 55 55 1,1,1-T subchronic neurotoxicity TABLE 52, continued: HISTOPATHOLOGIC OBSERVATIONS0 - MAIN STUDY The Dow Cemical Companay Study ID: K-001716-091 Volume 1 of 3, Page: 128 SEX EXPOSURE CONCENTRATION IN PPM NUMBER OF RATS EXAMINED SPINAL NERVE ROOTS (NO. OF TISSUES EXAMINED) WITHIN NORMAL LIMITS: 0 DATA ARE THE NUMBER OF ANIMALS WITH THE SPECIFIED OBSERVATION. hales f5i______20s11_11 55 55 0 2000 5___ in in r* O' SL 030837 1,1,1 -T subchronic neurotoxicity TABLE 53; HISTOPATHOLOGIC OBSERVATIONS!! RECOVERT PORTION The Dow Cemical Companay Study ID: K-001716-091 Volume 1 of 3, Page: 129 SEX___ EXPOSURE CONCENTRATION IN PPM HUMBER OF RATS EXAMINED DIGITS (NO. OF TISSUES EXAMINED) WITHIN NORMAL LIMITS: PERIPHERAL NERVE - MEDIAN (NO. OF TISSUES EXAMINED) WITHIN NORMAL LIMITS: SKELETAL MUSCLE (NO. OF TISSUES EXAMINED) WITHIN NORMAL LIMITS: ' 0 DATA ARE THE NUMBER OF ANIMALS WITH THE SPECIFIED OBSERVATION. y i c/i i/ t_n cn v* MALES 0 2000 5___ 5_ 5 5 5 5 5 5 FEMALES 0 2000 __ 55 55 55 55 55 55 f 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 2 of 3, Page: 1 Appendix A, Water Analyses MIDLAND WATER PURIFICATION PLANT DOMESTIC WATER LABORATORY ANALYSES? SAMPLE TEMP., C TURBIDITY, NTU PH CONDUCTANCE, MICROMHOS/CM HARDNESS TOTAL (MG/L) NON-CARBONATE (MG/L) ALKALINITY TOTAL (MG/L) CARBONATE (MG/L) BICARBONATE (MG/L) CALCIUM (MG/L) MAGNESIUM (MG/L) CHLORIDES (MG/L) FLUORIDES (MG/L) CHLORINE FREE (MG/L) TOTAL (MG/L) PHOSPHATE ORTHO (MG/L) TOTAL (MG/L) IRON (MG/L) LANCELIER STABILITY INDEX JUNE 1990 14 0.09 9.2 173 75 31 44 9 35 18.0 7.4 18 1.08 0.98 -- 0.02 0.37 0.06 +0.4 JULY 1990 17 0.09 9.0 172 74 31 43 7 36 17.9 7.3 18 1.09 0.94 1.24 0,06 0.51 0.05 +0.4 AUGUST 1990 19 0.13 8.8 171 74 32 42 4 37 17.7 7.3 19 1.15 0.98 1.20 0.07 0.40 0.06 +0.2 SEPTEMBER 1990 19 0.18 8.9 170 74 32 42 4 38 17.6 7.2 18 1.10 0.99 1.25 0.08 0.35 0.07 +0.2 3 STANDARD METHODS FOR EXAMINATION OF WATER AND WASTE WATER, 16TH ED.(1985), AMERICAN PUBLIC HEALTH ASSOCIATION AND AMERICAN WATER WORKS; WASHINGTON, O.C. NTU - NEPHELOMETRIC TURBIDITY UNITS SL 030838 1,1,1 -T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 2 of 3, Page: 2 Appendix B: Chamber Analyses Temperature and Relative Humidity - Control DATE 29-May-90 30-May-90 31-May-90 l-Jun-90 4-Jun-90 5-Jun-90 6-Jun-90 7-jun-90 B-Jun-90 ll-Jun-90 12-Jun-90 13-Jun-90 14-Jun-90 15-Jun-90 18-Jun-9Q 19-Jun-90 20-Jun-90 21-Jun-90 22-Jun-90 25-Jun-90 26-Jun-90 27-Jun-90 28-Jun-90 29--Jun-90 2-JU1-90 3-JU1-90 S-Jul-90 6-Jul-90 9-JU1-90 R.H. 44 43 50 55 50 53 57 54 63 56 63 65 65 60 63 54 59 63 66 59 68 67 66 65 63 65 66 52 65 MIN TEMP. 20 20 20 21 20 20 19 20 21 21 22 21 22 21 21 21 21 21 22 22 22 22 22 22 22 21 21 21 21 MAX, TEMP 22 22 22 22 22 22 22 21 22 21 22 22 22 22 22 22 22 22 22 22 22 22 22 22 22 22 22 21 21 SL 030839 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 2 of 3, Page: 3 Appendix B, continued: Chamber Analyses Temperature and Relative Humidity - Control DATE 10-Jul-90 ll-Jul-90 12-Jul-90 13-Jul-90 16-Jul-90 n-jui-90 ia-Jui-90 19~Jul-90 20-Jul-90 23-Jul-90 24-Jul-90 25-Jul-90 26~Jul-90 27-Jul-90 30-Jul-90 31-Jul-90 l-Aug-90 2-Aug-90 3-Aug-90 6-Aug-90 7-Aug-90 8-Aug-90 9-Aug-90 10-Aug-90 13-Aug-90 14-Aug-90 15-Aug-90 16-Aug-90 I7-Aug-90 20-Aug-90 21-Aug-90 22-Aug-90 23-Aug-90 24-Aug-90 27-Aug-90 28-Aug-90 29-Aug-90 30-Aug-90 31-Aug-90 NMEAN S.D. MIN. MAX. R.H. 86 63 61 53 58 6B 66 68 72 67 68 63 65 65 68 58 62 64 65 66 61 64 63 63 65 60 68 68 67 67 68 68 69 67 70 72 65 65 66 68 62.7 6.1 43 72 MIN TEMP. 21 22 22 21 22 21 22 22 20 22 22 21 21 20 21 21 21 21 21 21 21 21 20 21 21 21 22 21 21 21 21 21 21 21 22 21 21 21 21 68 21.1 0.7 19 22 MAX. TEMP. 21 22 22 22 22 21 22 22 22 22 22 22 22 22 22 22 22 21 22 22 22 22 22 22 22 22 22 22 22 22 22 22 22 22 22 22 22 22 22 68 21.9 0.3 21 22 03** 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 2 of 3, Page: 4 Appendix B, continued: Chamber Analyses Temperature and Relative Humidity - 200 ppm ATE R.H. MIN ______________________________ TEMP. 29-May-90 50 20 30-May-90 31-May-90 l-Jun-90 4-Jun-90 5-Jun-90 6-Jun-90 7-Jun-90 8-Jun-90 ll-Jun-90 12-Jun-90 13-Jun-90 50 50 48 50 50 50 50 51 50 52 53 21 21 21 21 19 18 21 22 21 21 21 14-Jun-90 15-Jun-90 18-Jun-90 55 50 52 22 21 21 19-Jun-90 20-Jun-90 50 53 21 22 21-Jun-90 53 21 22-Jun-90 25-Jun-90 53 51 22 21 2S-Jun-90 52 22 27-Jun-90 54 22 28-Jun-90 52 21 29-Jun-90 55 21 2-Jul-90- 52 21 3-Jul-90 S3 21 5-Jul-90 53 22 fi-Jul-90 9-Jul-90 51 54 21 21 MAX. TEMP. 22 23 22 22 22 21 21 22 22 22 22 22 22 22 22 22 22 22 22 22 22 22 22 22 22 22 23 22 22 S, 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 2 of 3, Page: 5 Appendix B, continued: Chamber Analyses Temperature and Relative Humidity - 200 ppm DATE___________ R.H. lQ-Jul-90 34 U-Jul-90 50 12-Jul-90 50 13-Jul-90 50 IS-Jul-90 53 17-Jul-90 57 18-Jul-90 55 19-Jul-90 56 20-Jul-90 56 23-Jul-90 53 24-Jul-90 52 2S-Jul-90 52 26-JU1-90 52 27-Jul-90 53 30-Jul-90 57 3I-JuI-9Q 50 l-Aug-90 51 2-Aug-90 51 3-Aug-90 52 6-Aug-90 53 7-Aug-90 53 8-Aug-90 54 9-Aug-90 52 10-Aug-90 52 13-Aug-90 ' 53 I4-Aug-90 50 15-Aug-90 55 16-Aug-90 55 17-Aug-90 55 20-Aug-90 56 21-Aug-90 56 22-Aug-90 56 23-Aug-90 57 24-Aug-90 56 27-Aug-90 58 28-Aug-90 58 29-Aug-90 55 30-Aug-90 54 31-Aug-90 54 NMEAN S.D. MIN. MAX. 68 52.8 2.4 48 S3 min TEMP. 22 21 21 21 22 22 21 22 21 21 21 21 20 21 21 21 22 21 23 21 21 21 21 21 21 22 22 21 21 22 21 21 22 22 21 21 21 21 21 68 21.2 0.7 18 23 max . TEMP. 22 22 22 22 23 22 22 22 22 22 22 22 21 21 22 22 22 22 24 22 22 22 22 22 23 22 22 22 22 22 22 22 23 23 22 22 22 22 22 68 22.1 0.5 21 24 SL 030842 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 2 of 3, Page: 6 Appendix B, continued: Chamber Analyses Temperature and Relative Humidity - 630 ppm DATE R.H. MIN ______________________________ TEMP 29-May-90 50 20 30-May-90 52 20 31-May-90 51 20 l-Jun-90 49 20 4-Jun-90 52 21 S-Jun-90 51 20 6-Jun-90 50 19 7-Jun-90 51 20 8-Jun-90 53 21 ll-Jun-90 51 20 12-Jun-9Q 53 20 13-Jun-90 54 20 14-Jun-90 55 21 15-Jun-90 51 20 18-Jun-90 53 21 19-Jun-9Q 51 21 20-Jun-90 54 21 21-Jun-90 54 21 22-Jun-9Q 54 21 25-Jun-90 52 21 26-Jun-90 53 21 27-jun-90 51 21 28-Jun-90 54 21 29-Jun-90 55 21 2-Jul-90 54 21 3-JU1-90 51 20 5-Jul-90 54 21 S-Jul-90 51 21 9-Jul-90 54 20 MAX. TEMP. 22 22 22 21 22 21 21 21 22 22 21 21 22 21 21 22 21 21 22 22 21 22 22 22 21 21 22 21 22 SL 030843 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 2 of 3, Page: 7 Appendix B, continued: Chamber Analyses Temperature and Relative Humidity - 630 ppm date r.h. min ______________________________ TEMP. lO-Jul-90 54 21 ll-Jul-90 51 21 12-Jul-90 50 21 13-JU1-90 50 21 16-Jul-90 54 21 I7-Jul-90 55 21 18--Jul-90 54 21 19-Jui-90 56 21 20-JUI-9Q 56 20 23-JU1-90 54 21 24-Jul-90 53 20 25-Jul-90 53 20 26-Jul-90 54 20 27-Jul-90 54 20 30-Jul-90 55 21 31-Jul-90 50 20 l-Aug-90 51 21 2-Aug-90 51 21 3-Aug-90 53 20 S-Aug-90 54 21 7-Aug-90 50 21 a-Aug-90 51 21 9-Aug-90 51 20 10-Aug-90 52 21 13-Aug-90- 53 20 14-Aug-9Q 50 21 15-Aug-90 56 21 16-Aug-90 55 21 17-Aug-90 55 20 20-Aug-90 56 21 21-Aug-90 55 21 22-Aug-90 55 21 23-Aug-90 56 21 24-Aug-90 55 21 27-Aug-90 58 21 28-Aug-90 61 21 29-Aug-90 54 20 30-Aug-90 54 20 31-Aug-90 54 20 NMEAN S.D. MIN. MAX. 68 53.1 2.2 49 61 68 20.6 0.5 19 21 max. TEMP. 22 22 22 22 23 22 22 22 22 22 21 21 21 21 22 22 22 21 21 22 22 22 22 22 22 22 22 22 21 22 22 22 22 22 22 22 21 21 21 68 21.7 0.5 21 23 SL 030844 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 2 of 3, Page: 8 Appendix B, continued: Chamber Analyses Temperature and Relative Humidity - 2000 ppm DATE R.H. MIN ______________ '_______________ TEMP. 29-May-90 30-May-90 3I-May-90 49 51 50 20 21 20 l-Jun-90 48 20 4-Jun~90 51 19 5-Jun-90 6-Jun-90 7-Jun-90 8-Jun-90 ll-Jun-90 12-Jun-90 50 48 50 52 50 52 21 19 21 21 21 21 13-Jun-90 53 21 14-Jun-90 I5-Jun-90 ia-Jun-90 53 50 50 21 20 21 19-Jun-90 50 21 2Q-Jun-9Q 21-Jun-90 22-Jun-90 2S-Jun-90 53 50 52 50 21 21 22 21 26-Jun-90 51 22 27-Jun-90 28-Jun-90 29-Jun-90 54 53 52 21 21 21 2-Jul-90 3-Jul-90 50 52 21 20 S-Jul-90 51 21 S-Jul-90 9-Jul-90 50 52 21 21 MAX. TEMP. 23 22 22 21 21 22 21 22 22 22 22 22 22 21 22 22 22 21 22 22 22 22 22 22 22 22 22 22 22 SL 030845 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 2 of 3, Page: 9 Appendix B, continued: Chamber Analyses Temperature and Relative Humidity - 2000 ppm AXE ________ 10-Jul-90 U-Jul-90 12-Jul-90 13-JU1-90 16-Jul-90 17-Jul-90 18-Jul-90 19-JU1-90 20-Jul-90 23-JU1-9Q 24-Jul-90 25-Jul-90 26-Jul-90 27-Jul-90 30-Jul-90 31-JU1-90 l-Aug-90 2-Aug-90 3-Aug-9Q 6-Aug-90 7-Aug-90 8-Aug-90 9-Aug-9Q 10-Aug-90 13-Aug-9Q 14-Aug-90 15-Aug-90 16-Aug-90 17-Aug-90 20-Aug-90 21-Aug-90 22-Aug-90 23-Aug-90 24-Aug-90 27-Aug-90 28-Aug-90 29-Aug-90 30-Aug-90 31-Aug-90 R.H. _______________ 31 50 49 50 51 53 52 54 54 52 51 50 51 51 53 59 50 SO 51 53 50 51 50 50 51 46 54 53 52 53 53 53 53 53 55 57 51 50 51 MIN TEMP. 21 21 21 21 20 21 21 21 21 21 21 21 21 20 21 21 21 21 21 21 21 21 21 21 21 21 21 22 21 21 21 21 21 21 21 21 21 21 20 N MEAN S.Q. MIN. MAX. 68 51.4 2.0 46 59 68 20.9 0.5 19 22 MAX. TEMP. 22 22 22 22 25 22 21 22 22 22 21 22 22 22 21 22 22 21 21 22 21 22 22 22 22 22 22 22 22 22 22 22 22 22 22 22 21 21 21 68 21.9 0.6 21 25 SL 030846 1,1,1 -T subchronic neurotoxicity The Dow Chemical Company Study ID: K-001716-091 Volume 2 of 3, Page: 10 Appendix B, continued: Chamber Analyses ANALYTICAL AND NOMINAL CONCENTRATION - CONTROL DATE 29-May-90 30-May-90 31-May-90 l-Jun-90 4-Jun-90 5-Jun-90 6-Jun-9Q 7-Jun-90 B-Jun-90 1 l-Jun-90 12-Jun-91 13-Jun-90 14-Jun-90 15-Jun-90 INDIVIDUAL EXPOSURE_______________________________________________ DATA BEGAN TIME CONC. TIME CONC. TIME CONC. TIME 8:43 9:21 0.0 11:22 0.0 10:22 0.0 10:52 12:23 0.0 12:53 0.0 13:23 0.0 13:53 7:44 9:22 0.0 9:52 0.0 10:22 0.0 10:53 12:23 0.0 12:54 0.0 13:24 0.0 13:54 9:05 9:43 0.0 10:13 0.0 10:44 0.0 11:14 12:45 0.0 13:15 0.0 13:45 0.0 14:15 8:43 9:21 0.0 9:51 0.0 10:21 0.0 10:51 12:22 0.0 12:52 0.0 13:23 0.0 13:53 9:00 9:40 12:42 0.0 0.0. 10:10 13:12 0.0 0.0 10:41 13:42 0.0 0.0 11:11 14:13 8:57 9:35 0.0 10:05 0.0 10:35 0.0 11:05 12:36 0.0 13:06 0.0 13:37 0.0 14:07 9:00 9:36 0.0 10:07 0.0 10:37 0.0 11:07 12:38 0.0 13:08 0.0 13:39 0.0 14:09 8:47 9:27 0.0 9:57 0.0 10:27 0.0 10:58 12:29 0.0 12:59 0.0 13:29 0.0 13:59 8:43 9:21 0.0 9:51 0.0 10:22 0.0 10:52 12:23 0.0 12:53 0.0 13:23 0.0 13:53 8:45 9:24 0.0 9:55 0.0 10:26 0.0 10:57 12:30 0.0 13:01 0.0 13:32 0.0 14:03 8:36 9:14 0.0 9:44 0.0 10:14 0.0 10:44 12:15 0.0 12:46 0.0 13:16 0.0 13:46 8:46 9:24 0.0 9:54 0.0 10:24 0.0 10:55 12:25 0.0 12:56 0.0 13:26 0.0 13:56 8:46 9:24 0.0 9:54 0.0 10:24 0.0 10:55 12:25 0.0 12:56 0.0 13:26 0.0 13:56 8:48 9:25 0.0 9:55 0.0 10:26 0.0 10:56 12:27 0.0 12:57 0.0 13:27 0.0 13:57 CONC. 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 TIME 11:22 14:24 11:23 14:24 11:44 14 : 46 11:22 14:23 11:41 14:43 11:36 14:37 11:37 14 : 39 11:28 14:30 11:22 14:24 11:28 14:34 11:15 14:16 11:25 14:26 11:25 14:26 11:26 14:28 CONC. 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 TIME CONC. MEAN S.D. 11:52 0.0 0.0 0.0 11:53 12:15 0.0 0.0 0.0 0.0 0.0 0.0 11:52 0.0 0.0 0.0 12:11 0.0 0.0 0.0 12:06 12:08 11:58 11:52 11:59 11:45 11:55 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 11:55 0.0 0.0 0.0 11:56 0.0 0.0 0.0 N 11 11 11 11 11 11 11 11 11 11 11 11 11 11 SL 030847 SL 030848 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Study ID: K-001716-091 Volume 2 of 3, Page: 11 Appendix B, continued: Chamber Analyses ANALYTICAL AND NOMINAL CONCENTRATION - CONTROL (CONT.) DATE 18-Jun-90 19-Juii-90 20-Jun-90 21-Jun-90 22-Jun-90 25-Jun-90 26-Jun-90 2?-Jun-90 28-Jun-90 29-Jun-90 2 -Jul- 90 3-Jul-90 5-JuI-90 6-Jul-90 EXPOSURE ________________________ BEGAN TIME CONC, TIME CONC. 8:43 9:21 0.0 9 51 0.0 8:37 12:22 9:15 12:16 0.0 0.0 0.0 12 52 9 45 12 47 0.0 0.0 0.0 8:36 8:37 8:38 9:13 12:15 9:15 12:16 9:16 0.0 0.0 0.0 0.0 0.0 9 44 12 45 9 45 12 47 9 46 0.0 0.0 0.0 0.0 0.0 12:18 0.Q 12 48 0.0 8:48 8:23 9:26 12:27 9:00 0.0 0.0 0.0 9 56 12 58 9 31 0.0 0.0 0.0 8:30 12:02 9:13 0.0 0.0 12 32 9 43 0.0 0.0 8:38 12:14 9:16 12:18 0.0 0.0 0.0 12 44 9 46 12 48 0.0 0.0 0.0 8:30 8:32 9:07 12:09 9:09 0.0 0.0 0.0 9 37 12 39 9 40 0.0 0.0 0.0 8:48 8:40 8:34 12:11 9:26 12:28 9:17 12:18 9:11 12:13 0.0 0.0 0.0 0.0 0.0 0.0 0.0 12 41 9 56 12 58 9 47 12 49 9 42 12 43 0.0 0.0 0.0 0.0 0.0 0.0 0.0 TIME 10:21 13:23 10:15 13:17 10:14 13:16 10:16 13:17 10:16 13:18 10:26 13:28 10:01 13:02 10:13 13:15 10:17 13:18 10:08 13:09 10:10 13:12 10:27 13:28 10:17 13:19 10:12 13:14 INDIVIDUAL DATA CONC. TIME 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 10 51 13 53 10 46 13 47 io 44 13 46 10 46 13 47 10 47 13 49 10 56 13 58 10 31 13 33 10 43 13 45 10 47 13 48 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 10 38 13 40 10 40 13 42 10 57 13 59 10 48 13 49 10 42 13 44 CONC. 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 TIME 11:22 14:23 11:16 14:17 11:15 14:16 11:16 14:18 11.-17 14:19 11:27 14:28 11:01 14:03 11:14 14:15 11:17 14:19 11:08 14:10 11:11 14:12 11:27 14:29 11:18 14:19 11:12 14:14 CONC. 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 TIME CONC. MEAN S.D. 11:52 0.0 0.0 0.0 11:46 0.0 0.0 0.0 11:45 0.0 0.0 0.0 11:46 0.0 0.0 0.0 11:47 0.0 0,0 0.0 11:57 0.0 0.0 0.0 11:32 0.0 0.0 0.0 11:44 0.0 0.0 0.0 11:47 0.0 0.0 0.0 11:39 0.0 0,0 0.0 11:41 0.0 0.0 0.0 11:57 0.0 0.0 0.0 11:48 0.0 0.0 0.0 11:43 0.0 0.0 0.0 N 11 11 11 11 11 11 11 11 11 11 11 11 11 11 I 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Study ID: K-001716-091 Volume 2 of 3, Page: 12 Appendix 13, continued: Chamber Analyses ANALYTICAL AND NOHINAL CONCENTRATION - CONTROL (CONT.) DATE 9-Jul-90 10-Jul-90 n-jui-90 12-Jul-90 lI-Jul-90 16-Jul-90 H-Jul-90 ie-jui-90 19-Jul-90 20-Jul-90 23-Jul-90 24-Jul-90 25-Jul-90 26-JU1-90 EXPOSURE BEGAN TIME 8:33 9:11 12:12 8:52 9:28 12:29 8:30 9:07 12:09 8:45 9:22 8:37 12:23 9:13 12:14 8:52 9:30 8:58 12:31 9:36 8:20 12:37 8:58 11:59 8:45 9:22 12:23 8:42 9:19 12:20 B: 36 9:13 12:14 8:48 9:26 12:27 8:16 8:44 8:54 11:5S 9:22 12:22 CONC. 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0. 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 _____ TIME 9 41 12 43 9 58 12 59 9 38 12 39 9 52 12 53 9 44 12 44 10 00 13 01 10 06 13 07 9 28 12 29 9 52 12 53 9 49 12 50 9 43 12 44 9 56 12 57 9 24 12 25 9 52 12 53 CONC. 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 TIME 10:11 13:13 10:28 13:29 10:08 13:10 10:22 13:23 10:14 13:14 10:30 13:31 10:36 13:37 9:58 12:59 10:23 13:23 10:19 13:20 10:14 13:14 10:26 13:27 9:54 12:55 10:22 13:23 INDIVIDUAL DATA CONC. TIME 0.0 10:42 0.0 0.0 13:43 10:58 0.0 14:00 0.0 0.0 10:38 13:40 0.0 10:52 0.0 0.0 13:53 10:44 0.0 13:45 0.0 0.0 0.0 0.0 0.0 11:00 14:01 11:06 14:07 10:28 0.0 13:29 0.0 0.0 10:53 13:53 0.0 0.0 0.0 10:49 13:50 10:44 0.0 13:45 0.0 0.0 10:56 13:57 0.0 0.0 0.0 10:24 13:25 10:52 0.0 13:53 CONC. 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 TIME 11 12 14 14 11 28 14 30 11 08 14 10 11 23 14 23 11 14 14 15 11 30 14 31 11 36 14 37 10 58 13 59 11 23 14 24 11 20 14 20 11 14 14 15 11 26 14 27 10 54 13 55 11 22 14 23 CONC. 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 TIME CONC. MEAN S.D. 11:42 0.0 0.0 0.0 11:59 0.0 0.0 0.0 11:39 0.0 0.0 0.0 11:53 0.0 0.0 0.0 11:44 0.0 0.0 0.0 12:00 0.0 0.0 0.0 12:06 0.0 0.0 0.0 11:29 0.0 0.0 0.0 11:53 0.0 0.0 0.0 11:50 0.0 0.0 0.0 11:44 0.0 0.0 0.0 11:57 0.0 0.0 0.0 11:24 0.0 0.0 0.0 11:52 0.0 0.0 0.0 N 11 11 11 11 11 11 11 11 11 11 11 11 11 11 SL 030849 I SL 030850 1,1,1 -T subchronic neurotoxicity The Dow Chemical Company Study ID: K-001716-091 Volume 2 of 3, Page: 13 Appendix B, continued: Chamber Analyses ANALYTICAL AND NOMINAL CONCENTRATION - CONTROL (CONT.) DATE 27-Ju1-90 30-JU1-90 ai-jui-90 1-Aug-90 2-Aug-90 3-Aug-90 6-Aug-90 I-Aug-90 8-Aug-90 9-Aug-90 I0-Aug-90 13-Aug-90 14-Aug-90 15-Aug-90 EXPOSURE __________________________ BEGAN TIME CONC. TIME CONC. 8:41 9;19 0.0 9:49 0.0 8:50 12:20 9:27 0.0 0.0 12:50 9:57 0.0 0.0 12:28 0.0 12:58 0.0 8:25 9:03 0.0 9:33 0.0 12:04 0.0 12:34 0.0 8:25 9:03 0.0 9:33 0.0 8:29 12:04 9:07 0.0 0.0 12:34 9:37 0.0 0.0 12:07 0.0, 12:38 0.0 8:26 9:03 0.0 9:33 0.0 12:04 0.0 12:34 0.0 7:28 8:06 0.0 8:36 0.0 11:07 0.0 11:37 0.0 7:40 8:18 0.0 8:48 0.0 11:19 0.0 11:49 0.0 8:04 8:41 0.0 9:12 0.0 11:42 0.0 12:12 0.0 7:35 8:13 0.0 8:43 0.0 7:56 11:14 8:33 0.0 0.0 11:44 9:03 0.0 0.0 11:34 0.0 12:04 0.0 7:59 8:36 0.0 9:06 0.0 11:37 0.0 12:07 0.0 7:46 8:24 0.0 8:54 0.0 11:25 0.0 11:55 0.0 8:21 8:58 0.0 9:28 0.0 11:59 0.0 12:29 0.0 ____ TIME 10:19 13:20 10:27 13:28 10:03 13:04 10:03 13:04 10:07 13:08 10:04 13:04 9:06 12:07 9:18 12:19 9:42 12:43 9:13 12:14 9:34 12:34 9:36 12:37 9:24 12:25 9:59 12:59 INDIVIDUAL DATA CONC. TIME 0.0 10:49 0.0 13:50 0.0 10:58 0.0 13:58 0.0 10:33 0.0 13:34 0.0 10:33 0.0 0.0 13:34 10:37 0.0 13:38 0.0 0.0 10:34 13:35 0.0 9:37 0.0 12:37 0.0 9:48 0.0 12:49 0.0 10:12 0.0 13:13 0.0 9:43 0.0 12:44 0.0 10:04 0.0 13:05 0.0 0.0 10:07 13:07 0.0 9:54 0.0 12:55 0.0 10:29 0.0 13:30 CONC . 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 TIME 11:19 14:20 11:28 14:28 11:03 14:04 11:04 14:04 11:07 14:08 11:04 14:05 10:07 13:07 10:19 13:19 10:42 13:43 10:13 13:14 10:34 13:35 10:37 13:38 10:24 13:25 10:59 14:00 CONC. 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 TIME CONC. MEAN S.D. 11:50 0.0 0.0 0.0 11:58 0.0 0.0 0.0 11:34 0.0 0.0 0.0 11:34 0.0 0.0 0.0 11:37 0.0 0.0 0.0 11:34 10:37 10:49 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 11:12 10:43 11:04 11:07 10:54 11:29 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 N 11 11 11 11 11 11 11 11 11 11 11 1) 11 11 I cn r* owo CcOn 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Study ID: K-001716-091 Volume 2 of 3, Page: 14 Appendix B, continued: Chamber Analyses ANALYTICAL AND NOMINAL CONCENTRATION - CONTROL (CONT.J DATE 16-Aug-90 17-Aug-90 20-Aug-90 21-Aug-90 22-Aug-90 23-Aug-90 24-Aug-90 27-Aug-90 2B-Aug-90 29-Aug-90 30-Aug-90 31-Aug-90 EXPOSURE ____________________________________ BEGAN TIME CQNC. TIME CONC. TIME 7:36 8 13 0.0 8:43 0.0 9 13 7:34 U 14 8 11 0.0 0.0 11:44 8:41 0.0 0.0 12 14 9 11 11 12 0.0 11:42 0.0 12 12 8:22 9 00 0.0 9:30 0.0 10 00 12 01 0.0 12:31 0.0 13 01 8:00 8 37 0.0 9:07 0.0 9 38 11 38 0.0 12:08 0.0 12 38 8:30 9 08 0.0 9:38 0.0 10 08 12 09 0.0. 12:39 0.0 13 09 8:15 8 52 11 53 0.0 0.0 9:22 12:23 0.0 0.0 9 52 12 53 8:14 a 51 0.0 9:21 0.0 9 52 11 52 0.0 12:22 0.0 12 52 8:24 9 02 0.0 9:32 0.0 10 02 12 02 0.0 12:32 0.0 13 03 8:38 9 15 12 16 0.0 0.0 9:45 12:46 0.0 0.0 10 15 13 16 8:23 9 01 0.0 9:31 0.0 10 01 12 02 0.0 12:32 0.0 13 02 8:23 9 00 0.0 9:30 0.0 10 00 12 01 0.0 12:31 0.0 13 01 8:19 8 57 11 58 0.0 0.0 9:27 12:28 0.0 0.0 9 57 12 58 INDIVIDUAL DATA CONC. TIME 0.0 9:43 0.0 0.0 12:44 9:41 0.0 0.0 .12:42 10:30 0.0 13:31 0.0 10:08 0.0 0.0 13:08 10:38 0.0 13:39 0.0 0.0 10:23 13:23 0.0 10:22 0.0 13:23 0.0 10:32 0.0 13:33 0.0 10:45 0.0 13:46 0.0 10:31 0.0 0.0 13:32 10:30 0.0 13:31 0.0 0.0 10:27 13:28 CONC. 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 TIME 10:14 13:14 10:12 13:12 11:01 14:01 10:38 13:39 11:08 14:09 10:53 13:53 10:52 13:53 11:02 14:03 11:16 14:16 11:01 14:02 11:01 14:01 10:57 13:58 CONC. 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 TIME CONC. 10:44 0.0 10:42 0.0 11:31 0.0 11:08 0.0 11:38 0.0 11:23 0.0 11:22 0.0 11:32 0.0 11:46 0.0 11:31 0.0 11:31 0.0 11:27 0.0 N= MEAN T M.A. S.D. RANGE MEAN 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 68 0.0 0 0 S.D. 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0 N 11 11 11 11 11 11 11 11 11 11 11 11 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Study ID: K-001716-091 Volume 2 of 3, Page: 15 O) Appendix B, continued: Chamber Analyses OCo ANALYTICAL AND NOMINAL CONCENTRATION - LOW DOSE DATE 2 9-May-90 30-May-90 31-May-90 l-Jun-90 4-Jun-90 5-Jun-90 6-Jun-9G 7-Jun-90 8-Jun-90 1l-Jun-90 12-Jun-91 13-Jun-90 14-Jun-90 15-Jun-90 EXPOSURE ___________ INDIVIDUAL DATA BEGAN TIME CONC. TIME CONC, TIME CONC. TIME CONC. TIME CONC. TIME CONC. MEAN S.D. N NOMINAL 8:43 7:44 9:29 12:30 9:29 12:31 217.3 208.8 219.1 201.8 9:59 13:00 10:00 13:01 212.7 209.7 217.9 202.9 10:29 13:31 10:30 13:32 215.8 207.5 209.4 200.0 10:59 14:01 11:00 14:02 209.7 208.1 205.3 197.8 11: 30 14:31 11:30 14 : 32 209.0 207.8 204.6 198.7 12:00 12:01 211.1 210.7 2 02.9 205.5 3.3 7.2 11 11 226.8 2 18.1 9: OS 9:51 209.5 10:21 208.1 10:51 12:52 205.2 13:22 202.8 13:53 207.9 204.1 11:21 14:23 209.1 201.7 11:52 14:53 208.2 201.2 12:22 205.2 205.7 3.0 11 220.1 8:43 9:00 9:28 12:30 9:48 211.5 205.9 211.7 9:58 13:00 10:18 210.1 202.7 210.5 10:29 13:30 10:48 209.6 206.6 210.6 10:59 14:00 11:18 210.2 205.1 212.5 11:29 14:31 11:49 210.8 204.9 210.5 11:59 12:19 208.1 207.8 210.3 209.0 2.9 3.0 11 11 222.8 224.4 12:49 210,3 13:20 208.5 13:50 204.5 14:20 204.1 14:51 205.1 8:57 9:42 216.7 10:12 216.1 10:43 12:44 206.7 13:14 206.6 13:44 210.3 205.4 11:13 208.2 11:43 208.6 12:13 207.8 I 90.1 63.2 14:14 204.7 0:00 0.0 11 2 31.7 9:00 8:47 9:44 12:45 9:34 12:36 197.9 200.7 206.2 207.1 10:14 13:16 10:05 13:06 198.1 201.0 209.5 206.6 10:44 13:46 10:35 13:37 198.3 200.0 209.2 206.1 11:15 14:16 11:05 14:07 200.4 198.5 207.5 196.2 11:45 14:47 11:36 14:37 202.7 200.7 206.0 203.5 12:15 198.6 199.7 12:06 206.4 205.8 1 .6 3.6 11 11 222.5 224 . 4 8:43 9:29 213.0 9:59 214.4 10:29 12:30 213.3 13:00 211.8 13:31 212.2 212.2 10:59 14:01 211.8 214.9 11:30 14:31 211.1 207.5 12:00 212.3 212.2 1.9 11 225.0 8:45 8:36 9:31 12:37 9:21 12:23 195.6 191.2 208.7 206.6 10:02 13:08 9:51 12:53 192.9 188.5 207.4 204.5 10:33 13:39 10:22 13:23 192.5 187.8 208.6 206.1 11:04 14:10 10:52 13:54 192.9 186.7 204.6 203.3 11:35 14:41 11:22 14:24 193.4 186.8 207.9 204.8 12:06 11:53 192.1 190.9 207.6 206.4 3.0 1 .9 11 11 205.6 218.6 8:46 9:31 12:33 215.6 212.1 10:02 13:03 213.5 212.1 10:32 13:33 210.1 210.4 11:02 14:04 199.2 208.5 11:32 14:34 212.1 201.8 12:03 210.3 209.6 4.9 )1 222.7 8:46 8:48 9:31 12:33 9:33 12:34 210.9 204.9 210.0 208.2 10:01 13:03 10:03 13:04 207.8 204.6 208.9 204.2 10:32 13:33 10:33 13:35 207.3 203.7 206.7 205.1 11:02 14:04 11:03 14:05 206.4 202.6 207.4 205.1 11:32 14:34 11:34 14:35 206.4 206.1 204.8 205.2 12:03 207.2 206.2 12:04 204.9 206.4 2.3 2.0 )1 11 224.7 225.8 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Study ID: K-001716-091 Volume 2 of 3, Page: 16 Appendix B, continued: Chamber Analyses ANALYTICAL AND NOMINAL CONCENTRATION - LOW DOSE (CONT.l DATE 18-Jun-90 19-Jun-90 20-Jun-90 21-Jun-90 22-Jun-90 2 5 -J u n - 90 26-Jun-90 27-Jun-90 28-Jun-90 29-Jun-90 2-Jul-90 3-Jul-90 5-Jul-90 6-Jul-90 INDIVIDUAL EXPOSURE_______________________________________________ DATA BEGAN TIME CONC. TIME CONC. TIME CONC. TIME B: 43 8:37 9:28 12:30 9:22 205.2 202.2 212.7 9:58 13:00 9:53 204.6 199.7 213.1 10:29 13:30 10:23 203.3 198.8 207.2 10:59 14:01 10:53 8:36 8:37 8:38 8:48 12:24 9:21 12:23 9:22 12:24 9:24 12:25 9:33 12:35 208.3 211.7 207.5 213.0 208.1 211.0 203 r 8 212.5 211.8 12:54 9:51 12:53 9:53 12:54 9:54 12:55 10:03 13:05 210.5 208.6 207.7 209.3 204.2 208.9 207.1 217.0 211.7 13:24 10:21 13:23 10:23 13:25 10:24 13:26 10:34 13:35 207.6 210.3 208.3 208.7 208.8 206.6 208.0 213.7 210.2 13:55 10:52 13:53 10:53 13:55 10:54 13:56 11:04 14:06 8:23 8:30 9:08 12:09 9:20 208.5 206.9 215.5 9:38 12:40 9:50 208.6 206.9 215.9 10:08 13:10 10:21 208.5 207.3 215.3 10:39 13:40 10:51 8:38 12:22 9:24 12:25 211.9 215.4 209.9 12:52 9:54 12:55 208.2 213.1 209.8 13:22 10:24 13:26 212.2 210.7 209.3 13:52 10:54 13:56 8:30 8:32 9:15 12:16 9:17 216.1 N.D. 214.0 9:45 12:47 9:47 213.2 N.D. 212.5 10:15 13:17 10:17 213.9 210.6 213.0 10:46 13:47 10:48 8: 4B 12:19 9:34 12:35 208.7 214.3 210.3 12:49 10:04 13:06 210.9 212.6 N.D. 13:19 10:34 13:36 209.7 211.5 N.D. 13:49 11:04 14:06 8:40 8:34 9:24 12:26 9:19 214.7 213.2 215.2 9:55: 12:56 9:49 213.5 210.7 215.0 10:25 13:26 10:19 214.1 210.3 214.7 10:55 13:57 10:50 12:21 213.2 12:51 210.2 13:21 210.0 13:51 CONC. 205.6 201.7 209.4 202.5 210.6 209.9 210.7 209.3 209.9 207.0 214.7 211.8 208.0 205.3 213.7 209.1 210.0 209.9 214.8 210.0 212.9 207.4 213.6 N.D. 212.7 210.3 212.0 210.4 TIME 11:29 14:31 11:24 14:25 11:22 14:24 11:23 14:25 11:24 14:26 11:34 14:36 11:09 14:10 11:21 14:23 11:25 14:26 11:16 14:17 11:18 14:20 11:35 14:36 11:25 14:27 11:20 14:22 CONC. 203.2 197.9 208.9 203.4 212.1 206.3 209.5 208.5 208.6 205.1 213.3 210.5 207.0 202.6 211.2 210.5 210.8 208.2 211.2 N.D. 210.1 207.4 N.D. 209.7 212.0 208.7 213.8 209.3 TIME 11:59 11:54 11:52 11:54 11:55 12:05 11:39 11:51 11:55 11:46 11:48 12:05 11:56 11:50 CONC. MEAN 202.6 202.3 210.3 208.5 209.7 209. 3 208.7 209.0 205.8 207.4 211 .5 212.6 208.4 207.1 N.D. 212.4 210.1 210.7 N.D. 212.8 N.D. 210.7 208.3 211.5 213.7 212.2 211.7 212.3 S.D. 2.6 3.3 1.8 2.1 2.1 2.0 1.8 2.7 2.0 2.3 2.4 2.2 1.9 2.2 N NOMINAL 11 218.3 11 223 . 1 11 227.1 11 227.9 11 225.6 11 22 ) .0 11 2 2 4.7 10 224 .1 11 223.3 1 226.8 10 223.1 7 220.8 11 226.1 11 230.0 SL 030853 1,1,1-T subchronic neurotoxicity f Appendix B, continued; Chamber Analyses The Dow Chemical Company Study ID; K-001716-091 Volume 2 of 3, Page; 17 030854 ANALYTICAL AND NOMINAL CONCENTRATION - LOW DOSE (CONT.) DATE 9-Jul-90 10-Jul-90 ll-Jul-90 12-Jul-90 13-Ju1-90 16-Jul-90 n-Jui-90 ie-Jui-90 19-Ju1-90 20-Jul-90 23-Jul-90 2 4 -J u 1 - 90 25-Jul-90 26-Jul-90 EXPOSURE _____ ________________________ DATA BEGAN TIME CONC. TIME CONC. TIME 6:33 9:18 217.1 9:49 214.6 10 19 6:52 12:20 9:35 212.6 207.3 12:50 10:05 210.6 206.9 13 21 10 36 12:36 205.4 13:07 N.D. 13 37 8:30 9:15 206.8 9:45 208.7 10 15 12:16 204.3 12:47 204.5 13 17 8:45 6:37 9:30 12:30 9:21 212.0 206.3 216.3 10:00 13:00 9:51 210. 1 206.6 213.2 10 30 13 31 10 21 8:52 12:22 9:37 12:38 210,4 206.9 203.4 12:52 10:07 13:06 208.7 207.7 205.7 13 22 10 36 13 38 6:56 8:20 9:43 12:44 9:05 212.1 209.1 214.4 10:13 13:14 9:35 212.7 209.7 214.1 10 43 13 44 10 06 8:45 8:42 12:06 9:30 12:31 9:27 211.2 214.2 212.2 209.5 12:36 10:00 13:01 9:57 211.9 214.1 214.2 211.1 13 06 10 30 13 31 10 27 8:36 12:27 206.6 12:57 206.9 13 28 9:21 214.0 9:51 211.9 10 21 12:22 211.2 12:52 209.3 13 22 6:46 8:16 9:33 12:34 9:01 216.0 211.3 209.0 10:03 13:04 9:31 214.1 210.7 210.0 10 34 13 34 10 02 8:44 12:02 9:29 12:30 208.5 209.2 207.7 12:32 9:59 13:00 208.6 209.7 207.4 13 02 10 29 13 30 INDIVIDUAL CONC. 213.8 210.0 206.5 N.D. 208.6 203.8 208.8 206.0 211.9 209.5 209.2 204.2 211.9 209.3 211.9 208.3 211.6 211.0 207.6 207.0 210.0 209.4 211.2 211.2 209.4 206.7 209.5 207.3 TIME 10:49 13:51 11:06 .14:07 10:46 13:47 11:00 14:01 10:51 13:52 11:08 14:08 11:14 14:14 10:36 13:37 11:00 14:01 10:57 13:58 10:51 13:52 11:04 14:05 10:32 13:33 11:00 14:00 CONC. 214.5 209.3 208.2 N.D. 205.5 202.2 208.1 206.0 211.0 208.7 208.1 203.1 211.9 209.5 212.3 209.7 214.6 211.1 206.9 205.4 210.0 209.6 212.8 209.1 207.4 207.9 210.0 207.3 TIHE 11:19 14:21 11:36 14:38 11:16 14:18 11:30 14:31 11:21 14:22 11:38 14:39 11:44 14:45 11:06 14:07 11:30 14:31 11:27 14:28 11:21 14:22 11:34 14:35 11:02 14:03 11:30 14:30 CONC. 213.7 208.2 206.8 204.1 205.0 204.1 209.1 204.6 210.6 206.6 204.6 203.1 211.9 207 .B 211.9 207.7 212 .8 207.1 207.3 205.2 211.9 209.6 213.7 208.8 208.9 207.0 209.0 206.0 TIME 11:50 12:06 11:46 12:00 11:52 12:08 12:14 11:36 12:00 11:57 11:52 12:04 11:32 12:00 CONC. MEAN S.D. 211.9 212.4 2.7 204.5 206.2 1 .4 205.3 205.5 2.2 208.8 207.8 2.2 210.9 210.7 2.5 202.7 205.5 2.5 211.2 210.6 1.6 211.0 211.3 2.1 212.5 212.3 2.2 207.1 207.3 1 .7 210.3 210.7 1 . 5 212.1 211.9 2 . 1 208.2 208.3 1.0 209.4 208.4 1 . 3 N NOMINAL 11 223.7 8 223.6 11 222.2 11 224.7 11 228.2 11 232.8 It 225.5 11 22 3.5 11 22 3.5 11 224.0 11 223.2 11 227.5 11 226.0 1 1 222.7 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Study ID: K-001716D91 Volume 2 of 3, Page: 18 Appendix B, continued: Chamber Analyses ANALYTICAL AND NOMINAL CONCENTRATION - LOW DOSE (CONT.I DATE 27-Jul-90 30-Jul-90 31-Jul-90 l-Aug-90 2-Aug-90 3-Aug-90 6-Aug-90 7-Aug-90 8-Aug-90 9-Aug-90 10-Aug-90 13-Aug-90 H-Aug-90 15-Aug-90 INDIVIDUAL EXPOSURE ___________________ DATA BEGAN TIME CONC. TIME CONC. TIME CONC. TIME CONC. TIME CONC. TIME CONC. MEAN S.D. N NOMINAL 8:41 9:26 208.9 9:56 208.4 10:27 12:27 206.8 12:57 207.3 13:27 206.9 207.0 10:57 208.0 11:27 206.9 11:57 13:58 205.8 14:28 206.6 208.6 207.4 0.9 11 227.8 8:50 9:35 201.1 10:05 202.9 10:35 12:35 200.1 13:06 198.5 13:36 202.1 199.1 11:05 200.9 11:35 199.7 12:05 202.7 200.4 14:06 198.4 14:36 198.4 1.7 ]1 219.4 8:25 9:10 12:11 N.D. 213.7 9:41 12:41 N.D. 211.3 10:11 13:11 213.5 211.4 10:41 212.8 11:11 211.3 11:41 13:42 212.4 14:12 213.0 213.4 212.5 1.0 9 227.7 8:25 8:29 9:10 12:11 9:14 12:15 212.7 211.7 216.3 216,8 9:41 12:41 9:44 12:45 213.7 N.D. 214.5 211.8 10:11 13:12 10:14 13:15 N.D. 212.4 217.6 214.5 10:41 13:42 10:44 13:45 211.5 209.4 212.6 211.0 11:11 14:12 11:15 14:15 210.5 N.D. 214.8 213.2 11:41 11:45 209.9 211.5 218.8 214.7 1.5 2.5 8 11 225.4 223.2 8:26 9:11 211.3 9:41 214.7 10:11 12:12 212.9 12:42 214.5 13:12 210.5 209.7 10:41 211.1 11:11 214.6 11:42 215.1 212.4 13:42 210.5 14:12 211.4 2.0 11 225.0 7:28 8:14 216.2 8:44 11:14 213.5 11:45 214.9 9:14 213.9 12:15 213.7 212.6 9:44 12:45 212.0 213.6 10:14 13:15 210.6 210.3 10:44 213.1 213.1 1 .7 11 222.3 7:40 8:25 212.5 8:55 212.1 9:26 11:26 209.6 11:56 209.5 12:27 209.0 209.4 9:56 212.1 12:57 208.5 10:26 210.5 10:56 209.9 210.3 13:27 210.2 1.4 11 226.2 8:04 8:49 225.8 9:19 225.4 9:49 11:50 222.4 12:20 218.8 12:50 221.4 214.3 10:19 219.9 10:50 222.5 11:20 220.9 219.9 4 . 4 13:20 213.8 13:50 213.4 11 2 32.8 7:35 8:20 11:21 212.4 209.7 8:50 11:51 217.4 209.8 9:21 12:21 216.2 207.2 9:51 12:51 214.1 210.4 10:21 211.3 13:22 207.0 10:51 212.8 211.7 3.3 11 22 1.4 7:56 8:41 N.D. 9:11 212.2 9:41 217.2 10:11 215.8 10:41 211.1 11:12 211.3 211.9 2.9 10 225. 1 11:42 213.0 12:12 212.0 12:42 208.6 13:12 208.7 13:42 209.1 7:59 8:44 211.4 9:14 211.3 9:44 11:44 207.3 12:15 207.1 12:45 203.9 208.4 10:14 207.6 10:44 209.1 13:15 207.3 13:45 208.4 11:14 209.7 208.3 2.1 11 22 3.5 7:46 8:31 11:32 212.0 209.1 9:02 12:02 211.2 207.7 9:32 12:32 209.0 205.9 10:02 209.2 10:32 206.7 11:02 207.6 208.3 2.0 13:03 206.0 13:33 207.0 11 223.2 8:21 9:06 213.8 9:36 215.9 10:06 12:07 208.4 12:37 210.1 13:07 213.8 213.5 10:36 13:37 213.0 209.4 11:06 14:07 212.1 210.6 11:36 210.0 211.9 2.3 11 225.1 SL 030855 SL 030856 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Study ID: K-001716-091 Volume 2 of 3, Page; 19 Appendix B, continued: Chamber Analyses ANALYTICAL AND NOMINAL CONCENTRATION - LOW DOSE (CONT.) EXPOSURE DATA DATEBEGAN TIME CONC. I6-Aug-90 17-Aug-90 7:36 7:34 8 21 11 21 6 19 11 19 215.0 209.3 217.0 215.7 20-Aug-90 21-Aug-90 6:22 8:00 9 08 12 06 8 45 216.6 214.2 211.1 22-Aug-90 8:30 11 46 207.6 9 15 211.3 12 16 208.2 23-Aug-90 24-Aug-90 6:15 8:14 9 00 12 00 8 59 216.0 216.7 217.1 27-Aug-90 28-Aug-90 8:24 8:38 12 00 9 09 12 10 9 23 12 23 213.6 203.0 205.5 212.4 209.1 29-Aug-90 8:23 9 08 213.0 30-Aug-9Q 31-Aug-90 8:23 8:19 12 09 9 07 12 08 9 04 12 05 209.2 214.2 212.3 206.4 199.5 TIME 8:51 11:51 8:49 11:50 9:38 12:39 9:15 12:16 9:45 12:46 9:30 12:31 9:29 12:30 9:39 12:40 9:53 12:54 9:36 12:39 9:38 12:39 9:34 12:35 CONC. 213.0 204.7 212.6 209.7 214.2 214.3 214.2 208.7 210.8 206.3 210.8 213.9 220.4 215.5 201.4 201.8 213.5 210.0 209.8 210.8 214.5 212.8 203.6 203.2 TIME 9 21 12 22 9 19 12 20 10 08 13 09 9 45 12 46 10 16 13 16 10 00 13 01 9 59 13 00 10 09 13 10 10 23 13 24 10 09 13 10 10 08 13 09 10 04 13 05 INDIVIDUAL CONC. 212.9 207.0 218.0 213.2 212.3 214.0 209.8 207.5 211.3 207.9 N.D. 214.6 215.7 215.0 207.3 200.0 208.0 210.5 209.1 209.3 213.3 210.5 206.8 201.2 TIME 9:51 12:52 9:49 12:50 10:38 13:39 10:15 13:16 10:46 13:46 10:30 13:31 10:29 13:30 10:39 13:40 10:53 13:54 10:39 13:40 10:38 13:39 10:35 13:35 CONC. 210.8 208.6 214.5 209.9 213.0 211.7 211.9 210.7 209.8 206.3 255.2 215.6 217.7 211.6 199.8 203.8 212.3 202.7 210.9 210.5 212.8 211.6 202.8 200.1 TIME 10:21 13:22 10:19 13:20 11:08 14:09 10:45 13:46 11:16 14:17 11:00 14:01 10:59 14:00 11:10 14:10 11:23 14:24 11:09 14:10 11:08 14:09 11:05 14:06 _____________________________________________________________ CONC. TIHE CONC. MEAN S.D. N NOMINAL 213.0 203.6 213.4 213.5 10:51 10:49 213.8 210.2 213.7 213.8 3.8 2.6 11 11 224.4 230.2 213.8 11:38 215.3 213.6 1.7 11 226.5 210.6 210.8 207.2 210.7 210.3 213.1 11:15 11:46 11:30 209.2 209.9 207.9 209.2 211.8 218.2 2.1 3 .9 13.2 11 11 10 226. / 225.2 225.9 212.6 214.0 208.3 201.2 11:30 11:40 211.3 214.6 N.D. 202.4 3.4 2.5 11 10 225.0 210.7 200.4 214.4 202.5 210.7 206.9 212.1 11:53 11:39 11:38 210.7 209.7 210.7 210.1 211.8 212.2 4.0 1.5 1.7 11 11 11 220.3 2 31.7 233 .1 208.6 204.0 11:35 202.4 202.7 2.4 11 222.0 200.0 NMEAN T . W. A. S.D. RANGE 68 209.0 4.893 190.1 220 224 .4 4.363 205.6 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Study ID: K-001716-091 Volume 2 of 3, Page: 20 Appendix B, continued: Chamber Analyses ANALYTICAL AND NOMINAL CONCENTRATION - MIDDLE DOSE DATE 29-May-90 30-May-90 31-May-90 I-Jun-90 4-Jun-90 5-Jun-90 6-Jun-90 7-Jun-90 8-Jun-90 11-Jun-90 12-Jun-91 13-Jun-90 H-Jun-90 15-Jun-90 EXPOSURE BEGAN TIME 8:43 3:44 9:36 12:38 9:33 12:38 9:05 9:58 13:00 8:43 9:36 9:00 12:33 9:55 12:53 8:53 9:00 9:50 12:51 9:51 8:43 8:43 12:53 9:42 12:44 9:36 12:38 8:45 8:36 9:39 12:45 9:29 12:30 8:46 9:39 12:40 8:46 8:48 9:39 12:40 9:40 12:42 CONC. 634.8 621.3 641.3 630.6 652.3 621.9 638.2 612.3 611.2 620.5 634.9 635.2 640.3 634.4 643.3 633.6 643.6 633.2 650.3 643.2 633.3 623.2 649.9 635.3 622.6 618.2 632.4 621.5 _____ TIME 10:06 13:08 10:03 13:09 10:28 13:30 10:06 13:03 10:25 13:23 10:20 13:21 10:22 13:23 10:12 13:14 10:06 13:08 10:10 13:16 9:59 13:01 10:09 13:11 10:09 13:11 10:10 13:12 INDIVIDUAL __________________________ DATA CONC. TIME CONC. TIME 633.9 10:37 637.2 11:07 620.3 13:38 619.4 14:09 638.7 10:37 637.6 11:08 635.4 13:39 628.7 14:09 638.5 10:59 629.8 11:29 626.9 14:00 622.9 14:31 635.2 10:36 625.0 11:06 610.5 13:38 611.6 14:08 610.0 10:56 625.3 11:26 620.2 13:57 604.6 14:28 646.6 10:50 639.5 11:20 634.5 13:52 6.3 14:22 640.8 10:52 642.8 11:22 635.2 13:54 630.0 14:24 646.0 10:42 644.8 11:13 636.3 13:44 633.9 14:14 647.5 10:37 645.8 11:07 637.9 13:38 634.6 14:08 646.5 10:41 644.9 11:12 639.0 13:47 641.2 14:18 638.8 10:29 631.6 10:59 624.0 13:31 620.6 14:01 642.9 10:39 N.D. 11:10 635.5 13:41 631.0 14:11 622.0 10:39 624.3 11:10 616.4 13:41 612.4 14:11 627.4 10:41 625.4 11:11 618.9 13:42 621.3 14:13 CONC. 633.4 618.2 633.5 624.6 631.4 620.5 625.9 613.4 630.7 599.8 641.4 602.6 641.4 629.2 645.5 633.3 642.7 640.3 642.1 637.6 629.6 621.0 284.7 615.8 620.9 615.1 624.5 618.7 TIME 11:37 14:39 11:38 14:39 11:59 15:01 11:37 14:38 11:56 14:58 11:51 11 :S2 11:43 14:45 11:37 14:39 11:43 14:49 11:30 14:31 11:40 14:41 11:40 14:41 11:41 14:43 CONC. 630.6 618.9 632.0 625.8 629.1 621.6 620.9 610.8 628.8 648.5 640.3 638.1 641.2 59.6 64 3.5 632.0 645.0 594.7 634.9 621.2 638.2 75.6 621.4 614.4 619.2 620.3 TIME 12:07 12:08 12:30 12:03 12:26 12:21 12:23 12:13 12:07 12:14 12:00 12:10 12:10 12:11 CONC. MEAN S.D. 632.0 627.3 7.5 632.2 632.8 5.3 624.2 629.0 9.4 615.9 620.0 9. 9 627.3 620.6 13.8 639.4 576.1 201.0 638.5 637.1 4.8 639.3 587.7 175.2 639.9 640.5 4.8 642.9 636.9 15.0 630.4 628.0 6.4 632.5 544 .2 198.3 619.4 618.8 3.8 620.9 622.8 4 . 3 N NOMINAL 11 651.1 ) 1 653. 3 11 652.2 11 648.2 11 648.8 10 601.2 10 668.0 11 635.2 11 669.0 11 667 .7 11 662.6 10 634.8 11 671.3 11 668.8 SL 030857 1,1,1-T subchronic neurotoxicity rji The Dow Chemical Company Study ID: K-001716-091 Volume 2 of 3, Page: 21 030858 Appendix B, continued: Chamber Analyses ANALYTICAL AND NOMINAL CONCENTRATION - MIDDLE DOSE (CONT.) DATE 18-Jun-90 19-Jun-90 20-Jun-90 21-Jun-90 22-Jun-90 25-Jun-90 26-Jun-9Q 27-Jun-90 28-Jun-90 29-Jun-90 2-JuI-90 3-Jui-90 5-Jul-90 6-jui-9o EXPOSURE _________________________ BEGAN TIME CONC. TIME CONC. 8:43 9:36 629.1 10:06 628.3 8:37 12:37 622.1 13:08 618.9 9:30 641.2 10:00 636.3 12:31 627.2 13:02 625.8 6:36 9:28 628.7 9:59 629.4 12:30 617.8 13:00 616.3 6:37 9:30 636.4 10:00 631.3 8:36 12:32 612.5 13:02 611.3 9:31 622.4 10:01 617.9 12:33 610.f 13:03 609.9 8:48 9:41 641.9 10:11 646.0 12:42 622.5 13:13 619.9 6:23 9:15 635.6 9:46 633.9 8:30 12:17 622.4 12:47 623.3 9:28 646.0 9:58 644.7 12:29 625.8 12:59 622.0 8:38 8:30 9:31 12:33 9:22 635.5 619.7 636.7 10:01 13:03 9: S3 628.5 619.5 632.0 8:32 12:24 N.D. 12:54 N.D. 9:24 638.9 9:55 631.9 12:26 612.5 12:56 610.8 8:46 9:41 629.9 10:11 625.4 12:43 613.7 13:13 N.D. 8:40 9:32 633.5 10:02 629.8 8:34 12:33 618.9 13:04 614.7 9:26 621.0 9:57 619.3 12:28 606.6 12:58 606.6 INDIVIDUAL _______ DATA TIME CONC. TIME 10:36 626.0 11:06 13:38 617.9 14:08 10:30 630.8 11:01 13:32 625.1 14:02 10:29 625.7 10:59 13:31 613.3 14:01 10:31 631.1 11:01 13:32 10:31 608.9 620.9 14:02 11:02 13:33 618.8 14:04 10:41 13:43 640.2 618.7 11:12 14:13 10:16 628.6 10:46 13:18 617.0 13:48 10:28 641.0 10:58 13:30 620.8 14:00 10:32 626.3 11:02 13:33 618.5 14:04 10:23 630.0 10:53 13:24 619.4 13:55 10:25 628.1 10:55 13:27 605.7 13:57 10:42 621.2 11:12 13:43 N.D. 14:14 10:32 627.4 11:03 13:34 610.4 14:04 10:27 617.2 10:57 13:29 604.8 13:59 CONC. 628.2 616.1 629.1 116.8 623.6 611.4 626.5 610.1 622.5 613.2 636.1 616.3 628.9 617.1 628.5 622.1 626.5 617.0 631.6 621.6 628.5 604.2 619.3 N.D. 620.5 606.2 611.0 601.6 TIME 11 37 14 38 11 31 14 33 11 30 14 31 11 31 14 33 11 32 14 34 11 42 14 43 11 16 14 18 11 29 14 30 11 32 14 34 11 23 14 25 11 26 14 27 11 42 14 44 11 33 14 35 11 28 14 29 CONC. 623.6 613.7 625.7 0.5 624.4 609.5 621.0 608.7 617.1 611.5 633.7 614.9 629.6 617.2 627.7 619.0 626.5 613.7 626.4 N.D. 630.2 601.6 N.D. 605.4 620.0 598.3 609.4 604.1 TIME 12:07 12:01 12:00 12:01 12:02 12:12 11:47 11:59 12:02 11:54 11:56 12:13 12:03 11:58 CONC. MEAN S.D. 625.4 622.6 5.3 630.6 526.3 232.7 620.6 620,1 6.9 622.2 620.0 10.2 614.1 616.2 4.7 629.7 62 9.1 11.2 627.5 62 5.6 6. 6 N.D. 629.8 10.3 625.9 62 3.4 6.3 N.D. 62 B, 3 6.1 N.D. 619.2 13.6 613.4 618.3 8.2 618.1 618.0 10.4 608.6 610.0 6.5 N NOMINAL 11 657.2 11 5 92.1 11 669.4 11 668.0 11 662.6 11 663.2 11 664.0 10 661.3 11 659.8 7 659.8 10 657.0 7 64 9.2 1 1 654.3 11 659.6 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Study ID: K-001716-091 Volume 2 of 3, Page: 22 Appendix B, continued: Chamber Analyses ANALYTICAL AND NOMINAL CONCENTRATION - MIDDLE DOSE (CONT.) DATE 9-Jul-90 10-Jui-90 11-Jul-90 12-JuI-90 13-Jul-90 16-Jul-90 17-Jul-90 18-Jul-90 19-Jul-90 20-Jul-90 23-Jul-90 24-Jul-90 25-Jul-90 26-JU1-90 INDIVIDUAL EXPOSURE_________________________________________ DATA BEGAN TIME CONC. TIME CONC. TIME CONC. TIME 8:33 8:52 8:30 8:45 8:37 8:52 8:58 8:20 8:45 8:42 8:36 8:48 8:16 8:44 9:26 12:28 9:43 12:44 9:23 12:24 9:31 12:38 9:28 12:29 9:45 12:46 9:51 12:52 9:13 12:14 9:37 12:38 9:34 12:35 9:28 12:29 9:41 12:42 9:09 12:10 9:37 12:37 627.1 614.2 630.7 616.3 632.3 612.2 624.8 611.1 640.3 619. ? 635.5 619.7 638.0 629.4 650.1 636.4 642.3 631.9 641.2 625.1 643.8 634.7 653.9 630.0 642.5 621.2 642.2 627.3 9 56 12 58 10 13 13 14 9 53 12 54 10 07 13 08 9 59 12 59 10 15 13 16 10 21 13 22 9 43 12 44 10 07 13 08 10 04 13 05 9 58 12 59 10 11 13 12 9 39 12 40 10 07 13 08 624.9 615.1 629.6 N.D. 632.3 608.3 621.6 605.7 636.4 616.8 626.0 619.1 638.3 626.9 645.5 633.7 645.7 635.6 640.1 625.7 640.9 633.6 650.4 626.8 644.6 621.4 635.1 623.2 10:26 13:2B 10:43 13:45 10:23 13:25 10:37 13:38 10:29 13:29 10:45 13:46 10:51 13:52 10:13 13:14 10:38 13:38 10:34 13:35 10:29 13:29 10:41 13:42 10:09 13:10 10:37 13:38 625.2 612.0 625.1 N.D. 630.9 606.7 616.2 604.3 629.9 612.1 632.0 613.9 637.8 623.4 640.6 631.9 641.8 628.0 634.2 619.0 633.3 634.2 645.3 624.3 641.7 620.3 632.6 617.2 10:57 13:58 11:13 14:15 10:53 13:55 11:07 14:08 10:59 14:00 11:15 14:16 11:21 14:22 10:43 13:44 11:08 14:09 11:04 14:05 10:59 14:00 11:11 14:12 10:39 13:40 11:07 14:08 CONC. 624.0 608.1 626.8 N.D. 622.9 607.8 614.7 601.8 628.7 611.0 627.0 610.5 636.5 619.1 642.1 626.1 638.0 623.6 636.1 620.7 630.1 634.7 644.4 621.7 636.3 620.7 633.5 618.7 TIME 11 27 14 29 11 43 14 45 11 23 14 25 11 38 14 38 11 29 14 30 11 45 14 46 11 51 14 52 11 13 14 14 11 38 14 39 11 35 14 35 11 29 14 30 11 41 14 42 11 09 14 10 11 37 14 38 CONC 620.3 605.9 622.5 602.5 614.8 604.3 614.1 602.1 625.3 605.8 622.8 610.2 635.9 615.9 640.0 620.1 637.0 620.1 633.9 615.0 634.4 628.9 641.6 619.5 630.7 613.1 628.0 615.1 TIHE 11:57 12:14 11:54 12:08 11 :59 12:15 12:21 11:44 12:08 12:05 11:59 12:12 11:40 12:07 CONC. MEAN 618.1 617.7 619.2 621.6 616.6 617.2 613.2 611.8 620.5 622.4 621.8 621.7 633.8 630.5 637.6 636.7 635.2 634.5 629.6 629.1 636.1 635.0 634.1 635.6 626.6 629.0 626.6 627.2 S.D. 7.3 9.1 10.7 7.7 10.8 8.2 8.1 8.6 7.9 8.8 4.3 12.0 10.8 8.3 N NOMINAL 11 659.0 8 667.4 1 1 664.3 11 665.1 1 1 666.5 t 1 699.0 1 1 665.6 1 1 665.5 1 1 661.9 1 1 655. B 1 1 666.6 11 659. 7 11 661.2 1 1 652.7 SL 030859 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Study ID: K-D01716-091 Volume 2 of 3, Page: 23 Appendix B, continued: Chamber Analyses SL 030860 ANALYTICAL AND NOMINAL CONCENTRATION - MIDDLE DOSE (CONT.l DATE 27-Jul-90 30-Jul-90 ai-jui-90 1-Aug-90 2-Aug-90 3-Aug-9Q 6-Aug-90 7-Aug-90 8-Aug-90 9-Aug-90 10-Aug-90 13-Aug-90 14-Aug-90 15-Aug-90 EXPOSURE _________________________ BEGAN TIME CONC. TIME CONC. 8:41 9:34 633.7 10:04 637.4 12:35 623.2 13:05 617.9 8:50 9:42 624.0 10:12 622.6 8:25 12:43 614.7 13:13 610.9 9:18 N.D. 9:48 N.D. 12:19 618.8 12:49 615.1 8:25 9:18 635.0 9:48 634.8 8:29 12:19 616.6 12:49 N.D. 9:22 652.5 9:52 650.6 12:22 626.1} 12:53 624.6 8:26 9:18 633,1 9:48 632.5 12:19 611.2 12:49 613.8 7:28 8:21 633.5 8:51 633.6 11:22 619.3 11:52 617.9 7:40 8:33 633.5 9:03 638.0 11:34 621.1 12:04 618.1 8:04 8:56 659.5 9:27 661.7 11:57 627.9 12:27 621.2 7:35 8:28 635.8 8:58 648.3 11:29 627.7 11:59 623.1 7:56 8:48 N.D. 9:19 633.0 11:49 623.2 12:19 613.4 7:59 8:51 626.4 9:21 628.6 11:52 610.9 12:22 609.5 7:46 8:21 8:39 11:40 9:13 626.1 613.3 627.2 9:09 12:10 9:43 631.9 606.8 621.3 12:14 606.9 12:44 602.0 TIME 10:34 13:35 10:42 13:43 10:18 13:19 10:18 13:19 10:22 13:23 10:19 13:19 9:21 12:22 9:33 12:34 9:57 12:58 9:28 12:29 9:49 12:49 9:51 12:52 9:39 12:40 10:14 13:14 INDIVIDUAL DATA CONC. TIME 632.8 615.1 624.1 610.0 11:04 14:05 11:13 14:13 627.6 614.0 10:48 13:49 N.D. 614.5 638.8 10:48 13:49 10:52 617.5 13:53 627.8 606.1 10:49 13:50 633.3 9:52 610.0 634.4 12:52 10:03 612.3 655.6 610.6 13:04 10:27 13:28 639.8 9:58 614.4 631.9 605.7 12:59 10:19 13:20 625.4 609.9 10:22 13:22 626.6 600.4 618.1 10:09 13:10 10:44 604.6 13:45 CONC. 627.2 614.1 622.7 606.2 627.8 613.7 628.5 614.8 634.3 615.4 624.8 601.2 620.6 607.6 631.8 611.8 650.0 608.2 637.9 613.7 624.1 605.9 622.3 607.0 626.4 599.3 614.1 603.0 TIME 11:34 14:35 11:43 14:43 11:18 14:19 11:19 14:19 11:22 14:23 11:19 14:20 10:22 13:22 10:34 13:34 10:57 13:58 10:28 13:29 11:19 13:50 10:52 13:53 10:39 13:40 11:14 14:15 CONC. 628.8 611.6 620.9 604.0 629.1 613.1 630.2 N.D. 641.7 613.1 623.6 605.3 626.7 607.3 623.4 607.1 644.8 604.8 632.2 613.7 621.1 603.4 621.6 606.4 616.3 597.7 617.9 599.0 TIME CONC. MEAN 12:05 626.9 624.4 12:13 618.0 616.2 11:49 624.2 620.4 11:49 623.4 624.7 11:52 634.1 631.7 11:49 621.1 618.2 10:52 623.8 621.2 11:04 624.1 623.2 11 :27 641.3 635.0 10:56 637.0 629.4 11:19 619.9 618.2 11:22 617.0 616.6 11:10 619.7 614.9 11:44 608.6 611.2 S.D. 8.7 7.4 6.8 8.6 13.6 11.3 10.0 10.3 21.4 11.8 10.7 8.4 12.4 9.1 N NOMINAL 11 664.1 11 650.3 9 656.0 8 65 3.8 11 650. 7 11 651.1 11 654.9 11 654.7 11 658 . 6 11 650.9 10 652.5 11 653.0 1 1 646.3 11 646.4 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Study ID: K-001716-1)91 Volume 2 of 3, Page: 24 Appendix B, continued: Chamber Analyses ANALYTICAL AND NOMINAL CONCENTRATION - MIDDLE DOSE (CONT.> DATE 16-Aug-90 17-Aug-90 20-Aug-90 2l-Aug-90 22-Aug-90 23-Aug-90 24-Aug-90 27-Aug-90 28-Aug-90 29-Aug-90 30-Aug-90 31-Aug-90 EXPOSURE BEGAN TIME 7:36 8:28 11:29 7:34 8:26 11:27 8:22 9:15 12:16 8:00 8:52 11:53 8:30 9:23 12:24 8:15 9:07 12:08 8:14 9:06 12:07 8:24 9:17 12:17 8:38 9:30 12:31 8:23 9:16 12:17 8:23 9:15 12:16 8:19 9:12 12:13 CONC. 624.1 621.1 627.4 617.5 624.8 599.4 627.7 607.1 627.9 604. <} 638.3 633.1 645.1 629.1 64B.9 627.8 657.6 635.6 647.4 629.9 646.7 635.4 640.2 606.5 TIME 8 58 11 59 8 56 11 57 9 45 12 46 9 22 12 23 9 53 12 54 9 37 12 38 9 37 12 37 9 47 12 47 10 00 13 01 9 46 12 47 9 45 12 46 9 42 12 43 CONC. 626.3 606.0 621.0 617.7 634.0 594.8 625.8 602.1 623.6 603.7 631.9 630.5 639.0 625.9 638.5 622.5 655.4 628.6 644.0 616.4 648.6 625.0 634.2 602.4 TIME 9 28 12 29 9 26 12 27 10 15 13 16 9 53 12 53 10 23 13 24 10 07 13 08 10 07 13 07 10 17 13 18 10 30 13 31 10 16 13 17 10 15 13 16 10 12 13 13 INDIVIDUAL DATA CONC. TIME 625.9 9:58 602.5 12:59 623.9 9:56 611.4 12:57 622.1 10:45 592.5 13:46 617.9 10:23 596.5 13:24 617.3 10:53 596.4 13:54 N.D. 10:38 624.0 13:38 641.7 10:37 615.9 13:38 636.5 10:47 622.5 13:48 649.7 11:00 627.1 14:01 645.3 10:46 612.4 651.2 13:47 10:45 619.1 13:46 628.6 10:42 602.0 13:43 CONC. 626.4 599.4 626.6 604.1 618.2 591.1 616.5 601.8 617.9 609.8 670.1 622.1 641.8 615.4 632.2 620.5 648.3 610.1 637.2 609.5 641.2 611.5 619.6 605.4 TIME 10:29 13:29 10:27 13:27 11:16 14:17 10:53 13:54 11:23 14:24 11:08 14:09 11:07 14:08 11:17 14:18 11:31 14:31 11:16 14:17 11:16 14:16 11:12 14:13 CONC. 620.2 598.3 622.1 602.3 612.2 585.4 614.9 592.5 609.9 611 .4 631.1 618.3 630.8 612.5 630.0 612.9 646.7 609.5 639.1 605.3 638.1 612.3 622.1 603.8 TIME CONC. MEAN S.D. 10:59 620.6 615.5 11.5 10:57 626.4 618.2 8.8 11:46 603.6 607.1 16.1 11:23 609.8 610.2 11.5 11:53 609.6 612.0 9.1 11:38 629.4 632.9 14.3 11:37 630.9 629.8 11.5 11:47 N.D. 629.4 10.5 12:01 640.9 637.2 16.8 11:47 629.8 62 a. 7 15.4 11:46 633.1 633.1 14.4 11:42 613.6 616.2 13.6 NMEAN T.W.A. S.D. RANGE 68 620.2 18.38 526.3 640.5 N NOMINAL 11 650.6 11 651 . 3 11 643.4 11 650.6 11 652.0 10 661.4 11 654.5 10 653.2 11 664.9 11 664.3 11 666.5 11 649.0 656.3 14.06 592.1 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Study ID: K-001716-091 Volume 2 of 3, Page: 25 SL 030862 Appendix B, continued: Chamber Analyses ANALYTICAL, AND NOMINAL CONCENTRATION - HIGH DOSE DATE 29-Nay-90 30-May-90 31-May-90 l-Jun-90 4-Jun-90 5-Jun-90 6-Jun-90 7-Jun-90 8-Jun-90 1l-Jun-90 12-Jun-91 13-Jun-90 M-Jun-90 15-Jun-90 EXPOSURE BEGAN TIME 8:43 7:44 9:44 12:45 9:44 12:46 9:05 10:06 13:07 8:43 9:00 9:43 12:45 10:03 13:04 8:57 9:00 8:47 8:43 8:45 8:36 9:57 12:59 9:59 13:01 9:49 12:51 9:44 12:45 9:47 12:53 9:36 12:38 8:46 8:46 8:48 9:46 12:48 9:46 12:48 9:48 12:49 CONC. 1892.8 2095.7 2044.0 2024.7 1972.2 2052.8 1946.4 2035.4 1925.4 1907,9 1993.7 2228.5 2064.0 2039.0 2016.2 2024.0 2007.1 2022.2 2046.6 2061.9 2047.5 2058.6 2066.7 2081.5 2013.1 2020.4 2050.3 2055.4 TIME 10:14 13:16 10:15 13:16 10:36 13:37 10:13 13:15 10:33 13:35 10:27 13:29 10:29 13:31 10:20 13:21 10:14 13:15 10:18 13:24 10:06 13:08 10:17 13:18 10:17 13:18 10:18 13:20 CONC. 2111.1 2093.0 2055.5 2023.4 2020.6 2052.1 2012.1 2031.8 1938.7 1887.8 2447.4 2090.1 2113.3 2044.6 2024.2 2030.8 2014.5 2020.5 2050.4 2068.5 2060.8 2059.7 2064.5 2089.5 2010.2 2027.2 2049.1 2063.5 TIME 10 44 13 46 10 45 13 47 11 06 14 08 10 44 13 45 11 03 14 05 10 58 13 59 10 59 14 01 10 50 13 52 10 44 13 46 10 49 13 55 10 37 13 38 10 47 13 48 10 47 13 48 10 48 13 50 INDIVIDUAL DATA CONC. TIME 2076.1 11:14 2090.5 13:46 2034.3 11:15 2009.2 2037.5 2050.9 .13:47 11 :37 14:08 2019.4 2032.6 1931.5 11:14 13:45 11:33 1996.1 14:05 2303.7 2102.8 2069.3 2025.5 2015.5 11:28 14:29 11:30 14:31 11:20 2023.2 2013.9 2028.2 14:22 11:14 14:16 2062.6 11:20 2052.0 2055.4 2057.1 N.D. 14:26 11:07 14:09 11:17 2081.4 2010.3 2027.5 14:19 11:17 14:19 2059.5 11:18 2059.7 14:20 CONC. 2087.4 2090.5 2025.0 2009.2 2105.1 2050.9 2040.4 2032.6 1924.9 1996.1 2305.0 2106.0 2044.3 2031.9 2033.7 2023.2 2014.3 2030.1 2073.8 2065.3 2063.4 2066.5 N.D. 2039.3 2021.2 2009.0 2060.6 2060.2 TIME CONC. 11:45 2090.1 11:45 2021.7 12:07 2050.4 11:44 2046.4 12:04 1918.1 11:58 2244.7 12:00 2043.8 11:51 1923.9 11:45 2021.0 11:51 2064.6 11:37 2073.1 11:47 2076.1 11:47 2023.3 11:49 2054.6 TIME CONC. MEAN 12:15 2091.1 2071.8 12:16 2019.7 2026.6 12:37 2051.2 2044.4 12:14 2041.2 2023.8 12:34 1914.3 1934.1 12:28 2233.5 2205.5 12:30 2039.6 2051.5 12:21 2030.5 2014.5 12:15 2023.2 2019.5 12:22 2062.9 2060.9 12:08 2074.0 1744.6 12:18 2077.8 2072.1 12:18 2022.4 2018.5 12:19 2056.3 2056.9 S.D. 63.5 14.5 33.0 29.0 35.5 133.1 25,4 32.4 1. 1 8.5 7 14 . 1 15.5 7.2 4.6 N NOMINAL 10 2622.5 10 2591.5 10 2571.4 10 2554.1 10 2488.0 10 2408.0 10 1990.0 10 2191.6 10 21/1.9 10 2202.0 1 3 2180.2 8 21/2.9 10 2204.7 10 2188.1 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Study ID: K-001716-091 Volume 2 of 3, Page: 26 Appendix B, continued: Chamber Analyses ANALYTICAL AND NOMINAL CONCENTRATION - HIGH DOSE (CONT.t DATE 18-Jun-90 19-Jun-90 20-Jun-90 21-Jun-90 22-Jun-90 25-Jun-90 26-Jan-90 27-Jun-90 29-Jun-90 29-Jun-90 2-JuI-90 3-Jul-90 S-Jul-90 6-Jul-90 INDIVIDUAL EXPOSURE ________________________________________________ DATA BEGAN TIME CONC. TIME CONC. TIME CONC. TIME 8 i 43 9 43 2055.5 10:13 2057.2 10:44 2024.3 11:14 8:37 12 45 2025.5 13:15 2017.0 13:45 2016.6 14:16 9 37 2041.8 10:08 2044.3 10:38 2054.9 11:08 12 39 2053.1 13:09 2052.1 13:40 2058.0 14 :10 8:36 9 36 2034.2 10:06 2038.9 10:37 2045.7 11:07 12 38 1999.4 13:08 2001.5 13:38 1998.0 14:08 8:37 9 38 2026.3 10:08 2028.2 10:38 2036.3 11:08 12 39 2033.3 13:09 2029.7 13:40 2024.5 14:10 8:38 9 39 2002.5 10:09 2006.0 10:39 2014.5 11:09 12 40 2025,6 13:11 2022.8 13:41 2012.7 14:11 8:48 9 48 1968.3 10:19 1970.4 10:49 1963.8 11:19 12 50 1947.5 13:20 1948.5 13:50 1949.8 14:21 8:23 9 23 2013.8 9:53 2013.8 10:23 2003.8 10:54 8:30 12 25 2022.9 12:55 2023.0 13:25 2018.2 13:55 9 35 2041.8 10:05 2046.1 10:36 2050.5 11:06 12 37 2055.0 13:07 2060.4 13:37 2062.6 14:08 8:38 9 39 2030.8 10:09 2033.7 10:39 2034.0 11:09 12 40 2039.9 13:11 2053.7 13:41 2063.9 14:11 8:30 9 30 2050.0 10:00 2051.4 10:30 2062.2 11:01 12 31 N.D. 13:02 N.D. 13:32 2066.5 14:02 8:32 9 32 2068.2 10:02 2059.3 10:33 2069.7 11:03 12 34 2067.1 13:04 2072.5 13:34 2061.6 14:04 8:48 9 49 2066.6 10:19 2055.4 10:49 2071.7 11:19 12 50 2078.6 13:21 N.D. 13:51 N.D. 14:21 8:40 9 39 2014.2 10:10 2017.9 10:40 2021.8 11:10 8:34 12 41 2024.2 13:11 2024.6 13:41 2029.3 14:12 9 34 1977.4 10:04 1986.0 10:34 1986.6 11:05 12 36 1993.2 13:06 1990.0 13:36 1983.9 14:06 CONC. 2023.3 2023.3 2063.2 2063.9 2053.2 1995.5 2041.1 2031.3 2015.1 2020.3 1958.2 1944.1 2018.6 2027.6 2046.5 2057.5 2044.3 2054.5 2058.0 2050.2 2072.7 2062.8 2075.7 N.D. 2023.1 2022.9 1990.6 1989.2 TIME CONC. 11:44 2019.1 11:39 2057.1 11:37 2013.6 11:38 2030.6 11:40 2014.9 11:49 1956.2 11:24 2025.0 11:36 2063.6 11:40 2052.5 11:31 2070.4 11:33 2072.7 11:50 N.D. 11:40 2017.4 11:35 1992.6 TIME CONC. MEAN 12:15 2032.7 2029.4 12:09 2061.4 2055.0 12:07 2002.6 2018.3 12:09 2038.9 2032.0 12:10 2022.6 2015.7 12:20 1957.0 1956.4 11:54 2023.7 2019.0 12:06 N.D. 2053.8 12:10 2049.3 2045.7 12:01 N.D. 2058.4 12:03 N.D. 2067.4 12:20 2082.1 2071.7 12:11 2021.9 2021.7 12:05 1993.9 1988.3 S.D. 14.9 7.5 22.3 5.4 7.4 9.0 7.1 7.9 10.9 8.3 5.1 9.6 4.3 5.0 N NOMINAL 10 2174.9 10 2187.2 10 2221.6 10 2170.8 10 2155.7 10 2003.3 10 2174.0 9 2162.9 10 2158.8 7 2173.6 9 2170.4 6 2140.3 10 21/0.2 10 2177.1 SL 030863 SL 030864 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Study ID: K-001716-091 Volume 2 of 3, Page: 27 Appendix B, continued: Chamber Analyses ANALYTICAL ANO NOMINAL CONCENTRATION - HIGH DOSE ICONT.1 DATE 9-Jul-90 10-Jul-90 1 l-Jui-90 12-Jul-90 13-Jul-90 16-Jul-90 n-jui-9o i8-jui-9o 19-Jul-90 20-Jul-90 2 3-Jul-90 24-Jul-90 25-Jul-90 2 6-Jul- 90 EXPOSURE BEGAN TIME 8:33 9:33 12:35 8:52 9:50 12:51 8:30 9:30 12:32 8:45 9:45 12:45 8:37 9:36 12:37 8:52 9:52 12:53 8:58 9:58 12:59 8:20 9:20 12:21 8:45 9:45 12:46 8:42 9:42 12:42 8:36 9:36 12:37 8:48 9:48 12:49 8:16 9:16 12:17 8:44 9:44 12:45 CONC. 1995.3 2005.4 1992.2 2004.0 1991.9 2004.7 1975.3 1990.4 2010.4 2020,6 1999.2 2025.0 1988.6 2005.2 1998.9 2003.4 1989.6 2001.0 1966.4 1974.0 1964.5 1988.7 1978.9 1999.4 1976.5 1976.9 1968.4 1980.3 TIME 10:04 13:05 10:20 13:22 10:00 13:02 10:15 13:16 10:06 13:07 10:22 13:23 10:28 13:29 9:50 12:51 10:15 13:16 10:12 13:13 10:06 13:07 10:18 13:19 9:46 12:47 10:14 13:15 CONC. 1997.4 2005.7 1998.9 N.0. 1996.0 2003.3 1984.0 1995.0 2017.6 2017.5 2007.3 2026.2 1990.7 2003.2 1994.6 2002.9 1994.2 2002.3 1965.5 1979.5 1966.5 1997.7 1995.0 1999.3 1982.7 1977.1 1969.6 1975.4 TIME 10:34 13:36 10:51 13:52 10:30 13:32 10:45 13:4$ 10:36 13:37 10:53 13:53 10:58 13:59 10:21 13:21 10:45 13:46 10:42 13:43 10:36 13:37 10:49 13:49 10:17 13:18 10:44 13:45 INDIVIDUAL DATA CONC. TIME 2007.5 11:04 2003.3 14:06 2002.9 11:21 N.D. ,14:22 2009.6 11:01 2003.6 14:02 1987.5 11:15 1988.7 14:16 2014.8 11:06 2014.1 14:07 2001.5 11:23 2030.1 14:23 1994.7 11:29 2002.9 14:29 1991.0 10:51 2003.7 13:52 1996.5 11:15 2006.9 14:16 1976.6 11:12 1976.3 14:13 1967.6 11:06 1991.2 14:07 1993.7 11:19 1993.6 14:20 1977.3 10:47 1979.4 13:48 1980.3 11:15 1977.0 14:15 CONC. 2014.7 2004.5 2005.5 N.0. 2007.3 2006.1 1986.8 1988.7 2016.1 2017.6 2003.8 2026.4 2002.3 2011.5 1996.0 1999.6 2000.8 2002.5 1982.4 1977.6 1969.5 1997.8 1999.1 1996.8 1976.6 1968.4 1978.4 1982.2 TIME CONC, 11:35 2015.1 11:51 2008.1 11:31 2003.1 11:45 1992.2 11:37 2024.7 11:53 2016.5 11:59 2004.1 11:21 2003.4 11:45 2006.0 11:42 1984.6 11:36 1994.9 11:49 1998.9 11:17 1986.5 11:45 1976.9 TIME CONC. 12:05 2012.8 12:21 1998.6 12:01 2010.6 12:15 1990.1 12:07 2022.3 12:23 2016.2 12:29 2002.7 11:51 1999.9 12:15 2008.2 12:12 1976.0 12:07 2000.7 12:19 2002.8 11:47 1987.4 12:15 1982.0 MEAN 2006.2 2001.5 2003.6 1987.9 2017.6 2015.2 2000.6 1999.3 2000.8 1975.9 1983.9 1995.8 19/8.9 1977.1 S.D. 6.7 5. 3 5.8 5.3 4.2 11.5 7 .) 4.3 5.9 6.1 15.0 6.6 5.5 4.8 N NOMINAL 10 2171 .6 7 2196.1 10 2156.2 10 2169.0 10 2184.5 10 22/4.3 10 2161.2 10 2180.2 10 2166.8 10 2156.9 10 21/6.1 10 2183.2 10 2185.9 10 2165.0 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Study ID: K-001716-091 Volume 2 of 3, Page: 28 Appendix B, continued: Chamber Analyses ANALYTICAL AND NOMINAL CONCENTRATION - HIGH DOSE (CONT.) DATE 27-Jul-90 30-Jul-90 31-Jul-90 1-Aug-90 2-Aug-90 3-Aug-90 6-Aug-90 1-Aug-90 8-Aug-90 9-Aug-90 lQ-Aug-90 13-Aug-90 14-Aug-90 15-Aug-90 EXPOSURE BEGAN TIME 8:41 8:50 9:41 12:42 9:50 12:51 8:25 8:25 8:29 8:26 9:25 12:26 9:25 12:26 9:29 12:30 9:26 12:27 7:28 8:29 7:40 11:29 8:40 11:41 8:04 7:35 9:04 12:05 8:35 7:56 11:36 8:56 11:57 7:59 7:46 8:21 8:59 11:59 8:46 11:47 9:21 12:22 CONC. 1977.0 1982.8 1975.8 2000.1 N.D. 2005.7 1979.5 1989.6 1984.5 2000.4 1980.3 1996.3 1915.1 1915.3 1987.0 2009.5 2021.1 1999.0 1988.1 1999.3 N.D. 2001.2 1986.3 2012.0 1985.0 2002.6 1996.3 2022.8 TIME 10:12 13:12 10:20 13:21 9:56 12:56 9:56 12:56 9:59 13:00 9:56 12:57 8:59 12:00 9:11 12:11 9:34 12:35 9:05 12:06 9:26 12:27 9:29 12:30 9:17 12:17 9:51 12:52 CONC. TIME 1968.2 10:42 1983.8 13:42 1987.0 . 10:50 1990.2 13:51 2029.2 10:26 2008.1 13:26 1987.9 10:26 N.D. 13:27 2015.8 10:29 1997.6 13:30 1980.4 10:26 2019.0 13:27 1919.6 9:29 1999.8 12:30 1997.3 9:41 2001.0 12:42 2031.0 10:04 1997.9 13:05 2013.3 9:36 2010.8 12:36 1976.1 9:56 1989.0 12:57 1997.2 9:59 2008.1 13:00 2002.6 9:47 2002.1 12:47 1992.3 10:21 2008.3 13:22 INDIVIDUAL DATA CONC. TIME 1970.4 11:12 1980.5 14:13 1991.1 11:20 1987.5 .14:21 2012.0 10:56 2010.0 13:57 N.D. 10:56 1993.9 13:57 1995.0 11:00 2003.8 14:00 1985.3 10:56 2021.4 13:57 1910.4 9:59 1991.6 13:00 2005.4 10:11 2013.0 13:12 2043.4 10:34 1985.3 13:35 2006.4 10:06 1987.9 13:06 1981.8 10:26 1978.3 13:27 1998.9 10:29 2009.3 13:30 1989.9 10:17 2003.1 13:18 2005.8 10:51 2017.3 13:52 CONC. 1983.8 1979.4 1995.6 1992.4 2014.5 2001.1 1998.5 1978.8 2006.1 2000.8 1985.3 2012.1 1915.4 1991.2 2008.8 2004.5 2035.2 1987.8 2011.9 2002.2 1980.6 1984.9 1995.6 2006.4 1991.0 2003.4 2003.3 2017.5 TIME 11:42 11:50 11:26 11:26 11:30 11:26 10:29 10:41 11:05 10:36 10:56 10:59 10:47 11:21 CONC. 1989.7 2004.3 2025.5 1994.6 2012.8 2021.9 1907.3 1994.4 2027.3 2019.7 1981.8 1998.8 2004.3 2007.5 TIME CONC. MEAN 12:12 1983.4 1979.9 12:20 1999.4 1992.3 11:56 2014.2 2013.4 11:56 1992.2 1989.4 12:00 2023.8 2004.1 11: 57 2021.2 2002.3 10:59 1908.5 1937.4 11:11 2006.5 2002.7 11:35 2030.6 2015.9 11:06 2006.6 2004.6 11:27 1986.4 1984.4 11:29 1994.4 2000.7 11:17 2013.1 1999.7 11:51 2023.5 2009.5 S.D. 6.5 8.1 9.0 7.1 11.2 18.4 39.4 t. 9 21.3 10.5 7.4 8.0 8.4 10.7 N NOMINAL 10 2199.6 10 2173.6 9 2191.5 8 2172.5 10 2155.9 10 2188.3 10 2094.8 10 21)9.5 10 2173.0 10 2146.3 9 2152.1 10 2180.9 10 2169.1 10 2185.9 030865 SL 030866 1,1,IT subchronic neurotoxicity The Dow Chemical Company Study ID: K-001716-091 Volume 2 of 3, Page: 29 Appendix B, continued: Chamber Analyses ANALYTICAL AND NOMINAL CONCENTRATION - HIGH DOSE (CONT.) DATE 16-Aug-90 17-Aug-9G 20-Aug-90 21-Aug-90 22-Aug-90 23-Aug-90 24-Aug-90 27-Aug-90 28-Aug-90 29-Aug-90 30-Aug-90 31-Aug-90 INDIVIDUAL EXPOSURE___________________________________DATA BEGAN TIME CONC. TIME CONC. TIME CONC. TIME 7:36 7:34 8:36 11:36 8:34 1998.7 2003.5 1998.5 9:06 12:06 9:04 2002.6 2009.2 1999.0 9 36 12 37 9 34 2006.2 2016.6 1995.6 10:06 13:07 10:04 8:22 8:00 8:30 11:34 9:23 12:23 9:00 12:01 9:30 12:31 2013.9 1975.9 2008.7 1995.7 2019.5 2009.6 2037,3 12:05 9:53 12:54 9:30 12:31 10:00 13:01 2004.3 1996.7 1999.7 1994.3 2007.7 2014.0 2034.3 12 35 10 23 13 24 10 00 13 01 10 31 13 31 1998.6 1992.8 1997.7 1996.2 2016.5 2035.4 2020.1 13:05 10:53 13:54 10:30 13:31 11:01 14:02 8:15 8:14 8:24 9:15 12:15 9:14 12:15 9:24 2027.3 2108.7 2044.7 2092.8 2052.9 9:45 12:46 9:44 12:45 9:54 2031 .0 2122.8 2080.4 2091.3 2068.0 10 15 13 16 10 14 13 15 10 24 N.D. 2106.2 2084.5 2076.9 2088.4 10:45 13:46 10:44 13:45 10:54 8:38 12:25 9:38 12:39 2085.1 2057.6 2072.3 12:55 10:08 13:09 2075.9 2066.4 2067.4 13 25 10 38 13 39 2081.5 2054.3 2055.9 13:55 11:08 14:09 8:23 9:23 2005.5 9:53 2002.6 10 24 2026.3 10:54 8:23 12:24 2029.2 12:54 2024.9 13 25 2024.4 13:55 9:22 2019.9 9:53 2029.2 10 23 2032.3 10:53 8:19 12:23 9:19 12:20 2042.2 2019.1 2031.6 12:54 9:49 12:50 2040.3 2020.6 2026.9 13 24 10 19 13 20 2039.6 2035.2 2034.8 13:54 10:50 13:50 CONC. 2014.8 2013.1 2006.4 2004.3 2004.5 1986.4 2014.0 2017.8 2030.4 2029.0 2150.6 2109.2 2101.3 2070.3 2071.6 2080.8 2062.2 2057.4 2022.9 2022.2 2025.7 2024.3 2027.2 2040.3 TIME CONC. 10:36 2014.4 10:34 2007.1 11:23 2003.1 11:00 2025.0 11:31 2032.2 11:15 2105.0 11:15 2105.3 11:25 2064.9 11:38 2073.8 11:24 2031.0 11:23 2018.1 11:20 2031.2 TIME CONC. 11:06 2009.0 11:04 2004.0 11:53 2008.0 11:30 2013.4 12:01 2038.0 11:45 2119.7 11:45 2093.2 11:55 N.D. 12:08 2078.9 11:54 2029.6 11:53 2032.5 11:50 2047.0 N*= MEAN T.W.A. S.D. RANGE MEAN 2008.8 2003.2 1997.3 2010.0 2028.0 2097.8 2084.0 2074.3 2064.6 2021.9 2030.4 2031.4 60 2016.3 52.09 1745 S.D. 6.0 5.4 10.2 11.0 10.0 41.4 17.5 11.2 8,5 9.8 8.5 8.5 2206 N NOMINAL 10 2197.2 10 21(8.5 10 2174.9 10 2204.2 10 2196.1 9 2216.6 10 2208.7 9 2194.2 10 21B9.1 10 2189.1 10 2)93.9 10 2183.6 68 2207.2 111.8 1990 1,1,1-T subchronic neurotoxicity Appendix C, Clinical Observations The Dow Chemical Company Study ID: K-001716-091 Volume 2 of 3, Page: 30 CONC. PPM ANIMAL NUMBER 90A3792 90A3793 90A3794 90A3795 90A3796 90A3797 90A379B 90A3799 90A3800 90A3801 90A3802 90A3803 90A3804 90A3805 TEST DAY 1-133 1-100 64 1-87 88 89-133 1-100 1-133 ' 1-66 67 68-100 1-133 1-133 1-92 1-133 1-133 1-100 1-100 INLIFE OBSERVATIONS - HALE EXAM TYPEI OBSERVATION/COMMENT NO REMARKABLE OBSERVATIONS. NO REMARKABLE OBSERVATIONS. SPONTANEOUS NO REMARKABLE OBSERVATIONS. CL COMMENT: SHELLING AROUND RIGHT EYE NO REMARKABLE OBSERVATIONS. NO REMARKABLE OBSERVATIONS. NO REMARKABLE OBSERVATIONS. NO REMARKABLE OBSERVATIONS. CL COMMENT: BLEEDING SLIGHTLY AT SITE OF SURGERY NO REMARKABLE OBSERVATIONS. NO REMARKABLE OBSERVATIONS. NO REMARKABLE OBSERVATIONS. NO REMARKABLE OBSERVATIONS. NO REMARKABLE OBSERVATIONS. NO REMARKABLE OBSERVATIONS. NO REMARKABLE OBSERVATIONS. NO REMARKABLE OBSERVATIONS. t EXAM TYPES: CG - CAGESIDE, CL = CLINICAL, CG* = CAGESIDE, NOTED ON CLINICAL EXAM, CL* = CLINICAL, NOTED ON CAGESIDE EXAM. 030867 *! SL 030868 1,1,1-T subchronic neurotoxicity Appendix C, continued: Clinical Observations The Dow Chemical Company Study ID: K-001716-091 Volume 2 of 3, Page: 31 CONC. PPH 200 ANIMAL NUMBER 90A3806 90A3807 90A380B 90A3809 90A3810 90A38I1 90A38I2 90A3B13 90A38H 90A3B1S 90A3816 90A3817 90A3B18 90A3819 TEST DAY 1-133 1-100 1-133 1-133 123 1-100 67 , 1-66' 68-133 1-100 1-100 1-133 1-93 1-133 1-133 67 1-66 68-100 INLIFE OBSERVATIONS - HALE EXAM TYPE! OBSERVATION/COMMENT NO REMARKABLE OBSERVATIONS. NO REMARKABLE OBSERVATIONS. NO REMARKABLE OBSERVATIONS. NO REMARKABLE OBSERVATIONS. SPONTANEOUS DEATH NO REMARKABLE OBSERVATIONS. CL COMMENT: BLEEDING SLIGHTLY AT SITE OF SURGERY NO REMARKABLE OBSERVATIONS. NO REMARKABLE OBSERVATIONS. NO REMARKABLE OBSERVATIONS. NO REMARKABLE OBSERVATIONS. NO REMARKABLE OBSERVATIONS. NO REMARKABLE OBSERVATIONS. NO REMARKABLE OBSERVATIONS. NO REMARKABLE OBSERVATIONS. CL COMMENT: BLEEDING SLIGHTLY AT SITE OF SURGERY NO REMARKABLE OBSERVATIONS. NO REMARKABLE OBSERVATIONS. I EXAM TYPES: CG - CAGES IDE, CL - CLINICAL, CG CAGESIDE, NOTED ON CLINICAL EXAM, CL CLINICAL, NOTED ON CAGESIDE EXAM. 1,1,1-T subchronic neurotoxicity TABLE Appendix C, continued: Clinical Observations The Dow Chemical Company Study ID: K-001716-091 Volume 2 of 3, Page: 32 CONC. PPM 630 ANIMAL NUMBER 90A3820 90A3821 90A3S22 9OA3023 90A3824 9OA3025 90A3S26 90A382T 90A382B 90A3829 90A3830 90A3831 90A3832 90A3833 TEST DAY 08-133 1-87 74 1-73 75-100 126 1-133 1-133 1-100. 67 1-66 68-100 63 1-133 1-133 1-100 1-127 1-133 88-100 1-87 INLIFE OBSERVATIONS - MALE EXAM TYPEI OBSERVATION/COMMENT CL EXCESSIVE CHROMODACYORRHEA, RIGHT NO REMARKABLE OBSERVATIONS. CL COMMENT: BLACK CRUST AROUND RIGHT EYE NO REMARKABLE OBSERVATIONS. NO REMARKABLE OBSERVATIONS. MORIBUND NO REMARKABLE OBSERVATIONS. NO REMARKABLE OBSERVATIONS. NO REMARKABLE OBSERVATIONS. CL COMMENT: BLEEDING SLIGHTLY AT SITE OF SURGERY NO REHARKABLE OBSERVATIONS. NO REMARKABLE OBSERVATIONS. SPONTANEOUS NO REMARKABLE OBSERVATIONS. NO REMARKABLE OBSERVATIONS. NO REMARKABLE OBSERVATIONS. NO REMARKABLE OBSERVATIONS. NO REMARKABLE OBSERVATIONS. CL EXCESSIVE CHROMODACYORRHEA, RIGHT NO REMARKABLE OBSERVATIONS. t EXAM TYPES: CG - CAGESIDE, CL - CLINICAL, CG* - CAGESIDE, NOTED ON CLINICAL EXAM, CL* = CLINICAL, NOTED ON CAGESIDE EXAM. 030869 1,1,1-T subchronic neurotoxicity Appendix C, continued: Clinical Observations The Dow Chemical Company Study ID: K-001716-091 Volume 2 of 3, Page: 33 030870 CONC. PPM 2000 ANIMAL NUMBER 90A3834 90A3835 90A3836 90A3837 90A3838 90A3639 90A3840 90A3841 90A3812 90A3843 90A3844 90A3845 90A3846 90A3847 TEST DAT 11 19-46 19 25 74-81 1-10 1-18 19-53 54-133 1-92 1-100 1-100 46-53 1-45 54-121 67 1-66 1-133 1-133 1-133 1-133 1-100 1-100 58 58 1-57 1-133 INLIFE OBSERVATIONS - MALE EXAM TTPEI OBSERVATION/COMMENT CL COMMENT: SHELLING AROUND RIGHT ETE CL PERINEAL SOILING CL COMMENT: SHELLING IS NO LONGER FOUND CL COMMENT; PSL NO LONGER FOUND CL PERINEAL SOILING NO REMARKABLE OBSERVATIONS. NO REMARKABLE OBSERVATIONS. CL PERINEAL SOILING NO REMARKABLE OBSERVATIONS. NO REMARKABLE OBSERVATIONS. NO REMARKABLE OBSERVATIONS. NO REMARKABLE OBSERVATIONS. CL PERINEAL SOILING NO REMARKABLE OBSERVATIONS. NO REMARKABLE OBSERVATIONS. CL COMMENT: BLEEDING SLIGHTLT AT SITE OF SURGERY NO REMARKABLE OBSERVATIONS. NO REMARKABLE OBSERVATIONS. NO REMARKABLE OBSERVATIONS. NO REMARKABLE OBSERVATIONS. NO REMARKABLE OBSERVATIONS. NO REMARKABLE OBSERVATIONS. NO REMARKABLE OBSERVATIONS. CG COMMENT: FOOD AND WATER CHECKED- BOTH AVAILABLE SPONTANEOUS DEATH NO REMARKABLE OBSERVATIONS. NO REMARKABLE OBSERVATIONS. t EXAM TTPES: CG >= CAGES IDE, CL " CLINICAL, CG* * CAGESIDE, NOTED ON CLINICAL EXAM, CL* = CLINICAL, NOTED ON CAGES 1DE EXAM. 1,1,1-T subchronic neurotoxicity Appendix C, continued: Clinical Observations The Dow Chemical Company Study ID: K-001716-091 Volume 2 of 3, Page: 34 CONC. PPM 0 ANIMAL NUMBER 90A3848 90A3849 90A38S0 90A3B51 90A38S2 90A3853 90A3854 90A385S 90A3856 90A3857 90A3858 90A3859 90A3860 90A3861 TEST DAY 1-133 1-133 81-88 1-80 89-133 1-133 1-100 1-133, 88-100 1-87 1-100 1-100 1-100 1-93 1-120 1-133 119-133 1-118 INLIEE OBSERVATIONS - FEMALE EXAM TYPE! OBSERVATION/COMMENT NO REMARKABLE OBSERVATIONS. NO REMARKABLE OBSERVATIONS. CL EXCESSIVE CHROMODACYORRHEA, RIGHT NO REMARKABLE OBSERVATIONS. NO REMARKABLE OBSERVATIONS. NO REMARKABLE OBSERVATIONS. NO REMARKABLE OBSERVATIONS. NO REMARKABLE OBSERVATIONS. CL EYELIDS CLOSED, LEFT NO REMARKABLE OBSERVATIONS. NO REMARKABLE OBSERVATIONS. NO REMARKABLE OBSERVATIONS. NO REMARKABLE OBSERVATIONS. NO REMARKABLE OBSERVATIONS. NO REMARKABLE OBSERVATIONS. NO REMARKABLE OBSERVATIONS. CG EYE OPAQUE, LEFT NO REMARKABLE OBSERVATIONS. X Z .8 0 0 I EXAM TYPES: CG - CAGES IDE, CL - CLINICAL, CG* = CAGESIDE, NOTED ON CLINICAL EXAM, CL* - CLINICAL, NOTED ON CAGESIDE EXAM. n 030872 1,1,1 -T subchronic neurotoxicity Appendix C, continued: Clinical Observations The Dow Chemical Company Study ID: K-001716-091 Volume 2 of 3, Page: 35 CONC. PPM 200 ANIMAL NUMBER 90A3862 90A3863 90A3B64 90A3865 90A3866 90A3867 90A3868 90A3B69 90A3870 90A3871 90A3872 90A3B73 90A3874 90A3875 TEST DAY 1-100 1-133 74 1-73 1-100 1-100 66 1-133 1-133 . 1-100 1-133 1-92 1-133 1-133 1-133 39 52 86-100 1-38 40-51 53-87 INLIFE OBSERVATIONS - FEMALE EXAM TYPEI OBSERVATION/COMMENT NO REMARKABLE OBSERVATIONS. NO REMARKABLE OBSERVATIONS. CL COMMENT: RIGHT EYE SWOLLEN - SLIGHTLY CLOSED NO REMARKABLE OBSERVATIONS. NO REMARKABLE OBSERVATIONS. NO REMARKABLE OBSERVATIONS. SPONTANEOUS NO REMARKABLE OBSERVATIONS. NO REMARKABLE OBSERVATIONS. NO REMARKABLE OBSERVATIONS. NO REMARKABLE OBSERVATIONS. NO REMARKABLE OBSERVATIONS. NO REMARKABLE OBSERVATIONS. NO REMARKABLE OBSERVATIONS. NO REMARKABLE OBSERVATIONS. CL COMMENT: BLACK CRUST AROUND LEFT EYE CL COMMENT: REDDISH CRUST AND SWELLING AROUND LEFT EYE CL EYELIDS CLOSED, RIGHT NO REMARKABLE OBSERVATIONS. NO REMARKABLE OBSERVATIONS. NO REMARKABLE OBSERVATIONS. I EXAM TYPES: CG - CAGESIDE, CL - CLINICAL, CG* = CAGESIOE, NOTED ON CLINICAL EXAM, CL* = CLINICAL, NOTED ON CAGESIDE EXAM. 1,1,1 -T subchronic neurotoxicity Appendix C, continued: Clinical Observations The Dow Chemical Company Study ID: K-001716-091 Volume 2 of 3, Page: 36 CONC. PPM 630 ANIMAL NUMBER 90A3876 90A3B77 90A3B78 90A3879 90A3880 90A3881 90A3882 90A3883 90A3SS4 90A3885 90A3886 90A3BB7 90A3888 90A3B89 TEST DAV 1-133 1-100 1-133 67-74 1-66 75-133 1-112 1-133 1-100 1-100 ' 1-133 1-100 67 1-66 68-100 1-133 60 1-59 39 46 88 1-38 40-45 47-87 89-133 inlife: observations - female EXAM TVPEI OBSERVATION/COMMENT NO REMARKABLE OBSERVATIONS. NO REMARKABLE OBSERVATIONS. NO REMARKABLE OBSERVATIONS. CL EVELIDS SQUINTED, LEFT NO REMARKABLE OBSERVATIONS. NO REMARKABLE OBSERVATIONS. NO REMARKABLE OBSERVATIONS. NO REMARKABLE OBSERVATIONS. NO REMARKABLE OBSERVATIONS. NO REMARKABLE OBSERVATIONS. NO REMARKABLE OBSERVATIONS. NO REMARKABLE OBSERVATIONS. CL COMMENT: LEFT EVE CLOSED NO REMARKABLE OBSERVATIONS. NO REMARKABLE OBSERVATIONS. NO REMARKABLE OBSERVATIONS. CL COMMENT: SLIGHT SHELLING AROUND LEFT NO REMARKABLE OBSERVATIONS. CL CL CL COMMENT; BLACK CRUST AROUND LEFT EYE COMMENT: SLIGHT SHELLING AROUND LEFT EVE-CRUST COMMENT: BLACK CRUST AROUND RIGHT EVE NO REMARKABLE OBSERVATIONS. NO REMARKABLE OBSERVATIONS. NO REMARKABLE OBSERVATIONS. NO REMARKABLE OBSERVATIONS. NFR SL 030873 I EXAM TVPES: CG - CAGESIDE, CL - CLINICAL, CC* - CAGESIDE, NOTED ON CLINICAL EXAM, CL* = CLINICAL, NOTED ON CAGESIDE EXAM. SL 030874 1,1,1-T subchronic neurotoxicity Appendix C, continued: Clinical Observations The Dow Chemical Company Study ID: K-001716-091 Volume 2 of 3, Page: 37 CONC. PPM 2000 ANIMAL NUMBER 90A3890 90A3891 90A3892 90A3B93 90A3894 90A3895 90A3896 90A3891 90A3898 90A3899 90A3900 90A3901 90A3902 90A3903 TEST DAY 1-133 1-100 14 101 1-100 ee 1-133 61 12 , 52 1-11 13-51 53-100 67 81 1-66 68-00 82-133 1-133 1-133 88 1-81 89-100 1-133 1-133 INLIFE OBSERVATIONS - FEMALE EXAM TYPEI OBSERVATION/COMMENT NO REMARKABLE OBSERVATIONS. NO REMARKABLE OBSERVATIONS. CL COMMENT: BLACK CRUST AROUND LEFT EYE SACRIFICED NO REMARKABLE OBSERVATIONS. SPONTANEOUS NO REMARKABLE OBSERVATIONS. SPONTANEOUS CL COMMENT: REDDISH MATERIAL AROUND LEFT EYE CL COMMENT: REDDISH MATERIAL/SLIGHT SHELLING NO REMARKABLE OBSERVATIONS. NO REMARKABLE OBSERVATIONS. NO REMARKABLE OBSERVATIONS. CL COMMENT: RIGHT EYE CLOSED CL COMMENT: SHELLING AROUND LEFT EYE NO REMARKABLE OBSERVATIONS. NO REMARKABLE OBSERVATIONS. NO REMARKABLE OBSERVATIONS. NO REMARKABLE OBSERVATIONS. NO REMARKABLE OBSERVATIONS. CL COMMENT: BLACK CRUST - LEFT EYE NO REMARKABLE OBSERVATIONS. NO REMARKABLE OBSERVATIONS. NO REMARKABLE OBSERVATIONS. NO REMARKABLE OBSERVATIONS. I EXAM TYPES: CG - CAGESIDE, CL - CLINICAL, CG* CAGESIDE, NOTED ON CLINICAL EXAM, CL CLINICAL, NOTED ON CAGESIDE EXAM. 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 2 of 3, Page: 38 Appendix D: Body Weights m Sex 90A3792 90A3793 90A3794 90A3795 90A3796 90A3797 90A3798 90A3799 90A3800 90A3801 90A3802 90A3803 90A3804 90A3805 Male Male Male Male Male Male Male Male Male Male Male Male Male Male Cone Group. -Jl A 9 Test Dev 21 m 3Z M 51 Oppm Oppm Oppm Oppm Oppm Oppm Oppm Oppm Oppm 0 ppm Oppm Oppm Oppm Oppm A A A A A A A B B B B B B B 292.5 276.5 307.1 290.2 284.4 299.7 297.3 320.8 309.3 281.2 284.6 293.0 298.1 316.0 294.4 289.2 311.6 294.4 288.6 301.7 307.9 329.6 291.9 288.0 287.1 293.8 300.4 311.5 297.3 297.7 319.8 296.6 279.1 301.6 324.2 329.8 319.4 293.1 291.8 302.2 312.8 314.8 301.8 302.9 3323 307.0 2803 305.9 3303 3363 3213 298.4 296.0 3063 3213 309.6 309.0 311.1 339.0 316.4 287.3 3113 337.9 340.7 329.9 302.1 299.5 318.7 328.7 3123 308.4 3103 341.9 316.0 287.2 311.0 3363 347.1 335.7 3013 302.7 3203 331.8 316.9 306.9 307.6 346.8 327.0 290.6 315.9 341.4 3443 343.0 300.7 3043 331.9 335.0 314.4 308.9 310.8 310.6 309.6 3513 356.8 3333 336.8 289.0 2903 319.4 323.6 3493 348.7 346.03 53.7 3493 353.8 2933 297.7 308.4 3103 338.7 344.4 349.0 351.7 315.0 314.7 Mean SD N 296.5 299.3 305.7 3103 3173 319.1 322.1 325.9 3283 13.2 12.2 143 15.7 16.0 173 18.6 22.1 23.1 14 14 14 14 14 14 14 14 14 90A3806 90A3807 90A3808 90A3809 90A3810 90A3811 90A3812 90A3813 90A3814 90A3815 90A3816 90A3817 90A3818 90A3819 Male Male Male Male Male Male Male Male Male Male Male Male Male Male 200 ppm 200 ppm 200 ppm 200 ppm 200 ppm 200 ppm 200 ppm 200 ppm 200 ppm 200 ppm 200 ppm 200 ppm 200 ppm 200 ppm A A A A A A A B B B B B B B 292.3 304.1 317.2 327.9 342.4 3483 3573 3673 374.8 284.3 296.3 305.2 3123 316.8 3163 3263 3303 3343 295.4 303.9 309.0 325.9 3323 3333 336.0 342.2 345.9 299.9 301.1 307.7 3153 3223 319.1 324.6 329.4 336.8 293.9 297.3 303.3 3063 307.1 304.7 3013 307.9 314.4 311.9 324.4 335.2 3393 3453 348.1 355.7 359.9 364.7 275.1 286.5 306.3 310.0 3163 317.2 3253 336.1 3503 317.6 317.6 3223 3303 333.0 339.7 3413 348.6 352.8 296.5 294.4 296.1 300.9 306.6 311.6 322.7 322.0 324.0 284.4 290.1 294.1 299.6 303.0 312.1 316.1 3193 3203 313.2 312.3 318.0 328.0 333.4 3403 3483 343.4 355.9 276.4 269.6 277.1 280.0 284.9 281.9 290.9 2923 296.4 299.8 293.7 300.9 309.4 312.1 312-8 3203 3243 330.0 299.3 301.5 310.1 3133 3233 319.7 333.8 3403 329.4 Mean SD N 295.7 299.5 3073 3143 319.9 321.8 328.6 333.1 337.9 12.9 13.5 13.9 15.4 16.7 18.4 18.9 19.8 21.1 14 14 14 14 14 14 14 14 14 SL 030875 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 2 of 3, Page: 39 Appendix D, continued: Body Weights TestCbv 1X2 Sex Cone Group -6 2 9 16 22 m 2Z 44 51 90A3820 90A3821 90A3822 90A3823 90A3824 90A3825 90A3826 90A3827 90A3828 90A3829 90A3830 90A3831 90A3832 90A3833 Male Male Male Male Male Male Male Male Male Male Male Male Male Male 630 ppm 630 ppm 630 ppm 630 ppm 630 ppm 630 ppm 630 ppm 630 ppm 630 ppm 630 ppm 630 ppm 630 ppm 630 ppm 630 ppm A A A A A A A B B B B B B B 299.4 292.3 285.0 308.5 283.6 291.3 297.1 294.9 287.5 313.4 299.7 305.8 274.9 319.2 301.0 299.2 293.4 322.9 294.6 299.2 300.1 301.4 291.9 314.6 293.8 312.7 286.1 316.2 313.1 309.6 305.7 331.6 301.6 303.9 302.8 308.8 300.2 318.7 295.9 315.2 296.1 313.3 316.7 3173 314.1 3393 309.7 299.1 3073 314.7 303.8 324.6 299.2 3153 304.6 324.9 318.7 3243 327.6 347.7 319.0 3143 319.4 321.8 316.0 326.0 300.1 320.5 307.0 329.8 319.3 327.7 333.4 348.6 3223 313.1 312.7 327.9 320.7 323.0 296.0 323.0 313.3 333.8 327.7 333.4 344.9 357.0 336.2 322.0 323.1 3363 330.0 3263 293.8 325.0 322.9 337.7 334.1 337.0 349.9 3653 342.0 326.9 326.7 3433 342.7 329.2 296.9 327.1 328.9 3483 3383 345.1 352.0 3713 3533 330.7 329.0 3513 353.6 336.7 298.0 335.0 3353 3563 Mean SD N 296.6 301.9 3083 313.7 320.9 3223 329.7 335.6 341.9 12.2 10.7 9.7 11.1 11.0 123 14.2 15.8 173 14 14 14 14 14 14 14 14 14 90A3834 90A3835 90A3836 90A3837 90A3838 90A3839 90A3840 90A3841 90A3842 90A3843 90A3844 90A3845 90A3846 90A3847 Male Male Male Male Male Male Male Male Male Male Male Male Male Male 2000 ppm 2000 ppm 2000 ppm 2000 ppm 2000 ppm 2000 ppm 2000 ppm 2000 ppm 2000 ppm 2000 ppm 2000 ppm 2000 ppm 2000 ppm 2000 ppm A A A A A A A B B B B B B B 300.7 302.8 308.1 3163 317.6 3223 327.8 329.7 337.4 309.5 318.9 328.9 338.6 346.4 349.9 3573 362.4 365.4 286.4 300.8 316.6 329.9 339.8 340.9 3473 358.4 3573 283.2 303.6 312.7 323.7 342.0 319.4 359.7 364.4 3683 289.4 288.3 299.4 3013 304.8 304.4 311.8 3173 322.9 295.2 300.8 304.8 306.7 316.6 320.7 319.9 3303 329.8 293.3 301.0 314.6 3173 321.7 3203 322.9 3243 312.4 310.7 311.1 318.4 332.6 336.9 347.2 351.8 356.9 362.9 321.9 322.1 329.3 3343 3393 3443 345.9 3503 346.8 291.1 292.7 292.0 2963 289.4 2953 294.9 293.6 300.9 299.6 303.2 319.4 3313 336.4 3433 348.8 351.4 357.1 282.8 292.0 305.7 316.9 323.2 329.2 3353 3363 337.8 313.8 318.9 325.0 327.6 325.6 329.4 318.6 314.9 326.1 294.3 286.2 296.2 306.6 288.6 304.8 310.6 319.7 333.1 Mean SD N 298.0 303.0 312.2 320.0 323.5 326.6 332.4 3363 339.9 12.0 11.4 11.7 13.3 18.7 17.2 20.0 21.4 20.7 14 14 14 14 14 14 14 14 14 SL 030876 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 2 of 3, Page: 40 Appendix D, continued: Body Weights IS S 90A3848 Female 90A3849 Female 90A3850 Female 90A3851 Female 90A3852 Female 90A3853 Female 90A3854 Female 90A3855 Female 90A3856 Female 90A3857 Female 90A3858 Female 90A3859 Female 90A3860 Female 90A3861 Female Cone Group -6 l 9 T<stDi>y 22 2Q 37 44 51 Oppm Oppm Oppm Oppm Oppm Oppm Oppm Oppm Oppm Oppm Oppm Oppm Oppm Oppm A A A A A A A B B B B B B B 162.8 166.2 169.7 165.7 168.3 170.6 163.2 168.6 171.8 165.9 169.1 173.8 166.7 162.1 173.0 172.8 173.9 171.6 170.7 174.8 163.3 167.1 171.9 164.8 170.7 175.5 170.8 164.1 172.0 1763 174.4 163.4 175.7 178.0 166.6 1663 173.0 166.3 1703 179.5 171.7 169.0 1763 1743 1823 177.6 178.4 177.4 1663 1723 177.7 167.7 174.0 1813 1763 171.7 179.1 1813 1883 177.8 1833 179.9 171.8 1743 178.4 169.9 176.2 187.6 180.9 171.4 177.4 181.6 1853 1883 1883 189.9 1773 178.7 1833 1843 181.0 1843 171.1 1723 1803 1803 180.5 1843 172.1 1743 178.2 178.0 184.0 188.9 182.0 184.9 174.2 173.4 183.1 192.1 193.7 184.7 1863 184.6 178.9 1853 1883 1793 179.9 1903 1923 1733 184.4 1923 1933 187.7 187.9 1853 1803 1893 185.0 179.2 182.7 190.0 191.0 1803 Mean SD N 167.5 170.4 171.6 175.4 178.6 179.8 181.7 185.1 186.4 3.5 4.0 4.9 4.6 5.6 5.1 5.7 5.9 4.7 14 14 14 14 14 14 14 14 14 90A3862 Female 90A3863 Female 90A3864 Female 90A3865 Female 90A3866 Female 90A3867 Female 90A3868 Female 90A3869 Female 90A3870 Female 90A3871 Female 90A3872 Female 90A3873 Female 90A3874 Female 90A3875 Female 200 ppm 200 ppm 200 ppm 200 ppm 200 ppm 200 ppm 200 ppm 200 ppm 200 ppm 200 ppm 200 ppm 200 ppm 200 ppm 200 ppm A A A A A A A B B B B B B B 166.3 169.5 166.0 167.8 170.8 165.2 161.6 165.8 173.4 171.4 168.6 170.2 163.7 166.8 171.9 171.8 169.5 169.1 171.7 166.0 163.5 1693 173.8 172.0 169.0 1713 163.9 170.6 176.1 1773 173.3 1723 173.9 172.2 167.4 175.6 170.1 174.7 167.6 174.0 167.6 172.4 178.0 180.0 177.7 1733 1763 1733 1723 1783 177.4 1773 1703 174.0 171.7 1803 181.6 1823 1813 179.4 1803 1813 173.1 1813 178.7 178.4 1733 1783 173.0 182.4 1803 181.6 180.6 178.9 186.0 181.7 1733 1823 181.4 1813 174.9 1773 174.6 179.7 181.7 187.1 1813 180.4 1873 1843 1733 1813 177.6 183.9 174.1 180.0 180.6 182.4 1813 188.9 1893 181.0 1893 1853 1723 1905 181.4 1833 1773 184.1 177.9 1823 1803 1893 1873 185.4 192.6 185.7 179.1 189.4 186.6 1883 1823 1893 182.1 1913 Mean SD N 167.6 169.5 1723 1753 179.0 179.7 1813 1833 1863 3.2 3.1 3.2 33 3.5 3.4 4.1 53 4.1 14 14 14 14 14 14 14 14 14 030877 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 2 of 3, Page: 41 Appendix D, continued: Body Weights Test Dav ID Sex Cone Group. A 2 9 A 23 3Q 3Z 44 51 90A3876 Female 90A3877 Female 90A3878 Female 90A3879 Female 90A3880 Female 90A3881 Female 90A3882 Female 90A3883 Female 90A3884 Female 90A3885 Female 90A3886 Female 90A3887 Female 90A3888 Female 90A3889 Female 630 ppm 630 ppm 630 ppm 630 ppm 630 ppm 630 ppm 630 ppm 630 ppm 630 ppm 630 ppm 630 ppm 630 ppm 630 ppm 630 ppm A A A A A A A B B B B B B B 168.1 162.9 166.8 170.6 159.9 165.8 169.2 164.1 172.2 164.2 166.9 168.4 170.1 172.5 177.1 1663 168.6 172.6 1593 1723 173.7 164.1 173.2 163.7 167.6 1713 173.6 175.1 181.3 172.7 173.7 176.0 159.2 178.1 176.9 1663 172.7 1663 166.2 172.6 178.4 173.2 183.1 176.6 1753 178.9 1653 182.6 176.7 168.9 1763 1683 168.1 178.9 182.1 182.1 186.6 183.1 175.4 1823 169.0 187.0 184.1 168.4 179.2 172.0 167.6 185.4 186.6 183.9 191.4 184.4 1763 1843 1673 188.6 1793 170.7 1793 168.5 170.7 181.4 184.8 186.0 190.8 185.9 178.8 1883 174.3 190.0 183.7 174.4 182.6 172.0 173.6 183.4 1863 1913 1923 194.4 182.1 1883 172.7 192.0 184.0 174.4 1863 1733 180.7 187.6 189.1 1913 1943 188.0 182.1 190.4 176.7 191.8 185.4 176.7 190.9 174.9 1773 1883 1923 1973 Mean SD N 1673 169.9 172.4 176.0 1793 1793 182.6 184.9 1863 3.6 5.1 6.0 6.0 73 7.7 63 73 7.4 14 14 14 14 14 14 14 14 14 90A3890 Female 90A3891 Female 90A3892 Female 90A3893 Female 90A3894 Female 90A3895 Female 90A3896 Female 9QA3897 Female 90A3898 Female 90A3899 Female 90A3900 Female 90A3901 Female 90A3902 Female 90A3903 Female 2000 ppm 2000 ppm 2000 ppm 2000 ppm 2000 ppm 2000 ppm 2000 ppm 2000 ppm 2000 ppm 2000 ppm 2000 ppm 2000 ppm 2000 ppm 2000 ppm A A A A A A A B B B B B A A 1673 172.1 166.0 1653 165.7 1633 168.0 172.8 166.6 165.0 170.2 169.0 1693 162.8 164.7 172.2 168.7 172.1 168.9 175.2 164.4 168.0 170.6 174.7 170.7 1633 172.0 1703 175.0 1793 177.2 178.1 173.1 185.5 173.7 166.6 173.8 1773 176.6 166.7 176.3 181.1 1763 179.7 176.7 182.4 171.6 194.0 1753 170.9 174.4 180.7 178.7 168.0 179.2 184.9 180.2 185.7 179.7 1893 175.5 196.2 1823 180.6 175.1 1873 179.7 1733 182.1 188.3 182.4 185.7 1803 1923 176.7 196.8 1823 180.4 179.9 1893 185.1 178.4 1813 188.0 1853 1913 187.4 1953 183.8 200.7 182.9 1853 177.6 1933 182.7 181.7 1833 196.9 1853 1923 1823 197.1 183.4 200.9 190.1 185.0 1833 1893 185.4 1843 186.7 194.0 1893 191.1 184.0 198.0 184.6 205.1 191.9 191.0 185.4 188.6 186.1 186.7 185.8 1993 Mean SD N 167.4 169.7 175.8 178.1 182.6 184.2 187.7 1883 1903 3.0 3.7 5.0 6.5 6.3 5.6 6.7 5.7 6.3 14 14 14 14 14 14 14 14 14 SL 030878 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 2 of 3, Page: 42 Appendix D, continued: Body Weights ID 90A3792 90A3793 90A3794 90A3795 90A3796 90A3797 90A3798 90A3799 90A3800 90A3801 90A3802 90A3803 90A3804 90A3805 90A3806 90A3807 90A3808 90A3809 90A3810 90A3811 90A3812 90A3813 90A3814 90A3815 90A3816 90A3817 90A3818 90A3819 Test Day Sex Cone Group 58 & 72 79 86 n 99 128 Male Male Male Male Male Male Male Male Male Male Male Male Male Male Oppm A 306.4 292.6 304.2 315.0 3233 314.6 b 344.6 Oppm A 310.5 303.9 3093 321.6 319.3 326.4 b a Oppm A 355.0 a a a a a a a Oppm A 343.4 354.2 333.8 342.4 339.8 3505 b 349.7 Oppm A 290.9 295.2 284.7 290.9 298.9 2975 b a Oppm A 326.0 33025 323.4 333.4 345.8 345.0 b 3425 Oppm A 348.8 353.7 335.8 3363 345.8 3485 b a Oppm B 360.5 352.1 361.9 3733 381.6 377.7 3635 396.0 Oppm B 352.8 334.5 340.6 335.4 335.2 3365 325.4 353.4 Oppm B 3015 287.7 296.1 301.6 313.7 318.1 a a Oppm B 317.0 3245 312.8 319.1 323.2 3235 314.3 336.9 Oppm B 338.4 3485 331.6 336.1 3335 334.4 3225 374.6 Oppm B 352.6 360.4 3455 364.1 373.5 3655 3405 a Oppm B 317.2 320.7 317.7 325.7 330.4 3275 311.6 a Mean SD N 330.1 327.6 322.9 330.4 335.7 335.7 329.7 3565 22.9 25.9 215 225 22.7 21.7 195 21.1 14 13 13 13 13 13 6 7 Male 200 ppm A 377.6 364.6 373.7 376.0 383.4 3765 b 4115 Male 200 ppm A 339.1 3255 331.9 337.3 340.2 3435 b a Male 200 ppm A' 347.1 334.6 342.4 347.1 3525 3545 b 3675 Male 200 ppm A 336.9 346.7 327.2 339.7 324.1 3445 b 351.1 Male 200 ppm A 311.8 3203 287.9 273.5 273.9 264.4 b a Male 200 ppm A 367.3 370.7 362.1 372.9 381.2 383.9 b a Male 200 ppm A 345.4 353.1 3313 345.1 348.2 3355 b 3885 Male 200 ppm B 355.2 343.6 3423 343.0 344.7 3325 3215 a Male 200 ppm B 330.4 3153 314.8 321.9 327.1 324.0 308.1 a Male 200 ppm B 323.7 309.4 325.9 336.6 3375 339.6 3255 354.9 Male 200 ppm B 352.6 3643 3545 351.0 3635 3705 a a Male 200 ppm B 295.7 305.4 2965 300.6 309.4 3085 304.7 331.6 Male 200 ppm B 330.8 327.7 3175 3235 3335 3305 3215 3475 Male 200 ppm B 324.0 3323 3243 335.6 346.1 3505 331.0 a Mean SD N 338.4 336.7 330.9 336.0 3405 3395 318.8 364.6 21.6 21.1 235 263 28.0 29.9 105 275 14 14 14 14 14 14 6 7 aAnimal died; ^Data not collected. SL 30879 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 2 of 3, Page: 43 Appendix D, continued: Body Weights m 90A3820 90A3821 90A3822 90A3823 90A3824 90A3825 90A3826 90A3827 90A3828 90A3829 90A3830 90A3831 90A3832 90A3833 90A3834 90A3835 90A3836 90A3S37 90A3838 90A3839 90A3840 90A3841 9QA3842 90A3843 90A3844 90A3845 90A3846 90A3847 Is* Pay Sex Cone Gfoud 5a 65 u 79 aa 92 22 123 Male 630 ppm A 336.0 3262 327.8 338.2 335.0 329.7 b 3622 Male 630 ppm A 346.8 325.7 277.4 265.4 2932 309.4 b a Male 630 ppm A 349.2 344.0 345.1 352.0 356.7 361.7 b a Male 630 ppm A 368.5 372.0 350.9 349.9 356.6 358.7 b 3782 Male 630 ppm A 355.7 363.0 335.7 3402 3412 353.1 b 373.6 Male 630 ppm A 333.0 330.7 318.0 3212 333.0 329.7 b a Male 630 ppm A 326.2 328.8 318.1 323.7 3252 3222 b a Male 630 ppm B 356.2 a a a a a a a Male 630 ppm B 354.2 339.6 3492 360.6 371.0 3682 359.0 3972 Male 630 ppm B 334.0 323.7 341.4 346.0 345.4 319.0 310.1 371.0 Male 630 ppm B 297.8 310.4 297.7 289.6 290.9 282.1 273.2 a Male 630 ppm B 335.4 345.8 337.9 333.7 343.4 342.1 322.1 a Male 630 ppm B 337.9 343.0 337.8 3472 345.3 3432 329.2 372.7 Male 630 ppm B 356.4 3603 347.8 358.4 362.6 3642 351.6 Mean SD N 341.9 3393 329.6 332.8 3382 3372 324.2 375.9 17.5 17.7 21.9 27.7 24.1 25.1 312 11.7 14 13 13 13 13 13 6 6 Male 2000 ppm A 334.6 322.4 336.0 339.7 341.1 3302 b 368.6 Male 2000 ppm A 363.5 347.9 345.0 353.7 354.4 364.9 b 380.0 Male 2000 ppm A 363.5 351.1 354.4 359.2 364.4 3732 b a Male 2000 ppm A 373.8 3833 359.4 3742 381.9 390.6 b a Male 2000 ppm A 321.2 3292 312.8 324.4 332.7 334.1 b a Male 2000 ppm A 326.0 328.0 3232 335-0 3372 3302 b a Male 2000 ppm A 309.6 3042 292.7 3022 2962 2992 b 2912 Male 2000 ppm B 370.5 362.6 360.1 368.1 371.9 3502 343.9 a Male 2000 ppm B 348.6 330.6 346.2 3542 360.2 351.7 351.9 390.0 Male 2000 ppm B 304.0 295.7 299.8 310.2 314.4 3092 3002 315.9 Male 2000 ppm B 355.4 358.9 352.6 363.9 375.1 3702 353.1 a Male 2000 ppm B 337.0 3422 331.3 3412 346.9 348.9 329.7 a Male 2000 ppm B 317.7 a a a a a a a Male 2000 ppm B 331.9 333.0 325.1 337.9 347.8 345.6 3282 362.1 Mean SD N 339.8 337.6 333.7 343.4 348.1 346.2 334.6 3512 23.0 23.8 22.1 21.9 242 25.6 19.7 39.0 14 13 13 13 13 13 6 6 aAnimai died; ^Data not collected. 0308 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 2 of 3, Page: 44 Appendix D, continued: Body Weights IE Sex 90A3848 90A3849 90A3850 90A38S1 90A3852 90A38S3 90A3854 90A3855 90A3856 90A3857 90A3858 90A3859 90A3860 90A3861 Female Female Female Female Female Female Female Female Female Female Female Female Female Female Cone Croup lest Pay 3 Z2 6 92 99 m Qppm Oppm Oppm Oppm Oppm Oppm Oppm Oppm Oppm Oppm Oppm Oppm Oppm Oppm A A A A A A A B B B B B B B 183.8 1953 199.9 191.9 192.2 185.4 182.2 186.1 188.1 181.2 189.1 194.4 190.7 176.9 178.2 181.7 192.7 1923 190.9 1892 1825 179.7 180.1 173.7 190.7 191.2 184.7 181.1 181.9 191.9 1963 189.6 187.9 184.2 177.7 185.9 185.8 1793 184.4 192.1 182.6 178.2 1883 1973 2043 196.9 195.1 190.0 189.1 192.9 1883 183.2 189.7 190.1 185.0 1803 1913 2025 2082 199.1 197.0 192.7 1882 196.0 191.9 183.7 190.9 189.7 1865 184.4 183.6 198.1 203.0 2013 200.7 189.4 191.1 197.9 188.4 185.4 1843 195.9 181.4 180.0 b b b b b b b 192.8 188.6 184.7 a 194.0 182.6 176.7 194.1 2045 207.4 196.4 a 196.1 a a a a a a 189.4 184.9 Mean SD N 188.4 184.9 185.6 190.9 193.0 1915 186.6 196.1 6.3 6.2 5.6 6.2 6.9 8.0 6.6 7.9 14 14 14 14 14 14 6 7 90A3862 90A3863 90A3864 90A3865 90A3866 90A3867 90A3868 90A3869 90A3870 90A3871 90A3872 90A3873 90A3874 90A387S Female Female Female Female Female Female Female Female Female Female Female Female Female Female 200 ppm 200 ppm 200 ppm 200 ppm 200 ppm 200 ppm 200 ppm 200 ppm 200 ppm 200 ppm 200 ppm 200 ppm 200 ppm 200 ppm A A A A A A A B B B B B B B 180.3 189.0 188.8 186.0 189.7 187.6 179.1 187.0 1833 183.9 182.2 188.4 180.8 188.8 1783 187.0 183.9 1883 192.9 1892 179.6 1852 182.6 1813 184.6 188.6 1793 1883 1823 1875 1875 184.1 a 1832 175.1 189.1 186.1 1815 177.7 183.6 175.9 183.7 1823 189.1 196.2 185.1 a 1903 182.4 1953 1863 184.7 1805 1895 183.6 192.4 187.9 191.6 188.6 187.9 a 1982 186.4 1995 1892 191.4 180.7 1962 185.0 194.4 186.7 190.0 185.4 1863 a 197.0 1862 191.7 193.6 1935 182.7 1935 186.9 193.9 b b b b a b b 1913 193.1 a 183.1 184.7 184.9 193.0 a 1985 a a a 197.9 185.4 a 193.9 a 188.7 1983 189.8 a Mean SD N 185.4 185.1 182.9 187.6 1905 1893 188.4 193.3 3.7 4.2 4.4 5.1 5.4 43 4.7 5.4 14 14 13 13 13 13 6 7 aAnimal died; ^Data not collected. SL 030881 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 2 of 3, Page: 45 Appendix D, continued: Body Weights m Sex 90A3876 90A3877 90A3878 90A3879 90A3880 90A3881 90A3882 90A3883 90A3884 90A3885 90A3886 90A3887 90A3888 90A3889 Female Female Female Female Female Female Female Female Female Female Female Female Female Female Cone Group 5fi TestCbv & U 79 6 25 22 122 630 ppm 630 ppm 630 ppm 630 ppm 630 ppm 630 ppm 630 ppm 630 ppm 630 ppm 630 ppm 630 ppm 630 ppm 630 ppm 630 ppm A A A A A A A B B B B B B B 195.7 196.0 185.1 193.3 176.1 194.2 188.0 174.2 187.5 169.5 180.1 184.5 191.6 192.1 187.4 186.4 179.4 1963 177.6 1933 189.0 170.6 187.1 1723 1823 1853 191.6 1973 1933 192.4 179.1 1873 175.7 1903 1863 1763 189.6 178.7 1743 1873 194.5 1913 195.6 2073 1813 196.2 179.9 197.4 198.4 179.0 191.6 181.5 179.8 1863 196.6 195.3 2003 208.7 183.7 200.0 184.1 199.6 200.0 1823 199.7 187.7 186.1 197.6 2023 2023 1943 206.1 1843 198.9 1833 198.0 201.1 1803 1973 186.0 181.1 193.7 1993 201.4 b b b b b b b 1793 1833 179.8 177.7 185.2 195.9 1993 206.7 a 190.1 205.1 a 1953 a a 200.6 a a 196.1 205.9 196.9 Mean SD N 186.3 1853 185.6 1903 1953 1933 1853 199.6 83 8.3 7.1 9.0 8.6 83 8.5 6.0 14 14 14 14 14 14 7 8 90A3890 90A3891 90A3892 90A3893 90A3894 90A3895 90A3896 90A3897 90A3898 90A3899 90A3900 90A3901 90A3902 90A3903 Female Female Female Female Female Female Female Female Female Female Female Female Female Female 2000 ppm 2000 ppm 2000 ppm 2000 ppm 2000 ppm 2000 ppm 2000 ppm 2000 ppm 2000 ppm 2000 ppm 2000 ppm 2000 ppm 2000 ppm 2000 ppm A A A A A A A B B B B B B B 185.7 194.1 183.2 196.4 185.0 209.4 193.2 183.2 182.1 191.0 1863 184.1 1883 197.6 179.9 1853 1833 1993 181.7 205.0 197.6 178.4 1763 185.1 186.0 181.7 1883 193.7 1903 188.0 186.4 1923 a 1963 a 186.0 182.4 188.0 183.4 1823 184.6 189.1 197.2 193.1 1883 199.1 a 2043 a 191.2 186.6 186.6 183.9 186.0 189.8 193.4 200.1 1983 195.2 199.4 a 206.6 a 1913 1903 195.7 188.1 1933 197.1 197.6 196.9 1923 1913 200.7 a 2133 a 188.4 187.7 1943 1873 1913 1933 1943 b b b b b b b 181.4 189.0 187.0 1783 1853 1873 183.9 1983 a a a a 199.4 a a 1923 194.7 188.7 a 1903 195.9 Mean SD N 190.0 1'873 187.5 191.6 196.1 194.4 184.8 1923 7.6 83 4.3 6.1 5.0 7.1 3.6 3.0 14 14 12 12 12 12 7 7 aAnimal died; ^Data not collected. SL 030882 SL 030883 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Study ID: K-001716-091 Volume 2 of 3, Page: 46 Appendix E: Functional Observational Battery INDIVIDUAL ANIMAL OBSERVATIONS ANIMAL ID: 90A3792 SEX: MALE SPECIES: RAT CONC: 0 OBSERVATION(S) PPM EYES (PUPIL SI2E, LACKIMATION, ETC.) , t'ECES AND URINE (DECREASED. DARK, MUCOID, REDDISH, ETC.) GENERAL (SALIVATION, OBESE, NASAL DISCHARGE, ETC.) MOVEMENT/BEHAVIOR (INCOORD., MUSC. TONE, AGGRESSIVE, ETC.) RESPIRATION (DEEP, NOISY, SNEEZE, ETC.) SKIN, EUR AND MUCOUS MEMBRANES SENSORY RESPONSIVENESS(TOUCH, 'AUDITORY, NOCICEPTIVE, VISUAL) DAY MONTH MONTH MONTH -Ot 001 002 003 _. --_ _ --_ -- -_ _ __ _ --- - P INDICATES THE OBSERVATION HAS PRESENT - INDICATES NO REMARKABLE OBSERVATIONS 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Study ID: K-001716-091 Volume 2 of 3, Page: 47 Appendix E, continued: Functional Observational Battery INDIVIDUAL ANIMAL OBSERVATIONS ANIMAL ID: 90A3793 SEX; MALE SPECIES: RAT CONC: 0 OBSERVATION(S) PPM DAY MONTH MONTH MONTH -04 001 002 003 EYES (PUPIL SIZE, LACRIMATION, ETC.) FECES AND URINE (DECREASED, DARK, MUCOID, REDDISH, ETC.) GENERAL (SALIVATION, OBESE, NASAL DISCHARGE, ETC.) MOVEMENT/BEHAVIOR (INCOORD., MUSC. TONE, AGGRESSIVE, ETC.) RESPIRATION (DEEP, NOISY, SNEEZE, ETC.) SKIN, FUR AND MUCOUS MEHBRANES SENSORY RESPONSIVENESS(TOUCH, 'AUDITORY, NOCICEPTIVE, VISUAL! DISPOSITION (MISSING, MORIBUND, ETC.) SL 030884 P INDICATES THE OBSERVATION HAS PRESENT - INDICATES NO REMARKABLE OBSERVATIONS S8800 cn 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Study ID: K-001716-091 Volume 2 of 3, Page: 48 Appendix E, continued: Functional Observational Battery INDIVIDUAL ANIMAL OBSERVATIONS ANIMAL ID: 90A3794 SEX: MALE SPECIES: RAT CONC: 0 OBSERVATION(S) PPM DAT MONTH MONTH MONTH -04 001 002 003 EYES (PUPIL SIZE, LACRIHATION, ETC.1 FECES AND URINE (DECREASED, DARK, MUCOID, REDDISH, ETC.) GENERAL (SALIVATION, OBESE, NASAL DISCHARGE, ETC.) MOVEMENT/BEHAVIOR (INCOORD., MUSC. TONE, AGGRESSIVE, ETC.) RESPIRATION (DEEP, NOISY, SNEEZE, ETC.) SKIN, FUR AND MUCOUS MEMBRANES SENSORY RESPONSIVENESS(TOUCH, 'AUDITORY, NOCICEPTIVE, VISUAL) DISPOSITION (MISSING, MORIBUND, ETC.) P INDICATES THE OBSERVATION NAS PRESENT INDICATES NO REMARKABLE OBSERVATIONS INDICATES ANIMAL HAS NOT INCLUDED IN EXAM; ANIMAL STATUS: SP-DEATH DAY 064 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Study ID: K-001716-091 Volume 2 of 3, Page: 49 Appendix E, continued: Functional Observational Battery INDIVIDUAL ANIMAL OBSERVATIONS ANIMAL ID: 90A3795 sex: MALE SPECIES: RAT CONC: 10 OBSERVATION(S) PPM DAY MONTH MONTH MONTH -04 001 002 003 EYES (PUPIL SIZE, LACRIMATION, ETC.) EECES AND URINE (DECREASED, DARK, MUCOID, REDDISH, ETC.) GENERAL (SALIVATION, OBESE, NASAL DISCHARGE, ETC.) MOVEMENT/BEHAVIOR (INCOORD., MUSC. TONE, AGGRESSIVE, ETC.) RESPIRATION (DEEP, NOISY, SNEEZE, ETC.) SKIN, FUR AND MUCOUS MEMBRANES SENSORY RESPONSIVENESS(TOUCH, 'AUDITORY, NOCICEPTIVE, VISUAL) AUDITORY RESPONSIVENESS - DECREASED DISPOSITION (MISSING, MORIBUND, ETC.) - -p - SL 030886 P INDICATES THE OBSERVATION HAS PRESENT - INDICATES NO REMARKABLE OBSERVATIONS 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Study ID: K-001716-091 Volume 2 of 3, Page: 50 Appendix E, continued: Functional Observational Battery INDIVIDUAL ANIMAL OBSERVATIONS ANIMAL ID; 90A3396 SEX: MALE SPECIES: CONC: RAT 0 OBSERVATION(SI PPM DAY MONTH MONTH MONTH -Ot 001 002 003 EKES (PUPIL SIZE, LACKINATION, ETC.) FECES AND URINE (DECREASED, DARK, MUCOID, REDDISH, ETC.) GENERAL (SALIVATION, OBESE, NASAL DISCHARGE, ETC.I MOVEMENT/BEHAVIOR (INCOORD., MUSC. TONE, AGGRESSIVE, ETC.) RESPIRATION (DEEP, NOISY, SNEEZE, ETC.) SKIN, FUR AND MUCOUS MEMBRANES SENSORY RESPONSIVENESS(TOUCH, AUDITORY, NOCICEPTIVE, VISUAL) DISPOSITION (MISSING, MORIBUND, ETC.) SL 030887 P INDICATES THE OBSERVATION HAS PRESENT - INDICATES NO REMARKABLE OBSERVATIONS 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Study ID: K-001716-091 Volume 2 of 3, Page: 51 Appendix E, continued: Functional Observational Battery INDIVIDUAL ANIMAL OBSERVATIONS ANIMAL ID: 90A379I SEX: KALE SPECIES; CONC: RAT i0 OBSERVATION IS) DAT MONTH MONTH MONTH -04 001 002 003 EYES (PUPIL SIZE, LACRIMATION, ETC.) FECES AND URINE (DECREASED, DARK, MUCOID, REDDISH, ETC.) GENERAL (SALIVATION, OBESE, NASAL DISCHARGE, ETC.) MOVEMENT/BEHAVIOR (INCOORD., MUSC. TONE, AGGRESSIVE, ETC.) RESPIRATION (DEEP, NOISY, SNEEZE, ETC.) SKIN, FUR AND HUCOUS MEMBRANES SENSORY RESPONSIVENESS(TOUCH, AUDITORY, NOCICEPTIVE, VISUAL) DISPOSITION (MISSING, MORIBUND, ETC.) SL 030888 P INDICATES THE OBSERVATION MAS PRESENT - INDICATES NO REMARKABLE OBSERVATIONS SL 030889 1,1,1 -T subchronic neurotoxicity The Dow Chemical Company Study ID: K-001716-091 Volume 2 of 3, Page: 52 Appendix E, continued: Functional Observational Battery INDIVIDUAL ANIMAL OBSERVATIONS ANIMAL ID: 90A3I9B SEX: MALE SPECIES: CONC: RAT I0 OBSERVATION(S| PPM DAT MONTH MONTH MONTH -04 001 002 003 EYES (PUPIL SIZE, LACRIMATION, ETC.) FECES AND URINE (DECREASED, DARK, MUCOID, REDDISH, ETC.) GENERAL (SALIVATION, OBESE, NASAL DISCHARGE, ETC.) MOVEMENT/BEHAVIOR (INCOORD., MOSC. TONE, AGGRESSIVE, ETC.) RESPIRATION (DEEP, NOISY, SNEEZE, ETC.) SKIN, FUR AND MUCOUS MEMBRANES SENSORY RESPONSIVENESS(TOUCH, AUDITORY, NOCICEPTIVE, VISUAL) DISPOSITION (MISSING, MORIBUND, ETC.) P INDICATES THE OBSERVATION MAS PRESENT - INDICATES NO REMARKABLE OBSERVATIONS 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Study ID: K-001716-091 Volume 2 of 3, Page: 53 Appendix E, continued: Functional Observational Battery INDIVIDUAL ANIMAL OBSERVATIONS ANIMAL ID: 90A3799 SEX: MALE SPECIES: RAT cunc: 0 OBSERVATION (S) PPM DAY MONTH MONTH MONTH -04 001 002 003 EYES (PUPIL SIZE, LACRIMATION, ETC.* EECES AND URINE (DECREASED, DARK, MUCOID, REDDISH, ETC.) GENERAL (SALIVATION, OBESE, NASAL DISCHARGE, ETC.) MOVEMENT/BEHAVIOR (INCOORD., MUSC. TONE, AGGRESSIVE, ETC.) RESPIRATION (DEEP, NOISY, SNEEZE, ETC.) SKIN, FUR AND MUCOUS MEMBRANES SENSORY RESPONSIVENESS(TOUCH, 'AUDITORY, NOCICEPTIVE, VISUAL) IP DISPOSITION (MISSING, MORIBUND, ETC.) 030890 P INDICATES THE OBSERVATION HAS PRESENT - INDICATES NO REMARKABLE OBSERVATIONS SL 030891 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Study ID: K-001716-091 Volume 2 of 3, Page: 54 Appendix E, continued: Functional Observational Battery INDIVIDUAL ANIMAL OBSERVATIONS ANIMAL ID: 90A3800 SEX: MALE SPECIES: RAT CONC: 0 OBSERVATION(SI PPM DAY MONTH MONTH MONTH -04 001 002 003 EYES (PUPIL SIZE, LACHIMATION, ETC.) FECES AND URINE (DECREASED, DARK, MUCOID, REDDISH, ETC.) GENERAL (SALIVATION, OBESE, NASAL DISCHARGE, ETC.) MOVEMENT/BEHAVIOR (INCOORD., MUSC. TONE, AGGRESSIVE, ETC.) RESPIRATION (DEEP, NOISY, SNEEZE, ETC.) SKIN, FUR AND MUCOUS MEMBRANES SENSORY RESPONSIVENESS(TOUCH,`AUDITORY, NOCICEPTIVE, VISUAL) DISPOSITION (MISSING, MORIBUND, ETC.) _. ---- ---_ -_- -_ -_ -~-_ --- _ --- - P INDICATES THE OBSERVATION NAS PRESENT - INDICATES NO REMARKABLE OBSERVATIONS 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Study ID: K-001716-091 Volume 2 of 3, Page: 55 Appendix E, continued: Functional Observational Battery INDIVIDUAL ANIMAL OBSERVATIONS ANIMAL ID: 90A3801 SEX: MALE SPECIES; CONC: RAT i0 OBSERVATION(S) PPM OAT MONTH MONTH MONTH -04 001 002 003 EYES (PUPIL SIZE, LACRIMATION, ETC.) FECES AND URINE (DECREASED, DARK, MUCOID, REDDISH, ETC.) GENERAL (SALIVATION, OBESE, NASAL DISCHARGE, ETC.) MOVEMENT/BEHAVIOR (INCOORD., MUSC. TONE, AGGRESSIVE, ETC.) RESPIRATION (DEEP, NOISY, SNEEZE, ETC.) SKIN, FUR AND MUCOUS MEMBRANES SENSORY RESPONSIVENESS(TOUCH, 'AUDITORY, NOCICEPTIVE, VISUAL) DISPOSITION (MISSING, MORIBUND, ETC.) SL 030892 P INDICATES THE OBSERVATION HAS PRESENT - INDICATES NO REMARKABLE OBSERVATIONS SL 030893 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Study ID: K-001716-091 Volume 2 of 3, Page: 56 Appendix E, continued: Functional Observational Battery INDIVIDUAL ANIMAL OBSERVATIONS ANIMAL ID: 90A3802 SEX: MALE SPECIES: RAT CONC: 0 OBSERVATION(S) PPM DAY MONTH MONTH MONTH -04 001 002 003 EYES (PUPIL SIZE, LACRIMATION, ETC.) FECES AND URINE (DECREASED, DARK, MUCOID, REDDISH, ETC.) GENERAL (SALIVATION, OBESE, NASAL DISCHARGE, ETC.) MOVEMENT/BEHAVIOR (INCOORO., MUSC. TONE, AGGRESSIVE, ETC.) RESPIRATION (DEEP, NOISY, SNEEZE, ETC.) SKIN, FUR AND MUCOUS MEMBRANES SENSORY RESPONSIVENESS(TOUCH, AUDITORY, NOCICEPTIVE, VISUAL) DISPOSITION (MISSING, MORIBUND, ETC.) P INDICATES THE OBSERVATION HAS PRESENT - INDICATES NO REMARKABLE OBSERVATIONS 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Study ID: K-001716-091 Volume 2 of 3, Page: 57 Appendix E, continued: Functional Observational Battery INDIVIDUAL ANIMAL OBSERVATIONS ANIMAL ID: 90A3803 SEX: MALE SPECIES: RAT CONC: 0 OBSERVATION(S) PPM EKES (PUPIL SIZE, LACRIKATION, ETC.! FECES AND URINE (DECREASED, DARK, MUCOID, REDDISH, ETC.) GENERAL (SALIVATION, OBESE, NASAL DISCHARGE, ETC.) MOVEMENT/BEHAVIOR (INCOORD., HUSC. TONE, AGGRESSIVE, ETC.) RESPIRATION (DEEP, NOISY, SNEEZE, ETC.) SKIN, FUR AND MUCOUS MEMBRANES SENSORY RESPONSIVENESStTOUCH, AUDITORY, NOCICEPTIVE, VISUAL) DISPOSITION (MISSING, MORIBUND, ETC.) DAY MONTH MONTH MONTH -0( 001 002 003 __ _ -- - _ -- _ _ -_ -- _ _ __ _ _ -_ -- - _ _ flOto vO P INDICATES THE OBSERVATION HAS PRESENT - INDICATES NO REMARKABLE OBSERVATIONS SL 030895 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Study ID: K-001716-091 Volume 2 of 3, Page: 58 Appendix E, continued: Functional Observational Battery INDIVIDUAL ANIMAL OBSERVATIONS ANIMAL ID: S0A3804 SEX: MALE SPECIES; RAT conc: 0 OBSERVATION(S) PPM DAY MONTH MONTH MONTH -04 001 002 003 EVES (PUPIL SIZE, LACRIKATION, ETC.) FECES AND URINE (DECREASED, DARK, MUCOID, REDDISH, ETC.) GENERAL (SALIVATION, OBESE, NASAL DISCHARGE, ETC.) MOVEMENT/BEHAVIOR (INCOORD., MUSC. TONE, AGGRESSIVE, ETC.) RESPIRATION (DEEP, NOISY, SNEEZE, ETC.) SKIN, FUR AND MUCOUS MEMBRANES SENSORY RESPONSIVENESS(TOUCH, AUDITORY, NOCICEPTIVE, VISUAL) DISPOSITION (MISSING, MORIBUND, ETC.) P INDICATES THE OBSERVATION HAS PRESENT - INDICATES NO REMARKABLE OBSERVATIONS 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Study ID: K-001716-091 Volume 2 of 3, Page: 59 Appendix E, continued: Functional Observational Battery INDIVIDUAL ANIMAL OBSERVATIONS ANIMAL ID: 9OA30O5 SEX: MALE SPECIES: CONC: RAT 0 OBSERVATION(S) PPM DAT MONTH MONTH MONTH -04 001 002 003 EYES (PUPIL SIZE, LACRIMATION, ETC.) FECES AND URINE (DECREASED, DARK, MUCOID, REDDISH, ETC.) GENERAL (SALIVATION, OBESE, NASAL DISCHARGE, ETC.) MOVEMENT/BEHAVIOR (INCOORD., MUSC. TONE, AGGRESSIVE, ETC.) RESPIRATION (DEEP, NOISY, SNEEZE, ETC.) SKIN, FUR AND MUCOUS MEMBRANES SENSORY RESPONSIVENESS(TOUCH, 'AUDITORY, NOCICEPTIVE, VISUAL) DISPOSITION (MISSING, MORIBUND, ETC.) CO r1 030896 P INDICATES THE OBSERVATION HAS PRESENT - INDICATES NO REMARKABLE OBSERVATIONS SI* 0 3 0 8 9 7 1,1,IT subchronic neurotoxicity The Dow Chemical Company Study ID: K-001716-091 Volume 2 of 3, Page: 60 Appendix E, continued: Functional Observational Battery INDIVIDUAL ANIMAL OBSERVATIONS ANIMAL ID: 90A3806 SEX; MALE SPECIES: RAT CONC; 200 OBSERVATION<S> PPM EKES (PUPIL SIZE, LACRIMATION, ETC.) FECES AND URINE (DECREASED, DARK, MUCOID, REDDISH, ETC.) GENERAL (SALIVATION, OBESE, NASAL DISCHARGE, ETC.) MOVEMENT/BEHAVIOR (INCOORD., MOSC. TONE, AGGRESSIVE, ETC.) RESPIRATION (DEEP, NOISY, SNEEZE, ETC.) SKIN, FUR AND MUCOUS MEMBRANES SENSORY RESPONSIVENESS(TOUCH, 'AUDITORY, NOCICEPTIVE, VISUAL) DISPOSITION (MISSING, MORIBUND, ETC.) DAY MONTH MONTH MONTH -04 001 002 003 ____ ---------- -- - -- ---_ ---- P INDICATES THE OBSERVATION NAS PRESENT - INDICATES NO REMARKABLE OBSERVATIONS 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Study ID: K-001716-091 Volume 2 of 3, Page: 61 Appendix E, continued: Functional Observational Battery INDIVIDUAL ANIMAL OBSERVATIONS ANIMAL ID: 90A380? SEX: MALE SPECIES: RAT CONC: 200 OBSERVATION(S) PPM DAT MONTH MONTH MONTH -04 001 002 003 ETES (PUPIL SIZE, LACKIMATION, ETC.) FECES AND URINE (DECREASED, DARK, MUCOID, REDDISH, ETC.) GENERAL (SALIVATION, OBESE, NASAL DISCHARGE, ETC.) MOVEMENT/BEHAVIOR (INCOORD., MUSC. TONE, AGGRESSIVE, ETC.) RESPIRATION (DEEP, NOIST, SNEEZE, ETC.) SKIN, FUR AND MUCOUS MEMBRANES SENSORY RESPONSIVENESS(TOUCH, AUDITORY, NOCICEPTIVE, VISUAL) DISPOSITION (MISSING, MORIBUND, ETC.) 0o0COo oyoo P INDICATES THE OBSERVATION HAS PRESENT - INDICATES NO REMARKABLE OBSERVATIONS i SL 030899 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Study ID: K-001716-091 Volume 2 of 3, Page: 62 Appendix E, continued: Functional Observational Battery INDIVIDUAL ANIMAL OBSERVATIONS ANIMAL ID: 90A380B SEX: MALE SPECIES: RAT CONC: 200 OBSERVATION<S) PPM DAT MONTH MONTH MONTH -04 001 002 003 ETES (PUPIL SIZE, LACRIMATION, ETC.) FECES AND URINE (DECREASED, DARK, MUCOID, REDDISH, ETC.) GENERAL (SALIVATION, OBESE, NASAL DISCHARGE, ETC.) MOVEMENT/BEHAVIOR (INCOORD., MUSC. TONE, AGGRESSIVE, ETC.) RESPIRATION (DEEP, HOIST, SNEEZE, ETC.) SKIN, FUR AND MUCOUS MEMBRANES SENSORT RESPONSIVENESSITOUCH, 'AUDITOR/, NOCICEPTIVE, VISUAL) AUDITORY RESPONSIVENESS - DECREASED DISPOSITION (MISSING, MORIBUND, ETC.) PP P INDICATES THE OBSERVATION HAS PRESENT - INDICATES NO REMARKABLE OBSERVATIONS 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Study ID: K-001716-091 Volume 2 of 3, Page: 63 Appendix E, continued: Functional Observational Battery INDIVIDUAL ANIMAL OBSERVATIONS ANIMAL ID 90A3809 SEX: MALE SPECIES: RAT CONC: 200 OBSERVATION(S) PPM DAT MONTH MONTH MONTH -04 001 002 003 EYES (PUPIL SIZE, LACRIMATION, ETC.) FECES AND URINE (DECREASED, DARK, MUCOID, REDDISH, ETC.) GENERAL (SALIVATION, OBESE, NASAL DISCHARGE, ETC.) MOVEMENT/BEHAVIOR (INCOORD., MDSC. TONE, AGGRESSIVE, ETC.) RESPIRATION (DEEP, NOISY, SNEEZE, ETC.) SKIN, FUR AND MUCOUS MEMBRANES SENSORY RESPONSIVENESS(TOUCH, 'AUDITORY, NOCICEPTIVE, VISUAL) DISPOSITION (MISSING, MORIBUND, ETC.) SL 030900 P INDICATES THE OBSERVATION HAS PRESENT - INDICATES NO REMARKABLE OBSERVATIONS SL 030901 1,1,1 -T subchronic neurotoxicity The Dow Chemical Company Study ID: K-001716-091 Volume 2 of 3, Page: 64 Appendix E, continued: Functional Observational Battery INDIVIDUAL ANIMAL OBSERVATIONS ANIMAL ID 9OA301O SEX: MALE SPECIES: RAT conc: 200 OBSERVATION(S) PPM DAY MONTH MONTH MONTH -04 001 002 003 EYES (PUPIL SIZE, LACRIMATION, ETC.) EECES AND URINE (DECREASED, DARK, MUCOID, REDDISH, ETC.) GENERAL (SALIVATION, OBESE, NASAL DISCHARGE, ETC.) THIN MOVEMENT/BEHAVIOR (INCOORD., MUSC. TONE, AGGRESSIVE, ETC.) RESPIRATION (DEEP, NOISY, SNEEZE, ETC.I SKIN, FUR AND MUCOUS MEMBRANES SENSORY RESPONSIVENESS1TOUCH, AUDITORY, NOCICEPTIVE, VISUAL) DISPOSITION (MISSING, MORIBUND, ETC.) P P INDICATES THE OBSERVATION HAS PRESENT - INDICATES NO REMARKABLE OBSERVATIONS 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Study ID: K-001716-09I Volume 2 of 3, Page: 65 Appendix E, continued: Functional Observational Battery INDIVIDUAL ANIMAL OBSERVATIONS ANIMAL ID: 90A381I SEX: MALE SPECIES: RAT CONC: 200 OBSERVATION<S) PPM DAY MONTH MONTH MONTH -04 001 002 003 EYES (PUPIL SIZE, LACRIKATION, ETC.) FECES AND URINE (DECREASED, DARK, MUCOID, REDDISH, ETC.) GENERAL (SALIVATION, OBESE, NASAL DISCHARGE, ETC.) MOVEMENT fBEHAVIOR (INCOORD., MUSC. TONE, AGGRESSIVE, ETC.) RESPIRATION (DEEP, NOISY, SNEEZE, ETC.) SKIN, FUR AND MUCOUS MEMBRANES SENSORY RESPONSIVENESS(TOUCH, 'AUDITORY, NOCICEPTIVE, VISUAL) DISPOSITION (MISSING, MORIBUND, ETC.) SL 030902 P INDICATES THE OBSERVATION HAS PRESENT - INDICATES NO REMARKABLE OBSERVATIONS 1 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Study ID: K-001716-091 Volume 2 of 3, Page: 66 Appendix E, continued: Functional Observational Battery INDIVIDUAL ANIMAL OBSERVATIONS ANIMAL ID: 90A3B12 SEX; MALE SPECIES: RAT CONC: 200 OBSERVATION(S) PPM EYES (PUPIL SIZE, LACRIMATION, ETC.) FECES AND URINE (DECREASED, DARK, MUCOID, REDDISH, ETC.) GENERAL (SALIVATION, OBESE, NASAL DISCHARGE, ETC.) MOVEMENT/BEHAVIOR (INCOORD., MUSC. TONE, AGGRESSIVE, ETC.) RESPIRATION (DEEP, NOISY, SNEEZE, ETC.I SKIN, FUR AND MUCOUS MEMBRANES SENSORY RESPONSIVENESS(TOUCH, AUDITORY, NOCICEPTIVE, VISUAL) DISPOSITION (MISSING, MORIBUND, ETC.) DAY MONTH MONTH MONTH -04 001 002 003 __ _ --_ _ -- _ _ __ --_ -_ __ _- _ -- - _ SL 030903 P INDICATES THE OBSERVATION WAS PRESENT - INDICATES NO REMARKABLE OBSERVATIONS 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Study ID: K-001716-091 Volume 2 of 3, Page: 67 Appendix E, continued: Functional Observational Battery INDIVIDUAL ANIMAL OBSERVATIONS ANIMAL ID: 90A3813 SEX: MALE SPECIES: RAT CONC; 200 OBSERVATION IS) PPM DAY MONTH MONTH MONTH -04 001 002 003 EYES (PUPIL SIZE, LACRIMATION, ETC.) FECES AND URINE (DECREASED, DARK, MUCOID, REDDISH, ETC.) GENERAL (SALIVATION, OBESE, NASAL DISCHARGE, ETC.) MOVEMENT/BEHAVIOR (INCOORD., MUSC. TORE, AGGRESSIVE, ETC.) RESPIRATION (DEEP, NOISY, SNEEZE, ETC.) SKIN, FUR AND MUCOUS MEMBRANES SENSORY RESPONSIVENESSlTOUCH, 'AUDITORY, NOCICEPTIVE, VISUAL) DISPOSITION (MISSING, MORIBUND, ETC. I SL 030904 P INDICATES THE OBSERVATION HAS PRESENT - INDICATES NO REMARKABLE OBSERVATIONS Gj o VoCOo Oot 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Study ID: K-001716-091 Volume 2 of 3, Page: 68 Appendix E, continued: Functional Observational Battery INDIVIDUAL ANIMAL OBSERVATIONS ANIMAL ID; 90A38I4 SEX: MALE SPECIES; RAT CONC: 200 OBSERVATION(S) PPM DAY MONTH MONTH MONTH -04 001 002 003 EYES (PUPIL SIZE, LACRIMATION, ETC.) FECES AND URINE (DECREASED, DARK, MUCOID, REDDISH, ETC.) GENERAL (SALIVATION, OBESE, NASAL DISCHARGE, ETC.) MOVEMENT/BEHAVIOR (INCOORD., MUSC. TONE, AGGRESSIVE. ETC.) RESPIRATION (DEEP, NOISY, SNEEZE, ETC.) SKIN, FUR AND MUCOUS MEMBRANES SENSORY RESPONSIVENESS(TOUCH, `AUDITORY, NOCICEPTIVE, VISUAL) DISPOSITION (MISSING, MORIBUND, ETC.) P INDICATES THE OBSERVATION HAS PRESENT - INDICATES NO REMARKABLE OBSERVATIONS 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Study ID: K-001716-091 Volume 2 of 3, Page: 69 Appendix E, continued: Functional Observational Battery INDIVIDUAL ANIMAL OBSERVATIONS ANIMAL ID: 90A3815 SEX: MALE SPECIES: RAT COHC: 200 OBSERVATION(S) PPM DAY MONTH MONTH MONTH -04 001 002 003 EYES (PUPIL SIZE, LACRIMATION, ETC.) FECES AND URINE (DECREASED, DARK, MUCOID, REDDISH, ETC.) GENERAL (SALIVATION, OBESE, NASAL DISCHARGE, ETC.) MOVEMENT/BEHAVIOR (INCOORO., KUSC. TONE, AGGRESSIVE, ETC.) RESPIRATION (DEEP, NOISY, SNEEZE, ETC.) SKIN, FUR AND MUCOUS MEMBRANES SENSORY RESPONSIVENESS(TOUCH, AUDITORY, NOCICEPTIVE, VISUAL) DISPOSITION (MISSING, MORIBUND, ETC.) CO 9 600 P INDICATES THE OBSERVATION NAS PRESENT - INDICATES NO REMARKABLE OBSERVATIONS 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Study ID: K-001716-091 Volume 2 of 3, Page: 70 Appendix E, continued; Functional Observational Battery INDIVIDUAL ANIMAL OBSERVATIONS ANIMAL ID: 90A3816 SEX; MALE SPECIES: RAT CONC: 200 OBSERVATION(S) PPM DAY MONTH MONTH MONTH -04 001 002 00 3 EYES (PUPIL SIZE, LACRIMATION, ETC.) FECES AND URINE (DECREASED, DARK, MUCOID, REDDISH, ETC.) GENERAL (SALIVATION, OBESE, NASAL DISCHARGE, ETC.) MOVEMENT/BEHAVIOR (INCOORD., MUSC. TONE, AGGRESSIVE, ETC.) RESPIRATION (DEEP, NOISY, SNEEZE, ETC.) SKIN, EUR AND MUCOUS MEMBRANES SENSORY RESPONSIVENESS(TOUCH, 'AUDITORY, NOCICEPTIVE, VISUALI DISPOSITION (MISSING, MORIBUND, ETC.) __ _ _ -- - -- - -- - -- - -- - -- - -- - - P INDICATES THE OBSERVATION HAS PRESENT - INDICATES NO REMARKABLE OBSERVATIONS 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Study ID: K-001716-091 Volume 2 of 3, Page: 71 Appendix E, continued: Functional Observational Battery INDIVIDUAL ANIMAL OBSERVATIONS ANIMAL ID: 90A38IT SEX: MALE SPECIES: RAT CONC: 200 OBSERVATION IS) PPM DAT MONTH MONTH MONTH -04 001 002 003 EYES (PUPIL SIZE, LACRIMATION, ETC.) FECES AND URINE (DECREASED, DARK, MUCOID, REDDISH, ETC.) GENERAL (SALIVATION, OBESE, NASAL DISCHARGE, ETC.) MOVEMENT/BEHAVIOR (INCOORD., MUSC. TONE, AGGRESSIVE, ETC.) RESPIRATION (DEEP, NOISY, SNEEZE, ETC.) SKIN, FUR AND MUCOUS MEMBRANES SENSORY RESPONSIVENESS(TOUCH,'AUDITORY, NOCICEPTIVE, VISUAL) DISPOSITION (MISSING, MORIBUND, ETC.) 030908 P INDICATES THE OBSERVATION HAS PRESENT - INDICATES NO REMARKABLE OBSERVATIONS SL 030909 1,1,l-T subchronic neurotoxicity The Dow Chemical Company Study ID: K-001716-091 Volume 2 of 3, Page: 72 Appendix E, continued: Functional Observational Battery INDIVIDUAL ANIMAL OBSERVATIONS ANIMAL ID: 90A381B SEX: MALE SPECIES: RAT CONC: 200 OBSERVATION(S) PPM DAY MONTH MONTH MONTH -04 001 002 003 EYES (PUPIL SIZE, LACRIMATION, ETC.) FECES AND URINE (DECREASED, DARK, MUCOID, REDDISH, ETC.) GENERAL (SALIVATION, OBESE, NASAL DISCHARGE, ETC.) MOVEMENT/BEHAVIOR (INCOORD., MUSC. TONE, AGGRESSIVE, ETC.) RESPIRATION (DEEP, NOISY, SNEEZE, ETC.) SKIN, FUR AND MUCOUS MEMBRANES SENSORY RESPONSIVENESS(TOUCH, AUDITORY, NOCICEPTIVE, VISUAL) DISPOSITION (MISSING, MORIBUND, ETC.) P INDICATES THE OBSERVATION HAS PRESENT - INDICATES NO REMARKABLE OBSERVATIONS 1,1,1 -T subchronic neurotoxicity The Dow Chemical Company Study ID: K-001716-091 Volume 2 of 3, Page: 73 Appendix E, continued: Functional Observational Battery INDIVIDUAL ANIMAL OBSERVATIONS ANIMAL ID: 90A3819 SEX: MALE SPECIES: RAT CONC; 200 OBSERVATION IS) PPM DAY MONTH MONTH MONTH -04 001 002 003 EYES (PUPIL SIZE, LACRINATION, ETC.) FECES AND URINE (DECREASED, DARK, MUCOID, REDDISH, ETC.) GENERAL (SALIVATION, OBESE, NASAL DISCHARGE, ETC.) MOVEMENT/BEHAVIOR (INCOORD., MUSC. TONE, AGGRESSIVE, ETC.) RESPIRATION (DEEP, NOISY, SNEEZE, ETC.) SKIN, FUR AND MUCOUS MEMBRANES SENSORY RESPONSIVENESSITOUCH, 'AUDITORY, NOCICEPTIVE, VISUAL) DISPOSITION (MISSING, MORIBUND, ETC.) __ _ -- - - *- - - -- - - -- - - -- -_ -- - -- -_ in V* 030910 P INDICATES THE OBSERVATION NAS PRESENT - INDICATES NO REMARKABLE OBSERVATIONS SL 030911 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Study ID: K-001716-091 Volume 2 of 3, Page: 74 Appendix E, continued: Functional Observational Battery INDIVIDUAL ANIMAL OBSERVATIONS ANIMAL ID: 9OA302O SEX: MALE SPECIES: RAT CONC: 630 OBSERVATION(S) PPM EYES (PUPIL SIZE, LACKIMATION, ETC.) EECES AND URINE (DECREASED, DARK, MUCOID, REDDISH, ETC.) GENERAL (SALIVATION, OBESE, NASAL DISCHARGE, ETC.) MOVEMENT/BEHAVIOR (INCOORD., MUSC. TONE, AGGRESSIVE, ETC.) RESPIRATION (DEEP, NOISY, SNEEZE, ETC.) SKIN, FUR AND MUCOUS MEMBRANES SENSORY RESPONSIVENESS(TOUCH, `AUDITORY, NOCICEPTIVE, VISUAL) DISPOSITION (MISSING, MORIBUND, ETC.) DAY MONTH MONTH MONTH -04 001 002 003 __ _ _ -- - -------- --- - - -- - -- - - P INDICATES THE OBSERVATION HAS PRESENT - INDICATES NO REMARKABLE OBSERVATIONS 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Study ID: K-001716-091 Volume 2 of 3, Page: 75 Appendix E, continued: Functional Observational Battery INDIVIDUAL ANIMAL OBSERVATIONS ANIMAL ID: 90A3B21 SEX: MALE SPECIES: RAT CONC: 630 OBSERVATION(S) PPM DAY MONTH MONTH MONTH -04 001 002 003 EVES {PUPIL SIZE, LACRIMATION, ETC.) FECES AND URINE (DECREASED, DARK, MUCOID, REDDISH, ETC.) GENERAL (SALIVATION, OBESE, NASAL DISCHARGE, ETC.) MOVEMENT/BEHAVIOR (INCOORD., MUSC. TONE, AGGRESSIVE, ETC.) RESPIRATION {DEEP, NOISY, SNEEZE, ETC.) SKIN, FUR AND MUCOUS MEMBRANES SENSORY RESPONSIVENESS(TOUCH, `AUDITORY, NOCICEPTIVE, VISUAL) DISPOSITION (MISSING, MORIBUND, ETC.) SL 030912 P INDICATES THE OBSERVATION WAS PRESENT - INDICATES NO REMARKABLE OBSERVATIONS SL 030913 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Study ID: K-001716-091 Volume 2 of 3, Page: 76 Appendix E, continued: Functional Observational Battery INDIVIDUAL. ANIMAL OBSERVATIONS ANIMAL ID: 90A3822 SEX: MALE SPECIES: RAT CONC: 630 OBSERVATION(S) PPM DAT MONTH MONTH MONTH -04 001 002 003 EYES (PUPIL SIZE, LACRIMATION, ETC.} FECES AND URINE (DECREASED, DARK, MUCOID, REDDISH, ETC.) GENERAL (SALIVATION, OBESE, NASAL DISCHARGE, ETC.) MOVEMENT/BEHAVIOR (INCOORD., MUSC. TONE, AGGRESSIVE, ETC.) RESPIRATION (DEEP, NOISY, SNEEZE, ETC.) SKIN, FUR AND MUCOUS MEMBRANES SENSORY RESPONSIVENESS(TOUCH,'AUDITORY, NOCICEPTIVE, VISUAL) DISPOSITION (MISSING, MORIBUND, ETC.) P INDICATES THE OBSERVATION HAS PRESENT - INDICATES NO REMARKABLE OBSERVATIONS 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Study ID: K-001716-091 Volume 2 of 3, Page: 77 Appendix E, continued: Functional Observational Battery INDIVIDUAL ANIMAL OBSERVATIONS ANIMAL ID: 90A3823 SEX: MALE SPECIES: RAT CONC: 630 OBSERVATION(S) PPM DAY MONTH MONTH MONTH -04 001 002 003 EYES (PUPIL SIZE, LACRIMATION, ETC.) FECES AND URINE (DECREASED, DARK, MUCOID, REDDISH, ETC.) GENERAL (SALIVATION, OBESE, NASAL DISCHARGE, ETC.) MOVEMENT/BEHAVIOR (INCOORO., HUSC. TONE, AGGRESSIVE, ETC.) RESPIRATION (DEEP, NOISY, SNEEZE, ETC.) SKIN, FUR AND MUCOUS MEMBRANES SENSORY RESPONSIVENESS(TOUCH,'AUDITORY, NOCICEPTIVE, VISUAL) DISPOSITION (MISSING, MORIBUND, ETC.) SL 030914 P INDICATES THE OBSERVATION HAS PRESENT - INDICATES NO REMARKABLE OBSERVATIONS SL 030915 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Study ID: K-001716-091 Volume 2 of 3, Page: 78 Appendix E, continued: Functional Observational Battery INDIVIDUAL ANIMAL OBSERVATIONS ANIMAL ID; 90A3B24 SEX: MALE SPECIES: RAT cunc: 630 OBSERVATION IS) PPH DA1 MONTH MONTH MONTH -04 001 002 003 EKES (PUPIL SIZE, LACRIMATION, ETC.) FECES AND URINE (DECREASED, DARK, MUCOID, REDDISH, ETC.) GENERAL (SALIVATION, OBESE, NASAL DISCHARGE, ETC.) MOVEMENT/BEHAVIOR (INCOORD., MUSC. TONE, AGGRESSIVE, ETC.) RESPIRATION (DEEP, NOISY, SNEEZE, ETC.) SKIN, FUR AND MUCOUS MEMBRANES SENSORY RESPONSIVENESS (TOUCH, 'AUDITORY, NOCICEPTIVE, VISUAL) DISPOSITION (MISSING, MORIBUND, ETC.) P INDICATES THE OBSERVATION WAS PRESENT - INDICATES NO REMARKABLE OBSERVATIONS 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Study ID: K-001716-091 Volume 2 of 3, Page: 79 Appendix E, continued: Functional Observational Battery INDIVIDUAL ANIMAL OBSERVATIONS ANIMAL ID: 90A3825 SEX: MALE SPECIES; RAT CONC: 630 OBSERVATION(S} DAT MONTH MONTH MONTH -04 001 002 003 EYES (PUPIL SIZE, LACRIMATION, ETC.) FECES AND URINE (DECREASED, DARK, MUCOID, REDDISH, ETC.) GENERAL (SALIVATION, OBESE, NASAL DISCHARGE, ETC.) MOVEMENT/BEHAVIOR (INCOORD., MUSC. TONE, AGGRESSIVE, ETC.) RESPIRATION (DEEP, NOISY, SNEEZE, ETC.) SKIN, FUR AND MUCOUS MEMBRANES SENSORY RESPONSIVENESS(TOUCH,'AUDITORY, NOCICEPTIVE, VISUAL) DISPOSITION (MISSING, MORIBUND, ETC.) SL 030916 P INDICATES THE OBSERVATION MAS PRESENT - INDICATES NO REMARKABLE OBSERVATIONS SL 030917 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Study ID: K-001716-091 Volume 2 of 3, Page: 80 Appendix E, continued: Functional Observational Battery INDIVIDUAL ANIMAL OBSERVATIONS ANIMAL ID: 90A3826 SEX; MALE SPECIES: RAT CONC: 630 OBSERVATION(S) PPM DAY MONTH MONTH MONTH -04 001 002 003 EYES (PUPIL SIZE, LACRIMATION, ETC.) FECES ANO URINE (DECREASED, DARK, MUCOID, REDDISH, ETC.) GENERAL (SALIVATION, OBESE, NASAL DISCHARGE, ETC.) MOVEMENT/BEHAVIOR (INCOORD., MUSC. TONE, AGGRESSIVE, ETC.) RESPIRATION (DEEP, NOISY, SNEEZE, ETC.) SKIN, FUR AND MUCOUS MEMBRANES SENSORY RESPONSIVENESS(TOUCH, 'AUDITORY, NOCICEPTIVE, VISUAL) DISPOSITION (MISSING, MORIBUND, ETC.) P INDICATES THE OBSERVATION NAS PRESENT - INDICATES NO REMARKABLE OBSERVATIONS 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Study ID: K-001716-091 Volume 2 of 3, Page: 81 Appendix E, continued: Functional Observational Battery INDIVIDUAL ANIMAL OBSERVATIONS ANIMAL ID: 90A3827 SEX: MALE SPECIES: RAT CONC: 630 OBSERVATION(S) PPM DAT MONTH MONTH MONTH -04 00] 002 003 EYES (PUPIL SIZE, LACRIMATION, ETC.) FECES AND URINE (DECREASED, DARK, MUCOID, REDDISH, ETC.) GENERAL (SALIVATION, OBESE, NASAL DISCHARGE, ETC.) MOVEMENT/BEHAVIOR (INCOORD., MUSC. TONE, AGGRESSIVE, ETC.) RESPIRATION (DEEP, NOISY, SNEEZE, ETC.) SKIN, FUR AND MUCOUS MEMBRANES SENSORY RESPONSIVENESS(TOUCH, AUDITORY, NOCICEPTIVE, VISUAL) DISPOSITION (MISSING, MORIBUND, ETC.) SL 030918 P INDICATES THE OBSERVATION HAS PRESENT - INDICATES NO REMARKABLE OBSERVATIONS * INDICATES ANIMAL HAS NOT INCLUDED IN' EXAM; ANIMAL STATUS: SP-DEATH DAY 063 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Study ID: K-001716-091 Volume 2 of 3, Page: 82 Appendix E, continued: Functional Observational Battery INDIVIDUAL ANIMAL OBSERVATIONS ANIMAL ID: 90A3B28 SEX: MALE SPECIES: RAT CONC: 630 OBSERVATION<S> PPM DAY MONTH MONTH MONTH -04 001 002 003 EYES (PUPIL SIZE, LACRIMATION, ETC.) FECES AND URINE (DECREASED, DARK, HUCOID, REDDISH, ETC.) GENERAL (SALIVATION, OBESE, NASAL DISCHARGE, ETC.) MOVEMENT/BEHAVIOR UNCOORD., MUSC. TONE, AGGRESSIVE, ETC.) RESPIRATION (DEEP, NOISY, SNEEZE, ETC.) SKIN, FUR AND MUCOUS MEMBRANES SENSORY RESPONSIVENESS(TOUCH, AUDITORY, NOCICEPTIVE, VISUAL) DISPOSITION (MISSING, MORIBUND, ETC.) SL 030919 P INDICATES THE OBSERVATION HAS PRESENT - INDICATES NO REMARKABLE OBSERVATIONS 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Study ID: K-001716-091 Volume 2 of 3, Page: 83 Appendix G, continued: Functional Observational Battery INDIVIDUAL ANIMAL OBSERVATIONS ANIMAL ID: 90A3829 SEX: MALE SPECIES: RAT CONC: 630 OBSERVATION(S) PPM DAY MONTH MONTH MONTH -04 001 002 003 EYES (PUPIL SIZE, LACRIMATION, ETC.) FECES AND URINE (DECREASED, DARK, MUCOID, REDDISH, ETC.) GENERAL (SALIVATION, OBESE, NASAL DISCHARGE, ETC.) MOVEMENT/BEHAVIOR (INCOORD., MUSC. TONE, AGGRESSIVE, ETC.) RESPIRATION (DEEP, NOISY, SNEEZE, ETC.) SKIN, FUR AND MUCOUS MEMBRANES SENSORY RESPONSIVENESS(TOUCH, 'AUDITORY, NOCICEPTIVE, VISUAL) DISPOSITION (MISSING, MORIBUND, ETC.) SL 030920 P INDICATES THE OBSERVATION HAS PRESENT - INDICATES NO REMARKABLE OBSERVATIONS 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Study ID: K-001716-091 Volume 2 of 3, Page: 84 Appendix E, continued: Functional Observational Battery INDIVIDUAL ANIMAL OBSERVATIONS ANIMAL ID; 90A3B30 SEX: MALE SPECIES: RAT CONC: 630 OBSERVATION(S) PPM DAT MONTH MONTH MONTH -04 001 002 003 EYES (PUPIL SIZE, LACRIMATION, ETC. FECES AND URINE (DECREASED, DARK, MUCOID, REDDISH, ETC.) GENERAL (SALIVATION, OBESE, NASAL DISCHARGE, ETC.) MOVEMENT/BEHAVIOR UNCOORD., MUSC, TONE, AGGRESSIVE, ETC.) RESPIRATION (DEEP, NOISY, SNEEZE, ETC.) SKIN, FUR AND MUCOUS MEMBRANES SENSORY RESPONSIVENESS(TOUCH, `AUDITORY, NOCICEPTIVE, VISUAL) DISPOSITION (MISSING, MORIBUND, ETC.) SL 030921 P INDICATES THE OBSERVATION HAS PRESENT - INDICATES NO REMARKABLE OBSERVATIONS 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Study ID: K-001716-091 Volume 2 of 3, Page: 85 Appendix E, continued: Functional Observational Battery INDIVIDUAL ANIMAL OBSERVATIONS ANIMAL ID: 90A3831 SEX: MALE SPECIES: RAT CONC: 630 OBSERVATION(St PPM DAY MONTH MONTH MONTH -04 001 002 003 EYES (PUPIL SIZE, LACRIMATION, ETC.) FECES AND URINE (DECREASED, DARK, MUCOID, REDDISH, ETC.I GENERAL (SALIVATION, OBESE, NASAL DISCHARGE, ETC.) MOVEMENT/BEHAVIOR (INCOORD., MUSC. TONE, AGGRESSIVE, ETC.) RESPIRATION (DEEP, NOISY, SNEEZE, ETC.) SKIN, FUR AND MUCOUS MEMBRANES SENSORY RESPONSIVENESS(TOUCH, 'AUDITORY, NOCICEPTIVE, VISUAL) DISPOSITION (MISSING, MORIBUND, ETC.) SL 030922 P INDICATES THE OBSERVATION MAS PRESENT - INDICATES NO REMARKABLE OBSERVATIONS SL 030923 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Study ID: K-001716-091 Volume 2 of 3, Page: 86 Appendix E, continued: Functional Observational Battery INDIVIDUAL ANIMAL OBSERVATIONS ANIMAL ID: 90A3832 SEX: MALE SPECIES: RAT CONC: 630 OBSERVATIONS) PPM DAT MONTH MONTH MONTH -0* 001 002 003 E*ES (PUPIL SIZE, LACRIMATION, ETC.) FECES AND URINE (DECREASED, DARK, MUCOID, REDDISH, ETC.) GENERAL (SALIVATION, OBESE, NASAL DISCHARGE, ETC.) MOVEMENT/BEHAVIOR (INCOORD., MUSC. TONE, AGGRESSIVE, ETC.) RESPIRATION (OEEP, NOIS*, SNEEZE, ETC.) SKIN, FUR AND MUCOUS MEMBRANES SENSOR* RESPONSIVENESS(TOUCH, 'AUDITOR*, NOCICEPTIVE, VISUAL) DISPOSITION (MISSING, MORIBUND, ETC.) P INDICATES THE OBSERVATION HAS PRESENT - INDICATES NO REMARKABLE OBSERVATIONS 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Study ID: K-001716-091 Volume 2 of 3, Page: 87 Appendix E, continued: Functional Observational Battery INDIVIDUAL ANIMAL OBSERVATIONS ANIMAL ID: 90A3833 SEX: MALE SPECIES: RAT conc: 630 OBSERVATION(S) PPM DAT MONTH MONTH MONTH -04 001 002 003 EYES IPUPIL SIZE, LACR1MATIOH, ETC.) FECES AND URINE (DECREASED, DARK, MUCOID, REDDISH, ETC.) GENERAL (SALIVATION, OBESE, NASAL DISCHARGE, ETC.) MOVEMENT/BEHAVIOR (INCOORD., MUSC. TONE, AGGRESSIVE, ETC.) RESPIRATION (DEEP, NOISY, SNEEZE, ETC.) SKIN, FUR AND MUCOUS MEMBRANES SENSORY RESPONSIVENESS(TOUCH, 'AUDITORY, NOCICEPTIVE, VISUAL) DISPOSITION (MISSING, MORIBUND, ETC.) LO r1 vteoto P INDICATES THE OBSERVATION HAS PRESENT - INDICATES NO REMARKABLE OBSERVATIONS SL 030925 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Study ID: K-001716-091 Volume 2 of 3, Page: 88 Appendix E, continued: Functional Observational Battery ANIMAL ID: 90A3B34 SEX: MALE SPECIES: RAT CONC: 2000 OBSERVATION{S) PPM INDIVIDUAL ANIMAL OBSERVATIONS DAT MONTH MONTH MONTH -04 001 002 003 EYES (PUPIL SIZE, LACRIMATION, ETC.) FECES AND URINE (DECREASED, DARK, MUCOID, REDDISH, ETC.) GENERAL (SALIVATION, OBESE, NASAL DISCHARGE, ETC.) MOVEMENT/BEHAVIOR (INCOORD., MUSC. TONE, AGGRESSIVE, ETC.) RESPIRATION (DEEP, NOISY, SNEEZE, ETC.) RESPIRATION SLOW SKIN, FUR AND MUCOUS MEMBRANES) SENSORY RESPONSIVENESS(TOUCH, AUDITORY, NOCICEPTIVE, VISUAL) DISPOSITION (MISSING, MORIBUND, ETC.) -p P INDICATES THE OBSERVATION HAS PRESENT - INDICATES NO REMARKABLE OBSERVATIONS 1,1,1 -T subchronic neurotoxicity The Dow Chemical Company Study ID: K-001716-091 Volume 2 of 3, Page: 89 Appendix E, continued: Functional Observational Battery INDIVIDUAL. ANIMAL OBSERVATIONS ANIMAL ID: 90A3B35 SEX: MALE SPECIES: RAT CONC: 2000 OBSERVATION IS) PPM DAY MONTH MONTH MONTH -04 001 002 003 EYES (PUPIL SI2E, LACRIMATION, ETC.) FECES AND URINE (DECREASED, DARK, MUCOID, REDOISH, ETC.| GENERAL (SALIVATION, OBESE, NASAL DISCHARGE, ETC.) MOVEMENT/BEHAVIOR (INCOORD., MUSC. TONE, AGGRESSIVE, ETC.) RESPIRATION (DEEP, NOISY, SNEEZE, ETC.) SKIN, FUR AND MUCOUS MEMBRANES SENSORY RESPONSIVENESSITOUCH, AUDITORY, NOCICEPTIVE, VISUAL) DISPOSITION (MISSING, MORIBUND, ETC.) SL 030926 P INDICATES THE OBSERVATION MAS PRESENT - INDICATES NO REMARKABLE OBSERVATIONS 030927 V* 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Study ID: K-001716-091 Volume 2 of 3, Page: 90 Appendix E, continued: Functional Observational Battery INDIVIDUAL ANIMAL OBSERVATIONS ANIMAL ID: 90A3S36 SEX: MALE SPECIES: RAT conc: 2000 OBSERVATION IS) PPM DAK MONTH MONTH MONTH -04 001 002 003 EYES (PUPIL SIZE, LACRIMATION, ETC.) FECES AND URINE (DECREASED, DARK, MUCOID, REDDISH, ETC.) GENERAL (SALIVATION, OBESE, NASAL DISCHARGE, ETC.) MOVEMENT/BEHAVIOR (INCOORD., MUSC. TONE, AGGRESSIVE, ETC.) RESPIRATION (DEEP, NOISY, SNEEZE, ETC.) SKIN, FUR AND MUCOUS MEMBRANES SENSORY RESPONSIVENESS(TOUCH, AUDITORY, NOCICEPTIVE, VISUAL) DISPOSITION (MISSING, MORIBUND, ETC.) P INDICATES THE OBSERVATION HAS PRESENT - INDICATES NO REMARKABLE OBSERVATIONS 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Study ID: K-001716-091 Volume 2 of 3, Page: 91 Appendix E, continued: Functional Observational Battery INDIVIDUAL ANIMAL OBSERVATIONS ANIMAL ID: 90A3B37 SEX: MALE SPECIES: RAT CONC: 2000 OBSERVATION(S) PPM DAT MONTH MONTH MONTH -04 001 002 003 EYES (PUPIL SIZE, LACRIHATION, ETC.) FECES AND URINE (DECREASED, DARK, MUCOID, REDDISH, ETC.) GENERAL (SALIVATION, OBESE, NASAL DISCHARGE, ETC.) MOVEMENT/BEHAVIOR (INCOORD., MUSC. TONE, AGGRESSIVE, ETC.) RESPIRATION (DEEP, NOISY, SNEEZE, ETC.) SKIN, FUR AND MUCOUS MEMBRANES SENSORY RESPONSIVENESS(TOUCH, 'AUDITORY, NOCICEPTIVE, VISUAL) DISPOSITION (MISSING, MORIBUND, ETC.) SL 030928 P INDICATES THE OBSERVATION HAS PRESENT - INDICATES NO REMARKABLE OBSERVATIONS SL 030929 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Study ID: K-001716-091 Volume 2 of 3, Page: 92 Appendix E, continued: Functional Observational Battery INDIVIDUAL ANIMAL OBSERVATIONS ANIMAL ID: 90A383B SEX: MALE SPECIES: RAT CONC: 2000 OBSERVATION<S) PPM EYES (PUPIL SIZE, LACRIMATION, ETC.) FECES AND URINE (DECREASED, DARK, MUCOID, REDDISH, ETC.) GENERAL {SALIVATION, OBESE, NASAL DISCHARGE, ETC.) MOVEMENT/BEHAVIOR lINCOORD., MUSC. TONE, AGGRESSIVE, ETC.) RESPIRATION (OEEP, NOISY, SNEEZE, ETC.) SKIN, FUR AND MUCOUS MEMBRANES SENSORY RESPONSIVENESS{TOUCH, `AUDITORY, NOCICEPTIVE, VISUAL) DISPOSITION (MISSING, MORIBUND, ETC.) DAY MONTH MONTH MONTH -or 001 002 003 __ - - --- --- -_ -- _ - - --- - -- -- P INDICATES THE OBSERVATION HAS PRESENT - INDICATES NO REMARKABLE OBSERVATIONS 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Study ID: K-001716-091 Volume 2 of 3, Page: 93 Appendix E, continued: Functional Observational Battery INDIVIDUAL ANIMAL OBSERVATIONS ANIMAL ID; 90A3839 SEX: MALE SPEC IES: RAT CONC; 2000 OBSERVATION(S) PPM DAY MONTH MONTH MONTH -04 001 002 003 EYES (PUPIL SIZE, LACRIMATION, ETC.) FECES AND URINE (DECREASED, DARK, MUCOID, REDDISH, ETC.) GENERAL (SALIVATION, OBESE, NASAL DISCHARGE, ETC.) MOVEMENT/BEHAVIOR (INCOORD., MUSC. TONE, AGGRESSIVE, ETC.) RESPIRATION (DEEP, NOISY, SNEEZE, ETC.) SKIN, FUR AND MUCOUS MEMBRANES SENSORY RESPONSIVENESS(TOUCH, 'AUDITORY, NOCICEPTIVE, VISUAL) DISPOSITION (MISSING, MORIBUND, ETC.) SL 030930 P INDICATES THE OBSERVATION HAS PRESENT - INDICATES NO REMARKABLE OBSERVATIONS SL 030931 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Study ID: K-001716-091 Volume 2 of 3, Page: 94 Appendix E, continued: Functional Observational Battery INDIVIDUAL ANIMAL OBSERVATIONS ANIMAL ID: 9OA304O SEX: MALE SPECIES: RAT CONC; 2000 OBSERVATION(S) PPM DAT MONTH MONTH MONTH -04 001 002 003 EKES (PUPIL SIZE, LACKJMATION, ETC.) FECES AND URINE (DECREASED, DARK, MUCOID, REDDISH, ETC.) GENERAL (SALIVATION, OBESE, NASAL DISCHARGE, ETC.) MOVEMENT/BEHAVIOR (INCOORD., MUSC. TONE, AGGRESSIVE, ETC.) RESPIRATION (DEEP, NOISY, SNEEZE, ETC.I SKIN, FUR AND MUCOUS MEMBRANES SENSORY RESPONSIVENESS(TOUCH, 'AUDITORY, NOCICEPTIVE, VISUAL) DISPOSITION (MISSING, MORIBUND, ETC.) P INDICATES THE OBSERVATION HAS PRESENT - INDICATES NO REMARKABLE OBSERVATIONS 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Study ID: K-001716-091 Volume 2 of 3, Page: 95 Appendix E, continued: Functional Observational Battery INDIVIDUAL ANIMAL OBSERVATIONS ANIMAL ID: 90A3811 SEX: MALE SPECIES; RAT CONC: 2000 OBSERVATION(S) PPM DAT MONTH MONTH MONTH -04 001 002 003 EVES (PUPIL SIZE, LACRIMATION, ETC.) FECES AND URINE (DECREASED, DARK, MUCOID, REDDISH, ETC.I GENERAL (SALIVATION, OBESE, NASAL DISCHARGE, ETC.) MOVEMENT/BEHAVIOR (INCOORD., MUSC. TONE, AGGRESSIVE, ETC.) RESPIRATION (DEEP, NOISY, SNEEZE, ETC.) SKIN, FUR AND MUCOUS MEMBRANES SENSORY RESPONSIVENESS(TOUCH, 'AUDITORY, NOCICEPTIVE, VISUAL) DISPOSITION IMISSING, MORIBUND, ETC.) SL 030932 P INDICATES THE OBSERVATION HAS PRESENT - INDICATES NO REMARKABLE OBSERVATIONS SL 030933 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Study ID: K-001716-091 Volume 2 of 3, Page: 96 Appendix E, continued: Functional Observational Battery INDIVIDUAL ANIMAL OBSERVATIONS ANIMAL ID: 90A3842 SEX: MALE SPECIES: RAT CONC; 2000 OBSERVATION(SI PPM DAY MONTH MONTH MONTH -04 001 002 003 EKES (PUPIL SIZE, LACKIMATION, ETC.) FECES AND URINE (DECREASED, DARK, MUCOID, REDDISH, ETC.) GENERAL {SALIVATION, OBESE, NASAL DISCHARGE, ETC.) MOVEMENT/BEHAVIOR (INCOORD., MUSC. TONE, AGGRESSIVE, ETC.) RESPIRATION (DEEP, NOISY, SNEEZE, ETC.) SKIN, FUR AND MUCOUS MEMBRANES PAPULES,MUZZLE,RIGHT SENSORY RESPONSIVENESS(TOUCH, AUDITORY, NOCICEPTIVE, VISUAL) DISPOSITION (MISSING, MORIBUND, ETC.) P INDICATES THE OBSERVATION HAS PRESENT - INDICATES NO REMARKABLE OBSERVATIONS 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Study ID: K-001716-091 Volume 2 of 3, Page: 97 Appendix E, continued: Functional Observational Battery INDIVIDUAL ANIMAL OBSERVATIONS ANIMAL ID: 9OA3043 SEX: MALE SPECIES; RAT CONC: 2000 OBSERVATION(S) PPM INDIVIDUAL ANIMAL OBSERVATIONS DAY MONTH MONTH MONTH -04 001 002 003 EYES (PUPIL SIZE, LACRIMATION, ETC.) FECES AND URINE (DECREASED, DARK, MUCOID, REDDISH, ETC.) GENERAL (SALIVATION, OBESE, NASAL DISCHARGE, ETC.) MOVEMENT/BEHAVIOR (INCOORD., MUSC. TONE, AGGRESSIVE, ETC.) RESPIRATION (DEEP, NOISY, SNEEZE, ETC.) SKIN, FUR AND MUCOUS MEMBRANES SENSORY RESPONSIVENESS(TOUCH, 'AUDITORY, NOCICEPTIVE, VISUAL) DISPOSITION (MISSING, MORIBUND, ETC.) SL 030934 P INDICATES THE OBSERVATION HAS PRESENT - INDICATES NO REMARKABLE OBSERVATIONS SL 030935 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Study ID: K-001716-091 Volume 2 of 3, Page: 98 Appendix E, continued: Functional Observational Battery INDIVIDUAL ANIMAL OBSERVATIONS ANIMAL ID: 90A3844 SEX: MALE SPECIES: RAT CONC; 2000 OBSERVATION(S) PPM DAT MONTH MONTH MONTH -04 001 002 003 EVES (PUPIL SIZE, LACRIMATION, ETC.) EECES AND URINE (DECREASED, DARK, MUCOID, REDDISH, ETC.) GENERAL (SALIVATION, OBESE, NASAL DISCHARGE, ETC.) MOVEMENT/BEHAVIOR (INCOORD., MUSC. TONE, AGGRESSIVE, ETC.) RESPIRATION (OEEP, NOISY, SNEEZE, ETC.) SKIN, EUR AND MUCOUS MEMBRANES SENSORY RESPONSIVENESS(TOUCH, 'AUDITORY, NOCICEPTIVE, VISUAL) DISPOSITION (MISSING, MORIBUND, ETC.) P INDICATES THE OBSERVATION HAS PRESENT - INDICATES NO REMARKABLE OBSERVATIONS 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Study ID: K-001716-091 Volume 2 of 3, Page: 99 Appendix E, continued: Functional Observational Battery INDIVIDUAL ANIMAL OBSERVATIONS ANIMAL ID: 90A3B45 SEX: MALE SPECIES: RAT CONC: 2000 OBSERVATION |S) PPM DAY MONTH MONTH MONTH -04 001 002 003 EYES (PUPIL SI2E, LACHIMATION, ETC.) FECES AND URINE (DECREASED, DARK, MUCOID, REDDISH, ETC.) GENERAL (SALIVATION, OBESE, NASAL DISCHARGE, ETC.) MOVEMENT/BEHAVIOR (INCOORD., MUSC. TONE, AGGRESSIVE, ETC.) RESPIRATION (DEEP, NOISY, SNEEZE, ETC.) SKIN, FUR AND MUCOUS MEMBRANES SENSORY RESPONSIVENESS(TOUCH, 'AUDITORY, NOCICEPTIVE, VISUAL) DISPOSITION (MISSING, MORIBUND, ETC.) SL 030936 P INDICATES THE OBSERVATION WAS PRESENT - INDICATES NO REMARKABLE OBSERVATIONS SL 030937 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Study ID: K-001716-091 Volume 2 of 3, Page: 100 Appendix E, continued: Functional Observational Battery INDIVIDUAL ANIMAL OBSERVATIONS ANIMAL ID: 90A3846 SEX: MALE SPECIES: RAT CONC: 2000 OBSERVATION(S) PPM DAT MONTH MONTH MONTH -04 001 002 003 EYES (PUPIL SIZE, LACRIMATION, ETC.) FECES AND URINE (DECREASED, DARK, MUCOID, REDDISH, ETC.) GENERAL (SALIVATION, OBESE, NASAL DISCHARGE, ETC.) MOVEMENT/BEHAVIOR (INCOORD., MUSC. TONE, AGGRESSIVE, ETC.) RESPIRATION (DEEP, NOISY, SNEEZE, ETC.) SKIN, FUR AND MUCOUS MEMBRANES SENSORY RESPONSIVENESS(TOUCH,'AUDITORY, NOCICEPTIVE, VISUAL) DISPOSITION (MISSING, MORIBUND, ETC.) - * * * * * * P INDICATES THE OBSERVATION HAS PRESENT - INDICATES NO REMARKABLE OBSERVATIONS * INDICATES ANIMAL HAS NOT INCLUDED IN EXAM; ANIMAL STATUS: SP-DEATH DAY 059 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Study ID: K-001716-091 Volume 2 of 3, Page: 101 Appendix E, continued: Functional Observational Battery INDIVIDUAL ANIMAL OBSERVATIONS ANIMAL ID: 90A3847 SEX: MALE SPECIES: RAT CONC: 2000 OBSERVATION(S PPM DAT MONTH MONTH MONTH -04 001 002 003 EYES (PUPIL SIZE, LACRIMATION, ETC.) FECES AND URINE (DECREASED, DARK, MUCOID, REDDISH, ETC.) GENERAL (SALIVATION, OBESE, NASAL DISCHARGE, ETC.) MOVEMENT/BEHAVIOR (INCOORD., MUSC. TONE, AGGRESSIVE, ETC.) RESPIRATION (DEEP, NOISY, SNEEZE, ETC.) SKIN, FUR AND MUCOUS MEMBRANES SENSORY RESPONSIVENESS(TOUCH,'AUDITORY, NOCICEPTIVE, VISUAL) DISPOSITION (MISSING, MORIBUND, ETC.) SL 030938 P INDICATES THE OBSERVATION HAS PRESENT - INDICATES NO REMARKABLE OBSERVATIONS 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Study ID: K-001716-091 Volume 2 of 3, Page: 102 Appendix E, continued: Functional Observational Battery INDIVIDUAL ANIMAL OBSERVATIONS ANIMAL ID: 90A3848 SEX: FEMALE SPECIES: RAT conc: 0 CO OBSERVATION(SI f* EVES (PUPIL SIZE, LACRIMATION, ETC.) FECES AND URINE (DECREASED, DARK, MUCOID, REDDISH, ETC.I GENERAL (SALIVATION, OBESE, NASAL DISCHARGE, ETC.) MOVEMENT/BEHAVIOR (INCOORD., MUSC. TONE, AGGRESSIVE, ETC.) RESPIRATION (DEEP, NOISY, SNEEZE, ETC.> SKIN, FUR AND MUCOUS MEMBRANES SENSORY RESPONSIVENESS(TOUCH, 'AUDITORY, NOCICEPTIVE, VISUAL) DISPOSITION (MISSING, MORIBUND, ETC.) DAT MONTH MONTH MONTH -04 001 002 003 030939 P INDICATES THE OBSERVATION HAS PRESENT - INDICATES NO REMARKABLE OBSERVATIONS 030940 CO tr1 1,1,1 -T subchronic neurotoxicity The Dow Chemical Company Study ID: K-001716-091 Volume 2 of 3, Page: 103 Appendix E, continued: Functional Observational Battery INDIVIDUAL ANIMAL OBSERVATIONS ANIMAL ID: 90X3819 SEX: FEMALE SPECIES: RAT CONC: 0 OBSERVATION < S) PPM DAY MONTH MONTH MONTH -04 001 002 003 EYES (PUPIL SIZE, LACRIMATION, ETC.) FECES AND URINE (DECREASED, DARK, MUCOID, REDDISH, ETC.) GENERAL (SALIVATION, OBESE, NASAL DISCHARGE, ETC.) MOVEMENT/BEHAVIOR (INCOORD., MUSC. TONE, AGGRESSIVE, ETC.) RESPIRATION (DEEP, NOISY, SNEEZE, ETC.) SKIN, FUR AND MUCOUS MEMBRANES SENSORY RESPONSIVENESS(TOUCH,'AUDITORY, NOCICEPTIVE, VISUAL) DISPOSITION (MISSING, MORIBUND, ETC.) P INDICATES THE OBSERVATION HAS PRESENT - INDICATES NO REMARKABLE OBSERVATIONS 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Study ID: K401716491 Volume 2 of 3, Page: 104 Appendix E, continued: Functional Observational Battery INDIVIDUAL ANIMAL OBSERVATIONS ANIMAL ID: 90A3850 SEX: FEMALE SPECIES: CONC: RAT I0 OBSERVATION(S) PPM DAY MONTH MONTH MONTH -04 001 002 003 EYES (PUPIL SIZE, LACR1HATION, ETC.) FECES AND URINE (DECREASED, DARK, MUCOID, REDDISH, ETC.) GENERAL (SALIVATION, OBESE, NASAL DISCHARGE, ETC.) MOVEMENT/BEHAVIOR (INCOORD., MUSC. TONE, AGGRESSIVE, ETC.) RESPIRATION (DEEP, NOISY, SNEEZE, ETC.) SKIN, FUR AND MUCOUS MEMBRANES SENSORY RESPONSIVENESS(TOUCH, 'AUDITORY, NOCICEPTIVE, VISUAL) DISPOSITION (MISSING, MORIBUND, ETC.) SI* 0 3 0 9 4 1 P INDICATES THE OBSERVATION HAS PRESENT - INDICATES NO REMARKABLE OBSERVATIONS SL 030942 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Study ID: K-001716-091 Volume 2 of 3, Page: 105 Appendix E, continued: Functional Observational Battery INDIVIDUAL ANIMAL OBSERVATIONS ANIMAL ID: 90A38S1 SEX: FEMALE SPECIES: RAT conc: 0 OBSERVATION IS) PPM DAY MONTH MONTH MONTH -04 001 002 003 EYES (PUPIL SIZE, LACRIHATION, ETC.) FECES AND URINE (DECREASED, DARK, MUCOID, REDDISH, ETC.) GENERAL (SALIVATION, OBESE, NASAL DISCHARGE, ETC.) MOVEMENT/BEHAVIOR (INCOORD., KUSC. TONE, AGGRESSIVE, ETC.) RESPIRATION (DEEP, NOISY, SNEEZE, ETC.) SKIN, FUR AND MUCOUS MEMBRANES SENSORY RESPONSIVENESS(TOUCH, 'AUDITORY, NOCICEPTIVE, VISUAL) DISPOSITION (MISSING, MORIBUND, ETC.) P INDICATES THE OBSERVATION HAS PRESENT - INDICATES NO REMARKABLE OBSERVATIONS 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Study ID: K-001716-091 Volume 2 of 3, Page: 106 Appendix E, continued: Functional Observational Battery INDIVIDUAL ANIMAL OBSERVATIONS ANIMAL ID: 90A3852 SEX: FEMALE SPECIES: RAT CONC: 0 OBSERVATION(S) PPM DAY MONTH MONTH MONTH -04 001 002 003 EYES (PUPIL SIZE, LACRIMATION, ETC.) EXCESSIVE CHROMOOACYORRHEA, LEFT FECES AND URINE (DECREASED, DARK, MUCOID, REDDISH, ETC.) GENERAL (SALIVATION, OBESE, NASAL DISCHARGE, ETC.) MOVEMENT/BEHAVIOR (INCOORD., MUSC. TONE, AGGRESSIVE, ETC.) RESPIRATION (DEEP, NOISY, SNEEZE, ETC.) SKIN, FUR AND MUCOUS MEMBRANES SENSORY RESPONSIVENESS(TOUCH, AUDITORY, NOCICEPTIVE, VISUAL) AUDITORY RESPONSIVENESS - DECREASED DISPOSITION (MISSING, MORIBUND, ETC.) - P - p SL 030943 P INDICATES THE OBSERVATION HAS PRESENT - INDICATES NO REMARKABLE OBSERVATIONS 030944 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Study ID: K-001716-091 Volume 2 of 3, Page: 107 CO Appendix E, continued: Functional Observational Battery INDIVIDUAL ANIMAL OBSERVATIONS ANIMAL ID; 90A3853 SEX; FEMALE SPECIES: RAT cunc: u OBSERVATION(S) PPM DAY MONTH MONTH MONTH -04 001 002 003 EYES (PUPIL SIZE, LACRIMATION, ETC.) FECES AND URINE (DECREASED, DARK, MUCOID, REDDISH, ETC.) GENERAL (SALIVATION, OBESE, NASAL DISCHARGE, ETC.) MOVEMENT/BEHAVIOR (INCOORD., MUSC. TONE, AGGRESSIVE, ETC.) RESPIRATION (DEEP, NOISY, SNEEZE, ETC.) SKIN, FUR AND MUCOUS MEMBRANES SENSORY RESPONSIVENESS(TOUCH, 'AUDITORY, NOCICEPTIVE, VISUAL! DISPOSITION (MISSING, MORIBUND, ETC.) P INDICATES THE OBSERVATION NAS PRESENT - INDICATES NO REMARKABLE OBSERVATIONS 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Study ID: K-001716-091 Volume 2 of 3, Page: 108 Appendix E, continued: Functional Observational Battery INDIVIDUAL ANIMAL OBSERVATIONS ANIMAL ID: 90A3B54 SEX: FEMALE SPECIES: RAT CONC: 0 OBSERVATION(S| PPM DAY MONTH MONTH MONTH -04 001 002 003 EYES (PUPIL SIZE, LACRIMATION, ETC.) FECES AND URINE (DECREASED, DARK, MUCOID, REDDISH, ETC.) GENERAL (SALIVATION, OBESE, NASAL DISCHARGE, ETC.) MOVEMENT/BEHAVIOR (INCOORD., HUSC. TONE, AGCHESSIVE, ETC.) RESPIRATION (DEEP, NOISY, SNEEZE, ETC.) SKIN, FUR AND MUCOUS MEMBRANES SENSORY RESPONSIVENESS(TOUCH,'AUDITORY, NOCICEPTIVE, VISUAL) DISPOSITION (MISSING, MORIBUND, ETC.) O o JpLT P INDICATES THE OBSERVATION HAS PRESENT - INDICATES NO REMARKABLE OBSERVATIONS SL 030946 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Study ID: K-001716-091 Volume 2 of 3, Page: 109 Appendix E, continued: Functional Observational Battery INDIVIDUAL ANIMAL OBSERVATIONS ANIMAL ID: 90A38S5 SEX; FEMALE SPECIES: RAT CONC: 0 OBSERVATION IS) PPM DAT MONTH MONTH MONTH -04 001 002 003 EXES (PUPIL SIZE, LACRIMATION, ETC.) FECES AND URINE (DECREASED, DARK, MUCOID, REDDISH, ETC.) GENERAL (SALIVATION, OBESE, NASAL DISCHARGE, ETC.) MOVEMENT/BEHAVIOR (INCOORD., MUSC. TONE, AGGRESSIVE, ETC.) RESPIRATION (DEEP, NOISY, SNEEZE, ETC.) SKIN, FUR AND MUCOUS MEMBRANES SENSORY RESPONSIVENESSlTOUCH, `AUDITORY, NOCICEPTIVE, VISUAL) DISPOSITION (MISSING, MORIBUND, ETC.) P INDICATES THE OBSERVATION HAS PRESENT - INDICATES NO REMARKABLE OBSERVATIONS 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Study ID: K-001716-091 Volume 2 of 3, Page: 110 Appendix E, continued: Functional Observational Battery INDIVIDUAL ANIMAL OBSERVATIONS ANIMAL ID: 90A3856 SEX: FEMALE SPECIES: RAT CONC: 0 OBSERVATION(S) PPM DAK MONTH MONTH MONTH -04 001 002 003 EVES (PUPIL SIZE, LACRIMATION, ETC.) FECES AND URINE (DECREASED, DARK, MUCOID, REDDISH, ETC.) GENERAL (SALIVATION, OBESE, NASAL DISCHARGE, ETC.) MOVEMENT/BEHAVIOR (INCOORD., MUSC. TONE, AGGRESSIVE, ETC.) RESPIRATION (DEEP, NOISY, SNEEZE, ETC.) SKIN, FUR AND MUCOUS MEMBRANES CO SENSORY RESPONSIVENESS(TOUCH, 'AUDITORY, NOCICEPTIVE, VISUAL) ~ DISPOSITION (MISSING, MORIBUND, ETC.) 030947 P INDICATES THE OBSERVATION HAS PRESENT - INDICATES NO REMARKABLE OBSERVATIONS SL 030948 1,1,1 -T subchronic neurotoxicity The Dow Chemical Company Study ID: K-001716-091 Volume 2 of 3, Page: 111 Appendix E, continued: Functional Observational Battery INDIVIDUAL ANIMAL OBSERVATIONS ANIMAL ID: 90A3B57 SEX: FEMALE SPECIES: RAT CONC: 0 OBSERVATION(S) PPM DAY MONTH MONTH MONTH -0< 001 002 003 EYES IPUPIL SIZE, LACRIMATION, ETC.) FECES AND URINE (DECREASED, DARK, MUCOID, REDDISH, ETC.| GENERAL (SALIVATION, OBESE, NASAL DISCHARGE, ETC.) MOVEMENT/BEHAVIOR (INCOORD., MUSC. TONE, AGGRESSIVE, ETC.) RESPIRATION (DEEP, NOISY, SNEEZE, ETC.) SKIN, FUR AND MUCOUS MEMBRANES SENSORY RESPONSIVENESS(TOUCH, 'AUDITORY, NOCICEPTIVE, VISUAL) DISPOSITION (MISSING, MORIBUND, ETC.) P INDICATES THE OBSERVATION NAS PRESENT - INDICATES NO REMARKABLE OBSERVATIONS 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Study ID: K-001716-091 Volume 2 of 3, Page: 112 Appendix E, continued: Functional Observational Battery INDIVIDUAL ANIMAL OBSERVATIONS ANIMAL ID: 90A3B58 SEX: FEMALE SPECIES: RAT CONC: l0 OBSERVATION(S) PPM DAT MONTH MONTH MONTH -04 001 002 003 EYES (PUPIL SIZE, LACRIMATION, ETC.) FECES AND URINE (DECREASED, DARK, MUCOID, REDDISH, ETC.) GENERAL (SALIVATION, OBESE, NASAL DISCHARGE, ETC.) MOVEMENT/BEHAVIOR (INCOORD., MUSC. TONE, AGGRESSIVE, ETC.) RESPIRATION (DEEP, NOISY, SNEEZE, ETC.) SKIN, FUR AND MUCOUS MEMBRANES U) SENSORY RESPONSIVENESS(TOUCH, 'AUDITORY, NOCICEPTIVE, VISUAL) IT1 DISPOSITION (MISSING, MORIBUND, ETC.) 0309^9 P INDICATES THE OBSERVATION MAS PRESENT - INDICATES NO REMARKABLE OBSERVATIONS SL 030950 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Study ID: K-001716-091 Volume 2 of 3, Page: 113 Appendix E, continued: Functional Observational Battery INDIVIDUAL ANIMAL OBSERVATIONS ANIMAL ID: 90A3859 SEX: FEMALE SPECIES: RAT CONC; 0 OBSERVATION(S) PPH DAY MONTH MONTH MONTH -04 001 002 003 EYES (PUPIL SIZE, LACRINATION, ETC.) FECES AND URINE (DECREASED, DARK, MUCOID, REDDISH, ETC.) GENERAL (SALIVATION, OBESE, NASAL DISCHARGE, ETC.) MOVEMENT/BEHAVIOR (INCOORD., HUSC. TONE, AGGRESSIVE, ETC.) RESPIRATION (DEEP, NOISY, SNEEZE, ETC.) SKIN, FUR AND MUCOUS MEMBRANES SENSORY RESPONSIVENESS(TOUCH, 'AUDITORY, NOCICEPTIVE, VISUAL) DISPOSITION (MISSING, MORIBUND, ETC.) P INDICATES THE OBSERVATION HAS PRESENT - INDICATES NO REMARKABLE OBSERVATIONS 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Study ID: K-001716-091 Volume 2 of 3, Page: 114 Appendix E, continued: Functional Observational Battery INDIVIDUAL ANIMAL OBSERVATIONS ANIMAL ID: 90A3860 SEX: FEMALE SPEC IES: RAT CONC: i0 OBSERVATION(S) PPM DAT MONTH MONTH MONTH -04 001 002 003 EYES (PUPIL SIZE, LACRINATION, ETC.) FECES AND URINE (DECREASED, DARK, MUCOID, REDDISH, ETC.) GENERAL (SALIVATION, OBESE, NASAL DISCHARGE, ETC.) MOVEMENT/BEHAVIOR (INCOORD., MUSC. TONE, AGGRESSIVE, ETC.) RESPIRATION (DEEP, NOISY, SNEEZE, ETC.) SKIN, FUR AND MUCOUS MEMBRANES SENSORY RESPONSIVENESS(TOUCH, AUDITORY, NOCICEPTIVE, VISUAL) AUDITORY RESPONSIVENESS - DECREASED DISPOSITION (MISSING, MORIBUND, ETC.) -P SL 030951 P INDICATES THE OBSERVATION HAS PRESENT - INDICATES NO REMARKABLE OBSERVATIONS 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Study ID: K-001716-091 Volume 2 of 3, Page: 115 Appendix E, continued: Functional Observational Battery INDIVIDUAL ANIMAL OBSERVATIONS ANIMAL ID: 90A3S61 SEX: FEMALE SPECIES: RAT CONC: 0 OBSERVATION(S) PPM DAT MONTH MONTH MONTH -04 001 002 003 EYES {PUPIL SIZE, LACRIMATION, ETC.) FECES AND URINE (DECREASED, DARK, MUCOID, REDDISH, ETC.) GENERAL (SALIVATION, OBESE, NASAL DISCHARGE, ETC.) MOVEMENT/BEHAVIOR (INCOORD., MUSC. TONE, AGGRESSIVE, ETC.) RESPIRATION (DEEP, NOISY, SNEEZE, ETC.) SKIN, FUR AND MUCOUS MEMBRANES SENSORY RESPONSIVENESS(TOUCH, `AUDITORY, NOCICEPTIVE, VISUAL) DISPOSITION (MISSING, MORIBUND, ETC.) 030952 P INDICATES THE OBSERVATION HAS PRESENT - INDICATES NO REMARKABLE OBSERVATIONS 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Study ID: K-001716-091 Volume 2 of 3, Page: 116 Appendix E, continued: Functional Observational Battery INDIVIDUAL ANIMAL OBSERVATIONS ANIMAL ID: 90A3862 SEX: FEMALE SPECIES: RAT CONC: 200 OBSERVATION(S) PPM DAY MONTH MONTH MONTH -0( 001 002 003 EVES (PUPIL SIZE, LACRIMATION, ETC.) FECES AND URINE (DECREASED, DARK, KUCOI0, REDDISH, ETC.) GENERAL (SALIVATION, OBESE, NASAL DISCHARGE, ETC.) MOVEMENT/BEHAVIOR (INCOORD., MUSC. TONE, AGGRESSIVE, ETC.) RESPIRATION (DEEP, NOISY, SNEEZE, ETC.) SKIN, FUR AND MUCOUS MEMBRANES SENSORY RESPONSIVENESS(TOUCH,'AUDITORY, NOCICEPTIVE, VISUAL) DISPOSITION (MISSING, MORIBUND, ETC.) SL 030953 P INDICATES THE OBSERVATION HAS PRESENT - INDICATES NO REMARKABLE OBSERVATIONS SL 030954 1,1,I T subchronic neurotoxicity The Dow Chemical Company Study ID: K-001716-091 Volume 2 of 3, Page: 117 Appendix E, continued: Functional Observational Battery INDIVIDUAL ANIMAL OBSERVATIONS ANIMAL ID: 90A3863 SEX: FEMALE SPECIES: RAT CONC: 200 OBSERVATION IS) PPM EVES (PUPIL SIZE, LACRIMATION, ETC.) FECES AND URINE (DECREASED, DARK, MUCOID, REDDISH, ETC.) GENERAL (SALIVATION, OBESE, NASAL DISCHARGE, ETC.) MOVEMENT/BEHAVIOR (INCOORD., MUSC. TONE, AGGRESSIVE, ETC .) RESPIRATION (DEEP, NOISY, SNEEZE, ETC.) SKIN, FUR AND MUCOUS MEMBRANES SENSORY RESPONSIVENESS(TOUCH, AUDITORY, NOCICEPTIVE, VISUAL) DISPOSITION IMISSING, MORIBUND, ETC.) DAY MONTH MONTH MONTH -0* 001 002 003 ___ _ --- ----- - -- - --------- - P INDICATES THE OBSERVATION HAS PRESENT - INDICATES NO REMARKABLE OBSERVATIONS 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Study ID: K-001716-091 Volume 2 of 3, Page: 118 Appendix E, continued: Functional Observational Battery INDIVIDUAL ANIMAL OBSERVATIONS ANIMAL ID: 90A3864 SEX: FEMALE SPECIES; RAT CONC: 200 OBSERVATION(S) PPM DAT MONTH MONTH MONTH -04 001 002 003 EYES IPUPIL SIZE, LACRIMATION, ETC.) EECES AND URINE (DECREASED, DARK, MUCOID, REDDISH, ETC.) GENERAL (SALIVATION, OBESE, NASAL DISCHARGE, ETC.) MOVEMENT/BEHAVIOR (INCOORD., MUSC. TONE, AGGRESSIVE, ETC.) RESPIRATION (DEEP, NOISY, SNEEZE, ETC.) SKIN, FUR AND MUCOUS MEMBRANES SENSORY RESPONSIVENESS(TOUCH, 'AUDITORY, NOCICEPTIVE, VISUAL) DISPOSITION (MISSING, MORIBUND, ETC.) in c* 03095 P INDICATES THE OBSERVATION HAS PRESENT - INDICATES NO REMARKABLE OBSERVATIONS SL 030956 1,1,1 -T subchronic neurotoxicity The Dow Chemical Company Study ID: K-001716-091 Volume 2 of 3, Page: 119 yAppendix E, continued: Functional Observational Batter INDIVIDUAL ANIMAL OBSERVATIONS ANIMAL ID: 90A3865 SEX: FEMALE SPECIES: RAT CONC: 200 OBSERVATION|S) PPM DAY MONTH MONTH MONTH -04 001 002 003 EYES (PUPIL SIZE, LACKIMATION, ETC.) FECES AND URINE (DECREASED, DARK, MUCOID, REDDISH, ETC.) GENERAL (SALIVATION, OBESE, NASAL DISCHARGE, ETC.) MOVEMENT/BEHAVIOR (INCOORD., MUSC. TONE, AGGRESSIVE, ETC.) RESPIRATION (DEEP, NOISY, SNEEZE, ETC.) SKIN, FUR AND MUCOUS MEMBRANES SENSORY RESPONSIVENESS(TOUCH, 'AUDITORY, NOCICEPTIVE, VISUAL) DISPOSITION (MISSING, MORIBUND, ETC.) P INDICATES THE OBSERVATION HAS PRESENT - INDICATES NO REMARKABLE OBSERVATIONS 1,1,1-T subchronic neurotoxicity Hie Dow Chemical Company Study ID: K-001716491 Volume 2 of 3, Page: 120 Appendix E, continued: Functional Observational Battery INDIVIDUAL ANIMAL OBSERVATIONS ANIMAL ID: 90A3B66 SEX: FEMALE SPECIES: RAT CONC: 200 OBSERVATION(S| PPM DAY MONTH MONTH MONTH -04 001 002 003 EYES (PUPIL SIZE, LACR1MATION, ETC.} FECES AND URINE (DECREASED, DARK, MUCOID, REDDISH, ETC.) GENERAL (SALIVATION, OBESE, NASAL DISCHARGE, ETC.) MOVEMENT/BEHAVIOR (INCOORD., MUSC. TONE, AGGRESSIVE, ETC.) RESPIRATION (DEEP, NOISY, SNEEZE, ETC.) SKIN, FUR AND MUCOUS MEMBRANES SENSORY RESPONSIVENESS(TOUCH, AUDITORY, NOCICEPTIVE, VISUAL) DISPOSITION IMISSING, MORIBUND, ETC.) * ---* * - * * * * C/1 V* 030957 P INDICATES THE OBSERVATION HAS PRESENT - INDICATES NO REMARKABLE OBSERVATIONS * INDICATES ANIMAL HAS NOT INCLUDED IN EXAM; ANIMAL STATUS: SP-DEATH DAY 066 SL 030958 1,1,I T subchronic neurotoxicity The Dow Chemical Company Study ID: K-001716-091 Volume 2 of 3, Page: 121 Appendix E, continued: Functional Observational Battery INDIVIDUAL ANIMAL OBSERVATIONS ANIMAL ID: 90A3867 SEX: PEMALE SPECIES: RAT CONC: 200 OBSERVATION(S) PPM DAT MONTH MONTH MONTH -04 001 002 003 ETES (PUPIL SIZE, LACRIMATION, ETC.I EXCESSIVE CHROMODACYORRHEA, RIGHT FECES AND URINE (DECREASED, DARK, MUCOID, REDDISH, ETC.) GENERAL (SALIVATION, OBESE, NASAL DISCHARGE, ETC.) MOVEMENT/BEHAVIOR (INCOORD., MUSC. TONE, AGGRESSIVE, ETC.) RESPIRATION (DEEP, NOISY, SNEEZE, ETC.) SKIN, PUR AND MUCOUS MEMBRANE!) SENSORY RESPONSIVENESS(TOUCH, AUDITORY, NOCICEPTIVE, VISUAL) DISPOSITION (MISSING, MORIBUND, ETC.) - p P INDICATES THE OBSERVATION MAS PRESENT - INDICATES NO REMARKABLE OBSERVATIONS 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Study ID: K-001716-091 Volume 2 of 3, Page: 122 Appendix E, continued: Functional Observational Battery INDIVIDUAL ANIMAL OBSERVATIONS ANIMAL ID: 90A3868 SEX: FEMALE SPECIES: RAT CONC: 200 OBSERVATION(S) PPM DAT MONTH MONTH MONTH -04 001 002 003 EYES (PUPIL SIZE, LACRIMATION, ETC.) FECES AND URINE (DECREASED, DARK, MUCOID, REDOISH, ETC.) GENERAL (SALIVATION, OBESE, NASAL DISCHARGE, ETC.) MOVEMENT/BEHAVIOR (INCOORD., MUSC. TONE, AGGRESSIVE, ETC.) RESPIRATION (DEEP, NOISY, SNEEZE, ETC.) SKIN, FUR AND MUCOUS MEMBRANES SENSORY RESPONSIVENESS(TOUCH, AUDITORY, NOCICEPTIVE, VISUAL) DISPOSITION (MISSING, MORIBUND, ETC.) SL 030959 P INDICATES THE OBSERVATION HAS PRESENT - INDICATES NO REMARKABLE OBSERVATIONS SL 030960 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Study ID: K-001716491 Volume 2 of 3, Page: 123 Appendix E, continued: Functional Observational Battery INDIVIDUAL ANIMAL OBSERVATIONS ANIMAL ID: 90A3869 SEX: FEMALE SPECIES: RAT CONC: 200 OBSERVAT ION(SI PPM DAT MONTH MONTH MONTH -04 001 002 003 EYES (PUPIL SIZE, LACR1MATION, ETC.) FECES AND URINE (DECREASED, DARK, MUCOID, REDDISH, ETC.) GENERAL (SALIVATION, OBESE, NASAL DISCHARGE, ETC.) MOVEMENT/BEHAVIOR (INCOORD., MUSC. TONE, AGGRESSIVE, ETC.) RESPIRATION (DEEP, NOISY, SNEEZE, ETC.) SKIN, FUR AND MUCOUS MEMBRANES SENSORY RESPONSIVENESS(TOUCH,'AUDITORY, NOCICEPTIVE, VISUAL) DISPOSITION (MISSING, MORIBUND, ETC.) P INDICATES THE OBSERVATION WAS PRESENT - INDICATES NO REMARKABLE OBSERVATIONS 1,1,1 -T subchronic neurotoxicity The Dow Chemical Company Study ID: K-001716-091 Volume 2 of 3, Page; 124 Appendix E, continued: Functional Observational Battery INDIVIDUAL ANIMAL OBSERVATIONS ANIMAL ID: 90A3870 SEX: FEMALE SPECIES: RAT CONC; 200 OBSERVATION(S) DAY MONTH MONTH MONTH -04 001 002 003 EYES (PUPIL SIZE, LACRIMATION, ETC.) FECES AND URINE (DECREASED, DARK, MUCOID, REDDISH, ETC.) GENERAL (SALIVATION, OBESE, NASAL DISCHARGE, ETC.) MOVEMENT/BEHAVIOR (INCOORD., MUSC. TONE, AGGRESSIVE, ETC.) RESPIRATION (DEEP, NOISY, SNEEZE, ETC.) SKIN, FUR AND MUCOUS MEMBRANES SENSORY RESPONSIVENESS(TOUCH, `AUDITORY, NOCICEPTIVE, VISUAL) DISPOSITION (MISSING, MORIBUND, ETC.) SL 030961 P INDICATES THE OBSERVATION HAS PRESENT - INDICATES NO REMARKABLE OBSERVATIONS SL 030962 1,1,1-T subchronk neurotoxicity The Dow Chemical Company Study ID: K-001716-091 Volume 2 of 3, Page: 125 Appendix E, continued: Functional Observational Battery INDIVIDUAL ANIMAL OBSERVATIONS ANIMAL ID: 90A3871 SEX: FEMALE SPECIES: RAT CONC: 200 OBSERVATION(S) PPM DAT MONTH MONTH MONTH -04 001 002 003 EKES (PUPIL SIZE, LACRIMATION, ETC.) EXCESSIVE CHROMODACYORRHEA,BILATERAL EECES AND URINE (DECREASED, DARK, MUCOID, REDDISH, ETC.) GENERAL (SALIVATION, OBESE, NASAL DISCHARGE, ETC.) MOVEMENT/BEHAVIOR (INCOORD., MUSC. TONE, AGGRESSIVE, ETC.) RESPIRATION (DEEP, NOISY, SNEEZE, ETC.) SKIN, PUR AND MUCOUS MEMBRANES SENSORY RESPONSIVENESS(TOUCH, AUDITORY, NOCICEPTIVE, VISUAL) DISPOSITION (MISSING, MORIBUND, ETC.) p ---- P INDICATES THE OBSERVATION HAS PRESENT - INDICATES NO REMARKABLE OBSERVATIONS 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Study ID: K-001716-091 Volume 2 of 3, Page: 126 Appendix E, continued: Functional Observational Battery INDIVIDUAL ANIMAL OBSERVATIONS ANIMAL ID: 90A3872 SEX: FEMALE SPECIES: RAT CONC: 200 OBSERVATION(S) PPM EYES (PUPIL SIZE, LACRIHATION, ETC.) FECES AND URINE (DECREASED, DARK, MUCOID, REDDISH, ETC.) GENERAL (SALIVATION, OBESE, NASAL DISCHARGE, ETC.) MOVEMENT/BEHAVIOR (INCOORD., MUSC. TONE, AGGRESSIVE, ETC.) RESPIRATION (DEEP, NOISY, SNEEZE, ETC.) SKIN, FUR AND MUCOUS MEMBRANES SENSORY RESPONSIVENESS(TOUCH, 'AUDITORY, NOCICEPTIVE, VISUAL) DISPOSITION (MISSING, MORIBUND, ETC.) DAY MONTH MONTH MONTH -04 001 002 003 ___ - --- - - -- - --- --- - --- SL 030963 P INDICATES THE OBSERVATION HAS PRESENT - INDICATES NO REMARKABLE OBSERVATIONS SL 030964 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Study ID: K-001716-091 Volume 2 of 3, Page: 127 Appendix E, continued: Functional Observational Battery FUNCTIONAL OBSERVATIONAL BATTERY INDIVIDUAL ANIMAL OBSERVATIONS ANIMAL ID: 90A3673 SEX; FEMALE SPECIES: RAT CONC: 200 OBSERVATION(S) PPM DAY MONTH MONTH MONTH -09 001 002 003 EYES (PUPIL SIZE, LACRIMATION, ETC.) FECES AND URINE (DECREASED, DARK, MUCOID, REDDISH, ETC.) GENERAL (SALIVATION, OBESE, NASAL DISCHARGE, ETC.) MOVEMENT/BEHAVIOR (INCOORD., HUSC. TONE, AGGRESSIVE, ETC.) RESPIRATION (DEEP, NOISY, SNEEZE, ETC.) SKIN, FUR AND MUCOUS MEMBRANES SENSORY RESPONSIVENESS(TOUCH, AUDITORY, NOCICEPTIVE, VISUAL) DISPOSITION (MISSING, MORIBUND, ETC.) P INDICATES THE OBSERVATION HAS PRESENT - INDICATES NO REMARKABLE OBSERVATIONS 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Study ID: K-001716-091 Volume 2 of 3, Page: 128 Appendix E, continued: Functional Observational Battery INDIVIDUAL ANIMAL OBSERVATIONS ANIMAL ID: 90A3874 SEX: FEMALE SPECIES: RAT CONC: 200 OBSERVATION(S) PPM DAY MONTH MONTH MONTH -04 001 002 003 EYES (PUPIL SIZE, LACRIHATION, ETC.) FECES AND URINE (DECREASED, DARK, MUCOID, REDDISH, ETC.) GENERAL (SALIVATION, OBESE, NASAL DISCHARGE, ETC.) MOVEMENT/BEHAVIOR (INCOORO., MUSC. TONE, AGGRESSIVE, ETC.) RESPIRATION (DEEP, NOISY, SNEEZE, ETC.) UT SKIN, FUR AND MUCOUS MEMBRANES r* SENSORY RESPONSIVENESS(TOUCH, AUDITORY, NOCICEPTIVE, VISUAL) DISPOSITION (MISSING, MORIBUND, ETC.) 030965 P INDICATES THE OBSERVATION HAS PRESENT - INDICATES NO REMARKABLE OBSERVATIONS SL 030966 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Study ID: K-001716-091 Volume 2 of 3, Page: 129 Appendix E, continued: Functional Observational Battery INDIVIDUAL ANIMAL OBSERVATIONS ANIMAL ID: 90A3875 SEX: FEMALE SPECIES: RAT CONC: 200 OBSERVATION(S) PPM DAY MONTH MONTH MONTH -04 001 002 003 EYES (PUPIL SIZE, LACRIMATION, ETC.) FECES AND URINE (DECREASED, DARK, MUCOID, REDDISH, ETC.) GENERAL (SALIVATION, OBESE, NASAL DISCHARGE, ETC.) MOVEMENT/BEHAVIOR (INCOORD., MUSC. TONE, AGGRESSIVE, ETC.) RESPIRATION (DEEP, NOISY, SNEEZE, ETC.) SKIN, FUR AND MUCOUS MEMBRANES SENSORY RESPONSIVENESS(TOUCH, 'AUDITORY, NOCICEPTIVE, VISUAL) DISPOSITION (MISSING, MORIBUND, ETC.) P INDICATES THE OBSERVATION HAS PRESENT - INDICATES NO REMARKABLE OBSERVATIONS SL 030967 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Study ID: K-001716-091 Volume 2 of 3, Page: 130 Appendix E, continued: Functional Observational Battery INDIVIDUAL ANIMAL OBSERVATIONS ANIMAL ID: 90A3876 SEX: FEMALE SPECIES: RAT CONC: 6 30 OBSERVATION(S) PPM DAY MONTH MONTH MONTH -04 001 002 003 EYES (PUPIL SIZE, LACRIMATION, ETC.I FECES AND URINE (DECREASED, DARK, MUCOID, REDDISH, ETC.) GENERAL (SALIVATION, OBESE, NASAL DISCHARGE, ETC.) MOVEMENT/BEHAVIOR (INCOORD., MUSC. TONE, AGGRESSIVE, ETC.) RESPIRATION (DEEP, NOISY, SNEEZE, ETC.) SKIN, TUR AND MUCOUS MEMBRANES SENSORY RESPONSIVENESS(TOUCH, 'AUDITORY, NOCICEPTIVE, VISUAL) DISPOSITION IMISSING, MORIBUND, ETC.) P INDICATES THE OBSERVATION HAS PRESENT - INDICATES NO REMARKABLE OBSERVATIONS 896oeo Co 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Study ID: K-001716-091 Volume 2 of 3, Page: 131 Appendix E, continued: Functional Observational Battery INDIVIDUAL ANIMAL OBSERVATIONS ANIMAL ID: 90A3873 SEX: FEMALE SPECIES: RAT CONC: 630 OBSERVATION(S) PPM DAT MONTH MONTH MONTH -04 001 002 003 EYES (PUPIL SIZE, LACRIMATION, ETC.) f'ECES AND URINE (DECREASED, DARK, MUCOID, REDDISH, ETC. I GENERAL (SALIVATION, OBESE, NASAL DISCHARGE, ETC.) MOVEMENT/BEHAVIOR (INCOORD., MUSC. TONE, AGGRESSIVE, ETC.) RESPIRATION (DEEP, NOISY, SNEEZE, ETC.) SKIN, PUR AND MUCOUS MEMBRANES SENSORY RESPONSIVENESS(TOUCH, 'AUDITORY, NOCICEPTIVE, VISUAL) AUDITORY RESPONSIVENESS - DECREASED DISPOSITION (MISSING, MORIBUND, ETC.) - - p P INDICATES THE OBSERVATION HAS PRESENT - INDICATES NO REMARKABLE OBSERVATIONS 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Study ID: KTXH716-091 Volume 2 of 3, Page: 132 Appendix E, continued: Functional Observational Battery NDIV1DUAL ANIMAL OBSERVATIONS ANIMAL ID: 90A3B18 SEX: FEMALE SPECIES: RAT CONC: 630 OBSERVATION(S) PPM DAY MONTH MONTH MONTH -0* 001 002 003 EYES (PUPIL SIZE, LACRIHATION, ETC.) FECES AND URINE (DECREASED, DARK, MUCOID, REDDISH, ETC.) GENERAL (SALIVATION, OBESE, NASAL DISCHARGE, ETC.) MOVEMENT/BEHAVIOR (INCOORD., MUSC. TONE, AGGRESSIVE, ETC.) RESPIRATION (DEEP, NOISY, SNEEZE, ETC.) SKIN, FUR AND MUCOUS MEMBRANES SENSORY RESPONSIVENESS(TOUCH, 'AUDITORY, NOCICEPTIVE, VISUAL) DISPOSITION (MISSING, MORIBUND, ETC.) 030969 CO t* P INDICATES THE OBSERVATION NAS PRESENT - INDICATES NO REMARKABLE OBSERVATIONS c/i t* 030970 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Study ID: K-001716-091 Volume 2 of 3, Page: 133 Appendix E, continued: Functional Observational Battery INDIVIDUAL ANIMAL OBSERVATIONS ANIMAL ID; 90A3879 SEX: FEMALE SPEC IES: RAT CONC; 630 OBSERVATION(S) PPM EYES (PUPIL SIZE, LACRIMATION, ETC.) FECES AND URINE (DECREASED, DARK, MUCOID, REDDISH, ETC.) GENERAL (SALIVATION, OBESE, NASAL DISCHARGE, ETC.) MOVEMENT/BEHAVIOR (INCOORD., MUSC. TONE, AGGRESSIVE, ETC.I AGGRESSIVE RESPIRATION (DEEP, NOISY, SNEEZE, ETC.I SKIN, fUR AND MUCOUS MEMBRANES SENSORY RESPONSIVENESS(TOUCH, AUDITORY, NOCICEPTIVE, VISUAL) DISPOSITION (MISSING, MORIBUND, ETC.) DAY MONTH MONTH MONTH -0( 001 002 003 __ --- - --- - ? - P INDICATES THE OBSERVATION HAS PRESENT - INDICATES NO REMARKABLE OBSERVATIONS 1,1,1 -T subchronic neurotoxicity The Dow Chemical Company Study ID: K-001716-091 Volume 2 of 3, Page: 134 Appendix E, continued: Functional Observational Battery INDIVIDUAL ANIMAL OBSERVATIONS ANIMAL ID: 90A3B80 SEX: FEMALE SPECIES: RAT CONC: 630 OBSERVATION(S) PPM DAT MONTH MONTH MONTH -04 001 002 003 EYES (PUPIL SIZE, LACRIMAT10N, ETC.) FECES AND URINE (DECREASED, DARK, MUCOID, REDDISH, ETC.) GENERAL (SALIVATION, OBESE, NASAL DISCHARGE, ETC.) MOVEMENT/BEHAVIOR (INCOORD., MUSC. TONE, AGGRESSIVE, ETC.) RESPIRATION (DEEP, NOISY, SNEEZE, ETC.) SKIN, FUR AND MUCOUS MEMBRANES SENSORY RESPONSIVENESS(TOUCH,'AUDITORY, NOCICEPTIVE, VISUAL) DISPOSITION (MISSING, MORIBUND, ETC.) P INDICATES THE OBSERVATION MAS PRESENT - INDICATES NO REMARKABLE OBSERVATIONS 6 0 t 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Study ID: K-001716-091 Volume 2 ol 3, Page: 135 Appendix E, continued: Functional Observational Battery INDIVIDUAL ANIMAL OBSERVATIONS ANIMAL ID: 90A3881 SEX: FEMALE SPECIES: RAT CONC: 630 PPM t>3 OBSERVATION (S) DAY MONTH MONTH MONTH -0$ 001 OOP 003 EYES (PUPIL SIZE, LACRIMATION, ETC.) FECES AND URINE (DECREASED, DARK, MUCOID, REDDISH, ETC.) GENERAL (SALIVATION, OBESE, NASAL DISCHARGE, ETC.) MOVEMENT/BEHAVIOR (INCOORD., MUSC. TONE, AGGRESSIVE, ETC.) RESPIRATION (DEEP, NOISY, SNEEZE, ETC.) SKIN, FUR AND MUCOUS MEMBRANES SENSORY RESPONSIVENESS(TOUCH, 'AUDITORY, NOCICEPTIVE, VISUAL) DISPOSITION (MISSING, MORIBUND, ETC.) P INDICATES THE OBSERVATION HAS PRESENT - INDICATES NO REMARKABLE OBSERVATIONS 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Study ID: K-001716-091 Volume 2 of 3, Page: 136 Appendix E, continued: Functional Observational Battery INDIVIDUAL ANIMAL OBSERVATIONS ANIMAL ID: 90A3681 SEX: FEMALE SPECIES: RAT CONC: 630 OBSERVATION IS) PPM DAY MONTH MONTH MONTH -04 001 002 003 EYES (PUPIL SIZE, LACRIMATION, ETC.) FECES AND URINE (DECREASED, DARK, MUCOID, REDDISH, ETC.) GENERAL (SALIVATION, OBESE, NASAL DISCHARGE, ETC.) MOVEMENT/BEHAVIOR (INCOORD., MUSC. TONE, AGGRESSIVE, ETC.) RESPIRATION (DEEP, NOISY, SNEEZE, ETC.) SKIN, FUR AND MUCOUS MEMBRANES SENSORY RESPONSIVENESS(TOUCH, 'AUDITORY, NOCICEPTIVE, VISUAL) DISPOSITION (MISSING, MORIBUND, ETC.) INDICATES THE OBSERVATION NAS PRESENT ^ INDICATES NO REMARKABLE OBSERVATIONS SL 030974 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Study ID: K-001716-091 Volume 2 of 3, Page: 137 Appendix E, continued: Functional Observational Battery INDIVIDUAL ANIMAL OBSERVATIONS ANIMAL ID: 90A3683 SEX: FEMALE SPECIES: RAT CONC: 630 OBSERVATION(S> PPM EKES (PUPIL SIZE, LACRIMATION, ETC.) FECES AND URINE (DECREASED, DARK, MUCOID, REDDISH, ETC.) GENERAL (SALIVATION, OBESE, NASAL DISCHARGE, ETC.) MOVEMENT/BEHAVIOR (INCOORD., MUSC. TONE, AGGRESSIVE, ETC.) RESPIRATION (DEEP, NOISY, SNEEZE, ETC.) SKIN, FUR AND MUCOUS MEMBRANES SENSORY RESPONSIVENESS(TOUCH, 'AUDITORY, NOCICEPTIVE, VISUAL) DISPOSITION (MISSING, MORIBUND, ETC.) DAY MONTH MONTH MONTH -04 001 002 003 ___ ------- - -- - ------ --- - --- P INDICATES THE OBSERVATION HAS PRESENT - INDICATES NO REMARKABLE OBSERVATIONS 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Study ID: K-001716-091 Volume 2 of 3, Page: 138 Appendix E, continued: Functional Observational Battery INDIVIDUAL ANIMAL OBSERVATIONS ANIMAL ID: 90A3884 SEX: FEMALE SPECIES: RAT CONC: 630 OBSERVATION(S) PPM DAY MONTH MONTH MONTH -04 001 002 003 EYES (PUPIL SIZE, LACRIMATION, ETC.) PECES AND URINE (DECREASED, DARK, MUCOID, REDDISH, ETC.) GENERAL (SALIVATION, OBESE, NASAL DISCHARGE, ETC.) MOVEMENT/BEHAVIOR (INCOORD., MUSC. TONE, AGGRESSIVE, ETC.) RESPIRATION (DEEP, NOISY, SNEEZE, ETC.) SKIN, FUR AND MUCOUS MEMBRANES SENSORY RESPONSIVENESS(TOUCH, 'AUDITORY, NOCICEPTIVE, VISUAL) DISPOSITION (MISSING, MORIBUND, ETC.) SL 030975 P INDICATES THE OBSERVATION HAS PRESENT - INDICATES NO REMARKABLE OBSERVATIONS SX- 0 3 0 9 7 6 1(1,1-T subchronic neurotoxicity The Dow Chemical Company Study ID: K-001716-091 Volume 2 of 3, Page: 139 Appendix E, continued: Functional Observational Battery INDIVIDUAL ANIMAL OBSERVATIONS ANIMAL ID: 90A3885 SEX: FEMALE SPECIES: RAT CONC: 630 OBSERVATION(S) PPM DAT MONTH MONTH MONTH -04 001 002 003 EYES (PUPIL SIZE, LACRIMATION, ETC.) FECES AND URINE (DECREASED, DARK, MUCOID, REDDISH, ETC.) GENERAL (SALIVATION, OBESE, NASAL DISCHARGE, ETC.) MOVEMENT/BEHAVIOR (INCOORD., MUSC. TONE, AGGRESSIVE, ETC.) RESPIRATION (DEEP, HOIST, SNEEZE, ETC.) SKIN, FUR AND MUCOUS MEMBRANES SENSOR* RESPONSIVENESS(TOUCH,'AUDITOR*, NOCICEPTIVE, VISUAL) DISPOSITION (MISSING, MORIBUND, ETC.) P INDICATES THE OBSERVATION MAS PRESENT - INDICATES NO REMARKABLE OBSERVATIONS I 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Study ID: K-001716-091 Volume 2 of 3, Page: 140 Appendix E, continued: Functional Observational Battery INDIVIDUAL ANIMAL OBSERVATIONS ANIMAL ID: 90A3886 SEX: FEMALE SPECIES: RAT CONC: 630 OBSERVATION(S) PPM DAY MONTH MONTH MONTH -04 001 002 003 EYES (PUPIL SIZE, LACRIMATION, ETC.) FECES AND URINE (DECREASED, DARK, MUCOID, REDDISH, ETC.) GENERAL (SALIVATION, OBESE, NASAL DISCHARGE, ETC.) MOVEMENT/BEHAVIOR (INCOORD., HUSC. TONE, AGGRESSIVE, ETC.) RESPIRATION (DEEP, NOISY, SNEEZE, ETC.) SKIN, FUR AND MUCOUS MEMBRANES SENSORY RESPONSIVENESS(TOUCH,'AUDITORY, NOCICEPTIVE, VISUAL) DISPOSITION (MISSING, MORIBUND, ETC.) SL 030977 P INDICATES THE OBSERVATION HAS PRESENT - INDICATES NO REMARKABLE OBSERVATIONS SL 030978 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Study ID: K-001716-091 Volume 2 of 3, Page: 141 Appendix E, continued: Functional Observational Battery INDIVIDUAL ANIMAL OBSERVATIONS ANIMAL ID: 90A3887 SEX: FEMALE SPECIES; RAT CONC: 630 OBSERVATION(S) PPM DAT MONTH MONTH MONTH -01 001 002 003 EVES (PUPIL SIZE, LACRIMATION, ETC.) FECES AND URINE (DECREASED, DARK, MUCOID, REDDISH, ETC.) GENERAL (SALIVATION, OBESE, NASAL DISCHARGE, ETC.) MOVEMENT/BEHAVIOR lINCOORD., MUSC. TONE, AGGRESSIVE, ETC.) RESPIRATION (DEEP, NOISY, SNEEZE, ETC.) SKIN, FUR AND MUCOUS MEMBRANES SENSORY RESPONSIVENESS(TOUCH,'AUDITORY, NOCICEPTIVE, VISUAL) DISPOSITION (MISSING, MORIBUND, ETC.) P INDICATES THE OBSERVATION HAS PRESENT - INDICATES NO REMARKABLE OBSERVATIONS 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Study ID; K-00171&091 Volume 2 of 3, Page: 142 Appendix G, continued; Functional Observational Battery INDIVIDUAL ANIMAL OBSERVATIONS ANIMAL ID: 90A38B8 SEX: FEMALE SPECIES: RAT CONC: 630 OBSERVATION(S) PPM DAY MONTH MONTH MONTH -04 001 002 003 EYES (PUPIL SIZE, LACRIMATION, ETC.) FECES AND URINE (DECREASED, DARK, MUCOID, REDDISH, ETC.I GENERAL (SALIVATION, OBESE, NASAL DISCHARGE, ETC.) MOVEMENT/BEflAVIOR (INCOORD., MUSC. TONE, AGGRESSIVE, ETC.) RESPIRATION (DEEP, NOISY, SNEEZE, ETC.) SKIN, FUR AND MUCOUS MEMBRANES SENSORY RESPONSIVENESS(TOUCH,'AUDITORY, NOCICEPTIVE, VISUAL) DISPOSITION (MISSING, MORIBUND, ETC.) P INDICATES THE OBSERVATION NAS PRESENT - INDICATES NO REMARKABLE OBSERVATIONS SL 030980 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Study ID: K-001716-091 Volume 2 of 3, Page: 143 Appendix E, continued: Functional Observational Battery INDIVIDUAL ANIMAL OBSERVATIONS ANIMAL ID: 90A3889 SEX; FEMALE SPECIES: RAT CONC: 630 OBSERVATION(S) PPM DAY MONTH MONTH MONTH -04 001 002 003 EYES (PUPIL SIZE, LACRINATION, ETC.) EXCESSIVE CHROMODACYORRHEA, LEFT FECES AND URINE (DECREASED, DARK, MUCOID, REDDISH, ETC.) GENERAL (SALIVATION, OBESE, NASAL DISCHARGE, ETC.) MOVEMENT/BEHAVIOR (INCOORD., MUSC. TONE, AGGRESSIVE, ETC.) RESPIRATION (DEEP, NOISY, SNEEZE, ETC.) SKIN, FUR AND MUCOUS MEMBRANES SENSORY RESPONSIVENESS(TOUCH, AUDITORY, NOCICEPTIVE, VISUAL) DISPOSITION (MISSING, MORIBUND, ETC.) p ---- P INDICATES THE OBSERVATION HAS PRESENT - INDICATES NO REMARKABLE OBSERVATIONS 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Study ID: K-001716-091 Volume 2 of 3, Page: 144 Appendix E, continued: Functional Observational Battery INDIVIDUAL ANIMAL OBSERVATIONS ANIMAL ID: 90A3B90 SEX: FEMALE SPECIES: RAT CONC: 2000 OBSERVATION(S) PPM DAT MONTH MONTH MONTH -04 001 002 003 EYES (PUPIL SIZE, LACRIMATION, ETC.) FECES AND URINE (DECREASED, DARK, MDCOIO, REDDISH, ETC.) GENERAL (SALIVATION, OBESE, NASAL DISCHARGE, ETC.) MOVEMENT/BEHAVIOR (INCOORO., MUSC. TONE, AGGRESSIVE, ETC.) RESPIRATION (DEEP, NOISY, SNEEZE, ETC.) SKIN, FUR AND MUCOUS MEMBRANES SENSORY RESPONSIVENESS(TOUCH, 'AUDITORY, NOCICEPTIVE, VISUAL) DISPOSITION (MISSING, MORIBUND, ETC.) [/) 030981 P INDICATES THE OBSERVATION HAS PRESENT - INDICATES NO REMARKABLE OBSERVATIONS SL 030982 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Study ID: K-001716-091 Volume 2 of 3, Page: 145 Appendix E, continued: Functional Observational Battery INDIVIDUAL ANIMAL OBSERVATIONS ANIMAL ID: 90A3891 SX: FEMALE SPECIES: RAT CONC: 2000 OBSERVATION(S) PPM DAY MONTH MONTH MONTH -04 001 002 003 EYES (PUPIL SIZE, LACRIMATION, ETC.) FECES AND URINE (DECREASED, DARK, MUCOID, REDDISH, ETC.) GENERAL (SALIVATION, OBESE, NASAL DISCHARGE, ETC.) MOVEMENT/BEHAVIOR (INCOORD., MUSC. TONE, AGGRESSIVE, ETC.) RESPIRATION (DEEP, NOISY, SNEEZE, ETC.) SKIN, FUR AND MUCOUS MEMBRANES SENSORY RESPONSIVENESS(TOUCH,'AUDITORY, NOCICEPTIVE, VISUAL) DISPOSITION (MISSING, MORIBUND, ETC.) P INDICATES THE OBSERVATION HAS PRESENT - INDICATES NO REMARKABLE OBSERVATIONS 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Study ID: K-001716-091 Volume 2 of 3, Page: 146 Appendix E, continued: Functional Observational Battery INDIVIDUAL ANIMAL OBSERVATIONS ANIMAL ID: 90A3B92 SEX: FEMALE SPECIES: RAT cone; 2000 OBSERVATION(S) PPM DAT MONTH MONTH MONTH -04 001 002 003 EYES (PUPIL SI2E, LACR1MATION, ETC.) FECES AND URINE (DECREASED, DARK, MUCOID, REDDISH, ETC.) GENERAL (SALIVATION, OBESE, NASAL DISCHARGE, ETC.) MOVEMENT/BEHAVIOR (INCOORD., MUSC. TONE, AGGRESSIVE, ETC.) RESPIRATION (DEEP, NOISY, SNEEZE, ETC.) SKIN, FUR AND MUCOUS MEMBRANES SENSORY RESPONSIVENESS(TOUCH, 'AUDITORY, NOCICEPTIVE, VISUAL) DISPOSITION (MISSING, MORIBUND, ETC.) P INDICATES THE OBSERVATION MAS PRESENT - INDICATES NO REMARKABLE OBSERVATIONS i 9 tf 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Study ID: K-001716-091 Volume 2 of 3, Page: 147 Appendix E, continued: Functional Observational Battery INDIVIDUAL ANIMAL OBSERVATIONS ANIMAL ID: 90A3B93 SEX: FEMALE SPECIES: RAT CONC: 2000 OBSERVATION(S) PPM DAY MONTH MONTH MONTH -04 001 002 003 EYES (PUPIL SIZE, LACRIKATION, ETC.) FECES AND URINE (DECREASED, DARK, MUCOID, REDDISH, ETC.| GENERAL (SALIVATION, OBESE, NASAL DISCHARGE, ETC.) MOVEMENT/BEHAVIOR (INCOORD., MUSC. TONE, AGGRESSIVE, ETC.) RESPIRATION (DEEP, NOISY, SNEEZE, ETC.) SKIN, FUR AND MUCOUS MEMBRANES SENSORY RESPONSIVENESS(TOUCH, 'AUDITORY, NOCICEPTIVE, VISUAL) DISPOSITION (MISSING, MORIBUND, ETC.) P INDICATES THE OBSERVATION HAS PRESENT - INDICATES NO REMARKABLE OBSERVATIONS 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Study ID: K-001716-091 Volume 2 of 3, Page: 148 Appendix E, continued: Functional Observational Battery INDIVIDUAL ANIMAL OBSERVATIONS ANIMAL ID: 90A3894 SEX: FEMALE SPECIES: RAT CONC: 2000 OBSERVATION IS) DAK MONTH MONTH MONTH -04 001 002 003 EYES (PUPIL SIZE, LACRIKATIOK, ETC.) FECES AND URINE (DECREASED, DARK, MUCOID, REDDISH, ETC.) GENERAL (SALIVATION, OBESE, NASAL DISCHARGE, ETC.) MOVEMENT/BEHAVIOR (INCOORD., HUSC. TONE, AGGRESSIVE, ETC.) RESPIRATION (DEEP, NOISY, SNEEZE, ETC.) SKIN, FUR AND MUCOUS MEMBRANES SENSORY RESPONSIVENESS(TOUCH,'AUDITORY, NOCICEPTIVE, VISUAL) DISPOSITION IMISSING, MORIBUND, ETC.) SL 030985 P INDICATES THE OBSERVATION HAS PRESENT - INDICATES NO REMARKABLE OBSERVATIONS * INDICATES ANIMAL HAS NOT INCLUDED IN EXAM; ANIMAL STATUS: SP-DEATH DAY 066 S l* 030986 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Study ID: K-001716-091 Volume 2 of 3, Page: 149 Appendix E, continued: Functional Observational Battery INDIVIDUAL ANIMAL OBSERVATIONS ANIMAL ID 90A3895 SEX: FEMALE SPECIES: RAT CONC: 2000 OBSERVATION(S> PPM DAT MONTH MONTH MONTH -0* 001 002 003 EYES (PUPIL SIZE, LACKIMATION, ETC.) FECES AND URINE (DECREASED, DARK, MUCOID, REDDISH, ETC.) GENERAL (SALIVATION, OBESE, NASAL DISCHARGE, ETC.) MOVEMENT/BEHAVIOR (INCOORD., MUSC. TONE, AGGRESSIVE, ETC.) RESPIRATION (DEEP, NOISY, SNEEZE, ETC.> SKIN, FUR AND MUCOUS MEMBRANES SENSORY RESPONSIVENESS(TOUCH, 'AUDITORY, NOCICEPTIVE, VISUAL) DISPOSITION (MISSING, MORIBUND, ETC.) P INDICATES THE OBSERVATION HAS PRESENT - INDICATES NO REMARKABLE OBSERVATIONS 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Study ID: K-001716-09I Volume 2 of 3, Page: 150 Appendix E, continued: Functional Observational Battery INDIVIDUAL ANIMAL OBSERVATIONS ANIMAL ID: 90A3896 SEX: FEMALE SPECIES RAT conc: 2000 OBSERVATION(S) PPM DAY MONTH MONTH MONTH -04 001 002 003 EYES (PUPIL SI2E, LACRIMATION, ETC.) FECES AND URINE (DECREASED, DARK, MUCOID, REDDISH, ETC.) GENERAL (SALIVATION, OBESE, NASAL DISCHARGE, ETC.) MOVEMENT/BEHAVIOR (INCOORD., MUSC. TONE, AGGRESSIVE, ETC.) RESPIRATION (DEEP, NOISY, SNEEZE, ETC.) SKIN, FUR AND MUCOUS MEMBRANES SENSORY RESPONSIVENESS(TOUCH,`AUDITORY, NOCICEPTIVE, VISUALI DISPOSITION (MISSING, MORIBUND, ETC.) tn c* P INDICATES THE OBSERVATION HAS PRESENT - INDICATES NO REMARKABLE OBSERVATIONS * INDICATES ANIMAL HAS NOT INCLUDED IN EXAM; ANIMAL STATUS: SP-DEATH DAY 0S7 SL 030988 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Study ID: K-001716-091 Volume 2 of 3, Page: 151 Appendix E, continued: Functional Observational Battery INDIVIDUAL ANIMAL OBSERVATIONS ANIMAL ID; 90A3B97 SEX; FEMALE SPECIES: RAT CONC: 2000 OBSERVATION(S) PPM DAY MONTH MONTH MONTH -04 001 002 003 EYES (PUPIL SIZE, LACRIMATION, ETC.) FECES AND URINE (DECREASED, DARK, MUCOID, REDDISH, ETC.) GENERAL (SALIVATION, OBESE, NASAL DISCHARGE, ETC.) MOVEMENT/BEHAVIOR (INCOORD., MUSC. TONE, AGGRESSIVE, ETC.) RESPIRATION (DEEP, NOISY, SNEEZE, ETC.) SKIN, FUR AND MUCOUS MEMBRANES SENSORY RESPONSIVENESS(TOUCH,'AUDITORY, NOCICEPTIVE, VISUAL) DISPOSITION (MISSING, MORIBUND, ETC.) P INDICATES THE OBSERVATION HAS PRESENT - INDICATES NO REMARKABLE OBSERVATIONS 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Study ID: K-001716-091 Volume 2 o/ 3, Page: 152 Appendix E, continued: Functional Observational Battery INDIVIDUAL ANIMAL OBSERVATIONS ANIMAL ID: 90A389B SEX: FEMALE SPECIES RAT conc: 2000 OBSERVATION IS) PPM DAT MONTH MONTH MONTH -04 001 002 003 EYES (PUPIL SIZE, LACRIMATION, ETC.} FECES AND URINE (DECREASED, DARK, MUCOID, REDDISH, ETC.) GENERAL (SALIVATION, OBESE, NASAL DISCHARGE, ETC.) MOVEMENT/BEHAVIOR (INCOORO., MUSC. TONE, AGGRESSIVE, ETC.) RESPIRATION (DEEP, NOISY, SNEEZE, ETC.) SKIN, FUR AND MUCOUS MEMBRANES SENSORY RESPONSIVENESS(TOUCH, 'AUDITORY, NOCICEPTIVE, VISUAL) DISPOSITION (MISSING, MORIBUND, ETC.) SL 030989 P INDICATES THE OBSERVATION HAS PRESENT - INDICATES NO REMARKABLE OBSERVATIONS 030990 c/i t~> 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Study ID: K-001716-091 Volume 2 of 3, Page: 153 Appendix E, continued: Functional Observational Battery INDIVIDUAL ANIMAL OBSERVATIONS ANIMAL ID: 90A3899 SEX: FEMALE SPECIES: RAT CONC: 2000 OBSERVATION(S) PPM DAY MONTH MONTH MONTH -04 001 002 003 EYES (PUPIL SIZE, LACRIMATION, ETC.! FECES AND URINE (DECREASED, DARX, MUCOID, REDDISH, ETC.! GENERAL (SALIVATION, OBESE, NASAL DISCHARGE, ETC.) MOVEMENT/BEHAVIOR (INCOORD., MUSC. TONE, AGGRESSIVE, ETC.) RESPIRATION (DEEP, NOISY, SNEEZE, ETC.) SKIN, FUR AND MUCOUS MEMBRANES SENSORY RESPONSIVENESS(TOUCH, 'AUDITORY, NOCICEPTIVE, VISUAL) DISPOSITION (MISSING, MORIBUND, ETC.) P INDICATES THE OBSERVATION HAS PRESENT - INDICATES NO REMARKABLE OBSERVATIONS 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Study ID: K-001716-091 Volume 2 of 3, Page: 154 Appendix E, continued: Functional Observational Battery INDIVIDUAL ANIMAL OBSERVATIONS ANIMAL ID: 90A3900 SEX: FEMALE SPECIES: RAT CONC: 2000 OBSERVATION IS) PPM DAY MONTH MONTH MONTH -04 001 002 003 EYES (PUPIL SIZE, LACKIMATION, ETC.) FECES AND URINE (DECREASED, DARK, MUCOID, REDDISH, ETC.) GENERAL (SALIVATION, OBESE, NASAL DISCHARGE, ETC.) MOVEMENT/BEHAVIOR (INCOORD., MUSC. TONE, AGGRESSIVE, ETC.) RESPIRATION (DEEP, NOISY, SNEEZE, ETC.) SKIN, FUR AND MUCOUS MEMBRANES SENSORY RESPONSIVENESS(TOUCH, AUDITORY, NOCICEPTIVE, VISUAL) DISPOSITION (MISSING, MORI MIND, ETC.) P INDICATES THE OBSERVATION HAS PRESENT - INDICATES NO REMARKABLE OBSERVATIONS SL 030992 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Study ID: K-001716491 Volume 2 of 3, Page: 155 Appendix E, continued: Functional Observational Battery INDIVIDUAL ANIMAL OBSERVATIONS ANIMAL ID: 90A3901 SEX: FEMALE SPECIES: RAT CONC: 2000 OBSERVAT ION(S) PPM DAY MONTH MONTH MONTH -09 001 002 003 EYES (PUPIL SIZE, LACRIMATION, ETC.) FECES AND URINE (DECREASED, DARK, MUCOID, REDDISH, ETC.) GENERAL (SALIVATION, OBESE, NASAL DISCHARGE, ETC.) MOVEMENT/BEHAVIOR (INCOORD., MUSC. TONE, AGGRESSIVE, ETC.) RESPIRATION (DEEP, NOISY, SNEEZE, ETC.) SKIN, FUR AND MUCOUS MEMBRANES SENSORY RESPONSIVENESS(TOUCH, AUDITORY, NOCICEPTIVE, VISUAL) DISPOSITION (MISSING, MORIBUND, ETC.) P INDICATES THE OBSERVATION NAS PRESENT - INDICATES NO REMARKABLE OBSERVATIONS 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Study ID: K-001716491 Volume 2 of 3, Page: 156 Appendix E, continued: Functional Observational Battery INDIVIDUAL ANIMAL OBSERVATIONS ANIMAL ID; 90A3902 SEX: FEMALE SPECIES: RAT CONC: 2000 OBSERVATION(S) PPM DAY MONTH MONTH MONTH -0 001 002 003 EVES (PUPIL SIZE, LACKIHATION, ETC.) FECES AND URINE (DECREASED, DARK, HUCOID, REDDISH, ETC.) GENERAL (SALIVATION, OBESE, NASAL DISCHARGE, ETC.) MOVEMENT/BEHAVIOR (INCOORD., MUSC. TONE, AGGRESSIVE, ETC.) RESPIRATION (DEEP, HOIST, SNEEZE, ETC.) SKIN, FUR AND MUCOUS MEMBRANES SENSORY RESPONSIVENESS(TOUCH, AUDITORY, NOCICEPTIVE, VISUAL) DISPOSITION (MISSING, MORIBUND, ETC.) 030993 r1 P INDICATES THE OBSERVATION HAS PRESENT - INDICATES NO REMARKABLE OBSERVATIONS i SL 030994 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Study ID: K-001716-091 Volume 2 of 3, Page: 157 Appendix E, continued: Functional Observational Battery INDIVIDUAL. ANIMAL OBSERVATIONS ANIMAL ID: 90A3903 SEX: FEMALE SPECIES: RAT CONC: 2000 OBSERVATION(S) PPM DAT MONTH MONTH MONTH -04 001 002 003 EYES (PUPIL SIZE, LACRIMATION, ETC.) FECES AND URINE (DECREASED, DARK, MUCOID, REDDISH, ETC.) GENERAL (SALIVATION, OBESE, NASAL DISCHARGE, ETC.) MOVEMENT/BEHAVIOR (INCOORD., MUSC. TONE, AGGRESSIVE, ETC.) RESPIRATION (DEEP, NOISY, SNEEZE, ETC.) SKIN, FUR AND MUCOUS MEMBRANES SENSORY RESPONSIVENESS(TOUCH,'AUDITORY, NOCICEPTIVE, VISUAL) DISPOSITION (MISSING, MORIBUND, ETC.) P INDICATES THE OBSERVATION HAS PRESENT - INDICATES NO REMARKABLE OBSERVATIONS 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 2 of 3, Page: 158 Appendix F: Grip Performance Data Hindlimb IQ 90A3792 90A3793 90A3794 90A3795 90A3796 90A3797 90A3798 90A3799 90A3800 90A3801 90A3802 90A3803 90A3804 90A3805 90A3806 90A3807 90A3808 90A3809 90A3810 90A3811 90A3812 90A3813 90A3814 90A3815 90A3816 90A3817 90A3818 90A3819 90A3820 90A3821 90A3822 90A3823 90A3824 Sex Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Group A A A A A A A B B B B B B B A A A A A A A B B B B B B B A A A A A Cone 0 ppm 0 ppm 0 ppm 0 ppm 0 ppm 0 ppm 0 ppm 0 ppm 0 ppm 0 ppm 0 ppm 0 ppm 0 ppm 0 ppm 200 ppm 200 ppm 200 ppm 200 ppm 200 ppm 200 ppm 200 ppm 200 ppm 200 ppm 200 ppm 200 ppm 200 ppm 200 ppm 200 ppm 630 ppm 630 ppm 630 ppm 630 ppm 630 ppm PreExp 570 535 390 570 430 620 560 520 425 500 380 530 515 660 500 470 510 540 610 565 540 485 500 565 660 545 385 355 550 620 530 600 450 Month 1 Month 2 Month 3 570 550 500 700 560 710 550 710 a 550 440 650 450 600 610 650 680 720 560 560 730 580 570 590 475 635 610 510 630 b 560 635 585 580 635 650 450 630 650 720 595 750 455 405 555 390 540 610 630 620 850 485 640 670 625 700 670 580 480 730 530 720 810 550 435 480 490 635 450 640 630 700 660 805 b 620 715 680 630 680 440 575 600 580 550 730 750 640 540 810 600 600 780 610 750 660 490 590 660 aAnimal died prior to testing; ^Missing data. sl 030993 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 2 of 3, Page: 159 Appendix F, continued: Grip Performance Data Hindlimb m 90A3825 90A3826 90A3827 90A3828 90A3829 90A3830 90A3831 90A3832 90A3833 90A3834 90A3835 90A3836 90A3837 90A3838 90A3839 90A3840 90A3841 90A3842 90A3843 90A3844 90A3845 90A3846 90A3847 90A3848 90A3849 90A3850 90A3851 90A3852 90A3853 90A3854 90A3855 90A3856 90A3857 Sex Group Cone Male A 630 ppm Male A 630 ppm Male B 630 ppm Male B 630 ppm Male B 630 ppm Male B 630 ppm Male B 630 ppm Male B 630 ppm Male B 630 ppm Male A 2000 ppm Male A 2000 ppm Male A 2000 ppm Male A 2000 ppm Male A 2000 ppm Male A 2000 ppm Male A 2000 ppm Male B 2000 ppm Male B 2000 ppm Male B 2000 ppm Male B 2000 ppm Male B 2000 ppm Male B 2000 ppm Male B 2000 ppm Female A 0 ppm Female A 0 ppm Female A 0 ppm Female A 0 ppm Female A 0 ppm Female A 0 ppm Female A 0 ppm Female B 0 ppm Female B 0 ppm Female B 0 ppm PreExp 615 530 445 650 700 505 545 430 635 600 695 410 525 450 600 540 670 595 610 510 400 615 550 450 250 350 340 290 . 310 470 345 400 320 Month 1 Month 2 Month 3 580 660 590 595 680 595 500 560 a 520 740 750 670 530 800 710 705 650 640 620 660 470 430 540 610 735 830 520 610 790 510 450 670 630 685 630 590 720 840 510 640 590 560 745 600 550 610 640 625 635 670 620 820 760 530 650 750 450 580 630 540 645 450 600 675 a 530 680 490 485 490 535 280 410 430 400 410 420 270 430 570 370 390 370 455 375 560 400 410 530 330 510 370 500 600 600 400 245 510 aAnimal died prior to testing. 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 2 of 3, Page: 160 Appendix F, continued: Grip Performance Data Hindlimb ID 90A3858 90A38S9 90A3860 90A3861 90A3862 90A3863 90A3864 90A3865 90A3866 90A3867 90A3868 90A3869 90A3870 90A3871 90A3872 90A3873 90A3874 90A3875 90A3876 90A3877 90A3878 90A3879 90A3880 90A3881 90A3882 90A3883 90A3884 90A3885 90A3886 90A3887 90A3888 90A3889 90A3890 Sex Group Cone Female B 0 ppm Female B 0 ppm Female B 0 ppm Female B 0 ppm Female A 200 ppm Female A 200 ppm Female A 200 ppm Female A 200 ppm Female A 200 ppm Female A 200 ppm Female A 200 ppm Female B 200 ppm Female B 200 ppm Female B 200 ppm Female B 200 ppm Female B 200 ppm Female B 200 ppm Female B 200 ppm Female A 630 ppm Female A 630 ppm Female A 630 ppm Female A 630 ppm Female A 630 ppm Female A 630 ppm Female A 630 ppm Female B 630 ppm Female B 630 ppm Female B 630 ppm Female B 630 ppm Female B 630 ppm Female B 630 ppm Female B 630 ppm Female A 2000 ppm PreExp 340 315 240 350 420 410 440 215 380 315 300 390 315 250 280 300 335 460 305 290 230 265 300 400 300 240 325 300 285 420 320 190 320 Month 1 Month 2 Month 3 460 380 a 325 395 400 445 385 560 365 440 380 365 440 570 470 470 630 480 550 500 360 235 425 320 380 a 385 450 440 400 260 400 360 310 495 265 305 430 385 420 a 435 445 550 450 470 300 330 275 345 410 320 410 400 450 530 400 270 480 300 355 380 310 315 430 330 450 440 395 500 500 330 260 485 340 300 395 365 480 310 410 450 430 310 260 360 340 420 530 340 500 455 290 330 540 490 305 565 aAnimal died prior to testing. 030997 1,1/1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 2 of 3, Page: 161 Appendix F, continued: Grip Performance Data Hindlimb ID 90A3891 90A3892 90A3893 90A3894 90A3895 90A3896 90A3897 90A3898 90A3899 90A3900 90A3901 90A3902 90A3903 Sex Group Cone Female A 2000 ppm Female A 2000 ppm Female A 2000 ppm Female A 2000 ppm Female A 2000 ppm Female A 2000 ppm Female B 2000 ppm Female B 2000 ppm Female B 2000 ppm Female B 2000 ppm Female B 2000 ppm Female B 2000 ppm Female B 2000 ppm PreExp 300 410 345 400 450 330 215 355 370 340 315 380 375 Month 1 Month 2 Month 3 420 335 450 265 320 480 335 345 600 265 220 a 375 410 460 430 380 a 250 325 590 360 360 400 350 330 320 370 310 370 380 250 510 315 400 420 335 400 355 aAnimal died prior to testing. Sl 030998 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 2 of 3, Page: 162 Appendix F, continued: Grip Performance Data Foreiimb ID 90A3792 90A3793 90A3794 90A3795 90A3796 90A3797 90A3798 90A3799 90A3800 90A3801 90A3802 90A3803 90A3804 90A3805 90A3806 90A3807 90A3808 90A3809 90A3810 90A3811 90A3812 90A3813 90A3814 90A3815 90A3816 90A3817 90A3818 90A3819 90A3820 90A3821 90A3822 90A3823 90A3824 Sex Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Group A A A A A A A B B B B B B B A A A A A A A B B B B B B B A A A A A Cone 0 ppm 0 ppm 0 ppm 0 ppm 0 ppm 0 ppm 0 ppm 0 ppm 0 ppm 0 ppm 0 ppm 0 ppm 0 ppm 0 ppm 200 ppm 200 ppm 200 ppm 200 ppm 200 ppm 200 ppm 200 ppm 200 ppm 200 ppm 200 ppm 200 ppm 200 ppm 200 ppm 200 ppm 630 ppm 630 ppm 630 ppm 630 ppm 630 ppm PreExp 845 810 670 750 840 670 620 610 650 750 580 620 840 945 810 900 695 800 760 795 900 760 710 950 870 830 600 710 830 480 795 885 710 Month 1 Month 2 Month 3 640 480 540 700 690 720 880 660 a 770 665 630 690 670 770 620 735 730 600 655 710 745 845 600 630 740 680 730 730 b 790 665 660 660 700 650 710 715 640 905 745 870 730 735 555 630 715 565 700 540 750 680 675 730 555 595 780 700 650 580 720 645 650 670 670 760 795 840 710 720 600 710 715 670 a 770 770 810 680 810 545 710 655 550 910 635 725 605 630 580 670 670 630 700 790 800 715 695 730 aAnimal died prior to testing; ^Missing data. 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 2 of 3, Page: 163 Appendix F, continued: Grip Performance Data Forelimb 22 90A3825 90A3826 90A3827 90A3828 90A3829 90A3830 90A3831 90A3832 90A3833 90A3834 90A3835 90A3836 90A3837 90A3838 90A3839 90A3840 90A3841 90A3842 90A3843 90A3844 90A3845 90A3846 90A3847 90A3848 90A3849 90A3850 90A3851 90A38S2 90A3853 90A3854 90A3855 90A3856 90A38S7 Sex Group Cone Male A 630 ppm Male A 630 ppm Male B 630 ppm Male B 630 ppm Male B 630 ppm Male B 630 ppm Male B 630 ppm Male B 630 ppm Male B 630 ppm Male A 2000 ppm Male A 2000 ppm Male A 2000 ppm Male A 2000 ppm Male A 2000 ppm Male A 2000 ppm Male A 2000 ppm Male B 2000 ppm Male B 2000 ppm Male B 2000 ppm Male B 2000 ppm Male B 2000 ppm Male B 2000 ppm Male B 2000 ppm Female A 0 ppm Female A 0 ppm Female A 0 ppm Female A 0 ppm Female A 0 ppm Female A 0 ppm Female A 0 ppm Female B 0 ppm Female B 0 ppm Female B 0 ppm PreExD 775 655 650 950 850 695 850 645 990 870 600 850 700 650 910 750 650 725 620 700 775 790 670 755 550 640 600 495 635 590 535 630 520 Month 1 Month 2 Month 3 710 820 630 820 810 590 815 660 a 880 835 865 590 630 490 900 690 740 680 710 860 670 650 580 835 670 640 685 570 530 670 820 570 670 600 510 655 700 590 770 560 860 760 720 400 865 810 625 670 530 560 610 650 780 620 495 560 745 780 930 640 700 600 525 720 a 675 730 540 960 530 700 480 590 620 590 610 585 590 550 580 485 580 560 780 350 570 485 500 540 610 530 580 680 550 640 570 580 580 aAnimal died prior to testing. Si 31000 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 2 of 3, Page: 164 Appendix F, continued: Grip Performance Data Forelimb ID 90A3858 90A3861 90A3862 90A3863 90A3864 90A386S 90A3866 90A3867 90A3868 90A3869 90A3870 90A3871 90A3872 90A3873 90A3874 90A3875 90A3876 90A3877 90A3878 90A3879 90A3880 90A3881 90A3882 90A3883 90A3884 90A3885 90A3886 90A3887 90A3888 90A3889 90A3890 90A3891 90A3892 Sex Group Cone Female B 0 ppm Female B 0 ppm Female A 200 ppm Female A 200 ppm Female A 200 ppm Female A 200 ppm Female A 200 ppm Female A 200 ppm Female A 200 ppm Female B 200 ppm Female B 200 ppm Female B 200 ppm Female B 200 ppm Female B 200 ppm Female B 200 ppm Female B 200 ppm Female A 630 ppm Female A 630 ppm Female A 630 ppm Female A 630 ppm Female A 630 ppm Female A 630 ppm Female A 630 ppm Female B 630 ppm Female B 630 ppm Female B 630 ppm Female B 630 ppm Female B 630 ppm Female B 630 ppm Female B 630 ppm Female A 2000 ppm Female A 2000 ppm Female A 2000 ppm PreExD 555 610 660 610 660 660 700 530 700 565 630 515 670 620 465 680 620 580 690 500 640 760 620 635 640 525 600 565 . 530 530 590 515 660 Month 1 Month 2 Month 3 580 460 a 600 650 490 590 620 560 620 480 730 725 545 625 620 540 450 730 530 a 715 485 550 695 655 550 540 550 575 620 470 460 620 565 a 655 590 445 610 530 530 580 485 420 720 550 600 485 545 640 680 550 590 505 440 460 500 520 510 560 610 530 650 550 745 520 565 545 750 650 465 580 550 380 635 535 460 550 560 435 740 400 460 650 560 550 545 510 430 510 525 620 565 590 570 615 580 490 aAnimal died prior to testing. SL 031001 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 2 of 3, Page: 165 Appendix F, continued: Grip Performance Data Foreiimb ID 90A3893 90A3894 90A3895 90A3896 90A3897 90A3898 90A3899 90A3900 90A3901 90A3902 90A3903 Sex GrouD Cone Female A 2000 ppm Female A 2000 ppm Female A 2000 ppm Female A 2000 ppm Female B 2000 ppm Female B 2000 ppm Female B 2000 ppm Female B 2000 ppm Female B 2000 ppm Female B 2000 ppm Female B 2000 ppm PreExp 610 560 560 740 400 505 485 560 585 610 520 Month 1 Month 2 Month 3 675 600 560 590 425 a 550 545 390 345 475 a 585 580 440 625 465 330 520 400 380 655 530 495 610 300 320 760 530 515 590 465 500 aAnimal died prior to testing. SL 031002 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 2 of 3, Page: 166 ID 90A3792 90A3795 90A3797 90A3799 90A3800 90A3802 90A3803 90A3806 90A3808 90A3809 90A3812 90A3815 90A3817 90A3818 90A3820 90A3823 90A3824 90A3828 90A3829 90A3832 90A3834 90A3835 90A3840 90A3842 90A3843 90A3847 90A3848 90A3849 90A3850 90A3851 90A3853 90A3860 90A3861 90A3863 90A3867 90A3868 Appendix F, continued: Grip Performance Data Post Exposure Body Wt, Hindlimb and Forelimb Sex Group Male A Male A Male A Male B Male B Male B Male B Male A Male A Male A Male A Male B Male B Male B Male A Male A Male A Male B Male B Male B Male A Male A Male A Male B Male B Male B Female A Female A Female A Female A Female A Female B Female B Female A Female A Female A Cone 0 ppm 0 ppm 0 ppm 0 ppm 0 ppm 0 ppm 0 ppm 200 ppm 200 ppm 200 ppm 200 ppm 200 ppm 200 ppm 200 ppm 630 ppm 630 ppm 630 ppm 630 ppm 630 ppm 630 ppm 2000 ppm 2000 ppm 2000 ppm 2000 ppm 2000 ppm 2000 ppm 0 ppm 0 ppm 0 ppm 0 ppm 0 ppm 0 ppm 0 ppm 200 ppm 200 ppm 200 ppm Body Wt Hindlimb 344.6 735 349.7 1040 342.5 1020 396.0 1150 353.4 740 336.9 1215 374.6 740 411.5 785 367.5 865 351.1 940 388.2 940 354.9 1110 331.6 975 347.3 1065 362.6 880 378.2 1015 373.6 970 397.5 1405 371.0 835 372.7 700 368.6 740 380.0 1005 291.3 780 390.0 680 315.9 940 362.1 745 194.1 1025 204.5 585 . 207.4 655 196.4 625 196.1 830 189.4 965 184.9 740 198.5 800 197.9 650 185.4 575 Forelimb 615 830 750 565 545 800 605 555 495 680 670 735 895 650 640 600 490 745 765 370 700 560 415 675 650 560 400 460 340 330 590 505 360 475 310 400 03*00^ 1,1,1 -T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 2 of 3, Page: 167 ID 90A3870 90A3872 90A3873 90A3874 90A3876 90A3878 90A3879 90A3881 90A3884 90A3887 90A3888 90A3889 90A3890 90A3895 90A3898 90A3899 90A3900 90A3902 90A3903 Appendix F, continued: Grip Performance Data Post Exposure Body Wt., Hindlimb and Forelimb Sex Group Female B Female B Female B Female B Female A Female A Female A Female A Female B Female B Female B Female B Female A Female A Female B Female B Female B Female B Female B Cone 200 ppm 200 ppm 200 ppm 200 ppm 630 ppm 630 ppm 630 ppm 630 ppm 630 ppm 630 ppm 630 ppm 630 ppm 2000 ppm 2000 ppm 2000 ppm 2000 ppm 2000 ppm 2000 ppm 2000 ppm BodvWt Hindlimb 193.9 640 188.7 790 198.8 720 189.8 350 206.7 705 190.1 520 205.1 450 195.3 705 200.6 600 196.1 425 205.9 710 196.9 550 198.2 750 199.4 600 192.6 540 194.7 340 188.7 510 190.5 570 195.9 565 Forelimb 400 455 310 385 555 310 270 355 245 400 650 310 450 520 425 400 400 360 295 Sl 031004 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 2 of 3, Page: 168 Appendix G: Temperature Data ID 90A3792 90A3793 90A3795 90A3796 90A3797 90A3798 90A3799 90A3800 90A3802 90A3803 90A3804 90A3805 90A3806 90A3807 90A3808 90A3809 90A3810 90A3811 90A3813 90A3814 90A3815 90A3817 90A3818 90A3819 90A3820 90A3821 90A3822 90A3823 90A3824 90A3825 90A3828 90A3829 SEX Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male GROUP CONC A 0 ppm A 0 ppm A 0 ppm A 0 ppm A 0 ppm A 0 ppm B 0 ppm B 0 ppm B 0 ppm B 0 ppm B 0 ppm B 0 ppm A 200 ppm A 200 ppm A 200 ppm A 200 ppm A 200 ppm A 200 ppm B 200 ppm B 200 ppm B 200 ppm B 200 ppm B 200 ppm B 200 ppm A 630 ppm A 630 ppm A 630 ppm A 630 ppm A 630 ppm A 630 ppm B 630 ppm B 630 ppm FEPT 38.7 37.5 36.5 38.3 37.4 37.6 38.4 37.2 38.0 37.4 37.0 37.5 37.6 38.0 37.5 38.4 37.5 38.4 38.8 38.5 37.0 37.5 39.0 38.6 37.9 38.0 37.0 38.0 37.1 38.5 38.0 37.9 ABRCT 38.4 37.2 36.7 38.8 37.6 37.9 38.0 37.8 37.9 37.5 37.6 38.2 37.3 38.0 37.9 38.6 37.6 38.5 38.7 38.4 37.8 37.3 38.8 38.5 37.7 37.8 38.0 38.4 37.7 38.4 38.3 37.6 ABRTT 38.4 37.2 36.7 38.7 37.6 37.9 38.0 37.8 37.9 37.5 37.4 38.2 37.3 38.0 37.9 38.5 37.6 38.6 38.7 38.4 37.9 37.3 38.8 38.5 37.7 37.7 38.1 38.4 37.9 38.4 38.3 37.6 SEPT 38.5 37.7 36.6 38.7 37.6 37.9 37.8 37.8 37.8 37.2 37.4 38.1 37.2 38.0 37.9 385 37.6 38.5 38.6 38.3 37.9 37.4 38.9 38.5 37.6 37.7 38.2 38.4 37.9 385 38.3 37.4 ID CONC FEPT ABRCT ABRTT SEPT Animal identification Exposure concentration Body temperature CO before FEP recording Body temperature (C) before ABR click recording Body temperature (C) before ABR tone recording Body temperature (C) before SEP recording 03l05 ST* 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 2 of 3, Page: 169 Appendix G: Temperature Data (cont.) ID 90A3830 90A3831 90A3832 90A3833 90A3834 90A3837 90A3838 90A3839 90A3840 90A3841 90A3842 90A3843 90A3844 90A3845 90A3847 90A3848 90A3849 90A3850 90A3851 90A3852 90A3853 90A3855 90A3856 90A3857 90A3859 90A3860 90A3861 90A3862 90A3863 90A3864 90A3865 90A3867 90A3868 Six Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female GROUP CONC B 630 ppm B 630 ppm B 630 ppm B 630 ppm A 2000 ppm A 2000 ppm A 2000 ppm A 2000 ppm A 2000 ppm B 2000 ppm B 2000 ppm B 2000 ppm B 2000 ppm B 2000 ppm B 2000 ppm A 0 ppm A 0 ppm A 0 ppm A 0 ppm A 0 ppm A 0 ppm B 0 ppm B 0 ppm B 0 ppm B 0 ppm B 0 ppm B 0 ppm A 200 ppm A 200 ppm A 200 ppm A 200 ppm A 200 ppm A 200 ppm FEPT 38.1 38.4 37.4 38.0 37.5 38.1 38.2 36.9 38.4 38.4 38.0 37.5 37.8 37.6 37.5 37.6 38.4 37.8 38.6 37.4 37.8 37.5 38.6 37.2 38.2 37.6 37.7 37.7 38.0 38.2 38.1 37.5 38.1 ABRCT 38.1 38.7 37.7 37.8 37.7 38.7 37.9 37.7 39.0 38.6 38.4 37.9 38.2 38.5 37.6 37.7 38.0 38.5 39.0 37.7 37.7 37.2 39.0 37.9 38.5 37.3 38.4 38.0 37.9 38.0 38.3 37.6 38.2 ABRTT 38.1 38.7 37.7 37.8 37.7 38.7 37.9 37.6 38.9 38.5 38.5 37.9 38.3 38.4 37.6 37.7 38.0 38.4 39.0 37.7 37.7 37.1 39.0 38.0 38.5 37.2 38.4 38.0 37.9 38.0 38.3 37.4 38.2 SEPT 38.0 38.6 37.6 37.8 37.7 38.7 37.9 37.5 39.0 38.4 38.4 37.9 38.3 38.4 37.6 37.7 37.8 38.3 37.9 37.5 37.6 373 39.0 38.0 38.4 37.2 38.3 37.9 37.6 37.9 38.0 373 38.0 ID CONC FEFT ABRCT ABRTT SEPT Animal identification Exposure concentration Body temperature (0O before FEP recording Body temperature (C) before ABR click recording Body temperature (C) before ABR tone recording Body temperature (C) before SEP recording SL 031006 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 2 of 3, Page: 170 Appendix G, continued: Temperature Data ID 90A3869 90A3870 90A3872 90A3873 90A3874 90A3875 90A3876 90A3877 90A3878 90A3879 90A3880 90A3881 90A3883 90A3884 90A3885 90A3886 90A3887 90A3888 90A3890 90A3891 90A3892 90A3893 90A3895 90A3897 90A3898 90A3899 90A3900 90A3901 90A3902 90A3903 SEX Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female GROUP CONC B 200 ppm B 200 ppm B 200 ppm B 200 ppm B 200 ppm B 200 ppm A 630 ppm A 630 ppm A 630 ppm A 630 ppm A 630 ppm A 630 ppm B 630 ppm B 630 ppm B 630 ppm B 630 ppm B 630 ppm B 630 ppm A 2000 ppm A 2000 ppm A 2000 ppm A 2000 ppm A 2000 ppm B 2000 ppm B 2000 ppm B 2000 ppm B 2000 ppm B 2000 ppm B 2000 ppm B 2000 ppm FEPT 38.2 38.3 37.9 37.8 38.1 37.3 36.5 37.4 38.1 37.7 38.0 38.4 36.9 38.4 37.7 37.8 38.3 38.0 a 37.8 37.9 38.1 37.4 38.2 38.5 37.5 37.9 37.5 37.8 36.7 ABRCT 38.5 38.5 37.7 37.9 38.0 37.7 37.2 37.0 38.8 37.5 38.1 38.5 37.5 38.7 37.8 37.5 38.7 38.0 37.5 383 37.1 38.2 38.0 38.9 38.5 38.4 38.0 37.8 37.3 37.6 ABRTT 38.4 38.5 37.7 37.9 38.0 37.5 37.2 37.0 38.8 37.5 38.1 38.4 37.5 38.7 37.7 37.5 38.7 38.1 37.2 38.3 38.1 38.2 38.0 38.9 38.4 38.2 38.0 37.8 37.1 37.6 SEPT 38.4 38.7 37.8 37.9 38.0 37.7 37.1 37.0 38.9 37.5 38.2 38.4 37.7 38.7 37.3 37.3 38.6 38.2 37.4 383 38.0 38.1 37.9 39.0 38.1 383 37.9 37.7 37.1 37.6 a Temperature inadvertently not recorded ID CONC FEPT ABRCT ABRTT SEPT Animal identification Exposure concentration Body temperature (0O before FEP recording Body temperature (C) before ABR click recording Body temperature (C) before ABR tone recording Body temperature (C) before SEP recording SL 031007 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 2 of 3, Page: 171 Appendix G, continued: Temperature Data id 90A3792 90A3793 90A3795 90A3796 90A3797 90A3798 90A3799 90A3800 90A3802 90A3803 90A3804 90A3805 90A3806 90A3807 90A3808 90A3809 90A3810 90A3811 90A3813 90A3814 90A3815 90A3817 90A3818 90A3819 90A3820 90A3821 90A3822 90A3823 90A3824 90A3825 90A3828 90A3829 90A3830 90A3831 90A3832 SEX Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male GROUP A A A A A A B B B B B B A A A A A A B B B B B B A A A A A A B B B B B CONC 0 ppm 0 ppm 0 ppm 0 ppm 0 ppm 0 ppm 0 ppm 0 ppm 0 ppm 0 ppm 0 ppm 0 ppm 200 ppm 200 ppm 200 ppm 200 ppm 200 ppm 200 ppm 200 ppm 200 ppm 200 ppm 200 ppm 200 ppm 200 ppm 630 ppm 630 ppm 630 ppm 630 ppm 630 ppm 630 ppm 630 ppm 630 ppm 630 ppm 630 ppm 630 ppm ID CONC CNAPT CFRT Animal identification Exposure concentration Tail temperature (C) before CNAP recording Body temperature (C) before CFR recording CNAPT 25.2 25.0 25.4 25.2 24.8 24.7 24.9 25.0 24.8 25.0 25.0 25.1 25.1 25.1 25.1 25.0 24.9 25.8 25.7 24.9 25.8 25.0 24.4 24.8 25.3 26.4 25.1 25.1 24.8 25.2 25.3 25.1 25.1 25.0 25.0 CFRT 38.4 37.2 36.8 38.8 375 37.7 38.0 37.7 38.0 375 37.2 38.2 37.5 38.0 37.9 38.5 38.4 38.5 38.8 38.3 37.7 37.6 38.8 38.5 37.8 38.0 38.0 38.4 37.6 38.4 38.3 37.7 38.2 38.7 37.6 SL 031008 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 2 of 3, Page: 172 Appendix G, continued: Temperature Data id 90A3833 90A3834 90A3837 90A3838 90A3839 90A3840 90A3841 90A3842 90A3843 90A3844 90A3845 90A3847 90A3848 90A3849 90A3850 90A3851 90A3852 90A38S3 90A3855 90A3856 90A3857 90A3859 90A3860 90A3861 90A3862 90A3863 90A3864 90A3865 90A3867 90A3868 90A3869 90A3870 90A3872 90A3873 90A3874 SEX Male Male Male Male Male Male Male Male Male Male Male Male Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female GROUP B A A A A A A B B B B B A A A A A A B B B B B B A A A A A A B B B B B CONC 630 ppm 2000 ppm 2000 ppm 2000 ppm 2000 ppm 2000 ppm 2000 ppm 2000 ppm 2000 ppm 2000 ppm 2000 ppm 2000 ppm 0 ppm 0 ppm 0 ppm 0 ppm 0 ppm 0 ppm 0 ppm 0 ppm 0 ppm 0 ppm 0 ppm 0 ppm 200 ppm 200 ppm 200 ppm 200 ppm 200 ppm 200 ppm 200 ppm' 200 ppm 200 ppm 200 ppm 200 ppm ID CONC CNAPT CFRT Animal identification Exposure concentration Tail temperature (C) before CNAP recording Body temperature (C) before CFR recording CNAPT 24.8 2S.1 25.1 25.1 25.8 24.6 24.9 24.8 25.0 25.0 24.9 26.2 25.1 24.7 25.3 25.2 25.8 25.0 25.2 24.7 24.8 25.1 24.7 24.9 25.0 25.0 24.8 25.1 24.6 25.1 25.1 25.0 24.9 25.2 24.7 CFRT 37.8 373 38.7 37.8 37.9 38.9 38.6 38.4 37.8 38.2 37.4 37.7 37.7 38.0 38.5 38.9 373 37.8 373 39.1 377 38.4 37.4 38.4 37.6 38.2 37.1 383 373 383 38.4 38.6 37.7 38.0 38.0 031009 ST* 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 2 of 3, Page: 173 Appendix G, continued: Temperature Data ID 90A3875 90A3876 90A3877 90A3878 90A3879 90A3880 90A3881 90A3883 90A3884 90A3885 90A3886 90A3887 90A3888 90A3890 90A3891 90A3892 90A3893 90A3895 90A3897 90A3898 90A3899 90A3900 90A3901 90A3902 90A3903 SEX Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female GROUP CONC B 200 ppm A 630 ppm A 630 ppm A 630 ppm A 630 ppm A 630 ppm A 630 ppm B 630 ppm B 630 ppm B 630 ppm B 630 ppm B 630 ppm B 630 ppm A 2000 ppm A 2000 ppm A 2000 ppm A 2000 ppm A 2000 ppm B 2000 ppm B 2000 ppm B ' 2000 ppm B 2000 ppm B 2000 ppm B 2000 ppm B 2000 ppm ID CONC CNAPT CFRT Animal identification Exposure concentration Tail temperature (C> before CNAP recording Body temperature (C) before CFR recording CNAPT 24.7 25.0 25.1 25.0 24.8 25.2 25.1 24.8 25.1 24.5 25.0 25.3 24.7 25.0 24.7 25.0 24.8 24.8 24.8 24.8 25.0 25.0 25.1 24.5 25.0 CFRT 37.6 37.2 37.0 38.7 37.4 38.0 38.9 36.8 38.7 37.9 37.5 38.7 38.0 37.9 38.3 38.4 38.1 37.7 38.1 38.4 38.5 38.2 37.8 375 37.6 SL 031010 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 2 of 3, Page: 174 Appendix H: FEP-V Data ID 90A3792 90A3793 90A3795 90A3796 90A3797 90A3798 90A3799 90A3800 90A3802 90A3803 90A3804 90A3805 90A3806 90A3807 90A3808 90A3809 90A3810 90A3811 90A3813 90A3814 90A3815 90A3817 90A3818 90A3819 90A3820 90A3821 90A3822 90A3823 90A3824 90A3825 90A3828 90A3829 90A3830 90A3831 SEX Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male GROUP CONC A 0 ppm A 0 ppm A 0 ppm A 0 ppm A 0 ppm A 0 ppm B 0 ppm B 0 ppm B 0 ppm B 0 ppm B 0 ppm B 0 ppm A 200 ppm A 200 ppm A 200 ppm A 200 ppm A 200 ppm A 200 ppm B 200 ppm B 200 ppm B 200 ppm B 200 ppm B 200 ppm B 200 ppm A 630 ppm A 630 ppm A 630 ppm A 630 ppm A 630 ppm A 630 ppm B 630 ppm B 630 ppm B 630 ppm B 630 ppm FVSP 4.5 -1.8 4.5 -1.2 -0.6 0.3 1.5 2.7 -2.1 0.6 1.8 -2.4 3.3 0.3 -0.6 2.1 -1.5 -1.5 -14 -0.6 3.0 -3.0 -14 -3.3 14 -0.9 1.5 -0.6 -14 -0.3 -3.0 - -0.3 -1.2 -0.9 FVSC 0.604 0.638 0.919 0.877 0.692 0.602 0.690 0.748 0.374 0.930 0.645 0.614 0.799 0.798 0.627 0.714 0.649 0.804 0.387 0.441 0.613 0.359 0.359 0.803 0.724 0.842 0.830 0.876 0.445 0.730 0.578 0.635 0.658 0.642 FVSPw 19.70 24.77 14.44 15.92 28.33 17.06 18.41 1141 24.18 12.57 1104 24.60 16.35 19.83 17.83 26.58 13.64 3102 15.94 26.40 13.01 20.47 37.54 16.97 16.53 1183 10.31 2537 15.01 26.49 18.12 16.67 20.04 14.27 ID CONC FVSP FVSC FVSPw Animal identification Exposure concentration FEP-V 35-95.1 ms, latency difference from control template FEP-V 35-95.1 ms, optimized cross-correlation value (r) FEP-V 35-95.1 ms, power (RMS |iV) SL 0310H 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-00171W391 Volume 2 of 3, Page: 175 Appendix H, continued: FEP-V Data ID 90A3832 90A3833 90A3834 90A3837 90A3838 90A3839 90A3840 90A3841 90A3842 90A3843 90A3844 90A3845 90A3847 90A3848 90A3849 90A38S0 90A3851 90A3852 90A3853 90A3855 90A3856 90A3857 90A3859 90A3860 90A3861 90A3862 90A3863 90A3864 90A3865 90A3867 90A3868 90A3869 90A3870 90A3872 SEX Male Male Male Male Male Male Male Male Male Male Male Male Male Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female GROUP CONC B 630 ppm B 630 ppm A 2000 ppm A 2000 ppm A 2000 ppm A 2000 ppm A 2000 ppm B 2000 ppm B 2000 ppm B 2000 ppm B 2000 ppm B 2000 ppm B 2000 ppm A 0 ppm A 0 ppm A 0 ppm A 0 ppm A 0 ppm A 0 ppm B 0 ppm B 0 ppm B 0 ppm B 0 ppm B 0 ppm B 0 ppm A 200 ppm A 200 ppm A 200 ppm A 200 ppm A 200 ppm A 200 ppm B 200 ppm B 200 ppm B 200 ppm FVSP 0.6 -2.7 0.0 -3.6 -2.7 -14 -5.4 -2.7 3.3 4.2 -0.6 4.2 1.2 5.4 0.3 -1.2 -1.8 1.8 -0.9 4.2 -3.0 7.5 0.6 -2.4 1.8 -0.3 0.3 -0.6 0.6 -2.4 -2.4 ` -2.1 -0.6 1.2 FVSC 0.881 0.269 0.851 0.788 0.768 0.841 0.488 0.086 0.000 0.562 0.831 0.383 0.794 0.757 0.805 0.722 0.902 0.958 0.921 0.924 0.112 0.516 0.929 0.286 0.316 0.718 0.941 0.923 0.753 0.921 0.803 0.881 0.895 0.877 FVSPw 17.40 57.17 17.01 25.47 23.21 21.78 3331 55.08 20.98 17.02 19.14 10.13 11.48 23.48 50.84 14.69 19.08 19.10 15.64 16.13 40.92 22.70 9.28 37.94 651 28.14 11.14 19.15 8.11 15.96 39.86 18.47 17.27 38.91 ID CONC FVSP FVSC FVSPw Animal identification Exposure concentration FEP-V 35-95.1 ms, latencydifference from control template FEP-V 35-95.1 ms, optimized cross-correlation value (r) FEP-V 35*95.1 ms, power (RMS p.V) SL 031012 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 2 of 3, Page: 176 Appendix H, continued: FEF-V Data ID 90A3873 90A3874 90A3875 90A3876 90A3877 90A3878 90A3879 90A3880 90A3881 90A3883 90A3884 90A3885 90A3886 90A3887 90A3888 90A3890 90A3891 90A3892 90A3893 90A3895 90A3897 90A3898 90A3899 90A3900 90A3901 90A3902 90A3903 SEX Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female GROUP CONC 8 200 ppm B 200 ppm B 200 ppm A 630 ppm A 630 ppm A 630 ppm A 630 ppm A 630 ppm A 630 ppm B 630 ppm B 630 ppm B 630 ppm B 630 ppm B 630 ppm B 630 ppm A 2000 ppm A 2000 ppm A 2000 ppm A 2000 ppm A 2000 ppm B 2000 ppm B 2000 ppm B 2000 ppm B 2000 ppm B 2000 ppm B 2000 ppm B 2000 ppm FVSP -0.6 -3.3 -0.6 6.3 -0.9 -0.3 3.0 -2.1 -6.0 0.3 1.5 7.5b 6.0 0.0 2.7 0.3 0.9 -0.6 -0.9 -3.3 -4.8 -1.8 4.5 1.8 4.2 0.0 0.0 FVSC 0.895 0.581 0.655 0.735 0.903 0.634 0.735 0.959 0.512 0.668 0.498 0.305 0.679 0.593 0.824 0.645 0.648 0.660 0.850 0.791 0.845 0.708 0.741 0.869 0.579 0.613 0.927 b Outlier by F-Max test, see Table 38. ID CONC FVSP FVSC FVSPw Animal identification Exposure concentration FEP-V 35-95.1 ms, latency difference from control template FEP-V 35-95.1 ms, optimized cross-correlation value (r) FEP-V 35-95.1 ms, power (RMS |iV) FVSPw 33.54 17.08 24.14 10.88 19.39 19.72 16.40 9.66 24.96 11.77 21.00 16.58 19.26 13.05 9.37 33.99 22.49 14.13 1838 13.01 29.79 1033 28.88 18.74 24.33 11.82 10.11 0310X3 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 2 of 3, Page: 177 Appendix H, continued: FEP-V Data ID 90A3792 90A3793 90A3795 90A3796 90A3797 90A3798 90A3799 90A3800 90A3802 90A3803 90A3804 90A3805 90A3806 90A3807 90A3808 90A3809 90A3810 90A3811 90A3813 90A3814 90A3815 90A3817 90A3818 90A3819 90A3820 90A3821 90A3822 90A3823 90A3824 90A382S 90A3828 90A3829 SEX Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male GROUP CONC A 0 ppm A 0 ppm A 0 ppm A 0 ppm A 0 ppm A 0 ppm B 0 ppm B 0 ppm B 0 ppm B 0 ppm B 0 ppm B 0 ppm A 200 ppm A 200 ppm A 200 ppm A 200 ppm A 200 ppm A 200 ppm B 200 ppm B 200 ppm B 200 ppm B 200 ppm B 200 ppm B 200 ppm A 630 ppm A 630 ppm A 630 ppm A 630 ppm A 630 ppm A 630 ppm B 630 ppm B 630 ppm FVLP 16.5 -4.5 1.5 -3.0 3.0 -7.5 -19.5 3.0 -7.5 13.5 3.0 7.5 15.0 -18.0 0.0 -18.0 -9.0 -7.5 3.0 6.0 -6.0 1.5 -9.0 -1.5 -3.0 3.0 4.5 -10.5 -6.0 0.0 -3.0 3.0 FVLC 0.656b 0.989 0.988 0.966 0.992 0.976 0.604b 0.984 0.983 0.970 0.967 0.804 0.753 0.760 0.962 0.938 0.992 0.968 0.979 0.914 0.982 0.978 0.942 0.945 0.940 0.967 0.989 0.989 0.990 0.996 0.979 0.991 FVLPw 18.54 37.48 30.47 44.60 89.96 5Z79 25.56 41.78 67.33 21.60 29.69 62.93 37.05 27.36 79.18 28.19 39.97 39.46 44.61 48.41 3258 5950 79.62 33.76 31.99 38.18 18.69 63.09 40.77 42.68 4454 5859 N3AMP 29.1 74.1 54.9 105.8 166.0 105.9 53.7 78.7 135.3 14.7 46.0 126.8 34.1 515 162.4 51.7 94.3 79.9 90.4 75.5 79.5 118.0 137.8 65.9 65.5 86.6 42.0 122.7 80.9 85.6 77.6 118.6 b Outlier by F-Max test, see Table 38. ID CONC FVLP FVLC FVLPw N3AMP Animal identification Exposure concentration FEP-V 943-280.0 ms, latency difference from control template FEP-V 943-280.0 ms, optimized cross-correlation value (r) FEP-V 943-280.0 ms, power (RMS uV) N3 to baseline peak amplitude SL 031014 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 2 of 3, Page: 17S Appendix H, continued: FEP-V Data ID 90A3830 90A3831 90A3832 90A3833 90A3834 90A3837 90A3838 90A3839 90A3840 90A3841 90A3842 90A3843 90A3844 90A3845 90A3847 90A3848 90A3849 90A3850 90A3851 90A3852 90A3853 90A3855 90A3856 90A3857 90A38S9 90A3860 90A3861 90A3862 90A3863 90A3864 90A3865 SEX Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female GROUP CONC B 630 ppm B 630 ppm B 630 ppm B 630 ppm A 2000 ppm A 2000 ppm A 2000 ppm A 2000 ppm A 2000 ppm B 2000 ppm B 2000 ppm B 2000 ppm B 2000 ppm B 2000 ppm B 2000 ppm A 0 ppm A 0 ppm A 0 ppm A 0 ppm A 0_ppm A 0 ppm B 0 ppm B 0 ppm B 0 ppm B 0 ppm B 0 ppm B 0 ppm A 200 ppm A 200 ppm A 200 ppm A 200 ppm FVLP -3.0 -9.0 -1.5 15.0 6.0 3.0 -6.0 -7.5 10.5 6.0 -3.0 1.5 -21.0 16.5 7.5 3.0 6.0 6.0 -9.0 0.0 -13.5 1.5 1.5 -6.0 -4.5 0.0 4.5 -19.5 -13.5 -12.0 -22.5 FVL 0.973 0.976 0.957 0.871 0.979 0.964 0.968 0.860 0.921 0.936 0.978 0.949 0.957 0.948 0.898 0.976 0.912 0.994 0.960 0.984 0.972 0.975 0.924 0.000b 0.982 0.952 0.982 0.431b 0.945 0.959 0.858 FVLPw 66.77 105.97 31.42 74.54 38.28 68.73 78.43 14.41 51.67 110.95 74.62 24.09 28.81 32.11 38.66 47.23 27.66 38.40 23.81 37.12 29.39 30.24 64.47 19.95 37.66 68.79 60.71 19.52 23.67 49.21 16.81 N3AMP 137.4 211.0 47.8 104.6 71.6 122.5 178.9 22.0 46.9 146.7 139.4 47.9 61.9 51.4 56.4 82.8 53.8 20.3 9.0 66.7 71.9 51.1 126.2 0.0 58.8 78.9 106.2 36.1 45.3 89.2 33.1 b Outlier by F-Max test, see Table 38. ID CONC FVLP FVLC FVLPw N3AMP Animal identification Exposure concentration FEP-V 943-280.0 ms, latency difference from control template FEP-V 94.5-280.0 ms, optimized cross-correlation value (r) FEP-V 943-280.0 ms, power (RMS pV) N3 to baseline peak amplitude 031015 Si; 1,1,t-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 2 of 3, Page: 179 Appendix H, continued: FEP-V Data ID 90A3867 90A3868 90A3869 90A3870 90A3872 90A3873 90A3874 90A3875 90A3876 90A3877 9QA3S7S 90A3879 90A3880 90A3881 90A3883 90A3884 90A3885 90A3886 90A3887 90A3888 90A3890 90A3891 90A3892 90A3893 90A3895 90A3897 90A3898 90A3899 90A3900 90A3901 90A3902 90A3903 SEX Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female GROUP CONC A 200 ppm A 200 ppm B 200 ppm B 200 ppm B 200 ppm B 200 ppm B 200 ppm B 200 ppm A 630 ppm A 630 ppm A 630 ppm A 630 ppm A 630 ppm A 630 ppm B 630 ppm B 630 ppm B 630 ppm B 630 ppm B 630 ppm B 630 ppm A 2000 ppm A 2000 ppm A 2000 ppm A 2000 ppm A 2000 ppm B 2000 ppm B 2000 ppm B 2000 ppm B 2000 ppm B 2000 ppm B 2000 ppm B 2000 ppm FVLP -15.0 -24.0 -1.5 -16.5 -6.0 -16.5 -3.0 -7.5 18.0 -12.0 0.0 -19.5 -19.5 -4.5 -3.0 -18.0 13.5 4.5 0.0 4.5 -18.0 -18.0 -6.0 -6.0 -7.5 -16.5 -4.5 12.0 6.0 -13.5 -9.0 -12.0 FVLC 0.972 0.364b 0.932 0.883 0.727 0.925 0.961 0.971 0.936 0.950 0.810 0.695 0.935 0.942 0.987 0.912 0.797 0.907 0.988 0.982 0.874 0.894 0.743 0.972 0.929 0.971 0.987 0.922 0.980 Q.366b 0.989 0.924 FVLPw 5176 29.79 22.95 27.24 38.96 51.10 26.27 55.78 13.71 31.21 19.57 24.34 32.14 46.13 44.51 65.80 24.69 40.43 57.94 34.72 52.82 56.15 29.49 57.07 2855 84.66 47.68 20.92 36.60 18.07 37.59 19.00 N3AMP 134.9 61.1 19.9 41.0 61.8 84.1 23.6 122.8 22.2 65.8 27.7 219 65.4 68.7 77.3 141.4 40.8 58.8 104.6 70.1 113.2 125.2 50.6 84.3 514 158.3 78.6 118 50.4 20.6 75.4 28.1 b Outlier by F-Max test, see Table 38. ID CONC FVLP FVL.C FVLPw N3AMP Animal identification Exposure concentration FEP-V 94.5-280.0 ms, latency difference from control template FEP-V 94.5-280.0 ms, optimized cross-correlation value (r) FEP-V 945-280.0 ms, power (RMS pV) N3 to baseline peak amplitude SL 031016 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 2 of 3, Page: 180 Appendix I: SEP-C Data ID 90A3792 90A3793 90A3795 90A3796 90A3797 90A3798 90A3799 90A3800 90A3802 90A3803 90A3804 90A3805 90A3806 90A3807 90A3808 90A3809 90A3810 90A3811 90A3813 90A3814 90A3815 90A3817 90A3818 90A3819 90A3820 90A3821 90A3822 90A3823 90A3824 90A3825 90A3828 90A3829 90A3830 90A3831 SEX Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male GROUP CONC A 0 ppm A 0 ppm A 0 ppm A 0 ppm A 0 ppm A 0 ppm B 0 ppm B 0 ppm B 0 ppm B 0 ppm B 0 ppm B 0 ppm A 200 ppm A 200 ppm A 200 ppm A 200 ppm A 200 ppm A 200 ppm B 200 ppm B 200 ppm B 200 ppm B 200 ppm B 200 ppm B 200 ppm A 630 ppm A 630 ppm A 630 ppm A 630 ppm A 630 ppm A 630 ppm B 630 ppm B 630 ppm B 630 ppm B 630 ppm SEPCSP -0.28 -0.21 0.70 -0.35 0.28 -0.14 0.56 0.42 -0.49 -0.28 0.00 -0.35 0.42 -0.28 -0.56 -0.63 -0.28 0.14 -0.21 -0.28 -0.56 -0.56 -0.42 0.21 0.70 0.07 0.28 -0.42 -0.07 0.07 -0.28 0.21 -0.35 -0.14 SEPCSC 0.956 0.895 0.897 0.857 0.808 0.920 0.807 0.961 0.903 0.928 0.830 0.855 0.922 0.955 0.781 0.813 0.935 0.902 0.972 0.844 0.890 0.883 0.927 0.856 0.778 0.848 0.827 0.856 0.886 0.927 0.901 0.890 0.824 0.845 SEPCSPw 8.71 5.36 4.30 12.02 7.54 7.65 9.11 6.98 5.29 6.84 5.59 8.38 4.35 6.35 9.21 10.16 8.89 14.07 8.76 8.71 626 739 13.22 2.87 7.44 7.16 6.08 627 9.19 735 10.88 3.49 4.81 8.11 ID CONC SEPCSP SEPCSC SEPCSPw Animal identification Exposure concentration SEP-C 4.0*18.0 ms, latency difference from control template SEP-C 4.0-18.0 ms, optimized cross-correlation value (r) SEP-C 4.0-18.0 ms, power (RMS jiV) 031017 5L 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 2 of 3, Page: 181 Appendix I, continued: SEP-C Data ID 90A3832 90A3833 90A3834 90A3837 90A3838 90A3839 90A3840 90A3841 90A3842 90A3843 90A3844 90A3845 90A3847 90A3848 90A3849 90A3850 90A3851 90A3852 90A3853 90A3855 90A3856 90A3857 90A3859 90A3860 90A3861 90A3862 90A3863 90A3864 90A3865 90A3867 90A3868 90A3869 90A3870 90A3872 SEX Male Male Male Male Male Male Male Male Male Male Male Male Male Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female GROUP CONC B 630 ppm B 630 ppm A 2000 ppm A 2000 ppm A 2000 ppm A 2000 ppm A 2000 ppm B 2000 ppm B 2000 ppm B 2000 ppm B 2000 ppm B 2000 ppm B 2000 ppm A 0 ppm A 0 ppm A 0 ppm A 0 ppm A 0 ppm A 0 ppm B 0 ppm B 0 ppm B 0 ppm B 0 ppm B 0 ppm B 0 ppm A 200 ppm A 200 ppm A 200 ppm A 200 ppm A 200 ppm A 200 ppm B 200 ppm B 200 ppm B 200 ppm SEPCSP 0.00 0.70 0.00 0.70 0.21 0.21 -0.63 0.42 0.35 -0.14 0.07 -0.42 -0.21 0.42 0.21 035 -0.21 0.00 0.63 -0.35 -0.21 -0.07 0.35 0.21 -0.14 0.14 0.28 -0.07 0.00 0.70 0.63 0.14 0.14 -0.14 SEPCSC 0.892 0.686 0.925 0.804 0.960 0.843 0.596 0.859 0.917 0.730 0.944 0.887 0.829 0.723 0.704 0.878 0.885 0.331 0.756 0.765 0.704 0.917 0.649 0.359 0.864 0.739 0.689 0.825 0.865 0.841 0.742 0.868 0.879 0.798 SEPCSPw 7.34 4.17 4.74 8.70 7.03 6.65 4.85 8.16 16.80 8.14 11.97 12.31 6.74 6.15 8.40 4.73 9.26 4.00 4.61 4.82 8.45 8.57 6.57 6.17 8.56 11.04 179 7.96 6.38 4.66 10.86 7.01 6.72 6.56 ID CONC SEPCSP SEPCSC SEPCSPw Animal identification Exposure concentration SEP-C 4.0-18.0 ms, latency difference from control template SEP-C 4.0-18.0 ms, optimized cross-correlation value (r) SEP-C 4.0-18.0 ms, power (RMS pV> SL 031018 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 2 of 3, Page: 182 Appendix I, continued: SEP-C Data IQ 90A3873 90A3874 90A3875 90A3876 90A3877 90A3878 90A3879 90A3880 90A3881 90A3883 90A3884 90A3885 90A3886 90A3887 90A3888 90A3890 90A3891 90A3892 90A3893 90A3895 90A3897 90A3898 90A3899 90A3900 90A3901 90A3902 90A3903 SEX Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female GROUP CONC B 200 ppm B 200 ppm B 200 ppm A 630 ppm A 630 ppm A 630 ppm A 630 ppm A 630 ppm A 630 ppm B 630 ppm B 630 ppm B 630 ppm B 630 ppm B 630 ppm B 630 ppm A 2000 ppm A 2000 ppm A 2000 ppm A 2000 ppm A 2000 ppm B 2000 ppm B 2000 ppm B 2000 ppm B 2000 ppm B 2000 ppm B 2000 ppm B 2000 ppm SEPCSP 0.28 0.21 0.28 0.49 0.35 -0.28 0.00 0.28 0.77 0.00 0.35 0.14 0.28 0.35 0.14 0.00 035 0.42 0.28 0.28 -0.14 0.49 0.00 0.35 -0.07 0.28 0.14 SEPCSC 0.763 0.756 0.683 0.838 0.528 0.866 0.644 0.872 0.925 0.745 0.806 0.738 0.691 0.805 0.743 0.407 0.927 0.790 0.818 0.912 0.949 0.509 0.847 0.846 0.880 0.883 0.676 SEPCSPw 11.66 4.40 9.92 4.36 1.92 9.27 7.79 7.23 6.71 6.84 5.08 5.00 3.44 9.18 9.39 7.63 12.09 737 7.26 7.39 13.95 7.86 8.33 6.92 434 535 935 ID CONC SEPCSP SEPCSC SEPCSPw Animal identification Exposure concentration SEP-C 4.0-18.0 ms, latency difference from control template SEP-C 4.0-18.0 ms, optimized cross-correlation value (r) SEP-C 4.0-18.0 ms, power (RMS p,V) SL 031019 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 2 of 3, Page: 183 Appendix I, continued: SEF-C Data ID 90A3792 90A3793 90A3795 90A3796 90A3797 90A3798 90A3799 90A3800 90A3802 90A3803 90A3804 90A3805 90A3806 90A3807 90A3808 90A3809 90A3810 90A3811 90A3813 90A3814 90A3815 90A3817 90A3818 90A3819 90A3820 90A3821 90A3822 90A3823 90A3824 90A3825 90A3828 90A3829 90A3830 90A3831 SEX Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male GROUP CONC A 0 ppm A 0 ppm A 0 ppm A 0 ppm A 0 ppm A 0 ppm B 0 ppm B 0 ppm B 0 ppm B 0 ppm B 0 ppm B 0 ppm A 200 ppm A 200 ppm A 200 ppm A 200 ppm A 200 ppm A 200 ppm B 200 ppm B 200 ppm B 200 ppm B 200 ppm B 200 ppm B 200 ppm A 630 ppm A 630 ppm A 630 ppm A 630 ppm A 630 ppm A 630 ppm B 630 ppm B 630 ppm B 630 ppm B 630 ppm SEPCLP 0.0 0.4 -1.2 -1.2 1.2 -2.0 1.6 1.6 -2.4 -0.4 0.4 0.8 1.2 -1.2 2.4 1.2 -1.2 0.0 0.0 -1.2 0.8 -0.8 -1.6 -0.8 0.4 2.4 -0.4 -1.6 -0.8 2.0 2.8 - -2.0 -0.4 1.2 SEPCLC 0.958 0.982 0.981 0.994 0.959 0.982 0.979 0.980 0.995 0.993 0.990 0.973 0.976 0.969 0.982 0.954 0.973 0.983 0.978 0.980 0.942 0.955 0.994 0.985 0.996 0.910 0.988 0.956 0.980 0.987 0.981 0.972 0.944 0.982 SEPCLPw 23.91 28.16 21.79 33.92 22.33 34.46 2750 25.92 26.20 24.73 30.63 35.67 22.97 31.71 35.29 28.29 34.63 26.02 35.00 31.61 23.70 29.62 37.44 26.11 27.96 29.67 34.10 28.06 33.85 41.34 33.22 19.64 28.13 31.22 ID CONC SEPCLP SEPCLC SEPCLPw Animal identification Exposure concentration SEP-C 18.0-70.0 ms, latency difference from control template SEP-C 18.0-70.0 ms, optimized cross-correlation value (r) SEP-C 18.0-70.0 ms, power (RMS pV) SL 031020 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 2 of 3, Page: 184 Appendix I, continued: SEP-C Data ID 90A3832 90A3833 90A3834 90A3837 90A3838 90A3839 90A3840 90A3841 90A3842 90A3843 90A3844 90A3845 90A3847 90A3848 90A3849 90A3850 90A3851 90A3852 90A3853 90A3855 90A3856 90A3857 90A3859 90A3860 90A3861 90A3862 90A3863 90A3864 90A3865 90A3867 90A3868 90A3869 90A3870 90A3872 SEX Male Male Male Male Male Male Male Male Male Male Male Male Male Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female GROUP CONC 5 630 ppm 8 630 ppm A 2000 ppm A 2000 ppm A 2000 ppm A 2000 ppm A 2000 ppm B 2000 ppm B 2000 ppm B 2000 ppm B 2000 ppm B 2000 ppm B 2000 ppm A 0 ppm A 0 ppm A 0 ppm A 0 ppm A 0 ppm A 0 ppm B 0 ppm B 0 ppm B 0 ppm B 0 ppm B 0 ppm B 0 ppm A 200 ppm A 200 ppm A 200 ppm A 200 ppm A 200 ppm A 200 ppm B 200 ppm B 200 ppm B 200 ppm SEPCLP -0.4 1.2 0.8 2.0 0.0 0.4 -0.8 1.2 1.6 0.4 0.4 0.8 0.0 0.8 0.8 -0.4 -1.6 0.4 0.8 1.2 -1.2 -0.4 -2.8 0.4 0.4 0.4 -0.4 -2.4 -1.6 0.0 0.0 * 0.0 -0.8 -0.4 SEPCLC 0.992 0.983 0.946 0.987 0.984 0.987 0.934 0.987 0.976 0.986 0.985 0.989 0.896 0.979 0.882 0.989 0.963 0.976 0.952 0.885 0.955 0.993 0.912 0.970 0.978 0.947 0.974 0.849 0.983 0.994 0.939 0.941 0.959 0.981 SEPCLPw 34.23 33.70 26.45 3034 33.19 27.09 4239 29.83 39.05 30.65 30.61 3033 15.72 28.00 25.58 5036 36.45 25.81 2231 25.19 4433 30.63 39.78 3138 3838 54.60 24.93 32.68 46.41 31.72 37.45 27.45 3238 34.18 ID CONC SEPCLP SEPCLC SEPCLPw Animal identification Exposure concentration SEP-C 18.0-70.0 ms, latency difference from control template SEP-C 18.0-70.0 ms, optimized cross-correlation value (r) SEP-C 18.0-70.0 ms, power (RMS |iV) Si 03102l 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 2 of 3, Page: 185 Appendix I, continued: SEP-C Data ID 90A3873 90A3874 90A3875 90A3876 90A3877 90A3878 90A3879 90A3880 90A3881 90A3883 90A3884 90A3885 90A3886 90A3887 90A3888 90A3890 90A3891 90A3892 90A3893 90A3895 90A3897 90A3898 90A3899 90A3900 90A3901 90A3902 90A3903 SEX Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female GROUP CONC B 200 ppm B 200 ppm B 200 ppm A 630 ppm A 630 ppm A 630 ppm A 630 ppm A 630 ppm A 630 ppm B 630 ppm B 630 ppm B 630 ppm B 630 ppm B 630 ppm B 630 ppm A 2000 ppm A 2000 ppm A 2000 ppm A 2000 ppm A 2000 ppm B 2000 ppm B 2000 ppm B 2000 ppm B 2000 ppm B 2000 ppm B 2000 ppm B 2000 ppm SEPCLP 0.0 -0.8 2.8 -0.4 -0.8 1.2 0.4 -1.6 -0.8 1.2 0.4 -1.6 0.0 -0.4 0.0 0.4 1.6 0.4 0.8 -1.6 -0.8 2.0 0.4 -0.4 -2.0 -0.4 0.8 SEPCLC 0.964 0.984 0.976 0.961 0.978 0.990 0.977 0.937 0.969 0.992 0.991 0.902 0.971 0.965 0.991 0.966 0.955 0.987 0.993 0.967 0.969 0.917 0.981 0.901 0.948 0.979 0.977 SEPCLPw 40.78 37.24 33.43 25.56 32.70 48.42 3151 29.18 37.11 36.91 32.64 27.41 22.07 33.10 61.19 31.23 36.00 42.75 45.42 3851 42.19 5Z22 37.94 2457 26.51 25.85 49.72 ID CONC SEPCLP SEPCLC SEPCLPw Animal identification Exposure concentration SEP-C 18.0-70.0 ms, latency difference from control template SEP-C 18.0-70.0 ms, optimized cross-correlation value (r) SEP-C 18.0-70.0 ms, power (RMS pV) s*- 031022 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 2 of 3, Page: 186 Appendix J: SEP-S Data ID 90A3792 90A3793 90A3795 90A3796 90A3797 90A3798 90A3799 90A3800 90A3802 90A3803 90A3804 90A3805 90A3806 90A3807 90A3808 90A3809 90A3810 90A3811 90A3813 90A3814 90A3815 90A3817 90A3818 90A3819 90A3820 90A3821 90A3822 90A3823 90A3824 90A3825 90A3828 90A3829 90A3830 90A3831 SEX Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male GROUP CONC A 0 ppm A 0 ppm A 0 ppm A 0 ppm A 0 ppm A 0 ppm B 0 ppm B 0 ppm B 0 ppm B 0 ppm B 0 ppm B 0 ppm A 200 ppm A 200 ppm A 200 ppm A 200 ppm A 200 ppm A 200 ppm B 200 ppm B 200 ppm B 200 ppm B 200 ppm B 200 ppm B 200 ppm A 630 ppm A 630 ppm A 630 ppm A 630 ppm A 630 ppm A 630 ppm B 630 ppm B 630 ppm B 630 ppm B 630 ppm SEPSSP -0.28 -0.14 1.47 0.07 0.35 0.70 0.91 0.84 -0.77 -0.49 -0.56 -0.56 1.61 0.70 -0.42 -0.77 -0.35 0.42 -1.40 -1.75 0.00 -1.26 -0.35 -1.75 0.56 0.77 0.77 0.21 0.56 -0.91 -0.70 - 0.00 0.42 0.56 SEPSSC 0.856 0.958 0.863 0.964 0.978 0.968 0.000 0.975 0.925 0.980 0.438 0.891 0.000 0.960 0.907 0.943 0.974 0.988 0.152 0.000 0.968 0.350 0.982 0.000 0.978 0.928 0.978 0.976 0.906 0.000 0.317 0.232 0.799 0.955 SEPSSPw 4.91 9.15 7.30 11.27 11.93 7.12 4.61 9.00 4.75 9.40 3.33 4.08 3.13 6.28 6.02 6.14 8.31 13.16 3.96 9.61 5.86 3.19 13.05 6.25 6.46 8.71 7.16 14.27 8.40 6.21 3.61 1.65 5.44 11.27 ID CONC SEPSSP SEPSSC SEPSSPw Animal identification Exposure concentration SEP-S 4.0-16.5 ms, latency difference from control template SEP-S 4.0-163 ms, optimized cross-correlation value (r) SEP-S 4.0-16.5 ms, power (RMS pV) SL 031023 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 2 of 3, Page: 187 Appendix J, continued: SEP-S Data ID 90A3832 90A3833 90A3834 90A3837 90A3838 90A3839 90A3840 90A3841 90A3842 90A3843 90A3844 90A3845 90A3847 90A3848 90A3849 90A3850 90A3851 90A3852 90A3853 90A3855 90A3856 90A3857 90A3859 90A3860 90A3861 90A3862 90A3863 90A3864 90A3865 90A3867 90A3868 90A3869 90A3870 90A3872 SEX Male Male Male Male Male Male Male Male Male Male Male Male Male Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female GROUP CONC B 630 ppm B 630 ppm A 2000 ppm A 2000 ppm A 2000 ppm A 2000 ppm A 2000 ppm B 2000 ppm B 2000 ppm B 2000 ppm B 2000 ppm B 2000 ppm B 2000 ppm A 0 ppm A 0 ppm A 0 ppm A 0 ppm A 0 ppm A 0 ppm B 0 ppm B O'ppm B 0 ppm B 0 ppm B 0 ppm B 0 ppm A 200 ppm A 200 ppm A 200 ppm A 200 ppm A 200 ppm A 200 ppm B 200 ppm B 200 ppm B 200 ppm SEPSSP -0.07 1.19 0.00 -180 -0.28 0.63 -1.82 -0.77 -0.84 0.14 0.28 -0.42 0.21 0.63 0.84 0.00 -0.14 -0.28 0.00 0.00 -1.19 -0.42 0.21 0.00 -0.07 0.00 0.07 0.35 0.00 0.00 0.49 -0.42 0.00 -0.14 SEPSSC 0.979 0.000 0.885 0.587 0.971 0.967 0.000 0.000 0.941 0.528 0.801 0.964 0.990 0.808 0.755 0.528 0.896 0.442 0.668 0.527 0.000 0.777 0.955 0.000 0.892 0.860 0.945 0.784 0.000 0.779 0.928 0.816 0.766 0.861 SEPSSPw 9.43 5.77 3.59 5.87 9.62 9.16 9.92 9.42 8.15 2.06 3.62 8.25 6.91 7.27 9.72 4.84 7.45 3.92 4.71 7.42 12.48 13.78 6.57 9.87 9.41 8.95 3.11 8.94 11.71 8.97 16.45 5.96 8.10 11.92 ID CONC SEPSSP SEPSSC SEPSSPw Animal identification Exposure concentration SEP-S 4.0-16.5 ms, latency difference from control template SEP-S 4.0-163 ms, optimized cross-correlation value (r) SEP-S 4.0-16.5 ms, power (RMS uV) SL 3l024 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 2 of 3, Page: 188 Appendix J, continued: SEP-S Data ID 90A3873 90A3874 90A3875 90A3876 90A3877 90A3878 90A3879 90A3880 90A3881 90A3883 90A3884 90A3885 90A3886 90A3887 90A3888 90A3890 90A3891 90A3892 90A3893 90A3895 90A3897 90A3898 90A3899 90A3900 90A3901 90A3902 90A3903 SEX Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female GROUP CONC B 200 ppm B 200 ppm B 200 ppm A 630 ppm A 630 ppm A 630 ppm A 630 ppm A 630 ppm A 630 ppm B 630 ppm B 630 ppm B 630 ppm B 630 ppm B 630 ppm B 630 ppm A 2000 ppm A 2000 ppm A 2000 ppm A 2000 ppm A 2000 ppm B 2000 ppm B 2000 ppm B 2000 ppm B 2000 ppm B 2000 ppm B 2000 ppm B 2000 ppm SEPSSP -0.21 0.00 0.21 1.40 0.21 -0.42 0.35 0.21 -1.12 -0.70 -0.35 0.00 0.00 -0.21 -0.21 0.21 0.14 0.49 -0.56 -2.80b -0.14 0.70 -0.35 0.00 0.00 0.56 0.77 SEPSSC 0.851 0.000 0.874 0.891 0.723 0.807 0.772 0.846 0.000 0.872 0.701 0.872 0.000 0.800 0.924 0.739 0.834 0.891 0.908 0.273 0.859 0.861 0.633 0.256 0.777 0.000 0.937 SEPSSPw 6.75 8.77 4.41 7.33 3.02 20.03 11.16 9.85 8.26 11.64 1187 8.52 11.57 19.08 7.74 8.90 10.19 636 837 439 15.47 1032 8.21 6.41 190 739 10.10 b Outlier by F-Max test, see Table 38. ID CONC SEPSSP SEPSSC SEPSSPw Animal identification Exposure concentration SEP-S 4.0-16.5 ms, latency difference from control template SEP-S 4.0-165 ms, optimized cross-correlation value (r) SEP-S 4.0-16.5 ms, power (RMS jiV) SL 031025 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 2 of 3, Page: 189 Appendix }, continued: SEP-S Data IB 90A3792 90A3793 90A3795 90A3796 90A3797 90A3798 90A3799 90A3800 90A3802 90A3803 90A3804 90A3805 90A3806 90A3807 90A3808 90A3809 90A3810 90A3811 90A3813 90A3814 90A3815 90A3817 90A3818 90A3819 90A3820 90A3821 90A3822 90A3823 90A3824 90A3825 90A3828 90A3829 90A3830 90A3831 Six Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male GROUP CONC A 0 ppm A 0 ppm A 0 ppm A 0 ppm A 0 ppm A 0 ppm B 0 ppm B 0 ppm B 0 ppm B 0 ppm B 0 ppm B 0 ppm A 200 ppm A 200 ppm A 200 ppm A 200 ppm A 200 ppm A 200 ppm B 200 ppm B 200 ppm B 200 ppm B 200 ppm B 200 ppm B 200 ppm A 630 ppm A 630 ppm A 630 ppm A 630 ppm A 630 ppm A 630 ppm B 630 ppm B 630 ppm B 630 ppm B 630 ppm SEPSLP -0.8 -2.4 1.2 -0.4 -0.4 2.0 0.4 6.4 -1.2 0.0 -2.0 -1.6 0.0 -0.4 -2.0 -1.2 -0.8 0.4 -1.2 -7.2 -0.8 -2.8 -1.2 -4.0 3.2 0.8 0.0 -0.4 0.0 -4.8 -1.6 - -0.4 2.4 0.8 SEPSLC 0.988 0.965 0.972 0.994 0.969 0.983 0.943 0.962 0.982 0.980 0.909 0.990 0.948 0.927 0.972 0.991 0.960 0.946 0.980 0.942 0.964 0.964 0.972 0.938 0.909 0.966 0.975 0.982 0.967 0.965 0.974 0.964 0.953 0.985 SEPSLPw 64.52 76.79 51.03 75.29 96.59 63.81 58.78 57.51 40.48 65.69 44.29 56.04 32.83 41.86 71.98 48.79 53.84 60.22 46.97 65.18 50.67 53.59 57.54 45.29 5231 75.01 55.42 76.77 76.18 64.65 4631 52.93 67.00 62.12 ID CONC SEPSLP SEPSLC SEPSLPw Animal identification Exposure concentration SEP-S 16-5-90.0 ms, latency difference from control template SEP-S 16.5-90.0 ms, optimized cross-correlation value (r) SEP-S 16.5-90.0 ms, power (RMS jiV) SL 031026 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 2 of 3, Page: 190 Appendix J, continued: SEP-S Data ID 90A3832 90A3833 90A3834 90A3837 90A3838 90A3839 90A3840 90A3841 90A3842 90A3843 90A3844 90A3845 90A3847 90A3848 90A3849 90A3850 90A38S1 90A3852 90A3853 90A3855 90A3856 90A3857 90A38S9 90A3860 90A386I 90A3862 90A3863 90A3864 90A3865 90A3867 90A3868 90A3869 90A3870 90A3872 SEX Male Male Male Male Male Male Male Male Male Male Male Male Male Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female GROUP CONC B 630 ppm B 630 ppm A 2000 ppm A 2000 ppm A 2000 ppm A 2000 ppm A 2000 ppm B 2000 ppm B 2000 ppm B 2000 ppm B 2000 ppm B 2000 ppm B 2000 ppm A 0 ppm A 0 ppm A 0 ppm A 0 ppm A 0 ppm A 0 ppm B 0 ppm B 0 ppm B Oppm B 0 ppm B 0 ppm B 0 ppm A 200 ppm A 200 ppm A 200 ppm A 200 ppm A 200 ppm A 200 ppm B 200 ppm B 200 ppm B 200 ppm SEPSLP -0.4 -5.2 -1.2 -3.2 -0.4 0.8 -8.0 -6.4 -1.2 0.0 -0.4 -0.4 0.8 5.2 2.0 -1.6 4.4 -0.8 0.8 0.0 -2.4 1.6 -2.0 -6.8 0.4 4.4 -0.8 -0.4 -4.4 -1.2 0.8 -0.8 2.4 0.8 SEPSLC 0.985 0.853 0.963 0.971 0.990 0.967 0.933 0.890 0.896 0.979 0.970 0.981 0.984 0.942 0.881 0.987 0.979 0.975 0.978 0.989 0.807 0.986 0.981 0.967 0.989 0.968 0.975 0.973 0.866 0.967 0.985 0.975 0.943 0.976 SEPSLPw 50.51 44.35 4738 92.52 55.61 49.39 75.04 78.57 5636 55.07 52.43 68.51 55.64 5739 91.15 6232 6535 5434 56.07 63.47 91.14 8634 59.40 86.90 59.72 63.18 44.64 85.91 70.11 82.84 81.73 71.08 46.67 72.48 ID CONC SEPSLP SEPSLC SEPSLPw Animal identification Exposure concentration SEP-S 16.5-90.0 ms, latency difference from control template SEP-S 163-90.0 ms, optimized cross-correlation value (r) SEP-S 163-90.0 ms, power(RMS pV) SL 031027 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 2 of 3, Page: 191 Appendix J, continued: SEP-S Data ID 90A3873 90A3874 90A3875 90A3876 90A3877 90A3878 90A3879 90A3880 90A3881 90A3883 90A3884 90A3885 90A3886 90A3887 90A3888 90A3890 90A3891 90A3892 90A3893 90A3895 90A3897 90A3898 90A3899 90A3900 90A3901 90A3902 90A3903 SEX Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female GROUP CONC B 200 ppm B 200 ppm B 200 ppm A 630 ppm A 630 ppm A 630 ppm A 630 ppm A 630 ppm A 630 ppm B 630 ppm B 630 ppm B 630 ppm B 630 ppm B 630 ppm B 630 ppm A 2000 ppm A 2000 ppm A 2000 ppm A 2000 ppm A 2000 ppm B 2000 ppm B 2000 ppm B 2000 ppm B 2000 ppm B 2000 ppm B 2000 ppm B 2000 ppm SEPSLP 0.8 -8.0 1.2 2.8 -1.6 0.0 4.8 -0.8 -6.8 -1.6 -0.8 0.8 -3.6 1.2 -2.4 2.8 2.8 0.4 -0.4 -4.4 -0.8 2.0 0.8 -2.0 0.8 -1.6 -0.4 SEPSLC 0.966 0.933 0.932 0.984 0.984 0.958 0.931 0.953 0.932 0.944 0.960 0.952 0.980 0.972 0.975 0.982 0.960 0.961 0.987 0.950 0.985 0.968 0.992 0.993 0.901 0.993 0.963 SEPSLPw 53.21 74.78 67.20 55.62 66.75 102.01 61.45 52.25 92.00 54.11 81.13 34.95 67.39 90.77 46.49 88.20 75.84 68.46 83.36 64.76 90.66 66.58 61.73 97.86 32.09 65.91 55.77 ID CONC SEPSLP SEPSLC SEPSLPw Animal identification Exposure concentration SEP-S 16.5-90.0 ms, latency difference from control template SEP-S 16.5-90.0 ms, optimized cross-correlation value (r) SEP-S 16-5-90.0 ms, power (RMS |iV) SL 031028 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 2 of 3, Page: 192 Appendix K: ABR dick Data ID 90A3792 90A3793 90A3795 90A3796 90A3797 90A3798 90A3799 90A3800 90A3802 90A3803 90A3804 90A3805 90A3806 90A3807 90A3808 90A3809 90A3810 90A3811 90A3813 90A3814 90A3815 90A3817 90A3818 90A3819 90A3820 90A3821 90A3822 90A3823 90A3824 90A3825 90A3828 90A3829 90A3830 90A3831 SEX Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male GROUP CONC A 0 ppm A 0 ppm A 0 ppm A 0 ppm A 0 ppm A 0 ppm B 0 ppm B 0 ppm B 0 ppm B 0 ppm B 0 ppm B 0 ppm A 200 ppm A 200 ppm A 200 ppm A 200 ppm A 200 ppm A 200 ppm B 200 ppm B 200 ppm B 200 ppm B 200 ppm B 200 ppm B 200 ppm A 630 ppm A 630 ppm A 630 ppm A 630 ppm A 630 ppm A 630 ppm B 630 ppm B 630 ppm B 630 ppm B 630 ppm ABRCP 0.00 0.00 0.07 0.00 0.00 0.02 -0.05 0.02 -0.02 0.02 0.17 0.02 0.10 -0.02 0.00 -0.10 -0.05 0.10 0.05 0.02 -0.02 0.05 0.00 -0.07 0.02 0.05 0.05 0.00 -0.02 0.02 0.00 0.07 -0.02 0.00 ABRCC 0.984 0.972 0.940 0.934 0.960 0.893 0.949 0.930 0.961 0.932 0.836 0.960 0.940 0.974 0.794 0.923 0.926 0.943 0.941 0.991 0.963 0.934 0.971 0.658 0.968 0.975 0.970 0.974 0.965 0.962 0.957 0.931 0.950 0.645 ABRCPw 3.10 3.08 2.07 2.39 235 1.65 3.11 2.38 2.49 1.73 1.02 2.36 2.27 2.42 2-58 3.88 Z52 1.13 1.87 2.41 1.89 2.69 233 1.01 2.39 1.99 1.86 2.10 2.73 2.76 2.00 233 2.75 1.42 ID CONC ABRCP ABRCC ABRCPw Animal identification Exposure concentration ABR click 1.0-5.0 ms, latency difference from control template ABR click 1.0-5.0 ms, optimized cross-correlation value (r) ABR click 1.0-5.0 ms, power (RMS pV) SL 031029 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 2 of 3, Page: 193 Appendix K, continued: ABR click Data ID 90A3832 90A3833 90A3834 90A3837 90A3838 90A3839 90A3840 90A3841 90A3842 90A3843 90A3844 90A3845 90A3847 90A3848 90A3849 90A3850 90A3851 90A3852 90A3853 90A3855 90A3856 90A3857 90A3859 90A3860 90A3861 90A3862 90A3863 90A3864 90A3865 90A3867 90A3868 90A3869 90A3870 90A3872 SEX Male Male Male Male Male Male Male Male Male Male Male Male Male Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female GROUP CONC B 630 ppm B 630 ppm A 2000 ppm A 2000 ppm A 2000 ppm A 2000 ppm A 2000 ppm B 2000 ppm B 2000 ppm B 2000 ppm B 2000 ppm B 2000 ppm B 2000 ppm A 0 ppm A 0 ppm A 0 ppm A 0 ppm A 0 ppm A 0 ppm B 0 ppm B 0 ppm B 0 ppm B 0 ppm B 0 ppm B 0 ppm A 200 ppm A 200 ppm A 200 ppm A 200 ppm A 200 ppm A 200 ppm B 200 ppm B 200 ppm B 200 ppm ABRCP 0.00 0.07 0.05 -0.02 0.00 0.07 -0.05 -0.05 -0.02 0.12 0.00 0.05 0.14 0.05 0.07 0.00 -0.02 0.00 0.05 0.02 0.02 0.00 0.07 0.10 0.07 0.02 0.02 0.00 0.00 0.05 0.02 0.00 0.00 0.07 ABRCC 0.946 0.968 0.904 0.974 0.979 0.906 0.956 0.958 0.968 0.839 0.922 0.953 0.868 0.969 0.913 0.934 0.913 0.921 0.947 0.891 0.895 0.940 0.922 0.970 0.947 0.940 0.963 0.951 0.967 0.954 0.957 0.921 0.975 0.943 ABRCPw 2.68 2.73 2.31 2.11 3.24 1.66 2.64 3.12 2.67 1.54 2.69 1.54 1.13 199 140 157 3.28 3.56 183 1.74 1.44 3.36 104 136 183 3.01 134 3.04 169 177 3.05 146 3.19 173 ID CONC ABRCP ABRCC ABRCPw Animal identification Exposure concentration ABR click 1.0-5.0 ms, latency difference from control template ABR click 1.0*5.0 ms, optimized cross-correlation value (r) ABR click 1.0-5.0 ms, power (RMS 4V) SL 031030 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 2 of 3, Page: 194 Appendix K, continued: ABR click Data ID 90A3873 90A3874 90A3875 90A3876 90A3877 90A3878 90A3879 90A3880 90A3881 90A3883 90A3884 90A3885 90A3886 90A3887 90A3888 90A3890 90A3891 90A3892 90A3893 90A3895 90A3897 90A3898 90A3899 90A3900 90A3901 90A3902 90A3903 SEX Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female GROUP CONC B 200 ppm B 200 ppm B 200 ppm A 630 ppm A 630 ppm A 630 ppm A 630 ppm A 630 ppm A 630 ppm B 630 ppm B 630 ppm B 630 ppm B 630 ppm B 630 ppm B 630 ppm A 2000 ppm A 2000 ppm A 2000 ppm A 2000 ppm A 2000 ppm B 2000 ppm B 2000 ppm B 2000 ppm B 2000 ppm B 2000 ppm B 2000 ppm B 2000 ppm ABRCP 0.02 -0.02 0.10 0.07 0.02 -0.02 0.05 0.00 0.07 0.00 -0.02 0.07 0.05 -0.10 0.02 0.07 -0.02 -0.02 0.00 0.00 -0.02 0.02 0.02 0.12 0.05 0.14 0.00 ABRCC 0.965 0.921 0.900 0.884 0.934 0.948 0.942 0.908 0.893 0.946 0.950 0.907 0.967 0.617k 0.939 0.920 0.934 0.976 0.922 0.943 0.946 0.923 0.961 0.906 0.964 0.876 0.829 b Outlier by F-Max test. see Table 38. ID CONC ABRCP ABRCC ABRCPw Animal identification Exposure concentration ABR click 1.0-5.0 ms, latency difference from control template ABR click 1.0-5.0 ms, optimized cross-correlation value (r) ABR click 1.0-5.0 ms, power (RMS nV) ABRCPw 2.84 2.63 2.36 129 2.44 3.25 3.13 ZOO 1.82 3.43 2.60 1.76 3.10 2.28 2.87 2.29 3.27 3.54 3.00 2.82 3.54 3.07 2.84 1.78 2.42 1.61 1.77 03l31 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 2 of 3, Page: 195 Appendix L: ABRio tone Data ID 90A3792 90A3793 90A3795 90A3796 90A3797 90A3798 90A3799 90A3800 90A3802 90A3803 90A3804 90A3805 90A3806 90A3807 90A3808 90A3809 90A3810 90A3811 90A3813 90A3814 90A3815 90A3817 90A3818 90A3819 90A3820 90A3821 90A3822 90A3823 90A3824 90A3825 90A3828 90A3829 90A3830 SEX GROUP CONC Male A 0 ppm Male A 0 ppm Male A 0 ppm Male A 0 ppm Male A 0 ppm Male A 0 ppm Male B 0 ppm Male B 0 ppm Male B 0 ppm Male B 0 ppm Male B 0 ppm Male B 0 ppm Male A 200 ppm Male A 200 ppm Male A 200 ppm Male A 200 ppm Male A 200 ppm Male A 200 ppm Male B 200 ppm Male B 200 ppm Male B 200 ppm Male B 200 ppm Male B 200 ppm Male B 200 ppm Male A 630 ppm Male A 630 ppm Male A 630 ppm Male A 630 ppm Male A 630 ppm Male A 630 ppm Male B 630 ppm Male B 630 ppm Male B 630 ppm ABR10P 0.00 -0.02 0.12 0.05 -0.02 0.05 -0.05 -0.05 -0.05 0.05 0.26 -0.02 0.12 0.00 0.17 -0.07 -0.05 0.10 0.00 0.02 -0.07 -0.02 -0.02 -0.05 0.07 0.10 0.05 0.00 -0.02 0.05 0.00 - 0.14 -0.02 ABR10C 0.959 0.892 0.818 0.875 0.899 0.793 0.788 0.917 0.906 0.542 0.852 0.923 0.929 0.837 0.263 0.920 0.736 0.248b 0.494 0.649 0.789 0.917 0.705 0.326 0.852 0.725 0.640 0.914 0.883 0.873 0.842 0.921 0.931 ABRIOPw 0.72 0.80 0.56 0.57 0.60 0.46 0.81 0.75 0.52 0.43 0.38 0.91 0.71 0.78 0.60 0.82 0.67 0.44 0.44 0.58 0.47 0.85 0.74 0.28 0.71 0.61 0.53 0.63 0.75 0.60 0.58 0.76 0.56 b Outlier by F-Max test, see Table 38. ID CONC ABR10P ABR10C ABRIOPw Animal identification Exposure concentration ABR tone 10kHz 1.5-5.9 ms, latency difference from control template ABR tone 10kHz 13-5.9 ms, optimized cross-correlation value (r) ABR tone 10kHz 1.5-5.9 ms, power (RMS |iV) SL 031032 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 2 of 3, Page: 196 Appendix L, continued: ABRto tone Data ID 90A3831 90A3832 90A3833 90A3834 90A3837 90A3838 90A3839 90A3840 90A3841 90A3842 90A3843 90A3844 90A3845 90A3847 90A3848 90A3849 90A3850 90A3851 90A3852 90A3853 90A3855 90A3856 90A3857 90A3859 90A3860 90A3861 90A3862 90A3863 90A3864 90A3865 90A3867 90A3868 90A3869 90A3870 90A3872 SEX Male Male Male Male Male Male Male Male Male Male Male Male Male Male Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female GROUP CONC B 630 ppm B 630 ppm B 630 ppm A 2000 ppm A 2000 ppm A 2000 ppm A 2000 ppm A 2000 ppm B 2000 ppm B 2000 ppm B 2000 ppm B 2000 ppm B 2000 ppm B 2000 ppm A 0 ppm A 0 ppm A 0 ppm A 0 ppm A 0 ppm A 0 ppm B 0 ppm B 0 ppm B 0 ppm B 0 ppm B 0 ppm B 0 ppm A 200 ppm A 200 ppm A 200 ppm A 200 ppm A 200 ppm A 200 ppm B 200 ppm B 200 ppm B 200 ppm ABR10P -0.02 0.05 0.14 0.05 -0.07 0.05 0.14 -0.12 -0.12 -0.05 0.14 -0.05 0.00 0.12 0.05 -0.05 -0.05 -0.07 -0.02 0.05 0.02 0.00 -0.02 0.05 0.10 0.05 -0.02 -0.02 -0.02 -0.07 0.05 - 0.02 -0.07 -0.05 0.05 ABR10C 0.807 0.869 0.807 0.903 0.921 0.845 0.788 0.879 0.898 0.833 0.715 0.925 0.684 0.223 0.620 0.708 0.732 0.930 0.919 0.896 0.920 0.843 0.925 0.761 0.904 0.776 0.867 0.947 0.823 0.637 0.793 0.976 0.741 0.867 0.635 ABRIOPw 0.36 0.79 0.66 0.69 0.62 0.73 0.50 0.50 0.64 0.72 0.80 0.67 0.66 0.43 0.89 0.88 0.51 1.12 0.88 0.77 0.52 0.63 0.98 0.52 0.83 0.66 0.71 0.75 0.78 0.66 0.73 0.85 0.81 0.83 056 ID CONC ABR10P ABR10C ABRIOPw Animal identification Exposure concentration ABR tone 10kHz 1.5-5.9 ms, latency difference from control template ABR tone 10kHz 15-5.9 ms, optimized cross-correlation value (r) ABR tone 10kHz 1.5-5.9 ms, power (RMS pV) SL 031033 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 2 of 3, Page: 197 Appendix L, continued: ABRio tone Data ID 90A3873 90A3874 90A3875 90A3876 90A3877 90A3878 90A3879 90A3880 90A3881 90A3883 90A3884 90A3885 90A3886 90A3887 90A3888 90A3890 90A3891 90A3892 90A3893 90A3895 90A3897 90A3898 90A3899 90A3900 90A3901 90A3902 90A3903 SEX GROUP CONC Female B 200 ppm Female B 200 ppm Female B 200 ppm Female A 630 ppm Female A 630 ppm Female A 630 ppm Female A 630 ppm Female A 630 ppm Female A 630 ppm Female B 630 ppm Female B 630 ppm Female B 630 ppm Female B 630 ppm Female B 630 ppm Female B 630 ppm Female A 2000 ppm Female A 2000 ppm Female A 2000 ppm Female A 2000 ppm Female . A 2000 ppm Female B 2000 ppm Female B 2000 ppm Female B 2000 ppm Female B 2000 ppm Female B 2000 ppm Female B 2000 ppm Female B 2000 ppm ABR10P -0.05 0.00 0.05 0.10 -0.05 -0.05 0.07 -0.02 0.02 0.02 -0.07 0.14 0.00 -0.17 0.00 0.10 -0.07 -0.02 -0.10 -0.05 -0.10 -0.10 -0.05 0.19 0.00 0.19 0.10 ABR10C 0.796 0.852 0.780 0.771 0.773 0.840 0.867 0.960 0.861 0.881 0.727 0.718 0.934 0.755 0.770 0.864 0.728 0.940 0.930 0.912 0.810 0.879 0.867 0.801 0.923 0.724 0.427 ABRIOPw 0.81 0.62 0.90 0.61 0.82 0.92 0.80 0.70 0.67 0.88 0.81 0.60 0.73 0.82 0.80 0.89 0.83 0.94 1.07 0.84 0.98 1.14 0.94 0.52 0.86 0.55 0.31 ID CONC ABR10P ABR10C ABRIOPw Animal identification Exposure concentration ABR tone 10kHz 1.5-5.9 ms, latency difference from control template ABR tone 10kHz 1.5-5.9 ms, optimized cross-correlation value (r) ABR tone 10kHz 1.5-5.9 ms, power (RMS p,V) SL 031034 1,1.1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 2 of 3, Page: 198 Appendix M: ABR30 tone Data ID 90A3792 90A3793 90A3795 90A3796 90A3797 90A3798 90A3799 90A3800 90A3802 90A3803 90A3804 90A3805 90A3806 90A3807 90A3808 90A3809 90A3810 90A3811 90A3813 90A3814 90A3815 90A3817 90A3818 90A3819 90A3820 90A3821 90A3822 90A3823 90A3824 90A3825 90A3828 90A3829 90A3830 90A3831 SEX Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male GROUP CONC A 0 ppm A 0 ppm A 0 ppm A 0 ppm A 0 ppm A 0 ppm B 0 ppm B 0 ppm B 0 ppm B 0 ppm B 0 ppm B 0 ppm A 200 ppm A 200 ppm A 200 ppm A 200 ppm A 200 ppm A 200 ppm B 200 ppm B 200 ppm B 200 ppm B 200 ppm B 200 ppm B 200 ppm A 630 ppm A 630 ppm A 630 ppm A 630 ppm A 630 ppm A 630 ppm B 630 ppm B 630 ppm B 630 ppm B 630 ppm ABR30P *0.10 0.00 0.02 0.00 0.02 -0.10 -0.07 0.07 -0.02 0.00 0.17 0.19 0.22 -0.05 0.05 -0.05 -0.14 0.14 0.05 0.05 -0.02 0.05 -0.17 -0.14 0.00 0.05 0.00 0.12 -0.07 -0.07 -0.02 - 0.17 -0.02 -0.02 ABR30C 0.909 0.703 0.779 0.757 0.820 0.018 0.643 0.641 0.859 0.411 0.598 0.612 0.797 0.851 0.887 0.842 0.777 0.595 0.447 0.746 0.812 0.793 0.771 0.592 0.881 0.692 0.630 0.797 0.843 0.811 0.918 0.663 0.769 0.481 ABR30Pw 0.61 0.53 0.43 0.42 0.46 0.34 0.78 0.54 0.22 0.37 0.41 0.51 0.41 0.50 0.45 1.00 0.58 0.67 039 0.52 0.38 0.58 0.84 0.44 0.41 0.67 0.42 0.60 0.66 050 0.49 051 0.70 055 ID CONC ABR30P ABR30C ABR30Pw Animal identification Exposure concentration ABR tone 30kHz 1.5-5.5 ms, latency difference from control template ABR tone 30kHz 1.5-5.5 ms, optimized cross-correlation value (r) ABR tone 30kHz 1.5-5.5 ms, power (RMS |iV) SL 031035 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 2 of 3, Page: 199 Appendix M, continued: ABR30 tone Data ID 90A3832 90A3833 90A3834 90A3837 90A3838 90A3839 90A3840 90A3841 90A3842 90A3843 90A3844 90A3845 90A3847 90A3848 90A3849 90A3850 90A3851 90A3852 90A3853 90A3855 90A3856 90A3857 90A3859 90A3860 90A3861 90A3862 90A3863 90A3864 90A3865 90A3867 90A3868 90A3869 90A3870 90A3872 SEX Male Male Male Male Male Male Male Male Male Male Male Male Male Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female GROUP CONC B 630 ppm B 630 ppm A 2000 ppm A 2000 ppm A 2000 ppm A 2000 ppm A 2000 ppm B 2000 ppm B 2000 ppm B 2000 ppm B 2000 ppm B 2000 ppm B 2000 ppm A 0 ppm A 0 ppm A 0 ppm A 0 ppm A 0 ppm A 0 ppm B 0 ppm B 0 ppm B 0 ppm B 0 ppm B 0 ppm B 0 ppm A 200 ppm A 200 ppm A 200 ppm A 200 ppm A 200 ppm A 200 ppm B 200 ppm B 200 ppm B 200 ppm ABR30P -0.05 0.07 0.07 0.10 -0.02 0.43 -0.17 -0.10 0.00 0.29 0.05 0.05 0.26 0.05 0.07 -0.02 -0.02 0.00 0.05 -0.02 0.05 0.00 0.12 0.17 0.10 0.05 0.10 0.10 -0.07 0.07 0.19 0.10 0.02 0.19 ABR30C 0.832 0.841 0.777 0.872 0.853 0.560 0.791 0.882 0.894 0.598 0.884 0.719 0.580 0.774 0.871 0.864 0.800 0.907 0.774 0.784 0.578 0.942 0.793 0.546 0.495 0.573 0.761 0.855 0.911 0.904 0.765 0.551 0.882 0.787 ABR3 0.66 0.67 0.42 050 0.62 0.44 0.69 0.43 0.69 0.49 0.26 0.62 0.32 0.74 0.45 0.96 0.65 0.74 0.53 0.48 038 0.80 0.46 0.47 0.39 0.59 0.43 0.92 058 0.66 0.96 0.44 057 059 ID CONC ABR30P ABR30C ABR30Pw Animal identification Exposure concentration ABR tone 30kHz 1.5-5.5 ms, latency difference from control template ABR tone 30kHz 1.5-5.5 ms, optimized cross-correlation value (r) ABR tone 30kHz 1.5-5.5 ms, power (RMS uV) SL 031036 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 2 of 3, Page: 200 Appendix M, continued: ABR30 tone Data ID 90A3873 90A3874 90A3875 90A3876 90A3877 90A3878 90A3879 90A3880 90A3881 90A3883 90A3884 90A3885 90A3886 90A3887 90A3888 90A3890 90A3891 90A3892 90A3893 90A3895 90A3897 90A3898 90A3899 90A3900 90A3901 90A3902 90A3903 SEX GROUP CONC Femaie B 200 ppm Female B 200 ppm Female B 200 ppm Femaie A 630 ppm Female A 630 ppm Female A 630 ppm Female A 630 ppm Female A 630 ppm Female A 630 ppm Female B 630 ppm Female B 630 ppm Female B 630 ppm Female B 630 ppm Female B 630 ppm Female B 630 ppm Female A 2000 ppm Female A 2000 ppm Female A 2000 ppm Female A 2000 ppm Female A 2000 ppm Femaie B 2000 ppm Female B 2000 ppm Female B 2000 ppm Femaie B 2000 ppm Female B 2000 ppm Female B 2000 ppm Female B 2000 ppm ABR30P 0.10 -0.07 0.29 0.19 0.07 -0.05 0.02 -0.07 0.24 0.10 -0.07 0.14 0.02 -0.14 0.02 0.26 0.02 0.02 0.10 -0.10 0.00 0.07 0.07 0.12 0.00 0.26 -0.02 ABR30C 0.861 0.887 0.552 0.602 0.707 0.894 0.716 0.664 0.668 0.798 0.755 0.566 0.842 0.496 0.800 0.748 0.840 0.889 0.895 0.910 0.777 0.761 0.830 0.665 0.866 0.363 0.891 ABR30Pw 0.46 0.87 0.67 0.44 0.81 0.82 0.57 0.62 0.50 0.97 0.48 0.36 0.49 0.68 0.77 0.53 0.75 0.74 053 0.76 0.82 0.69 0.90 0.70 051 0.42 0.44 ID CONC ABR30P A8R30C ABR30Pw Animal identification Exposure concentration ABR tone 30kHz 1.5-5.5 ms, latency difference from control template ABR tone 30kHz 1.5-5.5 ms, optimized cross-correlation value (r) ABR tone 30kHz 1.5-5.5 ms, power (RMS p-V) SL 03X037 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 2 of 3, Page: 201 Appendix N: CNAP Data ID 90A3792 90A3793 90A3795 90A3796 90A3797 90A3798 90A3799 90A3800 90A3802 90A3803 90A3804 90A3805 90A3806 90A3807 90A3808 90A3809 90A3810 90A3811 90A3813 90A3814 90A3815 90A3817 90A3818 90A3819 90A3820 90A3821 90A3822 90A3823 90A3824 90A3825 90A3828 90A3829 90A3830 90A3831 SEX Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male GROUP CONC A 0 ppm A 0 ppm A 0 ppm A 0 ppm A 0 ppm A 0 ppm B 0 ppm B 0 ppm B 0 ppm B 0 ppm B 0 ppm B 0 ppm A 200 ppm A 200 ppm A 200 ppm A 200 ppm A 200 ppm A 200 ppm B 200 ppm B 200 ppm B 200 ppm B 200 ppm B 200 ppm B 200 ppm A 630 ppm A 630 ppm A 630 ppm A 630 ppm A 630 ppm A 630 ppm B 630 ppm B 630 ppm B 630 ppm B 630 ppm CNAPSP 0.04 0.00 -0.44 0.36 0.12 0.20 0.72 -0.08 -0.08 -0.28 0.16 0.28 -0.08 -0.08 -0.04 0.16 0.16 -0.16 -0.08 -0.28 -0.20 0.08 -0.28 -0.20 0.12 0.32 0.28 0.00 0.32 -0.16 0.72 -0.12 0.12 0.12 CNAPSC 0.948 0.964 0.871 0.959 0.975 0.972 0.852 0.949 0.983 0.933 0.951 0.949 0.987 0.930 0.970 0.916 0.873 0.931 0.984 0.940 0.977 0.947 0.962 0.954 0.981 0.930 0.969 0.981 0.864 0.976 0.912 0.983 0.993 0.933 CNAPSPw 15.59 10.95 16.14 11.88 15.81 8.96 1056 20.94 27.19 13.93 20.66 11.45 11.64 20.13 7.25 15.11 14.77 15.02 17.69 19.21 19.36 18.99 22.82 19.28 8.16 7.44 11.81 1455 12.13 14.86 6.06 16.17 19.34 1055 ID CONC CNAPSP CNAPSC CNAPSPw Animal identification Exposure concentration CNAP single pulse 2.S-7.4 ms, latency difference from control template CNAP single pulse 2.8-7.4 ms, optimized cross-correlation value (r) CNAP single pulse 25-7.4 ms, power (RMS p.V) SL 031038 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 2 of 3, Page: 202 Appendix N, continued: CNAP Data ID 90A3832 90A3833 90A3834 90A3837 90A3838 90A3839 90A3840 90A3841 90A3842 90A3843 90A3844 90A3845 90A3847 90A3848 90A3849 90A3850 90A3851 90A3852 90A3853 90A3855 90A3856 90A3857 90A3859 90A3860 90A3861 90A3862 90A3863 90A3864 90A3865 90A3867 90A3868 90A3869 90A3870 90A3872 SEX Male Male Male Male Male Male Male Male Male Male Male Male Male Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female GROUP CONC B 630 ppm B 630 ppm A 2000 ppm A 2000 ppm A 2000 ppm A 2000 ppm A 2000 ppm B 2000 ppm B 2000 ppm B 2000 ppm B 2000 ppm B 2000 ppm B 2000 ppm A 0 ppm A 0 ppm A 0 ppm A 0 ppm A 0 ppm A 0 ppm B 0 ppm B 0 ppm B 0 ppm B 0 ppm B 0 ppm B 0 ppm A 200 ppm A 200 ppm A 200 ppm A 200 ppm A 200 ppm A 200 ppm B 200 ppm B 200 ppm B 200 ppm CNAPSP -0.04 -0.20 -0.12 0.16 0.00 -0.12 0.16 0.04 -0.04 0.04 0.12 0.84 -0.36 0.40 0.16 -0.04 -0.12 -0.16 0.04 0.12 0.00 -0.12 -0.12 0.12 -0.04 0.32 0.28 0.76 -0.16 0.04 0.16 '0.28 0.00 -0.32 CNAPSC 0.953 0.968 0.967 0.945 0.937 0.948 0.892 0.961 0.986 0.951 0.937 0.960 0.905 0.955 0.987 0.989 0.987 0.918 0.988 0.991 0.989 0.991 0.947 0.995 0.974 0.976 0.988 0.895 0.994 0.930 0.750 0.928 0.988 0.886 CNAPSPw 20.69 12.09 10.91 11.24 12.48 12.19 18.79 13.88 17.70 10.89 12.70 5.96 17.61 14.22 1732 21.02 2533 1139 17.16 1935 17.84 21.16 1938 16.12 11.66 18.96 17.08 10.28 23.43 15.72 8.49 19.77 1836 18.61 ID CONC CNAPSP CNAPSC CNAPSPw Animal identification Exposure concentration CNAP single pulse 2.8-7.4 ms, latency difference from control template CNAP single pulse 2.8-7.4 ms, optimized cross-correlation value (r) CNAP single pulse 23-7.4 ms, power (RMS p,V) SL 031039 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 2 of 3, Page: 203 Appendix N, continued: CNAP Data ID 90A3873 90A3874 90A3875 90A3876 90A3877 90A3878 90A3879 90A3880 90A3881 90A3883 90A3884 90A3885 90A3886 90A3887 90A3888 90A3890 90A3891 90A3892 90A3893 90A3895 90A3897 90A3898 90A3899 90A3900 90A3901 90A3902 90A3903 SEX Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female GROUP CONC B 200 ppm B 200 ppm B 200 ppm A 630 ppm A 630 ppm A 630 ppm A 630 ppm A 630 ppm A 630 ppm B 630 ppm B 630 ppm B 630 ppm B 630 ppm B 630 ppm B 630 ppm A 2000 ppm A 2000 ppm A 2000 ppm A 2000 ppm A 2000 ppm B 2000 ppm B 2000 ppm B 2000 ppm B 2000 ppm B 2000 ppm B 2000 ppm B 2000 ppm CNAPSP 0.12 0.28 0.36 0.28 -0.16 0.08 -0.28 0.40 0.32 0.00 -0.08 0.08 0.04 -0.24 -0.12 -0.20 0.08 0.04 0.00 -0.20 -0.24 0.04 -0.08 0.16 -0.16 0.24 0.08 CNAPSC 0.950 0.972 0.959 0.980 0.982 0.979 0.983 0.906 0.944 0.972 0.961 0.975 0.967 0.977 0.976 0.926 0.980 0.965 0.873 0.964 0.940 0.989 0.887 0.952 0.970 0.984 0.961 CNAPSPw 16.61 22.21 14.38 13.68 21.39 21.88 18.82 1X00 6.81 2534 27.63 20.00 19.08 27.41 25.85 23.60 1534 17.96 3.14 28.02 21.25 10.95 3139 19.34 2X95 13.31 6.19 ID CONC CNAPSP CNAPSC CNAPSPw Animal identification Exposure concentration CNAP single pulse 2.8-7.4 ms, latency difference from control template CNAP single pulse 2.8-7.4 ms, optimized cross-correlation value (r) CNAP single pulse 2&-7A ms, power (RMS p.V) SL 031040 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 2 of 3, Page: 204 Appendix N, continued: CNAP Data ID 90A3792 90A3793 90A3795 90A3796 90A3797 90A3798 90A3799 90A3800 90A3802 90A3803 90A3804 90A3805 90A3806 90A3807 90A3808 90A3809 90A3810 90A3811 90A3813 90A3814 90A3815 90A3817 90A3818 90A3819 90A3820 90A3821 90A3822 90A3823 90A3824 90A3825 90A3828 90A3829 90A3830 SEX Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male GROUP CONC A 0 ppm A 0 ppm A 0 ppm A 0 ppm A 0 ppm A 0 ppm B 0 ppm B 0 ppm B 0 ppm B 0 ppm B 0 ppm B 0 ppm A 200 ppm A 200 ppm A 200 ppm A 200 ppm A 200 ppm A 200 ppm B 200 ppm B 200 ppm B 200 ppm B 200 ppm B 200 ppm B 200 ppm A 630 ppm A 630 ppm A 630 ppm A 630 ppm A 630 ppm A 630 ppm B 630 ppm B 630 ppm B 630 ppm CNAPPP 0.00 -0.08 -0.40 0.48 0.16 0.68 0.92 0.04 -0.04 -0.32 0.12 0.40 -0.16 -0.08 -0.08 0.36 0.16 -0.12 -0.12 -0.24 0.04 0.20 -0.28 -0.16 0.20 032 0.28 -0.12 0.40 -0.24 0.00 ' 0.04 0.12 CNAPPC 0.894 0.940 0.940 0.978 0.965 0.945 0.917 0.971 0.960 0.982 0.889 0.858 0.965 0.868 0.958 0.945 0.909 0.919 0.967 0.956 0.954 0.906 0.930 0.931 0.987 0.982 0.948 0.960 0.643b 0.894 0.202 0.976 0.980 CNAPPPw 1190 8.75 14.50 8.50 1335 4.11 8.28 1531 21.26 13.81 14.29 7.82 10.43 15.28 5.32 1130 1138 1033 15.22 16.80 10.79 1142 21.68 15.05 6.60 6.97 10.03 1160 8.12 1138 189 11.96 13.83 b Outlier by F-Max test, see Table 38. ID CONC CNAPPP CNAPPC CNAPPPw Animal identification Exposure concentration CNAP paired pulse 6.0-10.0 ms, latency difference from control template CNAP paired pulse 6.0-10.0 ms, optimized cross-correlation value (r) CNAP paired pulse 6.0-10.0 ms, power (RMS pV) 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 2 of 3, Page: 205 Appendix N, continued: CNAP Data ID 90A3831 90A3832 90A3833 90A3834 90A3837 90A3838 90A3839 90A3840 90A3841 90A3842 90A3843 90A3844 90A3845 90A3847 90A3848 90A3849 90A3850 90A3851 90A3852 90A3853 90A3855 90A3856 90A3857 90A3859 90A3860 90A3861 90A3862 90A3863 90A3864 90A3865 90A3867 90A3868 90A3869 SEX Male Male Male Male Male Male Male Male Male Male Male Male Male Male Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female GROUP CONC B 630 ppm B 630 ppm B 630 ppm A 2000 ppm A 2000 ppm A 2000 ppm A 2000 ppm A 2000 ppm B 2000 ppm B 2000 ppm B 2000 ppm B 2000 ppm B 2000 ppm B 2000 ppm A 0 ppm A 0 ppm A 0 ppm A 0 ppm A 0 ppm A 0 ppm B Oppm B 0 ppm B 0 ppm B 0 ppm B 0 ppm B 0 ppm A 200 ppm A 200 ppm A 200 ppm A 200 ppm A 200 ppm A 200 ppm B 200 ppm CNAPPP 0.32 -0.12 -0.12 -0.12 0.20 0.08 -0.08 0.20 0.12 -0.04 0.04 0.24 0.20 -0.40 0.52 0.24 -0.08 -0.12 -0.16 0.04 0.08 -0.04 -0.08 -0.08 0.20 -0.04 0.36 0.28 0.88 -0.16 0.04 0.08 -0.32 CNAPPC 0.845 0.956 0.952 0.950 0.948 0.948 0.923 0.872 0.859 0.987 0,956 0.700 0.172b 0.907 0.896 0.991 0.979 0.986 0.979 0.986 0.979 0.946 0.978 0.938 0.964 0.869 0.960 0.979 0.863 0.970 0.983 0.878 0.904 CNAPPPw 5.60 19.23 8.50 9.18 7.49 1039 10.27 16.60 9.68 12.65 6.14 7.39 2.54 13.93 7.75 13.66 14.00 21.74 10.70 13.03 1430 8.60 19.42 15.17 11.64 5.63 14.70 1230 630 18.96 1Z11 6.08 15.96 b Outlier by F-Max test, see Table 38. ID CONC CNAPPP CNAPPC CNAPPPw Animal identification Exposure concentration CNAP paired pulse 6.0-10.0 ms, latency difference from control template CNAP paired pulse 6.0-10.0 ms, optimized cross-correlation value (r) CNAP paired pulse 6.0-10.0 ms, power (RMS |i.V) SL 031042 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 2 of 3, Page: 206 Appendix N, continued: CNAP Data ID 90A3870 90A3872 90A3873 90A3874 90A3875 90A3876 90A3877 90A3878 90A3879 90A3880 90A3881 90A3883 90A3884 90A3885 90A3886 90A3887 90A3888 90A3890 90A3891 90A3892 90A3893 90A3895 90A3897 90A3898 90A3899 90A3900 90A3901 90A3902 90A3903 SEX Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female GROUP CONC B 200 ppm B 200 ppm B 200 ppm B 200 ppm B 200 ppm A 630 ppm A 630 ppm A 630 ppm A 630 ppm A 630 ppm A 630 ppm B 630 ppm B 630 ppm B 630 ppm B 630 ppm B 630 ppm B 630 ppm A 2000 ppm A 2000 ppm A 2000 ppm A 2000 ppm A 2000 ppm B 2000 ppm B 2000 ppm B 2000 ppm B 2000 ppm B 2000 ppm B 2000 ppm B 2000 ppm CNAPPP 0.00 -0.24 0.16 0.28 0.28 0.32 -0.16 0.12 -0.28 0.36 0.52 0.00 -0.04 0.16 0.04 -0.28 -0.08 -0.16 0.04 0.08 0.00 -0.16 -0.28 0.00 -0.08 0.20 -0.08 0.32 0.40 CNAPPC 0.992 0.929 0.919 0.906 0.965 0.969 0.932 0.974 0.978 0.949 0.758 0.942 0.944 0.995 0.981 0.983 0.948 0.907 0.981 0.983 0.589b 0.989 0.969 0.928 0.897 0.880 0.963 0.868 0.682 CNAPPPw 14.65 15.83 13.26 14.09 8.20 1032 18.97 15.88 13.25 7.85 2.88 20.22 20.32 16.33 14.78 25.07 20.61 17.93 12.16 14.60 2.85 25.20 15.86 7.55 21.16 1333 17.06 6.85 1.98 b Outlier by F-Max test, see Table 38. ID CONC CNAPPP CNAPPC CNAPPPw Animal identification Exposure concentration CNAP paired pulse 6.0-10.0 ms, latency difference from control template CNAP paired pulse 6.0-10.0 ms, optimized cross-correlation value (r) CNAP paired pulse 6.0-10.0 ms, power (RMS jiV) SL 0310A3 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 2 of 3, Page: 207 Appendix O: CFR Data ID 90A3792 90A3793 90A3795 90A3796 90A3797 90A3798 90A3799 90A3800 90A3802 90A3803 90A3804 90A3805 90A3806 90A3807 90A3808 90A3809 90A3810 90A3811 90A3813 90A3814 90A3815 90A3817 90A3818 90A3819 90A3820 90A3821 90A3822 90A3823 90A3824 90A3825 90A3828 90A3829 90A3830 90A3831 SEX Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male GROUP CONC A 0 ppm A 0 ppm A 0 ppm A 0 ppm A 0 ppm A 0 ppm B 0 ppm B 0 ppm B 0 ppm B 0 ppm B 0 ppm B 0 ppm A 200 ppm A 200 ppm A 200 ppm A 200 ppm A 200 ppm A 200 ppm B 200 ppm B 200 ppm B 200 ppm B 200 ppm B 200 ppm B 200 ppm A 630 ppm A 630 ppm A 630 ppm A 630 ppm A 630 ppm A 630 ppm B 630 ppm B 630 ppm B 630 ppm B 630 ppm ID CONC CFRS CFRN CFRR Animal identification Exposure concentration CFR signal CFR noise CFR signal to noise ratio CFRS 5.41 2.81 3.31 3.33 4.56 331 2.71 3.33 2.76 3.93 3.87 4.30 3.28 2.81 2.95 2.95 3.13 3.37 2.40 2.38 2.89 3.46 2.55 3.54 3.79 3.75 3.25 3.12 330 231 3.01 230 3.62 2.23 CFRN 3.33 2.29 231 2.14 3.94 3.20 3.37 3.32 231 3.63 4.76 2.74 232 3.61 3.00 2.02 2.17 4.20 2.93 2.98 6.28 3.62 3.64 3.09 2.84 1.89 3.91 3.97 1.81 3.36 1.91 439 3.96 3.71 CFRR 2.64 1.51 2.05 2.42 1.34 1.07 0.65 1.01 1.21 1.17 0.66 2.46 1.69 0.61 0.97 113 2.08 0.64 0.67 0.64 031 0.91 0.49 131 1.78 3.94 0.69 0.62 332 0.47 2.48 032 0.84 0.36 SL 031044 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 2 of 3, Page: 208 Appendix O, continued: CFR Data ID 90A3832 90A3833 90A3834 90A3837 90A3838 90A3839 90A3840 90A3841 90A3842 90A3843 90A3844 90A3845 90A3847 90A3848 90A3849 90A3850 90A3851 90A3852 90A3853 90A3855 90A3856 90A3857 90A38S9 90A3860 90A3861 90A3862 90A3863 90A3864 90A3865 90A3867 90A3868 90A3869 90A3870 90A3872 Six Male Male Male Male Male Male Male Male Male Male Male Male Male Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female GROUP CONC B 630 ppm B 630 ppm A 2000 ppm A 2000 ppm A 2000 ppm A 2000 ppm A 2000 ppm B 2000 ppm B 2000 ppm B 2000 ppm B 2000 ppm B 2000 ppm B 2000 ppm A 0 ppm A 0 ppm A 0 ppm A 0 ppm A 0 ppm A 0 ppm B 0 ppm B 0-ppm B 0 ppm B 0 ppm B 0 ppm B 0 ppm A 200 ppm A 200 ppm A 200 ppm A 200 ppm A 200 ppm A 200 ppm B 200 ppm B 200 ppm B 200 ppm ID CONC CFRS CFRN CFRR Animal identification Exposure concentration CFR signal CFR noise CFR signal to noise ratio CFRS 3.51 2.42 4.19 3.07 3.21 3.28 5.27 4.67 3.46 3.37 4.99 2.97 3.78 3.28 4.17 2.35 4.94 2.67 2.78 2.93 3.66 5.96 4.24 3.31 3.76 5.06 2.78 4.10 1.96 3.51 4.64 - 2.78 4.83 4.32 CFRN 5.27 4.10 2.42 4.80 3.55 4.27 4.70 3.37 1.92 2.54 3.12 2.79 4.13 4.10 5.21 4.63 3.05 3.02 3.64 3.34 3.39 2.62 2.87 1.85 3.73 2.08 3.62 2.77 3.83 4.17 4.12 7.95 1.88 3.51 CFRR 0.44 0.35 3.00 0.41 0.82 0.59 1.26 1.92 3.25 1.76 2.56 1.13 0.84 0.64 0.64 0.26 2.62 0.78 0.58 0.77 1.17 5.17 2.18 3.20 1.02 5.92 0259 Z19 0.26 0.71 1.27 0.12 6.60 151 1*5 0^ 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 2 of 3, Page: 209 Appendix O, continued: CFR Data ID 90A3873 90A3874 90A3875 90A3876 90A3877 90A3878 90A3879 90A3880 90A3881 90A3883 90A3884 90A3885 90A3886 90A3887 90A3888 90A3890 90A3891 90A3892 90A3893 90A3895 90A3897 90A3898 90A3899 90A3900 90A3901 90A3902 90A3903 SEX Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female GROUP CONC B 200 ppm B 200 ppm B 200 ppm A 630 ppm A 630 ppm A 630 ppm A 630 ppm A 630 ppm A 630 ppm B 630 ppm B 630 ppm B 630 ppm B 630 ppm B 630 ppm B 630 ppm A 2000 ppm A 2000 ppm A 2000 ppm A 2000 ppm A 2000 ppm B 2000 ppm B 2000 ppm B 2000 ppm B 2000 ppm B 2000 ppm B 2000 ppm B 2000 ppm ID CONC CFRS CFRN CFRR Animal identification Exposure concentration CFR signal CFR noise CFR signal to noise ratio CFRS 7.28 3.67 5.09 2.82 3.82 4.91 230 2.73 4.13 3.88 2.73 2.65 4.33 3.35 5.10 1131 184 236 4.03 1.91 5.48 3.64 239 3.33 2.60 4.66 3.03 CFRN 4.87 2.43 3.32 4.35 2.87 4.04 2.75 133 3.79 3.48 7.78 3.82 1.82 2.91 3.88 4.41 3.71 1.56 4.51 2.07 530 3.31 3.02 5.57 2.45 4.60 3.03 CFRR 223 228 235 0.42 1.77 1.48 0.70 3.18 1.19 1.24 0.12 0.48 5.66 1.33 1.73 6.81 039 269 0.80 0.85 0.99 1.21 0.74 0.36 1.13 1.03 1.00 SL 031046 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 3 of 3, Page: 1 Appendix P: Brain Weight Data ID 90A3793 90A3796 90A3798 90A3804 90A3805 90A3807 90A3811 90A3813 90A3814 90A3819 90A3821 90A3825 90A3826 90A3830 90A3833 90A3837 90A3838 90A3841 90A3844 90A3845 SEX Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male CONC ckqut 0 ppm 0 ppm 0 ppm 0 ppm 0 ppm 200 ppm 200 ppm 200 ppm 200 ppm 200 ppm 630 ppm 630 ppm 630 ppm 630 ppm 630 ppm 2000 ppm 2000 ppm 2000 ppm 2000 ppm 2000 ppm A A A B B A A B B B A A A B B A A B B B ID Animal identification CONC Exposure concentration GROUP Group B started one week after Group A. BWTNEC Body weight at necropsy BRNWT Brain weight BWTNEC 301.8 268.6 307.4 319.7 294.2 308.9 347.0 311.6 293.6 308.0 290.6 300.8 304.3 261.0 325.7 345.1 290.4 328.5 331.7 306.7 5BNWI 1.773 1.801 1.917 1.937 1.902 1.787 1.854 1.862 1.821 1.783 1.775 1.805 1.858 1.709 1.896 1.873 1.925 1.809 1-853 1.928 SL 031047 1,1,1-T subchronic neurotoxicity The Dow Chemical Company Project ID: K-001716-091 Volume 3 of 3, Page: 2 Appendix P, continued: Brain Weight Data _JD 90A3852 90A3854 90A3855 90A3856 90A3857 90A3862 90A3864 90A3865 90A3869 90A3875 90A3877 90A3882 90A3883 90A3885 90A3886 90A3891 90A3892 90A3893 90A3897 90A3901 SEX Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female Female CQN.C GROUP 0 ppm 0 ppm 0 ppm 0 ppm 0 ppm 200 ppm 200 ppm 200 ppm 200 ppm 200 ppm 630 ppm 630 ppm 630 ppm 630 ppm 630 ppm 2000 ppm 2000 ppm 2000 ppm 2000 ppm 2000 ppm A A B B B A A A B B A A B B B A A A B B ID Animal identification CONC Exposure concentration GROUP Group B started one week after Group A. BWTNEC Body weight at necropsy BRNWT Brain weight BWTNEC 174.9 174.6 175.8 174.6 169.8 172.7 166.6 163.4 171.5 177.4 184.6 180.5 161.0 163.1 158.3 173.2 174.8 177.3 169.0 172.1 BRNWT 1.610 1.719 1.721 1.688 1.708 1.714 1.658 1.664 1.757 1.725 1.655 1.697 1.723 1.684 1.665 1.680 1.697 1.782 1.697 1.701 SL 031048 1,1,1-T subchronic neurotoxicity APPENDIX Q: GROSS PATHOLOGY, GROUP A The Dow Chemical Company Study ID: K-001716-091 Volume 3 of 3, Page: 3 ANIMAL NUMBER: 90A3792 SEX: MALE DOSE: 0 FINAL BODY HEIGHT (GRAMS): 319.3 PPM TEST MATERIAL: MODE OF DEATH: NECROPSY DATE: 1,1,1-T SACRIFICED 10/09/90 (DAY 134) GROSS PATHOLOGIC OBSERVATIONS (COMPLETE NECROPSY PERFORMED): ALL TISSUES HERE EXAMINED AND HERE HITHIN NORMAL LIMITS * I END OF REPORT FOR ANIMAL 90A3792 I DURATION: ROUTE: SPECIES: 19-WEEK INHALATION RAT SL 031049 ANIMAL NUMBER; 90A3793 SEX; MALE ' DOSE: 0 FINAL BODY HEIGHT (GRAMS): 301.6 PPM TEST MATERIAL: MODE OF DEATH: NECROPSY DATE: 1,1,1-T SACRIFICED 09/06/90 (DAY 101) ORGAN HEIGHTS (GRAMS): BRAIN 1.773 GROSS PATHOLOGIC OBSERVATIONS (COMPLETE NECROPSY PERFORMED): ALL TISSUES HERE EXAMINED AND HERE WITHIN NORMAL LIMITS* I END OF REPORT FOR ANIMAL 90A3793 t DURATION; ROUTE; SPECIES: 19-WEEK INHALATION RAT 1,1,1-T subchronic neurotoxicity APPENDIX Q: GROSS PATHOLOGY, GROUP A The Dow Chemical Company Study ID: K-001716-091 Volume 3 of 3, Page: 4 ANIMAL NUMBER: 90A3794 SEX: MALE DOSE; 0 PPM TEST MATERIAL: MODE OF DEATH: NECROPSY DATE: 1,1,1-T SPONTANEOUS 07/31/90 (DAY 64) DURATION: ROUTE: SPECIES: 19-WEEK INHALATION RAT GROSS PATHOLOGIC OBSERVATIONS (COMPLETE NECROPSY PERFORMED): GENERAL CONGESTION, VISCERA W GENERAL COMMENT CAUSE OF DEATH HAS NOT DETERMINED. ALL OTHER TISSUES HERE WITHIN NORMAL LIMITS I END OF REPORT FOR ANIMAL 90A3794 I 03105 ANIMAL NUMBER: 90A3795 SEX: MALE DOSE: 0 FINAL BODY WEIGHT (GRAMS): 325.7 PPM TEST MATERIAL: MODE OF DEATH: NECROPSY DATE: 1, 1,1-T SACRIFICED 10/09/90 (DAY 134) GROSS PATHOLOGIC OBSERVATIONS (COMPLETE NECROPSY PERFORMED): *ALL TISSUES HERE EXAMINED AND HERE WITHIN NORMAL LIMITS* I END OF REPORT FOR ANIMAL 90A3795 I DURATION: ROUTE: SPECIES: 19-WEEK INHALATION RAT 1,1,1-T subchronic neurotoxicity APPENDIX Q; GROSS PATHOLOGY, GROUP A The Dow Chemical Company Study ID: K-001716-091 Volume 3 of 3, Page: 5 ANIMAL NUMBER: 90A3796 SEX: MALE DOSE: 0 PPM TEST MATERIAL: MODE OF DEATH: NECROPSY DATE: 1,1,1-T SACRIFICED 09/06/90 (DAY 101) FINAL BODY HEIGHT (GRAMS): 266.6 ORGAN HEIGHTS (GRAMS): BRAIN 1.601 GROSS PATHOLOGIC OBSERVATIONS (COMPLETE NECROPSY PERFORMED): ALL TISSUES HERE EXAMINED AND HERE HITHIN NORMAL LIMITS* t END OF REPORT FOR ANIMAL 90A3796 I DURATION: ROUTE: SPECIES: 19-WEEK INHALATION RAT SL 031051 ANIMAL NUMBER: 90A3797 SEX: MALE DOSE: 0 PPM TEST MATERIAL: MODE OF DEATH: NECROPSY DATE: 1, 1,1-T SACRIFICED 10/09/90 (DAY 134) FINAL BODY HEIGHT (GRAMS): 321.2 GROSS PATHOLOGIC OBSERVATIONS (COMPLETE NECROPSY PERFORMED): ALL TISSUES HERE EXAMINED AND HERE HITHIN NORMAL LIMITS* t END OF REPORT FOR ANIMAL 90A3797 I DURATION: ROUTE: SPECIES: 1 9-WEEK INHALATION RAT SL 031052 1,1,1-T subchronic neurotoxicity APPENDIX Q: GROSS PATHOLOGY, GROUP A The Dow Chemical Company Study ID: K-001716-091 Volume 3 of 3, Page: 6 ANIMAL NUMBER: 90A3798 SEX; MALE DOSE: 0 PPM TEST MATERIAL: MODE OF DEATH: NECROPSY DATE: 1,1,1-T SACRIFICED 09/06/90 (DAY 101) PINAL BODY HEIGHT (GRAMS): 307.4 ORGAN HEIGHTS (GRAMS): BRAIN 1.917 GROSS PATHOLOGIC OBSERVATIONS (COMPLETE NECROPSY PERFORMED): ALL TISSUES HERE EXAMINED AND WERE WITHIN NORMAL LIMITS* I END OF REPORT FOR ANIMAL 90A3798 I DURATION: ROUTE: SPECIES: 19-WEEK INHALATION RAT ANIMAL NUMBER: 90A3B06 SEX: MALE DOSE: 200 PPM TEST MATERIAL: MODE OF DEATH: NECROPSY DATE: 1,1,1-T SACRIFICED 10/09/90 (DAY 134) FINAL BODY WEIGHT (GRAMS); 383.9 GROSS PATHOLOGIC OBSERVATIONS (COMPLETE NECROPSY PERFORMED); ALL TISSUES WERE EXAMINED AND HERE WITHIN NORMAL LIMITS* I END OF REPORT FOR ANIMAL 90A3806 I DURATION: ROUTE: SPECIES: 1 9-WEEK INHALATION RAT i 1,1,1-T subchronic neurotoxicity APPENDIX Q: GROSS PATHOLOGY, GROUP A The Dow Chemical Company Study ID: K-0017I6-091 Volume 3 of 3, Page: 7 ANIMAL NUMBER: 90A3807 SEX: MALE DOSE: 200 PINAL BOD* WEIGHT (GRAMSI: 308.9 PPM TEST MATERIAL: MODE OF DEATH: . NECROPSY DATE: 1, 1, 1-T SACRIFICED 09/06/90 (DAY 101) ORGAN WEIGHTS (GRAMS): 8RAIN 1.787 GROSS PATHOLOGIC OBSERVATIONS (COMPLETE NECROPSY PERFORMED): "ALL TISSUES WERE EXAMINED AND WERE WITHIN NORMAL LIMITS* I END OP REPORT FOR ANIMAL 90A3807 t DURATION: ROUTE: SPECIES: 19-WEEK INHALATION RAT SL 031053 ANIMAL NUMBER: 90A3808 SEX: MALE DOSE: 200 FINAL BODY WEIGHT (GRAMS): 347.4 PPM TEST MATERIAL: MODE OF DEATH: NECROPSY DATE: 1, 1, 1-T SACRIFICED 10/09/90 (DAY 134) GROSS PATHOLOGIC OBSERVATIONS (COMPLETE NECROPSY PERFORMED); ALL TISSUES WERE EXAMINED AND WERE WITHIN NORMAL LIMITS* I END OF REPORT FOR ANIMAL 90A3808 I DURATION: ROUTE: SPECIES: 19-WEEK INHALATION RAT 1,1,1-T subchronic neurotoxicity APPENDIX Q: GROSS PATHOLOGY, GROUP A The Dow Chemical Company Study ID: K-001716-091 Volume 3 of 3, Page: 8 ANIMAL NUMBER: 90A3B09 SEX: MALE DOSE: 2 00 PINAL BODY WEIGHT (GRAMS): 317.5 PPM TEST MATERIAL: MODE OF DEATH: NECROPSY DATE: 1,1,1-T SACRIFICED 10/09/90 (DAY 134) GROSS PATHOLOGIC OBSERVATIONS (COMPLETE NECROPSY PERFORMED): *ALL TISSUES WERE EXAMINED AND HERE WITHIN NORMAL LIMITS* I END OF REPORT FOR ANIMAL 90A3809 I DURATION: ROUTE: SPECIES: 19-WEEK INHALATION RAT SL 031054 ANIMAL NUMBER: 90A3810 SEX: MALE DOSE: 200 PPM TEST MATERIAL: MODE OF DEATH: NECROPSY DATE: 1,1,1-T SPONTANEOUS 10/01/90 (DAY 126) DURATION: 19-WEEK ROUTE: INHALATION SPECIES: RAT GROSS PATHOLOGIC OBSERVATIONS (COMPLETE NECROPSY PERFORMED): GENERAL CONGESTION, VISCERA GENERAL COMMENT THE CAUSE OF DEATH WAS UNDETERMINED. THE CARCASS HAS AUTOLYZED. ALL OTHER TISSUES WERE WITHIN NORMAL LIMITS I END OF REPORT FOR ANIMAL 90A3810 I 1,1,1-T subchronic neurotoxicity APPENDIX Q: GROSS PATHOLOGY, GROUP A The Dow Chemical Company Study ID: K-00171fr091 Volume 3 ol 3, Page: 9 ANIMAL NUMBER: 90A3BI1 SEX: MALE DOSE: 200 FINAL BODY WEIGHT (GRAMS): 341.0 PPM TEST MATERIAL: MODE OF DEATH: NECROPSY DATE: 1,1,1-T SACRIFICED 09/06/90 (DAY 101) DURATION: ROUTE: SPECIES: 19-WEEK INHALATION RAT U) ORGAN WEIGHTS (GRAMS): f BRAIN I.854 031055 GROSS PATHOLOGIC OBSERVATIONS (COMPLETE NECROPSY PERFORMED): `ALL TISSUES WERE EXAMINED AND HERE WITHIN NORMAL LIMITS* I END OF REPORT FOR ANIMAL 90A381I I ANIMAL NUMBER: 90A3812 SEX: MALE DOSE: 200 FINAL BODY HEIGHT (GRAMS): 354.9 PPM TEST MATERIAL: MODE OF DEATH: NECROPSY DATE: 1,1,1-T SACRIFICED 10/09/90 (DAY 134) GROSS PATHOLOGIC OBSERVATIONS (COMPLETE NECROPSY PERFORMED): EXTERNAL AND SKIN ABSCESS, HEAD, FOCAL COMMENT: THERE IS PURULENT EXUDATE UNDERLYING AND SURROUNDING THE BASE OF THE ACRYLIC CAP. ALL OTHER TISSUES WERE WITHIN NORMAL LIMITS I END OF REPORT FOR ANIMAL 90A3812 I DURATION: ROUTE; SPECIES: 19-WEEK INHALATION RAT 1,1,1 -T subchronic neurotoxicity APPENDIX Q: GROSS PATHOLOGY, GROUP A The Dow Chemical Company Study ID: K-001716-091 Volume 3 of 3, Page: 10 ANIMAL NUMBER: 90A3820 SEX: MALE DOSE: 630 FINAL BODY WEIGHT (GRAMS): 333.5 PPM TEST MATERIAL: MODE OF DEATH: NECROPSY DATE: 1,1,1-T SACRIFICED 10/09/90 (DAY 134) DURATION: ROUTE: SPECIES: 19-WEEK INHALATION RAT SL 031056 GROSS PATHOLOGIC OBSERVATIONS (COMPLETE NECROPSY PERFORMED): 'ALL TISSUES WERE EXAMINED AND HERE WITHIN NORMAL LIMITS' I END OF REPORT FOR ANIMAL 90A3820 t ANIMAL NUMBER: 90A3821 SEX: MALE DOSE: 630 FINAL BODY HEIGHT (GRAMS): 290.6 PPM TEST MATERIAL: MODE OF DEATH: NECROPSY DATE: 1,1,1-T SACRIFICED 09/06/90 (DAY 101) ORGAN HEIGHTS (GRAMS): BRAIN 1.715 GROSS PATHOLOGIC OBSERVATIONS (COMPLETE NECROPSY PERFORMED): 'ALL TISSUES HERE EXAMINED AND WERE WITHIN NORMAL LIMITS* I END OF REPORT FOR ANIMAL 90A3821 t DURATION: ROUTE: SPECIES: 19-WEEK INHALATION RAT 1,1,1 -T subchronic neurotoxicity APPENDIX Q: GROSS PATHOLOGY, GROUP A The Dow Chemical Company Study ID: K-001716-091 Volume 3 ot 3, Page; 11 ANIMAL NUMBER: 90A3622 SEX: MALE DOSE: 630 PPM TEST MATERIAL: MODE OF DEATH: NECROPSY DATE: 1,1,1-T MORIBUND 10/01/90 (DAY 126) DURATION: ROUTE: SPECIES: 19-WEEK INHALATION RAT SL 03X057 GROSS PATHOLOGIC OBSERVATIONS (COMPLETE NECROPSY PERFORMED); DIGESTIVE TRACT CONTENTS - DARK GAS GENERAL FAT - DECREASED AMOUNT ORAL TISSUES ULCER, GINGIVA FRACTURED/MISSING TEETH, UPPER INCISORS, BILATERAL STOMACH EROSION(S) AND/OR ULCER(S), GLANDULAR MUCOSA, MULTIFOCAL GENERAL COMMENT THE MORIBUND CONDITION IS RELATED TO THE LOSS OF THE UPPER INCISORS, AND THE GINGIVAL ULCERATION. ALL OTHER TISSUES HERE HITHIN NORMAL LIMITS I END OF REPORT FOR ANIMAL 90A3822 t 1,1,1-T subchronic neurotoxicity APPENDIX Q: GROSS PATHOLOGY, GROUP A The Dow Chemical Company Study ID; K-001716-091 Volume 3 of 3, Page: 12 ANIMAL NUMBER: 90A3823 SEX: MALE DOSE: 30 FINAL BODY HEIGHT (GRAMS): 348.9 PPM TEST MATERIAL: MODE OF DEATH: NECROPSY DATE: 1,1,1-T SACRIFICED 10/09/90 (DAY 134) DURATION: ROUTE: SPECIES: 19-WEEK INHALATION RAT SL 031058 GROSS PATHOLOGIC OBSERVATIONS (COMPLETE NECROPSY PERFORMED): *ALL TISSUES HERE EXAMINED AND HERE WITHIN NORMAL LIMITS* I END OF REPORT FOR ANIMAL 90A3823 f ANIMAL NUMBER: 90A3824 SEX: MALE DOSE: 630 FINAL BODY HEIGHT (GRAMS): 337.6 PPM TEST MATERIAL: MODE OF DEATH: NECROPSY DATE: 1.1.1-T SACRIFICED 10/09/90 (DAY 134) DURATION: ROUTE: SPECIES; 19-HEEK INHALATION RAT GROSS PATHOLOGIC OBSERVATIONS (COMPLETE NECROPSY PERFORMED): `ALL TISSUES WERE EXAMINED AND HERE WITHIN NORMAL LIMITS* I END OF REPORT FOR ANIMAL 90A3624 I 1,1,1-T subchronic neurotoxicity APPENDIX Q: GROSS PATHOLOGY, GROUP A The Dow Chemical Company Study ID: K-001716-091 Volume 3 of 3, Page: 13 SL 031059 ANIMAL NUMBER: 90A3825 SEX: MALE DOSE: 30 PPM TEST MATERIAL: MODE OF DEATH: NECROPSY DATE: 1,1,1-T SACRIFICED 09/06/90 (DAY 101) FINAL BODY HEIGHT (GRAMS): 300.8 ORGAN HEIGHTS (GRAMS): BRAIN 1.805 GROSS PATHOLOGIC OBSERVATIONS (COMPLETE NECROPSY PERFORMED): EXTERNAL AND SKIN ABSCESS, HEAD, FOCAL COMMENT: THERE IS PURULENT EXUDATE UNDERLYING AND SURROUNDING THE BASE OF ONE ELECTRODE LOCATED ON THE ANTERIOR POLE OF THE ACRYLIC CAP. THE EXUDATE IS BETHEEN THE DERMIS AND CALVARIAL BONE. ALL OTHER TISSUES HERE WITHIN NORMAL LIMITS t END OF REPORT FOR ANIMAL 90A3825 t DURATION; ROUTE: SPECIES: 19-HEEK INHALATION RAT 1,1,1-T subchronic neurotoxicity APPENDIX Q: GROSS PATHOLOGY, GROUP A The Dow Chemical Company Study ID: K-001716-091 Volume 3 of 3, Page: 14 ANIMAL NUMBER: 90A3826 SEX: MALE DOSE: 630 PINAL BODY HEIGHT (GRAMS); 304.3 PPM TEST MATERIAL: MODE OF DEATH: NECROPSY DATE: 1,1.1-T SACRIFICED 09/06/90 (DAY 101) ORGAN HEIGHTS (GRAMS): BRAIN 1.858 GROSS PATHOLOGIC OBSERVATIONS (COMPLETE NECROPSY PERFORMED): `ALL TISSUES HERE EXAMINED AND HERE WITHIN NORMAL LIMITS* th I END OF REPORT FOR ANIMAL 90A3826 t DURATION: ROUTE: SPECIES: 19-WEEK INHALATION RAT 03106 ANIMAL NUMBER: 90A3834 SEX: MALE DOSE: 2000 FINAL BODY HEIGHT (GRAMS): 341.6 PPM TEST MATERIAL: MODE OF DEATH: NECROPSY DATE: 1,1,1-T SACRIFICED 10/09/90 (DAY 134) DURATION: ROUTE: SPECIES: 19-WEEK INHALATION RAT GROSS PATHOLOGIC OBSERVATIONS (COMPLETE NECROPSY PERFORMED): `ALL TISSUES HERE EXAMINED AND HERE HITHIN NORMAL LIMITS* I END OF REPORT FOR ANIMAL 90A3834 I 1,1,1-T subchronic neurotoxicity APPENDIX Q: GROSS PATHOLOGY, GROUP A The Dow Chemical Company Study ID: K-001716-091 Volume 3 of 3, Page: 15 ANIMAL NUMBER: 90A383S SEX: MALE DOSE: 2 000 FINAL BODY WEIGHT (GRAMS): 346.9 PPM TEST MATERIAL: MODE OF DEATH: NECROPSY DATE: 1,1,1-T SACRIFICED 10/09/90 (DAY 134) DURATION: ROUTE: SPECIES: 19-WEEK INHALATION RAT GROSS PATHOLOGIC OBSERVATIONS (COMPLETE NECROPSY PERFORMED): *ALL TISSUES HERE EXAMINED AND HERE WITHIN NORMAL LIMITS* t END OF REPORT FOR ANIMAL 90A3835 I SL 031061 ANIMAL NUMBER: 90A3836 SEX: MALE DOSE: 2000 PPM TEST MATERIAL: MODE OF DEATH: NECROPSY DATE: 1,1,1-T SACRIFICED 08/29/90 (DAY 93) DURATION: ROUTE: SPECIES: 19-WEEK INHALATION RAT GROSS PATHOLOGIC OBSERVATIONS (COMPLETE NECROPSY PERFORMED): EXTERNAL AND SKIN HEMORRHAGE, HEAD COMMENT: THE HEMORRHAGE RESULTED FROM LOSS OF THE EPIDURAL ELECTRODES. GENERAL COMMENT THE ANIMAL HAS SACRIFICED PRIOR TO STUDY TERMINATION DUE TO LOSS OF THE EPIDURAL ELECTRODES. ALL OTHER TISSUES HERE HITHIN NORMAL LIMITS I END OF REPORT FOR ANIMAL 90A3836 t 1,1,1-T subchronic neurotoxicity APPENDIX Q: GROSS PATHOLOGY, GROUP A The Dow Chemical Company Study ID: K-001716-091 Volume 3 of 3, Page: 16 ANIMAL NUMBER: 90A3837 SEX: MALE DOSE: 2000 PINAL BODY WEIGHT (GRAMS!: 345.1 PPM TEST MATERIAL: MODE OF DEATH: NECROPSY DATE: 1, 1- 1-T SACRIFICED 09/06/90 (DAY 101) ORGAN WEIGHTS (GRAMS); BRAIN 1.873 GROSS PATHOLOGIC OBSERVATIONS (COMPLETE NECROPSY PERFORMED): ALL TISSUES WERE EXAMINED AND WERE WITHIN NORMAL LIMITS* I END OF REPORT FOR ANIMAL 90A3837 I DURATION: ROUTE: SPECIES: 19-WEEK INHALATION RAT SL 031062 ANIMAL NUMBER: 90A383B SEX: MALE DOSE: 2000 FINAL BODY WEIGHT (GRAMS): 290.4 PPM TEST MATERIAL: MODE OF DEATH: NECROPSY DATE: 1.1,1-T SACRIFICED 09/06/90 (DAY 101) ORGAN WEIGHTS (GRAMS): BRAIN 1.925 GROSS PATHOLOGIC OBSERVATIONS (COMPLETE NECROPSY PERFORMED): ALL TISSUES WERE EXAMINED AND WERE WITHIN NORMAL LIMITS* I END OF REPORT FOR ANIMAL 90A3638 t DURATION: ROUTE: SPECIES: 19-WEEK INHALATION RAT 1,1,1 -T subchronic neurotoxicity APPENDIX Q: GROSS PATHOLOGY, GROUP A The Dow Chemical Company Study ID: K-001716-091 Volume 3 of 3, Page: 17 031063 ANIMAL NUMBER: 90A3839 SEX; MALE DOSE; 2000 PPM CO TEST MATERIAL: MODE OF DEATH: NECROPSY DATE: 1,1,1-T SACRIFICED 09/27/90 (DAY 122) DURATION: ROUTE: SPECIES: 19-WEEK INHALATION RAT GROSS PATHOLOGIC OBSERVATIONS (COMPLETE NECROPSY PERFORMED); `ALL TISSUES HERE EXAMINED AND HERE HITI1IN NORMAL LIMITS* GENERAL COMMENT THE ANIMAL HAS SACRIFICED PRIOR TO STUDY TERMINATION DUE TO THE LOSS OF THE EPIDURAL ELECTRODE. I END OF REPORT FOR ANIMAL 90A3839 I ANIMAL NUMBER: 90A3840 SEX: MALE DOSE: 2000 FINAL BODY HEIGHT (GRAMS): 269.3 PPM TEST MATERIAL: MODE OF DEATH: NECROPSY DATE: 1,1,1-T SACRIFICED 10/09/90 (DAY 134) DURATION: ROUTE: SPECIES: 1 9-WEEK INHALATION RAT GROSS PATHOLOGIC OBSERVATIONS (COMPLETE NECROPSY PERFORMED): `ALL TISSUES HERE EXAMINED AND HERE WITHIN NORMAL LIMITS* I END OF REPORT FOR ANIMAL 90A3840 I 1,1,1-T subchronic neurotoxicity APPENDIX Q: GROSS PATHOLOGY, GROUP A The Dow Chemical Company Study ID: K-001716-091 Volume 3 of 3, Page: 18 ANIMAL NUMBER: 90A3848 SEX: FEMALE DOSE: 0 PPM TEST MATERIAL: MODE OF DEATH: NECROPSY DATE: 1,1,1-T SACRIFICED 10/09/90 (DAY 134) FINAL BODY WEIGHT (GRAMS): 175.0 GROSS PATHOLOGIC OBSERVATIONS (COMPLETE NECROPSY PERFORMED): `ALL TISSUES WERE EXAMINED AND WERE WITHIN NORMAL LIMITS* t END OF REPORT FOR ANIMAL 90A3848 I DURATION: ROUTE: SPECIES: 19-WEEK INHALATION RAT SL 031064 ANIMAL NUMBER: 90A3849 SEX: FEMALE DOSE: 0 PPM TEST MATERIAL: MODE OF DEATH: NECROPSY DATE: 1,1,1-T SACRIFICED 10/09/90 (DAY 134) FINAL BODY WEIGHT (GRAMS): 185.8 GROSS PATHOLOGIC OBSERVATIONS (COMPLETE NECROPSY PERFORMED): *ALL TISSUES WERE EXAMINED AND WERE WITHIN NORMAL LIMITS* I END OF REPORT FOR ANIMAL 90A3849 I DURATION: ROUTE: SPECIES; 19-WEEK INHALATION RAT ANIMAL NUMBER: 90A3850 SEX: FEMALE DOSE: 0 PPM TEST MATERIAL: MODE OF DEATH: NECROPSY DATE: 1.1,1-T SACRIFICED 10/09/90 (DAY 134) FINAL BODY WEIGHT (GRAMS): 185.3 GROSS PATHOLOGIC OBSERVATIONS (COMPLETE NECROPSY PERFORMED): ALL TISSUES WERE EXAMINED AND HERE WITHIN NORMAL LIMITS* I END OF REPORT FOR ANIMAL 90A3850 I DURATION: ROUTE: SPECIES: 19-WEEK INHALATION RAT 1,1,1-T subchronic neurotoxicity APPENDIX Q: GROSS PATHOLOGY, GROUP A The Dow Chemical Company Study ID: K-0Q1716-O91 Volume 3 of 3, Page: 19 ANIMAL NUMBER: 90A3851 SEX: FEMALE DOSE: 0 PPM FINAL BODY HEIGHT (GRAMS): 175.3 TEST MATERIAL: MODE OF DEATH: NECROPSY DATE: 1,1,1-T SACRIFICED 10/09/90 (DAY 134) DURATION: ROUTE: SPECIES: 19-WEEK INHALATION RAT SL 031065 GROSS PATHOLOGIC OBSERVATIONS (COMPLETE NECROPSY PERFORMED): `ALL TISSUES HERE EXAMINED AND HERE HITHIN NORMAL LIMITS* I END OF REPORT FOR ANIMAL 90A3851 I ANIMAL NUMBER: 90A3852 SEX: FEMALE DOSE: 0 PPM FINAL BODY WEIGHT (GRAMS): 174.9 TEST MATERIAL: MODE OF DEATH: NECROPSY DATE: 1. 1, 1-T SACRIFICED 09/06/90 (DAY 101} ORGAN WEIGHTS (GRAMS): BRAIN 1.610 GROSS PATHOLOGIC OBSERVATIONS (COMPLETE NECROPSY PERFORMED): ALL TISSUES HERE EXAMINED AND HERE WITHIN NORMAL LIMITS* I END OF REPORT FOR ANIMAL 90A3852 I DURATION: ROUTE: SPECIES: 1 9-WEEK INHALATION RAT 1,1,1-T subchronic neurotoxicity APPENDIX Q: GROSS PATHOLOGY, GROUP A The Dow Chemical Company Study ID: K-001716-091 Volume 3 of 3, Page: 20 ANIMAL NUMBER: 90A3853 SEX: FEMALE DOSE: 0 PPM FINAL BODY WEIGHT (GRAMS): 181.1 TEST MATERIAL: MODE OF DEATH: NECROPSY DATE: 1,1,I-T SACRIFICED 10/09/90 (DAY 134> DURATION: ROUTE: SPECIES: 19-WEEK INHALATION RAT GROSS PATHOLOGIC OBSERVATIONS (COMPLETE NECROPSY PERFORMED): `ALL TISSUES HERE EXAMINED AND WERE WITHIN NORMAL LIMITS* I END OF REPORT FOR ANIMAL 90A3853 t SL 031066 ANIMAL NUMBER: 90A3854 SEX: FEMALE DOSE: O PPM FINAL BODY WEIGHT (GRAMS): 174.6 TEST MATERIAL: MODE OF DEATH: NECROPSY DATE: 1,1,1-T SACRIFICED 09/06/90 (DAY 101) ORGAN WEIGHTS (GRAMS): BRAIN 1.719 GROSS PATHOLOGIC OBSERVATIONS (COMPLETE NECROPSY PERFORMED): `ALL TISSUES HERE EXAMINED AND HERE WITHIN NORMAL LIMITS* I END OF REPORT FOR ANIMAL 90A38S4 I DURATION: ROUTE: SPECIES: 19-WEEK INHALATION RAT 1,1,1-T subchronic neurotoxicity APPENDIX Q: GROSS PATHOLOGY, GROUP A The Dow Chemical Company Study ID: K-001716-091 Volume 3 ot 3, Page: 21 ANIMAL NUMBER: 90A3662 SEX: FEMALE DOSE: 200 PPM FINAL BODY HEIGHT (GRAMS): 172.1 TEST MATERIAL: MODE OF DEATH: NECROPSY DATE: 1, 1, 1-T SACRIFICED 09/06/90 (DAY 101) DURATION: ROUTE: SPECIES: 1 9-WEEK INHALATION RAT ORGAN HEIGHTS (GRAMS): CO BRAIN t-l 1.714 GROSS PATHOLOGIC OBSERVATIONS (COMPLETE NECROPSY PERFORMED): `ALL TISSUES HERE EXAMINED AND WERE WITHIN NORMAL LIMITS* I END OF REPORT FOR ANIMAL 90A3862 I ANIMAL NUMBER: 90A3863 SEX: FEMALE DOSE: 200 PPM FINAL BODY HEIGHT (GRAMS): 160.1 TEST MATERIAL: MODE OF DEATH: NECROPSY DATE: 1, 1, 1-T SACRIFICED 10/09/90 (DAY 134) DURATION: ROUTE: SPECIES: 19-WEEK INHALATION RAT GROSS PATHOLOGIC OBSERVATIONS (COMPLETE NECROPSY PERFORMED): ALL TISSUES HERE EXAMINED AND HERE HITHIN NORMAL LIMITS* I END OF REPORT FOR ANIMAL 90A3863 I * g0T0 I 1,1,1-T subchronic neurotoxicity APPENDIX Q: GROSS PATHOLOGY, GROUP A The Dow Chemical Company Study ID: K-001716-091 Volume 3 of 3, Page: 22 ANIMAL NUMBER: 90A3864 SEX: FEMALE DOSE: 200 PPM FINAL BOD* HEIGHT (GRAMS): 166.6 TEST MATERIAL: MODE OF DEATH: NECROPSY DATE: 1, 1, 1-T SACRIFICED 09/06/90 (DAY 101) DURATION: ROUTE: SPECIES: 19-WEEK INHALATION RAT ORGAN HEIGHTS (GRAMS): U) r1 BRAIN 1.658 031068 GROSS PATHOLOGIC OBSERVATIONS (COMPLETE NECROPSY PERFORMED): ALL TISSUES WERE EXAMINED AND WERE WITHIN NORMAL LIMITS* I END OF REPORT FOR ANIMAL 90A3864 t ANIMAL NUMBER: 90A3865 SEX: FEMALE DOSE: 200 PPM FINAL BOD* HEIGHT (GRAMS): 163.4 TEST MATERIAL: MODE OF DEATH: NECROPSY DATE: 1,1,1-T SACRIFICED 09/06/90 (DA* 101) ORGAN WEIGHTS (GRAMS): BRAIN 1.664 GROSS PATHOLOGIC OBSERVATIONS (COMPLETE NECROPSY PERFORMED): ALL TISSUES WERE EXAMINED AND HERE WITHIN NORMAL LIMITS* I END OF REPORT FOR ANIMAL 90A3865 I DURATION: ROUTE: SPECIES; 19-WEEK INHALATION RAT 1,1,1-T subchronic neurotoxicity APPENDIX Q: GROSS PATHOLOGY, GROUP A The Dow Chemical Company Study ID: K-001716-091 Volume 3 of 3, Page: 23 ANIMAL NUMBER: 90A3866 SEX: FEMALE DOSE: 200 PPM TEST MATERIAL: MODE OF DEATH: NECROPSY DATE: 1,1,1-T SPONTANEOUS 08/02/90 (DAY 66) DURATION: ROUTE: SPECIES: 19-WEEK INHALATION RAT SL 031069 GROSS PATHOLOGIC OBSERVATIONS (COMPLETE NECROPSY PERFORMED): GENERAL CONGESTION, VISCERA GENERAL COMMENT THE GROSS OBSERVATION OF GENERAL VISCERAL CONGESTION IS CONSISTENT WITH THE CLINICAL DIAGNOSIS OF ANESTHETIC DEATH. ALL OTHER TISSUES MERE WITHIN NORMAL LIMITS I END OF REPORT FOR ANIMAL 90A3B66 I ANIMAL NUMBER: 90A3867 SEX: FEMALE DOSE: 200 PPM FINAL BODY HEIGHT (GRAMS): 1)8.4 TEST MATERIAL: MODE OF DEATH: NECROPSY DATE: 1,1, 1-T SACRIFICED 10/09/90 (DAY 134) DURATION: ROUTE: SPECIES: 19-NEEK INHALATION RAT GROSS PATHOLOGIC OBSERVATIONS (COMPLETE NECROPSY PERFORMED): `ALL TISSUES HERE EXAMINED AND HERE WITHIN NORMAL LIMITS* I END OF REPORT FOR ANIMAL 90A3867 t 1,1,1-T subchronic neurotoxicity APPENDIX Q: GROSS PATHOLOGY, GROUP A The Dow Chemical Company Study ID: K-001716-091 Volume 3 of 3, Page: 24 ANIMAL NUMBER: 90A3868 SEX: FEMALE DOSE: 200 PPM PINAL BODY HEIGHT (GRAMS): 161.9 TEST MATERIAL: MODE OF DEATH: NECROPSY DATE: 1,1,1-T SACRIFICED 10/09/90 (DAY 134) DURATION: ROUTE: SPECIES: 19-HEEK INHALATION RAT SL 031070 GROSS PATHOLOGIC OBSERVATIONS (COMPLETE NECROPSY PERFORMED): ALL TISSUES HERE EXAMINED AND HERE WITHIN NORMAL LIMITS* t END OF REPORT FOR ANIMAL 90A386B t ANIMAL NUMBER: 90A387G SEX: FEMALE DOSE: 630 PPM FINAL BODY HEIGHT (GRAMS): 187.8 TEST MATERIAL: MODE OF DEATH: NECROPSY DATE: 1,1, 1-T SACRIFICED 10/09/90 (DAY 134) DURATION: ROUTE: SPECIES: 19-HEEK INHALATION RAT GROSS PATHOLOGIC OBSERVATIONS (COMPLETE NECROPSY PERFORMED): ALL TISSUES HERE EXAMINED AND HERE WITHIN NORMAL LIMITS* I END OF REPORT FOR ANIMAL 90A3876 I 1,1,1-T subchronic neurotoxicity APPENDIX Q: GROSS PATHOLOGY, GROUP A The Dow Chemical Company Study ID: K-001716-091 Volume 3 of 3, Page: 25 ANIMAL NUMBER: 90A3877 SEX: FEMALE DOSE: 630 PPM TEST MATERIAL: MODE OF DEATH: NECROPSY DATE: 1,1,1-T SACRIFICED 09/06/90 (DAY 101) FINAL BODY HEIGHT (GRAMS); 184.6 ORGAN HEIGHTS (GRAMS); BRAIN , 1.655 0 GROSS PATHOLOGIC OBSERVATIONS (COMPLETE NECROPSY PERFORMED): EXTERNAL AND SKIN ABSCESS, HEAD, FOCAL COMMENT; THERE IS PURULENT EXUDATE AROUND THE BASE OF THE MOST CAUDAL ELECTRODE. THE EXUDATE IS LOCATED BETWEEN THE DERMIS AND THE CALVARIAL BONE. ALL OTHER TISSUES HERE WITHIN NORMAL LIMITS I END OF REPORT FOR ANIMAL 90A3B77 I DURATION: ROUTE: SPECIES: 1 9-WEEK INHALATION RAT T^ 0 ICO ANIMAL NUMBER: 90A3878 SEX: FEMALE DOSE: 630 PPM FINAL BODY HEIGHT (GRAMS): 172.6 TEST MATERIAL: MODE OF DEATH: NECROPSY DATE: 1,1,1-T SACRIFICED 10/09/90 (DAY 134) DURATION: ROUTE: SPECIES: 19-WEEK INHALATION RAT GROSS PATHOLOGIC OBSERVATIONS (COMPLETE NECROPSY PERFORMED): *ALL TISSUES HERE EXAMINED AND HERE WITHIN NORMAL LIMITS* I END OF REPORT FOR ANIMAL 90A3878 I 1,1,1-T subchronic neurotoxicity APPENDIX Q: GROSS PATHOLOGY, GROUP A The Dow Chemical Company Study ID: K-001716-091 Volume 3 of 3, Page: 26 ANIMAL NUMBER: 90A3879 SEX: FEMALE DOSE: 630 PPM FINAL BOOK WEIGHT (GRAMS): I 90.B TEST MATERIAL: MODE OF DEATH: NECROPSY DATE: 1,1,1-T SACRIFICED 10/09/90 (DAY 134) DURATION: ROUTE: SPECIES: 19-HEEK INHALATION RAT GROSS PATHOLOGIC OBSERVATIONS (COMPLETE NECROPSY PERFORMED): ALL TISSUES WERE EXAMINED AND WERE WITHIN NORMAL LIMITS* t END OF REPORT FOR ANIMAL 90A3B79 I SL 03107 ANIMAL NUMBER: 90A3BB0 SEX: FEMALE DOSE: 630 PPM to TEST MATERIAL: MODE OF DEATH: NECROPSY DATE: 1, 1,1-T SACRIFICED 09/18/90 (DAY 113) GROSS PATHOLOGIC OBSERVATIONS (COMPLETE NECROPSY PERFORMED): ALL TISSUES WERE EXAMINED AND HERE WITHIN NORMAL LIMITS* GENERAL COMMENT THE ANIMAL HAS SACRIFICED PRIOR TO THE TERMINATION OF THE RECOVERY PERIOD DUE TO LOSS OF THE ACRYLIC CAP. I END OF REPORT FOR ANIMAL 90A38B0 I DURATION: ROUTE: SPECIES: 19-WEEK INHALATION RAT 1,1,1 -T subchronic neurotoxicity APPENDIX Q: GROSS PATHOLOGY, GROUP A The Dow Chemical Company Study ID: K-001716-091 Volume 3 of 3, Page: 27 ANIMAL NUMBER: 90A388I SEX: FEMALE DOSE: 630 PPM PINAL BODY WEIGHT (GRAMS!: 171.8 TEST MATERIAL: MODE OF DEATH: NECROPSY DATE: 1,1, 1-T SACRIFICED 10/09/90 (DAY 134) DURATION: ROUTE: SPECIES: 19-WEEK INHALATION RAT GROSS PATHOLOGIC OBSERVATIONS (COMPLETE NECROPSY PERFORMED): `ALL TISSUES WERE EXAMINED AND WERE WITHIN NORMAL LIMITS* t END OP REPORT FOR ANIMAL 90A3881 I ANIMAL NUMBER: 90A38B2 SEX: FEMALE DOSE: 630 PPM PINAL BODY WEIGHT (GRAMS): 180.5 TEST MATERIAL: MODE OF DEATH: NECROPSY DATE: 1,1,1-T SACRIFICED 09/06/90 (DAY 101) DURATION: ROUTE: SPECIES: 19-WEEK INHALATION RAT ORGAN WEIGHTS (GRAMS): BRAIN U5 1.697 GROSS PATHOLOGIC OBSERVATIONS (COMPLETE NECROPSY PERFORMED): 031073 *ALL TISSUES HERE EXAMINED AND WERE WITHIN NORMAL LIMITS* t END OP REPORT FOR ANIMAL 90A3882 t I 1,1,1-T subchronic neurotoxicity APPENDIX Q: GROSS PATHOLOGY, GROUP A The Dow Chemical Company Study ID: K-001716-091 Volume 3 of 3, Page: 28 ANIMAL NUMBER: 90A3890 SEX: FEMALE DOSE: 2000 PPM FINAL BODY WEIGHT IGRAMS); 180.1 TEST MATERIAL: MODE OF DEATH: NECROPSY DATE: 1,1,1-T SACRIFICED 10/09/90 (DAY 134) DURATION: ROUTE: SPECIES: 19-WEEK INHALATION RAT GROSS PATHOLOGIC OBSERVATIONS (COMPLETE NECROPSY PERFORMED): *ALL TISSUES WERE EXAMINED AND WERE WITHIN NORMAL LIMITS* I END OF REPORT FOR ANIMAL 90A3890 I SL 031074 ANIMAL NUMBER: 90A3891 SEX: DOSE: FEMALE 2000 PPM TEST MATERIAL: MODE OF DEATH: NECROPSY DATE: 1,1, 1-T SACRIFICED 09/06/90 (DAY 101) FINAL BODY WEIGHT (GRAMS): 173.2 ORGAN WEIGHTS (GRAMS): BRAIN 1.680 GROSS PATHOLOGIC OBSERVATIONS (COMPLETE NECROPSY PERFORMED): ALL TISSUES HERE EXAMINED AND WERE WITHIN NORMAL LIMITS* I END OF REPORT FOR ANIMAL 90A3891 I DURATION: ROUTE: SPECIES: 19-WEEK INHALATION RAT 1,1,1-T subchronic neurotoxicity APPENDIX Q: GROSS PATHOLOGY, GROUP A The Dow Chemical Company Study ID: K-001716-091 Volume 3 of 3, Page: 29 ANIMAL NUMBER: 90A3892 SEX: FEMALE DOSE: 2000 PPM TEST MATERIAL: MODE OF DEATH: NECROPSY DATE: 1,1,1-T SACRIFICED 09/06/90 (DAY 101) FINAL BODY HEIGHT (GRAMS): 174.8 ORGAN HEIGHTS (GRAMS): BRAIN 1.697 GROSS PATHOLOGIC OBSERVATIONS (COMPLETE NECROPSY PERFORMED): ALL TISSUES HERE EXAMINED AND HERE HITHIN NORMAL LIMITS* t END OF REPORT FOR ANIMAL 90A3S92 I DURATION: ROUTE: SPECIES: 19-HEEK INHALATION RAT SL 031075 ANIMAL NUMBER: 90A3893 SEX: FEMALE DOSE: 2000 PPM TEST MATERIAL: MODE OF DEATH: NECROPSY DATE: 1,1,1-T SACRIFICED 09/06/90 (DAY 101) FINAL BODY HEIGHT (GRAMS): 177.3 ORGAN HEIGHTS (GRAMS): BRAIN 1.782 GROSS PATHOLOGIC OBSERVATIONS (COMPLETE NECROPSY PERFORMED): ALL TISSUES HERE EXAMINED AND HERE HITHIN NORMAL LIMITS* I END OF REPORT FOR ANIMAL 90A3893 I DURATION: ROUTE: SPECIES: 19-HEEK INHALATION RAT 1,1,1-T subchronic neurotoxicity APPENDIX Q: GROSS PATHOLOGY, GROUP A The Dow Chemical Company Study ID: K-001716-091 Volume 3 of 3, Page: 30 ANIMAL NUMBER: 90A3894 SEX: FEMALE DOSE: 2000 PPM TEST MATERIAL: MODE OF DEATH: NECROPSY DATE: 1,1,1-T SPONTANEOUS 08/02/90 (DAY 66) DURATION: ROUTE: SPECIES: 19-WEEK INHALATION RAT GROSS PATHOLOGIC OBSERVATIONS (COMPLETE NECROPSY PERFORMED): ALL TISSUES HERE EXAMINED AND HERE WITHIN NORMAL LIMITS* GENERAL COMMENT THE CAUSE OF DEATH HAS NOT DETERMINED. II END OF REPORT FOR ANIMAL 90A3894 031076 ANIMAL NUMBER: 90A3895 SEX: DOSE: FEMALE 2000 PPM FINAL BODY HEIGHT (GRAMS): 187.5 TEST MATERIAL: MODE OF DEATH: NECROPSY DATE: 1,1,1-T SACRIFICED 10/09/90 (DAY 134) DURATION: ROUTE: SPECIES: 19-WEEK INHALATION RAT GROSS PATHOLOGIC OBSERVATIONS (COMPLETE NECROPSY PERFORMED): ALL TISSUES HERE EXAMINED AND HERE WITHIN NORMAL LIMITS* I END OF REPORT FOR ANIMAL 90A3895 I ^O r, 8 o Co 1,1,1-T subchronic neurotoxicity APPENDIX Q: GROSS PATHOLOGY, GROUP A The Dow Chemical Company Study ID: K-001716-091 Volume 3 of 3, Page: 31 ANIMAL NUMBER: 90A3B96 SEX: FEMALE DOSE: 2000 PPM TEST MATERIAL: MODE OF DEATH: NECROPSY DATE: 1. 1,1-T SPONTANEOUS 00/03/90 (DAY 61) DURATION: ROUTE: SPECIES: 1 9-WEEK INHALATION RAT GROSS PATHOLOGIC OBSERVATIONS (COMPLETE NECROPSY PEREORMED): BONE FRACTURE - RECENT, VERTEBRAL - THORACIC HEMORRHAGE, CRANIAL CAVITY COMMENT: THE FRACTURE IS LOCATED IN T3 AND/OR T4. COMMENT: THERE IS A SMALL AMOUNT OF UNCLOTTED BLOOD IN THE BASE OF THE CALVARIUM. GENERAL CONGESTION, VISCERA LUNGS CONGESTION SPINAL CORO - THORACIC HEMORRHAGE THORACIC CAVITY HEMOTHORAX GENERAL COMMENT TRAUMA RESULTING IN VERTEBRAL BODY FRACTURE AND HEMOTHORAX (PROBABLY DUE TO BLOOD VESSEL RUPTURE) HAS THE CAUSE OF DEATH. ALL OTHER TISSUES HERE H1THIN NORMAL LIMITS I END OF REPORT FOR ANIMAL 90A3B96 I 1,1,1-T subchronic neurotoxicity APPENDIX Q: GROSS PATHOLOGY, GROUP B The Dow Chemical Company Study ID: K-001716-091 Volume 3 of 3, Page: 32 ANIMAL NUMBER: 90A3799 SEX: MALE DOSE: 0 FINAL BODY WEIGHT (GRAMS): 364.7 PPM TEST MATERIAL: MODE OF DEATH: NECROPSY DATE: 1, 1,1-T SACRIFICED 10/16/90 (DAY 134) DURATION: ROUTE: SPECIES: 13-HEEK INHALATION RAT GROSS PATHOLOGIC OBSERVATIONS (COMPLETE NECROPSY PERFORMED): `ALL TISSUES MERE EXAMINED AND HERE WITHIN NORMAL LIMITS* I END OF REPORT FOR ANIMAL 90A3799 I SL 031078 ANIMAL NUMBER: 90A3800 SEX: MALE DOSE: 0 FINAL BODY HEIGHT (GRAMS): 323.2 PPM TEST MATERIAL: MODE OF DEATH: NECROPSY DATE: 1,1,1-T SACRIFICED 10/16/90 (DAY 134) DURATION: ROUTE: SPECIES: 13-WEEK INHALATION RAT GROSS PATHOLOGIC OBSERVATIONS (COMPLETE NECROPSY PERFORMED): `ALL TISSUES HERE EXAMINED AND HERE WITHIN NORMAL LIMITS* I END OF REPORT FOR ANIMAL 90A3800 t 1,1/1 -T subchronic neurotoxicity APPENDIX Q: GROSS PATHOLOGY, GROUP B The Dow Chemical Company Study ID: K-001716-091 Volume 3 of 3, Page: 33 ANIMAL NUMBER: 90A3801 SEX: MALE DOSE: 0 PPM TEST MATERIAL: MODE OF DEATH: NECROPSY DATE: 1,1,1-T SACRIFICED 09/05/90 (DAY 93) DURATION: ROUTE: SPECIES: 13-WEEK INHALATION RAT GROSS PATHOLOGIC OBSERVATIONS (COMPLETE NECROPSY PERFORMED): *ALL TISSUES WERE EXAMINED AND WERE WITHIN NORMAL LIMITS* GENERAL COMMENT THE ANIMAL WAS SACRIFICED PRIOR TO STUDY TERMINATION DUE TO LOSS OF THE EPIDURAL ELECTRODES. t END OF REPORT FOR ANIMAL 90A3801 I SL 031079 ANIMAL NUMBER: 90A3802 SEX: MALE DOSE: 0 FINAL BODY WEIGHT (GRAMS): 306.2 PPM TEST MATERIAL: MODE OF DEATH: NECROPSY DATE: 1,1, 1-T SACRIFICED 10/16/90 (DAY 134) GROSS PATHOLOGIC OBSERVATIONS (COMPLETE NECROPSY PERFORMED): EXTERNAL AND SKIN ABSCESS, HEAD, FOCAL COMMENT: THERE IS PURULENT EXUDATE UNDERLYING AND SURROUNDING THE ACRYLIC CAP. THE EXUDATE IS BETWEEN THE DERMIS AND CALVARIAL BONE. ALL OTHER TISSUES WERE WITHIN NORMAL LIMITS I END OF REPOHT FOR ANIMAL 90A3802 I DURATION: ROUTE: SPECIES: 13-WEEK INHALATION RAT 1,1,1-T subchronic neurotoxicity APPENDIX Q: GROSS PATHOLOGY, GROUP B The Dow Chemical Company Study ID: K-001716-091 Volume 3 of 3, Page: 34 ANIMAL NUMBER: 90A3B03 SEX: MALE DOSE: 0 PINAL BODY HEIGHT (GRAMS): 343.5 PPM TEST MATERIAL: MODE OF DEATH: NECROPSY DATE: 1,1,1-T SACRIFICED 10/16/90 (DAY 134) CO GROSS PATHOLOGIC OBSERVATIONS (COMPLETE NECROPSY PERPORMED): `ALL TISSUES HERE EXAMINED AND HERE HITHIN NORMAL LIMITS* t tend of Report for animal 90A3803 DURATION: ROUTE; SPECIES: 13-HEEK INHALATION RAT 080l 0 ANIMAL NUMBER: 90A3B04 SEX: MALE DOSE: 0 PINAL BODY HEIGHT (GRAMS): 319.7 PPM TEST MATERIAL: MODE OF DEATH: NECROPSY DATE: 1,1,I-T SACRIFICED 09/13/90 (DAY 101) ORGAN HEIGHTS (GRAMS): BRAIN 1.937 GROSS PATHOLOGIC OBSERVATIONS (COMPLETE NECROPSY PERPORMED): `ALL TISSUES HERE EXAMINED AND HERE HITHIN NORMAL LIMITS* I END OF REPORT FOR ANIMAL 90A3804 I DURATION: ROUTE: SPECIES: 13-HEEK INHALATION RAT 1,1,1 -T subchronic neurotoxicity APPENDIX Q: GROSS PATHOLOGY, GROUP B The Dow Chemical Company Study ID: K-001716D91 Volume 3 of 3, Page: 35 ANIMAL NUMBER: 90A3805 SEX: MALE DOSE: 0 FINAL BODY WEIGHT (GRAMS): 294.2 PPM TEST MATERIAL: MODE OF DEATH: NECROPSY DATE: 1, 1, 1-T SACRIFICED 09/13/90 (DAY 101) ORGAN WEIGHTS (GRAMS): BRAIN 1,902 GROSS PATHOLOGIC OBSERVATIONS (COMPLETE NECROPSY PERFORMED): *ALL TISSUES HERE EXAMINED AND HERE WITHIN NORMAL LIMITS* I END OF REPORT FOR ANIMAL 90A3B05 I DURATION: ROUTE: SPECIES: 13-WEEK INHALATION RAT 031081 ANIMAL NUMBER: 90A3813 SEX: MALE DOSE: 200 FINAL BODY WEIGHT (GRAMS): 311.6 PPM TEST MATERIAL: MODE OF DEATH: NECROPSY DATE: 1,1,1-T SACRIFICED 09/13/90 (DAY 101) ORGAN WEIGHTS (GRAMS): BRAIN 1.862 GROSS PATHOLOGIC OBSERVATIONS (COMPLETE NECROPSY PERFORMED): *ALL TISSUES WERE EXAMINED AND HERE HITHIN NORMAL LIMITS* I END OF REPORT FOR ANIMAL 90A3813 I DURATION: ROUTE: SPECIES: 13-WEEK INHALATION RAT SL 031082 1,1,1-T subchronic neurotoxicity APPENDIX Q: GROSS PATHOLOGY, GROUP B The Dow Chemical Company Study ID: KTJ01716-091 Volume 3 of 3, Page: 36 ANIHAL NUMBER: 9OA3014 SEX: MALE DOSE: 200 PPM TEST MATERIAL: MODE OF DEATH: NECROPSY DATE: 1, 1,1-T SACRIFICED 09/13/90 (DAY 101) FINAL BODY WEIGHT (GRAMS): 293.6 ORGAN WEIGHTS (GRAMS): BRAIN 1.B21 GROSS PATHOLOGIC OBSERVATIONS (COMPLETE NECROPSY PERFORMED): `ALL TISSUES HERE EXAMINED AND WERE WITHIN NORMAL LIMITS* t END OF REPORT FOR ANIMAL 90A3814 t DURATION: ROUTE: SPECIES: 13-WEEK INHALATION RAT ANIMAL NUMBER: 90A3B15 SEX: MALE DOSE: 200 FINAL BODY WEIGHT (GRAMS): 321.0 PPM TEST MATERIAL: MODE OF OEATH: NECROPSY DATE: 1.1, 1-T SACRIFICED 10/16/90 (DAY 134) GROSS PATHOLOGIC OBSERVATIONS (COMPLETE NECROPSY PERFORMED): *ALL TISSUES HERE EXAMINED AND WERE WITHIN NORMAL LIMITS* I END OF REPORT FOR ANIMAL 90A3B15 t DURATION: ROUTE: SPECIES: 13-WEEK INHALATION RAT tSOX 1,1,1-T subchronic neurotoxicity APPENDIX Q: GROSS PATHOLOGY, GROUP B The Dow Chemical Company Study ID: K-001716-091 Volume 3 of 3, Page: 37 ANIMAL NUMBER: 90A3B16 SEX: MALE DOSE: 200 PPM TEST MATERIAL: MODE OF DEATH: NECROPSY DATE: 1,1, 1-T SACRIFICED 09/06/90 (DAY 94) DURATION: ROUTE: SPECIES: 13-WEEK INHALATION RAT GROSS PATHOLOGIC OBSERVATIONS (COMPLETE NECROPSY PERFORMED): EXTERNAL AND SKIN PERINEAL SOILING HEMORRHAGE, HEAD COMMENT: THE HEMORRHAGE RESULTED FROM LOSS OF THE EPIDURAL ELECTRODES. GENERAL COMMENT THE ANIMAL HAS SACRIFICED PRIOR TO THE STUDY TERMINATION DUE TO THE LOSS OF THE EPIDURAL ELECTRODES. ALL OTHER TISSUES HERE HITHIN NORMAL LIMITS I END OF REPORT FOR ANIMAL 9OA3016 t ANIMAL NUMBER: 90A3817 SEX: MALE DOSE: 200 FINAL BODY HEIGHT (GRAMS): 294.5 PPM TEST MATERIAL: MODE OF DEATH: NECROPSY DATE: 1,1,I-T SACRIFICED 10/16/90 (DAY 134) GROSS PATHOLOGIC OBSERVATIONS (COMPLETE NECROPSY PERFORMED): *ALL TISSUES HERE EXAMINED AND HERE HITHIN NORMAL LIMITS* I END OF REPORT FOR ANIMAL 90A3817 I DURATION: ROUTE: SPECIES: 13-WEEK INHALATION RAT r 1,1,1-T subchronic neurotoxicity APPENDIX Q: GROSS PATHOLOGY, GROUP B The Dow Chemical Company Study ID: K-001716-091 Volume 3 of 3, Page: 38 ANIMAL NUMBER: 90A3818 SEX: MALE DOSE: 200 FINAL BODY WEIGHT (GRAMS): 320.8 PPM TEST MATERIAL: MODE OF DEATH: NECROPSY DATE: 1,1,1-T SACRIFICED 10/16/90 (DAY 134) DURATION: ROUTE: SPECIES: 13-WEEK INHALATION RAT GROSS PATHOLOGIC OBSERVATIONS (COMPLETE NECROPSY PERFORMED): `ALL TISSUES MERE EXAMINED AND HERE HITHIN NORMAL LIMITS* I END OF REPORT FOR ANIMAL 90A3818 t SL 031084 ANIMAL NUMBER: 90A3819 SEX: MALE DOSE: 200 FINAL BODY HEIGHT (GRAMS): 308.0 PPM TEST MATERIAL: MODE OF DEATH: NECROPSY DATE: 1,1,1-T SACRIFICED 09/13/90 (DAY 101) ORGAN HEIGHTS (GRAMS): BRAIN 1.383 GROSS PATHOLOGIC OBSERVATIONS (COMPLETE NECROPSY PERFORMED): `ALL TISSUES HERE EXAMINED AND HERE HITHIN NORMAL LIMITS* I END OF REPORT FOR ANIMAL 90A3B19 I DURATION: ROUTE: SPECIES: 13-WEEK INHALATION RAT 1,1,1-T subchronic neurotoxicity APPENDIX Q: GROSS PATHOLOGY, GROUP fl The Dow Chemical Company Study ID: K-001716-091 Volume 3 of 3, Page: 39 ANIMAL NUMBER: 90A3827 SEX: MALE DOSE: 630 PPM TEST MATERIAL: MODE OF DEATH: NECROPSY DATE: 1,1,1-T SPONTANEOUS 08/06/90 (DAY 63) DURATION: ROUTE: SPECIES: 13-WEEK INHALATION RAT GROSS PATHOLOGIC OBSERVATIONS (COMPLETE NECROPSY PERFORMED): U! t* GENERAL CONGESTION, VISCERA LARYNX MUCUS, LUMEN LUNGS ATELECTASIS, BILATERAL, MULTIFOCAL GENERAL COMMENT THE CAUSE OF DEATH APPEARS TO BE RELATED TO DIFFICULT BREATHING. ALL OTHER TISSUES HERE WITHIN NORMAL LIMITS I END OF REPORT FOR ANIMAL 90A3827 I ANIMAL NUMBER: 90A3828 SEX: MALE DOSE: 630 FINAL BODY WEIGHT (GRAMS): 365.7 PPM TEST MATERIAL: MODE OF DEATH: NECROPSY DATE; 1,1,1-T SACRIFICED 10/16/90 (DAY 134) GROSS PATHOLOGIC OBSERVATIONS (COMPLETE NECROPSY PERFORMED): *ALL TISSUES HERE EXAMINED AND WERE WITHIN NORMAL LIMITS* t END OF REPORT FOR ANIMAL 90A3828 t DURATION: ROUTE: SPECIES: 13-WEEK INHALATION RAT 80t 1E0* O' 1,1,1-T subchronic neurotoxicity APPENDIX Q: GROSS PATHOLOGY, GROUP B The Dow Chemical Company Study ID: K-001716-091 Volume 3 of 3, Page: 40 ANIMAL NUMBER: 90A3829 SEX: MALE DOSE: 630 FINAL BODY HEIGHT (GRAMS): 331.1 PPM TEST MATERIAL: MODE OF DEATH: NECROPSY DATE: 1.1,1-T SACRIFICED 10/16/90 (DAY 134) DURATION; ROUTE: SPECIES: 13-WEEK INHALATION RAT GROSS PATHOLOGIC OBSERVATIONS (COMPLETE NECROPSY PERFORMED): `ALL TISSUES HERE EXAMINED AND HERE WITHIN NORMAL LIMITS* I END OF REPORT FOR ANIMAL 90A3829 t ANIMAL NUMBER: 90A3830 SEX: MALE DOSE: 630 FINAL BODY HEIGHT (GRAMS): 261.0 PPM TEST MATERIAL: MODE OF DEATH: NECROPSY DATE: 1, 1,1-T SACRIFICED 09/13/90 (DAY 101) ORGAN HEIGHTS (GRAMS): BRAIN 1.309 GROSS PATHOLOGIC OBSERVATIONS (COMPLETE NECROPSY PERFORMED): `ALL TISSUES HERE EXAMINED AND HERE WITHIN NORMAL LIMITS* I END OF REPORT FOR ANIMAL 90A3B30 I DURATION: ROUTE: SPECIES; 13-WEEK INHALATION RAT 1,1,1 -T subchronic neurotoxicity APPENDIX Q: GROSS PATHOLOGY, GROUP B The Dow Chemical Company Study ID: K-001716-091 Volume 3 of 3, Page: 41 ANIMAL NUMBER: 90A3831 SEX: MALE DOSE: 630 PPM TEST MATERIAL: MODE OF DEATH: NECROPSY DATE: 1,1,1-T SACRIFICED 10/10/90 (DAY 128) DURATION: ROUTE: SPECIES: 13-WEEK INHALATION RAT U) r* GROSS PATHOLOGIC OBSERVATIONS (COMPLETE NECROPSY PERFORMED): 031087 *ALL TISSUES HERE EXAMINED AND WERE WITHIN NORMAL LIMITS* GENERAL COMMENT THE ANIMAL WAS SACRIFICED PRIOR TO STUDY TERMINATION DUE TO LOSS OF THE ACRYLIC CAP. I END OF REPORT FOR ANIMAL 90A3831 I ANIMAL NUMBER: 90A3832 SEX: MALE DOSE: 630 FINAL BODY WEIGHT (GRAMS): 331.7 PPM TEST MATERIAL: MODE OF DEATH: NECROPSY DATE: 1,1,1-T SACRIFICED 10/16/90 (DAY 131) GROSS PATHOLOGIC OBSERVATIONS (COMPLETE NECROPSY PERFORMED): *ALL TISSUES WERE EXAMINED AND HERE WITHIN NORMAL LIMITS* t END OF REPORT FOR ANIMAL 90A3832 I DURATION: ROUTE: SPECIES: 13-WEEK INHALATION RAT 1,1,1-T subchronic neurotoxicity APPENDIX Q: GROSS PATHOLOGY, GROUP B The Dow Chemical Company Study ID: K-001716-091 Volume 3 of 3, Page: 42 ANIMAL NUMBER: 90A3833 SEX: MALE DOSE: 630 FINAL BODY WEIGHT (GRAMS): 325.7 PPM TEST MATERIAL; MODE OF DEATH: NECROPSY DATE: 1,1,1-T SACRIFICED 09/13/90 (DAY 101) ORGAN HEIGHTS (GRAMS): BRAIN 1.896 GROSS PATHOLOGIC OBSERVATIONS (COMPLETE NECROPSY PERFORMED): `ALL TISSUES WERE EXAMINED AND WERE WITHIN NORMAL LIMITS* I END OF REPORT FOR ANIMAL 90A3833 I DURATION: ROUTE: SPECIES: 13-WEEK INHALATION RAT SL 031088 ANIMAL NUMBER: 90X3811 SEX: MALE DOSE: 2000 FINAL BODY HEIGHT (GRAMS): 328.5 PPM TEST MATERIAL: MODE OF DEATH: NECROPSY DATE: 1,1,I-T SACRIFICED 09/13/90 (DAY 101) ORGAN WEIGHTS (GRAMS): BRAIN 1.809 GROSS PATHOLOGIC OBSERVATIONS (COMPLETE NECROPSY PERFORMED): `ALL TISSUES HERE EXAMINED AND HERE WITHIN NORMAL LIMITS* I END OF REPORT FOR ANIMAL 90A3841 I DURATION; ROUTE: SPECIES: 13-WEEK INHALATION RAT 1,1,1-T subchronic neurotoxicity APPENDIX G: GROSS PATHOLOGY, GROUP B The Dow Chemical Company Study ID: K-001716-091 Volume 3 of 3, Page: 43 ANIMAL NUMBER: 90A3842 SEX: MALE DOSE: 2000 (INAL BODY WEIGHT (GRAMS): 360.6 PPM TEST MATERIAL: MODE OF DEATH: NECROPSY DATE: 1,1,1-T SACRIFICED 10/16/90 (DAY 134) DURATION: ROUTE: SPECIES: 13-WEEK INHALATION RAT GROSS PATHOLOGIC OBSERVATIONS (COMPLETE NECROPSY PERFORMED): *ALL TISSUES WERE EXAMINED AND WERE WITHIN NORMAL LIMITS* I END OF REPORT FOR ANIMAL 90A3842 I SL 031089 ANIMAL NUMBER: 90A3843 SEX: MALE DOSE: 2000 FINAL BODY HEIGHT (GRAMS): 286.7 PPM TEST MATERIAL: MODE OF DEATH: NECROPSY DATE: 1,1,1-T SACRIFICED 10/16/90 (DAY 134) GROSS PATHOLOGIC OBSERVATIONS (COMPLETE NECROPSY PERFORMED): EXTERNAL AND SKIN ABSCESS, HEAD, FOCAL COMMENT: THERE IS PURULENT EXUDATE UNDERLYING AND SURROUNDING THE BASE OF THE ACRYLIC CAP. THE EXUDATE IS BETWEEN THE DERMIS AND CALVARIAL BONE. ALL OTHER TISSUES WERE WITHIN NORMAL LIMITS I END OF REPORT FOR ANIMAL 90A3843 I DURATION: ROUTE: SPECIES: 13-WEEK INHALATION RAT 10g0 1,1,1-T subchronic neurotoxicity APPENDIX Q: . . OSS PATHOLOGY, GROUP B The Dow Chemical Company Study ID: K-001716-091 Volume 3 of 3, Page: 44 ^ O tu ANIMAL NUMBER; 90A3844 SEX: MALE DOSE: 2000 PPM TEST MATERIAL: MODE OP DEATH: NECROPSY DATE: 1, 1, 1-T SACRIFICED 09/13/90 (DAY 101) PINAL BODY HEIGHT (GRAMSI : 331.7 ORGAN HEIGHTS (GRAMS): BRAIN 1.853 GROSS PATHOLOGIC OBSERVATIONS (COMPLETE NECROPSY PERPORMED): *ALL TISSUES WERE EXAMINED AND WERE HITIIIN NORMAL LIMITS* t END OP REPORT FOR ANIMAL 90A3844 t DURATION: ROUTE: SPECIES: 13-WEEK INHALATION RAT ANIMAL NUMBER: 90A3845 SEX: MALE DOSE: 2000 PPM TEST MATERIAL: MODE OF DEATH: NECROPSY DATE: 1,1,1-T SACRIFICED 09/13/90 (DAY 101) FINAL BODY WEIGHT (GRAMSI: 306.7 ORGAN WEIGHTS (GRAMS): BRAIN 1.928 GROSS PATHOLOGIC OBSERVATIONS (COMPLETE NECROPSY PERFORMED): ALL TISSUES WERE EXAMINED AND WERE WITHIN NORMAL LIMITS* t END OF REPORT FOR ANIMAL 90A3845 I DURATION: ROUTE: SPECIES: 13-WEEK INHALATION RAT 1 1,1,1 -T subchronic neurotoxicity APPENDIX Q: GROSS PATHOLOGY, GROUP B The Dow Chemical Company Study ID: K-001716-091 Volume 3 of 3, Page: 45 ANIMAL NUMBER: 90A3846 SEX: MALE DOSE: 2000 PPM TEST MATERIAL: MODE OF DEATH: NECROPSY DATE: 1,1,l-T SPONTANEOUS 08/02/90 (DAT 59) DURATION: ROUTE: SPECIES: 13-WEEK INHALATION RAT U) GROSS PATHOLOGIC OBSERVATIONS (COMPLETE NECROPSY PERFORMED): 031091 GENERAL CONGESTION, VISCERA GENERAL COMMENT THE CAUSE OF DEATH HAS NOT DETERMINED. ALL OTHER TISSUES WERE WITHIN NORMAL LIMITS I END OF REPORT FOR ANIMAL 90A3846 I ANIMAL NUMBER: 90A3847 SEX: MALE DOSE: 2000 FINAL BODY WEIGHT (GRAMS|: 329.1 PPM TEST MATERIAL: MODE OF DEATH: NECROPSY DATE; 1. 1,l-T SACRIFICED 10/16/90 (DAY 134) GROSS PATHOLOGIC OBSERVATIONS (COMPLETE NECROPSY PERFORMED): `ALL TISSUES WERE EXAMINED AND HERE WITHIN NORMAL LIMITS* I END OF REPORT FOR ANIMAL 90A3847 I DURATION: ROUTE: SPECIES: 1 3-WEEK INHALATION RAT 1,1,1-T subchronic neurotoxicity APPENDIX Q: GROSS PATHOLOGY, GROUP B The Dow Chemical Company Study ID: K-001716-091 Volume 3 of 3, Page: 46 SL 031092 ANIMAL NUMBER: 90A3855 SEX: FEMALE DOSE: 0 PPM FINAL BODY WEIGHT (GRAHS): 175.0 TEST MATERIAL: MODE OF DEATH: NECROPSY DATE: 1,1,1-T SACRIFICED 09/13/90 (DAY 101) ORGAN WEIGHTS (GRAMS): BRAIN 1.721 GROSS PATHOLOGIC OBSERVATIONS (COMPLETE NECROPSY PERFORMED): `ALL TISSUES HERE EXAMINED AND WERE WITHIN NORMAL LIMITS* I END OF REPORT FOR ANIMAL 90A38S5 t DURATION: ROUTE: SPECIES: 13-WEEK INHALATION RAT ANIMAL NUMBER: 9OA3056 SEX: FEMALE DOSE: 0 PPM FINAL BODY WEIGHT (GRAMS): 174.6 TEST MATERIAL: MODE OF DEATH: NECROPSY DATE: 1, 1,1-T SACRIFICED 09/13/90 (DAY 101) ORGAN WEIGHTS (GRAMS): BRAIN 1.600 GROSS PATHOLOGIC OBSERVATIONS (COMPLETE NECROPSY PERFORMED): `ALL TISSUES HERE EXAMINED AND WERE WITHIN NORMAL LIMITS* I END OF REPORT FOR ANIMAL 9OA3056 I DURATION; ROUTE: SPECIES: 13-WEEK INHALATION RAT 1,1,1 -T subchronic neurotoxicity APPENDIX Q: GROSS PATHOLOGY, GROUP B The Dow Chemical Company Study ID: K-001716-091 Volume 3 of 3, Page: 47 ANIMAL NUMBER: 90A38S7 SEX: FEMALE DOSE: 0 PPM FINAL BODY HEIGHT (GRAMS): 169.8 TEST MATERIAL: MODE OF DEATH: NECROPSY DATE: 1,1,1-T SACRIFICED 09/13/90 (DAY 101) DURATION: ROUTE: SPECIES: 13-WEEK INHALATION RAT yj ORGAN WEIGHTS (GRAMS): e* BRAIN 1. 708 031093 GROSS PATHOLOGIC OBSERVATIONS (COMPLETE NECROPSY PERFORMED): ALL TISSUES HERE EXAMINED AND HERE WITHIN NORMAL LIMITS* I END OF REPORT FOR ANIMAL 90A3B57 I ANIMAL NUMBER: 90A38S8 SEX; FEMALE DOSE: 0 PPM TEST MATERIAL: MODE OF DEATH: NECROPSY DATE: 1,1,1-T SACRIFICED 09/06/90 (DAY 94) DURATION: ROUTE: SPECIES: 13-WEEK INHALATION RAT GROSS PATHOLOGIC OBSERVATIONS (COMPLETE NECROPSY PERFORMED!: EXTERNAL AND SKIN PERINEAL SOILING HEMORRHAGE, HEAD COMMENT: THE HEMORRHAGE RESULTED FROM LOSS OF THE EPIDURAL ELECTRODES. GENERAL COMMENT THE ANIMAL HAS SACRIFICED PRIOR TO STUDY TERMINATION DUE TO THE LOSS OF THE EPIDURAL ELECTRODES. ALL OTHER TISSUES WERE WITHIN NORMAL LIMITS t END OF REPORT FOR ANIMAL 90A38S8 I 1,1,1-T subchronic neurotoxicity APPENDIX Q: GROSS PATHOLOGY, GROUP B Hie Dow Chemical Company Study ID: K-001716-091 Volume 3 of 3, Page: 48 ANIMAL NUMBER: 90A3859 SEX; FEMALE DOSE: 0 PPM TEST MATERIAL: MODE OF DEATH: NECROPSY DATE: 1,1,1-T SACRIFICED 10/03/90 (DAY 121) DURATION: ROUTE: SPECIES: 13-WEEK INHALATION RAT GROSS PATHOLOGIC OBSERVATIONS (COMPLETE NECROPSY PERFORMED): UTERUS STRANGULATED OR NECROTIC FAT, FOCAL w GENERAL COMMENT THE ANIMAL HAS SACRIFICED PRIOR TO STUDY TERMINATION DUE TO LOSS OF THE EPIDURAL ELECTRODES. ALL OTHER TISSUES HERE HITHIN NORMAL LIMITS t END OF REPORT FOR ANIMAL 90A3859 I 60X ANIMAL NUMBER: 90A38 60 SEX: FEMALE DOSE: 0 PPM FINAL BODY HEIGHT (GRAMS): 170.2 TEST MATERIAL: MODE OF DEATH: NECROPSY DATE: 1, 1,1-T SACRIFICED 10/16/90 (DAY 1341 DURATION: ROUTE: SPECIES: 13-WEEK INHALATION RAT GROSS PATHOLOGIC OBSERVATIONS (COMPLETE NECROPSY PERFORMED): *ALL TISSUES HERE EXAMINED AND HERE HITHIN NORMAL LIMITS* I END OF REPORT FOR ANIMAL 90A3860 I 1,1,1-T subchronic neurotoxicity APPENDIX Q: GROSS PATHOLOGY, GROUP B The Dow Chemical Company Study ID: K-001716-091 Volume 3 of 3, Page: 49 ANIMAL NUMBER: 90A3861 SEX: FEMALE DOSE: 0 PPM TEST MATERIAL: MODE OF DEATH: NECROPSY DATE: 1, 1, 1-T SACRIFICED 10/16/90 (DAY 134) DURATION: ROUTE: SPECIES: 13-WEEK INHALATION RAT FINAL BODY HEIGHT (GRAMS): 168.4 cn f GROSS PATHOLOGIC OBSERVATIONS (COMPLETE NECROPSY PERFORMED): 031095 EYES OPACITY, LENS, LEFT ALL OTHER TISSUES HERE WITHIN NORMAL LIMITS I END OF REPORT FOR ANIMAL 9OA3061 I ANIMAL NUMBER: 9GA3B69 SEX: FEMALE DOS E: 200 PPM FINAL BODY WEIGHT (GRAMS): 171.5 TEST MATERIAL: MODE OF DEATH: NECROPSY DATE: 1,1,1-T SACRIFICED 09/13/90 (DAY 101) ORGAN HEIGHTS (GRAMS): BRAIN 1.757 GROSS PATHOLOGIC OBSERVATIONS (COMPLETE NECROPSY PERFORMED): *ALL TISSUES HERE EXAMINED AND HERE WITHIN NORMAL LIMITS* I END OF REPORT FOR ANIMAL 90A3869 I DURATION: ROUTE: SPECIES: 13-WEEK INHALATION RAT 1,1,1-T subchronic neurotoxicity APPENDIX Q: GROSS PATHOLOGY, GROUP B The Dow Chemical Company Study ID: K-001716-091 Volume 3 of 3, Page: 50 ANIMAL NUMBER: 90A3870 SEX: FEMALE DOSE: 200 PPM FINAL BODY HEIGHT (GRAMS): 174.8 TEST MATERIAL: MODE OF DEATH: NECROPSY DATE: 1,1,1-T SACRIFICED 10/16/90 {DAY 134] DURATION: ROUTE: SPECIES: 13-WEEK INHALATION RAT GROSS PATHOLOGIC OBSERVATIONS (COMPLETE NECROPSY PERFORMED): ALL TISSUES HERE EXAMINED AND WERE WITHIN NORMAL LIMITS* I END OF REPORT FOR ANIMAL 90A3870 I SL 031096 I 1,1,1-T subchronic neurotoxicity APPENDIX Q: GROSS PATHOLOGY, GROUP B The Dow Chemical Company Study ID: K-001716-091 Volume 3 of 3, Page: 51 ANIMAL NUMBER: 90A387I SEX: FEMALE DOSE: 200 PPM TEST MATERIAL: MODE OF DEATH: NECROPSY DATE: 1.1.1-T SACRIFICED 09/05/90 (DAY 93) DURATION: ROUTE: SPECIES: 13-WEEK INHALATION RAT Z.60I0 GROSS PATHOLOGIC OBSERVATIONS (COMPLETE NECROPSY PERFORMED): BONE FRACTURE - RECENT, CRANIAL CAVITY yj COMMENT: A 4MM X 4MM PIECE OF THE RIGHT PARIETAL BONE, HAS t* TRAUMATICALLY SEPARATED FROM THE SKULL DURING LOSS OF THE EPIDURAL ELECTRODES. BRAIN HEMOHRHAGE, CEREBRUM, RIGHT, FOCAL EXTERNAL AND SKIN HEMORRHAGE, HEAD GENERAL COMMENT THE ANIMAL HAS SACRIFICED PRIOR TO STUDY TERMINATION DUE TO LOSS OF THE EPIDURAL ELECTRODES. ALL LESIONS HERE THE RESULT OF TRAUMA ASSOCIATED HITH LOSS OF THE EPIDURAL ELECTRODES. ALL OTHER TISSUES HERE HITHIN NORMAL LIMITS I END OF REPORT FOR ANIMAL 90A3a?l t 1,1,1-T subchronic neurotoxicity APPENDIX Q: GROSS PATHOLOGY, GROUP B The Dow Chemical Company Study ID: K-001716-091 Volume 3 of 3, Page: 52 ANIMAL NUMBER: 90A3872 SEX: FEMALE DOSE: 200 PPM FINAL BODY WEIGHT (GRAMS): 171.3 TEST MATERIAL: MODE OF DEATH: NECROPSY DATE: 1,1,1-T SACRIFICED 10/16/90 (DAY 134) DURATION: ROUTE: SPECIES: 13-WEEK INHALATION RAT GROSS PATHOLOGIC OBSERVATIONS (COMPLETE NECROPSY PERFORMED): ALL TISSUES HERE EXAMINED AND HERE WITHIN NORMAL LIMITS* I END OF REPORT FOR ANIMAL 90A3872 I 031098 ANIMAL NUMBER: 90A3873 SEX: FEMALE DOSE: 200 PPM FINAL BODY HEIGHT (GRAMS): 180.4 TEST MATERIAL: MODE OF DEATH: NECROPSY DATE: 1, 1, 1-T SACRIFICED 10/16/90 (DAY 134) DURATION: ROUTE: SPECIES: 13-WEEK INHALATION RAT GROSS PATHOLOGIC OBSERVATIONS (COMPLETE NECROPSY PERFORMED)i PREPUTIAL OR CLITORAL GLANDS ABSCESS, UNILATERAL ALL OTHER TISSUES HERE WITHIN NORMAL LIMITS I END OF REPORT FOR ANIMAL 90A3873 I 1,1,1-T subchronic neurotoxicity APPENDIX Q: GROSS PATHOLOGY, GROUP B The Dow Chemical Company Study ID: K-001716-091 Volume 3 of 3, Page: 53 ANIMAL NUMBER: 90A3874 SEX: FEMALE DOSE: 200 PPM TEST MATERIAL: MODE OF DEATH: NECROPSY DATE: 1,1,1-T SACRIFICED 10/16/90 (DAY 134) DURATION: ROUTE: SPECIES; 13-HEEK INHALATION RAT FINAL BODY HEIGHT (GRAMS(: 171.5 U) GROSS PATHOLOGIC OBSERVATIONS (COMPLETE NECROPSY PERFORMED): `ALL TISSUES HERE EXAMINED AND HERE HI THIN NORMAL LIMITS* I END OF REPORT FOR ANIMAL 90A3874 f 031099 ANIMAL NUMBER: 90A3S7S SEX: FEMALE DOSE: 200 PPM FINAL BODY HEIGHT (GRAMS): 177.4 TEST MATERIAL: MODE OF DEATH: NECROPSY DATE: l, 1- 1-T SACRIFICED 09/13/90 (DAY 101) ORGAN HEIGHTS (GRAMS): BRAIN 1.725 GROSS PATHOLOGIC OBSERVATIONS (COMPLETE NECROPSY PERFORMED); *ALL TISSUES HERE EXAMINED AND HERE HITHIN NORMAL LIMITS* I END OF REPORT FOR ANIMAL 90A3875 I DURATION: ROUTE: SPECIES: 13-HEEK INHALATION RAT ] 1,1,1-T subchronic neurotoxicity APPENDIX Q: GROSS PATHOLOGY, GROUP B The Dow Chemical Company Study ID: K-001716-091 Volume 3 of 3, Page: 54 ANIMAL NUMBER: 90A3883 SEX: FEMALE DOSE: 630 PPM TEST MATERIAL: MODE OF DEATH: NECROPSY DATE: 1,1, 1-T SACRIFICED 09/13/90 (DAY 101) FINAL BODY HEIGHT (GRAMS): 161.0 ORGAN WEIGHTS (GRAMS): BRAIN 1.123 GROSS PATHOLOGIC OBSERVATIONS (COMPLETE NECROPSY PERFORMED): `ALL TISSUES WERE EXAMINED AND WERE WITHIN NORMAL LIMITS* I END OF REPORT FOR ANIMAL 90A3883 t DURATION: ROUTE: SPECIES: 13-WEEK INHALATION RAT ANIMAL NUMBER: 90A3884 SEX: FEMALE DOSE: 630 PPM FINAL BODY HEIGHT (GRAMS): 179.2 TEST MATERIAL: MODE OF DEATH: NECROPSY DATE: 1,1,1-T SACRIFICED 10/16/90 (DAY 134) GROSS PATHOLOGIC OBSERVATIONS (COMPLETE NECROPSY PERFORMED): *ALL TISSUES WERE EXAMINED AND HERE WITHIN NORMAL LIMITS* I END OF REPORT FOR ANIMAL 90A3B84 I DURATION; ROUTE: SPECIES: 13-WEEK INHALATION RAT SL 031100 1,1,1-T subchronic neurotoxicity APPENDIX 0: GROSS PATHOLOGY, GROUP B The Dow Chemical Company Study ID: K-001716-091 Volume 3 of 3, Page: 55 ANIMAL NUMBER: 90A3685 SEX: FEMALE DOSE: 630 PPM FINAL BODY WEIGHT (GRAMS): 163.1 TEST MATERIAL: MODE OF DEATH: NECROPSY DATE: 1,1,1-T SACRIFICED 09/13/90 (DAY 101) DURATION: ROUTE: SPECIES: 13-WEEK INHALATION RAT ORGAN WEIGHTS (GRAMS): Crt BRAIN f 1.684 031101 GROSS PATHOLOGIC OBSERVATIONS (COMPLETE NECROPSY PERFORMED): `ALL TISSUES WERE EXAMINED AND WERE WITHIN NORMAL LIMITS* I END OF REPORT FOR ANIMAL 90A3865 I ANIMAL NUMBER: 90A3886 SEX: FEMALE DOSE: 630 PPM FINAL BODY WEIGHT (GRAMS): 158.3 TEST MATERIAL: MODE OF DEATH: NECROPSY DATE: 1, 1, 1-T SACRIFICED 09/13/90 (DAY 101) ORGAN HEIGHTS (GRAMS): BRAIN 1.665 GROSS PATHOLOGIC OBSERVATIONS (COMPLETE NECROPSY PERFORMED): `ALL TISSUES HERE EXAMINED AND HERE WITHIN NORMAL LIMITS* I END OF REPORT FOR ANIMAL 90A3886 I DURATION: ROUTE: SPECIES: 13-WEEK INHALATION RAT 1,1,1-T subchronic neurotoxicity APPENDIX Q: GROSS PATHOLOGY, GROUP B The Dow Chemical Company Study ID: K-001716-091 Volume 3 of 3, Page: 56 ANIMAL NUMBER: 90A38B7 SEX: FEMALE DOSE: 630 PPM PINAL BODY WEIGHT (GRAMS): 111.1 TEST MATERIAL: MODE OF DEATH: NECROPSY DATE: 1.1,1-T SACRIFICED 10/16/90 (DAY 134) DURATION: ROUTE: SPECIES: 13-WEEK INHALATION RAT GROSS PATHOLOGIC OBSERVATIONS tCOMPLETE NECROPSY PERFORMED): * ALL TISSUES MERE EXAMINED AND HERE WITHIN NORMAL LIMITS* t END OF REPORT FOR ANIMAL 90A3887 I SL 031102 ANIMAL NUMBER: 90A3888 SEX: FEMALE DOSE: 630 PPM FINAL BODY HEIGHT (GRAMS): 183.5 TEST MATERIAL: MODE OF DEATH: NECROPSY DATE: 1,1,1-T SACRIFICED 10/16/90 (DAY 134) GROSS PATHOLOGIC OBSERVATIONS (COMPLETE NECROPSY PERFORMED) : `ALL TISSUES HERE EXAMINED AND HERE WITHIN NORMAL LIMITS* t END OF REPORT FOR ANIMAL 9QA3888 I DURATION: ROUTE: SPECIES: 13-WEEK INHALATION RAT 1,1,1-T subchronic neurotoxicity APPENDIX Q: GROSS PATHOLOGY, GROUP B The Dow Chemical Company Study ID: K-001716-091 Volume 3 of 3, Page: 57 ANIMAL NUMBER: 9OA3809 SEX: FEMALE DOSE: 630 PPM FINAL BODY WEIGHT (GRAMS): 177.0 C/1 TEST MATERIAL: MODE OF DEATH: NECROPSY DATE: 1,1,1-T SACRIFICED 10/16/90 (DAY 134) DURATION: ROUTE: SPECIES: 13-WEEK INHALATION RAT O it GROSS PATHOLOGIC OBSERVATIONS (COMPLETE NECROPSY PERFORMED); *ALL TISSUES WERE EXAMINED AND WERE WITHIN NORMAL LIMITS* I END OF REPORT FOR ANIMAL 9OA3089 I ANIMAL NUMBER: 90A3897 SEX: FEMALE DOSE: 2000 PPM FINAL BODY HEIGHT (GRAMS): 169.0 TEST MATERIAL: MODE OF DEATH: NECROPSY DATE: 1,1,1-T SACRIFICED 09/13/90 (DAY 101) ORGAN WEIGHTS (GRAMS): BRAIN 1.697 GROSS PATHOLOGIC OBSERVATIONS (COMPLETE NECROPSY PERFORMED): `ALL TISSUES WERE EXAMINED AND WERE WITHIN NORMAL LIMITS* I END OF REPORT FOR ANIMAL 90A3897 I DURATION: ROUTE: SPECIES: 13-WEEK INHALATION RAT 1,1,1-T subchronic neurotoxicity APPENDIX Q: GROSS PATHOLOGY, GROUP B The Dow Chemical Company Study ID: K-001716-091 Volume 3 of 3, Page: 58 ANIMAL NUMBER: 90A3898 SEX: FEMALE DOSE: 2000 PPM PINAL BODY WEIGHT (GRAMS): 176.2 TEST MATERIAL: MODE OF DEATH: NECROPSY DATE: 1,1,1-T SACRIFICED 10/16/90 (DAY 134) DURATION: ROUTE: SPECIES: 13-WEEK INHALATION RAT GROSS PATHOLOGIC OBSERVATIONS (COMPLETE NECROPSY PERFORMED): ALL TISSUES HERE EXAMINED AND WERE WITHIN NORMAL LIMITS* I END OF REPORT FOR ANIMAL 90A3898 I SL 031104 ANIMAL NUMBER: 90A3899 SEX: FEMALE DOSE: 2000 PPM FINAL BODY HEIGHT (GRAMS); 174.4 TEST MATERIAL: MODE OF DEATH: NECROPSY DATE: 1,1,1-T SACRIFICED 10/16/90 (DAY 134) GROSS PATHOLOGIC OBSERVATIONS (COMPLETE NECROPSY PERFORMED): URINARY BLADDER CALCULUS(I), LUMEN THICKENED, HALL COMMENT: A LARGE CALCULUS FILLS THE BLADDER LUMEN. THE THICKENED BLADDER HALL IS SECONDARY TO THE CALCULUS. ALL OTHER TISSUES WERE WITHIN NORMAL LIMITS I END OF REPORT FOR ANIMAL 90A3899 I DURATION: ROUTE: SPECIES: 13-WEEK INHALATION RAT 1,1,1-T subchronic neurotoxicity APPENDIX Q: GROSS PATHOLOGY, GROUP B The Dow Chemical Company Study ID: K-001716-091 Volume 3 of 3, Page: 59 ANIMAL NUMBER: 90A3900 SEX: FEMALE DOSE: 2000 PPM FINAL BODY WEIGHT (GRAMS): 170.5 TEST MATERIAL: MODE OF DEATH: NECROPSY DATE: 1,1,1-T SACRIFICED 10/16/90 (DAY 134) DURATION: ROUTE: SPECIES: 13-WEEK INHALATION RAT GROSS PATHOLOGIC OBSERVATIONS (COMPLETE NECROPSY PERFORMED): `ALL TISSUES WERE EXAMINED AND WERE WITHIN NORMAL LIMI.TS* I END OF REPORT FOR ANIMAL 90A3900 I S O It 1S ANIMAL NUMBER: 90A3901 SEX: FEMALE DOSE: 2000 PPM FINAL BODY HEIGHT (GRAMS): 172.1 TEST MATERIAL: MODE OF DEATH: NECROPSY DATE: 1,1,1-T SACRIFICED 09/13/90 (DAY 101) ORGAN WEIGHTS (GRAMS): BRAIN 1.701 GROSS PATHOLOGIC OBSERVATIONS (COMPLETE NECROPSY PERFORMED): `ALL TISSUES WERE EXAMINED AND WERE WITHIN NORMAL LIMITS* I END OF REPORT FOR ANIMAL 90A3901 t DURATION: ROUTE: SPECIES: 13-WEEK INHALATION RAT 1,1,1-T subchronic neurotoxicity APPENDIX Q: GROSS PATHOLOGY, GROUP B The Dow Chemical Company Study ID: K-001716D91 Volume 3 of 3, Page: 60 ANIMAL NUMBER: 90A3902 SEX: FEMALE DOSE: 2000 PPM m FINAL BODY HEIGHT (GRAMS): 172.3 r* TEST MATERIAL: MODE OF DEATH: NECROPSY DATE: 1, 1, 1-T SACRIFICED 10/16/90 (DAY 134) DURATION: ROUTE: SPECIES: 13-HEEK INHALATION RAT 9 0 X t0 GROSS PATHOLOGIC OBSERVATIONS (COMPLETE NECROPSY PERFORMED): *ALL TISSUES HERE EXAMINED AND HERE HITHIN NORMAL LIMITS* I END OF REPORT FOR ANIMAL 90A3902 I ANIMAL NUMBER: 90A3903 SEX: FEMALE DOSE: 2000 PPM FINAL BODY HEIGHT (GRAMS): 178.2 TEST MATERIAL: MODE OF DEATH: NECROPSY DATE: 1,1,1-T SACRIFICED 10/16/90 (DAY 134) GROSS PATHOLOGIC OBSERVATIONS (COMPLETE NECROPSY PERFORMED): `ALL TISSUES HERE EXAMINED AND HERE HITHIN NORMAL LIMITS* I END OF REPORT FOR ANIMAL 90A3903 I DURATION; ROUTE: SPECIES: 13-HEEK INHALATION RAT 1,1,1-T subchronic neurotoxicity APPENDIX ft: NEUROPATHOLOGY, MALE The Dow Chemical Company Study ID: K-001716-091 Volume 3 of 3, Page: 61 ANIMAL NUMBER: 90A3792 SEX: MALE DOSE; 0 PPM TEST MATERIAL: MODE OF DEATH: NECROPSY DATE: 1,1,1-T SACRIFICED 05/29/90 (DAY 1 END OF REPORT FOR ANIMAL 90A3792 1 1) DURATION ROUTE SPECIES 19-WEEK INHALATION RAT SL 031107 ANIMAL NUMBER: 90A3793 SEX: MALE DOSE: 0 PPM TEST MATERIAL: MODE OF DEATH: NECROPSY DATE: 1, 1,1-T SACRIFICED 05/29/90 (DAY 1) DURATION ROUTE SPECIES 19-WEEK INHALATION RAT HISTOPATHOLOGIC OBSERVATIONS: GENERAL COMMENT THE SPECIAL STAINS Of SILVER AND CRESYL ECHT VIOLET-LFB-PAS HERE APPLIED TO EACH TISSUE HITH THE EXCEPTION OF EYES, OPTIC NERVE (WHICH IS WITH EYE) AND SKELETAL MUSCLE FROM EACH ANIMAL IN THIS GROUP. UNLESS OTHERWISE SPECIFIED, NO ABNORMALITIES HERE DETECTED IN THESE TISSUES USING THESE STAINS. THE FOLLOWING TISSUES HERE WITHIN NORMAL LIMITS FOR HISTOPATKOLOGY (EXCEPT FOR CHANGES RELATED TO ANY ABOVE OBSERVATIONS): BRAIN - CEREBELLUM, BRAIN - CEREBRUM, BRAIN - FOREBRAIN, BRAIN - MEDULLA OBLONGATA, BRAIN - MIDBRAIN, BRAIN - PONS, DORSAL ROOT GANGLIA, EYES, GASSERIAN GANGLIA, PERIPHERAL NERVE - OPTIC, PERIPHERAL NERVE - SCIATIC, PERIPHERAL NERVE - SURAL, PERIPHERAL NERVE - TIBIAL, SKELETAL MUSCLE, SPINAL CORD - CERVICAL, SPINAL CORD - LUMBAR, SPINAL NERVE ROOTS I END OF REPORT FOR ANIMAL 90A3793 I 1,1,1-T subchronic neurotoxicity APPENDIX R: NEUROPATHOLOGY, MALE The Dow Chemical Company Study ID: K-001716-091 Volume 3 of 3, Page; 62 ANIMAL NUMBER: 90A37 95 SEX: MALE DOSE: 0 PPM TEST MATERIAL: MODE OF DEATH: NECROPSY DATE: 1,1,1-T SACRIFICED 05/29/90 (DAY t END OP REPORT FOR ANIMAL 90A3795 t 1) DURATION: ROUTE: SPECIES: 19-WEEK INHALATION RAT gO X X ANIMAL NUMBER: 90A3796 SEX: MALE DOSE: 0 PPM HISTOPATHOLOGIC OBSERVATIONS: TEST MATERIAL: MODE OF DEATH: NECROPSY DATE: 1.1, 1-T SACRIFICED 05/29/90 (DAY 1) DURATION: ROUTE: SPECIES: 19-WEEK INHALATION RAT THE FOLLOWING TISSUES HERE WITHIN NORMAL LIMITS FOR HISTOPATHOLOGY (EXCEPT FOR CHANGES RELATED TO ANY ABOVE OBSERVATIONS): BRAIN - CEREBELLUM, BRAIN - CEREBRUM, BRAIN - FOREBRAIN, BRAIN - MEDULLA OBLONGATA, BRAIN - MIDBRAIN, BRAIN - PONS, DORSAL ROOT GANGLIA, EYES, GASSERIAN GANGLIA, PERIPHERAL NERVE - OPTIC, PERIPHERAL NERVE - SCIATIC, PERIPHERAL NERVE - SURAL, PERIPHERAL NERVE - TIBIAL, SKELETAL MUSCLE, SPINAL CORD - CERVICAL, SPINAL CORD - LUMBAR, SPINAL NERVE ROOTS I END OF REPORT FOR ANIMAL 90A3196 t ANIMAL NUMBER: 90A3797 SEX: MALE DOSE: 0 PPM TEST MATERIAL: MODE OF DEATH: NECROPSY DATE: 1,1,1-T SACRIFICED 05/29/90 (DAY I END OF REPORT FOR ANIMAL 90A3197 t 1) DURATION: ROUTE: SPECIES: 19-WEEK INHALATION RAT 1,1,1-T subchronic neurotoxicity APPENDIX R: NEUROPATHOLOGY, MALE The Dow Chemical Company Study ID: K-001716-091 Volume 3 of 3, Page: 63 ANIMAL NUMBER: 90A3798 SEX: MALE DOSE: 0 PPM TEST MATERIAL: MODE OF DEATH: NECROPSY DATE: 1, 1, 1-T SACRIFICED 05/29/90 (DAY 1) DURATION: ROUTE: SPECIES: 19-WEEK INHALATION RAT 60XXC0 cn r* HISTOPATHOLOGIC OBSERVATIONS: BRAIN - MIDBRAIN INFLAMMATION - CHRONIC, MENINGES, UNILATERAL, FOCAL COMMENT: THE INFLAMMATION IS SECONDARY TO PLACEMENT OF AN ELECTRODE TOO DEEP IN THE MENINGES. THE FOLLOWING TISSUES WERE WITHIN NORMAL LIMITS FOR HISTOPATHOLOGY (EXCEPT FOR CHANGES RELATED TO ANY ABOVE OBSERVATIONS): BRAIN - CEREBELLUM, BRAIN - CEREBRUM, BRAIN - FOREBRAIN, BRAIN - MEDULLA OBLONGATA, BRAIN - PONS, DORSAL ROOT GANGLIA, EYES, GASSERIAN GANGLIA, PERIPHERAL NERVE - OPTIC, PERIPHERAL NERVE - SCIATIC, PERIPHERAL NERVE - SURAL, PERIPHERAL NERVE - TIBIAL, SKELETAL MUSCLE, SPINAL CORD - CERVICAL, SPINAL CORD - LUMBAR, SPINAL NERVE ROOTS I END OF REPORT FOR ANIMAL 90A3198 I SL 031110 1,1,1-T subchronic neurotoxicity ANIMAL NUMBER; 90A3 7 99 SEX: MALE DOSE: 0 ANIMAL NUMBER; 90A3800 SEX: MALE DOSE: 0 APPENDIX R: NEUROPATHOLOGY, MALE The Dow Chemical Company Study ID: K-001716-091 Volume 3 of 3, Page: 64 PPM TEST MATERIAL: MODE OF DEATH: NECROPSY DATE: 1,1,1-T SACRIFICED 05/29/90 (DAY 1 END OF REPORT FOR ANIMAL 90A3799 I 1) DURATION: ROUTE: SPECIES: 19-HEEK INHALATION RAT PPM TEST MATERIAL: MODE OF DEATH: NECROPSY DATE: 1,1,1-T SACRIFICED 05/29/90 (DAY I END OF REPORT FOR ANIMAL 90A3800 I 1) DURATION: ROUTE: SPECIES: 19-WEEK INHALATION RAT 1,1,1-T subchronic neurotoxicity APPENDIX R: NEUROPATHOLOGY, MALE The Dow Chemical Company Study ID: K-001716-091 Volume 3 of 3, Page: 65 ANIMAL NUMBER: 90A3804 SEX: MALE DOSE: 0 PPM TEST MATERIAL: MODE OF DEATH: NECROPSY DATE: 1, 1,1-T SACRIFICED 05/29/90 (DAY 1) DURATION: ROUTE: SPECIES: 19-WEEK INHALATION RAT HISTOPATHOLOGIC OBSERVATIONS: U1 -----------------------------------------------------------f BRAIN - MIDBRAIN INFLAMMATION - CHRONIC, HENINGES, UNILATERAL, FOCAL COMMENT: THE INFLAMMATION IS SECONDARY TO PLACEMENT OF AN ELECTRODE TOO DEEP IN THE MENINGES. xxxxeo THE FOLLOWING TISSUES HERE WITHIN NORMAL LIMITS FOR HISTOPATHOLOGY (EXCEPT FOR CHANGES RELATED TO ANY ABOVE OBSERVATIONS): BRAIN - CEREBELLUM, BRAIN - CEREBRUM, BRAIN - FOREBRAIN, BRAIN - MEDULLA OBLONGATA, BRAIN - PONS, DORSAL ROOT GANGLIA, EYES, GASSERIAN GANGLIA, PERIPHERAL NERVE - OPTIC, PERIPHERAL NERVE - SCIATIC, PERIPHERAL NERVE - SURAL, PERIPHERAL NERVE - TIBIAL, SKELETAL MUSCLE, SPINAL CORD - CERVICAL, SPINAL CORD - LUMBAR, SPINAL NERVE ROOTS t END OF REPORT FOR ANIMAL 90A3B04 t 1,1,1 -T subchronic neurotoxicity APPENDIX R: NEUROPATHOLOGY, MALE The Dow Chemical Company Study ID: K-001716-091 Volume 3 of 3, Page: 66 ANIMAL NUMBER: 90A380S SEX: MALE DOSE: 0 PPM TEST MATERIAL: MODE OE DEATH: NECROPSY DATE: I, 1,1-T SACRIFICED 05/29/90 {DAY 1) DURATION: ROUTE: SPECIES: 19-WEEK INHALATION RAT HISTOPATHOLOGIC OBSERVATIONS: BRAIN - FOREBRAIN INFLAMMATION - CHRONIC, MENINGES, UNILATERAL, FOCAL COMMENT: THE INFLAMMATION IS SECONDARY TO PLACEMENT OF AN ELECTRODE TOO DEEP IN THE MENINGES. BRAIN - MIDBRAIN INFLAMMATION - CHRONIC, MENINGES, UNILATERAL, FOCAL COMMENT: THE INFLAMMATION IS SECONDARY TO PLACEMENT OF AN ELECTRODE TOO DEEP IN THE MENINGES. THE FOLLOWING TISSUES HERE WITHIN NORMAL LIMITS FOR HISTOPATHOLOGY (EXCEPT FOR CHANGES RELATED TO ANY ABOVE OBSERVATIONS): BRAIN - CEREBELLUM, BRAIN - CEREBRUM, BRAIN - MEDULLA OBLONGATA, BRAIN - PONS, DORSAL ROOT GANGLIA, EYES, GASSERIAN GANGLIA, PERIPHERAL NERVE - OPTIC, PERIPHERAL NERVE - SCIATIC, PERIPHERAL NERVE - SURAL, PERIPHERAL NERVE - TIBIAL, SKELETAL MUSCLE, SPINAL CORD - CERVICAL, SPINAL CORD - LUMBAR, SPINAL NERVE ROOTS I END OF REPORT FOR ANIMAL 90A3805 I  X X l 1,1,1-T subchronic neurotoxicity ANIMAL NUMBER: 90A3834 SEX: MALE DOSE: 2000 ANIMAL NUMBER: 90A3B35 5EX: MALE DOSE: 2000 APPENDIX R: NEUROPATHOLOGY, HALE The Dow Chemical Company Study ID: K-001716D91 Volume 3 of 3, Page: 67 PPM TEST MATERIAL: MODE OF DEATH: NECROPSY DATE: 1,1,1-T SACRIFICED 05/29/90 (DAY 1 END OF REPORT FOR ANIMAL 90A3834 I 1) DURATION ROUTE SPECIES 19-WEEK INHALATION RAT PPM TEST MATERIAL: MODE OF DEATH: NECROPSY DATE: 1, 1, 1-T SACRIFICED 05/29/90 (DAY I END OE REPORT FOR ANIMAL 90A3835 I 1) DURATION ROUTE SPECIES 19-WEEK INHALATION RAT 1,1,1-T subchronic neurotoxicity APPENDIX R: NEUROPATHOLOGY, HALE The Dow Chemical Company Study ID: K-001716-091 Volume 3 of 3, Page: 68 ANIMAL NUMBER: 90A3837 SEX: MALE DOSE: 2000 PPM TEST MATERIAL: MODE OF DEATH: NECROPSY DATE: 1,1,1-T SACRIFICED 05/29/90 (DAY It DURATION: ROUTE: SPECIES: 19-WEEK INHALATION RAT SL 031114 HISTOPATHOLOGIC OBSERVATIONS: GENERAL COMMENT THE SPECIAL STAINS OF SILVER AND CRESYL ECHT VIOLET-LFB-PAS WERE APPLIED TO EACH TISSUE HITH THE EXCEPTION OF EYES, OPTIC NERVE (WHICH IS ON THE EYE SECTION! AND SKELETAL MUSCLE FROM EACH ANIMAL IN THIS GROUP. UNLESS OTHERWISE STATED, NO ABNORMALITIES WERE DETECTED IN THESE TISSUES USING THESE STAINS. THE FOLLOWING TISSUES WERE WITHIN NORMAL LIMITS FOR HI STOPATHOLOGY (EXCEPT FOR CHANGES RELATED TO ANY ABOVE OBSERVATIONS): BRAIN - CEREBELLUM, BRAIN - CEREBRUM, BRAIN - FOREBRAIN, BRAIN - MEDULLA OBLONGATA, BRAIN - MIDBRAIN, BRAIN - PONS, DORSAL ROOT GANGLIA, EYES, GASSERIAN GANGLIA, PERIPHERAL NERVE - OPTIC, PERIPHERAL NERVE - SCIATIC, PERIPHERAL NERVE - SURAL, PERIPHERAL NERVE - TIBIAL, SKELETAL MUSCLE, SPINAL CORD - CERVICAL, SPINAL CORD - LUMBAR, SPINAL NERVE ROOTS I END OF REPORT FOR ANIMAL 90A3837 I 1,1 J-T subchronic neurotoxicity APPENDIX R: NEUROPATHOLOGY, HALE The Dow Chemical Company Study ID: K-001716-091 Volume 3 of 3, Page: 69 ANIMAL NUMBER: 9 0A 3838 SEX: MALE DOSE: 2000 PPM HISTOPATHOLOGIC OBSERVATIONS: TEST MATERIAL: MODE OF DEATH: NECROPSY DATE: 1,1.1-T SACRIFICED 05/29/90 (DAY 1) DURATION: ROUTE: SPECIES: 19-WEEK INHALATION RAT THE FOLLOWING TISSUES WERE WITHIN NORMAL LIMITS FOR HISTOPATHOLOGY (EXCEPT FOR CHANGES RELATED TO ANY ABOVE OBSERVATIONS]: BRAIN - CEREBELLUM, BRAIN - CEREBRUM, BRAIN - FOREBRAIN, BRAIN - MEDULLA OBLONGATA, BRAIN - MIDBRAIN, BRAIN - PONS, DORSAL ROOT GANGLIA, EYES, GASSERIAN GANGLIA, PERIPHERAL NERVE - OPTIC, PERIPHERAL NERVE - SCIATIC, PERIPHERAL NERVE - SURAL, PERIPHERAL NERVE - TIBIAL, SKELETAL MUSCLE, SPINAL CORD - CERVICAL, SPINAL CORD - LUMBAR, SPINAL NERVE ROOTS t END OF REPORT FOR ANIMAL 90A3838 t ANIMAL NUMBER: 90A3840 SEX: MALE DOSE: 2000 PPM TEST MATERIAL: MODE OF DEATH: NECROPSY DATE: 1,1,1-T SACRIFICED 05/29/90 (DAY I END OF REPORT FOR ANIMAL 90A3840 I 1) DURATION: ROUTE: SPECIES: 19-WEEK INHALATION RAT 1,1,1 -T subchronic neurotoxicity APPENDIX R: NEUROPATHOLOGY, HALE The Dow Chemical Company Study ID: K-001716-091 Volume 3 of 3, Page: 70 ANIMAL NUMBER: 90A3841 SEX: MALE DOSE: 2000 PPM TEST MATERIAL: MODE OF DEATH: NECROPSY DATE: 1, 1, 1-T SACRIFICED 05/29/90 (DAY 1) DURATION: ROUTE: SPECIES: 19-WEEK INHALATION RAT HISTOPATHOLOGIC OBSERVATIONS: BRAIN - MIDBRAIN INFLAMMATION - CHRONIC, MENINGES, UNILATERAL, FOCAL COMMENT: THE CHANGE IS DUE TO INADVERTENT PLACEMENT OF AN ELECTRODE TOO DEEP INTO THE MENINGES. THE FOLLOWING TISSUES WERE WITHIN NORMAL LIMITS FOR HISTOPATHOLOGY (EXCEPT FOR CHANGES RELATED TO ANY ABOVE OBSERVATIONS!: BRAIN - CEREBELLUM, BRAIN - CEREBRUM, BRAIN - FOREBRAIN, BRAIN - MEDULLA OBLONGATA, BRAIN - PONS, DORSAL ROOT GANGLIA, EYES, GASSERIAN GANGLIA, PERIPHERAL NERVE - OPTIC, PERIPHERAL NERVE - SCIATIC, PERIPHERAL NERVE - SURAL, PERIPHERAL NERVE - TIBIAL, SKELETAL MUSCLE, SPINAL CORD - CERVICAL, SPINAL CORD - LUMBAR, SPINAL NERVE ROOTS t END OF REPORT FOR ANIMAL 90A3841 I ANIMAL NUMBER: 90A3842 SEX: MALE DOSE: 2000 PPM TEST MATERIAL: MODE OF DEATH: NECROPSY DATE: 1.1. 1-T SACRIFICED 05/29/90 (DAY I END OF REPORT FOR ANIMAL 90A3S42 I 1) DURATION: ROUTE: SPECIES: 19-WEEK INHALATION RAT U,l-T subchronic neurotoxicity APPENDIX R: NEUROPATHOLOGY, MALE The Dow Chemical Company Study ID: K-001716-091 Volume 3 of 3, Page: 71 ANIMAL NUMBER: 90A3S43 SEX: MALE DOSE: 2000 PPM TEST MATERIAL: MODE OF DEATH: NECROPSY OATE: 1, 1, 1-T SACRIFICED 05/29/90 (DAY I END OF REPORT FOR ANIMAL 90A3843 1 1) DURATION ROUTE SPECIES 19-WEEK INHALATION RAT tX X X t ANIMAL NUMBER: 9QA3B44 SEX: MALE DOSE: 2000 PPM HISTOPATHOLOGIC OBSERVATIONS: TEST MATERIAL: MODE OF DEATH: NECROPSY DATE: 1, 1, 1-T SACRIFICED 05/29/90 (DAY 1) DURATION ROUTE SPECIES 19-WEEK INHALATION RAT THE FOLLOWING TISSUES WERE WITHIN NORMAL LIMITS FOR HISTOPATHOLOGY (EXCEPT FOR CHANGES RELATED TO ANY ABOVE OBSERVATIONS): BRAIN - CEREBELLUM, BRAIN - CEREBRUM, BRAIN - FOREBRAIN, BRAIN - MEDULLA OBLONGATA, BRAIN - MIDBRAIN, BRAIN - PONS, DORSAL ROOT GANGLIA, EYES, GASSERIAN GANGLIA, PERIPHERAL NERVE - OPTIC, PERIPHERAL NERVE - SCIATIC, PERIPHERAL NERVE - SURAL, PERIPHERAL NERVE - TIBIAL, SKELETAL MUSCLE, SPINAL CORD - CERVICAL, SPINAL CORD - LUMBAR, SPINAL NERVE ROOTS t END OF REPORT FOR ANIMAL 90A3844 t, t 1,1,1-T subchronic neurotoxicity APPENDIX R: NEUROPATHOLOGY, HALE The Dow Chemical Company Study ID: K-001716-091 Volume 3 of 3, Page: 72 ANIMAL NUMBER: 90A3B45 SEX: MALE DOSE: 2000 PPM TEST MATERIAL: MODE OF DEATH: NECROPSY DATE: 1,1,1-T SACRIFICED 05/29/90 (DAY 1) DURATION: ROUTE: SPECIES: 19-WEEK INHALATION RAT HISTOPATHOLOGIC OBSERVATIONS: BRAIN - MIDBRAIN INFLAMMATION - CHRONIC, MENINGES, UNILATERAL, FOCAL COMMENT: THE INFLAMMATION IS DUE TO PLACEMENT OF AN ELECTRODE TOO DEEP IN THE MENINGES. THE FOLLOWING TISSUES WERE WITHIN NORMAL LIMITS FOR HISTOPATHOLOCY (EXCEPT FOR CHANGES RELATED TO ANY ABOVE OBSERVATIONS): BRAIN - CEREBELLUM, BRAIN - CEREBRUM, BRAIN - FOREBRAIN, BRAIN - MEDULLA OBLONGATA, BRAIN - PONS, DORSAL ROOT GANGLIA, EYES, GASSERIAN GANGLIA, PERIPHERAL NERVE - OPTIC, PERIPHERAL NERVE - SCIATIC, PERIPHERAL NERVE - SURAL, PERIPHERAL NERVE - TIBIAL, SKELETAL MUSCLE, SPINAL CORD - CERVICAL, SPINAL CORD - LUMBAR, SPINAL NERVE ROOTS I END OF REPORT FOR ANIMAL 90A3B4S t 1,1,1-T subchronic neurotoxicity APPENDIX R; NEUROPATHOLOGY, FEMALE The Dow Chemical Company Study ID: K-001716-091 Volume 3 of 3, Page: 73 ANIMAL NUMBER: 90A3948 SEX: FEMALE DOSE: 0 PPM TEST MATERIAL : MODE OF DEATH : NECROPSY DATE : 1,1,1-T SACRIFICED 05/29/90 (DAY 1 END OF REPORT FOR ANIMAL '90A384 8 1 1) DURATION: ROUTE: SPECIES: 1 9-WEEK INHALATION RAT ANIMAL NUMBER: 90A384 9 SEX: FEMALE DOSE: 0 PPM TEST MATERIAL : MODE OF DEATH ; NECROPSY DATE : 1,1,1-T SACRIFICED 05/29/90 (DAY 1 END OF REPORT FOR ANIMAL 90A3849 1 1) DURATION: ROUTE: SPECIES: 19-WEEK INHALATION RAT fiTrrco ANIMAL NUMBER; 90A3850 SEX: FEMALE DOSE: 0 PPM TEST MATERIAL : MODE OF DEATH ; NECROPSY DATE : 1,1,1-T SACRIFICED 05/29/90 (DAY I END OF REPORT FOR ANIMAL 9QA3850 I 1) DURATION: ROUTE: SPECIES: 19-WEEK INHALATION RAT 1,1,IT subchronic neurotoxicity APPENDIX R: NEUROPATHOLOGY, FEMALE The Dow Chemical Company Study ID: K-001716-091 Volume 3 of 3, Page: 74 ANIMAL NUMBER: 9OA3052 SEX: FEMALE DOSE: 0 PPM TEST MATERIAL: MODE OF DEATH: NECROPSY DATE: 1,1,1-T SACRIFICED 05/29/90 (DAY 1) DURATION: ROUTE: SPECIES: 19-WEEK INHALATION RAT o ^ t0 HISTOPATHOLOGIC OBSERVATIONS: BRAIN - MIDBRAIN INFLAMMATION - CHRONIC, MENINGES, UNILATERAL, FOCAL COMMENT: THE INFLAMMATION IS SECONDARY TO PLACEMENT OF AN ELECTRODE TOO DEEP IN THE MENINGES. GENERAL COMMENT THE SPECIAL STAINS OF SILVER AND CRESYL ECHT VIOLET-LFB-PAS HERE APPLIED TO EACH TISSUE WITH THE EXCEPTION OF EYES, OPTIC NERVE (WHICH IS ON THE EYE SECTION! AND SKELETAL MUSCLE FROM EACH ANIMAL IN THIS GROUP. UNLESS OTHERWISE STATED, NO ABNORMALITIES WERE DETECTED IN THESE TISSUES USING THESE STAINS. THE FOLLOWING TISSUES WERE WITHIN NORMAL LIMITS FOR HISTOPATHOLOGY (EXCEPT FOR CHANGES RELATED TO ANY ABOVE OBSERVATIONS): BRAIN - CEREBELLUM, BRAIN - CEREBRUM, BRAIN - FOREBRAIN, BRAIN - MEDULLA OBLONGATA, BRAIN - PONS, DORSAL ROOT GANGLIA, EYES, GASSERIAN GANGLIA, PERIPHERAL NERVE - OPTIC, PERIPHERAL NERVE - SCIATIC, PERIPHERAL NERVE - SURAL, PERIPHERAL NERVE - TIBIAL, SKELETAL MUSCLE, SPINAL CORD - CERVICAL, SPINAL CORD - LUMBAR, SPINAL NERVE ROOTS I END OF REPORT FOR ANIMAL 90A3852 t 1,1,1-T subchronic neurotoxicity APPENDIX R: NEUROPATHOLOGY, FEMALE The Dow Chemical Company Study ID: K-001716-091 Volume 3 of 3, Page: 75 ANIMAL NUMBER: 90A3BS4 SEX: FEMALE DOSE: 0 PPM TEST MATERIAL: MODE OF DEATH: NECROPSY DATE: 1, I,1-T SACRIFICED 05/29/90 (DAY 1) DURATION: ROUTE: SPECIES: 19-WEEK INHALATION RAT HISTOPATHOLOGIC OBSERVATIONS: SL 031121 THE FOLLOWING TISSUES WERE WITHIN NORMAL LIMITS FOR HISTOPATHOLOGY (EXCEPT FOR CHANGES RELATED TO ANY ABOVE OBSERVATIONS): BRAIN - CEREBELLUM, BRAIN - CEREBRUM, BRAIN - FOREBRAIN, BRAIN - MEDULLA OBLONGATA, BRAIN - MIDBRAIN, BRAIN - PONS, DORSAL ROOT GANGLIA, EYES, GASSERIAN GANGLIA, PERIPHERAL NERVE - OPTIC, PERIPHERAL NERVE - SCIATIC, PERIPHERAL NERVE - SURAL, PERIPHERAL NERVE - T1BIAL, SKELETAL MUSCLE, SPINAL CORD - CERVICAL, SPINAL CORD - LUMBAR, SPINAL NERVE ROOTS I END OF REPORT FOR ANIMAL 9OA3054 I 1,1,1-T subchronic neurotoxicity APPENDIX R: NEUROPATHOLOGY, PEMALE The Dow Chemical Company Study ID: K-001716-091 Volume 3 of 3, Page: 76 ANIMAL NUMBER: 90A3855 SEX: FEMALE DOSE: 0 PPM TEST MATERIAL: MODE OF DEATH: NECROPSY DATE: 1,1,1-T SACRIFICED 05/29/90 (DAY 1) DURATION: ROUTE: SPECIES: 19-WEEK INHALATION RAT SL 031122 HISTOPATHOLOGIC OBSERVATIONS: BRAIN - MIDBRAIN INFLAMMATION - CHRONIC, MENINGES, UNILATERAL, FOCAL COMMENT: THE INFLAMMATION IS SECONDARY TO PLACEMENT OF AN ELECTRODE TOO DEEP IN THE MENINGES. THE FOLLOWING TISSUES WERE WITHIN NORMAL LIMITS FOR HISTOPATHOLOGY (EXCEPT FOR CHANGES RELATED TO ANY ABOVE OBSERVATIONS): BRAIN - CEREBELLUM, BRAIN - CEREBRUM, BRAIN - FOREBRAIN, BRAIN - MEDULLA OBLONGATA, BRAIN - PONS, DORSAL ROOT GANGLIA, EYES, GASSERIAN GANGLIA, PERIPHERAL NERVE - OPTIC, PERIPHERAL NERVE - SCIATIC, PERIPHERAL NERVE - SURAL, PERIPHERAL NERVE - TIBIAL, SKELETAL MUSCLE, SPINAL CORD - CERVICAL, SPINAL CORD - LUMBAR, SPINAL NERVE ROOTS I END OF REPORT FOR ANIMAL 90A3855 I 1,1,1-T subchronic neurotoxicity APPENDIX R: NEUROPATHOLOGY, FEMALE The Dow Chemical Company Study ID: K-001716-091 Volume 3 of 3, Page: 77 ANIMAL NUMBER: 90A3856 SEX: FEMALE DOSE: 0 PPM TEST MATERIAL: MODE OF DEATH: NECROPSY DATE: 1,1,1-T SACRIFICED 05/29/90 (DAY 1) HISTOPATHOLOGIC OBSERVATIONS: BRAIN - FOREBRAIN ATROPHY, GRAY MATTER, UNILATERAL, FOCAL INFLAMMATION - CHRONIC, MENINGES, BILATERAL, MULTIFOCAL COMMENT: THE INFLAMMATION IS SECONDARY TO PLACEMENT OF ELECTRODES TOO DEEP IN THE MENINGES. COMMENT: THE ATROPHIED GRAY MATTER IS LOCATED IN THE CEREBRAL CORTEX JUST BENEATH ONE OF THE INFLAMMATORY MENINGEAL FOCI AND IS SECONDARY TO THE PRESSURE OF AN ELECTRODE PLACED TOO DEEP. BRAIN - MIDBRAIN INFLAMMATION - CHRONIC, MENINGES, BILATERAL, MULTIFOCAL COMMENT: THE INFLAMMATION IS SECONDARY TO PLACEMENT OF ELECTRODES TOO DEEP IN THE MENINGES. THE FOLLOWING TISSUES HERE WITHIN NORMAL LIMITS FOR HISTOPATHOLOGY (EXCEPT FOR CHANGES RELATED TO ANY ABOVE OBSERVATIONS): BRAIN - CEREBELLUM, BRAIN - CEREBRUM, BRAIN - MEDULLA OBLONGATA, BRAIN - PONS, DORSAL ROOT GANGLIA, EYES, GASSERIAN GANGLIA, PERIPHERAL NERVE - OPTIC, PERIPHERAL NERVE - SCIATIC, PERIPHERAL NERVE - SURAL, PERIPHERAL NERVE - TIBIAL, SKELETAL MUSCLE, SPINAL CORD - CERVICAL, SPINAL CORD - LUMBAR, SPINAL NERVE ROOTS t END OF REPORT FOR ANIMAL 90A3856 t DURATION: ROUTE: SPECIES: 19-WEEK INHALATION RAT 1,1,1-T subchronic neurotoxicity APPENDIX R: NEUROPATHOLOGY, FEMALE The Dow Chemical Company Study ID: K-001716-091 Volume 3 of 3, Page: 78 ANIMAL NUMBER: 90A3857 SEX: FEMALE DOSE: 0 PPM TEST MATERIAL: MODE OF DEATH: NECROPSY DATE: 1,1,1-T SACRIFICED 05/29/90 (DAY 1) DURATION: ROUTE: SPECIES: 19-WEEK INHALATION RAT HISTOPATHOLOGIC OBSERVATIONS: f THE FOLLOWING TISSUES HERE WITHIN NORMAL LIMITS FOR HISTOPATHOLOGY (EXCEPT FOR CHANGES RELATED TO ANY ABOVE OBSERVATIONS): BRAIN - CEREBELLUM, BRAIN - CEREBRUM, BRAIN - FOREBRAIN, BRAIN - MEDULLA OBLONGATA, BRAIN - MIDBRAIN, BRAIN - PONS, DORSAL ROOT GANGLIA, EYES, GASSERIAN GANGLIA, PERIPHERAL NERVE - OPTIC, PERIPHERAL NERVE - SCIATIC, PERIPHERAL NERVE - SURAL, PERIPHERAL NERVE - TIBIAL, SKELETAL MUSCLE, SPINAL CORD - CERVICAL, SPINAL CORD - LUMBAR, SPINAL NERVE ROOTS I END OF REPORT FOR ANIMAL 90A385I I ANIMAL NUMBER: 90A3860 SEX: FEMALE DOSE: 0 PPM TEST MATERIAL: MODE OF DEATH; NECROPSY DATE: 1,1,1-T SACRIFICED 05/29/90 (DAY I END OF REPORT FOR ANIMAL 90A3860 I 1) DURATION: ROUTE: SPECIES: 19-WEEK INHALATION RAT ANIMAL NUMBER: 90A3861 SEX: FEMALE DOSE: 0 PPM TEST MATERIAL: MODE OF DEATH: NECROPSY DATE: 1,1,1-T SACRIFICED 05/29/90 (DAY I END OF REPORT FOR ANIMAL 90A3861 I 1) DURATION: ROUTE: SPECIES: 19-WEEK INHALATION RAT 1,1,1-T subchronic neurotoxicity APPENDIX R; NEUROPATHOLOGY, FEMALE The Dow Chemical Company Study ID: K-001716-091 Volume 3 of 3, Page: 79 ANIMAL NUMBER; 90A3890 SEX: FEMALE DOSE: 2000 PPM TEST MATERIAL MODE OF DEATH NECROPSY DATE 1,1,1-T SACRIFICED 05/29/90 (DAY 1 END OF REPORT FOR ANIMAL 90A3890 I 1) DURATION: ROUTE: SPECIES: 19-HEEK INHALATION RAT ANIMAL NUMBER: 9 DA 3 8 91 SEX: FEMALE DOSE; 2000 PPM TEST MATERIAL MODE OF DEATH NECROPSY DATE 1,1, 1-T SACRIFICED 05/29/90 (DAY 1) DURATION: ROUTE: SPECIES: 1 9-WEEK INHALATION RAT HISTOPATHOLOGIC OBSERVATIONS; GENERAL COMMENT THE SPECIAL STAINS OF SILVER AND CRESYL ECHT VIOLET-LFB-PAS WERE APPLIED TO EACH TISSUE WITH THE EXCEPTION OF EYES, OPTIC NERVE (WHICH IS ON THE EYE SECTION! AND SKELETAL MUSCLE FROM EACH ANIMAL IN THIS GROUP. UNLESS OTHERWISE STATED, NO ABNORMALITIES WERE DETECTED IN THESE TISSUES USING THESE STAINS. THE FOLLOWING TISSUES WERE WITHIN NORMAL LIMITS FOR HISTOPATHOLOGY (EXCEPT FOR CHANGES RELATED TO ANY ABOVE OBSERVATIONS): BRAIN - CEREBELLUM, BRAIN - CEREBRUM, BRAIN - FOREBRAIN, BRAIN - MEDULLA OBLONGATA, BRAIN - MIDBRAIN, BRAIN - PONS, DORSAL ROOT GANGLIA, EYES, GASSERIAN GANGLIA, PERIPHERAL NERVE - OPTIC, PERIPHERAL NERVE - SCIATIC, PERIPHERAL NERVE - SURAL, PERIPHERAL NERVE - TIBIAL, SKELETAL MUSCLE, SPINAL CORD - CERVICAL, SPINAL CORD - LUMBAR, SPINAL NERVE ROOTS I END OF REPORT FOR ANIMAL 90A389I I 1,1,1-T subchronic neurotoxicity APPENDIX R: NEUROPATHOLOGY, FEMALE The Dow Chemical Company Study ID: K-001716-091 Volume 3 of 3, Page: 80 ANIMAL NUMBER: 90A3892 SEX: FEMALE DOSE: 2000 PPM HISTOPATHOLOGIC OBSERVATIONS: TEST MATERIAL: MODE OF DEATH: NECROPSY DATE: 1,1,1-T SACRIFICED 05/29/90 (DAY 1) DURATION: ROUTE: SPECIES: 19-WEEK INHALATION RAT THE FOLLOWING TISSUES MERE WITHIN NORMAL LIMITS FOR H1STOPATHOLOGY (EXCEPT FOR CHANGES RELATED TO ANT ABOVE OBSERVATIONS): BRAIN - CEREBELLUM, BRAIN - CEREBRUM, BRAIN - FOREBRAIN, BRAIN - MEDULLA OBLONGATA, BRAIN - MIDBRAIN, BRAIN - PONS, DORSAL ROOT GANGLIA, EYES, GASSERIAN GANGLIA, PERIPHERAL NERVE - OPTIC, PERIPHERAL NERVE - SCIATIC, PERIPHERAL NERVE - SURAL, PERIPHERAL NERVE - TIBIAL, SKELETAL MUSCLE, SPINAL CORD - CERVICAL, SPINAL CORO - LUMBAR, SPINAL NERVE ROOTS I END OF REPORT FOR ANIMAL 90A3892 I 9 Z X lt 1,1,1-T subchronic neurotoxicity APPENDIX R: NEUROPATHOLOGY, FEMALE The Dow Chemical Company Study ID: K-001716-091 Volume 3 of 3, Page: 81 ANIMAL NUMBER: 90A3893 SEX: FEMALE DOSE: 2000 PPM TEST MATERIAL: MODE OF DEATH: NECROPSY DATE: 1,1,1-T SACRIFICED 05/29/90 (DAY 1) DURATION: ROUTE: SPECIES: 19-WEEK INHALATION RAT HISTOPATHOLOGIC OBSERVATIONS: THE FOLLOWING TISSUES WERE WITHIN NORMAL LIMITS FOR HISTOPATHOLOGY (EXCEPT FOR CHANGES RELATED TO ANY ABOVE OBSERVATIONS): yj BRAIN - CEREBELLUM, BRAIN - CEREBRUM, BRAIN - FOREBRAIN, f1 BRAIN - MEDULLA OBLONGATA, BRAIN - MIDBRAIN, BRAIN - PONS, DORSAL ROOT GANGLIA, EYES, GASSERIAN GANGLIA, PERIPHERAL NERVE - OPTIC, PERIPHERAL NERVE - SCIATIC, PERIPHERAL NERVE - SURAL, PERIPHERAL NERVE - TIBIAL, SKELETAL MUSCLE, SPINAL CORD - CERVICAL, SPINAL CORD - LUMBAR, SPINAL NERVE ROOTS I END OF REPORT FOR ANIMAL 90A3B93 I ANIMAL NUMBER: 90A3S95 SEX: FEMALE DOSE: 2000 PPM TEST MATERIAL: MODE OF DEATH: NECROPSY DATE: 1, 1,1-T SACRIFICED 05/29/90 (DAY t END OF REPORT FOR ANIMAL 90A3895 t 1) DURATION: ROUTE: SPECIES: 19-WEEK INHALATION RAT 1,1,1-T subchronic neurotoxicity APPENDIX R: NEUROPATHOLOGY, FEMALE The Dow Chemical Company Study ID: K-001716-091 Volume 3 of 3, Page: 82 ANIMAL NUMBER: 90A389T SEXi FEMALE DOSE: 2000 PPM HISTOPATHOLOGIC OBSERVATIONS: TEST MATERIAL: MODE OF DEATH: NECROPSY DATE: 1,X,1-T SACRIFICED 05/29/90 (DAY 11 DURATION: ROUTE: SPECIES: 19-WEEK INHALATION RAT THE FOLLOWING TISSUES WERE WITHIN NORMAL LIMITS FOR HISTOPATHOLOGY (EXCEPT FOR CHANGES RELATED TO ANY ABOVE OBSERVATIONS): BRAIN - CEREBELLUM, BRAIN - CEREBRUM, BRAIN - FOREBRAIN, BRAIN - MEDULLA OBLONGATA, BRAIN - MIDBRAIN, BRAIN - PONS, DORSAL ROOT GANGLIA, EYES, GASSERIAN GANGLIA, PERIPHERAL NERVE - OPTIC, PERIPHERAL NERVE - SCIATIC, PERIPHERAL NERVE - SURAL, PERIPHERAL NERVE - TIBIAL, SKELETAL MUSCLE, SPINAL CORD - CERVICAL, SPINAL CORD - LUMBAR, yi SPINAL NERVE ROOTS f I END OF REPORT FOR ANIMAL 90A3B97 I gZXTEO ANIMAL NUMBER: 90A3898 SEX: FEMALE DOSE: 2 000 PPM TEST MATERIAL: MODE OF DEATH: NECROPSY DATE: 1,1,1-T SACRIFICED 05/29/90 (DAY I END OF REPORT FOR ANIMAL 90A3898 1 1) DURATION: ROUTE: SPECIES: 1 9-WEEK INHALATION RAT ANIMAL NUMBER: 90A3899 SEX: FEMALE DOSE: 2000 PPM TEST MATERIAL; MODE OF DEATH: NECROPSY DATE: 1, 1, 1-T SACRIFICED 05/29/90 (DAY I END OF REPORT FOR ANIMAL 90A3899 t 1) DURATION: ROUTE: SPECIES: 19-WEEK INHALATION RAT 1,1,1 -T subchronic neurotoxicity The Dow Chemical Company Study ID: K-001716-091 Volume 3 of 3, Page: 83 ANIMAL NUMBER: 90A3900 SEX: FEMALE DOSE: 2000 APPENDIX R: NEUROPATHOLOGY, FEMALE PPM TEST MATERIAL: MODE OF DEATH: NECROPSY DATE: 1, 1- 1-T SACRIFICED 05/29/90 (DAY 1 END OF REPORT FOR ANIMAL 90A3900 1 1) DURATION; ROUTE: SPECIES: 19-WEEK INHALATION RAT ANIMAL NUMBER: 90A3901 SEX: FEMALE DOSE: 2000 PPM HISTOPATHOLOGIC OBSERVATIONS; TEST MATERIAL: MODE OF DEATH: NECROPSY DATE; 1, 1, 1-T SACRIFICED 05/29/90 (DAY 1) , DURATION: ROUTE: SPECIES: 19-WEEK INHALATION RAT THE FOLLOWING TISSUES WERE WITHIN NORMAL LIMITS FOR HISTOPATHOLOGY (EXCEPT FOR CHANGES RELATED TO ANY ABOVE OBSERVATIONS): BRAIN - CEREBELLUM, BRAIN - CEREBRUM, BRAIN - FOREBRAIN, BRAIN - MEDULLA OBLONGATA, BRAIN - MIDBRAIN, BRAIN - PONS, DORSAL ROOT GANGLIA, EYES, GASSERIAN GANGLIA, PERIPHERAL NERVE - OPTIC, PERIPHERAL NERVE - SCIATIC, PERIPHERAL NERVE - SURAL, PERIPHERAL NERVE - TIBIAL, SKELETAL MUSCLE, SPINAL CORD - CERVICAL, SPINAL CORD - LUMBAR, SPINAL NERVE ROOTS I END OF REPORT FOR ANIMAL 90A3901 I