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AR2F26-E01FI% ACUTE TOXICITY TO AQUATIC INVERTEBRATES (E.G..DAPHNIA) TEST SUBSTANCE Identity: aPserPflFuOorSooLcitsaanlet,suFlCf-o9na4t,eo,rLFiCt-h9iu4m-Xs.alt(;1m-aOcytaanlessoubleforneifceracriedd,to 1,12,2,3,3,4,4,5,5,6,6,7,7,8,8,8-heptadecafluoro-, CAS #20457-72.5) lithium salt, R(aepmparrokxism:atTehley t2e4s.t5s%amtpesltesiusbastmainxcteuraeonfdt7h5e.5te%stwastuebrs)t.anNcoecianlwcaultaetrions `twheerteesmtasdaemptloea.djustfor the actual concentrationofthe test substance in METHOD Method: Not noted. Test type: Static acute GLP: No Year Completed: 1994 Species: Daphnia magna Analytical Statistical monitoring: pH methods: ECs and DO content values calculated using Trimmed Spearman- Karber method Test daphnid Minnesota. source: Obtained from U.S. EPA-NETAC, Duluth, Test daphnid age at study initiation: <24-hours . Test conditions * Dilution Dilution water: water Carbon-fitered chemistry: well water pH: 84 Do: 8.6 mglL wSatoscpkraenpadrteedsitnsdoilluuttiioonnwsaptree{pr0aryaiteilodna: tAesptrsiammarpylestcoocnkcesonlturtaitoinon aopfp1r0o0pr0imagte/La.moAlulnttesotfssotlouctkiosnosluwteironewmitahddeilbuytidoinlwutaitnegrtthoe make 50 mL of solution per concentration. Exposure solution. vessels: 100 mL glass beakers containing 50 mLoftest Number Number of of replicates: 4 daphnids per replicate: 5 Number of concentrations: Water chemistry during the five plus study: a negative control Dissolved oxygen at test termination: 7.0 mglL (control exposure) 001535 7.8 mglL. (1000 mg/L exposure) pH at t8e.s6t t(ecronmtirnoalteixopno:sure) 8.6 (1000 mg/L exposure) Test te2m0p.e1r2a1t.u0reCrange (0 48 hours) Element basis: mortality and immobilization RESULTS Nominal Element vcaolnucee:ntrat2i4o-nhso:urBEKCcsoont=ro3l3,010(02,001-8307,03)2m0,g/5L60, 1000 mg/L 48-hour ECs = 210 (190-230) mg/L Statistical ~: 48-hour Evaluation: The NOEC EC50 = 100 values mg/L and 95% confidence intervals `were calculated using the Trimmed Spearman-Karber method with trim set 0 0%. MRoermtaarliktsy:of cVoanltureolssr:eNpoornteed are for the test sample. No calculations were made sample. to adjustforthe concentrationofthe test substance in the test CONCLUSIONS _ -- Texhheibtietsetdsaam4p8l-ehocuorntEaCisnoifnogr2D4a.p5h%niPaermfalugonraooocfta2n1e0sumlgflonLa.te, Lithium salt SStu.bmPaiutlt,erM:inne3sMotCao,m5p5a1n3y3, Environmental Laboratory, P.O: Box 33331, . DATA QUALITY aRnealliyatbilclailtyd:atKaiifomri:scdhetrearnmkiinnagti2o.n oTfhitshesttuedsty,swuhbislteanweclelccoonncdeuncttreadt,iolnaicnksthe test solutions; and determinationofthe sample purity. REFERENCES "LTahbiosrsattuodryy,wLaasbcRoenqduuecsttednbuymbteher 3MM10C1o8m,p2a/n1y0/,04E.nvironmental OTHER Last changed: 5/2/00 001536 Lo. wmscncmoEnme--rs--aom0srony1 ameed TYPETEST: + STATIC __K___ STATIC RENEWAL -- TEST SUBSTANCE: smn SOM =X ua "TESTDAPHNIDSOURCE: U.S.EPA-NETAC,Duluth,Mn. DATE RECEIVED: _(0-3B- QO r-- geeest TRE LOADING RATE: oe L__ TEST SOLUTION VOLUME: an oo 2s DATEINTIATED: 2-3-03.8 wng Soml SIZE OFTESTVESSELS: 100mL DATE TERMINATED:_3-10-04 14116 TEST | [pice|v5] E ETE d or aa 'BK1 |CONTROL| ~ [ |T 0 %|6 ao| x2 | contro] seo eT Bxs |conRoL| seo ToTNTN " BK4 |conTROL| - feo ToTTT (1TT 1 R21 oe a0 [0 1 TRG -e| 12 Feo ToT T+ 13 ToToTNTN 14 oo171 ISN A - 21 180 Ww [Lo ATR TE S | 22 CoT&T TF1 23 [CoTaTNT3 24 [ooTT 1 -- --T--1T . a1 I] en [aTFTee[=a | 32 a1 1~s T1T :--1 23 CsTNT 34 a1s171 1TT ar 60 wa [5T518 [68 a| a2 =I T ~s 1T 1 43 [Cs1s TT N11 a [ssT7717 1 TT ta L : aa ` &1 woo [a0 |S |5 86[x | 82 CsTST 1T1- 53 Cs1&8 TST51 4 ssT7171 1 TT 'ECS0(95%C.LIMYL Ak Lap SO meu Q-3I0D Uestees(ne Ake `COMMENTS * gS AO L On phen ALLEY A. wis mb Seel. was Wen ANALYST: SUBD Sp 001537 Riphcoes anes combinle @ Xesk Aecomtaaition PR a DLO. Mecsiteenks [ENVIRONMENTALLABORATORY LR Miots Date: 2/8/94 Analyst: JBD Daphnia magna 48-hour Toxicity Test Data Summary SAMPLE: FC-94-X `Sample Conc. |No.Organisms] (mg/L) 100 20 180 20 320 20 560 20 1000 20 %Immobilized @ 24 hours 48 hours 0 0 0 25 45 100 100 100 100 . 100 24'h EC50 (95% Cll.) = 48 h ECS0 (95% Cll.) = 330 mg/L (290-370) 210 mg/L (190-230) [ECS0 Estimation Method: Trimmed Spearman-Karber - 24 h: 0% trim 48 h: 0% trim e\A . 3 Pi=e, N & < NN" +<. ~,> , % IRN | Re , / ES I 001538 co EST TwR5AI5LM0IMENADGN.TDOSNLPEoRRSERSMEVAAANRLKCUAHER.SBETIRnNC.bTiEoTAHSOeDRvFeOR CALCULATION GF TFFoOARRI.NREBEDAF.MEIRSLEPTNEOCANER..HANCR-ICKT.AERDREURSSOM,ETHANODD.RF.OVR. ETSHTUIRMSATTOINN.G H1E97a7r.N CELNEoVTRIHRRAEOLTNI.CoONNSCCEI1N:3T0RATA)ETCIH1ONN7OSL.. I(N113170T78O))X.IS7I1T4Y-71B9IOASSAYS. : CreDaarneweAL FR2i/eo8tt/es94 SEST COPY A: VAILABLE RDSaUrReApcTiaIeOrsNa Tro5urreesams . NNCOUGNMRCBTEEaNRLTIRTSAIxTErISoOsNE(DhG/L) 100.000S 189.0U0I"c $20.0TU05E $0.0xTe0m 1000.00=0 CSPPEEAARRMMAANN-KKAARREGBEERR95%ETRSITLNIOMWEARTESCONFIDENaCcEso|0.00s27.12552520982 75% Urencon Toeuee EA . 001539 i Mmiol -- AM houss yFST COPY AVAILABLE ------S-- URLINGTO-- NRESEARC-- H.INC. 6.507 Trimmed Soearman-Karber Methad ECS0 and LCSO Values in fBorioaCsaslacvuslatisn of x 3 6.08483 3 3 s.180%3 N zo 3x 2 am7E N 3 c 3 % 3. T S.as3 2 3 a 3 T 3 1 2 N0 2.3908 3 33 1.7266 33 .3 w3e38E x o' x . | : . ni5e 5 "= . = .- 3 . @D LeoDDLeaD DsDDDD DDDDE DDDDD DLDcEsD DoDDDD DDDDD DDDD DDLDeE7I DsDDDL DDDDD DDDDE LDcDiEo0 . MORTALITIES 001540 CrCpheaenoa Srecies hF2i/e8te/a9a55 magna : sCNaUOBuMNUBGREpETRaNItTaERNxApToIoOENs(n/L) --aE1m0n0e.02u0res18. 0.0T0E 520.0TE0D 540.0O0R 1000.00g0 NoSSRRPTEEAnAIRRTrMIaAENNS-KKeAnRsGsEnR ETSRTIINMATES ec act5o|0.x 00207.5736053 2 S2e5i% LGOeWrEeRn. CcOoNnFeIiDbEeRnGcEe fToeises " BEST COPY AVAILABLE 001541