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ACTIVATED SLUDGE RESPIRATION INHIBITION TEST
TEST SUBSTANCE________________________________________________
Identity: A mixture containing perfluorooctanesulfonate, which may also be referred to as PFOS, FC-95, or as a component of FC-206CE. (1Octanesulfonic add) (CAS # 2795-39-3).
Rem arks: The 3M production lot number was 502. The test sample is FC-206CE. Current information indicates it is a mixture of 0.92% PFOS, 15% diethylene glycol butyl ether, 76.09% water, 4% urea, 1.28% Sultone foamer, 1.55% sodium octyl sulfate, 1.05% polyoxyethylene monooctylphenyl ether, 0.06% sodium lauryl sulfate, and 0.05% tolytriazole.
The following summary applies to a mixture with incompletely characterized concentrations o f impurities. Data may not accurately reflect the toxicity o f the fluorochemical component o f the test sample.
M E T H O D _________________________________________________________
Method: OECD 209 Test type: Acute static GLP: No Y ear Com pleted: 1989 A nalytical m onitoring: Dissolved oxygen concentrations, pH, and temp. S tatistical m ethods: Results were determined by calculation of the % inhibition in respiration rate. Test organism source: Activated sludge mixed liquor collected from the Metro W aste Treatment Plant, St. Paul, MN. Test condition:
Dilution w ater: Millipore Milli-QTM water Synthetic sew age: per OECD method Reference and te st solution preparation: A stock solution of the reference substance, 3,5-dichlorophenol, was prepared by dissolving 500 mg in 10mL 1N NaOH, diluted to 30mL with Millipore Milli-QTM water, then brought to the point of incipient predpitation with 1N H2S 0 4 and diluted to 1L with Milli-QTM water. The pH of the reference solution was measured to be 7.6. Test substance was added directly to the test vessels and was apparently miscible with water. T est vessels: Not given. Num ber o f concentrations: five plus 1 reference substance and 2 blank controls Tem perature: 2 0 - 2 2 C Total suspended so lids and pH on day o f testing: 3.6 g/L (TSS), initial pH 7.3 and final pH 7.3 Elem ent Basis: Respiration inhibition as determined by oxygen consumption.
001755
Rem arks: Values corrected to 20 C for calculations. RESULTS________________________________________________________ Nom inal concentrations: 2 Blank controls, reference substance, 100,180, 320, 560, and 1000 mg/L Elem ent values: 30-minute EC50 = >1000 mg/L
3-hour EC50 = >1000 mg/L Element values based on nominal concentrations. Rem arks: Testing was conducted on a mixture as described in the Test Substance Remarks field. The values reported apply to that mixture and not the fluorochemical proportion alone. C O N C L U S IO N S ___________________________________________________ The FC-206CE 3-hour EC50 for activated sludge respiration inhibition was determined to be >1000 mg/L. Subm itter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY___________________________________________________ R eliability: Klimisch ranking 2. Testing meets all criteria for quality testing. However, sample purity was not properly characterized and the study lacks analytical confirmation of the amount of fluorochemical proportion in the solution. R E F E R E N C E S ____________________________________________________ This study was conducted by the 3M Company, Environmental Laboratory, St Paul, MN, Lab Request number G1358-2,1989. O T H E R __________________________________________________________ Last changed: 6/26/00
001756
Type Test o
ENVIRONMENTAL LABORATORY ACTIVATED SLUDGE MIXED LIQUOR
ul G / 3 S S ^ Data (3 - 2 - 3 * 1
Analyst
A e r a t e d . S lu J^ g .
f a i \ o k X n \ n t 'h lH -J M T fS /~ itzc ti
source/4e^-f/o U J b ^ f e ~ f~ r e A f r h e ^
S~/~ F ^ u l j
___________
Date O btained b - 3 - & <?____________ _ Date Used 6
3 ~ . ____________
Mixed L iq u o r Suspended S o lid s (MLSS) 3 . in g/L Adjustm ent A/rm <p g/L
p u p ni+idJ. 7 3 ____ ^VhduU 7 . 3
Comments T e S J- P tm U M l L .ft C r i y ^ O __________ =._________________________
Test Substance Sample D e s c rip t io n F CL - &>Q(o
Sample pHM^beiA-Cove^ /oo on ^U -
Comments ___ ____
L
Adjustment )^QY\
________
Reference Substance
Sample D e s c rip tio n 3 . A - t > /c K Infophenol_____
Sample pH Z lo SiccV. <.olh.\__ Adjustment A/Qftt
Comments
___________
Exposure Conditions Test Duration ,3 -/ f c > u ir Teat Temperature 2 0 * 2 3 . C Comments.___________________________
001757
Form 10620-6-D-PWO
Sample Description -C ~ Z O \o i Lo\ $09^ y e lle d
Environmental Lab -- Work Sheet
OECD ACTIVATED SLUDGE RESPIRATION IhHIBITION TEST #209
Test Solutions Preparatimi
Lab Request No. 6 I3 5 ~ . Paoe__ I_ _ o f _ _ 3 ._ _ Date: ___ - 2 3 ~ S <\ ____
Analyst
_____________________________ Atia.
Test Cone, mg added ml Df"water ml syn. sewage ml microbial
Total volume
fp*H npr SMI ml Innrnl.- rr 5IW ml
ml
dU\Vc. ;(0 C e n tro ^
J L lt>
t- J O O ________ ' ____S ~ O Q
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p r^ p a rg ^ t L - l < \ - Z < \ t q g ro ^ lo w <^ r k > * L U - * o - 8 3. ___
ENVIRONMENTAL LABORATORY
lr Lz.l&53-L
OECD ACTIVATED SLUDGE RESPIRATION INHIBITION TEST #209
RAW DATA
Test Substance:.
- .oL-i C & ___ l o t TOaL
Source o f Activated Sludge Mixed Liquor: M e + ro tlJ a< ;+g . Tfg.aiwnejnH
Date Received: 6 - Z 3 - S 9
Date Used:
________
Analyst:
BEST COPY AVAILABLE
30 - MINUTE EXPOSURE
DISSOLVED OXYGEN CONCEIT
lest substance
"TTRP MINUTE
1
Concentration (mg/L) 0.5 1.0 2.0 3.0 4.0 5.0 6.0 7.0 8.0 9.0 10.0
CONTROL I
in n 190 3n /y h n /O D O
7 - i 7 - 3 !o L . 1 5 . Ip s . \ H .S ' V . D 3 S 3 . 0
fl.\ 7H 7 .3 b #
5 .3 5 .3
H .3 3 .9 ^3 e
7 i f ? 7 . 5 7 * o a?*1! 5 . 9
H .S
3 . 7 3.3. 9 . 7 .
7-1 7 1
L l & b 5*0
3 .9 3 . ^
*0 0
7 A (o s .g 5 . 1 V -5" 3 . 9 3 - 1
1 .8 / J L .
q .b 3 A 3 1
O.S 1-7
?/
CONTROL I I
3 - HOUR EXPOSURE
lest substance Concentration (mg/L) CONTROL I
lo o Id o 3 9 .0 S /o O /o o a
Z L ip, jL L 5 5 . 0
! . 3.
J Ji ' 4 346
DISSOLVED OXYGEN CONCENTRATIONS mo/L
riME-MINUTES 0.5 1. 0 2. 0 3.0 4.0 5.0 6.0 7.0 8.0 9.0 10.0
7 ,b 7 .P - .7
5 * k I H .b Y.|
3 .0 A >5
$ .0 7 .< \ 7 . 5 7-1 0 ,7
3 " - $ 5-1 d Q D
YJ_
9> .?
7.7 7 . 4 7 ,0 L ,b bi. 5 ? 5 . S *1 Y - 7
5 73 . 1 7 . 7 7 . 3 lpf\ la. 5 >.|
y. 5
$ . 1 7 . B 7.5" 7.1
Y-10 . 4 u>o ,5 .7 5 , 3
s . 7 - 8 7.iT 7.1 u n 6.3 5 - .1 5 . 5 5 : 1 Y-7 5 5
CONTROL I I
Z i. 7/
pH o f Activated Sludge.Mixed Liquor:
pH o f highest concentration tested:
Test Temperature:
c
^
, 0 5 . f T 5 / 0 Y - v 3 . 1 3 . 3 3 5 ?.*>
In itia l 7 .3 In itia l
j
Final 7 3
Final y r-- ` ^
001759
ENVIRONMENTAL LABORATORY
ACTIVATED SLUDGE RESPIRATION INHIBITION TEST #209 CALCULATION OF RESULTS
Test Snhctanrg ^(L " ^ Ota (Li- L-P+
30 - MINUTE EXPOSURE
Test Substance Concentration (mg/L)
Oxygen Uptake Rate R
IL I __ .________ 1 joo O5000
IdO
-zao S~>o
0 . S3SS 0 . b>oot>
0 . bh/A
/ooo
O. 7
R Corrected^ to 20 C o.H'-i O-Hub .SSB 0-S73I oltiZLi O.liAl
LR & 1 3 5 2 'I Date Analyst
p& z i? 2 >
% Inhibition in Respiration Ratel*J -- --
4kmm
--
CONTROL II
0 ..A/A1 _____ 0 .Hip77_____
--
%Percent Difference in Cont:rols^ A ci> ^ 0
EC50 (95% Confidence Limit:si mon.
> loco ma^lL______________________ es
3 - HOUR FYPOSIIRF.
Test Substance Concentration (mg/L)
Oxygen Uptake Rate R
SISLJ___________ 0 .525S~
loO
O.i/H0 0
.... - ISO
0 3 77b
6 . H0 6 0
. . . O. S51L
! OOO
O
R Corrected^ to 2(fC
o.HidH o.HZoo o. 3 7 7 4 . 0 . Hooo O. IS 7b o.-zms'
% Inhibition in ,2i Respiration Ratel^J
--
13_____ ZL ' 17 b I . .................
CONTROL II
0 . SM 1_______
Percent Difference in Controls^ O 7
EC^ (95% Confidence Limits) mg/L,.Q O i?
CHZ2 1 ____
(1) Temp. Corrections
(mg 02/L-min.)
&C, + RC^\
( 2 7 - Rg
(2) Inhibition!*----- x 100
lI W
Where: R Oxygen uptake rate (mg 02/L-min.) T,obs Sample temp., C* & - 1.047, x - T,obs - 20*C C Control
(3) % Difference -- ----**-Xl0 fRC-, + RC\
r^ 7
The two control respiration rates should be within 15% of each othe
See. pa i.ociesJ~ o\n.
pre?
001760
Forni 10*20-O - P W O
613S&-R.
Lab Request No. ^ J7>&
Sample Description
\ 5- lo ro P h en o \ deference.5JbS4anee
lLftrtd<~ 8*SL a.vat> of
Environmental Lab -- Work Sheet
oecd activated sludge
RESPIRATION IhHIBITION TEST #209
Page
Date: Analyst:
purpi# crys.faU_______ Stock and Test Solutions Preparation
I
of 3 3 "^ ?
CTOCKSQUJTIQN; ^ ^ 3.SKcf>/ l
<xcL homi = q S
--------3jS- 'hcP ik jOm A -- /qjuAjxoH-__________________ ___
___________-l> i*lu4> A . fc> 3 d rn& u H rL M \ lir - ? _ u j> a 4 r ______________ ___
----------------- - A A A eJL . u n ie r 6 W r n n ^ j h a J
fn p tm s h u n t i 1 m ip te n f f>p
------------ r i f i u t a i .
lifer LPHrto >M tl(i- & Loads,<.___________
__________ - S i e c t S a im v . p J i r J Z ' k ____________________________________________
Test Cone. ml stock soin ml DI water ml syn. sewage ml microbial ^mg/L__ per 500 ml per 500 ml feed per 500 ml inoculum per 500 ml
Ckrn>f4cr/>,k#0 --
Z2H *
ib +- oo
=
InJ - g - ______ ____
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^>
--
Total volume ml
Sco
Sot>
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7 S L
M ) i r - g c o o le r us**
___ p t i - b t O
_______
.PWftSrf?A (p -W - j ....<Mr d liran- f a .r * f c l
001761
' -< :; [
ENVIRONMENTAL LABORATORY
L R ^ L ilS L -r
OECD ACTIVATED SLUDGE RESPIRATION INHIBITION TEST #209
RAW DATA
Test Substance:__ 3
iV U I o c o p h en n I f e & r e n f e , ^ b ^ - k M c e .___________ _
Source o f Activated Sludge Mixed Liquor: M e+ro UJa^+ertfeoAvnert- P l a n f S+Bi u L m v
Date Recelued:
SH
Date Used: b - J Z - S H _______
Analyst:
2 . P j>Ol/ L
best copy available
30 - MINUTE EXPOSURE
les t buDstance Concentration (mg/LJ
CONTROL I
Id
0.5 1.0 2.0
7*3 7,\ L b
7.H 7 ii bA
TTRFHINUTe
TEM
3.0 4.0 5.0 6.0 7.0 8.0 9.0 10.0 C*
t i S S 5.0 `t s H-o 3.6>
>>b L tf.O 5 .7 5 , i .1 f/?
a
CONTROL I I
2 7^ jLL S n 5 .1 L L 2 2 . i d n
3 - HOUR EXPOSURE
lest Substance Concentration (mg/L)
0.5
1.0
2.0
3.0
TTFTRI nutes
4.0 5.0 6.0 7.0
8.0
9.0
10.0 TEc*!
CONTROL I
7.3 71 6.5"
5~. fo
33
ID
.3
f?.\ 7i1 7.7 I S 7,7 7.c> f,jR
A a
---
CONTROL I I
7-4 7.1 hlo UA 5 . r 5 .0 y . r 2 c . 2 1
pH o f A ctivated Sludge Mixed Liquor;
pH o f highest concentration tested:
Test Temperature:
In 1 t1 a l7 #S In itia l
Final 7 . 5 "
Final "J%<\
id
001762
Test Substance
UNViKUNMliNl'AL LABORATORY
ACTIVATED SLUDGE RESPIRATION INHIBITION TEST #209 CALCULATION OF RESULTS
ic WL rQphonal fle-fe. flute Suk.'km cp
30 - MINUTE EXPOSURE
LRjjg.17SQ
Date Analyst
P&3<9#3
Test Substance Concentration (mg/L)
CONTROL I
1 1~>
Oxygen Uptake Rate R
, ? tn O O
R Corrected to 20*C
O M b ll
, 7 1 7
t Inhibition in Respiration Rate'^
--
H.3,
CONTROL IT
O - S H z V ______
Percent Difference in Controls^ .5* t* 'eC50 (95 Confidence Limits) mg/L iO ^ t il \i\AuCfJL ^3./hiLttm.
Test Substance Concentration (mg/L)
ID
Oxygen Uptake Rate R
0 O .<2
1
R Corrected to 20TC
o
\r
t Inhibition in ,2i Respiration Rate^J -
5 1 ,.....
*
1
CONTROL II
Q`5 9 ,& r
71V
--
Percent Difference in Controls^____ 0 * 7 __________
EC5Q (95% Confidence Limits) mg/L
^ \ j7.7
/P h,1LttlMV.
(l^ T w lc o rre c tia ^
(3) I Differences \.1~-- X 100
f RCi + RCA
(2) I Inhibition .............g X 100
The two control respiration rates should be within 151 of each othe
p ^ A . -Gs>C
4-
Where: R Oxygen uptake rate (mg 02/L-min.)
++ fie in o ' p re p
T,obs Sample team., C* .
_
6 - 1.047, X - T,obs - 20*c
BEST COPY AVAILABLE
C Control
001763
