Document NGrkBkbzbMvnMg50D6OR1bGkb

BIOCONCENTRATION ARWfr035* TEST SUBSTANCE Identity: N-ethylperfluorooctane sulfonamidoethanol; may also be referred to as N-EtFOSE Alcohol or FM-3422. (1-Octanesulfonamide, N-ethylI.I^^.S.S^AS.S.e.ej^.S.S.S-heptadecafluoro-N-^-hydroxyethyl)-, CAS # 1691-99-2) Remarks: Material is an off-white, waxy solid of uncharacterized purity. METHOD:________________________________________________________ Method/guideline followed: 3M derived method outlined in the test procedure for determining the aquatic Bioconcentration Factor of N-EtFOSE alcohol. Type: Static exposure with a flow-through clearance phase. GLP (Y/N): No Year: 1978 Species: Channel catfish (Ictalurus punctatus) Supplier: Not noted in the report. Analytical monitoring: Concentration of N-EtFOSE alcohol in water and fish by GC with electron capture; temperature Length and weight: Not noted in the report. Exposure period: 7 days Clearance period: 5 days Statistical methods: 3M Trac system, MINITAB II program Test fish age: Not noted in report. Loading: Not noted in report. Pretreatment: Not noted. Test conditions: Dilution water: Carbon-filtered well water Dilution water chemistry: Not included in report. Stock and test solution preparation: The primary stock solution was prepared by dissolving 57 grams of N-EtFOSE alcohol in 50 ml of acetone, applying the solution to 10 pounds of 3.5 mm glass beads, evaporating off the acetone, and circulating carbon filtered well water through the glass beads. The system was designed to result in a final loading ratio of 0.5 grams per liter. Aerobic conditions were maintained throughout and the system was assumed to go to saturation. Length of time allowed to circulate was not noted. Exposure vessels: 114 liter aquarium. Volume utilized was not noted. Number of replicates: Two Number of fish per replicate: Not noted in the report. Number of concentrations: One plus negative control Water chemistry during the study: Dissolved oxygen: Not noted in the report. 006242 Test temperature: 21+1C pH: Not noted in the report. Photoperiod: 16 hours light and 8 hours dark with a 30 minute transition period. Remarks field: Following the introduction of the channel catfish into the experimental and control aquaria samples of 4 channel catfish and three water samples, top, middle and bottom layer, for N-EtFOSE alcohol uptake analysis were obtained at the following time periods: Control, prior to introduction, 15 minutes, one hour, two hours, four hours, eight hours, twelve hours, sixteen hours, twenty hours, twenty-four hours, and at twenty-four hour intervals for a period of 7 days. The channel catfish were then removed to clearance tanks for two hours and finally transferred to a flow-through system having flow rates through the test chambers of four water volumes per 24 hours. A sampling regimen identical to that of the uptake phase of the study was immediately initiated and terminated after five days. RESULTS________________________________________________________ N-EtFOSE Alcohol Uptake by Channel Catfish (Ictalarus punctatus) - Whole fish samples Time 15 min. 1 hour 2 hours 4 hours 8 hours 12 hours 16 hours 20 hours 24 hours 48 hours 72 hours 96 hours 120 hours 144 hours 168 hours Fish Group A (ug/g) 1 4 9 16 58 62 51 61 104 132 222 190 211 320 552 Fish Group B (ug/g) 1 4 10 29 42 60 56 74 110 213 159 257 201 279 158 006243 N-EtFOSE Alcohol Clearance of Channel Catfish (Ictalarus punctatus) - Whole fish samples Time 15 min. 1 hour 2 hours 4 hours 8 hours 12 hours 16 hours 20 hours 24 hours 48 hours 72 hours 96 hours 120 hours bSample lost. Fish Group A (ug/g) 306 269 252 260 260 253 235 238 237 b 200 136 106 Fish Group B (ug/g) 319 216 309 242 242 206 177 255 201 190 227 226 78 N-EtFOSE Alcohol Uptake of Channel Catfish (Ictalarus punctatus) Muscle Time Control 15 min. 1 hour 2 hours 4 hours 8 hours 12 hours 16 hours 20 hours 24 hours 48 hours 72 hours 96 hours 120 hours 144 hours 168 hours Muscle Concentration (ug/g) <0.1 <0.1 3 5 10 22 31 38 49 61 118 127 135 153 197 147 006244 N-EtFOSE Alcohol Clearance of Channel Catfish (Ictalarus punctatus) Muscle Time 15 min. 1 hour 2 hours 4 hours 8 hours 12 hours 16 hours 20 hours 24 hours 48 hours 72 hours 96 hours 120 hours Muscle Concentration (ug/g) 118 127 108 128 147 148 181 117 75 102 77 69 72 N-EtFOSE Alcohol Uptake by Brain Tissue and Gastrointestinal Tract of Channel Catfish (Ictalarus punctatus) Time Control 15 min. 1 hour 2 hours 4 hours 8 hours 12 hours 16 hours 20 hours 24 hours 48 hours 72 hours 96 hours 120 hours 144 hours 168 hours Brain Concentration (ug/g) <0.1 2 8 28 50 100 115 149 240 225 352 328 606 588 695 905 Gastrointestinal Tract Concentration (ug/g) <0.1 <0.1 6 12 41 54 110 119 206 201 230 510 533 504 688 533 006245 N-EtFOSE Alcohol Clearance of Brain Tissue and Gastrointestinal Tract of Channel Catfish (Ictalarus punctatus) Time 15 min. 1 hour 2 hours 4 hours 8 hours 12 hours 16 hours 20 hours 24 hours 48 hours 72 hours 96 hours 120 hours Brain Concentration (ug/g) 1152 761 712 602 511 582 618 559 523 586 450 423 417 Gastrointestinal Tract Concentration (ug/g) 281 630 325 507 302 263 1205 805 564 264 353 390 712 N-EtFOSE Alcohol Uptake by Liver, Kidney, and Gall Bladder of Channel Catfish (Ictalarus punctatus) Time 15 min. 1 hour 2 hours 4 hours 8 hours 12 hours 16 hours 20 hours 24 hours 48 hours 72 hours 96 hours 120 hours 144 hours 168 hours LiverConcentration (ug/g) <0.1 11 11 31 67 89 99 161 149 329 261 481 469 384 561 Kidney Concentration (ug/g) <0.1 <0.1 <0.1 37 59 77 93 149 99 189 200 359 315 349 152 Gall Bladder Concentration (ug/g) <0.1 <0.1 <0.1 <0.1 <0.1 158 245 271 242 1383 1585 1573 2875 3261 13805 006246 N-EtFOSE Alcohol Clearance of Liver, Kidney, and Gall Bladder of Channel Catfish (Ictalarus punctatus) Time 15 min. 1 hour 2 hours 4 hours 8 hours 12 hours 16 hours 20 hours 24 hours 48 hours 72 hours 96 hours 120 hours LiverConcentration (ug/g) 504 650 758 779 507 446 728 420 443 575 316 76 507 Kidney Concentration (ug/g) 274 567 439 447 390 559 515 365 259 351 203 182 285 Gall Bladder Concentration (ug/g) 1543 4908 1286 5058 3710 4321 4247 2495 3912 2810 1221 1411 6497 CONCLUSIONS No reliable conclusions can be derived from this study. General observations can be made. N-EtFOSE alcohol concentrations increased in the fish tissues over the course of exposure. Lipophilic organs and organs possessing a large surface area at the water/organ interface possess the highest uptake rate constants. The brain and gastrointestinal tract have potentially high bioconcentration factors due to the lipophilic nature of N-EtFOSE alcohol. The muscle fillet appeared to exhibit the least potential to bioaccumulate NEtFOSE alcohol. The gall bladder showed the highest concentration of N-EtFOSE alcohol There is a potential for excretion of N-EtFOSE alcohol through both urinary and fecal pathways. No signs of toxicity were apparent at the exposure concentration. While concentration decreased in the whole fish samples at the end of the clearance phase, the time required for complete removal is longer than the 5 days used in the test. 006247 Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY____________________________________________________ Reliability: Klimisch ranking 3. There is a lack of description for the study's methodology and characterization of the test organisms. There is no attempt at correlating, nor is there any record of, the size, age, and body fat content of the fish as it relates to the concentration of N-EtFOSE alcohol found in the various samples. This is especially important due to the fact that older, larger fish are likely to have more fat content than younger, smaller ones. The vigorous method used to solubilize the test substance appears to have resulted in a supersaturated solution. The solubility is characterized as being 50 ppb, but the concentration in the aquarium was given to be 500 ppb, and it was noted that the N-EtFOSE alcohol was present as a suspension. Moreover, it was also noted that excitability of the test organisms would cause disturbance of the bottom layer and re-suspension of N-EtFOSE alcohol into the water. Test substance purity was not characterized. Third party review by Dr. James W. Gillett of Cornell University (observations included with report) also indicate a lack of reliability. REFERENCES_____________________________________________ ________ 3M Technical Report "Evaluation of the Bioconcentration Potential of FM 3422" A. N. Welter, Project 9970612623, Fate of Fluorochemicals, Report Number 2, August 16, 1978 3M requested expert overview, "Bioaccumulation Studies", Dr. James Gillett, Cornell University, March 8,1993 OTHER____________________________________ ,_____________________ Last changed: 5/18/00 006248