Document NGL7Mxon3m9DD4JnkpRjrzxJV

.. N* t ;V , XV r )- v The Tftxlolty of the Vapor ot Arooiorii iEU aaTlIfolt . vv - by . i-. Joseph P, Treon, Ph,D,. Prank P. Cleveland, 11,D. JohnOappel, B,8, Prederlb X. Shaffer, 14 .S. Balph W, Atohley, B.S, ' John?, Torheok, B,S, 'r - V' j ' \ ; , ' /' ' ' ' >X V V- V ' V.v.vr I V. f- . ' J. <. ` June 88, "1955 - -* . THE KETTERING LABORATORY .v. ' ' l\Vh / / v . ~ In tHf - },..> v. ( ' . ' ' . [ ' poportmont of Pfoytnkiva Modictpo and Industrial Haalth ' ;('' N i.. j` Colltga of Madicint UNIVERSITY OF; CINCINNATI, CINCINNATI, OHIO . , %"-) 1 - 1 # i- 1 hons oaaao9 . ./! v . r: Saguenoe of Tables 1. The Mortality, Length of Survival, and Changes In Weight of Animals Exposed to the Vapor of Aroolor 1242 In Air. Experiment No. 1. 2. The Mortality, Length of Survival, and Changes In Weight of Animals Exposed to the Vapor of Aroolor 1242 In Air. Experiment No. E. 3. The Mortality, Length of Survival, and Changes In Weight of Animals Exposed to the Vapor of Aroolor 1234 In Air. Experiment No. 1. 4. The Mortality, Length of Survival, and Changes in Weight of Animals Exposed to Conditioned Air Containing No Vapor of Aroolor 1242 or Aroolor 1234. Experiment No. 1. 3. Summary of the Data on the Mortality Among Croups of Ex perimental and Control Animals. 6. The Average Changes In Weight of Experimental and Control Animals. 7. The Relationships of the Weights of Certain Organs to the Body Weights of Animals Exposed to the Vapor of Aroolor 1242 or Aroolor 1234. t. Summary of the Data on the Relationship of the Weight of the Liver or the Kidneys to the Body Weight of Animals Exposed to the Vapor of Aroolor 1242 or Aroolor 1234. 9. The "Prothrombin Tima" (Method of Kato) of the Blood of Animals Eollovring Their Exposure to the Vapor of Aroolor 1242 or Aroolor 1234 In Air. 10. The "Prothrombin Time" (Method of Kato) of the Blood of Animals Pollowing Their Confinement In Cages Supplied with Conditioned Air Containing No Vapor of Aroolor 1242 or Aroolor 1234. 11. The Effeot of the Exposure of Cats end RAblts to the Vapor of Aroolor 1242 or Aroolor 1234 in Air for 7 Hours per Day on 3 Days per Week: Over a Period of Several Months, Upon the Apparent Prothrombin Oontent (Method cf Kato) of Their Blood* mons oaaaio ii 12, The Effeota of the Intermittent Inhalation of the Vapor of Aroolor 12*15 or Aroolor 1254, or of Conditioned Air, upon the Numbers of Erythrooytea and Iieuoooytes and upon the Conoentration of Hemoglobin In the Peripheral Blood of Animals. 13, The Average Numbers of Erythrooytea and Leuoooytes and the Average Conoentration of Hemoglobin in the Peripheral Blood of Experimental and Control Animals. 14, Applioatlon of the "t" Teat to the Hematologioal Data Ob tained in Observations on Exposed and Oontrol Animals. SONS 088811 Ill Sequenoe of Figures A. Equipment for Volatilizing Aroolor, Chamber and Equipment for Combustion and Collection of Samples. B. Front View of 600-Liter Chamber. C. Humidifier for Furnaoe Sampling Towers. D. Sampling Tovers. X. Standard Curve for the Determination of Aroolor 1242 and Aroolor 1254. 1. Weight of Cat A-446 Recorded at Semi-weekly Intervals During Exposure to the Vapor of Aroolor 1242 In Air S.6 Miorograms per liter (Experiment No. 1), 2. Weights of Individual Guinea Pigs B-331 to B-336 Reoorded at Semi-weekly Intervals During Exposure to the Vapor of Aroolor 1242 in Air - 3,6 Miorograms per liter (Experi ment No, 1). 3. Average and Range of Weight of Guinea Pigs B-331 to B-336 Reoorded at Semi-weekly Intervals During Exposure to the Vapor of Aroolor 1242 in Air - 3.6 Miorograms per liter (Experiment No. 1). 4. Average and Range of Weight of Mioe B-251 to B-260 Re oorded at Semi-weekly Intervals During Exposure to the Vapor of Aroolor 1242 in Air - 3.6 Miorograms per liter (Experiment No. 1). 5. Weights of Individual Rats 1-436 to 1-440 Reoorded at Semi-weekly Intervals During Exposure to the Vapor of Aroolor 1242 in Air - 3.6 Miorograms per liter (Experi ment No. 1). 6. Weights of Individual Rats 1-441 to 1-445 Reoorded at Semi-weekly Intervals During Esp oaure to the Vapor of Aroolor 1242 in Air - 3.6 Miorograms per liter (Experi ment No. 1). muns uaaaiz r 7, Average and Range of Welg&t of Rata L-436 to L-445 Re '' oorded at Semi-weekly Intervals During Exposure to the Vapor of Aroolor 1242 in Air - 8.6 Mlorograns per Liter (Experiment No, 1). s 8. Weights of Individual Rabbits 1-374 to 1-377 Reoorded at Semi-weekly Intervals During Exposure to the Vapor of Aroolor 1242 in Air - 6.6 Miorograms per Liter (Ex- 1 perlment No. 1). 9. Average and Range of Weight of Rabbits 1-374 to 1-377 , Reoorded at Semi-weekly Intervals During Exposure to the Vapor of Aroolor 1242 in Air -6.6 Miorograns per : Liter (Experiment No. 1). 10. VJeight of Oat A-445 Reoorded at Semi-weekly Intervals During Exposure to the Vapor of Aroolor 1242 in Air 6.8 Miorograms per Liter (Experiment No. 2). 11, Weights of Individual Guinea Pigs B-325 to B-328 Re , oorded at Semi-weekly Intervals During Exposure to the Vapor of Aroolor 1242 in Air - 6.6 Miorograms per Liter (Experiment No. 2). ; 12. Weights of Individual Guinea Pigs B-329, B-330 and B-419 Reoorded at Semi-weekly Intervals During Exposure to the Vapor or Aroolor 1242 in Air - 6.6 Miorograms ; per Liter (Experiment No. 2). 13. Average and Range of VIeight of Guinea Figs B-325 to B-330 and B-419 Reoorded at Semi-weekly Intervals Dur ing Exposure to the Vapor of Aroolor 1242 in Air - 6.8 Miorograms per Liter (Experiment No. 2). 14. Average and Range of Weight of Mioe B-241 to B-250, B-376 and B-464 Reoorded at Seml-v/eekly Intervals Dur ing Exposure to the Vapor of Aroolor 1242 In Air - 6.8 Miorograms per Liter (Experiment No. 2). 1 15. Weights of Individual Rats L-526 to L-530 Reoorded at S Sami-weekly intervals During Exposure to the Vapor of k Aroolor 1242 in Air - 6.8 Miorograms per Liter (Experi msnt No. 2). W MGNS 068013 I  16. Weights of Individual Rats L-531 to L-535 and L-d36 Re corded at Semi-weekly Intervals During Exposure to the Vapor of Aroolor 1242 in Air -6.6 Mlorograms per Liter (Experiment No. 2). 17, Average and Range of Weight or Rats L-526 to L-535 and L-^36 Reoorded at Semi-weekly Intervals During Exposure to the Vapor of Aroolor 1242 in Air - 6.6 Mlorograms per Liter (Experiment No. 2) . 1i. Weights of Individual Rabbits I-370 to 1-373 and I-964 Reoorded at Semi-weekly Intervals During Exposure to the Vapor of Aroolor 1242 in Air - 6.6 Mlorograms per Liter (Experiment No. 2) l?. Average and Range of Weight of Rabbits 1-370 to 1-373 and 1-964 Reoorded at Semi-weekly Intervals During Ex posure to the Vapor of Aroolor 1242 in Air - 6.6 Micro grams per Liter (Experiment No. 2). 0. Weight of Oat A-444- Reoorded at Semi-weekly Intervals During Exposure to the Vapor of Aroolor 1254 in Air 5.4 Mlorograms per Liter (Experiment No. 1). 21. Weights of Individual Guinea Pigs B-319 to B-322 Re corded at Semi-weekly Intervals During Exposure to the Vapor of Aroolor 1254 in Air - 5.4 Mlorograms per Liter (Experiment No. 1). 22. Weights of Individual Guinea Pigs B-323, B-324, B-415 and B-4l6 Reoorded at Semi-weekly Intervals During Ex posure to the Vapor of Aroolor 1254 in Air - 5*4 Miorograms per Liter (Experiment No. 1). 23. Average and Range of Weight of Guinea Pigs B-319 to B-324, B-415 and B-416 Reoorded at Semi-weekly Inter vals During Exposure to the Vapor of Aroolor 1254 in Air 5.4 Mlorograms per Liter (Experiment No. 1). 24. Average and Range of Weight of Mioe B-231 to B-240 Reoorded at Semi-weekly Intervals During Exposure to the Vanor of Aroolor 1254 In Air - 54 Mlorograma per . Liter (Experiment No. 1). MUMS 083814 vi 25# Weights of Individual Rats L-299 and L-517 to L-520 Re corded at Semi-weeklv Intervals During Exposure to the Vapor of Aroolor 1254 in Air - 5,4 Miorograms per Liter (Experiment No# 1), 26. Weights of Individual Rats L-521 to L-525 Recorded at Semi-weekly Intervals During Exposure to the Vapor of Aroolor 1254- in Air - 5*4 Miorograms per Liter (Experi ment No, 1). 27, Average and Range of Weight of Rats L-299 and L-517 to L-525 Recorded at Semi-weekly Intervals During Exposure to the Vapor of Aroolor 1254 in Air - 5.4 Miorograms per Liter (Experiment No. 1). 26, Weights of Individual Rabbits I-362 to I-364 Reoorded at Semi-weekly Intervals During Exposure to the Vapor of Aroolor 1254 in Air - 5.4 Miorograms per Liter (Experi ment No. 1). 29. Weights of Individual Rabbits 1-455, 1-567, I-716 and 1-687 Reoorded at Semi-weekly Intervals During Exposure to the Vapor of Aroolor 1254 in Air - 5*4 Miorograms per Liter (Experiment No. 1). 30. Average and Range of Weight of Rabbits I-362 to 1-364, 1-453, 1-567, 1-716 and I-S67 Reoorded at Semi-weekly Intervals During Exposure to the Vapor of Aroolor 1254 in Air - 5*4 Miorograms per Liter (Experiment No. 1). 31. Weight of Control Oat A-435 Reoorded at Semi-weekly Intervals During the Period of Confinement in Oonditioned Air. 32. Weights of Individual Control Quinea Figs B-313 to B-316 Reoorded at Semi-weekly Intervals During the Period of Confinement in Oonditioned Air. 33* Weights of Individual Control Quinea Pigs B-317. B-316, B-402 and B-576 Reoorded at Semi-weekly Intervals During the Period of Confinement in Conditioned Air, 34, Average and Range of Weight of Control Quinea Pigs B-313 to B-316, B-402 and B-57o Reoorded at Semi-weekly In tervals During the Period of Confinement in Conditioned Air. HONS 088815 Tit 35, Average and Bangs of Weight of Control Mine B-221 to B-230, B-4991 B-531* B-532 and B-533 Recorded at Semi weekly Intervals During the Period of Confinement in Conditioned Air. 36, Weights of Individual Control Bats L-507 to L-512 Reoorded at Semi-weekly Intervals During the Periods of Confinement in Conditioned Air. 37, Weights of Individual Control Rats L-513 to L-516 and D-7W Heoorded at Semi-weekly Intervals During the Period of Confinement in Conditioned Air. 36. Average and Bangs of Weight of Control Bata L-507 to L-5I0 and l-744 Recorded at Semi-weekly Intervals During the Period of Confinement in Conditioned Air. 39. Weights of Individual Control Rabbits I-365 and I-366 Heoorded at Semi-weekly Intervals During tbs Period of Confinement in Conditioned Air. 40. Weights of Individual Control Rabbits 1-363, I-369 and 1-975 Reoorded at Semi-weekly Intervals During the Period of Confinement in Conditioned Air. 41. Average and Range of Weight of Control Rabbits I-365 to I-369 and 1-975 During the Period of Confinement in Conditioned Air. HONS 088816 The Toxlolty of the Vapor of Aroolors 124a ana 1854 Soope The purpose of this investigation was to determine the effeots upon animals of various speoies, of moderately pro longed Intermittent exposure to air bearing the vapor of either Aroolor 1848 or Aroolor 1254, This report deals with the re sults obtained in experiments in whioh, for periods ranging up to 4 months, animals were subjeoted to respiratory exposure to air oontaining the Aroolors in oonoentrations that approaohed saturation and so were greater than any likely to be encoun tered in industry. The animals failed to exhibit any signs of lntoxioation during or after the exposure to which they were subjeoted, and the numbers of deaths whioh occurred among the exposed animals were comparable to those whioh resulted from incidental causes in the oontrol groups. Gross and miorosoopio examination of the tissues of the animals of both the exposed and the oontrol groups disclosed the axistanoe of oertaln abnormalities whioh did not differ signifloantly in their severity or frequency of ooourrenoe among the test and oontrol groups. Other experiments whioh Involve more pro longed exposure on the part of animals to lower oonoentrations of theso materials in air, are now in progress. mons oaaax? -2- Summary of Results 1. No signs of intoxication were observed in any of the numbers of a group of animals, all of which survived through out a period of 24- days, on eaoh of 17 of vfoioh they were sub jected to the inhalation of air bearing 3.6 miorograms of Aroolor 1242 per liter (0.33 ppm) ftor 7 hours. The vlsoera of representative animals that were examined miorosooploally were normal. 2. The incidenoe of mortality among the animals of all speoies, excepting the rabbits, in 2 groups that were sub jected (7 hours on eaoh of 32 or 33 days over a period of 120 or 121 days) to the inhalation of air bearing, respectively, Aroolor 1242 in the oonoentration of 6.33 miorograms per liter (0,66 ppm), and Aroolor 1254 in that of *>,4o miorograms per liter (0.4l ppm), v/as no greater than that among a similarly oonstituted oontrol group. Two of 5 rabbits died following exposure to the vapor of Aroolor 1242, as did 3 of 7 rabbits exposed to the vapor of Aroolor 1254, whereas only 1 of 5 of the oontrol rabbits died. The survivors, with the exoeption of the guinea pigs exposed to the vapor of 1254, grew equally as well ae did the oontrols. The average weights of the livers and kidneys (relative to body weights) of the animals eg>osed to Aroolor 1242, did not differ significantly from those of the MONS Oct a 8 Id -3- unexposed group, but the livers of the rats that were exposed to Aroolor 1254 were enlarged. The prothrombin oontent of the blood of the animals exposed to either Aroolor, as measured by the method of Kato, remained within normal limits. The deaths that ooourred among the test and oontrol ani mals were the apparent result of an appreoiable incidence of pneumonia. The lesions of certain of the animals were those of a frank pneumonia; in others such lesions were not fully de veloped. Degenerative changes in the visoera were usually found, in varying degrees of severity, in association with the pneumonia, but in oertain animals, test and oontrol alike, the degenerative ohanges were more evident than the pneumonia. The similarity of the lesions in test and oontrol animals, and the laok of oharaoteristio evidence of ohemioal pneumonitis, lead to the reasonable,but not altogether oertain conclusion that all of these fatalities resulted from interourrent disease among the animals, and not from the effeots of their exposure to the Aroclors. For practical purposes, this oonolusion is subjeot to the critique of further experiments involving more prolonged exposure of animals to somewhat lower concentrations. Properties of the Experimental Materials Aroolor 1242 is a light straw-oolored, mobile liquid with the composition of a ohlorinated biphenyl. A letter from mgns oaasi9 - 4- p.O. Benlaus, dated August 30, 1953* states that It oontalns 42.0 +0.5 Pr oent of ohlorlne, which corresponds to the presenoe of 3 ohlorlne atoms. The positions of the chlorine atoms within the moleoule have not been determined. Monsanto Application Bulletin No. P-115* gives its specific gravity at 15*/25* as 1.373 to 1.333, and states that it has a distilla tion range of 325* to 3^0*0, a refraotlve index (D-llne at 20*0) of 1.627 to 1.629, a Saybolt Universal visooeity of 100r of 30 to 93 seoonds, and a flash point (Cleveland Open Cup) of 176* - l3o0. Its vapor pressure is about 4 mm at 150C and about 30 mm at 200C. Although insoluble in water and glyoerlne, it is soluble in most organlo substanoes. At 745 mm of sroury and 25*0, the oonoentration of 1 mg of the vapor of Aroelor 1242 per liter of air is equivalent to 96.9 ppm by volume. Aroolor 1254, which corresponds to pentaohloroblphenyl, la a light straw-colored, vlsooua liquid. The positions of the ohlorlne atoms have not been establiehed. The moleoular weight of pentaohloroblphenyl is 326.445, of which 54.3 per oent ia ohlorlne. Benigus (ibid) reported that Aroolor 1254 oontalned 54.0 0,5 per oent chlorine. Bulletin No. P-115, states that Aroolor 1254 has a speoifio gravity at 25#/25*C of 1.533 to 1.543, a distillation range of 365* to 39"0, * refraotive Index (D-llne at 20*0) of 1.639 to 1.641, and a Saybolt Universal 088820 visoosity at 100*7 of l,f$00 to 2,500 seooada. The vapor pres sure at 150*0 la about 1*3 an and at 200*0 la about 9 am# It la soluble In most organio aubatanoea, but It la Insoluble In water and glyoerlne. At 745 am of meroury and 25*0 the oonoentration of 1 mg of Aroolor 1254 per liter la equivalent to 76.5 ppm by volume. Experimental Methods Exposure to the Vapor. In a preliminary experiment with the vapor of Aroolor 1242, a group of animals, consisting of a oat, 6 guinea pigs, 10 mioe, 4 rabbits and 10 rats, were oonflned for 7 hours on each of 5 days per week In a rectangular plywood ohamber (volume 600 liters), of whloh the inner metal lining was ooated with a baked ohemically resistant plastic, through whloh was passed a stream of air laden with Aroolor 1242 In a known oonoentration. The stream of air, conditioned with respeot to temperature (75 1 30? Inside the ohamber), dust and humidity, entered the ohamber through an "Anemostat," located at the oenter of Its top, at the rate of 500 liters per minute, as measured by an inollned manometer attaohed to a venturimeter (Figure A), The air was withdrawn by suotlon from the ohamber through an exit tuba located on the rear wall near tbe floop, equidistant from the sides. MONS ousazi -6- Aroolor 1242 was volatilized from a heated glass well main tained at 132* to 13<J*C, The air passed over the surfaoe of the liquid before entering the ohamber (Figure B). In a seoond similar experiment with the same Aroolor, the temperature of the liquid was kept at 100* to 105*C. l.hile the seoond experiment with Aroolor 1242 was in progress an experiment involving the vapor of Aroolor 1254 was oarried out in a seoond ohamber similar to the first exoept for the absenoe of a plastlo inner lining. The rate at whloh air flowed over the liquid Aroolor 1254, which was maintained at 130* - 135*0, was 400 liters per minute. In order to aooustom the animals to the experimental pro cedure, they were kept in their respeotlve ohambers for 7 hours on eaoh of 4 oonseoutlve dayB during the week prior to the in troduction of the Aroolor vapor into the ohamber. For purposes of oontrol, a third group of animals was oonflned throughout a Ilka period before and during the period of the exposure to the Aroolors in another ohamber supplied only with conditioned air. Method for the Determination of Aroolor 1242 or Aroolor 1254 in Air. These materials were determined quantitatively by virtue of the faot that, on thermal decomposition, they yield hydroohlorio aoid whioh, with silver nitrate, forms a suspension, the density of whloh oould be measured by means of the Beokman speotrophotometer at 500 iqu. HUNS 088822 On each day, 2 samples of air from eaoh respiratory ohamber were oolleoted by passing air at the rate of 1 liter per minute for 15 minutes through a Y.'illson oombustlon furnaoe, and then through 2 midget bubblers, in series, eaoh oontaining 10 ml of 0.1 N sodium hydroxide. A quartz tube (13 mm outside diameter, 7 mm inside diameter and 16.25" in length) oontained several strips of folded platinum foil. The middle 9" of the tube was wrapped with a heating unit. The heating unit of the Vllllson furnaoe (Figure C) consisted of 20 feet of B. and S. gauge 22 nlohrome wire (1 ohm per foot), and was oovered with asbeatoa oement. The sample of air was humidified by a ootton wiok saturated with water plaoed 0.5 lnoh upstream from the quartz tuba. With the furnaoe maintained at {J50*C, the Aroolor was decomposed to free ohlorlne, which reacted v/lth the moisture to form HOI; this was absorbed in the 0.1 N sodium hydroxide in the midget bubblers (Figure 0) equipped vrlth frltted-glaee bubblers (Mine Safety Applianoe Company Ho. 4-3S67). The 0.1 H sodium hydroxide waB prepared by dissolving 4.0 g of pellets (ACS speoifioations 0.01 per oent 01) in about 50 ml of double distilled water. To this was added 10 g of arsenio trioxide (ohloride-free) that had been dissolved In water and filtered through ' hatman No. 42 filter paper. Tha oomblned solutions were diluted to 1000 milliliters. MQNS 088823 - a- The sample was transferred to a 25 ml graduated cylinder to whioh 1 drop of a solution of phenolphthalein was added (1 g of phenolphthalein dissolved in 100 ml of CP methanol). The solution was neutralized with 3N nitrio acid (190 ml CP ooaoentrated nitrio aold diluted to 1000 ml with double-distilled water) and diluted to 23 ml with double-distilled water. One ml of 3N nitrio aoid was added to obtain a pH of 1. After adding 1 ml of a solution of silver nitrate (3 grams of AgNOj diluted to 1000 ml with double-distilled water), the suspension was mixed by inversion. After standing 30 minutes, the trans mission of wave-length 500 mu was determined in a 50 mm oell by means of a Bookman speotrophotometer whioh was set against a corresponding oell containing a reagent blank. The amount of Aroolor was determined from a standardized ourve obtained in a similar manner from known quantities of sodium ohloride. A ourve relating the transmission at 500 mp of a suspension resulting from sodium ohloride over the range of 5.0 to 120 miorograms per 25 ml of final suspension is shown in Figure S. On the basis of 42.0 per oent of ohlorine in Aroolor 1242 and 54.0 per oent in Aroolor 1254, 1 miorogram of sodium ohloride is equivalent to 1.4442 miorograms of Aroolor 1242 or to 1.102& miorograms of Aroolor 1254. MONS 0S8S24 -9- Experimental Result8 Aroolor 1242, Experiment No. 1. On oat, 6 guinea pigs, 10 nloe, 4- rabbits ana 10 rats survived when subjected for 7 Hours on eaoh of 17 days over the period of 24 days to the Inhalation of air bearing the vapor of Aroolor 1242 In the oonoentration of 8.6 miorograms per liter (0.83 ppm, Tables 1 and 5). No signs of intoxication were observed. The weights of the individual animals (oat, guinea pigs, rats and rabbits) have been plotted in Figures 1, 2, 5, 6 and 8. The averages end the ranges of the weights of guinea pigs, aloe, rats and rabbits have been plotted at semi-weekly inter vals in Figures 3, 4, 7 and 9, respectively. Despite slight fluctuations in their weight during the period of exposure, all of the animals, exoept the guinea pigs, gained in weight during the period of exposure (Table 6). The average gains made by the various speoies, expressed as per centage of the initial weights were: oats, 8.1; mioe, 10.3; rabbits, 77> and rats, 2.1 The loss in weight on the part of the guinea pigs averaged 2.0 per oent; most of them were gain ing weight (Figure 2) at the termination of the period of exposure. All of the animals were examined post-mortem, and since gross examination of the vlsoera did not reveal any signifi cant alterations, the tissues of only representative animals mqns oaaazs 10 - (1 oat, guinea pigs, 6 mice, 4- rabbits and 6 rats) were geotioned and examined mlorosoopioally. No abnormalities were found In the visoera of these animals. Aroolor 124-2. Experiment No. 2 (6.63 i) /l), The pertinent data relating to the fate of the Individual animals In the Ex periment No. 2 are presented in Table 2 and summarized in Table 5. This group consisted originally of l oat, 6 guinea pigs, 10 mloe, 4 rabbits and 10 rats, but a few additional animals were used to replaoe those whioh died. One oat, 4 guinea pigs, 6 mloe, 2 rabbits and 6 rats survived throughout their exposure, for 7 hours on eaoh of 62 days over a period of 120 days, to air bearing the vapor of Aroolor 1242 In the concentration of 6,63 miorograms per liter (0.66 ppm). Certain animals (2 guinea pigs, 1 mouse, 1 rabbit and 1 rat) were killed after 65 to 66 periods of exposure for examination. In addition, 1 rat and 2 mloe survived throughout 23, 4l and 56 periods of exposure, re spectively. Seven fatalities occurred as follows: 1 guinea pig after 15 periods of exposure; 3 mloe after 24, 4l and 70 periods of exposure, respectively; 2 rabbits after 15 and 64 periods of exposure, respeotively; and 1 rat after 56 periods of exposure. Exoept in the oase of the rabbits, the inoidenoe of mortal ity among the oontrols was equally as great (or greater, Tables 4 and 5) as that among the experimental animals. One oat, 3 MUNS OSda^o - 11 b1, 3 guinea pigs, 3 rabbits and 6 rats survived, following tbeir confinement, for 7 hours on eaoh of S4 days over a period of 122 days, in a chamber in which the air, conditioned with reapeot to dust, humidity and temperature, oontalned no vapor of either Aroolor. One guinea pig, 1 mouse and 1 rat were killed after 66 (or 66) periods of exposure. Other animals introduced later in the experiment survived throughout the following numbers of periods of exposure, respectively; 2 guinea pigs, 20 and 77; 3 mioe, 14, 14 and 19; 1 rabbit, 19; and 1 rat, .Sloven fatalities ocourred at stated times among the oontrol animals, as follows: 2 guinea pigs, after 7 and 6o periods, re spectively; 7 mioe after 5 59, 6<J, 69, 70 , 72 and (S2 periods, respectively; and 1 rat and 1 rabbit after 42 and 65 periods, respectively. No uniform or suggestive signs of intoxication were ob served among either the experimental or oontrol groups. The weights of the individual animals (oat, guinea pigs, rats and rabbits) exposed to Aroolor 1242 are shown in Figures 10, 11, 12, 13, 16 and 16. The average weight, and the great est and least weights attained by any individual within the groups of guinea pigs, mioe, rats and rabbits, are plotted in Figures 13, 14, 17 and 19, respectively. Comparable data on the oontrola are given In Figures 31 to 4l. mons oaaan - 12 - The surviving experimental animals grew equally as well |or better) as the oontrols (Table 6). During the period of exposure to Aroolor 124-2 (6.83 /)/l), the average gains, ex pressed as percentages of the Initial weights, by the various animals \ere as follows: oat, 22.2; guinea pig% 56.4; mloe 21.2; rsbblts, 505; and rats, 17*6. The comparable findings on the oontrols were: oat, 6,6; guinea pigs, 36.6; mloe, 17.4-; rabbits, 44,5; and rats, 16.6. The weights of the livers and kidneys and the relation ships of their v/elghts to the body weights (expressed as grams per 100 grans of body weight) of the Individual animals that survived following exposure to the vapor of Aroolor 1242, are shown in Table 7, wbloh also gives tbs corresponding data on the oontrols. Comparison by the T' test of the varianoes of the ratloe of the liver or kidneys to the body weights of rats, guinea pigs and rabbits exposed to tbs vapor of Aroolor 1242 (6,83 Y/l), with those of tbs oontrols, revealed no slgnifleant dlfferenoes (P *0,05) exoept In the oase of the livers of the rats. Application of the "t" test to the mean values (Table 6) revealed no significant dlfferenoes between the test and oontrol groups of guinea pigs, rats and rabbits. The apparent prothrombin oontent of the blood, as measured by tbs method of Kato (Am. 7. Clin. Path., 10:14-7, 1940), of mons oasa^a 13 rtalfi animals that had been exposed to the vapor of Aroolor 1248, are given In Table 9* Comparable findings on the oontrols are presented In Table 10 to Table XS the percentile relationships of the olotting power have been obtained arbi trarily by dividing 100 times the average olotting time of the blood of the control animals by the olotting time of the blood of tbe experimental animals on the same day. No diminution in the olotting power of the blood, as a measure of the impairment of the funotion of the liver, was induced by the exposure of the animals to the vapor of Aroolor 124-2 when present In the air In the oonoantratlon of 6.6 mlorograms per liter. Determinations of the numbers of erythrocytes and lsuoooytea and of the hemoglobin oontent to the peripheral blood of indi vidual guinea pigs and rabbits subjeoted to inhalation of the vapor of Aroolor 1242 to the oonoentratlon of 6,6 mlorograms par liter, are given In Table 12, whioh also includes oomparable results on oontrol animals. These values have been summarized to Table 13. Application of the "t" test to differences in the mean values (Table 14) for the experimental and oontrol animals yielded border-line evidenoe of significant differenoes to the numbers of leuoooytea end In the concentration to the hemoglobin to the blood of the guinea pigs, but these were small and of opposite sign (the leuoooytea in the blood of the test animals MGNS 088829 - 14 - era low In number while the hemoglobin content was high) , as 11 as being subject to individual variation, and they oannot be regarded as of physiologioal signifioanoe. Of the 7 animals that died during the seoond experiment with Aroolor 1242, all but 2 aloe were examined post-mortem. The tissues of 2 mioe had undergone significant changes, post-mortem, and were not examined microscopically; gross evldenoe to determine the cause of death was not available. A third mouse (B-244-) was examined and found to have a malignant lymphoma that involved the liver, apleen, kidneys and heart, and therefore its death was attri buted to natural oause. The cause of the death of the guinea pig was uncertain, but the 2 rabbits and the rat were found to have died of pneumonia, and not, apparently, of chemical pneu monitis. Generally, the survivors among the various speoies had normal visoera. However, 2 guinea pigs and 1 rat had degenera tive lesions in the liver and kidneys, 1 guinea pig had aoute lobular pneumonia, and 1 rat had Interstitial pneumonia. Eleven of the animals (2 guinea pigs, 7 mioe, 1 rabbit and 1 rat), that had been confined in conditioned air only, died. The viaoara of 6 of these (1 guinea pig, 4 mioe and 1 rabbit) were examined miorosoopioally. Each of the animala examined had died with pneumonia, in association with degenerative MUMS 086030 - 15 - change* of varying severity in the train, liver and kidneys. Animals of all speoies that survived were examined and found to have normal visoera, but for soattered pneumonio lesions in oertain of the guinea pigs and mloe, and for one oase of oellulitis in a rabbit. Aroolor 1254, Experiment No. l (5.40//I). The pertinent data relating to the ohange in weight and the fate of each in dividual animal during and following prolonged intermittent exposure to the vapor of Aroolor 1254- are given in Table 3 and re summarized in Table 3. The original group consisted of tha same numbers and types of animals employed in the other experi ments. One oat, 3 guinea pigs, 4- mice, 2 rabbits, and 9 rats survived throughout their exposure, for 7 hours on eaoh of 63 days over a period of 121 days, to air containing Aroolor 1254 in the concentration of 5*40 mlorograms per liter (o.4l ppm). Oertain other animals (3 guinea pigs, 6 mice, 1 rabbit and 1 rat) were killed for examination after 66 or more (up to 74) periods of exposure. One rabbit, introduced after the experi ment had been under way for some time, survived, having under gone 33 periods of exposure. Five animals died during the experiment, 2 guinea pigs after 12 and 17 periods of exposure, respectively, and 3 rabbits after 15, 15 and 20 periods of ex posure. Exoept for tha rabbits, the incidence of mortality among tha exposed and oontrol groups was comparable (Tables 4 and 5) No signs of intoxioatlon were noted among the experimental animals. mcns oaaan The weights of the individual animals (oat, guinea pigs, rati end rabbits) are given In Figures 80, 21, 28, 25, 26, 26 end 291 while the average weights of the guinea pigs, mice, rats and rabbita are shown in Figures 23, 24, 27 and 30, respectively. Exoept in tlis oase of the guinea pigs and rats, the animals that survived throughout the period of exposure to the vapor of ^roolor 1254 grew equally as well as the controls (Table 6). During the period of exposure the gains made by the animals of various apeoies, expressed as percentages of their initial weights, were as follows: oat, 22.1; guinea pigs, 40.7; mloe, 6.9; rabbits, 39*7; and rats, 6.9. The lesser gain by the experimental rats may be explained by the faot that they were initially somewhat heavier. The corresponding values for the oostrol animals have been given previously and are also shown in Table 6. The weights of the livers and kidneys, and the ratios of these to the body weights, of the animals that survived through out the exposure are shown in Table 7* Application of the "t" test to the differences in the mean values which characterized the experimental and control animals (Table 6) shows that the weights of the livers of the exposed rats were slgnifioantly greater than those of the controls, the ratio of the liver to the body weight of the former being 5*34 g per 100 g of body weight, that of the latter being 4.15'g/lOO grams. MGNS 0aeS3<! - 17 - The results on the apparent prothrombin oontent or the blood of rabbits and of a oat, following exposure to the vapor of Aroolor 1854 (5-40 V/l) are given In Table 9. Little or no diminution In the clotting power was induoed by exposure to the vapor of Aroolor 1854 (Table 11). The numbers of the oellular elements and the oonoentration of the hemoglobin in the peripheral blood of the Individual animals subjeoted to Inhalation of the vapor of Aroolor 1854 are shown in Table 18 and are summarised In Table 13. The slight apparent lnoreases In the hemoglobin of the guinea pigs and In the numbers of erythrooytes of the rabbits, while statisti cally significant (Table 14), oannot be taken seriously as ex pressions of the effeots of the inhalation of Aroolor 1854. The visoera of 4 of the 5 animals (8 guinea pigs and 3 rabbits) that died after varying periods of exposure to the vapor of Aroolor 1254, exhibited diffuse degenerative ohanges in the brain, liver and kidneys, in association with pneumonia* Animals that survived, following exposure to the Aroolor, were examined post-mortem, and the visoera from representative ani mals were examined miorosoopio&lly. The visoera of the oat and guinea pigs were normal. All of the mloe, exoept one, had normal visoera; the exoeption (B-837) had slight degenerative lesions in the liver. A rabbit killed 1 day after the 66th MQNS 088833 - id - p*rlo4 of exposure, had degeneration of the liver and kidneys, nil pulmonary hyperemia and edema. Otherwise, the rabbits that rtn examined had essentially normal viscera. All of the rats that were examined had degenerative ohanges in the liver, and la 5 of the 10, these changes were found to be associated with pneumonia of low grade. The Aroolors did not appear to aot as primary pulmonary irritants. The pneumonia that was observed in both the experlnental and control animals had the oharaoteristios of an in fectious rather than a chemioal prooess. The toxio degenera tive ohanges which were found in the liver and kidneys of all peoies of animals, in both experimental and oontrol groups, while not associated with pneumonia in all instances, may well heve owed ttieir presenoe to the interourrent disease vdiioh was characterized, ohlefly, Inmost Instances as pneumonia. Dlsousslon The probability of the ooourrenoe of the oancentratione of vapor maintained In these experiments in aotual industrial praotioe is remote, slnoe these values approaoh those of atmosphere saturated with the Aroolors* (The signifloanoe of partioulate matter, in the form of oondsnsed droplets or of pro duo ts of ther mal decomposition, has not been involved in these experiments.) hons oaaaj4 - 19 Sinoe the animals which were killed for examination after if to periods of exposure were found to have degenerative lesions in their visoera whioh oould not, with certainty, be attributed to extra-experimental oauses, these experiments ware terminated and others have been initiated at about 1/3 to 1/4 the concentration. From The Kettering Laboratory in the Department of Pre ventive Uedioine and Industrial Health, College of Medlolne, University of Cinoinnati, Cincinnati, Ohio* Project No* 47* Experimental YJork and Report by: Joseph F. Treon, Ph.D* frank P* Cleveland, H.D. John Oappel, B.S, frederio E. Shaffer, M.S. Ralph Vi. Atohley, B.S. John P. Torbe ok, B.S* June 2, 1935 Direotor 086835 HONS Table 1 The Mortality, Length of Survival, and Changes In Weight of Animals Exposed to the Vapor of Aroclor 12iiZ In Air (Experiment No. 1) Coneent ration VA ppm 8.6 0.85 Duration of 'xposure Identification (hours) Number Initial Weight (Mr) Change In '"eight Expressed as Percentage of Initial Weight During After Period Period of of Exposure Exposure 17 x 7.0 17 x 7.0 17 x 7.0 17 x 7.0 17 x 7.0 17 x 7.0 17 x 7.0 17 X 7.0 17 x 7.0 17 x 7.0 17 x 7.0 17 x 7.0 17 x 7.0 17 x 7.0 17 x 7.0 17 x 7.0 17 x 7.0 17 X 7.0 17 X 7.0 Cat A-l.1.6 2.865 + 8.1 + 0 .2 Onlnea Pig B-351 Quinea Pig B-332 Guinea P1& B-333 Guinea Pig E-33J4. Guinea Pig B-335 -- -10.4 0IK0 ; |:is oM : Si + 1.2 - 3-7 - 2.0 - 2.8 - 1.5 Guinea Pig B-55o 0.685 - 5.0 1.9 House B-251 Mouse B-252 * i-fitoes-- 0.028 + 3.6 + 3.8 - 3-6 Mouse B-253 0.027 0.0 0.0 Mouse B-254 0.026 + 7-7 - 3.8 Mouse B-253 0.021 + 19-0 - 4.8 Mouse B-250 Mouse B-257 0.021 0.021 *+ A19:.0l - 14-3 Mouse B-250 0.028 Mouse B-259 Mouse B-260 0.019 0.025 + 10.5 21.7 - lilt Rabbit 1-374 Rabbit 1-375 "Ooo-- 3.0 * 13-9 4.180 8.4 5*8 Fate . Length of Sur vival After Final Period of Exposure Survived Killed 17 days-- Survived Killed 17 days Survived Killed 17 days Survived Killed 3 days Survived Killed 3 days Survived Killed 10 days Survived Killed 10 flaynSurvived Killed 10 days Survived Killed 17 days Survived Killed 17 days Survived Killed 17 days Survived Killed 3 days Survived Killed 10 days Survived Killed 3 days Survived Killed 10 days Survived Killed 17 days Survived Killed 17 davs Survived Killed 3 days Survived Killed 10 days MOMS 0 8 8 8 3 6 Table 1 - Pane 2 Duration of Concentration Exposure . Identification V71 ppm (hours) Humber OOOOOOOOOO 8.6 O.83 17 x 7.0 Rabbit I-376 17 x 7.0___ Rabbit I-XT7 Rat L--436 Rat L-437 Rat L-I4.38 Rat L-439 Rat L-- Rat L-44l Rat L-442 Rat L-kk.3 Rat T.-li/11 Rat L-445 Change in Weight Expressed as Percentage of Initial Weight During After Initial Period Period Weight of of (kg) Exposure Exposure Fate 4.225 A.kOO 0.227 0.250 0.254 0.207 0.228 0.250 0.240 0.230 0.238 0.254 2.9 * 10. * + 1.8 + 4.4 + 1.2 + 0.5 + 2.2 4- 0.8 4.6 2.2 4. 21*.03 4- 1.8 * 6.8 4- 2.6 4. 0.8 : Vk + 0.4 + 5.6 + 1.7 ; Si 5-9 Survived Survived Survived Survived Survived Survived Survived Survived Survived Survived Survived Survived Length of Sur vival After Final Period of Exposure Killed 17 days Killed 2k davs Killed 3 days Killed 3 day8 Killed 10 days Killed 10 days Killed 17 days Killed 10 days Killed 17 days Killed 17 days Killed 17 days Killed 17 days MQNS 0 8 8 8 3 7 Table 2 The Mortality. Length of Survival, and Changes In height of Animals Exposed to the Vapor of Aroclor 121)2 In Air (Experiment Ho. 2) Duration of Concentration Exposure Identification im _E" (hours )Humber Initial Weight 0) Change In Weight Expressed as Percentage of Initial Weight During After Period Period of of Exposure BxpoaursI Fate 0.66 -XJLiL 7.0 7.0 7.0 7.0 7.0 7.0 JLl 2 x 7.0 lio x 7.0 , 7.0 24 x 7.0 Cat A-likS________ Guinea Pig B-325 Guinea Pig B-326 Guinea Pig B-327 Guinea Pig B-328 Guinea Pig B-329 Gtilnea Pig B-330 Guinea Pig B-li.19 House B-24I House B-242 House B-243 House B-2l(4 2.271 0.447 0 0 0 .m 0 I0 0. 00..00220k 0.023 0.017 - 20.1 0.8 - Survived Survived Died Survived Survived Survived Survived Survived Survived Died Survived Died 82 x 7.0 82 x 7.0 82 x 7.0 la x 7.0 Mouse B-2l(5 Mouse B-2ko House B-2I4.7 Mouse B-248 0.019 0.020 0.021 0.019 + 31.6 + 10.0 + + Survived Survived 9.5 Survived Died Length of Sur vival After Final Period of Exposure Killed 1 day Killed 1 day 1 day Killed 1 day Killed 15 days Killed 1 day Killed 15 days Killed 1 day Killed 1 day 1 day Killed 1 day During 24th per iod of exposure Killed 1 day Killed 1 day Killed 15 days During 4lst per- lod of exposure PCPPPO PMOW 6 C 8 B 9 0 SNOW Concentration V/l ppm 6.8} 0.66 Duration of Exposure Identification (hours) Number Initial Weight (kg) Change In Weight Expressed as Percentage of Initial Weight During After Period Period of of Exposure Exposure Fate 82 x 7.0 Mouse B-2k9 82 x 7.0 Mouse B-250 58 x 7.0 Mouse B-376 --111 x 7.0 82 x 7.75 Mouse B-46k Rabbit I-i76 82 x 7.0 Rabbit 1-371 6k x 7-0 Rabbit 1-372 66 x 7.0 Rabbit 1-373 15 x 7.0 Rabbit L-96k +0.020 35.O 0.020 + 25.0 0.02k ----20..062?k t" 2.600 *-5:| ++ 59*6 51*0 2.585 20.5 2.490 + 40 *8 2.758 + 2.2 + 5.0 0.0 + i4`k Survived Survived Survived Survived Survived Survived Died Survived Died K x T-'O 82 x 7.0 82 x 7*0 82 x 7.0 56 x 7.0 82 x 7.0 82 x 7.0 82 x 7.0 82 x 7.0 82 x 7.0 23 x 7.0 Rat L-526------ ----07220 Rat L-527 0.219 Ret L-528 0.200 Rat L-529 Rat L-53O 0.202 0.213 Rat L-53I Rat L-532 0.193 0.206 Rat 1-533 0.219 Rat L-534 0.227 Rat L-535 0.228 Rat L-836 0.207 i+ 195,-15 + 27.O : in + lk.o + lk.l ++ 19.6 17.2 :5i.l 3.2 + 4.8 + 4.8 Survived Survived Survived Survived Died Survived Survived Survived Survived Survived Survived Length of Sur vival After Final Period of Exposure Killed 15 days Killed 15 days IKilled 1 day Killed das-----Killed 1 day Killed 17 days 1 day Killed 1 day During 15th per iod of exposure Killed 1 day Killed 1 day Killed 1 day Killed 1 day 1 day Killed 1 day Killed 8 days Killed 15 days Killed 15 days Killed 15 days Killed 1 day Table 3 The Mortality. Length of Survival, tad Changes In Weight of Animals Exposed to the Vapor of Arcelor 1234 In Air (Experiment No. 1) Duration of 3Co2aceptrftUon _EEE Exposure (hours) Identification Humber Initial Weight (kg) 5-40 O.Ul 2- r.o aj x 7.0 3 * 7-0 83 x 7.0 15 X 7-0 Cat A-444 SulneaPlg Guinea Pig B-32O Guinea Pig B-321 Guinea Pig B-322 2.320 O.4O2 0.400 O.36O 12 X 7-0 66 x 7.0 x 7-0 ..x-7*Q 74 x 7-0 74 x 7.0 83 x 7.0 74 x 7-0 74 x 7.0 8} x 7.0 66 x 7.0 74 x 7*0 83 x 7.0 Guinea Pig B-323 Guinea Pig B-324 Guinea Pig B-4l5 flulaflft-Pla B-U48 House B-23I House B-232 House B-233 Mouse B-234 Mouse B-235 House B-236 House B-237 Mouse B-238 House B-239 0.022 0.020 0.024 0.019 0.020 0.019 0.020 0.019 0.022 Change In height Expressed as Percentage of Initial Weight During" mfter Period Period of of Exposure Exposure! _ 22.1 7opt * 47.3 70.5 41.1 11.9 - 23.0 + 31.4 + + 36.4 * 55.0 37.5 31*6 39-0 26.3 35.0 + 21.1 9.1 4.5 Fate Survived Survived Survived Survived Died Died Survived Survived Survived Survived Survived Survived Survived Survived Survived Survived Survived Survived Length of Sur vival After Final Period of Exposure Killed 1 day Killed l6 daye Killed 16 days Killed 1 day During 15th per iod of exposure 1 day Killed 1 day Killed 1 day Killed 1 day Killed 1 day Killed 1 day Killed 1 day Killed 1 day Killed 1 day Killed 1 day Killed 1 day Killed 1 day Killed 13 days o' o o d mons oaaa^o Table 5 - Paga 2 Duration of Concent ration Exposure Identification r/i ppm (hours) Number 5.40 0.4l 83 * 66 x 7.0 83 x 7.0 83 x 7.0 15 x 7-0 15 x 7.0 20 x 7.0 33 x 7.0 o3 x 7.0 66 x 7.0 83 x 7-0 83 x 7.0 83 X 7.0 83 x 7.0 83 X 7-0 83 x 7-0 83 x 7.0 83 X 7.0 Moose B-240 Rabbit 1-362 Rabbit I-363 Rabbit I-364 Rabbit 1-453 Rabbit 1-587 Rabbit I-J18 Rabbit 1-887 Rat L-299 Rat L-517 Rat L-518 Rat L-519 Rat L-520 Rat L-521 Rat L-522 Rat L-523 Rat L-524 Rat D-525 Initial Weight (kg) 0.022 i.'42.993 2.729 2.100 6.I24 0.235 0.217 0.224 0.237 0.231 0.235 0.226 0.242 0.200 Change in '"eitht Expressed as Percentage of Initial "elRht During After Period Period of of Exposure Exposure Fate + 23*2 - 0.0 + 38.3 i 1:1 *. 1.4 + 1.6 + 2.1 - 1.5 +X 10.6 4.0 12.8 13-5 - 2.1 :U + 5.0 Survived Survived Survived Survived Died Died Died Survived Survived Survived Survived Survived Survived Survived Survived Survived Survived Survived Length of Sur vival After Final Period of Exposure Killed 15 davs Killed 1 day Killed 1 day tilled 16 days 2 days 3 days 1 day Killed 1 dav Killed 1 day Killed 1 day Killed 1 day Killed 1 day Killed 1 day Killed 1 day Killed 15 days Killed 15 days Killed 15 days Killed 15 days mons oaaa4 ?3G8R0 SNOW Duration of Exposure (hours Hi) 8|i x 7.0 64 */?.o 66 y 7.0 84 2 7.0 .7/5 7*0 8L x 7.0 60 x 7.0 77 x 7.0 20 x 7.0 8L x 7-0 68 x 7.0 68 x 7.0 72 x 7.0 82 x 7.0 Table 4 The Mortality. Length of Survival, and Changes In Weight nf Anlmala Ex speed to Conditioned Air Containing Mo Vapor of nf Aroclor 12ii2 or Aroclor 12SU (Experiment Mo* 1) Identification Number Change In Weight Expressed as Percentage of Initial Weight During After Period Period Initial of of Weight Exposure Exposure (kE)(l) (1) Pate Cat A-439 Guinea Pig B-313 Guinea Pig B-3I4 Guinea Pig B-315 Guinea Pig B-316 Guinea Pig B-317 Guinea Pig B-318 2.388 0.335 4i5 0.380 S:S 0.414 6.6 + 95.2 + 25.7 + 78.7 - 6.3 + 77-1 20.3 15.5 14.5 Survived Survived Survived Survived Died Survived Died Guinea Pig B-402 Mouse B-221 Mouse B-222 Mouse B-223 Mouse B-224 0.469 OJiVi 0.021 0.021 0.022 0.021 53.5 * 29.2 + 14.3 23.8 9.1 0.0 - -14..3 - Survived Survived ' Survived Survived Died Died Mouse B-223 0.021 0.0 - Died Length of Survlval After Final Period of Exposure Unless Otherwise Stated Killed 1 dav . Killed 1 day Killed 1 day Killed 15 days 1 day Killed 15 days During 60th period of exposure Killed 1 day Killed 1 dav Killed IS days Killed 1 day 1 day During 72nd period of exposure During 82nd perloc --------of. exposure------- f<?09BO SNOW Duration of Exposure (hours Xl) 69 x 7-0 59 x 7-0 70 x 7.0 84 x 7.0 84 x 7.0 5 * 7.0 14 x 7.0 l4 x 7.0 19 x 7.0 Bt x 7.0 84 x 7-0 84. x 7.0 65 x 7-0 19 x 7.0 4 x 7.0 84 x 7-0 84 x 7-0 8I4. x 7.0 42 x 7-0 84 x 7.0 84 x 7.0 Identification Buaber Mouse B-226 Mouse B-227 Mouse B-228 Mouse B-229 Mouse B-230 House B-499 Mouse B-531 Mouse B-532 Mouse B-555 Rabbit 1-365 Rabbit I-366 Rabbit I-368 Rabbit I-369 Rabbit 1-795 Rat L-567 Rat L-508 Rat L-6O9 Rat L-510 Rat L-511 Rat L-512 Rat L-513 Initial Weight (IwXl) Change in Weight Expressed as Percentage of . Initial Weight During After Period Period of of Exposure(1] Exposure Pate 0.021 0.0 0.022 . - 4p.9 0.022 - 4-5 Died - Died - Died 0.023 0.023 0.023 0.023 0.015 0.050 3.084 3.162 2.568 3*352 30.4 0.0 - - 4-3 + 84.6 - 26.7 48.1 + 1|B.6 5P-7 14.0 0.0 0.0 - 0.0 0.0 0.0 - -18.0 Survived Survived Died Survived Survived Survived Survived Survived Survived Died 2.243 5.269 0.231 0.212 0.225 0.212 + I4.5 + 2.2 9-9 19.1 - 34-4 - Survived ~-Tnj-- Survived 1- 3.9 3-8 -0.9 Survived Survived Survived Died 0.192 0.203 27.6 4 26.6 0.0 * 1-5 Survived Survived Length of Sur vival After Pinal Period of Exposure tJnless Otherwise Stated 3 days 1 day During 70th period of exposure Killed 3 <*ais Killed 3 days 3 days Killed 3 days Killed 3 days Killed 5 davs Killed 1 day Killed 1 day Killed 15 days During 65th period of exposure Killed 1 day Killed"!^ fays------Killed 15 days Killed 15 days Killed 15 days During 42nd period of exposure Killed 3 days Killed 3 days Table U - Page 5 Duration of Exposure (hours )(1) 8k x 7.0 8k x 7-0 x 7.0 kk * 7-0 Identification Number Rat L-5lk Rat L-S15 Rat L-516 Rat L-7k8 Initial Weight Otg) O.I95 0.217 0.210 0.218 Change In Weight Expressed as Percentage of Initial Weight During After Period Period of of Exposure(l) Exposure 12.8 2k.k 3-3 22.9 0.0 0.0 0.0 Pate Survived Survived Survived Survived Length of Sur vival After Final Period of Exposure Unless otherwise Stated Killed 3 days Killed 3 days Killed 1 day Killed 3 days (1) Computed from time of Initial exposure of experimental animals to the vapor of Aroolor; the original group of controls and the original group of experimental animals were confined -into conditioned air only, on each of four days during the previous week- MONS 0 8 8 8 4 4 Table *> STMry of the Data on Mortality Among Groups of Experimental and Control Animals Material Concentration W1 ppm Aroclor 1242 8.60 O.83 Duration of Exposure (hours) 17 x 7*0 Number of Fatalitles/Humber of Animals Exposed Cats 0/1 Guinea Flics Mice 0/6 0/10 Rabbits Rats oA 0/10 Aroclor 1242 6.83 0.66 82 x 7.0 0/1 1/7 CD (2) 3/12(1) 2/5 U) 1/11 (1)(4) (3) Aroclor 1254 5.40 0.4l 83 x 7-0 0/1 2/8 (1) (2) 0/10(1) 3/7UH7) 0/10 (1) (5) (6) Controls 0 0 84 x 7.0 0/1 2/8 (1) (8) 7/14(2) 1/5(10) lAKD(li) -J21- (1) /One survivor was exposed on only 65 or 66 days. (2) ( One survivor was exposed on only 67 or 66 days. / Two survivors were exposed on only 4l and 58 days, respectively. . One survivor was exposed on only 23 days. (5J One survivor was exposed on only 71 days. (6) Five survivors were exposed on only 74 days. (7) One survivor was exposed on only 33 days. (8) Two survivors were exposed on only 20 and 77 days, respectively. (9) Three survivors were exposed on only 1I4. and 19 days, respectively. 10) One of the survivors was exposed on only 19 days, ll) One of the survivors was exposed on only 44 days. S *r9 9 9 0 SNOW table 6 The Average Changes In Weight of Experimental and Control Animals Survivors Animals That Died Species n Average Change Average In height at Weight Average Termination During Initial of Exposure Height Experiment (1) Peg) fire) ___ ____<Ml_ Average Change In Weight Expressed &8 Percentage 0f Initial Weight (1) n Average Average Change Change in Weight Average Initial Average in Expressed Weight at Weight as Termination During Percentage of Exposure of Weight Experiment (g>___ Kg) , , (1) Initial Weight (1) Group Exposed to 8.6Vof Aroclor 1242/liter (Exp. Ho. 1) Eat 1- '~27HSr~ "T4WK---------- 0.2*2" "'"8.1------- U- Guinea, . Pises 6 0.649 Klee 16 A.62b o.64o O.626 - 0.008 - 2.0 o.o62 + 5*i 0 6 Rabbits 4 5.$<n Bats io 0.2=58 4.19? 6.245 + 0.291 + 0.005 ++ 72*.17 6 6 _ - - - - em - --" - Cal: ~T~ 2.271 Guinea ,rlPlKS 6 0.404 jjlee $ 6.621 RMlbits 5 2.687 Rats io 6.2i2 Group Exposed to 6.83 Vof Aroclor 12J|2/llter (Exp. Ho. 2) ~s:tk---------- + 6.664 T"2272-------- ~TST~ - - 0.629 0.225 6.626 + 6.66b =5.898' ' + I.5T1 "8.W8---------- + 0.058 _56.k____ + 21.2 + + -- 17.6 1 5 2 1 o.}85 6.626 27872"" 6.215 $.521 5.619 27568 0.200 -- - 0.064 - 16.6 '- 6.061 - 1____ 0 . _____ - 0.015 - 6.1 MQNS 08&B*6 Table 6 - Page 2 Species n Survivors Average Average Change Change in Height Average In Expressed Weight at Weight as Average Tendnation During Percentage Initial of Exposure of Weight Experiment U) Initial A*)- ___ iMi______ ..... IteL.. Welfeht, (l].. n Animals That Died Average Initial Weight (kg) Average Average Change Change in weight Average In Expressed Height at Height aa Terminatioi During Percentage of Exposure of Experiment a) Initial (kg) (kg) Weight (1) Cat------- "I 2.T20 ' Guinea Pigs 6 0.1i09 Klee 16 0.021 Rabbits L TT722-- Rata ir~ 0.227 v:m-------- T"o7*nT" -rsiKl-------- 15 - 0.571 O.oif 3.b0b O.iki 0.162 + 6.06b l.ObS + 0.0K + k2ft>A.7 + 3S I + l)#( 2 0.L.02 0 3 27,j59 0 Group Confined in Conditioned Air Only Cat nr- 77*88-- "Z75K---------- v-aiwr 6.6 15 - Guinea Pigs 6 0.U18 Vice ~T~ (T.052 Rabbits A 2.76L olOal lx.612 + 0.227 + 56.6 + 6.6o2 4 iV.il + 1.211.B + uu. 2 & 20*.65222? 1 ! -352 Rata 16 6.211 0.2L6 + 6.03L + 16 .t 1 6.212 - 0.277 - 2*^2 6.620 3.U22 o-iil -- - 0.125 - 32.1 - - O.hlb - 17.h - . -- 0.050 ___ IsO_______ - 6.661 - 6.1 0.1i70 ETC 1 - 6.673 - " CO(1) Pinal Weight - Initial Height x 100* ------- initial height* " >B880 SNOW Table 7 The Relatlonahlpa of the Weights of Certain Organa to the Body Weights of Anlmala Exposed to the Vapor of Aroclor l2h2 or Aroclor 1254 Liver Kidneys Identlfl- eatlon Number Ratio of Weight of Number of Body Perioda of Weight Organ x 100 to Body Exposure lk). _a___ Weight Ratio of Weight of Organ x 100 to Body JS__ Weight Aroelor 121+2 6.83 Mlorogran s/liter Cat A-445 ------82--------- W 92.6 Guinea Big B-325 82 639 23.9 Guinea Fig B-527 82 798 31-4 Guinea Big B-328 82 609 31.0 Guinea Fig B-329 66 102 22.0 Guinea Pig B-330 82 698 39.0 Guinea Pig B-419 Average 67 7) $3 34.0 bkO 29.6 Rabbit 1-370 BKaIFbEbTitFTT-^J7[213___ Average 02 82 66 77 4265 itWO 390b G025 112.0 i44-6 110.0 122 Iff 3735 W ' 0.631---------- 3.74 3.4 3.93 . 5.09 . _ 4-? 6.0 5-34 _____ 9.01 4-5 _ 6.2 0.532 o.6i4 0-985 1.092 0.888 t1..98 4l.bO . 49 9.0 2753 14.5 TPfr jOk________ .Oh Tftfr lo *6 p(3..781679 0.340 6.560 6.565 0.4bo Identifi cation Number Tabla 7 - Page 2 Liver Number of Body Periods of Height Exposure --s____ Ratio of Weight of Organ x 100. to Body Weight Kidneys ,.s..... Ratio of Weight of Organ x 100 to Body Welrht HONS 0 8 0 8 4 9 Cat A-lihli Guinea Pig B-519 Guinea Pig B-320 Guinea Pig B-321 Guinea Pig B-32k Guinea Pig B-U15 .. 5 . 83 366 83 6ko . 83 682 66 510 ____ 21______ h.62 93.3 -------------- rETC------ ---------------- 22.9 k.03 U.6 0.813 27.3 26.3 26.0 -22:?-, U.27 3.86 5-10 5.02 5.2 .. 0.813 U.2 0.6l6 U.8 0.9U Jti____ 0.866 Identifi cation Number Table 7 - Page 3 Liver Kidneys Number of Body Periods of height Exposure _isl___ __ g..... Ratio of Ratio of Weight of Weight of Organ x 100 Organ x 100 to Body to Body Weight _s___ Weight O S P P P 0 SNOW <jaV"A-n^'" -f-------be------ i?&t--r~LB.; -| "ivgi.........f nr.l ~ | "p;?gg Identifi cation Number Liver Ratio of Weight of Number of Body Organ 1 100 Periods of height to Body Eaposure ____tl_ __ &___ Weight Controls g Ratio of Weight of Organ x 100 to Body Weight Guinea Pig B-313 84 ^.k -- 25.1 3. Bit . 4..6,,_ 0.703 Guinea Pig B-314 U 519 18.2 ______ 4.6 0.886 Guinea Pig B-315 8k 738 36.4 4-93______ 4.0 o-,5kg_____ Guinea Pig B-317 8k _ 870 lt0.8 It. 69 5.7 0.695 Guinea Pig B-h.02 77 626 29.7 . -4-I4______ . 4r9, - - 7.8.3_____ Guinea Pig B-576 Average 20 b9 588 _ 666 31.3 5-3?______ 4.8 0.816 4.51 It*8 "U7T3I Rabbit' 1-365 "i Bit 4505 Rabbit I-3bb Bit ... 5606 Rabbit 1-368 Oii 3490 Rabbit 1-975 1? 2973 Average --m---------- 3993 lilt.o ioli.6 ..aSak. _____ 2__._O_B_______ _____2J4_______ 1B.B .2g1O.jBjj - ... Qo.-44o1bI_____ 57529 85.7 2788 lb-? 0*56o 102.3 2751 19.5 0.564 Rat L-507 Rat L-50B 8h 203 B4_ 245 8.2 .8.4 4.p4_______ 1?7._ __ 3.43 i7. . Q.-W&. .. _ Rat L-509 Rat L-SiO Bit 2ltl r~ 266 9.5 10.9 3.?4 . _____ iS._______ za-. - -O'224_____ 1.9 6.714 T S B P 8 0 SNOW Identifi cation Number Rat L-912 Rat Rat Rat L-515 Rat iL-ffiRafc Average- Table 7 - Page S Liver Number of Periods of Exposure Body Weight Ratio of Weight of Organ x 100 to Body __ S______ Weight Controls III ~87T 16."2 270 ~22i6rltTp MU Kidneys Ratio of Weight of Organ x 100 to Body -g Weight ilx.Ji. 276 0.890 m. "6.867 Aroclor 1242 1242 12J+2 1254 1254 1254 1242 1242 12l2 1254 1254 1254 1254 1254 1254 1254 1254 1254 Organ Liver Liver Liver Liver Liver Liver Kidney Kidney Kidney Kidney Kidney Kidney Liver Liver Liver Kidney Kidney Kidney Speclee n Rat Guinea Pig l Rabbit Rat 3 10 Guinea Pig 6 Rabbit 4 Rat 9 Guinea Pig 6 Rabbit Rat 5 10 Guinea Pig 6 Rabbit Rat 4 10 Guinea Pig 'b Rabbit Rat 4 10 Guinea Pig Rabbit 4 Ratio of Average Average height of Organ Body Organ x 100 '/eight V'eight to Body ---IgL.----- -Jgll- V/elaht 1172 240 29.6 640 122.0 12.8 25.9 120.0 1.85 5.0 l8.6 2.1 4.6 4023 239 581 355? 240 64o 4023 239 581 3.04 5-34 4.51 3-53 0.773 o-aos 0.468 0.770 0^06 15.8 9.0 3537 0.464 4.15 50.3 102.3 2.0 4.8 5993 66^ 4-51 2.61 0.807 O.73I 19-5 3995 0.504 t 1.231 0.4291 I.5C85 3*3992 0.0000 1.0122 0.5842 0.7257 o.4l?7 0.4199 0.5411 0.3163 - - - p --0V20-0.30 ^0.50 0.10-0.20 <0.01 >0.50 >0.50 >0.50 0.40-0.50 - >0.50 >0.50 >0.50 >0.50 - - - - MONS 088853 t rx - i2 n^niTn + na - 2) (nx + ns){Sx| + Sig) df = n! + na - 2 <><;89R0 SNOW Date 9/11/55 mi 10/2U/33 n/iii/53 12/5/53, WA Pa "Prothrombin Tint"; (Method of Kto) or the Blood of Anlwala Following their Expoaurt to the Vapor or Aroclor 12U2 or Aroclor IZ^li In Air Clotting Time, Seconds Experiment Ho. 2 Aroclor 1242 - 6.83 Yj\ Cat A-445 20.00 18.50 18.00 16.50 17.00 19.00 17.00 23K 19.00 17.00 13.50 ` .00 .00 5 16.00 Rabbits T17T 19.50 17.00 15.OO 15.00 13.50 15.OO 17.00 15.00 19.50 18.00 13.50 14.50 13.50 16.00 I-3?3 18.50 16.50 13.50 14.00 i4.oo 16.75 16.75 O2 2 C/> SSB8B0 rttm"frotiu-o^i n T "-* t|,ittl,,<J<*1' r^tn) of ttv. rir>cKl or rollowlM Tn.lr Conflnwnt In Ctftte ouddIlot? wltn Conditioned Air Cunulnlim Mo Vipor ot Aroclor 12U2 or Aroclor 12SU Clotting Tine, Seconds Date 9/11/53 9/12/53 9/26A3 10/24/$3 11/14/53 12/5/53, mink Cat A-k35 20.00 20.00 16.25 13.75 17.25 13.50 19.75 18.00 I-3bS 16.00 16.00 15.25 13.50 14.50 15.50 16.75 15.50 1-566 16.00 16.50 13.50 14.50 13.00 16.25 15.50 16.00 Rabbits 1-568 16.00 16.00 is. 50 14.50 13.50 15-75 17.75 14.25 15.00 1-369 lS-50 16.25 14.75 15.00 13.00 17.00 1-975 17.25 14.00 Average 16.63 16.19 13.50 16.13 16.81 14.94 15.00 9SP0PO SNOW iseeso The Effect of the Exp or Aroclor lZ^li ln~A lod of Several Months, Upon ' TM 11 Relation of "Prothrombin Time" of Control and Exposed Animals Expressed Arbitrarily in Terms of Percentage Change in Clotting Time of Blood 100 x Clotting Time of-Blood of Control Animals Clotting Time of Blood of Exposed Animals Experiment No. 2 Aroclor 12i|2 - 6.8; f/1 Time of Sampling in .Relation to Period of Eiposnre Days Before First Period Serial riumber of Per iod After Which Blood Was Drawn TtL 11 - ry- r Relation of "Prothrombin Time" of Control and Exposed Animals Expressed Arbitrarily In Terms of Percentage Change In Clotting Time of Blood 100 x Clotting Time of Blood of Control Animals_______ Clotting Time of Blood of Exposed Animals ITlma of Sampling In Relation to Period of .Exposure Days Before First Period Serial Num- ber of Perlod After Which Blood Was Drawn Experiment No. 1 Aroolor 1254 - 5 *4 `Yl'L Cat A-lilJi Rabbits 1-362 1-363 1-453 T-5H7 1-710 1-007 Average 100.0 100.0 98.5 113.6 101.5 92.5 90.0 97.8 101.2 89.1 95-9 90.0 93-5 92.3 98.1 89.1 102.7 96.L 97.5 100.4 97.8 89.9 95.0 95.9 100.0 97*8 100.0 97*8 95.2 78.1 102.7 ' 96.4 81.8 112.1 B 90.0 99.6 102.0 112.1 97.0 95-4 95-2 100.0 12 9 10 15 30 ?2 45 03 (2) PSflfifiO SNDW H 1 (2) Determination made lit- days after 83rd period of exposure Table 12 The Effects of the Intermittent Inhalation of the Vapor of Aroclor 12L2 or Aroclor 12.I. r of Conditioned Air, tlpon the numbers of Erythrocytes and Leucocytes anJ~lTpon the Concentrm- tlon of Hemoglobin In the Peripheral Blood of Animals Experiment Ho. 2 Aroclor 1242 - 6.83 V/l: 7 hours/day Identifica tion Number Cat A-445 Dates of First and Last Period of Exposure 9/14/53 1/11/54 Guinea Pig B-325 9/14/53 i/n/54 Guinea Pig B-326 9/14/513 Guinea Pig 10/2 /*v5 9/14/53 ' 1/11/54 Date of Determi nation 1 12/22/53 Average 9/18/53 10/29/53 12/21/53 Average 9/16/53 9/16/53 10/29/53 12/21/53 Average Hemoglobin g/100 ml 12.4 10.3 10.0 10.9 13-8 S3 14.5 15.8 14.6 15.8 15.8 15.4 Erythrocytes (Thousands) per mri5 8,0S0 7,18 7,180 7,470 5,480 6,540 6,o4o 6.010 5,830 5,26 6,620 6,030 5,970 Leucocyte* per jsa3 20,950 12,700 15,050 16,218 16,350 10,300 11,200 l26l7_____ 16,750 6,400 11,300 8,200 8,633 ----- ` 6 S 8 8 8 0 SNOW moms oaaaco Table XZ - 2 Experiment Ho. 2 Aroclor 121+2 - 6.837^1; 7 hcmre/dmy Identifica tion Number Guinea tig 6-328 Dates of First and Last Period of Exposure 1/14/53 Guinea Pig B-32 1/11/54 9/14/53 giilnea-yig~B=?3G' 12/15/53 9/14/53 Guinea Pig 6-419 Rabbit 1-170--------- 1/11/54 16/5753-- 1/11/54 9/14/53 1/11/54 Date of Determi nation 9/16/53 10/29/53 12/5/53 Average 9/16/53 10/29/53 12/12/53 Average 9/18/53 10/29/53 12/21/53 Average 12/21/53 9/16/53 10/28/53 12/22/53 Average Hemoglobin g/100 ml . 12.7 13-1 13.4 13-1 15.0 13-1 14.2 14.1 12.0 15.4 13.8 13-7 . 14.2 11.6 13.4 12.7 12.6 Erythrocytes (Thousands) per mm3 5,290 5.550 5,620 5,487 5,150 5,500 5,420 5,357 4,930 6,370 5,740 5.680 5,570 5.T&0 6,900 5,590 6,090 Leucocytes per mm3 lg,30 8,150 15,850 10,767 5,700 9,150 11,950 5,600 6,500 8,300 10,000 8.267 8,150 9.400 15,400 10,600 11,800 T9RBR0 SNOW Tbl iz JKag--a Experiment No* 2 Aroclor 1242 - 6.83 v7l: 7 houra/flay Identifica tion Humber Dates of Plrat and Last Period of Exposure Rabbit I-3?l-------- 9/14/53 - Rabbit 1-372 1/11/54 9/14/53 Late of Determi nation 9/16/53 10/23/53 12/22/53 Average Rabbit 1-373 Rabbit 1-964 12/11/53 9/14/53 12/15/53 12/15/53 1/7/54 Avarafe 9A6/53 10/28/53 12/9/53 Averaee 12/22/53 Hemoglobin g/100 ml 12.0 11.4 12.4 11.9 11.6 13.1 12.4 11.6 11.6 13.1 12.1 11.6 . . Erythrocytes (Thousands^ per mm?* 5,900 5,940 6,030 5,957 5,970 7,090 . . 6.530 5,54 6,o4o 5,i4o 5.573 6,290 Cat A-l<l)4 Experiment Ho. 1 Aroclor 1254 - 5*40 V/l: 7 hours/day 9/14/53 9/16/53 11.6 9,540 Leucocytes per an* 12,250 12,300 7,350 10,633 _ 8,950 14,000 _ 11,475_. 8,150 1-0,450 8,600 9,067 5,800 25,900 MONS 008862 Tbl \2 - PK. k Experiment Ho. 1 Aroclor 1254 - 5.40?'7,ls 7 hours/day Cates of Identifica tion Humber First and Last Period of Exposure Date of Determi nation Cat A-lM 10/30/53 12/23/53 1/12/54 Average Guinea Pig B-319 9/14/53 Guinea Pig B-320 Guinea Pig B-321 Guinea Pig B-322 Guinea Pig B-323 12/22/53 1/12/54 Averase 9/14/53 9/21/53 11/2/53 12/22/53 1/12/54 Average 9/14/53 1/13^54 9/21/53 H/2/53 12/23/53 Averaee 9/l4/53 10/2/53 9/21/53 9/W53 | 9/29/53___ 9/21/53 Hemoglobin g/100 ml. 12.0 13.4 12.3 15.0 15.0 13*4 14.5 14.6 15.8 13.8 14.7 14.6 14.6 15.4 14.9 13.4 15.0 Erythrocytes (Thousands) per mP 7.550 8.493 5,780 5.970 5,560 5.777 5,760 5,690 5,310 5,587 5,450 6,190 6,170 5.937 5,760 6,270 Leucocytes per rmP 25,100 26,350 25.783 16,000 9,550 11,350 12.300 12,100 8,550 16,550 12,400 6,35 16,650 12,100 11.700 14,950 8,700 T*ble 12 * Pmr.m ^ Experiment No. 1 Aroclor 1254 " 5*4oYyi; 7 tiours/day Identifica tion Nnmher Guinea Pig B-324 Bates of First and Last Period of Exnosure *97*5757" Guinea Pig B-I4.I5 12/15/53 9/30/53 Guinea Pig B-I4.I8 1/12/54 10/5/53 Rabbit 1-362 1/12/54 --9755753 Rabbit I-363 12/15/53 --9/55753"" Bate of Betermi- natlon 9/21/53 11/2/53 12/12/53 AvAT*AA mb ivories 11/2/53 12/22/53 AveraRe 9/18/53 10/30/53 12/9/53 Averace 9/18/2 1/12/54 Average Hemorlobin g/100 ml 15.0 13.8 14.2 14.3 14.2 14.6 14.4 14*6 14.2 14.4 14.2 13.4 13.1 13.6 13-4 11.6 11.6 12.2 Erythrocytes (Thousands) per mm3 Leucocytes per mm3 5,880 6,200 6,110 15,200 6.061 15.167 5,930 5,710 22.900 12.900 ... S.820 6,330 5,410 . 17 AQ2. 10,000 10,600 5,870 .. 10.300 6,850 6,020 5,960 15,300 12,400 13,100 __ ------------------- 6,770 5,960 5,470 13.600 7,400 10,450 12,500 6,067 10,117 C 9 B B 8 0 SNOW i HONS 088S6<* Identifica tion Number Rabbit I-3&4 Rabbit 1-453 Rabbit I-587 Rabbit 1-718 "Rabbit I-887 Experiment Ho. X Aroc lor 1254 - 5.1*0V/l 1 Dates or First and Last Period of Exposure 9/14/53 1/12/54 9/14/53 10/2/53 10/5/53 .10/23/53 10726/53 11/20/53 h/23/53 1/12/54 Date of Determi nation 9/18/53 10/30/53 12/23/53 Average 9/18/53 10/30/53 12/23/53 Hemoglobin g/100 ml 13.4 12.4 12.4 12.7 15.0 11.6 15.8 7 hours/iUy Erythrocytes (Thousands) per nn? 6,43 6,890 6,300 6.sko 6,900 6,500 6,630 car A'-435 ' ---------- 578753-- 1/13/54 Conditioned Air 9/22/53 11/3/53 12/23/53 Average 10.0 10.6 11.0 10.5 7,970 7,140 7,200 7,437 Leucocytes per mm* 6,050 13,800 10,000 11,050 10,850 9,500 25,050 14,450 12,350 17,283 MONS G 888& 5 Identifica tion Humber Guinea Pig B-313 Dates of First and Last Period of Exposure 9/3/53" . 1/13/54 Guinea Pig B-314 ----- 978753" 12/15/54 Guinea Pig B-3l5 --5/8/53 1/13/54 Guinea Pig B-31b --578753 9/22/53 Guinea Pig B-317 9/8/53 1/13/54 Date of Determi nation 9/23/53 H/4/53 12/23/53 Average 9/23/53 1112//142/5/533 Average 9/23/53 11/4/53 12/23/53 Average Hemoglobin g/100 ml 13.4 13.8 15.4 14.2 12.0 14.2 -- 13.1 3.3.8 I4.6 14.2 14.2 9/23/53 1W53 12/23/53 Average ! t i 13.4 15.0 15.0 14.5 Erythrocytes (Thousands) per mm? j j j Leucocytes per mm 5.49 6,080 5,870 j 11,250 ; 15,050 11,150 ____ ^813__________ 5,29 5,780 5,230 12,453_ 12,15 14,800 19,250 5.433__________ 15,40Q-- 5,790 5,510 5,540 14,950 17,050 17,050 5,113__________ _______ 15,867 _ 5,970 6,980 6,590 6,513 6,000 5,45 10,450 7,300 990PPO SNOW Table 12 - Page B Conditioned Air Identifica tion Guinea Pig fe-jld Dates of First and Last Period of Exdosuts i/mi-- Guinea Pig B-402 12/4/53 ' 9/23/53 Date of Determi nation 9/23/53 nA/53 Average 4Y? Guinea Pig B-576 Rabbit I-365 1/13/54 12/14/53 1/13/54 --mm-- 12/23/53 Average 12/23/53 Rabbit 1-366 1/13/54 9/8/53 1/13/54 12/23/53 Average 9/22/53 12^23^53 Average Hemoglobin g/100 ml. 13.4 12.7 13.1 . 15-1 13.8 15.4 14.1 14.2 11.4 12.4 13.4 12.4 11.4 11.4 12.7 11.6 Erythrocytes (Thousands) per mm3 5,520 5,320 5,420 5,320 5,210 5,830 5,453 5,860 5,900 5,800 6,200 5.967 5,860 5,760 5,280 5,633 leucocytes per nan3 9,900 17,000 13,450 10,100 10,650 12,150 10,967 9,400 8,900 8,100 11,450 9,483 _ 16,400 16,250 13,750 15,467 Identifica tion Number Rabbit I-368 Rabbit 1-369 Rabbit 1-975 Dates of First and Last Period of Exposure 9/B/53 1/13/5* 9/5/53 12/14/5* 12/15/53 1/13/5* Table 12 - Page 9 Conditioned Air Date of Determi nation 9/22/53 11/3/53 12/23/53 Arerage 9/22/53 H/3/53 12/9/53 Average 12/23/53 Hemoglobin g/100 ml 11.1*. 11.4 12.4 . u7 12.4 12.0 11.6 12.0 13.1 Erythrocytes (Thousands) per mna 5,630 5,060 6,240 5,643 6,490 5,900 5,260 5,883 6,420 Leucocytes per mm3 9,100 10,900 13,100 11,033 11,700 9,100 16,300 12,367 9,600 TM> 1* The Avartae Wuyberi of ErTthroCTtya and LaucocH and the Average Concentration of HewoiXobln In the Peripheral Blocd of Experimental and Controt inline la Erythrocytes: 3 Thousands per mm Leucocyte*: per mm3 Hemoglobin: g/100 ml Species of Animals Average X n Average X n Experiment No. 2 Aroclor 121+2 - 6.83 V/l.: Average X 7 hours/day n Guinea Pigs Rabbits Guinea Pigs Rabbits 37731------ 7 b!o88 ____ 5 ...1h0,i1m15_(_ilLl _57_--_-_--_-_- ---------- U+.1+'T2)'12.1 ----- 7-----___ 5____ Experiment No. 1 Aroclor 125l+ - 5*1+0 V/l.: 7 houra/day 37BBS---------- ------8----------- 6.1+86(2) 6 IZ7P7 10.811 0 ---------- 143(2)...... ------- 8--------- 6 13.2_______ 6 Controls Exposed to Conditioned Air Guinea Pigs x Rabbits C2l 37759" 5,909 7 ----------- 157151+-- 7 5 11,590 5 13.9 12.2 7 5 (1) Value significantly less than that obtained on controls. (2) Value significantly greater than that obtained on controls P9BBB0 SNOW TtbU Ha Application of the "t" Test to the HwmtolQKlctl Data obtained In bbscrvat^.ona on Exposed and Control Anl'malB 6 9 9 8 9 0 SNOW <+ Species of Animals Erythrocytes thousands /mm3 af It 1 r Leucocytes Per mm Experiment No. 2 Aroclor 12l*2 - 6.83 v/l; Hemoglobin 6/100 ml df | t IP 7 hours/day Guinea figs T2---------0. look------- >0.50 12 Rabbits 8 0.8298 0.40-0.50 8 2.709410.01-0.02 12 1.4571I0.10-0.20 8 3.3439 0.5086 <73701 ____>P-i5Q_ Experiment No. 1 Aroclor 1254 - 5.40 *71; 7 bours/day Guinea Pigs 1 13 Rabbits 19 1 0*9921*. 0.30-0*40 13 0.5117 1 0,01-0.02 _2____ 0.6457 >0.50 >0.50 13 ^.1751 _2___ 1.6425 0.02-0.05 0.20-0.10 t = Xx- xs lining (n^ + n8 - 2) . df - m + nz - 2 l| (ni + n2) (Sxjt + Sx|) 8.6 Mlorograma of Aroolor 1242/llter Figure 1 MGNS 088870 mows oaaa7i Time, Weeks I.r.rcrlsKmt No. 1 t).6 Mlorograma of Aroclor 1242/liter Figure 3 MGNi uood Mloe B251 to B260 29 28 27 26 25 Zk 23 22 21 20 19 18 17 16 p--- Ported of --*1 ________Exposure____________________________ Time, Weeks xper'ment No. 1 .0 Micrograms of Aroolor 121+2/llter Figure 4 MGNS 088873 W e ig h t, Grams 8.6 Micrograms of Aroclor 121+2/lltor Figure 5 MONS 088874 b.i Mlcrograma of AroeXor X2l*2/llter Flgur* 6 MONS oaaa?5 Tims, Weska xpe-lmni t No.l 8.6 Mlerograms of Aroclor 12l*2/llter Figure 7 HONS 088876 W e ig h t L^oe.rlrcent. No, 1 8.6 Micrograms of Aroclor 12l4.2/llter Figure 9 MUNS 088878 R O lg h t, D ram s Time, Weeks Experiment No. 2 fc.B Mlcrogrnms of Arcelor 12l42/lltor Figure 10 MONS 080679 Weighty Grama !;,xi>nrtm. tit. No. 2 6.8 Mlorograma of Aroclor 12lj2/liter Figure 11 mons oaaaao / Figure 12 mgns oaaaai < TT h t- i: i Time, Weeks .jxBerimant No. 2 6.8 Mlerogrnraa of Aroolor 121+2/llter Figure 13 mons 08888^ W e ig h t, G m ras Mice Bk4l to B250 end B376, B464 ------------------------------------Period of------------------------J 33 "T Exposure -------------------1 32 . // 1 ,lk 31 33 29 28 l ' ll A J L / n \A i , Kl Vr V KM f K ' 3 ' 27 /v r 1 26 \ . _ .................... __ i______A i. AK { 25 i 24 23 i 7A . K' i A________ l4j_________ ___________ i. i./ 22 21 f i / 20 j \l 'v At !\ A| |! AM\.| ,, . ! 7I A! 19 yv1 18 17 16 15 14 13 Time, Weeks J'.nci'lir.erit No. 2 . fe.B Micrograms of Aroclor 1242/llter Figure l4 mons uaaaaj r 1 250 '.fe lg h t, Grama Time, Weeks ..:'n.'rlicr.t Ho. 2 6.8 Micrograms of Aroclor 12142/liter Figure 15 HUNS Quoad* i 1t I W e ig h t, Grama Time, V/eeka "i'xporlraont. No, 2 6.U Micrograms of Aroclor 12U2/lltor Figure 16 MONS 068885 Rata L526 to L535 and L836 320 310 300 290 200 2?0 260 250 240 230 220 210 200 190 100 Tima, basics '.xr.av'jaont No. 2 6.0 Mlcragrama of Aroolor 1242/llter Ptgura 17 MONS uaaaua 10.00 Rabblta I37O to 1373 and 1964 ! S.??T0| 1*200 1*100 1*000 . h T\/ . ______________ J J | 3900 :z t---------------------------------^ t- i 3000 ---------------------------- _i 3700 J3600 1--------------------------- -------------- -- 3*00 31+00 3300 H----------------------- /-------- Period of------- i Exposure J- 1 ----------------------------1------------ ---------------- --------------------------------------- 1_----------- 3aoo 3100 3000 f- 700 W e ig h t, G rans 060637 V e lg h tf G ra o i ^oo 4400 Rabbits 1370 to 1373 and 1964 4300 4200 .... .A* rr^1 'T---Hi 4100 4000 3900 3800 3700 3600 3500 3400 3300 3200 3100 3000 2900 2800 2700 2600 2500 2400 ^ /l f V\/ -K /A j /! / r .J J -i / -I/' 11 I--I (/ 1'^', / , /V1 p 7} fr J. / U f' v / F / 11 /, r ( P / ^ i Tims, Weeks SxperJmont No. 2 6.8 Nllcrogrems of Aroolor 1242/llter Figure 19 MUNS Ouodad 1 W e ig h t, G rans Experiment, No. 5-4 Mlcrogrnms Tine, Weeks 1 of Aroclor 1254/llter Figure 20 MOMS 0datid9 W e ig h t , Ox*runs xperlineiit No. 1 Time, Weeks 5 -U Mtorogrnme of Aroclor 1254/11ter Figure 21 MONS 088890 W e ig h t* O rn o is Time, Weeks .'.perlnaub No. 1 5.4 Mlorogrems of Aroclor 125^/llter Figure ZZ hons oaaa9i Gulnon Pigs B319 to B324 nnd B4l5 rod B4l8 800 1_> 700 r------- 6oo I % 400 t -j'"' to _y- 300 .1 ^tl^li-++' +|"J ' 1 / s. 200 ------------ 1___ Period of --------------- ;-------------Exposure xporircent No. 1 Time, Weeks 5.4 Mlorogrsms of Aroclor 1254/llfcur Figure 23 moms oaoa'jz Tlmo, Weeks "xperinieril 114. 1 5.4 Microgrnme of Aroclor 125U/HteI* Figure 24 MUNS 088893 250 A, 225 s/ " 200 250 t \ 1I Rats L299 and L5I7 to L520 "'1 T1 / \\~~ 1 Kllle 3 225 M--i-'t----r'pf--f'L'517 200 225 200 --("'-(--'A --1 * i~ ' Killed 300 2?5 . _____________________ 1.-4_____ _ 250 444-^i-A'j , ltsi? -/T^+^F-^ 1 1 ` Killed 22S ' 200 275 250 225 / T 200 ' '' ' Exposure Killed Time, Weeks zporlaorit No. 1 5.4 Micrograms of Aroclor 125h/llter Figure 25 MUNS U0da94 250 iRta.k521..to L525 225 200 275 250 L521 Klllud 22S -| 20? Klllud g ^53 *2? 230 1:.!'^Vh~h & 2to 175 S 300 * 275 250 225 250 225 200 7^ 175 Period oExpo3ure L525 Killed 1- Time, YYeeka :..rpcrlmont No. 1 5.1+ Mlcrogrnras of Aroclor 125lj/U-ter Figure 26 HONS 088895 W e ig h t, Grans 5.4 Mlcrogrnms of Aroolor 125i(/llfc0r Figure 27 mons oaaa96 W e ig h t, Groms 1*200 1*100 1*000 3900 3800 3700 3600 35=0 31*00 3300 3200 3100 3000 2900 2800 2700 2600 2300 ' ixperiment No. 51+ Micrograms Time, Weeks 1 of Aroclor 125l*/llter Figure 28 mons oaaa97 W e ig h t, Grama 21+00 2300 2200 2100 2000 1900 1800 1700 1600 1500 li+00 1300 2900 2800 27OO 2600 2300 21+00 3000 2900 2800 2700 2600 2900 2600 2700 2600 25OO 21+00 2300 2200 2100 Rabbits 11+53, 1587,1718, and 1887 \l 1 K ... ' \ \ 1 I! 1 s ^ 1 Died . ........ ; '1 1. mfl7 1 Died ..-A /' \ 1718 vDled _ y /n ( / , Jn~r\ 1 11 1 i-rn -1887 Killed __l S ----- ............ - ----------- Period of Exposure -------- -* irperimont Ho. 5.1+ Micrograms Time, Weeks 1 of Aroclor 125l+/llter Figure 29 MOMS 088898 W e ig h t, Grams UH03 4100 4000 3900 3803 3700 3600 3300 3403 3300 3200 3100 3000 2900 2800 2700 2600 2300 2400 2300 2200 2100 2000 1900 1800 1700 1600 Rabbits 1362 to I3b4 and 1453, 1587 1718. end 1887 L , \/ / dr V. ,/fN yi-i--- V LZ zv1 ' / / ,/t / --- / l/ T U' /J si / v_!j s( k-t /' r ^ LJi 3 _1 _ L_ j _ _ _ _ _ _ _ _ _ _ _ _ _ \ (Jr "Period of Exposure . Tima, Weeks Lxrarlrr.ant No.l 5.4 Mlorogrnna of Aroolor 1254/Hter Figure 30 MQNS 088899 W e ig h t, Grams Time, Weeks Control Subjected to Conditioned Air; for nxperiment Bo. 2 Aroclor 121+2 and llxpariraent Bo. 1 Aroclor 12^1+ figure 31 MQNS 068900 W e ig h t, Grans Time, Weeks Controls Subjected to Conditioned Experiment No. 2 Aroclor 1242 and xperlment No. 1 Aroclor 1254 Figure 52 Air; for MONS 088901 8oo Time, Weeks Controls Subjected to Conditioned Air; Experiment No. 2 Aroclor 121+2 snd '.'xperlment No. 1 Aroclor 12^1+ Figure JJ for MUNS 006901 Time, Weeks Controls Subjected to Conditioned Air; for Ljcperirer.fc No. 2 Arcelor 1242 and Hxperlxoenfc No. 1 Aroolor 1254 Figure 34 MOMS Udd<90J 30 29 28 27 26 25 24 23 * 22 21 20 19 18 Time, Weeks Confcvvla Subjeoted to Conditioned Alr;for Experiment No. 2 Aroclor 121+2 and Ixpeiiment-No. 1 Aroolor I25I4. Figure 35 MtlNS 066904 < e lg h t, Grams 2i3 225 200 250 v225 7 200 Rats L507 to L512 -t-H^ 'l~' 1 1 r1 1------------------- r L537 Killed 'Vrr -~4--t--1-- ___(----f-' L$D8 LKllled 250 K225 -4'~h 200 l\ '1 `'' - L509 Killed 275 y 250 ___________ t.i, 225 LJ-y __________ -4-- "1 --1-----1- L510 JCllled 200 2^0 225 200 V1 Ap+Y 175 1 \ 150 \ LS11 Died 275 250 ^ v-1225 . 1 1 u-'K. ^_K=t=H-+^ i512 ry Killed 200 175 Exposure Time, Weeks Controls Subjeoted to Conditioned Air; for Oxi-h rinent No. 2 Aroclor 12l;2 and Experiment No. 1 Aroclor 1254 Figure 36 088905 1 Hats L513 to L516 and L748 275 250 225 v 1-- I 200 ^TT 250 L225 200 175 275 i 250 6 225 J 200 250 M"H'"' ' L513 Killed .'J:g514 Killed 1515 Killed 5 225 200 175 275 250 225 NtPl^t 200 Period of Exposure Time, Weeks Controls Subjected to Conditioned Air; Experiment No. 2 Aroolor 1242 and Experiment No. 1 Aroclor 1254 Figure 37 for MONS oau90b Tima, Weeks Controls rtubjosted.to Conditioned Air; for "r.neri'nent No. 2 Aroolor 12li2 and ' x^eriment "No. 1 Aroolor 125i| Figure 38 MGNS 060907 W e ig h t, frame Figure 39 moms oaasoo W eight, Grams Rabbits 1368, 1369, and 1975 . -r- ^ `"'"UfeB ' "Killed 4000 3800 j-r. V' 1 y. 1+600 ..... .... ........... . ____ .-V. r TTF Jn' 1+1+00 jL_____________Ll 1+200 ^A rp t ^_____\______ i+000 . -f 1 3600 3400 3200 3000 2800 2600 5800 3600 ./ I iA l _____ 1369 Died \i31+00 j r 5200 -------------- 3000 2800 If A \ " P,srlofi of SOKpoaure ^ 1975 ' iyxX-Lwu. 3/ 2600 21+00 / / < 2200 Time, Weeks Controls .subjected to Conditioned Air; ^or r\xporiment No, 2 Aroclor 121+2 and Uxnerlm?nL;No, 1 Aroclor 1284 Figure 40 MUNS 088909 W eight, Grams 'Time, Weeks _Controls Subjected.to.Condi Cloned Air; for 'xp<;rlment No. 2 Aroolor 121+2 and 'xnarlment No. 1 Aroolor I25I+ figure 1+1 MOMS 088910