Document NEx4MLNzngxKRowRZQrB10LKR
AR226-3091
DuPontHL-1998-01441
HASKELL LABORATORY FOR TOXICOLOGY AND INDUSTRIAL MEDICINE
H-22762: STATIC, ACUTE 96-HOUR SCREENING TEST TO FATHEAD MINNOWS, Pimephales promelas
PURPOSE: This is a rangefinding study designed to provide an estimate of aquatic toxicity using fixed
test concentrations and data measurements at predetermined intervals.
GENERAL INFORMATION
WORK REQUEST NUMBER:(| HASKELL NUMBER:.:
TEST CODE; MATERIAL TESTED; SYNONYMS/CODES;
SPONSOR:
NOTEBOOK ED;)
SUBMITTER'S NOTEBOOK NUMBER:
COMPOSITION: CONTAMINANTS:
CAS REGISTRY: PURITY:
TEST CONDITIONS
Nominal concentrations tested: 0,0.5, 1.0,50, 500, and 5000 mg/L.
Nominal Concentration
(mg/L)
0
0.5 1.0 50 500 5000
Test Initiation (O hours)
Dissolved
Oxygen
(mg/L)
PH
8.6
8.0
8.7
7.9
8.7
7.8
8.7
7.7
8.7
7.6
8.6
7.3
Test Completion (96 hours)
Dissolved
Oxygen
(mg/L)
PH
7.4
7.6
7.7
7.5
7.6
7.5
7.6
7.4
8.5"
6.8*
8.53
6.38
a Measurement was taken 24 hours after exposure started due to total mortality.
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not contain TSCA CBJ
DuPontHL-1998-01441
H-22762: STATIC, ACUTE 96-HOUR SCREENING TEST TO FATHEAD MINNOWS, Pimephales promelas, CONTINUED
RESULTS
Based on visual observations, the water control solution and the 0.5,1.0, and 50 mg/L test solutions were clear and had no color. The 500 and 5000 mg/L test solutions were cloudy with a surface film and undissolved test material present. At approximately 24 hours, the 500 and 5000 mg/L test solutions were clear and had undissolved test material stuck to the sides and bottom of the test vessels, and a surface film. The remaining test solutions and
control remained unchanged throughout the study. Mortalities were 0,0,0,0,100, and 100 % in the 0,0.5, 1.0,50,
500, and 5000 mg/L test concentrations, respectively. Twenty percent of the fish in the 50 mg/L test solution exhibited a partial loss of equilibrium approximately 24 hours after exposure started. At approximately 48 hours after exposure started, all fish in the 50 mg/L test solution appeared normal. The 96-hour LC5o, based on nominal concentrations and mortality, was between 50 and 500 mg/L. All water quality parameters were within acceptable
limits.
CONCLUSIONS
H-22762 exhibited slight toxicity in a 96-hour, unaerated, static acute test using fathead minnows, Pimephales promelas.
WORK BY:
^ajllfLUL.^^fe^hiliJL
Laurie D. Bouchelle Toxicology Associate
APPROVED BY:
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(\
M^.ho^ Paul W. Grube, B.A.
Study Director
DISTRIBUTION:
K.Dastur(l) S. Mileti (1)
E. Holloway (1) B. Brock (1) H. Trochimowicz (1) S. Cattie (1)
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Month/Day/Year
-W(f W
ay/Year
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