Document NEoZmdnEy28jYnKOaQmadOk9R

O.W.H. CASEY Slh tel,*-, mo. - SfL ^ wS *>**TT' WdC/rao A.f. RECAN. A maotlno eenecmino oolychlortoiohonyle in connection 1th CLC Analytical fathods end t'lldlife oea held nt the Twstnll Leboretorlee of fhell ftaoaareh Limited at Sittlnobourna, Kant on 20th rontanber 1071. The noetino me orranoad hv tha ninietry of Agriculture. Fioherlee and food* aid follow'd a almiler aaatlnq hald in 1969* Approximately fotty nooola attended. aelnly fro* various bnviehea of tha riniatry and ralatod ftataarch Aaaoclatlona. favrral unlueraltlae aara rapraanntad. Industrial reproeentatlen aaa 11*1 tad to 'JUlevtr, Shall anil Consanto* Tha procaadinqa uara cooduetad infernally. although nresentntiono on vnriou* tcplca sere ado in brlaf lactura far*. Soaa notaa ora oraaaotad. SjJ]Mljy_[flBjof^^ gave a brlaf aeeount of tha oravioua nesting ahlch had boon hald to dlaeuaa varloua aspects if PC9 determination. Tha intoraat had arisen froa sorb eartlad out on dataralnatlon of varloua ehlorlnatad naatelda raaldua* In anlaal atd nor1cultural samples share tha PCB'a flrat apoaarad a* ifbnaai contaminating paaka. A. Hichardaan rrhalll diaeucaad aoaa aapaoto of 0I>T and PC (I analysis. Ha aontionad `Jura | Can*0*1 oho had avolvad a taehnlcu* of catalytlo * raduetlon/hydropanatlon eonvartinq PCS'* to dlcyclohaayl typo hydrocarbon* for flD dataralnatlon. This aaa generally thouoht to ba of llttla uaa if tha anolvot had oecaaa to EC detection. Claen-tp techniques aara briefly mentioned* Tallinp a SlBoona fwllavorl outlined their aathoda anphaalrlng on oxirr.tlvo doan-m orocaaura. Total reeovorlao rare good, in tho 90-95', rntgo alth tha naln loaooa assorting durinq aelvont rofnoval* Their aaln raaaereh-tyoa development ana a ohlft to hlgh-reeoiution [eg* conlllary] chroma toqwphy nith uaa of coupled ohyaleal methods s.q. or vs in order to eatablieh panic identity* Thia ahoesd that aoa ordinary GC peaks aara actually aultiplato. a Cleno lo oroeaduraa sera mentioned. atrooalnq thoir naod share other raaldua nesbs overlay PC 9 paaka. A nee r.C o tationary pheaa ooly-nphenovylene had been triad and aoa on* of tha foe phases found to sluta OOC j^a ehlorlnatad q^Ueldo') In on area fra* froa lerqn CN peak** The phono hod a good upper temperature Unit of about eork. /cant, HONS 212313 2 r - -tt l~r r| r'n-.nur-Snnl. n.A.L. brlofly outlined tha *tCL position oe far op' J.7: jytlccil n--thO(J8 worn coneornod rantlonlnq that tho C "methodology pnekoge" had baan nvellobls. The present research Investigations into hlghreeolutlon CC Uh other physical method* bainq used to aatabllah paak ldantlty aara also indicated, Tto writer, oeked to cunrant briafly from a plant producinq Aroclora, outlined the squlpmont evellnble at Neaport and aantionad ot* aonltorlnq of effluent dioeharge td advisory earvlee ta cuatoaara, or any other Interseted parties, Profr''`t'nr van Crndnrnn f Mtracht, Holland*! dooerlbed aora of the sork on Pen's utr-cht. Thrne eomrarcial chlorinated diphenyls rare bainq looked et In one pyrtlnulcr investigation, These were, Aroelor 12G0, Clophan [of Carmen origin? Qeyer[ unn uhennchlor | franeh oriqin - f Prodalecl, both of the latter being In the we range aa 1260. theee three appeared to be of differont tonicity lavala elth Aroelor 12G0 bainq the least herafui from the studios elth contrallod diet feedlnq to reto. Analytical lnvastiostion showed that the Clophan and Phenechlor contained coapounda of the followlnq types) tetre chlor dibonnfttfan [panto] Tatra ehlor dlbenxo diexadisna [pants] ahich fron other work are knean to ba quite tesiei in particular they can aet ip . akin condition# of tha chlor*eene typa. Peeeurenent rad idantlflration wrrn mada by a combination of qoa chrawatopranhy rad naoo apaetrooatry, Arcelor 1200 eaa found "i have non# of thaaa coapounda, Clophan could hove ip to 5 ppn and PhanochJor up to 20 ppe, noldwn [fishery ftnsworch, Pltlochryl, spoke about possible aortas of entry of pCB'a Into tin- r-ut-cl environment |_nooclelly tho narlno environment]. One apoorent source that they hod uncovered was fnm the dumping of eeeeqe eludgeo, Gcweqe ferns in ths Clasooa area periodically dunped elbdgea sell dean tha Clyds rad sons, but not oil of those ferae had olodges elth PCrt contents qivlnq a total dm of about 1 ton of PCO por year, Slellor cheeks in the London ares had shown tha sans otsta of offolrn# Tha Bristol raqlra, hosawar, had not ohown ray northwhllo dine rota. Tty speaker tendered whether land dtnpa olao praaantad a possible route via tha tnimel feedlnq chain lo* for wall eraaturna to pick ue PCft'a In their diet, onding us pnrhms with tho largo raimolo ouch as birds rad various marine animals* Me rantlornJ that aa yet no PCB'o hod boon found in frwoh ratar fish /cant, HONS 212314 s rfnnitlio rr^lTw rnllnrwl. Speaklno on entyae Induction affects of PC5*e Tincrlbtul esrier linen to In progress on rate whore rather large [25mo/kg bodyweight] injrrtlons of Aroelor 1?42 wen given* In tooto looting 141 to 20 doyo no ill* offsets oare oboerved In thot tho rats did not die and remained visibly healthy. Pn oxomlnntlon shoaad tho ejected lncroooa In fatty tioouo nrotxtd tho livor which la not poeulior to PC8'e, but lo eouood by many other compounds no aall. Vorinuo In vivo and In vitro tooto wars described to demonstrate thot the r>C9 offset eocnod to bo eeuolng at incnooed probation of various enzymes. f>nnral Ptncurlon* Ccmldersble general diccueelon took plan and 0one polnto era noted boloat 1. The nonoanto attitude to the PC* auaotion was seraiy welcomed in that tho Company woo taking port In tho ptenont dineuselone. In thot It node Information on methods roudily ivi-Usbls aid provided snaploo of Aroclors, and woe plainly worn to bo e-operative* 2* nny epcokere doprocatod the Press 0recantation on PCB'e which tended to linoly thot this seo tho major reeaon for toxic 'fncta particularly on aeo birds end other eorine minala. Co one Booste r oucclntly put it "why bine PC 1*0?*. It aao hoped thot the prenent accent on PCI'e wee not drawing off imecoeoerlly o lorge amount of technical effort. taro the mCL peoplo could only point out that Aroclors hod boon wads in thlo eointry for 2fl years. nrt in tho U.S. for obout 2t\ yeoro before that and that one sight hove expected toxic affects to have ohon m earlier* 3* Th* Bristol Channel area see auctioned oo having bean surveyed for evidence of PC3*e and ooae surprise eae felt et vlrtuelly none being fowd. although the recnert Plant aao In that ores* We strasaad that our corks effluent aao monitored and the PCS discharge was at a very loa figure* [no figure toe mentioned, not see referanas mads to estuarine mud examination!. 4. ana rpmtker gueotluned the normal variation in chlorine content of an Aroeler. The aneeer of about 0.5* on the nominal value aith no effeet on the interpretation of a PCS chromatograeh aao aatlofactory. This qLmotlon seemed to indicate that many people working in this field did not heva nil tho taehnieol information available to them. The lnoroaslon wee formed thot aoma peoplo without a chemical background felt that s.g. Aroelor 1242 non e distinct chemical entity, different from e.o. Aroelor 125J. ond did not fully appreciate that bath asre mixture a of similar ehloro compoinda in differing ratios. 5. rant!on aao made of the feet that some toxicity work sac being dona on Aroelor 1750, conn on 1242 nnd 00 on. Thlo lad to questions on the Quantities pto'.'ucnd of tho various grades and also on the uac^ottem by area and parhaos by industry. hono of those oueetiano see onewered direetly. but it ana pointed out thnt eert&ln lnfomntlon might bo made available to workers in the field if a written roqurjut aoe prosontod. . ' /corvt... HONS 212315 4 Thin might anahla interest to be concentrated on non* Ptff'a more aidely uc*H than othnre* It <moo further pointed out that WC mi not the only producer of PC9'e end thua North Sob pollution might bo arising not from me motorial* In thlo raspoct oooo of Profeeaot von Genderen'e remarks coro qulta relevant* 6, The trend to hinh-reeolution CC and lndentlfleetlon of peaks prented several poopla to point out that tht.re could bo considerable dlfferenerr In toxicity botwnon the vnrlouo components of a PC ft. Altheuqh the else of tha teak of Identifying all tho peaks lfl o chromatogram wao loroo vnour,..* thot of attainting toxicity atudios on individual cooponanta aaa vary much larger* Stnuarv* The mooting served to hoop heapert in touch eith poaelbla analytical dovslopiaanta in tha PCB field* and also outlined tha aider complicatione* It visa also fairly obvious that although tha Company hee made much Information availobia, aa might atill have more to do in the nducetivo line even ir only to ensure that people do not aake erroneous eaeumptiene obout PCB*o. MOMS 212316