Document N2wx4v62Dr06zvKn8dVJXvJd8

Copies to: E. I. du Font de Hemours and Company Haskell Laboratory for Toxicology and Industrial Medicine HASKELL LABORATORY REPORT NO. 56-78 MR Y.O. Material Tested Haskell No. 11,890 Sample Ready for Testing 12/2/77 Other Codes Material Textile Fiber Seaford. Dela 48-HOUR LC502 TO DAPHNIA MAGNA Procedure: The test material, in original form, was diluted with test water to yield the desired conc 400 ml final volume. After mixing, each concentration was split into two equal volumes and introduced 250 ml glass vessels. Identical jars containing only test water were designated as controls. A nomin daphnids (Daphnia magna; less than 24 hours old) was placed into each vessel. Mortality counts and ob made at 1, 3, 24 and 48 hours after initiating exposure. No aeration or food was provided during the was maintained at 18 0.2C. Dissolved oxygen, pH and conductivity measurements were taken on the co exposure solutions prior to test initiation. Calculation of the 48-hour LC50 and confidence limits va (Finney, 1971).3 RESULTS: Definitive Test Test Concentration w 24 21 18 15.5 10.0 7.5 Control Observed Replicate 100 100 40 10 0 0 0 Mortality (.?,) A Replicate B 100 100 50 10 0 0 0 Initial Water Quality Supply 24% Water Exposure Dissolved Oxygen (ppn): 8.6 8.7 pH: 7.4 9.0 Conductivity (Umhos): 370 400 Observations No distinct sublethal effe 48-hour LC50: 17.8% 95% Confidence Limits: 1 Dose-Response Slope: 25.6 95% Confidence Liraits: 1 Company Sanitized. D AR226-2867 RESULTS Con^t. 001189) The high initial pH of the 24% concentration approaches values which may potentially cause or contribut toxicological response. Similar observations on bluegill sunfish bioassays pronpted supplementary test and unneutralized test materials Those studies indicate that the observed pH did not con acute toxicity of the test mater i'-ointhe bluegill bioassay and suggest that the observed pH did not ute to the acute toxicity of the test material in the daphnid bioassay. 5n^^^y: lJ|^^^HH^^^^^m^J^^^H||^IIHIIfflexhibits very low acute toxicity to Daphnia maena unaeraced test conditions during a Ao-hour exposure. Its 48-hour LCSO is 17.8% with 957. confidence li 18.6Z. The slope of the dose-response curve is 25.6 with 95% confid<;nce limits of 15.6 and 35.6. 2 LC50 = The concentration which is lethal to 502 of a population of test organisms during the specif 3 Finney, D. J., Probit Analysis, 3rd ed., 1971. Cambridge University Press. XCG/cgs Date Issued: 2/10/7b Report Ko. 56-78 Report by; "7'' c1 <? / / ^z-r^^,^ - ./-r-z- ^^7- Kany C. Goodman, Tec Approved 'by:= ^ i . ^tuI^llO^ltou^- '^^__ '^hillip V. Schneid ;hief. Aquatic To Company Sanitized. 2 - jijijb *>3 i-^ r iil.^^'.b JCiiLi- ol-' uLiliL, Iw ftJ it : Ti<llHS;-i..i'.i:i'. TO LuJ(10) Ii,FoI LATA \^M. 2l;.GCOO 2'l.OCOO 21.0COC 21.COOO 1E.OCOO IS.0000 Ti.^OOQ 15.500C 10.0000 10.00CO 7.5CCO 7.5000 u0t Ubae 1.3302 1.3S02 1.3222 1.3'22 1.2553 1.2553 1.1903 1.19'03 1.0000 1.0000 O.teTSI 0.57i;1 (::XPl.)Si::l; 10. 10. ; 10. i 10. ; 10. 10. 1C. 10. ' 10. ; 1C. ' 10. 10. ii SESPQ:iDIllG 10 10 1C 10 X 6 1 1 C 0 0 0 KATb 0.9595 0.9999 0.999') 0.9999 O.ttOOC- 0.5000 n.iocc 0.10.0 ; C.CCC1 C.OCC1 O.C001 O.OC31 pni-i.lT f.7^f1 e',7101 i.Tl^l '..71-;1 I.?'':'') H.W! 3.71:'1 3.7131 1.??; I.S^C-) l.?'-"^ 1.2>-^^ C'Jl.TivuL aAi'.PLL aiZf. a 20. CUi.iiiL'L l^i,a^Ui;rii;.& s 0. at-Oi.TA.<LJU3 HE;Fl;n^ hTl-' : i =0.0 0000 H&TEHOGLi^m ?ACTOR s I.Ulilil;!"! 01-' t-OI;;':;! = DEGi'iEEa 01'' FH2ECO;; s CEVIATii s G s 1.0000 1^ 11? S.S'tsOO li.1531 (^" iiUilESR OF CYCLF3 s AVG X s AVG Y = AVC V = SLOPE = JUTbiiGEFT s Cr;I S.SOABEL- s 'I1 .2510 !i.0136 :?'..t|1')')1199 2! >.62CC -2' '.03SS: 2.1S39 LL = 15.5951 ut = 3S .GUGS POIxT ps .01 P= .05 Fs .10 l'= .20 Vs .'-.C ts .^0 Fs .90 1-= .OS 1-= .W DOSE T!.41'1 15.3563 15.6660 10.5059 17.<;.M."; TJ.irOli, ly.OTt-l 20,65-31 21.9't21? i 95i CO:iF'IDEi<CE i.Q\:'c.H 12.5109 IB.TS^I TI.^ISZ 15.^130 17.''7';1 ^..iHQ9 i^.c.'."9 19.'^Z ?0.'31'J9 LIilITS UPPER 15.15'iC 16.1955 16.6275 17.191^ r'.cr.;T <;0.53.->- r^.w.^ i!2.W-' 2'j.2551 LIi;llLIHC<0^LIl.r. FOR i-'-ll?'.';) t-i.u'i uF THI; liAy.Ii-.Ul. i Company Sanitized. Does not contain TSC""A' -C"BlaI ^.^a^,^^^^ 1-t.^t Uf T:^ i./.;-:Ii;U;. Lji.lLUPuOLii Ui'^ PiJi' Tin. ..A:-.i;;ui. i-ILi-LJt'^iU E.STIt'ATp;; nit!:! SLUt-li; 25.&2C; lini.i'.CKr.'s -27.^3-;5 ; tl-Ot.'t'A.^uUsi nt.JI-'U;i5E iiATasD. 00000 7.0 * o.'j * o.c + 0.7 + (l.U + u.5 + 6.1 * C.3 * U.2 u.1 + C.O + 5.'; * 5.& + 5.7 + 5.6 + C.5 + '>.')+ 5.3 * F 5.2 + R 5.1 + 0 5.0 + t.9 + I 21.& T 1.7 + 1.6 + .5 + l.'l + k.3 + 4.2 + 1.1 + f.O + 3.5 + . 3.6 * 3.7 * 3.6 + 3.5 * 3.1 * 3.3 * 3.2 * 3.1* 3.0 * : ' ; ; , , ; ; + + ; + + + * . i + * + ; *^ ' ! * ; + 5 * . . 4 . + ; + ' ' i . + + + : ; ; , ; . ; ' + C.75 * i <i * O.y,'u l.CCil ; I.IS1! LSG SUS& + 1.2C7 4 I.Z^C fciiL JF CXECUTIOii Cr. Il.L: C.C3 Ll.IT , ELAF;jE& TI;'?.: ; fc;C.55 Company Sanitized. Does not contain TSCA CP5