Document N2wVb1XgE90j0JY4nOKad4kmb
AR 226 _ 088I
ACUTE TOXICITY TO FISH (FATHEAD MINNOW)
IEST~ S ~U ~ BSTANC0 G 0E 00 Identity: A mixture containing perfluorooctanesulfonate, which may also be
referred to as PFOS, FC-95, or as a component of FC-787 or FC787X. (1-Octanesulfonic acid) (CAS # 2795-39-3).
Remarks: The 3M production lot number was 101. The test sample is FC787. Current information indicates it is a mixture of 0.73% PFOS, 10% diethylene glycol monobutyl ether, 79.74% water, 5% ethylene glycol, 1.47% propane Sultone foamer, 0.73% sodium octyl sulfate, 0.73% sodium decyl sulfate, 0.75% xanthan gum, 0.75% magnesium sulfate, 0.10% N-(3chloroallyl)hexaminium chloride, and 0.05% benzotriazole.
The following summary applies to a mixture with incompletely characterized concentrations of impurities. Data may not accurately reflect the toxicity of the fluorochemical component of the test`sample.
ME0 THOD:0000000
Method: `OECD Method 203 and U.S. EPA Method EG-9
Type: Acute Flow-through
angen. GLP: No
Year completed: 1990 Species: Pimephales promelas Supplier: Commercial supplier.
Analytical monitoring: pH, temperature, conductivity, and dissolved
Exposure period: 96-hours
Statistical methods: The LCs values were calculated using a computer
program by Stephan, 1983.
Test fish age: Juveniles
Length and weight: Average length = 34 mm
-
Average weight = 0.32 g (wet)
Loading: 0.21 gl.
Pretreatment: None.
Test conditions:
Dilution water: Filtered natural well water
Dilution water chemistry:
Hardness: 180 mg/L as CaCOs
_
Conductivity: 1300 pmhos/cm
pH:
83
Lighting: Cool-white fluorescent lights at 18 pE/sec/m?. A daily photoperiod
of 16 hours light and 8 hours dark was maintained throughout the testing
period.
002643
pSrteopcakreadndbytedsitsssoollvuitnigo6n0p0rgepoafrtaetsitosnu:bsAtapnrcimeawriythst1o0ck00somluLtidoinluwtiaosn water.
sEoxlpuotsiounrsewciotnhicnentthreatpiroonposrwteiornealprdielputaerr.ed by serial dilution of the stock
FColnocwe-ntthrraotuigohnrsatdeo:sAivnegraragteeo:f
Continuous 6.7 complete
volume
changes
per
24
hours.
Stabilityofthe test Exposure vessels:
c2h0eLmigclaalsssaoqluuatriioans(:20Nxo4t0xn2ot5ecd.m)
containing
15
Loftest
Nsoulmutbieonrowiftrheaplwiactaetresd:ept2h of approx. 18 cm.
Number Number
of of
fish per replicate: 10 concentrations: five plus
a
blank
control
Water chemistry during the study:
pH range (0-96 hours):
78..08-88..35 ((c1o0n0t0romlge/xLpoesxuproes)ure) -
Temperature range (0-96 hours):
2222..43-2222.57
C C
(control exposure) (1000 mglL exposure)
Conduct1i3v0i0t-y1r5a0n0gep:m(h0o-s9/6chmou(rcson)trol exposure)
Dissol1v3e0d0o-xy15g0e0n prmahnoges/(c0m-9(61h0o0u0rsm)g:/L exposure)
8.08.7 mg/L (control exposure)
3.06.5 mg/L (1000 mg/L exposure)
R0 Esovs 000
Nominal concentrations: BK control, 140, 240, 400, 610, and 1000 mg/L. Element values: 96-hour LCso = >1000 mg/L
Element values based on nominal concentrations
SRuebmsatraknsc:eTReesmtianrgkwsafsielcdo.nTduhcetveadluoenstrheepmoirxtteudraepapslydetosctrhiatbemdixitnutrheeaTnedstnot ~~
the fluorochemical proportion alone.
conclusions
00000000
`The test sample 96-hour LCsoforfathead minnow was determined to be.
o>b1s0e0r0vemdg./L in both replicates. No mortality or sublethal effects were
_.
Submitter: 3M Paul, Minnesota,
C5o5m1p3a3ny,
Environmental
Laboratory,
P.O.
Box
33331,
St.
002644
tReesltiianbgi.litHyo:weKvieirm,istchhersaanmkipnlge2p.urTithyewsatsudnyotmepertopsertlhye
criteria for quality characterized and
the
study lacks analytical confirmation of the amount of fluorochemical proportion
in the solution.
R0 EFERE0 NCES 0000000 This study was conducted by EnviroSystems Division, Resource Analysts, IRnecq.,ueofstHanmupmtboenr, HN1H60a9t,th1e99r0e.quest of the 3M Company, St. Paul, MN, Lab
o0 mWE0 R 00000000000
Last changed: 6/26/00
002645
Study Title toFltohwe TFhartohuegahd AMicnuntoew,ToxPiicmietpyhaolfesFCp-r1o8m7elas
Authors. TRoibmeortthyL.J. BoWearrid
Study Completed Septenber 20, 1990
rforaing Labor:
ResouErncveiroAnSaylsytsetmss, DiIvnicsoiropnorated
-
OneP.L0a.fayBeotxte778Road
Hanpton, New Hampshire 03342
EPnavgiero1Syosfte1a7s Study Nuaber 9069-3
ResuscaAnasnc. Subs ofMLLFORE
002646
I. GOOD LABORATORY PRACTICE STATENENT
PractiTcheis Regsutluadtyionvsa.s conNdeuicttheedr thaeccoSrtduidnygDirteoctoOrECDnorGotohde spLoanbsoorratosrrey
Satvuadrye. of dissolved
Ataorntyyhgeencisrpccoounnnscosertnsatnrcraeetsniuoensstt'h,ataabeorwvaoetuilodnSOVvaafsfescanttoutrtaehteaipolinon.vteedgrTithtoeyaaaoifvnettrahsiiens
NrLoeanngdtodhmeivizaa`ottfiioonn"sfisohfexaiststeesdtouttsshpiaetdceisseitnghsenifricacannagnetnlosytpecbaeifffievecedtreidfinietdhtehebyvparltohitedoictroyalv.ofdatTtahhee
stuey.
=
FAuTfartonryaInfd Wsstudy Director
Zi Pa 4 Z TCoobnourtthor. Bott
9.20.3
Qiunanteic*.ToHxaigcao,lbotat 213-90
PAoqtuhatiWc1l.ToTxoFivocarololsnokbgiiilst 13.
aEagveiro3syostte1n7s Study Nusber 9063-3
Puce Aron.Skin AMRLFCEE
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I. QUALITY ASSURANCE stATERDIT
Subsitted by: PER.ea0sv.oiurroBcsoexysta7ee1n8isyaDtisv,isiTonncorporated oHnueaptLoanf,ayeNtevteBefnoptabire 03642 -
Gortitication: DFeaptoartwiptrheesnetaimttaeltdeedriail"nsMetthhioiddsesntraienfpdioerdtMatveeirrniealdtseh.re"ivseedTchtebiyontaessotfthovtdehsse
Lp$a0eb6ro5fr-oa3rtaoer"dayndinSttahen"sdcPacrroodrdduucOntpceeRreagtIiitsnhtgratPEirnoovnicreodAsuqyruseatsteinNcs.aTmoaxPlir.cootorcToehq)ey
A
aFtnoodvxailcsifkitiny,al t`eRs"ortbeeprovtrtasBvoislrpile,rbfeoanrdasrecdThiiavobetydhyaGteHiarmRdme.esouMrTacgheeersrA,envalPyedstatetsra
Tne. for at least 10 years.
QJMueLarleitayscthaechkaestdurraanfnoscrceriabceAcduudriatcfoyrrosna.nd tahQe1uaadlvaittaydavsteasrseutroavnetcrheiefairueedspiotrbsty
Vere pertorned on:
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12/10/30
1710/3
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TPaavgieco3ssoafte1n7s Study Number 3069-3
Waist Sabpeern 002648
111. mane or comrams
.
:
sxetton:
na
I Good Laboratory Pescticn statement _
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II. Quality Assurance Statement
mL tele of contents
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Tote of bles
[Ar--
[A --,
VII Nethods and materials
mr rea
3
.
s
y
` ,
,
u
4
IX. References
1"
Can
Appendix A. ater Quality Data fron Toxicity Test
15
HE EaviroSysteas Study Nusber 9063-3
SEAMS5 dati nor
002649
i
Iv. Dox or Tasues
"
ace
BN
Tabled. wsCaahtmeeparlieefnloorfchtnaaortxauicrcatiletryivzetaledtsitovnateorf uaserdeprsesgednitlauttiivoen
s
Table 2. Survival data from toxicity test
1
"
Table 3. Mteodxiiacnitylettheaslt concentrations (LC308) from
1
Table A.1. oCxoyngdeuncticvointcye,ntrpai,tiotnemmpeearastuurreed,duarnidngdistsooxlicvietdy 1
"
teat
PEaagveiro5syosfte1n7s Study Naber 9063-3
itis
_
002650
V. SUMMARY
uprnodmeerlasTf,hleowisactuhdtreeosucgrhtiobcxeoidncdiittiyinonosfthifsoFrC-7f38Mi7naClomtpoarenpytohrefto.rfat96hTehhaoedurtmseisntndouwwr,aisngPciAomunegdpuuhscattleeds3 KBtooawmaplt7so,kni,,199N0eR,owberHattampsBthohieerrieE,.nviarnodSItysTtwieammsosthDycivoinsWdiauorcdntedofaccRboyersdoiuJnregcaenneAtnoalMNytashgteasz,a,prIonctP.oectoeilrn developed for EnviroSystems Study Number 9069-3.
concentrTahteiontsestofwastestperfsourbnsetdanceundearnd
flow throlgh a dilution
conditions with water control
five at a
temperature of water collected
23 at
+ 2C. Hampton,
NTehwediRalmuptsihoinrew.ateArerawatsionfiwlatserendotneatmuprlaolyedweltlo
cmoanicnetnatirnatidoinsssolovfed
oxygen FC-787
wecroen:cent0ratmigo/nLs
above 80% (control),
saturation. Nominal 140 mg/l, 240 mg/L,
400 all
camgl/cLu,lati6o1n0s.mg/L,
and
1,000
mg/L.
Nominal concentrations were used for
Fish acclimated
used under
in the test were test conditions
fpourrch14asedadysf.romAfatecromm96erchioaulrs soufppleixeprosuarned
wtehieghtcontr(obllotfteidsh
had an dry)
aofvera0g.e32 togt,al
length of resulting
3.4 in
cm a
and an average loading rate
wet of
oafpprthoeximstautdeyl.y 0.21 g/L. All fish were in good condition at the beginning
concentrTahteiontse.st
substance Exposure
was of
fantohnetaodxicmintnoowsfathteoad theminntoewsst
at tested substance
resulted in a 96 hour LC50 of > 1,000 mg/L FC-787.
PEanvgiero6Syosfte1a7s Study Number 9069-3
ResourceAnalysts.Inc. Subsidiaryof MLLPORE 002651
vi. oimopuctron otbhejectfiavTtehhiesadof smtiutndhnyeor,vstausadyfrsepsovhansusaotrteeorddsfbitysehr3.ainCeoTmhpetahneTya,pAocruSttt.ecotPnaotuxalii,cnist'KyimsnoefocstoiFtoCan-.s787tThhatero dtoefhsecwr`taihtbeeeriqatuvhieelsittimgyeatthdioaodtnsa.caonTldhleeacsttreeedrpiodsrtulrsianelgasopltohceyoendttaeisitna.sthaen satpupdeyn,dix4ndthattheprreesssualttss
VIL KETHODS AND MATERIALS
TEST suBsTANCE:
BnviroSyFsCt-eTn8s7 bottle that
waosn (EMnlvaaiybreolsl1ye5s,dtenvsi1t99h0.SihaemplfICeolvlaoNswuimncbgeorntianif2no0er69dm5a)tiinonwa:as1 "gPadele-l1lo6in1vebproletadst1i0tc1o
sasurfa"PcCtant7s81/,butyLlot Paul, Wimesota.
Pc1ra0ir1,obrta(lt1,o12u0s54e-71)0t%-h.9e0"tFesCat-n7d8m7a&tew1rasiistlseurvpapslpilesadtsotribecydb3ointCtoltmehpealndayba,ekllSeta.di
aoonn'inteampoefrat1u0rey.ears.A reserve sample vill be archived at Bavirosystems for
DILUTION WATER:
testingVateyras Huspabire. |
usceodllecftoerd Vater was
acafcrdlojaiumsatwteeidlolnstoofat'tsehstaEravdoinrcegosasnsyissotmfess1s8a0ndinngfo/rL
Raalslpttono,xicMietwy as CaCr and
cshteonriecdalinan5a0l0y-sgeilslonofpo&lyetrheyplreenseenttaastkisv,e Table 1.
vhsearmepleitofvasaateerrataerde.preRseesnutletds
oifn
TEST oRcANISN:
sTihoegylewoJrsueovuerncpieulrechaanfdsaetdhveeadrferosniidmaeonvtscifoimeemdmeprcliuoaysleidngsuapaspnliateeprsptropaornridgaatnaeicscatlsaixmoawnteoermdeicaftrobmetyh.ea
emEaervaeisruorsSeuydsitnetantasinetdfhaeicnilcio1tn0y0c8ludsfiiolorunti1oof4ntdvhaaeytset.roxuincdieCtroynttfrelosotlv. tfhPirrsohiuogrhwectrooendtietswtieioinngghs,edinfaiasnnhg
naldl the
tDghleeagysisnnwineagrquearoiffurse.s"theoDfurtiaenspgtp.araecnctDluisrsiiacntkginoentshsef,iashcicnvjluairrmisaetsin,oontapntderreiasotbdendorparfsiolroirtdiiteosseatsahtee
Wtdeeirssetsolfvioendditiaoatxiyocgnoemnmetrchceoinacletnetmrpfaietsrhiaotnfuoroevdas r(maEunnigvneitdoaSiynfsertdoemnsst 2o1lf.ot1%snbuosvtbeoer83.2T0.H5OnCLg,)/L.eanncdeFitoshhre
trice dafly except for the 48 hour period before the test.
EPnavgeiro7syosfte1n7s Study Number 9063-3
Ensen struc
002652
Teble 1. aChaetmuircaallwelclhawraatcetreriuszeadtioasn dioflutiaonrveaptreersenftoartitvoericistaymplteestof
Farancter
NUonaisturaofsent RTeipnoritting
WeTwasluureed
"
"n
B units
-
a3
conductivity
unhos/en
-
1300
:
Hardness: ftinniatli'al mg/h as Cacos -
210800
Opregsatsioccihdleosriae
wal
0.5
opregsatniocpihdoessphorus wal
5.0
w
bPioplhyecnhyllosrinated an
os
w
Wotes: 21.. WTDhe= novtaludeestectfeodr shpEo,ve cthoendurcetpiovrittiyn,g 1iaaaidt. hardness vere
dbeergiivaendiagfroofn tahe stuosxpilceityoftestdil(uhtairodnaewssatevrascoalllseoctdeedterataintehde
.
adte'rivtehde cfornocmlussainopnlesofof dtihleutitoenst)wataenrd atlhlatotvheerre avanlauleyszederaes
part of a routine, bi-assual vater testing progras
PEangveiro8syosfte1n7s Study Nusber 9063-3
RAM
002653
i
sree eres
Fst, is ines, ro tae
bude Envirosysteas Study Nusber 9069-3
TeH m O og
i |
AR
002654
STATISTICAL NETHODS: osctcaunrdraerddReastusittaalstlistotifecsatletdhetcoenctchoenxniitqcruiaettsyiont(seS.sttephacno,uld198m)otbecbaeuseint1e00r8prestuerdvivably
3
Eavirosystens Stuty Nusber 9065-3
Puce rotbe ico ARISE
8
Page 100 17
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002655
VIII. RESULTS
vcelsesaerlsaTnedsctonrteavmieansiisnneeglds6f1rc0eoenntgao/fi1niinangnsdol0u1bm,lg0e/010mnattgoe/rL4i0a0FlC-m7gt8/h7LrouFcgCoh-no7tu8at7inewtdheerefotaeismnti.otniTaeltslhtey
AdtpaeptsetandigmxeednieAar,atersdeusrpfeabccyteitvheetlhyar.couugtOhenoeuttohxuitnchedirteytdesttpe.esrtceBanirtoelospgurirecesaielvnatleadndocicwnuarTtraeebrdlequisa7litathneyi-
acnodntroal weetxpowsuerieg.ht (Cbolnottrtoeld of approximately 0.21 g/L.
dfriys)h
hofad 0.a3n2 agv,erargeesulttoitnagl
linengathloaofdin3g.4racnt;e
cpornecseennttreTadhteioinn2,4,Ttah48eb,le9672,h3.ouarndLBC9Se60cahuovsuaerlueLtCoSix5si0cs>if1to,yr0d0i0fdigshn/oLtexopPoco-os7ue8rd7.atto aFnCy-78t7estaerde
EPnavgeiro1S1ysotfea1s7 Study Nusber 9069-3
RosurcaAHncPiSs.inc.Snuesiyeof MUPORE
002656
Table 2. Survival data from toxicity test
--_--
ConNcoemnitnarlation (3g/L) rep.
-- Nusber Alive Obr 24hr 48hr 72hr 96hr
TNue sber MAfrfecTted Ohr 24hr dhe Thr 96nr
---- hr
Ofcontrol) 12 1100 1100 1100 1100 1100 00 0o oo oo oo
10
211010 1100 1100 1100 1100 00 0`0 oo oo oo
240
2101100 1100 1100 1100 11600 00 00 o0 oo oo
100
211010 1100 1100 1100 1100 00 00 oo oo oo
610
211010 1100 1100 1100 1100 00 o0 o0 oo oo
1,000
211010 1100 1100 1100 11000 00 00 oo oo oo
No_ te: ---- Suble-- thal effects excitability, discoloration,
ltheatthargwye,reandnotchaonbgseerivnedbehawveiroer. loss
of reflex,
PEangveiro1s2ysoftea1s7 Study Nusber 9069-3
RescoAnes.b.Stkdioy fMLPORE
002657
Table 3. Median lethal concentrations (LC50s) from toxicity test
T --E_px--eproisoudre o 150 n conf9i5depnecrecenltie nit calculad tLico5n0 method
24 hours > 1,000 ng/L,
-
-
48 hours > 1,000 mg/L
-
-
72 hours > 1,000 mg/L
=
po
96 hours
> 1,000 ng/L
-
-
_--_--m
PEangveiro13Sysotfea1s7 Study Nusber 9069-3
RescazceAnat.nc.Suk ofMLLPORE
002658
BEST COPY AVAILABLE
mw. aermamces
DLOiEatCbaDo.ratinory19th6Pe1r'.actAsiDsceeecs.issmieonnt.(o8f1)ofth3Ce0heC(aoFiuicnnacalilsl)..CoAnucneerxnin2.g thOeRCDKuPturailnciApclceesptaonfceGooodf
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OBFiEoDt.ic S1y9s8t4e.ms.GuideNlaitnheosd 2fo0r,TaTsisthingAcoufteChTeomxiiccailtsy.TesSte.ctioMnop2:edEffAepcrtisl o4,n
.
19.
USt.eSphaEnP,. SC.uEl.uth,198M3N.. PCeormspounatlerCoPmrmougnriacmatfioorn.the Calculation of LESO Values
PEnavgiero3s4ysoften1s7 Study Number 9069-3
fraseod oe sg mroRt
| 002659
AppendixA. VATERQUALITYDATAFRONTORICITYCEST
PEangveico1s5ysotfen1s1 Study Number 9065-3
RoceAnos. So MLSS
002660
BEST COPY AVAILABLE Table A.1. cCoonndcuecnttirvaittiyo,n apis,sutreesdperdautruirneg, taonrdicdiitsysotelsvted oxygen
conYcoennttnraalti~on
oMCsotnwdiusctiinvitwtiyoc(nmuimbnei/cona%o)
(g/l) Rep. hr he br hr br
RO.ow .. hoe abe hwe o:he hr
(control) 13 11330000 11550000 11550000 11330000 11330000 88.33 88..10 88..55 88..11 88..12
uo
12 11330000 11550000 11550000 11330000 11330000 88..22 88..00 88..55 88..00 18.1
yd
20
2113103000 11550000 11550000 11330000 11330000 88..22 88..00 88..44 88..00 88..00
"00
2113103000 11550000 11550000 11330000 11330000 88..11 77..89 88..44 17..99 18..90
600
2113103000 11550000 11550000 11330000 11330000 88..11 77..99 88..44 71..99 11..39
1,000
2113103000 11550000 11550000 11330000 11330000 88..11 77..99 88..33 17..88 71..98
PBaogveiro1s6yaoften1s7 Study Nusber 5069-3
Ta rsa ays
|
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|
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BEST COPY AVAILABLE
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Soicasptens Stuy Nosbr 3065-3
Page 17 of 17
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