Document N2rOpQreYm87VKxXB5jdR94pR

- Olo^i ACUTE TO XICITY TO FISH (RAINBOW TROUT) TEST SUBSTANCE________________________________________________ Identity: A mixture containing perfluorooctanesulfonate, which may also be referred to as PFOS, FC-95, or as a component of FC-3052. (1Octanesulfonic acid) (CAS # 2795-39-3). Rem arks: The 3M production lot number was not noted. The test sample is FC-3052. Current information indicates it is a mixture of 5% PFOS, 39% diethylene glycol butyl ether, 33.58% water, 10% Sultone foamer, 6% sodium octyl sulfate, 6% sodium decyl sulfate, 0.36% sodium lauryl sulfate, and 0.06% benzotriazole. The following summary applies to a mixture with incompletely characterized concentrations o f impurities. Data may not accurately reflect the toxicity o f the ftuorochemical component o f the test sample. METHOD:________________________________________________________ Method: Environment Canada Method EPS 1/RM/9 July 1990 Type: Acute static GLP: No Year com pleted: 1999 Species: Oncorhynchus mykiss Supplier: Spring Creek Trout Hatchery, Lewistown, MT. Analytical m onitoring: Temperature, pH, conductivity, and dissolved oxygen. Exposure period: 96-hours Statistical m ethods: LC50 values calculated by the Trimmed Spearman- Karber method, while the NOEC was determined via Fisher's Exact Test. Test fish age: Juveniles Length and weight: Length = 38 - 49 mm Average weight = 0.648 g (wet) Loading: 0.242 g/L Pretreatment: None Test conditions: Dilution w ater: Dechlorinated City of Duluth, MN tap water, post carbon. W ater was aerated for 24-hours prior to use. Dilution w ater chem istry: Hardness: 44 mg/L as CaC(>3 A lk a lin ity : 44 - 46 mg/L as CaCC>3 pH: 7.82 Lighting: Cool-white fluorescent lights. A daily photoperiod of 16 hours light and 8 hours dark was maintained throughout the testing period. Stock and test solution preparation: The test material was added directly to the dilution water on a weight/volume basis. 002911 Concentrations dosing rate: Once. S tability o f the test chem ical solutions: Not noted. Exposure vessels: 40 L glass aquaria containing 26.7 L test solution. Num ber o f replicates: 2 Num ber o f fish per replicate: 10 Num ber o f concentrations: five plus a blank control W ater chem istry during the study: pH range (0-06 hours): 7.36 - 7.82 (control exposure) 7.15 - 7.59 (125 mg/L exposure)* Tem perature range (0-96 hours): 14.4 - 15.4 C Conductivity range (0-96 hours): 1 2 6 -1 3 6 pmhos/cm (control exposure) 1 3 6 -1 4 4 pmhos/cm (125 mg/L exposure)* Dissolved oxygen range (0-96 hours): 5.8 - 9.1 mg/L (control exposure) 4.4 - 9.0 mg/L (125 mg/L exposure)* * 125 mg/L (intermediate concentration) exposure values used because the highest three concentrations used resulted in total mortality by 48 hours. RESULTS______________________________________________________ __ Nom inal concentrations: Blank control, 6 2 .5 ,1 2 5 , 250, 500, and 1000 mg/L Elem ent values: 96-hour LC50 = 1 7 7 (Cl not calculable) mg/L 96-hour NOEC = 125 mg/L Element values based on nominal concentrations Remarks: Testing was conducted on a mixture as described in the Test Substance Remarks field. The values reported apply to that mixture and not the fluorochemical proportion alone. C O N C L U S IO N S ___________________________________________________ The test sample 96-hour LC50 for rainbow trout was determined to be 177 mg/L. The 96-hour No Observed Effect Concentration (NOEC) was determined to be 125 mg/L. Subm itter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY___________________________ ________________________ Reliability: Klimisch ranking = 2. This study meets the criteria for quality testing. However, the study lacks information on purity of the test substance 002912 and it lacks analytical confirmation of the amount of fluorochemical proportion in solution. R E F E R E N C E S _________________________________________________ This test was conducted by AScI Corporation, Environmental Testing Division, of Duluth, MN at the request of the 3M Company, Environmental Laboratory, St. Paul, MN, Lab Request number W 2 0 3 9 -2 ,1999. OTHER______________________________________________________ ' L ast changed: 6/26/00. 002913 AScI Corporation Environmental Testing Division AScI Study ID# 50L0-068 STUDY TITLE ACUTE TOXICITY OF FC-3052 TO RAINBOW TROUT (Oncorhynchus mykiss) DATA STANDARD Environment Canada Method EPS l/RM /9 July 1990 STUDY COMPLETED July 19, 1999 TESTING FACILITY AScI Corporation Environmental Testing Division 4444 Airpark Boulevard Duluth, MN 55811 Tel. No. (218) 722-4040 Fax No. (218) 722-2592 STUDY IDENTIFICATION NUMBERS AScI Study ID# 5010-068 3M Lab Request# W2039-2 Sponsor: 3M Sponsor Study ID# W2039-2 1 002914 AScI Corporation Environmental Testing Division ASc! Study ID# 5010-068 1.0 GENERAL INFORMATION Study Title Data Standard Sponsor Sponsor's Representative Sponsor's Lab Request# Testing Facility Principal Investigator Project Director Testing Facility Director Definitive Test Dates Data Validation and Report Review Acute Toxicity of FC-3052 to Rainbow Trout (Oncorhynchus mykiss). Environment Canada Method EPS l/RM/9 July 1990. 3M Environmental Technology and Safety Services, Building 2-3E-09, 935 Bush Avenue, St. Paul, MN 55133. Susan A. Beach, 3M Environmental Technology and Safety Services, Building 23E-09, 935 Bush Avenue, St. Paul, MN 55133. Tel No. (651) 778-7452. W2039-2. AScI Corporation Environmental Testing Division, 4444 Airpark Boulevard, Duluth, MN 55811; Tel. No. (218) 722-4040. Joseph Dierkes. Joe Amato. Donald Mount. July 15-19, 1999. Alan Mozol. Report Signature: Retention of Raw Data and Final Report Location of Raw Data and Final Report Ten years from the date study is completed. AScI Corporation Environmental Testing Division, 4444 Airpark Boulevard, Duluth, MN 55811; Tel. No. (218) 722-4040. Sponsor: 3M Sponsor Study ID# W2039-2 2 00291 AScI Corporation Environmental Testing Division AScI Study ID# 5010-068 2.0 OBJECTIVE(S) To determine the 96-h LC10, LC50, and 96-h NOEC (no observed effect concentration) of the test chemical, FC-3052, for rainbow trout (Oncorhynchus mykiss) under static test conditions. This study was conducted according to Environment Canada Reference Method EPS l/RM/9 July 1990, "Acute Lethality Test Using Rainbow Trout." Test Chemical Test Organisms Dilution Water (PC Water) 3.0 TEST METHODS Properties: FC-3052, dark amber/brown colored liquid, specific gravity ca. 1.1 (water = 1), vapor pressure ca. 16 mm Hg calculated at 20C, stable, and miscible with water. Test concentrations: 0 (dilution water control), 62.5, 125, 250, 500 and 1000 mg/L based on range-finding test results. Test solutions prepared by direct addition to each replicate test chamber. Source: Healthy, juvenile rainbow trout (Oncorhynchus mykiss) obtained from a commercial supplier. Holding/acclimation: Held and acclimated for 24 days in the test dilution water. Less than 1% mortality occurred during holding/acclimation period. At test termination, they were 3.8-4.9 cm in length and 0.414-0.928 g in weight with a mean weight of 0.648 + 0.169 g. Loading rate was 0.242 g/L. Use in test: Did not feed 24 h before test initiation. Used 10 organisms/replicate and 2 replicates/test concentration. Source: Dechlorinated City of Duluth, MN, tap water. The most recent chemical analysis is in Raw Data Package. Characteristics: At test time, the water had a hardness of 44 mg/L (as CaC03) and a pH of 7.82. Aerated for more than 24 h prior to using in the test. Sponsor: 3M Sponsor Study ID# W2039-2 3 002916 AScl Corporation Environmental Testing Division AScl Study ID# 5010-068 Exposure Chambers Incubation Observations Endpoint Calculations Type: 40-L glass aquaria. Use in test: 26.7 L of dilution water or test solution/chamber. During the test, kept covered, except when experimental observations were made. Solutions were not aerated during the exposures. Duration: 96 h. Daily photoperiod: 16 h light and 8 h dark, maintained using cool-white fluorescent lamps. Temperature: 15 1C. _ Biological: Daily- Test organisms with abnormal behavior and signs of stress, and dead test organisms. Water chemistry: (1) At test initiation- total hardness and alkalinity, (2) daily- temperature, DO, and pH, and specific conductivity. All determinations were made according to APHA methods (1998). 24-, 48-, 72-, and 96-h lethal concentrations (LC50s), and 96-h LCl0 and no observable effect concentration (NOEC). LC50s determined via Trimmed Spearman-Karber Method, LC10 determined via EPA ICp Method, and NOEC determined via Fisher's Exact Test. 4.0 RESULTS Test Chemical Toxicity (Parenthetic values are 95% confidence intervals). Pertinent data are in Table 1. 24-h LC5q: 330 mg/L (301 mg/L-362 mg/L). 48-h LC50: 177 mg/L (Not Reliable). 72-h LCS0: 111 mg/L (Not Reliable). 96-h LCS0: 111 mg/L (Not Reliable). 96-h LCI0: 138 mg/L, Confidence Interval not Reliable. 96-h NOEC: 125 mg/L. Sponsor: 3M Sponsor Study ID# W2039-2 4 0 0 2 9 1 '? AScl Corporation Environmental Testing Division AScl Study ID# 5010-068 Ranges of Water Chemistry Parameters Total hardness (as CaC03 mg/L): 44. Alkalinity (as CaCO$ mg/L): 44-46. Temperature (C): 14.4-15.4. DO (mg/L): 4.4-9.1. pH: 7.15-7.82. Specific conductivity (fxmhos/cm): 126-185. 5.0 TEST QA/QC QA/QC Criteria Data Mortality in control exposure must not be more 0 % mortality. than 10%. The lowest DO concentration at test termination must be at least 60% of the air saturation value at test temperature. The lowest DO concentration measured was 44% of the air saturation value at 15.0C. The lowest DO concentration observed was at the 96 hour test interval at the 125 mg/L NOEC. The lowest DO concentration observed at the time of complete mortality in the 250 mg/L test concentration was 64% of saturation. The data show dissolved oxygen deprivation did not cause toxicity observed. Test duration must be 96 h. The test duration was 96 h. Reference Substance Toxicity Test (this test is Data package for the sodium chloride test with optional). this fish batch is in Raw Data Package. Sponsor: 3M Sponsor Study ID# W2039-2 5 002918 AScI Corporation Environmental Testing Division AScI Study ID# SO10-068 6.0 REFERENCES American Public Health Association (APHA). 1998. Standard Methods for the Examination of Water and Wastewater. APHA, Washington, D.C. Environment Canada. 1990. Acute Lethality Test Using Rainbow Trout. Conservation and Protection. Ottawa, Ontario. Report EPS l/RM/9. Hamilton, M.A., R.C. Russo, and R.V. Thurston, 1977. Trimmed Spearman-Karber Method for Estimating Median Lethal Concentrations in Toxicity Bioassays. Environ. Sci. Technol. 11(7): 714-719; Correction 12 (4): 417 (1978). U.S. Environmental Protection Agency. 1993. A Linear Interpolation Method for Sublethal Toxicity: The Inhibition Concentration (ICp) Approach. West Inc., & University of Wyoming, Laramie, Wyoming. 1996. Toxstat Software Package, Version 3.5. Sponsor: 3M Sponsor Study ID# W2039-2 6 002919 AScI Corporation Environmental Testing Division AScI Study ID# 5010-068 TABLE 1. ACUTE TOXICITY O F FC-3052 TO RAINBOW TROUT (O. mykiss) Test Chemical Nominal Cone. (mg/L) 0 (control) 62.5 125 250 500 1000 Cumulative Number of Dead Organisms and Percent Mortality 24 h 48 h 72 h 96 h 0(0) 0(0) 0(0) 2(10) 20 (100) 20 (100) 0(0) 0(0) 0(0) 20 (100) 20 (100) 20 (100) 0(0) 0(0) 0(0) 20 (100) 20 (100) 20 (100) 0(0) 0(0) 0(0) 20 (100) 20 (100) 20 (100) " Percentages are in parenthesis. EFFECT AND NO-EFFECT CONCENTRATIONS WITH CORRESPONDING 95% CONFIDENCE INTERVALS LCs and NOEC 24-h LC50 48-h LCjq 72-h LC50 96-h LCjo 96-h LCjq 96-h NOEC Data (mg/L) 330 177 177 177 138 125 95 % Confidence interval 301-362 Not reliable Not reliable Not reliable Not reliable Not applicable Sponsor: 3M Sponsor Study ID# W2039-2 7 002920