Document N2njZ2M23vMdgeMDjRJ1QGk7R

March * 1970 Dr. Oti# Faneher Industrial Bio-Tast Laboratory, Zne. 1810 Frontage Hoad Northbrook, Illinois 60062 Daar Otiai I belleva Beott Tuokar talked to you 00 the phone about redoing the fish toxicity work. This latter requests 96-hour flats) ba determined using the "standard methods" regardless of htv high the exposure levels have to be pushed to eause com plete mortality, fbe exposure levels we are talking about the amounts of the Aroolor added to the tank to reach the mortality levels. Z think it has been agreed that these studies should be carried out on Blue Sills and Bullheads at three Aroolor exposure levels) Dower eonoentratlon - all fish survive Median concentration - less than all fish survive Upper concentration - none of the fish survive with Aroolor# 12*2 (Lot Bo. AX-255). 125* (Lot No. AK-38), and 1260 (Lot Mo. AX-3). the samples whloh should be retained and shipped to Monsanto for PGB analysis should be ss follows1 Z. Zero time water samples (^50 ml) taken from esoh Aroolor-flsh exposure tank. OBample(a)/Aroolor/ Leva1/Apeole - (1X313X2) 18 Total! and 1, 2, 3# 1 6, 12, 2*. *8, 72 and 96 hour watar samples k-50 ml) from sash of the lowest level Aroolor exposure tank# (9X3X122) - I^TotaQ. OSW 034656 STLCOPCB4018483 Dr. OtlB Fancher March 4, 1970 Page Two II. All fish mortalities. III. We would also like to have blank exposure tanks (no fish) set up exactly as the tanks containing fish, at the same time, and 0, 1, 2, 3, 6, 12, IV. Additionally, two blank tanks containing fish but no Aroclors should be set up and water samples (^50 ml) taken at 0, 1, 2, 3, 6, 12, 24, 48, 72 and 96 hours and the fish sacrificed for analysis Qsample(s)/species) * 10X2 20 Total]]. The water samples should be taken in previously cleaned amber glass vials and sealed with foil lined screw caps. Fish mortalities and sacri fices should be washed with distilled water, blotted dry* and quick frozen in poly bags. We would also suggest that the exposure tanks be washed thoroughly and not be re-used. If there is anything that we can add to the above or if you or Carmen have additional comments, please call Scott Tucker. Best personal regards. * Sincerely, Elmer P. Wheeler Manager, Environmental Health P W * 1 li c.c*. Dr. Joseph C. Calandra b.c. M. W. Farrar R. E. Keller/E. S. Tucker W. B. Papageorge Wo R. Richard DSW 034657 STLCOPCB4018484